LOCUS       DAOSOC010000001        58438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_577_length_58438_cov_9.446928, whole genome shotgun sequence.
ACCESSION   DAOSOC010000001 DAOSOC010000000
VERSION     DAOSOC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 58438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58438
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_577_length_58438_cov_9.446928"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3158
                     /gene="carB"
                     /locus_tag="PL014_00005"
     CDS             <1..3158
                     /gene="carB"
                     /locus_tag="PL014_00005"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOT55500.1"
                     /translation="TIMTDPEFADATYVEPITPEIVEKIIAKERPDAVLATLGGQTAL
                     NCAIELHERGVLEKYDAPLIGANVEAIELGENREKFKGVVERCGAESARSVICHTMGE
                     VLAAADDLGYPVVVRPSFTMGGLGSGFAHDEDELVRIAGAGLQYSPVTEVLLEESILG
                     WKEYELEVMRDHADNVVVVCSIENFDPMGVHTGDSITVAPALTLTDREYQRLRDVGIA
                     IIREVGVDTGGCNIQFAINPADGRLIVIEMNPRVSRSSALASKATGFPIAKIAAKMAI
                     GYTLDEVRNDITRETPASFEPTLDYVVVKVPRFAFEKFPAADDTLTTTMKSVGEAMAI
                     GRNFTEALQKALRSLEKKGSSFHWDGERPTTEQAREILAVATRPTDGRIIFVQQALRG
                     GLSVEEVHEATGIDPWFLDQMALIEQIADAVAAAPELTPELLRLAKTHGFSDAQIAGL
                     RAMPEAVVRGVRHALGVRPVFKTVDTCAAEFAAQTPYHYSTYDEENEVAPRERPAVLI
                     LGSGPNRIGQGVEFDYSCVHASFALRDAGYDTVMVNCNPETVSTDYDTSSRLYFEPLT
                     LEDVLEVVHAERHAGPIAGVIVQLGGQTPLGLAKALKAEGVPIVGTSPEAIDLAEDRG
                     AFGRVLHEANLPAPKHGTAYSAHEAVDIAREIGYPVLVRPSYVLGGRGMEIVFDDETL
                     SGYVERATIASPEHPVLVDRFLDDAIEIDVDVLYDGVDIYVGGIMEHIEEAGIHSGDS
                     SCTLPPVTLGDSELERVRVSTRKLAEGIGVRGLMNVQFALAQDILYVLEANPRASRTV
                     PFVAKATGVPIAKAAARIMLGATIAELRDEGLLPAHTDGGRMPAHAPMSVKEAVLPFK
                     RFRTADGLVVDSLLGPEMRSTGEVMGIDTDFGRAFAKAQLGAISGLPTTGTVFVSVAN
                     RDKRAIIFPVKRLLDLGFRIVSTSGTADVLRRNGIEAEVVLKHAERGPEGRSIVDWIN
                     AGEVDMVVNSPSGPSASARADGYAIRAATTAMDKPIITTVQQLAAAVQAIEAVITGDL
                     DVTPLQQHAADLDLYGVQAPA"
     gene            3155..4045
                     /locus_tag="PL014_00010"
     CDS             3155..4045
                     /locus_tag="PL014_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOT55501.1"
                     /translation="MSAASVAPAAGGVTQVVGSVVANDPVGAYRHLVISAPGVAELAS
                     PGQFVALAVGGPTSANLLRRCFSLHRVDVGLGTIEVVIAAHGPGTTWLTEVRVGDPVD
                     VVAPLGRPFPLPEQPEACVLVGGGYGSAPLFWLAEALRTRAGHVEMVLGAASEDRIFG
                     ASLARRDADGVTVTTDDGSIGRRGWVSVVLGDVIDRTNATVVYACGPMAMLKSVTDIA
                     TAHGAVAHVAVEESMACGIGVCMTCVLPITGEDGVTKMVRSCVDGPVFRGDRVRWDAF
                     GDGICRVPADTLGAPQAGGH"
     gene            4054..5004
                     /locus_tag="PL014_00015"
     CDS             4054..5004
                     /locus_tag="PL014_00015"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOT55502.1"
                     /translation="MSTALGALTLPNPVMTASGCAANGQELNRFFPVSELGAFVTKTV
                     MAAPRSGRGTPRMAETASGMLNSIGLQGPGIEAFVENDLAWLASVGARVLVSIAGNTA
                     AEFGEVAACLADSPAFAAVVGVEVNISCPNVANRGLVFACDPVSSDAVVRLVRDKLPA
                     ATPIFAKLTPDVTDIVTIADAALGAGADGLTMINTLLGMVIDTDLLRPQLAGVTGGLS
                     GPAVRPVAVRALWQVTAAMRDGRLPTAPLIGVGGVRTGRDALELVAAGASAVQVGTAT
                     FNDPSRPSRVVDELEAEVARHGFTRFADVVGIAHDRMVSP"
     gene            5001..5852
                     /gene="pyrF"
                     /locus_tag="PL014_00020"
     CDS             5001..5852
                     /gene="pyrF"
                     /locus_tag="PL014_00020"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT55503.1"
                     /translation="MSLDPFGVRLRAAMDAHGPLCVGIDPHAALLQAWGLPVDLSGLE
                     RFAMTCVEAFAGRVAVVKPQSAFFEVFGSRGVAVLERVLADLRDAGTLTILDAKRGDI
                     GSTMAAYAQAFLSDEGPGRADALTVSPFLGYGSLRPALDVAAATGRGVFVLALTSNPE
                     GPQVQHAQTPQGSVAGAVVAGAAADNAGAAARGEMGHVGLVVGATVGDAVTRLGLDLA
                     SSAAPLLAPGLGAQGAGPGDLRAVFGDAVGNVLPNVSRSVLSAGPSVQALAAAAARDA
                     DSVRAAL"
     gene            5928..6242
                     /gene="mihF"
                     /locus_tag="PL014_00025"
     CDS             5928..6242
                     /gene="mihF"
                     /locus_tag="PL014_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HOT55504.1"
                     /translation="MALPPLTPEQRSAALAKAAASRRERAEVKHRLKHSGLSLAEVID
                     AGKTNEVVGKMRVSALLESLPGVGRVRARQIMQEVGISESRRVRGLGQNQAAALIERF
                     GR"
     gene            6239..6805
                     /gene="gmk"
                     /locus_tag="PL014_00030"
     CDS             6239..6805
                     /gene="gmk"
                     /locus_tag="PL014_00030"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOT55505.1"
                     /translation="MSAPSRLTVLAGPTAVGKGTVAAYVREHHPEVWVSVSATTRRPR
                     PGEIDGIHYHFVDDEHFDAMVRDGELLEWAVVHGRAKYGTPRGPVEQALAAGRPALLE
                     IDLQGARQVRETMPEALFVFLAPPTWDELVRRLVGRGTEGEEEREARLSTARVELASA
                     SEFDVTVLNDDVHRAAEELVSLMRTPPT"
     gene            6823..7092
                     /gene="rpoZ"
                     /locus_tag="PL014_00035"
     CDS             6823..7092
                     /gene="rpoZ"
                     /locus_tag="PL014_00035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOT55506.1"
                     /translation="MSGTVADPIGITNPPIDELLERADSKYSLVLYSAKRARQINAYY
                     AQLQEGLLEYVGPLVDSEVHEKPMSIALREINEGLLTSTPTPSQG"
     gene            7119..8402
                     /locus_tag="PL014_00040"
     CDS             7119..8402
                     /locus_tag="PL014_00040"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0071513 - phosphopantothenoylcysteine
                     decarboxylase complex [Evidence IEA];
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate synthase"
                     /protein_id="HOT55507.1"
                     /translation="MKVVLGVAGGIAAYKAASLLRLFTEAGHDVTVVPTQAALNFVGA
                     ATWSALSGKPVHTDVWSDITAVPHVRIGQEADLVVVAPATADLLARAATGQANDLLTT
                     VLLTARCPVVMAPAMHTEMWEHAATQANVATLSERGVHIVPPASGRLTGSDTGPGRLP
                     EPEDLFAVCARIVPTTPAGDRAPHRADAVPEAAGRKGEDLTGLRVVVSAGGTREPLDP
                     VRYLGNRSSGKQGYALAQAAADRGADVTLVSSSSQPVPAGVTLVPVETALELQAAVTH
                     AVHGADVVVMAAAVADFRPSSYADHKIKKSHEAAADGSADESAPVVTLVRNPDILAGL
                     VEARGSATTPVIVGFAAETGDGEASVLEHGRAKLARKGCDLLVVNEVGVGVTFGQDDN
                     TVHILERGTDHVTDIGPATKLEVAHAVWDAVTDLL"
     gene            complement(8471..9673)
                     /locus_tag="PL014_00045"
     CDS             complement(8471..9673)
                     /locus_tag="PL014_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55508.1"
                     /translation="MNQPEFPTVFRGYDPVSVDQHVSALRQAAEAARQAAEAAQQEAA
                     SASVSLTKLRQSHDALMQEVDGQRRSVAELEEHARRASNPSFADLGERIGSILTLADE
                     EAAAIRATAEEEAGQTRFAADEAAALARTEADRYAEDRRSRADVDAEQALARAKQEAD
                     SIVDDAAREAAARREEAEAYFEKQRATAAASAADFERTLAERREKATAEFTGQMAKQD
                     QALAAVQERADLLAREAEQDRAAAAEEAARVLDAARSEAAALVASAKEQAERVRRDSE
                     RELSAATARRDSITAQLSNVRNMLATLGGPAAVTALDAAADADAPAEQAPAEDAEQAP
                     AAESVDTSEATAEADGDHPASETGEVDGEQAASETAEDDATADDQGSDESDRVPGSAR
                     KAKTSARR"
     gene            complement(9673..11118)
                     /locus_tag="PL014_00050"
     CDS             complement(9673..11118)
                     /locus_tag="PL014_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55509.1"
                     /translation="MSDRSVNPEAAGDAGGAASADPAAEALSLLTRAQALADDLRLEA
                     HREVVSARDEATRLRAEAQEAGHELDDLRAGIAAARAEAARRLEETRAEVEIMLADAA
                     DQAALLRARATKASDEALGLATEQAQAELAQARTAAEAILEQARREHELTVARLADDE
                     RAHQQRWSSEHTAHTTELHALRESTLAELDGLRREAEQFSLTHRGEAQARAAEVLAAI
                     GERDEASRAAAESLVSGAKAHADELLADARAEADSVMAAAQAHLAWAQATVRELVTGA
                     RADAEQRARTHHRELADRSRAHRQQVQTLLAAATAKGADRLSEADSEARRLREQAEAV
                     LAEAQADAARTREAAARESQRVVDEAQQRAEEALERATRRTTEAEQGAALVRERAAAD
                     VERMHREAHEHHRAARAEILRTSEDARARAEAVRAEAQAAVERARAEVHTLAARRDAI
                     TAQLGSLSGVIDALAVPEDPPHPQTPPHQES"
     gene            11244..12464
                     /gene="metK"
                     /locus_tag="PL014_00055"
     CDS             11244..12464
                     /gene="metK"
                     /locus_tag="PL014_00055"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT55510.1"
                     /translation="MSARLFTSESVTEGHPDKICDQISDSILDAMLAQDPGSRVAVET
                     MVTTGLVHVAGEVTTEAYVEIPKIVRDTVLAIGYDSSTKGFDGASCGVSVSIGQQSPD
                     IAQGVDTAFESRTGAVDPLDKQGAGDQGLMFGYACDDTPELMPLPIFLAHRLAERLTA
                     VRKNGELSYLRPDGKTQVTIAYDGDRAVSLDAVVVSSQHAEDISLEGMLEPDVRAHVI
                     DPVLAELADSGTELHTDDYRAYINPTGKFVIGGPMGDAGLTGRKIIVDTYGGMARHGG
                     GAFSGKDPSKVDRSAAYATRWVAKNVVAAGLARRCEVQVAYAIGKAQPVGLYVETFGT
                     ETVAADKIQAAILATFDLRPAAILDALDLLRPIYKSTAAYGHFGRTTAAGVDNPFTWE
                     RTDRVEALQRAANA"
     gene            12539..14572
                     /locus_tag="PL014_00060"
     CDS             12539..14572
                     /locus_tag="PL014_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775101.1"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HOT55511.1"
                     /translation="MAVTGDPTSEVLPVALVQVDTGLAHLDRPFEYTVPTSSADTALP
                     GVRVKVRFAGQDLSGFVLDRRADAEHLGRLSPIRRVVSPEPVLTPAVLRVARVVADRY
                     AGSLGDVLRLAVPPRHAAAEKALPASSPDPADRPPLPDRMPDAWLPYPAGSAFLTHVG
                     AGGAPTASLLAVPSTDPDADWPMLLAQAARTAVEGGRGAVIVVPDHRDVVRVEAALTR
                     SLGPGRHTRLTADQGPQARYTAYLKALRGHAQVVVGTRAAAFAPVRDLGLVAWWDDGD
                     DLLDEPRAPYPHVREVLLARAADEGAAVLAAAYSRSVAVADLVSRGVLTPVEAPRAVV
                     RAAVASVRVAGEGHDADRDPAVAAAHLPSLAWRTAKEALAHGPVLVQVPRRGYLPSLS
                     CQTCRRPARCSACAGPLALAGPQGPTSCRWCGRVETAFTCPACGGHALRSAVVGARRT
                     AEELGRAFPGVPVERSGAGTVLDVVTSGPRLVVSTPGAEPVAPEGYAAALLLDAWALL
                     DRPTLLAGEEALRRWLAAAALVRPASGGGRIVLAGAPTDVSVPAVEALVRWDPTWFAE
                     RELTERRELSLPPAARLATLTGPRAALERAVAEVGLPASAAVLGPLPHAGAVGGAGPA
                     GPAPQERWRTIITVPRPDGPDLTRELAAMRARASARKDPDTITVRVDPPDPTT"
     gene            14624..15166
                     /gene="def"
                     /locus_tag="PL014_00065"
     CDS             14624..15166
                     /gene="def"
                     /locus_tag="PL014_00065"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT55512.1"
                     /translation="MSVTPIRLFGDPVLRTPAEPVVDFDAELRRLVGDLTDTMLDAGG
                     AGLAAPQLGVGLRVFTYHVDGVIGHLVNPDLSLGEDEERDLEGCLSLPGLHFETPRAV
                     QVVARGWTMHGEPATVEGTHLLARAVQHETDHLDGILFIDRLDPEQRREAMRLIREAD
                     WGATPPVVRASPHAGAFGRR"
     gene            15168..16136
                     /gene="fmt"
                     /locus_tag="PL014_00070"
     CDS             15168..16136
                     /gene="fmt"
                     /locus_tag="PL014_00070"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT55513.1"
                     /translation="MRLVFAGTPVTAVPSLLALLASPHDVVAVLTRPDARSGRGRTLA
                     PSPVKEVALEHGLEVLTPVSPRDPDFHARLVELDPDCCPVVAYGALVPRAALDVPRVG
                     WVNLHFSLLPAWRGAAPVQRAIMAGDDVTGATTFLLEEGLDTGPVLGTLTETVRPSDT
                     SGILLDRLAHAGAGLLVATMDGIADGSLHAVPQPDDGVSHAPKLTTDEAQVRWGLPAH
                     VVDRHVRGCTPAPGAWTTFRGERVKIGSVGLTAAAARMPSADFVDAPSLALAPGELHV
                     TKRSVLVGTGSTPVELREVRPHGKKVMAAPDWARGVRITPGERFDG"
     gene            16129..17670
                     /locus_tag="PL014_00075"
     CDS             16129..17670
                     /locus_tag="PL014_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT55514.1"
                     /translation="MAEREHHDRSGSRPQRRDDRAGGRPASGGGRGPRARSATAPSQR
                     TRRAEPTRFAAYTLLRSVAEGGYANLELPDILRRHHLAGRDAAFATELAFGTIRWQGF
                     YDAVIAEAAGRPTERIDPQVLDVLRLGAHQLMGMRVSTHAAADQTVGLSKVVAGAGAG
                     SFVNAVMRRMSERTLEQWQEAVVPEGGGVDALAVRHSHPQWVVSALRAALLGHGRATA
                     ETIDTELEALLAADNDPPRVHLVARPGLSDVDELLSSAEARKSTLSPHGVVLESGDPG
                     AVAAVRQGRASVQDEGSQLVALALAAAEVGPDVPDRWLDLCAGPGGKAGVLGAMAVQH
                     GADLTAVEVTPHRADLVRTTLAALTRHAESVGQRVEVRTADGRDVGGEEPGGYGKVLV
                     DAPCTGLGALRRRPEARWRRQPSDVAELAPLQRALLTSALAAVAPGGVVAYATCSPHV
                     AETRFVVSDVLKKHPDVEVVDARPLFTDASGAPVDGLGDGPYVQLWPHVHGTDAMFFA
                     LLRRR"
     gene            17698..18357
                     /gene="rpe"
                     /locus_tag="PL014_00080"
     CDS             17698..18357
                     /gene="rpe"
                     /locus_tag="PL014_00080"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT55515.1"
                     /translation="MLISPSILSADFANLERELGAIANADWAHVDVMDNHFVPNLTLG
                     LPIVEALVRVSPIPIDCHLMIEDPDRWAPGYAEAGARSVTFHIEAVADAVATARAIRA
                     AGARAAFAVKPGTPFAPYEDLLPEVDMVLVMTVEPGFGGQSFMADQMSKVAQVREAVR
                     RHGGEVWVQVDGGVSASTIEQCADAGADVFVAGSAVYGAESASAAIEQLRALATAHTH
                     R"
     gene            18557..20560
                     /locus_tag="PL014_00085"
     CDS             18557..20560
                     /locus_tag="PL014_00085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT55516.1"
                     /translation="MDVHAALTDVRSSATILDGMRAGERLEDVARSAGPEVVDHLVVA
                     TTDPDPITAIAAVHALGAHGSATAGAHLVSLLDDERAHVAEHAIDALSGVVAQAAALE
                     RLVRHCAEGGFPGMLAARTLERWAAASPDAVEHAVTVGLASERDPAARAHLEELAQDL
                     RSGARDATKPPTRGGPGLTVAQLFLHGDLDGSLRHSGQGDTGGVATLLVHLGDALLRH
                     GARVDRVITISGSRDAAVADAWAAGATTGRTLSQDLAPRGHHYARIPLSGPSTSVSTA
                     WSLRIQARHGIREVLRAAGRVDVIHLRMADVGSLVAAEVAAELGIPVVLTLAPDPQAL
                     LAAREADGSLTRAEFGDADRVEHLWFRDRLLRALAAQAEHLVLFPRPDVERSIGRLVG
                     IDVTAQRERVSVVGEGIDLAALERVAKEVAGDAPRSPETASALRELSAVLDQLPPNRR
                     GLPLAVSVGRLSPVKGMATLASTWLTEPTLRERCNLVIVGGDLDDPTTEEAAELARIR
                     EVVSTDDCAGEGLLLAGHRPNATATAWLAAAHRGVPGVCAPDGVYVCASLKEEFGIAI
                     LEAMTMGLVVVAPNSGGPATYIEDGVTGVLTDTTDPRRLADAFGRALELASSPGEALR
                     TRRSRAGVRERFGIDTMASSLVEVYAAVAARQAATSLPRAVTS"
     gene            20557..21864
                     /locus_tag="PL014_00090"
     CDS             20557..21864
                     /locus_tag="PL014_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT55517.1"
                     /translation="MTLLVISPDYASHLYPLATLATAWQAEGERVVVATGAATDAVVR
                     AFGYDREHLQLGRGSNPGVIRAEEQPPGEDDALRGFFDATRRGAVAALEFQARARGDD
                     LLWNAVGVAREVQAIVERVQPDAVIVDHLAFGARLGLVGAQVPHADVVLGHPTALTVG
                     DEVYGHPPTWPRAMRPADEELADLRVLCEGVRDTFTAQWNAALKDLRPSAQPSRDAFA
                     ETGDLLLLNYPAALHEPERTALLPPHTFLGSAVREEPRDAQVEAWLDADEGSVVYVSL
                     GSFLSARSDVLALIADALRRLDVRVALATGSAPVEELGPLPQGWLVREFLPQVRLLAA
                     SSVAVTHGGNNSVTEALTAGVPLLVLPFSTDQFAGAAAIERTGVGVCLDPNATTSGDI
                     ADAVRHLLAPGGARSAAAAIGEGLREVPGPQRARAAVRQALSG"
     gene            complement(21854..22675)
                     /gene="xdhC"
                     /locus_tag="PL014_00095"
     CDS             complement(21854..22675)
                     /gene="xdhC"
                     /locus_tag="PL014_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HOT55518.1"
                     /translation="MHWLAALDHLRTSRQPGVLVTVTRVRGHAPRDAGAKLVVSDRDT
                     WGSIGGGNLEATAVERARRMLADGADVPEQLDITLSERARTEHGRQCCGGEVSVLLEP
                     LAVVPCVAVFGVGHVGHELARILSRHDLDLHLVDSREEQLDVAMMSWLGEAVARVERH
                     HSPLPEMVLDSLPAGTHVVVMTHDHAEDYALCDAALRRAGLGSVGLIGSSAKWTRFQQ
                     RFAADGHPAHVVNRIHSPIGVPDLAGKEPASIAVAVAADLLQVISRGHRSADVSR"
     gene            complement(22677..24980)
                     /gene="xdhB"
                     /locus_tag="PL014_00100"
     CDS             complement(22677..24980)
                     /gene="xdhB"
                     /locus_tag="PL014_00100"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HOT55519.1"
                     /translation="MSAVAQGPDDQVVGQPLPHESAALHVTGRALYTEDLGSRMPGLL
                     HAHPVQAPHAHALVTRLDAEPALSVPGVVRVLTAADVPGINDAGAKGDEPLFPDEVMF
                     HGQSVCWVLAETAEAARLGALAVEVEYEVLPAVVTVEEAIDAGSFQGGAPRLERGDVE
                     SGLAQAAHVFSGVTTSAGQEHFYLETHCSIAHVDESGQVFVQSSTQHPSETQDIVAHV
                     LGRATHAVTVQCLRMGGGFGGKEMQPHGFAAVAALGAVITGRPVRVRLTRGQDMTMTG
                     KRHGFHTRWQVAFDDDGHLLALEATLTSDGGWSLDLSEPVMARALCHVDNAYWIPNVR
                     VDGRIARTHKTSQTAFRGFGGPQGMLVIEDILGRCAPLLGIDPLSLRRRNVYREGQST
                     PYGQVVRHAERIVTCWEQVLSSGDVARRLEQVAQYNASHEHSKRAIAVTPVKFGISFN
                     FTAFNQAGALVHVYKDGSVLVNHGGTEMGQGLHTKMLQIAATALDVPLDLVRLAPTRT
                     DKVPNTSATAASSGADLNGGAVLDACVQIRERLRAVEAAHAEPLPWADLVAVAYADRV
                     QLWAAGFYRTEGLHWDAAAMQGSPFKYFSYGVAAAEVEVDGFTGSHRVLRVDVVHDVG
                     DSLSPLIDLGQVEGAFVQGAGWLTLEDLRWDESDGPSRGRLATNTASTYKLPSFSEMP
                     EEFNVTLLERAHEDGAVYGSKAVGEPPLMLAFSVREALRQAAAAFGPPGVSVGLATPA
                     TPEAVFWAVEHARGGGVPPGLHPVVEG"
     gene            complement(24977..26428)
                     /locus_tag="PL014_00105"
     CDS             complement(24977..26428)
                     /locus_tag="PL014_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOT55520.1"
                     /translation="MATNQTPRVTVNGVIHDLAGTDPWTPALDWLRSIGLTGAKEGCA
                     EGECGACSVMLARRDGDDRTRWAAVNACLLPASALAGQEVVTSEGLGSPEALHPVQHE
                     MAVRGGSQCGYCTPGFVCSMAAEFYRPDRAPSPKDHGGEVGPNGFDLTAISGNLCRCT
                     GYRPIRDAAFALGLPQTDDALASRLEGPGPAAAPVVVDGPTGSYVRPADLDEAIHLLQ
                     RHPDAVVVAGSTDWGVEVNLRGARAELVVGVDHLAELRAVTRGADHLEIGAAVTLAEL
                     ERGIAREVPLLTAVLPLFASPLIRNSATIGGNLATGSPIGDLAPALLALEADVVLHGT
                     DGQRLVPLASFFTGYRRSVRRRDELIRAVRIPLPLGEVTGFQKIAKRRHDDITSVAVA
                     VSLHVVDGSVQRARIGVGGVAATPLRATDTEAALEGRAWTRATAAAAGEVLRDVGTPI
                     DDHRASAAYRRAVLATALVRLHAQGASTPEVTR"
     regulatory      26549..26679
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            26801..27838
                     /gene="ribD"
                     /locus_tag="PL014_00110"
     CDS             26801..27838
                     /gene="ribD"
                     /locus_tag="PL014_00110"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOT55521.1"
                     /translation="MDESTALMQRAIDAARLGPQADPNPRVGCVVTDAAGRVVGVGHH
                     RGAGTAHAEVDALAQAGEAARGGTAHVTLEPCNHTGRTGPCTGALLEAGIARVVFAVG
                     DPDAVAAGGAATLAAAGVAVETGVLREAAEELNESWLHARRTGRPFVIAKSASTLDGR
                     TAAADGTSRWITGEAARADVHDLRSRCGAVVVGTGTALSDDPQLTVRTADGTAAGSQP
                     LRVVVGHRTLPATARVLDGAAETLLLATHDPGAVLAALHARGIHRVLLEGGPTLTAAF
                     LAAGLVDEVVAYVAPALLGSGPTVSADLGVRTIADAVRLDLVDVTVLPPDVRITARPA
                     TAPPVHPRARS"
     gene            27844..28497
                     /locus_tag="PL014_00115"
     CDS             27844..28497
                     /locus_tag="PL014_00115"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOT55522.1"
                     /translation="MFTGIVEELGTVLALDTLEADGRTDSARLTVAGPLVTADATHGA
                     SISVNGVCLTVLTHGDGEFTVDVMAQTLALTSLGALRPGDRVNLERAMAADGRFGGHV
                     VQGHVDGVGTIVRRTPGERWEVVEVSLPADLAGYVVAQGSITVDGVSLTVAGLTGTTF
                     SVSLIPTTLALTTLGTKDVGDPVNLEVDVLAKYVERLLATRFASPAAQPTPTPAEES"
     gene            28494..29717
                     /locus_tag="PL014_00120"
     CDS             28494..29717
                     /locus_tag="PL014_00120"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOT55523.1"
                     /translation="MTLSTIEAALDALRLGRPVLVLDDEDRENEGDVVLAGQTLTDQW
                     MAWTIRNTSGYICAPVTEEVADRLDLPLMVRDNRDPRRTAYTVTVDAASGVSTGISAA
                     DRAHTIRTLSDPTASADDLIRPGHVVPLRARAGGVLARAGHTEAAVDLCRLAGLEPVG
                     AIGELVADDGTMLRTPGVLSLGAAHDLPVVTIADLVAWRQRHDRVTRVADTVLPTRHG
                     TFRTVAYRDVLTGVEHLALVSPLGIPERAPLVRVHSECLTGDVMGSQRCDCGPQLERS
                     LERVAAEGGVVVYVRGHEGRGVGLGAKLRAYELQDRGLDTVDAQIELGLPIDAREYAA
                     GAAVLLDLGVHELRLLTNNPAKVDAMREHGIRVVAVERLRIAPVAENAAYLRTKRDRM
                     GHDLVIDSSEGAAPA"
     gene            29714..30199
                     /gene="ribH"
                     /locus_tag="PL014_00125"
     CDS             29714..30199
                     /gene="ribH"
                     /locus_tag="PL014_00125"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOT55524.1"
                     /translation="MSGHGAPVVTVDGRGLRVAVVAASWHEQVMDGLVDGALRGLADA
                     GVQSPLVVRVPGTVELSVACARLAPRFDALVALGVVIRGGTPHFEYVCQGVTVGLTQV
                     SVTTGVPVGFGVLTCDTDEQAVARAGLPGSVEDKGHEAATAAVATAVTLRDLDAAAAG
                     R"
     gene            30205..30930
                     /gene="pnuC"
                     /locus_tag="PL014_00130"
     CDS             30205..30930
                     /gene="pnuC"
                     /locus_tag="PL014_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOT55525.1"
                     /translation="MRELLAWLQHESITVAGYPLPWIEVVGNLFGFASAILGMRRKVW
                     AWPIGIVGNLLLFTIFVSATFDADSRTPLFGQAGRQVFFIMTSLYGWWRWQEHRNARL
                     AAGASADTPAITPRWATARERLGYVLVWIGGVLVAQWALAGIGAAWPAPRWYFWCDAW
                     ILVGSMLATYAMARGWNEFWLMWIAVDLVGVPLLWHSEYYATAVLYGVYGLLVLYGFF
                     VWLRASRAEAPDALETRELDPVA"
     gene            30983..31246
                     /locus_tag="PL014_00135"
     CDS             30983..31246
                     /locus_tag="PL014_00135"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOT55526.1"
                     /translation="MKTFDELFAELADKARTRPPGSGTVTELDSGVHGIGKKIVEEAA
                     EVWMAAEYEGRERTAEEISQLLYHLQVLMLACDVTPDDVYAHL"
     gene            31314..32159
                     /gene="hisG"
                     /locus_tag="PL014_00140"
     CDS             31314..32159
                     /gene="hisG"
                     /locus_tag="PL014_00140"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOT55527.1"
                     /translation="MLRIAVPNKGSLSEPSAAMLREAGYRTRRDPKELIVTDAANGVE
                     LFYLRPRDIAVYVGSGTVDCGITGRDLLLDSGSAAVEVLALGFGASTFRYAARPGTAS
                     ALADIGGHRVATSYQGLVEKHLADHGIQAEVVRLDGAVETAITLGVADVIADVVETGA
                     TLRSQGLEVFGDPILKSEAVLVRREGSEEAAQVEVLRRRLLGVITARHYVMLDYDVPA
                     ALVDAACAITPGLESPTVSNLQNRDWCAVRAMVPRSGTNAVMDELYDLGARAILVTDI
                     TACRL"
     gene            32156..32602
                     /locus_tag="PL014_00145"
     CDS             32156..32602
                     /locus_tag="PL014_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT55528.1"
                     /translation="MTDTGGSSGPVVFRPRRGRVVPLVMATIALVVTAGVALGMGGAG
                     WSGGDQLALFGLGAGLAAFLGRYASIRAVGDDVGLTVRNLLITRTVSWEEIVEVRFPD
                     GAPWVSLELTDTDELAVMAIQRADGDDAKVEARRLAELVAERRTTR"
     gene            complement(32609..33778)
                     /locus_tag="PL014_00150"
     CDS             complement(32609..33778)
                     /locus_tag="PL014_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT55529.1"
                     /translation="MAAIVLVAANMRTLMASLPPLAEAVRADLGLSSAWMGVLTTLPV
                     LCMGLLAPGANQVARRLGSEVATGAGVGLVLAGLVLRGSGGGAVWSLYAGTFVAGAGI
                     ALAGTLLPGIVKAVFPARRSGLGTGLTMAAMMGLAAVASATSVPLADVLGDWSRSLLV
                     WTPLAALALVVWVPVARAAHRHPEREEVPEDVTHALPWASATAWLVAAYLTAQSWQFY
                     SALAWLAPTYEAHGWTAREAGLLMAAFTGAQLLSGLAAPTLLDHVPDPRLLLVLAGLL
                     GGAGELGVWLAPDTAPWLWAGLLGAGQGAAFALGLALLVRYAATPRDSARLTAMAFLV
                     SYTAAAFGPAAMGVVEDVTGSFSLVWALLALVMVPQLILSLRLRPDLARVGAQID"
     gene            complement(33802..34881)
                     /locus_tag="PL014_00155"
     CDS             complement(33802..34881)
                     /locus_tag="PL014_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HOT55530.1"
                     /translation="MRFPDARPALARGLDRLADRWVLLLQIGVSVFVAWLVASEVLGH
                     VNPFFAPVTAILCLGLTYGQRLRRIVEVTVGVAIGILVGDLIVVWLGSGAVQMALVVV
                     LAMGIAVVVSSGQLLVIQAGVQSVFIVAFVADQSASFSRWLDAVVGGAVALLVGAIAP
                     TSPVRRPRVLAARTAREIATTLREVVDALHRRDRALATAALDGARALEDQLAELRVAA
                     SEGAAVVRQSPLLRGHAPAARAGSALVVPLDRCVRNLRVLARRAVVALVRGEAVPEQY
                     IALVAALADAAGDLARVLDEHATPSAARAVLDRVAELSAHVDPDAGLSAEMLRGQVRS
                     MVVDLLVIAGMDEDDALLSLPESSA"
     gene            complement(34878..35462)
                     /locus_tag="PL014_00160"
     CDS             complement(34878..35462)
                     /locus_tag="PL014_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55531.1"
                     /translation="MSQPPAEHADVADPAHVAAVLAVAADAAPVAGPTVVVAIDGRSG
                     SGKTMLGTAVADALGCPIVHLDDIFPGWDGLSAGVHLVTEQVLEPLARGGSAAYQRWD
                     WMRSRPGRTIDVAASAHLVLEGCGALVPPALQHAAVRVWVEAPDAVRRRRALSRDGDV
                     YAPHWQRWAVQEDAVYEAARPWQQAHLVLSTGER"
     gene            35496..36269
                     /gene="hisF"
                     /locus_tag="PL014_00165"
     CDS             35496..36269
                     /gene="hisF"
                     /locus_tag="PL014_00165"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOT55532.1"
                     /translation="MSVAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVALARRYGEQG
                     ADELTFLDVTASSGDRATMFDVVARTAEEVFIPLTVGGGVRTVDDVDRLLRAGADKVG
                     VNTAAIARPELIAETADRFGAQVLVLSADVRRRRDEHGMPCGGFEVTTHGGRTRTGID
                     AVQWCVRAAELGAGEILLNSMDADGTKDGFDLELIRLVRAEVTVPVIASGGAGRVEHF
                     APAVRAGADAVLAASVFHFGELTIGQVKDALRADGLPVR"
     gene            complement(36293..36946)
                     /locus_tag="PL014_00170"
     CDS             complement(36293..36946)
                     /locus_tag="PL014_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HOT55533.1"
                     /translation="MTSETHLPVVQAEREALCDTFLELGPDAPTLCDPWRARDLAAHL
                     VVRERRPDLAPGILLPALAGRLERGQRAVAEGDWESLVATVRSGPPWWSPTRIERVDA
                     LVNTVELVVHHEDVLRGDGTVGPRREVPERTERAVHAALRKSARLMFRRAPVGVRLVA
                     PGAEPIIAGPDAPVVTVTGAPVELLLLAYGRIRVASVDLDGTPADVEALRTAMLGIS"
     gene            37179..37559
                     /gene="hisI"
                     /locus_tag="PL014_00175"
     CDS             37179..37559
                     /gene="hisI"
                     /locus_tag="PL014_00175"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOT55534.1"
                     /translation="MRTPPFLDSDLDPAIAARLTRDADGLVAAIVQQHDTGEVLMLGW
                     MDDEALHRTLTQGRVTFWSRSRSEYWRKGDTSGHVQHVRSVALDCDGDALLVRVDQVG
                     AACHTGDHTCFDAADLGAVVGDGA"
     gene            37556..39139
                     /locus_tag="PL014_00180"
     CDS             37556..39139
                     /locus_tag="PL014_00180"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOT55535.1"
                     /translation="MSTASESGSADRATTPDLAYGQTWPNLDVFGVLARDRRVIPVVR
                     RLMGDAETPVGVYRKLAGDRPGTFLLESAEHGGVWSRWSIVGAASRATLTERDGAAHW
                     IGEPPVGVPTSGLATDALAETMRVLATEPIAGLPPLTGGMVGAITYDAVRRWERVPAT
                     APDELDLPELALMLATDLAVLDHADGSILLVANAINYDGTDERVEQAWADAVARLDDM
                     TRALAAPAPSTVAVVDTASVDAAMATVTSNTTRERYEEVVELAKEDIRAGEVFQVVIS
                     QRFSLDCAADPLDVYRALRASNPSPYMYLFRLPHPDGSSYAVVGSSPEALVRVTGDRA
                     ITHPIAGSRPRGKTPEDDVRLADELLADPKERSEHVMLVDLGRNDLQRVCRPGTVDVV
                     EFMDVRRYSHVMHIESTVVGDTRPGVSAYDVLVSTFPAGTLSGAPKPRALALIEQYEP
                     SRRGVYGGVVGYLDFHGDLDMAIAIRTAVVKDGVAHVQAGAGIVADSVPAREHEECVH
                     KATAALRAVATAAAMRTVT"
     gene            39136..39738
                     /locus_tag="PL014_00185"
     CDS             39136..39738
                     /locus_tag="PL014_00185"
                     /inference="COORDINATES: protein motif:HMM:NF021079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HOT55536.1"
                     /translation="MSRLSSKGVVVLLALLGAGGLLVSGFRPWVTGAVDDAVLGATRI
                     SATGAQVAPGLSAVALSIAAAAIAAVAAGRIARVVALVAYAASVVIAVILTVRVLRDP
                     EAVLGPVAASRVGRTGSIENVADPTVWPWVALAACAVALLGLVGAVRGLRVWGGPSSR
                     YEVADAGGPVAGPRGERVASAWDELSAGRDPTDVGPDQRT"
     gene            39797..40024
                     /locus_tag="PL014_00190"
     CDS             39797..40024
                     /locus_tag="PL014_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HOT55537.1"
                     /translation="MDEHEDHGHSVAAWTAVIICIIASAIMSLAVVFPNRWLFVGGVV
                     LAVVGVVAGKVLSMAGYGAAARDPEQAPSIH"
     gene            40024..40848
                     /gene="trpC"
                     /locus_tag="PL014_00195"
     CDS             40024..40848
                     /gene="trpC"
                     /locus_tag="PL014_00195"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOT55538.1"
                     /translation="MGDATAGTVLDAIIAGVREDLAVREAARPQAEVERDALAAPPAL
                     DAEEALRRPGLSLIAEVKRASPSKGELSDIPDPAALAAAYQAGGASVVSVLTEARRFG
                     GSLADLDAVRAAVTIPVLRKDFVVTDYQVWEARAHGADVVLLIVAALSDEQLTALLSR
                     VRELGMTALVEVHDEAETQRAVDAGATVIGVNARNLKTLDIHPETFSRLVPLIPPGCV
                     TVAESGIADAADAGAYARQGADAVLVGEALVRTGDPRGGAAAMVAAGAITDREAEQ"
     gene            40872..42089
                     /gene="trpB"
                     /locus_tag="PL014_00200"
     CDS             40872..42089
                     /gene="trpB"
                     /locus_tag="PL014_00200"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOT55539.1"
                     /translation="MRDLTPHELPRPGRFGEFGGRYVPEALVPALDQLDEARQKAAVD
                     PEFQAELAHLHATYTGRPSIITEVPRFAAHCGGARVILKREDLNHTGSHKINNVLGQA
                     LLTKRMGKTRVIAETGAGQHGVATATAAALLGLECTVYMGKVDTERQALNVARMRILG
                     AEVIPVEHGSATLKDAINEALRDWVTNVQSTHYLIGTVTGPHPFPEMVRDFHKIIGEE
                     ARAQVLDLTGRLPDAVIACVGGGSNAMGIFHRFIEDAGVRLIGIEAGGEGIESGRHAA
                     RFASASPGVLHGAMSYLMQDDDGQTVESHSISAGLDYPSVGPEHSFLRDSGRAEYRYA
                     TDDRVMEAFSLLSRTEGIIPAIESAHALVGAMEVGRELGPDALLLVNLSGRGDKDMET
                     AARYFGLLDGERA"
     gene            42086..42901
                     /gene="trpA"
                     /locus_tag="PL014_00205"
     CDS             42086..42901
                     /gene="trpA"
                     /locus_tag="PL014_00205"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOT55540.1"
                     /translation="MSTVVNGVGEILQRCREEGRAALVGYLPVGFPTVEGSLEAMRVL
                     VEAGCDIVEVGVPYSDPLMDGPVIQHAASTALAGGARVDDVFRATQAVRDAGGAALVM
                     TYWNLVLRRGVERFAADLASSGGAGLITPDLIPDEAGEWIAASDAHGLDRVFLVAPSS
                     TPERLSAVTGACRGFVYAASTMGVTGARTSVDSDARGLVERTRSVTDLPVCVGLGVST
                     GAQAAELAAYADGVIVGSALVRTLTGPGELCDRLEALRALTKELADGVRGGRG"
     gene            42898..43410
                     /locus_tag="PL014_00210"
     CDS             42898..43410
                     /locus_tag="PL014_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOT55541.1"
                     /translation="MSTATDPTLHEVSPTPERGERLKDVIGLLARLFLGAVLIYAGAV
                     KVGKPLTSERAVQAFEIFPTDLAGYIGLALPFVEILLGALLILGLFTRPVAIVSTLLM
                     VAFIIGIAQAWARGLTIDCGCFGGGGQVAAGETKYGTDIARDATFALAGAWLWWRPRS
                     LASLDRLLFR"
     gene            43432..44241
                     /locus_tag="PL014_00215"
     CDS             43432..44241
                     /locus_tag="PL014_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT55542.1"
                     /translation="MAKNSAKAPDRGAANARAAKIQAAQKSAGGGANKIVVATVVAIV
                     AIIATVGGVVWSQMSKENDVTGGGNALPAGVSELGEGYPAFQDVTPVDGAPTVDLYVD
                     YQCPACAMFEQAFGPTLSEQAADGKIKLVYHVKNFLDDNLRNDSSTRSANGAFCASDA
                     GKFQEYSDQLFPGQPEREGDGWTDAQLKAFAETAGLSGADLTTWESCVAAGKYTDYVN
                     SVEKQSFADGVKGTPTVKVNGEDLDTGTLVNAEGTAFDPAKLVAALEAAGK"
     gene            44238..45104
                     /gene="lgt"
                     /locus_tag="PL014_00220"
     CDS             44238..45104
                     /gene="lgt"
                     /locus_tag="PL014_00220"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT55543.1"
                     /translation="MIASLPSPTVGVWHLGPFPLRMYAVCILLGIVVAVWLTGRRLVD
                     RGYEAGQALDVAAYAVPFGIVGGRIYHVITTPDPYWGEGGNPVDALKIWEGGLGIWGA
                     IALGAFGIWVGCRQVGIRFLPFLDAAVPGVALAQAIGRVGNWFNNELYGGPTTLPWGL
                     TIHQWDQGAGRAVEDASGNPVVLGVFHPTFLYEMIWLVILAVALLVIDKRRALAPGQL
                     AGLYVAGYPVGRIVIEFMRTDKAELILGVRLNVWTSIIVFALGVWIYWFTGRRAERNA
                     VPQSEPESRNVG"
     gene            45232..49773
                     /gene="gltB"
                     /locus_tag="PL014_00225"
     CDS             45232..49773
                     /gene="gltB"
                     /locus_tag="PL014_00225"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOT55544.1"
                     /translation="MTVARFSAQPTDTGLYRREHEHDACGVALVATMRGSAGHDIVAH
                     ALDALRNLDHRGATGADPLVGDGAGILTQVPDAFFRAVLDADLPEAGAYAVGMAYLPQ
                     DDDERALAEQRIADIAVDEGLRVIAWRDVPVTPDLVGQSARECMPVFRQLFVTSEQGL
                     RGIELDRVAFCLRKRAEHEAEVYFPSLSSRTVVYKGMLTTGQLEPFFPDLSDERFASE
                     LALVHSRFSTNTFPSWPLAHPYRLIAHNGEINTVKGNRNWMRARESQISSTVIPGDIE
                     RVFPICTPGASDSATFDEVLELLHLGGRSLPHAVLMMIPEAWENHESMDPARRAFYEF
                     HSTFMEPWDGPACVTFTDGTLIGAVLDRNGLRPGRYWVTDDGLVVLASEAGVLDIEPE
                     RVVRRGRLQPGRMFLVDTAAGRIISDEEVKSGLAGEHPYEEWLHAGLLELSDLPEREH
                     IIHTAASVARRQRTFGYTEEELKLILAPMASAGLEALGSMGTDTPLAVLSDRPRLVFD
                     YFSQLFAQVTNPPLDAIREELVTSLGSSIGPEGNVLDASPAHARQVVLPFPVIDNDEL
                     AKLVHINADGDLPGYSTVVVKGTYDVNGGAAALRARLHEIFAEVSTAIGQGARFVVLS
                     DRDSDRDLAPIPSLLLTSAVHHHLIREKTRTQVGLLVEAGDVREVHHVALLVGFGAAA
                     INPYLAMETVEDMVRSGAITAVTEEKAVANLIKALGKGVLKVMSKMGISTVASYRGAQ
                     VFEAIGLSQDLVDEFFTGTVSQLGGVDLDVIAAETAARHAMAYPPDGIRQAHRKLLVG
                     GEYQWRREGEPHLFDPETVFRLQHATRARRYDVFKQYTRRIDEQAGRLMTLRGLFELG
                     GAQPRPPVPIEEVESVAEIVKRFNTGAMSYGSISMEAHETLAIAMNRLKARSNTGEGG
                     EDPERLMDPQRRSAIKQVASGRFGVTSLYLTHADDIQIKMAQGAKPGEGGQLPGPKVY
                     PWIAKTRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPQARIHVKLVSEVGVGT
                     VAAGVSKAHADVVLISGHDGGTGASPLTSLKHAGGPWELGLAETQQTLVLNGLRDRIV
                     VQVDGQIKTGRDVVIAALLGAEEFGFATAPLVVSGCILMRVCHLDTCPVGIATQNPEL
                     RARFSGQPEFVETFFEYIAEEVREHLAALGLRSIEEAIGQVQYIDASKAINHWKASGL
                     DLAPILARVESPDGSGIRHTIGQDHGLEKALDHELIAASRDAIDHGTPVTARFRIRNV
                     NRTVGTMLGHEVTRAHPNGLPDGTIDLTLTGSAGQSLGAFLPAGITLRLEGDANDYVG
                     KGLSGGRVIVRPAPASALVAEDNVIGGNVIGYGATSGELFLRGRVGERFCVRNSGATA
                     VVEGVGDHGLEYMTGGTALILGPTGRNVAAGMSGGVAYVLDLDPAHVNRELVDLGPLR
                     AEDELVVHDLLERHHRWTDSAVAARLLADWDSARSAFTLVLPRSYQRVLDVRAKAAAE
                     GLDPDGTQVWERIMEASRG"
     gene            49766..51223
                     /locus_tag="PL014_00230"
     CDS             49766..51223
                     /locus_tag="PL014_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOT55545.1"
                     /translation="MADPQGFLRTRERELPRRRPVPVRIKDWKEVYEEQPVPELQRQA
                     GRCMDCGIPFCHSGCPLGNLIPEWNDLAWKGEWRQAIDRLHATNNFPEFTGRLCPAPC
                     EAACVLGINQPAVTIKQVEVSIIDRAFENGTVTPEAPERLSGKTVAVIGSGPAGLAAA
                     QQLTRAGHTVAVYERADAPGGLLRYGIPEFKMEKAVLDRRIAQMKSEGTRFRNGVEIG
                     KDLTAADLRKRFDAVVIATGATVPRDLPVPGRELDGVMQAMDFLPPANRVARGQHVDG
                     QVTAEGKDVIVIGGGDTGADCIGTSHRQGARSVTSLEIMPQPPGERPGNQPWPTYPMT
                     FRVASAHEEGGDRVYAVSTSEIVGDEHGRVAALRLHEVRMGENGFEKVEGTERELPAQ
                     LVLLAMGFLGPQQDLLDQFGVERDARTNAARDDAYMTNVPGVFVAGDAGRGQSLIVWA
                     IAEGRSAANGVDAWLMGKPSRLPRPVNPTDRPLMA"
     gene            51325..52770
                     /gene="pyk"
                     /locus_tag="PL014_00235"
     CDS             51325..52770
                     /gene="pyk"
                     /locus_tag="PL014_00235"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOT55546.1"
                     /translation="MRRAKIVCTLGPATSSAENLRELVRSGMDVARLNLSHGDHTDHE
                     RVYHDVRRASDAMGRAVGILVDLQGPKIRTARFENGPIELVRGASFTITTRDVPGNEV
                     EVGTTYAGLPGDVAIGDRILIDDGKLALIATEVTDTDVVCQVIEGGMLSNNKGINLPG
                     VAVSVPAMSEKDKDDLRWALRMRVDMIALSFVRSAEDILDVHKIMDEEGLRLPVIAKI
                     EKPQAVENLDEIIRAFDGVMVARGDLGVEMPLEEVPLVQKRACEIARRRAKPVIVATQ
                     VLESMIDNARPTRAEASDAANAVLDGADALMLSGETSVGKHAFAAVRTMARIIENTED
                     HGLHRIRPLGTRPSSKGGAVTAAAADIGELLGVKFLITFTESGDSTRRLSRVRPRIPM
                     LAFTPNQWTRSQLALTWGVETFLVGKAKHTDEMAMQVDEVLLAAGRVLEGDTVIIVAG
                     SPPGIPGSTNALRVHRVGDAKNRVAPAYSEL"
     gene            52825..52907
                     /locus_tag="PL014_00240"
     tRNA            52825..52907
                     /locus_tag="PL014_00240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52858..52860,aa:Leu,seq:caa)
     gene            52909..53535
                     /locus_tag="PL014_00245"
     CDS             52909..53535
                     /locus_tag="PL014_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT55547.1"
                     /translation="MSETSPVPVAPAAAPSGPTKRVLVAEDEAIIRLDLAEMLTEAGY
                     EVVGQASDGEQAVALAASLKPDIVIMDVKMPVMDGISAAEQIGKERLCPVVMLTAFSQ
                     TELVERARDAGVMAYIVKPFTASDVTPALDIALSRWAELKTLEAEVADLGDRLETRKA
                     VDRAKGVLMTKLKITEADAFRWIQKTAMDRRMSMREVADAVVAGMEKA"
     gene            complement(53549..54331)
                     /locus_tag="PL014_00250"
     CDS             complement(53549..54331)
                     /locus_tag="PL014_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOT55548.1"
                     /translation="MTSLFPGAGTALNVLTVLIGAGLGMAVGHRLREHTRSVVTDVLG
                     LVTLLVAGLSAMAVTDVALSDAVGSGFPVLIVLGSLLIGSITGSMLRIEERLEGLAGT
                     IQGWVSRRSKDSSIEGGSDHASRERFIEGWLTASLLFCVGPLTILGALNDGLGRGIDQ
                     LALKAVLDGFAALAFAASFGVGVLLSAVSVLVVQGALTVVGVLLGDLVPDAHISALTA
                     TGGLMLAGIALRLLRIRDIPVGDMLPALIVAPLLTQLVIWLR"
     gene            54497..55042
                     /locus_tag="PL014_00255"
     CDS             54497..55042
                     /locus_tag="PL014_00255"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT55549.1"
                     /translation="MPVDRDDLRDVMGLWLAARVDSGSSKEAAGRALTDGRLSTALRR
                     PGVHSFVARMDGEPVGYAIASENPFGLSPQAELAIEQLWVGPDARRHGVAKSLLAAVL
                     STAERSGCEVVVSNVPTASRDANRFFARLGFASVITRRVVSTSALRRKLAPESVETGH
                     ELLRRRRSLRSRAFPAASRQF"
     gene            complement(55051..55443)
                     /locus_tag="PL014_00260"
     CDS             complement(55051..55443)
                     /locus_tag="PL014_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOT55550.1"
                     /translation="MGAMNPNSLMHRMGIEIIEASAQRLVATMPVEGNTQPYGLLHGG
                     ASVVLAETLGSIGAALHAGPGKAVVGLDINATHHRAARSGLVTGVATPISLGRTLACY
                     EIVVTDDTGARTCTSRITCLIRDAAPGA"
     gene            55526..58219
                     /gene="polA"
                     /locus_tag="PL014_00265"
     CDS             55526..58219
                     /gene="polA"
                     /locus_tag="PL014_00265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOT55551.1"
                     /translation="MVDRLPRVKLLLLDGHSLAYRAFFALPAENFSTSTGQHTNAVYG
                     FTSMLINVLRDEAPTHVGVAFDVSRKTFRAAEYPEYKAGRSATPTEFSGQVPLLHEVL
                     DALRIRHVGIEGYEADDVIATLTHRAVEQGHEVVICTGDRDAFQLVSDKVTLLYPVRG
                     VSEVWRMDPAAVTEKYLVPPERYSDLAALVGESSDNLPGVPGVGPKTAAKWLGQYGDL
                     AGIVAHVDEIKGKAGENLRAHLDGVLRNRRLNKLVDDLDLPLGIDDLQRASWDREEVH
                     RVFDGLEFRVLRDRLFATFDVEADEVEGGFELEGEVVHADGLALRFGEGAHGRQVPHG
                     VVVTGHYRSGSGDAGAVAFAGADGVTGYVDLTTLTSEQDEALASWLADPSAPKVLHDA
                     KEQLQALSTRGLTLAGITSDTALAAYLVRPDQRSYDLEDLVVRHLGRELKAQEAAGEQ
                     GMLDFAPDDDTSEREAMVRARAVLELSEVLGERLEETGGTDLLRDLELPLVDILARME
                     RTGIAVDEDALTALEADFAAKMRQAQLDAYEAIGRSDVNLGSPKQLQEVLFDQLGMPK
                     TKRTKTGYTTDADALADLFAKTEHPFLEALLRHRDTSRLRVTVEGLIKSVAPDRRIHT
                     TYIQTIAATGRLSSTDPNLQNVPIRTEEGRRIREVFVVGEGYESLMSADYSQIEMRIM
                     AHLSGDEGLIEAFRTGEDLHRFVGARVFGVEPQDVTAAMRSKVKAMSYGLAYGLSAFG
                     LSKQLNISTGEAQGLMDEYFSRFGGVRDYLREVVVEARATGYTATMLGRRRYLPDLVS
                     DNRQRREGAERMALNAPIQGSAADIIKLAMKGVDAALRTEGLASRILLQVHDELVLEV
                     APGEREALESLVRREMGAAVAMDVPLDVSVGVGRSWHDAAH"
BASE COUNT         8053 a        21680 c        20664 g         8041 t
ORIGIN      
        1 cgacgatcat gaccgacccc gagttcgccg acgcgaccta cgtcgagccg atcaccccgg
       61 agatcgtcga gaagatcatc gccaaggagc gaccggatgc cgtgctcgcc accctgggtg
      121 ggcagaccgc gctgaactgc gcgatcgagc tgcacgagcg cggtgtcctc gagaagtacg
      181 acgcgcccct catcggggcc aacgtcgagg cgatcgagct cggggagaac cgggagaagt
      241 tcaagggcgt ggtggagcgc tgcggcgccg agtcggcccg ctcggtcatc tgccacacca
      301 tgggtgaggt gctcgccgcg gccgacgacc tcggctaccc ggtcgtcgtc cgaccctcgt
      361 tcaccatggg gggcctcggc agcgggttcg cccacgacga ggacgagctc gtgcgcatcg
      421 ccggtgcggg cctgcagtac agcccggtca ccgaggtgct cctcgaggag tcgatcctgg
      481 gctggaagga gtacgagctc gaggtgatgc gcgaccacgc cgacaacgtc gtcgtcgtct
      541 gctccatcga gaacttcgac ccgatgggcg tgcacaccgg cgactcgatc accgtggccc
      601 ccgcgctgac cctcaccgac cgcgagtacc agcgcctgcg cgacgtcggc atcgcgatca
      661 tccgcgaggt cggggtcgac accggcggct gcaacatcca gttcgcgatc aacccggccg
      721 acgggcgcct catcgtcatc gagatgaacc cccgcgtctc tcggagctcg gcgctggcgt
      781 ccaaggccac cggcttcccg atcgcgaaga tcgcggcgaa gatggccatc ggctacaccc
      841 tcgacgaggt gcgcaacgac atcacgcgag agaccccggc atccttcgaa ccgacgctcg
      901 actacgtcgt cgtcaaggtg ccgcggttcg cgttcgagaa gttccccgcg gccgacgaca
      961 cgctgacgac gacgatgaag tcggtcggtg aggcgatggc catcggacgc aacttcaccg
     1021 aggcgctgca gaaggcgctg cggtccctgg agaagaaggg gagcagcttc cactgggacg
     1081 gcgaacgccc cacgaccgag caggcccgag agatcctcgc cgtggccacc cgcccgaccg
     1141 acggccgcat catcttcgtc cagcaggcac tgcgcggtgg gctctcggtc gaggaggtcc
     1201 acgaggccac gggcatcgac ccgtggttcc tggaccagat ggccctcatc gagcagatcg
     1261 cggatgccgt cgcagcagcc ccggaactga cccccgagct gctgcggctg gccaagaccc
     1321 acgggttcag cgacgcccag atcgccgggc tgcgtgccat gcccgaggcg gtggtgcgcg
     1381 gagtgcgcca cgccctgggg gtgcgccccg tcttcaagac ggtcgacacc tgcgccgccg
     1441 agttcgcggc ccagacgccc taccactaca gcacctacga cgaggagaac gaggtcgccc
     1501 cccgtgagcg gcccgccgtg ctgatcctcg ggtcaggtcc caaccggatc gggcagggcg
     1561 tggagttcga ctactcctgc gtgcacgcca gcttcgccct gcgcgacgcc ggctacgaca
     1621 ccgtcatggt caactgcaac cccgagacgg tgtccaccga ctacgacacg agcagccgtc
     1681 tgtacttcga gccgctcacc ctcgaggacg tcctcgaggt cgtccacgcc gagcggcacg
     1741 ccggcccgat cgccggggtc atcgtccagc tcggtggcca gaccccgctc ggcctggcca
     1801 aggcgctcaa ggccgagggg gtgccgatcg tcggcacgtc acccgaggcc atcgacctcg
     1861 ccgaggaccg tggcgcgttc ggccgcgtgc tgcacgaggc gaacctgccc gcgcccaagc
     1921 acggcacggc ctacagcgcc cacgaggccg tcgacatcgc ccgggagatc ggctacccgg
     1981 tgctcgtgcg tccgtcgtac gtgctcggcg gtcgcggcat ggagatcgtc ttcgacgacg
     2041 agacgctctc cggctacgtc gagcgggcca ccatcgccag cccggagcac cccgtgctcg
     2101 tcgaccgctt cctcgacgac gcgatcgaga tcgacgtcga cgtgctctac gacggggtcg
     2161 acatctatgt cggcgggatc atggagcaca tcgaggaggc cggcatccac tccggcgact
     2221 cctcgtgcac gctgccgccg gtcaccctcg gcgacagcga gctcgagcgg gtgcgggtct
     2281 cgacccgcaa gctcgccgag ggcatcgggg tgcggggtct gatgaacgtg cagttcgcgc
     2341 tcgcgcagga catcctctac gtcctcgagg ccaacccgcg cgcgtcgcgg accgtgccgt
     2401 tcgtcgccaa ggcgaccggg gtgccgatcg cgaaggcggc cgcccggatc atgctcggcg
     2461 cgaccatcgc cgagctgcgt gacgaggggc tgctgccggc gcacaccgac ggtggtcgga
     2521 tgccggccca cgcgccgatg tcggtcaagg aggccgtgct gcccttcaag cgcttccgca
     2581 ccgccgacgg gctcgtcgtc gacagcctgc tgggccccga gatgcgctcc accggtgagg
     2641 tcatgggcat cgacaccgac ttcggccggg ccttcgccaa ggcccagctc ggtgcgatca
     2701 gcgggctgcc cacgacggga acggtgttcg tatcggtggc caaccgcgac aagcgggcca
     2761 tcatcttccc ggtcaagcgc ctgctggacc tcggcttccg gatcgtgtcg acgtcgggca
     2821 ccgctgacgt gctgcgccgc aacggcatcg aggccgaggt cgtgctcaag cacgccgagc
     2881 gcggccccga ggggcgctcg atcgtcgact ggatcaacgc cggtgaggtc gacatggtgg
     2941 tcaacagccc cagcgggccg agcgcgtccg cccgggccga cggctacgcc atccgggcgg
     3001 ccacgaccgc catggacaaa ccgatcatca cgacggtgca gcagctggca gctgcggtgc
     3061 aggcgatcga ggccgtgatc accggcgacc tcgacgtcac cccgttgcag cagcacgccg
     3121 ccgacctcga cctctacggc gtgcaggcac cggcgtgagc gcggccagcg tcgccccggc
     3181 ggcgggtggg gtcacccagg tcgtcggcag cgtcgtcgcc aacgaccccg tcggtgccta
     3241 ccgccacctg gtgatctcgg cccccggggt ggcggagctc gcctctcccg ggcagttcgt
     3301 cgccctcgcg gtcggcgggc cgacctcggc caacctgctg cgccggtgct tctccctgca
     3361 tcgggtggat gtcggcctcg gcaccatcga ggtggtcatc gccgcgcacg ggcccggaac
     3421 gacgtggctg accgaggtgc gcgttggtga ccccgtcgac gtcgtggccc ccctggggcg
     3481 accgttcccc ctgcccgagc agcctgaggc ctgcgtgctc gtcgggggtg gctacggctc
     3541 cgcgccgctc ttctggctcg ccgaggcttt gcgcacccgt gccggccacg tcgagatggt
     3601 gctcggcgcg gcatccgagg accggatctt cggggcctcg ctggcccgcc gagatgccga
     3661 cggggtcacc gtgaccaccg acgacggctc gatcggccgc cgcggctggg tgtccgtcgt
     3721 gctcggtgac gtcatcgacc gtaccaacgc cacggtcgtg tacgcctgcg gcccgatggc
     3781 catgctgaag tcggtcaccg acatcgccac cgcccacggg gccgtggccc acgtggccgt
     3841 cgaggagtcg atggcctgcg gcatcggggt gtgcatgacc tgcgtgctgc cgatcacggg
     3901 tgaggacggc gtgacgaaga tggtgcgctc gtgcgtcgac ggcccggtgt tccggggtga
     3961 ccgcgtgcgc tgggacgcct tcggcgacgg catctgccgg gtgccggccg acaccctcgg
     4021 cgcgccgcag gcggggggcc actgatggtg gacatgagca ccgcgctcgg tgcgctcacc
     4081 ctgcccaacc cggtcatgac ggcatccggc tgcgccgcga acggccagga gctgaaccgc
     4141 ttcttcccgg tgtccgagct cggggccttc gtcaccaaga cggtcatggc cgcgccgcgc
     4201 tcggggcgag gcaccccgcg gatggccgag acggcatccg gcatgctcaa ctcgatcggg
     4261 ctccaggggc ccggcatcga ggcattcgtc gagaacgacc tcgcctggct ggcctcggtg
     4321 ggcgctcggg tgctcgtgtc gatcgccggc aacaccgccg cggagttcgg ggaggtcgcc
     4381 gcctgcctcg ccgacagccc ggcctttgcg gccgtcgtcg gggtcgaggt caacatctcg
     4441 tgccccaacg tcgccaaccg cggcctggtg ttcgcgtgcg accccgtctc gtccgacgcg
     4501 gtggtgcgac tcgttcgcga caagctcccg gcggcgacgc cgatcttcgc caagctcacc
     4561 ccggacgtca ccgacatcgt gaccatcgcg gatgccgcgc tcggcgccgg ggccgacggc
     4621 ctgacgatga tcaacaccct gctcggcatg gtcatcgaca ccgacctgct gcgaccacag
     4681 ctggctgggg tcaccggtgg gctgtccggc ccggccgtgc gccccgtcgc cgtgcgcgcc
     4741 ctgtggcagg tcacggcggc gatgcgcgac gggcgcctgc ccaccgcacc cctcatcggg
     4801 gtcgggggag tgcgcaccgg gcgcgatgcg ctcgagctcg tcgccgccgg tgccagcgcc
     4861 gtgcaggtgg gcaccgcgac gttcaacgac ccctcgcgcc cgagccgggt cgtcgacgag
     4921 ctcgaggccg aggtcgcccg ccacgggttc acccgcttcg ccgacgtcgt cggcatcgcc
     4981 cacgacagga tggtttcgcc atgagtctcg acccgttcgg cgtgcggttg cgcgccgcca
     5041 tggacgcgca cggcccgctg tgcgtcggca tcgacccgca cgccgcgctg ctccaggcgt
     5101 gggggctgcc ggtcgatctg tccgggctcg agcggttcgc gatgacgtgc gtcgaggcct
     5161 tcgcggggcg cgtcgcggtc gtcaagcccc agtcggcgtt cttcgaggtg ttcggctcgc
     5221 gcggtgtggc ggtgctcgag cgggtgctcg ctgacctgcg cgacgccggg accctgacga
     5281 tcctggacgc caagcgtggc gacatcggct cgaccatggc ggcgtacgcg caggcattcc
     5341 tctccgacga ggggccgggt cgtgccgatg ccctcaccgt gagtcccttc ctcggctacg
     5401 ggtcgctgcg cccggccctg gacgtcgccg ccgccaccgg gcgcggggtg ttcgtcctcg
     5461 cgctgacgtc gaaccccgag ggtccccagg ttcagcacgc gcagacgccg cagggctcgg
     5521 tcgccggcgc ggtggtcgcc ggtgctgctg ccgacaacgc cggggctgcc gcacggggcg
     5581 agatgggcca cgtcggcctc gtcgtcggcg cgaccgtcgg ggatgccgtg acgcgcctcg
     5641 gtctcgacct cgcctccagc gccgcccccc tcctcgcgcc ggggctcggt gcgcagggtg
     5701 ccggccccgg tgacctgcgc gcggtcttcg gtgacgccgt gggcaacgtg ctgccgaacg
     5761 tgtcgcgctc ggtgctctcc gcagggccgt cggtgcaggc gctcgccgcc gccgcggcac
     5821 gtgacgccga cagcgtacgt gctgccctgt gagccagcgc tagagtcgaa ccactccccg
     5881 tcgggtgctg atgcagatgg ccccgcatgt cgccgaagga cgcacctgtg gcacttccgc
     5941 cgctcacccc cgaacagcgt tccgccgccc tggccaaggc ggcggcgtcc cggcgtgagc
     6001 gcgccgaggt caagcaccgc ctcaagcact cgggcctctc gctcgccgag gtgatcgacg
     6061 ccggcaagac caacgaggtc gtcggcaaga tgcgcgtctc cgccctgctg gagtcgctgc
     6121 ccggcgtcgg ccgggtccgc gcacgacaga tcatgcagga ggtcggcatc tccgagagcc
     6181 gccgcgtgcg cggcctcggc cagaaccagg ccgccgccct gatcgagcgc ttcggacggt
     6241 gagtgccccc tcgcgcctga cggtcctcgc gggacccacc gcggtcggca agggcacggt
     6301 ggcggcctac gtgcgcgaac accaccccga ggtctgggtc tcggtgtccg cgacgacccg
     6361 ccgcccgcga cccggcgaga tcgacggcat ccactaccac ttcgtcgacg acgagcactt
     6421 cgacgcgatg gtgcgcgacg gcgagctcct cgagtgggcg gtggtgcacg gccgcgcgaa
     6481 gtacggcacc ccccggggcc cggtcgagca ggccctggcg gccgggaggc cggccctgct
     6541 cgagatcgac ctccaaggcg cccgtcaggt tcgcgagacg atgcccgagg cgttgttcgt
     6601 cttcctcgcg ccgccgacgt gggacgaact cgtgcgacgg ctcgtcggcc gggggaccga
     6661 gggtgaggag gaacgcgagg cccgactgtc caccgcgagg gtcgaactgg cctccgcgag
     6721 cgagttcgat gtcaccgtcc tcaacgacga cgtccaccgg gcggccgagg aactcgtatc
     6781 attgatgaga acccctccca cctgagcgaa gcgagatcca gcgtgtccgg aaccgtggcc
     6841 gaccccatcg gcatcaccaa ccccccgatc gacgagctcc tcgagcgcgc cgactccaag
     6901 tactccctgg tgctctactc cgcgaagcgg gcccggcaga tcaacgccta ctacgcccag
     6961 ctccaggagg gcctgctcga gtacgtcggg ccgctcgtcg actccgaggt gcacgagaag
     7021 cccatgtcga tcgcccttcg cgagatcaac gagggcctgc tgacctcgac ccccacgccg
     7081 tcccagggct gacctgacgt gggcgccggg gtggatgcgt gaaggtcgtc ctcggcgttg
     7141 ccggtggcat cgccgcctac aaggccgcgt cactgctccg gctgttcacc gaggccgggc
     7201 acgacgtcac ggtggtgccg acgcaggctg cgctcaactt cgtcggcgcg gccacgtggt
     7261 cggcgctgtc cggcaagccg gtgcacaccg acgtgtggtc cgacatcacg gcggtgccgc
     7321 acgtgcgcat cggccaggag gccgacctcg tcgtcgtggc gcccgccacc gctgacctgc
     7381 tcgcccgcgc ggccaccggg caggcgaacg acctgctcac gacggtgctg ctcacggcgc
     7441 ggtgcccggt ggtgatggcg cccgccatgc acaccgagat gtgggagcac gccgccaccc
     7501 aggccaacgt cgcgacgctc agcgagcgcg gggtgcacat cgtccccccg gcatccggcc
     7561 gcctcacggg cagcgacacc gggcccggcc gcctgcccga gcccgaggac ctgttcgccg
     7621 tctgcgcccg catcgtcccg accacgcctg ccggtgaccg ggcaccacac cgggccgacg
     7681 ccgttccgga agctgccggc aggaagggtg aggacctcac gggccttcga gtcgtcgtga
     7741 gcgccggggg cacccgcgag ccgctcgacc ccgtgcgcta cctcggcaac cgctcgtcgg
     7801 gcaagcaggg gtacgccctc gcgcaggctg cggccgaccg cggggccgac gtcaccctgg
     7861 tgtcgtcgtc gtcacagccc gtgccggccg gcgtcacgct cgttcccgtc gagaccgccc
     7921 tggagctgca ggccgccgtc acgcacgccg tgcacggggc cgacgtcgtc gtgatggctg
     7981 ccgccgtggc cgacttccgc ccgtcgagct atgccgacca caagatcaag aagtcacacg
     8041 aggccgcggc agacggcagc gccgacgagt cggccccggt cgtgaccctg gtgcgcaacc
     8101 ccgacatcct cgccgggctc gtcgaggccc gggggagtgc caccaccccc gtcatcgtgg
     8161 ggtttgccgc cgagaccggc gacggcgagg cctccgtgct cgagcacgga cgggcaaagc
     8221 tggctcgcaa gggctgtgac ctgctcgtcg tcaacgaggt gggggtcggc gtcaccttcg
     8281 ggcaggacga caacaccgtg cacatcctcg agcgcggcac cgaccacgtc accgacatcg
     8341 gtccggccac caagctcgag gtcgcgcacg cggtctggga cgccgtcacc gacctcctct
     8401 gacgcacgtg gtgcactcca gccaccgcga agcaggtggc tggagtgcac caaggtgcgg
     8461 gtgggtgggg tcagcggcgg gctgaggtct tggccttgcg cgcgctcccc ggcacgcggt
     8521 ccgactcgtc cgaaccctga tcgtccgccg tcgcgtcgtc ttccgcggtc tcggatgccg
     8581 cctgctcccc gtcgacctcc ccggtctcgg atgccgggtg gtcgccgtcg gcctcggcgg
     8641 tcgcctcgct cgtgtccacg gactcagctg ccggggcctg ctcggcgtcc tctgccgggg
     8701 cctgctcggc gggagcgtcg gcatcagccg cagcatccag ggcagtgacc gcggcgggac
     8761 caccgagggt ggcgagcatg ttgcgcacgt tgctcagctg ggcggtgatg ctgtcgcggc
     8821 gagccgtggc ggcgctcagc tcacgctcgg agtcgcggcg cactcgctct gcctgctcct
     8881 tggccgacgc gacgagggca gctgcctccg agcgggcggc gtcgagcacg cgtgcggcct
     8941 cctcggctgc cgcggcgcgg tcctgctcgg cctcgcgagc cagcaggtcg gcgcgctcct
     9001 ggacggcggc cagggcctgg tcctgcttgg ccatctggcc ggtgaactca gcggtggcct
     9061 tctcccgccg ctcggcaagg gtgcgctcga agtccgcggc ggaagcggct gcggtggccc
     9121 gctgcttctc gaagtaggcc tcggcctcct cgcggcgggc ggcggcctcg cgggcggcgt
     9181 cgtcgacgat cgagtcggcc tcctgcttgg cacgggccag ggcctgctcg gcatcgacgt
     9241 cggcgcgcga gcgtcggtcc tcggcatacc ggtcggcttc ggtccgcgcc agcgcggcgg
     9301 cctcgtcagc ggcgaaccgg gtctggccgg cctcctcctc ggcggtggcg cgaatggcgg
     9361 cggcctcctc gtcagccagc gtcaggatcg agccgatccg ctcgccgagg tctgcgaacg
     9421 acgggttcga tgcccggcga gcgtgctcct cgagctctgc cacactgcgc cgctgcccgt
     9481 cgacctcctg catgagcgcg tcgtgggact ggcgcagctt ggtcagcgag accgacgccg
     9541 aggcggcctc ctgctgggcg gcctcagccg cctgccgggc cgcctcagcg gcctggcgca
     9601 gcgcactgac gtgctggtcg accgacacgg ggtcgtaccc gcggaagacg gtggggaact
     9661 cgggctggtt catcaggact cctggtgggg cggggtttgc gggtgcggag ggtcttcggg
     9721 aacggccagg gcgtcgatga cccctgagag gctgccgagc tgggcggtga tggcgtcacg
     9781 gcgggccgcc agggtgtgca cctcggcgcg ggcccgctcg acggccgcct gggcctcggc
     9841 tcgaacggcc tcggcccgtg cgcgggcgtc ctcggaggtc cgcaggatct cggcacgggc
     9901 ggcccggtgg tgctcgtggg cttcgcggtg catgcgctcg acgtccgcag cggcccgttc
     9961 ccgaaccagg gcggcaccct gttcggcctc ggtcgttcga cgggtcgctc gctcgagagc
    10021 ctcctcggca cgctgttgcg cctcgtcgac gacccgctgg gactcgcggg cggccgcctc
    10081 acgggtgcgg gcggcatccg cctgcgcctc ggcgagcacg gcctcggcct gttcgcgcag
    10141 acgtcgtgcc tcggagtccg cctcggacag gcggtcggct cccttggccg tcgccgcggc
    10201 gagcagggtc tgcacctgct gtcgatgggc gcgggagcga tctgcgagct cgcggtggtg
    10261 ggtgcgggcg cgctgctcgg cgtcggcgcg cgctccggtc acgagctcgc gcacggtggc
    10321 ctgcgcccag gccaggtgcg cctgcgcggc tgccatgacc gagtcggcct cggcacgagc
    10381 gtcggcaagc agttcatcgg cgtgggcctt cgccccgctc accagcgact ccgccgctgc
    10441 gcgggacgcc tcgtcacgct cgccgatcgc cgccagcacc tcagcggccc gggcctgcgc
    10501 ctctccccgg tgcgtgagcg agaactgctc cgcctcgcga cgcagaccgt cgagctccgc
    10561 cagcgtgctc tcgcgcaggg cgtgcagctc ggtggtgtgg gcggtgtgct cactgctcca
    10621 ccgctgctgg tgggcacgct cgtcgtcggc cagacgagcg accgtcagct cgtgctcgcg
    10681 acgggcctgc tcgaggatcg cctcggcggc ggtccgagcc tgcgccagct cggcctgggc
    10741 ctgctcggtc gcgaggccca gggcctcatc gctggccttg gtcgcccggg ctcgcagcag
    10801 cgcagcctgg tcggcggcgt cggcgagcat gatctcgacc tcggcgcggg tctcctcgag
    10861 ccggcgggcc gcctcggccc gggcggcggc aatcccggcg cgcaggtcgt ccagctcgtg
    10921 accggcctcc tgcgcctcgg cgcgaaggcg agtggcttcg tcgcgggcgg agacgacctc
    10981 gcggtgggcc tccagccgca ggtcgtcggc cagggcctgt gcgcgggtca gcagggacag
    11041 cgcttccgcg gcggggtccg cggaggccgc acctcctgcg tccccagccg cctcggggtt
    11101 caccgaccgg tctgacacgc cgcccatggt ctcatccccc gagcggccac gtgaccggga
    11161 gtggggggaa cgacagcccg ccggtagaat cccgggggca gtcgctcgag ccgctgccac
    11221 ccccccactt gaaggagacc tgcgtgtcgg cacgcttgtt cacgtccgaa tccgtgaccg
    11281 agggtcaccc ggacaagatc tgcgaccaga tctccgactc gatcctcgac gccatgctgg
    11341 cccaggaccc cggcagccgc gtggccgtcg agacgatggt gacgaccggt ctcgtgcacg
    11401 tcgccggaga ggtgaccacc gaggcctacg tcgagatccc gaagatcgtc cgcgacacgg
    11461 tgctcgcgat cggctacgac agctcgacga agggcttcga cggggcctcg tgcggggtca
    11521 gcgtctccat cgggcagcag agccccgaca tcgcccaggg tgtcgacacc gccttcgaga
    11581 gccgcaccgg cgccgtcgac ccgctcgaca agcagggcgc tggcgaccag ggcctgatgt
    11641 tcggctacgc ctgcgacgac acccccgagc tgatgccgtt gccgatcttc ctggcccacc
    11701 gcctcgccga gcgcctcacg gcggtgcgca agaacggtga gctgtcctac ctgcgccccg
    11761 acggcaagac ccaggtgacg atcgcgtacg acggtgaccg cgcggtctcc ctcgacgccg
    11821 tcgtcgtgtc cagccagcac gccgaggaca tctccctcga gggcatgctc gagcccgacg
    11881 tgcgggcgca cgtcatcgac ccggtgctcg ccgagctcgc cgactccggc accgagctgc
    11941 acaccgacga ctaccgtgcg tacatcaacc ccaccggcaa gttcgtcatc ggtggcccga
    12001 tgggtgatgc cggcctgacc ggccgcaaga tcatcgtcga cacctacggc ggcatggccc
    12061 gtcacggtgg gggcgccttc tccggcaagg acccgagcaa ggtcgaccgc tccgcggcgt
    12121 acgccacgcg atgggtcgcc aagaacgtcg tcgccgccgg gctggcccgt cgctgcgagg
    12181 tgcaggtcgc ctacgcgatc ggcaaggcgc agccggtcgg tctctacgtc gagaccttcg
    12241 gcacggagac cgtcgccgcc gacaagatcc aggccgcgat cctcgcgacg ttcgacctgc
    12301 gcccggcggc catcctcgac gcactcgacc tcctgcgtcc gatctacaag tcgacggcgg
    12361 cctacgggca cttcggccgc accaccgcgg ccggtgtcga caaccccttc acgtgggagc
    12421 gcaccgaccg cgtcgaggcg ctccagcgcg ccgccaacgc ctgacgcggc cgacccgtcg
    12481 tccacaggcg gagcccaccg atccccgcgg ggtcggtggg ctccgctagt ttcggcgcat
    12541 ggcagtgacc ggggatccga cgagcgaggt gctcccggtg gccctggtcc aggtcgacac
    12601 gggcctggcc cacctcgacc ggccgttcga gtacaccgtg ccgacctcgt cggccgacac
    12661 cgcgctcccg ggtgtgcggg tcaaggtgcg cttcgccggg caggacctct ccggcttcgt
    12721 gctcgaccgg cgcgcggatg ccgagcacct cgggcggctc agcccgatcc gccgggtcgt
    12781 gtcgcccgaa cccgtgctca cgccagcggt gctgcgcgtg gcccgcgtcg tcgccgaccg
    12841 ctacgccggc tcgctcggtg acgtgctgcg cctcgccgtc ccaccccggc acgccgccgc
    12901 cgagaaggcc ttgcctgcct cgtcgccgga cccggctgac cgaccgccac tgccagatcg
    12961 gatgccggac gcctggctgc cgtaccccgc gggctccgcg ttcctcaccc acgtgggcgc
    13021 gggcggtgcg cccacggcat ccctgctcgc cgtcccgtcc accgacccgg acgccgactg
    13081 gccgatgctg ctggcccagg ccgcgcggac ggccgtcgag ggcggccgcg gcgcggtcat
    13141 cgtcgtgccc gaccatcgtg acgtcgtgcg ggtcgaggcc gcgttgaccc gctcgctggg
    13201 ccccggtcgg cacacccggc tcacggccga ccaggggccg caggccaggt acaccgcgta
    13261 tctcaaggca ctacgaggtc acgctcaggt ggtcgtcgga actcgggcgg cggccttcgc
    13321 cccggtacgg gacctcggcc tcgtcgcgtg gtgggacgac ggcgacgacc tgctcgacga
    13381 gccccgagcg ccctacccgc acgtgcggga ggtgctcctt gcgcgggcgg ccgacgaggg
    13441 cgctgccgtc ctggccgccg cgtactcgcg gtccgtggct gtggccgacc tcgtctcccg
    13501 gggcgtgctg accccggtcg aggcgccccg ggcggtggtg cgggcggccg tcgcgtcggt
    13561 gcgcgtggcc ggtgaggggc acgacgccga ccgggacccg gcggtcgccg ccgcccacct
    13621 gccgagcctg gcgtggcgca cggcgaagga ggcgctggcg cacggcccgg tgctggtgca
    13681 ggtcccgcgg cggggctacc tgccgtccct gtcgtgccag acctgccgtc gaccggcccg
    13741 gtgctcggcc tgcgccgggc cgctggcgct ggcaggaccg caggggccga cgtcgtgccg
    13801 ctggtgcggc cgggtcgaga cggcgttcac ctgcccggcc tgcggcgggc acgcgctgcg
    13861 ctccgctgtc gtcggcgccc gtcgcacggc ggaggagctg gggcgggcgt tccccggtgt
    13921 gcccgtcgaa cgctccgggg ccggcacggt cctcgacgtc gtcacgtcgg gtccccggct
    13981 cgtggtctcg accccgggtg ccgagcccgt ggcacctgag ggctacgccg ctgccctgct
    14041 cctcgacgcc tgggcactgc tcgaccgacc gacgctgctc gccggggagg aggccctgcg
    14101 ccggtggctc gccgcagcag cactcgtgcg gccggcatcg ggtgggggtc ggatcgtgct
    14161 cgcgggggca ccgaccgatg tgtccgtgcc cgccgtcgag gcgctggtgc ggtgggaccc
    14221 gacctggttc gccgagcgcg agctcaccga gcggcgcgag ctgtcgctgc cacccgcggc
    14281 ccgcctggcc accctcaccg gcccgcgcgc cgcgctggag cgggcggtcg cggaagtggg
    14341 gctcccggca tccgccgcgg tgctcgggcc gttgccgcac gcgggcgccg tcggcggcgc
    14401 cgggcccgcg ggcccggcac ctcaggagcg ctggcgaacg atcatcaccg tcccccggcc
    14461 cgacggcccc gacctcaccc gcgaactcgc ggcgatgcga gcccgggcat cagcccgcaa
    14521 ggaccccgac accatcaccg tgcgcgtcga cccacccgac cccacgacgt gaccgggctc
    14581 agcacgccgc agcccgagat gatgacggcc tagactcccc ggcgtgagtg tcacccccat
    14641 ccggctgttc ggcgaccccg tcctgcgcac cccggccgag ccggtcgtcg acttcgacgc
    14701 cgagctgcgc cgactcgtcg gtgacctgac cgacacgatg ctcgacgccg ggggagcggg
    14761 gctcgcggct cctcagctgg gggtcggcct gagggtgttc acctaccacg tcgacggcgt
    14821 catcgggcac ctcgtcaacc ccgacctcag cctcggtgag gacgaggagc gcgacctcga
    14881 gggctgcctg tcgctgcccg gtctgcactt cgagaccccc cgtgcggtgc aggtcgtcgc
    14941 ccgcgggtgg acgatgcacg gcgagccggc gaccgtcgag ggcacccacc tgctcgcacg
    15001 ggccgtgcag cacgagaccg accacctcga cggcatcctc ttcatcgacc ggctcgaccc
    15061 ggagcagcgc cgcgaggcca tgcgcctgat ccgcgaggcc gactgggggg cgaccccgcc
    15121 cgtggtccgc gccagcccgc acgccggcgc cttcgggagg cgttgagatg cggctcgtgt
    15181 tcgcgggaac cccggtgacc gcggtgccct cgctgctggc cctgctggcc tccccgcacg
    15241 acgtggtcgc cgtgctgacc cgccccgacg cccgctccgg cagggggcgc acgctcgcac
    15301 cgtcgccggt caaggaggtg gcgctcgagc acggtctcga ggtgctcacc ccggtctcac
    15361 ctcgcgaccc cgacttccac gcgcggctgg tcgagctcga ccccgactgc tgcccggtcg
    15421 tcgcctacgg tgccctcgtg ccccgcgccg cgctcgacgt gccgcgggtc ggctgggtca
    15481 acctgcactt ctcgctgctt ccggcctggc gtggggccgc ccccgtgcag cgcgcgatca
    15541 tggccggtga cgacgtcacg ggcgccacga cattcctgct cgaggaaggg ctcgacaccg
    15601 ggccggtgct cggcaccctc accgagacgg tgcgcccgag cgacaccagc ggcatcctgc
    15661 tcgaccgcct cgcccacgcc ggcgcgggcc tgctcgtcgc gacgatggac ggcatcgccg
    15721 acgggtcgct ccacgccgtg ccccagcccg atgacggcgt cagccacgcc cccaagctga
    15781 cgaccgacga ggcgcaggtg cggtgggggt tgcccgccca cgtcgtcgac cggcacgtgc
    15841 gcgggtgcac gcccgccccg ggggcctgga cgacctttcg cggtgagcgc gtcaagatcg
    15901 gctccgtcgg gctcaccgcg gcagcagcgc ggatgccgtc cgcggacttc gtggacgccc
    15961 cctccctcgc gctcgccccc ggtgagctgc acgtcaccaa gcgctcggtg ctcgtcggca
    16021 ccgggtccac ccccgtcgag ctgcgggagg tgcgtcccca cggcaagaag gtgatggccg
    16081 cgcccgactg ggcccgcggc gtccggatca ccccaggaga acgtttcgat ggctgagcgc
    16141 gagcaccacg accgcagcgg ttcccgaccg cagcggcgcg acgaccgcgc gggcggccga
    16201 ccggcatccg gcggcgggcg tggaccacgg gcgcgcagcg ccaccgcacc ctcccagcgc
    16261 accaggcggg ccgagccgac ccggttcgcc gcgtacaccc tgctgcgctc cgtcgccgag
    16321 ggtggttacg ccaacctcga gctgcccgac atcctgcggc gccaccacct cgccggccgc
    16381 gatgccgcct tcgccaccga actggccttc ggcacgatcc gctggcaggg cttctacgac
    16441 gccgtcatcg ccgaggccgc cggccggccc accgagcgga tcgaccccca ggtgctcgac
    16501 gtgctgcgcc tcggggccca ccagctcatg gggatgcgcg tgtcgaccca cgccgcggcc
    16561 gaccagaccg tgggcctgtc caaggtcgtg gcgggggccg gggcgggcag cttcgtgaac
    16621 gccgtcatgc gacggatgag cgagaggact ctcgaacagt ggcaggaggc agtcgtcccc
    16681 gagggcgggg gagtggacgc cctcgcggtg cgccacagcc acccccagtg ggtggtctcc
    16741 gcgctgcggg ccgccctgct cgggcacggg cgagccaccg ccgagacgat cgacaccgag
    16801 ctcgaggcgc tcctggccgc cgacaacgac ccgccccgcg tccacctcgt cgccaggccc
    16861 ggtctgtcgg acgtggacga gctcctgtcc tccgcggagg cccgcaagag caccctgtcg
    16921 ccccacggcg tggtgctcga gagcggtgac cccggggccg tcgcggccgt gcgccagggc
    16981 cgggcgtcgg tgcaggacga gggctcccag ctggtggccc tggcactggc ggcggccgag
    17041 gtgggacccg acgtcccgga ccgctggctc gacctgtgcg cggggcccgg tggcaaggcc
    17101 ggggtgctcg gcgcgatggc ggtgcagcac ggcgccgacc tcaccgccgt cgaggtgacc
    17161 ccccaccgtg ccgacctcgt gcggaccacc ctcgcggccc tgacccggca cgccgagtcg
    17221 gtcggccagc gcgtcgaggt gcgcacggcc gacgggcgtg acgtgggcgg ggaggagccg
    17281 ggcggctacg gcaaggtgct cgtcgacgcc ccgtgcaccg ggctgggcgc gttgcgccgc
    17341 cgccccgagg cgcggtggcg ccgccagccc tccgacgtcg ccgagctggc gccgctgcaa
    17401 cgggcgctgc tgacctcggc gctggccgcc gtcgccccgg gtggggtggt cgcctacgcc
    17461 acgtgcagcc cgcacgtcgc cgagaccagg ttcgtcgtct ccgacgtgct gaagaagcac
    17521 cctgacgtcg aggtcgtcga cgcccgaccg ctgttcaccg acgcctccgg ggcgcccgtc
    17581 gacgggctgg gtgacgggcc gtacgtgcag ctgtggccgc acgtccacgg caccgacgcg
    17641 atgttcttcg ccctgctgcg tcgccgctga acaccgtcac ggcctagggt tgcgcccgtg
    17701 ctgatctcac cgtcgatcct ctccgccgac ttcgcgaacc tcgagcgcga gctgggggcg
    17761 atcgccaacg ccgactgggc ccacgtcgac gtcatggaca accacttcgt cccgaacctc
    17821 acgctcgggc tgcccatcgt cgaggcgctg gtgcgggtgt cgcccatccc gatcgactgc
    17881 cacctcatga tcgaggaccc cgaccgctgg gccccggggt acgccgaggc cggggcgcgc
    17941 tcggtgacct tccacatcga ggccgtcgcg gatgccgtgg cgaccgctcg cgcgatccgc
    18001 gccgctggtg cccgggcggc gttcgcggtc aagccgggaa cgcccttcgc gccctacgag
    18061 gacctcctgc ccgaggtcga catggtgctc gtcatgaccg tcgaacccgg gttcggaggc
    18121 cagtcgttca tggccgacca gatgtccaag gtcgcgcagg tccgcgaggc cgtgcgccgc
    18181 cacggcggcg aggtgtgggt gcaggtcgac ggtggggtgt ccgcctcgac gatcgagcag
    18241 tgtgcggatg ccggtgccga cgtcttcgtc gcaggctccg cggtgtacgg cgccgagtcg
    18301 gcgtccgcgg cgatcgagca gctgcgcgcg ctcgccaccg cccacaccca ccggtgagcc
    18361 acccggcatc cctgctcgac gaggtcgccg ccgaccgggg ctacgtcgtg ggccggactg
    18421 ccggttcgtc gactgcacgg cgctgatctg cgcggcgcga gcctggtggg cgcctacgga
    18481 tacaccgagg cgaaggctcg gaagatctgt gcaacgttgg acaaagattc aacacagttc
    18541 gcctagagtc gtgtgcatgg atgtccatgc cgccctgacc gacgttcgct cctcagccac
    18601 catcctcgac gggatgcgcg ctggcgaacg gctcgaggac gtcgcccgtt cggccggtcc
    18661 tgaggtggtc gaccacctcg tcgtcgccac cacggacccc gatccgatca ccgcgatcgc
    18721 agcggtgcac gccctcggcg cccacggcag cgcaacggcc ggggcgcacc tggtcagcct
    18781 cctggacgac gagcgagccc acgtggcgga gcacgccatc gacgccctgt ccggggtggt
    18841 tgcgcaggcc gcggccctcg agcggctcgt ccggcactgc gccgagggcg gctttccggg
    18901 gatgctggcg gcgcgcaccc tggagcggtg ggccgcggcg tctccggatg ccgtcgagca
    18961 cgccgtgacg gtcggcctcg cgtcggagcg cgacccggct gcccgggcgc acctggagga
    19021 gctggctcag gacctgcgca gcggtgcccg cgacgccacg aagccgccga cccgcggggg
    19081 gccgggcctc accgtggccc agctcttcct gcacggtgac ctcgacggca gcctgcgcca
    19141 ctccggccag ggcgacaccg gcggggtggc aacgctcctc gtgcacctcg gggacgccct
    19201 gctgcgtcac ggcgcccggg tggaccgggt gatcaccatc tcggggagca gggatgccgc
    19261 ggtcgccgac gcgtgggcag ccggcgcgac caccggccgc accctctcgc aggacctcgc
    19321 cccgagaggc caccactacg cgcgcatccc gttgagcggg ccctcgacga gcgtgtcgac
    19381 ggcgtggtcg ctgcgcatcc aggcgcgcca cggcatccga gaggtcctgc gcgccgcggg
    19441 tcgcgtcgac gtcatccacc tgcggatggc ggacgtcggg tcgctggtcg cggccgaggt
    19501 ggcggccgag ctcggcatcc ccgtggtgct gaccctggcc ccggatcccc aggcgctcct
    19561 cgccgcccga gaggccgacg ggagcctgac ccgcgccgag ttcggcgacg cagaccgggt
    19621 ggagcacctg tggtttcgcg accggctgct gcgggccctc gccgcgcagg ccgagcacct
    19681 cgtgctgttc ccacgcccgg acgtcgagcg cagcatcggg cggctggtgg gcatcgacgt
    19741 gacggcacag cgcgagcggg tgtccgtggt cggcgagggc atcgacctcg cggccctcga
    19801 acgcgtcgcc aaggaggtcg ccggcgacgc gccgcggagc ccggagacgg catccgcgct
    19861 ccgggagctg agcgccgtgc tcgaccagct tcccccgaac cggcgtggcc tgcccctcgc
    19921 cgtgtcggtc ggccggctgt ctccggtcaa gggcatggcg acgctggcgt cgacctggct
    19981 caccgagccc accctgcggg aacgctgcaa cctcgtcatc gtcggtggtg acctggacga
    20041 cccgacgacc gaggaggccg ccgaactcgc ccgcatccga gaggtcgtct caaccgacga
    20101 ctgcgccgga gaggggctgc tcctcgccgg ccaccgcccg aacgccactg ccaccgcgtg
    20161 gctcgcagcc gcccaccggg gcgtccccgg ggtgtgtgcg cccgacgggg tctacgtctg
    20221 cgccagcctc aaggaggagt tcggcatcgc gatcctcgag gcgatgacga tgggcctggt
    20281 cgtcgtggcc ccgaacagcg gggggccggc gacctacatc gaggacggcg tcacgggtgt
    20341 cctcaccgac acgaccgacc cacgacggct cgcggacgcg ttcggccgtg ccctcgagct
    20401 cgcctcgtca ccgggtgagg cgctgcggac ccgccgctca cgggcagggg tccgcgagcg
    20461 gttcggcatc gacacgatgg cctcgtccct ggtcgaggtg tatgccgcgg tggcggcgcg
    20521 ccaggccgcg acgtcgctgc cgagggcggt cacgtcatga ccctgctcgt catcagcccc
    20581 gactacgcct cccacctgta cccgctcgcc accctcgcga cggcctggca ggccgagggt
    20641 gagcgagtgg tggtcgccac cggcgcggcc accgacgccg tggtgcgggc gttcgggtac
    20701 gaccgcgaac accttcagct cggccggggg tcgaaccccg gggtcatccg ggccgaggag
    20761 cagccgccgg gggaggacga cgcactgcgc ggcttcttcg acgccacccg ccgcggcgcc
    20821 gtggccgccc tcgagttcca ggcacgggcg cgcggcgatg acctgctgtg gaacgcggtc
    20881 ggcgtggcgc gcgaggtcca ggccatcgtc gagcgcgtgc agccggatgc cgtgatcgtc
    20941 gaccacctgg ccttcggggc ccgtttgggg ctggtgggcg cgcaggtacc gcacgcggac
    21001 gtggtgctcg gccacccgac tgcactcacc gtcggtgacg aggtctacgg ccacccaccg
    21061 acctggccgc gtgccatgcg ccccgctgac gaggagctcg ccgacctgcg ggtgctgtgc
    21121 gaaggggtcc gtgacacgtt cacggcccag tggaacgcag cgctgaagga cctgcgcccg
    21181 agcgcgcagc ccagccggga cgccttcgcc gagaccggcg acctgctgct gctcaactac
    21241 ccggccgcgc tccacgagcc cgagcgcacg gccctgctcc cgccgcacac cttcctcggg
    21301 tcggcagtgc gcgaggaacc acgggacgca caggtcgagg cctggctgga cgccgacgag
    21361 gggagcgtcg tgtacgtgag cctcggcagc ttcctgtcgg cccgctccga cgtgctcgcg
    21421 ctgatcgccg acgccttgcg acggctcgac gtccgggtgg ccctcgcgac cggctccgcg
    21481 ccggtggagg agctcgggcc gctgccgcag ggttggctcg tgcgcgagtt cctcccgcag
    21541 gtgcgcctgc ttgccgcgtc cagcgtcgcc gtgacgcacg gcggcaacaa cagcgtcacc
    21601 gaggccctga ccgccggggt tcccctgctc gtcctgccgt tctcgaccga ccagttcgcc
    21661 ggtgccgcgg cgatcgagcg caccggggtc ggggtctgcc tggaccccaa cgccaccacc
    21721 tcgggcgaca tcgcggatgc cgtgcggcac cttctcgccc cgggcggcgc ccgcagtgcc
    21781 gcggcggcga tcggcgaggg gctgcgcgag gttcccggtc cgcagcgagc acgggccgcc
    21841 gtgcgccagg cgctcagcgg ctgacgtcgg ctgaccggtg acctcgtgag atcacctgga
    21901 gcaggtcggc ggcgacggcg acggcgatgc tcgcaggctc cttgccggcc aggtcgggca
    21961 cgccgatggg ggagtggatc cggttcacca cgtgcgccgg gtggccgtcc gccgcgaagc
    22021 gttgctggaa ccgggtccac ttggccgagg agccgatgag tcccacggat cccagtcccg
    22081 ctcgtcgcag ggctgcgtcg cacagggcgt agtcctcggc atggtcgtgc gtcatcacga
    22141 cgacgtgcgt tccagcgggc agcgagtcga gcaccatctc cggcaggggt gagtggtgcc
    22201 gttcgacgcg ggccacggcc tcgccgagcc acgacatcat ggcgacgtcc agctgctcct
    22261 cgcgggagtc cacgaggtgc aggtcgaggt cgtgacggct gaggatgcgc gcgagctcgt
    22321 ggccgacgtg cccgacaccg aagacggcca cgcacggcac gacggccagg ggttcgagca
    22381 ggacgctgac ctcgccgccg cagcactggc ggccgtgctc ggtgcgcgcg cgctcgctga
    22441 gggtgatgtc gagctgctcg gggacgtctg cgccgtccgc cagcatccgc cgagcgcgct
    22501 cgacggcggt ggcctcgagg ttgccgccgc cgatgctgcc ccaggtgtcc cggtcggaga
    22561 cgacgagctt cgcgccggcg tcgcgcggcg cgtgtccgcg aacccgggtc accgtcacga
    22621 ggacaccggg ctggcgggag gtgcgcaggt ggtcgagcgc tgcgagccag tgcatgtcag
    22681 ccctccacga ccgggtgcag acctgggggc acgcccccgc cgcgagcgtg ctcgactgcc
    22741 cagaagacgg cttccggcgt ggctggcgtg gccaggccca ccgagacccc ggggggcccg
    22801 aacgctgctg cggcctgacg aagggcctct cgcaccgaga acgccagcat gagcgggggc
    22861 tcgccgacgg ccttcgatcc gtagacggcg ccgtcctcgt gggcgcgctc gagcagggtg
    22921 acgttgaact cctcgggcat ctcggagaag ctcgggagct tgtaggtgct ggccgtgttc
    22981 gtcgccagcc ggccccgcga gggcccgtcc gactcgtccc accgaaggtc ctccagggtc
    23041 aaccagccag ccccctggac gaacgccccc tcgacctggc cgaggtcgat gaggggggac
    23101 agcgagtcgc cgacgtcatg gacgacatcg acgcgcagca cccgatggct gccggtgaag
    23161 ccgtcgacct cgacctcggc cgcggcgacg ccgtaggaga agtacttgaa cggggagccc
    23221 tgcatggccg cggcgtccca gtgcagcccc tcggtgcggt agaagcccgc cgcccacagc
    23281 tggacgcggt cggcgtaggc cactgccacg agatcggccc agggcagggg ctctgcatgg
    23341 gcagcctcca ccgcgcgcag ccgctcgcgg atctggacgc aggcatcgag gacggcaccc
    23401 ccgttgaggt ccgccccgga gctcgcggcg gtcgccgacg tgttcggcac cttgtcggtg
    23461 cgcgtcggcg cgaggcgcac caggtcgagg ggcacgtcga gcgccgtggc cgcgatctgg
    23521 agcatcttgg tgtgcaggcc ctggcccatc tcggttccgc cgtggttgac gagcacggac
    23581 ccgtccttgt agacgtgcac gagagccccg gcctggttga aggcggtgaa gttgaaggag
    23641 atgccgaact tcaccggcgt caccgcgatc gctcgcttgg agtgctcgtg gctcgcgttg
    23701 tactgcgcga cctgttccag tcgccgggcc acgtccccgg aggacagcac ctgctcccag
    23761 caggtgacga tccgctcggc gtgtcgcacg acctggccgt agggggtgct ctggccctcg
    23821 cggtagacgt tgcggcgccg cagggagagc gggtcgatgc cgagcagcgg ggcgcaccgg
    23881 ccgaggatgt cctcgatgac gagcatcccc tgcgggccgc cgaagccgcg aaaggcagtc
    23941 tgcgacgtct tgtgggtccg ggcgatccga ccatcgaccc gcacgttcgg gatccagtag
    24001 gcgttgtcga cgtggcagag ggcacgagcc atgacgggtt cggacaggtc caggctccag
    24061 ccgccgtccg aggtcagggt ggcctcgagg gcgaggaggt ggccgtcgtc gtcgaacgcc
    24121 acctgccacc gggtgtggaa gccgtgccgc ttgccggtca tggtcatgtc ctgcccgcga
    24181 gtgagtcgaa cgcgcaccgg acgcccggtg atcaccgcgc ccagggcggc gacggccgcg
    24241 aacccgtggg gctgcatctc cttgcctccg aagccacccc ccatgcgcag gcactgcacg
    24301 gtcacggcgt gggtggctcg accgagcacg tgcgcgacga tgtcctgggt ctcgctgggg
    24361 tgctgggtgc tgctctggac gaagacctgc ccgctctcgt cgacgtgggc gatcgagcag
    24421 tgggtctcga ggtagaagtg ctcctggccg gcgctggtcg tcacgccgct gaagacgtgg
    24481 gcggcctgtg cgaggccgct ctcgacgtca ccgcgttcga ggcggggcgc accgccctgg
    24541 aagctgccgg catcgatggc ctcctcgacc gtcaccacgg ccggaagcac ctcgtactcc
    24601 acctcgaccg cgagggcccc gaggcgggca gcctccgcgg tctcggcgag cacccagcag
    24661 accgactggc cgtggaacat cacctcgtcg gggaacagcg gctcgtcgcc cttggccccg
    24721 gcgtcgttga tgccgggtac gtccgcggcc gtgagcaccc gcacgacccc gggcaccgac
    24781 agcgcaggct cggcgtccag acgggtcacg agggcgtgcg cgtgcggagc ctggacggga
    24841 tgcgcgtgca gcagccccgg catccgcgac ccgaggtcct cggtgtacag cgcccggccg
    24901 gtgacgtgca gggccgccga ctcgtggggc agcggctggc cgacgacctg gtcgtcaggg
    24961 ccttgggcca cggcgctcat cgggtcacct ccggcgtgct cgcgccctgc gcgtgcagcc
    25021 gcaccagggc ggtggcgagc accgcccggc ggtaggcggc gctggcgcgg tggtcgtcga
    25081 tcggggtgcc gacgtcgcgc agcacctcgc cggccgccgc ggcagtcgcc cgtgtccacg
    25141 cccgcccttc gagggcggcc tcggtgtcgg tcgcccgcag gggagtggcg gcgacaccgc
    25201 ccaccccgat ccgggcgcgc tgaacgctcc cgtcgacgac gtgcagcgac accgcgaccg
    25261 cgacgctggt gatgtcgtcg tggcgtcgct tggcgatctt ctggaacccc gtgacctcac
    25321 cgagcggcag ggggatgcgg acggcgcgga tcagctcgtc gcggcgccgc acgctgcgcc
    25381 ggtaccccgt gaagaacgat gcgaggggta ccagtcgctg cccgtcggtg ccgtggagca
    25441 cgacgtcggc ctcgagggcc agcagcgccg gggcgaggtc gccgatgggg gagccggtgg
    25501 cgaggttccc gccgatggtg gcgctgttgc ggatgagcgg ggacgcgaac agcggcagca
    25561 cggcggtgag cagcggcacc tcccgcgcga tgccccgctc aagctcggcc agggtgaccg
    25621 cggccccgat ctcgaggtgg tcggcgccac gggtgaccgc ccgcagctcc gccaggtggt
    25681 cgacaccgac gaccagctca gcccgggcac cccgaaggtt gacctcgacc ccccagtccg
    25741 tggaccccgc cacgacaacg gcatccggat gccgctggag caggtggatc gcctcgtcga
    25801 ggtcggcggg tcgcacgtag gagccggtgg gaccgtccac gaccacgggt gcagcggcgg
    25861 gtccggggcc ctccagccgg ctggcgagcg cgtcgtcggt ctgcgggagg ccgagcgcga
    25921 aggcggcgtc gcggatcgga cggtagcccg tgcaccggca caggttgccg gagatggccg
    25981 tgaggtcgaa gccgttcggc ccgacctcgc ccccgtggtc cttcggtgac ggcgcccggt
    26041 ccggacggta gaactcggcg gccatcgagc acacgaagcc gggcgtgcag tagccgcact
    26101 gcgacccacc gcgcacggcc atctcgtgct ggacggggtg cagcgcctcg ggcgacccga
    26161 gcccttcgga ggtcacgacc tcctgacccg ccagggcgct ggccgggagc aggcaggcgt
    26221 tgaccgcggc ccaccgggtg cggtcatcgc cgtcgcgccg ggccagcatc accgagcagg
    26281 caccgcactc accctcggcg cacccctcct tggctccggt cagcccgatc gagcgcagcc
    26341 agtccagggc gggggtccac gggtcggtgc cggcgaggtc gtggatcaca ccgttgaccg
    26401 tcacccgggg ggtctggttg gtcgccatgg cctgatcctc ctcgtcgtcg cggcccgtgg
    26461 ggcccttgtg cccagcggca tccgggtgag ggagcacaca cggcgaggcc acggtaaccc
    26521 cgcgccgggg tgcggcatac tgggcgtgac acgtgctccg gggtcggtgt aattccgaac
    26581 cggcggtgac agtccgcgac ccgaccgcag ccagcggccg gttgacccgg tgaaactccg
    26641 ggaccgacgg tgaaagtccg gatggtaggc agcacgtgct ggcgaggccc gcgggtctcg
    26701 ccggcggtcg tcgtggtcgg ttgccctcgg gtgccaacct tggacaacca tcctcccgga
    26761 gcctgaccga acacccggtt tccaggcacg agggcaggac atggacgaga gcaccgcgct
    26821 gatgcagcgg gcgatcgacg ctgctcgcct cggcccgcag gccgacccca acccgcgggt
    26881 gggctgcgtc gtcacggatg ccgcgggtcg ggtggtcggt gtcgggcacc accgcggggc
    26941 cggcaccgcg cacgccgagg tcgacgcgct cgcccaggcg ggggaggcgg cccgcggcgg
    27001 caccgcccac gtcaccctcg aaccgtgcaa ccacaccgga cgcaccggcc cgtgcaccgg
    27061 cgccctgctc gaggcgggca tcgcccgggt cgtcttcgcc gtcggggacc ccgacgcggt
    27121 cgccgcgggt ggggccgcga cgctggccgc cgccggggtc gccgtcgaga ccggggtcct
    27181 gcgcgaggcc gccgaggaac tcaacgagtc gtggctgcac gcccgccgca ccggccgccc
    27241 gttcgtcatc gccaagagcg cctcgaccct cgacggtcgc acggccgccg ccgatggcac
    27301 gagtcgctgg atcaccggcg aggcagcccg cgccgacgtg cacgacctgc gctcgcggtg
    27361 cggcgccgtc gtcgtcggca ccggcaccgc cctgagcgac gacccccagc tcaccgtgcg
    27421 cacagccgac ggcacggccg ccgggtccca accgctgcgc gtcgtggtcg gccaccgcac
    27481 cctcccggcg acggcgcgcg tgctcgacgg cgcggccgag accctgctgc tggcgaccca
    27541 cgaccccggc gcggtgctcg cggcactgca cgcgcgcggc atccaccgcg tcctcctcga
    27601 aggtggcccg acgctgaccg cggccttcct cgccgccggc ctcgtcgacg aggtcgtggc
    27661 ctacgtcgcg ccggcgctgc tcggctccgg ccccacggtc agtgccgacc tcggcgtgcg
    27721 caccatcgcg gatgccgtgc gcctggacct cgtcgacgtc accgtcctgc cccccgacgt
    27781 gcggatcacc gcccgccccg ccaccgcacc cccggtccac ccccgggcaa ggagctgatc
    27841 gccgtgttca ccggaatcgt cgaggagctc ggcaccgtgc tcgccctcga caccctcgag
    27901 gcagacggcc gcaccgactc cgcgcgcctc accgtcgcgg gcccgctcgt gaccgccgac
    27961 gccacccacg gcgcgtcgat cagcgtcaac ggggtgtgcc tcacagtcct cacccacggg
    28021 gacggggagt tcacggtcga cgtcatggcc cagacgctcg cgctgaccag cctgggcgcg
    28081 ctgcgaccgg gcgaccgggt gaacctcgag cgcgcgatgg cagccgacgg tcggttcggc
    28141 ggccacgtcg tgcagggcca cgtcgacggg gtcggcacca tcgtgcgccg gacgcccggc
    28201 gagcggtggg aggtcgtcga ggtctcgttg cccgccgacc tcgccggcta cgtcgtcgcg
    28261 caggggtcga tcaccgtcga cggggtgagc ctgaccgtgg ccgggctcac cgggacgacg
    28321 ttcagcgtct ccctcatccc gaccacgctc gcgctgacca cgctcggcac caaggacgtc
    28381 ggtgaccccg tcaacctcga ggtcgacgtg ctcgccaagt acgtcgagcg cctgctcgcc
    28441 acccggttcg ccagccccgc agcccagcca acaccgaccc ccgcggagga gtcatgaccc
    28501 tgtcgaccat cgaggctgcg ctcgacgcgc tgcgcctcgg gcgcccggtg ctcgtcctgg
    28561 acgacgagga ccgtgagaac gagggcgacg tcgtgctcgc cggtcagacg ttgaccgacc
    28621 agtggatggc ctggaccatc cgcaacacct ccggctacat ctgcgccccc gtgaccgagg
    28681 aggtcgccga ccggctcgac ctgcccctga tggtccgtga caaccgcgac ccccgacgca
    28741 ccgcgtacac ggtgaccgtc gacgcggcat ccggtgtctc caccggcatc tcggccgccg
    28801 accgggccca caccatccgc accctgtccg acccgacggc gagcgccgac gacctcatcc
    28861 gaccggggca cgtcgtgccg ttgcgggccc gtgccggggg agtgctggcc cgcgccgggc
    28921 acaccgaggc agccgtcgac ctgtgccggc tcgccgggct cgaaccggtc ggagccatcg
    28981 gtgagctcgt cgccgacgac ggcaccatgc tgcgcacccc gggcgtgctc tccctcggtg
    29041 ccgcccacga cctgccggtg gtcaccatcg ccgacctcgt ggcgtggcgc cagcgccacg
    29101 accgggtgac gcgggtcgcc gacaccgtgc tgccgacgcg ccacggcacg ttccgcaccg
    29161 tcgcctaccg cgacgtgctc accggcgtcg agcacctcgc cctggtcagc cccctgggca
    29221 tcccggagcg ggccccgctc gtgcgggtgc actccgagtg cctgacaggt gacgtgatgg
    29281 gttcgcagcg ctgcgactgc ggcccccagc tcgagcgttc cctcgagcgc gtcgcggccg
    29341 agggtggcgt cgtcgtctac gtgcgcggac acgagggccg cggggtgggt ctcggtgcga
    29401 agctgcgcgc ctacgagctc caggaccgcg gtctggacac cgtcgacgcg cagatcgagc
    29461 tcggcctgcc gatcgacgca cgggagtacg ccgctggcgc tgccgtgctc ctcgacctcg
    29521 gggtgcacga gctgcggctg ctcaccaaca accccgccaa ggtcgatgcc atgcgcgagc
    29581 acggcatccg ggtcgtcgcc gtcgagcgac tgcggatcgc acccgtggcc gagaacgccg
    29641 cctacctgcg caccaagcgc gaccggatgg ggcacgacct cgtcatcgac agcagtgaag
    29701 gagcagcacc ggcatgagtg gacacggagc acccgtcgtg accgtcgacg gacgcgggtt
    29761 gcgcgtcgcc gtcgtggccg cgtcgtggca cgagcaggtc atggacggcc tcgtcgacgg
    29821 cgcgttgcgc ggcctggcgg atgccggggt gcagtcacct ctcgtggtgc gcgtgcccgg
    29881 cacggtcgag ctgagtgtcg cctgtgcccg gctcgcgccc cgattcgacg ccctcgtcgc
    29941 cctcggcgtc gtcatccgtg gcggcacccc gcacttcgag tacgtgtgcc agggggtcac
    30001 cgtcggcctg acccaggtgt cggtgaccac cggggtgccc gtcgggttcg gcgtgctgac
    30061 ctgcgacacg gacgagcagg ccgtggcacg ggccggcctg cccggcagcg tcgaggacaa
    30121 gggccacgag gccgcgaccg cagccgtcgc cacggccgtg accctgcgcg acctggatgc
    30181 cgcggcggcg ggtcggtgac cgtgctgcgc gagctgctcg cgtggctcca gcacgagtcg
    30241 atcacggtgg cggggtaccc gctgccgtgg atcgaggtcg tcggcaacct cttcgggttc
    30301 gcctcggcca tcctcggcat gcggcgcaag gtctgggcct ggcccatcgg gatcgtcggg
    30361 aacctgctgc tgttcaccat cttcgtctcg gcgaccttcg acgccgactc gcgcaccccg
    30421 ctgttcggtc aggccggccg gcaggtcttc ttcatcatga ccagcctcta cggctggtgg
    30481 cgctggcagg agcaccgcaa cgcccgcctg gcggccggcg cgtccgccga caccccggcc
    30541 atcaccccgc ggtgggcgac cgcgcgcgag cggctcggct acgtgctcgt gtggatcggt
    30601 ggggtcctgg tcgctcagtg ggccctggcc ggcatcggcg cggcgtggcc cgctccgcgc
    30661 tggtacttct ggtgcgacgc gtggatcctc gtgggctcga tgctcgcgac gtacgcgatg
    30721 gcccgcgggt ggaacgagtt ctggctgatg tggatcgccg tcgacctcgt cggcgtgccg
    30781 ctgctctggc acagcgagta ctacgccacc gccgtgctct acggcgtgta cgggttgctc
    30841 gtgctctacg gcttcttcgt gtggttgcgg gcttcgcggg ccgaggcacc ggacgcgctg
    30901 gagacccgcg agctcgaccc cgtcgcctga ccggacggac ggcgccgtcg agggtcgccg
    30961 cgcccacgta ggcttgtccc tcgtgaagac gttcgacgag ctgtttgccg agctggccga
    31021 caaggcccgc acccgcccgc ccggttcggg caccgtcacc gagctcgact ccggggtcca
    31081 tggcatcggc aagaagatcg tcgaggaggc cgccgaggtg tggatggcgg ccgagtacga
    31141 gggccgcgag cgcaccgccg aggagatcag ccagctgctc taccacctcc aggtgctcat
    31201 gctcgcgtgt gacgtcaccc cggacgacgt gtacgcccat ctctgagggc cgctccggca
    31261 tccacccctg aacgccacca cggcatccac ccctgacgaa agcctcacca cccatgctgc
    31321 gcatcgccgt ccccaacaag ggttcgctgt ccgagccgtc ggccgccatg ctccgcgagg
    31381 cggggtaccg cacccgtcgc gaccccaagg agctcatcgt caccgacgcc gcgaacggcg
    31441 tcgagctgtt ctacctgcgc ccgcgtgaca tcgccgtcta cgtcggctcc ggcaccgtcg
    31501 actgcggcat caccggccgt gacctgctgc tggactccgg gtcggccgcc gtcgaggtgc
    31561 tcgcactggg cttcggcgcg tccaccttcc ggtatgccgc ccgcccgggc acggcgtccg
    31621 cgctcgccga catcggtggc caccgggtgg cgaccagcta ccaggggctc gtcgagaagc
    31681 acctggccga ccacggcatc caggctgagg tcgtgcgcct cgacggtgcg gtcgagacgg
    31741 ccatcaccct gggcgtcgcc gacgtcatcg ccgacgtcgt cgagaccggg gccaccctgc
    31801 gatcgcaggg cctcgaggtc ttcggcgacc cgatcctcaa gagcgaggcg gtgctcgtgc
    31861 gccgcgaggg cagcgaggag gcggcccagg tcgaggtgtt gcgccgccga ctgctcgggg
    31921 tgatcaccgc gcgccactac gtgatgctcg actacgacgt gcccgccgcc ctcgtggatg
    31981 ccgcctgtgc catcactccg gggctggagt cgccgacggt gtcgaacctg cagaaccgcg
    32041 actggtgcgc cgtgcgtgcc atggtgccgc ggtcggggac gaacgcggtc atggacgagc
    32101 tctacgacct cggcgcccgg gccatcctcg tcaccgacat caccgcctgc cggctgtgac
    32161 cgacaccgga ggttcgtccg gcccggtggt gttccgaccg cgccgcgggc gggtcgtgcc
    32221 cctggtcatg gcgacgatcg ccctcgtcgt cacggccggt gtggccctgg gcatgggagg
    32281 cgccgggtgg tcgggtggtg accagctggc gttgttcggt ctcggcgccg ggctggcggc
    32341 cttcctcggt cggtacgcga gcatccgggc cgtgggcgac gacgtcggcc tgaccgttcg
    32401 caacctgctg atcacccgca cggtgtcgtg ggaggagatc gtcgaggtgc gcttccccga
    32461 tggcgcgccc tgggtgagcc tggagctcac ggacacggac gagctcgccg tcatggcgat
    32521 ccagcgggct gacggcgacg acgccaaggt cgaggcccgc cgactcgccg agctcgtggc
    32581 cgagcgccgg accacccgct gatcccgctc agtcgatctg ggcgcccacc cgtgcgaggt
    32641 ccggccgcag gcgcagcgac aggatgagct gcggcaccat cacgagcgcg agcagcgccc
    32701 agaccagcga gaagctgccg gtgacgtcct cgaccacacc catcgccgcc gggccgaacg
    32761 ccgccgcggt gtagctgacg aggaaggcca tggccgtgag gcgggcgctg tcgcgcggtg
    32821 tcgccgcgta tcgcacgagc agcgccaacc cgagcgcgaa cgccgccccc tggccggctc
    32881 cgagcaggcc agcccacagc cacggggcgg tgtccggggc cagccacacc ccgagctccc
    32941 cggcgccgcc gagcaggccc gcgagcacga gcagcaggcg cgggtcgggg acgtggtcca
    33001 gcagggtcgg ggcagccaac ccggacagca gctgggcgcc ggtgaaggcg gccatgagca
    33061 ggccagcctc gcgcgcggtc cagccgtgcg cctcgtacgt gggggccagc cacgcgagcg
    33121 cggagtagaa ctgccaggac tgcgccgtga ggtaggccgc gacgagccaa gccgtcgcgc
    33181 tcgcccacgg cagcgcatgg gtgacgtcct cgggcacctc ctcccgctcc ggatgccggt
    33241 gcgccgcccg cgcgaccggc acccacacca cgagcgcgag cgcggcgagc ggcgtccaca
    33301 cgagcagcga acgtgaccag tcgcccagga cgtcggcgag cggtaccgac gtggccgagg
    33361 cgaccgcagc caggcccatc atcgcggcca tcgtgaggcc tgtgccgagc ccggaccgcc
    33421 gtgcggggaa gacggccttg acgatgcccg gcagcagcgt gccggcgagc gcgatgcccg
    33481 cgccggcgac gaacgtcccg gcgtacagcg accacaccgc gccgccaccc gatccgcgca
    33541 gcacgagccc ggcgagcacg agcccgaccc cggctccggt ggcgacctcg gagcccagcc
    33601 gtcgagcgac ctggttcgcg ccgggtgcga gcagccccat gcagagcacc ggcagcgtcg
    33661 tgagcacgcc catccacgcg ctcgacaggc ccaggtccgc ccgcaccgcc tcggcgagcg
    33721 gaggcaggct cgccatgagg gtgcgcatgt tggccgcgac cagcacgatc gcagccacga
    33781 gtgcccaccg cggtcgctcg gtcacgccga tgactcgggc agcgagagca aggcgtcgtc
    33841 ctcgtccatc cctgcgatga ccagcaggtc gaccaccatc gaccgcacct gtccgcgcag
    33901 catctccgcg gacagacccg cgtcagggtc gacgtgcgcg gagagctcgg cgacacggtc
    33961 gagcaccgcc cgcgcggcgg agggcgtcgc gtgctcgtcg aggacccggg ccaggtcgcc
    34021 cgcggcatcg gccagcgccg ccacgagggc gatgtactgc tcgggcaccg cttcgccccg
    34081 gacgagcgca acgaccgccc tgcgtgcgag cacgcgcagg ttgcgcacgc accggtcgag
    34141 cggcacgacg agcgccgatc ctgccctggc ggctggggcg tgcccgcgca gcagcggtga
    34201 ctggcgcacc acggcggcgc cttcggaggc agcgacccgc agctctgcca gctggtcctc
    34261 cagggcgcgc gcaccgtcaa gggcggcggt ggcgagcgcc cggtcacgac ggtgcagggc
    34321 gtcgaccacc tcgcgcaggg tggtcgcgat ctcccgcgcc gtcctcgccg cgagcacgcg
    34381 ggggcggcgc accggtgacg tgggcgcgat ggccccgacg agcagggcga ccgcgccgcc
    34441 cacgacggca tccagccacc tcgagaacga ggcactctgg tcggccacga acgcgacgat
    34501 gaacaccgac tgcaccccgg cctggatgac gagcagctgg ccggacgaca cgacgacggc
    34561 gatccccatg gcgagcacga cgacgagggc catctgcacg gcgccggagc cgagccagac
    34621 gacgatgagg tctccgacga ggatcccgat ggcgaccccg accgtcacct cgacgatgcg
    34681 tcgcagccgc tggccgtagg tgaggccgag acacaggatc gccgtcaccg gcgcgaagaa
    34741 ggggttgacg tgcccgagga cctcggaggc caccagccag gcgacgaaca cggagacgcc
    34801 gatctggagc agcagcaccc agcggtccgc aagccggtcg aggcctcggg ccagggccgg
    34861 ccgggcatcc gggaacctca tcgctcgccg gtcgagagga cgaggtgggc ctgctgccag
    34921 gggcgggccg cctcgtacac ggcatcctcc tggacggccc agcgctgcca gtgcggggcg
    34981 tagacgtctc cgtcgcggct gagggcgcgg cggcgccgca cggcatccgg cgcctcgacc
    35041 cagacccgca cggccgcgtg ctggagcgcg ggcgggacga gggcgccgca cccctcgagc
    35101 acgaggtgcg cgctcgcggc gacgtcgatg gtgcgaccgg ggcgggaccg catccagtcc
    35161 caccgctggt acgcggcgct ccccccgcgg gcgaggggtt cgaggacctg ttcggtcacg
    35221 aggtggaccc cggccgagag cccgtcccag ccgggaaaga tgtcgtccag gtgcacgatc
    35281 gggcacccga gggcgtcggc gacggcagtg ccgagcatcg tcttgcccga tccgctgcga
    35341 ccgtcgatgg cgaccacgac ggtcggtccg gccacgggtg cggcatctgc ggccaccgcc
    35401 agcaccgcgg cgacatgggc tgggtcagcc acgtcggcat gctcggcggg cggctggctc
    35461 atggcggcga ctctagtgct ggcaccatgg ggcgcatgtc cgtggccgtg cgcgtgatcc
    35521 cctgcctcga cgtcgacgac ggtcgggtcg tcaagggcgt caacttcgag aacctgcgcg
    35581 atgccggtga ccccgtcgcc ctcgcgcggc gctacggcga gcagggcgcc gacgagctga
    35641 cgttcctcga cgtcaccgcg agcagtggtg accgcgcgac gatgttcgac gtcgtcgccc
    35701 gcactgccga ggaggtcttc atcccgctga ccgtcggcgg gggagtgcgc accgtcgacg
    35761 atgtcgaccg gctgctgcgg gcgggtgccg acaaggtcgg cgtcaacacc gccgccatcg
    35821 cccgccccga gctcatcgcc gagaccgccg accggttcgg tgcccaggtg ctggtgctct
    35881 ccgccgacgt ccgtcgacgc cgcgacgagc acgggatgcc gtgcggcggc ttcgaggtca
    35941 ccacccacgg tgggcgcacg cgaaccggca tcgacgccgt gcagtggtgt gttcgggccg
    36001 ccgagctcgg ggcgggcgag atcctgctca actcgatgga cgccgacggc accaaggacg
    36061 ggttcgacct cgagctgatc cgcctggtgc gcgccgaggt gaccgtgccg gtcatcgcct
    36121 cgggcggtgc cggtcgcgtc gagcacttcg cccccgcggt gcgggccggg gcggatgccg
    36181 tgctcgcggc cagcgtcttc cacttcggtg agctgaccat cggccaggtc aaggacgcac
    36241 tgcgcgccga cgggctcccc gtccgctgag cgcccctgct cgggcgcggt gctcagctga
    36301 tgccgagcat ggcggttcgc agggcctcga cgtcggcggg tgtgccgtcg aggtcgacgg
    36361 acgccacgcg gatccgccca taggcgagga gcagcagctc gaccggagca cccgtcaccg
    36421 tgaccaccgg tgcgtcgggc cccgcgatga tcggctccgc gccgggcgcg acgaggcgta
    36481 caccgacggg ggcgcggcgg aacatcaggc gcgccgactt ccgcagcgcc gcgtggaccg
    36541 ctcgctcggt gcgctcgggc acctcccggc gcgggccgac cgtcccgtca ccacgcagga
    36601 cgtcctcgtg gtgcacgacg agctcgacgg tgttgacgag cgcgtcgacc cgctcgatcc
    36661 gcgtcggcga ccaccacggt ggcccggagc gcacggtggc gacgagcgac tcccagtcgc
    36721 cctccgccac agcccgctga ccccgctcca gccgtccggc cagggccggg agcaggatgc
    36781 cgggcgcgag gtcggggcgg cgctctcgca cgacgaggtg cgcggcgagg tcgcgggccc
    36841 gccacgggtc gcagagggtg ggcgcgtccg ggccgagctc gaggaaggtg tcgcacaggg
    36901 cctcgcgctc ggcctgcacc accggcagat gggtttccga cgtcatgacg gccattgtcg
    36961 accacgatcc tgcatccccg gtgccttgag ggcgctccgg tgtcgctggg tgcaggtcga
    37021 gcgagcgcaa catgccgtgc ggagggaacg ggcttcggcg tgttgcgctc actcgacctg
    37081 cggcgccgtc aaagaccgca acgcccggat gccgtccgca ccggtccgcg acccggccgc
    37141 cgcaccggga ggcccaccca ccacggcaca atgagcgggt gcgcacgccc ccctttctcg
    37201 acagtgacct cgaccccgcc atcgccgccc gcctcacgcg cgacgccgac ggcctcgtgg
    37261 cggcgatcgt gcagcagcac gacaccggtg aggtgctcat gctcggatgg atggacgacg
    37321 aggccctgca ccgcaccctg acccaggggc gggtcacgtt ctggtcgcgc agccgcagcg
    37381 agtactggcg caagggcgac acctccggcc acgtccagca cgtccggtcg gtcgcactgg
    37441 actgcgacgg cgacgcgctc ctcgtgcgcg tcgaccaggt cggcgcggcc tgccacaccg
    37501 gggaccacac gtgcttcgac gccgcagacc tcggtgccgt ggtcggggac ggcgcgtgag
    37561 cacggcatcc gagagcggtt ccgccgaccg cgcgacgacc ccggacctgg cctacggcca
    37621 gacctggccc aacctcgacg tcttcggcgt gctcgcccgc gaccgccggg tcatccccgt
    37681 cgtgcggcgc ctcatgggcg atgccgagac cccggtcggc gtgtaccgca agctcgcggg
    37741 ggaccggccg ggcaccttcc tcctggagtc ggccgagcac gggggagtgt ggtcgcgctg
    37801 gtcgatcgtc ggcgccgcga gccgggccac gctcaccgag cgcgacggtg cggcccactg
    37861 gatcggtgaa ccaccggtcg gcgtgccgac ctccggactg gccactgacg ccctggccga
    37921 gaccatgcgg gtcctcgcga ccgagcccat cgcgggtctg ccgccgctca ccgggggcat
    37981 ggtcggcgcc atcacctacg acgccgtgcg tcgctgggag cgcgtgcccg ccaccgcccc
    38041 tgacgagctc gacctgcctg agctggccct catgctcgcg accgacctgg cggttctcga
    38101 ccacgccgac gggtcgatcc tgctcgtggc caacgcgatc aactacgacg gcaccgacga
    38161 gcgggtcgaa caggcgtggg cggatgccgt ggctcgcctg gacgacatga cgcgcgccct
    38221 cgcggccccc gcgccgagca cggtcgccgt cgtcgacacg gcatccgtgg acgcggcgat
    38281 ggccacggtc acctcgaaca ccacccgtga gcgctacgag gaggtcgtcg agctcgccaa
    38341 ggaggacatc cgcgccggcg aggtcttcca ggtcgtcatc agccagcggt tcagcctcga
    38401 ctgcgcggca gatccgctcg acgtctaccg ggccctgcgg gcgagcaacc ccagcccgta
    38461 catgtacctg ttccgactgc cgcaccccga cggctcctcg tacgccgtcg tcggcagcag
    38521 ccccgaggcc ctcgtgcgcg tcacaggcga ccgggcgatc acccacccga tcgccggatc
    38581 gcgcccgcgc ggcaagaccc ccgaggacga cgtgcgcctg gccgacgagc tgctcgccga
    38641 ccccaaggag cgctccgagc acgtcatgct cgtcgacctc ggccgcaacg acctccagcg
    38701 ggtctgccgc cccggcacgg tcgacgtcgt cgagttcatg gacgtgcggc gctacagcca
    38761 cgtcatgcac atcgagtcca ccgtcgtggg ggacacccgc ccgggggtca gcgcctacga
    38821 cgtgctggtg tcgaccttcc ccgccggcac cctctcgggt gcgccgaagc cgcgcgccct
    38881 ggcgctcatc gagcagtacg aaccctcgcg gcgaggcgtc tacggtggcg tcgtcggcta
    38941 cctcgacttc cacggtgacc tcgacatggc catcgccatc cgcaccgccg tcgtcaagga
    39001 cggtgtcgcc cacgtgcagg ccggcgccgg catcgtggcc gactcggtcc cggcccgcga
    39061 gcacgaggag tgcgtccaca aggccaccgc ggccctgcgt gcggtcgcga cggccgcggc
    39121 gatgcggacc gtgacatgag ccggctgtcc agcaagggcg tcgtcgtcct cctcgccctg
    39181 ctcggtgccg gtggtctgct cgtcagcggc ttccgtccgt gggtgaccgg ggccgtcgac
    39241 gacgccgtcc tcggggcgac ccgcatcagc gccaccggcg cgcaggtggc cccgggcctg
    39301 agcgccgtcg ccctgtcgat cgcggccgcg gccatcgccg ccgtcgctgc cggccgcatc
    39361 gcgcgggtgg tcgccctggt cgcctatgcg gcctcggtcg tcatcgccgt gatcctcacg
    39421 gtgcgggtcc ttcgggaccc cgaggccgtg ctcggtccgg tcgccgcctc acgcgtcggc
    39481 cgcaccggca gcatcgagaa cgtcgccgac cccacggtgt ggccctgggt ggctctcgcc
    39541 gcctgcgccg tcgccctcct gggcctggtc ggcgcggtgc gcggcctgcg ggtctggggc
    39601 gggccgtcct cgcgctacga ggtggcggat gccggtggcc cggtcgccgg tccgcgcggc
    39661 gagcgcgtcg cgagcgcctg ggacgagctc agcgcggggc gcgaccccac cgatgtgggt
    39721 cccgaccagc ggacctgaca gaatcaccgg tgagccaccg ggccccgagt gtgggccccg
    39781 cacgaatgga gacgacatgg acgagcacga ggaccacggg cacagcgtcg ccgcctggac
    39841 ggcagtgatc atctgcatca tcgcgtcggc gatcatgtcg ctcgcggtgg tgttcccgaa
    39901 ccgctggctg ttcgtcggtg gtgtcgtcct ggccgtcgtc ggtgtcgtcg cggggaaggt
    39961 gctgtccatg gctggttacg gcgctgccgc gcgcgacccc gaacaggcac cctcgatcca
    40021 ctgatgggtg acgccaccgc gggcaccgtt ctcgacgcga tcatcgcggg ggtgcgtgag
    40081 gacctcgccg ttcgcgaggc ggcccgcccg caggccgagg tcgagcggga cgccctcgcc
    40141 gcaccgccgg ccctcgacgc cgaggaggcc ctgcgccggc ccggcctgtc cctgatcgcc
    40201 gaggtcaagc gggccagccc gagcaagggt gagctctccg acatccccga cccggctgcg
    40261 ctcgccgcgg cctaccaggc tggcggtgca tccgtggtct cggtgctcac agaggcacgc
    40321 cggttcggtg gcagcctcgc cgacctcgac gcggtgcgcg ccgcggtcac gatcccggtg
    40381 ctgcgcaagg acttcgtggt caccgactac caggtgtggg aggcccgcgc ccacggggcc
    40441 gacgtcgtcc tgctcatcgt cgctgccctc agcgacgagc agctcacggc cctgctctcg
    40501 cgcgtgcgtg aactcgggat gaccgcgctg gtcgaggtgc acgacgaggc cgagacccag
    40561 cgggccgtcg acgccggcgc gaccgtcatc ggcgtgaacg cccgcaacct caagaccctg
    40621 gacatccacc ccgagacgtt ctcgcggctc gtccccctga tcccccccgg ctgcgtcacc
    40681 gtcgccgagt cggggatcgc cgacgccgcc gacgcaggtg cctacgcccg ccagggcgcg
    40741 gacgccgtcc tggtcggcga ggcgctcgtg cgcaccggtg acccgcgcgg cggtgctgcc
    40801 gcgatggtcg cagcgggcgc catcaccgac cgagaggccg agcagtgacc gacacccaga
    40861 ccgacgacgg catccgcgac ctcaccccgc acgagctgcc gcgacccggc cggttcggcg
    40921 agttcggtgg tcgctacgtc cccgaagcgc tggtcccggc gctcgaccag ctcgacgagg
    40981 cccgccagaa ggcggctgtc gaccccgagt tccaggccga gctggctcac ctgcacgcca
    41041 cctacaccgg gcgaccgagc atcatcacgg aggtcccgcg gttcgcggcg cactgtggtg
    41101 gggcacgggt catcctcaag cgtgaggacc tcaaccacac cgggtcccac aagatcaaca
    41161 acgtcctcgg ccaggccctg ctgaccaaac gcatgggcaa gacccgggtc atcgccgaga
    41221 ccggcgccgg gcagcacggt gtcgccacgg ccacggccgc cgccctgctg gggctggagt
    41281 gcaccgtcta catgggcaag gtcgacaccg agcgccaggc gctcaacgtc gcccgcatgc
    41341 gcatcctggg cgcggaggtc atccccgtcg agcacggcag cgccaccctc aaggacgcca
    41401 tcaacgaggc gttgcgcgac tgggtcacca acgtgcagtc cacgcactac ctcatcggca
    41461 ccgtcaccgg cccgcacccg ttccccgaga tggtgcgcga cttccacaag atcatcggcg
    41521 aggaggcgcg agcgcaggtt ctcgacctca ccgggcgtct gccggatgcc gtgatcgcgt
    41581 gcgtcggcgg cggatcgaac gccatgggca tcttccaccg cttcatcgag gatgccgggg
    41641 tgcgcctgat cgggatcgag gccgggggag aggggatcga gtccgggcgg cacgccgccc
    41701 gcttcgcctc ggcgtcgccc ggggtgctgc acggcgcgat gagctacctc atgcaggacg
    41761 acgacggcca gaccgtggag tcgcactcga tctccgcggg gctggactac ccgagcgtgg
    41821 gaccggagca ctccttcctg cgggacagcg ggcgggccga gtaccgctac gccaccgacg
    41881 accgggtcat ggaggccttc tcgctgctca gccgcaccga gggcatcatc ccggccatcg
    41941 agtccgccca cgccctcgtg ggggccatgg aggtcggccg ggagctgggg cccgacgcgc
    42001 tgctgctcgt caacctctca ggccgcggcg acaaggacat ggagaccgcc gcgcggtact
    42061 tcgggctgct cgacggggag cgggcatgag cacggtcgtg aacggtgtcg gtgagatcct
    42121 ccagcgctgc cgcgaggaag ggcgcgcggc gctcgtcggg tacctgccgg tgggcttccc
    42181 caccgtcgag ggctcgctcg aggcgatgcg cgtcctcgtg gaggccggct gcgacatcgt
    42241 cgaggtcggt gtgccgtaca gcgaccccct catggacggg ccggtcatcc agcacgcggc
    42301 gagcaccgcg ctcgcaggcg gggcccgcgt cgacgacgtc ttccgtgcca cgcaggcggt
    42361 gagggatgcc ggtggcgccg ccctggtcat gacgtactgg aacctcgtgc tgcgccgcgg
    42421 ggtggagcgc ttcgccgccg acctggcatc ctccggaggc gctggtctca tcacgcccga
    42481 cctcatcccc gacgaggcgg gcgagtggat cgccgcgagc gatgcgcacg ggctcgaccg
    42541 ggtcttcctc gtggcaccgt cgtcgacgcc cgagcgactc agtgcggtca ccggcgcctg
    42601 ccgcggcttc gtctacgccg cgtcgacgat gggcgtcacc ggcgcccgca cgagtgtcga
    42661 ctccgacgcc cgtgggctcg tcgagcgcac ccggtccgtc accgacctgc cggtctgcgt
    42721 ggggctcggt gtctccaccg gcgcccaggc cgccgagctc gccgcgtacg ccgacggggt
    42781 catcgtcggc tccgccctcg tccgcacgct caccgggccg ggggaactct gtgaccggct
    42841 cgaggcgttg cgcgctctga cgaaggaact ggctgacggt gtgaggggtg gtcgcggatg
    42901 agcacggcca ccgaccccac cctccacgag gtgtcgccga cgcccgagcg cggtgagcgg
    42961 ctcaaggacg tcatcggcct gctggcccgc ctcttcctgg gcgccgtcct catctacgcc
    43021 ggcgcggtca aggtcggcaa gccgctgacg tccgagcgcg ccgtgcaggc cttcgagatc
    43081 ttccccacgg acctcgccgg ctacatcggc ctggccctgc ccttcgtcga gatcctgctg
    43141 ggtgcgctgc tcatcctggg cctgttcacc cggccggtgg ccatcgtctc gaccctgctc
    43201 atggtcgcct tcatcatcgg catcgcccag gcgtgggcga ggggcctgac catcgactgc
    43261 ggatgcttcg gtggcggcgg gcaggtggcc gcgggtgaga cgaagtacgg caccgacatc
    43321 gcccgcgatg cgaccttcgc ccttgccggg gcctggctgt ggtggcgccc gcgctccctg
    43381 gcctcccttg accgtctgct gttccgctaa accacgagga gactgaccac catggcgaag
    43441 aactccgcca aggccccgga tcggggtgcc gccaacgcgc gcgcggcaaa gatccaggct
    43501 gcccagaagt ccgccggggg cggggcgaac aagatcgtcg tcgccacggt cgtcgcgatc
    43561 gtcgcgatca tcgccacggt gggtggcgtc gtctggagcc agatgtcgaa ggagaacgac
    43621 gtcaccggag ggggcaacgc ccttcccgcg ggggtcagcg agctgggcga ggggtacccg
    43681 gccttccagg acgtcacgcc cgtcgacggt gcacccaccg tcgacctcta cgtcgactac
    43741 cagtgcccgg cgtgcgccat gttcgagcag gccttcgggc cgacgctgag cgagcaggcc
    43801 gccgacggca agatcaagct ggtctaccac gtgaagaact tcctcgacga caacctcagg
    43861 aacgacagct ccacgcgctc cgcgaacggg gccttctgcg cctcggacgc cggcaagttc
    43921 caggagtaca gtgaccagct cttccccggt cagcccgagc gcgagggtga tggctggacc
    43981 gatgcccagc tcaaggcatt cgccgagacc gccggtctga gcggcgcgga cctgacgacc
    44041 tgggagtcct gcgtcgccgc aggcaagtac accgactacg tgaactcggt cgagaagcag
    44101 tcgttcgccg atggcgtcaa gggcacgccc accgtcaagg tgaacggtga ggatctcgac
    44161 accggcaccc tggtcaacgc cgagggcacg gccttcgacc cggccaagct cgtcgcggcg
    44221 ctcgaggctg ccggcaagtg atcgcctccc tgcccagccc cacggtcggg gtgtggcacc
    44281 tgggtccgtt cccgctgcgg atgtacgccg tgtgcatcct gctcgggatc gtggtcgcgg
    44341 tgtggctcac cggccgacgc ctcgtcgacc ggggctacga ggctgggcag gcgctcgacg
    44401 tggctgcgta tgccgtgccg ttcggcatcg tcggcggccg gatctaccac gtcatcacca
    44461 cccctgaccc ctactggggt gagggcggca acccggtcga cgcgctgaag atctgggagg
    44521 gcggcctcgg catctggggc gcgatcgccc tcggggcgtt cggcatctgg gtcggctgcc
    44581 ggcaggtcgg tatccgcttc ctgcccttcc tggatgccgc ggtgccgggt gtggcgctgg
    44641 cgcaggcgat cggacgcgtc gggaactggt tcaacaacga gctgtacggc ggccccacga
    44701 cgctgccgtg ggggctgacc atccaccagt gggaccaggg cgccggtcgg gctgtcgagg
    44761 acgcctcggg caaccccgtc gtcctcgggg tgttccaccc gacgttcctg tacgagatga
    44821 tctggctcgt catcctcgcg gtggcgctgc tcgtcatcga caagcggcgt gcgctggccc
    44881 cggggcagct cgccgggctg tacgtcgcgg ggtaccccgt gggccgcatc gtcatcgagt
    44941 tcatgcgcac cgacaaggcc gagctgatcc tcggggtgcg gctcaacgtc tggacgagca
    45001 tcatcgtctt cgccctaggg gtgtggatct actggttcac cggtcgccgc gccgagcgca
    45061 acgctgtccc gcagagcgaa cccgaatccc ggaatgtggg atagcgaaac gcaaatcgcg
    45121 agaccgcgcc gccgggcgat agtcttccgg cacgtggcca gagccgtccg cgctgacctt
    45181 gccgtgcccg gcccctcgag ggcccacgac tgaagaggac ggtgatgcga cgtgaccgtc
    45241 gcccgcttct ccgcccagcc caccgacacg ggcctgtacc gccgcgagca cgagcacgat
    45301 gcctgcggtg tggccctcgt ggcgacgatg cggggcagtg ccggccacga catcgtcgcg
    45361 cacgccctcg acgcgttgcg caacctcgac caccgcggcg ccaccggcgc cgacccgctg
    45421 gtgggtgacg gcgccggcat cctcacccag gtccccgacg cgttcttccg cgccgtcctg
    45481 gatgccgacc tccccgaggc gggcgcctac gccgtcggca tggcctacct gccccaggac
    45541 gacgacgagc gcgccctggc cgagcagcgg atcgccgaca tcgcggtgga cgagggcctt
    45601 cgcgtcatcg cctggcgtga cgtgcccgtg acccccgacc tcgtcggcca gtccgcccgc
    45661 gagtgcatgc cggtcttccg tcagctgttc gtcacctccg agcaggggct tcgcggcatc
    45721 gagctcgacc gcgtggcctt ctgcctgcgc aagcgcgccg agcacgaggc cgaggtctac
    45781 ttcccgtccc tgtcgtcccg cacggtcgtc tacaagggca tgctcaccac cggtcagctc
    45841 gagccgttct tccccgacct gtccgacgag cgcttcgcct ccgagctggc tctcgtgcac
    45901 tcgcgcttct ccacgaacac cttcccgagc tggccgctcg cgcaccccta ccggttgatc
    45961 gcgcacaacg gtgagatcaa caccgtcaag ggcaaccgca actggatgcg ggcgcgcgag
    46021 agccagatca gctcgaccgt catccccggc gacatcgagc gggtcttccc gatctgcacc
    46081 cccggtgcct ccgactcggc gacgttcgac gaggtcctcg agctgctcca cctcgggggc
    46141 cggtcgctgc cgcacgccgt gctcatgatg atccccgagg cgtgggagaa ccacgagtcg
    46201 atggaccccg cgcggcgcgc cttctacgag ttccactcca cgttcatgga gccgtgggac
    46261 ggcccggcct gcgtcacctt caccgacggc accctcatcg gtgcggtgct cgaccgcaac
    46321 ggcctgcgtc cgggccgcta ctgggtcacc gacgacgggc tcgtcgtgct cgcctccgag
    46381 gcaggcgtcc tggacatcga gcccgagcgg gtggtgcgcc gtgggcggct gcagccgggc
    46441 cggatgttcc tcgtcgacac cgcggcgggc cgcatcatca gcgacgagga ggtcaagtcg
    46501 ggcctggcgg gcgaacaccc ctatgaggag tggctgcacg cgggcctgct cgagctgtcc
    46561 gacctgcccg agcgtgagca catcatccac acagccgcct cggtggcgag gcgccagcgc
    46621 accttcggct acaccgagga agagctgaag ctcatcctcg cgccgatggc ctccgccggc
    46681 ctcgaggcgc tgggttcgat gggcaccgac accccgctcg ccgtgctgtc cgaccggccg
    46741 cgactggtct tcgactactt cagccagctc ttcgcacagg tcaccaaccc cccgctcgac
    46801 gcgatccgcg aagagctcgt cacctcgctc gggtcctcga tcggccccga gggcaacgtc
    46861 ctcgacgcct ccccggcgca cgcgcgccag gtggtgctgc ccttcccggt catcgacaac
    46921 gacgagctcg ccaagctcgt gcacatcaac gccgacggtg acctgcccgg ctacagcacc
    46981 gtggtcgtca agggcaccta cgacgtcaac ggcggcgccg cggccctgcg agcccgcctg
    47041 cacgagatct tcgccgaggt gtccacggcg atcgggcagg gcgcccggtt cgtcgtgctg
    47101 tccgaccgcg actccgaccg cgacctggcg cccatcccgt cgctgctgct cacctccgcg
    47161 gtgcaccacc acctcatccg cgagaagacc cgcacccagg tcggcctgct cgtcgaggcc
    47221 ggcgacgtgc gcgaggtgca ccacgtggcg ctgctcgtcg ggttcggcgc agcggcgatc
    47281 aacccgtacc tcgccatgga gaccgtcgag gacatggtcc gcagcggagc catcaccgcg
    47341 gtcaccgagg agaaggccgt cgccaacctc atcaaggccc tgggcaaggg ggtcctcaag
    47401 gtgatgtcca agatgggtat ctcgaccgtc gcctcctacc gcggcgccca ggtcttcgag
    47461 gccatcgggc tctcccagga cctcgtcgac gagttcttca ccggcaccgt cagccagctc
    47521 gggggggtcg acctcgacgt catcgccgcc gagaccgcgg cgcgccacgc catggcctac
    47581 ccgcccgacg gcatccgcca ggcccaccgc aagctgctcg tcggtggtga gtaccagtgg
    47641 cgccgcgagg gtgagccgca cctgttcgac cccgagaccg tcttccggct ccagcacgcc
    47701 acccgggccc gtcgctacga cgtcttcaag cagtacaccc gtcgcatcga cgagcaggcc
    47761 gggcgcctga tgaccctgcg cggcctcttc gagctcgggg gagcgcagcc gcgcccgccc
    47821 gtgccgatcg aggaggtcga atccgtcgcc gagatcgtca agcgcttcaa caccggtgcg
    47881 atgagctacg gctcgatcag catggaggcg cacgagaccc tggccatcgc gatgaaccgg
    47941 ctcaaggccc gctcgaacac gggtgaaggc ggcgaggacc ccgagcgact gatggacccg
    48001 cagcggcgct cggccatcaa gcaggtcgcc tcaggccgct tcggcgtcac ctcgctgtac
    48061 ctgacccacg ccgacgacat ccagatcaag atggcccagg gtgccaagcc cggcgagggc
    48121 gggcagctgc ccggtcccaa ggtctacccg tggatcgcca agacacggca ctcgacgccg
    48181 ggcgtcggcc tgatcagccc gccgccgcac cacgacatct actccatcga ggacctcgcg
    48241 cagctcatcc acgacctgaa gaacgcgaac ccgcaggccc gcatccacgt caagctcgtc
    48301 tccgaggtcg gcgtcggcac ggtcgcggcc ggcgtcagca aggcacacgc cgacgtcgtc
    48361 ctcatctccg gccacgacgg cggcaccggc gccagcccgc tgaccagcct caagcacgcc
    48421 ggcggtccgt gggagcttgg cctggccgag acccagcaga cgctggttct caacggtctg
    48481 cgcgaccgca tcgtcgtgca ggtcgacggt cagatcaaga ccggtcggga cgtcgtcatc
    48541 gccgccctgc tcggtgccga ggagttcggc ttcgccacgg cgccgctcgt ggtctcgggg
    48601 tgcatcctca tgcgggtgtg ccacctggac acctgtccgg tcggaatcgc cacccagaac
    48661 cccgagctgc gcgcccgctt ctccggccag ccggagttcg tcgagacctt cttcgagtac
    48721 atcgccgagg aggtccgcga gcacctcgcg gcgctgggct tgcgcagcat cgaggaagcc
    48781 atcgggcagg tccagtacat cgacgcctcc aaggcgatca accactggaa ggcctccggc
    48841 ctggacctcg ccccgatcct ggcgcgcgtg gagagcccgg acggctccgg catccgacac
    48901 accatcgggc aggaccacgg cctcgagaag gcactggacc acgagctcat cgctgcctcc
    48961 cgcgacgcga tcgaccacgg caccccggtc accgcccggt tccgcatccg caacgtcaac
    49021 cgcaccgtcg gcacgatgct cgggcacgag gtgaccaggg cgcacccgaa cgggctgccc
    49081 gacggcacca tcgacctcac cctgaccggc tcggccggcc agagcctcgg ggccttcctg
    49141 cccgccggaa tcaccctgcg cctcgagggt gacgccaacg actacgtcgg caaggggctc
    49201 tccggtgggc gcgtcatcgt ccgcccggca ccggcatccg ccctcgtcgc cgaggacaac
    49261 gtcatcggcg gcaacgtcat cggctacggc gcgacctcgg gtgagctctt cctgcgcggc
    49321 cgggtcggcg aacgcttctg cgtgcgcaac tcgggtgcca ccgcggtcgt cgagggcgtc
    49381 ggtgaccacg gcctcgagta catgaccggc ggcaccgcgc tcatcctcgg gcccaccggg
    49441 cgcaacgtcg ccgccggcat gtccggtggc gtcgcctacg tgctcgacct cgacccggcc
    49501 cacgtcaacc gtgagctcgt cgacctcggg ccgctgcgcg ccgaggacga actcgtcgtg
    49561 cacgacctgc tcgagcgcca ccaccggtgg accgactcag cggtcgcagc ccgactcctc
    49621 gccgactggg actccgcccg ctcggcattc acccttgtcc ttccccgctc ctaccagcgc
    49681 gtcctcgacg tgcgggccaa ggcggcggcg gaggggctgg accccgacgg cacccaggtg
    49741 tgggagcgga tcatggaggc ctcccgtggc tgacccccaa ggtttcttgc gcacgcgtga
    49801 gcgtgaactg ccgcgacgtc gccccgttcc ggtccggatc aaggactgga aggaggtcta
    49861 cgaggagcag ccggtccccg agctccagcg gcaggccggg cgctgcatgg actgcggcat
    49921 cccgttctgc cacagcggct gcccgctcgg caacctcatc ccggagtgga acgacctggc
    49981 atggaagggc gagtggcgcc aggccatcga ccgcctgcac gcgacgaaca acttccccga
    50041 gttcaccggt cggctctgcc cggcgccgtg tgaagcggcc tgtgtcctcg gtatcaacca
    50101 gcccgccgtg acgatcaagc aggtcgaggt cagcatcatc gaccgggcct tcgagaacgg
    50161 cacggtcacc ccggaggcgc ccgagcggct gtccggcaag accgtcgcgg tcatcgggtc
    50221 cggcccggcc gggctcgccg ctgcccagca gctgacccgc gccggccaca ccgtggccgt
    50281 ctacgagcgc gccgacgcgc ccggaggcct gctgcgctac ggcatccccg agttcaagat
    50341 ggagaaggcg gtcctggacc gccggatcgc ccagatgaag tccgagggca cccggttccg
    50401 caacggggtc gagatcggca aggacctcac ggccgccgac ctgcgcaagc ggttcgacgc
    50461 cgtcgtcatc gcgaccggcg ccaccgtgcc gcgcgacctg cccgtccccg ggcgtgagct
    50521 cgacggggtc atgcaggcca tggacttcct gccgcccgcg aaccgggtgg cccgcggcca
    50581 gcacgtcgac ggccaggtga cggccgaggg caaggacgtc atcgtcatcg gtggtggtga
    50641 caccggcgcc gactgcatcg gcacctcgca ccgccagggt gcccggtcgg tgaccagcct
    50701 ggagatcatg ccgcagcccc cgggcgagcg gcccgggaac cagccctggc ccacctaccc
    50761 gatgaccttc cgggtcgcct cggcccacga ggagggtggc gaccgcgtct acgccgtgtc
    50821 gacgagcgag atcgtcggtg acgagcacgg tcgcgtcgcc gccctgcgcc tgcacgaggt
    50881 gcgcatgggt gagaacgggt tcgagaaggt cgagggcacc gagcgtgagc ttccggcgca
    50941 gctggtcctg ctcgcgatgg gattcctcgg cccgcagcag gacctgctcg accagttcgg
    51001 ggtcgagcgc gacgcccgca ccaacgccgc ccgcgacgac gcgtacatga ccaacgttcc
    51061 cggcgtattc gtcgccggcg acgccggtcg tgggcagtcg ctgatcgtct gggccatcgc
    51121 cgagggccgg tccgcggcca acggggtcga cgcctggctg atgggcaagc cctcccgcct
    51181 gccgcgcccg gtcaacccca cggatcgtcc cctgatggcc tgaggccacc cgaccccggg
    51241 gggaccacgc ggatgccgtg tggtcccctt ccgggatgtg gcgcgcgtca ccggcggaag
    51301 cgtttgcacc actaggctgt acccatgcgt cgcgccaaga tcgtctgcac cctgggtccc
    51361 gccaccagct ccgccgagaa cctgcgggaa ctcgtccgtt ccggcatgga cgtcgcccgg
    51421 ctgaacctca gccacggcga ccacaccgac cacgagcgcg tctaccatga cgtgcgcagg
    51481 gccagcgacg ccatggggcg cgccgtcggc atcctcgtcg acctccaggg cccgaagatc
    51541 cgcacggccc gcttcgagaa cgggccgatc gagctggtgc ggggggcgtc cttcaccatc
    51601 accacccgcg acgtgccggg caacgaggtc gaggtcggca ccacctacgc cgggctgccg
    51661 ggcgacgtcg ccatcggcga ccgcatcctc atcgacgacg gcaagctggc cctcatcgcg
    51721 accgaggtca ccgataccga cgtcgtctgc caggtgatcg agggcgggat gctctccaac
    51781 aacaagggca tcaacctgcc gggcgtcgcc gtgtcggtgc cggcgatgtc ggagaaggac
    51841 aaggacgacc tgcgctgggc cctgcgcatg cgggtggaca tgatcgcgct gtcgttcgtc
    51901 cgcagcgccg aggacatcct cgacgtccac aagatcatgg acgaggaggg tctgcgcctg
    51961 cccgtcatcg ccaagatcga gaagccgcag gcggtcgaga acctcgacga gatcatccgg
    52021 gccttcgacg gtgtcatggt ggcccgcggt gacctcggcg tcgagatgcc cctcgaggag
    52081 gtccccctcg tgcagaagcg cgcctgcgag atcgcgcggc gccgcgccaa gcccgtcatc
    52141 gtcgccaccc aggtgctcga gtcgatgatc gacaacgccc gccccacccg cgccgaggcc
    52201 tcggatgccg cgaacgccgt cctcgacggt gccgacgcgc tgatgctctc gggggagacc
    52261 agcgtcggca agcacgcctt cgcggcggtg cgcacgatgg cccgcatcat cgagaacacc
    52321 gaggaccacg ggctgcaccg catccgtccg ctcggcaccc gcccgagctc caagggcggc
    52381 gcggtcaccg cagccgccgc cgacatcggt gagctgctcg gggtcaagtt cctcatcacg
    52441 ttcaccgaga gcggcgactc cacgcgccgt ctgtccaggg tgcgcccgcg catcccgatg
    52501 ctcgcgttca cccccaacca gtggacccgc agccagctcg ccctgacctg gggtgtcgag
    52561 accttcctcg tcggcaaggc caaacacacc gacgagatgg cgatgcaggt cgacgaggtg
    52621 ctcctcgccg ccggccgggt tctcgagggc gacaccgtga tcatcgtcgc gggctcgccc
    52681 cccggcatcc cgggctccac gaacgcgttg cgcgtgcacc gcgtcggcga cgccaagaac
    52741 cgcgtggccc cggcctacag cgagctctga gcccgagcgg cgaccagcca gcatgccgca
    52801 cgccggtatg ctggtcacgc cccggccggg gtggtggaac ggcagacacg gagcactcaa
    52861 aatgctctgc ccgcaagggc gtgcgggttc gagtcccgct cccggcacat gagtgagacc
    52921 agtcctgtcc cggttgcccc cgcggctgcg ccgtccggtc cgaccaagcg cgtgctcgtc
    52981 gccgaggacg aggccatcat ccggctcgac ctcgccgaga tgttgaccga agccggctac
    53041 gaggtggtgg gtcaggccag cgacggtgag caggctgtcg ccctggccgc gtcgttgaag
    53101 ccggacatcg tcatcatgga cgtcaagatg ccggtgatgg acgggatcag cgcggccgaa
    53161 cagatcggca aggagcggct ctgcccggtc gtcatgctca ctgcgttcag ccagaccgag
    53221 ctcgtcgagc gggcgcgtga cgcgggcgtc atggcctaca tcgtcaaacc gttcacggcc
    53281 agtgacgtca cgcccgccct ggacatcgcg ttgtcgcgct gggccgagct gaagaccctc
    53341 gaggccgagg tcgccgacct cggcgaccgg ttggagaccc gcaaggccgt cgaccgggcc
    53401 aagggggtgc tcatgaccaa gctgaagatc accgaggccg acgcgttccg gtggatccag
    53461 aagaccgcga tggaccgccg gatgagcatg cgcgaggtcg ccgacgccgt cgtcgccggc
    53521 atggagaagg cctgacccca gcgcttcgtc agcgcagcca gatcaccagc tgggtgagca
    53581 gcggcgcgac gatcagggcc ggcagcatgt cccccacggg gatgtcccgg atgcgcagca
    53641 gccgcaacgc gatcccggcg agcatcagcc caccagtggc ggtgagggcc gagatgtggg
    53701 catcgggcac gaggtcgccg agcagcaccc cgacgacggt gagcgcaccc tgcacgacga
    53761 gcaccgacac ggctgagagc agcaccccca cgccgaaact ggccgcgaac gccagggcgg
    53821 cgaaaccgtc gagcaccgcc ttgagcgcca gctggtcgat gccgcggccg agaccgtcgt
    53881 tgagggcgcc gaggatcgtg agcgggccca cgcagaacag cagggatgcc gtgagccacc
    53941 cttcgatgaa ccgctcgcgg gatgcgtggt cgcttccccc ctcgatgctc gaatccttcg
    54001 accgacgcga cacccagccc tggatcgttc cggcgagccc ctcgagacgc tcctcgatgc
    54061 gcagcatcga tccggtgatc gagccgatga gcaacgaccc gaggacgatg agcaccggga
    54121 agccggaccc gaccgcgtcg ctgagggcga cgtcggtgac ggccatcgct gacaggcctg
    54181 ccacgagcag ggtgaccagg ccgagcacat cggtcacgac cgagcgcgtg tgctcgcgca
    54241 gacggtgccc gacggccatc ccgagacccg ccccgatgag gacggtgagg acgttgaggg
    54301 cggtcccggc accgggaaag agcgaagtca ccgccgcagc ctacgagggt cgtccacagg
    54361 tgctcgtcgg ggggttccgt ccgggctcgc cggggtctag ggtcggtggc cgcaggggta
    54421 acctctttgc ctgtggcgca ctcgcgcccg ctgcgatgtc cgaggaggcg atgtggccag
    54481 acgcacggtg gatgtgatgc ccgtggaccg cgacgacctg cgggacgtca tggggctgtg
    54541 gctggctgcc agggtcgaca gcggttcgag caaggaggcc gctggtcggg ccctcaccga
    54601 cggtcggctc agcacggcgc tgcgtcgacc cggcgtccac tccttcgtcg cccggatgga
    54661 cggcgagccc gtcggctacg ccatcgccag cgagaacccg ttcggcctca gcccccaggc
    54721 cgagctcgcc atcgagcagc tgtgggtcgg gcctgacgca cggcgccacg gggtggccaa
    54781 gtcgctcctc gcggccgtgc tgtccaccgc cgagcggtcc gggtgcgagg tcgtggtctc
    54841 gaacgtgccg acggccagcc gcgacgccaa ccgcttcttc gcccgcctgg gcttcgcgtc
    54901 ggtcatcacc cgtcgggtcg tctccacgag tgcgctgcgt cgcaagctgg ctcctgagag
    54961 tgtcgagacc ggccacgagc tgctgcgccg ccgccgctca ctgcgcagcc gggccttccc
    55021 ggcggcatcc cgtcagttct gacctgacgg tcaggcgccc ggggcggcat ccctgatcag
    55081 gcaggtgatc cggctcgtgc aggtgcgcgc gcccgtgtcg tcggtgacga cgatctcgta
    55141 acacgcaagg gtgcgcccca gggagatggg cgtcgcgacc ccggtgacca gaccggagcg
    55201 ggctgcccgg tggtgcgtgg cgttgatgtc gaggccgacg acggccttgc cgggcccggc
    55261 gtgcagggcc gccccgatcg agcccaacgt ctcggcgagc accaccgagg cgcccccgtg
    55321 cagcaggccg tagggctggg tgttgccctc caccggcatc gtggcgacga gccgctgcgc
    55381 ggacgcctcg atgatctcga tgcccatccg gtgcatcagg gagttcgggt tcatcgcccc
    55441 catcgccttg acaccggccg gcacgtcggt cgggtgctcc ggggtgcggt cgtgggtggg
    55501 ctgcgtggcg tcggggctgt cggtcatggt ggataggctg ccacgcgtga agcttctcct
    55561 cctcgacgga cactcgctgg cctaccgtgc gttcttcgcc ctgcccgcgg agaacttctc
    55621 cacgagcacc gggcagcaca cgaatgccgt gtacgggttc acgtcgatgc tcatcaacgt
    55681 gctgcgcgac gaggcgccca cgcacgtcgg ggtggcgttc gacgtctccc gcaagacctt
    55741 ccgcgccgcc gagtacccgg agtacaaggc agggcgctcg gcgaccccga ccgagttctc
    55801 gggccaggtc ccgctgctgc acgaggtgct cgacgcgctg cgcatccggc acgtcggcat
    55861 cgaggggtac gaggcggacg acgtcatcgc cacgctgacc caccgcgcgg tcgagcaggg
    55921 ccacgaggtc gtcatctgca cgggtgaccg cgatgcgttc cagctggtct ccgacaaggt
    55981 caccctgctc tatccggtgc ggggggtctc cgaggtgtgg cggatggatc cggccgcggt
    56041 caccgagaag tacctcgtgc cccctgagcg ctacagcgac ctcgcggcgc tggtcggcga
    56101 gtccagcgac aacctgccgg gtgtcccggg ggtcgggccg aagaccgcgg ccaagtggct
    56161 cggacagtac ggcgacctcg ccggcatcgt cgcccacgtc gacgagatca agggcaaggc
    56221 cggcgagaac ctgcgggcgc acctcgacgg ggtgctgcgc aaccgccggc tcaacaagct
    56281 cgtcgacgac ctcgacctgc cgctcggcat cgacgacctc cagcgcgcgt cgtgggaccg
    56341 cgaggaggtg caccgggtct tcgacggtct ggagttccgc gtcctgcgtg accgcctctt
    56401 cgccaccttc gacgtcgagg ccgacgaggt agagggcggc ttcgagctcg agggcgaggt
    56461 cgtgcacgcg gacgggctcg cgctgcgctt cggcgagggt gcccacgggc gccaggtgcc
    56521 ccacggcgtc gtcgtcaccg gccactaccg cagcggttcg ggggatgccg gtgccgtcgc
    56581 cttcgcgggt gccgacggcg tcaccgggta cgtcgacctc accaccctca cgtcggagca
    56641 ggacgaggcc ctggcgtcgt ggctcgccga cccgtcggct cccaaggtgc tgcacgacgc
    56701 caaggagcag ctccaggcgt tgtcgacgcg gggtctgacc ctggccggca tcacctcgga
    56761 caccgcactg gcggcgtacc tcgtgcgccc ggaccagcgc tcgtacgacc tcgaggacct
    56821 cgtcgtgcgg cacctcggcc gagagctgaa ggcgcaggag gccgccggcg agcagggcat
    56881 gctcgacttc gcgcccgacg acgacacctc cgaacgtgag gccatggttc gcgcccgcgc
    56941 ggtgctcgag ctctccgagg tgctggggga gcggctcgag gagaccggtg gcaccgacct
    57001 gctgcgcgac ctcgagctgc ccctcgtcga catcctggcg cgcatggagc gcaccggcat
    57061 cgccgtcgac gaggacgcgc tcaccgccct cgaggccgac ttcgccgcga agatgcgcca
    57121 ggcgcagctg gacgcctacg aggccatcgg ccgctcggac gtcaacctcg gcagccccaa
    57181 gcaacttcag gaggtgctct tcgaccagct gggcatgccg aagaccaagc gcaccaagac
    57241 cggctacacc acggatgccg acgcgctcgc cgacctcttc gccaagaccg agcacccgtt
    57301 cctcgaggcg ctgctgcgcc accgagacac cagccgcctg cgggtcaccg tcgaggggct
    57361 catcaagtcg gtggcgcccg accggcgcat ccacaccacc tacatccaga ccatcgcggc
    57421 caccgggcgg ctcagctcga ccgacccgaa cctgcagaac gtgccgatcc gcaccgagga
    57481 ggggcgccgc atccgcgagg tcttcgtcgt cggcgagggc tacgagtcgc tcatgtcggc
    57541 cgactacagc cagatcgaga tgcggatcat ggcgcacctg tcgggtgacg aggggctcat
    57601 cgaggccttc cgcaccggtg aggacctgca ccggttcgtg ggcgcccggg tgttcggggt
    57661 cgagccgcag gacgtcaccg cggccatgcg cagcaaggtc aaggcgatga gctacggctt
    57721 ggcctacggg ttgtcggcgt tcgggttgtc caagcagctg aacatctcca cgggtgaggc
    57781 tcagggcctc atggatgagt acttctcccg gttcggcggg gtgcgtgact acctgcgcga
    57841 ggtggtcgtc gaggcccgtg cgaccggcta caccgcgacg atgctcggtc ggcgccgcta
    57901 cctgcccgac ctggtcagcg acaaccgcca gcgccgcgag ggcgccgagc ggatggcgct
    57961 caacgcgccg atccagggct cggccgccga catcatcaag ctcgcgatga agggggtgga
    58021 tgccgcgctg cgcacggagg ggctggcctc acgcatcctg ctccaggtgc acgacgagct
    58081 cgtgctcgag gtcgcgccgg gggagcggga ggcgctcgag tcgctcgtgc gccgcgagat
    58141 gggtgccgcg gtcgccatgg acgtgccgct ggacgtcagc gtcggggtcg ggcgctcgtg
    58201 gcacgacgcc gctcactagg cgacggctcc cggcccccgg tgaccaaccg attccaggcc
    58261 cccggtgacc atgagggttc acaacaccac ctcgtccaca agaagggctc cgggcgccac
    58321 gttgccacgc ggcccgacca cgcagtcgcg caaggtcgcc ctctcctgca ccgtcgcccc
    58381 tcgcatgacg atggagcccg tgacagtcgc cccgcgaccg atgaccgcgt cgggtccg
//
LOCUS       DAOSOC010000002        51637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_700_length_51637_cov_9.917452, whole genome shotgun sequence.
ACCESSION   DAOSOC010000002 DAOSOC010000000
VERSION     DAOSOC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 51637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51637
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_700_length_51637_cov_9.917452"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PL014_00270"
     CDS             <1..165
                     /locus_tag="PL014_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HOT55552.1"
                     /translation="IAETQEMLDFCAEHGIAATIEVVSAADVNEVWDRVVDGDVRYRA
                     VIDTSTITPA"
     gene            complement(247..1638)
                     /locus_tag="PL014_00275"
     CDS             complement(247..1638)
                     /locus_tag="PL014_00275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT55553.1"
                     /translation="MSSPTLTFWGAAGTVTGSRFLVETAASRVLVDAGLYQGLRELRQ
                     RNWDVLPVDPDSLDGVVLTHAHLDHCGYLPRLVRDGLAAPVICTDQTAELAAIVLRDS
                     ARIQEEDAGYANQAGFSRHRPALPLYDERDVEQALPLLRPVPLHDAVPLTEDVTVTLQ
                     SAGHILGAASALVDAAGSTVLFSGDLGRPDHPLVPRRENPPSADVIVVESTYGDRTHP
                     PRSPDPADDVLAGAIRRTLARGGTVLIPAFAIDRTELVLLRVGELMTSGAVPTVPVYL
                     DSPMAQRALDVYRRYLATVDPGLLPPGWEVDSLGVPDLRIVRNAVESEALNVPRHPCV
                     IISASGMATGGRVVHHLAHQLGDPRNCVVLTGYQAVGTRGRDLLEGAREVKMHGRYVP
                     VRAEVVDDPEFSVHADSDEIIEWLSRIETPPTTLYAVHGEPAASRALADRIARELGWT
                     AVVPRVGERVRLD"
     gene            complement(1780..3330)
                     /locus_tag="PL014_00280"
     CDS             complement(1780..3330)
                     /locus_tag="PL014_00280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT55554.1"
                     /translation="MTDVTSTTAPEAQAAPAPSFSDFPIHPEIVEALAEHNITSPFPI
                     QAMTLPVALSGHDIIGQAKTGTGKTLGFGIPMLNRVVSPRDEAFASMPAAGKPQALAV
                     APTRELAVQVASDLERAGKKRGIRVLTVYGGRAYEPQVEALQRGVEVVVGTPGRLIDL
                     AKQGHLDLGHARIVVLDEADEMLDLGFLPDVEKLLSLTPAARQTMLFSATMPGAVVAL
                     ARRYMSQPTHIRAMGDDQENAHTVKAVEQFVYRAHAMDKVEMLARMLQAKDRGLTIVF
                     SRTKRTAAKVADELADRGFAAAAIHGDLGQGAREQALRAFRNGKVDILVATDVAARGI
                     DVENVTHVINYQCPEDEKTYLHRIGRTARAGNTGIAVTFVDWDDLHRWAMINKVLDLG
                     MPEPQETYSSSPHLFTDLDIPEGSKGRLPRAQQTRAGLGAETLEDLGETGKGAGRRGP
                     SGGRGNERGNDRGDRGRGGDRRSDAARPSSRDGDAPAEGAGRPRRTRNRRRTSGGSGS
                     SASGTTAS"
     gene            3511..4179
                     /locus_tag="PL014_00285"
     CDS             3511..4179
                     /locus_tag="PL014_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HOT55555.1"
                     /translation="MEHMSTEDPAYTAAVSDLLGVLAYGELTAFIRMAVDSDLAPTLA
                     LKSSMAGLAHTEYVQYSQLIEHMGARGIDAEAAMAPFVTPFAAYHERTKPRDWIEGLV
                     KAFVGDGIAKDFYREMSAFVDDETRAVMGLALDDEGQADFVVGVVRDTLKTDRTAAGR
                     LSLWGRRLLGEALSQAQAVAVERDAMSALLVGGGVDLAEVGQMFARLTENHQKRMALM
                     GLTA"
     gene            complement(4275..4547)
                     /locus_tag="PL014_00290"
     CDS             complement(4275..4547)
                     /locus_tag="PL014_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOT55556.1"
                     /translation="MGTLGAIIGTIIFGAVIGMLARLALPGKQQISALLTVALGVAGA
                     LIGSAVWEALGGGPTRGIDWIRWFISIAAAAALVIGYEKVTAKKSV"
     gene            complement(4596..4817)
                     /locus_tag="PL014_00295"
     CDS             complement(4596..4817)
                     /locus_tag="PL014_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HOT55557.1"
                     /translation="MEVTIGVQNVARELTIETDSPTAEVTAAVEKALADGGVLRLTDS
                     RGRSVFVPGSVIGWIQVGDSDKARVGFTP"
     gene            complement(4988..5608)
                     /locus_tag="PL014_00300"
     CDS             complement(4988..5608)
                     /locus_tag="PL014_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT55558.1"
                     /translation="MTSTPMPTRGQRMPRSERRAQLLDAAQAVFVQSGYHAAAMDEIA
                     DRAGVSKPVLYQHFPGKLDLYLALLDKHCDTLESLVRAALEVGGDNEVRVERTVAAYF
                     QFVTSAGAAFRMVFESDLTSVPQVRARLDAVEMNCAEAIAEVIAEDTGADDERALLLG
                     SALAGMAQVAARHWLAQGGDVPEAEAARMISSLAWRGLGSFPKVEA"
     gene            5703..6875
                     /gene="moeB"
                     /locus_tag="PL014_00305"
     CDS             5703..6875
                     /gene="moeB"
                     /locus_tag="PL014_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HOT55559.1"
                     /translation="MSRPPLVPPGPELTAQERTRYARHLLLPDLGSDGQRRLRAARVL
                     VVGAGGLGSPALLYLAAAGVGTIGIVDDDVVDLTNLQRQVVHGVADVGRPKTSSAAES
                     VRAISPATVVIEHTARLDESTALAVVADYDLVLDGADNFPTRYLVGDACARLGIPHVW
                     GSVFRFDGQVSVWWAGEGPCYACVFPSQPAPDAVPSCAVGGVLGAVCASVGSVMGTEA
                     VKLITGMGEPLVGRLLVHDALGQTWDALPVAADPGCRVCGSGADVARPLGWATGIRTE
                     GADAGASPPDRPTISVDRLREELDAGAVVVDVREAGEREIVALAEAVWVPIGDLRAGA
                     GVPGVDAGVRVYVHCKSGGRSAEAVDLLRARGVDAVNVEGGILAWVRDVEPHLPTY"
     assembly_gap    7061..7160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7269..7928
                     /locus_tag="PL014_00310"
     CDS             7269..7928
                     /locus_tag="PL014_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOT55560.1"
                     /translation="MRNGDRTQLLRVQNFMLSTDGFGTGEGLSLERPFGHADPAALAS
                     WAGATASWPNRTDPGGTRGLDDYFTRDFSTNIGAEIMGRGKFGPQQGPWEDLQWQGWW
                     GEEPPFRTPVFVLTHHVRPSITLSDTTFHFIDADPHEALRQAKEAADGKDVRLGGGVR
                     TVREFLEADLVDTMHVAVAPVEIGRGGRLWESPDELLDRFHLEVVPSPSGVVHHLFWR
                     R"
     gene            7974..11057
                     /locus_tag="PL014_00315"
     CDS             7974..11057
                     /locus_tag="PL014_00315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOT55561.1"
                     /translation="MLVLRRPDLAEAAAPVLDGSQQAAVEHRAAVVRVLGAPGTGKST
                     VAVERIAAAVAEGLTADQCLLLAPTRRAAARLRDAVTARLGGTTTTPLARSHQSFGFG
                     LLRQAAALAGDPTPRLLSGPEQDVILGELLAGHAAGTAPAPQWPAELHLALPTRTFRR
                     ELRDLLMRAVELGLEPEALAELGRDHGRGEWVAAAHVLREYDEVTALSAAGAFDPAWI
                     LGAAADLVEDDPTSLPQSLRLVVVDDAQELTPPGLRLLQETVTASAAQLVLIGDPDAA
                     TETFRGADPRLFLTAWPQADTITLGACHRSGAPLRAAASRVVGHIGVVGGVAHRAVDP
                     DPRDGRVETHLVRTASQEAGYIAGRLRTAHLIDGVPWSRMAVIVRGGSRTATLRRVLR
                     SSGVPVDTTTADLPVADEAAVRPFLTLLTWALEVIAGGEHLDPAQVVDTLTSPVGGAD
                     AVSLRRLRRSLRRLELDGGGGRPSDVLLAEAVLAPGALDHVGPEGQPARRVHRVLATA
                     VDALREGDGVEVVLWRMWSASGLAQPWRDAALTGGSAGGRADRDLDAVLGLFDAAARF
                     VDRLPGASPADFLDHVLAQDVAGDSLVARAPAGESVALLTPAASAGREWDLVVVAGVQ
                     EGVWPDLRLRGSLLGSTDLVDVLAGRGDDPRAARAQVRHDETRLFHVAVTRAREHLVV
                     TAVRGEDEQPSPFLDVIDPLPQARTFTDVPRPLTLAGLVGELRREVAGEDPARRGAAV
                     TTLARLASHHVPGADPAQWWALRDVSDTRPRRAPDAPVVISPSAIDPFIRCRLQWVLR
                     AAGGDGPSMGAQDIGTLVHDVAHELGDTDAATYAAEVEARWGRLGLAPGWLSRRSLAQ
                     ATAMTDRLARYVTESDAAGWRRAASEARIEAAIGRARITGRVDRVEVSPTGQVRIVDL
                     KTGASKPSVAEVSRHGQLGAYQLAVQHGALPDVGTEPGGAALLHLGKAANLTTTVQVQ
                     QPLDADDDPQWAHDLVEQVAQEMSGAVFSATPGTKQCGTCQVKDSCPALAEGRRL"
     gene            11054..14350
                     /locus_tag="PL014_00320"
     CDS             11054..14350
                     /locus_tag="PL014_00320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOT55562.1"
                     /translation="MTVPRWSAVALAQALGQHAPTPEQAAVIEAPLRPLLVVAGAGSG
                     KTETMAARVVWLVANDLVRPDEVLGLTFTRKAAGELSERLSARLATLREAGIWVPQEE
                     DGAAVLDDAPTVSTYHAYAGRIVREHGVRLGVEAESRLLSEAAAWQVAHESVVAYDGP
                     MDEVEKAESTVTTAVVDLAGEMAEHLVDVDAVAAHLDEVVAALELIPRGEASTKKAFP
                     KEVRDAIAVLRERRAILPLIKDFQALKRSRDSMDFADQVQLAARIAMTVKQVGVGERS
                     RFRAVLLDEFQDTSEAQLQLLRSLFVAPGAAVPVTAVGDPHQSIYGWRGASSTTLHRF
                     RADFVDDAGPTEVLPLSTSWRNDRAVLEVANHVAQPLTRATSVPVHRLGARTDAGPGH
                     VATARLSTLEDEAAHVADWVRARLRRPGRATAAVLCRKRSQFDPVIEALEERGIPYEV
                     VGLGGLLHTPEVADLVALLWVVQDPSRGDRLMRLLTGPSCRLGAADLDGLGAWARERQ
                     RTGRDRRRTDLSRDASEQASIVEALDDLPPATWVGPEGQSLSPTALARLKGLADTVRH
                     LRTLTGLGLAELAAEAEVALGLDIEVLARTTWSPGAARAHLDAFADVAATFAASADRS
                     TLGGFLAWLDAAVDEERGLDLGWVEARPDAVQVMTVHAAKGLEWDVVAVPGLVESSFP
                     AHSGTSTTVKDGAWNHSTPTDKGWLAGLATLPYDLRGDADGLPHFSWRGPHTWDTIAG
                     TSGEYHRFRGAVAAHGILEERRLAYVAFTRARHDLLLTSHVWGTAGTPRLTSRFLLEV
                     RGSGLAQASGPWVDLPPIDPKPQNPRTAEQVDVAWPTQDHVPRRQGLLEPARAIAGAV
                     ATLSQAPDGSGVAAPRGPWDEEVTMLLAERAARGRGADVDVVLPAHLSTSALVALAED
                     SARFTLDLRRPMPTPPALSARRGTAFHAWVEEHYARAAFVDIDELPGSADDGAVDDDL
                     AALRASFSASEWADRTPAEVETSVETVLDGIAVRGRIDAVFEEVGDDGQPSWVVVDWK
                     TGAAPSGSRASARTLQLAAYRIAWARVRGVPVERVRGAFFHAATGETTWPDLPGVDGI
                     TAVLRAARPG"
     gene            complement(14316..15641)
                     /locus_tag="PL014_00325"
     CDS             complement(14316..15641)
                     /locus_tag="PL014_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT55563.1"
                     /translation="MDRSPAYLAALASAAVSGLDPVSVEALSSTPDQSFDVAFIQDTE
                     HRRWVVRVPRSDVAGAEMDRTVALLALLGRRLPFAVPAPKGFVALAEGGRAAVYPYLP
                     GHNLDFAELPAGPGLAAELGRAIAALHNTDPAIYDEAGLPSYDADAYRSRRLAELDRA
                     AETGRVPTTLLTRWEKALEDVTLWRFAPTATHGDLTGDQVLAVFTDDSDASTGTIRAM
                     TGWEDAKVADPADDFAPLVADASPEAVETVLEAYAHARVERPDTNLIVRARLVAELGL
                     LGTLMTALSRQDAGAVEVLTTQLRRLDDAVHAGEEPDDYRRTSLAPIGIRTRPTPPPA
                     LVVDEDDEELPELVDPDLMETEPVDSELVETDRDDTVAVPVAVPVTVPVAAGDEVPAW
                     LELEHSEDDEPDEDHAPGQDAALAETDEEDADELGSGEVSPDERPSAPQ"
     gene            15683..16636
                     /gene="nudC"
                     /locus_tag="PL014_00330"
     CDS             15683..16636
                     /gene="nudC"
                     /locus_tag="PL014_00330"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOT55564.1"
                     /translation="MPDRAAILPDLALSRSVLDRRGDLRADPSLLTRLLADGDTRVLR
                     LVGDRAAVIAGGSTGDGSDEVTRVVFDAPTPEDLDRLGLYLGDAAGVSYVGVVADDLT
                     ASVEGDDAWRTLRQVGATLADLDAHALVTTLALANWHRSHTHCPRCGTPTEPVLSGWI
                     RRCPADSSDHYPRTDMAVIMAVVDADDRLLLARGKGFRGNGMSVLAGFVEPGESLAAA
                     VAREVLEEVGVAVTDVTYLGDQPWPFPSSLMLGFTARALDTDLHLQAEEIEAARWFSR
                     DDLVAALADGSVVISGQLSISRRIIEHWFGGPIDSPDIPLR"
     gene            complement(16670..16939)
                     /locus_tag="PL014_00335"
     CDS             complement(16670..16939)
                     /locus_tag="PL014_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097573 - glutathione oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HOT55565.1"
                     /translation="MSDAAVTHLPAPGTVTMFTTTWCGYCRRLKSQMEREGISFTEVD
                     IEHEPGTAEFVMSVNGGNQTVPTLLFADGSAATNPTIAEVRSRLT"
     gene            17078..19213
                     /locus_tag="PL014_00340"
     CDS             17078..19213
                     /locus_tag="PL014_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HOT55566.1"
                     /translation="MSSLPVHPGADAVLDALDPEQREVAAHPSGPMCVLAGAGTGKTR
                     AITHRIAYGVLSGVYQPQRVLAVTFTARAAGEMRTRLRDLGVGGVQARTFHAASLRQL
                     HYFWPQAIGGASPEVMPHKASAVAEAGSRLRLRLDSPAIRDLSSEIEWSKVSLLTPES
                     YAAAAAAARREPPAGLDATAMARLIAAYEEVKTDRGVIDFEDVLLLMVGILREHEEVA
                     RAVRNQYRHFVVDEYQDVNRLQQTLLDLWLGERRDICVVGDPAQTIYSFTGASPEHLL
                     SFRTRHPDARTVELVRNYRSTPQVVTLANLVLRGPGGQRRSGSVELRAQRADGPVPEL
                     VVADNDPDEAAAVATRIASLLDAGHPPSEVAVLFRTNGQSEAFESALAERGIPYLVRG
                     GERFFSRKEVRDAVVLMRGAARSDDGSVPLPDLARDVLLGAGWSREAPTSGGAARERW
                     ESLAALAGLADDLFATDPQARMPALVRELEERAAASHAPTVQGVTLASFHAAKGLEWD
                     TVFLVGCSDGLLPITMADTPEAVEEERRLLYVGVTRARERLVLSYAGARTPGAKATRR
                     PSRFLDGTSSVLGDGARSQARRRKAPGTGGGAGGGKGKPAVRSTCRTCGADLGTARER
                     TIGRCSTCPPTMDERLFEALREWRLETAREADVPAFVVFTDATLTAIAEKVPRDVAAL
                     SRITGVGPAKLERYGAAVLSLLEEFSEAQ"
     gene            19383..19610
                     /locus_tag="PL014_00345"
     CDS             19383..19610
                     /locus_tag="PL014_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55567.1"
                     /translation="MTKDLAMSAVREICARPWRSANTAHAGTFGSDQGRTVSYPGSAT
                     RDDKLVAGVFAVLGNALAQPFGYPRLEAPPV"
     gene            19866..20153
                     /locus_tag="PL014_00350"
     CDS             19866..20153
                     /locus_tag="PL014_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT55568.1"
                     /translation="MMLTQLLDLHEASDALGEAIPCRENDAELWFADTPDGVEFAKAL
                     CGTCPARDVCLSGALDRREPWGVWGGELFVQGAIVARKRPRGRPRKTDVAA"
     gene            20189..20320
                     /locus_tag="PL014_00355"
     CDS             20189..20320
                     /locus_tag="PL014_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55569.1"
                     /translation="MYFRRMQRIRRTAHQRRVHEAQRQVQAAAEREQLRAVIHSHVR"
     gene            20481..21053
                     /locus_tag="PL014_00360"
     CDS             20481..21053
                     /locus_tag="PL014_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HOT55570.1"
                     /translation="MASLTIAQDPSADRVLSQDPFALLTGMLLDQQFPMDRAFAGPAK
                     VLERFGTLDPAAIAAADPEAFADLCATPPAVHRYGRSMAGRIQALAVVVRDEYDGDAS
                     AIWSTATTGNELFARLKALPGFGEQKAKIFTALLGKQLAVRPDGWQEVCGAYAEPGSF
                     RSVADVVDAESLQKVRDFKKAAKAAAKAGA"
     gene            21059..21286
                     /locus_tag="PL014_00365"
     CDS             21059..21286
                     /locus_tag="PL014_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55571.1"
                     /translation="MAEHDDTAPTADAGRPASDIVCASCGATPDGAEALSLARITWAR
                     GVENGRDTWTCEQCSRRFLRSIEGKLDSSWW"
     gene            complement(21298..22635)
                     /locus_tag="PL014_00370"
     CDS             complement(21298..22635)
                     /locus_tag="PL014_00370"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOT55572.1"
                     /translation="MTDIPRSSIARTAKLVGLPLGHAGRVAMGVGKRVGGRPAEAVAA
                     ELQARTAEQLFTVLGQLKGGAMKFGQALSVMEAAMPEELAGPYRATLTKLQESGPPMG
                     ADRVHEILARELGPRWASTKLVEFDDVPAAAASIGQVHKGVWRDGREVAVKLQYPGAA
                     EALLSDLNQISRVARMAGSWVPGIDIKPIMAELKARMSEELDYHLEARNQRHFANAFR
                     GDEDVLIPEVLVDSKHVIVTEWIDGTPLASVITNGTTQERDEAATLYLEFLLRAPNRA
                     RMLHADPHPGNFRITDDGRLAVFDFGAVNRLPHGLPASLGRVLTEALAGDAVALEAVL
                     REEGFIRKGIAIDPDALLEYLKPLVEPLLHDQFTPTREWLRGAAATLQDPRRPQFLIG
                     LKLNLPPEYLLIHRVWLGGIGVLSQIGGTVPLRELVCAHLSGIDESRLPPSPS"
     gene            complement(22632..23243)
                     /locus_tag="PL014_00375"
     CDS             complement(22632..23243)
                     /locus_tag="PL014_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55573.1"
                     /translation="MQGRSGAGHVVVDGTGLFADELVAQLRRCEVPVVGGAHAADGAE
                     LALAEGEPAPTAVVVVARDRPPRWAGTPWQARGIPHLPVVVGAGRVVVGPLVLPGSTA
                     CLRCCGDAWAEARGGASPRTRAAAGAPGADGIRPDHPASDTPSGTVVLACAVATVIVL
                     STLRGDVSLGGISTEIGLADVTLTHRLWNVRADCGCARVRMAG"
     gene            complement(23475..23636)
                     /locus_tag="PL014_00380"
     CDS             complement(23475..23636)
                     /locus_tag="PL014_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HOT55574.1"
                     /translation="MADETYKGEFYCVKCKEKREAEGKVVVSENGRRMAKGVCPVCGT
                     NLNRILGKA"
     gene            23850..24458
                     /locus_tag="PL014_00385"
     CDS             23850..24458
                     /locus_tag="PL014_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOT55575.1"
                     /translation="MSREDVEVRRSRRRRRTVSAYREGERTVVLIPASFSAAEEERWV
                     TRMLTRLTAGDRRRARGDDELQARAAHLSQKYLGGHARPTSVRWVSTMGRRWASCTPG
                     DGTIRVSDRLRDVPNHVLDYVLLHELAHLLVPGHGPAFWRLLASYPKLDRARGFLDGF
                     AHAEGLAPGPDEDPVDGPADVESAVEQSAVEQSAGVEGTQRE"
     gene            complement(24426..24992)
                     /locus_tag="PL014_00390"
     CDS             complement(24426..24992)
                     /locus_tag="PL014_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT55576.1"
                     /translation="MSEGGFAHLRADAVDVLSRWTAPDAGQARLREEYLAHLAAHPDG
                     AAKTGPPAHLTASCLVVDAARAQVLLTHHRKARAWFQFGGHLEAGDASVWAAARREAR
                     EESGIGALEPLPDPVQLDRHELVGAFGHCREHLDLRYVALAPDGAAGVVSEESLDVRW
                     WPIDALPAGTRAELAPLVSLALRALDAG"
     gene            complement(24989..26380)
                     /locus_tag="PL014_00395"
     CDS             complement(24989..26380)
                     /locus_tag="PL014_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOT55577.1"
                     /translation="MNDPADDASALPPEIEKLLRDLTGGAPLDPQLASMMQGMGLDKV
                     DPQMLAMVMGQVQAMFSAPESEHAVNATLATDMARKTVSAQGDTLATSADQREAAEAV
                     QVAGLWLDAVTDLTTPGLHGVAWSAAEWVEATMPVWTDLVEPIAAGVTDAVTTALGSQ
                     LEQLGNLENRGDAGIPGLPGGIPAGMLDQAGPMLRRMHGSMFSMQLGQGTGTLAGEVL
                     TGCEVSLPLVPAPTVALMPSALRTFAQGLEVDEPQARLYLAVREVARVRLFGAVPWLA
                     PALLAAVRDYAADITIDTSAIEEAVRSIDPSDPEAVQQALQGRMFAPQPSPAQQAALT
                     RLETWLALVEGWVDVVTDRATREHLPQADALGEAVRRRRATSGPAEKAFEALVGLELR
                     PRRLRDAANLFSALEDSGDAATRDAPWRHPDLAPSAADLDDPLGYVERTSSPTTDDMD
                     AALDALLRGEDPA"
     gene            26483..26917
                     /locus_tag="PL014_00400"
     CDS             26483..26917
                     /locus_tag="PL014_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010533821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOT55578.1"
                     /translation="MTTDPDPVALVALRDQPLSVDEVLDAVRHPSCGGIALFVGVVRD
                     HDHGAAVTSLDYEAHPSALDTLNRVCAEVLGRSQARRVAAVHRTGSLAIGDLAVVVAA
                     SAPHRGQAFDACRDLIDTLKAQTPIWKHQSLQDGSTEWVGLP"
     gene            26938..27984
                     /locus_tag="PL014_00405"
     CDS             26938..27984
                     /locus_tag="PL014_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOT55579.1"
                     /translation="MRPGRISAALLVGSFLVIAVAAALTFVGLPYVVMSPGPATNILG
                     ENDGKPLLTVDGAQTYPTSGSLDFTTVSVTGGPGVKVTVFDVVGARLSGSDEVLPVEQ
                     VFPPEASQEQVEQEGAAEMANSQSVATATALRALGRDVEQTVTIGEVLEDGPSTGVFE
                     AGDVLVSVDGDPATSSDAVRAAVQRHEPGETFPVVIERGGSERTVTARTAEAGGRTVI
                     GVGLRMDYDLPIDVTLNTGRVGGPSAGLMFSLAIYDVLTPGELTGGKNIAGTGTMQDD
                     GSVGPIGGIRQKLVGAKRAGASYFLAPADNCDEVVGHVPDGLEVLKVATFDDGLAAAN
                     GIAKGSVEGLPTCG"
     gene            complement(28015..28572)
                     /locus_tag="PL014_00410"
     CDS             complement(28015..28572)
                     /locus_tag="PL014_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPA1309 family protein"
                     /protein_id="HOT55580.1"
                     /translation="MNSPTDPAPAAPQPVTDPLTLAALDTERHVARSGWDQPVRLFAL
                     VPTADLLAHEPQLAQGLASHDAVAGALSAVEQEDLPRSDGLEDLLAQIAWPEQVLGCA
                     LAVERIVVPPEAERDLPSDDAAALQAIAEHPDRADVRLLVAVTRDGAARCLLRQRAHD
                     TDDRVALGEDIAPGLVHALRATLTP"
     gene            28822..31728
                     /locus_tag="PL014_00415"
     CDS             28822..31728
                     /locus_tag="PL014_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOT55581.1"
                     /translation="MLAVLALVGGIAADVITDVWWYDSVGFRGVFVKEIAVKAGLFIG
                     AGLLTGAAVAISLVAAYRTRPLYIPMTQAQQVLEQYRQAIEPLRRLAVWSIPIILGVL
                     AGSGAMGAWRTLLLWMNREPFGIQDPQFGMDVGFFVFTLPWLRFVVSFITVVLVLAFV
                     AGAFTHYVYGGLQLPGRGPTTRAAYVHLGILGALIALTRAASYWLDRYTLATQKGSLL
                     TGITYTDDNAVLPTKAILAVAALMCAVFFLAAIWSRSWRLPLTGIALLAVTAVVAGGV
                     YPALIQSLRVKPSEKSLEAPYLERNITATRTAFGLDRVKRIQNPAPEATEDKDVLRTA
                     ANDIPGVRIIDPNVVAPTFRQLEGQRDYYAFPDTLDVDRYTINDQTRDAVVAVREINL
                     DGLPDNQRNWLNDHTVYTHGYGFYAAYGNQRTAEGDPVFFEGGGRTSLGEYEPRIYFG
                     ELSPTYSIVGAPEGDSPREFDYPAGGDGDVAVNNTYEGAGGVEVGSLVRRLAYAVKYR
                     EANFLLSDAVNSESRILDHRTPTERVERVAPWLRLDGNVYPAVVEGRIQWIVDGFTTS
                     ASYPNSRLIELADATSDAVTQRSSVVTVGAGQVNYVRNAVKATVDAHDGSVKLYGWDT
                     KDPMLKAWSKAFPGSVRPLSEISGDLMSHIRYPQDLVKVQRELLNEYHVTNPGDFYSN
                     SDKWRVPRDPAQTAQDQPVVFQSIAMPGEDEAAFSITTPFVPASNNEAGREILRGLLA
                     VDADAGSEAGKPAEDYGSLRLLEYGSATPAGPGQVLNQIQNSPVRSQNAAEQLSLAQY
                     ITNNSGGGKTLTFGNLLTFPLEGRMFYVQPIYVQAAAGSGSFPQNKITVAVYGTTVAW
                     GDTLEQAVSGLFGQGDGSETPTTPTDPGTGTPTPPDTGQPTGSAAAQLTAALAEMQAA
                     YTAGQEALKSGDFTAYDQAQKRLDAALKKAAALAPQLNPDGAGATPSPSPSPTG"
     gene            31854..31927
                     /locus_tag="PL014_00420"
     tRNA            31854..31927
                     /locus_tag="PL014_00420"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31888..31890,aa:Met,seq:cat)
     gene            complement(32087..34882)
                     /locus_tag="PL014_00425"
     CDS             complement(32087..34882)
                     /locus_tag="PL014_00425"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010143048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOT55582.1"
                     /translation="MPPLPRDASTLDPGAVLAELGTDPVGGLTTREAQRRLAQVGPNE
                     LEATAPVPTWRKVLEQFRDPLVYLLFAAIVVSLVAWAVEGADGVPVDALVIAVIVVLN
                     AVLGYVQQARAEDAVAALRRLGATSATVVRDGDRVTVPTRELVPGDLVVLAEGDTVAA
                     DARLLSAAALTVSEASLTGESEPVLKDPATLAAPAPLGDRLDMVFNGTAVTQGVGRAV
                     VTATGMSTEMGRIAHLLHDTRENPTPLQVEIARVGRTLGIAVVVIAVVVVATIVVFLG
                     VETPQDIVTTLLLGVSLAVAAVPEGLPAVLSVVLALGVQRMATHQAVVRRLSSVETLG
                     SASVICTDKTGTLTRGEMTIGRVVTAAGEVVVTGAGYRPEGTLEHDGIPLEHGSAAWR
                     QTALVVGGGSLTNDAVLTQEADGGFVVQGDPTEAAFLVAEAKLGTRALREGRFARVGE
                     IPFTSQRRLMSSLATDAERAGRIAVVTKGAPDVLLERCTHVQVGDDVEPLDDGWRTGI
                     LADVERLSGEAFRTLAVAYRPLDVTDPPEAVESLEEGLVYTGMVGIIDPPRDEAATAI
                     REARRAGIRVVMITGDHPRTAARIARDLGIGPHEPVAVTGAELEALDDDGLRETVRHR
                     SVYARVAPQHKLRIVAALQGDGKVVAMTGDGVNDAPALKAADIGIAMGRTGTEVTKEA
                     ADMVLADDDFATIVRAVREGRGIFLNIKKFLRYLLSSNMGEVLTVFLGVVLGGLIGLS
                     HGSDGLVLPLLATQILWINLLTDSAPALAMGVDDETEDVMGHPPRPLTERVIDRRMWR
                     GVVGLGLVMAAAALITLDLHLPGGLIEGDESIDTARTAAFTVLVLAQLFNALNARSES
                     VSAFRHLFVNRWLWLALATSVLLQVAVVHLPVLNEAFSTVPLTGQQWLVCVAMASTVL
                     WAAEARKLIARFSASRR"
     gene            34986..36383
                     /locus_tag="PL014_00430"
     CDS             34986..36383
                     /locus_tag="PL014_00430"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT55583.1"
                     /translation="MSTPHRVVVIGSGFGGLFGTQALKRRDDVEVTLIAQTTHHLFQP
                     LLYQVATGILSEGEIAPSTREVLARQKNARVVLGTVTSIDVHDRTVTSHVLDRELVTP
                     YDTLLVAAGAGQSYFGNDHFARHAPGMKSIDDALELRGRIFGAFELAELAETPAEAER
                     AMTFVVVGAGPTGVEMAGQIAELAHRTLRKEFRRIDPRNSRIILVDAAPTVLPSFGER
                     LGGAAKRRLEEMGVDVRPGTMVTDVDAAGITVREADGSSTRIEALTKVWAAGVQASSL
                     GQQLALQTGAELDRAGRIKVLPDLTLPGHPEVFVVGDMISLDNLPGVAQVAIQGSRFA
                     AKQIKRRLDGKEPEPAFQYFDKGSMATISRFSAVASIGKFRFSGFFAWLLWLAIHLVY
                     IIGFKHRVTTLLHWTVSFLGRGRAERVATEQQVFARRAMDAMSQDEQPSIPTNPADTQ
                     VAGVAEIEEQHRASA"
     gene            36471..37820
                     /locus_tag="PL014_00435"
     CDS             36471..37820
                     /locus_tag="PL014_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT55584.1"
                     /translation="MPLDYPIHRRTLPNGLRVVVSPDHTVPNVTVNLWVGVGSRHESP
                     GRTGFAHLFEHLMFQGSKNVPSGEHFEALMAQGGRLNATTWFDRTNYFETVPKGAVEL
                     ALWLEADRHGHLLDAVTQENLDNQRDVVKEEKRQRYDNQPYGNALIDVYAATFPQGHP
                     YHHPTIGSMEDLDAASLADVHAFFRAHYGPDNTVLTLCGDITPEHGFELVERHFGPLP
                     ASAEPRRENHPQLEPLAQPVRVERRESVPNDRLHIAFRLPVDETDEFLAAAVALDAIG
                     GLSTSRLVRRLVRRDQIALGAHATAWGFVDGASLGFVVLDVAPGQDPDAVEAAFVEEL
                     TAFLAGGPTAVELEASLAQSERSWLSALASQEERADAISHHLLLHDDPEVVNTHLDRL
                     QDVTAEQVLAAARRWLQPQSRAVVAYLSAPADDEAVGDVAEDSAGDSDAGANEEEVA"
     gene            37817..39166
                     /locus_tag="PL014_00440"
     CDS             37817..39166
                     /locus_tag="PL014_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT55585.1"
                     /translation="MSTARPQVTPPQPWAFPVPTEHVLGNGVRVLLHDLPGQYVLSLR
                     VVVPLAVSAEPAGQEGIASMTARLLDEGTASLGSDEFAELMERHGMVLGAGVSDGGLL
                     VDVDVSQRHLATAAGLLTQALAAPAFPEDEVRRILRNRLAEIEQERASSAHRASKEFA
                     RTLWDPADRASRPTGGTPESIGALTRDDLVEFHATHVGPAGATVVLAGDLTDVDALAV
                     LEATLGTWSAPRHTPSARPVPPLPAADAARVVVVDRPGSVQTEIVVGRPGPDRSTPHG
                     WAPHPVLSFVLGGSPSARIDAVLREEKGYTYGIRSTFRPRVAGGTFVTSGSVRSEVTG
                     ESVEILLGILDGARDGFTDAELRTGVDYVAMTAPGRYATADAVADESATLALEGLPGD
                     FTTRNLLAVNALTLADLDAAYGRVVTGEWTVVLVGDAAQVVPQLDGRVPGSVSTVAL"
     gene            39156..39710
                     /locus_tag="PL014_00445"
     CDS             39156..39710
                     /locus_tag="PL014_00445"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOT55586.1"
                     /translation="MPSDVVPGAVRGPSSSDAGASRSVVMASPIGRLELIEAHGALTG
                     IRFDAPADGTAGHERGGSPVLTAAHRQLSEYFEGRRREFDLPLRPAGTEFQRRVWDVL
                     ATLPWGATTTYGEIANRLGLPPGASRAVGAANGANPIPIVLPCHRVIGSDGTLTGYAG
                     GVERKAMLLRLEGVASEADQASLF"
     gene            complement(39778..40563)
                     /gene="hisN"
                     /locus_tag="PL014_00450"
     CDS             complement(39778..40563)
                     /gene="hisN"
                     /locus_tag="PL014_00450"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOT55587.1"
                     /translation="MRAYDDDLRLAHVLADAVERVTTARFRAEDLRVEAKPDLTLVSD
                     ADRDAEELIRGQLARTRPRDAVIGEEFDPTGNSARQWIVDPIDGTHNYVRGVPVWATL
                     IALVDDGVPVIGLVAAPALGRRWWAALGSGAWAGKSLTSAKRIHVSSVSRIEDASISC
                     SSWTSWADVDREREVLDLARECWRERAFGDFWSYMLLAEGCVDIAAEPELGLHDMAAL
                     VPIVTEAGGRFTSLAGVDGPFGGNAVATNGILHDEVLRRIGTP"
     gene            40846..42465
                     /locus_tag="PL014_00455"
     CDS             40846..42465
                     /locus_tag="PL014_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT55588.1"
                     /translation="MSAVALLAAGCGSGDSEPEATTEAKTGGEITVRGCNPENPLVPS
                     NTTETCGGNVIDAVFAKLVHYDAETAKPEMDVAESIETTDNQNFTIKLKSGWKFHDGT
                     DIKAKNFVDAWNYAAYGPNAQSSGYFMEPIEGYGDLQCAGTANPDDPCEGFTPKAKEM
                     SGLKVVDDLTFTVKTTLKVANFPVRVGYSAFSPLPDAFFADPEGFGDKPIGSGPYKLD
                     SWTKEQSIELSKFADYAGDFGGKVDKITFKIYADPDAAYNDVIASNLDITDEIPASAL
                     IDDKYKSDLPDRNAQKETGVIQTVTVAPVKVDPNYADPKIRQAISMAIDRETIIEKIF
                     NNTRVPATGWVSPVVDGYKAGQCGEYCTFDPEKAKAKLAEAGGFKGDKITLSYNADAA
                     HKEWTEATCNSIKQTLGVECVATGVVDFATFRTQIGERKMKGMFRTGWQMDYPSIENF
                     LAPIFATGSSSNDGDYSNEKFDTLLDEAAAETDAAASNAKYQEAEALLAADMPSIPMW
                     YGKTTMGWSEKVTGVKITAFGTIDFSSVSMK"
     gene            42681..43610
                     /locus_tag="PL014_00460"
     CDS             42681..43610
                     /locus_tag="PL014_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55589.1"
                     /translation="MGTYIIRRLLQMIPVILGATFLIFTMVFALPGDPLAGKCGERPC
                     PPAYAAEFRERYNLNDPLPVQYAKYVSNLVQGDLGETTNGIPVLDQLKTRYPVTAKLA
                     LMAIAFEAIIGIFVGVLTGIRRGGFLDNLVLISTLVVISIPVFVIGGVSQYFLGVKLG
                     WFPVTVTSSNPSFYELMLPAMVLGSLSLAYVSRLMRTSLVENLRADYLRTAVAKGLTK
                     RRAIGLHALRNSMIPVITFIGFDFGALLGGAIVTEAIFNIPGIGGYLFRSIRARDGAS
                     VVGTVTVLVIVYLFVNLLVDLLYGLLDPRISHD"
     gene            43603..44526
                     /locus_tag="PL014_00465"
     CDS             43603..44526
                     /locus_tag="PL014_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55590.1"
                     /translation="MTEAAAVVSETVAPNGSSDENRSLASDAWRSLRKNPIFWASAVL
                     ISIFVLMAIFPSLFTSTDPREAVLSESRSRPGDGTWFGRDIQGYDIYARCIYGARASI
                     LVGVLTTILTVSIGGFIGVMSGYLGGAADAVMSRVGDVFFAIPLLLGAIIFLVSLPEF
                     FSSNAFLGALKVSLALAVLGWPSMARLMRSSVIQVKPNDYVQAARALGASPSRIVRSH
                     ILPNSVAPLIVVATISLGGYITAEATLSFLGIGLQAPAVSWGIDISAALVGLRTTPHM
                     LFFPSLFLSLAVLGFILLGDAVRDALDPKNR"
     gene            44542..45573
                     /locus_tag="PL014_00470"
     CDS             44542..45573
                     /locus_tag="PL014_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55591.1"
                     /translation="MTTIQDTQTAGSEYQPVDGLLLEVEDLFVEFHTPDGVATAINGV
                     SFELRQGESLAILGESGSGKSVTAQAIMGILDMPPAVIPSGHIRYCGQDLLTMPEEQR
                     RKTRGPEISMIFQDALSSLNPVFPVGWQIAEMFRVHRGLNKSDALEKAIGLMERVSIP
                     AARERVKAYPHQFSGGMRQRIMIAMAIALDPAVLIADEPTTALDVTVQAQIMALLQEL
                     QEERHMGLILITHDLGVVADVADRIAVMYTGRLVETAEVHELYARPAHPYTKGLLDSI
                     PRLDQKGQTLAAIGGLPPNLLRIPPGCPFNPRCHMARDVCRTDLPPLHEVTPGRFSAC
                     HYAEEVINA"
     gene            45566..46774
                     /locus_tag="PL014_00475"
     CDS             45566..46774
                     /locus_tag="PL014_00475"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOT55592.1"
                     /translation="MPEVILSAQDLVKHYPIKKGILRRTVGHVKAVDGVSFDLHQGET
                     LGIVGESGCGKSTLGRLLMRLEEPTSGKVIFGGDDWSGASGRDLRRMRRDIQIVFQDP
                     YTSLNPRMTVGDIVGEPFEIHSDVVPKGGRRKRVQELLDLVGLNPEHINRYPHQFSGG
                     QRQRIGIARGIALNPKVLVCDEPVSALDVSVQAQVVNLMEKLQNELGLAYVFIAHDLS
                     VVRHISDRVGVMYLGRMAELGEEDEVYTRPTHPYTQALLSAVPVPDPTLRGKREQIVL
                     VGDVPSPANPPSGCRFHTRCWKAQDRCKTEEPQLEQRPDGVGAHRSACHFAEPRVIVE
                     TVDVSGVVPDERFASTDVRDTVVSDDAPADLQRMIDRSEASSGDQAYAPQDVTRLIDE
                     QRGDHRPPTV"
     gene            complement(46833..47858)
                     /gene="rsgA"
                     /locus_tag="PL014_00480"
     CDS             complement(46833..47858)
                     /gene="rsgA"
                     /locus_tag="PL014_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT55593.1"
                     /translation="MARSSRDYDESDVRIRPNRRGSRPRSKDRPAHEDAVPGQVITVD
                     RGRYTTLVGDGGDRHVVMAMRARELGRKGIVVGDRVDVVGDTSGADGSLARIVRVQPR
                     ETVLRRTADDTDPVERVLVANADQLVVVSALANPEPRPRLIDRCLVAAYDAGMEPLLV
                     LTKADLTDPGPFLEQYAPLSVRHVVTARRDTRTDGLDTLREALSGRVSVLVGHSGVGK
                     STLVNALVPQALRATGVVNDVTGRGRHTSSSAVALALPDDDGWVIDTPGVRSFGLAHV
                     DLSRIVQHFADLEPGTATCPRGCTHDEPECGLDAWVASGDAGPAGAARLDSLRRLLRA
                     RSAPTTD"
     gene            complement(47858..49150)
                     /gene="aroA"
                     /locus_tag="PL014_00485"
     CDS             complement(47858..49150)
                     /gene="aroA"
                     /locus_tag="PL014_00485"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT55594.1"
                     /translation="MPSADWAAPSAHGPVDAHVVLPGSKSLTNRYLVLAALANDTSRL
                     RAPLRSRDTLLMAGALRTLGAEIEDVPGGDWIVSPGPVRGGGQVDCGLAGNVMRFVPP
                     LAGLASGPVRFDGDPHARQRPMAPVIGALRALGVDLDDGGSGSLPFTVNGRGRVPGGS
                     VTLDASASSQFVSGLLLSAARFDEGVTVHHDGGAVPSLPHIEMTVETLRDAGVVVDDG
                     EVHTWRVEPSEINALDVQVEPDLSNAAQFLAAALVTGGRVHVPGWPQYTTQGGDFLRD
                     VLDMMGAEVVLDRGGLTVTAGDGIAGIDVDLHDSSELTPVVAALCALAEAPSVIRGVA
                     HIRGHETDRLAALRTELTALGAHVVETEDGLKITPAPLRAGLFHTYADHRMVMAGAVL
                     GLVVPGVVVEDVGTVAKTMPEFTTLWERMLSERATAER"
     gene            complement(49137..49679)
                     /locus_tag="PL014_00490"
     CDS             complement(49137..49679)
                     /locus_tag="PL014_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOT55595.1"
                     /translation="MTLVRRVARPLLAAIFIAGGLDQFKHPTAKAKTAGPLLAKVGPR
                     VGLPDDPELLIRANGMAMVGAGALLATGHLPRVASSVLAVTLVPTTVAAHAFWKEQDP
                     EVRERQKVQFLKNLGLLGGLLLAAVDTEGKPGLAYRAHLASDSAHRAVRTTRREARHA
                     ARAAARETKLKAVQAQHALS"
     gene            49762..50601
                     /locus_tag="PL014_00495"
     CDS             49762..50601
                     /locus_tag="PL014_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOT55596.1"
                     /translation="MCGRYAATANPDELVLEFEVDDDRTGEPTRSILVSPQEPPPGRP
                     DHNVAPTKQAPVVLERPPRDDRDAPAVRQLRLLTWGLVPSWAKDTRGGARMINARVES
                     VAEKPSFAKALATRRALVPARGWYEWQVSPTALDAKGKPRKQPFFTSRVDGASVAMAG
                     LYEFWRDPSVADPDDPMAWLTTFTVITGPAEPGLDRIHDRAPIVLEPRHWDAWLDPAT
                     GAAEVLGLLEPYPPGRFTAWPVSPAVSSNRANGPQLLDPLPEDQLVGVVDPVTGEVIG
                     APR"
     gene            50598..51230
                     /locus_tag="PL014_00500"
     CDS             50598..51230
                     /locus_tag="PL014_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55597.1"
                     /translation="MTRSETTALVETPEGPARTTVTQPDGIPVGLLVLGHGAGGLRWT
                     DDVTASRDAAVDAGWTVALVDQPWRVAGRRIGGQPASLDRAWLAVLPTLHAHRPNGGP
                     LVVGGRSAGARVACRTAAAVGANAVLALSFPLHPPGRPERSRGEELREPGRHGITVHV
                     IQGLSDAFGTPEEVREVLTGAAVHEVAGTHSLERSAALVATAVTSALSRC"
     gene            51377..>51637
                     /locus_tag="PL014_00505"
     CDS             51377..>51637
                     /locus_tag="PL014_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOT55598.1"
                     /translation="MTTPTTPVDESPAEPIDVATESTADRQARFEREALPLLDQLYAA
                     AMRTTRNPADAEDLLQETYAKAYAAFHQYQPGTNLKAWMYRIL"
BASE COUNT         7217 a        18608 c        18289 g         7423 t
ORIGIN      
        1 atcgccgaga cccaggagat gctcgacttc tgcgccgagc acggcatcgc cgcgacgatc
       61 gaggtcgtgt cagcggccga tgtcaacgag gtctgggacc gggtggtcga cggtgacgtg
      121 cgctaccggg ccgtcatcga cacgagcacc atcaccccgg cctgacggtc ctgctgctgc
      181 gggactgacc acgcagactg cacgacatcg gggtgatcac cccgatttcg tgcagtccac
      241 ccgtcgtcag tccagccgaa cgcgctcgcc gacgcgcggg accaccgctg tccagcccag
      301 ctcgcgcgcg atgcggtcgg cgagggcccg tgaggccgcc ggctcaccgt gcacggcgta
      361 cagcgtcgtg ggtggcgtct cgatccggct cagccactcg atgatctcgt cggagtcggc
      421 gtgcaccgag aattccgggt cgtccacgac ctcggcgcgc accggcacgt agcggccgtg
      481 catcttgacc tcccgggcgc cctcgagcag gtctcgtcct cgcgtcccca cggcctggta
      541 accggtgagg acgacgcagt tgcgggggtc gccgagctgg tgcgcgaggt ggtgcacgac
      601 ccggccaccg gtcgccatgc cggaggccga gatgatgacg cagggatgac gcggcacgtt
      661 cagcgcctcc gactcgaccg cgttgcgcac gatgcgcagg tccggcacgc ccaggctgtc
      721 gacctcccag ccgggtggca gcaggcctgg gtcgaccgtc gccaggtagc ggcgatagac
      781 gtccagggcc cgctgcgcca tcggactgtc gaggtacacg ggcacggtgg ggacggcacc
      841 actggtcatc agctccccga cccgcaggag caccagctcg gtccggtcga tggcgaaggc
      901 ggggatgagc acggtgccgc ctcgcgcgag agtgcggcgg atggcgccgg ccagcacgtc
      961 gtccgccggg tcgggggacc gtggtgggtg agtgcggtcg ccgtaggtcg actcgacgac
     1021 gatgacgtcg gccgacggag ggttctcgcg gcgaggcacc aacgggtggt ccgggcggcc
     1081 gaggtcgccg ctgaacagca cggttgaccc tgctgcgtcc acgagcgcgc tggccgcacc
     1141 gaggatgtgc cccgccgact gaagggtcac ggtgacgtcc tcggtcagcg ggacggcgtc
     1201 gtgcagcggc accgggcgca gcagggggag ggcctgctcc acgtctcgct cgtcgtagag
     1261 gggcaacgcc ggacggtgtc gggagaaccc cgcctggttg gcgtaccccg cgtcctcctc
     1321 ctggatgcga gcgctgtcgc gcagcacgat cgccgcgagc tcagccgtct ggtccgtgca
     1381 gatgaccggc gccgccaggc cgtcgcgaac cagcctcggg aggtagccgc agtggtcgag
     1441 gtgcgcgtgg gtcagcacga caccgtcgag gctgtcaggg tcgaccggga gcacgtccca
     1501 gttgcgctgg cgcagctctc gcagaccctg gtagagacct gcgtcgacga gcacccgaga
     1561 ggctgcggtc tcgacgagga agcggctgcc ggtgacggtg ccggcagccc cccagaacgt
     1621 caacgtgggg ctgctcacgc ctccatcgtc ctccccgcgt gggtccgggg acagtggtgc
     1681 cgagggactg gttcctcaca ccgcctcgcg acccgggcgc ggcgcacccg acgacgaagg
     1741 gccgcaccgg atgccggtgc ggcccttcgt ggtcaggctc tagctggcgg tcgtgcccga
     1801 ggcactgctg ccgctgccac cactggtgcg gcgacggttg cgggtgcggc ggggacggcc
     1861 ggcgccctcc gcgggtgcgt caccgtcacg cgacgacggg cgagcggcat ccgatcgacg
     1921 gtcaccgccg cggccgcggt ccccgcggtc gttgccacgc tcgttgccgc ggccgcccga
     1981 cgggccccga cgaccggcac ccttgccggt ctcgccgagg tcctcgaggg tctcggcgcc
     2041 gaggccggcg cgggtctgct gggcgcgtgg gaggcggccc ttgctgccct cggggatgtc
     2101 gaggtcggtg aacaggtgcg gtgagctgga gtaggtctcc tgcggctcgg gcatgccgag
     2161 gtcgagcacc ttgttgatca tcgcccagcg gtgcaggtcg tcccagtcga cgaacgtcac
     2221 ggcgatgccg gtgttgcccg cgcgtgccgt gcggccgatg cggtgcaggt aggtcttctc
     2281 gtcctcgggg cactggtagt tgatcacgtg ggtgacgttc tcgacgtcga tgccgcgtgc
     2341 agcgacgtcg gtggcgacga ggatgtcgac cttgccgttg cgaaaggccc gcagcgcctg
     2401 ctcgcgggcg ccctgaccga ggtcgccgtg gatggcggcg gcggcgaaac cgcggtcggc
     2461 cagctcgtcg gccaccttgg ctgcggtgcg cttggtgcgg ctgaagacga tggtcaggcc
     2521 gcggtccttg gcctggagca tgcgggccag catctcgacc ttgtccatgg cgtgcgcacg
     2581 gtagacgaac tgctcgacgg ccttgaccgt gtgcgcgttc tcctggtcgt cgcccatggc
     2641 gcggatgtgc gtcggctggc tcatgtagcg ccgggccagg gccacgacgg cgccgggcat
     2701 ggtggcggag aagagcatcg tctggcgggc ggccggggtg agcgagagca gcttctcgac
     2761 atcgggcagg aagcccaggt cgagcatctc gtcggcctcg tcgaggacga cgatgcgggc
     2821 gtggccgagg tcgaggtgac cctgcttcgc gaggtcgatg agccgaccgg gggtgccgac
     2881 gacgacctcg acaccgcgct ggagcgcttc gacctgcggc tcgtaggcgc gaccgccgta
     2941 gacggtgagc acgcggatgc cgcgcttctt gccggcgcgc tcgaggtcgc tggcgacctg
     3001 cacggcgagc tcgcgggtcg gggccacggc gagcgcctgg ggcttgccgg ccgcgggcat
     3061 cgaggcgaag gcctcgtcgc gcggggacac gacgcggttg agcatgggga tgccgaagcc
     3121 gagggtcttg ccggtgcccg tcttcgcctg gccgatgatg tcgtgcccgg acagtgccac
     3181 cggcagggtc atcgcctgga tggggaaggg gctcgtgatg ttgtgctcgg cgagcgcctc
     3241 gacgatctcg gggtggatcg ggaagtcgga gaaggagggg gcgggcgcgg cctgcgcctc
     3301 aggagccgtg gtgctggtga cgtcggtcaa cggggtgatc tcttccggtc ggcgggcgcg
     3361 ggcgcgccca cgcaaggctg cgggccgatc ggaggtcttc ggggagcggg ccgcgccggc
     3421 agggccgggc tctcgccacg tcagggaaac agtcttcgtg ccgaccgggc accgtggaca
     3481 gcggccactc taccgttagg ctcgcgattc atggagcaca tgtcgaccga ggaccccgcc
     3541 tacaccgctg ccgtgtccga cctgctgggc gtcctggcct acggcgagct caccgcgttc
     3601 atccgcatgg cggtcgactc cgacctcgcg ccgacgttgg cgctgaagtc gtcgatggcc
     3661 ggcctcgcac acaccgagta cgtgcagtac tcgcagctca tcgagcacat gggtgctcgc
     3721 gggatcgatg ccgaggccgc gatggcgccc ttcgtgacgc cgttcgcggc ctaccacgag
     3781 cgcacgaagc cccgggactg gatcgagggg ctcgtcaagg ccttcgtcgg cgacggcatc
     3841 gccaaggact tctaccgcga gatgagcgcc ttcgtcgatg acgagacccg cgccgtcatg
     3901 ggcctggcgc tcgacgacga ggggcaggcg gacttcgtcg tcggcgtcgt gcgcgacacc
     3961 ctgaagaccg accgcacggc cgccggacgg ctgtcgctgt gggggcggcg cctgctcggg
     4021 gaggcgttgt cgcaggctca ggcggtggcc gtcgagcgcg acgccatgtc cgcgctgctc
     4081 gtcggcggag gggtcgacct ggccgaggtg gggcagatgt tcgcccgcct gacggagaac
     4141 caccagaagc ggatggccct gatgggcctc acggcctgag cgaaacaacc agtcacccgg
     4201 cacagacccg cacttattgc acgagagcca cccggatccg ggtggctctc gtgcaatgag
     4261 tggggttggt gcggtcagac cgacttcttg gcggtgacct tctcgtagcc gatgacgagg
     4321 gcagcggccg cggcgatgga gatgaaccag cggatccagt cgataccgcg ggtgggaccg
     4381 ccgccgaggg cttcccacac ggcggagccg atcagggccc cggccacgcc aagcgccacg
     4441 gtgagcagtg cgctgatctg ctgcttgccg ggcagggcca ggcgggccag catgccgatg
     4501 accgctccga agatgatcgt gccgatgatg gcgccgagag ttcccatgac aggttccttt
     4561 cgatcagggt gtgcggggac gggtggtgaa cggggtcagg gggtgaaccc gacgcgggcc
     4621 ttgtcggagt cgccgacctg gatccagccg atgaccgagc cggggacgaa gaccgagcga
     4681 ccgcgggagt cggtgaggcg cagcactccg ccgtcggcga gagccttctc cacggccgcg
     4741 gtgacctcgg cggtgggtga gtcggtctcg atcgtcagct cgcgggcgac gttctggaca
     4801 ccgatggtga cctccacggg cgcacacctt ccttcagggt tggggtgcgg cgtcaccgca
     4861 ccactgtctg gatcaggcta acccgcgcgg gcggggagcc attccggcat ccgccgtggg
     4921 cgaacacgaa acacctcgcc cggccagctg gcgacggcca gccggcgacc gccgggtcag
     4981 cgcagtctca ggcctcgacc ttggggaacg accccaggcc acgccacgcg agggagctga
     5041 tcatccgcgc ggcctcggcc tcggggacgt cgccaccctg tgccagccag tggcgcgcgg
     5101 cgacctgcgc cataccggcc agcgcggacc ccaggagcag ggcacgctcg tcgtcggcgc
     5161 cggtgtcctc ggcgatgacc tcggcgatcg cctccgcgca gttcatctcc acggcatcca
     5221 accgggcccg cacctggggc acgctcgtga ggtcggactc gaagaccatc cggaacgcgg
     5281 cgccggcgct cgtcacgaac tggaagtagg ccgcgacggt gcgctcgacg cgaacctcgt
     5341 tgtcgccgcc gacctccagg gccgcccgca ccagcgactc gagggtgtcg cagtgcttgt
     5401 cgagcagggc caggtagagg tcgagcttgc ccgggaagtg ctggtacagc accggcttgg
     5461 agacgccggc acggtcggcg atctcgtcca tcgcggcggc gtggtagccg gactggacga
     5521 acacggcctg cgcggcgtcg agcagctgcg cgcggcgctc gctgcgcggc atccgctggc
     5581 cgcgtgtggg catgggggtg ctggtcatcg aggtccttcg ctccgggcgt gaggtcagca
     5641 tcctacggac gagtaacgcg gtccaccggg acggcccgca ggctggttgt taccctcgcg
     5701 ccatgtcgcg tcccccgctc gtgcccccgg gacccgagct caccgcccag gagcgcacgc
     5761 gctacgcccg acacctcctc ctgcccgatc tcggcagcga cgggcagcgc cggctgcgcg
     5821 ccgctcgcgt gctcgtcgtc ggcgccgggg gcctgggctc tccggcgctg ctctacctcg
     5881 ccgcagcggg tgtcggcacc atcgggatcg tggacgacga cgtcgtcgac ctcaccaacc
     5941 tccagcgcca ggtcgtgcac ggggtcgccg acgtggggcg cccgaagaca agctccgcgg
     6001 ccgagtcggt gcgcgcgatc agccccgcca ccgtggtcat cgagcacacc gcgcgcctcg
     6061 acgagagcac tgccctggcc gtggtcgctg actacgacct cgtgctcgac ggcgccgaca
     6121 acttccccac gcgctacctc gtcggtgacg cgtgtgcgcg cctcggcatc ccccacgtct
     6181 gggggtcggt gttccgcttc gacggccagg tgtcggtctg gtgggcgggt gaggggccct
     6241 gttacgcctg cgtgttcccc tcgcaaccgg cgccggatgc cgtgccgtcg tgtgccgtcg
     6301 gcggcgtcct cggggcggtg tgcgcgagcg tcgggtcggt gatgggcacc gaggcggtca
     6361 agctcatcac cgggatgggc gagcccctcg tcggacgcct gctcgtgcac gacgccctcg
     6421 gccagacctg ggacgcgctg cccgtggcgg ctgatcccgg atgccgggtg tgcggctccg
     6481 gcgcggacgt ggcgcgcccg ctcgggtggg cgaccggcat ccggactgag ggggcggatg
     6541 ccggtgcgtc gccacctgat cggcccacca tcagcgtgga tcggctgcgc gaggaactgg
     6601 acgccggtgc cgtggtggtc gatgtccgag aggcgggaga gcgcgagatc gtcgccctgg
     6661 cggaggccgt ctgggtgccg atcggcgacc tgcgggcggg tgccggagtg cctggtgtcg
     6721 atgcgggggt ccgggtgtac gtgcactgca agtcgggcgg ccggtcggcc gaggcggtgg
     6781 acctgctgcg agcgcgcggg gtggatgccg tgaacgtcga gggcggcatc ctcgcgtggg
     6841 tgcgtgacgt cgaaccccac ctgccgacct actgaacgca gggcgtacgc gaggggcgcc
     6901 gcgctcttcg gtgcgtccgt cgtggcgcag tgtgcgtcat gggcgccctc gtggtgcggt
     6961 cgtgctcccg atgggtgggc cttgctcccg atgcagatcc gttctccggt ggtgcgtcgc
     7021 tgcagcactg caccaccgga gtacggactc accagcggag nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn ccggtggtgc gtcgctgcag cactgcacca ccggagtacg
     7201 gactcaccag cggagcaagg atctgcgacg ggtgtgctga gctgccacgg tccgggacac
     7261 tggccaccat gaggaacggc gaccggacac agttgctgcg cgtgcagaac ttcatgctct
     7321 ccacagacgg gttcggaacg ggggaggggc tcagcctcga gcgtcccttc ggccacgccg
     7381 accccgcggc gctggcgtcc tgggccggcg ccacggcgag ctggccgaac cgcaccgacc
     7441 cgggcgggac ccgtggcctg gacgactact tcacccgcga cttcagcacc aacatcggcg
     7501 ccgagatcat gggccgcggc aagttcggcc cgcagcaggg gccgtgggag gaccttcagt
     7561 ggcagggctg gtggggtgaa gagccgccgt tccgcactcc ggtgttcgtg ctgacccacc
     7621 acgtgcggcc gagcatcacc ttgtcggaca cgaccttcca cttcatcgac gccgacccgc
     7681 acgaggccct gcgccaggcc aaggaggccg ccgacggcaa ggacgtgcgc ctcggcggag
     7741 gggtgaggac cgtgcgcgag ttcctcgagg ccgacctcgt cgacacgatg cacgtggccg
     7801 tcgctccggt cgagatcggc cgcgggggac ggctgtggga gtcgcccgac gagctgctcg
     7861 accgctttca cctcgaggtc gtgcccagcc cgagcggcgt cgtgcaccac ctcttctggc
     7921 gccgctgacc tcacgggtca gctggtgtcg gaggggcgtg gtggaatccc ggggtgctcg
     7981 tccttcgccg ccccgacctc gccgaggccg ctgcccccgt cctcgacggt tcgcagcagg
     8041 ccgcggtcga gcaccgggct gccgtcgttc gggtgctcgg tgcacccggc accggcaagt
     8101 ccacggtggc cgtcgagcgc atcgcggcgg cggtcgccga ggggctgact gctgaccagt
     8161 gcctgctgct cgccccgacc cgacgtgcag cagcgcgcct tcgggatgcc gtcacggctc
     8221 gcctcggcgg cacgaccacc acccccctgg cgcgctcgca ccagtccttc gggttcggcc
     8281 tgctccggca ggcggccgcg ctcgccggtg acccgacccc gcgactgctg agcggccccg
     8341 agcaggacgt catcctcggc gagctgctcg ccggccacgc cgccggcacg gccccggcgc
     8401 cgcagtggcc tgccgagctc cacctcgccc tgcccacccg caccttccgc cgcgagctgc
     8461 gtgacctgct catgcgtgcc gtcgagctcg gtctcgagcc tgaggcgctc gcggagctcg
     8521 gtcgcgacca cggccgaggg gagtgggtgg cggcggccca cgtcctgcgc gagtacgacg
     8581 aggtgaccgc gctgtccgcc gccggggcgt tcgacccggc gtggatcctc ggcgcggccg
     8641 ccgacctcgt cgaggacgac cccacctccc tgccccagag cctgcgcctc gtggtcgtcg
     8701 acgacgccca ggagctcacc ccgccggggc tgcggctgct ccaggagacg gtcacggcca
     8761 gtgctgccca gctggtgctc atcggcgacc cggatgccgc caccgagacc tttcgcggag
     8821 cagaccctcg cctgttcctc actgcctggc cgcaggcgga cacgatcacc ctcggcgcct
     8881 gccaccgttc aggagcgccg cttcgcgccg cggcctctcg agtcgtcggg cacatcggtg
     8941 tcgtcggcgg ggtggcccac cgggccgtgg accccgaccc gcgcgacggg cgagtcgaga
     9001 cgcacctcgt gcgcacggca tcccaggagg ccgggtacat cgcgggtcgt ctgcgcaccg
     9061 cgcacctcat cgacggcgtg ccgtggtcac gcatggccgt catcgtgcgc ggcgggtccc
     9121 gcacggccac cctgcgccgc gtgctgcgca gttccggcgt gccggtcgac acgaccaccg
     9181 ctgacctccc cgtggccgac gaggccgccg tgcggccgtt cctcaccctg ctgacctggg
     9241 cgctggaggt gatcgccggt ggtgagcacc tcgatcccgc gcaggtcgtc gacacgctca
     9301 cctccccggt cggtggggcg gatgccgtgt cgctgcggcg gctgcgccgc tcgttgcggc
     9361 gactcgagct cgacggcggc ggtggccgac cgagtgacgt gctgctcgcc gaagccgtgc
     9421 tcgcgccggg cgccctcgac cacgtcggcc cggagggcca gcccgcacgc cgggtgcacc
     9481 gggtgctggc caccgcggtc gacgccctgc gcgaggggga cggggtcgag gtcgtcctgt
     9541 ggcggatgtg gtcggcgagc ggcctggccc agccgtggcg cgacgccgcc ctcacgggcg
     9601 gctcggcggg tgggcgcgcc gaccgtgacc tcgacgccgt gctcgggctc ttcgatgccg
     9661 cggcccgctt cgtcgaccgc ctccccggcg cctccccggc tgacttcctc gatcacgtgc
     9721 tcgctcagga cgtcgctggc gacagcctcg tcgcgagggc ccccgcaggt gagtcggtcg
     9781 ccctgctcac gcccgcggca tccgccggtc gcgagtggga cctcgtcgtc gtcgccgggg
     9841 tgcaggaggg ggtgtggccc gacctgcggt tgcgcggatc gttgctcggc tcgaccgacc
     9901 tcgtcgacgt gctcgccggc cgtggtgatg acccccgggc ggcgcgtgcg caggtgcgcc
     9961 atgacgagac ccggctcttc cacgtcgccg tcacccgggc ccgtgagcac ctcgtcgtca
    10021 ccgccgtgcg tggtgaggac gagcagccct cacccttcct cgacgtcatc gacccgctgc
    10081 cccaggcgcg caccttcacc gacgtcccgc gcccgctgac actcgcgggc ctggtcggtg
    10141 agctgcggcg cgaggtcgcg ggggaggacc cggcccgccg aggagccgcg gtcacgaccc
    10201 tcgcccgcct cgccagccac cacgtgccag gcgccgaccc cgcgcagtgg tgggccctgc
    10261 gcgacgtcag cgacacccgc ccgcgccgtg cgcccgacgc accggtcgtg atctcaccgt
    10321 cggccatcga cccgttcatc cggtgtcgcc tgcagtgggt gctgcgtgcc gcgggcggtg
    10381 acgggccctc gatgggcgcg caggacatcg gcacgctcgt gcacgacgtc gcccacgagc
    10441 tcggtgacac cgatgcggcg acctacgccg ccgaggtcga ggcgcggtgg gggcgactcg
    10501 ggctcgcccc cggctggctc agccgtcgca gcctggccca ggcgaccgcg atgaccgacc
    10561 ggctggcgcg ctacgtcacc gagtcggatg ccgccgggtg gcgccgggcg gcctccgagg
    10621 cccgcatcga ggccgcgatc gggcgagccc gcatcaccgg tcgggtcgac cgcgtcgagg
    10681 tgtcgcccac cggccaggtg cgcatcgtcg acctcaagac cggcgccagc aaaccgagcg
    10741 tcgcagaggt gtcccggcac ggccagctcg gtgcctacca gctcgcggtg cagcacggtg
    10801 ccctgcccga cgtcggcacc gagccgggcg gagcagcgct gctccacctg ggcaaggccg
    10861 ccaacctcac caccaccgtg caggtccagc agcccctcga cgccgatgac gacccgcagt
    10921 gggcccatga cctcgtcgaa caggtggcgc aggagatgtc cggcgccgtc ttctccgcca
    10981 cccccggaac gaagcagtgc ggcacctgcc aggtcaagga ctcctgcccg gccctggccg
    11041 aggggaggcg gctgtgacgg tgccgcggtg gagtgccgtc gcgctcgcgc aggcgctggg
    11101 ccagcacgcc ccgacgcctg agcaggctgc ggtcatcgag gccccgctgc gccccctgct
    11161 cgtcgtcgcc ggggccggtt cgggcaagac cgagacgatg gcggcgcgag tggtctggct
    11221 ggtcgccaac gacctggtgc gccccgacga ggtcctgggg ctgaccttca cccgcaaggc
    11281 cgccggtgag ctgtccgagc ggctctccgc gcgactggcc accctgcgtg aggccggcat
    11341 ctgggtgccg caggaggagg acggcgccgc cgtgctcgac gatgccccga cggtctcgac
    11401 ctaccacgcc tatgcgggcc ggatcgtgcg cgagcacggc gttcgcctcg gggtggaggc
    11461 cgagagccgc ctgctcagtg aagccgccgc ctggcaggtc gcccacgagt cggtcgtggc
    11521 ctatgacggg cccatggacg aggtcgagaa ggcggagtcg accgtgacga ccgcggtggt
    11581 cgacctcgcc ggtgagatgg ctgagcacct cgtcgacgtg gatgccgtgg ccgcgcacct
    11641 cgacgaggtg gtcgccgccc tcgagctcat cccgcggggt gaggcctcga cgaagaaggc
    11701 ctttcccaag gaggtcaggg acgccatcgc cgtgctgcgc gagcggcgcg cgatcctgcc
    11761 gctcatcaag gacttccagg ccctcaagcg ctcgcgcgac agcatggact tcgccgacca
    11821 ggtgcagctc gccgcccgca tcgcgatgac ggtgaagcag gtcggggtcg gtgagcggtc
    11881 ccggttccgc gcggtgctgc tcgacgagtt ccaggacacc tccgaggccc agctccagct
    11941 cctgcggtca ctgttcgtgg ctcccggtgc ggcggtgccg gtcaccgccg tcggcgaccc
    12001 ccaccagtcg atctacggct ggcgcggggc gagctcgacg acgctgcacc gcttccgtgc
    12061 cgacttcgtc gacgatgccg ggccgaccga ggtgctcccg ctgtcgacga gctggcgcaa
    12121 cgaccgagcc gtgctcgagg tcgcgaacca cgtcgcgcag ccgctgaccc gtgcgacgtc
    12181 ggtgccggtg caccggctcg gggcccgcac ggatgccggc cccgggcacg tcgcgacggc
    12241 tcggttgtcg acgctggagg acgaggccgc gcacgtcgcc gactgggtgc gggcccggct
    12301 tcgtcggccg gggcgcgcca cggcggccgt gctgtgtcgc aaacgctccc agttcgaccc
    12361 ggtcatcgag gcgttggagg agcgcggtat cccctacgag gtcgtgggtc tgggtggatt
    12421 gctccacacg ccggaggtcg ccgacctcgt cgccctgctc tgggtcgtgc aggacccgag
    12481 tcgtggtgac cggttgatgc gcctgctgac cgggccgtcg tgccgcctcg gtgccgccga
    12541 cctcgacggg ctcggtgcgt gggcccgaga gcggcagcgc accggccgtg accggaggcg
    12601 caccgacctc tcgcgcgacg ccagcgagca ggccagcatc gtcgaggccc tcgacgacct
    12661 cccacccgcc acctgggtcg ggccggaggg ccagtcgctg tcgcccacgg ccctcgcccg
    12721 tctcaagggc cttgccgaca ccgtgcgcca cctgcgcacc ctcaccggcc tcggtctggc
    12781 cgagttggcc gccgaggcgg aggtggccct cggtctcgac atcgaggtgc tggcccgcac
    12841 cacgtggagc ccgggcgccg cccgtgcgca cctcgacgcc ttcgccgacg tggcggcgac
    12901 cttcgccgcc agcgctgacc ggtcgaccct cggcgggttc ctcgcctggc tcgacgcggc
    12961 cgtcgacgag gagcgcggcc tcgacctcgg ctgggtcgag gcgcgccccg atgccgtgca
    13021 ggtgatgacc gtgcacgcgg ccaaggggct ggagtgggac gtcgtggccg tcccggggct
    13081 cgtggagtcg tcgttcccgg cccactccgg cacctcgacg accgtcaagg acggcgcgtg
    13141 gaaccactcg accccgaccg acaagggctg gctggccggc ctcgcgaccc ttccctacga
    13201 cctgcgcggc gacgccgacg ggctgccgca cttctcctgg cgcggcccgc acacgtggga
    13261 caccatcgcc ggcacgtccg gcgagtacca ccggttcagg ggcgcggtgg cggcccacgg
    13321 catcctcgag gaacgacggc tggcctacgt cgccttcacc cgggctcgtc acgacctgct
    13381 cctgaccagc cacgtgtggg gcacggccgg cactcctcga ctgacgtcgc gcttcctgct
    13441 cgaggtgcgt gggagcgggc tggctcaggc gtccggcccg tgggtcgacc tgccgcccat
    13501 cgaccccaag ccgcagaacc cgcgaacggc cgagcaggtg gacgtcgcct ggccgacgca
    13561 ggaccacgtg ccccgcaggc aggggctgct cgagcccgcc cgggccatcg cgggtgccgt
    13621 ggccaccctc tcgcaggcac ccgacggttc cggtgtggcc gctccccgcg ggccctggga
    13681 cgaggaggtg acgatgctgc tcgccgagcg ggctgcgcgc ggtcgcggtg ctgacgtcga
    13741 cgtcgtgctg cccgcgcacc tgtcgacctc tgccctggtc gcgctcgccg aggactccgc
    13801 ccggttcacc ctcgacctcc ggcgccccat gcccaccccg ccggccctgt cggcgcgccg
    13861 cggaacggcc ttccacgcct gggtcgagga gcactacgcc cgggcagcgt tcgtcgacat
    13921 cgacgagctg ccggggtcgg ccgacgacgg agccgtcgac gacgaccttg ccgcgctgcg
    13981 cgccagcttc agcgcgagcg agtgggcgga tcgcacgccg gccgaggtcg agacgagcgt
    14041 cgagaccgtc ctcgacggca tcgccgtgcg tggccgcatc gacgccgtct tcgaggaggt
    14101 cggcgacgac ggccagccct cgtgggtcgt cgtcgactgg aagaccgggg cggcgccgag
    14161 cggatcccgt gcgtcagcgc gaacgcttca gctcgcggcg taccgcatcg cgtgggcgcg
    14221 ggtgcgcggg gtgccggtcg agcgggtgcg cggtgcgttc ttccacgccg cgacgggcga
    14281 gacgacctgg ccggacctgc cgggggtcga cgggatcact gcggtgctga gggccgctcg
    14341 tccgggctga cctcgccgga cccgagttcg tccgcgtcct cctcgtcggt ctcggccagc
    14401 gcggcatcct ggcccggcgc atggtcttcg tccggctcgt catcctcgga gtgctccagc
    14461 tcgagccacg cggggacctc gtcaccggcg gcgacgggaa cagtgacggg aaccgcgacg
    14521 ggaaccgcga ccgtgtcgtc gcggtccgtc tccacgagtt cggaatccac cggctcggtt
    14581 tccatgaggt cggggtccac gagctcgggc agctcctcgt cgtcctcgtc gacgacgagg
    14641 gcggggggtg gggtcgggcg cgtccggatg ccgatcggcg ccaacgaggt ccggcggtag
    14701 tcgtcgggct cctcgccggc gtggacggcg tcgtcgaggc ggcgaagctg ggtcgtcagc
    14761 acctcgacgg caccggcgtc ctgccgggac agggcggtca tgagggtgcc gagcaggccg
    14821 agttcggcga cgaggcgggc gcgcacgatg aggttggtgt cggggcgttc gacccgtgcg
    14881 tgggcgtagg cctcgagcac agtctcgacg gcctcggggc tcgcgtccgc gacgagaggg
    14941 gcgaagtcat cggcgggatc ggcgaccttg gcgtcctccc agccggtcat ggcgcggatg
    15001 gtgcccgtcg acgcgtcgga gtcgtcggtg aagacggcca ggacctggtc gccggtgagg
    15061 tctccgtggg tggcggtcgg cgcgaaccgc cagagggtga cgtcctcgag ggccttctcc
    15121 caccgggtca acagcgtcgt cggcacgcgc ccggtctcgg cagcgcggtc gagctcggcg
    15181 agccggcggc tgcggtaggc gtcggcgtcg tacgacggca ggcccgcctc gtcgtagatg
    15241 gccgggtcgg tgttgtgcag ggcggcgatg gcccggccga gctcagcggc caggccgggg
    15301 ccggcaggca actcggcgaa gtccaggttg tgcccgggca ggtacgggta gacggcagcg
    15361 cgcccgccct cggccagggc gacgaacccc ttgggtgcgg ggaccgcgaa cggtagccgt
    15421 cggccgagca gggccagcag ggcgacggtg cggtccatct cggcacctgc gacgtcgctg
    15481 cgggggaccc gcaccaccca tctgcggtgc tcggtgtcct ggatgaacgc gacgtcgaag
    15541 gactggtccg gggtgctcga cagggcctcc acgctcaccg ggtcgagccc ggagaccgcg
    15601 gcgctggcga gggcggcgag ataggcgggg ctgcggtcca cgcgaccacg gtatgcgctc
    15661 gaccccccat aggctgggac gcgtgcccga ccgtgccgcg atcctgcctg acctcgccct
    15721 cagccggtcc gtcctcgaca gacgcggcga cctgcgggcc gacccctctc tgctgacccg
    15781 gctgttggct gacggcgaca cccgcgtgct gcgcctggtc ggtgaccgcg ccgccgtcat
    15841 cgctggcggg agcaccggtg acggcagcga cgaggtgacc cgggtggtgt tcgacgcgcc
    15901 gacccccgaa gacctcgacc ggctgggcct ctacctcggg gatgccgcgg gggtctcgta
    15961 cgtcggcgtc gtcgccgacg acctcacggc atccgtcgag ggtgacgatg cgtggcgcac
    16021 gctgcgccag gtcggtgcca ccctggccga cctcgacgcg cacgcgctcg tcaccacgct
    16081 ggcgctcgcg aactggcacc ggtcccacac ccactgcccc cggtgcggca ccccgaccga
    16141 accggtgctc tcggggtgga tccggcggtg cccggcggac tccagcgatc actatccgcg
    16201 caccgacatg gcggtcatca tggcggtcgt cgatgctgac gaccggttgc tcctcgcgcg
    16261 gggcaagggc tttcggggca acgggatgtc ggtgctcgcg gggttcgtcg agccgggcga
    16321 gagcctcgcg gcggccgtgg ctcgtgaggt cctcgaggag gtcggggtgg ccgtcaccga
    16381 cgtcacctac ctcggtgacc agccctggcc gttcccgtcg tccctgatgc tcgggttcac
    16441 cgcccgcgca ctcgacaccg acctgcacct gcaggcagag gagatcgagg cggcacgctg
    16501 gttcagccgc gatgacctcg tcgcggcgtt ggcggacggc tcggtcgtca tctcggggca
    16561 gctgtcgatc tctcggcgga tcatcgagca ctggttcggt gggccgatcg actcaccgga
    16621 catccccctg cgctgaccct cgtcgttcgc caagtcccga tgccgggggt caggtgaggc
    16681 gggagcgcac ctcggcgatc gtcgggttgg tcgcggcgct gccatctgcg aagagcaggg
    16741 tcggcaccgt ctggttgccc ccgttgaccg acatgacgaa ctcggcggtg ccgggctcgt
    16801 gctcgatgtc gacctcggtg aaggagatgc cctcgcgctc catctgcgac ttcaggcggc
    16861 ggcagtagcc gcaccaggtc gtcgtgaaca tcgtgacggt gcccggcgcg ggcaggtggg
    16921 tgacggcggc gtcgctcatc gtggtccttc ggtggtgaac gggtgggctc ggtggaggtg
    16981 aacgtcgcac cacggccgat ccttcccggc ctgcggtgcc atccctgccg tccagacctt
    17041 ccggccccgt cggtggcccc acctaggatc atgggcgatg agcagcctgc cagtgcaccc
    17101 cggagccgac gcggtcctcg atgcactcga ccccgagcag cgcgaggtcg cggctcaccc
    17161 gagtggcccg atgtgcgtgc tcgccggcgc cggcacgggc aagacccgag ccatcaccca
    17221 ccgcatcgcg tacggcgtgc tgtccggggt gtaccaaccc cagcgggtcc tggccgtcac
    17281 cttcacggcc cgggccgccg gtgagatgcg cacccgcctg cgcgacctcg gggtgggcgg
    17341 ggtccaggcc cgcaccttcc acgcggcatc cctgcgccag ctgcactatt tctggcccca
    17401 ggcgatcggc ggtgcgtcgc cggaggtgat gccgcacaag gccagtgccg tcgccgaggc
    17461 cggctcccgc ctgcggttgc gcctggacag ccccgccatc cgtgacctgt cctccgagat
    17521 cgagtggtcg aaggtgtcgt tgctgactcc cgagtcctac gcggcggcag ccgccgcagc
    17581 acgacgtgaa cccccggccg gtctggatgc cacggcgatg gcccgcctca tcgcggccta
    17641 cgaggaggtc aagaccgacc ggggcgtcat cgacttcgag gacgtcctgc tcctcatggt
    17701 cggcatcctg cgcgagcacg aggaggtggc ccgggcggtg cgcaaccagt accggcactt
    17761 cgtcgtcgac gagtaccagg acgtcaaccg cctccagcag accctgctcg acctgtggct
    17821 gggcgagcgg cgcgacatct gcgtcgtcgg ggacccggcg cagacgatct actccttcac
    17881 cggggcctca cccgagcacc tgctctcgtt ccgcacccgc caccccgacg cccgcaccgt
    17941 cgaactcgtg cgcaactacc gctccacccc gcaggtcgtc accctcgcca acctcgtcct
    18001 gcgcggcccg ggcgggcagc gccgatccgg ctcggtcgag ctgcgtgccc agcgcgccga
    18061 cgggcccgtg cccgagctcg tcgtggccga caacgacccc gacgaggcgg ccgccgtggc
    18121 gacgcgtatc gcgtcgttgc tggatgccgg gcacccaccc tccgaggttg ccgtgctgtt
    18181 tcgcaccaac ggccagagcg aggccttcga gtcggcgctg gccgaacgcg gcatccccta
    18241 cctcgtgcgc ggcggcgagc gcttcttctc ccgcaaggag gtgcgcgacg cggtggtgct
    18301 catgcggggc gcggcgcgct ccgacgacgg gtcggtgccg ctgcccgacc ttgcccgtga
    18361 cgtgctgctc ggggccggct ggtcccggga ggcccccacg agcgggggag cggcgcgcga
    18421 gcgctgggaa tcgttggccg cgctcgccgg cctggccgac gacctcttcg cgaccgaccc
    18481 gcaggcacgg atgccggccc tcgtgcgtga gctcgaggaa cgggccgcgg ccagccacgc
    18541 acccacggtg cagggcgtga ccctggcgtc cttccacgcc gccaaggggc tcgagtggga
    18601 caccgtgttc ctcgtcggct gctccgacgg gttgctcccg atcaccatgg ccgacacccc
    18661 ggaggccgtc gaggaggagc ggcgcctgct ctacgtcggc gtcacccgcg cccgcgagcg
    18721 actcgtgctg tcctacgcag gggcgcgcac gcccggggcc aaggcgactc gacgcccctc
    18781 ccggttcctc gacggcacct cgagcgtgct cggcgacggc gcccgctctc aggcccgtcg
    18841 acgcaaggcg cccggaacgg gtggcggggc cggtggtggc aagggcaagc cggcggttcg
    18901 ttcgacgtgc cgcacgtgcg gggctgatct cgggacggcc cgcgaacgga ccatcggccg
    18961 atgctcgacc tgcccaccca cgatggatga gcggctcttc gaggccctgc gcgagtggcg
    19021 cctggagacc gcccgcgagg ccgacgtccc ggccttcgtc gtgttcaccg acgccacgct
    19081 gaccgcgatc gccgagaagg tgccgcgcga cgtcgccgcg ctgtcacgga tcacgggggt
    19141 cggcccggcc aagctcgagc ggtacggcgc cgccgtcctg agcctcctgg aagaattttc
    19201 tgaagcccaa tagccctgtg cagcagtact tttcgagctg gtggcggctg ctccgcgcct
    19261 ggcatgccga taaatgcgtt gccccggcca cgagggctca cctaatctga caggcgtgcg
    19321 cccggtcggg tgtacgcaca ccgcgagttc agtgaggagg gttggccctg atggagacga
    19381 cgatgaccaa ggacctggcg atgagcgctg tccgcgagat ctgcgcgcgt ccctggcgca
    19441 gcgccaacac cgctcacgca ggcaccttcg gctccgacca ggggcgcact gtgtcctacc
    19501 ccggcagcgc cacccgtgac gacaagctcg tcgccggtgt gttcgccgtc ctgggcaacg
    19561 ccctggccca gccgttcggg tacccccgcc ttgaggcccc acctgtctga catctgacga
    19621 caggtcacct caaggccgcg gatcccgaca cgggatccgc ggccttcgtc attcccagtg
    19681 acaccactca ctcccgctga caccactcgc agccatccgc acccagacca caccccgagg
    19741 ccaccgccat gaccaccacc gcagatcgga tcgacaccga tcgctccacg accccgcgcc
    19801 acacctggcg caccgagacg acgacgagcc gcacccggcc cgtcgaccaa ggaggtgaca
    19861 ccccgatgat gctgacccag ctgctcgacc tgcacgaggc gtccgacgcc ctcggcgaag
    19921 ccatcccctg ccgggagaac gacgccgagc tgtggttcgc cgacaccccc gacggcgtcg
    19981 agttcgccaa ggccctctgc ggcacctgcc cggctcggga cgtgtgcctc tccggggccc
    20041 tcgaccggcg tgagccgtgg ggtgtctggg gcggagagct cttcgtccag ggcgccatcg
    20101 tcgcccgcaa gcgtcctcgc ggacgtcccc gcaagaccga cgtcgccgcc tgaggcgacc
    20161 agtcaccttc gaccaccagg agatcccgat gtacttccga cgcatgcagc gcatccgtcg
    20221 caccgcgcac cagcgccgcg tccacgaggc gcagcgtcag gtgcaggccg cggccgagcg
    20281 cgagcagctg cgggcggtca tccacagcca cgtgcgatga ccccccagac cacccaccaa
    20341 ccccggcagc aggtcgaggt gataccgcac ggattcgcgg gcggtaccac ctcgaccccg
    20401 gcggcggcag cggcaccgcc cctcgccggg cccgacttct ggcgcgcggt cgccgtcgag
    20461 gtgctggcta cgctgtcgtc atggcttcgc tgacgatcgc ccaggacccg agcgccgacc
    20521 gtgtcctctc gcaggacccg ttcgccctgc tcaccgggat gctgctcgac cagcagttcc
    20581 ccatggaccg cgccttcgcc ggccccgcca aggtgctcga gcggttcggc accctcgacc
    20641 cggccgcgat cgccgccgcg gaccccgagg ccttcgccga cctgtgcgcc accccgccgg
    20701 ccgtgcaccg ctacggccgc tcgatggcgg ggcgcatcca ggccctcgcc gtcgtcgtgc
    20761 gcgacgagta cgacggtgac gcctcggcca tctggagcac cgcgacgacc ggcaatgagc
    20821 tgttcgcgcg gctcaaggca ctacccggct tcggtgagca gaaggccaag atcttcaccg
    20881 cgctgctcgg caagcagctc gcggtgcgcc ccgacggctg gcaggaggtg tgcggcgcct
    20941 acgccgagcc cggctccttc cgctcggtgg ccgacgtcgt cgatgccgag tcgctgcaga
    21001 aggtccgcga cttcaagaag gccgcgaagg ccgctgccaa ggccggggcc tgagcccgat
    21061 ggctgagcac gacgacacgg caccgacggc ggatgccggc cgaccggcat ccgacatcgt
    21121 ctgcgcgtcg tgcggtgcca cacccgacgg cgccgaggcg ctctccctgg cgcggatcac
    21181 ctgggccagg ggcgtcgaga acgggcggga cacctggacc tgcgagcagt gcagccgccg
    21241 gttcctgcgc agcatcgagg ggaagctcga ctcctcctgg tggtgaggcg ggtctgctca
    21301 gctgggggag gggggcagac gtgactcgtc gatgccggac aggtgcgcgc agacgagttc
    21361 gcgcagtggc acggtgccgc cgatctggct gagcacaccg atgccgccga gccacacccg
    21421 gtggatgagc aggtactccg gcggcaggtt gagcttgagg ccgatgagga actgggggcg
    21481 gcgggggtcc tgaagggtgg ccgcggcccc gcggagccac tcgcgggtcg gggtgaactg
    21541 gtcgtgcagg aggggttcca ccaggggttt gaggtactcg agcagcgcgt cggggtcgat
    21601 ggcgatgccc ttgcggatga agccttcctc gcgcagcacg gcttcgaggg ccacggcgtc
    21661 accggccagc gcctcggtga gcacgcgccc gagcgatgcg ggcagcccgt gcggaaggcg
    21721 gttgacggcc ccgaagtcga acacggcgag ccggccgtcg tcggtgatcc ggaagttgcc
    21781 ggggtggggg tcggcgtgca gcatgcgagc ccggttcggg gcgcgcagga ggaactcgag
    21841 gtagagggtc gcggcctcgt cgcgctcctg cgtggtgccg ttcgtgatga cgctcgcgag
    21901 gggcgtgccg tcgatccact cggtgacgat gacgtgcttg gagtccacga gcacctcggg
    21961 gatcaggacg tcctcgtcgc cgcggaaggc gttggcgaag tgccgctggt tgcgcgcttc
    22021 gaggtggtag tcgagctctt cgctcatgcg ggccttgagc tcggccatga tcggcttgat
    22081 gtcgatgccg ggcacccacg acccggccat ccgggccacc cgcgagatct ggttgaggtc
    22141 ggagagcagc gcctcggccg ctcccgggta ctggagcttg acggcgacct cgcgaccatc
    22201 gcgccagacc cccttgtgca cctgaccgat cgaggccgcc gcagccggga cgtcgtcgaa
    22261 ctcgacgagc ttggtgctgg cccagcgggg gccgagctcg cgggcgagga tctcgtgcac
    22321 ccggtcggcg cccatcggcg gcccggactc ctggagcttg gtgagcgtgg cccggtacgg
    22381 cccggccagc tcctcgggca tcgcggcctc catgaccgac agggcctgac cgaacttcat
    22441 cgcgccgccc ttgagctgcc cgagaacggt gaacagctgt tcggccgtgc gggcctgcag
    22501 ctcggcggcg acggcctcgg ccggtcgccc acccacccgc ttgcccacgc ccatggcgac
    22561 ccggccggcg tgccccaggg gcaggccgac gagcttggcg gtgcgggcga tggagctccg
    22621 cgggatgtcg gtcaccctgc cattctcacc cgcgcacacc cacagtcggc acgcacgttc
    22681 cacagacgat gggtcaaggt cacgtcggcg aggccgatct cggtcgagat tccgcccaga
    22741 gacacgtctc ctcgcagggt cgacaggacg atgacggtgg cgacggcgca ggccaggacc
    22801 accgtgcccg acggcgtgtc ggatgccggg tggtcgggcc ggatgccgtc ggcaccgggt
    22861 gccccagcgg ccgcacgggt gcgtggcgac gcaccgcccc gggcctcggc ccaggcgtcg
    22921 ccgcagcagc gcaggcacgc cgtgctgccg ggaaggacga gcgggccgac gacgacccgg
    22981 ccggcaccga ccaccactgg caggtgaggg atgccgcggg cctgccacgg ggtgcccgcc
    23041 cagcgagggg ggcggtcacg ggcgaccacg acgaccgcag tcggggccgg ctcgccctcg
    23101 gcgagggcga gctcggcacc gtccgcagcg tgcgctcccc ccacgaccgg cacctcgcag
    23161 cggcgcaact gcgccacgag ctcgtccgcg aagaggccgg ttccgtcgac gacgacgtgc
    23221 ccggcccctg accgtccctg catctccacc cccgacctga ctggcccgac ctgactggcc
    23281 cgacctgacc ggtccgatgc gaccggtgcg accccgaccg gagccacccc ggccgccggt
    23341 gccatcctgc cctgcccgca gggctgaaaa ccgtcgtcca caggctgttc gtcgcgcagc
    23401 ccacgcaccg cagggtcccc ggacagcacg cggccccggt cccaccagga ccggggccgc
    23461 gggtggaggt tcggtcaggc cttgccgagg atgcggttga ggttggtgcc acagaccggg
    23521 cacacaccct tggccatccg gcggccgttc tcggagacga cgaccttgcc ctcggcctcg
    23581 cgcttctcct tgcacttcac gcagtagaac tcgcccttgt aggtctcgtc agccatgatc
    23641 tctccttgtc gtcgacgccc ggctgggcag ccgcgtcgat caggtgtggg tgccgcgcag
    23701 gcggcagggt ggctcaaacc ctagccacgt gcccgctgcg gatcaggcgg acgcgcgggt
    23761 cccgcgaggg ggacgcagcc actcccgcac cgcacaccac ttcggcacca tccgccccac
    23821 cccgagcgct ccgggcctag tctgcgggtg tgagcaggga ggacgtcgag gtccgccgga
    23881 gtcgccgccg gcgccgcacg gtcagcgcgt atcgagaggg cgagcgcacc gtcgtgctca
    23941 tcccggcatc cttcagcgcc gccgaggagg agcggtgggt gacccggatg ctgacccggc
    24001 tcaccgccgg tgaccgccgc cgcgctcgcg gggacgacga gctgcaggcc cgggcggcgc
    24061 acctgtcgca gaagtacctc ggggggcacg cgcggcccac gagcgtgcgc tgggtctcga
    24121 ccatgggtcg ccggtgggcc tcgtgcacac cgggcgacgg caccatccgg gtctccgacc
    24181 ggttgcgtga cgtgccgaac cacgtgctgg actacgtgct gctccacgag ctggcgcacc
    24241 tgctcgtgcc gggtcacggc ccggcgttct ggcgcctgct cgcgtcctac cccaagctcg
    24301 accgggcccg cgggttcctc gacggcttcg cgcacgccga ggggttggcc cccgggccgg
    24361 acgaggaccc tgtcgacggg ccagccgacg tcgaatcagc cgtcgagcag tcagccgtcg
    24421 agcagtcagc cggcgtcgag ggcacgcaac gcgagtgaca ccagtggcgc gagctcggcc
    24481 cgcgtgccgg cgggcagggc gtcgatcggc caccagcgca cgtcgagcga ctcctcgctg
    24541 acgacccccg ccgccccgtc gggcgcgagc gcgacgtagc gcaggtcgag gtgctcgcgg
    24601 cagtggccga aggcaccgac gagttcgtgc cggtcgagct gcacggggtc gggcaagggc
    24661 tcgagcgcac cgatcccgga ctcctcgcgt gcctcgcgcc gtgccgccgc ccacaccgat
    24721 gcgtcaccgg cctcgaggtg gccgccgaac tggaaccacg ccctggcctt gcggtggtgc
    24781 gtcagcagca cctgtgcgcg ggccgcgtcg acgaccaggc acgacgccgt gaggtgcgcg
    24841 ggtgggccgg tcttggcggc gccgtcgggg tgggcggcga gatgggcgag gtactcctcg
    24901 cgcaggcgag cctgcccggc atccggtgcc gtccaccgcg acaggacgtc gacggcatcc
    24961 gctcgaaggt gagcgaagcc gccttcgctc acgcggggtc ctcgccgcgc agcagggcgt
    25021 ccagcgctgc gtccatgtcg tcggtcgtcg gcgaggacgt gcgctcgacg tagccgaggg
    25081 ggtcgtcgag gtccgcggca gacggggcga ggtcggggtg gcgccacggg gcatcgcgcg
    25141 tggcggcgtc gccggagtcc tcgagggcgg agaacaggtt ggcggcgtca cgcaaccggc
    25201 gggggcgcag ctcgagcccg acgagcgcct cgaaggcctt ctcggccggg ccgctcgttg
    25261 cccggcgccg gcgcacggcc tcaccgagcg cgtcggcctg gggcaggtgc tcgcgggtgg
    25321 cgcgatcggt gacgacgtcg acccagccct cgaccagggc cagccaggtc tcgaggcggg
    25381 tcagggcagc ctgctgcgcg ggggagggct gcggggcgaa catccgcccc tggagggcct
    25441 gctgcacggc ctcggggtcg gaggggtcga tggagcgcac ggcctcctcg atggcgctgg
    25501 tgtcgatggt gatgtccgcc gcgtagtcgc gcaccgcggc cagcagggca ggggccagcc
    25561 acggcacggc gccgaacagc cgtacccggg ccacctcgcg caccgcgagg tagagccgtg
    25621 cctgcggttc gtcgacctcg aggccctgcg cgaacgtgcg cagcgcgctc ggcatgaggg
    25681 ccaccgtcgg ggcagggacg agggggagcg agacctcgca gccggtcagc acctcacccg
    25741 cgagggtgcc cgtgccctgg ccgagctgca tggagaacat cgagccgtgc atgcgccgca
    25801 gcatcggccc tgcctggtcg agcataccgg cggggatgcc gcccggcagc cccgggatgc
    25861 cggcgtcgcc gcggttctcg aggttgccca gctgctcgag ctgcgacccg agggcggtcg
    25921 tcacggcatc ggtgactccc gcggcgatcg gctcgacgag gtcggtccag accggcatcg
    25981 tcgcctcgac ccactcggcc gcgctccagg cgacaccgtg caggccgggc gtcgtgaggt
    26041 cggtgacggc gtcgagccag agcccggcga cctggacggc ctcggcggcc tcgcgctggt
    26101 cggcgctcgt cgcgagggtg tcgccctggg ccgagaccgt cttgcgggcc atgtcggtgg
    26161 ccagggtggc gttgacggcg tgctcgctct cgggggcgga gaacatcgcc tggacctggc
    26221 ccatgaccat cgcgagcatc tgcgggtcga ccttgtccag gcccatgccc tgcatcatcg
    26281 aggcgagctg cgggtccagg ggtgcaccac cggtgaggtc ccgcagcagc ttctcgatct
    26341 cggggggcag tgcggaggcg tcgtcggcgg ggtcgttcac gcggggctcc tggcattcgg
    26401 gtgagaggat gtcgtctcga gacaagccaa ccacgatccg gcccagatgg ttcccgccgg
    26461 tcaccgctgc cgaggagcac cattgacgac cgaccctgac ccggtcgccc tcgtcgccct
    26521 gcgcgaccag ccgttgtccg tcgacgaggt gctcgatgcc gtccggcatc cgagctgtgg
    26581 cggcatcgcc ctcttcgtcg gcgtcgtgcg cgaccacgac cacggtgcgg cagtgacgtc
    26641 gctcgactac gaggcccacc cgagcgccct cgacacgctg aaccgcgtct gcgccgaggt
    26701 gctcggccgg tcgcaggccc ggcgggtcgc cgccgtgcac cgcacggggt cgctggccat
    26761 cggtgacctg gcggtcgtcg tcgcggcatc cgctccgcac cgcgggcagg ccttcgatgc
    26821 ctgccgggac ctgatcgaca cgctcaaggc gcagaccccg atctggaagc accagagcct
    26881 tcaggacggc tcgaccgagt gggtgggcct tccgtgatcc aaccggagaa cccgaggatg
    26941 cgccccggga gaatctctgc cgccctgctc gtcggctcgt tcctcgtcat cgccgtggcc
    27001 gccgccctca ccttcgtcgg gctgccctac gtcgtcatgt cacccggccc ggcgaccaac
    27061 atcctcggcg agaacgacgg caagcccctg ctcacggtcg acggcgcgca gacctacccc
    27121 acgagcggct ccctggactt caccaccgtg agcgtgaccg gcggcccagg ggtcaaggtc
    27181 accgtgttcg acgtcgtcgg cgcccgcctc tcggggtccg acgaggtgct gcccgtcgag
    27241 caggtgttcc cacccgaggc cagccaggag caggtcgagc aggagggcgc tgccgagatg
    27301 gcgaactcgc agagcgtggc gaccgcgacg gccctgcgtg ccctcgggcg tgacgtcgag
    27361 cagaccgtca ccatcggtga ggtgctcgag gacggcccgt ccacgggtgt gttcgaggcg
    27421 ggtgacgtgc tcgtcagcgt cgacggcgac cccgcgacgt cgtcggatgc cgtgcgcgcc
    27481 gccgtccagc gccacgagcc cggcgagacc tttcccgtcg tcatcgagcg cggcggcagc
    27541 gagcgcaccg tgacggcgcg caccgccgag gcaggcggcc gcaccgtcat cggggtcggg
    27601 ttgcgcatgg actacgacct gccgatcgac gtcacgctga acaccgggcg ggtcggcggc
    27661 ccgtcggccg ggttgatgtt cagcctggcg atctacgacg tgctcacccc cggcgagctc
    27721 accggtggca agaacatcgc ggggaccggc accatgcagg acgacgggag cgtcggaccc
    27781 atcggcggca tccgccagaa gctcgtcggt gccaagcggg ccggggccag ctacttcctc
    27841 gcgcccgccg acaactgcga cgaggtcgtc ggtcacgtgc ccgacgggct cgaggtgctc
    27901 aaggtcgcca ccttcgacga cgggctggcc gcggcgaacg gcatcgccaa gggttctgtc
    27961 gaaggtttgc cgacctgcgg ctgaccccct ccgctgtgtc gggggctcgc tcggctacgg
    28021 cgtgagggtg gcccgcagcg cgtggaccag ccccggggcg atgtcctcgc ccagggcgac
    28081 gcggtcgtcg gtgtcgtggg cccgctgacg cagcaggcac cgggcagcac cgtcacgggt
    28141 cacggcgacg agcaggcgga cgtcggcgcg gtcggggtgc tcggcgatcg cctgcagggc
    28201 tgctgcgtcg tcgctcggaa ggtcacgctc tgcctcgggg ggcacgacga tgcgctccac
    28261 cgccagggcg catcccagca cctgctcggg ccaggcgatc tgggcgagga ggtcctcgag
    28321 tccgtcactt ctcggcaggt cctcctgctc caccgcgctg agggctccgg ccacggcgtc
    28381 gtgtgaggcg agcccctggg ccagctgggg ctcgtgggcg agcaggtcgg cggtcgggac
    28441 gagcgcgaac aggcgcacgg gctggtccca gccgctcctt gccacgtgtc gctcggtgtc
    28501 cagggcggcc agggtgagcg ggtcggtcac gggctggggc gcggcggggg cggggtcggt
    28561 gggggagttc accgcaccat ggtgcctgat ggaacctcca gcaggccggg gtacgttgtc
    28621 ggtccagcgt cggcgcggga caaccctgcg cgatgaggac aagggagagc agtgagtcgc
    28681 tcggagtggt tcgacgggcc ttcgggccct ggtgatggtt cgccgccccc gcagggcgtc
    28741 ccgggtgacg gcggtggggc cggtcggcct gccgccaccg cgacgcgcaa gcggcgcggt
    28801 cccctcgggc cgaccctggc ggtgctggca gttctcgcgc tcgtcggagg gatcgccgcc
    28861 gacgtcatca ccgacgtctg gtggtacgac tccgtcggtt tccgcggcgt cttcgtcaag
    28921 gagatcgcgg tcaaggcggg cctgttcatc ggggccgggc tgctgaccgg tgccgctgtc
    28981 gcgatcagcc tggtggccgc ctaccgaacc cgcccgctct acatcccgat gacgcaggcc
    29041 cagcaggtgc tcgagcagta ccgacaggcc atcgagccgc tgcgccgcct cgccgtgtgg
    29101 tccatcccca tcatcctcgg cgtgctcgcc gggtcggggg ccatgggcgc gtggcgcacg
    29161 ctgctgctct ggatgaaccg cgagccgttc ggcatccaag acccccagtt cggcatggac
    29221 gtgggcttct tcgtcttcac cctcccgtgg ctgcgcttcg tcgtgagctt catcacggtg
    29281 gtgctggtcc tggccttcgt ggcaggtgcg ttcacccact acgtgtacgg aggtctgcaa
    29341 cttcccgggc gcggtccgac cacgcgagcg gcttacgtgc acctcggcat cctcggtgcc
    29401 ctcatcgccc tcacccgggc tgcctcctac tggctcgacc ggtacacctt ggcgacccag
    29461 aagggctcgc tcctcaccgg catcacctac accgacgaca acgcggtgct gcccaccaag
    29521 gcgatcctcg cggtcgccgc gctcatgtgc gccgtcttct tcctcgcggc catctggagc
    29581 cgcagctggc ggctgccgct cacgggtatc gccctgctcg ccgtcacggc agtcgtggcg
    29641 ggtggcgtct accccgcgct gatccagagc ctgcgcgtca agccctccga gaagtccctc
    29701 gaggcgccgt acctcgaacg caacatcacg gcgacccgca cggcattcgg cctcgaccgg
    29761 gtcaagcgca tccagaaccc ggcgccggag gcgaccgagg acaaggacgt gctgcgcacc
    29821 gccgccaacg acatcccggg cgttcggatc atcgacccca acgtcgtcgc ccccacgttc
    29881 cgccagctcg agggccagcg cgactactac gcgtttcccg acaccctcga cgtcgaccgg
    29941 tacaccatca acgaccagac ccgcgacgcc gtcgtcgccg tgcgcgagat caacctcgac
    30001 gggctgcccg acaaccagcg caactggctc aacgaccaca ccgtctacac ccacggctac
    30061 gggttctacg ccgcctacgg caaccagcgc actgccgagg gtgaccccgt cttcttcgag
    30121 ggcggtgggc gtacctcgct cggtgagtac gagccgcgca tctacttcgg tgagctctcc
    30181 ccgacctact cgatcgtcgg tgcccccgag ggcgactcgc ctcgtgagtt cgactacccc
    30241 gccggtggtg acggcgacgt cgccgtcaac aacacctatg agggtgcggg aggggtcgag
    30301 gtcgggtcgc tggtgcgccg cctggcgtac gccgtgaagt accgcgaggc caacttcctg
    30361 ctctccgacg ccgtcaacag cgagtcgcgc atcctcgacc accgcacccc caccgagcgc
    30421 gtcgagcgcg tcgcgccgtg gctgcgcctg gacggcaacg tctacccggc ggtcgtcgag
    30481 ggtcgcatcc agtggatcgt cgacggcttc accacctccg cgagctaccc caactcacga
    30541 ctgatcgagc tcgctgacgc gacgtcggat gccgtgacgc agcgctcgtc ggtcgtcacc
    30601 gtgggtgccg gccaggtcaa ctacgtccgc aacgcggtca aggcgacggt cgacgcccac
    30661 gacgggagcg tgaagctgta cggctgggac accaaggacc cgatgctcaa ggcgtggagc
    30721 aaggcgttcc ccgggtcggt gcggccgctc tcggagatct ccggtgacct catgtcgcac
    30781 atccgctacc cgcaggacct cgtcaaggtg cagcgcgagc tgctcaacga gtaccacgtg
    30841 accaacccgg gtgacttcta ctccaactcc gacaagtggc gcgttccgcg tgacccggcg
    30901 cagaccgccc aggaccagcc ggtggtcttc cagtccatcg ccatgccggg ggaggacgag
    30961 gcagccttct cgatcacgac cccgttcgtc cccgcgagca acaacgaggc cggccgcgag
    31021 atcctgcgtg gtctgctggc cgtcgatgcc gacgcgggct ccgaagccgg caaaccggcg
    31081 gaggactatg ggtccctgcg actgctcgag tacggatcag cgaccccggc cggcccgggt
    31141 caggtgctca accagatcca gaactcgccg gtgcgatcgc agaacgccgc tgagcagctg
    31201 tcgctggcgc agtacatcac caacaactcc ggtggtggca agacgctgac cttcggcaac
    31261 ctgctgacct tcccgctcga gggccggatg ttctacgtcc agccgatcta cgtgcaggcg
    31321 gcagccggtt ctggctcgtt cccgcagaac aagatcaccg tcgccgtcta cggcaccacc
    31381 gtcgcctggg gcgacaccct cgaacaggcc gtgagcgggc tgttcggtca gggcgacggc
    31441 agcgagacgc cgacgacgcc gaccgatccc ggtacgggta cacccacccc gccggacacc
    31501 ggtcagccga ccggatcggc cgcagcgcag ctcacggccg ccctggccga gatgcaggcg
    31561 gcgtacacgg ccgggcagga ggcgttgaag tccggtgact tcacggccta cgaccaggcg
    31621 cagaagcgcc tcgacgcagc gctgaagaag gccgcggcgc tcgctccgca gctcaacccc
    31681 gacggggcgg gagcgacgcc cagcccgtcc ccgtcgccca ccggctgacg cccagccgta
    31741 aggcacatcg gcgctgccct gacactgctc agggcagcgc cgatttggtg cgagcgtgcg
    31801 tgtcacgtac ggttgtcact accgagcacg cctgttcgca ggtgtgcacc cgacgcgggg
    31861 tggagcagct cggtagctcg ccgggctcat aacccggagg tcgcaggttc aaatcctgcc
    31921 cccgctacga agaagggccc ctgaccagca gaagtgctga tcgggggccc ttttgcttcc
    31981 cttcatgtcg tgccgtcggg tccgtggggg tgccgagcgc gcgacttggc acgcggacgg
    32041 catcccaagc ggccccgcct ggcgcgcgga cggcatccga ggtcggtcag cgccggctcg
    32101 ccgagaagcg tgcgatgagc ttgcgcgcct cggcggccca caggaccgtg ctcgccatcg
    32161 cgacgcagac gagccactgc tggcccgtga ggggcacggt ggagaacgcc tcgttgagca
    32221 ccggcaggtg gacgaccgcc acctggagca gcacggacgt ggcgagggca agccacaacc
    32281 agcggttgac gaacaggtgg cggaacgccg agacgctctc cgagcgggcg ttcagggcgt
    32341 tgaagagctg tgcgagcacg agcacggtga acgccgcggt ccgggcggtg tcgatgctct
    32401 cgtcgccctc gatcaggccg ccgggcagat gcaggtcgag ggtgatcagc gccgccgcgg
    32461 ccatgacgag gccgagcccg acgaccccgc gccacatccg ccggtcgatg acccgctcgg
    32521 tcaggggccg tggggggtgc cccatgacgt cctcggtctc gtcgtcgacg cccatcgcca
    32581 gggcgggggc gctgtccgtg agcaggttga tccacaggat ctgggtggcg agcaggggca
    32641 gcacgagtcc gtcgctgccg tgggagaggc cgatgaggcc cccgaggacg acgccgagaa
    32701 agaccgtgag gacctcgccc atgttcgagg acaacaggta gcgcaggaac ttcttgatgt
    32761 tgaggaagat gccgcggccc tcacgcaccg cgcgcacgat ggtggcaaag tcgtcgtcgg
    32821 cgagcaccat gtcggcggcc tccttcgtga cctcggtccc cgtgcgcccc atggcgatcc
    32881 cgatgtcggc cgccttgagc gccggggcgt cgttcacccc gtccccggtc atcgcgacga
    32941 ccttgccgtc cccctggagg gcagcgacga tccgcagctt gtgctgcggc gcgacccgcg
    33001 cgtacaccga ccggtggcgc acggtctccc gcaggccgtc gtcgtcgagg gcctccagct
    33061 cggcaccggt gaccgcgacc ggctcgtgcg gcccgatgcc gaggtcgcgg gcgatgcggg
    33121 cggcggtgcg cgggtggtcc ccggtgatca tgacgacgcg gatgccggcc cgccgcgcct
    33181 cgcggatcgc ggtcgcggcc tcgtcgcggg gcgggtcgat gatccccacc atgccggtgt
    33241 agacgagccc ctcctcgagg gactccacgg cctcgggcgg gtcggtcacg tcgagcgggc
    33301 ggtacgcgac cgccagggtg cggaacgcct cgcccgagag tcgttcgacg tcggcgagga
    33361 tccccgtgcg ccacccgtcg tcgagcggct cgacgtcgtc gccgacctgc acgtgggtgc
    33421 accgctcgag caggacgtcg ggggcaccct tggtcacgac ggcgatccgc ccggcccgct
    33481 cggcgtcggt ggccaagctc gacatgagcc gtcgctgcga ggtgaacggg atctcgccga
    33541 cccgggcgaa gcgcccctcg cgcagcgctc gcgtgccgag cttggcctcg gccacgagga
    33601 acgcggcctc cgtggggtcg ccctgcacaa cgaagccgcc gtccgcctcc tgcgtcagca
    33661 ccgcgtcgtt ggtgaggctg cccccaccca cgaccagtgc ggtctggcgc cacgccgcgc
    33721 tgccgtgctc gagggggatg ccgtcgtgct cgagcgtgcc ctcagggcgg tagccggcac
    33781 ccgtcaccac gacctcgccc gccgcggtga cgacccgccc gatcgtcatc tcgccccggg
    33841 tcagggttcc tgtcttgtcg gtgcagatca ccgacgccga cccgagggtc tcgaccgagg
    33901 agagccgccg caccacggcc tggtgggtcg ccatccgctg caccccgagg gcgagcacga
    33961 cggacaggac cgcgggcagt ccctccggaa cggcggcgac ggcgagcgag acaccgagga
    34021 gcagcgtcgt cacgatgtcc tgcggcgtct cgaccccgag gaagaccacg atcgtggcga
    34081 cgacgaccac ggcgatgacg acgacggcga tgccgagcgt gcgcccgacc cgcgcgatct
    34141 ccacctggag gggggtcggg ttctcgcggg tgtcgtggag caggtgggcg atgcgcccca
    34201 tctcggtcga catccctgtc gcggtgacca cggcccggcc gacgccctgg gtgacggccg
    34261 tgccgttgaa gaccatgtcg agtcggtccc cgagcggcgc cggggccgcc agggtggcgg
    34321 ggtccttgag caccggctcg ctctcgcccg tcagcgaggc ctcggagacg gtgagggccg
    34381 ccgcgctgag caggcgggca tcggcggcca ccgtgtcccc ctccgcgagc acgacgaggt
    34441 cgccgggcac gagctcgcgg gtgggcaccg tgacgcggtc accgtcgcgc accaccgtcg
    34501 ccgaggtcgc gccgaggcgc cgcagggccg cgacggcgtc ctcggcccgg gcctgctgca
    34561 cgtagccgag caccgcgttg agcaccacga tgacggcgat gacgagcgcg tcgacgggca
    34621 ccccgtcggc gccctcgacg gcccacgcca cgagcgacac gacgatggcg gcgaacagca
    34681 ggtacaccag ggggtcgcga aactgctcga gcaccttgcg ccacgtcggg acgggtgccg
    34741 tcgcctccag ctcgttcggc ccgacctgcg cgaggcggcg ctgggcctcc cgcgtggtga
    34801 gcccgccgac cggatcggtg ccgagctcgg cgagcaccgc acccgggtcc agcgtcgacg
    34861 cgtcgcgggg gagtgggggc atcgacacca ttgtctcgca gcgaaccgcc agacctcgtg
    34921 ggggtgacct ccaccacgtc gccgcgacac ggcatacccg gcgtggatgc cgtgttggac
    34981 cgcccgtgag taccccccac cgcgtcgtcg tcatcggttc cggcttcgga ggtctcttcg
    35041 gtacccaggc gctcaagcgc cgggacgacg tcgaggtcac cctgatcgcc cagaccaccc
    35101 accacctctt ccagccgctg ctctaccagg tcgcgaccgg catcctctcc gagggtgaga
    35161 tcgcgccgag cacccgtgag gtgctggccc ggcagaagaa cgcccgggtc gtgctcggca
    35221 cggtgaccag catcgacgtg cacgaccgca cggtcacctc gcacgtgctg gaccgcgagc
    35281 tcgtgacccc ctacgacacc ctgctcgtcg ccgccggggc cggccagtcc tacttcggca
    35341 acgaccactt cgcccgccac gccccgggca tgaagtccat cgacgacgcc ctcgagctgc
    35401 gcggccgcat cttcggggcg ttcgagctgg ccgagctcgc cgagaccccc gccgaggccg
    35461 agcgggcgat gaccttcgtc gtcgtcggcg ccggcccgac cggcgtggag atggccgggc
    35521 agatcgccga gctggcgcac cgcaccctgc gcaaggagtt ccggcgcatc gacccgcgca
    35581 actcgcggat catcctcgtc gacgccgcgc cgaccgtgct gccgagcttc ggcgaacgcc
    35641 tcggtggggc cgccaagcgc cgcttggagg agatgggcgt cgacgtccga cccggcacga
    35701 tggtcaccga cgtcgacgcg gccggcatca cggtgcgtga ggccgacggc tcctccaccc
    35761 gcatcgaggc cctgaccaag gtgtgggcag ccggggtcca ggcgagctcg ctgggccagc
    35821 agctcgccct gcagaccggg gccgagctcg accgggcggg ccgcatcaag gtccttcccg
    35881 acctcaccct ccccgggcac cccgaggtct tcgtcgtcgg cgacatgatc tcgctcgaca
    35941 acctcccggg ggtcgcgcag gtcgcgatcc agggctcgcg cttcgcggcc aagcagatca
    36001 agcggcgcct ggacggcaag gagcccgagc ccgcgttcca gtacttcgac aaggggtcga
    36061 tggcgacgat ctcacggttc agtgcggtcg cctcgatcgg caagttccgc ttctcgggct
    36121 tcttcgcctg gctgctgtgg cttgccatcc acctcgtcta catcatcggg ttcaagcacc
    36181 gcgtcacgac gttgctgcac tggaccgtca gcttcctcgg ccggggtcgg gccgagcgcg
    36241 tggccaccga gcaacaggtc ttcgcccgcc gggccatgga cgcgatgtcg caggacgagc
    36301 agccctcgat cccgaccaac cccgcggaca cgcaggtggc cggagtggct gagatcgagg
    36361 agcagcaccg agcctcggcc tgacccgagc acgaacggcg gtgagccgga tgccgggggt
    36421 gcgccccggc atccggctca ccgccgttcg tgggcacgta gggtgacgcc atgccgctgg
    36481 actacccgat ccaccgccgc acgctgccga acgggctgcg cgtcgtggtg agccccgacc
    36541 acaccgtccc gaacgtcacg gtcaacctgt gggtgggggt ggggtcgcgc cacgagtcgc
    36601 ccggtcgcac cgggttcgcc cacctcttcg agcacctgat gttccagggc tcgaagaacg
    36661 tgccgagcgg tgagcacttc gaggcgctca tggcccaggg cggtcggctg aacgcgacga
    36721 cctggttcga tcgcacgaac tacttcgaga ccgtgccgaa gggggccgtc gagctggcgc
    36781 tgtggctcga ggccgaccgg cacgggcacc tcctcgacgc cgtgacccag gagaacctgg
    36841 acaaccagcg cgacgtggtc aaggaggaga agcgccagcg ctatgacaac cagccctacg
    36901 gcaacgccct gatcgacgtc tacgccgcga ccttcccgca ggggcacccc taccaccacc
    36961 cgaccatcgg gtcgatggag gacctcgacg ccgcgagcct ggccgacgtg cacgcgttct
    37021 tccgggccca ctacgggccg gacaacacgg tgctcacgct gtgcggtgac atcacgcccg
    37081 agcacgggtt cgagctcgtc gagcgccact tcgggccgtt gcccgcgagc gccgaaccgc
    37141 gccgggagaa ccacccgcag ctcgaacccc tggcccagcc ggtgcgggtc gagcggcgcg
    37201 agagcgtgcc caacgaccgg ctgcacatcg cgttccgcct gcccgtcgac gagaccgacg
    37261 agttcctcgc ggctgccgtg gcgctcgacg cgatcggtgg gctcagcacg tcgcggctcg
    37321 tgcgtcgcct cgtgcggcgc gaccagatcg ccctgggcgc ccacgccacc gcgtgggggt
    37381 tcgtcgacgg cgcgtccctg ggcttcgtcg tgctcgacgt cgcccccggc caggacccgg
    37441 atgccgtgga agccgccttc gtcgaggagc tgaccgcctt cctcgcgggt gggccgacgg
    37501 ccgtcgagct cgaggcgtcc ctggctcagt ccgagcggtc gtggctgtcg gcgctggcca
    37561 gccaggagga gcgggccgac gcgatcagcc accacctgct gctccacgac gaccccgagg
    37621 tcgtcaacac ccacctcgac cggctccagg acgtgacggc cgagcaggtg ctcgccgcgg
    37681 cgcgccgctg gctgcagccg cagagccgcg ccgtcgtcgc ctacctgtca gccccggctg
    37741 acgatgaggc tgtcggcgac gttgccgaag actctgccgg cgactcggat gccggtgcga
    37801 acgaggagga ggtcgcgtga gcaccgcccg accgcaggtg accccgcccc agccgtgggc
    37861 cttccccgtc ccgaccgagc acgtgctcgg caacggggtg cgggtgctgc tccacgatct
    37921 cccgggtcag tacgtgttgt cgctgcgggt cgtcgtcccc ctggcggtgt ccgccgaacc
    37981 cgccggccag gagggcatcg cctcgatgac ggcccgcctg ctcgacgagg gcacggcgtc
    38041 gctcgggtcc gacgagttcg ccgagctcat ggagcgccac ggcatggtgc tcggtgccgg
    38101 ggtctccgac ggcggcctgc tcgtcgacgt cgacgtctcc cagcgccacc tcgccaccgc
    38161 cgccggactg ctcacccagg ccctggccgc accggccttc cccgaggacg aggtgcgccg
    38221 catcctgcgc aaccggctcg ccgagatcga gcaggagcgg gcctcctccg cgcaccgggc
    38281 cagcaaggag ttcgcgcgca ccctgtggga cccggccgac cgcgcgagtc ggcccacggg
    38341 tggaaccccc gagtcgatcg gtgcgctcac ccgcgacgac ctcgtcgagt tccacgccac
    38401 ccacgtcggg ccggccgggg ccacggtcgt gctcgccggc gacctcaccg atgtcgacgc
    38461 gctggcagtg ctcgaggcga ccctcggcac gtggtcggca ccgcggcaca ccccgtcggc
    38521 ccgcccggtg cccccgcttc ccgcggcgga tgccgcccgc gtcgtcgtgg tggatcgtcc
    38581 gggctcggtg cagaccgaga tcgtcgtcgg gcgccccggc cccgaccgtt ccacccctca
    38641 cgggtgggcc ccgcaccccg tcctgtcctt cgtgctcgga ggctcgccca gcgcccggat
    38701 cgacgcggtg ctgcgcgagg agaaggggta cacctacggc atccgctcga ccttccgacc
    38761 gcgggtggcc ggcggcacct tcgtgacgtc gggatcggtg cggtccgagg tcaccgggga
    38821 gtccgtcgag atcctgctcg gcatcctcga cggcgcgcgc gacgggttca ccgacgccga
    38881 actgcgcacc ggggtcgact acgtcgccat gaccgcgccc ggccgctacg ccacggcgga
    38941 tgccgtggcc gacgagagcg cgacgctcgc gctcgagggt cttcccggcg acttcacgac
    39001 gcgcaacctg ctcgcggtca acgccctgac gctggccgac ctcgacgccg cgtacggccg
    39061 ggtcgtcacg ggggagtgga ccgtggtcct cgtcggtgac gccgcccagg tggtgccgca
    39121 gctcgacggt cgggtcccgg gctcggtgag cacggttgcc ctctgacgtc gtgccgggcg
    39181 ccgtgcgtgg gccgagctcc tcggatgccg gggcctcacg ctcggtcgtg atggcctcgc
    39241 cgatcgggcg actggagctc atcgaagcgc acggtgcgct caccggcatc cggttcgatg
    39301 cgcctgccga cggcaccgcc ggccacgagc gcggcgggtc gccggtcctc acggcggccc
    39361 accgccagct cagcgagtac ttcgagggcc gtcgccggga gttcgacctg ccccttcgcc
    39421 ccgcgggaac cgagttccag cggcgggtct gggacgtgct cgcgacgctg ccctggggtg
    39481 ccacgacgac ctacggcgag atcgccaacc ggttggggct gccgccgggt gcgtcacggg
    39541 ccgtgggggc ggccaacggg gccaacccga tcccgatcgt gctgccgtgc caccgggtca
    39601 tcggctccga cggcacgctc accgggtacg cgggcggtgt ggagcgcaag gcgatgctgc
    39661 tgcgcctcga gggtgtggcc agcgaggccg accaggcctc cctcttctga cgcacttgtt
    39721 gcacgccagc cacctactgg cggccaggcc aggtggctgg agtgcaatga gtcgtgctca
    39781 gggggtgccg atgcggcgca gcacctcgtc gtgcaggatg ccgttggtcg ccaccgcgtt
    39841 acctccgaag ggaccgtcga ccccggccag tgacgtgaag cgtccaccgg cctcggtgac
    39901 gatcggcacc agggctgcca tgtcgtgcag gccgagctcg ggttcggcgg cgatgtcgac
    39961 gcagccctcg gcgaggagca tgtaggacca gaagtcgccg aacgcgcgct cgcgccagca
    40021 ctcgcgggcc aggtcgagca cctcgcgctc gcggtcgacg tcggcccacg aggtccagct
    40081 cgagcacgag atggaggcgt cctcgatgcg cgagaccgac gagacgtgga tgcgcttggc
    40141 cgaggtcagg ctcttgccag cccaggcgcc cgagccgagg gccgcccacc agcgccggcc
    40201 cagtgcgggc gcggccacga gaccgatgac gggcaccccg tcgtcgacga gggcgatgag
    40261 ggtcgcccac accgggacgc cacgcacgta gttgtgggtg ccgtcgatcg ggtcgacgat
    40321 ccactggcgg gcgctgttgc ccgtggggtc gaactcctcc ccgatgaccg cgtcgcgggg
    40381 cctggtgcgg gccagctgac cgcggatgag ctcctcggcg tcgcggtcgg catcgctgac
    40441 cagggtcagg tccggcttgg cctcgacccg caggtcctcg gcccggaacc gtgccgtcgt
    40501 gacccgctcc acggcatccg cgaggacgtg ggcgagtcga aggtcgtcgt cgtaggcgcg
    40561 cacgaggccc aacctagcgg ccgctgccga agattcttcg gccccgcacc gatctggtag
    40621 cgcttcggtc acaatcccat ctcggcggca cctaatgctt ccgttggggg aatggatggg
    40681 cttctatggt gagcgcacgc attctgcaag agagcgcgca ctggtgggag gcacccgatc
    40741 ggggggctcc catgaacgat ggccatccga caccgggtgg cgacaccact ttcacgtagg
    40801 aggacaccca tgcgaggaac cactcgcacg gtcgcggttg cggcaatgtc tgcagtcgcc
    40861 ctgctcgccg ctggttgcgg tagcggcgac agcgagccgg aggccacgac ggaagccaag
    40921 acgggcggcg agatcaccgt ccgcggctgc aacccggaga acccgctcgt tcccagcaac
    40981 acgaccgaga cctgcggcgg caacgtcatc gacgcggtct tcgccaagct cgtccactac
    41041 gacgccgaga ccgccaagcc cgagatggac gtcgctgagt ctatcgagac caccgacaac
    41101 cagaacttca ccatcaagct caagagcggc tggaagttcc acgacggcac cgacatcaag
    41161 gccaagaact tcgtcgacgc gtggaactac gccgcgtacg gccccaacgc ccagagctcc
    41221 ggctacttca tggagcccat cgagggctac ggcgacctgc agtgcgccgg taccgccaac
    41281 ccggacgacc cgtgtgaggg cttcacgccc aaggccaagg agatgtccgg cctcaaggtc
    41341 gtcgacgacc tgaccttcac ggtcaagacc accctcaagg tcgccaactt cccggtgcgc
    41401 gtcggctact ccgccttctc cccgctgccg gacgcgttct tcgccgaccc ggagggcttc
    41461 ggtgacaagc cgatcggctc cggcccgtac aagctggact cctggaccaa ggagcagtcg
    41521 atcgagctgt ccaagttcgc cgactacgcc ggtgacttcg gtggcaaggt cgacaagatc
    41581 accttcaaga tctacgccga cccggacgcc gcgtacaacg acgtcatcgc cagcaacctc
    41641 gacatcaccg acgagatccc ggcgagcgcc ctgatcgacg acaagtacaa gtcggacctg
    41701 cccgatcgca acgcgcagaa ggagaccggc gtcatccaga cggtcaccgt cgccccggtc
    41761 aaggtcgacc cgaactacgc ggacccgaag atccgtcagg cgatctccat ggcgatcgac
    41821 cgcgagacga tcatcgagaa gatcttcaac aacacccgcg ttcccgccac cggctgggtc
    41881 tccccggtcg tcgacggcta caaggccggc cagtgtggcg agtactgcac cttcgacccc
    41941 gagaaggcca aggccaagct cgccgaggcc ggcggcttca agggtgacaa gatcacgctg
    42001 tcctacaacg ccgacgccgc tcacaaggag tggaccgagg ccacctgcaa cagcatcaag
    42061 cagaccctcg gtgtcgagtg tgtcgccacc ggtgtcgtcg acttcgcgac gttccgcacc
    42121 cagatcggcg agcgcaagat gaagggcatg ttccgcaccg gctggcagat ggactacccg
    42181 tcgatcgaga acttcctcgc cccgatcttc gccacgggct cctcgtccaa cgacggtgac
    42241 tactcgaacg agaagttcga caccctcctc gacgaggctg ctgccgagac cgacgctgcc
    42301 gcgtccaacg ccaagtacca ggaggccgag gccctgctcg cggccgacat gccctccatc
    42361 cccatgtggt acggcaagac caccatgggc tggtcggaga aggtcaccgg tgtgaagatc
    42421 accgccttcg gcacgatcga cttctcgtcg gtgtcgatga agtgaccccg tcgggccgct
    42481 gaatggtccg gcattgcacg ctcgtcggcc tgccagcacc ccgtgttggc aggccgacgc
    42541 gcgtgctcac ccgcactctc ggcgccccgg ggcgccccgg gtgtcggcag ccccgagagt
    42601 gggctaggtt catccccggg tacatccgag acgacgccgt ctgacatcgc gcccgacggc
    42661 tccacacgca aggaggtgac gtgggcacct acatcattcg tcgactgctg cagatgatcc
    42721 cggtgatcct gggagcgacg ttcctcatct tcacgatggt cttcgcactc cccggcgacc
    42781 cactggccgg caagtgcggg gagcgcccgt gcccgcccgc gtacgccgcg gagttccggg
    42841 agcgctacaa cctcaacgac ccgctgcccg tccagtacgc caagtacgtc agcaacctcg
    42901 tccaggggga ccttggggag acgaccaacg gcatcccggt cctcgaccag ttgaagacgc
    42961 gctacccggt caccgcgaag ctcgccctca tggcgatcgc gttcgaggcg atcatcggca
    43021 tcttcgtcgg tgtgctgaca ggcatccggc gaggggggtt cctcgacaac ctggtgctca
    43081 tctcgaccct ggtggtcatc tccatcccgg tcttcgtcat cggtggtgtg agccagtact
    43141 tcctcggggt caagctcggc tggttccccg tgacggtgac ctcctcgaac ccctccttct
    43201 acgagttgat gctgccggcc atggtgctgg gctcactctc cctggcctac gtgtcccgcc
    43261 tgatgcgcac gagcctggtc gagaacctgc gcgccgacta cctgcgcacg gccgtcgcca
    43321 aggggttgac caagcgtcgc gccatcggtc tgcacgccct gcgcaactcg atgatccccg
    43381 tcatcacgtt catcggtttc gacttcggtg ccctgctcgg tggcgcgatc gtcaccgagg
    43441 ccatcttcaa catccccggc atcggcggat acctcttccg ctccatccgg gcccgtgacg
    43501 gtgcctccgt cgtcggcacg gtgaccgtgc tcgtcatcgt ctacctgttc gtcaacctgc
    43561 tcgtggacct gctctacggc ctgctcgacc cgaggatcag ccatgactga agctgctgcc
    43621 gtcgtctccg agaccgtcgc tcccaacggg tcctcggacg agaaccgctc gctcgcatcg
    43681 gacgcctggc gatccctgcg caagaacccc atcttctggg cctcggctgt gctcatctcg
    43741 atcttcgtgc tcatggcgat cttcccctcg ctgttcacga gcaccgaccc ccgcgaggcg
    43801 gtgctctcgg agtcgcgctc gcgccctggt gacggcacct ggttcggccg ggacatccag
    43861 ggctacgaca tctacgcccg gtgcatctac ggggctcgag cctcgatcct cgtcggggtc
    43921 ctgaccacga tcctcaccgt cagcatcggc ggcttcatcg gagtcatgtc cggctacctc
    43981 ggcggtgccg cggatgccgt gatgtcgcgg gtcggggacg tcttcttcgc cattcccctg
    44041 ctgctcggcg cgatcatctt cctggtctcg ctgccggagt tcttcagctc caacgcgttc
    44101 ctcggggcgc tcaaggtgtc cctggcgctg gccgtgctcg gctggccgag catggctcga
    44161 ctcatgcgca gcagcgtcat ccaggtcaag cccaacgact acgtgcaggc ggcccgggcc
    44221 ctgggtgcca gcccctcacg gatcgtgcgc tcgcacatcc tgccaaactc ggtggcgccg
    44281 ctcatcgtcg tggcgacgat ctccctgggt gggtacatca ctgccgaggc gaccctgtcg
    44341 ttcctcggca tcggcctgca ggcgcctgcc gtctcatggg gcatcgacat cagtgccgcg
    44401 ctggtcggtc tgcgcaccac cccgcacatg ctgttcttcc ccagtctctt cctgtcgctg
    44461 gcggtgctcg gcttcatcct cctcggggat gccgtgcgcg acgccctcga cccgaagaac
    44521 cgctgagccg acggaggcct catgaccacc attcaggaca cccagacagc cggcagcgag
    44581 taccagccgg tcgatggtct gctgctcgag gtcgaagacc tcttcgtcga gttccacacc
    44641 cccgacgggg tcgcgacggc gatcaacggc gtcagcttcg agctgcgcca gggcgagtcg
    44701 ctcgcgatcc tcggtgagtc cgggtccggc aagtcggtga ccgcccaggc gatcatggga
    44761 atcctcgaca tgcccccggc cgtcatcccg agcggccaca tccgctactg cggtcaggat
    44821 ctgctgacga tgccggagga gcagcgccgc aagacgcgtg gcccggagat ctcgatgatc
    44881 ttccaggacg cgctgagctc gctgaacccc gtcttcccgg tggggtggca gatcgccgag
    44941 atgttccggg tgcaccgtgg cctcaacaag agcgacgccc tcgagaaggc catcggcctc
    45001 atggagcggg tctcgatccc cgcggcccgc gagcgggtca aggcctaccc gcaccagttc
    45061 tccgggggca tgcgccagcg catcatgatc gcgatggcca tcgcgctcga cccggcggtc
    45121 ctcatcgccg acgagccgac caccgccctg gacgtcacgg tgcaggccca gatcatggcg
    45181 ttgctccagg agctgcagga agagcgccac atgggcctca tcctcatcac ccatgacctc
    45241 ggcgtcgtcg ccgacgtcgc cgaccggatc gccgtcatgt acaccggtcg gctcgtggag
    45301 accgccgagg tgcacgagct ctacgcccga ccggcgcacc cgtacaccaa gggcctgctc
    45361 gactcgatcc cgcgcctgga ccagaagggg cagacgctgg ccgccatcgg tggcctgccg
    45421 ccgaacctgc tgcgcatccc accgggctgt ccgttcaacc cgcgctgcca catggctcgt
    45481 gacgtctgcc gcacggatct tcccccgctg cacgaggtca cgcccggccg gttctcggcc
    45541 tgtcactacg ccgaggaggt catcaatgcc tgaggtcatt ctcagcgctc aggatctggt
    45601 gaagcactac ccgatcaaga agggcatcct caggcgcacc gtcgggcacg tcaaggccgt
    45661 cgacggcgtg agcttcgacc tgcaccaggg ggagaccctg ggcatcgtcg gcgagtccgg
    45721 gtgtggcaag tccaccctgg gccgcctgct catgcgcctc gaggagccga cgtcgggcaa
    45781 ggtgatcttc ggcggtgacg actggagcgg tgcaagcggg cgtgacctgc ggcggatgcg
    45841 gcgcgacatc cagatcgtct tccaggaccc gtacacctcg ctgaaccctc ggatgacggt
    45901 cggtgacatc gtcggagaac ccttcgagat ccacagcgac gtcgtgccca agggtggtcg
    45961 acgcaagcgg gtgcaggaac tgctcgacct cgtgggcctg aaccccgagc acatcaaccg
    46021 gtacccgcac cagttctcgg gtggccagcg tcagcgcatc ggcatcgccc gcggtatcgc
    46081 gctcaatccc aaggtgctcg tgtgtgacga gccggtctcg gcgctcgacg tctcggtgca
    46141 ggcgcaggtc gtcaacctca tggaaaagct ccagaacgag ctcgggctgg cctacgtgtt
    46201 catcgcccac gacctgtcgg tggtgcggca catctccgac cgggttggcg tcatgtacct
    46261 cggccggatg gctgagctcg gcgaggagga cgaggtctac acccgtccga cccacccgta
    46321 cacgcaggcg ctgctctccg cggtgccggt gccggacccg actctgcgtg gcaagcgcga
    46381 gcagatcgtg ctcgtcggcg acgtcccgtc gccggcgaac ccgccgtccg gatgccgttt
    46441 ccacacgcgc tgctggaagg cccaggaccg ctgcaagacc gaggaacccc agctcgagca
    46501 gcgcccggac ggggtcgggg cgcaccgttc ggcgtgtcac ttcgccgagc cgcgggtcat
    46561 cgtcgagacc gtcgacgtct ctggcgtcgt gcccgacgag cggttcgcgt ccactgacgt
    46621 gcgcgacacc gtcgtctccg acgacgcacc ggcagacctc cagcggatga tcgaccggtc
    46681 cgaggcgtcc tccggcgacc aggcgtacgc gccgcaggac gtcacccgcc tcatcgacga
    46741 gcagcgcggg gaccaccggc cccccacggt ctgacccccg ctctgcgact cattgcactg
    46801 cagccacccg gatccgggtg gctgcagtgc aatcagtcgg tggtgggggc tgagcgggcc
    46861 cgcagcaacc ggcgcaggga gtccagtcgc gcggcaccgg ccggcccggc atccccgctg
    46921 gcgacccagg cgtcgagacc gcactcgggc tcgtcgtggg tgcaccccct cgggcaggtg
    46981 gccgtgcccg gctcgaggtc ggcgaagtgc tgcacgatgc ggctgaggtc gacgtgggcc
    47041 aacccgaagg agcgcacccc gggggtgtcg atgacccacc cgtcgtcgtc cggcagcgcg
    47101 agcgcgacgg ctgaggagga ggtgtgccga ccccgcccgg tgacgtcgtt gaccacacct
    47161 gtcgcacgca gggcctgcgg gacgagcgcg ttgacgagcg tggacttgcc gactccggag
    47221 tgaccgacga gcacgctgac ccgccccgac aacgcctcac gcagcgtgtc gaggccgtcg
    47281 gtgcgtgtgt cgcggcgggc ggtgacgacg tgtcggaccg acagcggcgc gtactgctcc
    47341 aggaaggggc ccggatcggt gaggtcggcc ttggtgagga cgagcagcgg ctccatgccg
    47401 gcgtcgtagg cggcgaccag gcagcggtcg atgaggcggg gtcgcggctc cgggttggcc
    47461 aacgcggaga cgacgacgag ctggtcggcg ttggcgacga gcacccgctc gaccgggtcg
    47521 gtgtcgtcgg ccgtacggcg caggacggtc tcgcggggct ggacccgcac gatcctggca
    47581 aggctgccat cagccccgct cgtgtcgccg acgacgtcga cgcggtcgcc gacgacgatg
    47641 cccttgcggc cgagttcacg cgcgcgcatg gccatgacga cgtgccggtc gccgccgtct
    47701 ccgacgagcg tggtgtagcg gccacggtcg acggtgatga cctgaccggg cacggcgtcc
    47761 tcgtgggccg ggcggtcctt gctgcggggt cgagagcccc tgcggttggg gcgaatccgt
    47821 acgtcggact cgtcgtagtc ccgcgaggag cgcgccatca ccgctccgcc gtggcccgct
    47881 cggacagcat gcgctcccac agcgtcgtga actcgggcat cgtcttcgcg acggtgccca
    47941 cgtcctcgac gacgacgccc ggcacgacga gcccgagcac ggccccagcc atcaccatgc
    48001 ggtggtcggc gtaggtgtgg aaaaggcctg cgcgcagcgg cgccggggtg atcttcagcc
    48061 cgtcctcggt ctcgacgacg tgtgccccga gcgcagtcag ctcggtgcgc agggcggcaa
    48121 gccggtcggt ctcatggcct cggatgtgcg cgacccctcg gatgacgctc ggtgcctcgg
    48181 cgagcgcgca gagcgctgcc accaccgggg tcagctcgct cgagtcgtgc aggtcgacgt
    48241 cgatgccggc gatgccgtcg ccggccgtga cggtcaggcc gccccggtcg agcacgacct
    48301 cggcccccat catgtcgagg acgtcacgca ggaagtcgcc tccctgggtc gtgtactgcg
    48361 gccagccggg cacgtggacc cggccgccgg tgacgagcgc tgccgcgagg aactgggccg
    48421 cgttcgacag gtcgggctcg acctggacgt cgagcgcatt gatctcactg ggctcgaccc
    48481 gccaggtgtg cacctcgccg tcgtcgacca cgaccccggc atcgcgcagc gtctcgaccg
    48541 tcatctcgat gtgcggcagg ctcggcacgg ccccaccgtc gtggtgcacg gtgacgccct
    48601 cgtcgaagcg cgccgcagac agcagcagcc cggagacgaa ctgcgaggat gccgaggcgt
    48661 ccagcgtcac ggagccgccg ggcacccggc cccgcccgtt cacggtgaac ggcaggctgc
    48721 cggagccgcc gtcgtcgaga tcgacgccga gggctcgcag cgcgccgatc accggagcca
    48781 tcggccgctg ccgggcgtgg gggtcaccgt cgaagcggac cggcccggac gcgagtccgg
    48841 cgagaggggg gacgaagcgc atgacgttgc cggcgaggcc gcagtcaacc tggccacctc
    48901 cgcgcaccgg gccggggctg acgatccagt cgccgccggg gacgtcctcg atctcggcgc
    48961 cgagcgtgcg cagcgccccg gccatgagca gcgtgtcgcg ggaccgcaac ggcgcccgca
    49021 ggcgggaggt gtcattcgcg agggcagcca gcacgaggta gcggttggtg agggacttgc
    49081 tgccggggag gacgacgtgt gcgtcaaccg gcccgtgggc gctcggcgcg gcccaatcag
    49141 ctgagggcat gctgggcctg gaccgccttc agcttcgtct cccgagcggc ggcacgtgcc
    49201 gcgtgacggg cttcgcggcg cgtggtgcgc accgcccggt gggcgctgtc gctcgcgagg
    49261 tgggcccgat aggccagccc gggcttgccc tcggtgtcga cggccgcgag gagcaggccg
    49321 ccgaggaggc cgaggttctt caggaactgc accttctggc gctcgcgcac ctcgggatcc
    49381 tgttccttcc agaacgcgtg tgccgcaacg gtcgtcggca ccagggtgac ggcaaggaca
    49441 ctcgaggcca cgcgggggag gtgcccggtc gcgagcagcg cgcctgcgcc gaccatggcc
    49501 atcccgttgg cgcggatgag cagttcgggg tcatcgggga gcccgacgcg ggggccgacc
    49561 ttcgccagca gtgggccggc ggtcttcgcc ttcgccgtgg ggtgcttgaa ctggtccagg
    49621 ccgccggcga tgaagatcgc ggcgagcagg ggtctggcga ctcgacgaac gagtgtcatg
    49681 gggtctccgg tcggttccgt cgattgagga cgtgctcttc ctacggtagt tgctcccgca
    49741 gcccctgcgt agattgagcg tatgtgtggt cgttatgccg cgacagccaa tccggacgaa
    49801 ctcgtcctcg agttcgaggt cgacgacgac cgcaccgggg agcccacgcg cagcatcctc
    49861 gtctccccgc aggagccgcc cccggggcga cccgaccaca acgtcgcccc gaccaagcag
    49921 gcccccgtgg tgctggaacg tccaccgcgc gatgaccggg acgcccctgc ggttcgccag
    49981 ctgaggttgc tcacgtgggg tctggtcccc agctgggcca aggacactcg gggcggtgcc
    50041 cggatgatca acgcccgggt cgagtcggtg gccgagaagc cgtcgttcgc caaggccctg
    50101 gcgacgaggc gcgcactggt cccggcgcgc gggtggtacg agtggcaggt ctcgccgacc
    50161 gccctggacg ccaagggcaa accccgcaag cagccgttct tcacctcgcg ggtcgacggc
    50221 gccagcgtcg ccatggcggg gctctacgag ttctggcgcg acccctcggt ggccgacccc
    50281 gacgatccga tggcgtggct caccaccttc acggtgatca ccgggcccgc agagccgggg
    50341 ctcgaccgga tccacgaccg agcccccatc gtcctcgaac cacgtcactg ggacgcctgg
    50401 ctcgatcccg caaccggggc tgccgaggtg ctgggactgc tcgagcccta cccaccgggc
    50461 cgcttcaccg cctggccggt gtcacccgcc gtgagcagca accgcgcgaa cggcccacag
    50521 ctgctcgacc ccctgcccga ggaccagctc gtcggcgtgg tcgatccggt gacgggtgag
    50581 gtcatcgggg ccccgaggtg acccgttccg agacgaccgc gctcgtggag acccccgagg
    50641 ggccggcgcg aaccaccgtg acccagcccg acggcatccc ggtcggtctg ctcgtcctcg
    50701 ggcacggtgc cggcggactg cgctggaccg acgacgtcac cgccagcagg gatgcagccg
    50761 tggatgccgg gtggacggtc gcgctcgtcg accaaccgtg gcgcgtggcc gggcggcgga
    50821 tcggtggcca gccggcatcc ctggaccggg cctggctcgc ggtgctcccg acactccacg
    50881 cccaccgtcc gaacgggggg ccgctcgtcg tcggtgggcg cagcgcaggc gcccgggtcg
    50941 cctgccgcac ggctgccgcg gtcggggcga acgcggtgct ggcgctgtcg ttccccctgc
    51001 acccgcccgg tcgtcccgag cgatcgcggg gtgaggaact gcgcgaaccg ggccggcacg
    51061 gcatcaccgt tcacgtgatc caggggctga gtgacgcgtt cggaaccccc gaggaggtgc
    51121 gcgaggtcct cacgggcgca gccgttcacg aggttgcggg aacccactcc ctggagcgct
    51181 ccgcggccct cgtggcgacg gcggtgacct ccgccctctc gcggtgctga cccctgaatc
    51241 tcggacgtcg gggggaatgc tccacggaga ggtggtgttg accgacctgt tccgaagttc
    51301 gccgtccgaa aggttcgaca ccgtgctcgt cctcgacacc acggccccgc gccgggcccg
    51361 gataggctgg gaagtgatga ccactccaac cacgcccgtc gacgagagcc ctgccgagcc
    51421 catcgacgtc gccaccgagt ccacggcaga ccgtcaggcc cgcttcgagc gtgaggccct
    51481 gccgctcctc gaccagctgt acgccgcagc gatgcgcacc acccgcaacc ctgccgacgc
    51541 cgaggacctg ctccaggaga cctacgccaa ggcgtatgcc gccttccacc agtaccagcc
    51601 ggggaccaac ctcaaggcgt ggatgtaccg catcctc
//
LOCUS       DAOSOC010000003        43779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_914_length_43779_cov_9.928529, whole genome shotgun sequence.
ACCESSION   DAOSOC010000003 DAOSOC010000000
VERSION     DAOSOC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 43779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43779
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_914_length_43779_cov_9.928529"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PL014_00510"
     CDS             <1..290
                     /locus_tag="PL014_00510"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOT55599.1"
                     /translation="IPGKMVKGMGGAMDLVHGAKKVIVLMEHVAKDGSHKIVSECSLP
                     LTGKAVVQRIITDLAVLDVTQDGLVLRELAPDVTEEQVREATGAPFSVALD"
     gene            complement(324..1598)
                     /locus_tag="PL014_00515"
     CDS             complement(324..1598)
                     /locus_tag="PL014_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT55600.1"
                     /translation="MRALRVTVAFAVAAGAVFTGSASSAAGVGASAPSAATASASTTY
                     PRPAGTSFTLSGRGYGHGRGMSQYGAYGAALKGLSRNEMLSFYYPGTTRSTSIGNPTM
                     RVRLTALGSTTTQLVTTPGLVVSDGTKTGALAVRNSDGTPRERWRLVPEGTGLTLQWL
                     EKGSWRSTSSWRARTVPLSFTDASLGAVRVVLPDGTQRDYRRTVRGIRWGAGMMTLSV
                     VSMDYYLQSVVPSEMPASWPTAALEAQVVAARTYAAHQRANQAPGTPYDTCDSTSCQV
                     YRGMAGYTTAGTQVPHENARSTAAVASTAGLGLFANGSPALTEFGSSNGGRTVKTALP
                     YQVSLADPYDAVPSGSTSRWTKTLPISAIEKAYPTIGKLKALRIDKRDGIDAWGGRII
                     TMTVIGSAGSKTVKGTAFSAALGLRSTWWTVS"
     gene            1769..3232
                     /locus_tag="PL014_00520"
     CDS             1769..3232
                     /locus_tag="PL014_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55601.1"
                     /translation="MASTGDEPPGVGGSLRSRAALSTIATAVQGLGRLLYSVLIGHLG
                     SRELLGQTNTSLSLSVLTSQLWAAPAATAGTRFVAMKATLDDPEGAATVARHIATRTA
                     LISMVLPTAVALAGSAWLGFGAAHTIGTVVLAFTYSMYVTLRGVQYGALRFRHVAVWD
                     AIAAATALLTVTVVLALDLTVVALLPLTLGYALFAVASWPARVAARVEAPLRREIDRF
                     VFFGAVSGLASGGLLQLSQLVAHSVAGPAEAGDFAAALTLATPASMLSIALSTVLVPP
                     LVAAAGRGDRAAVHAQSDAIARRLTAIFVGLFGVLVIVSPLGIALVWGEEYRAAAGIL
                     PVLLLAVMLTSIALGAATTLQSTRARGPRAVAYLNLGGLVVSLAVWPVLAPRLGTTGV
                     ALGYLVGAGAASLGALATVWWVERHRWLDLAARLGGGIALVVVLAALARSVDGVGGAV
                     AQVGAALLFGAVWLALNRREVAALWATVRGSGGTPTA"
     gene            3242..4111
                     /locus_tag="PL014_00525"
     CDS             3242..4111
                     /locus_tag="PL014_00525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT55602.1"
                     /translation="MPPTLTVLMTTYHGTSVEELERSIASITGQTRTPDEFLVVVDGP
                     VPQPLDDALAAAAAVHPGIRVERLPTNVGSGLASARGLELASGEFVARHDSDDVSLPH
                     RLERQMDAVRDHHLDVVGSSMLEFEGSPDHVVGLRRNPRTHEQIAARMRLNNPINNPT
                     AVFRRELALEVGGYADLRYMQDYDLFARMLAAGARAENLDEPLVLFNAGDGMISRRGG
                     WRMLPYEWQVQRRLRDTGTIGNVLLARNMVVRGTFRVIPPTLLKRVYAVLFRRGSGTP
                     PRDTGASGATGTP"
     gene            4108..4923
                     /locus_tag="PL014_00530"
     CDS             4108..4923
                     /locus_tag="PL014_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55603.1"
                     /translation="MSGGVGRGPVLTWVIVSYGGVDDAVRLVDSLGAGSPVDIVLCAN
                     RPGDTDEATAAFAGDRRVRVLSFEDNPGYLPALQRALPHVDTTRPVVLSNCDLLADPG
                     VVDDLIEQVEAFPDAGWLAPSIIGSLGQDQNPNLVHPPSARWLRALAAIHRFPPLTDL
                     LLLRKEGHSSRTVAGVDAGSTVFAGHGSCIVLTPAFFAAGGSCDYPFALFGEELWFGR
                     ECARLGLTVRYVPSAVLRHAEHAATGRRRRGHVARVKYEGLRWWAREARAQRW"
     gene            4979..5665
                     /locus_tag="PL014_00535"
     CDS             4979..5665
                     /locus_tag="PL014_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT55604.1"
                     /translation="MSSAHRTNDDVWLVVPLFNEETVIADVVRDARKTFPNIVVVDDG
                     SSDHGADAAASAGAMVVRHPVNLGQGAALQTGFEYALGVPTMRWVVTFDSDGQHQVSD
                     VLAMLDKARTEDLRVVFGSRFLDSRTTPSFMKKIVLRLAVGYTNVTTGTRLTDAHNGL
                     RLLHREVVERLDITQNRMAHASEIVAQIGDMKVPYGESPVHILYTDYSKSKGQSLWNS
                     VNILVELMFR"
     gene            5662..6027
                     /locus_tag="PL014_00540"
     CDS             5662..6027
                     /locus_tag="PL014_00540"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HOT55605.1"
                     /translation="MSTVVIIQVVLITLVVLVVARLFRSRGARAQAIRRIGLVVFAAF
                     AAVSILFPDMWTKIAQKVGVGRGADLVLYALVVAFLSFTVTTYLRFRDLETNYTRLAR
                     RIALSDAPAPPNHPDDPRA"
     gene            6024..7994
                     /locus_tag="PL014_00545"
     CDS             6024..7994
                     /locus_tag="PL014_00545"
                     /inference="COORDINATES: protein motif:HMM:NF039646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HOT55606.1"
                     /translation="MTLGPLLERAGRSLVTAALVLFAVWTVVYQVALAVGLPSTPTLV
                     VALLIGAGTLVLLSHTTTSNDSVVAPVPGAAVSLAVLGVTVVATALGAYGMRGPALAL
                     AVLTAVTALAVTARRGAPPAGQAAATGGPASDDASAADPRTWTVGWVGALVSAALASI
                     IARPDGDDAYFVNLSAWVAEQGRFPLRDTMISPDVYPALGAHSPPVHSIEGLIGALAR
                     VLHIEAGTATYVLVPPLATALAVLVLTRIVTAARIPAGPVALLAALGFLWTTGGSGYS
                     FGNFFAVRMWQGKSMLVSIVIPLVILFGVELIRRGSARAHLLFGASLIAAVGMSNTAV
                     FLVPVLVGGLVLAALALREIRGAVRLSLWVVYPIIAGLATLVFATPSPTKAQLDVEGF
                     VLAASRAGDPLMTVPGRHGIYVVSALALGLGILGLREVGLRTAAIGSLAAAGVMLLPP
                     VRGILEGIGLTSVVWRMWWVVPIPLLVAGLVGAAALFLPRGWPRAVGGLLAMLLVVLV
                     PLVGGRWVGSEGNNARFVSPMAWKVPRGALSEARFIEQVSQPGDIALAPWDTSRVLAA
                     LTVDVQPVSARRAYLPTYAGTPEAQAGSREELQEFADERTPGTDTIGDDLDAVGVDTA
                     CVGTSRGRAIDLLEANGFEVVGVINNLTCLRR"
     gene            complement(8020..8769)
                     /locus_tag="PL014_00550"
     CDS             complement(8020..8769)
                     /locus_tag="PL014_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55607.1"
                     /translation="MSVVITAKDVGVEFYTSRRRKMQVRDLLFHGRATTSTDTFWALK
                     DISFEITRGEAVGLVGGNGSGKSTLLKTIAGVLLPDRGTVEVNGGVAPLIELTGGFRG
                     ELTCRDNLWVQAGLHGLSKAQIEERFEDIVDFAGPQVRAALDRPYRHLSSGMQVRLGF
                     AVISSLDEPIFLVDEVLAVGDKAFREKCYTRMESLLSEGRTLFLVSHSERDLKRFGTR
                     GLYLRQGQLVLDGPMDEVLDRYNEDQGTQQR"
     gene            complement(8774..9568)
                     /locus_tag="PL014_00555"
     CDS             complement(8774..9568)
                     /locus_tag="PL014_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55608.1"
                     /translation="MIKRITAVWQRREILGTLVQRDLRVRYSRSVLGYVWTVLDPLLM
                     ASVYFVVFTFIFKAGRVADTPYFLYLLSGLLAWQWFTGAVTDTTKSLIQEARLVRSTN
                     LPREVWVIRCVIAKGVEYVLSLPILVGFVIYYLVKGRVDLDWELLLFPLGVGLQFLLL
                     VGLGLILAPITVLATDMQRVVRIFLRFFFYLSPVLYGLHSVPDQLRAVLVLNPLNGIL
                     SLYRGGLFERSVSWVDVGVSVAMSLLFIVVGFWTFSRLERSVLKEI"
     gene            complement(9669..10883)
                     /locus_tag="PL014_00560"
     CDS             complement(9669..10883)
                     /locus_tag="PL014_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55609.1"
                     /translation="MLTKSYSLLNFLNRSHSPAFRYGIGGAAYAGTLAVTGRPSIARL
                     VRTQFVEDETKYAFRLAGWVLRRGWPQVETVTDDFFDFTLGATPADLESLEAFIAHRR
                     DALPEHLWRADDLFVRAARIHAQLDAGARDIGALREDFRAHADDLLALISSAQGGPSA
                     PASDREGDFSVDDARTALVDLADALPVQQWRWYVISGTFLGIVREGGFLAHDYDIDVG
                     VTFDPQHPEVLDDLVAALRRSPRYVVKKLDDAQTVVEVEPGRFTVERAPALVKLIHEN
                     GINVDVFVHHLEGDRLWHGSSIHRWENSAFDLADYTLAGVPVLGPADADRYLTENYGD
                     WRTPVTQFNCTTGTPNLVITRSFRSVALFLTRLAHFAHGDPGEYARLRATLIQDGMLR
                     DVDGILVLERDI"
     gene            complement(10883..14434)
                     /locus_tag="PL014_00565"
     CDS             complement(10883..14434)
                     /locus_tag="PL014_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOT55610.1"
                     /translation="MVVPIYNVEDYLVSCLESLADQTFSDLEVLLVDDGSPDRSGEIA
                     DEFAQGRPGWRVLHVENGGLGRARNIGLDAAGTEFVTFVDSDDVVPRDAYELMMHAIE
                     GSGSDIVSGGVLRYDGARTRPSGLHRRAVPETRIGTHVRAMPSLLYDTTAWNKIFRRE
                     FLIEHGLRFPEGVLYEDIPLTVPAHFLARSVDLIEEPVYLWRERQTAEQSITQRRAES
                     RNLIDRMAAVSSVNSFLERTGEVDGKRLHDEKVLTLDMPLFLDVLHEGDEEFAETLVR
                     VFREYLADVDPAVIAKLPPRRRLSYHLIRENRTAELVEAHQLQVRGPRPEVVRRGVRL
                     YVQLPYFGDSSVGVPDSVYEVTRSQRLVTGIRDVRWKGSSLEIDGHGYIDGVPDIGPG
                     TTVHRMQLRVVGSKAERRSVRARRVRRVDITGRARNRAVNYDGSGFLARVPAADLVLP
                     DGKDAVEYELVAQVAAPAARRGSVVGSPEYSRALHPPRGWAPDGVLVVPGYRGKKMRI
                     AARRAPVVVRDAVVEGEEVVLTLRAAPGTQLPDSYVHLRRLDASEGVTVRVDREGATG
                     TARIPAESLHVTSRSLAERHWRLWLAVAAADDPQDASAVVDELVTVASSTEREAPRSL
                     RINGRHVGPLDVDPEAGAGRARLHGRLLQLRERGVTGAVLVDGPLRPLLTDFAFTPDG
                     LELHGDTEDVDLDQLVLLSRSDRLEVPVEVLGGTWRALVPATGSPGQPCLRWLRPGRW
                     SVLAGVHGDDEATLGVHVSAGAEAHLSDTGGEKVVVVLRTSPSHELGLVVDGGGAWRD
                     RGAFNQKIARRVHYRLARRLPLTDTVFFEAWKGRQYSDNPRAIFEELVRRGDGRRQVW
                     AVEHHGVEVPDGVETVVVGSREYFHTLGRARWVVSNDSMPKHYVKRPGTVYGQTWHGT
                     PLKRIGFDIENLQMANKNYLTQFAKEVATWDALVSPNAYSTQIFRRAFRFDGRVLETG
                     YPRNDVFHRPQERAARTAQAAARLGIEPGKRVILYAPTWRDNQYDRSGRYQFAMKLDL
                     ERMHREFADDAVLLIRGHQLVANSVDTSMFGDFARNVSHYPDISDLYLVADVLVTDYS
                     SVMFDFVNTGRPMLFFTHDLEAYRDDLRGFYIDFEAEAPGPLLTHTNDVIDALGDLDR
                     VVAEHAGRYEAFRQKFASLEDGDAAARFVDQLLTDGS"
     gene            complement(14568..16088)
                     /locus_tag="PL014_00570"
     CDS             complement(14568..16088)
                     /locus_tag="PL014_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT55611.1"
                     /translation="MGSQSGSAVLDRTATEAPIRVDTRTTGDPRRDRRRAYALLGLTV
                     VLPGSAQLAVGNPTLGRFALRGWFGVWAGAALLGLLAVVNRTAALTVITHRWFLAVLQ
                     VVLFAMAALWLGLLVDAWRIGRPDRLARTDRRRLLVALVVLLVILPGGTAYAGVNVGA
                     ARTAMTSVFGAGDAAGAVDGRFNILLLGGDSGRGREGLRPDSIQLASVDAETGRAVLF
                     GFSRETEHITFRPGSVMAGLMPQGWTCGDECLLNGLYTWGRDHADEFPAGTKDPGLLA
                     TTEAVEALSGLDIQYHVLVDLKGFRSLVDAVGGLDITVQRRTPIGSQANIKGWIEAGE
                     RHLDGHDALWYARSRANSTNYERMARQRCVVTAMIRQLDPQTVVLNFGDIANATKGVF
                     QTDIPQGALATLGDLAVKTKQQKITSVNFVPPLIKPWDYDPAVVHAAVDKAIARSEQA
                     DEKAAEERAAGPEASTASGGGAKAQATEPKKQAVMDRPGSDPDADTSDLASVCSAG"
     gene            complement(16297..16554)
                     /locus_tag="PL014_00575"
     CDS             complement(16297..16554)
                     /locus_tag="PL014_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55612.1"
                     /translation="MKTTQAATAARTSRDRRRARPDRGGRVERGVRGPRGGVGGVGRP
                     GETGRPSSSYAVWPGFGAVAARRGFAVEVGAGWLARRVSTT"
     gene            16556..17473
                     /locus_tag="PL014_00580"
     CDS             16556..17473
                     /locus_tag="PL014_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT55613.1"
                     /translation="MVTPIHAWSQVAREDTTPTTQRPADASGSTYLLVGSDSREGLTA
                     AERKAIGVGGDAEGRRTDSIILVHTPSGSGKPVLISIPRDSFLPIPGHKDNKVNAAFA
                     IGGPSLLVATLEEATGLPIDGYVEIGLGGFAGVVDSLGGVEVCVKNDIKDAKAHIDLK
                     AGCQELDGKNALGYVRARYSDPRGDLGRAERQRQFLGAVMKQAATPSTVLLPWRWWGF
                     THTATSSVVVGEDTSLLDSYRILSTMRKVSSDQALSLVVPLSSTNTTTRAGSSVIWDD
                     ERAGALFTMLREGTPIDSPPEGTDGRPRG"
     gene            complement(17510..18676)
                     /locus_tag="PL014_00585"
     CDS             complement(17510..18676)
                     /locus_tag="PL014_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT55614.1"
                     /translation="MSGNPDFDTYRLSEDHEAIREAVRAVAQDKIAPWAAAVDEEARY
                     PQEAHDALVASDFFAPHVGEEYGGVGADALATCIVIEEVARADASASLIPAVNKLGSM
                     PVILAGSEDLKTRYLTPLAAGETTFSYGLSEREAGSDTAAMRCKAVRDGDDWVLTGQK
                     SWITNAGVSQFYTVLAVTDPDGPRGNNITAFVVEKDDPGFTFGEKERKLGIKGSPTRE
                     LHFDRTRIPGDRVVGTVGGGLKLALATLDHTRVTIGAQAVGIAQGALDQAVAYVRERR
                     QFGKAVAEFQGVQFMLADMAMELEAARQMVYVAAAKSERGDADLPFFGAAAKCFASDV
                     AMKVTTDAVQLLGGAGYTRDFPLERMMRDAKITQIYEGTNQVQRIVMARQLLKG"
     gene            complement(18744..19805)
                     /locus_tag="PL014_00590"
     CDS             complement(18744..19805)
                     /locus_tag="PL014_00590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT55615.1"
                     /translation="MKFGLFIPQGWRQDLVGIDPAQHWGVMAGLARRVDGNPDWESIW
                     VYDHVHTVPRPIEETTHEAWSLMAAFAGVTGRVRLGQMCTCMGYRNPAYLAKVAATVD
                     VISEGRLEMGIGAGWYEHEWRAYGYGFPSAGERLQALREGVEIMQQMWTTGYGNYAGE
                     HFTVDGAMCFPRPLQGDSAPGSPRNGIPVWIAGGGEKKTLRIAAEYADYTNFSGMPEE
                     FSAKSEILRAHCADVGREFEDIVRSANFNVVIGRDDAEVQERLAWIRDHYTSAGLPED
                     VVESTVKSFADGPLVGTPEQIIEALVDLRSRGMTYAITYFVEQAYDLSGVELFEQAVI
                     PAFMEREQHGGMWHFLHGR"
     gene            19901..21073
                     /locus_tag="PL014_00595"
     CDS             19901..21073
                     /locus_tag="PL014_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOT55616.1"
                     /translation="MSRTICVVGTSYVGLSIAVLLAEHHDVVAFDIDESRLEQIRAGR
                     SPIADPDIEERLARSDLRLTVTNDKHEAYSRAEYVVVATPTNYDERTNYFDTSSVEQV
                     VSDVVTINPDATAVIKSTIPVGFTAGLRRRLGTDNIIFSPEFLREGRALHDNLHPSRI
                     VVGDQSPRGERFAQLLAEAALDDDVPVLLTDSTEAEAVKLFANTYLAMRVAFFNELDT
                     YAATHGLDTRQIIEGVGLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYADVPQTLIS
                     AIVTANTTRKDYVAADIVARAPRVVGIHRLIMKAGSDNFRSSSVQGVMKRIKAKGIEV
                     IVYEPELSDDRFYGSEVVRDLDELKSRSDVIVANRLVDELRDVADKVYTRDLFGND"
     gene            21092..21544
                     /locus_tag="PL014_00600"
     CDS             21092..21544
                     /locus_tag="PL014_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOT55617.1"
                     /translation="MGLKHQNDPDLVRGLLMDPGTWVIVGLSANRDRVAYGISQWLHV
                     ELGKSIIPVHPKAETVHGARGYASIADIPDQDVKVIDCFVSSEHVGAVVDEAIAHKDR
                     LRIDAVWMQVGVVDAAAAERARAAGLDVVMDTCPRHEWPKVRSEGMQR"
     gene            complement(21552..22109)
                     /gene="purE"
                     /locus_tag="PL014_00605"
     CDS             complement(21552..22109)
                     /gene="purE"
                     /locus_tag="PL014_00605"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT55618.1"
                     /translation="MSRQEQGAEVTGQVPVVGVVMGSDSDWPVMQAAAAALDEFGIAC
                     EVDVVSAHRMPQEMVGYGASAQERGLRVIIAGAGGAAHLPGMLASVTSLPVIGVPVPL
                     KYLDGMDSLLSIVQMPAGVPVATVSVGGARNAGLLAARILGAGEGPEAARIRDALTTF
                     RGALRDEAHAKGAALRERRADGIRD"
     gene            complement(22106..23287)
                     /locus_tag="PL014_00610"
     CDS             complement(22106..23287)
                     /locus_tag="PL014_00610"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOT55619.1"
                     /translation="MTTPRRAPGGFPVVGIIGGGQLARMCAGPAAELGITLSVFAEAE
                     DASAALVVPSSPVGDHTVAEAVHAFAQHCDVVTFDHEHVPAHVLDLLVAEGVELHPRP
                     AALRFAQDKLAMRERLTELGIPCPRWTRADDVAEVTAFGDAVGWPVVAKTPRGGYDGK
                     GVRLAASADDLADWLERAATDGTGLLLEERVPFVRELAVLVARSPSGQAAAWPVVETV
                     QTDGICTEVLAPAPGLDADLAADAVHAALRLAGELDVTGVLAVEMFEVVDAGGPAFRV
                     NELAMRPHNSGHWSMDGAVTGQFEQHLRAVLDLPLGSPRPHERWTVMANVLGGDYPDL
                     YPTYRHVMARDPEAKVHMYGKGVRPGRKIGHVNISGDDLADLRERAAHAADYIQGVVT
                     E"
     gene            23327..23803
                     /locus_tag="PL014_00615"
     CDS             23327..23803
                     /locus_tag="PL014_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT55620.1"
                     /translation="MSHPPSTPTRGPARLMAWARGAMDVIYREMIKFGVIGALAFVID
                     LGLANLLWHTVLEDRVTTAKIISGLVATLFSWVGNRQWTFRHRRSRPAHHEVALFFGV
                     NIVALGIATVTLAFSHYGLGFTSRLADNVATIVGIGLGTLFRFWTYRRFVFASEKI"
     gene            complement(23805..24836)
                     /locus_tag="PL014_00620"
     CDS             complement(23805..24836)
                     /locus_tag="PL014_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT55621.1"
                     /translation="MRRLLVWTAVQAVAISGVIITVGFSIFILWRDDPSESIISAGQQ
                     TAREASSPLLSLWAHLVALLVATALALAVAAWVADRRALRVSHFLRTLGERAERLAPG
                     DPRPVPLNSGIEEVDRLDAALARGAQQGAKRLASERDFAADASHQLRTPLTALLMRLE
                     EIASTDDLTVVGEEATIAIGQVERLSRVVDDLMSRTRSGVETNPAVSLDSVLASLQRE
                     WQPAFAAARRSIHVSGERGLRVRATPVALAQILTTLLENSLAHGAGTVEVAARRSGPS
                     VVIEVSDTGAGVAPTLAPHIFERAVSSSGSGLGLSLARDLAEAAGGRLELARAVPPLF
                     ALFLSVSED"
     gene            complement(24843..25514)
                     /locus_tag="PL014_00625"
     CDS             complement(24843..25514)
                     /locus_tag="PL014_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT55622.1"
                     /translation="MTKVLLAEDDPAISEPLARALRREGYEVDVVTDGESALAQALLA
                     PDLVVLDLGLPKMDGLEVCRRLRAQGGTVPVLVLTARADEVDTVVGLDAGADDYVTKP
                     FRLAELLARTRALLRRGVVEPAADSLVRIDPEGRRVFLRGQEVPFTGKEFELLKLLVA
                     NEGKVVSRERIMREVWDSAWYTSTKTLDMHVSVLRKKLGDDASQPSYITTIRGVGFRF
                     DAPQE"
     gene            complement(25511..26617)
                     /locus_tag="PL014_00630"
     CDS             complement(25511..26617)
                     /locus_tag="PL014_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT55623.1"
                     /translation="MTTEEVSGARDHPLVEELPGQIERRLLGRPASMGRREVSEGAGV
                     EPVVARRFWHAMGFQNVDDEDAMFTEADLEALKRVARLIGDGEVSEELALGMTRAFAR
                     TSDRLAVWQTQLVAESLTSPFSEELEGKDSRSVPEPRIAADAAELLANICDDIEPLLV
                     YVWRRHLTNAIARMIADADPAVHSDPSAPIRVVGFADLVSFTSFVRRMSERQLARLVQ
                     RFELLASDVITEHGGRVIKTVGDEVLFVHTDAAAASAIALDLVDAMAEDELLPPVRVG
                     LAHGRVVSRLGDVFGLTVNKASRITAVTPSGHVFVDEDMAKVLRSVSGFSATERRRRM
                     LRGIGVVTLHELQRAPGGRGPALREIDARLPSKP"
     gene            complement(26628..27395)
                     /locus_tag="PL014_00635"
     CDS             complement(26628..27395)
                     /locus_tag="PL014_00635"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT55624.1"
                     /translation="MSQNPDQRPLSERSVTPGAPWPPIEVFDRLGSTNDEVRSAPSPW
                     RVVVAEEQDAGRGRLGRTWTTTPGTSLAVSVFVPPPASGPSWVPLLAGLAVRRAVAEV
                     AGLETALKWPNDVLVPSDGDRKLAGLLCEWMADGVVVGLGINVDTAREDLPLDTATSL
                     RAAGAPGVDRAALLSAFLTHLAALLRDDTGPGGSAQAAYVAACSTVGRVVEVHLPGGA
                     VVHGIGTGVDAAGRLTVRDESGTQSVSAGDVVHVRSR"
     gene            27419..29047
                     /locus_tag="PL014_00640"
     CDS             27419..29047
                     /locus_tag="PL014_00640"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT55625.1"
                     /translation="MPSSDTKAIGGTDVPKDIDTTTTAGKLADLKRRVAEVAGAGSER
                     AVEKQHARGKKTARERLELLLDEGSFVEMDKYARHRSVAFGQDRNRPYGDGVVTGYGT
                     VDGRTVAVFAQDFTVFGGSLGEVFGEKITKVMDFAMKVGCPVVGLNDSGGARIQEGVV
                     SLGLYGEIFRRNVHASGVIPQISLIMGPCAGGAVYSPAVTDFTVMVDQTSHMFITGPD
                     VIKTVTGEDVGFEELGGARTHNTTSGVAHYMGADEDDAIEYVKALLSYLPSNNMEQAP
                     VFGEENDLEVTDDDRFLDTIIPDSANVPYDMHEVITRVLDDGEFLEVQPLFAPNLVVG
                     FGRVEGHSVGIVANNPMQFAGTLDINASEKAARFVRTCDAFNVPILTFVDVPGFLPGT
                     DQEWDGIIRRGAKLIYAYAEATVPLVTVITRKAYGGAYDVMGSKHLGADINLAWPTAQ
                     IAVMGAQGAVNILYRKELAAAAERGESVEEARATFIKQYEDALANPYIAAERGYIDTV
                     ITPSNTRMNVTKALRALRTKRASLPPKKHGNIPL"
     gene            29112..29357
                     /locus_tag="PL014_00645"
     CDS             29112..29357
                     /locus_tag="PL014_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HOT55626.1"
                     /translation="MGPGPMTASQVPDAVTPVIVRGAATPEEVAALVAVLSAVGGSSD
                     GSSAADAASSVSAWATPAATMRRPVGYGPGAWQNSLR"
     gene            29554..30486
                     /locus_tag="PL014_00650"
     CDS             29554..30486
                     /locus_tag="PL014_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55627.1"
                     /translation="MPDTSPPLPGPFLRAEAKNLGVSDRRLRSPDFRTVFTGVVVDAR
                     IPDTVVVRSRAAMLVCPEGGVLSHWTAARLWGGVVPDNEWIHVAFMRDVRFRVSGAKP
                     HRFRHRLDIRFRHGLPVTSPAQTFCHLARHLGLVDLVALGDRLVKKGRCTPDDLIAYA
                     QNWPGQCRVEALSAAKLVRKGVDSVPESALRLLMVLAGLREPEVNIRLRDVEGNVRFR
                     IELGYEDALLAIEYDGRWHEEPAQKAHDDERRSGLRERDGWTFVIVTADDLYGLPEQL
                     LERIRSAMESLGIPVPAVLSDGWRRHFRVDTAAA"
     gene            complement(30590..31261)
                     /locus_tag="PL014_00655"
     CDS             complement(30590..31261)
                     /locus_tag="PL014_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOT55628.1"
                     /translation="MKPVVTSLNIGGARSGIRPRGGDTAIDKRPVDTIEVRDPGPKRG
                     GLGSGVLGDDVVSRKHHGGSQQAVYAVAREELDWWGAELGRDLRDGMFGENLTTLGLD
                     VDAAVVGQRWTVGSAVLEVTGPRIPCATFAAWMGERGWVRRFTERGRTGAYLAVVEPG
                     VLSVGDTIEVGQPPEHGITVPEVFGAFMGDDDLAARVVAAQVLAPADSLDLERSLARR
                     RQIDQ"
     gene            31413..33101
                     /locus_tag="PL014_00660"
     CDS             31413..33101
                     /locus_tag="PL014_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HOT55629.1"
                     /translation="MTEPTAEPRTTTAVDAVADAYFDAAIVASPIEATYLGVPGRDTE
                     LDDLSPDGFAHHAHLARRTLAALDGVEPADAVDRVTVAAMRERLGLVIETHDAGYDAM
                     ALNVIASPLQACRDVFDLMPTGTQEHWATIAARLSAVPQAMEQYTQTLLDSADRGLVT
                     PRRQVEACIVQCEELTAPDGFFATFAQGASADGAPLADSVRADLDRGARAAAQAYEQI
                     GTALRERLLGLAPTSDAAGRERYGLASRSFLGATVDLEETYAWGQEELARITDDMART
                     ADRILPGASVREAIAHLESDPRYQLHGTDALRDWMQERADEVIAAMADTHFDIPEPVR
                     TIECLIAPTQTGGIYYTGPSDDFSRPGRMWWSVPKGVTEFGTWRELTTVYHEGVPGHH
                     LQVGQTVYRRELLNKWRRMMCWTSGHGEGWALYAERLMAELGFMDDPGNYLGLLDGQS
                     LRAARVVLDIGVHCGFEAPAEVGGGSWTYDKAWTFLRAHSNEGEEMLRFELNRYLGWP
                     GQAPSYKVGERLWLQLRDEAQQRAGDAFSLKDFHRRALDVGSVGLDVLRDAVLD"
     gene            33101..33733
                     /locus_tag="PL014_00665"
     CDS             33101..33733
                     /locus_tag="PL014_00665"
                     /inference="COORDINATES: protein motif:HMM:NF037150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT55630.1"
                     /translation="MGSEPAGDGRGAVARGRRPAGSGTREAILAAAARAFAEQGYPRT
                     TLRGIAKDAGVDTRLVSHYFGSKQELFVSVVELPFDPDVVIPALLGPGRTGVGFRLAG
                     FAIGIMDTPEARQTMTGLLRAAASEEAAAVLVRDLLMERLLGPLAHHLGGDDPTLRAS
                     LAASQVAGMAFTRHVVGLPRLATASRDELVGALGPVLEHYLVGDLSPPPA"
     gene            33751..35646
                     /locus_tag="PL014_00670"
     CDS             33751..35646
                     /locus_tag="PL014_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT55631.1"
                     /translation="MELASAIVAIVLVVIVVSRLAAPVGVPTPIALLLAGAVLSFVPA
                     VPQLTLSSDLVLYGLLPPLLYAAALSTSLLDIRANRASILGLSVGLVLFTALGVAVVA
                     WLLLPIPFAVAIALGAIVAPPDAVAATSVARSIGLPRRVTTILEGESLLNDATALVTL
                     RTALAAAGLAAHGAATHGEVPEVTVPSVATDLLIASVGGVAIGFAAFLAIGALRRQLH
                     EVPADTALSFVAPYIAYVPAELVGASGVLSVVTAGLLLAHKAPILQSAPSRLAERINW
                     SSVTFVLENAVFLLIGLQIASLLEAVGEGDGSLGRTLLVGGAVLVTCLLLRPLWIVPF
                     SLIGARRAPDRSDGIREGLVGSWAGMRGVVTLAAALTLPEETPLRAELIVIALVVTVG
                     TLLLQGLTLPSLARALDVRGPDPREDALVEATVLGATTGAGLRLIEADPAADPTAVAA
                     IRDQASARVNRSWERLGTLGPGDDETPSEAHARLRTKMIREERAELMRIRGEGHVDHA
                     VLSAVLGQLDAEETALVWSATRSGAVRASPLRPPDRIAGACEHLAAAEVPKAPTTAEG
                     CPRCLADGVRWVQLRACATCGEVGCCDSTPGRHAEAHFRETGHPVMRSIEPGEAWRWC
                     YVDEIVG"
     gene            complement(35622..36674)
                     /locus_tag="PL014_00675"
     CDS             complement(35622..36674)
                     /locus_tag="PL014_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOT55632.1"
                     /translation="MSDLSTSYLGLALRNPLVASASPLSEGVDRVRALADSGVGAVVM
                     YSLFEEEVHREQLRDLMVSEAHEDAFGEALSYFPNVPSAGTGGATRYLRLLEQSAAAV
                     EVPVVASLNGSTAGGWVGFARSMQDAGAAAIELNVYFVPGDPHTRARDVEDRHVEILQ
                     QVREAVSVPVAVKLSPHFSSTGEMAMRLDRAGANGLVLFNRFLQPDVDPEALAVTTGV
                     TLSTPAEARVARTWIAILRGHVTASLAATTGVDGAADVVSYLLAGADVVMTASALLRH
                     GVGHAGVLLDGLEDWMRRKGFATPEEFRGLLAVPVGVDQSAYERAGYVEALEKARRTY
                     GDLTGASRGQPTISST"
     gene            complement(36675..40259)
                     /gene="nifJ"
                     /locus_tag="PL014_00680"
     CDS             complement(36675..40259)
                     /gene="nifJ"
                     /locus_tag="PL014_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOT55633.1"
                     /translation="MRVTIDGNEAAVDVAYRLNELCAIYPITPSSTMAELADEWASHG
                     RANVWGTVPTVMEMQSEGGAAGAMHGALQGGALSTTFTASQGLLLMIPNMYKIAGELT
                     STVFHVAARSLAAQGLSIFGDHSDVMAVRQTGFALLASASVQEAHDLALVAQAATLRT
                     RVPFVHFFDGFRTSHELNTVELIDDEVLRSVVPEALVREHRARALTPEHPFVRGTAQN
                     PDVYFQARETVNPFYARTAAVVQDVMDEVAGHTGRAYRVVEYHGHPEAERVVVVMGSA
                     AETVRETVAHLVAGGDRVGVLQVRLYRPFPSAELVEALPDSVRAVAVLDRTKEPGSNG
                     EPLYLDVLATLAEAGSRGERDGIPRVIGGRYGLSSKELTPGMVAGVFAELALEAPRRR
                     FTLGITDDVGGTSLPYDATLDIEDPQTLRAVFYGLGSDGTVGANKNTIKILGADPEVH
                     AQAYFVYDSKKSGGLTVSHLRFGPHPIHAPYLVSRAGFVGCHHLGLLDTVDVLACAQE
                     GATLLLNAPQPPDEVWDAIPGPVQQGIIDKRLRLYAVDATSVARGAGLPGRTNTVLQT
                     CFFAISGVLPADEAVARVKAAIRKTYGRRGAEVVRRNEAAVDATLAALHEVPVPAART
                     SLRDLPDVVPRDAPPFVRTVTAEMMAGRGDALPVSALPVDGTYPSGTSAYEKRRISDL
                     VADWDPDTCIQCGTCSFVCPHSVIRSVFYDGDALDGAPEGFRSAPLNAAGLPGSRYTL
                     QVYVEDCTGCGLCVESCPVSPLVVPGMPGAPERKAINLAPLEPVLEAERENIAFFETL
                     PVNDRSRVDFGTVRGTQFLQPLFEFSGACTGCGETPYLKLLTQLFGERLTVANATGCS
                     SIYGGNLPTTPWTKNAAGRGPAWSNSLFEDNAEFGLGLRLAADLHTELARERLGQLRD
                     QVGADLADGILEAGQVREFELTAQRERVAELLRRLEGMTGPVVEDLRSVADHLLRRTV
                     WIVGGDGWAYDIGSGGLDHVLASGRDVNVLVLDTEVYSNTGGQSSKATPIGAVAKFAA
                     AGKSVAKKDLALQAIAYGTVYVARVAMGADPQQTLTALRDAEAYDGPSLVIAYSHCIA
                     HGIEMRQGLDQQYRAVASGHWPLVRYDPAVRASGGNPFQLDSPRPRLPLEDYVYHELR
                     YRMLRNSDPAEAERLLRLKQEAVDQRWQTYEEMATRGADRFAADTRRT"
     gene            complement(40264..41895)
                     /locus_tag="PL014_00685"
     CDS             complement(40264..41895)
                     /locus_tag="PL014_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOT55634.1"
                     /translation="MAGEDRDLTPLASVGQGRPRSGPVRSQRPVYVDLLPPCNAGCPA
                     GENIQAWLALVKGGDPEAAWRRLTLDNPFPAIHGRVCYHPCEAACNRADLDGAVSIHG
                     VERFLGDQAIEHGWAFDPPPPATGHRVLVIGSGPSGLSAAYHLVRLGHAVTVRDVGGE
                     PGGMMRYGIPTYRLPRAVLDAEIDRLRDLGVVIEQGHRVEDLEAEMRDGSFDAVFVAV
                     GAHLSTRVDIPNQDASRVMDAVEFLRGAASGTQPPLAGRVAVYGGGNTAMDAARVARR
                     LGADDTVIVYRRTRDQMPAHESEAQDAEEEGIRINWLRTISSMGDTDLTVEVQELDEH
                     GRPHGTGRFETLEADTVILALGQASDTGFLRGIPGLEFQGDTVQVAKDTLMTGAPGIF
                     AGGDAVPSQRTVTVGVGHGKRAARAIDAWLRGDAVHRAPKHPLASLDQLNLWYFGVHD
                     RREEAVLDPATRALTFDEVLTGLSPTEADFEAGRCLSCGNCFECDGCLGACPEGAVIK
                     LGVGLRYEFDDARCTGCGACYRQCPVHAIDMIPEG"
     gene            42017..42721
                     /locus_tag="PL014_00690"
     CDS             42017..42721
                     /locus_tag="PL014_00690"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOT55635.1"
                     /translation="MPSARLTLLLASASPARRTTLIAAGVTPHVRVSSVDEDAAVAAA
                     EAMSGPLEAPDVALLLARAKAEDVVSTIEADETGDTDGADETDDLPDDLLVLGCDSVL
                     ELDGEVHGKPADAAEAVERWRRMRGRTGTLHTGHWLVDARHADDGGTGGMVGGTASTV
                     VHFADLDDDEVEAYIATGEPLAVAGAFTIDGLGGPYVTAIEGDPSTVVGVSLPLLREL
                     VRELGLAWHDLRTPTP"
     gene            complement(42740..>43779)
                     /locus_tag="PL014_00695"
     CDS             complement(42740..>43779)
                     /locus_tag="PL014_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT55636.1"
                     /translation="AYIPSFCDLIIMVEGNASMYLGSPRMAEMVVGEKVSLEEMGGAR
                     MHCTVSGVGDLLAHDDAEAIELARLYFSYLPGNWRGPLPTYHAEEPSAPLTRASVPER
                     ESQPFDIHEVIDGLVDEHSFFEIKPLFASELVVGFGRIDGESVGIVANNSAVKGGVLF
                     SDSADKATRFIWLCDAFSVPLIYLADVPGFMIGSEVERGGIIRHGAKMVSAVASATVP
                     QFCVVVRKAYGAGLYAMGGPGFGPDATIALPTARIAVMGPEAAVNAVYANKIAEVEAA
                     EGVQARDAFVAARRAEYEADVDLERLAADLVLDQVIEADALRDELRRRLNYAARRDRH
                     FSDKRRDVPPV"
BASE COUNT         6354 a        15827 c        15270 g         6328 t
ORIGIN      
        1 tgatccccgg gaagatggtc aagggcatgg gcggcgcgat ggacctcgtc cacggtgcca
       61 agaaggtcat cgtcctcatg gagcacgttg ccaaggacgg gtcgcacaag atcgtcagcg
      121 agtgctcgct gcccctcacc ggcaaggccg tcgtgcagcg gatcatcacc gacctcgccg
      181 tgctcgacgt cacgcaggac ggtctggtcc tgcgtgaact ggcgcccgac gtcaccgagg
      241 agcaggttcg cgaggcgacc ggagccccgt tcagcgtcgc cctggactga cgacgcgagg
      301 ccctccggcc ccggggagac cctctaggag accgtccacc acgtgctgcg caggcccagc
      361 gcggcgctga aggccgttcc cttcacggtc ttcgagcccg ccgagccgat gaccgtcatg
      421 gtgatgatgc gcccgcccca ggcatcgatc ccgtcacgct tgtcgatgcg cagcgccttg
      481 agcttcccga tcgtggggta cgccttctcg atcgcgctga tcggcaacgt cttggtccac
      541 cggctcgtgc tgcccgacgg gaccgcgtcg taggggtccg ccaacgacac ctggtacggc
      601 agcgcggtct tcacggtacg gccgccgttc gaggacccga actcggtcag agcgggagac
      661 ccgttggcga agagcccgag gccggccgtc gaggcgaccg ctgccgtcga cctggcgttc
      721 tcgtggggga cctgcgtgcc ggctgtcgtg tagcccgcca tgcctcggta gacctggcag
      781 ctcgtcgagt cacaggtgtc gtacggggtc ccgggcgcct ggttggcacg ctggtgggca
      841 gcataggttc gtgcggcgac cacctgcgcc tcgagcgccg ccgtcggcca ggacgccggc
      901 atctcggacg gcaccacgct ctggaggtag tagtccatcg acaccacgct cagcgtcatc
      961 atccccgccc cccagcggat gccgcgcacc gtacggcggt agtcgcgctg ggtgccgtcg
     1021 ggcaggacga cgcgcaccgc gccgagcgat gcgtccgtga acgacaacgg caccgttcga
     1081 gcccgccagc tgctcgtgct gcgccacgag cccttctcga gccactgaag ggtcaggccg
     1141 gtgccctcgg gcaccagacg ccaccgctcg cgcggggtcc cgtcggagtt gcgcacggcg
     1201 agggcgccgg tcttggtgcc gtccgagacg accaggcccg gtgtggtcac gagctgggtg
     1261 gtggtggatc cgagggcggt cagacgcacc cgcatcgtcg ggttgccgat gctcgtggat
     1321 cgggtcgtgc ccgggtagta gaaggacaac atctcgttgc ggctcaggcc cttcagggcc
     1381 gctccgtagg cgccgtactg gctcatcccc cgtccgtgcc cgtagccacg gccggacaag
     1441 gtgaacgacg tgccagcagg ccgggggtag gtggtggagg ccgaggccgt ggcggcggag
     1501 ggcgcggacg caccgacgcc tgccgcgctg ctcgcggagc cggtgaagac ggcgcccgcg
     1561 gccacggcga acgcgacagt gacccgaagt gctctcatgg ctcctcgccc ggaaatctcg
     1621 gtacggcccg gcacggaccg gctcaccccc aagagggcgt gggttcggcc acgttggccg
     1681 accggtgacg ccgcagtctg ccacagcagc cacttcatcc tccatggcca cagccgatcg
     1741 gatctgctgc cctcgctatg ctgcccgcat ggcatccacc ggggacgagc cacccggcgt
     1801 cggtggcagc ctgcgctcgc gcgccgcgct gagtaccatt gccaccgcgg tgcaggggct
     1861 ggggcgcctg ctctacagcg tgctcatcgg ccacctcggt tcgcgcgaac ttctcggcca
     1921 gacgaacacc tcgttgtcgc tctcggtgct caccagccag ctgtgggcag cccccgcggc
     1981 gacggccggc acccgcttcg tggccatgaa ggcgaccctg gatgaccccg agggtgccgc
     2041 gacggtggcc cgccacatcg ccacccgcac cgccctgatc agcatggtgc tgccgaccgc
     2101 ggtcgccctc gcggggtcgg cgtggctggg gttcggtgcc gcgcacacga tcggcacggt
     2161 ggtcctggcc ttcacgtatt ccatgtacgt cacgctgcgc ggggtgcagt acggagcgct
     2221 gcgctttcgc cacgtcgccg tgtgggacgc catcgccgcc gccacggcac tgctgaccgt
     2281 caccgtcgtc ctcgccctcg acctcaccgt cgtggccctg cttccgctga ccctgggcta
     2341 cgccctgttc gcggtcgcgt cgtggccggc ccgggtcgct gctcgggtcg aggccccact
     2401 gcgccgcgag atcgaccgtt tcgtcttctt cggcgcggtg agcgggctcg ccagtggtgg
     2461 cctgctgcag ctctcccagc tcgtcgcgca ctccgtcgcc gggccggccg aggccggtga
     2521 cttcgcggcc gccctgaccc tggccacccc ggcgtcgatg ctctcgatcg ccctgagcac
     2581 ggttctcgtc cccccactcg tggccgccgc cgggcgtggt gaccgcgccg ccgtgcacgc
     2641 gcagagcgac gcgatcgcgc gccggctcac cgcgatcttc gtgggcctgt tcggggtgct
     2701 cgtgatcgtc agcccgctgg gcatcgcgct cgtctggggg gaggagtacc gcgccgcagc
     2761 aggcatcctg ccggtgctgc tcctcgccgt catgctcact tcgatcgccc tcggcgccgc
     2821 caccaccttg cagtcgaccc gggcccgtgg accgcgggcc gtcgcatacc tcaacctcgg
     2881 tggcctggtc gtcagtctcg ccgtgtggcc cgtgctcgca ccgcgtctgg gcacgaccgg
     2941 ggtcgcgctg ggctacctcg tcggggcagg cgccgcctcc ctgggcgccc ttgcgaccgt
     3001 gtggtgggtc gagcggcacc ggtggctgga cctggccgcg cgactcggtg ggggcatcgc
     3061 cctcgtcgtc gtcctcgcgg ctctcgcccg ctcggtcgac ggggtgggtg gtgccgtggc
     3121 ccaggtgggg gccgcgctgc tcttcggtgc cgtctggctc gccctcaacc gtcgtgaggt
     3181 cgccgcgctg tgggccacgg tccgggggtc gggcggaacc ccgaccgcct aggatcgacg
     3241 cgtgccgccg accctcaccg tgctcatgac gacctaccac gggacctcgg tcgaggagct
     3301 cgagaggtcc atcgcctcga tcaccggcca gacccggact cccgacgagt tcctcgtcgt
     3361 ggtcgacggc ccggtgccac agccgctcga cgacgcgctc gcggcggccg ccgcggtgca
     3421 ccccggcatc cgggtggaac ggctcccgac caacgtcggc agcggtctcg cgtcggcgcg
     3481 ggggctggaa ctggcgtccg gggagttcgt cgcccgccac gactccgacg acgtctcgct
     3541 cccccaccgc ctcgagcgcc agatggatgc cgtgcgcgat caccacctcg acgtcgtcgg
     3601 gtcctcgatg ctcgagttcg agggctcccc cgaccacgtc gtcggcctgc ggcgcaaccc
     3661 gcgcacgcac gagcagatcg ccgcccgcat gcgactgaac aacccgatca acaaccccac
     3721 ggccgtcttc cgcagggagc tggccctcga ggtcggcggg tatgccgacc tgcgctacat
     3781 gcaggactac gacctgttcg cccggatgct cgcggccggc gcccgggccg agaacctcga
     3841 tgagcccctc gtgctcttca acgccggtga cggcatgatc tcgcgccgcg gcgggtggcg
     3901 gatgctgccc tacgagtggc aggtccagcg gcgcctgcgc gacaccggca cgatcggcaa
     3961 cgtcctgctg gcgcgcaaca tggtcgtccg cgggacgttt cgggtgatcc ccccgaccct
     4021 gctcaagcgg gtctacgccg tgctcttccg ccgggggtcc ggcactcccc cgagggacac
     4081 cggcgcctcg ggcgcgacgg gcacgccatg agcggcgggg tcgggcgcgg gcccgtgctc
     4141 acctgggtca tcgtctccta cgggggcgtc gacgacgccg tgcgcctcgt cgactccctg
     4201 ggcgccggct cacccgtcga catcgtgctg tgcgcgaacc ggccgggtga caccgacgag
     4261 gccaccgcag ccttcgccgg cgaccgccgg gtccgggtgc tctccttcga ggacaacccc
     4321 gggtacctgc cggcgctgca acgggccctg ccgcacgtcg acacgacccg ccccgtcgtg
     4381 ctctccaact gcgacctgct cgccgacccg ggcgtcgtcg acgacctgat cgaacaggtc
     4441 gaggcgttcc cggatgccgg gtggctcgct cccagcatca tcggcagcct cggtcaggac
     4501 cagaacccca acctcgtgca ccctccgtcg gcgcgctggc tgcgagccct ggcagcgatc
     4561 caccgcttcc caccgctcac cgacctgctc ctgctgcgca aggaaggtca cagctcgcgc
     4621 accgtggccg gggtcgacgc cggcagcacc gtcttcgcgg gccacgggtc gtgcatcgtg
     4681 ctgaccccgg cgttcttcgc ggccggcggc tcgtgcgact acccgttcgc cctgttcggg
     4741 gaggaactgt ggttcggccg ggagtgtgcc cgcctgggcc tgaccgtgcg ctacgtcccc
     4801 agcgcggtgc tgcgccacgc ggagcacgca gccaccggtc gacgtcggcg cgggcacgtg
     4861 gctcgggtga agtacgaggg cctgcggtgg tgggctcgcg aagcccgggc acagcggtgg
     4921 tgaccgccca ccgtgcgtcg ccgggagcac gcacccggat caccgtaggc tgtccacgat
     4981 gagcagcgcg caccgcacca acgacgacgt ctggctcgtg gtccccctct tcaacgagga
     5041 gaccgtgatc gccgacgtcg tccgtgacgc ccgcaagacc ttcccgaaca tcgtcgtggt
     5101 cgacgacggg tcatcggacc acggggcgga tgccgcggca tccgccgggg cgatggtcgt
     5161 gcggcatccc gtgaacctcg gccagggggc cgccctgcag accggattcg agtatgcgct
     5221 cggagtgccg acgatgcgct gggtggtgac cttcgactcc gacggccagc accaggtctc
     5281 ggacgtcctc gccatgctcg acaaggcccg caccgaggac ctgcgggtcg tcttcggctc
     5341 gaggttcctc gactcccgca cgaccccgag cttcatgaag aagatcgtcc tgcgcctcgc
     5401 ggtgggttac accaacgtca cgaccggcac caggctcacg gacgcccaca acggcctgcg
     5461 actgctgcac cgcgaggtcg tcgagcggct cgacatcacc cagaaccgga tggcgcacgc
     5521 ttccgagatc gttgcccaga tcggcgacat gaaggtgccc tacggcgaga gcccggtaca
     5581 catcctctac accgactact cgaagtccaa gggccagtcc ctgtggaact cggtcaacat
     5641 cctcgtggag ttgatgttca ggtgagcacc gtcgtcatca tccaggtcgt cctcatcacc
     5701 ctcgtggtgc tcgtggtggc acggctcttc cggtcacgcg gtgccagggc tcaggcgatc
     5761 cggcgcatcg gactggtcgt cttcgcggcc ttcgccgcgg tctcgatcct cttccccgac
     5821 atgtggacca agatcgccca gaaggtcggg gtcggtcggg gtgccgacct cgtgctctac
     5881 gcgctcgtcg tggcgttcct cagcttcacg gtgacgacct acctgcgttt tcgtgacctc
     5941 gagacgaact acacgcggct cgcccgcagg atcgcgctgt cagatgcacc ggctccaccg
     6001 aaccaccccg acgaccctcg cgcatgaccc tgggacccct gctcgagcgg gccggccgtt
     6061 cgctggtcac cgcggccctg gtcctcttcg ccgtctggac cgtggtctac caggtcgccc
     6121 tggccgtcgg cctgccctcc acgcccacgc tcgtcgtcgc cctgctgatc ggggccggca
     6181 ccctggtgct cctgtcccac acgacgacct cgaacgactc cgtcgtggcc cccgtaccag
     6241 gcgccgcagt gtccctggcg gtcctcggcg tgaccgtcgt cgccacggcg ctcggcgcct
     6301 acggcatgag gggcccggcg ctcgcgctcg ccgtcctcac cgccgtgacg gcgctcgccg
     6361 tgacggcccg tcgcggcgcc ccaccggcgg gacaggcggc ggcgaccggc ggcccggcat
     6421 ccgacgacgc ctcagccgcc gacccacgga cgtggacggt cggctgggtc ggcgccctcg
     6481 tgagtgccgc cctggccagc atcatcgccc gaccagacgg cgacgacgcc tacttcgtca
     6541 atctctcggc atgggtcgcc gaacagggtc gcttcccgct tcgggacacg atgatcagcc
     6601 cggacgtcta cccggcgctc ggagcccact cgcctccggt ccactcgatc gagggcctca
     6661 tcggtgccct ggcccgcgtg ctgcacatcg aggccggcac cgcgacgtac gtcctcgtgc
     6721 cacccttggc cacggctctg gcagtgctcg tcctgacccg catcgtgacg gccgccagga
     6781 tccccgccgg tccggtggcg ctgctggccg ccctgggctt cctgtggacc acgggaggca
     6841 gcggctacag cttcggcaac ttcttcgccg tccgcatgtg gcagggcaag tccatgctcg
     6901 tgtcgatcgt catcccgctg gtgatcctgt tcggtgtcga gctgatccgt cgcgggagtg
     6961 cccgagcgca cctgctcttc ggcgcctccc tcatcgctgc ggtggggatg tccaacaccg
     7021 cggtcttcct cgtcccggtt ctcgtcgggg gactcgtgct cgccgcgctc gccctgcgcg
     7081 agatccgcgg tgcggtgcgg ctgagcctgt gggtggtgta cccgatcatc gccgggctgg
     7141 ccaccctggt gttcgcgacc ccttcaccga cgaaggccca gctcgacgtc gagggattcg
     7201 tcctggcggc cagccgagcc ggggacccgt tgatgacggt gccggggcgc cacggcatct
     7261 acgtcgtctc cgccctggcc ttgggcctcg gcatcctcgg ccttcgcgag gtggggctgc
     7321 gcacggctgc catcggatcg ctcgccgccg ccggggtgat gctgcttcct ccggtgcgcg
     7381 ggatcctcga ggggatcggc ctcacctccg tggtgtggcg gatgtggtgg gtcgtgccca
     7441 tccccctcct cgtggcgggt ctcgtgggcg ccgccgcgct cttcctgccg cgcggatggc
     7501 cacgcgccgt cggaggcctg ctggccatgc tcctcgtcgt gctcgttccg ctcgtgggcg
     7561 gtcgctgggt cggctccgag ggcaacaacg cccgcttcgt ctccccgatg gcctggaagg
     7621 tgccgcgggg agcgctcagc gaggcccgtt tcatcgagca ggtcagccaa cccggggaca
     7681 tcgccctggc tccctgggac acctcacgcg tgctcgcagc cctcacggtc gacgtgcaac
     7741 ccgtctcggc ccggcgcgcc tacctgccca cgtatgccgg cacccccgag gcgcaggccg
     7801 gcagccgcga ggagctgcag gagttcgccg acgagcgcac ccccggcacg gacaccatcg
     7861 gcgacgacct cgacgccgtg ggcgtcgaca ccgcctgcgt gggcacctcc cgcggacgcg
     7921 ccatcgacct gctcgaggcc aacggcttcg aggtggtcgg cgtcatcaac aacctgacct
     7981 gcctgcgccg ctgagccgcg ctcggccccg ctggaaccct cagcgctgct gggtcccctg
     8041 gtcctcgttg tagcggtcga gcacctcgtc catgggcccg tccaggacca gctgcccctg
     8101 tcgcaggtac aggcccctgg tcccgaaccg cttgaggtcc cgctccgagt gcgagacgag
     8161 gaagagggtc cgcccctccg acagcaggct ctccatccgg gtgtagcact tctcccggaa
     8221 ggccttgtca ccgaccgcga gcacctcgtc gacgaggaag atcggttcat cgagcgagga
     8281 gatgaccgcg aaaccgagcc gcacctgcat cccggaggac aggtggcggt acggccggtc
     8341 gagcgccgcg cgaacctgcg ggccggcgaa gtcgacgatg tcctcgaagc gctcctcgat
     8401 ctgggccttg gacagcccgt gcaggccggc ctgcacccag aggttgtcgc ggcaggtcag
     8461 ctcgccgcgg aaacctcccg tgagctcgat gaggggggcg acccccccat tgacctcgac
     8521 ggtgccgcgg tcgggcagga gcaccccggc gatcgtcttg agcagggtgc tcttgccgct
     8581 gccgttaccc ccgaccaggc ccaccgcctc gcccctggtg atctcgaagg agatgtcctt
     8641 cagcgcccag aaggtgtccg tggacgtcgt cgcacgaccg tggaacagca ggtcgcgcac
     8701 ctgcatcttg cggcggcgcg aggtgtagaa ctcgaccccg acgtccttcg ccgtgatgac
     8761 cacgctcatt cgctcagatc tccttcagca cggagcgctc gagccgcgag aacgtccaga
     8821 acccgacgac gatgaacagc agggacatcg ccacgctcac cccgacgtcg acccaggaga
     8881 ccgatcgctc gaacaggccg ccacggtaga gggacaggat gccgttgagg gggttgagga
     8941 cgagcaccgc ccgcagctga tcgggcaccg agtgcaggcc gtagagcacc ggggagaggt
     9001 agaagaagaa gcgcaggaag atgcgcacga cgcgctgcat gtcggtggcg agcacggtga
     9061 tcggggcgag gatgagcccc agccccacga gcagcaggaa ctggagtccg acgccgaggg
     9121 ggaacaacag cagttcccag tcgaggtcca ccctgccctt cacgaggtag tagatgacga
     9181 agccgacgag gatgggcaag gacaggacgt actccacccc cttggcgatg acgcaccgga
     9241 tgacccagac ctctcgggga aggttcgtcg agcgaacgag gcgggcctcc tggatgaggc
     9301 tcttcgtcgt gtccgtcacc gccccggtga accactgcca cgcgagcagg ccggagagca
     9361 ggtagaggaa gtacggggtg tcggcgaccc ggccggcctt gaagatgaag gtgaagacga
     9421 cgaagtagac actcgccatg agcagcgggt cgagcacggt ccacacgtat ccgagcacgc
     9481 tgcgcgagta gcgcacccgc aggtcgcgct ggacgagggt cccgaggatc tcgcgccgct
     9541 gccacaccgc cgtgatccgc ttgatcaacg cggggacctc gattcggcgt cggcggggaa
     9601 cgaccagcgc ccgtgcaggc gccgtcgcga ggatacggca ccacgacccg cccgcggacg
     9661 aggcgacatc agatgtcgcg ttccagcacg aggatgccgt ccacgtcgcg cagcatccca
     9721 tcctggatca gcgtcgcgcg cagacgtgcg tactcgccgg ggtcgccgtg cgcgaagtgc
     9781 gccaggcggg tgaggaagag cgccacggag cggaaggacc gggtgatcac gaggttgggc
     9841 gtaccggtcg tgcagttgaa ctgcgtcacc ggggtacgcc agtccccgta gttctccgtg
     9901 aggtagcggt cggcatccgc ggggccgagc accggcaccc cggcgagcgt gtagtcggcg
     9961 aggtcgaagg cgctgttctc ccagcggtgg atcgaggacc cgtgccacag ccgatcgccc
    10021 tcgaggtggt gcacgaagac gtcgacgttg atgccgttct cgtggatgag cttgacgagc
    10081 gcaggcgcgc gctcgaccgt gaaccgcccc ggctcgacct ccacgacggt ctgcgcgtcg
    10141 tcgagcttct tgacgacgta gcgcggggag cgccgcaagg ccgcgacgag gtcatcgagg
    10201 acctccgggt gctgcgggtc gaaggtgacc ccgacatcga tgtcgtagtc gtgggcgagg
    10261 aacccaccct cgcggacgat gccgaggaag gtccccgaga tgacgtacca gcgccactgc
    10321 tggaccggga gggcatccgc gaggtccacc agggccgtgc gcgcgtcatc gacggagaag
    10381 tcaccctctc ggtcggatgc cggcgccgac ggcccaccct gggcggagct gatgagcgcc
    10441 aacaggtcgt cggcgtgcgc ccggaagtcc tcgcgcaacg cgccgatgtc ccgggcgccg
    10501 gcatccaact gggcatggat gcgagccgcc cgcacgaaga ggtcgtcagc ccgccacagg
    10561 tgctcgggca acgcgtcacg acggtgcgcg atgaacgcct cgagcgactc gaggtcggcc
    10621 ggggtcgccc cgagcgtgaa gtcgaagaag tcgtcggtca ccgtctcgac ctggggccac
    10681 ccgcgacgca gtacccaccc cgccagacgg aacgcgtact tcgtctcgtc ctccacgaac
    10741 tgggtgcgca cgagccgggc gatcgagggg cgccccgtca cggcgagggt cccggcatac
    10801 gccgctcccc cgatgccgta ccggaacgcg ggtgagtgcg accggttgag gaagttgagc
    10861 agcgagtacg acttggtgag catcagctgc cgtccgtgag gagctggtcg acgaagcgcg
    10921 cggcggcgtc gccgtcctcc agcgaggcga acttctgccg gaaggcctcg taccgcccgg
    10981 cgtgctcggc gaccacccgg tcgaggtcac cgagggcgtc gatgacgtcg ttggtgtggg
    11041 tgagcagcgg tcccggggcc tcggcctcga agtcgatgta gaacccccgc aggtcatcgc
    11101 ggtacgcctc gaggtcgtgc gtgaagaaga gcatcggccg tccggtgttg acgaagtcga
    11161 acatgaccga ggagtagtcg gtgacgagca cgtcggcgac gaggtagagg tcactgatgt
    11221 cggggtagtg ggacacgttg cgcgcgaagt caccgaacat cgaggtgtcg acgctgttgg
    11281 cgacgagctg gtgcccgcgg atcagcagga cggcgtcgtc ggcgaactcg cggtgcatcc
    11341 gctcgaggtc gagcttcatc gcgaactggt agcggcctga cctgtcgtac tggttgtccc
    11401 gccacgtggg ggcatagagg atgacccgct tgccgggctc gatgccgagc cgggctgcgg
    11461 cctgcgccgt gcgggcggca cgttcctgcg gccggtggaa gacgtcgttg cgtgggtagc
    11521 cggtctcgag cacccgtccg tcgaagcgga atgcccggcg gaagatctgc gtggagtagg
    11581 cgttcgggga gacgagcgcg tcccaggtgg ccacctcctt ggcgaactgc gtgaggtagt
    11641 tcttgttcgc catctgcagg ttctcgatgt cgaagccgat ccgcttcagc ggggttccgt
    11701 gccaggtctg gccgtagacc gtgccggggc gcttcacgta gtgcttgggc atcgagtcgt
    11761 tgctgaccac ccagcgtgcg cgaccgaggg tgtggaagta ctcgcggctg ccgacgacga
    11821 ccgtctcgac accatcgggc acctcgacgc cgtggtgctc gaccgcccac acctgacggc
    11881 gcccgtcccc acgccgcacg agctcctcga agatggcacg gggattgtcc gagtactgcc
    11941 gccccttcca cgcctcgaag aagaccgtgt ccgtcagtgg gagccgccgc gcgagtcggt
    12001 agtgcactcg gcgggcgatc ttctggttga acgcgccgcg gtccctccac gccccgcccc
    12061 cgtcgacgac gagccccagc tcgtgggacg gggaggtgcg caacacgacg acgaccttct
    12121 cgccgcccgt gtcggacagg tgcgcctcgg caccagccga gacgtgcacc ccgagcgtcg
    12181 cctcgtcatc cccgtgcacc ccggcgagga cgctccagcg tccgggacgc agccagcgca
    12241 ggcagggctg ccccggcgaa cccgtggccg gcacgagtgc gcgccacgtc ccaccgagca
    12301 cctcgacggg cacctcgaga cggtccgagc gagagagcag gacgagctga tcgagatcga
    12361 cgtcctcggt gtctccgtgc agctccagcc catccggcgt gaaggcgaag tcggtgagca
    12421 gtggtcgcag cgggccgtcg acgaggaccg ccccggtgac gccgcgctca cgcagctgca
    12481 gcagtcggcc gtgcagccgg gcccgtccgg cgcccgcctc ggggtcgacg tcgagcgggc
    12541 cgacgtggcg accgttgatc cgcaggctcc ggggggcttc ccgttcggtc gaggaggcca
    12601 ccgtgacgag ctcgtcgacg acggcggacg cgtcctgcgg gtcgtccgcc gcggccacgg
    12661 ccagccacag acgccagtgg cgttcggcga gcgagcgcga ggtgacgtgg agcgattcgg
    12721 cggggatgcg cgcggtcccc gtcgcacctt cccggtcgac gcggaccgtg acaccttcgg
    12781 aggcgtcgag gcgacgcagg tgcacgtagg agtcaggcag ctgggtgccc ggggccgcgc
    12841 gcagggtcag caccacctcc tcgccctcga ccacggcgtc gcgaacgacg accggagccc
    12901 tgcgggcggc gatccgcatc ttcttgcccc ggtagccggg aacgacgagg acgccatcgg
    12961 gcgcccaacc gcgcggcggg tgcagggcac gggagtactc ggggctgccg acgaccgacc
    13021 cccgtcgagc tgctggggcg gccacctggg cgacgagttc gtactcgacg gcatccttgc
    13081 cgtcgggcag cacgaggtcg gctgcgggga cccgggcaag gaagcccgaa ccgtcgtagt
    13141 tgaccgcccg gttcctggcc cggccggtga tgtccacccg gcgcacccga cgcgcccgca
    13201 cggaacggcg ctcggccttg gagcccacga ctcgcagctg catccggtgc accgtcgtcc
    13261 ccgggccgat gtccgggacc ccatcgatgt agccgtgccc gtcgatctcg agggaggagc
    13321 ccttccatcg cacgtcacgg atgccggtca cgagccgctg cgagcgggtc acctcgtaga
    13381 ccgagtccgg gacacccacg gacgagtccc cgaagtaggg cagctgcacg tacaggcgga
    13441 ctccgcgacg gacgacctcg ggcctgggac cgcgcacctg cagctggtgg gcctccacga
    13501 gctcggcggt gcggttctcc cggatgaggt ggtacgaaag gcggcgccgc ggtgggagct
    13561 tggcgatcac ggccgggtcg acgtcggcga ggtactcacg aaagacccgc acgagggtct
    13621 cggcgaactc ctcgtcgccc tcgtgcagga cgtcgaggaa gagcggcatg tcgagggtga
    13681 ggaccttctc gtcgtgcagc cgcttgccgt cgacctcgcc cgtgcgctcg aggaagctgt
    13741 tgaccgagga gaccgcagcc atccggtcga tgaggttgcg gctctcggcg cgacgctggg
    13801 tgatcgactg ctcggcggtc tggcgctcgc gccagaggta gaccggctcc tcgatgaggt
    13861 cgactgaccg ggccaggaag tgcgctggca ccgtcaaggg gatgtcctcg tagaggacgc
    13921 cctcggggaa gcgcagcccg tgctcgatga ggaactctcg ccggaagatc ttgttccacg
    13981 cggtggtgtc gtacagcagc gagggcatgg cccgcacgtg cgtgccgatg cgggtctcgg
    14041 ggaccgcccg gcggtgcagt ccggacgggc gggtacgggc tccgtcgtag cggagcacac
    14101 cgccgctgac gatgtcactg ccactgcctt cgatggcgtg catcatcaac tcgtaggcat
    14161 cacgtgggac gacgtcgtcg gagtccacga acgtcacgaa ctcggtgccc gcagcgtcga
    14221 gtccgatgtt gcgcgcccgc cccaggcctc cgttctcgac gtgcaggacc cgccaccccg
    14281 gacgtccctg cgcgaactcg tcggcaatct cccccgatcg gtcgggggat ccgtcgtcca
    14341 cgagcaggac ctcgaggtcg ctgaaggtct ggtcggccag ggactccagg cacgacacga
    14401 ggtagtcctc gacgttgtag atcgggacca ccacactcag ccgcggacgg gaaggggctg
    14461 ctgggtggtc tcgcacagtc gttcagtatt ccaaagtgag tcgcggttgc gggaatcgtg
    14521 gcgagtcgcc gccacggcgc cgaggcctgc tcgcaccgat cacggcgtca gcctgccgag
    14581 cagacgctcg cgaggtccga ggtgtcggca tccgggtccg aaccgggacg atccatgacc
    14641 gcctgcttct tcggttccgt cgcctgcgcc ttcgcgccac caccggatgc cgtggacgcc
    14701 tccggccccg cggcacgctc ctcggccgcc ttctcgtcgg cctgttcgga ccgtgcgatg
    14761 gccttgtcga ccgcagcgtg cacgacggcc gggtcgtagt cccacggctt gatgagcgga
    14821 ggcacgaagt tcaccgaggt gatcttctgc tgcttggtct tcacggccag gtcacccagg
    14881 gtcgcgagcg cgccctgcgg gatgtcggtc tggaagacgc ccttggtggc gttggcgatg
    14941 tcgccgaagt tcaggaccac ggtctgcggg tcgagctggc ggatcatcgc ggtcacgaca
    15001 caacgctggc gggccatccg ctcgtagttc gtggagttgg cccggctccg ggcgtaccag
    15061 agcgcgtcgt ggccgtcgag gtgccgctcc cccgcctcga tccagccctt gatgttggcc
    15121 tgcgagccga tcggcgtgcg tcgctgcacg gtgatgtcca ggccacccac ggcatcgacg
    15181 aggctgcgga accccttgag gtcgacgagc acgtggtact ggatgtcgag gccggacagc
    15241 gcctcgaccg cctcggtcgt cgcgagcagg ccggggtcct tcgtgccggc cgggaactca
    15301 tcggcgtggt cacgccccca ggtgtacagg ccgttgagga ggcattcgtc accgcaggtc
    15361 cacccctgcg gcatcagccc agccatgacc gagccgggtc gaaacgtgat gtgctccgtc
    15421 tcgcgggaga agccgaacag cacggcccgt cccgtctcgg catcgacgct ggccagctgg
    15481 atgctgtcgg gtcgcaggcc ctcacgcccg cgaccgctgt ccccgccgag caggaggatg
    15541 ttgaaccggc cgtcgacggc tccggccgcg tccccggcac cgaacaccga ggtcatcgcc
    15601 gtgcgggcag cccccacgtt gacgccggcg taggcggtgc cccccggaag gatgacgagc
    15661 aggacgacga gggcgacgag caggcgacgc cggtcggttc gagcgagccg gtcggggcga
    15721 ccgatccgcc aggcgtcgac gaggagcccc agccacagcg cggccatggc gaagagcacc
    15781 acctggagca ccgcgaggaa ccaccggtgg gtgatcaccg tgagcgccgc cgtgcggttg
    15841 acgactgcca ggaggccgag gagggcggca cccgcccaca ccccgaacca gcccctgagg
    15901 gcgaagcgcc cgagggtcgg gttgccgacc gcgagctggg ccgagccggg cagcacgacc
    15961 gtgaggccga gcagcgcgta ggcgcggcgg cggtcgcggc gcgggtcgcc cgtggtgcgg
    16021 gtgtcaactc ggatcggcgc ttcggtcgcg gtgcgatcga gcaccgcgga gccgctctgt
    16081 gatcccatga ctgcctacta tcaggcctgt cgtcgccgcg gcgaccgagg cgcgccgccg
    16141 tcgggagcat tttgcgatca ggcagatccc agactccgtt ggcagactgg tgccgacgtg
    16201 accccccagc ccgagagcac tccacgacat gaacgacgac cacgcacggc cgatccccca
    16261 gggcaggcgc ccagggagac cggccgacga ggacgtctac gtcgtcgaca cccgacgggc
    16321 cagccacccc gccccgacct cgacagcaaa gcctcgacgc gcagccacgg ccccgaaacc
    16381 gggccacacg gcatacgacg aactcggtcg gcccgtctcg cccggccgac cgacgccccc
    16441 gaccccgccg cgcgggcctc gcaccccgcg ctcgacccga ccgccccggt ccggacgggc
    16501 gcggcgcctg tcgcggctgg tcctggcggc ggtcgcggcc tgggtggtct tcatcgtggt
    16561 caccccgatc cacgcgtggt cgcaggttgc ccgtgaggac accactccaa cgacgcagcg
    16621 gccggcggac gccagcggaa gcacctacct gctcgtcggc tcggacagcc gcgaggggct
    16681 cactgctgct gaacgcaagg cgatcggtgt gggcggtgac gccgaggggc gccgcaccga
    16741 ctcgatcatc ctcgtgcaca cgccgagcgg gtcgggcaaa ccggtgctca tctccatccc
    16801 ccgcgacagc ttcctgccga tccccgggca caaggacaac aaggtcaacg cggcgttcgc
    16861 gatcgggggc ccgagcctgc tcgtcgccac gctggaggag gccacgggtc tgcccatcga
    16921 cggctacgtc gagatcgggc tcggcggatt cgccggcgtg gtcgacagcc tcgggggcgt
    16981 cgaggtgtgc gtgaagaacg acatcaagga tgccaaggcc cacatcgacc tcaaggccgg
    17041 ctgccaggag ctggacggca agaacgcgtt gggttacgtc cgagcgcgct actccgaccc
    17101 tcgcggcgac ctcggccggg cggaacggca gcgtcagttc ctcggcgccg tgatgaagca
    17161 ggccgcgaca ccgtcgacgg tgctcctccc ctggcgctgg tggggcttca cccacacggc
    17221 gacgtcaagc gtcgtcgtgg gcgaggacac ctcgctgctg gactcctacc ggatcctgtc
    17281 gacgatgcga aaggtctcgt ccgaccaggc cctgagcctc gtcgtgccgc tgtcgtcgac
    17341 gaacacgacg acgagggccg gcagctcggt catctgggac gacgagcgcg ccggtgccct
    17401 gttcaccatg ctgcgcgagg gcacaccgat cgacagcccg cccgagggca ccgacggtcg
    17461 gccacgaggc tgacccccga cgccctcccg cgacgacgag ctggccggct cagcccttca
    17521 ggagctgacg ggccatcacg atccgctgga cctggttggt gccctcgtag atctgggtga
    17581 tcttcgcgtc gcgcatcatc cgctcgaggg ggaagtcgcg ggtgtacccc gcgccgccga
    17641 gcagctggac ggcatccgtg gtcaccttca tggcgacgtc ggaggcgaag cacttggctg
    17701 ccgcaccgaa gaacggcagg tcggcgtccc cgcgctcgga cttcgcggcg gccacgtaga
    17761 ccatctggcg ggcggcctcg agctccatgg ccatgtcggc gagcatgaac tgcacgccct
    17821 ggaactcggc caccgccttg ccgaactggc gacgctccct gacgtaggcc acggcctggt
    17881 cgagcgcccc ctgggcgatg cccacggcct gggcgccgat cgtgacgcgg gtgtggtcca
    17941 gcgtggccag tgcgagcttg agcccgccgc cgacggtacc gacgacccgg tcacccggga
    18001 tgcgggtgcg gtcgaagtgc agctcacggg tgggcgagcc cttgatgccg agcttgcgct
    18061 ccttctcccc gaaggtgaac cccgggtcgt ccttctccac gacgaacgcg gtgatgttgt
    18121 tgccgcgcgg gccgtccggg tcggtcacgg cgagcaccgt gtagaactgc gagacccccg
    18181 cgttggtgat ccacgacttc tgccccgtga gcacccagtc gtcgccgtcg cgcaccgcct
    18241 tgcaccgcat cgccgcggtg tccgagccgg cctcccgctc ggacaacccg taggagaacg
    18301 tcgtctcccc cgccgcgagc ggagtgaggt agcgggtctt caggtcctcg ctgcccgcga
    18361 ggatgacggg catcgacccg agcttgttga ccgccgggat gagcgaggcg gaggcatcgg
    18421 cccgggcgac ctcctcgatg acgatgcagg tcgcgagcgc gtcggcaccc acgccgccgt
    18481 actcctcccc cacgtgcggc gcgaagaagt cgctcgcgac gagggcgtcg tgcgcctcct
    18541 gggggtagcg cgcctcctcg tcgaccgcgg ccgcccacgg ggcgatcttg tcctgcgcga
    18601 cggcgcggac ggcctcacga atcgcctcgt ggtcctcgga gagccggtag gtgtcgaagt
    18661 cggggttgcc actcatgaca ggcatggtat cgatcggtat accgatggcc gtccggggtc
    18721 gcgtgtctca cctgaccggc atctcaacga ccgtggagga agtgccacat cccgccgtgc
    18781 tgctcgcgct ccatgaacgc ggggatgacg gcctgctcga acagctccac acccgacagg
    18841 tcgtacgcct gctcgacgaa gtacgtgatg gcgtacgtca tcccgcgcga gcgcaggtcc
    18901 accagcgcct cgattatctg ctcgggtgtg ccgacgagcg gaccgtcggc gaacgacttc
    18961 acggtcgact cgacgacgtc ctccggcagg cccgccgagg tgtagtggtc acggatccag
    19021 gccagccgct cctgcacctc cgcgtcgtcg cggccgatga cgacgttgaa gttggcgctg
    19081 cgcacgatgt cctcgaactc gcgccccacg tcagcgcagt gcgccctgag gatctcgctc
    19141 ttggcgctga actcctccgg catccccgag aagttcgtgt agtcggcgta ctccgcggcg
    19201 atccgcagcg tcttcttctc gcctccgccg gcgatccaca cggggatgcc gttgcgcggg
    19261 ctccccggcg cggaatcgcc ctgcagcggc cgggggaagc acatggcccc gtcgacggtg
    19321 aagtgttccc cggcgtagtt cccgtagccg gtcgtccaca tctgctgcat gatctcgacg
    19381 ccctcgcgca gcgcctgcag ccgctccccc gcgctcggga agccgtagcc gtaggcgcgc
    19441 cactcgtgct cgtaccagcc ggcgccgatg cccatctcga ggcgtccctc cgagatgacg
    19501 tcgaccgtcg ccgcgacctt cgcgaggtag gccgggttgc ggtaccccat gcacgtgcac
    19561 atctggccca gtcgcacccg acccgtgacc ccggcgaagg cggccatgag ggaccaggcc
    19621 tcgtgcgtcg tctcctcgat cggacgggga acggtgtgga cgtggtcgta gacccagatc
    19681 gactcccagt cggggttgcc gtcgacccgc cgggcgagac cagccatcac gccccagtgc
    19741 tgcgccgggt cgatgccgac gagatcctga cgccacccct gcgggatgaa caggccgaac
    19801 ttcatggtga tcctctcgag ggcagtgtca cgagcgcacg cccccagcca actccgccgc
    19861 ggtcgtcgac gcaactcgcc gccccgtatc gtggggcgac atgagccgaa ccatctgcgt
    19921 cgtcgggacg tcctacgtcg gtctgtccat cgccgtcctg ctcgcggagc accacgacgt
    19981 cgtcgccttc gacatcgacg agagccgtct ggagcagatc agggcgggac gcagccccat
    20041 cgccgacccc gacatcgagg agcgcctggc ccgctccgac ctgcggctca cggtgaccaa
    20101 cgacaagcac gaggcctact cgcgcgccga gtacgtcgtc gtcgccacgc cgacgaacta
    20161 cgacgagcgc accaactact tcgacacgag cagcgtcgag caggtcgtct ccgacgtcgt
    20221 caccatcaac cccgacgcca ccgccgtcat caagtcgacg atcccggtcg gcttcacggc
    20281 cggcctgcgc cgacgcctcg gcaccgacaa catcatcttc tcgccggagt tcctgcgtga
    20341 gggccgggcg ctgcacgaca acctgcaccc gtcacggatc gtcgtcggcg accagagccc
    20401 acggggtgag cggttcgccc agctgctcgc cgaggcagcg ctcgacgacg acgtgccggt
    20461 actgctcacc gacagcaccg aggcggaggc ggtcaagctc ttcgccaaca cctacctcgc
    20521 gatgcgggtg gcgttcttca acgagctcga cacctacgcc gcaacgcacg gcctggacac
    20581 ccgccagatc atcgagggcg tcggcctcga cccgcggatc gggacgcact acaacaaccc
    20641 gtcgttcggc tacggcgggt actgcctgcc caaggacacc aagcagctgc tcgcgaacta
    20701 cgccgacgtc ccccagacac tgatcagcgc catcgtcacg gcgaacacga cgcgcaagga
    20761 ctacgtggcc gccgacatcg tcgcgcgggc gccccgagtc gtcggcatcc accgcctgat
    20821 catgaaggca gggtcggaca acttccgctc cagcagcgtc cagggcgtca tgaagcggat
    20881 caaggccaag ggcatcgagg tgatcgtgta cgagcccgag ctgagcgacg accgcttcta
    20941 cggctcggag gtcgtccgcg acctcgacga gctcaagagc cgcagcgacg tcatcgtcgc
    21001 caaccgcctc gtcgacgagc tgcgcgacgt ggccgacaag gtctacacgc gcgacctgtt
    21061 cggcaacgac tgaccggggc atgatgtcgg catgggcctg aagcaccaga acgaccccga
    21121 cctcgtccgc gggctgctca tggacccggg aacctgggtc atcgtggggt tgtccgccaa
    21181 ccgtgaccgc gtcgcgtacg ggatctccca gtggttgcac gtcgagctcg gcaagtcgat
    21241 catccccgtg caccccaagg ccgagaccgt gcacggcgcc cggggttacg cctcgatcgc
    21301 cgacatccct gaccaggacg tcaaggtcat cgactgcttc gtcagctcag aacacgtcgg
    21361 ggcggtcgtc gacgaggcga tcgcgcacaa ggaccgcctg cggatcgatg ccgtgtggat
    21421 gcaggtcggc gtggtcgacg cggctgccgc cgagcgcgca cgggccgctg gcctcgacgt
    21481 cgtgatggac acgtgcccca ggcacgagtg gcccaaggtg cgcagcgagg gcatgcagcg
    21541 ctgacgcgtc gtcagtcgcg gatgccgtcg gcgcgccgtt cgcgcagcgc cgcacccttc
    21601 gcgtgcgcct cgtcacgaag tgcgccccgg aaggtcgtga gggcgtcgcg gatccgcgcg
    21661 gcctcaggcc cctcaccggc accgaggatg cgcgcggcga gcaggccggc gttgcgcgcc
    21721 ccgccgacgg agacggtcgc gaccggcacc cccgccggca tctgcacgat ggacagcagg
    21781 gaatccatgc cgtcgaggta cttcagcggc acgggcaccc cgatgaccgg gagcgaggtg
    21841 accgacgcca gcatgcccgg caggtgggcc gctcccccgg ctccggcgat gatgacgcgc
    21901 agaccgcgct cctgcgcgct ggcgccgtac cccaccatct cctgcggcat ccggtgcgcc
    21961 gagacgacgt cgacctcgca ggcgatgccg aactcgtcga gggcagcggc cgccgcctgc
    22021 atgaccggcc agtccgagtc cgaccccatg acgacgccga ccacgggcac ctgaccggtg
    22081 acctcggcgc cctgttcctg tcggctcatt cggtcaccac tccctggatg tagtcggcgg
    22141 cgtgggcggc gcgctcacgc aggtcggcga ggtcgtcgcc gctgatgttg acgtgcccga
    22201 tcttgcgccc cgggcgaact cccttgccgt acatgtgcac cttcgcctcg gggtccctcg
    22261 ccatgacgtg ccggtacgtg gggtacaggt ccgggtagtc accgccgagg acgtttgcca
    22321 tgacggtcca gcgctcgtgc ggtcggggcg agccgagcgg gaggtcgagc acggcccgca
    22381 ggtgctgctc gaactgcccg gtgaccgcgc cgtccatcga ccagtgaccg gagttgtggg
    22441 ggcgcatcgc gagctcgttg acccggaacg ccggcccacc ggcatccacc acctcgaaca
    22501 tctcgaccgc cagcaccccg gtgacgtcga gctcgccggc gaggcgcagc gcggcgtgga
    22561 ccgcgtcggc tgcgaggtcg gcgtccagcc ccggggccgg ggcgagcacc tcggtgcaga
    22621 tgccgtcggt ctggaccgtc tcgacgaccg gccacgccgc ggcctgcccc gaagggctgc
    22681 gggcgacgag cacggccagc tcccgcacga agggcacccg ctcctcgagg agcagcccgg
    22741 tgccgtccgt cgcagcccgc tcgagccagt cggcgaggtc gtcggcggat gccgcgagcc
    22801 gcaccccctt gccgtcgtag ccaccccgcg gggtcttggc gacgaccggc cagcccacgg
    22861 catccccgaa ggcggtgacc tcggcgacgt cgtcggcgcg cgtccagcga gggcacggga
    22921 tgcccagctc ggtcagccgc tcacgcatcg cgagcttgtc ctgcgcgaaa cgcagggcag
    22981 ccggccgcgg gtgcagctcg accccctcgg cgacgaggag gtccaggacg tgcgcgggga
    23041 cgtgctcgtg gtcgaaggtc acgacgtcgc agtgctgggc gaacgcgtgc accgcctcgg
    23101 ccacggtgtg gtcaccgacc ggcgagctgg gcacgacgag ggcggcgctg gcgtcctcgg
    23161 cctcggcgaa cacgctgagc gtgatgccga gctcggcggc cggcccggca cacatccgag
    23221 cgagctggcc acctccgatg atgccgacga cggggaatcc tccgggagcg cgacgtgggg
    23281 tggtcaccca gcgagaatag cgacctctct tggttaggct gcatcggtgt cccatccccc
    23341 ctccacgccg acgcgcggac ctgcgcgact catggcctgg gcccgaggcg cgatggacgt
    23401 catctaccgg gagatgatca agttcggtgt catcggggcg ctcgccttcg tcatcgacct
    23461 cgggctggcc aacctgctgt ggcacacggt cctcgaggac cgggtcacca ccgccaagat
    23521 catctccggc ctcgtcgcga ccctgttctc gtgggtcggc aaccggcagt ggaccttccg
    23581 gcaccgccgc agccgcccgg cccaccacga ggtggccctg ttcttcggcg tgaacatcgt
    23641 cgccctcggg atcgccacgg tcaccctggc gttctcgcac tacgggctgg gcttcacctc
    23701 acgcctggcc gacaacgtcg cgacgatcgt ggggatcggc ctcggcacgc tgttccggtt
    23761 ctggacctac cgccgcttcg tcttcgccag cgagaagatc tgactcagtc ctcgctgacc
    23821 gacaggaaga gggcgaacaa cggcggcacg gcacgcgcga gctcgagccg tccccccgca
    23881 gcctcggcca ggtcccgagc gaggctgagc cccaacccac tacccgagga cgacaccgct
    23941 cgctcgaaga tgtgcggcgc cagggtcggc gcgacacccg cgccggtgtc gctcacctcg
    24001 atgacgacgg acgggccgga ccgacgagcc gccacctcga cggtgcccgc gccgtgggcc
    24061 agcgagttct cgagcagggt cgtgaggatc tgcgccaacg ccaccggggt cgcccgcacc
    24121 cgcagacccc gctcaccgct cacgtggatg ctgcggcggg ccgcggcgaa ggccggctgc
    24181 cactcgcgct ggagcgaggc gaggaccgag tcgagggaga ccgccgggtt ggtctcgacc
    24241 ccgctgcggg ttcggctcat caggtcgtcg acgactcggc tcagtcgttc gacctgaccg
    24301 atggcgatcg tggcttcctc ccccacgacc gtgaggtcgt cggtgctcgc gatctcctcg
    24361 agccgcatga gcaacgcggt cagcggcgtg cgcagctggt gggaggcgtc cgccgcgaag
    24421 tcacgctcgg aggcgagccg tttcgcaccc tgctgcgccc cccgtgccag cgccgcatcg
    24481 aggcgatcca cctcctcgat ccccgagttc agcgggaccg ggcgcggatc ccccggtgcc
    24541 agacgctcgg cccgctcccc cagcgtccgc aggaagtgcg agacccgcag ggcccgacgg
    24601 tccgcgaccc acgcggcgac ggcgagcgcc agggcggtcg cgacgagaag ggcgaccagg
    24661 tgggcccaga gcgagagcag gggtgaggag gcctcacgtg ctgtttgctg ccctgcggag
    24721 atgatcgact cgctggggtc gtcacgccac aggatgaaga tcgagaaccc cacggtgatg
    24781 atgaccccgc tgatggcgac cgcctgcacg gctgtccaga cgagcaggcg acgcacgagg
    24841 gctcactcct gcggggcgtc gaagcggaag ccgaccccgc ggatcgtcgt gatgtagctg
    24901 ggctgcgacg cgtcgtcgcc gagcttcttg cgcagcaccg agacgtgcat gtcgagcgtc
    24961 ttggtggagg tgtaccaagc gctgtcccag acctcgcgca tgatgcgctc acgcgagacg
    25021 accttgccct cgttggcgac gaggagcttg aggagctcga actccttgcc cgtgaagggc
    25081 acctcctggc cgcgcaggaa gacgcgccgc ccctcggggt cgatgcgcac cagcgagtcg
    25141 gcggccggtt cgacgacccc acggcgcagc agcgcccggg tacgcgccag gagctcggcc
    25201 agccggaacg gtttggtcac gtagtcgtcg gccccggcgt ccaacccgac gacggtgtcg
    25261 acctcgtcgg cgcgcgcggt cagcacgagc acggggacgg tgcccccctg ggcgcgcagc
    25321 cggcgacaca cctcgagccc gtccatcttg ggcaggccga ggtcgagcac gaccaggtcc
    25381 ggtgccaaca acgcctgcgc gagtgcggac tccccgtcgg tgaccacgtc cacctcgtag
    25441 ccctcgcgcc gcagcgcccg ggccagcggc tcggagatcg cggggtcatc ctccgcgagc
    25501 agcaccttgg tcatggcttc gatggtagtc gcgcgtcgat ctcccggagc gctggacctc
    25561 gaccgcccgg ggcgcgctgg agctcgtgga gggtcaccac gccgatcccg cgcagcatcc
    25621 gacggcgacg ctcggtggcg ctgaagcccg acaccgagcg caggaccttg gccatgtcct
    25681 catcgacgaa gacgtggccg gagggggtga cggcggtgat ccggctcgcc ttgttgaccg
    25741 tcagaccgaa gacgtcaccg aggcgggaga ccacccggcc gtgggccagt ccgacgcgca
    25801 cgggcggcag cagctcgtcc tcggccatgg cgtccacgag gtcgagggcg atcgccgagg
    25861 cggccgcggc atccgtgtgc acgaagagga cctcgtcgcc gacggtcttg atcacccgcc
    25921 caccgtgctc ggtgatcacg tcactggcca acagctcgaa ccgctggacc agccgggcca
    25981 gctgccgctc gctcatccgg cggacgaacg aggtgaagga caccaggtcg gcgaagccga
    26041 cgacccggat gggggcggac gggtcggagt gcacggccgg gtctgcgtcg gcgatcatgc
    26101 gggcgatcgc gttggtcagg tgacggcgcc agacgtagac gagcaggggc tcgatgtcgt
    26161 cacagatgtt cgcgagcagc tcggcggcat cggcagcgat gcgcggctcg ggcaccgaac
    26221 ggctgtcctt gccctcgagc tcctcggaga acggcgacgt gagcgactcg gccacgagct
    26281 gggtctgcca gaccgcgagc cggtcggacg tgcgggcgaa ggcacgcgtc atgcccaggg
    26341 cgagctcttc actgacctcc ccgtcgccga tgaggcgggc cacccgtttg agggcctcga
    26401 ggtcggcctc ggtgaacatc gcgtcctcgt cgtcgacgtt ctggaacccc atcgcgtgcc
    26461 agaagcggcg cgccaccacc ggttcgaccc cggcgccctc ggagacctcg cgacggccca
    26521 tggaggccgg ccggccgagc agtcgccgct cgatctgccc gggcagctcc tcgacgaggg
    26581 ggtggtcgcg ggcgccgctg acctcctcgg tggtcatgga actcctctca gcgcgacctg
    26641 acgtggacga cgtcaccggc ggacacggac tgggtgccgg actcgtcccg gaccgtcagt
    26701 ctgcccgctg cgtcaactcc tgtcccgatc ccgtggacga cggcgccccc gggcaggtgg
    26761 acctcgacga cgcggcccac ggtcgagcag gccgccacgt acgccgcctg cgcgctgccg
    26821 ccggggccgg tgtcgtcgcg cagcagcgcg gccaggtggg tgaggaaggc cgagagcagc
    26881 gcggcgcggt ccacgccggg ggcgcccgcc gctcgcagcg acgtggccgt gtcgagcggg
    26941 aggtcctcgc gggcggtgtc gacgttgatg cccaggccca cgaccacgcc gtcggccatc
    27001 cactcgcaca gcaggccggc gagcttgcgg tccccgtcgc tcgggacgag cacgtcgttc
    27061 ggccacttca gcgccgtctc gaggccggcc acctcggcca cggcccggcg aacggcgaga
    27121 cccgcgagca gcggcaccca cgacgggccg gatgccgggg gtggcacgaa cacggacacg
    27181 gccagggagg tgcccggcgt cgtcgtccac gtccgcccga gacgcccgcg accggcatcc
    27241 tgctcctccg cgacgaccac gcgccacggc gagggagccg atcgaacctc gtcgttcgtc
    27301 gaccccaggc ggtcgaaaac ctcgatgggc ggccacggcg cgcccggtgt gacggatcgc
    27361 tcgctgagcg gtcgctggtc agggttctgg ctcacgctcc caaggctagg ctcgggccat
    27421 gccctccagc gacaccaaag ccatcggcgg taccgacgtc ccgaaggaca tcgacaccac
    27481 gacgaccgcc ggaaaactcg ccgacctcaa gcgccgcgtg gccgaggtcg cgggtgccgg
    27541 atccgagcgc gccgtcgaga agcagcacgc ccgcggcaag aagacggccc gcgaacggct
    27601 cgagctgttg ctcgacgagg ggtcgttcgt cgagatggac aagtacgccc gacaccgctc
    27661 ggtcgcgttc ggccaggacc gcaaccgccc ctacggcgac ggcgtcgtca ccgggtacgg
    27721 caccgtcgac ggccgcaccg tcgccgtgtt cgcccaggac ttcaccgtct tcggcgggtc
    27781 gctgggtgag gtcttcggcg agaagatcac gaaggtcatg gacttcgcga tgaaggtcgg
    27841 gtgccccgtc gtgggcctca acgactcggg tggggcgcgc atccaggagg gcgtcgtctc
    27901 cctcggcctc tacggcgaga tcttccggcg caacgtgcac gcctccgggg tcatccccca
    27961 gatctcgctc atcatgggcc cgtgcgccgg cggcgcggtc tactcccccg cggtcaccga
    28021 cttcaccgtc atggtcgacc agacctcgca catgttcatc accgggcccg acgtcatcaa
    28081 gaccgtcacc ggtgaggacg tcggcttcga ggagctcggc ggcgcccgca cccacaacac
    28141 gacgtccggc gtcgcccact acatgggggc cgacgaggac gacgccatcg agtacgtcaa
    28201 ggcgctgctg tcctacctgc cgagcaacaa catggagcag gcaccggtgt tcggcgagga
    28261 gaacgacctc gaggtcaccg acgacgaccg cttcctcgac acgatcatcc cggacagcgc
    28321 caacgtgccc tacgacatgc acgaggtcat cacccgagtg ctcgacgacg gcgagttcct
    28381 cgaggtccag ccgctgttcg ccccgaacct cgtcgtcggc ttcggccggg tcgagggcca
    28441 ctcggtgggc atcgtggcca acaacccgat gcagttcgcc ggcacgctcg acatcaacgc
    28501 ctccgagaag gccgcgcggt tcgtgcgcac gtgcgacgcc ttcaacgtgc cgatcctgac
    28561 cttcgtcgac gtgcccggct tcctgcccgg caccgaccag gagtgggacg ggatcatccg
    28621 ccgcggagcg aagctgatct acgcgtatgc cgaggccacc gtgccgctgg tcacggtcat
    28681 cacccgcaag gcctacggcg gcgcctacga cgtcatgggt tccaagcacc tcggcgctga
    28741 catcaacctc gcctggccga ccgcccagat cgccgtcatg ggcgcccagg gtgccgtcaa
    28801 catcctctac cgcaaggagc tggcggccgc cgccgagcgc ggggagtcgg tcgaggaggc
    28861 gcgcgccacg ttcatcaagc agtacgagga cgcgctggcc aacccgtaca tcgccgccga
    28921 gcgcgggtac atcgacaccg tcatcacccc gtcgaacacc cggatgaacg tcaccaaggc
    28981 cttgcgggcg ctgcgcacca agcgtgcgtc gctgccaccc aagaagcacg ggaacattcc
    29041 cctatgacct cttcacaggc tcagagctca tggggacccg gccctatgac ctcttcacag
    29101 gctcagagct catggggccc ggcccgatga cggcgtcaca ggtaccggat gccgttaccc
    29161 ccgtcatcgt gcggggtgcg gcgacacccg aggaggtcgc cgccctggtg gcggtgctca
    29221 gtgccgtggg cggctccagc gacggctcaa gcgctgcgga tgccgcgtcg agcgtgtctg
    29281 cctgggccac ccccgccgcg acgatgaggc gaccggtcgg gtacggccct ggagcgtggc
    29341 agaactcgct gcgttgacgc ccgtacccac atcgaccaat aggtcacccc aggtacgccc
    29401 ccaccccacc agcccgtacc cacatcgacc aataggtcac cccaggtacg cccccacccc
    29461 accagcccgt acccacatcg accaataggt cacctcaggt acgcctgtgg ataacgaaaa
    29521 cggggaaccg cggcgcggtg ccacgcttgc tggatgccgg acacgtcacc accgctgcca
    29581 gggcctttcc tgcgtgccga ggccaagaac ctcggggtct cggatcggag gctgcgaagc
    29641 ccggacttcc gcacggtctt caccggcgtg gtggtcgacg cccggatccc cgacacggtt
    29701 gtcgtgcggt cgcgcgcagc catgctggtc tgccccgaag gtggggtgct cagccactgg
    29761 accgcagcac ggttgtgggg cggcgtcgtt cccgacaacg agtggatcca cgtcgcgttc
    29821 atgcgagatg tcaggtttcg cgtcagcggg gcgaaaccgc accgtttccg gcatcgcctc
    29881 gacatcaggt tccggcacgg gctcccagtc acctcgcctg cccagacgtt ctgccacctg
    29941 gcgaggcacc tcgggttggt cgatctcgtc gctctcggag accgactcgt caagaagggg
    30001 cggtgcacgc cggatgacct catcgcctat gcgcaaaact ggccgggcca gtgccgggtg
    30061 gaggctctca gcgcggcgaa actcgtgagg aagggcgtcg actcagtccc ggagagtgcg
    30121 cttcggctcc tgatggtgct ggctgggctt cgcgagcccg aggtgaacat ccggctgcgg
    30181 gacgtcgaag gcaatgtgcg gtttcgcatc gagctcggct acgaggacgc gctgctggcg
    30241 atcgagtacg acggccgttg gcacgaggaa cccgcgcaga aggcacacga tgacgagcgc
    30301 cgatccggcc tgagggagcg cgatggttgg accttcgtca tcgtgacggc cgatgacctc
    30361 tacggactcc ccgagcaact gctggagcgg atccgcagcg ccatggagtc tctgggcatt
    30421 ccggttcccg cggtcctgtc cgacggctgg cgtcgccact tccgagtgga caccgctgct
    30481 gcgtgatgct gcccttgcga cggtgcgcga tcgattcgac cgtcccccga gtgacctatt
    30541 ggtcgatctg gcgacggccc gtgatcgatt cgaccgtccc ccgagtgacc tattggtcga
    30601 tctggcgacg gcgggcgagg ctgcgctcca ggtcgaggct gtcggccggc gcgagcacct
    30661 gcgccgcgac gacccgggcc gcgaggtcgt cgtcgcccat gaaggcgccg aacacctcgg
    30721 gcacggtgat gccgtgctcc ggcggctgcc ccacctcgat ggtgtcgccg accgagagca
    30781 caccgggttc gacgaccgcg aggtacgcac cggttcggcc tcgctcggtg aaccggcgca
    30841 cccacccccg ctcccccatc caggcggcga aggtcgcgca cgggatccgc ggtcccgtca
    30901 cctcgagcac cgccgagccc accgtccacc gctgaccgac gacagcggca tccacatcga
    30961 gtccgagggt ggtgaggttc tccccgaaca tgccgtcccg caggtcccgg ccgagctcgg
    31021 cgccccacca gtccagctcc tcgcgcgcca cggcatacac cgcctgctgt gacccaccgt
    31081 ggtgctttcg gctgacgacg tcgtctccca gcaccccgct tcccaggcca ccgcgcttcg
    31141 ggccggggtc gcgcacctcg atcgtgtcga ccgggcgctt gtcgatcgcc gtgtcgccgc
    31201 cgcggggccg gatgccgctg cgggcaccac cgatgttgag cgaagtcacc acgggtttca
    31261 tggccgagag cctatggcgg ggtctggctc ggcgcggtgg cagccagccg taccccgggt
    31321 gacctattgg tcgatgtcgg gacggggcca ggcgtacccc gggtgacctg ttggtcgacc
    31381 tcgggacggg gtgctccggt agcgtccaga gggtgaccga gccgaccgcc gagccccgta
    31441 ccaccaccgc cgtcgacgcc gtggccgacg cctacttcga cgccgccatc gtcgccagcc
    31501 cgatcgaggc cacctacctg ggtgtccccg gccgggacac cgagctcgac gacctctccc
    31561 cggacggctt cgcccaccac gcccacctcg cgcgacggac cctggccgcc ctcgacggcg
    31621 tcgagccagc ggatgccgtc gaccgggtga cggtcgccgc catgcgcgaa cgcctcggcc
    31681 tcgtcatcga gacccacgac gccggctacg acgccatggc cctcaacgtc atcgcctccc
    31741 ccctgcaggc ctgccgcgac gtcttcgacc tgatgcccac cggcacgcag gagcactggg
    31801 ccaccatcgc cgcccgactg tccgcggtgc cgcaggccat ggagcagtac acgcagaccc
    31861 ttctcgacag tgccgaccgg ggcctggtca cgccgcggcg ccaggtcgag gcctgcatcg
    31921 tccagtgcga ggagctcacc gcccccgacg gcttcttcgc caccttcgcg cagggggcca
    31981 gcgccgacgg cgcccccctc gccgactcgg tgcgggccga cctcgaccgc ggcgcccggg
    32041 ccgccgcgca ggcctacgag cagatcggca ccgcgttgcg cgagcgactg ctcgggctcg
    32101 ccccgacctc ggatgccgcg gggcgcgaac gctacggcct cgcctcgcgc tcgttcctcg
    32161 gtgccacggt ggatctcgag gagacctacg cgtgggggca ggaggagctc gcccggatca
    32221 ccgacgacat ggcacgcacc gcagaccgca tcctcccggg ggcgagtgtc cgcgaggcca
    32281 tcgcccacct cgagagcgac ccgcgctacc agctgcacgg aaccgacgcg ctgcgcgact
    32341 ggatgcagga gcgcgccgac gaggtcatcg cggcgatggc cgacacgcac ttcgacatcc
    32401 cggagccggt gcgcaccatc gagtgcctga tcgcccccac ccagacgggt ggcatctact
    32461 acaccgggcc gagcgacgac ttcagccgac cgggacggat gtggtggtcg gtccccaagg
    32521 gcgtcaccga gttcggcacc tggcgcgagc tgacgaccgt ctaccacgag ggggtcccgg
    32581 ggcaccacct ccaggtcggc cagaccgtct accgccgaga gctgctcaac aagtggcgcc
    32641 ggatgatgtg ctggacgagc gggcacggcg aggggtgggc gctctacgcg gaacggctca
    32701 tggccgagct gggcttcatg gacgacccgg gcaactacct cggcctgctc gacggccagt
    32761 cgctgcgcgc cgcccgtgtc gtcctcgaca tcggggtgca ctgcggcttc gaggccccgg
    32821 ccgaggtggg tggcgggtcg tggacgtacg acaaggcctg gaccttcctg cgggcgcact
    32881 ccaacgaggg tgaggagatg ctgcgcttcg agctgaaccg ctacctcggc tggccggggc
    32941 aggccccgag ctacaaggtc ggcgagcggc tctggctgca gctgcgcgac gaggcccagc
    33001 agcgcgccgg cgacgcgttc tcgctgaagg acttccaccg acgcgccctc gacgtcgggt
    33061 cggtggggct cgacgtgctg cgcgacgccg tgctcgactg atgggttcgg agccggccgg
    33121 ggacgggcgc ggggccgtgg cccgggggcg ccggcccgcg ggcagcggca cccgcgaggc
    33181 catcctcgcg gccgcggccc gcgccttcgc cgagcagggc tacccccgaa cgacgttgcg
    33241 cggcatcgcc aaggacgccg gggtcgacac ccgcctcgtg tcgcactact tcggctccaa
    33301 gcaggagctc ttcgtcagcg tggtcgagct ccccttcgac cctgacgtcg tgatccccgc
    33361 actgctcggg cccgggcgga ccggggtcgg cttccggctc gcgggcttcg cgatcggcat
    33421 catggacacc cccgaggcac gccagacgat gaccgggctg ctgcgggccg ccgcctcgga
    33481 ggaggctgcc gccgtcctcg tgcgcgacct gctcatggag cgcctcctcg gcccgctggc
    33541 ccaccacctc ggaggcgacg accccacgct gcgggcgtcc ctcgccgcgt cgcaggtggc
    33601 cggcatggcc ttcacccgcc acgtcgtcgg cctgccgcgg ctcgcgaccg cctcgcggga
    33661 cgagctcgtc ggtgccctcg ggccggtgct cgagcactac ctcgtgggcg acctgtcgcc
    33721 accgcccgcc tgacgaggca caatccagcg gtggaactcg cctcggccat cgtggccatc
    33781 gtgctcgtcg tcatcgtcgt cagccgcctc gccgcacccg tcggggtgcc gacgccgatc
    33841 gcgctgctgc tcgccggggc ggtgctgtcg ttcgtgccgg ccgtgccgca gctgacgctc
    33901 tccagcgacc tcgtcctgta cggcctgctg ccgccgctgc tctacgcggc ggccctgtcg
    33961 acgtcgctgc tcgacatcag ggccaaccgg gcctccatcc tcggcctgtc cgtcgggctc
    34021 gtgctcttca cggccctggg cgtggccgtc gtcgcgtggt tgctgctgcc gatcccgttc
    34081 gccgtcgcca tcgccctcgg agcgatcgtc gcgccaccgg atgccgtggc ggccacgtcc
    34141 gtggcccgca gcatcggcct gccccggcgg gtcacgacga tcctcgaagg tgagtcgctg
    34201 ctcaatgacg cgaccgcgct cgtcaccctg cgcacggccc tggctgcggc cgggttggcc
    34261 gcccacgggg cagcgaccca tggcgaggtc cccgaggtga cggtcccgtc cgtggcgacc
    34321 gacctgctca tcgcctcggt cggtggcgtc gccatcggct ttgctgcgtt cctggcgatc
    34381 ggcgccctgc gtcgccagct gcacgaggtg ccggcagaca ccgcgctgtc cttcgtcgcg
    34441 ccgtacatcg cctacgtgcc cgccgagctc gtcggcgcct ccggggtgct gtccgtcgtc
    34501 acggccggcc tgctcctggc ccacaaggcc cccatcctgc agtcggcacc ctcacgcctc
    34561 gcggagcgga tcaactggtc gagcgtcacc ttcgtgctgg agaacgccgt cttcctcctc
    34621 atcgggctcc agatcgcatc cctgctcgaa gcggtcggcg agggcgacgg ctcactcggg
    34681 cgaacccttc tcgtcggcgg ggccgtcctc gtgacctgcc tgctgctgcg tccgctgtgg
    34741 atcgtgccgt tctccctcat cggcgcgcgg cgggcacccg accgcagcga cggcatccgg
    34801 gaaggtctcg tgggctcctg ggccgggatg cgaggtgtcg tgaccctcgc ggcggccctg
    34861 accctgcccg aggagacgcc gctgcgcgcc gagctcatcg tgatcgccct ggtggtcacg
    34921 gtcggcacgc tcctgctcca gggcctgacc ctgccctcgc tcgcccgcgc cctcgacgtc
    34981 cgcgggccgg acccgcgcga ggacgccctc gtcgaggcca ccgtgctcgg ggccacgacg
    35041 ggtgctggcc tccgcctcat cgaggccgac ccggccgccg acccgaccgc ggtcgccgcg
    35101 atccgagacc aggccagtgc ccgggtcaac cgcagctggg aacgcctcgg cacgctcggg
    35161 cccggcgacg acgagacgcc gagcgaggcc cacgcccggt tgcgcacgaa gatgatccgt
    35221 gaggagcgcg ccgagctgat gcgcatccgc ggcgagggcc acgtcgacca cgcggtgctc
    35281 agcgcggtgc tcggccagct cgacgccgag gagaccgccc tggtgtggag tgccacccgc
    35341 agcggcgccg tgcgggcctc ccccctgcgc ccgccggacc gcatcgcggg tgcctgtgag
    35401 cacctggccg ccgctgaggt ccccaaggcg ccgaccaccg ccgagggctg cccgcgctgc
    35461 cttgccgacg gggtgcgctg ggtgcagctg cgggcctgcg cgacctgcgg tgaggtcggc
    35521 tgctgcgact ccacgcccgg tcggcacgcc gaggcccact tccgcgagac cgggcacccg
    35581 gtgatgcgca gcatcgagcc cggcgaggcc tggcgctggt gctacgtcga cgagatcgtc
    35641 ggctgacccc gcgaggcacc ggtgaggtcg ccgtaggtcc tgcgcgcctt ctccagtgcc
    35701 tcgacgtagc cggcgcgctc gtaggcgctc tggtccacgc cgacggggac ggccagcagg
    35761 ccccggaact cctcgggcgt ggcgaagccc ttgcgccgca tccagtcctc cagcccgtcc
    35821 aggagcacgc cggcgtggcc gaccccgtgg cgcagcagcg cggacgccgt catgacgacg
    35881 tcggccccgg ccaggaggta cgacacgacg tccgcggccc catcgacccc ggtggtcgcg
    35941 gccagggagg cggtgacgtg cccgcgcagg atggcgatcc aggttcgggc cacgcgggcc
    36001 tcggccgggg tcgacagcgt gacccccgtc gtcaccgcga gcgcctccgg gtcgacgtcg
    36061 ggctggagga agcggttgaa cagcacgagc ccgttggccc ccgcccgatc caggcgcatc
    36121 gccatctccc cggtggagct gaagtgcggg ctcagcttga ccgccaccgg caccgagacg
    36181 gcctccctga cctgctggag gatctcgacg tggcggtcct cgacgtcgcg cgcccgggtg
    36241 tgggggtctc ccggcacgaa gtacacgttc agctcgatcg cggcggcacc ggcatcctgc
    36301 atcgagcgcg cgaaccccac ccaaccgccg gcggtgctcc cgttgaggct cgcgacgacc
    36361 ggcacctcga ccgcggccgc ggactgctcg agcagccgca ggtaccgcgt cgcaccgccg
    36421 gtcccggcgc tcgggacgtt cgggaagtag gacagcgcct ccccgaaggc gtcctcgtgc
    36481 gcctcgctga ccatgaggtc gcgcagctgc tcccggtgca cctcctcctc gaagagcgag
    36541 tacatgacga cggcacccac ccccgagtcg gccagggccc gcacccggtc cacgccctcg
    36601 gacagcgggg acgcgctggc caccagcggg ttgcgcagcg ccagcccgag gtagctcgtc
    36661 gagaggtcgc tcacctaggt cctcctcgtg tcggcggcga accggtcggc accgcgggtc
    36721 gccatctctt cgtacgtctg ccaccgctgg tcgacggcct cctgcttcag gcgcagcagc
    36781 cgttccgcct cggcggggtc ggagttgcgc agcatccggt agcgcagctc gtggtagacg
    36841 tagtcctcga gcgggaggcg gggccggggc gagtcgagct ggaacgggtt gcccccgctc
    36901 gcccgcaccg cggggtcgta gcgcacgagc ggccagtgcc cggaggcgac ggcgcggtac
    36961 tgctggtcca ggccctggcg catctcgatg ccgtgggcga tgcagtggct gtaggcgatg
    37021 acgagcgagg gcccgtcgta ggcctccgcg tcgcgcagcg cggtcaaggt ctgctggggg
    37081 tcggcaccca tcgccacccg ggccacgtac acggtgccgt acgcgatggc ctggagggcg
    37141 aggtccttct tggccactga cttgcccgcc gcggcgaact tggcgacggc gccgatcggc
    37201 gtggccttcg aggactggcc gccggtgttg gagtacacct cggtgtcgag cacgagcacg
    37261 ttgacgtctc gaccgctggc gagcacgtgg tccaggccgc cggagccgat gtcgtacgcc
    37321 cagccgtcgc cgccgacgat ccacacggtg cgccgcagga ggtggtcggc cacgctgcgc
    37381 aggtcctcca cgaccggccc ggtcatcccc tcgaggcggc gcagcagctc ggcgacccgc
    37441 tcgcgctgcg cggtgagctc gaactcgcgc acctgaccgg cctcgaggat gccgtccgcc
    37501 aggtcggcgc cgacctgatc cctcagctgg ccgagccgct cccgggccag ctccgtgtgg
    37561 aggtcggcgg ccagacgcag cccgagcccg aactcggcgt tgtcctcgaa cagggagttc
    37621 gaccaggcgg gaccgcgccc ggcggcgttc ttcgtccacg gcgtcgtcgg gaggttgccg
    37681 ccgtagatcg aggagcaccc ggtggcgttc gcgacggtga gccgctcgcc gaagagctgg
    37741 gtgagcagct tgaggtaggg cgtctcgccg cagcccgtgc acgctcccga gaactcgaag
    37801 agcggctgga ggaactgggt gccccgcacc gtgccgaagt ccacgcggga gcggtcgttc
    37861 acgggcaggg tctcgaagaa ggcgatgttc tcgcgctcgg cctcgagcac cggctcgagc
    37921 ggtgcgaggt tgatcgcctt gcgctcgggc gcccccggca tcccgggcac cacgagcggg
    37981 ctgaccgggc aggactcgac gcacagcccg cacccggtgc agtcctcgac gtagacctgg
    38041 agcgtgtagc gcgaccccgg cagcccggcg gcgttgagcg gtgccgagcg gaacccctcg
    38101 ggggcaccgt cgagggcatc gccgtcgtag aacaccgacc ggatgacgct gtgcgggcag
    38161 acgaagctgc acgtcccgca ctggatgcag gtgtcgggat cccagtcggc gacgaggtcg
    38221 gagatccgcc gtttctcgta ggcagacgtg ccgctggggt aggtgccgtc gaccggcagg
    38281 gcactcacgg gcagggcgtc accccgaccg gccatcatct cggcggtcac ggtgcgcacg
    38341 aatggcgggg cgtcgcgcgg gacaacgtcg ggcaggtccc gcaaggaggt ccgggctgcg
    38401 ggaaccggca cctcgtgcag ggccgcgagg gtggcgtcca cggccgcctc gttgcgccgc
    38461 accacctcgg caccgcgccg accgtaggtc ttgcggatcg cggccttgac ccgggcgacg
    38521 gcctcgtcgg cgggcaggac gcccgagatc gcgaagaagc aggtctggag cacggtgttg
    38581 gtccggccgg gcagcccggc gccgcgggcc acggacgtgg cgtccacggc atacaggcgc
    38641 agccgcttgt cgatgatccc ctgctggacc ggcccgggga tggcgtccca cacctcgtcc
    38701 ggcggctggg gggcgttgag cagcagggtg gccccctcct gcgcgcaggc caggacgtcg
    38761 acggtgtcga gcagcccgag gtggtggcag ccgacgaagc cggcccggct cacgaggtac
    38821 ggagcgtgga tggggtgcgg gccgaagcgc aggtgcgaga cggtgagccc gccggacttc
    38881 ttggagtcgt agacgaagta ggcctgggcg tgcacctcgg gatcggcgcc gaggatcttg
    38941 atggtgttct tgttggcccc gaccgtgccg tccgagccca gcccgtagaa gacggcccgc
    39001 agcgtctgcg ggtcctcgat gtcgagggtg gcgtcgtagg gcaggctcgt gccgccgacg
    39061 tcgtcggtga tgcccagggt gaaccggcgc cgcggcgcct cgagtgcgag ctcggcgaac
    39121 acgcccgcca ccatgcccgg cgtgagctcc ttggacgaca gcccgtaccg cccgccgatg
    39181 acccggggga tgccgtctcg ctcaccgcgc gagccggcct cggccagggt ggcgaggacg
    39241 tcgaggtaga gcggctcccc gttggagcct ggctccttgg tgcggtcgag gaccgccacc
    39301 gcgcgcacgc tgtccggcag cgcctcgacc agctccgccg acgggaaggg gcggtacagg
    39361 cgcacctgga gcaccccgac ccggtcgccg ccggcgacga ggtgggccac tgtctcgcgc
    39421 accgtctccg cggcggagcc catgacgacg acgacccgct cggcctcggg gtggccgtgg
    39481 tactcgacga cccggtacgc gcgcccggtg tggccggcga cctcgtccat gacgtcctgc
    39541 acgaccgcgg ccgtgcgggc gtagaacggg ttcacggtct cgcgcgcctg gaagtacacg
    39601 tcggggttct gcgccgtccc ccgcacgaac gggtgctcgg gcgtcagggc gcgggcccgg
    39661 tgctcgcgca cgagggcctc ggggaccacc gagcggagca cctcgtcgtc gatgagctcg
    39721 acggtgttca gctcgtggga ggtccggaag ccgtcgaaga agtggacgaa cgggacgcgc
    39781 gtgcgcagcg tggcggcctg ggcgacgagc gccaggtcgt gggcctcctg caccgacgcc
    39841 gacgccagga gggcgaaccc cgtctgccgc accgccatga cgtccgagtg gtcgccgaag
    39901 atcgacagcc cctgggcggc cagcgaccgg gccgcgacgt ggaacaccgt cgaggtcagc
    39961 tcaccggcga tcttgtacat gttggggatc atgagcagca ggccctggga cgccgtgaag
    40021 gtcgtgctga gcgcgcctcc ctggagggcg ccgtgcatcg cccccgccgc gccaccctcg
    40081 ctctgcatct ccatgaccgt gggaaccgtg ccccagacgt tggcgcgccc gtggctggcc
    40141 cactcgtcgg cgagctcggc catcgtcgag gacggcgtga tcgggtagat cgcgcagagc
    40201 tcgttgaggc ggtaggcgac gtcgaccgcc gcctcgttgc cgtcgatcgt gacgcgcatc
    40261 gggtcagccc tccgggatca tgtcgatggc gtggacgggg cactgccggt agcaggcacc
    40321 gcagcccgtg caccgggcgt cgtcgaactc gtagcgcagc ccgaccccga gcttgatgac
    40381 ggcgccctcc gggcaggcgc cgaggcagcc gtcgcactcg aagcagttgc cgcacgacag
    40441 gcagcggccg gcctcgaagt ccgcctcggt cggggacagc ccggtcagca cctcgtcgaa
    40501 ggtcagcgcc cgggtcgccg ggtcgagcac ggcctcctcg cgccggtcgt gcacgccgaa
    40561 gtaccagagg ttgagctggt ccaggctcgc gagggggtgc ttgggcgcgc ggtgcacggc
    40621 gtcaccgcgc agccacgcgt cgatcgcccg ggcggcccgc ttgccgtggc cgacgcccac
    40681 cgtgacggtc cgctggctcg ggacggcgtc gcccccggcg aagatgcccg gcgccccggt
    40741 catcagggtg tccttggcca cctgcacggt gtccccctgg aactccaggc ccgggatgcc
    40801 ccggaggaag ccggtgtcac tggcctggcc gagcgcgagg atcacggtgt cggcctccag
    40861 ggtctcgaac cgcccggtgc cgtgggggcg gccgtgctcg tcgagctcct ggacctcgac
    40921 ggtgaggtcg gtgtccccca tcgagctgat ggtgcgcagc cagttgatcc ggatgccctc
    40981 ctcctcggcg tcctgcgcct cggactcgtg ggcgggcatc tggtcgcggg tgcggcggta
    41041 gacgatcacg gtgtcgtcgg ctccgagccg gcgggcgacg cgggcggcat ccatcgcggt
    41101 gtttccgccg ccgtacaccg cgacgcgccc ggcgaggggg ggctgcgtgc ccgacgcggc
    41161 accgcggagg aactcgacgg catccatgac gcgggaggcg tcctggttgg ggatgtcgac
    41221 gcgggtggac aggtgggcac cgacggcgac gaagacggcg tcgaaggacc cgtcgcgcat
    41281 ctcggcctcg aggtcctcga cccggtggcc ctgctcgatg acgaccccga ggtcgcgcag
    41341 ccggtcgatc tcggcgtcga gcacggcgcg cggcaggcgg taggtcggga tgccgtagcg
    41401 catcatcccg cccggctcgc ccccgacgtc gcgcaccgtc accgcgtgcc cgaggcgcac
    41461 gaggtggtag gccgcggaca gcccgctcgg gccgctgccg atgacgagga cgcggtggcc
    41521 ggtggccggc ggtggcgggt cgaaggccca gccgtgctcg atggcctggt cgccgaggaa
    41581 ccgctcgacc ccgtggatgg acacggcgcc gtcgagatcg gcacggttgc acgcggcttc
    41641 gcaggggtgg tagcagaccc gcccgtggat ggcggggaag gggttgtcga gcgtcagccg
    41701 gcgccaggcg gcctcggggt cgccgccctt gaccagggcc agccacgcct ggatgttctc
    41761 gcccgcggga cagcccgcgt tgcacggcgg caggaggtcg acgtagacgg gccgctgcga
    41821 gcggacgggc cccgaccggg gccggccctg acccaccgac gcgagcgggg tgaggtcgcg
    41881 atcttctccg gccacggaca tctcctcctc gtcccgcccc gggaggaccg gacgacggca
    41941 atactacgcc gcgtagttga cgagtgccgc accgaggaga cgagcgccac gcgggggccg
    42001 ggttaggttg tcccgtatgc cgtccgcacg cctcacgctc ctgctcgcct ccgcgtcccc
    42061 cgcccgccgc acgacgctca tcgccgcggg cgtgacgccc cacgtgcggg tgtcctccgt
    42121 cgacgaggac gccgccgtcg ctgcggccga ggccatgagc gggccgctgg aggcgccaga
    42181 cgtggccctg ctcctcgccc gggccaaggc cgaggacgtc gtcagcacga tcgaggccga
    42241 cgagacgggt gacaccgatg gtgccgacga gaccgacgac ctcccggacg acctgctggt
    42301 ccttggctgc gactccgtcc tggagctcga cggtgaggtg cacggcaagc cggccgatgc
    42361 cgccgaggcg gtcgagcgct ggcggcggat gcgcgggcgc acgggcaccc tccacaccgg
    42421 gcactggctc gtcgacgccc ggcacgccga cgacggcggt accggcggga tggtgggtgg
    42481 cacggcatcc acggtggtgc acttcgctga cctcgacgac gacgaggtcg aggcctacat
    42541 cgcgacggga gagccgctgg cggtcgcggg cgccttcacg atcgacggct tgggcgggcc
    42601 gtacgtcacc gcgatcgagg gcgacccgag caccgtggtc ggtgtctccc tgccactgct
    42661 gcgcgagctg gtgcgcgagc tcggcctggc ctggcacgac ctgcgcaccc ccaccccctg
    42721 accgcgaccg tccgcgggat cagacgggag ggacgtcgcg gcgcttgtcg ctgaagtgcc
    42781 ggtcgcgccg cgccgcgtag ttcaggcgcc ggcgcagctc gtcacgcagc gcatcggcct
    42841 cgatgacctg gtcgagcacg aggtcggccg cgagccgttc gaggtcgacg tcggcctcgt
    42901 actcggcgcg ccgggcggcg acgaacgcgt cacgcgcctg caccccctcg gccgcctcga
    42961 cctcggcgat cttgttcgcg tagacggcgt tcaccgcggc ctcggggccc atgacggcga
    43021 tccgcgcggt gggcagcgcg atcgtggcgt ccgggccgaa ccccgggccg cccatcgcgt
    43081 acaacccggc gccgtaggcc ttgcgcacga cgacgcagaa ctgcggcacc gtggccgatg
    43141 ccacggccga gaccatcttc gccccgtgcc ggatgatccc gccgcgctcg acctccgagc
    43201 cgatcatgaa gccgggtacg tccgcgaggt agatgagcgg gaccgagaag gcgtcacaca
    43261 gccagatgaa ccgggtggcc ttgtcggccg agtcgctgaa gagcaccccg cccttgacgg
    43321 ccgagttgtt cgcgacgatg ccgacgctct cgccgtcgat gcgaccgaag ccgaccacga
    43381 gctcggaggc gaagagcggc ttgatctcga agaagctgtg ctcgtcgacc agtccgtcga
    43441 tgacctcgtg gatgtcgaag ggctggctct cacgctcggg cacgctcgcc cgggtcaacg
    43501 gggccgacgg ctcctcggcg tggtaggtcg gcagcgggcc gcgccagtta ccgggcaggt
    43561 aggagaagta caaccgggcg agctcgatgg cctcggcgtc gtcgtgggcg agcaggtcac
    43621 ccacaccgga gacggtgcag tgcatccgcg ctccgcccat ctcctcgagc gagaccttct
    43681 cccccacgac catctcggcc atccgcgggc tgccgaggta catggaggcg ttgccctcga
    43741 ccatgatgat gaggtcgcag aagctcggga tgtaggccc
//
LOCUS       DAOSOC010000004        38987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1082_length_38987_cov_10.081758, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000004 DAOSOC010000000
VERSION     DAOSOC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 38987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38987
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1082_length_38987_cov_10.081758"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1696)
                     /gene="pgm"
                     /locus_tag="PL014_00700"
     CDS             complement(32..1696)
                     /gene="pgm"
                     /locus_tag="PL014_00700"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HOT55637.1"
                     /translation="MNAPRAGQLAQPSDLVDVAALVTAYYTREPDPDNVDEQVAFGTS
                     GHRGSSLRTSFNEAHILATTQAICEYRRDQGFDGPLFIGRDTHGLSEPAWATALEVLV
                     ANDVTVLVDADDRYTPTPAVSHAILTANRGKITGVDGMPAGVGLADGIVVTPSHNPPA
                     DGGFKYNPPHGGPADSDATKVIAARANELIRGGLAGVKRVPFTRARAAVGTYDFLGAY
                     VDDLPNVLDMAAIRDAGIRIGADPLGGAAVDYWGAIGERHGLDLTVVNPLVDATWRFM
                     TLDWDGKIRMDCSSPSAMASLIAQRDSYDIATGNDADSDRHGIVTPDAGLMNPNHYLA
                     VAIQYLYGGARPQWRADGFIGKTLVSSSMIDRVAADLGRRLVEVPVGFKWFVPGLLDG
                     SGPFGGEESAGASFLRHDGSVWTTDKDGILLALLASEIQATTGTSPSEHYRALTARHG
                     EPAYARIDAAANREQKAKLGALSPDDVSAQTLAGEPITAKLTAAPGNGAAIGGLKVVT
                     ESAWFAARPSGTEDVYKIYAESFRGPEHLAQVQAEAKDVVSAALGF"
     gene            1789..3033
                     /locus_tag="PL014_00705"
     CDS             1789..3033
                     /locus_tag="PL014_00705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOT55638.1"
                     /translation="MSTTTKHLVVLGAGTAGTMVVNKLRKRLPATEWSITVVDKDDIH
                     DYQPGYLFIPFRMNKPGQIRKSKRPYLHDDVEVVMAEVERVDAPASTVHLVGGRTLHY
                     DQLVIASGTTPRPDQTPGMLGEQWHREVGEFYTFEGAVALRAQLDAFTGGRLVMHITE
                     LPIKCPVAPLEFVFLADDYLKGKGVREATEIVFVTPLDGAFTKPMASKRLGKLLTQRN
                     ITVETDFMIESVDQDAKVIRSYDEREVPYDQLVTVPLNMGADFVAASGLGDELNYVPV
                     DKHTCRSTQYDNIWALGDASNIPTSKAGSVAHFSVDVFVENFQDAVAGRPLSHSFDGH
                     ANCFIESGGGKALLLDFNYETEPMGGTFPLPKVGPMSLLKESRANHLGKLAFRHVYWN
                     ALLPGRPLGLPADMQTAGKVPV"
     gene            3069..3383
                     /locus_tag="PL014_00710"
     CDS             3069..3383
                     /locus_tag="PL014_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOT55639.1"
                     /translation="MSTTTIAGHEVDVNEEGFLTRPDQWSEELAPELAALIGLDLTDK
                     HWELIRFLRTDHAAMGETPTLRRVSTQTGTPVKELFALFPGKPAKKMAYVSGLPKPKG
                     CV"
     gene            3387..3926
                     /locus_tag="PL014_00715"
     CDS             3387..3926
                     /locus_tag="PL014_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOT55640.1"
                     /translation="MSDTTTTAPIVPSFDAPQSTGRKLAIICSKGNLDMAYPGLILAN
                     AAVGEGIETHLFFTFWGFDIINKATMGDLQFSFVGNTAMHPPGHSGMGVHHMLGALPG
                     ATKAATKMMKKQIADLDVPEVPEFLEILTASGAQMWACRMSADMNHLTEDDLYEGVEA
                     IISASDFMELTEGAQLLFI"
     gene            complement(3998..6259)
                     /gene="glgB"
                     /locus_tag="PL014_00720"
     CDS             complement(3998..6259)
                     /gene="glgB"
                     /locus_tag="PL014_00720"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOT55641.1"
                     /translation="MNPTPSPEVGGAITALVQGRHQQPHDLLGQHLEPGGLRIRVLKP
                     LAQSVQVRFEDGVTLDLAHEAEGVWTAVRADAERTMDYRLAVAWADGIEHVQDDPYRF
                     APTLGEVDLHLVNEGRHELLWTVLGSRLHEYDGPMGPVKGVAFAVWAPRAKAVRVIGD
                     FNDWDGRIHPMRMLGGSGVWELFVPDVGVGNIYKYEIRGADDVVRTKADPMARRTECP
                     PNTGSVVDASGHEWGDAQWMEQRAQSNPHTGPMSTYEVHLGSWRQGLSYREMAEHLVN
                     YVKDLGFTHVELLPVMEHPYGPSWGYQVTGYYAPTARFGTPDDFRYLVDTLHQAGIGV
                     ILDWVPGHFPRDAFALARFDGLALYEHPDPRRGDQPDWGTHVFDFGRLEVRNFLVANA
                     IYWLEEFHIDGLRVDAVASMLYLDYSRKDGEWIPNVHGGRENLEAIGLLQEANATAYR
                     RVPGIVTIAEESTSWPGVTKETSQGGLGFGLKWNMGWMNDTLRYLKEEPINRQYHHNL
                     LTFSLMYAFSENYLLPISHDEVVHGKGSLLTKIPGSRDEQLATVRAFLAYMWSHPGKV
                     LLFMGSEFAQPGEWADGRSLDWWLLDHAAHYRVHALVKELNALYRGHPALWAMDSEPA
                     GFRWLDADDNTGNLLSYLRFERADQSGDVVATVVNYGGEDKAWVRIGVPREGRWEVLL
                     DTSGFDEHSSPSTAGTIVEAEHVPWHDQPFSVTVRVARLSTIYLAPAVEVVESEVAAD
                     AGATDDSEAAPTA"
     gene            complement(6256..7713)
                     /locus_tag="PL014_00725"
     CDS             complement(6256..7713)
                     /locus_tag="PL014_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT55642.1"
                     /translation="MAEIITTASLRPSKLELISGWMGEQRWYAAKGQQPRLRLLAAWR
                     LDDPAGEVGVETLIVADESGPEPVVYQVPLTYRGEPLGSADHALVGTMEHTALGHRWV
                     YDAPHDPVYAAQLLELVQGRVTAQSSKQSDTVLDTVVGVPQSSWPHEVRVRASRVLSG
                     EQSNTSVILDCDLPGGQHTPLIVKVFRMLSPGDNPDVVLQGALVDAGSRRVPAVVGSV
                     HGQWPHPGADGDHPASGHLAFAQEFLPGVEDAWRVALHAAEAGTDFAGPARALGEATA
                     QVHRTLAEVLGSEETSAEQTAAILDEMRGRFEAAVEAVPALEDVRGPIESVLRAAARG
                     QWPPLQRIHGDYHLGQVLHSPERGWVLLDFEGEPLRPLAERSLPDQCARDVAGMLRSF
                     DYVGGTLELTRGESARAWVTAAQQAFLDGYAEGSGTDPRDLDVLLAAFELDKAMYEVV
                     YEARNRPGWVEIPLAAVRRLTAHFSQDPTSQGEPT"
     gene            complement(7706..9436)
                     /gene="treS"
                     /locus_tag="PL014_00730"
     CDS             complement(7706..9436)
                     /gene="treS"
                     /locus_tag="PL014_00730"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HOT55643.1"
                     /translation="MTAFPTSASATGGLRHDPQWFRTAVFYEVLTRAFHDTTGSGTGD
                     FQGVVGKLDYLQWLGVDCLWLPPFYASPLRDGGYDISDYTQVLPEFGTLPEFRELINQ
                     AHARGIRIITDFVMNHTSDQHPWFQASRSDPDGPYGDFYVWSDTDDKYTDARIIFVDT
                     ETSNWTFDPIRRQFFWHRFFSHQPDLNFENPAVHEAMFEVVRFWMDLGIDGFRLDAVP
                     YLYEEEGHNGENHPKTHAFLAQLRAMVDKEYPGRVLLAEANQPPADVVDYFGTEEAPE
                     CQMCFHFPVMPMLYYSLREERATPIIEVLADTPPIPAGAQWGTFLRNHDELTLEMVTP
                     EQRAAMYGWYAPDPRMRANVGIRRRLAPLLDNSRPEIELIHALLLSLPGSPCLYYGDE
                     IGMGDNIWLEDRDAVRTPMQWTPDRNAGFSTADPGKLYLPVITSLVYHHNHTNVEAQM
                     ATGSSLLHWVRSMLAVRREHPVFGLGDFEICTSVNDRVLSFVRVDRSERDKEDRSAMA
                     VLCVNNLSSRPQATTVTVPDELRGWETRDLFGGGGFPDIADDGTVTITLGSRDFFWLA
                     LDPPRGDVRG"
     gene            9890..12352
                     /locus_tag="PL014_00735"
     CDS             9890..12352
                     /locus_tag="PL014_00735"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOT55644.1"
                     /translation="MARGPVISPPHTVEGFVKAFLHELNFGQGVPLATSSQTDQYLAL
                     ARTVRSYLMARWLETARKRRASQPQKMVGYLSAEYLLGRQLDNALSATELREVATDAM
                     KACGIDIEDLRTQEVEPGLGNGGLGRLAACFIDSLATLGAPSIAYGIRYEFGIFRQTF
                     VDGRQVEQPDAWLSSGDPWGVPHPELEVPVNFGGHTERYVGDDGRERSRWVPGWGVIG
                     LRYNYMVPGYHNGVVNTLRLWRARATNAFDLHIFNAGDYAEAVRAQTFAENISKVLYP
                     EDSTPQGKELRLQQQYFFVACSIKDFLSHTMPKGFDVRELPERIIFQLNDTHPVIAIP
                     ELMRILVDEHDLEWDEAWGITKQCFAYTCHTLLPEALEVWPVSLMERLLPRHMEIIYR
                     INEEFLDDLREAYPGDELRVRRMSIIADHPERSVRMAHLATVGSVKVNGVAALHSQLL
                     KDKVLNDFSELWPDRFTNVTNGVTPRRFIRQSNPALTELITETIGKGWVADLDRLAEL
                     QAYSDDPEFREKFRAVKAANKERITDVLARRDGIQLPQGHMLDVMVKRLHEYKRQSLK
                     MLHIVTLYDRLVSGEVDPASITPRTVVFGAKAAPGYHMAKETIYLINSIAKTVNADPR
                     VQGRLFVAFPPNYNVTLAEKLIPAADLSEQISLAGKEASGTGNMKFALNGALTIGTDD
                     GANVEIRELVGDENFFLFGMDEPAVAELQARGYRPGDFYESDANLKRAIDLVASGHFT
                     DGNRDAVSAFVGDLLSNDRFLALADYASYLEAQDRVEAAYSDQEEWSRKAILNVARTG
                     FFSSDRSMRDYIDRIWHIKTNA"
     gene            complement(12419..14443)
                     /locus_tag="PL014_00740"
     CDS             complement(12419..14443)
                     /locus_tag="PL014_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HOT55645.1"
                     /translation="MTAPPRTSAPQHPIGRIPVLDVAPCVDHGARPAKSVVGEEFTVT
                     ATVLREGHDAVNASLVLTDPDGVEHVQVMECTNPGLNAWSAVVAADRTGWWSYRVEGW
                     SDPYGTWVHDATIKVQADVDTELMLEEGARVLERARKEVKRDKAAKAALADGIRGLRD
                     TTASAAVRLRAGTGAAVADVLSARPLRDFVSPSAELPLLVERELALSGAWYEFFPRSE
                     GATYDEKEHRWTTGTLRTAAERLPAVAAMGFDVIYLTPIHPIGQAAKKGPNNTLDARP
                     EDPGVPYAIGSKDGGHDAIHPDLGTFADFDAFVARAEGLGLEVAMDLALQCSPDHPWV
                     TEHPEWFTTRADGTIAFAENPPKKYQDIYPVNFDNDPAGIYAEVRRVVQVWIDHGVKI
                     FRVDNPHTKPVEFWQWIIADVAKDHPEVIWLAEAFTKPAMMHTLGKIGFQQSYTYYAW
                     RNTPWELREYVTELAGEAATYMRPSFWPTTHDILTPYMQFGGRAAWVVRAVLAATLVP
                     TYGIYAGYELMEHVARPGAEEQIDNEKYEYKDRRWADYEVGGPKEGQSLAGLLTQINT
                     IRANHGALHWLRNITFHSADDENILVFSKRRVYADGGEDVILVAANLDPHSTRGTHAH
                     LDLPALGLSWGDEFVAHDLLTDEQFTWGEHPFVRLGPETTPVHVIHVRRS"
     gene            14478..15011
                     /locus_tag="PL014_00745"
     CDS             14478..15011
                     /locus_tag="PL014_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55646.1"
                     /translation="MSRFVWAAWGLLVVVLDLPLFGWDVLPDIVGYLWLVVGLGGATH
                     MAFARARAAAAAGVPVALVTGTPLFVGQDGIIWGAVFIEILLTVLILHQLATAISDLA
                     PDGDQDARRWARGIRIGAPAAGVIRLIGLVGLATSLGALYALGYLGLVAVGIVTVVLM
                     HRVNRAGWLDLAPPDPA"
     gene            complement(15107..15997)
                     /locus_tag="PL014_00750"
     CDS             complement(15107..15997)
                     /locus_tag="PL014_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HOT55647.1"
                     /translation="MTATVSAPTGFEPLDPAIREQYDRDGFVVIPNALPEADRAYFED
                     AVDRIYGEEEAAGELRPDRSIHVMGWMHKDPRFVELLNWPQTFPYIWGTMGWNIYTHH
                     NHIDVNPPKDEPPFWNWHQDGYRQNSDIDMDVRPMFMTKICYALTDLSEPGYGNTKVI
                     PGSHKNNTLAGRPEKEGDPIIEPEGAVEVLLNPGDAFIFDRRLWHSRSMNKSERIRKL
                     MFIGYTYRWIRPLDEAVADQSSEWFKGLSPLHQQLLGYGPDHASFWGIKQSGWIDDEI
                     PLRAELKSRGLLDRNIPFLR"
     gene            complement(16148..18310)
                     /gene="glgX"
                     /locus_tag="PL014_00755"
     CDS             complement(16148..18310)
                     /gene="glgX"
                     /locus_tag="PL014_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOT55648.1"
                     /translation="MSPWSHPTTIPPGPMTRDLAPRPGVTLVEGGADVAVYAGHADAV
                     ELCLFDAGDKAGTSERRIPLLERAHGWWFAFVPGITAGQRYNFRAHGAWSPDQGLRHN
                     PAKLLLDPYAKAIEGKVRWGAEVYGHVVDVRWHGDGELISDLDSRGHMPRGVVVDDRF
                     DWEGDVAPNRSRSESVIYEAHVRNQTALHPGVPPELRGTYAGLAHPASVAHLTSLGVT
                     AVELLPVHAFTHEPHLVRRGLTNHWGYNTLGFFAPHGAYAAAKDPQGAVDELKGMVKL
                     LHGEGIEVILDVVYNHTAEQGRTGAMLSWRGLDQRAYYRLDERGRDIDVTGCGNTLDL
                     RHPVVCRMVLDSLRYWVQEYHVDGFRFDLAVALGRGRGDDFDPDHPFLVALRTDPVLS
                     AVKLIAEPWDVGMHGWRTGQFPPPFMEWNDRYRDAVRRFWVGDVRSQRHHRRGHGLQE
                     LATRLAGSRDLFGHRDRGPTASINFVAAHDGFTTADLVAYDTKHNEANGEDNRDGSDN
                     NGSWNHGTEGPSDDPQVIAARTLAIRNLMGTLLLSTGVPMINAGDELGRSQGGNNNPY
                     CQDNGTSWINWDLEPWQQDLLDTTSHLIRVRQALPVLRQRVWALGRQVHDDGTRDMEW
                     YAADGTPMGDRWTQGSRLVQLYVAGAWMGWDSALLVVNGGVEDVEVTLPEAPGVTTYR
                     LLWDSTWSRPRDGGDPVVPGPVTVGATSLRVYAASDVS"
     gene            18345..18818
                     /locus_tag="PL014_00760"
     CDS             18345..18818
                     /locus_tag="PL014_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55649.1"
                     /translation="MTDPTSLPNFPPPADEHPLRGRVLDILVDLGLAPNLDTDGDVAF
                     TVNEQQLFIRCTEGEVEIMRVFGQWQIQEEMLGDRLKLHETCNELNLNMNCLKSGVAG
                     STLVVTSEHLATPGADVSSLIQVSIQVILSGVHIWHQRLLGIDPNAPESDAGSPS"
     gene            18815..19594
                     /locus_tag="PL014_00765"
     CDS             18815..19594
                     /locus_tag="PL014_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT55650.1"
                     /translation="MTDLVRLEVLDGIGTIRLDRPPMNALNAEIQHGLIAACREAAER
                     KDVAAVIVWGGEKVFAAGADIKEMETMSYTDMVDHSALLQDFTRSLAQLPRPTVAAIT
                     GYALGGGCEVALACDFRIAADDAVLGQPEVSLGIIPGAGGTQRLARLVGPSRAKDLVF
                     SGRFVKAPEALAIGLVDSVVPASEVYESARSMMARYVGGPAYALRAAKEAIDRGLEVD
                     LDSGLEIERMLFSALFATKDRAIGMRHFVDKGRGPAPFEGA"
     gene            complement(19619..20890)
                     /locus_tag="PL014_00770"
     CDS             complement(19619..20890)
                     /locus_tag="PL014_00770"
                     /inference="COORDINATES: protein motif:HMM:NF021524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HOT55651.1"
                     /translation="MVRTTDTEPGGARRTVGPVGDPETIGWWERLWAPFWVLPSTICA
                     LAVVAGVVLPRVDEALTEDLPYLFGGGAESARSLLSTIASAMISVTGLVFSITMVVMQ
                     LASSQFTPRLLGSFLSSRITQVTLGVFTSSFVFALIVLRDVRGGDDPFVPQLSVTVAF
                     LLVLASVALFFAFIHHITVSIQVSKVIDRVADQTVRALQQGDDEWGDTSTPSPSWSQA
                     PGPSVTVSAGSGHGVVQRVRYIDLVETARRAGVVVELTTRVGEVRHRGQSLAVVHGAF
                     ADDDVIDKVRSSFGLGKERSMPQDPEFGVRQLVDIAERALSPGINDPTTAVQVLDELH
                     RILRVAGSRADRSPHLVDADGTVRVLDLPTTFARLLDLSLDEIAHYGKDSLQVPRRID
                     TLLDDLWGSVRDEHGAAVTAKRTALQDALGA"
     gene            21135..21926
                     /locus_tag="PL014_00775"
     CDS             21135..21926
                     /locus_tag="PL014_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT55652.1"
                     /translation="MNIVVCVKHVPDAQSERTFRESDSTIDREGVDGLLSELDEYAVE
                     EALKLVEAAGEGEVTVLTVGPAGAADAIKKALQMGAHAGIHVSDEAIHGSDAAATSLV
                     LAEAVKKATGGNPELVLTGMASTDGVMGVVPAMLAERLGLPQVTFASELTIADGTVTI
                     RRDGDTASETIVASLPALVSVTDQINEPRYPSFKGIMAAKKKPVEQWGLADLGIDPSL
                     VGLDAAWTTVVSFAKRPPRAQGQIVTDGGDGGTQLAEFLAGQKFL"
     gene            21961..22917
                     /locus_tag="PL014_00780"
     CDS             21961..22917
                     /locus_tag="PL014_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT55653.1"
                     /translation="MAEVLVLVDHADGRLRKATAELLTIARRLGEPSAVHIGPGADRA
                     QVTLAKYGAHKVYVVDDADAAGFLVAPQAEILAALVEKVAPAAVIIPSNAAGKEIAAR
                     LAIKTSSGLITDAVDVRAGEGGIETTQSVLAGSYTVTAKVTTGTPIIAVKPNSVPLEA
                     SPAPGVVEAFAVTVSDAAKAARVTDSQPKKATGRPELTEAAIVVSGGRGTGGDFSPVE
                     EFADSLGAAVGASRAAVDAGWYPHAHQVGQTGKQVSPQLYVAAGISGAIQHRAGMQTS
                     KTIIAINKDEEAPIFELVDFGVVGDLFSVLPQATAEVKKRKG"
     assembly_gap    23023..23122
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23384..24562
                     /locus_tag="PL014_00785"
     CDS             23384..24562
                     /locus_tag="PL014_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOT55654.1"
                     /translation="MTHYLDHAATTPMLPAAVDALLEVSRQVGNPSSLHTSGRAARAV
                     VEEARERIGAALGARPSEVVFTSGGTEADNLAVKGTWLARRAKDPSVNGVVASSIEHH
                     AVLDPVEYLEDSRGVPVTWLEPDRCGHLAPDTLRAALAAGGVAVASVMWANNEVGTIQ
                     PVAELAAVAREHGVPFHTDAVQAIAHLPVRFDDSGADLMTVSAHKIGGPVGVGALLAR
                     RDAPLVPIAHGGGQERQVRSGTLDAAGIHSFAVALEETVAHRDVEAKRVLALRDRLIE
                     GALGLGLGITVSGCWKAGDATKRLPGNAHLLVPGCEGDSLLYLLDAAGVECSTGSACQ
                     AGVPQPSHVLLAMGHTEAQARGALRLSLGHTSTEADVDAFLSALPGVVERARRASGLI
                     "
     gene            24562..25650
                     /gene="mnmA"
                     /locus_tag="PL014_00790"
     CDS             24562..25650
                     /gene="mnmA"
                     /locus_tag="PL014_00790"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOT55655.1"
                     /translation="MRVVAAMSGGVDSAVAASRMIEAGHEVVGVHLALSRSAATLRES
                     ARGCCTIEDAGDARRVADRLGIPFYVWDLASRFERDVVEDFAAEYAAGRTPNPCLRCN
                     ERIKFAGLLDKAVALGFDAVATGHYAQIVQGPTGRELHRAVDAAKDQSYVLGVLDADQ
                     LARSFFPLGDSTKTQVRAEAAERGFAVAKKPDSHDICFIPDGDTRGWLNRRLGERPGE
                     LVDAVSGAVVGSHTGAHGFTVGQRRGLGIDRSALDGDPRYVVEIDAPSNRVVIGTADL
                     LGVDLIVGDHVRWCGPAPGERVALGAQVRAHGEEVPATAEVQAGPSPTVRVGLERRVR
                     GVAPGQSVVLYEGTRVVGSATISETGRA"
     gene            25845..26819
                     /locus_tag="PL014_00795"
     CDS             25845..26819
                     /locus_tag="PL014_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOT55656.1"
                     /translation="MPAVTVENILTLPRVSQPVPDAVTRPVRSVTTAPSGFEGEGFPV
                     RRAFAGVDLAALDPFIHMDQMGEVEYAPGEPKGTAWHPHRGFETVTYIIDGTFEHRDS
                     HGGGGTITNGDTQWMTAGSGLLHIEVPPESLVLSGGLFHGLQLWVNLPGSMKMSNPRY
                     QDIRGGEVALLSTHDGGGLVRVIAGEVDGHQGPGITHTPISIVHATIEAGGQLRLPWN
                     PQFNALVYVLNGLGTVGPDRRPIRQGQLVVLGAGDVIEVGANEQQESRSPSLDLYIMG
                     GRPIREPVAAYGPFVMNTRAELVQAFEDFQAGKLGTIPSEPHPGHSFS"
     gene            complement(26827..27600)
                     /locus_tag="PL014_00800"
     CDS             complement(26827..27600)
                     /locus_tag="PL014_00800"
                     /inference="COORDINATES: protein motif:HMM:NF039623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HOT55657.1"
                     /translation="MGDVVSAERRLESMTQVWTASGDRRAVFSECYLVMTRRVRERVR
                     NGAFHDGVWVTRLLDRFADYYFDAVEAYGRSGDFTLTTVTTVTTTPDGVEATVTPVPE
                     RTCPEIWRGALDACSDPGCHPLQALMLGINAHINHDLAPALVDVLDDWGTLEEEQRRH
                     RHEDHELVNTIIDETTDEVQRDVVAQWSPLAEMLDALMGPLDEWAFGTLAQSWRSQVW
                     LDAMDLVALPPEERPLATQRIGERASGVATVIMLGRRPG"
     gene            complement(27631..29772)
                     /locus_tag="PL014_00805"
     CDS             complement(27631..29772)
                     /locus_tag="PL014_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOT55658.1"
                     /translation="MPATTPRRAAHGADGRAGPELLVVEVSLGSSARDHDLRVRFLDR
                     RYRLLRLGTDGDVHTAQALVRRWSELADQVAVNGGAEAAATGLLDHDPAEATARLVAS
                     AVGGTATDGHRLREVLQEWAVRRVQYEMPGYFSNARTVVLGSDHARTVRVLREYTDNL
                     HLEAPPVGAGPGRRLGGVPVLGRVARLAWHPVDRVRSAVGDDSLTPGELVNRSWLRQR
                     PRDCDVVVATYHELVTFGFPDLHGKTVITSAVTDAQLADLASRDVDLVLDSTPQPFES
                     TVDVATLEAMMRAAVRAPGGAPERLSDDDLLEMIVAAGLEPRVLQPNGPRRKSRFAFV
                     IHPLSQKFFRNVEPLGTVAKVSPPVVMDAVEKGMAYLPPFVYGHVTGVRSPTGAEAEG
                     WLITVGGTPKEILAHDPEFTYDKLLEAAELARRLGAQVMGLGAFTKVVGDAGVTVARK
                     ASLPITTGNSYSASGALWAAHDAVDRLGLLERDDDGVIRGRAMVVGATGAIGSVCARL
                     LALASDELWLVSPETAKLLALKKDIEREHPRAVVHAAATPGDHLRHMDVIVTATSGAG
                     KRVLDIMDVKPGCVITDVARPLDLSAEDVARRPDVLVVESGEIELPGEVRMRDIGLPT
                     GVVYACLAETVALALEGRYETFTVGRAIEWEKVKEIYQLGLRHGMRLAAISGVKGVYT
                     DEDFARVRELALAARGELAAAGPRGRGRKAT"
     gene            complement(29795..30871)
                     /locus_tag="PL014_00810"
     CDS             complement(29795..30871)
                     /locus_tag="PL014_00810"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT55659.1"
                     /translation="MTGFPGFLGSALLPRLLARRPGTDAVCLVQRQHGALARSRVKEL
                     EVTDPGTAGRVRLVVGDVTASGLGLSAADRAGLDDVDEVWHLAAVYDLAVAPEVARRV
                     NVEGTRHVLEFCRGLRDLRRLQYVSTCYVSGRYEGCFAEDDLEEGQAFRNHYERTKYD
                     AEVLVRAAMASGLPATVYRPGIVVGDSATGETQKYDGPYFLATFLLRQLSVAVVPALG
                     DADRVRVCLVPRDFVVGAMDELSVLEESRGRTYALVDPDPPTVREVVTAFARHLGKRV
                     VWVPLPLAPVHALVDHVPGAQALLGLPAEALDYFASPTTYDTTTTTTDLAASGLTCPA
                     FESYADTLLDFMGAHPEFDAHAMT"
     gene            complement(31035..32468)
                     /locus_tag="PL014_00815"
     CDS             complement(31035..32468)
                     /locus_tag="PL014_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT55660.1"
                     /translation="MPRTPRPVELALTVPAGAAPLRHRIADAVVGELRSGRLRPGDPL
                     PSTRVLAADLGLSRGPVVAAYDELAAAGFVLSRAGSGAVVAPGADRAAAAGALSHVPT
                     AGGEAVGIRSVATAPPRWDLRPGRPDTTLLDAAAWRRAWRAAGTAVPGNDAGAGPSHE
                     GLRRVLADHLRRSRGVAVAPDDLLVVPGVGASLRALPVALGLVGSTVALEDPGYVEAW
                     TAFEAEGARVAAVSVDGDGLDPAHLPRSARAVYVTPSHQYPLGARMPVTRRALLLDWA
                     RRTDSLVLEDDYDGEFRYDVSPLPALRSMPGAADHVVYIGTASKLIAPSLRVAWIAAP
                     RRLRERLRVELESRGLVVNEATGLALAEFIASGSLATHHARVTRTYAARRAALVAAAS
                     DHLPFVVLDGVEAGLHVVLRLPDDVDDLDVVTRLEQVGVAVSPLSAYAVATPVRGLVL
                     CYAGLPETQADAAVRALAGAIGPDALT"
     gene            32586..33509
                     /locus_tag="PL014_00820"
     CDS             32586..33509
                     /locus_tag="PL014_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HOT55661.1"
                     /translation="MSWLQMRPGCECCDIDLPAGEVGAVVCSFECTFCSGCAEHLERA
                     CPNCGGALMPRPTRVGDALLRHPASTTRVVSAGECPGRGYAGRGSVAAGASRQRLRRL
                     TDRARSERADLDALLDEVLTGTLSTVVDGRPWVVPMLFARDGDRVLLHGSTGAGALRH
                     VAAGAPAVFSVMVVDGLVVAHTTFDSSANYRSAVIHGVLTPLTGEDRETSLDRISERL
                     IPGRTAEVRPMLPKEVAATLAMELPITDGQWVLKARSGGASVPDEETDAWSGVVPLRV
                     VAGDPEPAPWSVEAATPVPASVSAFVERVGR"
     gene            33506..34504
                     /locus_tag="PL014_00825"
     CDS             33506..34504
                     /locus_tag="PL014_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOT55662.1"
                     /translation="MTAPVRATGIGSWPGASVREAVRTVRDLLLTGDGDGLPHLPETP
                     ARGPGADIIGRAAGLLVDLPVDLQPSGWRLVDRPGRDSGRTAALHREDLDELAEAYDG
                     YVGPLKVQVAGPWTLVASLRLNRGERVISDAGAVGDVTASLSDGIQNYVADVRRLVPG
                     ADVVLQVDEPSLPAVLEGSLPTSSGYGRVRAVEPHDVMRRLEQVLAAHDGETVVHCCH
                     PSAPLPLLRATGAGALAIDVTSATPARWESIAATLEAGTGLYAGCLPTDGSGTDQGAR
                     ALVLEGFERAGLAASSLTGLVVSPACGLAGLTQQGARAVVRTALDTARRLSEEVPA"
     gene            34501..35490
                     /locus_tag="PL014_00830"
     CDS             34501..35490
                     /locus_tag="PL014_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT55663.1"
                     /translation="MTTQPRVPWQVKYLALVLIWGSSFLLMKVGLEALTAVQISALRI
                     LSGAVVLLGLLFARGGRLPTGARVWGHLLVCGFFLGALPFTLFALSETRISSALAGIG
                     NAATPIATVLATMAILPGERATGRRLVAVGVGFLGVITIMQPWTATDRPDLVGFSMAL
                     IGGASYGIGWTYNRKFLSGVDLGGLSQPAATLTSALVLMVPFVVTWALLQPEGLAAIW
                     AYDAAGVGDLPWLPLLCVLVLGVVGTGFAYMLQFDVVRGAGPVIGSTITYLIPVVSVL
                     LGVLVLGEHLGPWQIVGFVIVLGAAFVINQKPAARPATDATASTVGEVTTTAR"
     gene            complement(35554..37050)
                     /locus_tag="PL014_00835"
     CDS             complement(35554..37050)
                     /locus_tag="PL014_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT55664.1"
                     /translation="MTNLATNLTSTAATNAEQDALRINGQGVTYAQLHAMAAAVAGAL
                     RANGIQPGDRVAIILPNVPAFPVVYWGILLAGGIVVPMNPLLKAGEIDYFFTDSGAKI
                     AFVWPDFVPEATKGAQNSGTQIIECGPMGPVAGSFEGGDPITDPHERADDDTAIILYT
                     SGTTGRPKGAELTHSNIHLNALRSAKVIQQMTADDIVMGCLPLFHVFGLVVGLNAATI
                     AGASLALIPRFDPAAAIEVIEKERVTIMQGVPTMYAAILNHPASDGMDASSLRTCASG
                     GSAMPLEVMRSFEEKFGCVILEGYGLSETSPVASFNMPTMERKPGTIGVAIPGCEMKC
                     VDLDGNEVPVGEVGEIAIRGDNVMKGYWNKPEATAEAIPDGWFRTGDLATMDSEGYFT
                     IVDRKKDMILRGGMNVYPREVEECIYEHPDVLEVAVVGIPDELYGEQVGAAVALREGS
                     TATPQDIIDFAKERIAAYKYPRTVWVVPELPKGPTGKILRREVHAPAE"
     gene            37116..38015
                     /locus_tag="PL014_00840"
     CDS             37116..38015
                     /locus_tag="PL014_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT55665.1"
                     /translation="MASWQMRAVALYVRATRKKRYATREAGLAFLHSAKGPSDPPAKA
                     LGDSRVSTHRVRGRDVHVVRGPGHTASAAGAVVYLHGGAYTNEVVDQQWAFVAALAAR
                     TGVEVHVPIYGLAPHHTASEAVDLVAEVVHGLVAQGRSVVLLGDSAGGGLALVAAQQA
                     APAVRQRVAGLLLIAPWIDLTMANPAVDAVEPTDPWLTRAGLHPIARSWAGDLALDDP
                     VVSPLFGDLTGLPPVEVWVGTRDITAPDCRELERRMPDGAAFALHVEDGAIHDYPLLP
                     TPEGRAASREIGERVTALLTASG"
     gene            complement(38031..>38987)
                     /locus_tag="PL014_00845"
     CDS             complement(38031..>38987)
                     /locus_tag="PL014_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOT55666.1"
                     /translation="SDFDPKGRSDTEIMRFCQSFMTELYRHIGEYTDVPAGDIGVGGR
                     EIGYLFGQYKRITNRYESGVLTGKGISVGGSQVRTEATGYGTVYFAREMLATAGQSFD
                     GKRVVVSGSGNVAIYAIEKVAQLGGTVVACSDSGGYVVDETGIDLDLLKEVKEVRRGR
                     LSDYAEARGGSARHVVGGSIWDVPCQVALPCATQNELPESGARALIANGVELVAEGAN
                     MPTTPEAVALLQEAGVAYAPGKASNAGGVATSALEMQQNASRDSWTFEATEERLEAIM
                     TSIHAQCVETAEQYGDPGNYVMGANLAAYTKVADAMVLLGVI"
BASE COUNT         5577 a        13626 c        13923 g         5761 t
ORIGIN      
        1 gggtgtgggg acggtggggc gtgaggtggg gtcagaagcc gagggctgct gagacgacgt
       61 ccttggcctc ggcctgcacc tgggccaggt gctcggggcc acggaaggac tcggcataga
      121 tcttgtagac gtcctcggtg ccactgggcc gggccgcgaa ccacgccgac tcggtgacga
      181 ccttgaggcc gccgatcgcc gcaccgttgc cgggagccgc ggtcagcttg gccgtgatcg
      241 gctcaccggc cagagtctgc gcgctgacgt cgtcgggtga cagcgcaccg agcttggcct
      301 tctgctcgcg gttggcagcg gcgtcgatgc gggcgtacgc cggctcgccg tgccgggcgg
      361 tcagcgcccg gtagtgctcg ctcgggctcg tgcccgtggt ggcctggatc tcggaggcga
      421 gcagggccag caggatgccg tccttgtcgg tggtccacac cgacccgtcg tggcgcagga
      481 acgacgcacc ggccgactcc tcaccaccga acggccccga gccgtcgagc agcccgggca
      541 cgaaccactt gaacccgacc ggcacctcga cgagccgccg cccgaggtcg gccgccaccc
      601 ggtcgatcat cgagctcgac acgagcgtct tgccgatgaa gccatccgcc cgccactgcg
      661 gccgcgcccc gccgtagagg tactggatcg cgacggcgag gtagtggttg gggttcatca
      721 ggccggcatc cggggtgacg atgccgtggc ggtcggagtc ggcgtcgttg cccgtggcga
      781 tgtcatagga gtcgcgctgc gcgatgaggc tggccatcgc cgagggggag gagcagtcca
      841 tccggatctt gccgtcccag tccagcgtca tgaaccgcca cgtcgcgtcg acgagcgggt
      901 tgacgacggt gaggtcgagc ccgtggcgct caccgatcgc accccagtag tcgaccgcgg
      961 ccccgccgag cgggtcggcg ccgatgcgga tgccggcgtc gcggatcgcg gccatgtcca
     1021 ggacgttggg caggtcgtcg acataggcgc ccaggaagtc gtaggtgccg accgcagcgc
     1081 gggcacgggt gaacggcacc cgcttgaccc cggccagccc gccgcggatc agctcgttcg
     1141 cgcgggccgc gatcaccttc gtcgcgtcgg agtcggcggg cccaccgtgg ggcgggttgt
     1201 acttgaagcc gccgtcggcc ggggggttgt gcgagggcgt gacgacgatg ccgtccgcga
     1261 ggcccacgcc ggccggcatc ccgtcgacgc cggtgatctt gccgcggttg gcggtgagga
     1321 tggcgtgcga gaccgcaggg gtcggggtgt agcggtcgtc ggcgtcgacg aggacggtga
     1381 cgtcgttggc gacgagcacc tcgagagcgg tggcccacgc cggttcggac agaccgtggg
     1441 tgtcgcgccc gatgaacagc gggccgtcga aaccctggtc gcggcggtac tcgcagatcg
     1501 cctgcgtcgt cgcgaggatg tgcgcctcgt tgaacgaggt gcgcaggctc gatccccggt
     1561 gtccagaagt gccgaatgcg acctgctcgt cgacgttgtc ggggtccggc tcgcgcgtgt
     1621 agtacgccgt gacgagcgcg gcgacgtcga cgaggtcaga gggctgggcg agctgtccgg
     1681 cacggggcgc gttcatggtg gtgatcctgt cagaggtgtc tacgagatca caccccgtgg
     1741 agcagtcttc cgccgttccg gtcataccca caggggtatc gtcgaagtat gtccaccacc
     1801 acgaagcacc tcgtggtcct gggagcgggc acggccggga cgatggtcgt caacaagctc
     1861 cgcaagcggc tcccggccac cgaatggtcc atcaccgtcg tcgacaagga cgacatccac
     1921 gactaccagc cggggtacct gttcatcccg ttccggatga acaagccggg ccagatccgc
     1981 aagagcaagc gcccctacct gcacgacgac gtcgaggtcg tcatggccga ggtcgaacgg
     2041 gtcgacgccc cggcctccac cgtgcacctc gtcggcggac gcaccctgca ctacgaccag
     2101 ctcgtcatcg ccagcggcac gaccccccgc cccgaccaga cccccgggat gctcggcgag
     2161 cagtggcacc gcgaggtcgg tgagttctac accttcgagg gtgcggtcgc gctgcgggca
     2221 cagctcgacg cgttcaccgg tggccggctg gtcatgcaca tcaccgagct gcccatcaag
     2281 tgcccggtgg cgccgctgga gttcgtcttc ctcgcggatg actacctcaa gggcaagggc
     2341 gtgcgcgagg ccacggagat cgtcttcgtc acccctctcg acggtgcgtt caccaagccg
     2401 atggccagca agcggctcgg caagctgctc acgcagagga acatcaccgt cgagaccgac
     2461 ttcatgatcg agagcgtcga ccaggacgcc aaggtcatcc ggtcctatga cgaacgcgag
     2521 gtgccctacg accagctcgt caccgtcccg ctgaacatgg gcgcggactt cgtcgcggca
     2581 tccggcctcg gcgacgagct caactacgta ccggtcgaca agcacacctg ccgctcgacc
     2641 cagtacgaca acatctgggc cctgggcgac gcgagcaaca tccccacctc caaggcaggg
     2701 tcggtggcgc acttctccgt cgacgtcttc gtcgagaact tccaggatgc cgtcgccgga
     2761 cgcccgctct cgcactcctt cgacgggcac gccaactgct tcatcgaatc cggtgggggc
     2821 aaggcgctgc tgctcgactt caactacgag accgagccca tgggtggcac cttcccgctg
     2881 cccaaggtcg ggccgatgag cctgctcaag gagagccgcg ccaaccacct cggcaagctc
     2941 gccttccggc acgtctactg gaacgccctg ttgcccggcc gccccctcgg cctgccagcc
     3001 gacatgcaga ccgccggcaa ggtcccggtc tgaacccctc gagatcgaca cgaaaggaca
     3061 tcaccgtcat gagcaccacc acgatcgccg gccacgaggt cgacgtcaac gaggaggggt
     3121 tcctcaccag acccgaccag tggagcgagg aactggcccc cgagctggcc gccctcatcg
     3181 gcctcgacct caccgacaag cactgggagc tcatccgctt cctgcgcacc gaccacgccg
     3241 ccatgggtga gacgcccacg ctgcgtcgcg tgtccaccca gaccggcacc ccggtcaagg
     3301 agctgttcgc cctgttcccc ggcaagcccg cgaagaagat ggcctacgtc tccggcctgc
     3361 ccaagccgaa gggatgtgtc tgagccatga gtgacaccac gacgaccgcc cccatcgtcc
     3421 cgtcgttcga cgccccgcag agcaccggtc gcaagctcgc gatcatctgc tccaagggca
     3481 acctcgacat ggcctacccc ggcctgatcc tggccaacgc cgcagtcggc gagggcatcg
     3541 agacccacct gttcttcacc ttctggggct tcgacatcat caacaaggcc acgatgggtg
     3601 acctgcagtt ctccttcgtc ggcaacaccg cgatgcaccc tccgggccac agcggcatgg
     3661 gcgtgcacca catgctcggt gcgctgcccg gggcgaccaa ggccgcgacg aagatgatga
     3721 agaagcagat cgccgacctc gacgtgcccg aggtgccgga gttcctcgag atcctcaccg
     3781 cctccggtgc ccagatgtgg gcctgccgga tgtcggctga catgaaccac ctgaccgaag
     3841 acgacctcta cgaaggggtc gaggcgatca tctcggcgag cgacttcatg gagctcaccg
     3901 agggtgccca gctgctgttc atctgaccca ctgccctggg gcagtagaca gcgcgggccg
     3961 gggcccggcg ggagagatct cccgccgggc ccgaacgtca cgccgtgggt gcggcctcgg
     4021 agtcgtcagt ggccccggcg tcagcggcga cctcggactc gacgacctcg acggccggtg
     4081 cgaggtagat cgtcgagagc cgcgcgaccc gcaccgtgac ggagaacggc tggtcgtgcc
     4141 acggcacgtg ctcggcctcg acgatcgtgc cagcggtgct cgggctgctg tgctcgtcga
     4201 acccgctcgt gtcgagcagg acctcccagc ggccctcgcg gggcacaccg atgcgcaccc
     4261 acgccttgtc ctcgccgccg tagttgacca ccgtcgcgac gacgtcgccg ctctggtcgg
     4321 cccgctcgaa acgcaggtag gacagcaggt tgccggtgtt gtcgtcggcg tcgagccaac
     4381 ggaaccccgc cggctcggag tccatcgccc acagcgccgg gtggccgcgg tagagggcgt
     4441 tgagctcctt gacgagcgca tgcacccggt agtgcgccgc gtggtcgagc agccaccagt
     4501 cgagcgatcg gccgtcggcc cactcgccgg gctgcgcgaa ctcactgccc atgaagagca
     4561 gcaccttgcc ggggtgggac cacatgtacg cgaggaacgc gcgcaccgtc gccagctgct
     4621 cgtcgcgcga gcccgggatc ttcgtgagca gcgagccctt gccgtgcacg acctcgtcgt
     4681 ggctgatcgg caggaggtag ttctcgctga aggcgtacat cagcgagaag gtcagcaggt
     4741 tgtggtggta ctgccggttg atcggctctt ccttgaggta gcgaagggtg tcgttcatcc
     4801 accccatgtt ccacttcagg ccgaagccga gcccgccctg cgaggtctcc ttcgtgaccc
     4861 ccggccagga ggtcgactcc tcggcgatgg tcacgatgcc gggcacccgc cggtaggccg
     4921 tcgcgttggc ctcctggagc aggccgatcg cctcgaggtt ctcgcgcccg ccgtgcacgt
     4981 tggggatcca ctcgccgtcc ttgcgcgagt agtcgaggta gagcatcgag gccacggcgt
     5041 cgacgcgcag cccgtcgatg tggaactcct ccagccagta gatggcgttg gcgacgagga
     5101 agttgcgcac ctcgaggcgc ccgaagtcga agacgtgggt gccccagtcg ggctggtcgc
     5161 cgcggcgcgg gtccgggtgc tcgtacaggg cgaggccgtc gaagcgggcc agggcgaacg
     5221 cgtcacgcgg gaagtgcccg ggcacccagt cgaggatgac gccgatgccg gcctggtgca
     5281 gcgtgtcgac caggtagcgg aagtcgtcgg gggtgccgaa ccgggccgtc ggggcgtagt
     5341 acccggtgac ctggtagccc caggacgggc cgtacgggtg ctccatgacc gggagcagct
     5401 cgacgtgcgt gaagccgagg tccttgacgt agttgacgag gtgttcggcc atctcgcggt
     5461 aggacagccc ctgtcgccac gagcccaggt gcacctcgta ggtgctcatc gggccggtgt
     5521 gcgggttgct ctgcgcgcgc tgctccatcc actgcgcgtc accccactcg tgccctgagg
     5581 cgtcgacgac cgacccggtg ttcggcgggc actcggtccg gcgggccatg gggtcggcct
     5641 tggtgcgcac gacgtcgtcg gcaccgcgga tctcgtactt gtagatgttg ccgacgccga
     5701 cgtcggggac gaagagctcc cagaccccgg acccgccgag catgcgcatc gggtggatgc
     5761 gcccgtccca gtcgttgaag tcaccgatga cgcgaacggc cttggcccgc ggggcccaga
     5821 cggcgaacgc cacgcccttg accggcccca tcgggccgtc gtactcgtgc agccgcgatc
     5881 cgagcacggt ccacagcagc tcgtggcgac cctcgttgac caggtgcagg tcgacctcgc
     5941 ccaaggtcgg cgcgaagcgg tacgggtcgt cctggacgtg ttcgatgccg tcggcccagg
     6001 ccaccgcgag gcggtagtcc atggtgcgct ccgcatcagc cctcaccgcc gtccacacgc
     6061 cctcggcctc gtgggcgagg tcgagggtca cgccgtcctc gaagcgcacc tgcaccgact
     6121 gggcgagggg tttgaggacc ctgatccgca ggccgccggg ctcgaggtgc tgccccaaca
     6181 ggtcgtgcgg ctgctggtgg cggccctgga cgagcgcggt gatggcgccg ccgacctcgg
     6241 gactgggtgt ggggttcacg tcggctctcc ttgactggtg ggatcctgcg agaagtgtgc
     6301 cgtgaggcgg cggacggcgg cgagggggat ctcaacccag cccgggcggt tgcgggcctc
     6361 gtagacgact tcatacatcg ccttgtccag ctcgaacgcc gcgagcagca cgtcgaggtc
     6421 gcgcgggtcg gtgcccgacc cctccgcgta gccgtcgagg aacgcctgct gcgccgctgt
     6481 cacccacgcg cgggcggact ccccccgggt cagctccagg gtgccgccga cgtagtcgaa
     6541 gctgcgcagc atccccgcga cgtcgcgagc gcactggtcc ggcagcgacc gttccgccag
     6601 cggccgcagc ggctcaccct cgaagtcgag cagcacccag ccgcgctcgg gcgagtgcag
     6661 cacctggccg aggtggtagt cgccgtggat gcgttgcagc gggggccact gacctcgggc
     6721 agcagcgcgc agcaccgact cgatggggcc gcgcacgtcc tcgagcgccg ggaccgcctc
     6781 gacggcggcc tcgaaccggc cgcgcatctc gtcgaggatc gcggcggtct gctcggcgct
     6841 cgtctcctcg ctgcccagca cctcggcgag ggtgcggtgg acctgtgccg tggcctcgcc
     6901 gagcgcgcgg gccggccctg cgaagtcggt gccggcctcc gcggcgtgca gcgccacccg
     6961 ccaggcgtcc tcgacgccgg gcaggaactc ctgggcgaag gccaggtggc cggatgccgg
     7021 gtggtcgccg tcggcgcccg ggtgcggcca ctgcccgtgc acggacccga ccacggcggg
     7081 aacgcgacgc gacccggcat ccacgagcgc gccctggagg acgacgtcag ggttgtcgcc
     7141 gggggagagc atccggaaga ccttgacgat gagcggcgtg tgctggccgc ccggcaggtc
     7201 gcagtcgagg atgacggagg tgttggactg ctcgccactg agcacccgcg acgcccgcac
     7261 ccgcacctcg tgcggccagc tggactgcgg gacgccgacg acggtgtcga ggacggtgtc
     7321 cgactgcttc gacgactggg ccgtcacccg gccctgcacg agctcgagga gctgtgccgc
     7381 gtagaccggg tcgtgggggg cgtcgtagac ccagcggtgt ccgagggcgg tgtgctccat
     7441 cgtgccgacg agagcgtggt ctgcgctgcc gaggggctcg ccccggtacg tgagcggcac
     7501 ctggtagacc acgggttcgg ggcctgactc gtcggcgacg atgagcgtct cgacgccgac
     7561 ctcaccggcg gggtcgtcca ggcgccaggc cgccagcagg cgcaggcggg gctgctgacc
     7621 cttggcggcg taccagcgct gttcacccat ccagcccgag atgagctcga gcttgctcgg
     7681 gcgcagcgac gcggtcgtga tgatctcagc cacggacgtc acccctcggc gggtccaggg
     7741 cgagccagaa gaagtctcgc gagcccaggg tgatcgtcac ggtgccgtcg tcggcgatgt
     7801 cggggaagcc gccaccaccg aagaggtcgc gggtctccca cccgcgcagc tcgtcgggga
     7861 cggtgaccgt cgtggcctgg ggacgcgagg agaggttgtt cacgcacagc accgccatcg
     7921 ccgagcggtc ctccttgtcc cgctcggacc ggtctacccg gacgaaggac agcacgcggt
     7981 cgttgaccga cgtgcagatc tcgaagtcgc cgagcccgaa caccgggtgt tcgcggcgca
     8041 cggcgagcat gctgcgcacc cagtgcagca gcgaggaacc ggtggccatc tgcgcttcga
     8101 cgttcgtgtg gttgtggtgg tagacgaggg acgtgatgac cgggaggtag agcttgccgg
     8161 ggtccgccgt ggagaagccc gcgttgcggt cgggagtcca ctgcatcggg gtgcggacgg
     8221 catcccggtc ctccaaccag atgttgtcgc ccatgccgat ctcgtcgccg tagtacaggc
     8281 agggggagcc cggcagcgag agcagcaggg cgtggatgag ctcgatctcg ggtcgggagt
     8341 tgtcgagcag cggagcgagg cggcgacgga tgccgacgtt ggcgcgcatc cgcgggtccg
     8401 gggcgtacca gccgtacatc gccgcgcgct gctcgggcgt gaccatctcc agcgtcagct
     8461 cgtcgtggtt gcgcaggaag gtgccccact gcgcgcccgc cgggatcggc ggggtgtccg
     8521 cgagcacctc gatgatcggt gtcgcccgct cctcgcgcag cgagtagtag agcatcggca
     8581 tcaccgggaa gtggaagcac atctggcact ccggcgcctc ctcggtgccg aagtagtcga
     8641 cgacgtcggc ggggggctgg ttggcctcgg cgagcagcac ccggccgggg tactccttgt
     8701 cgaccatcgc gcgcagctgg gcgaggaagg cgtgggtctt ggggtggttc tctccgttgt
     8761 ggccctcctc ctcgtagagg tagggcacgg catccaaccg gaagccgtcg atgccgaggt
     8821 ccatccagaa ccgcacgacc tcgaacatcg cctcgtgcac ggccgggttc tcgaagttca
     8881 ggtcgggctg gtggctgaag aagcggtgcc agaagaactg gcggcggatc gggtcgaacg
     8941 tccagttcga ggtctcggtg tcgacgaaga tgatgcgcgc atcggtgtac ttgtcgtcgg
     9001 tgtcggacca cacgtagaag tcgccgtacg gaccgtcggg gtcgctgcgc gaggcctgga
     9061 accacgggtg ctggtcactc gtgtggttca tgacgaagtc ggtgatgatc cggatgccgc
     9121 gggcgtgggc ctggttgatc agctcgcgga actcgggcag cgtgccgaac tcgggcagca
     9181 cctgcgtgta gtcggagatg tcgtagccgc cgtcgcgcaa cgggctcgcg tagaacggcg
     9241 gcagccacag gcagtcgacc ccgagccact gcaggtagtc gagcttgccg acgacgccct
     9301 ggaagtcacc cgtgccggac ccggtggtgt cgtggaaggc ccgcgtgagc acctcgtaga
     9361 agaccgcggt ccggaaccac tgggggtcgt gccgcagccc gccggtggcg gacgcgctgg
     9421 tcgggaaggc ggtcaacgaa tctcctgcga ggtcagcgac ggtggcgcgg gcagttgggc
     9481 gtccacgaga gcggccgatc agttgatcac caggtcggtc gatcacgcgg ggtgtccgac
     9541 ccgagtgttc cacgtcgcgg ggtgctggcg ccctccccgc gaacacgtct gcgaaaacgc
     9601 ttcgatcccg gtaccgtggg tcggcgcggc agcagggtga ccacatcgtt cctgctctcg
     9661 tcctggctcc gctcaacgtc gaggtccgtg ccacccacca cgcccgctgc tctcttgctt
     9721 cgcacccgag gatcccgctg tgaccgacgc actgaacgtc ccgctcgcct ccaccgagcc
     9781 caccgccgac gaggcgtccg cgacgacgga cgaggccacc accgtggccc cggccgacgg
     9841 cgacgcctac cggcacgaga ccggccccac gtccgagccg acgagcccgc tggcccgcgg
     9901 tccggtcatc tcgccgccgc acaccgtcga gggcttcgtc aaggcgttcc tgcatgagct
     9961 gaacttcggt cagggggtgc cgctcgcgac ctcgtcgcag accgaccagt acctcgcgct
    10021 ggcgcgcacg gtgcggtcct acctcatggc ccgctggctc gagaccgcgc gcaagcgccg
    10081 cgcgtcgcag ccgcagaaga tggtcggtta cctctcggcc gagtacctgc tcggccgcca
    10141 gctcgacaac gcgctgtccg cgaccgagct gcgcgaggtc gcgaccgacg ccatgaaggc
    10201 gtgcggcatc gacatcgagg acctgcgcac gcaggaggtc gagccggggc tgggcaacgg
    10261 cggcctgggc cgcctcgcgg cctgcttcat cgactcgctg gcgacgctcg gggcgccgag
    10321 catcgcctac ggcatccgct acgagttcgg catcttccgc cagacgttcg tcgacggtcg
    10381 tcaggtcgag cagcccgacg cctggctgag cagcggcgac ccgtggggcg tgccgcaccc
    10441 cgagctcgag gtcccggtga acttcggcgg gcacaccgag cggtacgtcg gcgacgacgg
    10501 ccgcgagcgc agccgctggg tgccgggctg gggcgtcatc ggcctgcgct acaactacat
    10561 ggtccccggc taccacaacg gcgtggtcaa cacgctgcgg ctgtggcggg cgcgggcgac
    10621 caacgccttc gacctgcaca tcttcaacgc cggcgactac gccgaggccg tgcgggcgca
    10681 gaccttcgcc gagaacatct ccaaggtgct ctacccggag gactccactc cgcagggcaa
    10741 ggagctgcgc ctgcagcagc agtacttctt cgtggcctgc tcgatcaagg acttcctcag
    10801 ccacacgatg cccaagggct tcgacgtgcg tgagctgccc gagcggatca tcttccagct
    10861 caacgacacc cacccggtca tcgccatccc cgagctgatg cgcatcctcg tcgacgagca
    10921 cgacctcgag tgggacgagg cgtggggcat cacgaagcag tgcttcgcct acacgtgcca
    10981 caccctgctg cccgaggcgc tcgaggtgtg gccggtcagc ctcatggagc ggctgctccc
    11041 gcggcacatg gagatcatct accgcatcaa cgaggagttc ctcgacgacc tgcgcgaggc
    11101 ctacccgggt gacgagctgc gggttcggcg catgtcgatc atcgccgacc accccgagcg
    11161 ctcggtgcgc atggcccacc tggccacggt cggctcggtc aaggtcaacg gggtcgcggc
    11221 gctgcactcg cagctgctca aggacaaggt cctgaacgac ttctcggagc tgtggcccga
    11281 ccgcttcacg aacgtcacga acggggtcac cccgcgccgg ttcatccgcc agagcaaccc
    11341 ggccctcacc gagctcatca ccgagaccat cggcaagggg tgggtcgccg acctcgaccg
    11401 gctggccgag ctccaggcct acagcgacga cccggagttc cgcgagaagt tccgcgccgt
    11461 caaggcggcg aacaaggaac ggatcaccga cgtcctcgcc cggcgcgacg gcatccagct
    11521 cccgcagggc cacatgctcg acgtcatggt caagcgcctg cacgagtaca agcgccagtc
    11581 gctgaagatg ttgcacatcg tgacgctcta cgaccgtctc gtctccggcg aggtcgaccc
    11641 ggcgagcatc accccgcgca cggtcgtctt cggcgccaag gcggccccgg gctaccacat
    11701 ggccaaggag acgatctacc tgatcaactc catcgccaag accgtcaacg ccgaccctcg
    11761 cgtgcagggt cgcctgttcg tcgccttccc gccgaactac aacgtcaccc tggccgagaa
    11821 gctcatcccg gcggctgacc tgtccgagca gatctcgctc gccggcaagg aggcctcggg
    11881 cacgggcaac atgaagttcg cgctcaacgg cgccttgacg atcggcaccg acgacggtgc
    11941 gaacgtcgag atccgtgagc tcgtcggcga cgagaacttc ttcctgttcg gcatggacga
    12001 gcccgcggtc gccgaactgc aggcacgcgg ctaccgcccc ggtgacttct acgagtcgga
    12061 cgcgaatctc aagcgtgcga tcgacctcgt cgcgagcggt cacttcaccg acggcaaccg
    12121 ggatgccgtg agcgcgttcg tcggcgatct gctctccaac gaccgcttcc tggccctcgc
    12181 ggactacgcg tcctacctcg aggcgcagga ccgcgtcgag gcggcctact ccgaccagga
    12241 ggagtggtcg cgcaaggcca tcctcaacgt ggcccggacc ggcttcttct cctcggaccg
    12301 atcgatgcgc gactacatcg accgcatctg gcacatcaag accaacgcct gaggcagaca
    12361 acgccgcacc cccgcacgcc gatcacggcg tgcgggggtg cggcgttcgg ggaggagctc
    12421 agctgcggcg gacgtggatg acgtgcaccg gcgtggtctc cgggcccaga cgcacgaagg
    12481 ggtgctcacc ccaggtgaac tgctcgtcgg tcagcaggtc gtgggcgacg aactcgtcac
    12541 cccaggacag gcccagcgcc ggcaggtcca ggtgggcgtg ggtgccacgc gtggagtgcg
    12601 ggtcgaggtt ggcggccacg aggatgacgt cctcgccgcc gtcggcgtag acccgccgct
    12661 tgctgaacac caggatgttc tcgtcatcgg cgctgtggaa ggtgatgttg cgcagccagt
    12721 gcagcgcgcc gtggttggcc cggatggtgt tgatctgggt gagcagcccg gccagtgact
    12781 gaccctcctt ggggccaccc acctcgtagt cggcccagcg gcggtccttg tactcgtact
    12841 tctcgttgtc gatctgctcc tcggcgccgg ggcgggccac gtgctccatg agctcgtagc
    12901 cggcgtagat gccgtaggtc ggcacgagcg tcgccgcgag caccgcacgc accacccagg
    12961 cggcgcggcc accgaactgc atgtagggcg tgaggatgtc gtgcgtggtg ggccagaacg
    13021 agggccgcat gtacgtggcg gcctcaccgg cgagctcggt gacgtactca cgaagctccc
    13081 acggggtgtt gcgccacgcg tagtaggtgt agctctgctg gaagccgatc ttgcccaggg
    13141 tgtgcatcat cgccggcttg gtgaaggcct cggcgagcca gatgacctcc gggtggtcct
    13201 tcgcgacgtc ggcgatgatc cactgccaga actccaccgg cttggtgtgc gggttgtcga
    13261 cgcggaagat cttcaccccg tggtcgatcc acacctggac gacccgacgc acctcggcgt
    13321 agatgccggc ggggtcgttg tcgaagttga cggggtagat gtcctggtac ttcttcggcg
    13381 ggttctccgc gaacgcgatg gtgccgtctg cgcgggtggt gaaccactcc gggtgctcgg
    13441 tcacccacgg gtggtcgggg gagcactgca gcgcgaggtc catggcgacc tcgagcccga
    13501 gcccctcggc gcgggcgacg aaggcgtcga agtcggcgaa ggtgccgagg tcggggtgga
    13561 tcgcgtcgtg gccgccgtcc ttggagccga tggcgtacgg cacacccggg tcctccgggc
    13621 gggcgtcgag ggtgttgttc gggcccttct tcgccgcttg gccgatcggg tggatcgggg
    13681 tgaggtagat gacgtcgaag cccatcgcgg cgacggcggg cagccgctcg gccgcggtgc
    13741 gcagcgtgcc ggtggtccag cggtgctcct tctcgtcgta ggtcgcaccc tcgctgcgtg
    13801 ggaagaactc gtaccacgct ccggagagcg cgagctcgcg ctccacgagc agcggcagct
    13861 cggccgacgg gctgacgaag tcgcgcagcg ggcgggcgga caggacgtcg gcaaccgccg
    13921 ctccggtgcc cgcacgcagt cgcacggcgg cggatgccgt ggtgtcccgc aggccgcgga
    13981 tgccgtcggc gagcgcggcc ttggcggcct tgtcgcgctt gacctccttg cgggcgcgct
    14041 cgagaacccg ggcgccctcc tcgagcatca gctcggtgtc gacgtcggcc tgcaccttga
    14101 tcgtcgcgtc gtggacccag gtgccgtacg ggtcggacca gccctcgacc cggtaggacc
    14161 accagccggt gcggtcagcg gcgacgaccg ccgaccacgc gttcaggccc gggttggtgc
    14221 actccatcac ctggacgtgc tccacgccgt ccgggtcggt cagcaccagc gaggcgttga
    14281 cggcgtcgtg gccttcgcga agaaccgtcg cggtgacggt gaactcctcg ccgaccaccg
    14341 acttcgcggg gcgggcgccg tggtcgacgc acggggcgac gtcgagcacg gggattcggc
    14401 cgatcgggtg ctggggggcg ctggtgcgcg ggggagcggt cacgtcagca acctaccggc
    14461 cccatagggt tgcgcgcatg agtcgtttcg tgtgggccgc gtggggcctg ctggtcgtcg
    14521 tcctggatct cccgcttttc ggctgggacg ttctccccga catcgtcggc tacctgtggc
    14581 tggtcgtcgg tctcggcggt gcgacccaca tggccttcgc ccgcgcccgg gctgccgcgg
    14641 ctgccggtgt gccggttgcc ctcgtcacgg gcacaccgct gttcgtcggc caggacggca
    14701 tcatctgggg tgcggtcttc atcgagatcc tgctgacggt gctgatcctg caccagctcg
    14761 ccaccgccat cagcgacctc gcccccgacg gcgaccagga cgcgcgacgg tgggcgcgcg
    14821 gcatccgcat cggggcccct gccgcaggcg tcatccggct catcggcctg gtcgggctcg
    14881 cgacgtcgct cggggcgctc tacgcgctcg ggtacctcgg gctcgtcgcc gtcggcatcg
    14941 tcacggtcgt gctgatgcac cgggtcaacc gggccggctg gctcgacctc gcaccaccgg
    15001 acccggcctg accccttccc ggaaccggtc gagagtagac gaatcgccgc cacccccaag
    15061 gggatgacgg cgattcgcct actctcgacc gggctggagg ggtgcctcag cgcaggaacg
    15121 ggatgttgcg gtcgagcagg ccacgcgact tgagctcggc gcgcagcggg atctcgtcgt
    15181 cgatccagcc gctctgcttg atgccccaga acgacgcgtg gtcggggccg tagccgagca
    15241 gctgctggtg cagcggcgac agacccttga accactcgga cgactggtcg gccacggcct
    15301 cgtcgagcgg gcggatccag cggtaggtgt agccgatgaa catcagcttg cggatgcgct
    15361 cgctcttgtt catcgagcgg ctgtgccaca ggcggcggtc gaagatgaac gcgtcaccgg
    15421 ggttgagcag cacctcgacg gcgccctcgg gctcgatgat cgggtcgccc tccttctcgg
    15481 ggcggccggc gagcgtgttg ttcttgtggc tgccggggat gaccttggtg ttgccgtaac
    15541 cgggctcgga caggtcggtc agggcgtagc agatcttcgt catgaacatc ggacgcacgt
    15601 ccatgtcgat gtcggagttc tgccggtagc cgtcctggtg ccagttccag aagggcggct
    15661 cgtccttcgg ggggttgacg tcgatgtggt tgtggtgggt gtagatgttc cagcccatcg
    15721 ttccccagat gtaggggaag gtctgcggcc agttcagcag ctcgacgaag cgcgggtcct
    15781 tgtgcatcca ccccatgacg tggatggagc ggtcggggcg cagctcgccc gcggcctcct
    15841 cctcgccgta gatgcggtcg acggcatcct cgaagtaggc gcggtccgcc tcggggagcg
    15901 cgttcgggat gacgacgaag ccgtcgcggt cgtactgctc gcggatcgcc gggtcgaggg
    15961 gctcgaaacc ggtgggtgcg gagacggttg cggtcatgcg gtgatgctcc caactggtgc
    16021 cgacggttgc gggggcacca tcccgcggca gcccgtcgcg acggtcgcaa ctctcgctgt
    16081 cgtcacggcg ggcgcctgct tgccccaagc ctaagccgag ggcgtcaact cacgtcggat
    16141 gccagcctca actgacgtcg gatgccgcgt acacgcgaag gctcgtggcg ccgaccgtga
    16201 ccggcccggg aaccacgggg tcgccgccgt cgcgcggtcg cgaccacgtg ctgtcccaca
    16261 gcaggcggta ggtcgtgacc ccgggcgcct cgggcagcgt cacctcgacg tcctcgacac
    16321 cgccgttgac gacgagcagc gcagagtccc agcccatcca ggcgcctgcg acgtacagct
    16381 gcaccaggcg gctgccctgg gtccaccggt cgcccatcgg ggtgccgtcg gcggcgtacc
    16441 actccatgtc gcgagtgccg tcgtcgtgga cctgccgccc gagcgcccac acccgctggc
    16501 gcagcaccgg aagggcctgg cgcacccgga tcaggtggct ggtcgtgtcg aggaggtcct
    16561 gctgccacgg ctcgaggtcc cagttgatcc acgaggtgcc gttgtcctgg cagtaggggt
    16621 tgttgttgcc cccctgcgag cggcccagct cgtcaccggc gttgatcatg gggacgccgg
    16681 tcgacagcag gagcgtgccc atgaggttgc ggatcgccag ggttcgggcg gcgatcacct
    16741 gcgggtcgtc gctcggcccc tcggtgccgt ggttccacga gccgttgttg tcgctgccat
    16801 cgcggttgtc ctcgccgttg gcctcgttgt gcttcgtgtc gtaggccacg aggtcggcgg
    16861 tggtgaagcc gtcgtgggcg gcgacgaagt tgatggatgc cgtcgggcca cggtcgcggt
    16921 gaccgaacag gtcgcgtgaa cccgccaacc tcgtggcgag ctcctggagc ccgtgcccgc
    16981 gacggtggtg gcgctgggac cggacgtcgc ccacccagaa gcggcggacg gcatcccggt
    17041 agcggtcgtt ccactccatg aacggaggag ggaactggcc ggtgcgccag ccgtgcatgc
    17101 cgacgtccca cggctcggcg atgagcttga cggccgagag cacggggtcg gtgcgcagcg
    17161 ccacgagaaa ggggtggtcg gggtcgaagt cgtccccgcg accacggccg agcgccacgg
    17221 cgaggtcgaa gcggaagccg tcgacgtggt actcctgcac ccagtagcgc agcgagtcca
    17281 gcaccatgcg acagaccacc gggtggcgca ggtcgagcgt gttgccacac ccggtgacgt
    17341 cgatgtcgcg gccccgctcg tcgaggcggt agtacgcgcg ctggtccagc ccgcgccacg
    17401 acaacatggc gcccgtgcgg ccctgctcgg cggtgtggtt gtagacgacg tcgaggatga
    17461 cctcgatgcc ctcgccgtgc aggagcttga ccatgccctt cagctcgtcg acggcaccct
    17521 gcgggtcctt cgcggccgca taggcgccat ggggcgcgaa gaagccgagg gtgttgtagc
    17581 cccagtggtt ggtcagcccg cggcgcacca ggtgcggttc gtgggtgaac gcgtgcacgg
    17641 gcagcagctc gacggccgtc accccgaggg aggtgaggtg ggccaccgac gcggggtggg
    17701 ccagcccggc gtacgtgccc cgcagctcgg ggggcacacc ggggtgcagc gcggtctggt
    17761 tgcggacgtg ggcctcgtag atgacgctct cgctgcgtga gcggttcggc gcgacgtctc
    17821 cctcccagtc gaagcggtcg tcgaccacga cgccgcgcgg catgtggccg cggctgtcga
    17881 ggtcggagat gagctcgccg tcgccgtgcc agcgaacgtc gacgacgtgg ccgtagacct
    17941 ccgcacccca gcggaccttg ccctcgatcg ccttcgcgta ggggtcgagg agcagcttgg
    18001 ccgggttgtg ccgcagcccc tggtccgggc tccacgcgcc gtgggcgcgg aagttgtagc
    18061 gctgcccggc cgtgatgccc gggacgaagg cgaaccacca gccgtgtgcc cgctccagca
    18121 gggggatgcg gcgctcgctg gtgccggcct tgtcgccggc gtcgaacagg cacagctcga
    18181 cggcgtcggc gtggccggca tagaccgcga cgtcggcgcc gccctcgacc agcgtcaccc
    18241 cgggccgagg ggcgaggtcg cgggtcatcg ggccgggcgg gatggtggtg gggtgcgacc
    18301 agggcgacat ggaggggagc ctaatgagtc gttacggtgg acccatgacc gatcccacgt
    18361 cgctgcccaa cttcccgccg cccgccgacg agcaccccct gcgcggtcgg gtcctcgaca
    18421 tcctcgtcga cctgggcctg gcgcccaacc tcgacaccga cggcgacgtc gccttcaccg
    18481 tcaacgagca gcagctgttc atccgctgca ccgagggtga ggtcgagatc atgcgcgtct
    18541 tcggccagtg gcagatccag gaggagatgc tcggcgaccg cctgaagctg cacgagacgt
    18601 gcaacgagct caacctcaac atgaactgcc tcaagagcgg cgtcgccggc tccaccctgg
    18661 tcgtcacgag cgagcacctc gccaccccgg gtgccgacgt ctcctcgctc atccaggtct
    18721 cgatccaggt catcctctcc ggggtgcaca tctggcacca gcgcctgctc ggcatcgacc
    18781 ccaacgcgcc cgagtcggat gccgggagcc cgtcgtgacc gacctcgtcc gcctcgaggt
    18841 ccttgacggc atcggcacga tccgcctgga ccggccgccg atgaacgcgc tcaacgccga
    18901 gatccagcac ggcctcatcg ccgcgtgccg cgaggcggcg gagcgcaagg acgtcgccgc
    18961 ggtcatcgtg tggggcggcg agaaggtgtt cgccgccggt gcggacatca aggagatgga
    19021 gaccatgtcg tacaccgaca tggtcgacca ctcggccctg ctccaggact tcacccggtc
    19081 gctggcccag cttcccaggc ccaccgtggc cgccatcacg gggtacgcgc tgggcggcgg
    19141 gtgcgaggtg gcgctcgcgt gcgacttccg catcgccgcc gacgacgccg tgctcggtca
    19201 gcccgaggtc tcgctcggga tcatccccgg tgccggcggc acccagcgcc tggcccggct
    19261 ggtcggcccg tcacgcgcca aggacctcgt cttctccggc cggttcgtca aggcgcccga
    19321 agcgctggcg atcggcctcg tcgactccgt ggtcccggca tccgaggtct acgagagcgc
    19381 gcggagcatg atggcgcgct acgtcggcgg cccggcgtac gccctgcgcg ccgccaagga
    19441 ggccatcgac cgcgggctcg aggtcgacct cgacagcggc ctggagatcg agcggatgct
    19501 gttctccgcg ctgttcgcca ccaaggaccg ggcgatcggc atgcgccact tcgtcgacaa
    19561 gggccgtggc ccggcaccct tcgaaggcgc ctgagaccgc ggtggccgcg tcggcgagtc
    19621 aggcgccgag ggcatcctga agcgcggttc gctttgcggt gacggcggcc ccgtgctcgt
    19681 cgcgcacgct gccccagagg tcgtcgagca gggtgtcgat gcgtcggggc acctgcagcg
    19741 agtccttgcc gtagtgcgcg atctcgtcga gtgagaggtc gagcagccga gcgaaggtcg
    19801 tcggcaggtc gaggacgcgg accgtgccgt cggcatccac gaggtggggt gagcgatctg
    19861 ccctggatcc cgcgacccgc aggatgcggt ggagctcgtc gagcacctgg actgccgtgg
    19921 tggggtcgtt gatgccgggg gagagggcac gttcggcgat gtcgaccagt tgccgcaccc
    19981 cgaactcggg atcctgcggc atcgagcgtt ccttgccgag cccgaacgag ctgcgcacct
    20041 tgtcgatgac gtcatcgtcg gcgaaggccc cgtggacgac cgcgagcgat tgcccccgat
    20101 ggcggacctc gcccacgcgg gtggtcagct cgacgacgac cccggcacgc cgtgccgtct
    20161 ccacgaggtc gatgtagcgc acgcgctgca ccacgccgtg gcccgagccg gccgacacgg
    20221 tgacggacgg gcccggcgcc tgcgaccagg acggcgaggg agtcgaggtg tcgccccact
    20281 cgtcgtcccc ctgctgcagg gcacgcacgg tctggtccgc gacccggtcg atcaccttgg
    20341 atacctggat cgacacggtg atgtggtgga tgaaggcgaa gaacaacgcc acgctggcca
    20401 gcacgagcag gaaagccacc gtgaccgaga gctgggggac gaaggggtcg tcgccgccac
    20461 gcacgtcgcg caggacgatg agggcgaaca cgaaggacga ggtgaagaca cccagggtca
    20521 cctgggtgat gcggctgctg aggaagctgc cgagaaggcg cggggtgaac tggctgctcg
    20581 ccagctgcat gacgaccatc gtgatcgaga agacgagccc ggtgaccgag atcatcgcgc
    20641 tcgcgatggt gctgagcagg ctgcgagcgg actcggcccc accgccgaag aggtagggca
    20701 gatcctcggt cagtgcctcg tcgacgcgag gcagcaccac gcccgccacg acggcaaggg
    20761 cgcagatcgt ggacggcagc acccagaacg gggcccacag ccgctcccac cagccgatcg
    20821 tctccgggtc gccgaccggc ccgacggtgc ggcgggcccc gcctggttcc gtgtccgtgg
    20881 tcctgaccat ctgcgtgatc ctgccagcga aacccatcca tccggcgccg acgtgcgagt
    20941 gagtggatcg cccgtcggcc ggccccgggt atgcgggggt ggcgggcatc acgccgatgc
    21001 gatggtgaac aagcgctcac cgcctgagag aatcgacgcc acgcccgagc cgaacgcgcg
    21061 ggcacctgtc acctgttgac accagcccac ttcggctgac caagccacca gcaagagatg
    21121 aggacgaggc acgcatgaac atcgtcgtct gcgtcaagca cgtgccggac gcgcagtccg
    21181 agcgcacctt ccgtgagtcc gacagcacca tcgaccgcga aggcgtcgac ggactgctct
    21241 ccgaactcga cgagtacgcc gtcgaagagg cgctcaagct cgtcgaggcc gctggcgagg
    21301 gtgaggtcac cgtgctgacg gtcggccccg ccggcgccgc cgacgcgatc aagaaggcgc
    21361 tgcagatggg ggcgcacgcc ggcatccacg tcagtgacga ggccatccac ggctccgacg
    21421 ccgccgccac ctcgctggtg ctcgccgagg ccgtgaagaa ggccaccggt ggcaaccccg
    21481 agctcgtgct caccggcatg gcctccaccg acggtgtcat gggtgtcgtc ccggcgatgc
    21541 tcgccgagcg cctcggcctg ccccaggtga ccttcgcctc cgagctcacc atcgccgacg
    21601 gcacggtgac catccggcgc gacggcgaca cggcatccga gacgatcgtc gcgagcctgc
    21661 cggccctggt ctcggtgacc gaccagatca acgagccgcg ctacccctcg ttcaagggga
    21721 tcatggcggc caagaagaag cccgtggagc agtggggact ggctgacctc ggcatcgacc
    21781 cctcgctcgt cggtctggat gccgcgtgga cgaccgtcgt gtcgttcgcc aagcgtccgc
    21841 cgcgcgccca gggccagatc gtcaccgatg gtggcgacgg cggcacccag ctggcggagt
    21901 tcctcgccgg ccagaagttc ctctgacccg gccgacgacc agacaaggta ggacaccccc
    21961 atggctgaag tactcgttct cgtcgaccac gccgacggac gcctgcgcaa ggcaaccgct
    22021 gagctgctga cgatcgcccg ccgcctcggc gagccctccg ccgtccacat cggccccggc
    22081 gccgaccgcg cccaggtcac gctcgccaag tacggcgcgc acaaggtgta cgtcgtcgat
    22141 gacgccgacg ccgccggctt cctcgtggcg ccgcaggccg agatcctcgc ggcgctggtg
    22201 gagaaggtcg ccccggcggc cgtcatcatc ccgagcaacg ccgccggcaa ggagatcgcc
    22261 gcccgtctgg cgatcaagac gtcgtccggc ctcatcaccg acgccgtcga cgtgcgcgcg
    22321 ggggagggcg gcatcgagac aacccagtcc gtcctcgccg gctcgtacac cgtcaccgcc
    22381 aaggtcacca cgggcacgcc gatcatcgcc gtgaagccga actcggttcc gctggaggcc
    22441 tccccggccc ccggtgtcgt cgaggccttc gccgtcacgg tgagcgacgc cgccaaggcg
    22501 gcgcgcgtca ccgacagcca gcccaagaag gcaaccggcc gtcccgagct caccgaggcc
    22561 gccatcgtcg tctccggcgg gcgcggaacc ggcggtgact tctcgcccgt cgaggagttc
    22621 gccgacagcc tcggcgcggc cgtcggggcg agccgagcag ccgtcgacgc cggctggtac
    22681 ccgcacgccc accaggtcgg ccagaccggc aagcaggtct cgccgcagct ctacgtcgcc
    22741 gccggcatct ccggtgccat ccagcaccgc gccggcatgc agacctcgaa gacgatcatc
    22801 gccatcaaca aggacgagga ggcgccgatc ttcgagctcg tcgacttcgg agtcgtcggc
    22861 gacctgtttt cggtgctgcc gcaggccacg gccgaggtca agaagcgcaa gggctgaccc
    22921 aggcgcagat ctggcaccac gaaaccaccg ctcacgaggc ggtctgccgc gcgagcggac
    22981 gatgaagggg cgaccgaatc ggtcgcccct tcggcgttcc cgnnnnnnnn nnnnnnnnnn
    23041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23101 nnnnnnnnnn nnnnnnnnnn nngatggcgg ggccttgttc ccgtcgctga tcgttactcc
    23161 gctcgggcgt tgctgcatga gcgcaccagc ggagcaaggg cgcacatccg gagcaaggat
    23221 ctgcgtcggc ggcgcgaccg cttgcgctga cacggtttcc gagccgcggc gcgcccgctg
    23281 actgccgccg ggggatgccg tgcggtcggg ggaggccgcc gccgggggat gccgtgcggt
    23341 cgggggagtc cgctgcccgg tccgaaacgt agaatcgccc gttgtgaccc actacctcga
    23401 ccatgcggcg acgacaccca tgctgcccgc cgcggtcgat gccctgctcg aggtctcccg
    23461 ccaggtgggc aacccgtcgt cgctgcacac ctccggtcgg gccgccaggg cggtcgtcga
    23521 ggaggcccgc gagcgcatcg gcgcggccct cggagcccgg ccgtcggagg tggtgttcac
    23581 ctcggggggc accgaggccg acaacctcgc cgtcaagggc acgtggctgg cccgccgggc
    23641 gaaggatccg tccgtcaacg gcgtcgtggc cagctccatc gagcaccacg cggtgctcga
    23701 ccccgtcgag tacctcgagg actcccgcgg ggtccccgtg acctggctgg aacccgaccg
    23761 ctgcgggcac ctcgcccccg acaccctgcg cgcggccctg gctgcgggcg gcgtcgcggt
    23821 cgcgagcgtc atgtgggcca acaacgaggt cggcacgatc cagccggtgg ctgagctcgc
    23881 cgcggtcgcc cgcgagcacg gggtgccctt ccacaccgac gccgtgcagg cgatcgccca
    23941 cctgcccgtc cgcttcgacg acagcggtgc cgacctgatg accgtcagcg cccacaagat
    24001 cggcgggccc gtcggggtcg gtgccctgct ggcgcggcgt gacgccccgc tcgtgcccat
    24061 cgcccacggt ggtggccagg agcggcaggt gcgcagcggc accctggacg cggccggcat
    24121 ccactcgttc gccgtggccc tggaggagac cgtcgcccac cgagacgtcg aggccaagcg
    24181 cgtgctcgcc cttcgcgacc ggctcatcga gggggcgctc ggcctcgggc tcggcatcac
    24241 cgtgagcggt tgctggaagg ccggcgacgc gaccaagcgc ctgccgggca acgcccacct
    24301 gctcgtgccc gggtgcgagg gcgactcgct gctctacctc ctcgacgcgg ccggggtcga
    24361 gtgcagcacg ggttcggcct gccaggccgg ggtaccgcag ccgagccacg tgctgctcgc
    24421 catggggcac accgaggcgc aggcgcgcgg cgccctgcgg ctctcgctcg gccacacctc
    24481 gaccgaggcc gacgtcgacg cgttcctctc cgccctgccg ggtgtcgtcg agcgggctcg
    24541 acgggcgagc gggctgatct gatgcgggtc gtcgccgcga tgtcgggcgg ggtcgactcg
    24601 gccgtcgccg cctcgcgcat gatcgaggcc ggccacgagg tcgtcggcgt ccacctggcc
    24661 ctctcgcgct cggccgcgac gctgcgtgag tccgcccgtg ggtgctgcac catcgaggat
    24721 gccggggacg cgcgtcgcgt cgcggaccgg ctcggcatcc cgttctacgt ctgggacctc
    24781 gcgagccgct tcgagcgcga cgtcgtcgag gacttcgccg ccgagtacgc ggcggggcgc
    24841 accccgaacc cgtgcctgcg ctgcaacgag cggatcaagt tcgccgggct gctcgacaag
    24901 gcggtggccc tggggttcga tgccgtcgcc accgggcact acgcgcagat cgtccagggc
    24961 ccgacgggtc gcgagctgca ccgggcggtc gacgcagcca aggaccagtc gtacgtgctc
    25021 ggcgtgctgg acgccgacca gctggcgcgc tcgttcttcc cgctcggtga ctcgacgaag
    25081 acccaggtgc gggccgaggc tgcggagcgt gggttcgcgg tggccaagaa gccggacagc
    25141 cacgacatct gcttcatccc ggacggcgac acccgtgggt ggctgaaccg gcggctgggg
    25201 gagcggccgg gcgagctcgt cgacgcggtc tcgggtgccg tcgtcgggtc gcacaccggg
    25261 gcgcacggct tcacggtggg gcagcggcgc gggctcggca tcgaccggtc ggcgctcgac
    25321 ggcgacccgc gctacgtcgt cgagatcgac gccccgagca accgggtcgt catcggcacg
    25381 gccgacctgc tcggggtcga cctcatcgtc ggtgaccacg tgcgctggtg cggcccggcc
    25441 cccggcgagc gggtcgcgct cggcgcgcag gtacgggcgc acggcgagga ggtgcccgcc
    25501 acggccgagg ttcaggccgg gccgtcgccg accgtccggg tgggcctcga gcgccgggtg
    25561 cgcggtgtcg cgccggggca gtccgtcgtg ctctacgagg gcacccgggt cgtcggctcg
    25621 gcgaccatca gcgagaccgg acgcgcctga ccacctcgtg tcacggcatc cgtgatcttt
    25681 ctgcggagtc aggtgcaccc cgccgactca ggtccgggta ggaccctact cggcgaagtg
    25741 ctcccgaccc cgcggccagg agcggaatag ttgatgcgtc aactagcgtt gggagcagta
    25801 ccctccgaaa ccggggaacc cagaagcgca aggagctgat caccatgcca gccgtgaccg
    25861 tcgagaacat cctcacgctg ccccgcgtgt cgcaacccgt gccggatgcc gtgacgcgcc
    25921 ccgtgcgctc ggtcacgaca gcgccgagcg gcttcgaggg cgaagggttc cccgtgcggc
    25981 gagccttcgc cggggtcgac ctggcggcgc tcgacccgtt catccacatg gaccagatgg
    26041 gtgaggtcga gtacgccccg ggcgagccca agggcactgc ctggcacccg caccgcggct
    26101 tcgagaccgt cacctacatc atcgacggca ccttcgagca ccgggacagc cacggtggcg
    26161 gtggcacgat caccaacggt gacacccagt ggatgaccgc gggctcgggc ctgctgcaca
    26221 tcgaggtgcc gcccgagtcg ctcgtgctgt ccggtgggct gttccacggc ctccagctgt
    26281 gggtcaacct gcccgggtcg atgaagatgt cgaacccgcg ctaccaggac atccgcggcg
    26341 gcgaggtggc cctgctctcc acgcacgacg gcggcggcct cgtgcgcgtc atcgccggcg
    26401 aggtcgacgg ccaccagggc cccggcatca cccacacccc gatctcgatc gtgcacgcca
    26461 ccatcgaggc cggtggccag ctgcgcctgc cgtggaaccc gcagttcaac gcgctcgtgt
    26521 acgtgctcaa cggcctcggc acggtcggcc ccgaccgtcg cccgatccgc cagggacagc
    26581 tcgtcgtgct cggtgccgga gacgtcatcg aggtcggtgc gaacgagcag caggagagcc
    26641 gcagccccag cctggacctc tacatcatgg gtgggcgccc gatccgcgag cccgtcgcgg
    26701 cctacggccc gttcgtcatg aacacccgcg ccgagctggt ccaggcgttc gaggacttcc
    26761 aggcgggcaa gctcggcacc atcccgtcgg agccgcaccc cgggcacagc ttcagctgag
    26821 cacccttcag ccggggcggc ggccgagcat gatgaccgtc gccacccccg aggcgcgctc
    26881 gccgatgcgc tgcgtcgcga gcgggcgctc ctcgggtggc agggccacga gatccatcgc
    26941 atcgagccac acctggctgc gccacgactg cgccagcgtg ccgaaggccc actcgtcgag
    27001 cgggcccatc agggcgtcga gcatctcggc cagaggtgac cactgggcga cgacgtcgcg
    27061 ctgcacctcg tcagtggtct cgtcgatgat cgtgttgacc agttcgtggt cctcgtgacg
    27121 atggcggcgc tgctcctcct cgagggtgcc ccagtcgtcg agcacgtcga ccagggcggg
    27181 ggcgaggtcg tggttgatgt gggcgttgat gccgagcatc agcgcctgga gcgggtggca
    27241 gccagggtcg gagcacgcgt cgagggcgcc gcgccagatc tccgggcacg tccgttcggg
    27301 caccggggtg acggtcgcct cgacgccgtc cggggtcgtc gtgacggtcg tgaccgtggt
    27361 cagggtgaag tcgcccgacc ggccgtatgc ctccacggcg tcgaagtagt agtcggcgaa
    27421 ccggtcgagc agacgcgtca cccacacccc gtcgtggaag gccccgttcc gcacgcgctc
    27481 gcgcacccgt ctcgtcatca cgaggtagca ctcggagaac acggcccgac ggtccccgga
    27541 cgccgtccac acctgggtca tcgactccag ccgtcgttcg gcgctcacca cgtcgcccat
    27601 gggtccaacc gtaggggcca cgcggtgcgc tcaggtcgct tttcggcccc tgcccctcgg
    27661 cccggcggct gccagctcgc cgcgagccgc gagggccagc tcgcggacgc gggcgaagtc
    27721 ctcgtcggtg tacacgccct tgacccccga gatcgccgca aggcgcatcc cgtgccggag
    27781 cccgagctgg tagatctcct tgaccttctc ccactcgatg gcccgaccga cggtgaaggt
    27841 ctcgtagcgg ccctcgagcg cgagggcgac cgtctcggcc aggcaggcgt agacgacgcc
    27901 ggtgggcagg ccgatgtcgc gcatccgcac ctcgccgggc agctcgatct cgcccgactc
    27961 cacgacgagg acgtcggggc gtcgggcgac gtcctcggcg gacaggtcca gcggcctcgc
    28021 gacgtcggtg atgacgcagc cgggcttgac gtccatgatg tcgagcacgc gcttgcccgc
    28081 acccgaggtc gccgtgacga tgacgtccat gtgccgcagg tggtcgccgg gggtggcggc
    28141 agcgtgcacg accgcgcgtg ggtgctcgcg ctcgatgtcc ttcttgaggg cgagcagctt
    28201 ggcggtctcg ggggagacca gccacagctc gtcgctggcc agcgcgagca gccgggcgca
    28261 gaccgagccg atggcaccgg tcgcccccac gaccatggcc cgcccccgga tgacgccgtc
    28321 gtcgtcgcgc tccagcaggc ccagccggtc cacggcatcg tgggcagccc acagggcgcc
    28381 ggacgcgctg tagctgttcc cggtggtgat cgggagggac gccttgcgcg ccaccgtcac
    28441 cccggcatcc ccgacgacct tggtgaaggc cccaagaccc atgacctgcg cgcccagccg
    28501 gcgcgcgagc tccgcggcct cgaggagctt gtcgtaggtg aactcggggt cgtgggcgag
    28561 gatctccttc ggcgtcccgc ccaccgtgat cagccacccc tcggcctcgg cgccggtcgg
    28621 ggacctcacc cccgtgacgt gcccgtagac gaaggggggc aggtacgcca tccccttctc
    28681 gacggcatcc atgacgaccg gcggggagac cttcgcgacc gtgccgaggg gctcgacgtt
    28741 gcggaagaac ttctgcgaca gcgggtggat gacgaacgcg aacctgctct tgcggcgcgg
    28801 gccgttgggc tgcaggaccc gcggctcaag gccggccgcg acgatcatct cgaggaggtc
    28861 gtcgtcgctc aggcgttcgg gggcgccgcc cggggcccga acggccgcac gcatcatcgc
    28921 ctcgagggtg gcgacgtcga cggtgctctc gaaaggctgc ggggtgctgt cgagcaccag
    28981 gtcgacgtcc cgggacgcga ggtcggccag ctgcgcgtcg gtgaccgccg aggtgatgac
    29041 ggtcttgccg tgcaggtccg ggaagccgaa ggtcaccagc tcgtggtacg tcgcgacgac
    29101 gacgtcgcag tcccgggggc gctggcgcag ccatgagcgg ttgaccagct cgcccggcgt
    29161 cagcgagtcg tcgcccacgg cggaccgcac ccggtccacg gggtgccacg cgaggcgcgc
    29221 gacccggccg agcaccggga cgcccccgag ccggcgcccc ggcccggcgc ccaccggcgg
    29281 cgcctcgagg tggaggttgt cggtgtactc gcgcagcaca cggaccgtgc gcgcgtggtc
    29341 gctgccgagc acgacggtgc gggcgttgct gaagtagccg ggcatctcgt actggacccg
    29401 ccgcacggcc cactcctgga gcacctcgcg cagccggtgc ccgtcggtgg ccgtgccgcc
    29461 gaccgccgac gcgacgagcc gggccgtcgc ctcggcgggg tcgtggtcga gcagcccggt
    29521 ggcggccgcc tcggcgcccc cgttcaccgc cacctggtcg gcgagctcgg accagcgccg
    29581 cacgagcgcc tgtgccgtgt gcacgtcgcc gtcggtgccc agccgcagca ggcggtagcg
    29641 ccggtcgagg aaccgcaccc tgaggtcgtg gtcgcgcgcg gaggagccga ggctgacctc
    29701 gacgacgagc agctcggggc ctgctcggcc gtctgctccg tgggcggctc ggcggggtgt
    29761 cgtggcgggc atgtgcgtcc tttccgggaa cgactcaggt catcgcgtgc gcgtcgaact
    29821 ccgggtgggc gcccatgaag tcgagcagcg tgtcggcgta gctctcgaac gcggggcagg
    29881 tcagcccgga cgccgcgagg tcggtggtcg tggtggtggt gtcgtacgtc gtcggcgagg
    29941 cgaagtagtc cagcgcctcg gcgggcagcc ccagcagggc ctgcgctccc ggcacgtggt
    30001 cgacgagggc gtggacgggc gcgagcggca gcggcaccca cacgacccgc ttcccgaggt
    30061 gccgggcgaa ggcggtcacg acctcccgga ccgtcggggg gtcggggtcg acgagggcgt
    30121 aggtgcgacc ccgcgactcc tccagcaccg acagctcgtc catggccccg acgacgaagt
    30181 ctcgcgggac gaggcacacc cggacccggt ccgcgtcccc gagggcgggg acgacggcca
    30241 ccgagagctg gcgcagcagg aaggtcgcga ggaagtaggg cccgtcgtac ttctgggtct
    30301 cacccgtcgc cgagtcaccc acgacgatgc cggggcggta gacggtggcg ggcaggccgc
    30361 tggccatcgc tgcccgcacg agcacctcgg cgtcgtactt ggtccgttcg tagtggttgc
    30421 ggaaggcctg cccctcctcg aggtcgtcct cggcgaagca gccctcgtag cgaccgctga
    30481 cgtagcaggt gctgacgtac tggaggcgcc gcaggtcgcg aaggccccgg cagaactcca
    30541 ggacgtgccg ggtgccctcg acgttgacgc ggcgcgcgac ctcgggggcc acggccaggt
    30601 cgtagacggc ggcgaggtgc cagacctcgt cgacgtcgtc caggcccgcg cggtccgcgg
    30661 ccgacaggcc cagaccggat gccgtgacgt caccgaccac gagccgcacc cgtcccgcgg
    30721 tgcccgggtc ggtgacctcg agctccttga cccgggagcg ggcgagcgcg ccgtgctggc
    30781 gctgcaccag gcacaccgcg tcggtgccgg gccggcgggc cagcaggcgg gggaggagcg
    30841 ccgagccgag aaagcccggg aacccggtca tgagcagctg ccccacgtca ttcgcctctt
    30901 ccgaaggcgc tgccggctgc cccgggtgtt tcccggtcac gtcaccggct tcgccacctc
    30961 ccaccctacg gatcccgggc cgccggaggg gcagagggcg tccgagaaca cactcggcag
    31021 cccgagtcga cgcgtcaggt cagcgcgtcc gggccgatcg cgcccgccag ggcgcggacc
    31081 gcggcgtcgg cctgcgtctc gggcagcccc gcgtagcaga gcaccaggcc ccgcacgggg
    31141 gtcgccaccg cgtaggccga cagcgggctg accgcgacgc cgacctgctc gagccgggtg
    31201 acgacgtcga ggtcgtcgac gtcgtcgggc aggcgcagca cgacgtgcag gccggcctcg
    31261 accccgtcca gcaccacgaa cggaagatgg tcggatgccg ccgccaccag tgccgctcgc
    31321 cgcgcggcat aggtgcgggt cacccgggcg tggtgggtgg cgagcgaccc ggaggcgatg
    31381 aactcggcca gcgccagccc ggtggcctcg ttgacgacca gcccccggct ctccagctcg
    31441 acgcgcaacc gttcgcgcag ccgacgcggt gctgcgatcc atgcgacccg cagtgagggc
    31501 gcgatgagtt ttgacgccgt gccgatgtag acgacgtggt ccgcggcgcc cggcatcgac
    31561 cgcagcgcgg gaaggggtga gacgtcgtag cggaactccc cgtcgtagtc gtcctcgagc
    31621 acgagggagt cggtgcgccg tgcccagtcc agcagcagcg ctcgtcgggt gactggcatc
    31681 cgagcaccga gggggtactg gtgcgacggg gtcacgtaga cggcccgggc gctgcgcggc
    31741 aggtgggccg ggtcgaggcc gtcgccgtcg accgacaccg ccgcgacgcg ggcgccctcg
    31801 gcctcgaacg cggtccaggc ctcgacgtag cccgggtcct cgagggcgac ggtggacccg
    31861 acgaggccca gtgccacggg cagcgcccgc agcgacgccc cgacgccggg cacgacgagc
    31921 aggtcgtcgg gggccaccgc gacgccacgg ctgcgccgca ggtggtcggc gagcacccgg
    31981 cgcagtcctt cgtggctcgg ccccgcgccg gcgtcgttgc cgggcacggc ggtgcccgcg
    32041 gctcgccagg cgcgacgcca cgcggcggca tcgagcaacg tggtgtccgg gcgtccaggg
    32101 cgcaggtccc aacggggtgg ggcggtcgcg accgatcgga tgccgaccgc ctcgccgccc
    32161 gcggtgggca cgtggctgag cgccccggct gcggcggccc ggtcggcgcc cggggcgacc
    32221 accgcacccg agccggctcg actgagcacg aatccggcgg ccgccagctc gtcgtaggcc
    32281 gcgacgacgg ggccgcgcga gaggccgagg tcggctgcga ggactcgggt cgagggcagg
    32341 gggtcaccgg ggcggagccg cccggagcgc agttcgccga ccaccgcgtc agcgatccgg
    32401 tggcgcagcg gagcggcgcc cgcgggcacg gtgagcgcga gctcgaccgg tcggggggtg
    32461 cgcggcatcc ctgaaccata gtggtcctgt gatttcactg cctactggat ctcggcgtgg
    32521 gccactttgc cttgatgaga tggtgcgccg gtgcccctgt cggggtgccg agagaggatg
    32581 aggcagtgag ttggctccag atgcgacccg ggtgcgagtg ctgcgacatc gacctgcctg
    32641 cgggtgaggt cggtgccgtc gtgtgctcgt tcgagtgcac gttctgctcg gggtgtgctg
    32701 agcacctcga gcgcgcctgc ccgaactgcg gcggtgccct gatgccgcgc ccgacgaggg
    32761 tcggcgacgc cctgctgcgc cacccggcat ccacgacgcg cgtggtgtcc gccggcgagt
    32821 gccccggccg ggggtatgcc gggcgcggga gcgtggctgc cggtgcctcg cggcagcggt
    32881 tgcgccgtct caccgaccgg gcacgctcgg aacgcgccga cctcgacgcc ctcctcgacg
    32941 aggtgctcac cggcacgctc tccaccgtcg tcgacggtcg accctgggtc gtgcccatgc
    33001 tcttcgcccg cgacggtgac cgggtgctcc ttcacgggtc gacgggcgca ggcgccctgc
    33061 ggcacgtcgc ggccggggcc ccggcggtct tcagcgtcat ggtcgtcgac ggtctcgtcg
    33121 tcgcgcacac caccttcgac tcctcggcca actaccgctc tgcggtcatc cacggcgtgc
    33181 tgaccccgct cacgggtgag gaccgcgaga cctccctgga ccgcatctcc gaacggctca
    33241 tccccggccg gaccgccgag gttcggccga tgctgcccaa ggaggtcgcg gccaccctgg
    33301 cgatggagct gcccatcacc gacggccagt gggtgctcaa ggcccgctcg ggcggtgcgt
    33361 ccgtccctga cgaggagacc gacgcgtggt cgggcgtggt cccgttgcgc gtcgtcgccg
    33421 gtgaccccga acccgccccg tggtcggtgg aggccgcgac cccggtcccg gcatccgtct
    33481 cggccttcgt cgagcgggtg ggccgatgac cgcgccggtg cgggccaccg gcatcggctc
    33541 gtggcccggc gcgtcggtgc gtgaggccgt gcgcaccgtg cgcgacctgc tgctcaccgg
    33601 tgacggcgac gggctgccgc acctgcccga gaccccggcc cgcggtcccg gtgccgacat
    33661 catcgggcgg gccgccggcc tgctcgtcga cctgccggtc gacctgcaac ccagtggctg
    33721 gcgactggtc gaccgcccgg gtcgcgactc cgggcgcacc gcagccctgc accgcgagga
    33781 cctcgacgaa ctggccgagg cctacgacgg ctacgtcggc ccgctcaagg tccaggtcgc
    33841 cgggccgtgg accctcgtgg cgtcgctgcg gctcaaccgt ggcgagcggg tgatctcgga
    33901 tgccggggcg gtgggtgacg tgaccgcgtc gctgagcgac ggcatccaga actacgtcgc
    33961 cgacgtgcgt cgcctcgtgc ccggtgccga cgtcgtcctc caggtcgacg agccgtcctt
    34021 gcctgccgtg ctcgagggat cgctgcccac gtcatcgggg tacgggcggg tgcgggcggt
    34081 cgagccgcac gacgtcatgc gccgcctcga gcaggtgctt gccgcccacg acggtgagac
    34141 ggtcgtgcac tgctgccacc cctcggcacc gctgccgctg ctgcgcgcca ccggtgcagg
    34201 ggcgctggcc atcgacgtca cgtccgccac cccggcgcgc tgggagtcga tcgccgcgac
    34261 gctcgaggcc ggcaccgggc tgtatgccgg gtgcctgccg accgacggct cgggcaccga
    34321 ccagggtgca cgggcgctgg tcctcgaggg cttcgagcgg gccgggctgg cggccagttc
    34381 gctcacgggg ctcgtggtct caccggcgtg cggactggcc gggctcacac aacagggggc
    34441 ccgggccgtt gtgcgcacgg cgctcgacac ggcgcgccga ctcagcgagg aggttccggc
    34501 gtgacgaccc agccgcgtgt tccctggcag gtcaagtacc tggcgctcgt cctcatctgg
    34561 gggtcgagct tcctgctcat gaaggtcggt ctcgaggccc tgacggccgt gcagatctcc
    34621 gcgctgcgga tcctgtccgg ggcggtggtg ctgctcggcc tgctcttcgc gcgcgggggc
    34681 cggctgccga cgggggctcg cgtgtggggc cacctgctcg tgtgcgggtt cttcctcggg
    34741 gccctgccgt tcacgctctt cgccctctcc gagacgcgga tcagctcggc gctggcgggg
    34801 atcggcaacg cggcgacccc catcgcgacg gtgctcgcca ccatggcgat cctgccgggg
    34861 gagcgtgcca ccgggcgccg gctggtcgcc gtcggggtcg gcttcctcgg agtcatcacg
    34921 atcatgcagc cgtggacggc caccgaccgg cccgacctcg tcgggttctc gatggccctc
    34981 atcggtggcg cgagctacgg catcgggtgg acgtacaacc ggaagttcct cagtggggtc
    35041 gacctcggcg ggctctcgca gccggcggcc acgttgacga gcgcgctcgt gctcatggtg
    35101 ccgttcgtcg tcacgtgggc gctgctgcag cccgaaggcc tggcggcgat ctgggcctac
    35161 gacgccgcgg gggtcggcga cctgccctgg ctaccgctgc tgtgcgtgct cgtgctcggc
    35221 gtcgtcggca ccgggttcgc ctacatgctc cagttcgacg tcgtccgcgg ggcggggccg
    35281 gtcatcggct ccacgatcac ctacctcatc cccgtggtgt cggtgctgct cggtgtgctc
    35341 gtgctcgggg agcacctcgg gccctggcag atcgtcggct tcgtgatcgt gctcggcgca
    35401 gcgttcgtca tcaaccagaa gcccgcagcc cgacccgcaa cggacgccac ggcatccacg
    35461 gtgggcgagg tgacgacgac ggccaggtga ggtcgacgtc atctggtggg gcagattcga
    35521 ccacgtggtc agatccgccc caccacgcca ggctcactca gccggggcgt gcacctcgcg
    35581 gcgcaggatc ttgccggtcg ggcccttggg cagctcaggg acgacccaca cggtgcgcgg
    35641 gtacttgtac gccgcgatcc gctccttggc gaagtcgatg atgtcctgcg gggtcgccgt
    35701 cgaaccctcg cgcagggcca ccgctgcacc gacctgctcg ccgtagagct cgtccgggat
    35761 gccgacgaca gccacctcga gcacgtcggg gtgctcgtag atgcactcct cgacctcgcg
    35821 cgggtacacg ttcatcccgc cgcgcaggat catgtccttc ttgcggtcga cgatggtgaa
    35881 gtagccctcg gagtccatgg tcgcgaggtc accggtgcgg aaccagccgt cggggatggc
    35941 ctccgcggtg gcctcgggct tgttccagta gcccttcatg acgttgtcgc cgcggatggc
    36001 gatctcgccg acctcgccga cgggcacctc gttgccgtcg aggtcgacgc acttcatctc
    36061 gcagccgggg atggccacgc cgatcgtgcc gggcttgcgc tccatcgtcg gcatgttgaa
    36121 cgacgcgacg ggcgaggtct cggacaggcc gtagccctcg aggatgacgc agccgaactt
    36181 ctcctcgaac gagcgcatca cctccagcgg catcgcggag ccgccggacg cgcaggtgcg
    36241 cagcgacgac gcgtccatgc cgtcggatgc cgggtggttg aggatcgcgg cgtacatcgt
    36301 cggtacgccc tgcatgatcg tcacccgctc cttctcgatg acctcgatcg ctgcggcggg
    36361 gtcgaagcgc gggatgaggg cgagcgatgc cccggcgatg gtggcggcgt tgagcccgac
    36421 gaccaggccg aagacgtgga acagcggcag gcagcccatc acgatgtcgt cggccgtcat
    36481 ctgctggatg accttggccg agcgcagcgc gttgaggtgg atgttggagt gcgtcagctc
    36541 ggcacccttg ggccggccgg tcgtgccgga ggtgtagagg atgatcgcgg tgtcgtcgtc
    36601 ggcgcgctcg tgcgggtcgg tgatcgggtc gccgccctcg aaggagccgg ccaccgggcc
    36661 catcgggccg cactcgatga tctgcgtgcc gctgttctgg gcgcccttgg tggcctcggg
    36721 cacgaagtcg ggccagacga aggcgatctt cgcgccggag tcggtgaaga agtagtcgat
    36781 ctcgccggcc ttgagcagcg ggttcatcgg cacgacgatg ccgccggcca gcaggatgcc
    36841 ccagtagacg accgggaagg ccgggacgtt ggggaggatg atcgcgaccc ggtcgccggg
    36901 ctggatgccg ttggcgcgca gcgctccggc cacggccgcc gccatggcgt gcaactgggc
    36961 gtaggtcacg ccctggccgt tgatccgcag cgcatcctgc tcggcgttgg tcgctgcggt
    37021 gctcgtgagg ttggtcgcga ggttggtcat cggggctcct gggctcggcg gctctgtcgg
    37081 gctggcccat cgtcgcacgc gtttaggctg gggcgatggc gagctggcag atgcgtgcgg
    37141 tggcgctcta cgttcgcgcc acccgcaaga agcgttacgc cacccgcgag gccggcctgg
    37201 cgttcctcca ctccgccaag ggcccctccg acccgcccgc gaaggcactc ggtgacagcc
    37261 gcgtgagcac ccaccgggtg cggggccgcg acgtccacgt ggtgcgcgga cccggccaca
    37321 cggcatccgc tgcaggggcg gtcgtctacc tccacggggg tgcgtacacc aacgaggtcg
    37381 tcgaccagca gtgggcgttc gtggctgcgc tggcggcgcg cacgggcgtc gaggtgcacg
    37441 tgccgatcta cgggctcgcg ccgcaccaca cggcgtccga ggcggtcgac ctcgtcgccg
    37501 aggtcgtgca cggtctcgtc gcccaggggc gttcggtggt gctgctcggc gactccgcgg
    37561 gcggtgggct cgccctggtc gccgcccagc aggcggcgcc cgccgtgcgc cagcgggtgg
    37621 ccggcctgct gctgatcgcc ccgtggatcg acctcacgat ggccaacccc gccgtggatg
    37681 ccgtcgagcc cacggacccg tggctcaccc gggcgggcct gcacccgatc gcgcggtcgt
    37741 gggcgggtga cctcgctctt gacgaccccg tcgtgagccc cctcttcggc gacctcaccg
    37801 gcctgccgcc ggtcgaggtc tgggtcggga cccgcgacat caccgcgccc gactgccgcg
    37861 agctggagcg tcggatgccg gacggtgccg ccttcgcgct ccacgtcgag gacggtgcga
    37921 tccacgacta cccgctgctg ccgactccgg agggtcgcgc cgcgagtcgc gagatcggcg
    37981 agcgcgtcac cgcgctgctc acggcatccg gctgaccctc ggcggatcgc tcagatgacg
    38041 ccgagcagca ccatggcgtc ggcgaccttg gtgtaggccg cgaggttggc gcccatgacg
    38101 tagttgcccg ggtcgccgta ctgctcggcc gtctcgacgc actgcgcgtg gatggaggtc
    38161 atgatcgcct cgagccgttc ctcggtggcc tcgaaggtcc acgagtcgcg gctggcgttc
    38221 tgctgcatct cgagcgcgct cgtggccacg ccgccggcgt tcgacgcctt gcccggggcg
    38281 taggcgacac cggcttcctg gagcagcgcg accgcctccg gggtggtggg catgttcgcg
    38341 ccctcggcga cgagctcgac gccgttggcg atgagcgcgc gggcgccgct ctccgggagc
    38401 tcgttctggg tggcgcaggg cagtgccacc tggcagggca cgtcccagat ggacccgccg
    38461 acgacgtgcc gggcggagcc cccgcgggcc tcggcgtagt ccgacagccg gccccgccga
    38521 acctccttga cctccttgag caggtcgagg tcgataccgg tctcgtcgac gacgtagccg
    38581 ccgctgtccg agcaggcgac gacggtgccg ccgagctgcg cgaccttctc gatcgcgtag
    38641 atggccacgt tgccggagcc ggagacgacg acgcgcttgc cgtcgaagga ctggccggcc
    38701 gtcgcaagca tctcccgggc gaagtacacc gtgccgtacc ccgtggcctc ggtgcgcacc
    38761 tgcgagccgc ccacggagat gcccttgccg gtgagcaccc cggactcgta gcggttggtg
    38821 atgcgcttgt actgcccgaa caggtagccg atctcacgcc cgccgacgcc gatgtcaccc
    38881 gccggcacgt cggtgtactc gccgatgtgc cggtagagct cggtcatgaa cgactggcag
    38941 aagcgcatga tctcggtgtc ggagcggccc ttggggtcga agtcgga
//
LOCUS       DAOSOC010000005        38508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1098_length_38508_cov_9.712142, whole genome shotgun sequence.
ACCESSION   DAOSOC010000005 DAOSOC010000000
VERSION     DAOSOC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 38508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38508
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1098_length_38508_cov_9.712142"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="PL014_00850"
     CDS             complement(<1..1532)
                     /locus_tag="PL014_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT55667.1"
                     /translation="MSNTSPELGALPPEFSHEALQTLIRIGAATGSVTGEQVAHAMQS
                     AEVKPKRGRVVLSALGEHGIEVVMDPAHIGVAAATARTTKKAAASASTAKKAAATKAA
                     PAKAAATKKAPAKAAATKAAPAKAAATKVAPAKAAATKAAPTKAAATKAAPAKAAATK
                     ATATKATATKATATKATATKAAATKTAAAKTTASSTAAAKTAAPRAARATTTRKAAAA
                     AAGSDAPAKTTVPRRSAAAKKAAAAETEAAAEAVDELEDDEDDDEVKKPAEPETETSG
                     FVLRDDDEDDAPVQQVVTAGATADPVKDYLKQIGKVALLNAEQEVELAKRIEAGLFAE
                     EKLNSGEKLDMKLKRELWWIAQDGKKAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQ
                     EGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLA
                     RVQRQMLQDLGREPTPEELAKELDMTPEKVVEVQKYGREPISLHTPLGEDGDSEFGDL
                     IE"
     gene            complement(1781..2176)
                     /locus_tag="PL014_00855"
     CDS             complement(1781..2176)
                     /locus_tag="PL014_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT55668.1"
                     /translation="MTIVVGFVPTKEGRAALSRAVEEARLRQSKLVVINSNRGGRDYD
                     DDTSQTAEAELQRVTDELRGDGLELEVRQLVRGNEPAEDLIAVANDTDADLIVVGLRR
                     RTPVGKLILGSNAQRILLDAPCAVLAVKA"
     gene            complement(2242..3372)
                     /locus_tag="PL014_00860"
     CDS             complement(2242..3372)
                     /locus_tag="PL014_00860"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOT55669.1"
                     /translation="MTATPTRSTSAVWRAGDPVGDRLFAAVGDLSLERGGVLPDVTVA
                     YESWGTLSPSGDNAVLVEHALTGDSHVVGEVGPGHPSPGWWPGLIGPGAPLDTNELFV
                     VASNVLGGCQGTTGPASAGPDGRPWGGRFPFITIRDQVAAEVALADALGIERWHGVLG
                     GSMGGMRALEWATGHGARVERTLVLASTPYATADQVAWCQPQLLAIRSDPAYRGGDYY
                     GYEDWPVTGMGIARRIAHTTYRHEGELDERFGRSPRAGEDPLGGGGRYDVESYLDHHA
                     AKLTRRFDPNSYVVLTEAMNSHDIGRGRGGVASALQAYRGALHVAGVSTDRLYPLRLS
                     EQMVADRTGSELSVIESRYGHDGFLIETDQVGAVIARALGAA"
     regulatory      complement(3389..3499)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            complement(3555..4697)
                     /locus_tag="PL014_00865"
     CDS             complement(3555..4697)
                     /locus_tag="PL014_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HOT55670.1"
                     /translation="MTPDITLRGPGDIVSVLPYHLGYHPRDSVVVVSIRGRLLGLVAR
                     VDIPPPGREAEVATWLTGPLVRDGASAAVVVSFEDRADESVPISLAVAEHLEQAGVEV
                     LDVMVVRDGRRYSPRCAQDCCPAEGVALPDPADVVGVAEYVARGRAPLLSRRAVDALV
                     QEDPVVSTGVAAAIGAREGMPRRRLRRRSARAWAELLRPPRTEGGARDGGGRDGLSRD
                     GGAQHPPPTVVADLALGLRDIAWRDAVIAWLAPGVLPLADLQPSAVLMLESTMPSWGG
                     MGFDGEPGEGSDEVLRRLVALCRSLPDEAAQEAAALCTVVAHVAWASGDGTIARAAIE
                     RARHLAPDYRLAALLAKLIDAGIRFPPVPSTEGVTQDGDGRLGRAG"
     gene            complement(4861..5280)
                     /locus_tag="PL014_00870"
     CDS             complement(4861..5280)
                     /locus_tag="PL014_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOT55671.1"
                     /translation="MASPKRFAAIAAVYQAVRVAVRPGGPSLGERAGAFPRLVRAAIT
                     GEYTGISKGRLALMVAALAYIVSPLDLIPEFVMLAGLADDALVMAWLATQFVEETEAF
                     LAWELAQVSPAYAATSGHAFMGGTPSSSHTVRGDVIR"
     gene            complement(5441..6178)
                     /locus_tag="PL014_00875"
     CDS             complement(5441..6178)
                     /locus_tag="PL014_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HOT55672.1"
                     /translation="MALPGKGGDRTSDAVKDVRSALELAQDGTADQGVLDRQSTKFVQ
                     NILDLGVDGKGWFDSAAHEADAALRENGGDVEAAVDAIVRSHVTKGAGTGFVTSVGGF
                     VTLPLALPVNLLAFYLLATRMTASIAKVRGYDLSSEQIRTAVLLTLVGADADDLLRKA
                     NVVVPGGTLSNLAVQRLPGPVLMVVQKGIGFRLLAQVGTKSFARLGRLVPVVGGVVGG
                     GLDAYMLSRFARNAKDQFPRVAPAIER"
     gene            6300..8000
                     /locus_tag="PL014_00880"
     CDS             6300..8000
                     /locus_tag="PL014_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOT55673.1"
                     /translation="MIPGAVAALAVTPRGAGRRYAALVHDGGCDVIALEQAADAVRSL
                     DARLSPRWVWWAASDAAAPLVEAGIPLARAWDVAEAHRLLHGGWSATAGVCWAAAHGI
                     PTDTAPAPPTGDLFEFASEAAPLAADALVDGAGHLRGDHESWLRDPAHLEAWARAALE
                     TAHRQHDAAAATSVRLPSTVYSESAAALLCLELQRDGLPIDRETTEALIAGAAGPRPT
                     TDADEAASRRVRDAQVLRLAPGRESTDLRNPAQVRELLAAVGVDVPNTRKWVLEPYRS
                     VNPLVEALLTWRRDERIATTYGYRWLDAHVGADDRLRGRWTACDGAAGRMTAENGLHN
                     LPAALRPGVAAHPGHVFVRADLGQIEPRVLAVVSGDAVFAAATQADDLYAPVAQRLGV
                     ERPVAKIAVLAAMYGQRSGAAGEALKGLERAYPVAMGLLDRAYAAGVRGAPLRTYGGR
                     LIPTGSVLTGAGVGADPALDAARGRFARNAIIQGAAAELFKAWGATVRATTRELGAQV
                     VLCLHDELLVHAPLEHAGEVSARVDRALQDASRRWAGGAPVRFVADTSVIHRWSEAKD
                     "
     gene            complement(8107..9024)
                     /locus_tag="PL014_00885"
     CDS             complement(8107..9024)
                     /locus_tag="PL014_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOT55674.1"
                     /translation="MLNPSALYRFETDADLTVPRPGVLVVTLGAFVDAGQIQSVLGGH
                     LLETGDPEVVASFDVDQLLDYRGRRPAMVFDTNRWVEYDDPTMLLHRLTDRDGQTYYL
                     LTGPEPDYQWERVIEAVRELVVRLGITLVVHTHGIPMAVPHTRPVGMTAHATDTSLIG
                     DSISPFGRVQVPGSIGALLEMRLGESGHQAVGFAIHVPHYLAGAEWAEGALAALNAVV
                     DVTGLNLPNDDLVLRARSNQQAIAAEVEGNDDARAVVSALEQQYDTYLEGKERPSLLA
                     TEASQLPSADELGADFENFLRHVADDDTA"
     gene            complement(9103..>11544)
                     /gene="hrpA"
                     /locus_tag="PL014_00890"
     CDS             complement(9103..>11544)
                     /gene="hrpA"
                     /locus_tag="PL014_00890"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOT55675.1"
                     /translation="PLDPRLARMVIEAGKFGCTREVLVIVSALSIQDPRERPVDKQAQ
                     ADQSHARFKSERSDFEALHTLWRYLKDQQKALSHSAFRRTCKAEYLHYLRIREWQDLH
                     SQLRAACRSVRIDPDVATSAPDAEPDWDTIHKALLAGLLSHVGVRDQAKREYVGARGA
                     RFGISPGSGLFRKQPDVVMSAELVETSRLWARVNAAIEPEWAEEAGAHVVTRTVSEPR
                     WSRRAGAAVATERVTLFGVPLVTDRTIQYARIDPEAAREIFIRSALVEGDWDPQHDFW
                     KRNLATLARVAELEERARRRDIVVDDEVLNAFYDSRIPPDVVSERHFDRWWRGERRRR
                     PDLLTLTEEILTADSGEQVSAADYPRTWRQGDVELDVTYQFSPGADADGVTVHVPVAV
                     LNRVTSDGFDWQVPGLRDDLAVALLKSLPKATRRHFVPTPDHAAAALAEADPSRGPLT
                     AELARVLHARTGIRISPDEWDVERVPDHLRITFSIEGPGRRVVARGKDLDALREQAGG
                     AVRAGMARAGASLERTGLTTWSVGDVPATFEGRSGGLTVQGFPALVDEGTSVALRVLP
                     DAGAARAAHRAGVRRLLLLGTTAPWKRVLARLSNAQKLALADNPHGSVPALLQDCLDA
                     AVDDIVAEHVPGEVRTQAAYEAALSAVRTHAATRVLVVVEAVEPVLALAAQVRATLAT
                     LERGAASARTAATRADVRAQLDSLVRPGFVAATGVARLRDVQRYLRAAQHRLERAATN
                     AREGVLQAQIDGVEAAYADLLESLPPVRRGAADVVDIGWQIEELRVSLFAQALGTPRP
                     VSEKRVRAAIAAIDR"
     assembly_gap    11545..11644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11645..12966)
                     /gene="hrpA"
                     /locus_tag="PL014_00895"
     CDS             complement(<11645..12966)
                     /gene="hrpA"
                     /locus_tag="PL014_00895"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOT55676.1"
                     /translation="MPAQHVDVSAPGAVDPAELRYPEHLPVVERRDDILAGIRDHQVV
                     VIAGETGSGKTTQLPKMCLELGLGATGQIGHTQPRRIAARSVAERIAEEMGVELGDLI
                     GYQVRFTDHSSDATRVKVMTDGILLAEMQRDRDLRRYDTIIIDEAHERSLNIDFILGY
                     LKQLLPRRPELRVIVTSATIDPVRFAVHFATDEHGREVREVPVIEVSGRTHPVEVRYR
                     PLVELHPEGRHVVAERDQVTGVVEAVEELWTETPPSPEATDILAFFSGEREIRDAADA
                     LNALKLPNTEVLPLFARLSAAEQHRVFHRGSGRRIILATNVAETSLTVPGIGYVVDTG
                     TARISRYSQRTKVQRLPIEPVSQASAAQRTGRCGRIAPGVCIRLYSEEDFEARPEFTE
                     PEIQRTSLASVILQMTALGLGDVARFPFVDPPDPRQIADGVRLLEELGA"
     gene            13045..>13804
                     /locus_tag="PL014_00900"
     CDS             13045..>13804
                     /locus_tag="PL014_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOT55677.1"
                     /translation="MTASENPATDGGSLVDRTNYEGATFERANFKGATIRFSDVSGMT
                     MRSVDVGGLDIDSHDLAFGFLFVNGVDVVPLVEAELNRQFPGRELQKATTPHGLREGW
                     VAVQAAWQHTVGDTPAPLVDAHVPDEWSLAQTLRHLVLATDAWLRSAILQVPAPFHEI
                     GQLFTGAEAMGVDVSAFRDDVPFDEVLRVRAERQQMVTAFLDEVTQETLAEPRNDPWG
                     DEDWHPSVGDCVRVILEEEWAHLRYIRRDLALLTQ"
     assembly_gap    13805..13904
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14000..15202)
                     /locus_tag="PL014_00905"
     CDS             complement(14000..15202)
                     /locus_tag="PL014_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55678.1"
                     /translation="MILEPLSGCPSGVRDLADRLTASSRRVGDVTTVLVALKGGATWV
                     SSAGEAFGARLAEAPPLLDAVARRLGGAAVPLREIADAMEEAQRVVEGAIRDDSEAQG
                     TYALLEDRAYALISAGADETSPDVVAVRILQRDQVRIRERARARHAAAMERFRAVDAR
                     CSASVRALTQDAVTDSALYRLVAGSQTVGRDLAAVGTVATVVPYLRPVGAAGDVLSVA
                     ADTTLLVGYGEGAWSELGTTVAIAAAGNVAGALKSAGTAGATKSASGSVFTRTRRLTT
                     QQRLAVGVTVQARAKRDAIRARFRVQADDRATPTRFIGVTPRPVRTTSVWKLSREQVV
                     ARVRTTAQAKADQVFLDGWRRASANGPAAQKMYAAGTTLDLGVKVAPKVLPTVVPQQE
                     PHRAQSAR"
     gene            complement(15199..15486)
                     /locus_tag="PL014_00910"
     CDS             complement(15199..15486)
                     /locus_tag="PL014_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55679.1"
                     /translation="MTIIRVDGAQVAELGVSLTEVGADFLLMQDPSLERMVLGPGECG
                     PAVEELLGGWRRQRIALGRALGSLGEAAVSAGTVYVDTEAGVTRLFGGAQS"
     gene            complement(15570..16913)
                     /locus_tag="PL014_00915"
     CDS             complement(15570..16913)
                     /locus_tag="PL014_00915"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HOT55680.1"
                     /translation="MTDPSQWGFETTQIHAGQAPDPTTGARALPIYQTTSYVFNDTTH
                     AANLFALKEFGNIYTRIMNPTQDAVEQRIAALEGGVGALLVSSGQAATTLGLLNIAEA
                     GDHIVASSALYGGTVNLLKYTFKKLGIDVTFVEDQADLQAWRDAVRPNTKAFFAETIA
                     NPKAEVLDIAGVAGVAHEAGVPLVVDNTIATPYLIRPLEWGADVVIHSATKYLGGHGT
                     SIAGVIVDGGTFDYGQDPERFPNYNTPEESYHGLVFARDLGVGGALGANLAYILKARV
                     QLLRDLGSAISPFNAFLIAQGIETLSLRVERHIENARAVAAHLAAHPQVESVRWASLD
                     GSPSKALADKYAPKGAGAVLAFEIAGGLEAGQRFVEGLTLHSHVANIGDVRSLAIHPA
                     STTHSQGGDEDRLAAGVTPGLVRLAVGLESIADILADLDAGFAAAAGGASATAAE"
     regulatory      complement(16929..17043)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            17128..>17760
                     /locus_tag="PL014_00920"
     CDS             17128..>17760
                     /locus_tag="PL014_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT55681.1"
                     /translation="MTLQADEYQAAPGRYEAGMPYRRVGRSGLDLPAISLGLWHNFGD
                     DVPLERQQATLRRAFDRGVTHVDLANNYGPPYGSAERNFGTIFARDFSRYRDELIISS
                     KAGYDMWPGPYGQGGGSRKYVIASCEQSLKRMGLDYVDIFYSHRFDETTPLEETMGAL
                     DSLVRSGKALYVGISSYSGERTREAAAILREMGTPLLIHQPSYSMLNRWIE"
     assembly_gap    17761..17860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17861..18306
                     /locus_tag="PL014_00925"
     CDS             <17861..18306
                     /locus_tag="PL014_00925"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HOT55682.1"
                     /translation="TETTDTYIPSPSDWVREQVETIEATGDTRSVDIMGLPVVLLTMR
                     GKKTGALRKVPLMRVEHDGVYAAVASKGGAPEHPQWYANLLANPTLTLMDGTASWDAV
                     AREITGEERELWWERCVQAFPNYAEYQTNTDRTIPVLLLEPVSAG"
     gene            complement(18309..18761)
                     /locus_tag="PL014_00930"
     CDS             complement(18309..18761)
                     /locus_tag="PL014_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55683.1"
                     /translation="MPERDPGQPRGDAPGQPRGYRRALGGPGVILVTVYGVLALAATG
                     RSILQITTDFGAAPLAYTLSALAAAVYVLATWCLARGGRWVRVGVIACAVELVGVLVV
                     GTASFLVPDAFPDKTVWSHFGQGYGFVPLVLPVLGLWWFRRVDRAVPA"
     gene            complement(18754..19116)
                     /locus_tag="PL014_00935"
     CDS             complement(18754..19116)
                     /locus_tag="PL014_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55684.1"
                     /translation="MIAPLTYAVVGLAAVLLVVSGFYAVRDRLIDDRVLLLAALLEIG
                     LIVQLVLGLVGLGEIEDSTDRATFVAYLVSLPVIPISTVLLTIKEKTVWAMGSLAVGA
                     FAVAVMTVRLQQIWNLNA"
     gene            19150..20052
                     /locus_tag="PL014_00940"
     CDS             19150..20052
                     /locus_tag="PL014_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT55685.1"
                     /translation="MTSAQLGDVTIEFEIDGPDDGIPMLLVMGLGAQLVAWPTPIIDG
                     LVENGFRVIRMDNRDIGLSTKTQGPAPTRADLLKAFAHRRYAQSDYLLADMAGDAVAL
                     LDHLDIEAAHVVGVSMGGMIAQQLTIDHPTRVLSLCSIMSNTGSRLHGTVAPKLLPTM
                     AMTMTTPRSTDPDEAERLGVESFRLIAGPHYDADEMAVMVRRAIDRDINPLGTARQLL
                     AIQASPDRTPGLRTITVPTVVIHGLLDQLVLPSGGIATARAIPGSRLVMFPDMAHDLP
                     GPRRAEIVDEIVRNAARAKVSTSS"
     gene            complement(20102..21352)
                     /gene="rocD"
                     /locus_tag="PL014_00945"
     CDS             complement(20102..21352)
                     /gene="rocD"
                     /locus_tag="PL014_00945"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOT55686.1"
                     /translation="MTSDSSTSHVGSAQTAAHIAQVERYAAHNYHPLPVVLSHGEGAW
                     VTDVDGRRYLDCLAGYSALNFGHGHPRLLARAHDQLSRLTLTSRAFHNDQLGPFSQAL
                     AELTGKDMVLPMNTGAEAVETALKVSRRWGYRVKGVPEDQANIIVMEGNFHGRTTTII
                     SFSDDKVARDEYGPYTPGFRTVTYGDVESLRQAVDEFTVAVLLEPIQGEGGVVIPPEG
                     YLRAVRQICDEAGVLMLADEIQSGLARTGRTFACDHEDVVPDIYVMGKALGGGLYPVS
                     AIATNREILEVITPGSHGSTFGGNPLAAAIGHEVVTMLTEGEFQARATELGGLLTELL
                     TPLVRTGHGNGLSGIRVRGLWAGLDIEPGGPSGREVSERLMRRGVLAKDTHGSTIRIA
                     PPLVITADEVGMLVDAVADALERP"
     gene            complement(21461..21844)
                     /locus_tag="PL014_00950"
     CDS             complement(21461..21844)
                     /locus_tag="PL014_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55687.1"
                     /translation="MAALDVVPLMKFPASWPVPVVATVAMAVLAGLDLLGAIAAKEWA
                     ERQSIAAIAFGTIASVALFWFYASSLQYAELAVVTMGWIVLLQVGIIIVDRVHFGTVL
                     GPDKIGALIIILVAQGYLVLAPSST"
     assembly_gap    22032..22131
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22161..23168)
                     /locus_tag="PL014_00955"
     CDS             complement(22161..23168)
                     /locus_tag="PL014_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT55688.1"
                     /translation="MSNIAPQVQQLALEQTPGGGIGPASGVSGDGDAPRGSVAVSATT
                     DGGDEFAGWQPPTTDNERAFVLAGGGATGIAWEAGVIMGLRDGGVDVREADTMIGTSA
                     GSMVAAHLRVGTQESLAFARIREGAQIAEIGRLGPADASRYLRAQMSLDKKRGRALVG
                     QAALAARTTTEEEWLKAIGLGLVGKEWPDKRLFITAVDARTGTSVVFDNSSGVPLERA
                     VAASCAVPGVFPAVEVGGRRYVDGGLRTVANADLAVGHRRVLALSPYPVASHLRDLPA
                     RQLRSLRPASRTHLVVPDARDLWAMGANPLDFSRGADTFETARAHGQRIADRVGEMWL
                     A"
     gene            complement(23352..24053)
                     /locus_tag="PL014_00960"
     CDS             complement(23352..24053)
                     /locus_tag="PL014_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HOT55689.1"
                     /translation="MEITRALATGRRLLREHGLDDWTIVADRAKTRAGVCRYSRREIG
                     ISGPLTTLHDESEVLDTILHEIAHALVGPRHGHDAVWQAKAREIGSSGRQYVSPEAPR
                     VPKDWVGRCPAGHEKSRHRGPTRLMSCGQCSTRFDPQHLFTWTYRGRRVPLPPSYAEQ
                     LAALAETVRPTATRRPPVHLGDVVHVLSGPWGGRSGDVEHVGTLRCQVRVGDELVSLP
                     LELVRLEPATAARSV"
     gene            24302..26098
                     /locus_tag="PL014_00965"
     CDS             24302..26098
                     /locus_tag="PL014_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOT55690.1"
                     /translation="MPTPTLPIGLRLLRNYSGRHLPQDVLAGLLVAALAVPQALGYAV
                     VAGVPVQVGLYTLPPALLAYALFGSSRLLFVGPVSTVSVLSGSIVGALSDGDRALAIT
                     LTATLALTAGAVLVAVGLLRLGWVAQFLSEPIVTGFVAGLVVLIVIGEIPALAGVSAP
                     SGGIFQRIDETVRNLGDFHGVTLAVGASALGVLFVGSRLLPRAPWSLIVLAGGIAVST
                     TADLASRGVRVVGEVPTGLPLPSMPVVDVQLWSGIITGGIAIAAVGIAEGLAAVRTFA
                     PTGAIDPESDNREFVGHGAANLASGIFGGMGVGGSLSKTAANARAGAFTQVSGVAAAV
                     IVLLFVAFAAALLSDLPRAVLSAIVIHAVWGLVSPSTFRRYAAVRRNDFVAAVVAFGG
                     VLFLGPLNGLLLAIAQSLLGLVYRSMQVGVDEMGRVEGEKAAWGSVGNDSSRRTYRGV
                     TVLRPDGPLFWANATSVVGHIEMATRARQPLYAVVLDLEATNQMDTTTAERLDGLLTR
                     LRAEGTDLYLVRVFGNVRDVLSKSGFLDTLGPDHVWHSIAAGVKAARAARAAGIDPAN
                     PFPTPDEVDPLDEPDELDEGAGEQIASKGERP"
     gene            complement(26165..28111)
                     /locus_tag="PL014_00970"
     CDS             complement(26165..28111)
                     /locus_tag="PL014_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl cyclase"
                     /protein_id="HOT55691.1"
                     /translation="MAPDGARRRTRATASVVAVGALTLAAAVSTATAAAATSAAPAAS
                     TASVASLSSVVAALPAAATQTPFGDNVIVFDPSMPVSDIEKVVNDIHAEQVDDEMGTN
                     RWSLFFKPGTYGTAEDPLQIRVGYTTEVAGLGADPGDVVINGKVESFNRCIRDGGTSY
                     CVALNNFWRTLSNLTINVNGAGQDGCRQTANFWAVSQAVSLRRVEVTGGNLSLMDYCS
                     AGPQFASGGFIADSKAAFVINGSQQQWLTRNSEVGGWSNGVWNQVFAGVEGAPSDANF
                     PAEPYTTLETTPLSREKPWLWMDDSGAWKVRVPAAAAETRGTTWGDGLTAGRTLDLSE
                     FHIAKPGDSVQSINSQLARGKNLLLTPGVYDINRSIDIKRPGTVVLGMGHATLTAVNG
                     ATPITVWDRAGIVVAGVTIDAGLKNSKVLMQVGADRPRNPAQGSTLANPVTLSDVYFR
                     VGGPHIGKADVSLQIDADNVLVDHTWVWRADHGVEGFTGPNEGDSQRWATNTGRNGLI
                     VNGDDVTATGLFVEHYQEHNTIWNGERGRVVLYQNELPYDPPSQADWTQADGTLGYPG
                     YKVGATVKSHQLWGGGSYVFNRNDPSIVTENGFEVPNTPGVKMTHLLTVNLDAGTIRH
                     VVNGVGDQVDATRSGQPAYVGSYP"
     gene            complement(28397..28741)
                     /locus_tag="PL014_00975"
     CDS             complement(28397..28741)
                     /locus_tag="PL014_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018763058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT55692.1"
                     /translation="MPVAHEVFPAACPSRVVLDHVTSKWGILVLVALADGPQRWSELR
                     RRAEGISEKMLAQTLRTLAADGFVHRDAQPVIPPRVDYSLTPRGDELVAHLLPLFGWV
                     VAHADEIVAQRG"
     gene            28911..29765
                     /locus_tag="PL014_00980"
     CDS             28911..29765
                     /locus_tag="PL014_00980"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT55693.1"
                     /translation="MTIVVTGATGQLGRLVVESLLRRGASPTDVVATGRDEARLAELA
                     TSGVRTAVTDYGDAASLAAAFQGADRVLLISGSEVGQRLPQHTSVVEAAKAAGVGQLL
                     YTSISHADTAQMALATEHRETERVIRESGIPFTFLRNSWYLENYLSQVPTYLEHGVVG
                     ATRGGRISAATRADYADAAAVALLADDSAGQVLELGGSSFTLDELAATVASVSGREVT
                     ATEVSAEELTAILVGAGLPQGYAQVLASADEGISRGELEVSTGDLERLIGRPPTTLRE
                     AIEQTLAP"
     gene            complement(29874..30884)
                     /locus_tag="PL014_00985"
     CDS             complement(29874..30884)
                     /locus_tag="PL014_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HOT55694.1"
                     /translation="MKFRHLGNSGLKISEITYGNWLTHGSQVENDIATQCVRAALDHG
                     ISTFDTADVYANTKAESVLGEALKGERRESLEIFTKVYWPTGPGGKNDTGLSRKHIME
                     SINGSLTRLQTDYVDLYQAHRYDTETPLEETMTAFADVVRQGKALYIGVSEWTAEQIR
                     AGHALAKELNVQLISSQPQYSMLWRVIEGEVVPTSEELGISQIVWSPIAQGVLTGKYV
                     PGQAPPQGSRAADEKGGADMIKRFMNDDVLTRVQALKPVADEAGLTMAQLAVAWVLQN
                     ENVAAALVGASRPEQVAENVKAAGVKLEPELLARIDDVLGEVVERDPALTAANAPKGR
                     VA"
     gene            complement(30884..31141)
                     /locus_tag="PL014_00990"
     CDS             complement(30884..31141)
                     /locus_tag="PL014_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOT55695.1"
                     /translation="MLFIGIILFGMLVGAAAQLILGRGGSGINWPLAFGTGIAGSFVG
                     GLLASLLAGDGFNLRPSGIIGSIIGAIIVTLGYDRLKGSKA"
     gene            complement(31151..32293)
                     /locus_tag="PL014_00995"
     CDS             complement(31151..32293)
                     /locus_tag="PL014_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOT55696.1"
                     /translation="MITPDSAASNHMAPNPAASTPEPRRVLVTGATGYIGSRLIPRLL
                     RDGHHVRAAVTNLERARTAWWADDVELVRMDVLDSATVEAAVEGMDAVYYLIHALKGE
                     DFTTTDRISAENMAWAVAAAGVELLVYLSGLVPDVPENELSEHITSRLEVERILTGAT
                     AGIRVLTLRAAIVTGSGSTSFEVVRQISERMPVHTVPAWMDSRVQPIAVVDVVEALSG
                     ALAVESESRSYDVGGPERMPYTQLLDRYARIAGLTRPQVTVPGLPTDVVAVLAGAITD
                     VPGSTVPALVESLHHDMVCHDVDWTYDLLPEGYELVGVDESFARALREPDLTLPPEER
                     DPLGPMPGDPAWAGGSGTTTGGVGAVMAGARSVVSGLAGRMLPGRG"
     gene            complement(32372..34156)
                     /locus_tag="PL014_01000"
     CDS             complement(32372..34156)
                     /locus_tag="PL014_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT55697.1"
                     /translation="MSSTSVALAVAVLGLVWAVSGLAMALLSRATVSAVALTVAGAGT
                     LAATITQAMTTQAMTTQTTGAPGLTRFLLVASWALAMPLAVTAYPALQWRHPVDAVAL
                     TTVVGAGILATLQPQSQPVLDATGVVLGSVLVVHTWWRLERAPRRARSALAWLAVVGT
                     GAALIGLVVGFLTPTPAGAVVATLAAALVGPAMLVGATRPDVVDVRVVVVQLVVVTAV
                     LIGYIALFTLLASLLEVILGRPAAVAELAVAGALATLPLPALRTALRGVVDELLFGLR
                     ADPLDAATHVAGSDVGDPVLALRAVREALDLPYAVLRVAGESVASSGRSATSTTTLPL
                     TLGADVVGELEVGLRPGEVSLSGDDRNVLALVAPLLALTARSAALAAALQESREQSVT
                     AIEEERRRLRRDLHDGLGPRLSGIAFTADAARNSVRTDPDAAEALLAQLRAETSTAID
                     EIRGLVYAMRPPALDELGLVRAIRQQALALRTPEGTPLRLTVQAPPTLPPLTAAVEVA
                     AYRIVVEALTNAARHSGSDTVTAALSADAGALTIEVHDRGAKRQGRWKAGVGMSSMRE
                     RAAEIGGSIEVGPGDTGGRVRARLPLSV"
     gene            complement(34153..34830)
                     /locus_tag="PL014_01005"
     CDS             complement(34153..34830)
                     /locus_tag="PL014_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT55698.1"
                     /translation="MTALRIVVVDDHPVVRGGLRALIGTLDGLEVVGEADDGESAVRE
                     AQLLRPDVVLMDVRMPGGDGIEATRRIRTSVPDTAVLVLTMHDDDATVFTAMQAGARG
                     YLLKGASQDEIVSAVRGVVAGQLVFGPGVASRVLAYFSAGAPHQPPDAQPAPEPFPEL
                     TTREREVLDLLASGRRTAAIAQALYVSPKTVSNHLTSIFAKLEVADRAAAMIRAREEG
                     LGQGSAQ"
     gene            35033..35437
                     /locus_tag="PL014_01010"
     CDS             35033..35437
                     /locus_tag="PL014_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55699.1"
                     /translation="MNTMTTAPSPTRGTTRSKVGLALAGLLSLSNVFSAFGDTAPEGE
                     VGPPAVVLWAGTVLGIVGLVAVVIAWRTGNRGALRVAAGAIIITALTAVPAFFVDVPA
                     WLKAAAAVGVLLTIAAVVLMFSTQRGHATVTD"
     assembly_gap    35451..35550
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            35595..36446
                     /locus_tag="PL014_01015"
     CDS             35595..36446
                     /locus_tag="PL014_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55700.1"
                     /translation="MAIISCPSCLSDNDIHLGADAADGTRTFRCGECEHTWGASVAST
                     RSASDRSPFEMARGRFATASMLSPERAARVVRLKRQFLKNEPLPTPEVDEHRARFQHL
                     FSAEVLATCDPQDLKDFATSSIGGETGTASVYNRAWNKLGADEAAERTRESIDYLLRG
                     PDDVPLEDRLTHLIEEDVDTAMPGFKEPLLTKVLCVVYPERFLPLFSYTAEGTGKREL
                     TESVFGLHLPVKHDATAMQIGRLAVWSNDLLLELVGDGFTGTHHAAAFLLWAKDRAGA
                     RPLSSVR"
     gene            complement(36477..36803)
                     /locus_tag="PL014_01020"
     CDS             complement(36477..36803)
                     /locus_tag="PL014_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55701.1"
                     /translation="MPSTADLPSVKAYGAGSPECAGMSAALMGATQIGGLADQGKVTQ
                     GDFEAAYTTPLLNGIPIDAVPILADMKTASMSLVGLDAAAAQAQLGNSQGALSNLVAT
                     ADAVCS"
     gene            complement(36968..>38508)
                     /locus_tag="PL014_01025"
     CDS             complement(36968..>38508)
                     /locus_tag="PL014_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOT55702.1"
                     /translation="KKDHELDVIVLMPDVGIVVVEVKGGDLRMEDGEWVVGHGKGRRV
                     VHPVDQGRDGKYALRAYIERDPRWKASSRTRVRFGHAIAAPYTDVADDFATPDCPRWM
                     ISGRGDQHDLAGRLHDIAARQESGHRVPTHDDCDLIVEILKGRSLPQRTLLAEADERE
                     SRADRLTIEQATILGVTRLIHRVDVRGGAGSGKTVLALTQAKELTHGRHGQPAQRVAL
                     LCYSVGLASWFKRYMDTVDRRHRPAFVGTFEDLAVYLGVSEFGGRDDTDFWENRLPAQ
                     MAERAGELPDGKRFDAVVVDEGQDFADGWWTPIMRCLRDELNGGVYVYSDENQRVFAR
                     FGRPPVPLVPLVLDHNLRNTRQITETFLPLAPMRMYARGGEGPEVTYVEASADDALDA
                     ADDQIDPLLEDGWRPEDVALLTTGARHPEQVSLQESEGQLGYWRTFWDKDCVFYGHVL
                     GCKGLERKVVVLCVNESTPRERARERLYVGLSRATDKLVVVGPRDVILEMGGPAVARR
                     LGIT"
BASE COUNT         5325 a        13529 c        13627 g         5527 t
ORIGIN      
        1 tcctcgatga ggtctccgaa ctcgctgtcg ccgtcctcgc cgagcggggt gtgcaaggag
       61 atcggctcgc ggccgtactt ctggacctcg acgaccttct cgggggtcat gtcgagttcc
      121 ttggccaact cctccggggt gggctcgcga cccaggtcct ggagcatctg gcgctgcacg
      181 cgggcgagct tgttgatgac ctcgaccatg tgcaccggga tgcggatggt gcgggcctgg
      241 tcggccatcg cacgggtgat ggcctgacgg atccaccacg tcgcgtacgt cgagaacttg
      301 tagcccttgg tgtagtcgaa cttctcgacc gcacggatga ggccgaggtt gccctcctgg
      361 atgaggtcga ggaagagcat gccgcgcccg gtgtagcgct tggccagcga caccacgagg
      421 cgaaggttgg cctcgaggag gtggttcttg gccttcttgc cgtcctgggc gatccaccag
      481 agctcacgct tgagcttcat gtcgagcttc tcgcccgagt tgagcttctc ctcggcgaac
      541 aggccggcct cgatgcgctt ggcgagctcg acctcctgct cggcgttgag cagggcgacc
      601 ttgccgatct gcttgaggta gtccttgacg gggtccgcgg tggccccggc cgtgacgacc
      661 tgctgaacgg gtgcgtcgtc ctcgtcgtcg tcgcgcagga cgaagccgct ggtctcggtc
      721 tccggctcgg caggcttctt gacctcgtcg tcgtcctcgt cgtcctcgag ctcgtcgacg
      781 gcctcggcag ccgcttcggt ctcggcggca gcggccttct tcgcggcagc actgcgacgg
      841 ggaacggtgg tcttcgccgg agcgtcggag ccggccgctg cagcagcggc cttgcgggtg
      901 gtggtggccc gggcggcgcg cggcgcggcg gtcttggccg cggcagtcga ggatgccgtc
      961 gtcttggctg cggcggtctt cgtcgccgca gccttcgtcg cggttgcctt ggtcgcggtt
     1021 gccttggtcg cggttgcctt ggtcgcggtt gccttggtcg cagcggcctt ggcaggtgca
     1081 gccttggtcg cagccgcctt ggtaggcgcg gctttggtcg cagccgcctt cgccggtgca
     1141 accttggtcg cagccgcctt cgccggtgca gccttggtcg cagccgcctt cgcgggtgcc
     1201 ttcttggtcg cagccgcctt cgccggtgca gccttggtcg cagccgcctt cttggccgtc
     1261 gacgccgacg ctgcagcctt cttggtcgtg cgtgccgtgg cagcagccac gccgatgtgg
     1321 gctgggtcca tgacgacctc gatgccgtgc tcgccgagcg cgctcaggac aacacgtcca
     1381 cgcttgggct tgacctcggc gctctgcatg gcgtgggcaa cctgttcacc ggtcaccgac
     1441 cccgtggccg ccccgatgcg aatcagtgtc tgcaaggcct cgtgagagaa ctccggggga
     1501 agtgcgccga gctcggggga tgtgttggac acaggcgaca ccaacaacct ttcgtcgcgg
     1561 aaaaccggga tccgggcgcg cagagcaacg ccggggccgg ctgaggggga ggtcctgtgc
     1621 gacaccgttt cgtccggccg aggtggctgg gccgggggtg tggccaggtg cgctcttcca
     1681 ttataaaacg cgcttcccca ttccggcaca tcgcaccggg tgtcaccggt ggcgatcgac
     1741 gtgctggaga gcggggacag agcaggcgaa ccggctgtga tcaggccttg acggcaagca
     1801 ccgcacaggg tgcgtcgagc aggatccgct gggcgttgga gcccaggatc agcttcccga
     1861 ccggggtgcg gcgccgcagg ccgacgacga tgaggtcggc gtcggtgtcg ttggcgacgg
     1921 cgatgaggtc ctcggcgggc tcgtttccgc gcacgagctg tcgaacctcg agctcgaggc
     1981 cgtcgccacg cagctcgtcg gtgacgcgct ggagctcagc ttctgccgtc tggctcgtgt
     2041 cgtcgtcgta atcccgaccg ccacggttgg agttgatgac gacgagcttg gactggcgca
     2101 acctcgcctc ctcgaccgct cgggagaggg ctgcccgccc ttccttggtc ggtacgaacc
     2161 cgacgacgat ggtcacgatt ttcctcctgt ggggcgggac ggcaatgtct cgtgagcgac
     2221 gttaccgcag ttggggagcc ctcaggcagc gccgagcgcg cgggcgatga ctgctccgac
     2281 ctggtcggtc tcgatgagga agccgtcgtg gccgtaccgc gactcgatca cggacaactc
     2341 gctgcccgtc ctgtccgcga ccatctgctc cgacaagcgc agggggtaca ggcgatcagt
     2401 gctcaccccg gccacgtgca gtgcgccccg gtaggcctgg agcgcgctgg ccactccccc
     2461 gcggcctcgc ccgatgtcat ggctgttcat cgcctcggtg agcaccacgt agctgttggg
     2521 gtcgaagcgc cgggtgagct tggccgcatg gtggtccagg tagctctcga cgtcgtagcg
     2581 gcccccaccc ccgagagggt cctcacccgc cctgggcgac cgaccgaacc gctcgtcgag
     2641 ctcaccctcg tggcggtagg tggtgtgggc gatccgccgg gcgatgccca tcccggtgac
     2701 cggccagtcc tcgtacccgt agtagtcgcc gccccggtac gcaggatcgg agcggatggc
     2761 gagcagctgc ggctggcacc aggccacctg gtcggccgtg gcgtacggcg tcgacgcgag
     2821 cacgagcgtg cgctccaccc gcgcgccgtg gcccgtggcc cactccaggg cacgcatccc
     2881 gcccatcgac ccgccgagca ccccgtgcca ccgctcgatc ccgagcgcat cggccagggc
     2941 cacctccgcg gccacctggt cgcggatcgt gatgaagggg aaccggcctc cccacggccg
     3001 accgtcaggg cctgcggatg ccggccccgt cgtcccctgg caccccccga gcacgttgct
     3061 cgccacgacg aacagctcat tggtgtccag cggcgccccc ggcccgatca ggcccggcca
     3121 ccagccgggt gacgggtgcc cggggccgac ctcacccacg acgtggctgt caccggtgag
     3181 tgcatgctcg acgagcaccg cgttgtctcc cgagggagag agggtccccc aggactcgta
     3241 cgccacggtg acgtccggca ggaccccgcc gcgctcgagc gacaggtccc ccacggccgc
     3301 gaacaaccgg tccccgacgg ggtcgcctgc tcgccagacc gccgaggtgg acctcgtggg
     3361 ggtggctgtc atgacaggta ctcctcgacc atcgcgcttg ccgcaccggg acgggtgggc
     3421 cgggtcatca cccggggcac cccaccgcga ggagggttgc cgcccagcaa accggggttt
     3481 ggcactggga ctcatgaccg gcctccatgc taaaccgcct ctgacctgcg tggcccggtg
     3541 cgtccgaggg ccggtcagcc tgcgcgtccc aacctgccgt cgccgtcctg cgtcaccccc
     3601 tccgtcgagg ggacaggggg aaatcggatg ccggcgtcga tgagtttcgc gagaagcgca
     3661 gccagacggt agtcgggggc gaggtgtcgc gcccgctcga tcgccgcgcg cgcgatcgtg
     3721 ccgtcgccac tggcccaggc cacgtgggcg acgacggtgc agagggccgc cgcctcctgc
     3781 gctgcctcgt ccggcagtga gcggcacagc gcgaccagcc gacgcagcac ctcgtcactc
     3841 ccctcgcccg gttcgccgtc gaagcccatg ccaccccacg agggcatcgt cgactccagc
     3901 atcagcaccg cactgggctg gaggtcggcc aggggcagca ctccgggagc cagccacgcg
     3961 atgacggcat cgcgccaggc gatgtcgcgc aacccgagcg cgagatcggc gacgacagtc
     4021 ggcggcgggt gctgcgcacc accatcgcgg ctgagcccgt cgcggccacc accgtcgcgg
     4081 gcacccccct cggtccgcgg tgggcgcagg agctcggccc acgcgcgcgc cgagcgacga
     4141 cgcaaccggc gccgcggcat cccctcgcgt gccccgatcg cggcggccac tccggtgctg
     4201 acgacggggt cttcctggac cagggcgtcg actgcgcggc gcgagagcag cggggcgcgc
     4261 cctcgtgcga cgtactcggc cacgcccacc acatcggccg ggtccgggag ggcgactccc
     4321 tctgcaggac agcagtcctg ggcacagcgc ggggagtagc gccgtccgtc cctgacgacc
     4381 atgacgtcga gcacctcgac acccgcctgc tcgaggtgtt cggcgaccgc gagcgagatg
     4441 gggacgctct cgtcggcccg gtcctcgaac gaaacgacga cggccgcact ggcgccgtcg
     4501 cggaccaggg ggccggtgag ccaggtggcg acctcggcct cacgaccggg tggtgggatg
     4561 tcgacccggg cgacgagccc gaggagacgg ccacggatgg agacgaccac gacgctgtcg
     4621 cgcgggtggt agccaaggtg gtagggcagg accgacacga tgtcgcccgg ccctcgaagg
     4681 gtgatgtctg gagtcatgac gacaccctcc ccgcacgccg gggagggtgt caggtcacgg
     4741 atgctggcct gtggacaacg ggccgtcaga gcgtcgctgt ggacaacgcc ccgcgcaacg
     4801 atgtgggcgt tggtgccacc cacgcgatca ggagaccgcc tcggccagga gggaccaagc
     4861 tcagcggatg acgtcgccgc gcacggtgtg cgacgacgag ggggtgccgc ccatgaacgc
     4921 gtggcccgag gtggcggcgt aggccggcga gacctgcgcg agctcccacg ccaggaacgc
     4981 ctcggtctcc tcgacgaact gcgtggccag ccacgccatg acgagcgcgt cgtccgcgag
     5041 cccggcgagc atgacgaact cggggatgag gtcgagcggc gacacgatgt aggcgagggc
     5101 ggcgaccatg agggcgagtc ggcccttgct gatcccggtg tactcccccg tgatcgcggc
     5161 ccgcacgagt cgggggaagg ccccggcacg ctcaccgagc gaggggccgc cgggacgcac
     5221 cgcgacgcgg acggcctggt agacggcggc gatggccgcg aagcgcttgg gtgatgccat
     5281 gggttcctcc tggggtcaag ccggtcacgc gtgggcgtgg gtgcaccacg gtgccgttgt
     5341 gctcaacgaa ggacgaacgg cgggtgattc ccgttcccgg accggacgtg acgcacgcct
     5401 cgcccttcag gccgcccttc aggcgaacct caggcgaacc tcagcgctcg atggccgggg
     5461 ccacccgcgg gaactggtcc ttggcgttgc gggcgaaccg gctgagcatg tacgcgtcga
     5521 ggccgccacc cacgacgccg ccgacgacgg gcacgaggcg acccagccgc gcgaaggact
     5581 tggtgccgac ctgggcgagc agccggaagc cgatgccctt ctgcaccacc atgagcaccg
     5641 gcccgggaag gcgctgcacg gccaggttgg acagcgtccc gcccggcacg acgacgttgg
     5701 ccttgcgcag caggtcgtcg gcgtcggcac cgacgagggt gaggagcacg gcggtacgga
     5761 tctgctccga ggacaggtcg tagccgcgca ccttggcgat cgaggccgtc atgcgggtgg
     5821 ccagcaggta gaacgcgagc aggttcaccg gcagcgccag cgggagggtg acgaatccac
     5881 cgacgctggt cacgaagccg gtgccggcgc ccttggtgac gtggctgcgc acgatcgcat
     5941 cgaccgccgc ctcgacgtcg ccgccgttct cccgcagcgc cgcgtcggcc tcgtgggcgg
     6001 ccgagtcgaa ccagcccttg ccgtcgaccc cgaggtcgag gatgttctgg acgaacttgg
     6061 tggactggcg gtccaggacc ccttggtccg cggtgccgtc ctgagccagc tcgagcgcgc
     6121 tcctgacgtc cttgaccgca tcactcgtac ggtccccacc cttgccggga agtgccacgt
     6181 cgtcctcctc gaaactcccc ggcgctgccc gtgcggcgcg ctggcatcga ctgtccatcc
     6241 tgccacgcgg atgtgcgcca ccggccggga ctgacgcacc ctccgcctac gctggcgaga
     6301 tgatccccgg agccgtcgcg gccctcgccg tgacccctcg cggagcgggc cgacggtatg
     6361 cggccctcgt ccacgacggc gggtgcgacg tcattgccct ggagcaggcc gcggatgccg
     6421 tccgctccct cgacgctcgc ctgtcgcccc ggtgggtctg gtgggccgcg tcggatgccg
     6481 cggcaccgct ggtggaggcc ggcatcccgc tcgcgcgggc ctgggacgtc gccgaggcgc
     6541 accggctcct gcacggcggg tggagcgcga ccgcgggcgt gtgctgggcg gccgcgcacg
     6601 gcatccccac cgacaccgcc ccagcaccgc caaccggtga cctcttcgag ttcgcctccg
     6661 aggccgcgcc gctcgctgcc gacgccctgg tcgacggtgc cggccacctg cgcggcgacc
     6721 acgagagctg gctgcgcgac ccggcccacc tcgaggcctg ggcccgcgcc gccctggaga
     6781 ccgcacaccg gcaacacgat gctgcggcag cgacctcggt gcgcctgccc tcgacggtgt
     6841 actcggagtc ggcggccgcg ctgctctgcc tggagctgca gcgcgacggg ctgcccatcg
     6901 accgcgagac cacggaagcg ttgatcgcgg gcgccgccgg accgcggccg accaccgacg
     6961 ccgacgaggc ggcatcccgg cgggttcgtg acgcgcaggt cctgcggctc gcccccggac
     7021 gcgagtccac ggacctgcgc aacccggccc aggtgcgtga gctgctcgcc gcggtcggcg
     7081 tcgacgtgcc gaacacgcgc aagtgggtgc tcgagcccta ccgctcggtc aaccccctcg
     7141 tcgaggcgct gctgacgtgg cggcgtgacg agcggatcgc cacgacctac gggtaccgct
     7201 ggctcgacgc ccacgtcggc gccgacgacc gcctgcgcgg gcgctggacg gcgtgcgacg
     7261 gcgcggccgg gcggatgacg gccgagaacg ggttgcacaa cctgccggcg gccctgcggc
     7321 cgggcgtcgc agcccacccg gggcacgtgt tcgtgcgtgc ggacctcggc cagatcgagc
     7381 cgcgggtgct cgccgtggtg agcggggatg ccgtgttcgc ggcggccacg caggccgacg
     7441 acctctacgc cccggtggcc cagcgcctcg gggtcgagcg accggtcgcc aagattgccg
     7501 tcctcgccgc gatgtacggc cagcgcagcg gggccgctgg tgaggcgctc aaggggctgg
     7561 agcgggccta ccccgtcgcg atggggctgc tggatcgggc gtacgccgcg ggggtgcgcg
     7621 gtgccccgct gcgcacgtac ggcgggcggc tcatcccgac gggcagcgtg ctgaccgggg
     7681 ccggggtcgg ggctgatccg gcgctggacg ccgcccgggg acggttcgcg cgcaacgcga
     7741 tcatccaggg cgcggcggcc gagctgttca aggcctgggg cgcgaccgtg cgggcgacca
     7801 cccgcgagct cggggcgcag gtcgtgctct gcctgcacga cgagctgctc gtccacgcac
     7861 cgctcgagca cgccggcgag gtctcggcgc gcgtcgaccg agcgttgcag gacgcgtcac
     7921 gccgctgggc tggcggggcg ccggtgcggt tcgtcgcgga cacctcggtg atccaccggt
     7981 ggtccgaggc caaggactga cgtcccttcc cccggccaga tggtctaccg gataacccga
     8041 tgaaccccac atcgcccgac aagagctgtc gcgggacgtg agggtttgtc gggtggagcg
     8101 tggccgtcag gcggtgtcgt cgtccgcgac gtggcgcagg aagttctcga agtcggcgcc
     8161 gagctcgtcg gccgacggca gctgcgaggc ctcggtggcg agcaggctgg gccgctcctt
     8221 gccctcgagg taggtgtcgt actgctgctc cagcgcgctg acgaccgcgc gggcgtcatc
     8281 gttgccctcg acctcggcag cgatggcctg ctggttgctg cgggcacgca gcacgaggtc
     8341 gtcgttgggc aggttcagac cggtcacgtc gaccacggcg ttcagcgcgg cgagcgcccc
     8401 ctcggcccac tcggcaccgg cgaggtagtg cggcacgtgg atcgcgaagc ccaccgcctg
     8461 gtggcctgat tcgccgagcc gcatctcgag cagggccccg atggagcctg gaacctggac
     8521 gcggccgaac ggcgagatgc tgtcaccgat cagcgaggtg tcggtcgcgt gggccgtcat
     8581 gccgacgggt cgggtgtggg gaaccgccat ggggatgccg tgggtgtgca ccacgagcgt
     8641 gatccccagg cgcacgacca gctcgcggac cgcctcgatg acgcgctccc actggtagtc
     8701 gggctcgggc ccggtcagca ggtagtacgt ctgcccgtca cggtcggtca ggcggtgcag
     8761 cagcatcgtc gggtcgtcgt actcgaccca ccggttggtg tcgaacacca tcgctgggcg
     8821 gcggccgcgg tagtcgagca gctggtcgac gtcgaaggac gcgacgacct cggggtcacc
     8881 ggtctcgagg aggtgaccac cgaggacgga ctggatctgg ccggcgtcga cgaacgcccc
     8941 gagggtgacg acgagcacgc cgggccgggg cacggtgagg tcggcgtcgg tctcgaaacg
     9001 atagagcgca gaggggttca gcacggcacg gcctttcgcg ggtggagcgt tcctgctgtg
     9061 ggcaacgtcc acgcgccccc gcaggattcc cgcggtcggc cgtcagcggt cgatggcggc
     9121 gatcgccgcg cggacgcgtt tctcggacac cgggcgcggt gtgccgaggg cctgcgcgaa
     9181 cagcgacacc cgcagctcct cgatctgcca gccgatgtcc accacgtcgg cggcaccgcg
     9241 ccgcaccggc ggcagcgact cgagcaggtc ggcatatgcc gcctcgaccc cgtcgatctg
     9301 ggcctgcagc accccttcac gggcattggt cgccgcccgc tcgagccgat gctgagccgc
     9361 gcgcaggtac cgctgcacgt cgcgcagccg ggcaaccccc gtcgcggcga cgaagcccgg
     9421 tcgcacgagg ctgtcgagct gggcacgcac gtcggccctc gtggccgcgg tccgcgccga
     9481 ggctgcgccg cgttcgaggg tcgccagcgt ggcgcggacc tgagcggcca gggcgagtac
     9541 cggctccacg gcctcgacga cgacgagcac ccgggtggcg gcgtgggtgc gcacggctga
     9601 cagggctgcc tcataggccg cctgcgtgcg cacctcgccg ggcacgtgct cggcgacgat
     9661 gtcgtcgacg gccgcatcga ggcagtcctg gagcagtgcg ggcacggagc cgtgcgggtt
     9721 gtcggcgagc gccagcttct gggcattgct caaccgggcg agcacccgct tccacggagc
     9781 ggtcgtgccg agcagcagca accggcgaac cccggcccgg tgcgctgccc gcgcggcacc
     9841 ggcatccggc aacacccgca gcgcgacgct cgtcccctcg tcgaccagcg ccgggaaccc
     9901 ctgcacggtc agcccgccgg agcgcccctc gaaggtcgcg ggcacgtcgc cgaccgacca
     9961 cgtcgtgagg ccggttcgct ccagcgacgc gccggcacgg gccatgccgg cgcgcaccgc
    10021 tcccccggcc tgctcgcgca gggcgtccag gtccttgccc cgggcgacga cccggcggcc
    10081 ggggccctcg atgctgaacg tgatccgcag gtggtcgggg acccgctcga cgtcccactc
    10141 atccggggag atgcggatgc cggtgcgcgc gtggagcact cgcgcgagtt ccgcggtgag
    10201 cggcccccgc gacgggtcgg cctcggcgag cgcggcggct gcgtggtcgg gggtgggcac
    10261 gaagtggcgg cgggtggcct tgggcagtga cttcagcagg gcgacggcga ggtcgtcgcg
    10321 cagcccgggc acctgccagt cgaacccgtc ggaggtcacc cggttgagca cggcgaccgg
    10381 cacgtggacg gtgacgccat cggcatccgc cccgggcgag aactggtagg tgacgtcgag
    10441 ctcgacgtca ccctggcgcc aggtgcgcgg gtagtcggcc gcggagacct gctcacctga
    10501 gtctgcggtg aggatctcct cggtgagggt cagcaggtcc gggcggcgcc ggcgctcccc
    10561 gcgccaccag cggtcgaagt gccgctcgct gacgacgtcg ggtgggatcc ggctgtcgta
    10621 gaacgcgttc agcacctcgt cgtcgacgac gatgtcgcgg cgccgggccc gttcctcgag
    10681 ctcggcgacc cgggcgaggg tggcgaggtt gcgcttccag aagtcgtgct gggggtccca
    10741 gtccccctcg acgagcgccg acctgatgaa gatctcgcgg gcggcctcgg ggtcgatacg
    10801 ggcgtactgg atggtgcggt cggtgaccag cggaaccccg aacagggtga cccgctcggt
    10861 cgcgaccgcc gcaccggccc gacggctcca gcgtggctcg ctgacggtgc gggtgacgac
    10921 gtgggcgccg gcctcctcgg cccactccgg ctcgatggcg gcgttgaccc gcgcccagag
    10981 ccggctcgtc tcgacgagct cggcgctcat gacgacgtcg ggctgcttac ggaacaggcc
    11041 cgaccccggg ctgatgccga accgggcgcc acgtgccccg acatactcgc gcttggcctg
    11101 gtcgcgcacc ccgacgtgcg agagcagccc ggcgagcagc gccttgtgga tggtgtccca
    11161 gtcgggctct gcgtccggtg ccgaggtcgc gacgtcgggg tcgatgcgca ccgagcggca
    11221 ggccgcccgc agctgggagt gcaggtcctg ccactcacgg atgcgcaggt agtgcaggta
    11281 ctcggccttg caggtgcgtc gaaatgcgct gtgcgacagc gccttctgct gatccttgag
    11341 gtagcgccag agggtgtgca gggcctcgaa gtcggagcgc tcgctcttga accgggcatg
    11401 cgactggtca gcctgggcct gcttgtcgac ggggcgctca cgcgggtcct ggatcgacag
    11461 ggccgagacg atgacgagca cctcgcgggt gcatccgaac ttgcccgcct cgatgaccat
    11521 gcgcgccagc cgcgggtcga gcggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnngcaccg agttcttcga ggagccggac cccgtcggcg atctgtcggg ggtcgggtgg
    11701 gtcgacgaac gggaagcggg cgacatcacc caggcccagg gcggtcatct ggaggatgac
    11761 cgaggccaac gaggtgcgct ggatctcggg ctcggtgaac tcggggcggg cctcgaagtc
    11821 ctcctcggag tacagccgga tgcacacgcc gggggcgatg cggccgcacc gaccggtgcg
    11881 ctgcgcggca ctggcctggc tgaccggctc gatcggcagg cgctgcacct tggtgcgctg
    11941 gctgtaccgc gagatgcggg cggtgccggt gtcgacgacg tacccgatgc cgggcacggt
    12001 cagcgaggtc tccgcgacgt tcgtcgccag gatgatccgc cgcccgctgc cccggtggaa
    12061 gacgcggtgc tgctcggcgg ccgagagccg ggcgaacagc ggcagcacct cggtgttcgg
    12121 cagcttcagg gcgttcaggg catcggcggc gtcgcggatc tcgcgctcgc cggagaagaa
    12181 ggcgaggatg tcggtcgcct cggggctcgg cggggtctcg gtccacagtt cctcgacggc
    12241 ctcgacgacg ccggtcacct ggtcgcgctc ggcgacgacg tggcggccct cggggtgcag
    12301 ctcgaccagc ggccggtagc gcacctcgac ggggtgggtc cgcccggaca cctcgatgac
    12361 cggcacctcg cgcacctcgc ggccgtgctc gtcggtcgcg aagtgcacgg cgaagcgcac
    12421 cgggtcgatc gtggccgagg tgacgatgac ccgcagctcg ggtcggcgcg gcagcagctg
    12481 cttgaggtac ccgaggatga agtcgatgtt caggcttcgc tcgtgggcct cgtcgatgat
    12541 gatcgtgtcg taccggcgca ggtcgcggtc gcgctgcatc tcggcgagca ggatgccgtc
    12601 ggtcatcacc ttgacccggg tcgcgtcgct gctgtggtcg gtgaaccgca cctggtagcc
    12661 gatgaggtcg ccgagctcga cgcccatctc ctcggcgatg cgttcggcca ccgagcgggc
    12721 ggcgatccgc cgtggctggg tgtgcccgat ctggccggtc gccccgaggc ccagttcgag
    12781 gcacatcttc ggcagctggg tcgtcttgcc ggagccggtc tccccggcga tgacgaccac
    12841 ctggtggtcg cggatccccg cgaggatgtc gtcgcgacgc tcgacgaccg gcaggtgctc
    12901 ggggtagcgc agctcagcag gatccaccgc ccccggtgca gagacgtcga cgtgctgggc
    12961 aggcatccca ccagcctaat tcgcctcctg cctcccccgt gccgtagcgc cccgcatcgc
    13021 gaggtgatgc gcaaccatgg ggcgatgacg gcatccgaga acccagcaac cgacggcggc
    13081 tcactcgtgg accgcacgaa ctatgagggc gccaccttcg agcgcgcgaa cttcaagggc
    13141 gcgaccatcc gcttctccga cgtcagcggg atgacgatgc gcagcgtgga cgtcggcggg
    13201 ctcgacatcg acagccacga cctggccttc ggcttcctgt tcgtcaacgg ggtcgacgtc
    13261 gtgccgttgg tcgaggccga gctcaaccgg cagttccccg gccgggagct gcagaaggcc
    13321 accaccccgc acggcctgcg cgagggctgg gtcgccgtcc aggcagcctg gcagcacacg
    13381 gtcggcgaca ccccggcccc gctcgtcgac gcccacgtcc cggacgagtg gtcactcgcc
    13441 cagaccctgc gccacctcgt cctggccact gatgcctggc tgcgcagcgc gatcctccag
    13501 gtgcctgcgc ccttccacga gatcggccag ctgttcaccg gtgccgaggc catgggggtc
    13561 gacgtctccg ccttccgcga cgacgtgccc ttcgacgagg tgctgcgcgt gcgtgccgag
    13621 cgccagcaga tggtgaccgc cttcctcgac gaggtcaccc aggagacctt ggccgagccg
    13681 cgcaacgacc cgtggggaga cgaggactgg cacccgagcg tcggcgactg cgtccgggtc
    13741 atcctcgagg aggagtgggc ccacctgcgc tacatccggc gggacctggc cctgctgacc
    13801 cagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccgcc accgcctcca
    13921 tagctgcccg gactcgtcgg ctcgccggac ggaccaggac gcggcggcgg ggcccctccc
    13981 actactccgc gggccgcggc tacctcgccg actgagcacg gtgcggctcc tgctggggca
    14041 ccaccgtggg gagcaccttg ggtgcgacct tcacccccag gtcgagggtc gtcccggcgg
    14101 catacatctt ctgagctgca ggaccgttgg cgctggcccg ccgccacccg tcgaggaaca
    14161 cctggtccgc cttggcctgc gctgtcgtgc gcacccgggc gaccacctgc tcgcgcgaca
    14221 acttccagac cgaggtggtg cgcaccgggc gaggtgtgac cccgatgaac ctggtcgggg
    14281 tggcgcgatc atccgcctgg acacggaacc gtgcccggat ggcgtcgcgc ttggccctcg
    14341 cctgcaccgt caccccgact gcaagccgct gctgggtcgt gagcctgcgc gtgcgcgtga
    14401 agaccgaccc ggatgccgac ttcgtggccc ccgcggttcc cgcgctcttg agcgccccgg
    14461 ccacattgcc cgcggccgcg atcgcgaccg tggtcccgag ctcggaccac gccccctccc
    14521 cgtaccccac gagcaacgtc gtgtcggcag ccacactcag gacatcaccg gcagcgccga
    14581 ccggacgcag gtacgggacc accgtcgcca ccgtgcccac cgccgccagg tcgcgaccga
    14641 ccgtctggct cccggccacc agccggtaga gcgccgagtc ggtcacggca tcctgcgtca
    14701 gtgctcgcac cgaggccgag caccgagcat ccaccgcacg gaaccgctcc atggctgcgg
    14761 catgtcgggc ccgggcacgc tccctgatgc gcacctggtc acgctggagg atgcgcaccg
    14821 ccacgacgtc gggcgaggtc tcgtccgcgc cggcactgat cagggcgtac gcccggtcct
    14881 ccagcaacgc gtacgtgccc tgagcctcgg agtcgtcacg gatcgccccc tcgacgaccc
    14941 gttgcgcctc ctccatggca tcggcgatct cgcgcaacgg caccgcagcg ccgccgagcc
    15001 gccgcgccac ggcatccaac agcggcggcg cctcagcaag cctggccccg aacgcctcac
    15061 ccgctgaact cacccacgtc gcgccaccct tcagcgccac gagcacggtg gtgacgtcac
    15121 cgacacgacg cgacgacgcg gtcagccggt cggcgaggtc gcgcacgccg ctcgggcagc
    15181 ccgacaacgg ttccaggatc acgactgggc accgccgaac agtcgcgtga ccccggcctc
    15241 ggtgtcgacg tacacggtgc cggcggacac cgccgcctcc ccgagcgacc cgagcgccct
    15301 cccgagcgcg atccgctgcc ggcgccaccc cccgagcagc tcctcgacgg cagggccgca
    15361 ctcccccggc ccgagcacca tgcgctcgag cgacgggtcc tgcatcagca ggaagtcggc
    15421 gcccacctcg gtgagcgaca cccccagctc ggccacctgt gccccgtcaa cccggatgat
    15481 cgtcatggcc accacggtag gcggccatga cgatcagccc ggaaagttat ccacaggcag
    15541 gcgaggggcc cgcctgcgcg cggtcccgcc tactcggcgg ccgtggcgga agccccgcca
    15601 gcagcggcgg cgaacccggc atccaggtcg gcgaggatgt ccgcgatcga ctccagcccg
    15661 accgcgaggc gcaccaaccc gggcgtgacg ccagcggcga ggcggtcctc gtcgccaccc
    15721 tggctgtggg tcgtggatgc cgggtggatc gcgagcgagc gcacgtcacc gatgttggcg
    15781 acgtggctgt gcagcgtcag cccctcgacg aagcgctgcc ccgcctcgag accaccggcg
    15841 atctcgaacg cgagcaccgc accggcaccc ttgggtgcgt acttgtcggc cagcgccttg
    15901 ctcgggctgc cgtcgagcga ggcccagcgc accgactcca cctgcgggtg ggcggccagg
    15961 tgggccgcca ccgcgcgagc gttctcgatg tgccgctcga cgcgcaggct gagcgtctcg
    16021 atgccctgcg cgatgaggaa ggcgttgaac ggcgagatcg ccgacccgag gtcgcgcagc
    16081 agctgcacgc gcgccttgag gatgtacgcg aggttcgcgc cgagtgcacc gccgacaccg
    16141 aggtcacggg cgaacaccag cccgtggtag ctctcctcgg gcgtgttgta gttggggaag
    16201 cgctccgggt cctggccgta gtcgaaggtg ccaccgtcga cgatgacacc ggcgatcgag
    16261 gtgccgtgac cgccgaggta cttcgttgcc gagtggatga cgacgtcggc gccccactcc
    16321 agcgggcgga tgaggtaggg cgtcgcgatg gtgttgtcga cgacgagcgg cacaccggcc
    16381 tcgtgggcga ccccggcgac cccggcgatg tcgagcacct cggccttggg gttggcgatc
    16441 gtctcggcga agaacgcctt ggtgttggga cgcacggcgt cgcgccaggc ctgcaggtcg
    16501 gcctggtcct cgacgaaggt cacgtcgatg ccgagcttct tgaaggtgta cttcagcagg
    16561 ttcaccgtgc cgccgtagag cgccgagctg gcgacgatgt ggtcaccggc ctcggcgatg
    16621 ttgagcaggc cgagcgtggt cgccgcctga cccgaggaga cgaggagtgc cccgacacca
    16681 ccctcgagcg cggcgatccg ctgctccacg gcatcctgcg tcgggttcat gattcgggtg
    16741 tagatgttcc cgaactcctt gagcgcgaac aggtttgccg cgtgcgtggt gtcgttgaag
    16801 acgtaggacg tcgtctggta gatcggcagg gcccgggcac cggtggtggg gtcgggcgcc
    16861 tgaccggcgt ggatctgcgt ggtctcgaaa ccccactggg acgggtcagt catggcattg
    16921 cctctttcag cgagcgcttg cgagtcggcg ggcgccgatc gccaggtctt cacccggggc
    16981 accccaccgc gggtggaggg ttgccggcca gcgagccggg gcttgtcgct ggcactcatg
    17041 acctgccggg actatacgac ggcatccgga tgccggtcac gcggcccccg cgtccatctc
    17101 acctcgcgaa cggggcagga tggctccatg acccttcagg cagacgagta ccaggccgct
    17161 cccggacgtt acgaggcggg gatgccgtac cgccgcgtcg gccgctcggg gctggacctc
    17221 ccggcgatct cgctcgggct gtggcacaac ttcggcgacg acgtgccgct ggagcgccag
    17281 caggccacgc tgcgccgagc cttcgaccgg ggcgtcacgc acgtcgacct cgcgaacaac
    17341 tacggcccgc cctacggcag cgccgagcgc aacttcggga cgatcttcgc ccgcgacttc
    17401 tcccgctacc gcgacgagct gatcatcagc tccaaggcgg gctacgacat gtggcccggc
    17461 ccctacggcc agggcggcgg ctcgcgcaag tacgtcatcg cgtcgtgcga gcagtcgctc
    17521 aagcgcatgg gcctggacta cgtcgacatc ttctacagcc accgtttcga cgagacgact
    17581 cccctcgagg agaccatggg tgcgctggac tcgctcgtgc gctctgggaa ggcgctctac
    17641 gtcggcatct cgtcgtactc gggtgagcgc acccgcgagg ccgcggcgat cctgcgggag
    17701 atgggcaccc cgctgctcat ccaccagccg tcgtactcga tgctcaaccg ctggatcgag
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acaccgagac caccgacacc
    17881 tacatcccca gcccgtcgga ctgggtccgc gagcaggtcg agaccatcga ggcgaccggg
    17941 gacacccgct cggtcgacat catgggcctg cccgtcgtcc tgctgacgat gcgcggcaag
    18001 aagaccggtg cgctgcgcaa ggttcccctc atgcgggtgg agcacgacgg ggtgtacgcc
    18061 gcggtggcga gcaagggcgg cgctcccgag cacccgcagt ggtacgcgaa cctgctggcc
    18121 aacccgaccc tgacgctcat ggacggcacg gcctcgtggg atgccgtcgc gcgcgagatc
    18181 acgggcgagg agcgcgagct gtggtgggaa cgctgcgtcc aggcgttccc gaactacgcg
    18241 gagtaccaga ccaacaccga tcggaccatc ccggtgctgc tgctggagcc ggtgtcagcg
    18301 ggctgagctc acgcaggaac agcgcggtcg acccggcgga accaccagag gccgagcacg
    18361 ggcagcacga gcgggacgaa cccgtagccc tgaccgaagt gtgaccacac ggtcttgtcg
    18421 gggaaggcgt cgggcacgag gaaggacgcc gtcccgacga cgagcacgcc gacgagctcg
    18481 accgcgcagg cgatgactcc gacgcgcacc caccgtccgc cccgggcgag gcaccaggtg
    18541 gcgagcacgt agacggccgc cgcgagcgcc gagagcgtgt aggcgagcgg cgccgccccg
    18601 aagtcggtcg tgatctggag gatcgagcgg ccggtggcgg ccagggcgag cacgccgtag
    18661 acggtcacga ggatgacgcc gggcccaccc agtgcccggc ggtaccctcg gggctggccc
    18721 ggggcgtcgc ctcgaggctg gccggggtca cgttcaggca ttgaggttcc agatctgctg
    18781 cagccgcacg gtcatgaccg cgacggcgaa cgcgccaacg gccagggacc ccatggccca
    18841 gaccgtcttc tccttgatcg tcagcaacac ggtgctgatc gggatgacgg gcagcgacac
    18901 caggtaggcc acgaacgtcg cacggtccgt ggagtcctcg atctccccga gcccgacgag
    18961 cccgagcacg agctggacga tcaggccgat ctccagcagg gccgcgagca acagcacccg
    19021 gtcgtcgatg agccggtccc gtacggcgta gaagcccgag acgacgagca gcacggccgc
    19081 caggcccacg acggcatacg tcagcggcgc gatcacgtgt gtcagcctac ccgcgggtat
    19141 ggtccccaca tgaccagtgc acagctcggc gacgtgacca tcgagttcga gatcgacggc
    19201 cccgacgacg gcatcccgat gctcctcgtc atggggctgg gcgcccagct cgtcgcgtgg
    19261 ccgaccccga tcatcgacgg cctcgtcgag aacggcttcc gggtcatccg gatggacaac
    19321 cgcgacatcg gcctgtccac gaagacccag ggtccggccc cgacgcgggc agacctgctc
    19381 aaggccttcg cccaccgtcg ctacgcccag tccgactacc tcctggccga catggccggc
    19441 gacgcggtcg cgctgctcga ccacctcgac atcgaggccg cccacgtggt cggtgtgtcc
    19501 atgggcggga tgatcgccca gcagctgacc atcgaccacc cgacgcgcgt gctcagcctg
    19561 tgctcgatca tgtcgaacac cggctcgcgc ctgcacggga cggtggctcc caagctgctg
    19621 ccgaccatgg cgatgaccat gaccaccccg cgctcgaccg acccggacga ggccgagcgc
    19681 ctcggtgtcg agagcttccg cctcatcgcc gggccgcact acgacgccga cgagatggcc
    19741 gtcatggttc gccgggccat cgaccgggac atcaacccgc tcggcaccgc tcgccagctg
    19801 ctcgcgatcc aggccagccc cgaccgcacc cccgggctgc gcacgatcac cgtgccgacc
    19861 gtggtcatcc acggcctgct cgaccagctc gtcctgccga gcggtggtat cgcgacggcc
    19921 cgggcgatcc cgggctcacg cctggtgatg ttccccgaca tggcccacga cctgccgggt
    19981 ccgcgccgcg ccgagatcgt cgacgagatc gtccgcaacg ccgcccgcgc gaaggtcagc
    20041 acctcgtcgt gagtgcggaa gtggtcgcca gggcgaccac ttccgcactc acggctcggg
    20101 tctacgggcg ctcgagggcg tccgcgacgg catccacgag catgccgacc tcgtcagccg
    20161 tgatgaccag cggcggggcg atgcggatgg tggacccgtg ggtgtccttg gcgagcactc
    20221 cgcgccgcat gaggcgctcg gagacctcac ggccactcgg accgccgggc tcgatgtcca
    20281 gcccggccca caacccacga acccggatgc cggagagccc gtttccgtgt cccgtgcgca
    20341 cgagcggggt gaggagttcg gtgagcaggc cgccgagctc ggtggcccgt gcctggaact
    20401 ccccctcggt gagcatggtg acgacctcgt ggccgatcgc ggcggcgagc gggttgcccc
    20461 cgaaggtcga gccgtgcgac ccgggggtga tgacctcgag gatctcgcgg ttggtcgcga
    20521 tggccgagac cgggtagagc ccaccgccga gggccttgcc catcacgtag atgtcgggaa
    20581 cgacgtcctc gtggtcacac gcgaaggtgc gtccggtgcg ggcgagcccc gactggatct
    20641 cgtcggcgag catgagcaca ccggcctcgt cgcagatctg gcgcacggcc cgcaggtagc
    20701 cctcgggtgg gatgacgacg ccgccctcgc cctggatcgg ctcgaggagg accgccacgg
    20761 tgaactcgtc gaccgcctgg cgcagcgact cgacgtcgcc gtaggtgacc gtgcggaagc
    20821 ccggggtgta gggcccgtac tcgtcgcggg cgaccttgtc gtcggagaag ctgatgatgg
    20881 tcgtcgtgcg gccgtggaag ttgccctcca tgacgatgat gttggcctgg tcctcgggca
    20941 cccccttgac gcggtacccc cagcgacggc tgaccttcag ggcggtctcg acggcctcgg
    21001 cgccggtgtt catcggcagg accatgtcct tgccggtcag ctcggccagg gcctgcgaga
    21061 acgggccgag ctggtcgttg tggaaggccc ggctcgtgag ggtgagccgc gagagctggt
    21121 cgtgggcgcg cgccagcagc cgcgggtgcc cgtggccgaa gttcagcgcc gagtacccgg
    21181 cgaggcagtc gaggtaccgc ctgccgtcga catcggtcac ccaggcaccc tccccgtggg
    21241 agagcaccac cggcaacggg tggtagttgt gcgcggcgta ccgctccacc tgggcaatgt
    21301 gtgcggcggt ctgggcgctg cccacgtggc tggtgctgct gtctgacgtc atcgtcgcct
    21361 cctcgctggt gagcctagcg atcggcgagg ttcagcgcgc gggtggggcg gacggcatcc
    21421 ggtcgtggcg gtccaggtgc gccggttcag gtgcgccggg tcaggtcgag ctcggggcga
    21481 ggacgaggta gccctgcgcg acgaggatga tgatgagcgc accgatcttg tcggggccca
    21541 gcaccgtgcc gaagtgcacc cggtcgacga tgatgatgcc gacctgcagc agcacgatcc
    21601 agcccatcgt cacgaccgcg agctcggcgt actgcagcga cgaggcgtag aaccagaaca
    21661 gggcgaccga ggcgatcgtg ccgaaggcga tggcggcgat gctctgcctc tccgcccact
    21721 ccttggcggc gatcgcgccg agcaggtcga gcccggcgag gacggccatc gccacggtgg
    21781 cgacgacagg caccggccag gatgccggga acttcatcag cgggacgacg tcgagcgcag
    21841 ccatggcaga cctccggggg gtcgcggtca cgcctgtgcg tgacctcaac accaaggagg
    21901 cctcacgccg cccggtgata cacccggccg aggatccagc tgtcaggtca ttcagccgtc
    21961 gagccattcg gccaggtgag gccttcagcc gccgggccaa tctgccgtcg agccattcag
    22021 ccaggtcaga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtgactca
    22141 gccgtcaggt gactcagtcg tcaggccagc cacatctcgc cgacgcggtc ggcgatgcgc
    22201 tggccgtggg cccgagccgt ctcgaaggtg tcggcgccgc ggctgaagtc gagtgggttc
    22261 gcacccatcg cccacaggtc gcgggcgtcg ggaacgacga ggtgggtacg ggatgccggt
    22321 cgcagcgagc ggagctggcg ggccggcagg tcgcgcaggt gcgaggccac ggggtagggc
    22381 gacagcgcga gcacgcgacg gtgcccgacg gccaggtcgg cgttcgccac cgtgcgcagg
    22441 ccgccgtcga cgtagcgccg gccaccgacc tcgacggccg ggaacacccc gggcacggcg
    22501 cagctcgcgg caacggctcg ttcgagcggg acaccgctcg agttgtcgaa cacgaccgag
    22561 gttccggtgc gcgcgtcgac agccgtgatg aacaggcgct tgtccggcca ctccttgccg
    22621 acgagtccga gaccgatggc cttcagccac tcctcctcgg tggtcgtgcg ggcggcgagg
    22681 gccgcctggc cgaccagggc gcggccacgc ttcttgtcca gcgacatctg ggcgcgcagg
    22741 tacctcgagg cgtcggccgg cccgaggcga ccgatctcgg cgatctgggc accctcgcgg
    22801 atccgggcga aggcgagcga ctcctgggtg ccgacccgca ggtgggcggc gaccatggac
    22861 ccggctgagg tgccgatcat cgtgtcggcc tcgcggacgt cgacaccgcc gtcgcgcagc
    22921 cccatgatga cgccggcctc ccacgcgatg ccggtcgctc caccgccggc gagcacgaac
    22981 gcccgctcgt tgtcggtggt cggcggctgc cagccggcga actcgtcgcc gccgtcagtg
    23041 gtggcgctca cggcgacgct cccccgcggt gcgtccccgt ccccggacac gcccgaggcg
    23101 ggaccgatgc ccccacccgg ggtctgttcg agggccagtt gctgcacctg tggcgcgatg
    23161 ttactcacgg gtacattttg ccacgccacc ggtgtcaggc gcacgtccgc acgaggtgag
    23221 agtgctcacg ccgagttggc ctgccctcgc cggtttcgag gcgagcccac gacgcaagcg
    23281 caccgggcgc atcacaccga gcgcatcaca cggggccctg tagacacacg cagcacatca
    23341 cgcggagccc gtcacaccga gcgggctgcc gtcgccggct cgaggcggac cagctcgagg
    23401 ggcaggctca cgagctcgtc accgacccgc acctggcagc gcagcgtgcc cacgtgctcg
    23461 acgtcgccgg aacgcccacc ccacggcccg gacagcacgt gcaccacgtc accgaggtgg
    23521 accggcgggc gtcgcgtggc ggtcggccgc acggtctcag caagcgcggc cagctgctcg
    23581 gcgtacgacg ggggcagcgg cacccggcgg cctcggtacg tccaggtgaa caggtgctgg
    23641 gggtcgaacc gcgtcgaaca ctgcccgcag gacatcaacc gggtcgggcc tcggtggcga
    23701 gacttctcgt gacccgccgg gcagcgcccg acccagtcct tgggcacgcg aggggcctcg
    23761 ggcgagacgt actgccgccc gctggaaccg atctcgcgag ccttggcctg ccacacggca
    23821 tcatgaccgt gccggggccc gacgagggcg tgggcgatct cgtgcaggat cgtgtcgagc
    23881 acctcgctct cgtcgtgcag cgtggtcaac gggccgctga taccgatctc gcgccgggag
    23941 tagcggcaca ccccggcccg ggtcttggcg cggtcggcga cgatcgtcca gtcgtcgagg
    24001 ccgtgttcgc gcagcaggcg gcgacccgtg gccagtgccc tggtgatctc catcagccct
    24061 cctctcgcga ccggtcgttc cctgcggtgt tccctacagc gtccctgcgt ggcccgtgcc
    24121 ctgatgtgct gtgctgtgct gtgctgtgct gtgctgtgct gtgctttgcc cccgacccta
    24181 ggccgaggca ctgacagccc ctcgccacga cgcgcgccca cgcccggcca ccgctgcgct
    24241 gcacactgat ctgtgggggc tgcgcgctca cgcccacagg tgcagactgt ccaccgtgca
    24301 cgtgcccacg ccgaccctgc ccatcggcct tcgcctgctg cgcaactact ccggtcgcca
    24361 cctcccccag gacgtcctgg ccgggctgct cgtcgcggcc ctcgccgtcc cacaggccct
    24421 cgggtacgcc gtcgtcgcag gggttccggt gcaggtcggg ctctacaccc tgccgccggc
    24481 actgctcgcc tacgccctgt tcggctcctc gaggctgctg ttcgtcggac cggtctccac
    24541 ggtctcggtg ctctcgggct cgatcgtcgg cgctctctcc gacggggacc gggcactggc
    24601 catcaccctc acggccaccc tcgcgctcac cgccggggcc gtgctcgtcg ccgtgggctt
    24661 gctccggctg ggatgggtgg cccagttcct cagcgagccc atcgtcaccg gtttcgtcgc
    24721 cggcctcgtc gtgctcatcg tcatcggtga gatccccgcc ctggcgggag tgagcgcccc
    24781 gagcggcggc atcttccagc gcatcgacga gacggtgcgc aacctcggtg acttccacgg
    24841 cgtcaccctc gccgtcggcg cgagtgcgct cggcgtgctg ttcgtggggt cgcggctgct
    24901 gcccagggca ccctggtcgc tcatcgtgct cgccggcggg atcgccgtgt cgaccaccgc
    24961 cgatctggcc tcacgaggcg tacgcgtcgt cggtgaggtg cccacgggcc tgcccctgcc
    25021 ctcgatgccc gtggtcgacg tgcagctgtg gagcggcatc atcaccggcg gcatcgccat
    25081 cgctgccgtc ggcatcgccg aagggctggc tgcggttcgc acgttcgccc cgaccggagc
    25141 catcgacccc gagagcgaca accgggagtt cgtcgggcac ggggcggcca acctggcctc
    25201 cggcatcttc ggtggcatgg gggtcggcgg gtccctgtcg aagacggcgg ccaacgcccg
    25261 cgccggtgcc ttcacccagg tcagtggcgt cgccgcagcc gtcatcgtcc tgctcttcgt
    25321 cgccttcgcc gccgcgctgc tctccgacct gccccgggcc gtgttgtcag ccatcgtcat
    25381 ccacgccgtt tggggcctgg tcagcccgtc gaccttccgc cgctacgccg cggtgcgccg
    25441 caacgacttc gtcgctgccg tcgtcgcctt cggcggggtg ctgttcctcg gcccgctcaa
    25501 cggtctgctc cttgcgatcg cccagtcgct gctcgggctg gtctaccgct cgatgcaggt
    25561 gggcgtcgac gagatgggcc gggtcgaggg cgagaaggcg gcctggggct cggtcggaaa
    25621 cgactccagc cgccgcacct accgcggggt caccgtgttg cgtcccgacg ggccgctctt
    25681 ctgggccaac gcgacctccg tcgtcggcca catcgagatg gccacccggg cccgccaacc
    25741 gctctacgcc gtcgtgctcg atctcgaggc caccaaccag atggacacga cgaccgccga
    25801 gcggctcgac ggcctgctca cccggctgcg tgccgagggc accgacctct acctcgtgcg
    25861 ggtgttcggc aacgtgcgtg acgtgctctc caagtccggt ttcctggaca ccctcggccc
    25921 cgaccacgtg tggcactcga tcgcggccgg ggtcaaggcg gcccgggcag cccgcgctgc
    25981 cgggatcgat ccggccaacc ccttcccgac cccggacgag gtcgaccccc tcgacgagcc
    26041 cgatgagctc gacgaggggg ccggtgagca gatcgcctcg aaaggcgagc gcccctgatc
    26101 cctcccggcg gatgccgtcc gctgacggca tccgccgccc accacccgaa ggtggtggga
    26161 cccgtcaggg gtaggagccg acgtaggccg gctgaccgga cctcgtcgca tccacctggt
    26221 cacccacgcc gttgacgacg tggcggatgg tgcccgcgtc gaggttgacc gtgagcaggt
    26281 gcgtcatctt cacgcccggg gtgttcggca cctcgaagcc gttctcggtg acgatgctcg
    26341 ggtcgttgcg gttgaacacg taggacccgc caccccacag ctggtgtgac ttcaccgtgg
    26401 cgccgacctt gtagccgggg tagcccaggg tgccgtcggc ctgggtccag tcggcctggc
    26461 tcggcgggtc gtagggcagc tcgttctggt agagcaccac ccgtccgcgc tcgccgttcc
    26521 agatggtgtt gtgctcctgg tagtgctcga cgaacaggcc ggtcgccgtg acgtcgtcac
    26581 cgttgacgat gagcccgttg cggccggtgt tggtcgccca gcgctgcgag tcgccctcgt
    26641 tggggccggt gaaaccctcg acgccgtggt cggcgcgcca cacccaggtg tggtcgacga
    26701 gcacgttgtc ggcgtcgatc tgcagcgaca cgtcggcctt gccgatgtgc ggtccaccga
    26761 cgcggaagta cacgtcgctg agcgtcaccg ggttggccag ggtcgaaccc tgagccgggt
    26821 tgcggggccg gtcggcgccg acctgcatga ggaccttcga gttcttcagc ccggcgtcga
    26881 tggtgacccc ggcgacgacg atgccggccc ggtcccacac cgtgatcggg gtggcgccgt
    26941 tcacggcggt cagcgtcgcg tggcccatgc cgagcacgac cgtgccgggg cgcttgatgt
    27001 cgatgctgcg gttgatgtcg tagacaccgg gggtgagcag caggttcttg ccacgagcga
    27061 gctggctgtt gatcgactgc accgagtcac cgggcttggc gatgtggaac tcggagaggt
    27121 ccagggtgcg accggccgtc aggccgtcgc cccacgtggt gccgcgggtc tcggccgcgg
    27181 cggcggggac ccgcaccttc caggcgccgc tgtcgtccat ccacagccac ggcttctcgc
    27241 ggctgagggg ggtggtctcc agcgtcgtgt agggctcggc cgggaagttc gcgtcgctcg
    27301 gggcaccctc gacgcccgcg aacacctggt tccacacgcc gttgctccag ccgccgacct
    27361 cgctgttgcg ggtcagccac tgctgctgcg agccgttgat gacgaaggcg gccttggagt
    27421 cggcgatgaa gccgccgctc gcgaactgcg ggcccgcgct gcagtagtcc atcagcgaga
    27481 ggttgccgcc ggtgacctcg acccgtcgca gggagaccgc ctggctgacg gcccagaagt
    27541 tcgccgtctg ccggcatccg tcctgaccgg cgccgttgac gttgatcgtg aggttggaca
    27601 gcgtgcgcca gaagttgttc agcgccacgc agtagctcgt gccaccgtcg cgaatgcacc
    27661 ggttgaacga ctcgaccttg ccgttgatga cgacgtcgcc cgggtcggcg cccaggcccg
    27721 cgacctcggt ggtgtatccc acacggatct gcagggggtc ctcggccgtg ccgtaggtgc
    27781 cgggcttgaa gaacaggctc cagcggttgg tgcccatctc gtcgtcgacc tgctcggcat
    27841 ggatgtcgtt gacgaccttc tcgatgtcgc tcacgggcat gctcgggtcg aacacgatga
    27901 cgttgtcacc gaacggggtc tgggtcgctg ctgcaggcag ggccgcgacg acggaggaca
    27961 gggacgccac ggatgccgtg gatgctgcgg gtgccgcact ggtggccgcc gcagcggtgg
    28021 cagtgctgac tgctgcggcg agcgtcaggg cgccgacggc gacgacggat gccgtggcgc
    28081 gcgttcgccg tcgggctccg tcgggagcca cgcgggtcgg gggtctggac tcttcatggg
    28141 caggcctctc ctcagcgcgc cccctcgccg atgagagagc gctctctcac cagagaacca
    28201 caccaggtcc acgctgacaa ccgttgcgag cggagcagtt ccagggttgg acaaggccgg
    28261 cggctcacca ggggcctcgg cgccaccggg cagaggcccg ggtgccgcaa gggccacacc
    28321 gccagatgca gccacggcgc ccacgtgcgt cggctgcccg cgtgcgtcgg ctgctcgcgt
    28381 gggccgcgcc ccctcgtcag ccccgctggg ccacgatctc gtcggcgtgg gcgaccaccc
    28441 aaccgaacag cggcagcagg tgcgcgacga gctcgtcgcc ccgcggtgtc aggctgtagt
    28501 cgacccgggg cgggatgacg ggctgtgcgt cgcggtgcac gaagccgtcg gccgcgagcg
    28561 tgcgcagcgt ctgggcgagc atcttctcgc tgatgccctc ggcccggcgg cgcagctcgc
    28621 tccaccgctg cggcccgtcg gcgagcgcga cgagcacgag gatgccccac ttgctcgtca
    28681 cgtggtcgag gacgaccctg ctgggacagg ctgccgggaa cacctcgtgg gcgaccggca
    28741 tccgtcgccc gtccacggcg cccgatccgc tgacctgctg ggttacctgc acgtgcgtac
    28801 cttacgtgaa agtgcgtact aaccatcggg aagtacttgc ctcctccgcc ggttggcccg
    28861 cacgaccacc cgcgcaccac ctgcgcacca cccgtgaaag aggaccaccc atgaccatcg
    28921 tcgtcaccgg agccaccggc cagctcggcc gcctcgtcgt cgagtccctc ctgcgccgcg
    28981 gggcctcccc caccgacgtc gtcgccaccg gccgcgacga ggcccgcctg gccgagctcg
    29041 ccacctcggg ggtgcgcacc gccgtcaccg actacgggga tgccgcgtcg ctggcagccg
    29101 cgttccaggg tgccgatcgc gtgctcctca tctcaggctc cgaggtgggg cagcgcctgc
    29161 cccagcacac ctccgtcgtc gaggcggcga aggctgccgg cgtcgggcag ctgctctaca
    29221 cgagcatctc ccacgccgac accgcgcaga tggcgctggc caccgagcac cgcgagaccg
    29281 agcgagtcat ccgcgagtcc ggcatcccgt tcaccttcct gcgcaacagc tggtacctcg
    29341 agaactacct ctcgcaggtg cccacctacc tcgagcacgg cgtcgtcggg gccactcggg
    29401 gcgggcggat cagcgccgcc acgcgcgccg actacgccga tgccgccgct gtcgcgctgc
    29461 tcgccgacga ctcggccggt caggtcctcg agctcggtgg gtcgtcgttc accctcgacg
    29521 agctcgccgc caccgtggcg tctgtctcgg ggcgggaggt gaccgccacc gaggtgtccg
    29581 ccgaggagct caccgcgatc ctcgtcgggg ccggcctgcc gcagggctac gcgcaggtgc
    29641 tcgcgagcgc cgacgagggc atcagccgcg gcgagctgga ggtgagcacc ggcgacctcg
    29701 agcggctcat cggtcgcccg ccgacgacgc tgcgcgaggc gatcgagcag accctcgcac
    29761 cctgacgccg cgaggtgtcg aacggacctt cgcggcctga cgcccgacgc cgacggcccg
    29821 gcaccacgtg gagggtgtgc cgggccgtcg ggggccgtac cgagggaggt cgctcaggcg
    29881 acgcgaccct tcggggcgtt cgcggccgtc agcgcggggt cgcgctcgac gacctcgccc
    29941 aggacgtcgt cgatcctcgc gaggagctcg ggctcgagct tcacgcccgc ggccttcacg
    30001 ttctcggcga cctgctcggg gcgcgaggcc cccacgagcg cggcagcgac gttctcgttc
    30061 tggagcaccc aggcgacggc gagctgggcc atcgtcagtc cggcctcgtc ggcgacgggc
    30121 ttgagggcct gcacgcgggt gaggacgtcg tcgttcatga agcgcttgat catgtcggca
    30181 ccgcccttct cgtcggcagc ccgcgaaccc tgcggcgggg cctgccccgg cacgtacttg
    30241 ccggtcagca caccctgcgc gatggggctc cacacgatct ggctgatgcc gagctcctcg
    30301 ctcgtgggca ccacctcacc ctcgatgacc cgccacagca tcgagtactg cggctggctc
    30361 gagatgagct ggacgttgag ctccttggcc agcgcgtgcc cggcccggat ctgctcggcg
    30421 gtccactcgc tgacgccgat gtagagggcc ttgccctggc gcacgacgtc ggcgaaggcc
    30481 gtcatcgtct cctcgagcgg ggtctcggtg tcgtatcggt gggcctggta gaggtcgacg
    30541 tagtcggtct gcaggcgggt cagcgagccg ttgatcgact ccatgatgtg cttgcgcgac
    30601 aacccggtgt cgttcttgcc gccggggccg gtgggccagt agaccttggt gaagatctcc
    30661 aggctctcgc gccgctcgcc cttgagggcc tcgccgagca cgctctcggc cttggtgttg
    30721 gcgtagacgt cggcggtgtc gaaggtgctg atgccgtggt cgagtgcagc gcgcacgcac
    30781 tgcgtggcga tgtcgttctc gacctgcgag ccgtgggtga gccagttgcc gtaggtgatc
    30841 tcggagatct tcaggccgct gttgccgagg tgacggaact tcatcaggcc ttgctgccct
    30901 tgagtcggtc gtacccgagg gtcacgatga tcgcgccgat gatggagccg atgatgccgc
    30961 tcggccgcag gttgaacccg tcacccgcga gcaggctggc aagcagccca ccgacgaacg
    31021 agcccgcgat gccggtgccg aaggcgagcg gccagttgat gccggacccg ccgcgtccga
    31081 ggatgagctg ggcggccgcg ccgacgagca tgccgaagag aatgattccg atgaagagca
    31141 tgggccgagc ctagccccgc cccgggagca tccgacccgc cagccccgag acgaccgacc
    31201 gggccccggc catgacggca ccgactcccc cggtcgtcgt gcccgagccc ccggcccacg
    31261 ccgggtcgcc gggcatcggg ccgagggggt cgcgctcctc cggcggcagg gtcaggtccg
    31321 gttcgcgcag cgcccgggcg aacgactcgt cgacgccgac gagctcgtag ccctcgggga
    31381 gcaggtcgta ggtccagtcg acgtcgtggc agaccatgtc gtggtgcagg ctctccacga
    31441 gcgcgggcac cgtcgatccg gggacgtcgg tgatggctcc ggcgagcacg gcgacgacgt
    31501 cggtcggcag tcccggcacc gtgacctggg ggcgggtgag cccggcgatg cgggcgtagc
    31561 ggtccagaag ctgcgtgtac ggcatccgtt ccgggccacc gacatcgtac gagcgcgatt
    31621 cgctctccac cgccagcgca cccgagagcg cctcgacgac gtcgacgacc gcgatcggtt
    31681 ggacgcggct gtccatccac gccggcaccg tgtgcaccgg catccgctcg ctgatctggc
    31741 ggaccacctc gaagctcgtc gacccggacc ccgtgacgat cgcggcgcgc agggtgagca
    31801 cgcggatgcc ggccgtcgca ccggtgagga tgcgctcgac ctccaggcgc gaggtgatgt
    31861 gttcggacag ctcgttctcg ggaacgtccg gaaccaggcc gctgaggtag acgagcagct
    31921 cgacccccgc tgcggcgacg gcccaggcca tgttctccgc cgagatccgg tcggtcgtcg
    31981 tgaagtcctc acccttgagg gcgtggatca ggtagtagac ggcgtccatg ccctcgacgg
    32041 ccgcctcgac ggtggcggag tccaggacgt ccatccgcac gagctcgacg tcgtcggccc
    32101 accacgccgt gcgggcccgc tcgaggttcg tcacggccgc cctcacgtgg tggccgtcgc
    32161 gcaggaggcg cgggatgagg cgactgccga tgtagccggt ggctccggtg accagaaccc
    32221 gccggggctc gggggtcgag gccgcggggt tcggggccat gtggttcgag gccgcggagt
    32281 ccggtgtgat cactggcaca acctaggccg atcgagggca agcttcggaa aacctttgac
    32341 accgggtggc tgccctggga cgtcgcatca gtcagaccga caacggcagc cgagcgcgca
    32401 cccgcccccc ggtgtcgccc gggcctacct cgatcgaccc gccgatctcg gccgcacgtt
    32461 cgcgcatgct cgacatcccg acgccggcct tccaccggcc ctgcctcttc gctccacggt
    32521 cgtggacctc gatggtgagt gccccggcat ccgccgacag ggcagcggtg acggtgtcgc
    32581 tgccgctgtg gcgggccgcg ttggtcaggg cctcgacgac gatgcggtag gcggcgacct
    32641 ccacggcggc ggtgaggggt ggcagcgtcg gcggggcctg gacggtgagc cgcagcggcg
    32701 tcccctcggg cgtgcgcagc gcgagcgcct gctggcggat ggcacggacc aggcccagtt
    32761 cgtcgagtgc cggggggcgc atggcgtaga cgagaccgcg gatctcgtcg atggccgtgc
    32821 tcgtctcggc ccgcagctgc gcgagcaggg cttcggcggc gtcggggtcg gtgcgcacgc
    32881 tgtttcgggc ggcgtcagcg gtgaaggcga tgccggacag gcgggggccg agcccgtcgt
    32941 gcaggtcgcg gcgcaggcgg cggcgctcct cctcgatcgc cgtgaccgac tgctcccgcg
    33001 actcctgcag ggctgcggcc agggcagccg agcgggcggt gagggcgagc aacggggcca
    33061 cgagggccag cacgttccgg tcgtcgccgg acagcgagac ctctccgggg cgcaggccca
    33121 cctcgagctc accgacgacg tccgccccga gggtgagcgg gagcgtcgtc gtgctcgtcg
    33181 ccgaccgccc cgacgaggcg acgctctccc ccgcaacccg cagcacggca tacggcaggt
    33241 cgagtgcctc gcgcacggcg cgcagggcga gcaccggatc cccgacgtcg ctgccggcga
    33301 cgtgggtggc ggcgtcgagc gggtcggcgc gcaggccgaa caacagctcg tcgacgacgc
    33361 cacgcagggc ggtgcgcagg gcgggcaggg gcaacgtggc gagggcaccg gcgacggcga
    33421 gctcggcgac cgcggcgggg cggcccagga tcacctcgag cagcgaggcc agcagcgtga
    33481 acagggcgat gtagccgatc agcaccgcgg tgacgacgac gagctggacg acgaccacgc
    33541 gcacgtcgac gacgtcgggc cgggtcgcgc cgacgagcat cgccgggccg acgagcgcag
    33601 ccgccagggt cgccaccacg gctccggcgg gcgtgggtgt gaggaacccg acgaccagac
    33661 cgatcagtgc ggccccggtg ccgacgaccg ccagccacgc cagtgccgac cgtgcccggc
    33721 ggggggctcg ctcgagacgc caccacgtgt gcacgacgag aaccgacccg agcacgacgc
    33781 cggtggcgtc gagcactggc tgcgactggg gctggagcgt cgcgaggatg ccggcgccca
    33841 ccaccgtggt gagggcgacg gcatccacgg ggtgcctcca ctgcaacgcg gggtaggccg
    33901 tgaccgcgag cggcatggcg agcgcccagc tggcgacgag gaggaaccgg gtgagccccg
    33961 gggcaccggt cgtctgggtg gtcatcgcct gcgtggtcat cgcctgggtg atcgtcgcgg
    34021 cgagcgtgcc cgcgccggcc accgtgaggg cgacggcgga gaccgtcgcc cggctgagca
    34081 gggccatcgc gagcccactc acggcccaga ccagcccgag cacggcgacc gccagggcga
    34141 cggacgtcga gctcactgcg cgctcccctg gcccaggccc tcctcgcggg ccctgatcat
    34201 cgcggccgcg cggtcggcga cctcgagctt ggcgaagatg ctcgtcaggt ggttgctcac
    34261 cgtcttgggt gagacgtaca gggcctgggc gatggccgcc gtgcgccgcc ccgaggcgag
    34321 caggtcgagc acctcccgtt cgcgggtcgt cagctccggg aagggttcgg gagcgggctg
    34381 ggcgtccggg ggctggtggg gcgcgccggc cgagaagtac gcgagcaccc ggctggcgac
    34441 gcccgggccg aagacgagct ggccggccac gaccccgcgc acggcgctga cgatctcgtc
    34501 ctggctggcg cccttgagca ggtagccgcg ggcgccagcc tgcatggccg tgaagacggt
    34561 ggcgtcgtcg tcgtgcatgg tcagcacgag caccgcggtg tccgggacgc tcgtgcggat
    34621 ccggcgggtg gcctcgatgc cgtcgccacc cggcatccgg acgtccatga ggacgacgtc
    34681 ggggcgcagc agctgcgcct cgcgcaccgc ggactcaccg tcgtcggcct cgcccacgac
    34741 ctcgaggccg tcgagggtgc cgatgagcgc gcgcagccct ccccgcacga cggggtggtc
    34801 gtcgacgacg acgatgcgca gcgcggtcac ctcaccatga tggcggcgag ggggtcgtcc
    34861 tgacccggga actcctgcct agccgcagcc ctcatcggcg ggactgggcg ggaaggtcgg
    34921 tgggaagcgg ccggggcgac ccgggaaggc gaacgggtac ggcatccctg tcggtcggga
    34981 acggtcgcgg cgcacgctcg aaccctcgcc aaccaccacc cgaggagtca ccatgaacac
    35041 catgaccacc gccccgtcac cgacccgggg caccacccgc tcgaaggtgg ggctcgccct
    35101 cgccgggctg ctcagcctca gcaacgtctt cagcgccttc ggcgacaccg cccccgaggg
    35161 cgaggtcggc ccgccggccg tcgtgctgtg ggccggcacg gtgctcggca tcgtcgggct
    35221 cgtcgccgtg gtcatcgcct ggcgcaccgg aaaccgcggg gcgctgcgtg tcgccgccgg
    35281 ggccatcatc atcacggcgc tcaccgcggt gccggcgttc ttcgtcgacg tgccagcctg
    35341 gctcaaggcc gcggcggcgg tcggggtgct gctcacgatc gcggccgtcg tgctgatgtt
    35401 ctccacgcag cgcggccacg ccaccgtgac ggactgaggt cgagtgccgt nnnnnnnnnn
    35461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaccgacggg tcccccgctg taccgacccg
    35581 aaggaagcca ccccatggcg atcatctcct gccccagttg cctctcggac aacgacatcc
    35641 acctgggcgc tgacgccgcc gacggcaccc gcaccttccg ctgcggcgag tgcgagcaca
    35701 cctggggtgc gagcgtggcc tccacgcggt ccgcctcgga ccgctccccc ttcgagatgg
    35761 ccaggggccg gttcgccacc gcatcgatgc tcagccccga gcgcgctgcc cgcgtcgtgc
    35821 ggctcaagcg ccagttcctc aagaacgagc cgctgccgac tcccgaggtc gacgagcacc
    35881 gggcccgctt ccagcacctg ttctccgccg aggtcctggc gacctgcgac ccgcaggacc
    35941 tcaaggactt cgccaccagc tcgatcggcg gtgagacggg caccgcgtcg gtctacaaca
    36001 gggcgtggaa caagctcggg gccgacgagg ccgccgagcg cacgcgcgag tcgatcgact
    36061 acctcctgcg cggaccggac gacgtccccc tcgaggaccg cctcacccac ctcatcgagg
    36121 aggacgtgga caccgcgatg cccggcttca aggagccgtt gctcaccaag gtgctgtgcg
    36181 tcgtgtaccc cgagcgcttc ctgccgctgt tctcctacac cgccgagggc accggcaagc
    36241 gcgagctcac cgagtccgtg ttcggcctcc acctgccggt caagcacgac gcgaccgcga
    36301 tgcagatcgg ccggctcgcg gtctggtcca acgacctgct gctcgagctc gtcggtgacg
    36361 ggttcaccgg cacgcaccac gccgcggcgt tcctgctgtg ggccaaggac cgcgcgggcg
    36421 cacgcccact cagctcggtg cgctgacccc ggggtctctg cggcgtccga atcggctcag
    36481 gagcagacgg catccgccgt ggcgacgagg ttgctgaggg cgccctggga gttgccgagc
    36541 tgtgcctggg ccgcagcggc atccagcccg acgagcgaca tgctggcggt cttcatgtcg
    36601 gcgaggatcg gcacggcgtc gatggggatg ccgttgagca acggggtcgt gtaggcggcc
    36661 tcgaagtcac cttgggtgac cttgccctgg tcggccaacc cgccgatctg ggtcgctccc
    36721 atgagggcgg ccgacatgcc ggcacactcc ggcgacccgg caccgtaggc cttgacggac
    36781 ggcaggtccg ccgtggatgg catgggcggg tccaaggggg tgctcgacgt gggatcggca
    36841 gcgggagtgg tcggcgtggc tggagccgcc gacgagggtg ctgcgtcagt ggcggatgcc
    36901 gccgggtcgg caggcgctgc gccgccacag ccagcgagcg cgatcagcac gggagggacg
    36961 agtcaggtca ggtgataccg agcctgcggg ccacagccgg gccacccatc tcgaggatga
    37021 cgtcgcgcgg gccgacgacg acgagcttgt cggtggcccg cgagagcccg acgtagagcc
    37081 gttcgcgggc tcgttcgcgg ggggtgctct cgttgacgca gagcacgacg accttgcgct
    37141 ccaggccctt gcagccgagc acgtggccgt agaagacgca gtccttgtcc cagaaggtgc
    37201 gccagtagcc gagctgcccc tcggactcct ggagcgagac ctgctcgggg tggcgggccc
    37261 cggtggtgag cagcgcgacg tcctcggggc gccagccgtc ctcgagcagc gggtcgatct
    37321 ggtcgtcggc ggcgtcgagg gcatcgtcgg cgctggcttc gacgtaggtg acctcgggcc
    37381 cttcgccgcc gcgcgcgtac atccgcatcg gcgcgagcgg caggaaggtc tcggtgatct
    37441 ggcgggtgtt gcgcaggttg tggtcgagca cgagcggcac cagcgggacc ggtggtcggc
    37501 cgaagcgcgc gaagacccgc tggttctcgt cgctgtagac gtagacgccg ccgttcagct
    37561 cgtcccgcag gcagcgcatg atcggcgtcc accagccgtc ggcgaagtcc tggccctcgt
    37621 cgacgacgac ggcatcgaac cgtttgccgt cgggcagttc cccggcccgc tccgccatct
    37681 gcgccggcag ccggttctcc cagaagtcgg tgtcgtcacg cccgccgaac tcgctcaccc
    37741 cgaggtagac ggcgaggtcc tcgaaggtgc cgacgaaggc gggtcggtgc cggcggtcga
    37801 cggtgtccat gtagcgcttg aaccagctgg ccagacccac cgagtaacag agcagggcga
    37861 cccgctgcgc gggctggccg tggcggccgt gggtgagctc cttggcttgg gtgagggcca
    37921 ggaccgtctt gccgctgccg gcgccgccgc gcacgtcgac ccggtgaatc agccgggtca
    37981 cgcccaggat cgtcgcctgc tcgatcgtca gccggtcggc gcgtgactcg cgttcgtcgg
    38041 cctcggcgag cagggtgcgc tggggcaggc tgcggccctt gaggatctcg acgatgaggt
    38101 cgcagtcgtc gtgggtgggc acgcggtggc cgctctcctg ccgggccgcg atgtcgtgca
    38161 ggcggccggc gaggtcgtgc tggtcacccc ggccgctgat catccagcgc ggacagtcgg
    38221 gggtggcgaa gtcatcggcg acgtcggtgt acggagcggc gatggcgtgg ccgaaccgca
    38281 ccctcgtgcg gctgctcgcc ttccagcgcg ggtcgcgctc gatgtacgcg cgcagggcgt
    38341 acttgccgtc gcggccctgg tcgacggggt gaaccacacg gcggcccttg ccgtggccga
    38401 cgacccactc gccgtcctcc atccgcaggt ccccgccctt gacctcgacg acgacgatgc
    38461 cgacgtccgg catgagcacg atgacgtcga gctcgtggtc cttcttgg
//
LOCUS       DAOSOC010000006        38065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1116_length_38065_cov_10.498606, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000006 DAOSOC010000000
VERSION     DAOSOC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 38065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38065
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1116_length_38065_cov_10.498606"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..1232)
                     /locus_tag="PL014_01030"
     CDS             complement(402..1232)
                     /locus_tag="PL014_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT55703.1"
                     /translation="MTTPVWTRYVAIGDSFTEGMSDPDPVTTGAYVGWADRLAVHLDA
                     VAEEAHVPFAYANLAVRGRKLDDVVGPQLDSALSLSPDLVSMVGGGNDLLRPSVDLDS
                     LASRIEDAVVRLRRAGADVLLATPADPRDAGLFKALRPRHAVHSANIFTIAQRHECHV
                     LNLWGIAALRDWRMWGEDRIHLSSEGHRRVALAALTALGHGTDVAEWATPLAPATRGA
                     RRDEFREHAQWARTHAAPWVQRRLRGESSGDRLAAKRPTLTPLRDPDLEPAHGSLPEG
                     "
     gene            complement(1229..1918)
                     /locus_tag="PL014_01035"
     CDS             complement(1229..1918)
                     /locus_tag="PL014_01035"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT55704.1"
                     /translation="MTSTDAARPLRDLVHPSWARALAPVEPVVARLGDFLRAEVADGR
                     GYLPSGPNVLRAFQQPLDAVRVLVVGQDPYPTPGHAVGLSFSVAPDTRPVPRSLANIF
                     TELEADLGLTRPATGDLTPWAEQGVLLLNRVLTVAPGAPGSHRGRGWEEVTACAISAL
                     VERGGPLVAILWGRDARGLAPHLPGVALIESAHPSPLSARNGFFGSRPFSRTNDLLAR
                     QGADPVNWSLP"
     gene            complement(2080..2451)
                     /locus_tag="PL014_01040"
     CDS             complement(2080..2451)
                     /locus_tag="PL014_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT55705.1"
                     /translation="MSTTATRPATSAVATATATATASCCGIGVDGGLTREAAETSAAL
                     LKAVADPVRLQLLSVIRATADGEACVCDLTPVVGLSQPTVSHHLKVLVEAGLLEREKR
                     GTWAWFRLVPARLDDVTAIFR"
     gene            2547..3107
                     /locus_tag="PL014_01045"
     CDS             2547..3107
                     /locus_tag="PL014_01045"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HOT55706.1"
                     /translation="MNTPDAVDPVAAAGGASRVQLALNVSDLEASIAFYSAMFGVEPH
                     KRRPGYANFAIAEPPLKLVLIETSEATRGTGTAGALNHLGVEVPSTDDVTAARTRFTD
                     AGLATFDENDTTCCYALQDKVWVHDPAGAPWEVYTVKDEDPVDARPATASLEILGGPT
                     AGACCSPSADATPVDSAPEKAQACCS"
     gene            3104..4186
                     /gene="arsB"
                     /locus_tag="PL014_01050"
     CDS             3104..4186
                     /gene="arsB"
                     /locus_tag="PL014_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOT55707.1"
                     /translation="MTTASPARDTASAGPDQPRHLSTLDRFLPVWIGLAMLAGLVLGR
                     LVPGLGEALSAVEVEGVSLPIAIGLLVMMYPVLAKVRYDRLDTVTGDRRMLGASLVLN
                     WLVGPALMFTLAWLLLPDLPEYRTGLIIVGLARCIAMVIIWNDLACGDREAAAVLVAF
                     NSVFQVLMFGVLGWFYLSVLPGWLGLPQADLDVSPWQIAGSVLVFLGLPLLAGYLSRT
                     VGEKRWGRTAYEASFLPRVGPWALYGLLFTIVILFALQGDQITTSPLDVARIALPLLA
                     YFALMWGGGFALGAALGMTYERTTTLAFTAAGNNFELAIAVAIATFGVTSGQALAGVV
                     GPLIEVPVLVGLVYVSLALRKRFRHV"
     gene            4179..4604
                     /locus_tag="PL014_01055"
     CDS             4179..4604
                     /locus_tag="PL014_01055"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOT55708.1"
                     /translation="MSDLPTTRPSVLFVCIHNAGRSQMAAAWVQHLSGGAVEVRSAGS
                     APAGSVNPAAVEAMLEEGIDMSAEVPKILTTDAVRESDVVITMGCGDTCPIFPGKRYE
                     DWALEDPAGQGVDAVRPIRDEIRVRVLGLLESLGVQPVA"
     gene            4634..5290
                     /locus_tag="PL014_01060"
     CDS             4634..5290
                     /locus_tag="PL014_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT55709.1"
                     /translation="MRPRFHTVLFDLDGTLADTIPLIVASYQHAFRTVLGEEIDEARA
                     RAWIGRPLLAALLEESPEHGHDLDQTYREWNLANTARLIRRYEGVPEMLEALASAGVR
                     MAVATSKRRQTARLALEGVGIDHLIEVAAALEDTTRHKPEPDPLLHAAASLGAQPCDC
                     VYVGDATVDVLAARAAGMAAIAVSWGAADREALAATGPDALVDTVADLTAYLLGHDEG
                     "
     gene            5364..6665
                     /locus_tag="PL014_01065"
     CDS             5364..6665
                     /locus_tag="PL014_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT55710.1"
                     /translation="MAYAEERVRMAPPLDRPVTEAELRSRVGETITPGGLGGVEAMRL
                     FAQDLAPTCLSTDHPRYLSFIPCAPSDAAVMFDLVVGASSIYAGSWLEGSGAVYAENQ
                     ALRWIADLVGMPSGAGGVFTPGGTIGNLSALVTARHTARASAKPGDRPYRVAATAGAH
                     SSIASACDVMDAQFVGVPADDAWRLTGENLRAVLEENGPETFFAVVATCGTTNFGVID
                     DLASVAEVCREYGIWFHVDGAYGGAGLAAPSVRGLYAGIEHCDSFIVDPHKWLFAPFD
                     CCALLYREPSLARAAHTQKAGYLDVLTDAPDWNPTDYSVGLTRRARGLPFWFSLVVNG
                     SDAYEAAIEHTLDVARFAEAEVLRRPDLELVRERDLSVVVFRRLGWEPADYAAWSDRM
                     LAEEFAFVVPTSHDGETLARFAIVNPETSEDDITAILDTMD"
     gene            6866..7159
                     /locus_tag="PL014_01070"
     CDS             6866..7159
                     /locus_tag="PL014_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HOT55711.1"
                     /translation="MSAASEQLSTAAPAAGLTERDREIIAFERQWWKYAGSKETAIKE
                     LFDMSSTRYYQVLNALIDHPGALEHDPMLIKRLRRLRASRQRARSARRLGMEP"
     assembly_gap    7253..7352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7353..7951)
                     /locus_tag="PL014_01075"
     CDS             complement(<7353..7951)
                     /locus_tag="PL014_01075"
                     /inference="COORDINATES: protein motif:HMM:NF023257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HOT55712.1"
                     /translation="MVDTNSQALRYLAEAYPDGIPEEDVAPLLALLRQRIGSTRTLSV
                     ALALVAGGALSADHAASGSAPKAPLPQSDLRRVAARLVLGGWPLGNPPGDDLDDEDDT
                     EPGSYLGRIVAWLREGYPTGVPEHDYVPLFALLERRLTRGEVKKVAKALRRASIAPAS
                     PDDIAAAIADLTHSEATPDDLRRVRDRLAAKGWPVDFPDP"
     gene            complement(7975..8286)
                     /locus_tag="PL014_01080"
     CDS             complement(7975..8286)
                     /locus_tag="PL014_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55713.1"
                     /translation="MRSIELALQGAASVLFAGLIFGAGLPVVYALAMRALVTGSTETE
                     DASGHTTFHPTVLGRAVAGVLLAVIVASVALGITMIAASGFGKTVSFSGDGILPTIVD
                     K"
     gene            complement(8303..9568)
                     /locus_tag="PL014_01085"
     CDS             complement(8303..9568)
                     /locus_tag="PL014_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOT55714.1"
                     /translation="MDAATLTLVLVIVTALAFDFTNGFHDTANAMAASISTGALKPKA
                     AVALAAILNLVGAFLSVEVALTVSNAVINIQDKSGAPKEEFLSGGGQGLLLVVLCGLV
                     GGIIWNLFTWLLGLPSSSSHALLGGLTGAAAAAYGLSGVKWTGDGTKLDGVVGKVLLP
                     SFLSPVIALIVAFIGCWLVHRITNSIVHKLGEGTFRYGQIGSASLVSLAHGTNDAQKT
                     MGVMTLALLASGHWTDTTSVPWWVKVSAALSIAAGTYLGGWRIIRTLGKGLVDITPRQ
                     GMASDGATAATLLAASHLGFALSTTHVATGSVLGGGLGRGTNVRWNTAGRMVTAWATT
                     FPLAALVGALTWWLGNSIGGALGAVIVFIVLGLASAYMFWRSKQDNVGAHNVNDEWDT
                     ADDVLDKFPPAPETPEDPAGTTTHRTKSH"
     gene            9683..10147
                     /locus_tag="PL014_01090"
     CDS             9683..10147
                     /locus_tag="PL014_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55715.1"
                     /translation="MSDLDWSTVDGLAAIREHLAARIEGWRQPVAYAVALSPASSSPE
                     WDFAHVNTPDGRHGLPAVVLATILGHDGSTASLPVSAHQLAAAVESLAPAQACTAVDH
                     PNLAAWRAALADLEGNPAREAVAVFVADLHDPVTSEADGSMRATFEGHAPAL"
     gene            10260..10889
                     /locus_tag="PL014_01095"
     CDS             10260..10889
                     /locus_tag="PL014_01095"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOT55716.1"
                     /translation="MLDTLTALVEAGAAATWVLPVVLLVAVFDALVPPVPSEGIVVAL
                     AAVAVAGDGPNLMALAVAAGVGAFLGDSLTYVVGRRFGPRRLHRITRPAARRALERAS
                     DTLDRRGAVVILTARYVPLGRVAVNLTAGATGFPPRRFFGLAAVAATTWAAWSVGVGA
                     LAGHWLEGNPLLGSVLGVVAALLLGLVIDRVARRASGWGRMPSAVVART"
     gene            11127..12752
                     /gene="groL"
                     /locus_tag="PL014_01100"
     CDS             11127..12752
                     /gene="groL"
                     /locus_tag="PL014_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147142.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT55717.1"
                     /translation="MAKIIAFDEEARRGLERGMNVLADAVRVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELDDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LRNVAAGANPMALKRGIEKAVAAVSDELLAAAKDVETKEQIAATASISAADPLIGEMI
                     AEAMDKVGKEGVITVEESNTFGLELELTEGMRFDKGYISHYFVTDTERMEAVLEDPYV
                     LIANSKIGSIKDLLPLLEKVMQSGKPLLIISEDVEGEALSTLVVNKIRGTFKSVAVKA
                     PGFGDRRKAMLGDIAILTGGQVISEEVGLKLDTAELDLLGRARKVVVTKDETTIVEGA
                     GDEEQIAGRVNQIRAEIEKSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVAGGGVALIQAGKAAFAKLSLEGEEATGANIVRVAIEAPLK
                     QIAINAGLEGGVVAEKVSNLDSGWGLNAATGEYVDLIAAGIIDPAKVTRSALMNAASI
                     AALFLTTEAVIADKPEKNPPAMPGGDDMGGMGF"
     gene            complement(12822..13439)
                     /locus_tag="PL014_01105"
     CDS             complement(12822..13439)
                     /locus_tag="PL014_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55718.1"
                     /translation="MTTPNHPAVDEWLARLDTEASALPAERRAELRTSIDEYLADAVG
                     TTPRSEAAVRQVLADLGDPADLVAEASGQAVAPEIEYLAPDPFGAPVTGPPWLEVVTI
                     TLLAASVVLALVPATSAFAPLPWLIGCVLVLLSRRWGAADRALAVVAFGVLGVPLLLL
                     GRDELPTTSSLVVAVALAALWLGTAVRLLLRARTPVDQGRGLRMT"
     gene            13552..15273
                     /locus_tag="PL014_01110"
     CDS             13552..15273
                     /locus_tag="PL014_01110"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOT55719.1"
                     /translation="MTTATATDPLVLGPLLRYVDESSATIWVRTADPALVTVERAGHV
                     WSAPTFAVHGSHFALVVLDGLEPGSDDAYEVRLDGTPVWPREGMPPSRVRTLDPTREP
                     RLAFGTCRTTGSHDEEGNRAHGVDALRSFAIALRDDPATDWPDLLLLLGDQVYADTTP
                     HPELEEFMAARRDLSLPPGDEIKDFVEYAELYRLAWSDDVVRWVLSTVPSAMMFDDHD
                     IRDDWNTSWSWRREIRATTWWQERIVSGLGAYWVHQHIGNLSPAELADEEVWRHVSAH
                     AASGEPGELDLSDVLDAVAARADEEPSTYRWSYARALGDCALVVVDSRAARELRPDGR
                     SMLDADEMRWLDAVMRGGVRHLFIGTSLPFLLPPGLHDFEAMNEAMAQGSHGRRVARG
                     AERVRRRIDLEHWAAFNEGFDEVFEMVMAVARGERGAAPQTVTFLSGDVHNSYLAQVS
                     DPERLGASSRIVQAVCSPIRNPMPRAVRVVMSLFARSLVRPMRFIAARSRRVPDPAYP
                     WTVTDGPWFDNNLALVTVGERSLDLTWVTGVVVDDVDRPALQTVYAAHITPLASSPRL
                     VEGLSKV"
     gene            15389..16165
                     /locus_tag="PL014_01115"
     CDS             15389..16165
                     /locus_tag="PL014_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55720.1"
                     /translation="MTLAETTARETTPRLAAARVEGLSKVYGSGETRVVALDDVTLDL
                     YAGEFTAIMGKSGSGKSTLMHCAAGLDEASAGHVFVGDVDLTTLKERALTELRRDRIG
                     FVFQAFNLVPTLTARENIVLPAAIAGREPDAAWFDEVVDTVGLRDRLGHRPSEMSGGQ
                     QQRVACARALVGRPDIVFADEPTGNLDSTSGAEVLGFLRRVVDTHGQTVVMVTHDPGA
                     ASWTDRVVFLADGKVVDELREPTPELVLEKMAALGRPVGA"
     gene            16169..18724
                     /locus_tag="PL014_01120"
     CDS             16169..18724
                     /locus_tag="PL014_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT55721.1"
                     /translation="MLKATWRNVFAHKLRLFLSAFAVILGVAFVAGSYIFTDTLDRAF
                     TGLTSGAVGDVIVQPGSSDDEEASGPGALGTRTVPASLVDELGAVPGAARADGRVSNF
                     GTFVVGKDGKLIGGQGPPGIAVNRTEGPAANGIPSATLESGRWPEAPDEVVLDPSTAR
                     KAGYLLGERIPLVTTSEVPRIEVTYVGTASFGASALVGASVVMLETSKAQEVYLGGED
                     AFSAVWVTAAPGTSQEQLLASVKEVLPEGFRAATGDATSERASTRIDEALSFVTTFLL
                     VFAGVALTVGAFLIVNTFSILVAQRSRELALLRAIGASRRQVARSVLAEAGVVGLVGS
                     AVGIAMGFVLAVGIKLLFSRIGLDLSANELVLRPRTVVVALVVGILVTLAAAYLPARR
                     AGRVPPVAAMRDDVALAESGLRWRLVVGAALLAAGIAAMAAGLAGLGSQPTYVLGAGA
                     FGVLVGTALLSPVLGRPVLAALGWFYRRSFGAVGLMAEQNARRNPRRTAATASALMIG
                     VALVTMMSVLGASAKASLDQSLSEDIVADFVVSNAVGQAFSSTVADEIEQVDGVAAVA
                     RVRSAVLQVDGDRDFASAVDPAAVDAVARPEIVTGTLADLDATSVAISSELAADRGWA
                     VGSTVKIGYAGATTDATVVATYVEGAVLQSDVTMSLEGFDAIGVPPTDRTVYVVAAPG
                     VDRAVLGAALKDVVVDLPTVTVNDQETFAEEQRAPIDQLLFIVYALLGLAVVIAVLGI
                     VNTLALSVIERVREIGLLRAVGLSRTQLRTMLRLESVAIALLGAVLGIVLGLAFALAL
                     QRSLIDDGIDVLAIPVGQLALFVAVAAVVGVLAALWPGRRAAKLDILRAIASD"
     assembly_gap    18873..18972
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18973..19239)
                     /locus_tag="PL014_01125"
     CDS             complement(<18973..19239)
                     /locus_tag="PL014_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HOT55722.1"
                     /translation="MAAQFTVDTERIAGAAGDIARISAEIEGQVATMMARLNGLQDAW
                     TGTASAQFQGVVSQWRGTQQQVRTSLDSIGQVLGAAGTQYADAEA"
     gene            19593..20369
                     /locus_tag="PL014_01130"
     CDS             19593..20369
                     /locus_tag="PL014_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HOT55723.1"
                     /translation="MAILIDPPRPEAHGRRWSHLISDTSTEELHAFARAAGIPRRAFE
                     GDHYDIPEERYAAVVAAGAEPTPARDLLRALQRSGLRLQKRRGDTGVAKITGIRFVDG
                     TSADVDLIASPREADVGRVFAAMAFVRDDTGDFALVHSVRRSEWGAPGGWREPGESVR
                     ENAVREVREETGLHVPPRDLTPIGYERFHHRSSGGLWREGQDLLQVYEVSVPGIRPAL
                     TPELDDTSHHQWVTWDELEALCSRQFWWALAEATLSPPAG"
     gene            complement(20350..21063)
                     /locus_tag="PL014_01135"
     CDS             complement(20350..21063)
                     /locus_tag="PL014_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55724.1"
                     /translation="MRALQRRWVADCAVAAPLAAREVVDDVGHDLLTRWSEEHRRYHG
                     LTHLSEVLAALDRLCATRAVSAEDATVAALAAWFHDAVYSVTPATPDRPGTSGAAAAA
                     RTAAVSNEAASADLAEHHLSALGVAAATRQRVTASVLDTEHHDLGSTAGEDPARVLLH
                     DADLWILAAPVVRFDEYCAQVRAEYAHVPAAVYARERSAVLRPFLARDNVYRTEYARS
                     TWGSAARENLARELTRLAG"
     gene            complement(21096..22985)
                     /locus_tag="PL014_01140"
     CDS             complement(21096..22985)
                     /locus_tag="PL014_01140"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HOT55725.1"
                     /translation="MSTRAQVQQLEAEFADAMTRMYTVGNGLARLRSQLEREEGYGIP
                     DAAPPAPVPPPVARPAGPPAVPAAPPITAQVPAPPATLGPWQTPTPSVPWYRREGAVT
                     RVLAIAGAVITMAGVAMFLVLAAQRGWFGPEARVAAGAVLAVILIGLGVRSGRADLRG
                     RDTVEGAPAAAVGAPSGVGAAPVALVATGVATAYLVVVAVTTGYGWVPTAPGLALAGA
                     VALGGLHLARSWRSELLAVLVVLGAAVLAPVVAQGGGWVVTGYLAILCLAGWWASGDR
                     TTPVLTLARVLPLTLALLVSSVAGGGTVDDVGNLAIAGIALAAALFTSTLSVRRDPGD
                     LAASIAVALLAIGLFAATTTFDNPARTLSLATASAVLLLTATTLGRGPVGPVAGHLVV
                     TTGAAGTAFAVLAVVAGAPTRFITTGLLLLAIVGLAVAGITRSRVNLVLAAGVTLLGV
                     VGWLQYPLTVAASARTLRGDMAAALLDSIVLAGVLAMVVWAMGQQRGLGRELRVMVRI
                     AVWVLGLAASATALAAAGTLVGARLGDPTLGFTIGQAVATITWMLGAAWLLLHGLERS
                     PDADLTLRTGLLLAAVSVAKLFLYDLSSLSGIVRSVAFIAVGLLLLAMGSRYAKAYER
                     SRPPS"
     gene            complement(23113..24441)
                     /locus_tag="PL014_01145"
     CDS             complement(23113..24441)
                     /locus_tag="PL014_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT55726.1"
                     /translation="MTDQNPTPQWYAPRGGTTTDPRPVGTFGQPTEPVPTWPPAPPAP
                     PGNSMMPAMTTPPAPRRGRRLAELTAVAVITGLIASGSTLAITGAYDGTDAATTTGTS
                     QSLGRDADAAPVVQADGSAPNWTATASAVSPSVVAITATSAQGGGQGSGVIIDSSGHV
                     LTNNHVVAGAQELTVTTSDGRTFAAEIRGTDPSTDLAVITITGNPSNLTPIAVGDSDA
                     IAVGDPVMAVGNPLGLAGTVTTGIVSALNRPVTTQGESSGQDQFGQAQSQAEPVVTNA
                     IQTSAAINPGNSGGALVNAGGQLIGINSSIASLGSSSGQSGNIGIGFAIPVNEATSIA
                     EQLIAKGTAVHAYLGVTPQDGSASDGSATRTGAEITSVGADTPASKAGLQVGDVVTAV
                     GGERVESALSLVAHIRERSAGDEVTLTVLRDGKTIDVKTTLAAKPTTTSP"
     gene            complement(24512..26065)
                     /locus_tag="PL014_01150"
     CDS             complement(24512..26065)
                     /locus_tag="PL014_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT55727.1"
                     /translation="MTSASPATTPPASDPPASHPPASTPPAAKDDPVARVVRHLEAVP
                     LRRRLVAIVGTLVGAALILTSLATAYLMRSDLLDRVDSELRSVARPVANQVFDDLRST
                     GSALPTGYAFHLQGARGAITRLPTGETARPVLPVLTVDDPRVTSGEPFTVPSEGGRPQ
                     WRFIAGRVVGEDSTFAVGVPLDTVNDTVTQLVITTGLISTIVLLASLAMARYAVRRAF
                     RPLTRIEDTAAAIAAGDLTQRIPVRQADDEVTSLSRSLNTMLARIESSFAVREASEER
                     MRQFVADASHELRTPLATVRGYAELYRQGAVRDPEAVGGAMERIEAESERMSGLVEDL
                     LMLARIDDAPEVELAPVDLTVLAADAVADARVRAPERRISLLGLGGPVAPTVVVGSEP
                     KLRQVVTNLVANALRHTPAGTPVEVAVGLDAHGATLEVRDHGPGVPPEVATKVFERFY
                     RADPSRGRTAGGGSGLGLAIVAAIVARHQGQVGVARTPGGGATFVVRLPQRPSPAERR
                     PVAQRTPSN"
     gene            complement(26068..26781)
                     /locus_tag="PL014_01155"
     CDS             complement(26068..26781)
                     /locus_tag="PL014_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT55728.1"
                     /translation="MTSTPTPEARLLVVEDEPNIRELLATSLRYAGFDVATAASGAEA
                     LRVADAHDPDLCVLDVMLPDMDGFTVTRRLREQGRQLPIVFVTARDSVDDKIKGLTVG
                     GDDYVTKPFSLEEVVARIRAVLRRTRGDAEQDSLVLTFEDLELDEDSHEVRRAGRVIE
                     TSPTEFKLLRYLMLNPNRVLSKSQILDHVWDYDFRGEAGIVESYISYLRRKIDADGLP
                     PLIHTKRGVGYVLRLPPQA"
     assembly_gap    26833..26932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26941..27879
                     /locus_tag="PL014_01160"
     CDS             26941..27879
                     /locus_tag="PL014_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOT55729.1"
                     /translation="MRLPLPQVEPDATLPESVTIYEVGPRDGLQNEAAVVPAVVKAEF
                     VRRLASAGLGTIETTSFVDPRWVPQLADAEEVLGLLDEQGRGAQRPVLVPNERGLDRA
                     LAAGVGAVAVFGSATEMFARKNLNRSVEESLAMFAPVVERARAEGLWVRGYVSMCFGD
                     PWEGPVPVEQVVEVCRRLMDLGCDQLSLGDTIGVGTVGHVHRLLAGLDAVGITVDRVG
                     VHFHDTYGQALSNTLAALREGVTVVDAAAGGLGGCPYAKSATGNLATEDLVWALDGAG
                     VHTGVDLDALVATSAWMAGHLGRPSPSRVVRALTGA"
     gene            27983..28717
                     /locus_tag="PL014_01165"
     CDS             27983..28717
                     /locus_tag="PL014_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55730.1"
                     /translation="MSDPAAPAPQPGSAPVSPQAPAPDPGSHGWFARAASTTGGQVAL
                     VLGAVFTTLLLVTGVGLATAAVVGGVLYAHGDDGRMPVSDRGPGQGKGLALGHDKQRG
                     MGRGDDQGQGRGEAEGAPGRGNGNGKGGPGAGQGQGNGRGAPGMGNGMGMGRQGLGAP
                     DVLHGEFTTNVTGTPTVMVVQSGQVTAYTAGKSLTVRSSDGFEATYALDEAVASMRGA
                     ADLAAGAQVQVIAAKEGMKVTRLSVE"
     gene            complement(28695..29993)
                     /locus_tag="PL014_01170"
     CDS             complement(28695..29993)
                     /locus_tag="PL014_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT55731.1"
                     /translation="MEPNASRLTLRGFWESLPTPGRWLLSTTAISTLGRGMTLPFTII
                     YVHEVRGIELDVAGLLMSLIAGVALVVTGPVGALIDRLGSRVMLIWGNLAQLVGAVIL
                     AFATSVPAFVAAFTLIGISFGIGWPGFNALTSSIVSGPLRTQFFGINFALVNLGIGIG
                     GVLSGFLTDVDRPATFTAIFLADAACMLIPIALLLGPLRRTGGPPQASAPSGPQDTAS
                     AVPGVSYLTILRNPAVVWITALTFLSSFVGYGQMEAGFPAFAREVSEVSTRTIGLAFA
                     ANTAVIVGMQFLVLRIIDGRRRTRVFLGLVALWAAAWLILGATGLVAGTLAAAAGVIV
                     FHIFFGLGETMLQPTVPAIVNDLASDRDRGRFNAVSSGAFQGGAITAPIVAGVLLDRE
                     LAGVFIGLLIACLAVVALLALALERRITPEVNGLTPPTGE"
     gene            30190..30588
                     /locus_tag="PL014_01175"
     CDS             30190..30588
                     /locus_tag="PL014_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT55732.1"
                     /translation="MPTGKVKFYDADKGFGFVAGDSGEGDVFVPSSALPQGVTGLKGG
                     QRIEFSIAEGRRGAQALSVRLLDPTPSVADNRRVAGRKPADEMVVIVEDVIKLLDSVS
                     EGLRRGHYPDKAHGNKIAQVLHAVAGDLEL"
     gene            30609..31337
                     /locus_tag="PL014_01180"
     CDS             30609..31337
                     /locus_tag="PL014_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HOT55733.1"
                     /translation="MKTDATLAAAVETAREAAVSIAEADAVGAHLGMEMVQDRVATHY
                     FECAALAYRGWRWAITVARVPRSKTVTVSETNLVPGEESLLSPQWLPYAERLAPGDVG
                     PGDVTPLIADDPMLVAGFEATGDDDVDQVAIWELGLGRARVLSAEGREAAARRWYEGE
                     SGPTAPIAVKAPAPCSSCGFFVPMAGALRSVFGVCASEWSPSDGRVVSLDHGCGAHSE
                     VDMDAPEPERVTAPILDDFELDPA"
     gene            complement(31349..31615)
                     /locus_tag="PL014_01185"
     CDS             complement(31349..31615)
                     /locus_tag="PL014_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HOT55734.1"
                     /translation="MTPTDPAPEQRATPLGQSAPVDPLTVPTRTIVLVGTAIWAVALV
                     VTLLVPDLHTGERSWWPWACVSGLGLGAFAWWYVNRTQGVDDPT"
     gene            31839..32330
                     /locus_tag="PL014_01190"
     CDS             31839..32330
                     /locus_tag="PL014_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT55735.1"
                     /translation="MSPITAPVAPSSTQATRPPVPRPMTRAQRDRLTSDLRMACMRIA
                     RRVRLESDALVAPHQFSVLVRLEERPSTPKDLATIEKVSAPSMTRTVNALVDLGLVAR
                     ADDPTDGRQVILSLTPAGRETIKETRRRRHQWFASQLETLTDDERALLVQATALLERV
                     AAE"
     gene            32327..33631
                     /locus_tag="PL014_01195"
     CDS             32327..33631
                     /locus_tag="PL014_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT55736.1"
                     /translation="MSPTFTSFEVRNYRIWFAGALVSNVGTWMGRVGQDWLVLTVLTA
                     GSATALGVVTGLQFLPFLLLAPWAGLIADRFPKRRTLLLTQTMLAVSSLLLGVLAVTG
                     LAQLWHVYVIALLQGVTTAIDNPARQAFVSEMVPNDKLANAVSLNSASFNLGRLIGPG
                     VAGLVIAAWGTGQALLLNTVTFVFVIAALLLLRANELKPAPLAKARGGIREGFSYVRN
                     RGDLQLVMLLVFVLGTFGMNFQITMALMATKVFDKGAGEFGLLGSIMAIGSLSAALFS
                     ARRKVPRLRVLLISLAAFTVAATVAAMAPTYWTFALALVPVGFSALTALTTANAMVQT
                     RVAPEMRGRVMALYMAIFMGGTPAGAPLIGWISETFGPRWTIGIGAVAMGITLVGVSV
                     WLARSQNVRVSYESQRRPRVRVAVTSLPTPATAIAPVPEPAR"
     gene            complement(33815..34942)
                     /gene="serC"
                     /locus_tag="PL014_01200"
     CDS             complement(33815..34942)
                     /gene="serC"
                     /locus_tag="PL014_01200"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOT55737.1"
                     /translation="MSDAPITIPADLLPADGRFGSGPSKVRPAQIEALSAIATTVMGT
                     SHRQKPVRDIVAQVRSGLADFFSLPEGYEVVLGNGGATAFWDVAAFGLVRERAQHLTF
                     GEFSSKFATVTKNAPFLGEPTVISSEPGTRPQPRAEAGVDVYAWAHNETSTGVMAPVH
                     RVEGADADALVLIDATSGAGGLPVDVAQSDVYYFAPQKSFGSDGGLWIALFSPAALAR
                     VEEITASGRWVPDFFSLPTAISNSRLEQTYNTPALATLAMLANQVQWLNGQGGLEWAS
                     ARTKDSSGRLYDWADASSYATPYVTDPANRSQVVATIDFDDAIDASAIAKTLRANGVV
                     DVEPYRKLGRNQLRVACFPAVEPDDVSRLIGCIEHVVEHLA"
     gene            complement(35149..36267)
                     /locus_tag="PL014_01205"
     CDS             complement(35149..36267)
                     /locus_tag="PL014_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HOT55738.1"
                     /translation="MSTAATTDPDFRPGLEGVIAFESEIAEPDKEGGALRYRGVDLKD
                     LVGRVSFGQVWGLLVDNEFDPGLPPAEPFPLPVHSGDIRVDVQSALAQLAPVWGFKPL
                     LDIDDAEVRDNLARASVMAISFIGQSAHGQSSPMVPQSEVDKGRTIAERFMIRWRGEP
                     DPKHVEAIDAYFISAAEHGMNASTFTARVIASTGADAAACLSGAIGALSGPLHGGAPS
                     RAQHMIEGVEQMGDARRYVKEVLDRKERLMGFGHRVYRAYDPRAAVLRDTCQRLGAPR
                     YEVALELEKAAIAELAERYPERRMETNVDYWAAVLLDFADVPGTMMTPLFAAARTAGW
                     SAHVLEQKRTGRLIRPSARYVGPGPRPLEDVAGFDPER"
     gene            complement(36329..>36658)
                     /locus_tag="PL014_01210"
     CDS             complement(36329..>36658)
                     /locus_tag="PL014_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HOT55739.1"
                     /translation="AAEKATERAATPETPARLTRNQLRSDAKLVALLRRTVEGAADDA
                     GWARVSTVGQRISNQSSFDPRNYGYAKLSKLLEATDLFELRDVGAPAMAVRDRRMSTH
                     GTAGGAN"
     assembly_gap    36659..36758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36759..37414)
                     /locus_tag="PL014_01215"
     CDS             complement(<36759..37414)
                     /locus_tag="PL014_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOT55740.1"
                     /translation="MDKPDQIALLIDADNATAARIDVILDELSRHGETNIRRAYGNWT
                     KTGLKSWQEVLHDNAIRPVQQFDPSKGKNASDIALAVDAVEILHTQRPDWFAIASSDA
                     DFTPLVMHLREHGAKVYGFGDAKTPQPFQSACTRFLVLNRLAATSPLPDAVAPVAPES
                     APAAPPRRRASARAATVEGATTNAADTASALDTASAAPPAKKTTARKKAATLAAAEKA
                     A"
     gene            37470..>38065
                     /locus_tag="PL014_01220"
     CDS             37470..>38065
                     /locus_tag="PL014_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT55741.1"
                     /translation="MLRSHLPDLTPLRENAAYRRLYVGFTLSNVGSQMAVVAIGLQVY
                     DITGSTASVGLVGLFALVPLVVMGLYGGSLSDHHDRRTVALAANIVAWLTSIACAAQA
                     WLGNENVWVLYAIVAVWNGAFGVSSPARSSIYPRILPREQLPAANALGVFAMSTAMMV
                     GPLLAGLLVDWGGFRTAYSVDALITMAALWGLWRLQSLP"
BASE COUNT         5217 a        13003 c        13944 g         5501 t
ORIGIN      
        1 gggtgctgga caggcgctgc gcgtcgatca tgcggcgcac ggtgtcgttt gtttttttca
       61 agcagaaggc ggcatacgag atccgaatct gcgacatgga gcggactcta cctccgcatc
      121 tgcggaactg cgagggtagc ggcaagtgat gggacgcctg gtagggggcg aggcgtaccc
      181 aaatcgaccg ataggtcact cggggtacag gtgactcggg gacgggctgt gcccagatcg
      241 accaataggt cactcggggt acaggccggt tgggcgtcct tgctccggat tgctgggcct
      301 tgctcccgcc gcagatcgca actcccgatg tgcgcccttg cagcagcgca catcgggagc
      361 aagagcgcac atcgggagta aggatctgcg aggaggagcg gtcagccctc gggcagtgac
      421 ccgtgggccg gctccaggtc ggggtcgcgc aggggggtga gggtcgggcg cttggccgcg
      481 aggcggtctc ctgacgactc accacgaagc cggcgctgca cccacggggc ggcatgtgtc
      541 ctggcccact gggcgtgctc ccggaactcg tcgcgccggg cgcccctggt cgcgggcgcc
      601 agtggcgtcg cccactccgc cacgtcggtg ccgtgcccga gggccgtgag cgccgccagg
      661 gcaacccggc ggtggccctc gctgctgagg tggatgcggt cctcgcccca catccgccag
      721 tcacgcagcg ccgcgatgcc ccagaggttg agcacgtggc actcgtggcg ctgggcgatg
      781 gtgaagatgt tcgccgagtg cacggcgtgc cgcggtcgca gcgccttgaa cagcccggcg
      841 tcgcgggggt cggccggggt cgcgagcaac acgtcggcgc cggcccggcg aagccgcacc
      901 acggcatcct cgatgcgact ggccagcgag tcgaggtcga cgctcgggcg cagcaggtcg
      961 ttgccgccgc cgaccatcga gacgaggtcg ggggagagcg acagcgcact gtcgagctgc
     1021 gggccgacga cgtcgtcgag cttgcggccg cgcacggcga ggttcgcgta ggcgaagggc
     1081 acgtgggcct cctcggccac ggcgtcgagg tgcaccgcca ggcggtctgc ccacccgacg
     1141 taggcgccgg tcgtcacggg gtcggggtcg gacatcccct cggtgaacga gtcgccgatc
     1201 gcgacgtaac gggtccagac gggggtggtc acggaaggct ccagttcacg gggtcggcgc
     1261 cctgacgggc gagcaggtcg ttggtgcggc tgaaggggcg cgagccgaag aagccgttgc
     1321 gcgccgacag cgggctcggg tgcgcggact cgatgagggc aacgcccggc aggtgcggcg
     1381 ccaggccacg agcgtcgcgg ccccacagga tggcgacgag cgggccgccg cgctcgacca
     1441 gggccgagat cgcgcaggcg gtgacctctt cccagccgcg cccgcgatgg ctgcccggag
     1501 cacccggggc aaccgtgagg acgcggttga ggagcagcac cccctgctcg gcccacggcg
     1561 tgaggtcgcc ggtggctggt ctggtcaggc cgaggtcggc ctcgagctcg gtgaagatgt
     1621 ttgccaggct gcgtggcacc ggccgggtgt caggggcgac ggagaacgac agccccaccg
     1681 cgtggccggg tgtggggtag gggtcctggc cgacgacgag cacgcgcacg gcatccagcg
     1741 gttgctggaa ggctcgcagc acgttgggtc cgctcgggag gtaaccccgt ccgtcggcca
     1801 cctccgcccg caggaagtcg cccagcctcg cgacgaccgg ctcgacgggt gccagcgccc
     1861 gcgcccacga cgggtgcacg agatcacgca gggggcgcgc ggcatccgtc gaagtcatcg
     1921 aggccaccgt atctggctcg acctcgtgcg actcgggtgc ggtgctcggg agtttccggc
     1981 ctcgcggccg tcatcacgtc gcacgaggtc gggttcaggg cggtggggga ggccgggtgc
     2041 caggtggctg gagtgcaatg agtcggggaa gggggggcgt cagcggaaga tcgcggtcac
     2101 gtcgtcgagc cgtgcgggca ccaaccggaa ccaggcccac gtgccgcgct tctcgcgctc
     2161 gagcaggccg gcctcgacga gcaccttgag gtggtgcgac acggtgggct gggacagccc
     2221 gacgacgggg gtgaggtcgc agacgcaggc ctcgccgtcg gccgtggccc ggatgacgct
     2281 cagcagctgg agccgcacgg ggtcggcaac ggccttgagc agcgcggcgc tggtctcggc
     2341 ggcctcgcgg gtcaggccgc cgtcgacgcc gatgccgcag caggatgccg tggccgtggc
     2401 cgtggccgtg gccacggcgg aggtcgcggg ccgggtggca gttgtcgaca tgggggctcc
     2461 tcggtgcggc actacattga cgtgtgtcga tgtagtgtgg tccactgtga tcgcattgat
     2521 gatagtcgat atatgggagg ccattcgtga acacccctga cgccgtcgac ccggtcgctg
     2581 ctgcgggcgg cgcgagccgg gtccagctcg cgctcaacgt cagcgacctg gaggccagca
     2641 tcgccttcta ctccgccatg ttcggggtcg agccgcacaa gcgccgcccc ggctacgcca
     2701 acttcgcgat cgccgagccg ccgctgaagc tcgtgctcat cgagacgagc gaggccaccc
     2761 gcggcaccgg cacggctggc gccctgaacc acctcggcgt cgaggtgccc tcgaccgacg
     2821 acgtcaccgc cgcgcgcacc cggttcacgg atgccggcct ggccaccttc gacgagaacg
     2881 acaccacctg ctgctacgcc ctccaggaca aggtgtgggt ccacgacccg gccggggctc
     2941 cgtgggaggt ctacaccgtc aaggacgagg acccggtcga cgccaggccc gccacggcga
     3001 gcctggagat cctcggcggg ccgacggcag gtgcctgctg ctccccgagt gccgacgcca
     3061 cgccggtcga ttcggccccc gagaaggctc aggcctgctg ctcgtgacca ccgcatcccc
     3121 ggcgcgcgac acggcatccg ccggtcccga ccagccgaga cacctgtcca cccttgaccg
     3181 cttcctgccg gtgtggatcg gcctcgcgat gctcgcgggg ctcgtgctcg gccggctggt
     3241 gccggggctc ggggaggcgc tgtccgcggt cgaggtggag ggggtctcgc tccccatcgc
     3301 catcggcctg ctcgtcatga tgtacccggt gctggccaag gtgcgctacg accggctcga
     3361 cacggtgacc ggcgaccggc ggatgctcgg cgccagcctc gtgctcaact ggctggtcgg
     3421 cccggcgctg atgttcaccc tggcctggct cctgctgccc gacctgcccg agtaccgcac
     3481 cggcctgatc atcgtcgggc tggcgcgctg catcgccatg gtcatcatct ggaacgacct
     3541 cgcctgcggt gaccgggagg ccgcggccgt gctcgtcgcg ttcaactcgg tcttccaggt
     3601 gctgatgttc ggggtgctcg gctggttcta cctctcggtg ctgcccggct ggctcggcct
     3661 gccccaggcg gacctcgacg tctcgccgtg gcagatcgcc gggtcggtgc tcgtcttcct
     3721 cgggctgccg ctgctcgccg gctacctgtc gcgaacggtg ggtgagaagc gctgggggcg
     3781 aacggcatac gaagcgtcgt tcctgccgcg agtcggcccc tgggcgctct acgggctgct
     3841 gttcaccatc gtcatcctgt tcgcgctcca gggcgaccag atcacgacca gcccgctcga
     3901 cgtcgcgcgg atcgcgctgc cgctgctcgc gtacttcgcc ctcatgtggg gtggcggttt
     3961 cgccctcggg gcggcactcg gcatgacgta cgagcgcacc acgaccctgg ccttcacggc
     4021 ggccggcaac aacttcgagc tcgccatcgc cgtcgcgatc gccaccttcg gggtgacgtc
     4081 cgggcaggcc ctcgcggggg tcgtcgggcc cctgatcgag gtgccggtgc tcgtcggtct
     4141 cgtctacgtc tccctcgccc tgcgaaagag gttccgtcat gtctgacctg cccaccaccc
     4201 gcccgtcggt gctcttcgtg tgcatccaca acgccggccg gtcccagatg gcggccgcgt
     4261 gggtgcagca cctgtccggc ggcgccgtgg aggtgcgctc ggccggcagc gccccggccg
     4321 ggtcggtcaa cccggccgcc gtcgaggcga tgctcgagga gggcatcgac atgagcgcgg
     4381 aggtgccgaa gatcctgacc acggatgccg tgcgcgagtc cgatgtcgtc atcaccatgg
     4441 gctgtggtga cacctgtccg atcttcccgg gcaagcgcta cgaggactgg gccctcgagg
     4501 accccgcagg ccagggcgtg gatgccgtgc gtccgattcg cgacgagatc cgggtccgcg
     4561 tgctcggcct gctcgagagc ctgggtgtcc aacccgtcgc ctgaaccctg cgaggggttg
     4621 acagactgtc ccgatgcgcc cccgcttcca caccgtcctg ttcgacctcg acggcaccct
     4681 ggccgacacg attcccctca tcgtcgcctc gtaccagcac gccttccgca ccgtgctcgg
     4741 cgaggagatc gacgaggcca gggcccgcgc gtggatcggc cgcccgctgc tggccgcgct
     4801 gctcgaggag tcgcccgagc acggccacga cctcgaccag acgtaccgcg agtggaacct
     4861 cgccaacacc gcccgcctga tccggcgcta tgagggtgtc cccgagatgc tggaggccct
     4921 ggcctcagcc ggcgtgcgca tggcggtcgc gacctcgaag cggcgccaga ccgcacgcct
     4981 ggccctggag ggcgtcggta tcgaccacct catcgaggtc gcggccgcgc tggaggacac
     5041 cacccgccac aaacccgaac ccgacccgct gctgcacgcc gcggcatccc tgggcgccca
     5101 accctgcgac tgcgtctacg tgggtgacgc cacggtcgac gtcctcgctg cccgggccgc
     5161 cggcatggct gcgatcgcgg tgagctgggg tgcggccgac cgcgaggccc tcgcggccac
     5221 cgggcccgac gccctggtcg acaccgtcgc ggacctcacg gcgtacctgc tgggccacga
     5281 cgagggctag agtcgccgcc gtgcacggga agacctacca ggagaacaag cacgtcgagc
     5341 tggcggcact ggccgacgcg gtgatggcct acgccgagga gcgggtgcgc atggccccgc
     5401 cgctggaccg cccggtgacc gaggccgaac tgcgctcgcg ggtcggggag acgatcaccc
     5461 ccggcggact cggcggcgtc gaggccatgc gtctgttcgc gcaggacctc gcccccacct
     5521 gcctgtcgac cgaccacccg cgctacctgt ccttcatccc gtgcgcgccg agtgacgccg
     5581 ccgtgatgtt cgacctcgtc gtcggggcgt cctcgatcta cgccggctcc tggctcgagg
     5641 gctcgggtgc cgtgtacgcc gagaaccagg cgctgcgctg gatcgccgac ctcgtcggga
     5701 tgccgtcggg cgcaggcggg gtgttcaccc ccggcgggac gatcggcaac ctctcggcgc
     5761 tggtgactgc acggcacacc gcgcgcgcct ccgcgaagcc gggcgaccgg ccgtaccggg
     5821 tggcagccac ggccggggcg cactcgtcga tcgcgtcggc ctgcgacgtc atggacgcgc
     5881 agttcgtcgg cgtccccgcc gacgacgcgt ggcgactgac cggcgagaac ctgcgggcgg
     5941 tgctcgagga gaacggcccg gagacgttct tcgccgtcgt cgcgacgtgc ggcacgacga
     6001 acttcggtgt catcgacgac ctggcgagcg tggccgaggt ctgccgcgag tacggcatct
     6061 ggttccacgt cgacggtgcc tacggcgggg ccggcctggc cgcgccctcg gtgcggggcc
     6121 tgtacgccgg gatcgagcac tgcgactcct tcatcgtcga cccgcacaag tggctcttcg
     6181 cgccgttcga ctgctgcgcc ctgctgtacc gggagccgtc gctggcgcgg gcggcccaca
     6241 cccagaaggc gggctacctc gacgtcctca ccgacgcccc cgactggaac cccacggact
     6301 actcggtggg actgacccgg cgggcccgtg ggctgccgtt ctggttctcg ctcgtcgtca
     6361 acggcagcga cgcctacgag gccgccatcg agcacaccct ggacgtcgcg cggttcgccg
     6421 aggcggaggt gctgcgccgc cccgacctcg agctggtgcg cgagcgggac ctgtcggtcg
     6481 tcgtcttccg acggctgggc tgggagcccg cggactacgc cgcgtggtcg gaccggatgc
     6541 tggccgagga gttcgccttc gtcgtgccga cgagccacga cggcgagacg ctggcccgct
     6601 tcgccatcgt caacccggag acgagcgagg acgacatcac cgccatcctc gacaccatgg
     6661 actgacccct cctcgggcgt ccgctgagcg gcagcctggc ggggtccgtg gtgctcgtgt
     6721 gacgggtgcg gcgggggtgt cgtgaccggt tcgtggccgg tggcccgacg cgcggaacgg
     6781 gagcccgggc gaatgacagg cgtgtgattt cccggctaca gtgggcgctc acgcagggtc
     6841 agcaacgaag ggtcgaggaa cacacgtgag cgccgcctcc gaacagctca gcaccgccgc
     6901 gcccgcggcc gggctcaccg agcgcgaccg cgagatcatc gccttcgaac ggcagtggtg
     6961 gaagtacgcg ggctcgaagg agaccgccat caaggagctg ttcgacatga gctccacccg
     7021 gtactaccag gtgctcaacg ccctcatcga ccaccccggc gccctcgagc acgacccgat
     7081 gctcatcaag cggcttcgcc gcctgcgcgc cagccgccag cgggcgcgct ctgcccgccg
     7141 cctcggcatg gagccctgag ccccggcccc aagaaccagc gacgcccgtc cgtgagggaa
     7201 gccggacggg cgttgctgta cctgcggtga cctgttggtc ggtcggggga cgnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggtccgg gaagtccacc ggccacccct
     7381 tggcggccaa ccggtcgcgc acgcgccgca ggtcgtccgg cgtggcctcg gagtgggtga
     7441 ggtcggcgat ggccgccgcg atgtcgtcag ggctggccgg ggcgatggac gcccggcgca
     7501 gggccttggc gaccttcttg acctcgccgc gcgtcaggcg tcgctcgagc agggcgaaca
     7561 gggggacgta gtcgtgctcg gggaccccgg tggggtagcc ctcgcgcagc cacgcgacga
     7621 tgcggcccag gtaggagccg ggctcggtgt cgtcctcgtc gtcgaggtcg tcaccgggcg
     7681 ggttgccgag cggccacccg cccaggacca gtcgggcggc aaccctgcgc aggtcactct
     7741 gcggcagtgg agccttcggg gcgctcccgg atgctgcgtg gtcggcgctc agcgctccgc
     7801 cggcgaccag cgccagcgcg accgacagcg tgcgggtgga tccgatgcgc tgacgcagca
     7861 gcgcgaggag gggcgcgaca tcctcttccg ggatgccgtc ggggtaggcc tcggcgaggt
     7921 acctgagggc ttggctgttc gtgtcgacca tggtgtgggt gggattccct ctcgttactt
     7981 gtcgacgatc gtcggcagga tgccgtcgcc gctgaagctc accgtcttgc cgaagccgga
     8041 ggcggcgatc atcgtgatgc cgagagcgac ggacgcgacg atcacggcca ggagcacgcc
     8101 tgcgacggcc cggccgagca cggtggggtg gaaggtggtg tggccgctgg cgtcctcggt
     8161 ctccgtgctc ccggtgacga gagcgcgcat cgcgagcgca tagaccacgg ggaggccggc
     8221 gccgaagatc aggccggcga agaggacact ggccgcgccc tggagtgcaa gttcgatgct
     8281 gcgcattgct gggtcctttc ggtcagtggc tcttggtgcg gtgggtcgtg gtgccggccg
     8341 ggtcctcggg cgtctcgggt gcgggcggga acttgtcgag gacgtcgtcg gcggtgtccc
     8401 actcgtcgtt gacgttgtga gcgccgacgt tgtcctgctt ggaccgccag aacatgtacg
     8461 ccgaggcgag gccgagcacg atgaacacga tgaccgcgcc gagcgctcca ccgatgctgt
     8521 tcccgagcca ccacgtgagg gcgccgacca gggccgcgag ggggaaggtc gtcgcccacg
     8581 cggtgaccat gcgcccggcg gtgttccagc gcacgttggt gccccgcccg agtccgccgc
     8641 cgaggacgga tccggtggcg acgtgggtgg tggagagggc gaagccgagg tggctcgcag
     8701 cgagcagggt cgctgcggtg gcgccgtcgc tggccatgcc ctggcgaggg gtgatgtcga
     8761 ccagaccctt gccgagggtg cggatgatgc gccagccgcc caggtaggta ccggcggcga
     8821 tcgacagggc agcactgacc ttgacccacc aggggaccga ggtggtgtcg gtccagtggc
     8881 cgctcgcgag cagggccagg gtcatgaccc ccatcgtctt ctgcgcgtcg ttggtgccgt
     8941 gggcgaggga gaccagcgag gcgctgccga tctggccgta gcggaaggtg ccctcaccga
     9001 gcttgtggac gatcgagttg gtgatgcggt ggacgagcca gcacccgatg aaggcgacga
     9061 tcagggcgat caccggggac aggaagctcg gcagcaggac cttgccgacg acgccgtcga
     9121 gcttggtgcc gtcaccggtc cacttcacac cggacagccc gtatgccgcc gcggcggcac
     9181 ccgtgagccc accgagcagg gcgtgcgacg aactcgacgg caaccccagc agccaggtga
     9241 acaggttcca gatgatccca ccgacgagtc cgcacaacac cacgagcagc agcccctggc
     9301 cgccgccgga gaggaactcc tccttcggtg cgccggactt gtcctggatg ttgatgacgg
     9361 cgttggacac cgtcagtgcc acctcgacgg agaggaaggc gccgacgagg ttgaggatgg
     9421 ccgccagggc caccgcagcc ttcggcttca gggctcccgt ggagatggag gcggccatgg
     9481 cattggccgt gtcatgaaag ccgttcgtga agtcgaaggc cagtgctgtc acgatgacga
     9541 gcacgagagt gagggtcgca gcgtccacga tccttcagtc tggtcccgaa accgtgagtt
     9601 cgccaagcag aggggtgttg ttcatctctc gttcatcatc gctcgcggca cctgccggtc
     9661 cacgcgatga cacgatgcgc cgatgagtga cctggactgg agcacggtcg acgggctcgc
     9721 cgccatccgt gagcacctcg ctgcccggat cgaggggtgg cgccagccgg tggcgtatgc
     9781 cgtcgctctc agtcccgcgt cgtcctcgcc ggagtgggac ttcgcccacg tcaacacccc
     9841 cgacgggcgc cacgggctgc ccgcggtggt gctcgcgacg atcctcggcc acgacggctc
     9901 gaccgcctcg ctcccggtgt cggcgcacca gctggccgcg gcggtcgagt cactggcccc
     9961 tgcgcaggcc tgcacggcgg tggaccaccc caatctcgcg gcctggcgtg cggccctggc
    10021 cgacctcgag ggcaatccgg cccgggaggc ggtggccgtc ttcgtggccg acctccacga
    10081 cccggtgacc tccgaggccg acgggtcgat gcgtgcgacg ttcgaaggcc acgcccccgc
    10141 gctctgaacg ccgacgcggc aggggacgaa acggcatcct cggtcgttgg gactcccggc
    10201 cggatgcgtg cggggctccc gcgcgtgatc gacgggcccg tggacgtcgg aggtagcccg
    10261 tgctcgacac gctcaccgcg ctcgtcgagg ctggtgcggc cgcgacgtgg gtgctgcccg
    10321 tggtgctgct cgtcgccgtg ttcgacgccc tggtcccgcc ggtgccgagt gaggggatcg
    10381 tcgtcgccct ggccgctgtc gccgtcgccg gtgacgggcc gaacctgatg gccctcgcgg
    10441 tggccgccgg cgtcggggcc ttcctcggtg actccctgac ctacgtcgtc ggtcggcggt
    10501 tcggtccgcg acgactgcac cgcatcaccc ggcctgccgc gcggcgggcc ctggagcggg
    10561 cctcggacac gctcgaccgg cgcggtgccg tcgtcatcct cacggcccgc tacgtgccgc
    10621 tcggccgggt cgccgtgaac ctcaccgcgg gggcgaccgg tttcccaccc cgccggttct
    10681 tcgggctggc ggcggtggcc gcgacgacgt gggccgcctg gtcggtcggg gtgggggcgc
    10741 tcgccggcca ctggctcgag ggcaacccgc tgctcggctc ggtgttgggc gtggtggcgg
    10801 ccctgctcct cggcctcgtg atcgaccggg tcgcccggcg cgcgagcggg tggggccgga
    10861 tgccgtccgc ggtcgtcgcg cgcacctgac ctccccgtcg cccggcaggg ccggtaccgg
    10921 tcaccgcccg ctcgggggcg gcccggcgtt tgcactctca tggtgagagt gccaataatg
    10981 gcgttagcac tctcaccatg agagtgacaa tcctgaccgg tccggtgagg gtggggtgcc
    11041 ccggcgcgtg agctgacggc atccgactcg tccgtcgcgg gcgccgcccg gtctccacct
    11101 tcgattcgcg tggaggaacc acccgaatgg ccaagatcat cgcttttgat gaagaggcgc
    11161 gccgcggtct cgagcggggc atgaacgtgc tcgccgacgc cgtcagggtc accctggggc
    11221 ccaagggccg caacgtcgtg ctcgagaaga agtggggcgc ccccacgatc accaacgacg
    11281 gcgtctcgat cgccaaggag atcgagctcg acgacccgta cgagaagatc ggcgccgagc
    11341 tcgtcaagga ggtcgccaag aagaccgacg acgtcgccgg tgacggcacg acgacggcga
    11401 ccgtcctcgc ccaggcgctc gtccgcgagg gcctgcgcaa cgtcgccgcc ggggccaacc
    11461 cgatggcgct caagcgcggc atcgagaagg ccgtcgcggc cgtctccgac gagctgctcg
    11521 ccgccgccaa ggacgtcgag accaaggagc agattgccgc gaccgcgtcg atcagcgccg
    11581 ctgaccccct gatcggcgag atgatcgccg aggccatgga caaggtcggc aaggaaggcg
    11641 tcatcaccgt cgaggagagc aacaccttcg gcctcgagct cgagctcacc gagggcatgc
    11701 gcttcgacaa gggctacatc agccactact tcgtcaccga caccgagcgc atggaggccg
    11761 tgctcgagga cccctacgtc ctcatcgcga actccaagat cgggtcgatc aaggacctgc
    11821 tcccgctcct ggagaaggtc atgcagtcgg gcaagccgct gctcatcatc tccgaggacg
    11881 tcgagggcga ggccctgtcc acgctcgtcg tgaacaagat ccgtggcacc ttcaagtcgg
    11941 tcgccgtcaa ggcccccggc ttcggtgacc gccgcaaggc catgctcggc gacatcgcca
    12001 tcctcaccgg tggccaggtc atctccgagg aggtcggcct caagctcgac accgccgagc
    12061 tcgacctgct cggtcgcgcc cgcaaggtcg tcgtcaccaa ggacgagacg accatcgtcg
    12121 agggtgccgg tgacgaggag cagatcgccg gtcgggtcaa ccagatccgt gccgagatcg
    12181 agaagtcgga ctccgactac gaccgcgaga agctccagga gcgcctcgcc aagctcgccg
    12241 gcggcgtcgc cgtcatcaag gcgggcgcgg ccaccgaggt cgagctcaag gagcgcaagc
    12301 accgcatcga ggatgccgtg cgcaacgcca aggccgccgt cgaggagggc atcgtcgccg
    12361 gtggtggcgt cgccctcatc caggccggca aggccgcctt cgccaagctc tcgctcgagg
    12421 gtgaggaggc gaccggtgcg aacatcgtgc gcgtcgccat cgaggccccg ctcaagcaga
    12481 tcgcgatcaa cgccggtctc gagggtggag tcgtcgcgga gaaggtcagc aacctcgact
    12541 ccggttgggg cctgaacgcc gcgaccggcg agtacgtcga cctcatcgcc gcgggcatca
    12601 tcgacccggc caaggtcacg cgctcggccc tgatgaacgc tgcgtcgatc gccgcgctgt
    12661 tcctcaccac cgaggccgtc atcgccgaca agccggagaa gaacccgccg gccatgcccg
    12721 gcggcgacga catgggcggc atgggtttct gacccgagcc acccaggtct caccgcacga
    12781 gggcggtccc cacaccgggg gccgccctcg tggcatgtcc gtcaggtcat gcgcaggccg
    12841 cggccctggt cgaccggggt ccgggcccgc agcagcaacc gcaccgcagt gcccagccac
    12901 agggcggcca gggccaccgc gaccaccagg ctcgaggtcg tcggcagctc gtcacggccg
    12961 agcagcagca ggggcacgcc cagcacaccg aaggcgacca cggccagcgc cctgtcggcg
    13021 gcgccccagc gccgcgagag cagcacgagc acacagccga tcagccacgg cagcggtgcg
    13081 aacgccgagg tcgccggcac gagcgccagg acgaccgatg ccgcgagcag cgtgatcgtc
    13141 acgacctcca gccacggggg cccggtcacc ggggcgccga acgggtcggg cgcgaggtac
    13201 tcgatctcgg gagccaccgc ctggccgctg gcctcggcga cgaggtcggc aggatccccg
    13261 aggtcggcga gcacctggcg aaccgcggcc tccgagcggg gggtggtgcc gacggcatcc
    13321 gcgaggtact cgtcgatgct cgtgcgcagc tcggcccgcc gctccgccgg cagcgccgac
    13381 gcctcggtgt cgagtcgggc gagccactcg tcgaccgccg ggtggttcgg ggtcgtcatg
    13441 tccgcgcgcc tgccatggcg gacacggtag ccgccacgta gacgccgtgc gctcagcaac
    13501 cattcagggg gtgcccagca tctggtggga aaggcgaggc aggatcgacc catgaccacg
    13561 gccaccgcca ctgacccgct cgtcctcgga cccctgctgc gctacgtcga cgagtcgtcg
    13621 gcgacgatct gggttcgcac ggcggatccc gcgctggtca ccgtcgagcg ggccgggcac
    13681 gtctggagtg ccccgacctt cgccgtgcac gggtcgcact tcgcgctcgt cgtcctcgac
    13741 gggctcgagc ccggcagcga cgacgcgtac gaggtccgcc tggacggcac ccccgtctgg
    13801 cctcgtgagg ggatgccgcc gagccgggtt cgcacgctgg acccgacccg cgaaccgcga
    13861 ctcgccttcg gcacctgccg cacgaccggg agccacgacg aggagggcaa ccgggcccac
    13921 ggggtcgatg cgctgcgctc cttcgcgatt gccctgaggg acgacccggc gacggactgg
    13981 cccgacctgc tcctcctcct cggcgaccag gtctacgcgg acaccacgcc gcaccccgag
    14041 ctcgaggagt tcatggcggc gcggcgggac ctgtcgctgc ctcccggtga cgagatcaag
    14101 gacttcgtcg agtacgccga gctgtaccgg ctcgcgtgga gcgacgacgt cgtgcgctgg
    14161 gtgctgtcga cggtgccctc ggcgatgatg ttcgacgacc acgacatccg cgacgactgg
    14221 aacacgtcgt ggtcgtggcg gcgcgagatc cgggccacga cgtggtggca ggagcgcatc
    14281 gtctccgggc tcggggccta ctgggtgcac cagcacatcg gcaacctctc gccggccgag
    14341 ctcgccgacg aggaggtgtg gcgccacgtg agcgcgcacg cggcatccgg ggagcctggt
    14401 gagctcgacc tctcggacgt cctggatgcc gtggccgccc gagcggacga ggaaccgtcg
    14461 acgtaccgct ggagctacgc gcgcgccctc ggggactgcg ccctggtcgt cgtcgactcg
    14521 cgcgcggccc gtgaactgcg ccccgacggc cgctcgatgc tcgacgccga cgagatgcgc
    14581 tggctcgacg cggtgatgcg cggaggggtg cgccacctgt tcatcggcac ctcgctgccg
    14641 ttcctcctcc cgcccggtct gcacgacttc gaggcgatga acgaggccat ggcgcagggg
    14701 tcgcacgggc ggcgggtggc gaggggcgcc gagcgggttc gccgccgcat cgacctcgag
    14761 cactgggcgg cgttcaacga gggcttcgac gaggtgttcg agatggtcat ggcggtcgcc
    14821 cgtggtgagc ggggcgcggc gccacagacc gtgacgttcc tgtcggggga cgtgcacaac
    14881 tcctacctgg cgcaggtgag cgaccccgaa cgcctcggtg cctcgtcgcg catcgtgcag
    14941 gcggtgtgct ccccgatccg caaccccatg ccgcgcgccg tgcgggtcgt catgtcgctc
    15001 ttcgcccgct cgctcgtgcg cccgatgagg ttcatcgccg ccaggtcacg gcgcgtgccg
    15061 gacccggcgt acccgtggac ggtgaccgac gggccgtggt tcgacaacaa cctcgccctc
    15121 gtcacggtcg gtgagcgttc cctcgacctg acgtgggtca cgggggtcgt cgtcgatgac
    15181 gtcgaccgcc cggccttgca gaccgtgtat gccgcacaca tcacgccgtt ggcgtcgtca
    15241 ccacggttgg tcgagggtct gtctaaggtt tgacagcctg cgcggaacac ggcggggctc
    15301 gcctaccgtt gggcgggcag tgggtgcgcg tgtgtgccct cctgaccccc tgccgggttt
    15361 catgccggcc acgctgacaa ggattcccat gaccctcgcc gaaaccaccg cccgcgagac
    15421 gacgccgcgc ctggccgccg cccgggtcga ggggctgagc aaggtgtacg gctcggggga
    15481 gacccgggtc gtcgccctgg acgacgtgac cctcgacctg tacgcggggg agttcaccgc
    15541 gatcatgggc aagtccgggt cggggaagtc cacgctgatg cactgtgcgg ctgggttgga
    15601 cgaggccagc gctggccacg tcttcgtcgg ggacgtcgac ctcaccacgc tcaaggagcg
    15661 ggcgctcacc gaactgcggc gcgaccgcat cgggttcgtc ttccaggcct tcaacctcgt
    15721 gcccacgctc accgcccgcg agaacatcgt gctcccggcg gccatcgccg gacgcgagcc
    15781 ggatgccgcg tggttcgacg aggtcgtcga caccgtcggg ttgcgcgacc gactcgggca
    15841 ccgcccgagc gagatgtccg gggggcagca gcagcgcgtc gcctgcgccc gcgcgctcgt
    15901 cgggaggccc gacatcgtct tcgccgacga gccgaccggc aacctcgact ccacgtcggg
    15961 cgccgaggtg ctgggcttcc tgcggcgggt cgtcgacacc cacggccaga ccgtggtcat
    16021 ggtgacgcac gaccccggtg cggccagctg gaccgaccgg gtcgtcttcc tcgccgacgg
    16081 caaggtggtc gacgagctgc gtgagccgac ccccgagctg gtcctggaga agatggccgc
    16141 cctcggtcgg ccggtcgggg cctgacccgt gctcaaggcc acctggcgca atgtcttcgc
    16201 gcacaagctg cggctgttcc tcagcgcctt cgccgtcatc ctcggtgtcg cgttcgtcgc
    16261 cggctcctac atcttcaccg acaccctcga ccgagccttc accggtctga cgagcggggc
    16321 cgtcggggac gtcatcgtgc agcccgggtc gagcgatgac gaggaggcgt ccggtccggg
    16381 cgccctcgga acacgcaccg ttccggcctc cctcgtcgat gagctgggag cagtgcccgg
    16441 ggcggcccgc gcggacgggc gcgtgtcgaa cttcggcacg ttcgtcgtcg gcaaggacgg
    16501 caagctcatc gggggccagg gcccgcccgg gatcgccgtc aaccgcaccg aggggccggc
    16561 cgcgaacggc atcccctcag ctacgctgga gagcggacgc tggcccgagg cacccgacga
    16621 ggtcgtgctc gacccgtcca cggcacgcaa ggccggctac ctgctggggg agcggatccc
    16681 gctcgtcacc acgagcgagg tgccccgtat cgaggtgacc tatgtgggga ccgcgagctt
    16741 cggcgcctcg gctctggtcg gtgccagcgt cgtcatgctc gagacctcga aggctcagga
    16801 ggtctacctc ggcggtgagg acgccttctc cgccgtgtgg gtgaccgcgg cgccgggcac
    16861 gagccaggag cagttgctcg cctcggtcaa ggaggtcctg cccgaggggt tccgggccgc
    16921 gaccggcgac gcgacctccg agcgcgcctc gacccgaatc gacgaggccc tgtccttcgt
    16981 cacgacgttc ctcctcgtct tcgccggggt cgccctgacc gtcggcgcct tcctcatcgt
    17041 caacaccttc tcgatcctcg tcgcccagcg cagccgtgag ctggcgctgc tgcgggccat
    17101 cggtgccagc cgacgccagg tggcgcggtc ggtgcttgcg gaggccggcg tcgtgggcct
    17161 cgtcggatcc gccgtgggta tcgcgatggg cttcgtgctc gccgtcggga tcaagttgct
    17221 cttcagtcgg atcgggctcg acctcagcgc caacgagctc gtgctgcggc cgcgcacggt
    17281 cgtcgtggca ctcgtggtcg gcatcctcgt caccctggcc gccgcgtacc tgccggctcg
    17341 tcgggctggc cgggttcccc cggtcgcggc gatgcgggac gacgtcgccc tcgccgagag
    17401 cgggctgcgc tggcgcctcg tcgtgggtgc ggcgctcctc gccgccggga tcgcggccat
    17461 ggcggccggg ctcgccggcc tcgggtcgca gcccacgtac gtcctcggtg cgggcgcgtt
    17521 cggtgtcctc gtcggcacgg cgctgctgag ccccgtgctc ggccggccgg tgctggccgc
    17581 cctggggtgg ttctaccgcc gctccttcgg agccgtgggg ctgatggccg agcagaacgc
    17641 ccgccgcaac ccgcgacgca cggcggccac ggcatccgcc ctgatgatcg gtgtcgccct
    17701 ggtgacgatg atgtccgtgc tcggtgcctc ggccaaggcc agcctggacc agtcgctctc
    17761 cgaggacatc gtggccgact tcgtcgtgtc caacgccgtg gggcaggcct tctcctcgac
    17821 ggtcgctgac gagatcgagc aggtcgacgg ggttgccgcg gtcgcccggg tgcgcagtgc
    17881 ggtgctccag gtcgacggcg accgcgactt cgcgtcggcg gtcgacccgg cggccgtgga
    17941 tgccgtggca cggccggaga tcgtcaccgg caccctggct gatctcgacg cgacctccgt
    18001 ggcgatctcg agtgagttgg ctgcggatcg gggctgggcg gtcgggtcga ccgtcaagat
    18061 cgggtacgca ggtgcgacga cggacgccac ggtcgtcgcg acctatgtcg agggtgccgt
    18121 gctgcagtcg gacgtcacga tgtcgctgga gggcttcgac gccatcgggg tgccgccgac
    18181 cgaccgcacc gtctacgtcg tggcagcgcc cggggtcgac cgggctgtgc tgggagctgc
    18241 gctgaaggac gtcgtggtcg acctgccgac cgtcacggtc aacgaccagg agaccttcgc
    18301 cgaggagcag cgcgcgccga tcgaccagct gctgttcatc gtctacgccc tgctcggcct
    18361 ggccgtggtc atcgccgtgc tcggcatcgt caacacgctg gccctgtcgg tcatcgagcg
    18421 ggtgcgtgag atcgggctgc tgcgggcggt gggcctcagc cgcacccagt tgcgcacgat
    18481 gctgcggctg gagtcggtgg cgatcgcgct gctcggggcg gtgctgggca tcgtgctcgg
    18541 actcgcgttc gccctggcgc tccagcgctc gctcatcgac gacggcatcg acgtgctcgc
    18601 catccccgtc gggcagttgg cgctgttcgt cgcggtggct gccgtggtcg gggtgctcgc
    18661 cgccctgtgg ccgggccgac gcgcggccaa gctcgacatc ctgcgcgcca tcgccagcga
    18721 ctgaccggct cagccgagct cggcgggggg ccgagctcgg cggggggccg agctcggctg
    18781 atgagcgcgg tcagggagtg aacatccgcg tggctgcggc ctcggcgtcg gcgtactggg
    18841 tgcccgcggc gccgagcacc tggccgatgg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnggcctcgg cgtcggcgta ctgggtgccc gcggcgccga gcacctggcc
    19021 gatggagtcc agcgaggtgc gcacctgctg ctgcgtgccc cgccactgcg agacgacacc
    19081 ctggaactgc gccgacgccg tgccggtcca cgcgtcctga aggccgttga gccgggccat
    19141 catcgtggcg acctggccct cgatctcggc ggagatgcgc gcgatgtcgc cggctgcacc
    19201 cgcgatgcgc tcggtgtcga cggtgaactg tgctgccatg ggaatccccc tcgttcaggg
    19261 ctgcacctga gtggcgcacc cgctgctgac gacactagga cgatcgccga caccgccccg
    19321 aaagttatcc acagggctgg tgcgaggggc aggcgaggtg gggggacggc gcggtgtcgt
    19381 cgcaggtcct tgttccggtc gcagatcctt gctccgccgg tgagcccttg cagcagcgca
    19441 catcgggagc agggactcac atcgggagca aggatctgcc acgaggtccg cgcggcaccc
    19501 caaccagggc ccaggtgccg cctcggccag cgtgaacggg tcggccggcg aacgatcggc
    19561 accaggtccg ggcacccgcc tacgatcggg gcgtggccat cctcatcgac ccgcctcgcc
    19621 ccgaggccca cggccgccgg tggtcgcacc tgatcagcga cacgagcacc gaggaactgc
    19681 acgccttcgc ccgcgcggcc ggcatccccc gcagggcgtt cgagggcgat cactacgaca
    19741 tccccgagga gcgctacgcg gccgtcgtcg ccgctggcgc ggagcccacg ccggcacgag
    19801 acctgctccg agccctgcag cgaagcggcc tgcgcctgca gaagcgccgc ggcgacaccg
    19861 gggtcgccaa gatcaccggc atccgcttcg tcgacggcac gagtgccgac gtcgacctca
    19921 tcgcctcgcc ccgcgaggcc gacgtggggc gggtgttcgc ggcgatggcc ttcgtccgcg
    19981 acgacaccgg cgacttcgct ctcgtgcact ccgtgcgccg cagcgagtgg ggtgcaccgg
    20041 gtggctggcg cgagccgggg gagtcggtgc gagagaacgc agttcgtgag gtccgtgagg
    20101 agaccggcct gcacgtcccc ccgcgcgacc tcaccccgat cggctacgag cggttccacc
    20161 accgatcctc ggggggcctg tggcgcgagg ggcaggacct gctccaggtc tacgaggtga
    20221 gcgtccccgg catccgaccc gccctcaccc ctgaactcga cgacacgtca caccatcagt
    20281 gggtcacctg ggacgagctc gaggccctgt gctcccgcca gttctggtgg gcgctagccg
    20341 aggcgaccct cagcccgcca gccgggtgag ttcgcgggcg aggttctcac gcgccgccga
    20401 cccccacgtc gaccgcgcgt actcggtgcg gtacacgttg tcccgcgcga ggaacggtcg
    20461 cagcacggca gagcgctcac gggcgtagac cgccgcgggt acgtgcgcgt actcggcgcg
    20521 cacctgggcg cagtactcgt cgaaccgaac caccggagcc gcgaggatcc acaggtccgc
    20581 gtcatgcagc agcacccggg cagggtcctc accggcggtg ctgcccagat cgtggtgctc
    20641 ggtgtcgagc acggatgccg tgacgcgctg tcgcgtcgcc gccgccaccc ccagcgcgct
    20701 caggtggtgc tcggccaggt ctgcgctcgc cgcctcgttg ctcacggcgg ctgtccttgc
    20761 ggccgctgct gcccccgacg tgccgggacg gtctggcgtc gcaggggtga ccgagtagac
    20821 ggcgtcgtgg aaccacgcgg ccaaagcggc gaccgttgcg tcctcggcgg acacggcacg
    20881 ggtggcgcac agccggtcga gcgcggcgag cacctcgctg aggtgggtga ggccgtggta
    20941 gcgccggtgc tcctcgctcc agcgagtgag caggtcgtgc cccacgtcgt cgacgacctc
    21001 ccgcgcagcc aggggagcgg cgacggcgca gtcggccacc caccggcgtt ggagcgcgcg
    21061 catcgggcca ctgtgccacc caccacgggc tcgcgtcacg agggtggccg cgaccgctcg
    21121 tacgccttgg cgtagcggct gcccatcgcc agcagcagca gacccacggc gatgaaggcg
    21181 accgagcgaa cgatgccgct cagcgacgac aggtcgtaga ggaacagctt cgccacgctg
    21241 acggcggcga gcaggaggcc cgtgcgtagc gtgaggtcgg cgtcaggcga acgctcgagg
    21301 ccgtgcagca gcaaccatgc ggctcccagc atccaggtga tcgtggcgac ggcctgcccg
    21361 atggtgaacc ccagggtcgg gtcgcccagt cgcgcgccga cgagcgtgcc agccgcggcc
    21421 agggccgtcg cggaggcggc cagccccagc acccagacgg cgatccgcac catcacgcgc
    21481 agctcacgcc ccagaccgcg ttgctggccc atcgcccaga cgaccatggc gagcaccccg
    21541 gcgagcacga tgctgtccag cagcgccgcc gccatgtcgc cgcgcagggt gcgcgcagac
    21601 gccgcgacgg tcagcgggta ctgcagccac ccgacgacgc ccaggagggt cacgcctgcc
    21661 gccagcacga ggttcacccg cgaacgcgtg attcctgcga cggccagacc cacgatcgcg
    21721 agcagcagca acccggtcgt gatgaaccgg gtcggtgccc ccgcgacgac cgccagcacg
    21781 gcgaacgcgg tgcccgccgc gcccgtggtg acgaccaggt ggccggccac cgggccgacc
    21841 gggccgcgac ccaacgtggt tgccgtgagg aggagcacgg cggatgccgt ggcgagggaa
    21901 agggtgcgcg ccgggttgtc gaaggtcgtc gtcgcggcga acagcccgat cgccagcagg
    21961 gcgaccgcga tggaggcggc caggtcgccg ggatcgcggc gcaccgacag ggtcgaggtg
    22021 aacagggccg cggcgagggc gatgccggcg atcgcgaggt tgccgacgtc gtccaccgtg
    22081 ccacctccgg ccaccgagct cacgagcagc gccaacgtca gcggaagcac ccgggccagc
    22141 gtgagcaccg gggtggtccg gtcgcccgac gcccaccagc cggcgaggca gaggatggcg
    22201 aggtagccgg tgaccaccca gccaccgccc tgggcgacca cgggagccag cacggccgct
    22261 ccgaggacga cgagcaccgc gagcagctcg ctgcgccagc tgcgggcgag gtgcaacccg
    22321 ccgagtgcga cggcaccggc gagggccaga cccggtgcgg tgggcaccca gccgtagccc
    22381 gtggtcacgg cgacgacgac aaggtaggcg gtggcgacac cggtggccac cagggcgacg
    22441 ggcgctgctc cgacccctga gggtgcgccc acggccgctg cgggtgcgcc ctcgacggtg
    22501 tcgcgcccac gaagatcggc gcgaccactg cgcacgccca gcccgatgag gatcacggcc
    22561 aggacggctc cggcggcgac tcgcgcctcc gggccgaacc agccgcgttg ggcggccagg
    22621 acgaggaaca tcgcgacccc ggccatggtg atgacggcac ccgcgatggc cagcacccgc
    22681 gtcaccgcac cctcgcggcg gtaccacggc accgacgggg tgggggtctg ccacggaccc
    22741 agcgttgccg gcggcgccgg tacctgcgcg gtgatgggcg gcgcggcggg taccgccggg
    22801 ggacctgcgg ggcgggcgac gggaggggga acgggagcag ggggggcggc atccgggatg
    22861 ccgtacccct cctcgcgctc gagctggctg cgcaggcgcg ccaggccgtt gccgacggtg
    22921 tacatgcggg tcatcgcgtc ggcgaactct gcttcgagct gctggacctg ggcgcgggtg
    22981 ctcatgggca cagtctcgtg cccgggcggc atgctcacct gagcactccc actcattccg
    23041 cgacgtcagg tgcaccccgc gcgctcaggg ccgtgatcgg ccctgacccg gcggggtgca
    23101 cctgacgtcg ggtcagggag aggtggtggt gggcttggcg gcgagggtgg tcttgacgtc
    23161 gatcgtcttg ccgtcgcgca ggacggtgag ggtgacctcg tcgccagcgc tgcgctcgcg
    23221 gatgtgggcg acgagggaga gggccgactc gacccgctcg ccacccacgg ccgtcacgac
    23281 gtcgccgacc tggagcccgg ccttggaggc gggggtgtcg gcaccgaccg aggtgatctc
    23341 ggcgccggtg cgcgtggccg agccgtcgga ggccgagccg tcctgcgggg tcaccccgag
    23401 gtaggcgtgc acggccgtgc ccttggcgat gagctgctcg gcgatcgagg tggcctcgtt
    23461 gaccgggatc gcgaagccga tgccgatgtt gcccgactga cccgaggacg acccgagcga
    23521 ggcgatggag gagttgatgc cgatgagctg tccgccggcg ttgacgagcg ccccaccact
    23581 gttgccgggg ttgatcgcag cagaggtctg gatggcgttg gtgacgacgg gctcggcctg
    23641 cgactgggcc tggccgaact ggtcctggcc gctgctctca ccctgggtgg tcacgggccg
    23701 gttcaaggcg ctcacgatgc cggtggtgac ggtgccggcg agaccgagcg ggttgccgac
    23761 ggccatgacc gggtcgccga cagcgatggc gtcggagtca cccacggcga tgggcgtgag
    23821 gttgctgggg ttgcccgtga tggtgatgac ggccaggtcg gtcgaggggt ccgtgccgcg
    23881 gatctcggcc gcgaaggtgc gaccgtccga ggtcgtcacg gtcagctcct gggcaccggc
    23941 caccacatgg ttgttggtca gcacgtggcc ggaggagtcg atgatgaccc cggagccctg
    24001 gccgccgccc tgcgcggagg tcgcggtgat ggcgacgacg ctcggggaga ccgcgctggc
    24061 ggtcgcggtc cagttcggcg ccgaaccgtc ggcctggacg accggcgcgg catcggcgtc
    24121 gcggccgagg gactgcgacg tccccgtggt ggtcgcggcg tcggtgccgt cgtaggcgcc
    24181 ggtgatggcg agcgtgctgc ccgaggcgat gaggcccgtg atgaccgcga cggcggtcag
    24241 ctcagccagg cggcggccgc gcctgggtgc cggaggagtg gtcatcgccg gcatcatgga
    24301 gttacccgga ggggctggcg gcgccggggg ccacgtcggc acgggctcgg tcggctggcc
    24361 gaacgtcccc acgggccggg ggtcggtggt cgtgccgccc cggggggcgt accactgggg
    24421 ggtggggttc tggtcggtca tggcgaacgc tccttcgacg ggggtgactg gcttgcaccc
    24481 agtcaatccc gccgacttgc gagcactctg atcagttgct gggagtgcgc tgtgcgacgg
    24541 ggcgccgctc ggcaggtgag ggccgctggg gcagtcggac gacgaaggtg gccccaccgc
    24601 ccggggtgcg ggcgaccccg acctggccct ggtgacgggc cacgatcgcg gcgacgatcg
    24661 ccaggcccag gccgcttccg ccaccagccg ttcgcccgcg cgagggatca gcccggtaga
    24721 accgttcgaa gaccttggtc gccacctcgg gggggacccc cggcccgtgg tcgcgcacct
    24781 cgagggtggc accgtgcgcg tcgagaccga cggcgacctc gacaggggtg cccgccgggg
    24841 tgtgccgcag cgcgttggcg acgaggttgg tgacgacctg gcgcagcttg ggctcggagc
    24901 cgacgacgac cgtcggtgcg accggcccac cgagaccgag gaggctgatc cgacgttcgg
    24961 gggcgcgcac gcgcgcgtcg gccacggcat ccgccgcgag gacggtgagg tcgacgggag
    25021 ccagctcgac ctcgggggcg tcgtcgatgc gggcgagcat gaggaggtcc tcgacgaggc
    25081 cgctcatccg ctcggactcg gcctcgatcc gttccatggc gccgccgacg gcttcggggt
    25141 cgcgcacggc gccctggcgg tagagctcgg cgtagccgcg gaccgtcgcc agcggggtgc
    25201 gcagctcgtg ggaggcgtcg gcgacgaact ggcgcatccg ctcctcgctg gcctctcgca
    25261 ccgcgaacga ggactcgatg cgcgcgagca tcgtgttgag gctgcgcgac agcgaggtca
    25321 cctcgtcgtc ggcctggcgg accgggatgc gttgcgtgag gtcgcccgct gcgatggccg
    25381 cggcggtgtc ctcgatgcgg gtgagcggcc ggaaggcgcg ccgcacggca taccgagcca
    25441 tcgccagcga ggcgagcagg acgatcgtgc tgatgagccc cgtcgtgatg acgagctggg
    25501 tcacggtgtc gttgacggtg tccaggggga cgccgacggc gaaggtggag tcctcgccga
    25561 cgacccggcc cgcgatgaag cgccactgcg ggcggccccc ctccgacggc acggtgaacg
    25621 gctcgccact ggtcacgcgc gggtcgtcga cggtcaggac gggaaggacc ggccgcgccg
    25681 tctccccggt gggcagccgg gtgatcgccc ctcgggcacc ttggaggtgg aatgcgtacc
    25741 ccgtcggcag cgccgacccg gtgctgcgca ggtcgtcgaa cacctggttg gccacgggtc
    25801 gcgcgaccga gcgcagttcc gaatcgaccc ggtcgagcag gtccgagcgc atgaggtagg
    25861 ccgtcgcgag gctcgtgagg atgagcgcgg ccccgacgag ggtcccgacg atcgccacga
    25921 ggcggcgccg cagcgggact gcctcgaggt ggcgcacgac acgggcgacc gggtcgtcct
    25981 tcgctgcggg cggggtggat gccggtgggt gggatgccgg ggggtcggat gccgggggag
    26041 tggtcgccgg ggaggcgctc gtcacggtca ggcctgcggt ggcaggcgca gcacgtagcc
    26101 gaccccccgc ttggtgtgga tgagcggggg taggccgtcg gcgtcgatct tgcgacgcag
    26161 gtaggagatg taggactcga cgatcccggc ctcgccgcgg aagtcgtagt cccagacgtg
    26221 gtcgaggatc tgcgacttcg acaggacccg gttcgggttg agcatgaggt agcgcagcag
    26281 cttgaactcg gtggggctgg tctcgatcac ccgaccggcg cgacgaacct cgtgcgagtc
    26341 ctcgtcgagt tcgaggtcct cgaaggtcag cacgaggctg tcctgctcgg cgtcgccccg
    26401 ggtgcggcgc agcacggccc ggatgcgggc gacgacctcc tcgaggctga acggcttcgt
    26461 gacgtagtcg tcgccgccga cggtcagtcc cttgatcttg tcgtcgacgg agtcgcgggc
    26521 agtgacgaac acgatgggca gctggcggcc ctgttcccgc aggcggcggg tgacggtgaa
    26581 cccgtccatg tcggggagca tgacgtcgag gacgcacagg tcggggtcgt gggcatcagc
    26641 cacgcgcagc gcctcggcgc cgctggccgc ggtggcgacg tcgaacccgg cgtagcgcag
    26701 cgacgtcgcg agcagttcgc ggatgttggg ctcgtcctcg acgacgagca ggcgggcctc
    26761 gggcgtcggg gtgctggtca cggcgtcagt ctggcgcgtg accctgtgag ttcgctggac
    26821 atcgcctgaa ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcggcgg
    26941 gtgagacttc cgctgccgca ggtcgagccc gacgcaaccc tgcccgagtc cgtgacgatc
    27001 tacgaggtcg ggccacgaga cggcctccag aacgaggcgg cggtggtgcc ggccgtggtg
    27061 aaggccgagt tcgtcaggcg cctggccagc gcgggtctgg ggacgatcga gaccacgtcg
    27121 ttcgtcgacc cgcggtgggt gccgcagctc gccgacgccg aggaggtgct cggtctgctc
    27181 gacgagcagg ggcgcggggc gcagcgaccc gtgctcgtgc ccaacgagcg tgggctggac
    27241 cgggcgctgg ctgctggtgt cggcgcggtc gcggtgttcg gcagcgccac ggagatgttc
    27301 gcgcgcaaga acctcaaccg cagcgtcgag gagtcgctgg ccatgttcgc gccggtcgtc
    27361 gagcgtgctc gggccgaggg gctgtgggtt cgcggctacg tctcgatgtg tttcggcgac
    27421 ccgtgggagg gccccgtgcc ggtggagcag gtcgtcgagg tgtgccggcg actgatggac
    27481 ctgggctgcg accagctctc gctcggcgac acgatcgggg tcggcacggt gggccatgtg
    27541 caccggctgc tggcggggtt ggatgccgtg ggcatcaccg ttgaccgcgt cggggtgcac
    27601 ttccacgaca cctacgggca ggcgctgtcg aacacgctgg cggcactgcg ggagggggtc
    27661 accgtcgtcg acgccgccgc gggggggctc gggggctgcc cgtacgccaa gtccgcgacg
    27721 ggcaacctcg ccacggagga cctcgtgtgg gcgctcgatg gcgctggggt acacaccggc
    27781 gtcgacctcg atgcactggt ggccaccagt gcgtggatgg ccggacacct cggccggccg
    27841 tcgccctcgc gggtcgtgcg tgccctcacc ggcgcctgac ctgctgaggt gtcccggtgc
    27901 acggcctcga ctgtgatgtg aactgcctgt gagtctgtgc tgaggtcggg actttcgctg
    27961 cctgcgggcg gatcatggcg gtatgagtga cccggccgcc cccgcccccc agcccggctc
    28021 tgcacctgtg agtccgcaag cccctgcgcc cgacccgggc tcgcacgggt ggttcgcccg
    28081 ggcggcgtcc acgaccgggg gccaggtcgc cctcgtgctg ggtgccgtgt tcacgaccct
    28141 gctgctggtg acgggggttg gtctggcgac cgcggcggtc gtcggcggcg tcctgtacgc
    28201 ccacggcgac gatgggcgga tgccggtgtc tgatcgtgga cccggtcagg gcaaggggct
    28261 ggcgctcggt cacgacaagc agcgggggat gggtcgcggg gacgaccagg gccagggccg
    28321 gggcgaggcc gagggcgcac cgggtcgcgg gaacggcaac ggcaagggtg gccccggcgc
    28381 cgggcagggt cagggcaacg gccgtggagc gcccggcatg ggcaacggca tggggatggg
    28441 gcggcagggc ctgggcgcgc cggacgtgct gcacggggag ttcacgacga acgtcaccgg
    28501 caccccgacc gtcatggtcg tgcagtcggg tcaggtcacg gcgtacaccg ccggcaagag
    28561 cctcacggtc aggagttcgg acggcttcga ggccacctac gccctggacg aggccgtcgc
    28621 ctcgatgcgt ggcgcggccg acctcgcggc gggagcccag gtgcaggtca tcgcagccaa
    28681 ggaggggatg aaggtcactc gcctgtcggt ggagtgaggc cgttgacctc gggggtgatg
    28741 cggcgctcga gcgccagcgc cagcagggcg acgaccgcga ggcaggcgat gagcaggccg
    28801 atgaacaccc cggcgagctc gcggtccagc agcaccccgg cgacgatggg cgccgtgatg
    28861 gccccgccct ggaacgcccc cgatgacacc gcgttgaacc gcccccggtc ccggtcgctc
    28921 gcgaggtcgt tgacgatggc gggcacggtc ggttgcagca tcgtctcgcc gaggccgaag
    28981 aagatgtgga acacgatgac cccggcagcc gctgcgagcg tgccggcgac gagcccggtg
    29041 gcgccgagga tgagccacgc cgccgcccac agcgccacca accccaggaa gacccgcgtt
    29101 cggcggcggc cgtcgatgat ccgcagcacg aggaactgca tgcccacgat cactgcggtg
    29161 ttcgccgcga acgccaggcc gatggtgcgc gtcgacacct cgctgacctc gcgggcgaag
    29221 gccgggaagc cagcctccat ctggccgtag ccgacgaagc tcgacaggaa cgtcagggcg
    29281 gtgatccaca cgaccgccgg attgcgcagg atcgtcaggt agctcacgcc cggcacggcg
    29341 gatgccgtgt cctgcgggcc gctgggcgcg ctggcctgcg gtggcccccc ggttcgtcgc
    29401 aacggtccga ggagcagggc gatggggatc agcatgcagg cagcgtcggc aaggaagatc
    29461 gccgtgaagg tcgcagggcg gtcgacgtcg gtgaggaagc cgctgagcac cccaccgatg
    29521 ccgatcccga ggttgaccag ggcgaagttg atgccgaaga actgggtgcg caggggaccg
    29581 gagacgatgc tcgaggtcag ggcgttgaag ccgggccagc cgatgccgaa gctgatgccg
    29641 atcagggtga acgcggcgac gaaggccggc accgaggtcg cgaaggccag gatcaccgcc
    29701 ccgaccagct gggcgaggtt gccccagatc aacatgacgc gagagcccag acggtcgatc
    29761 agggcaccga ccgggccggt cacgacgagt gcgaccccgg cgatgaggct catcagcagc
    29821 ccggccacat cgagctcgat gccgcgcacc tcgtgcacgt agatgatcgt gaacggcagg
    29881 gtcatccccc ggccgagcgt cgagatcgcg gtggtcgaga gcagccagcg accgggcgtg
    29941 gggagcgact cccagaagcc ccgcagggtg agacgcgagg cgttgggctc catggggcac
    30001 accgtgccat gacgcggtga cagcggcgac cgggtttcct gcgcgaccac acaaggcccc
    30061 gcgaaaccac gcgacgcaca cggcatccgg gcgttcgatc aacgcaggcg cgcgggaggg
    30121 tggggaccgg ttagccttga ggttgcgccc gctcgggcgt tctccgcgat ctggacaatg
    30181 aggtggaccg tgccgactgg caaggtgaag ttctacgacg ccgacaaggg tttcggcttc
    30241 gtcgccggcg actccggcga aggtgacgtg ttcgtcccct cgagcgcact gccccagggc
    30301 gtgaccggcc tcaagggcgg tcaacgcatc gagttcagca tcgccgaggg ccggcgcggc
    30361 gcccaggccc tgtccgtgcg gctgctcgac cccacgccct cggtggcgga caaccgtcgc
    30421 gtggccgggc gcaagcccgc cgacgagatg gtcgtcatcg tcgaggacgt catcaagctg
    30481 ctcgactcgg tctccgaggg gctgcgccgc ggccactacc ccgacaaggc ccacggcaac
    30541 aagatcgccc aggtgctgca cgcggtggcg ggcgacctcg agctgtgagc gccgcggtga
    30601 agtcggccgt gaagaccgac gcgacgctcg cggcggcggt cgagaccgcg cgcgaggcgg
    30661 ccgtgtcgat cgccgaggcg gatgccgtcg gcgcccacct cggcatggag atggtgcagg
    30721 accgggtcgc cactcactac ttcgagtgtg ccgccctggc ctaccgcggg tggcgctggg
    30781 cgatcaccgt tgcccgggtg ccccgctcga agaccgtgac ggtcagcgag accaacctcg
    30841 tgcccggcga ggagtcgctg ctgtctccgc agtggctgcc ctacgccgag cggctggccc
    30901 cgggtgacgt cggccccggt gacgtcaccc cgctcattgc cgatgacccg atgctcgttg
    30961 ccgggttcga ggccacgggc gacgacgacg tcgaccaggt cgcgatctgg gagctcggtc
    31021 tcggtcgtgc tcgtgtgctg tccgccgaag ggcgtgaggc ggcggcccgc cgctggtacg
    31081 agggggagtc ggggccgacg gcgccgattg ccgtcaaggc accggctccg tgttcgtcgt
    31141 gcgggttctt cgtgcccatg gccggtgccc tgcgctcggt cttcggtgtc tgcgcgagcg
    31201 agtggtcacc ctcggatggt cgcgtcgtca gcctggacca cggctgtggt gcgcactccg
    31261 aggtcgacat ggacgccccc gaacccgagc gggtcaccgc gccgatcctc gacgacttcg
    31321 agctcgaccc cgcctgacct gctcttggtc acgtggggtc gtcgactccc tgcgtacggt
    31381 tgacgtacca ccaggcaaag gcgccgagcc ccagcccgct gacacacgcc cacggccacc
    31441 atgagcgctc gcccgtgtgc agatccggga cgagcagggt gacgacgagg gcaaccgccc
    31501 agatcgcggt gccgacgagg acgatcgtgc gggtcggcac ggtgagcggg tcgaccggtg
    31561 cggactgccc gagcggggtc gcgcgctgct cgggagccgg gtccgtcggg gtcacccgcc
    31621 cactgtaacc gggcccgagt gtgccgagat cgaccaatag gtcgatgtgg gtacggggga
    31681 gggcgtcccc ggaatgacct aatggtcggt gtgggtacgg gtgggggtga ctggggtgta
    31741 cgccgagtga cgtattggtc gatctcggga cgtgggaatg gttagcacag gtaatgagtt
    31801 ggggtaatca actgcccgtc ctgccggagg agaccaccat gagccccatc accgctccgg
    31861 tcgcgccctc gtcgacgcag gccacccggc ccccggtgcc ccggccgatg acccgcgccc
    31921 agcgcgaccg gctcaccagc gacctgcgga tggcctgcat gcggatcgcc cgtcgcgtgc
    31981 gcctcgagag cgacgcactc gtggcgccgc accagttctc cgtgctcgtg cggctggagg
    32041 agcggcccag cacccccaag gacctcgcca ccatcgagaa ggtcagcgcc ccgagcatga
    32101 cccgcaccgt caatgccctc gtcgacctcg ggctcgtcgc ccgcgccgac gaccccaccg
    32161 acggtcgaca ggtcatcctg tcgctgaccc cggccggtcg cgagacgatc aaggaaaccc
    32221 gtcgcaggcg ccaccagtgg ttcgccagcc agctcgagac gctcaccgac gacgagcgcg
    32281 ccctgctcgt gcaggccacc gcgctgctcg aacgggtggc cgccgaatga gcccgacctt
    32341 cacctcgttc gaggtccgca actaccgcat ctggttcgcc ggtgccctcg tgtcgaacgt
    32401 cggcacgtgg atgggccggg tcggccagga ctggctcgtg ctcacggtgc tcaccgcggg
    32461 ctcggcgacc gccctcggcg tcgtcaccgg gctgcagttc ctgccgttcc tgctgctcgc
    32521 cccgtgggcc gggctgatcg ccgaccgctt ccccaagcgc cgcaccctct tgctgaccca
    32581 gaccatgctg gccgtctcga gtctgctgct cggggtgctc gccgtcaccg gtctggccca
    32641 gctctggcac gtctacgtga tcgccctgct ccagggcgtg acgacggcca tcgacaaccc
    32701 cgcccgtcag gccttcgtgt ccgagatggt gccgaacgac aagctcgcga atgccgtgtc
    32761 gctcaactcg gcatccttca acctcggtcg cctcatcggg ccgggcgtcg ccgggctcgt
    32821 catcgcggcg tggggcaccg gccaggcgct gctgctcaac accgtgacct tcgtgttcgt
    32881 catcgcggcc ctgctcctgc tccgggccaa cgagctcaag cccgcgccgc tcgcgaaggc
    32941 gcgtggcggc atccgggagg gtttctccta cgtccgcaac cggggcgacc tgcagctcgt
    33001 catgctgctc gtcttcgtgc tcggcacgtt cgggatgaac ttccagatca cgatggcgct
    33061 catggcgacc aaggtgttcg acaagggcgc tggcgagttc gggctgctcg gctcgatcat
    33121 ggccatcggc tccctgtccg ccgcactgtt ctcggcccgc cgcaaggtgc cgcggctgcg
    33181 ggtgctgctc atctccctcg cggcgttcac cgtggccgcg acggtggcgg cgatggcccc
    33241 cacctactgg acgttcgccc tggccctggt gcccgtcggg ttctcggcgc tcacggcgct
    33301 gacgacggcg aacgccatgg tccagacccg ggtcgcgccg gagatgcggg ggcgcgtcat
    33361 ggcgttgtac atggcgatct tcatgggcgg cacccccgcc ggtgccccgc tcatcgggtg
    33421 gatctccgag acgttcgggc cccgctggac gatcggtatc ggtgccgtgg cgatggggat
    33481 cacgctggtc ggtgtgtccg tgtggctcgc gaggtcgcag aatgtccgcg tgagctacga
    33541 gtcgcaacga cgcccgaggg tgcgcgtcgc cgtcaccagc ctgccgaccc cggcaacggc
    33601 gatcgccccg gtaccggagc cggctcgatg accccgctgt tccggcggcg gatgaccctc
    33661 ggtgtgctcg gcgtgctgat cgcagtggcg gcggtctacg ccctcacccg ctgacccacg
    33721 ctgcccccga cttcgtgcga ctttgctacc ggattcgggc caatcgcggc ccgcagccgc
    33781 actcaagtcg cacgaagtcg ggattggggg ggggtcaggc caggtgctcg acgacgtgct
    33841 cgatgcagcc gatgagcctg ctgacgtcgt cgggctcgac cgccgggaag caggccaccc
    33901 gcagctggtt gcggccgagc ttgcggtacg gctcgacgtc gacgaccccg ttggcgcgca
    33961 gcgtcttcgc gatggcggag gcgtcgatcg cgtcgtcgaa gtcgatcgtc gcgacgacct
    34021 gcgagcggtt cgcggggtcg gtgacgtacg gcgtggcgta gctgctcgcg tcggcccagt
    34081 cgtagagccg gcccgagctg tccttggtgc gggcgctcgc ccactccaga ccgccctgcc
    34141 cgttgagcca ctgcacctgg ttggcgagca tggcgagggt cgcgagcgcc ggggtgttgt
    34201 aggtctgctc gaggcggctg ttgctgatcg ccgtcggcag gctgaagaag tcgggcaccc
    34261 agcggccgga tgccgtgatc tcctccacgc gcgccagtgc ggccggcgag aacagcgcga
    34321 tccagaggcc gccgtcggag ccgaacgact tctgcggggc gaagtagtag acgtcgctct
    34381 gggccacgtc gacggggagc ccgccggcac ccgaggtggc gtcgatgagc acgagggcgt
    34441 cggcatccgc accctcgacc cggtgcacgg gagccatgac accggtgctg gtctcgttgt
    34501 gcgcccaggc atagacgtcg acgccggcct cggcgcgcgg ctgcgggcgg gtgcccggct
    34561 cgctggagat caccgtcggc tcaccgagga acggggcgtt cttcgtcacg gtggcgaact
    34621 tgctcgagaa ctcaccgaag gtgaggtgct gggcgcgctc gcgcacgagg ccgaacgcgg
    34681 cgacgtccca gaacgccgtc gcgccgccgt tgcccaggac gacctcgtag ccctcgggca
    34741 gcgagaagaa gtcagccagg cccgagcgga cctgggcgac gatgtcgcgg accggcttct
    34801 ggcggtgcga ggtgcccatg accgtggtgg cgatcgcgct gagggcctcg atctgggccg
    34861 ggcggacctt cgaggggccc gagccgaagc ggccgtcggc cggcagcagg tcagcgggga
    34921 tggtgatcgg ggcgtcgctc acgatggttc tccagggtcg gcgtggggtt gagtcgtggt
    34981 tgcggtcgtc cactcctgtg agggaacggg acacgatgac tgcgggccga cgtcgtcggc
    35041 gacccgtgac cggcatccat cttcacccac gatgcccgga tgccggacgt gagttccagc
    35101 acgtggaacc ccttgtccga cccggccaac cccggtcgac cccgcgggtc agcgctccgg
    35161 gtcgaacccg gcgacgtcct cgagcgggcg cgggcccggg ccgacgtagc gcgcgctcgg
    35221 ccggatgagc cgcccggtgc gcttctgctc gagcacgtgg gccgaccagc cggccgtgcg
    35281 ggctgccgcg aagagcgggg tcatcatcgt gccggggacg tcggcgaagt cgagcagcac
    35341 cgcggcccag tagtcgacgt tggtctccat gcgccgctcg gggtagcgct cggccagctc
    35401 ggcgatggcc gccttctcga gctcgagggc cacctcgtag cggggggcgc ccaggcgctg
    35461 acaggtgtcg cgcagcaccg cggcccgcgg gtcgtacgcc cggtagaccc ggtgcccgaa
    35521 gcccatgagg cgctccttgc ggtcgagcac ctccttgacg tagcggcgcg cgtcgcccat
    35581 ctgctcgacg ccctcgatca tgtgctgggc ccggctgggg gcgccgccgt ggaggggacc
    35641 cgagagggcg ccgatcgcgc ccgagaggca cgccgcggca tccgcgccgg tcgaggcgat
    35701 gacgcgggcg gtgaacgtcg aggcgttcat gccgtgctcg gccgcggaga tgaagtacgc
    35761 gtcgatggcc tcgacgtgct tggggtctgg ctcgccgcgc cagcggatca tgaagcgctc
    35821 ggcgatggtg cggcccttgt cgacctcgct ctgcggcacc atcgggctgc tctggccgtg
    35881 ggccgactgg ccgatgaacg agatggccat gaccgaggcc cgggccaggt tgtcgcgcac
    35941 ctcggcgtca tcgatgtcca ggagcggctt gaacccccac accggggcca gctgggccag
    36001 cgccgactgc acgtcgaccc ggatgtcgcc ggagtgcacc ggcagcggga acggctcggc
    36061 gggcggcagc cccgggtcga actcgttgtc gacgagcagc ccccagacct ggccgaagga
    36121 gacccggccg acgaggtcct tgaggtcgac gccgcggtag cgcagcgccc cgccctcctt
    36181 gtcgggctcg gcgatctcgg actcgaacgc gatgacgcct tcgagcccgg gacggaagtc
    36241 agggtcggtc gttgctgcag tgctcatggg gatccccaat cgtgtgccgg tcgccgttgt
    36301 cgggcggccc cattgtgcac gccgcgcgtc agttggcccc accggccgtc ccgtgggtgg
    36361 acatccgtcg gtccctgacg gccatggctg gagcgccgac gtcgcgcagc tcgaacaggt
    36421 cagtcgcttc gagcaacttg ctcagcttgg cgtagccgta gttgcgcggg tcgaacgagc
    36481 tctggttgga gatgcgctgc ccgaccgtgc tgacccgggc ccagccggcg tcgtcggcag
    36541 caccttcgac ggtgcggcgc aggagggcga cgagcttggc gtcgctgcgc agctggttgc
    36601 gcgtgagtcg ggcgggggtc tcaggggtgg cggcccgttc ggtcgccttc tccgcggcnn
    36661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ggccttctcc gcggctgcga
    36781 gggtggctgc cttcttgcgc gccgtcgtct tcttcgcggg cggcgcggcg gatgccgtgt
    36841 cgagggcgga tgccgtgtcg gcggcgttcg tcgtggcgcc ctccaccgtc gccgcccgtg
    36901 cggaggcgcg tcggcgcggc ggcgccgcgg gtgccgactc gggagcgacc ggtgccacgg
    36961 catccggcaa tgggctcgtg gctgcgaggc ggttgagcac gaggaagcgc gtgcaggcgc
    37021 tctggaaggg ctgtggcgtc ttggcgtccc cgaacccgta gaccttggcg ccgtgctcgc
    37081 gcaggtgcat gacgagcggg gtgaagtcgg cgtcggagga ggcgatggcg aaccagtcgg
    37141 gccgctgggt gtgcaggatc tcgaccgcgt cgacggccag ggcgatgtcg ctggcgttct
    37201 tgcccttgct ggggtcgaac tgctgcacgg ggcggatcgc gttgtcgtgc agcacctcct
    37261 gccacgactt caggccggtc ttggtccagt tgccgtacgc ccgccggatg ttggtctcgc
    37321 cgtggcggga gagctcgtcg aggatgacgt cgatgcgggc cgccgtcgcg ttgtcagcgt
    37381 cgatgagcag ggcgatctgg tcgggcttgt ccatgcccgg accttatgcc cgaggtggca
    37441 ggtggcgacg ggtcaggcat gatcggccgg tgctgcgctc ccacctgccg gacctgacgc
    37501 cgttgcgcga gaacgccgcg taccggcgcc tgtacgtggg gttcacgctg tccaacgtcg
    37561 ggtcgcagat ggcggtcgtg gcgatcgggc tgcaggtgta cgacatcacc gggtcaacgg
    37621 cctcggtcgg gctcgtgggg ctgttcgcgc tcgttccgct cgtcgtcatg ggcctgtacg
    37681 gcgggtccct gtcggaccac cacgaccggc gcaccgtggc gctggcggcc aacatcgtcg
    37741 cgtggctgac ctccatcgcg tgcgcggcgc aggcctggct cggcaacgag aacgtgtggg
    37801 tgctgtacgc gatcgtcgcg gtgtggaacg gggccttcgg ggtcagctca cctgcccgct
    37861 cgagcatcta cccgcggatc ctgccgcgcg agcagctgcc cgccgcgaac gcgctcgggg
    37921 tgttcgccat gtcgacggcg atgatggtcg ggccgctgct ggccggcctg ctcgtggact
    37981 ggggcggttt ccgcaccgcc tactcggtcg acgcgctcat cacgatggcg gcgctgtggg
    38041 ggttgtggcg gctgcagtcg ctgcc
//
LOCUS       DAOSOC010000007        36634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1182_length_36634_cov_9.093988, whole genome shotgun sequence.
ACCESSION   DAOSOC010000007 DAOSOC010000000
VERSION     DAOSOC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 36634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36634
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1182_length_36634_cov_9.093988"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..577)
                     /locus_tag="PL014_01225"
     CDS             complement(47..577)
                     /locus_tag="PL014_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT55742.1"
                     /translation="MTGVTSADDTGGVDGEPGVRWLSHEEQIAWRAYLRASRELTVAF
                     ERDLQDVGISLPEYELMSMLSEAPDAQMRMSTLASLIVQSRSRVTHTAARLERRGWVR
                     RTPAPDDGRGVLLRLTEGGRVALDSFAVVHIASVRRHLIDVLTAEQFATLGATMQIVR
                     DAYAADPTMRGAVTIA"
     gene            816..1361
                     /locus_tag="PL014_01230"
     CDS             816..1361
                     /locus_tag="PL014_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOT55743.1"
                     /translation="MSNARTTLDGLTPGTWSIDPTHAEVGFIARHLMVAKVRGKFTEF
                     AGTVEVAEQLADSVANVTIKTASVDTGTADRDAHLRSADFFDSETYPEMTFVSTSFDG
                     STLVGDLTIKGITKPVTLDVEFNGVATDPWGNDKAGFEAKGELNRTDWGLTWNAALEK
                     GGVLVSEKITLVIDVELGKQA"
     gene            1452..2012
                     /locus_tag="PL014_01235"
     CDS             1452..2012
                     /locus_tag="PL014_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOT55744.1"
                     /translation="MADAARTTYLLVDGENIDATLGTSILGRRPRPEERPRWERLLQF
                     ARDQWDQPAQGLFFLAANSELPMTFVQALLAIGYRPIPLSGAPGQKVVDIAIQRTLTE
                     LRTREADVVLVSNDGDFVEEVGGLLDGRRVGLVGFVEFRNHAFNQLVELGLETFDLEY
                     DVMAFNERLPRVRVIAIDEFDPAQFL"
     gene            complement(2028..3440)
                     /locus_tag="PL014_01240"
     CDS             complement(2028..3440)
                     /locus_tag="PL014_01240"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT55745.1"
                     /translation="MTRRKKVLLGGVAGLAVVLGGAGTAYAAHFQDRALPGSTVAGQS
                     VAGLSRDEVAAAVRERADDVEVTLEAGEATRTASLADLGYAVDVDATVDAVFANRTWS
                     TYATSLMSSREVDAVVTTDPAATDVVIADLVKDSGGIGVDATVKLAKDKKSFVVVPAV
                     AGKTVVESTFQDAVGAAARDLTSASTVVEFTEAVPDVTTDEAQAVADKANALAKTPVA
                     VSDGEDTHKASVARKVSWISIPVTDGALGEPTIKADKVKAWAAKLAKEAKVEPRAGLR
                     NVTQSGDVKTVVTKARDGKEVSNADALATAAVAALSGGKAYEGTFDYKTTKATWKERT
                     IADGAERLAYPAAPGEKWIDVNLSRHTMTAYVGAKAVYGPISMVDGQDEKPTVRGTFK
                     VYYKRELMTMRGNNADGTRYETPNVPWSTFFHEGFALHGAPWRSSFGYSASHGCVNLP
                     VGVAKWVYDFAPIGTPVVSH"
     gene            complement(3574..>4074)
                     /locus_tag="PL014_01245"
     CDS             complement(3574..>4074)
                     /locus_tag="PL014_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOT55746.1"
                     /translation="ATGAHVLGLSGMMDGQVAHVRAALDAAGHTDTVLLAYAAKYTSG
                     LYGPFREAVQSSLVGDRGAYQQDPANATEAVRELELDIAEGADIVMVKPAGPHLDVIA
                     AAAAMSPVPVAAYQISGEYSQIEAAAERGWIDRDRVMLESLTGIRRAGAQIVLTYYAL
                     EAAARL"
     assembly_gap    4075..4174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4175..4311)
                     /locus_tag="PL014_01250"
     CDS             complement(<4175..4311)
                     /locus_tag="PL014_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOT55747.1"
                     /translation="MGFPQSRPRRLRRTPAIRRLVAQTRLHPADLVLPVFVREGIEEP
                     VP"
     gene            complement(4311..5933)
                     /locus_tag="PL014_01255"
     CDS             complement(4311..5933)
                     /locus_tag="PL014_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional uroporphyrinogen-III
                     C-methyltransferase/uroporphyrinogen-III synthase"
                     /protein_id="HOT55748.1"
                     /translation="MSPTRSTSPSTPRTPAQVAFVGAGPGDPGLLTVRASELLAAADA
                     VVIDQVARHDVVERWSAPGTPVVDAGHGDHGENLTHASRAKLVVRAAKAHPGGLVVRL
                     MDGDPAVFNGLAEEATACVKAGVSFEVVPGVSSVTAVPSYAGVPLTSASSTGVHVLVA
                     GARGVDLTGALDPKVTVVVIGAPDKAAQTFDALIAAGRDGATPVAVTERGTSTDQRTV
                     TTTLSSAGATMADGRFPVLAVVGSTVTMRETLSWFESKPLFGWEVLVPRTKEQSASTL
                     ARLQRHGAQAKVVPTISVEPPRTPQQLERAVKGMVTGRYEWVGFTSVNAVRAIREKFD
                     EFGLDARSFAGLRIAAVGGVTAQALRDWGITPDLVPTGEQSALGLLEVWPAFDPDLDP
                     IARVFLPRADIATETLVAGLQDIGWEVDDVTAYRTVRAAPPPASVRDAIKNGSFDAVL
                     FTSSSTVRNLVGIAGKPHPTTVVACIGPATAKTAEELGLRVDVLAPVANAEALVDALA
                     DHGRSLALAAQEAGEVVLRPSQRRPGSRRRAT"
     gene            complement(5930..6904)
                     /gene="hemC"
                     /locus_tag="PL014_01260"
     CDS             complement(5930..6904)
                     /gene="hemC"
                     /locus_tag="PL014_01260"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOT55749.1"
                     /translation="MSMLRLGTRRSALATTQSSWVADQLRALGHEVELVEVSTEGDRD
                     QTTPLAQLGGTGVFVSALRAALVDGRVDLAVHSLKDLPTTPDPDTLVAAIPVREDPRD
                     ALVARGGLTLGELPAGSTVGTGSPRRRAQLEALGFGLVVTGLRGNVDTRMRQVTEGHL
                     DAVVLAAAGLRRLGRIDAVSELLDPIQMLPAPGQGALAVEVRGDDAHTASLVAVLDDH
                     DTRACTTAERALLAELEAGCSAPVGALAEVVEGFDGPELSLRAVVTSPDGTADLRRSL
                     VGDPATPADLGRRLAHLLLEDGAAQLMSDSPTTDHSSTTDHPATERAL"
     gene            complement(6901..8208)
                     /locus_tag="PL014_01265"
     CDS             complement(6901..8208)
                     /locus_tag="PL014_01265"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOT55750.1"
                     /translation="MSLVVLGLSHHGAPLSLLESVALDPDARTALEQAVLRSEHVTEA
                     VVVSTCNRTEVYAECLTFHGALADITAALADTCSVDRADLQPHLFVHYEDRGIAHVFN
                     VASGLDSMAVGEAQILGQVRRSLARAQRHGHVGPALNTVLQQALRVAKRVHTETGIDL
                     VSGSLVQAGLARAQAALGPIGDLSVLVVGAGGMGALAAATVARSGARTVVVANRNHDR
                     ARTVADRVGGTSRHLADLGAALVEADVVISSTGAPGQVITADDVRRAVAARSGRPQVY
                     IDLALPHDIDLAVADLPATTRVGLAELGEDLSDAGSAPQVAQAGDLVTAEVAAYLMAR
                     SAEAVAPTVTALRSHAGALVEEELHRLERRTPGLSDAERAEVRRAVERVVDKLLHTPT
                     VRVKELAQDGQGGSYARALNELFDLDPRDVSLVSAPPTLPEED"
     gene            complement(8208..8888)
                     /locus_tag="PL014_01270"
     CDS             complement(8208..8888)
                     /locus_tag="PL014_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOT55751.1"
                     /translation="MSDAATTRRGIPEASVARLPGYLRALTTLAGHGVISVSSEELAN
                     AAGVSSAMLRKDLSHLGSYGVRGVGYDVERLAAEITAALGLTREWPVAIVGMGNLGRA
                     LAAYRGFEERGFEVVALLDGDRRLLGEDVAGRPVRPMSDLPGLVADEGLAIGVIATPS
                     SGAQDACDRLVAAGVRSVLNFAPAVLAVPDGVVVRNVDLSTELQILAFHGQRVAAAAA
                     ATTSEVVA"
     gene            complement(9083..9355)
                     /locus_tag="PL014_01275"
     CDS             complement(9083..9355)
                     /locus_tag="PL014_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOT55752.1"
                     /translation="MDAAQPLPVRVTLIGRPGCHLCDTARETIERVSADLGVGWQEHS
                     IDDDPQLREAWAEQIPVTLVDGRQHDYWRVDETRLRAALAAPPAVR"
     gene            complement(9390..10886)
                     /locus_tag="PL014_01280"
     CDS             complement(9390..10886)
                     /locus_tag="PL014_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT55753.1"
                     /translation="MAARQGALAAGAARASGERARRRSTPLIGAEPVVPAGDTADAVA
                     SATKKPAAKQATPKKTTPKKPAAPKKPAAKKVARTPSDQLVDSGTAGQKVARTPSDQS
                     VDSGTGEQAARRPHRPAPKVRSAPSRRASRQAGERHARSAPAPVAEAPRAPRVRLNPR
                     EKASRDAARPHLRIAEPPLDPTVIEAPERTGLPLPDVGDALSAAVGLLRFGAQAAGIS
                     PEDIERRVAQVLAYIRRRIDGDYDVDDFGYDADFTENVFYPMLRPIYRHWFRVEVRGI
                     ENIPATGGALVVSNHSGTIAIDSLMVQLAIHDEHPQRRAMRALGADLVFQTPFLGTVA
                     RRSGSTLATNSDADRLFAQGELVGVFPEGFKGVGKPFSERYKLQRFGRGGFVAAALAA
                     QVPIIPCSVVGAEEIAPIIGNMGTVARLFGLPYAPITPTFPLFGPLGLIPLPSKWIIE
                     FGAPIPTDSQGPGAADDPMLVFDLTDQVRETIQQTLYTLLMQRRSVFF"
     gene            complement(10886..11935)
                     /locus_tag="PL014_01285"
     CDS             complement(10886..11935)
                     /locus_tag="PL014_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT55754.1"
                     /translation="MASVVLVTGVSRYLGGLLARRLSADPSVDRVVGVDVIPPAHDIS
                     GVEFVRADIRNPMIARVIESAGVDTVVHMNVIATPTFVGGRTPQKEINVIGTMQLLAA
                     CQKSTTVRRLVVKSSAAVYGSTPRDPAMFTEDMGAKRSPSGGFGKDSIEVEGYVRGFA
                     RRRPDTEILTLRFANIVGPRIRTSITDYFNLPVVPVPLGYDARMQFVHEDDAIDALVR
                     ATTGEASGIVNIAGDGVLTVLQAAAIVGRPVVPMPLQAAGVVGGFAKRLGLADFSPDQ
                     MSFLAFGRGMDTRRMRSVLGFTPRYTSREAFEDFARTVGPAVPAAAIVGDAVSGVAGT
                     ASQVVLGLLGAGRGR"
     gene            complement(12071..12169)
                     /locus_tag="PL014_01290"
     CDS             complement(12071..12169)
                     /locus_tag="PL014_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOT55755.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLRKTRHQRRNKK"
     gene            complement(12361..12561)
                     /locus_tag="PL014_01295"
     CDS             complement(12361..12561)
                     /locus_tag="PL014_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT55756.1"
                     /translation="MSTERQLAEVRFLTVAEVAAIMRVSKMTVYRMVHAGELPAIRVG
                     RSFRVPEDEVHAYLRTSFVDTA"
     gene            complement(12851..14041)
                     /locus_tag="PL014_01300"
     CDS             complement(12851..14041)
                     /locus_tag="PL014_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HOT55757.1"
                     /translation="MAHRTSVVWDDEFTAYNFGPSHPMAPVRLDLTTRLARALGVLDH
                     VDLLSPQAATDEVLATVHDPHYIAAVRAASADPMAADVHRGLGTEDDPAFPGMHEASA
                     RIAQGTLDLCRQVLTGETDHGVNFCGGMHHAMRTHASGFCIYNDIAVGIQWLLDNGAQ
                     RVAYVDIDVHHGDGVEKIFWNDPRVLTISVHENGQHLFPGTGWPSDIGGPSARGSAVN
                     VALPPGTGDAAWLRSIESVVPPLVRAFHPDVLVTQHGCDTHMHDPLAHLTITMDAQRR
                     AHETLHDLAHEAADGRWVALGGGGYELVDVVPRSWTHLTAIAAHVPVPAQARVPQEWL
                     DHVEATMGRSAPTRMGDLPEESLPIWVQPWSMGHNPESPIDRAIMATRQAVFSHHGLD
                     PWFD"
     gene            14120..14788
                     /locus_tag="PL014_01305"
     CDS             14120..14788
                     /locus_tag="PL014_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT55758.1"
                     /translation="MARGSGTENDVLVIGLGRFGAAAALELRRLGHKVTAIEKDGFLA
                     EKFLGRISHVIHGDASDVAVIRDAKAGRFPIAVVAIGSSVEASVLAAANLVDAGVADI
                     WGKALSPEHARILDRIGVHHIVRPEADTGRRVAHLMGGRMQDYIEFDDGFAIVKMRPP
                     SETVGFTLEQSAIRSKYGVTVVGVKAPGQDFTYAVPSTKVSAHDVLIVSGPTTLIERL
                     AARP"
     gene            complement(14793..15320)
                     /locus_tag="PL014_01310"
     CDS             complement(14793..15320)
                     /locus_tag="PL014_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT55759.1"
                     /translation="MSEITVRPLGADDWERYRSVRLTALQESPDAFVASHEAESAEPA
                     EFWRERMERSTRLLAEVDGEPVGIASVGSADDDQPETAQLFGLWVAPEARGTGVATAL
                     VRAGAQTAAAKGKRQLHYWVGTDNGRAVAFASGFGFRPTAERRPMRVVSSDDGEEELA
                     MTLPLSGDRGGIAQF"
     gene            complement(15376..15942)
                     /locus_tag="PL014_01315"
     CDS             complement(15376..15942)
                     /locus_tag="PL014_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT55760.1"
                     /translation="MDAPTPPTQPTAGGEVGSPTGAHGIRIDQVTAALLPTYRDVRIA
                     ALIDSPRAFWSTYEQARARTDDDWAAFLETGPRTWLAFDGARPVGTVGLWRGEGQPED
                     ESTLVGMWVASVVRGTDVAERLVRTALRAASDAGVRRVVLDVAHENVRACAFYSRIGF
                     RPTGEVNSMPWDPAVTEEQMVLDLTARS"
     gene            complement(15947..16747)
                     /gene="proC"
                     /locus_tag="PL014_01320"
     CDS             complement(15947..16747)
                     /gene="proC"
                     /locus_tag="PL014_01320"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOT55761.1"
                     /translation="MGMVAIFGAGVMGETLLSGLIRSGRPVDELVITERRADRAAELT
                     EKYGVRVLDNAEAADLADTLVLVVKPQDMGALLAEIHDHVAEGNLVVSLAAGITTSYL
                     ESRLPQGRPVVRVMPNTPALVDQGMAAVSAGAHCDTAHLAEAQALLAATGRVVEVPEK
                     LQDAVTAISGSGPAYIFYVVEAMIEAGVVLGMPRTTATELVVQTLFGAATMIKETGQH
                     PTVLREQVTSPGGTTAAALRTLDDHKVRAAFITAMEAAARRSAELAGE"
     gene            complement(16845..17795)
                     /locus_tag="PL014_01325"
     CDS             complement(16845..17795)
                     /locus_tag="PL014_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOT55762.1"
                     /translation="MDPSDLLRGAMLQISESDLVRKAVVTAPVSRAVVARYVAGESVP
                     DAVTVAGELRTTNRLATIDYLGEDTTDAVQAATTRDTYVHLLDLLAEADLTAYGAAEV
                     SVKLSAIGQALPADGDAIALENARAICEAAQAAGTTVTLDMEDHTTTDRTLATLHALR
                     EDFPWVGAVLQSYLHRTEADCRDLATAGSRVRLCKGAYKEPASVAYQDGDDVDLSYVR
                     CLKVLMAGDGYPMVASHDPRLIDIAAALAGHHGRAPDSYEFQMLLGIRMNEQKRIADR
                     GHQMRVYVPFGEDWYGYLVRRMAERPANVQFFLRSLATNG"
     gene            complement(17833..18573)
                     /locus_tag="PL014_01330"
     CDS             complement(17833..18573)
                     /locus_tag="PL014_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55763.1"
                     /translation="MNARITLATASRILRQLRRDHRTLALMLVVPCVLLGLLAWMYAD
                     APGEVFNRIGPALLGVFPFVVMFVVTSIATLRERTSGTLERLLTTPLAKGDLMLGYAL
                     AFGAVAVVQALVATGFAVWVCGLAIAGPIWLLVVIALLDALLGTALGLLASGFARTEF
                     QAVQFMPAFVLPQFLLCGLLLPRDQLPSVLRWISDVLPLSYAVDAMKDVTVLADPLAD
                     VLPSLAVVTGWIALALVLGSLTLRRRTP"
     gene            complement(18570..19346)
                     /locus_tag="PL014_01335"
     CDS             complement(18570..19346)
                     /locus_tag="PL014_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55764.1"
                     /translation="MTNKVEGDAIVVEHLRVVRGSREVLPDLDLRVPRGQVVGLLGPS
                     GGGKSTLMRAIVGVQVVASGRVLVLGEDAGSPSLRHRVGYVTQSPSVYGDLSVRENVD
                     YFRAVLGAPADAVDRAIVAVDLTSHAAARVDNLSGGQQARVSLACTLVGDPEVLVLDE
                     PTVGLDPVLRRELWAIFHRLADQGHTLFVSSHVMDEAVRCDRLVLLREGRVLSDSTLP
                     ELLEATGAADAEAAFLALIDAADAAHANAAAETAQPEDAA"
     gene            complement(19410..20192)
                     /locus_tag="PL014_01340"
     CDS             complement(19410..20192)
                     /locus_tag="PL014_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT55765.1"
                     /translation="MPAPLIGLSTSSVYPEGCAAAFELAARLGYDGVEIMVWTDPLTR
                     EAGALRALAELHGIRILSIHAPTLLVTQRVWGNDPWVKIDRSIDLAVEVGADTVVLHP
                     PFRWQREYAAGFIDGVALREHDTGMRLAVENMYPWRGPRSREVEAYLPHWDPVPQPYD
                     HVTLDLSHTATAGSDAVGMVHDLGPRLAHLHLADGVGSPRDEHLVPGRGTQPCGEVLE
                     LLAESGFGGHVVVEVGTRRASAAQRELDLAESLAFARLHLAT"
     gene            complement(20244..21170)
                     /locus_tag="PL014_01345"
     CDS             complement(20244..21170)
                     /locus_tag="PL014_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOT55766.1"
                     /translation="MRLGVIDVGSNTVHLLVVDAHPGAQPLPATKHKIDLRLSEHVTD
                     DGHIGDEGSDRLATFVQECLEVAEHQGVENLSGFVTSAIREAPNGHQVLLEVKERTGV
                     VLDVLSGADEARLTFLAARRWFGWSAGTLLVADIGGGSLELAMGMDEDPDVAVSLPLG
                     AGRVTRDLLPGDPPSADAVREARRLVRASIAAELRPLARAGAPDHAVGTSKTMRSLAR
                     ICGAAPSSEGMYAARVLPRALLTEKLPEIAAMTDAERADLPGVSASRAHQLLAGAIVA
                     EAAMDLLGLDELEICPWALREGIILRHLDRLS"
     gene            21292..22155
                     /locus_tag="PL014_01350"
                     /pseudo
     CDS             21292..22155
                     /locus_tag="PL014_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22503..23192
                     /locus_tag="PL014_01355"
     CDS             22503..23192
                     /locus_tag="PL014_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT55767.1"
                     /translation="MIRSPNIWDSPDVYELENLASDRAGVIDLALNAIHPLDGSVLLD
                     VGCGAGFHLPRFAARGARVIGLEPHLPLVELARARLAAGADGGPAASVIAGDAERMPL
                     RDSSVDVAHARWAYFFGAGCEPGLSELARVLRPGGTAFIVDNDVTRSTFGAWFSRAYP
                     AYDPAGVQRFWDRQGFTTDHLTIEWTFDTREDLDAVARIELPPAVADAVLATHDGLTV
                     DYAVALRWKRF"
     gene            23523..25220
                     /locus_tag="PL014_01360"
     CDS             23523..25220
                     /locus_tag="PL014_01360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOT55768.1"
                     /translation="MSRRSPRRKSSGSLPQLPPNGLRVIPLGGLGEIGRNMTVFEHQG
                     RLLIVDCGVLFPDESQPGVDVILPDFTWIRDRLDKVDAIVLTHGHEDHIGGVPYLLRE
                     RPDIPVVGSRLTLALLEAKLTEHKITPRSVVVAEGDRRPMGPFDLEFIAVNHSIPDGL
                     AVAIRTAAGLVLHTGDFKMDQFPLDGRVTDLRAFARLGEEGVDLFLVDSTNAEVPGFM
                     ISEGELAPAIDTAFRNTKGRVIVSSFASHVHRIQQVLDTAHSHGRKVAFVGRSMVRNM
                     GIARELGYLNIPDGLVVKDLKALDRLRPDQQCIVATGSQGEPMAALARMANRDHPVSI
                     AANDTVLMASSLIPGNENAIYRVINELTRWGANVVHKGNAKVHVSGHASAGELAYCYN
                     ILKPTNVLPVHGEWRHLQANAEIAIRTGAEKDRVLIAQDGSVIDLVDGRAKITGTVPA
                     GLVYVDSMTVGGATEETLHVRRTLAEEGVVTVVCIADPDTGKLTEDPDYLAHGFPPDL
                     LDFSGATPAIQNALTKAANDNIEDLEQIEDLVRRAVSNWAHRTHRRSPLVIPVIVDA"
     gene            complement(25332..26213)
                     /gene="nadC"
                     /locus_tag="PL014_01365"
     CDS             complement(25332..26213)
                     /gene="nadC"
                     /locus_tag="PL014_01365"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOT55769.1"
                     /translation="MSEHVFPLDDARLLVERALDEDLGDGTDVTTVATIPEEQESVSH
                     LVARADGVVAGLPVVALVVEAVDARLGTGVVWVDLHVTDGDAVVRGDHLATLRGSTRT
                     TLISERTILNVLSRTSGVATHTRRWADALAGSGAMVLDTRKTTPGIRALEKYAVRCGG
                     GTNKRMGLYDVAMIKDNHKLAAGGLTAAYDSIRAAFPHVDIQVEVTTTAEALEAVAAG
                     ARFLLCDNMSTDLLRTTVDAVRATGEYVEVEATGGLTLEVAAAYAATGVDYLSVGALT
                     HSSPILDIALDLDPDPS"
     gene            complement(26210..27889)
                     /locus_tag="PL014_01370"
     CDS             complement(26210..27889)
                     /locus_tag="PL014_01370"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOT55770.1"
                     /translation="MPSAPLTLPRRLTAPAPGWTTSADVIVVGSGIAGLTCALRLREQ
                     VDRVLLVTKTVLSAGSTQWAQGGIAAVLDPDDTAQEHLDDTLVAGVGLCDVEAVRALV
                     TEGPERVHELVALGAEFDRDAGGEIKLTREGGHHRDRIAHAGGDATGAEISRALIAAL
                     HRVLDDPGIEVIEHALVVDLLTAADGAVCGVTLHVIGEGQVDGVGAAAGRAVVLATGG
                     LGQIYASTTNPPVATGDGMAAALRAGARVTDLEFVQFHPTVLFLGEGSTGQQPLISEA
                     VRGEGAFLVDGDGARIMAGRHPLEDLAPRDVVARAILDVMRDQGVDHVWLDARHLGVS
                     FLEQRFPSIVARCRELGFDPAVDLLPVAPAQHYASGGVETDLLGRTSLDGLYACGECS
                     CTGVHGANRLASNSLLEGLVFAHRIADDIAARFSSGDLPQREVGSTPGEDAVLDASRR
                     IEVQRAMTLGSGPVRSGQSTAGAQAELAALAAQPTTEAEPGPLTWETTNLLHLGQVLS
                     RVAHLREETRGGHVRSDFPERDDARWLTHLTATRRADGQVVITERPAGDPR"
     gene            complement(27902..28822)
                     /gene="panC"
                     /locus_tag="PL014_01375"
     CDS             complement(27902..28822)
                     /gene="panC"
                     /locus_tag="PL014_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOT55771.1"
                     /translation="MSDQQSAAPVVAHTREEFKAAREALSSRDVAVVMTMGALHDGHA
                     RLIQAARQRHDHVVVTIFLNPLQFGPKEDLSRYPRTFDADMEICRAAGVDVVFAPTPD
                     VVYPDGDPGVRVAAGPLGEVLEGASRPGHFDGVLTVVGKLLHITAARSAYFGQKDAQQ
                     LLLIRRMVRDLDFPVDVVSVPTVREADGLAMSSRNMYLTESDREVALCLSGALRAGAD
                     AAAFGPSAIRRAAREVLVEEPLALVSYLVLVHPHTLADVPEWYRGEALLAVAARVGTT
                     RLIDNTPVLVGPGGGALDVFSDVPHARADV"
     gene            complement(28819..29703)
                     /locus_tag="PL014_01380"
     CDS             complement(28819..29703)
                     /locus_tag="PL014_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOT55772.1"
                     /translation="MEHPARLTVGVVGAGRVGSVLGAALARAGHRVTGTYAVSEASRA
                     RAAELLPGVPVKDVAAVVAGAELVLLAVPDDALGHLVAGLAATGVWQAGQIVVHTSGR
                     HGVGILDPARAQHVLPLALHPAMTFTGTAMDLDRLVDCAFGVTADDALRPMAEALVLE
                     MGGEPVWIAETDRTAYHLALAHGANHLVTLTSEAMQILASAGVEDPQRVLGPLMRAAL
                     DNALRLGDAALTGPVARGDVGTVEDHLRELDRLSPEIRRTYVAMARATALRALDSGRL
                     RPASAEPLLETLADRTQP"
     gene            29949..30923
                     /locus_tag="PL014_01385"
     CDS             29949..30923
                     /locus_tag="PL014_01385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT55773.1"
                     /translation="MVPWRQAWHDALYGESGFYRRTEGPAGHFTTSTHGPLGVVLAEA
                     IGRLADREGCMHVVDLGCGRGELLTHLHTARPDLTLTGVDVVGRPNGIPDAVAWLRSP
                     GGGSLPEGLRGLVDVLVVAHEWLDVVPCTVAQVVAPGRLAVVLVDPVTGEEGLGGPLA
                     ADDLAWCARHWPVDDLPVGARVEVGGTRDAAWSDLVSRVGRGTLLAVDYGHGRGERPG
                     EGTLTGYRAGAQVAPVPDVSCDVTAHVAVDSLDHDELTTQREALRALGVTAALPEHGL
                     ARTDPVGYVAALSRSSAAAALMDPNGLGAFRWVLRRCPPAGARPSTAD"
     gene            30992..32125
                     /locus_tag="PL014_01390"
     CDS             30992..32125
                     /locus_tag="PL014_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOT55774.1"
                     /translation="MGAGALATADMVLNIGPQHPATHGVLRLRVVLDGERIVSAEPII
                     GYMHRGAEKLFEVRDYRQITVLANRHDWLSAFSNELGVVLGVEAMLGMEVPERAVWLR
                     TLLAELNRVLSHLMFLGSYPLELGAITPVFYAFREREELQAVMEEASGGRMHYMFNRV
                     GGLKEDVPAGWLGRVSDAVAAVRRRLPDLESLIVGNEILEARTRGVGVLDVGTCTAYG
                     VSGPIARASGLDVDLRRDAPYLAYGELFAPGGPGRVVTRTAGDCLARLEVLLEQVHVS
                     LDLAEACIDTLRSLPAGPVNQRLPKVLKVPEGERYTATENPLGFNGYYLVSRGEKTPW
                     RLKLRSASFNNVQVLSQVLPGCLVADMVAVLGSMFFVVGDVDK"
     assembly_gap    32184..32283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32284..33014)
                     /gene="folB"
                     /locus_tag="PL014_01395"
     CDS             complement(<32284..33014)
                     /gene="folB"
                     /locus_tag="PL014_01395"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOT55775.1"
                     /translation="MSDRIVLSGVRGRGFHGVFEHERREGQEFVVDVALTVDLAAAGA
                     SDDLVDTVNYGEIGAAALARIEGEPFDLIERLAEVIAGDALSHSAVDEVTVTVHKPQA
                     PVGVPFGDVTVSVTRQRHPVPVVIALGANLVRGEDSPADTLAAAVAELEFDVGLTGIR
                     VSPTYDTTPVGGPPGQPDYANAVLVARTTLSPASLLRALHAVEAGFDRTREVRWGART
                     LDLDLVQYGDPASGTDVVSERAHLTL"
     gene            complement(33007..33801)
                     /gene="folP"
                     /locus_tag="PL014_01400"
     CDS             complement(33007..33801)
                     /gene="folP"
                     /locus_tag="PL014_01400"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT55776.1"
                     /translation="MGVVNVTPDSFSDGGAWFTPQDAVAHGRDLVVAGADLLDVGGES
                     TRPGAQRPSAAEELRRVLPVIEALREVAPVSVDTMRAEVAAAALDAGAVVVNDVSGGL
                     ADPEMGALVAERGVPFIAMHWRAHSTVMQSFAVYDDVVADVSRELMARVEALTGAGVA
                     AENIVLDPGFGFAKKAEHNWELLRRLDEVMALGHRLLVGTSRKTFLGHVGRTPDAVRP
                     PLDRDVATAVTTAHVARLGVWAVRVHDVVSTIDTLDVVDALRGAHE"
     gene            complement(33871..34488)
                     /gene="folE"
                     /locus_tag="PL014_01405"
     CDS             complement(33871..34488)
                     /gene="folE"
                     /locus_tag="PL014_01405"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOT55777.1"
                     /translation="MSTPEVGADASGVGPRGVDLARAEAAVRELLFAIGEDPDREGLR
                     ETPGRVARAARELYAGLNQDPADVLSTTFSIDHEELIIVRDIPMYSTCEHHLLPFHGV
                     AHIGYIPAEDGKVTGLSKLGRLVEVFARRPQVQEQITTQVADALVTHLGAQGVIVVIE
                     AEHLCMSMRGVQKPGSRTITSAVRGQLRDPATRSEAMSLLLGGKR"
     gene            complement(34475..36580)
                     /gene="ftsH"
                     /locus_tag="PL014_01410"
     CDS             complement(34475..36580)
                     /gene="ftsH"
                     /locus_tag="PL014_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT55778.1"
                     /translation="MDFKRIFKAPLFWVLAVVVVMLTVFSFDGDGGYTTITTAQAEKL
                     IVDKKVDKAQMTTENVLTLDLRDGQTFSDEANNVSGATKVRAEFVDARAEQLVDSLRT
                     NLPNGYNDKVESTSAFWTIFISFFPVLLLVGLFWFLMSQAQGGGSRVMQFGKSKAKLA
                     TKDTPKVTFADVAGADEAVEELHEIKEFLAEPAKFLAVGAKIPKGVLLYGPPGTGKTL
                     LARAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKANAPAIVFVDEIDAVGR
                     HRGAGLGGGHDEREQTLNQLLVEMDGFDVKTNVILIAATNRPDILDPALLRPGRFDRQ
                     IAVEAPDMIGRHRILEVHSQGKPMASGVDLLAVARRTPGFTGADLANVLNEAALLTAR
                     GDKKLIDDEVLDEAIDRVIAGPQKRTRIMSAKERKITAYHEGGHALVAAAMNHTSPVT
                     KVTILPRGRALGYTMVMPTDDKYSTTRNEILDQLAYALGGRVAEEIIFHDPTTGAAND
                     IEKATGMARKMVTEYGMSERVGAIKLGQSQGEVFLGRDMGHQRDYSEQVAAVVDEEVR
                     RLVDDAHDEAWHAVNDNRDILDRLVLDLLDKETLNAAELAEIFKDVRKRPVRDLWLSS
                     EHRTLSDRPPVLTPAEQAALNGRSNGSGAPAGASVNPGDPGTEADIDAAARAGAPTQV
                     EDTPPTQIIQVPDGGRVDS"
BASE COUNT         5256 a        12852 c        13224 g         5102 t
ORIGIN      
        1 gttgctcggc ctcggccagg gtcgggcgag gtctcggacc gacgggtcag gcgatggtga
       61 cggcgccgcg catggtcggg tcggcggcgt aggcgtcgcg cacgatctgc atcgtggcac
      121 cgagggtggc gaactgctcg gcagtcagca cgtcgatgag atggcgccgg acgctcgcga
      181 tgtggacgac ggcgaaggag tcgagagcga cccgcccgcc ctcggtgagg cgcagcagca
      241 ccccacggcc gtcgtcgggg gccggcgttc ggcgcaccca gccgcgacgc tcgagccggg
      301 cggcggtgtg ggtcacgcgg ctgcgggact gcacgatgag ggacgccagc gtcgacatcc
      361 gcatctgcgc gtcgggtgcc tcggagagca tcgacatcag ctcgtactcg ggcaggctga
      421 tcccgacgtc ctggaggtca cgctcgaacg ccaccgtgag ctcacgagag gcgcgcaggt
      481 aggcgcgcca ggcgatctgc tcctcgtggc tgagccaccg gaccccaggc tcgccgtcga
      541 caccacccgt gtcgtcggct gacgtcactc ctgtcatcac tgacccactc atcgcgcctc
      601 cccggttcac cgaactcgcc catcgtggca cgggactgca gatacccgtg ggtgctccga
      661 tccatgtggc catctgcaca gacgtccctc ctgagaacga cctgagatgc acgtccatca
      721 cagcgtctca catggcgaga cacgggaatg gatgccgcgg ccggtttggt tgaagggaca
      781 actagcgagt gctaggccac gacaaaggag acttcatgag caacgcgcgc accaccctgg
      841 acggcctcac ccccggcacc tggagcatcg accccaccca cgccgaggtg ggtttcatcg
      901 cccgccacct catggtggcc aaggtccgcg gcaagttcac cgagttcgcc ggcaccgtcg
      961 aggtggccga gcagctcgct gacagcgtcg cgaacgtgac catcaagacg gcatccgtcg
     1021 acacgggcac cgccgaccgc gacgcccacc tgcgcagcgc cgacttcttc gactccgaga
     1081 cctacccgga gatgaccttc gtcagcacct cgttcgatgg ttccaccctc gtcggggacc
     1141 tgacgatcaa gggcatcacc aagcccgtga ccctcgacgt cgagttcaac ggtgtcgcga
     1201 ccgacccgtg gggcaacgac aaggccggct tcgaggccaa gggcgagctc aaccgcaccg
     1261 actggggcct gacctggaac gcggccctcg agaagggtgg cgtgctcgtc tccgagaaga
     1321 tcacgctcgt catcgacgtg gagctcggca agcaggcctg agccacaccc gactcatggc
     1381 gaacggccca cctcggacga ggtgggccgt tcgccatgag tcgcccgtga tgctgcgtct
     1441 agggtgagcc catggctgac gcagcgcgca ccacctacct gctcgtcgac ggcgagaaca
     1501 tcgacgccac gctgggcacc tcgatcctcg gccgccgacc gcgcccggag gagcgtcccc
     1561 ggtgggagcg gctgctgcag ttcgcgcgcg atcagtggga ccagcctgct cagggcctgt
     1621 tcttcctcgc ggccaactcc gagctgccca tgacgttcgt gcaggccctg ctcgccatcg
     1681 gctaccgccc catcccgctg tcaggcgcac cggggcagaa ggtcgtcgac atcgccatcc
     1741 agcgcacgct gaccgagctg cgcacgcgcg aggccgacgt cgtcctcgtc agcaacgacg
     1801 gtgacttcgt cgaggaggtc ggcgggctgc tcgacgggcg ccgcgtgggc ctcgtcggat
     1861 tcgtggagtt ccgcaaccac gcgttcaacc agctcgtcga gctcgggctg gagaccttcg
     1921 acctcgagta cgacgtcatg gccttcaacg agcggctccc gcgcgtgcgg gtcatcgcca
     1981 tcgacgagtt cgacccggcc cagttcctct gaccggccct gccggggtca gtgggagacg
     2041 accggggtgc cgatgggggc gaagtcgtag acccacttcg cgaccccgac cggcaggttg
     2101 acgcagccgt gcgacgcgga gtagccgaac gatgaccgcc agggcgctcc gtgcagggcg
     2161 aaaccttcgt ggaagaaggt gctccacggc acgttgggcg tctcgtaacg ggtgccgtcg
     2221 gcgttgtttc cgcgcatcgt catcagttca cgcttgtagt agaccttgaa cgtgccccgc
     2281 acggtgggct tctcgtcctg tccgtcgacc atggagatcg ggccgtagac ggccttcgcg
     2341 ccgacgtagg cggtcatcgt gtgccggctg aggttgacgt cgatccactt ctcgcccggg
     2401 gccgcggggt acgcgaggcg ctcggcgccg tccgcgatgg tccgctcctt ccacgtggcc
     2461 ttggtggtct tgtagtcgaa ggtgccctcg taggccttgc ccccggacag ggcggccacc
     2521 gcagccgtgg cgagcgcatc ggcgttggag acctccttgc cgtcgcgagc cttggtcacc
     2581 acggtcttga cgtcaccgga ctgggtcacg ttgcgcagac ccgcgcgtgg ctcgaccttg
     2641 gcctccttgg ccagcttggc cgcccacgcc ttgaccttgt cggccttgat cgtcggctca
     2701 cccagcgccc cgtcggtgac cgggatgctg atccacgaga ccttgcgcgc cacggacgcc
     2761 ttgtgcgtgt cctcaccgtc ggagaccgcc acgggggtct tggccagcgc attggccttg
     2821 tccgccacgg cctgcgcctc atcggtggtc acgtcgggca cggcctcggt gaactcgacg
     2881 acggtgctcg ccgaggtgag gtcacgcgcc gccgctccca cggcatcctg gaaggtgctc
     2941 tcgacgacgg tcttgccggc cacggccggg acgacgacga acgacttctt gtccttggcg
     3001 agcttcaccg tggcgtcgac gccgatgcca ccggagtcct tgaccaggtc ggcgatgacc
     3061 acgtcggtcg cagcggggtc ggtggtcacg acggcgtcga cctcgcggct ggacatcagg
     3121 gacgtggcgt acgtcgacca ggtgcggttg gcgaagacgg cgtcgaccgt cgcgtcgacg
     3181 tcgaccgcgt agcccaggtc ggccagggat gccgtgcggg tcgcctcgcc ggcctccagc
     3241 gtcacctcga cgtcgtcggc gcgctcacgc accgcggccg ccacctcatc gcgcgagagc
     3301 ccggccacgg actggcccgc caccgtgctg cccgggaggg cgcggtcctg gaagtgggcg
     3361 gcatacgccg tgcctgcacc cccgagcacc accgcgagcc ccgccacgcc gccgagcagc
     3421 accttcttac gcctggtcat gctcgtttcg ctccgcatct gcatcccgcc ggcgcacgaa
     3481 atgcgcttat cacaccacca tgatggtgca cgaacccggg ttcgcccaat ccgccgatcg
     3541 gtcgacgcac ggcgcgcgac cacggcgcct gcgtcacagg cgggcagcag cctcgagggc
     3601 gtagtaggtg agcacgatct gggcgccggc ccgtcggatg cccgtgagcg attccagcat
     3661 gacgcggtcg cgatcgatcc aaccccgttc ggcagcggcc tcgatctgcg agtactcccc
     3721 cgagatctgg tacgcggcaa ccggaaccgg cgacatcgcg gcggcagcgg cgatgacgtc
     3781 gaggtgcggg ccggccggct tgaccatgac gatgtcggcg ccttcggcga tgtccagctc
     3841 gagctcacgc acggcctcgg tcgcgttggc cgggtcctgc tggtaggccc cccggtcacc
     3901 gacgagcgag gactgcacgg cctcgcggaa cggcccgtag agcccggagg tgtacttcgc
     3961 ggcgtacgcc aggagcacgg tgtcggtgtg ccccgcggca tccagcgccg cacgcacgtg
     4021 cgccacctgg ccgtccatca tccccgagag cccgaggacg tgggccccgg tcgcnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcacc ggctcctcga tgccctcgcg
     4201 cacgaagacg gggagcacca ggtcggccgg gtgcagcctc gtctgcgcca ccaggcgccg
     4261 gatcgcgggg gtgcgccgca accggcgtgg cctgctctgc gggaaaccca tcacgtggcg
     4321 cggcggcgcg aacccggacg acgctggctc gggcgcagga cgacctcacc ggcctcctgg
     4381 gccgccaggg ccaacgagcg gccgtgatcg gccagggcgt cgacgagcgc ctcggcgttg
     4441 gcgaccgggg cgagcacgtc gacccgcagg ccgagctcct ccgcggtctt ggccgtggcc
     4501 gggccgatgc acgcgacgac cgtggtcggg tgcggcttgc ccgcgatgcc gacgaggttg
     4561 cgcacggtcg agctcgacgt gaacagcacg gcgtcgaagg agccgttctt gatggcgtcg
     4621 cgcaccgagg ccggcggcgg ggccgcgcgc accgtgcggt aggccgtgac gtcgtcgacc
     4681 tcccacccga tgtcctgcag gcccgcgacg agcgtctcgg tcgcgatgtc ggcacgcggc
     4741 aggaacaccc gggcgatcgg gtcgaggtcg gggtcgaagg cgggccagac ctcgagcagg
     4801 ccgagcgccg actgctcacc ggtcggcacg aggtcggggg tgatccccca gtcgcgcagg
     4861 gcctgcgcgg tgacgccccc gaccgcggcg atgcgcagcc ccgcgaacga gcgagcgtcg
     4921 aggccgaact cgtcgaactt ctcacggatc gcccgcacgg cgttgacgga cgtgaagccc
     4981 acccactcgt agcgcccggt gaccatgccc ttgacggcgc gctccagctg ctgcggcgtg
     5041 cgcgggggct ccaccgagat cgtcggcacg accttggcct gcgccccgtg gcgctggagg
     5101 cgggccaacg tcgaggccga ctgctccttc gtgcggggca ccagcacctc ccacccgaac
     5161 agcggcttgc tctcgaacca cgacaacgtc tcgcgcatgg tcacagtgga ccccacgacg
     5221 gccagcacgg ggaagcgccc gtcggccatc gtcgcgccgg ccgaggacag cgtcgtcgtc
     5281 acggtgcgct ggtcggtgct cgtgccgcgc tcggtgacgg ccacgggagt cgcaccgtcg
     5341 cgaccggcgg cgatgagggc gtcgaaggtc tgggcggcct tgtccggcgc cccgatgacg
     5401 acgacggtga ccttggggtc gagcgcgccc gtgaggtcga ccccgcgagc cccggcgacg
     5461 aggacgtgca ccccggtgga gctggccgac gtcagcggca ccccggcgta cgagggaact
     5521 gccgtgaccg agctgacgcc cggaaccacc tcgaaggaca cccctgcctt cacgcaggcg
     5581 gtggcctcct cggcgagccc gttgaagacc gcggggtcac cgtccatgag gcgcacgacg
     5641 aggccgcccg ggtgcgcctt cgcggcccgc acgacgagct tggctcgcga cgcgtgggtg
     5701 aggttctcac cgtggtcgcc gtggccggca tccacgacgg gggtgccggg tgcgctccac
     5761 cgctcgacga cgtcgtgccg cgccacctgg tcgatgacga cggcatccgc agcggcgagc
     5821 agctcgctgg cgcgcacggt gagcagcccg gggtcgccgg gtccggcgcc gacgaaggcc
     5881 acctgggccg gcgtgcgggg ggtggaaggg gaggtcgaac gcgtggggct cacagggcac
     5941 gctccgtggc ggggtggtcg gtcgtggagg agtggtcggt cgtggggctg tcggacatca
     6001 gctgggctgc gccgtcctcg aggagcaggt gcgcgaggcg cctgccgagg tcggcggggg
     6061 tggcggggtc cccgacgagc gagcggcgaa ggtcagccgt cccgtcgggc gaggtgacga
     6121 cggctcgcag ggagagctca gggccgtcga agccttcgac cacctcggcg agtgcgccga
     6181 cgggggcgga gcacccggcc tcgagctcgg cgagcaacgc ccgctcggcg gtggtgcagg
     6241 cgcgggtgtc gtggtcgtcg agcacggcga cgagggatgc cgtgtgcgcg tcgtccccgc
     6301 ggacctccac cgcgagcgcc ccctggccgg gggccgggag catctggatc gggtcgagca
     6361 gctcgctgac ggcgtcgatc cgtccgaggc gacgcaggcc tgcggcggcg agcacgacgg
     6421 cgtcgaggtg tccctcggtg acctgccgca tccgggtgtc gacgttgccg cgcagtccgg
     6481 tgacgacgag gccgaagccc agggcctcga gctgggcacg gcggcggggg gagcccgtgc
     6541 ccacggtcga gcccgcaggc agctcaccga gcgtgagccc gccccgggcg acgagggcgt
     6601 cgcgggggtc ctcgcgcacc gggatcgcgg cgacgagcgt gtccgggtcg ggtgtcgtgg
     6661 gcaggtcctt cagcgagtgc acggccaggt cgacccgccc gtcgaccagg gcggcgcgca
     6721 gggcggagac gaagacgccg gtgccgccga gctgggccag cggggtggtc tggtcgcgat
     6781 cgccctcggt gctgacctcg acgagctcga cctcgtggcc gagcgcacgc agctggtcgg
     6841 ccacccacga cgactgggtc gtcgcgaggg cggatcggcg ggttccgagg cggagcatgc
     6901 tcagtcctcc tcgggcaggg tcgggggtgc ggacaccagg gagacgtcgc gggggtcgag
     6961 gtcgaacagc tcgttgagcg cccgcgcgta gctgccgccc tggccgtcct gggcgagttc
     7021 cttcacgcgc accgtcgggg tgtggagcag cttgtcgacg acacgctcga cggcccggcg
     7081 gacctcggcc cgctctgcgt cgctcaggcc gggtgtgcgt cgctcgaggc ggtgaagctc
     7141 ctcctcgacg agcgcgccgg cgtggctgcg cagcgcggtc acggtcggcg cgacggcctc
     7201 ggccgatcgg gccatgaggt acgccgcgac ctctgccgtc acgaggtcac ccgcctgcgc
     7261 gacctggggt gccgacccgg catccgagag gtcctcgccg agctcggcga ggccgacccg
     7321 ggtcgtggcg ggcaggtcgg cgacggcgag gtcgatgtcg tgcggcagcg cgaggtcgat
     7381 gtagacctgc gggcgtccgc tgcgggcagc gaccgcacga cggacgtcgt cggcggtgat
     7441 gacctgcccc ggagcgccgg tggaggagat gacgacatcg gcctcgacga gggcagcacc
     7501 gaggtccgcg aggtggcgcg aggtgccgcc cacccgatcg gccaccgtgc gcgcccggtc
     7561 gtggttgcgg ttcgccacga cgacggtgcg ggcccccgaa cgggccacgg tcgccgccgc
     7621 gagggcgccc atgccgccgg cgccgacgac gagcacggac aggtcgccga tcggtccgag
     7681 ggcggcctgc gcccgggcca gcccggcctg cacgagcgag ccgctcacga ggtcgatgcc
     7741 ggtctcggtg tgcacgcgct tcgcgacccg cagggcctgc tggagcacgg tgttcagggc
     7801 tgggccgacg tgaccgtgac gctgggcgcg agcgagcgag cggcgcacct ggccgaggat
     7861 ctgggcctca ccgacggcca tcgagtccag gcccgaggcg acgttgaaca cgtgggcgat
     7921 gccgcggtcc tcgtagtgca cgaacaggtg cggttgcaga tcggcccggt cgaccgaaca
     7981 ggtgtcggcc agcgccgccg tgatgtcggc cagggcgccg tggaaggtca ggcactcggc
     8041 gtagacctcg gtgcggttgc acgtggacac gacgacggct tcggtgacgt gctccgagcg
     8101 cagcaccgcc tgctcgaggg cggtgcgggc gtcggggtcc agggcgaccg actccaggag
     8161 tgacaggggt gcaccgtggt gcgacaaccc gaggacgacc aggctcatca ggccaccacc
     8221 tcggaggtcg tggcggccgc ggccgcggcg acccgctgac cgtggaaggc gaggatctgc
     8281 agctccgtgg acaggtcgac gttgcgcacg acgaccccgt cggggacggc gagcacggca
     8341 ggggcgaagt tcagcaccga gcggaccccc gccgcgacga ggcggtcaca cgcgtcctgg
     8401 gcccctgacg acggcgtggc gatgacgccg atcgcgaggc cctcgtcggc gacgagaccc
     8461 ggcaggtcgg acatggggcg caccggccgc ccggcgacgt cctcgcccag cagccgacgg
     8521 tcaccgtcga gcagtgcgac gacctcgaac ccgcgctcct cgaacccgcg gtaggcggcg
     8581 agcgcccgac cgaggttgcc catgccgacg atcgcaaccg gccactcgcg ggtcaggccc
     8641 agtgcggcgg tgatctcggc ggccaggcgc tcgacgtcgt agccgacccc gcgcaccccg
     8701 taggagccga ggtgcgacag gtccttgcgc agcatcgccg agctcacccc ggcggcgttc
     8761 gcgagctcct cggagctcac cgagatcacg ccgtggcccg cgagggtcgt gagcgcccgc
     8821 aggtaccccg ggagtcgggc caccgaggcc tccgggatgc cccggcgggt ggtcgctgcg
     8881 tcggacaccg tcgctcaccg actcctttcg cgggcccgca cggccggatc ggagcgggcg
     8941 ggcgcacgcg ttcagcggcc cggccagcgg gtgagaccgt cagcgtacgg tcttgtgaac
     9001 gcctgcacaa agtcgtccga ggaccccgtg accctgctca ctcccccgtg tgctaaaagc
     9061 ttcgacagca gtccggatgc cgtcagcgca ccgcgggcgg cgcggccagg gccgctcgca
     9121 gacgggtctc gtcgacccgc cagtagtcgt gctgacgacc gtcgaccagg gtcaccggaa
     9181 tctgctcggc ccacgcctca cgcaactgcg ggtcgtcatc gatggagtgc tcctgccacc
     9241 cgaccccgag atcggcactc acccgctcga tggtctcgcg ggccgtgtcg cacaggtggc
     9301 accccggccg gccgatcagg gtgacccgca cgggaagcgg ctgcgcggcg tccatcagaa
     9361 cgcgctgtcg gtgagaacgc gggtgtccat cagaagaaga ccgaacgccg ctgcatgagc
     9421 agggtgtaca gggtctgctg gatcgtctcg cgcacctggt cggtgaggtc gaagacgagc
     9481 atcgggtcgt cggcggcgcc ggggccctgg ctgtcggtgg ggatgggggc accgaactcg
     9541 atgatccact tgctcggcag cgggatcaga ccgagcgggc cgaacagcgg gaacgtcggc
     9601 gtgatgggcg cgtagggcag cccgaacagg cgggccaccg tgcccatgtt gccgatgatc
     9661 ggggcgatct cctcggcacc cacgaccgag cacgggatga tcggtacctg cgcggccagc
     9721 gcggcagcca cgaaacctcc gcgaccgaac cgctggagct tgtagcgctc actgaacggc
     9781 ttgccgaccc ccttgaagcc ctcggggaag accccgacga gctcgccctg ggcgaagagg
     9841 cgatcggcgt cggagttggt ggccagcgtg gagcccgacc ggcgcgcgac ggtgccgagg
     9901 aacggtgtct ggaagacgag gtcggcgccc agcgcccgca tcgcccgccg ctgggggtgc
     9961 tcgtcgtgga tggcgagctg caccatgagc gagtcgatgg cgatggtccc ggagtggttg
    10021 gacacgacga gcgcaccacc ggtggccggg atgttctcga tgccgcggac ctcgacgcgg
    10081 aaccagtgcc ggtagatcgg tcgcagcatc gggtagaaga cgttctcggt gaagtcggcg
    10141 tcgtagccga agtcatcgac gtcgtagtcg ccgtcgatcc ggcggcggat gtaggccagc
    10201 acctgcgcca cgcgacgctc gatgtcctcg ggcgagatgc ccgcggcctg cgccccgaac
    10261 cgcagcaggc cgactgccgc ggacagggcg tccccgacgt cgggcagcgg cagcccggtg
    10321 cgctccggcg cctcgatcac cgtcgggtcc agcggcggct cggcgatccg caggtgcggc
    10381 ctcgcggcgt cgcggctggc cttctcgcga gggttcagcc gaacccgggg agcccggggt
    10441 gcctctgcga ccggggcggg cgcggacctg gcgtgccgct cgccggcctg gcgcgaggcc
    10501 cggcgggagg gagccgagcg caccttgggt gccgggcggt gcggccgacg agctgcctgc
    10561 tcccccgtgc ccgaatcgac cgattggtca ctcggggtac gcgccacctt ctgcccagcc
    10621 gtccccgaat cgaccaattg gtcactcggg gtacgcgcca ccttcttcgc ggctggcttc
    10681 ttcggcgccg ctggcttctt cggggtcgtc ttcttcgggg tcgcctgctt ggcggctggc
    10741 ttcttcgtcg ccgacgcgac ggcatccgcg gtgtcgccgg caggcacgac gggctcggca
    10801 ccgatcagcg gggtggagcg gcgcctggcc cgctcccccg aggcccgggc cgcgcctgcg
    10861 gcgagggctc cctgtcgcgc tgccatcaac gtccccttcc tgcgccgaga aggccgagaa
    10921 cgacctgact cgccgtgcct gcgaccccgc tcacggcatc cccgacgatg gcggcggcgg
    10981 gcaccgcggg gccgacggtg cgggcaaagt cctcgaacgc ctcacgggag gtgtagcgcg
    11041 gggtgaagcc gaggacggag cgcatccgcc gcgtgtccat gccgcgaccg aacgcgagga
    11101 aggacatctg gtccggcgag aagtcggcca ggccgagccg tttggcgaac ccgccgacga
    11161 cgccggcagc ctgtagcggc atcggcacca ccggtcgtcc gacgatcgct gcggcctgga
    11221 gcaccgtcag cacgccgtca cccgcgatgt tgacgatgcc ggaggcctcg ccggtggtgg
    11281 cgcgcacgag cgcgtcgatg gcgtcgtcct cgtggacgaa ctgcatgcgg gcgtcgtacc
    11341 cgagcggcac cgggacgacc ggcaggttga agtagtcggt gatcgacgtg cgaatgcgcg
    11401 gaccgacgat gttggcgaag cgcaacgtga ggatctcggt gtccggacgg cggcgggcga
    11461 acccgcgcac gtacccctcg acctcgatgg agtccttgcc gaacccgccc gagggcgacc
    11521 gcttggcgcc catgtcctcg gtgaacatcg ccgggtcacg gggcgtggag ccgtagacag
    11581 ccgcagacga cttcacgacg agacgccgca ccgtcgtcga cttctggcac gccgcgagca
    11641 gctgcatcgt gccgatgacg ttgatctcct tctgcggggt gcggcccccg acgaaggtgg
    11701 gcgtcgcgat gacgttcatg tgcacgacgg tgtcgacccc ggccgactcg atgacccggg
    11761 cgatcatcgg gttgcggatg tcggcgcgca cgaactcgac gccgctgatg tcgtgcgcgg
    11821 gcgggatgac gtcgacgccg accacccggt ccacgctcgg gtcagcgctg aggcgacgcg
    11881 cgaggagtcc tccgaggtag cgggagaccc cggtcacgag caccactgac gccatgccct
    11941 gctccttcac ctgatttcga cagccccgac cctaatcgac ccgtcggcgc accgacaggg
    12001 caggtccacc cggacacgac aaaggccccc ggggtgcggg gacctttgaa gtgcagaggt
    12061 gactcttacg tcacttcttg ttgcggcgct ggtggcgcgt cttgcgaagc agcttgcggt
    12121 gcttcttctt cgccatccgc ttgcgccgct tcttgatgac tgaacccatt gcgatgtcct
    12181 cgtcgttaag cgtctgatgc ggctgcccac cctaccggca ggcggtcacg ctgcccaaac
    12241 cgtggccacg gcgctcgttc ggcagcgccc gtcgggcaca cccgtgacca gtcccgaaca
    12301 gccgtgaggg gcggcccgca cgtcgggccg ccccccaccg gtgtggttcg tcggggtcgg
    12361 tcacgccgtg tcgacgaacg aggtgcgcag gtacgcatgc acctcgtcct cggggacacg
    12421 gaaggaccgc ccgacgcgga tggccggcaa ctccccggcg tggaccatgc ggtacaccgt
    12481 catcttggac acccgcatga ttgcggcgac ctcggcgacg gtgaggaacc gcacctccgc
    12541 gagctgacgc tccgtcgaca tcgtgacctc attctcctgc acgaccacag cgccaggacg
    12601 ccccggcagt ctccctgtcg tcgtgcaagc gcaaccatag gggcaggtgt gaccactggg
    12661 gaagtggaac gacgaagttg ggcgccaatg tccagtcgac acgccgtggc ggatgccgtg
    12721 agtccgctcc cagcggcatc cgccactcgc ttcacatcgg ggctggcgcc gcagccctcc
    12781 cgtcgagagg aggcaacacg cagtggcgag gcgcacggca tccgcgtgtt gcctcctgtc
    12841 gacccagcgg tcagtcgaac cagggatcca gcccgtggtg gctgaacacc gcctgacggg
    12901 ttgccatgat ggcccggtcg atcgggctct cggggttgtg gcccatgctc cacggctgca
    12961 cccagatggg caggctctcc tcgggcaggt cacccatccg ggtgggagcc gagcgaccca
    13021 tggtcgcctc gacgtggtcg agccactcct gcggcacgcg agcctgggcg gggacgggga
    13081 cgtgggcggc gatggcggtc aggtgggtcc atgaccgcgg gacgacgtcg acgagctcgt
    13141 agccaccgcc gccgagagcc acccagcgcc cgtcggccgc ctcgtgcgcg aggtcgtgca
    13201 acgtctcgtg ggcccgacgc tgggcgtcca tggtgatcgt caggtgcgcc agcgggtcgt
    13261 gcatgtgggt gtcacacccg tgctgggtga cgagcacgtc ggggtggaag gcgcgcacga
    13321 gcggcgggac gacggactcg atggaccgca accacgcggc atcccccgtg cccggtggca
    13381 acgcgacgtt cacggcactc ccgcgggccg acgggccgcc gatgtccgag ggccacccgg
    13441 ttcccgggaa gaggtgctgg ccgttctcgt gcaccgagat ggtcagcacc cgcgggtcgt
    13501 tccagaagat cttctcgacg ccgtcgccgt ggtggacgtc gatgtcgacg taggcgacgc
    13561 gctgcgcccc gttgtcgagc agccactgga tgccgacggc gatgtcgttg tagatgcaga
    13621 agccggacgc gtgggtgcgc atcgcgtggt gcatgccgcc gcagaagttg accccgtggt
    13681 cggtctcgcc ggtcagcacc tgacggcaga ggtcgagggt gccctgggcg atgcgggccg
    13741 atgcctcgtg catcccgggg aaggcggggt cgtcctcggt gccgagccct cggtggacgt
    13801 cagcagccat cgggtcggcc gaggctgccc ggaccgcggc gatgtagtgg gggtcgtgca
    13861 cggtggccag cacctcgtcg gtggcggcct gcgggctcag caggtcgacg tggtccagca
    13921 cccccagggc gcgcgcgagc cgggtcgtga ggtcgagacg caccggggcc atggggtgcg
    13981 aggggccgaa gttgtacgcc gtgaactcgt catcccagac gacgctcgtg cgatgggcca
    14041 tggtggcaac ctactcccgc agaagtcccg tggcaggctg ggacccctca cgttccactt
    14101 ttgcaggagg gcaccaccga tggctcgagg cagcggcacc gagaacgacg tcctcgtcat
    14161 cggcctgggc cggttcggcg cagcggcggc cctcgagctg cgccgcctgg gccacaaggt
    14221 caccgccatc gagaaggacg gcttcctcgc cgagaagttc ctcggccgga tcagccacgt
    14281 gatccacggc gacgcgagcg acgtcgccgt catccgcgac gccaaggccg gtcggttccc
    14341 catcgccgtc gtggcgatcg gctcctcggt cgaggcctcg gtgctggcag ccgccaacct
    14401 cgtcgacgcc ggggtggccg acatctgggg caaggccctc tcacccgagc acgcccggat
    14461 cctcgaccgg atcggtgtgc accacatcgt gcgacccgag gccgacaccg gccgccgggt
    14521 ggcccacctc atgggcgggc ggatgcagga ctacatcgag ttcgacgacg ggttcgccat
    14581 cgtcaagatg cgccccccga gcgagacggt cggcttcacg ctcgagcaga gcgccatccg
    14641 cagcaagtac ggggtgaccg tcgtcggcgt caaggcgccc ggccaggact tcacctacgc
    14701 cgtgccgtcg acgaaggtct cggcccacga cgtgctgatc gtcagcgggc cgacgacact
    14761 catcgaacgc ctggccgccc gcccctgacc gctcagaact gagcgatgcc gccgcggtcg
    14821 ccggagagcg gcagggtcat cgccagctct tcttcaccgt catcggacga gacgacccgc
    14881 atcggccggc gctcggcggt cgggcggaac ccgaaaccgc tggcgaaggc cacggcccgc
    14941 ccgttgtcgg tgccgaccca gtagtgcagc tggcgcttgc ccttcgcggc ggcagtctgc
    15001 gcaccggcgc gcacgagggc cgtggccacg cccgtacccc gcgcctcggg cgccacccag
    15061 agaccgaaca gctgagccgt ctcgggctgg tcgtcgtcgg cggatccgac gctcgcgatg
    15121 cccacgggct cgccgtccac ctccgccagc aggcgggtgg agcgctccat gcgttcgcgc
    15181 cagaactccg ccggttcggc ggactcagcc tcgtgagaag cgacgaaggc gtcgggcgac
    15241 tcctgcagcg cggtgaggcg cacggagcgg tatcgctccc agtcgtcggc ccccagcggg
    15301 cgcacggtga tctcactcat gcccgccact ctcccacggc cccccacgcg ggcggcaact
    15361 cgggcaggtg aactctcagg atcgcgcggt caggtcgagg accatctgct cctcggtcac
    15421 ggcggggtcc cagggcatcg agttgacctc cccggtgggc cgaaaaccga tgcgggagta
    15481 gaaggcgcac gccctgacgt tctcgtgggc gacgtcgagg acgacgcggc gcaccccggc
    15541 atccgaggcc gcgcgcaggg ctgttcgcac gagccgttcc gcgacgtccg tccctcggac
    15601 aacgctggcc acccacatgc cgacgagggt gctctcgtcc tccggctggc cctcaccgcg
    15661 ccacagcccg acggtgccca cggggcgggc gccatcgaag gccagccagg tccgggggcc
    15721 ggtctcgagg aaggccgccc agtcgtcgtc ggtgcgggcc ctcgcttgct cgtaggtgct
    15781 ccagaacgcc cgcgggctgt cgatgagcgc cgcgatgcgc acgtcgcggt aggtcggcag
    15841 cagcgcagcc gtcacctggt cgatgcggat gccgtgcgca cccgtcggcg agccgacctc
    15901 accgcccgcg gtgggttggg tcggcggggt gggtgcgtcc acggcgtcac tccccagcga
    15961 gctcggccga ccgccgggca gcggcctcca tggccgtgat gaaggccgcg cgcaccttgt
    16021 ggtcatcgag cgtgcgcagg gcggccgcgg tggtgccacc gggcgaggtg acctgctcgc
    16081 gcagcacggt ggggtgctgg ccggtctcct tgatcatcgt cgccgcaccg aacagggtct
    16141 gcacgaccag ttcggtggcg gtggtgcgcg gcatccccag caccaccccg gcctcgatca
    16201 tcgcctcgac gacgtagaag atgtaggcgg ggccgctgcc ggagatggcc gtgacggcat
    16261 cctggagctt ctccgggacc tcgacgaccc ggcccgtcgc ggcgagcagc gcctgggcct
    16321 cggccaggtg cgcggtgtcg cagtgggcgc ctgcgctgac cgcagccatc ccctggtcga
    16381 cgagcgcggg ggtgttcggc atgacccgca ccacagggcg accctgcggc aggcgcgact
    16441 cgaggtagga cgtcgtgatg ccggcggcca ggctgacgac gaggttgccc tcggcgacgt
    16501 ggtcgtggat ctccgcgagc agggcgccca tgtcctgggg cttgacgacg agcacgaggg
    16561 tgtcggcgag gtcggccgcc tcggcgttgt cgagcacgcg cacgccgtac ttctcggtga
    16621 gctcggcggc ccggtcggcc cgccgctcgg tgatgaccag ctcgtcgacc gggcgcccgg
    16681 agcggatcaa ccccgacagc agggtctcgc ccatgacacc ggcaccgaag atcgcaacca
    16741 ttcccatggc aggtgagtat gcccggtggc gaggacagcg ctccgcgagt ttcggcaggc
    16801 gggtgcgcca cggcatccgg tcacccgatc atccgctgta cggatcagcc gttggtggcg
    16861 agtgagcgca ggaagaactg cacgttggcc gggcgctcgg ccatgcggcg cacgaggtag
    16921 ccgtaccagt cctcgccgaa cggcacgtag acccgcatct ggtggccgcg gtcggcgatc
    16981 cgcttctgct cgttcatccg gatgccgagc agcatctgga actcgtagct gtcgggggcg
    17041 cgcccgtggt ggccggcgag cgcggcggcg atgtcgatga ggcgcgggtc gtggctcgcc
    17101 accatggggt agccgtcgcc ggccatgagc accttgaggc agcgcacgta ggacaggtcg
    17161 acgtcgtcac cgtcctggta ggccacggac gccggttcct tgtacgcgcc cttgcacaac
    17221 cgcacccgac tgcccgccgt cgcgaggtcg cggcagtcgg cctcggtgcg gtgcaggtag
    17281 gactggagca cggcaccgac ccacgggaag tcctcgcgca gcgcgtgcag cgtggcgagc
    17341 gtgcggtcgg tcgtcgtgtg gtcctccatg tcgagggtga ccgtcgtgcc agcggcctgc
    17401 gcggcctcgc agatcgccct ggcgttctcg agggcgatgg catcgccgtc agccggcagc
    17461 gcctgaccga tggcgctgag cttcacgctg acctcggcgg caccgtatgc cgtgaggtcg
    17521 gcctcggcga gcaggtcgag caggtgcacg taggtgtcgc gggtggtggc cgcctgcacg
    17581 gcgtcggtgg tgtcctcgcc gaggtagtcg atggtcgcca gccggttcgt ggtgcgcagc
    17641 tcaccggcga cggtcacggc atccggcacg ctctcccccg cgacatagcg ggcgacgacg
    17701 gcgcgactga cgggggccgt caccaccgcc ttgcgcacga ggtcggactc ggagatctgg
    17761 agcatcgctc cgcggagcag gtcgctgggg tccacgacgt caggctagcg ccgccgccca
    17821 cgccccgctg ggtcacgggg tgcggcggcg cagcgtcagg ctccccagca cgagggccag
    17881 ggcgatccag ccggtcacga cggccaggct cggcagcacg tcagcgagcg gatcggccag
    17941 caccgtgacg tccttcatcg cgtcgacggc gtaggacagc ggcaacacgt ccgagatcca
    18001 gcgaagcact gacggcagct ggtctcgggg cagcaacagc ccgcacagca ggaactgggg
    18061 cagcacgaag gccggcatga actgcacggc ctggaactcg gtgcgcgcga agccgctcgc
    18121 gagcaacccc agtgccgtcc cgagcagggc gtcgagcagg gcgatgacca cgagcagcca
    18181 gatcggcccc gcgatggcca gcccgcagac ccagaccgcg aacccggtgg ccaccagcgc
    18241 ctgcaccacg gcgacggccc cgaaggccag cgcgtagccg agcatgaggt cacccttggc
    18301 caggggcgtg gtgagcagcc gctccagggt gccggaggtg cgttcgcgca gggtggcgat
    18361 gctcgtgacg acgaacatga cgacgaacgg gaacaccccg agcagcgccg gtccgatccg
    18421 gttgaagacc tcccccggcg cgtctgcgta catccacgcg agcagcccga gcagcacgca
    18481 cgggacgacg agcatgaggg ccagggtgcg gtggtccctg cgcagctggc gcaggatccg
    18541 ggatgccgtg gcgagcgtga tgcgggcgtt cacgccgcgt cctcgggctg ggcggtctcg
    18601 gccgcggcgt tcgcgtgggc ggcatccgcc gcgtcgatga gggcgaggaa ggccgcctcg
    18661 gcgtccgcgg ccccggtggc ctcgagcagc tcgggcaacg tgctgtcgct gagcacccgg
    18721 ccctcgcgca gcagcaccag gcggtcgcac cgcaccgctt cgtccatgac gtggctggac
    18781 acgaacaggg tgtggccttg gtcggccaac cgatggaaga tcgcccacag ctcgcgccgg
    18841 agcaccgggt cgagcccgac cgtcggctcg tcgagcacga ggacctcggg gtcgccgacg
    18901 agcgtgcacg cgagactcac ccgcgcctgc tggcccccgc tgaggttgtc gacgcgggcg
    18961 gcggcgtgac tggtgaggtc gaccgccacg atggcgcggt cgacggcatc cgcgggtgcg
    19021 cccaggacgg cacggaagta gtcgacgttc tcgcgcaccg acaggtcgcc gtagacgctc
    19081 ggggactgcg tcacgtaacc gacgcggtgg cgcagggacg gtgacccggc atcctcaccg
    19141 agcaccagga cgcggccact ggccaccacc tgcaccccga cgatggcgcg catcagcgtg
    19201 gacttgccgc caccgctcgg cccgagcagc ccgaccacct gcccgcgagg cacccgcagg
    19261 tcgaggtcgg gaagaacctc gcgcgagccc cgcaccacgc gcaggtgctc gacgacgatc
    19321 gcatcaccct ccaccttatt cgtcacatga tgaattctgc acctcccaac ccgtccggac
    19381 aaggagggtg tgatccacct cccccatcgt caggtggcga ggtggaggcg cgcgaacgcc
    19441 agcgactcgg cgaggtccag ctcgcgctgg gccgcggacg cccgcctggt ccccacctcg
    19501 acgacgacgt ggccgccgaa ccccgactcc gccaacagct ccagcacctc cccgcacggc
    19561 tgcgtccccc gcccgggcac gaggtgctcg tcgcgcggcg accccacccc gtcggcgagg
    19621 tggaggtgcg ccaaccgggg gccgagatcg tgaaccatcc ccacggcatc cgaccccgcc
    19681 gtcgccgtgt gcgagaggtc gagggtcacg tggtcgtacg gctgcggcac cggatcccag
    19741 tgcggcaggt acgcctcgac ctcgcgcgag cggggccccc gccacgggta catgttctcg
    19801 accgcgagcc gcatgccggt gtcgtgctct cgcagcgcca ccccgtcgat gaaccccgcc
    19861 gcgtactccc gctgccaccg gaacggcggg tgcagcacca ccgtgtcggc gccgacctcg
    19921 acggccagat cgatggaccg gtcgatcttc acccaggggt cattgcccca gacccgttgc
    19981 gtcacgagca gcgtcggggc gtggatcgac aggatccgga tgccgtgcag ctcggcgagc
    20041 gctcgcagcg caccggcctc gcgggtcagc ggatcggtcc agaccatgat ctcgacgccg
    20101 tcgtagccga gccgggcggc cagctcgaag gcggcggcgc acccctcggg gtagaccgac
    20161 gacgtggaca gcccgatcag gggcgccggc actcccacgg gatgggtccc ggtgcgcgcg
    20221 ccacggccgc gggtcgccac ggctcagctc agcctgtcca ggtggcgcag gatgatgccc
    20281 tcacgcagcg cccacgggca gatctcgagc tcgtcgaggc cgagcaggtc catggcggcc
    20341 tcggcgacga tggcaccggc gagcagttgg tgtgcgcggg atgccgagac gcccggtagg
    20401 tcggcgcgct cggcatcggt catggcggcg atctcgggca gcttctcggt gagcagcgcg
    20461 cgcggcagca cccgggcggc atacatcccc tcgctgctgg gagcggcacc gcagatccgc
    20521 gccagcgagc gcatcgtctt cgacgtgccg accgcgtggt cgggggcacc ggccctggcg
    20581 agcggacgca gctcggccgc gatcgaggcg cgcaccagcc gccgcgcctc acgcacggca
    20641 tccgcactgg gcgggtcgcc gggcagcagg tcccgcgtca cccggccagc gccgagaggc
    20701 agcgacaccg cgacgtcagg gtcctcgtcc atgcccatcg cgagctcgag cgagccgccg
    20761 ccgatgtcgg ccacgagcag ggtgccggcc gaccagccga accagcggcg tgcggcgagg
    20821 aacgtcagcc gggcctcgtc ggccccgctg aggacgtcga ggacgacgcc cgtgcgctcc
    20881 ttgacctcga gcagcacctg gtggccgttg ggcgcttccc ggatcgcgct ggtgacgaag
    20941 ccgctgaggt tctcgacgcc ctggtgctcg gcgacctcga ggcactcctg cacgaacgtg
    21001 gccaaccggt cgctgccctc gtcgccgatg tgcccgtcgt cggtgacgtg ctcggacagg
    21061 cgcaggtcga tcttgtgctt cgtcgccggc aggggctggg ctccggggtg cgcgtcgacc
    21121 acgagcaggt ggaccgtgtt cgatccgacg tcgatgaccc cgaggcgcat gcgccaaacg
    21181 tagtcgctgt tcggtagccg gcccataggg acggcacggt ggggccggcc acagccagca
    21241 gggtgggcgg ctcatgggga cggggcggcg ggtggcccat agggtggtcg cgtgcccgag
    21301 acgccgctgg acatcgcccg cacctgggcc gagctacccg atcccgcgaa tcccgagcag
    21361 cgcttccggg tcgatctcac ctggctgacg tcgagctgga cgtgcatctt cggctcgggc
    21421 tgcaagggca tctacgccga tcgccccgac gacgggtgct gcacgctggg cgcgcacttc
    21481 accgacgacg aggacttcga gcgggtgcgg gccgtcgtcg ccgagctcgg cgaggacgag
    21541 tggcagtacc accccggccg cggcaagcgg ggcgacaagg acccctcgcg ccgctcggcc
    21601 tggaccgagc tcgaggcctc gccgggcggg tcggacggat cggacggatc ggacggatcg
    21661 ggcggatcgg agggatcgga tgccgcccgc aagaccaagg tggtcgacgg cgcctgcatc
    21721 atgctcaacc gccccggctt tcccgctggt gccgggtgcg cgctgcacca gcacgcggtg
    21781 ctcaccggcc ggccgccgca cgaggccaag cccgacgtct gctggcagct gccgatccgg
    21841 cggtcgtacc gcaccgtcga gcggcccgat gacacctcgt acctcgaggt gaccatcgcc
    21901 gagtacgacc ggcgcgggtg ggggcctggg ggccatgacc tcgactggta ctgctcgtcg
    21961 aacaccgagg cccacgtcgg ggccgagccg gtctaccgca gcaaccgggc cgagctcact
    22021 gccctcatgg gtgaggccgc ctacgccgcc ctggtgctgc gctgcgaggc ccacctcgac
    22081 acggtcaagc gggcccgttc agcgggggct cgacagctga tcccgctgct cgtgcacccg
    22141 gccacggtgg aggccggcac ggtgggcggg cggcgcacca gggtgcctcg gcgccggccg
    22201 gcgacagctg ccacccggac ggccaaggtc ggcaccccca ccaaggctgg ctcgaccacc
    22261 aagtcgacct cgaccaccaa ggcgacctcg aggacgacgg cggcctcccc cacgaaggcc
    22321 ggcgcgacca ccaaggcaac caagtccacc gcgaagccca ctgcgaagac ctcgccgaag
    22381 gccccaggca agacgacacc gaaggccgcc ggcaaggcga cgcagaaacc tgccagcacg
    22441 tccacaccga aggcgtcggg caagtccgtc gccaaggcct ccggcaagac ctcccccacg
    22501 tcgtgatccg cagccccaac atctgggact cccccgacgt ctacgaactc gagaacctcg
    22561 cgtccgaccg cgccggggtc atcgatctgg ctctcaacgc gatccatccc ctggatggtt
    22621 cagtgctgct cgacgtcggc tgcggcgccg gcttccacct cccccggttc gccgcgcgcg
    22681 gagcccgggt catcgggctc gagccgcacc tgccgctcgt ggagctagcg cgcgcccgac
    22741 tcgccgctgg tgcggacggc gggccagcgg catccgtcat cgccggtgac gccgagcgga
    22801 tgccgttgcg agacagcagc gttgacgtcg cccacgcgcg ctgggcctac ttcttcggcg
    22861 cgggctgcga gccgggcctc agcgaactcg ccagggtact gcgcccgggc gggacggcgt
    22921 tcatcgtgga caacgacgtc acccggtcga cgttcggcgc gtggttcagc cgtgcgtacc
    22981 cggcctacga cccggccggg gtgcagcggt tctgggaccg gcaggggttc accaccgacc
    23041 acctcacgat cgagtggacc ttcgacaccc gcgaggacct cgacgccgtc gcgcgcattg
    23101 aactgccgcc ggcggtggcg gatgcggtgc tggccaccca cgacgggctg accgtggact
    23161 acgccgtggc cctgcgctgg aagcgcttct gagccgacct gcacctcccc gcgccgccgg
    23221 acgtgcaaaa gggtgcccgc tggatggaaa ccccagcacg agtgcgggtg cgacgtgtgt
    23281 ggcatcgcct ttgcacgtcc agcggcgcac ccgacgccag tccagcgagc gcaggcaggc
    23341 tccggatgcc ggtcgcggcg ccttgcggtg gcagactcga ccttgtcggg cgcgagccgc
    23401 ccacccagac ctgttccacc ccgcgcactg tgctgcgcag ccatcgatcc cccagccgga
    23461 aggcccccag tgacgcgtca ccaccccatg atctccatct cccccgcagc gcaggccacc
    23521 cgatgagccg ccgctcaccg cgccgcaaga gcagcgggtc gctgccccag ctgccgccga
    23581 acggcctgcg ggtcatcccc ttgggcggcc tgggtgagat cggccgcaac atgaccgtct
    23641 tcgagcacca gggtcgcctg ctcatcgtcg actgcggcgt tctcttcccc gacgagagcc
    23701 agcccggggt cgacgtcatc ctccccgact tcacctggat cagggaccgg ctcgacaagg
    23761 tcgacgccat cgtcctcacg cacggtcacg aggaccacat cggcggcgtg ccctacctgc
    23821 tgcgcgagcg cccggacatc cccgtcgtcg gctcccgcct gaccctggcg ctgctcgagg
    23881 ccaagctcac cgagcacaag atcacccccc gctcggtcgt cgtcgccgag ggtgatcgcc
    23941 ggccgatggg gccgttcgac ctcgagttca tcgcggtcaa ccactccatc cccgacggcc
    24001 tcgcggtggc catccgcacc gctgccggcc tggtgctgca caccggcgac ttcaagatgg
    24061 accagttccc gctcgacggc cgggtcaccg acctgcgggc cttcgcccgg cttggcgagg
    24121 agggtgtcga cctgttcctc gtcgactcca ccaacgccga ggtccccggc ttcatgatct
    24181 ccgagggtga gctggccccg gccatcgaca cggcgttccg caacaccaag ggtcgtgtca
    24241 tcgtctcgag cttcgcgagc cacgtgcacc gcatccagca ggtgctcgac accgcccaca
    24301 gccacggccg caaggtcgcg ttcgtcggcc gctcgatggt gcgcaacatg ggcatcgccc
    24361 gcgagctggg ctacctgaac atccccgacg ggctcgtcgt caaggacctc aaggccctgg
    24421 accggctgcg ccccgaccag cagtgcatcg tcgccaccgg ctcgcagggt gagccgatgg
    24481 ccgccctggc acgcatggcc aaccgcgacc acccggtgag catcgccgcg aacgacaccg
    24541 tgctcatggc ctcctcgctc atcccgggca acgagaacgc catctaccgg gtgatcaacg
    24601 agctgacccg ctggggcgcc aacgtcgtgc acaagggcaa tgccaaggtg cacgtgtccg
    24661 ggcacgccag cgccggcgag ctcgcgtact gctacaacat cctcaagccc accaacgtgc
    24721 tgcccgtgca cggcgagtgg cgtcaccttc aggccaacgc cgagatcgcc atccgcaccg
    24781 gcgccgagaa ggaccgcgtc ctcatcgccc aggacggcag cgtcatcgac ctcgtcgacg
    24841 gccgcgcgaa gatcaccggc acggtgcctg cgggcctggt ctacgtcgac tcgatgacgg
    24901 tcggcggagc caccgaggag acgctgcacg tgcggcgcac cctcgccgag gagggtgtcg
    24961 tcacggtggt ctgcatcgcc gaccccgaca ccggcaagct caccgaggac ccggactacc
    25021 tggcccacgg cttcccgccg gacctgctcg acttctccgg cgcgaccccg gccatccaga
    25081 acgccctgac caaggcggcg aacgacaaca tcgaggacct cgagcagatc gaggacctcg
    25141 tgcgccgggc cgtgtcgaac tgggcgcacc gcacccaccg gcgcagcccg ctcgtcatcc
    25201 cggtcatcgt cgacgcctga ccgtcgcccc ccaacctccg acttcgtgcg atttcggtgc
    25261 gcagattggc atcaacccgg cccttcaccg cacccaagtc gcacgaagtc gggtttcagg
    25321 cggggcaggg gctagctcgg gtcggggtcg aggtcgaggg cgatgtccag gatcggcgag
    25381 gagtgcgtca gcgcccccac cgacaggtag tccacgccgg tcgccgcgta cgccgcagcc
    25441 acctccaggg tcagcccgcc cgtggcctcg acctcgacgt actcgcccgt ggcgcgcacg
    25501 gcatccacgg tcgtgcgcag caggtcggtc gacatgttgt cgcacagcag gaaccgcgcc
    25561 ccggcagcaa cggcctccag cgcctcagcc gtcgtcgtca cctcgacctg gatgtcgacg
    25621 tggggaaacg cagcccggat gctgtcgtac gcggccgtca gcccaccggc ggcgagcttg
    25681 tggttgtcct tgatcatcgc gacgtcgtag agccccatgc gcttgttcgt gccgccgcca
    25741 cagcgcacgg cgtacttctc cagcgcccgg atgccggggg tcgtcttgcg cgtgtcgagc
    25801 accatcgcgc ccgacccggc caaggcatcc gcccaccgac gggtgtgcgt ggccaccccg
    25861 gacgtgcgcg agagcacgtt gaggatcgtg cgttcggaga tcagcgtcgt gcgggtcgac
    25921 ccacgaaggg tggcgaggtg gtcaccgcgc accacggcat ccccgtcggt gacgtgcagg
    25981 tcgacccaga cgaccccggt acccaggcgg gcgtcgaccg cctcgacgac gagcgcgacg
    26041 acgggcagcc cagcgacgac gccgtcagca cgggcgacga ggtgcgagac cgactcctgc
    26101 tcctccggga tggttgcgac ggtggtgacg tcggtgccgt cgccgaggtc ttcgtccagc
    26161 gcccgctcga cgagcaggcg ggcgtcgtcg agggggaaca cgtgctcgct catcgggggt
    26221 ctcctgcggg gcgttcggtg atgacgacct gaccgtcggc ccggcgcgtc gcggtgaggt
    26281 gggtcagcca gcgggcgtcg tcgcgctcgg ggaagtcaga gcgcacgtgc ccgccgcggg
    26341 tctcctcgcg caggtgcgcg acgcgcgaga gcacctgtcc gaggtgcagc aggttggtcg
    26401 tctcccaggt gagcggcccc ggttcggcct ccgtggtcgg ctgtgccgcg agtgccgcga
    26461 gttcggcttg ggcacccgcg gtcgactgac ccgagcgcac cggccccgag cccagcgtca
    26521 tggcgcgctg cacctcgatg cgccgcgagg cgtcgagcac ggcatcctca ccgggtgtcg
    26581 agccgacctc acgctgcggc aggtcgccgg agctgaaccg ggccgcgatg tcgtcggcga
    26641 tgcggtgggc gaagaccagg ccctcgagca gggagttgga ggcgagccgg ttggcgccgt
    26701 gcacgccggt gcacgagcac tcgccgcagg cgtagagccc gtcgagcgac gtgcgcccga
    26761 gcaggtcggt ctcgacgcca cccgaggcat agtgctgcgc cggcgccacg ggcagcaggt
    26821 cgacggccgg gtcgaacccg agctcacggc agcgggcgac gatcgacggg aaccgctgct
    26881 ccaggaacga aacgccgagg tgtcgcgcgt ccagccagac gtggtcgacc ccttggtcgc
    26941 gcatgacgtc gaggatggcg cgcgcgacca cgtcacgcgg cgcgaggtcc tcgaggggat
    27001 gccgtccggc catgatccgg gccccgtcac cgtcgacgag gaacgcgcct tccccgcgca
    27061 cggcctcgga gatgagcggc tgctgccccg tcgacccctc gccgaggaac agcaccgtgg
    27121 ggtggaactg gacgaactcg aggtcggtca cccgggcccc ggcgcgcagg gcggcggcca
    27181 tcccgtcgcc ggtcgcgacc ggggggttgg tcgtcgaggc gtagatctgc ccgaggcctc
    27241 cggtggcgag cacgacggcc cggcccgcgg ccgcgccgac cccgtcgacc tggccctcgc
    27301 cgatgacgtg cagcgtcacc ccacacacgg cgccgtcggc ggcggtgagc aggtccacga
    27361 cgagggcgtg ctcgatgacc tcgatgcccg ggtcgtcgag cacccggtgc agggcggcga
    27421 tgagcgcccg ggagatctcg gcaccggtgg cgtcgccccc ggcgtgggcg atgcggtcgc
    27481 ggtggtggcc accctcgcgg gtcagcttga tctcaccgcc ggcgtcacgg tcgaactcgg
    27541 cccccagggc gacgagttcg tgcacccgtt cggggccctc ggtgaccagg gcccgaaccg
    27601 cctcgacgtc gcacaggccg accccggcga ccaacgtgtc gtcgaggtgc tcctgcgcgg
    27661 tgtcgtcggg gtcgagcacg gcggcgatcc ccccttgggc ccactgggtg gagcccgcgg
    27721 agagcaccgt cttggtcacg agcagcaccc ggtcgacctg ctcgcgcagc cgcagggcgc
    27781 acgtcaggcc ggcgatgccg gagccgacca cgatgacgtc ggcggacgtg gtccaccccg
    27841 gtgcgggggc ggtgaggcga cggggcagcg tgaggggggc ggacggcatc cggaatgcct
    27901 ttcagacgtc cgcgcgggcg tgcgggacgt cggagaagac gtcgagtgcg cccccgcccg
    27961 gcccgacgag cacgggggtg ttgtcgatga ggcgggtggt tccgacgcgg gcggcgacgg
    28021 cgagcagggc ctcgccccgg taccactcgg gcacgtcggc gagcgtgtgc gggtgcacga
    28081 gcacgaggta ggagacgagg gccagcggct cctcgacgag cacctcgcgg gcggcgcgac
    28141 ggatggcgga ggggccgaag gcggccgcgt cggcacccgc gcgcagggcg ccgctcaggc
    28201 agagcgcgac ctcccggtcg gactcggtga ggtacatgtt gcggctgctc atcgcgaggc
    28261 cgtcggcttc gcggacggtc ggcaccgaga cgacgtcgac ggggaagtcg aggtcacgca
    28321 ccatgcgacg gatgaggagc agctgctggg cgtccttctg gccgaagtag gcgctgcggg
    28381 ccgcggtgat gtgcaggagc ttgccgacga cggtgagcac gccgtcgaag tgaccggggc
    28441 gcgatgcccc ttcgagcacc tcgccgagcg ggccggcggc gacgcgcacg ccggggtcgc
    28501 catcggggta gacgacgtcg ggggtcgggg cgaacacgac gtcgaccccg gcggcgcggc
    28561 agatctccat gtcggcgtcg aaggtgcgcg ggtagcgcga gaggtcttcc ttcgggccga
    28621 actgaagggg gttgaggaag atcgtcacga cgacgtggtc atggcgttgg cgggcggcct
    28681 ggatgaggcg ggcgtgaccg tcgtgcaggg cgcccatcgt catgacgacg gcgacgtcac
    28741 ggctcgacaa cgcctcgcgt gcggccttga actcctcgcg ggtgtgggcg acgacggggg
    28801 ctgcgctctg ctggtcactc acggctgggt cctgtcggcg agggtttcga ggagtggttc
    28861 ggcggatgcc gggcgcaacc gtccgctgtc gagcgcacgc agcgctgttg ctcgcgccat
    28921 cgccacgtac gtcctccgga tctcgggcga gagccggtcg agctcacgca ggtggtcctc
    28981 caccgtaccg acgtcccccc gggcgacggg cccggtcagg gcggcatccc ccaggcgcag
    29041 ggcgttgtcg agagcggcgc gcatgagtgg cccgaggaca cgttgcgggt cctcgacccc
    29101 ggcgctggcg aggatctgca tcgcctcgct cgtcagggtg accaggtggt tcgcgccgtg
    29161 tgccagggcg aggtggtagg cggtgcggtc ggtctcggcg atccagacgg gttcgccgcc
    29221 catctcgagc acgagggcct cggccatcgg gcgcagcgcg tcgtcggcgg tgaccccgaa
    29281 ggcgcagtcg acgagacggt cgaggtccat cgcggttccg gtgaaggtca tcgccgggtg
    29341 cagggccagc ggcagcacgt gctgggcgcg ggccgggtcg aggatgccga ccccgtggcg
    29401 gccgctggtg tgcacgacga tctgcccggc ctgccacacg cccgtggcgg ccagaccggc
    29461 gacgaggtga ccgagggcgt cgtctggcac ggcgagcagg acgagctcgg cgccggcgac
    29521 cactgctgcg acgtccttga cgggcacacc gggcagcagt tctgctgcgc gggcccggct
    29581 ggcctcggag acggcgtagg tgccggtgac ccggtggccc gctctggcga gggctgcccc
    29641 caacacggag ccgactcggc ccgcaccgac gacaccgacg gtgaggcggg cggggtgctc
    29701 gatgccgtcc acgatcgagc acgctaccgg tccgcctcgc tcctttcccg ggttgggtgc
    29761 tcaaatgggc gttcgcgcag gggctgggtg cgccgctgga cgtgcaaaga cgccgtcgcc
    29821 cgccctcgca cccgcgccag tgctgggttt cctgtgcggc gcgccctcgt ttgcacgtcc
    29881 ggcggcgcgg gccagtcatg aacggtgcta gcgagggccg gcgtgcggcg aggtaccgtg
    29941 cgggcgtgat ggtcccgtgg cggcaggcgt ggcacgacgc gctctacgga gagtccgggt
    30001 tctaccgtcg caccgagggg ccggcgggtc acttcacgac gtcgacgcac gggccgctcg
    30061 gggtcgtcct cgccgaggcc atcggccggc tcgccgaccg tgagggctgc atgcacgtcg
    30121 tcgacctcgg ctgtggccgt ggcgaactgc tcacgcacct gcacaccgca cgaccggacc
    30181 tgacgctgac gggggtcgac gtcgtggggc gaccgaacgg catcccggat gccgtggcgt
    30241 ggcttcgctc gccgggcggc ggttcgcttc cggaggggtt gcggggtctg gtcgacgtgc
    30301 tcgtcgtggc ccacgagtgg ctcgatgtcg tgccctgcac cgttgcgcag gtggtggcgc
    30361 cggggcggct cgcggtcgtg ctcgtcgacc cggtgacggg tgaggagggg ctcggcggcc
    30421 cgctcgcggc cgacgacctc gcgtggtgtg cgcggcactg gccggtggac gaccttcccg
    30481 tcggcgcacg ggtcgaggtc ggagggaccc gggatgccgc gtggagcgac ctggtctcgc
    30541 gggttggccg cggcacgctg ctcgccgtcg actacgggca cggccgggga gagcggcccg
    30601 gggagggcac gctgaccggc taccgggcgg gggctcaggt ggcacccgtg cccgacgtgt
    30661 cgtgtgacgt cacggcacac gtcgcggtcg actccctcga ccacgacgag ctgaccaccc
    30721 agcgcgaggc cctgcgcgcg ctcggggtca ccgccgcgtt gcccgagcac ggcctggccc
    30781 gcaccgaccc ggtgggctac gtcgccgccc tgtcgaggtc gagcgcggct gcggcgctga
    30841 tggacccgaa cgggctcggt gcgttccgct gggtgctgcg ccgttgccca ccggccggcg
    30901 cacgccccag tacggcagac tgacgaaggt gacctccgag accacgcccg cgaccggcat
    30961 ccccggcccg acccgcgccc tcgaggtcgg gatgggtgcg ggcgccctcg caacagcgga
    31021 catggtgctc aacatcgggc cccagcaccc ggcgacccac ggcgtgctgc ggctgcgcgt
    31081 ggtgctcgac ggcgagcgca tcgtctcggc cgagccgatc atcggctaca tgcaccgcgg
    31141 cgccgagaag ctgttcgagg tgcgcgacta ccgccagatc accgtgctcg cgaaccggca
    31201 cgactggctc tcggcgttct cgaacgaatt gggcgtggtg ctcggcgtcg aggcgatgct
    31261 cggcatggag gtgcccgagc gcgccgtctg gctgcgcacc ctgctcgccg agctcaaccg
    31321 ggtgctctcg cacctgatgt tcctcggctc ctaccccctc gagctcggcg cgatcacccc
    31381 ggtcttctac gccttccgcg agcgcgagga gctccaggcc gtcatggagg aggcctccgg
    31441 agggcgcatg cactacatgt tcaaccgggt cggggggctc aaggaggacg tgcctgccgg
    31501 gtggctcggg cgggtgtcgg atgccgtggc cgccgtgcgt cgtcggctgc cggatctcga
    31561 gtcgctcatc gtcggaaacg agatcctcga ggccaggacg cggggcgtgg gggtgctcga
    31621 cgtggggacc tgcaccgcct acggcgtctc ggggccgatc gcccgcgcca gtgggctcga
    31681 cgtcgacctg cggcgcgacg ccccctacct ggcctacggc gaactgttcg cacccggcgg
    31741 gccggggcgg gtcgtgacgc gcaccgccgg cgactgcctg gcccgcctgg aggtgctgct
    31801 cgagcaggtg cacgtgagcc tcgatctcgc ggaggcctgc atcgacacct tgcgctcgct
    31861 gccggccggg ccggtcaacc agcgcctgcc caaggtgctc aaggtgcccg agggcgagcg
    31921 ctacaccgcg accgagaacc cgttggggtt caacgggtac tacctggtgt cgcgcgggga
    31981 gaagacgccg tggcggttga agctgcgctc ggcgtcgttc aacaacgtgc aggtgctctc
    32041 gcaggtgctg cccgggtgcc tggtcgccga catggtcgcc gtgctggggt cgatgttctt
    32101 cgtcgtcggg gacgtcgaca agtagccctg cgagtgatgg gcgggcctgc caaccggcaa
    32161 aggttgctcg cagcggggtg agcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32281 nnnagggtca ggtgcgctcg ctcgctcacg acgtcggtgc cggatgccgg gtcgccgtac
    32341 tgcacgaggt cgaggtccag ggtgcgcgcc ccccaccgca cctcgcgcgt gcggtcgaaa
    32401 cccgcctcga cagcgtgcag cgcgcgcagc aacgacgccg gcgagagcgt cgtgcgcgcg
    32461 acgagcacgg cgttggcgta gtcgggctgg ccgggcggac cacccaccgg cgtggtgtcg
    32521 tacgtcggcg acacccggat gccggtcagc cccacgtcga actcgagttc ggcaaccgct
    32581 gcggccaggg tgtcagcggg cgagtcctcg ccacgcacga ggttggctcc cagcgcgatg
    32641 acgacaggca ccggatgccg ttggcgcgtc accgagaccg tcacgtcgcc gaacggcaca
    32701 ccgaccggcg cctgcggctt gtgcaccgtc acggtcacct cgtcgacggc gctgtgcgac
    32761 agcgcatctc cggcgatgac ctcggccagg cgctcgatga ggtcgaaggg ctcaccctcg
    32821 atgcgagcca gcgccgcagc gccgatctcg ccgtagttga cggtgtcgac gaggtcgtca
    32881 ctggccccgg ccgcggcgag gtcgacggtc agcgcgacgt cgacgacgaa ctcctgcccc
    32941 tcgcggcgct cgtgctcgaa gaccccgtgg aacccgcggc cccgcacgcc gctgagcacg
    33001 atgcggtcac tcatgagcgc ctcgcagtgc gtcgacgacg tcgagggtgt cgatggtgct
    33061 gacgacgtcg tgcacccgca ccgcccacac gccgagccgg gccacgtgcg ccgtggtcac
    33121 ggccgtggcg acgtcgcgat cgagaggggg gcggacggca tccggggtgc gcccgacgtg
    33181 cccgaggaag gtcttgcgtg acgtgccgac gagcagtcgg tgcccgagcg ccatgacctc
    33241 gtcgagccgg cgcagcagct cccagttgtg ctcggccttc ttggcgaagc cgaagcccgg
    33301 gtcgaggacg atgttctcgg cggcgactcc ggcaccggtc agggcctcga cgcgcgccat
    33361 cagctcacgc gagacgtcgg ccacgacgtc gtcgtagacg gcgaacgact gcatgaccgt
    33421 gctgtgggcc cgccagtgca tcgcgatgaa cggcacgccg cgctcggcga ccagcgcgcc
    33481 catctcgggg tcggccaggc caccactgac gtcgttgacg acaaccgcgc ccgcgtccag
    33541 cgccgccgcg gcgacctcgg cacgcatcgt gtcgaccgac accggcgcga cctcccgcag
    33601 agcctcgatg accggcagca cccgacgcag ctcctcggca gcgctcggcc gctgcgcacc
    33661 ggggcgggtc gactcacccc ccacgtcgag caggtcggca ccggcgacca cgaggtcgcg
    33721 gccgtgcgcc acggcatcct gcggggtgaa ccaggcccca ccgtcactga acgaatcggg
    33781 ggtcacgttg accacgccca tgacgagggg gcggccgccg cgagcctcgg gcaacgtcga
    33841 cgccgacagc gtcacgagcg ggtcaccacg tcagcgcttg ccgccgagca gcaacgacat
    33901 cgcctcggag cgggtggccg gatcgcgcag ctggccgcgc actgccgagg tgatggtgcg
    33961 cgatcccggc ttctgcacgc cacgcatcga catgcacagg tgctcggcct cgatgaccac
    34021 gatgaccccc tgggccccga ggtgggtcac gagggcgtcg gccacctggg tggtgatctg
    34081 ctcctgcacc tgcgggcggc gggcgaacac ctcgacgagg cggccgagct tgctcagccc
    34141 ggtgaccttg ccgtcctcgg ccgggatgta gccgatgtgc gcgaccccgt ggaagggcag
    34201 caggtggtgc tcgcacgtcg agtacatcgg gatgtcgcgc acgatgatga gttcttcgtg
    34261 gtcgatcgag aaggtcgtcg acagcacgtc ggccgggtcc tggttgaggc cggcatagag
    34321 ctcgcgggcg gcacgagcca cccggcccgg ggtctcgcgc aggccctcgc ggtcggggtc
    34381 ctccccgatg gcgaacagca gctcgcgcac cgcggcctcg gcccgggcga ggtcgacccc
    34441 gcgcgggccg acgccgctcg cgtcagcgcc gacctcagga gtcgacacgg ccaccgtccg
    34501 gcacctggat gatctgggtc ggcggggtgt cctcgacctg cgtcggggcg ccggcgcggg
    34561 ctgccgcgtc gatgtcggcc tccgttcccg ggtcgccggg gttcaccgag gcacccgcgg
    34621 gagcaccgga gccgttgctg cgaccgttga gggcagcctg ctcggccggg gtgagcaccg
    34681 gcgggcggtc cgagagcgtg cggtgctcgc tggagagcca caggtcgcgc accggacgct
    34741 tgcgcacgtc cttgaagatc tcggcgagct cggccgcgtt gagcgtctcc ttgtcgagca
    34801 ggtcgagcac gaggcggtcc aagatgtcgc ggttgtcgtt gacggcgtgc caggcctcgt
    34861 cgtgcgcgtc gtcgacgagc cggcggacct cctcgtcgac gacggcggca acctgctcgg
    34921 agtagtcacg ctggtggccc atgtcgcggc cgaggaacac ctcgccctgg ctctgaccga
    34981 gcttgatcgc accgacccgc tcgctcatgc cgtactcggt gaccatcttg cgggccatgc
    35041 ccgtcgcctt ctcgatgtcg ttcgcggccc cggtcgtcgg gtcgtggaag atgatctcct
    35101 ccgcgacgcg gccaccgagg gcgtaggcga gctggtcgag gatctcgttg cgggtggtgg
    35161 agtacttgtc gtcggtcggc atcaccatcg tgtagccgag agcgcgcccg cgaggcagga
    35221 tcgtcacctt ggtgaccggc gaggtgtggt tcatcgccgc ggccaccagg gcgtgaccgc
    35281 cctcgtggta ggccgtgatc ttgcgctcct tggccgacat gatgcgcgtg cgcttctgcg
    35341 ggccggcgat gacgcggtcg atggcctcgt cgagcacctc gtcgtcgatg agcttcttgt
    35401 caccgcgagc ggtgagcagc gcggcctcgt tgagcacgtt ggccaggtcg gcaccggtga
    35461 agccgggcgt gcggcgggcg acggcgagca ggtcgacgcc ggaggccatc ggcttgccct
    35521 gcgagtgcac ctcgaggatg cggtgacgac cgatcatgtc gggtgcctcg acggcgatct
    35581 gccggtcgaa acggccgggg cgcagcaggg cggggtcgag gatgtcgggg cggttggtcg
    35641 cggcgatgag gatgacgttg gtcttgacgt cgaacccgtc catctcgacg agcagctggt
    35701 tgagggtctg ctcacgctcg tcgtgcccac caccgaggcc tgcgccacgg tgacggccca
    35761 cggcatcgat ctcgtcgacg aacacgatcg ccggggcgtt ggccttggcc tgctcgaaca
    35821 ggtcacgcac gcgggaggca cccacaccga cgaacatctc gacgaagtcg gaaccggaga
    35881 tggagtagaa cgggaccccg gcctcgccag ccaccgcgcg ggccagcagg gtcttgcccg
    35941 tgccgggcgg gccgtagagc agcacgccct tggggatctt ggcgccgacg gcgaggaact
    36001 tggccggctc ggcgaggaac tccttgatct cgtggagctc ctcgactgcc tcgtcggcac
    36061 ctgcgacgtc ggcgaaggtg accttggggg tgtccttggt cgcgagcttg gccttggact
    36121 tgccgaactg catcacccgc gagccgccgc cctgggcctg gctcatgagg aaccagaaca
    36181 ggccgacgag gagcagcacg gggaagaagc tgatgaagat cgtccagaac gcgctggtgc
    36241 tctcgacctt gtcgttgtac ccgttcggca ggttggtgcg cagggagtcc acgagctgct
    36301 cggcgcgggc gtcgacgaac tcggcgcgca ccttggtggc cccgctgacg ttgttcgcct
    36361 cgtcgctgaa ggtctggccg tcgcgcaggt ccagggtgag gacgttctcc gtcgtcatct
    36421 gggccttgtc gaccttcttg tcgacgatga gcttctcggc ctgggccgtg gtgatcgtcg
    36481 tgtagccgcc gtcgccgtcg aaggagaaca ccgtcagcat caccacgacg acggccagca
    36541 cccagaacaa cggggccttg aagatgcgct tgaagtccat gtgggtgcgg agctcgcgct
    36601 ccggtcctcc tgctcgtggg tctttcggcc gccc
//
LOCUS       DAOSOC010000008        33754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1330_length_33754_cov_8.912104, whole genome shotgun sequence.
ACCESSION   DAOSOC010000008 DAOSOC010000000
VERSION     DAOSOC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 33754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33754
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1330_length_33754_cov_8.912104"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PL014_01415"
     CDS             complement(<1..337)
                     /locus_tag="PL014_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55779.1"
                     /translation="MGDERVVEERAVCSGAAVPQADSDAVSPAVLDAVDTPYATPGPD
                     ATPTPDPAVARVEEFRAWLASVDVDELDDAQRVGLITALEGVKGSASAAQARATDAFR
                     CSQEHTRPQD"
     gene            complement(427..1035)
                     /locus_tag="PL014_01420"
     CDS             complement(427..1035)
                     /locus_tag="PL014_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HOT55780.1"
                     /translation="MIDLASSTAGFLAGLTLIVAIGAQNAYVLRQGLRREHVGAVVLV
                     CALSDVVLIIAGVAGIGVLVERAPTALVVVRWLGVAFLTAYGLMSLWRARHPEGLQAA
                     ATTRASRRGVVARAAALTWLNPHVYLDTVVLLGGIAATHGPQGRWWFGAGACVASAVW
                     FVGLGYGARRAHRLLSSARAWQVLEVLIGLTMLAIAVHLARG"
     gene            1106..1969
                     /locus_tag="PL014_01425"
     CDS             1106..1969
                     /locus_tag="PL014_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HOT55781.1"
                     /translation="MDLSTPQLAALVAIADLGTFEAAARHLHVTPSAVSQRIRALESA
                     AGQVLVRRGTPCTPTAAGERLLAVARQTQLLLAEVQTTPSVVDLPLAVNADSLATWWR
                     PVIGEVATWEDVTLRLHVEDQAFSSDLLRRGDVLAAVTSEPQAVQGCAVERLGTMRYL
                     PATSRAFAQRWRTGRGYDWAAMPTVVFNDKDDLQHEVLRRRGLERARIVHRVPTSADF
                     HEAVRLGLGWAAIPEPQLRPDLESGDLVVISARDHVDVELHWQRWRLDSPILDRLTAA
                     VRRAAAAALRR"
     gene            complement(2015..2848)
                     /locus_tag="PL014_01430"
     CDS             complement(2015..2848)
                     /locus_tag="PL014_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT55782.1"
                     /translation="MPEPITDPQDPRLRDYVGLTDVALRRRTEPERGLYVAESEKVIR
                     RALAAGHHPRSFLMGERWLTDLGDVVTAAEANGIPVFVGDPDVIEALTGFHLHRGALA
                     AMQRPELPTVAHVLDGARRVLVLEDIVDHTNVGAVFRSAAALGVDAVLVTPRCADPLY
                     RRAIRVSMGTVFQVPWTRIDPWPGGVSLLRDLGFTSAALALRDDSVGLDELAADPPDR
                     LALILGTEGDGLSHRTLAEVDLTVRIPMLGGVDSLNVAAAGAVAAWALRVPAPVGDAD
                     G"
     assembly_gap    3125..3224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3428..4147)
                     /locus_tag="PL014_01435"
     CDS             complement(3428..4147)
                     /locus_tag="PL014_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT55783.1"
                     /translation="MDWPLVLGLGLAVLAGGVVQSMVGFGMAVVAAPFVVLLAPDLMP
                     VALLVPALALPLLQLAHAERDVAWRPLGWAVAARTLATPVGVAVVALVSPRAIAALVG
                     VLILLTVALSVRTIELRPTPVNAAAAGAVSGVSGTAAAIGGPFLALVLQHERPERVRS
                     TLAVFFLAGSVLGLVGLRAGGQLTAQQVLVGGLWVPFVLLGYAAAVPLRARIDPDRFR
                     RIVLGFCVVASVVVIGRAVLG"
     gene            complement(4147..4920)
                     /locus_tag="PL014_01440"
     CDS             complement(4147..4920)
                     /locus_tag="PL014_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT55784.1"
                     /translation="MPFPDALLVVLAGIGAGTINTIVGSGTLITFPTLLLLGVPPITA
                     NISNNIGLVPGSLTGSIGYRKELVGAAPTLRRLAPMSLLGGVTGAVLLLVLDPKLFRA
                     IVPVLILVGLVLVVTGPRINAWAERRRAEGTAKAANHERAMQGGVFGAGVYGGYFGAA
                     QGIILMGILGSLSSEPIQRLNGYKNVLATIVNGVAALVFIIVAHDQIDWLVVLLIAIG
                     ATIGGVIGSTVGRRLPPPVLRAVIVVIGLVAIVKLVRYP"
     gene            complement(4943..6139)
                     /locus_tag="PL014_01445"
     CDS             complement(4943..6139)
                     /locus_tag="PL014_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT55785.1"
                     /translation="MPDGIFPVVVLLLLAAFAIIVLVRTVRIVPQQTALIIERLGRYS
                     RTLEGGLHILVPFVDLVRANIDLREQVVSFPPQPVITSDNLVVSIDTVIYYQVIEPKS
                     AVYEIANFIQGIEQLTVTTLRNVIGSLDLEQTLTSRDQINGQLRGVLDEATGKWGIRV
                     NRVELKAIDPPQSIQESMEKQMKAERERRAIILTAEGTKASQILTAEGEKQSQILRAE
                     GSAQARILEAQGQARAIQQVFDAIHRGKPTQKLLAYQYLQVLPQIARGDANKMWIIPS
                     ELTDALKGIGGMLGRGQGGFDGGEDDAWVDPGTEGAFAFEETTLEDPAQALATARGQA
                     GLASREAEEHAAPASRVQPPIEPGPDAVPPRPPAPPAPPAPPAPPAPPVADTPPAPPA
                     SPAPPA"
     gene            complement(6151..6618)
                     /locus_tag="PL014_01450"
     CDS             complement(6151..6618)
                     /locus_tag="PL014_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT55786.1"
                     /translation="MEWLTDNFWLAWLGIALVLAAIEAATVDFVFIMFAGGALAGAVA
                     AGLGASFPIQVVVAVATGILLLFTVRPIVKRQFMDGEMDHQIGASSLVGRSARVLQAV
                     TDSDGRIKLAGETWSARLAAGATSTVGPGEEVRVIAIHGATAIVAPVPTSDVQ"
     gene            complement(6691..7473)
                     /locus_tag="PL014_01455"
     CDS             complement(6691..7473)
                     /locus_tag="PL014_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55787.1"
                     /translation="MSDVLALAGVSVVRGDTVILDDVSWEVEEGQRWVVLGPNGAGKT
                     TLLQLASARIHPTSGVVGILGEVLGTVDVFELRPRIGLASAALAERIPPRERVRDVVV
                     TASYGVVGRWREAYDRMDEGRADELLEALGASHLANRHFGTLSEGERKRVQIARALMT
                     DPELMLLDEPAAGLDLGGREDLVRRLGQLAGDLAAPALVLVTHHVEEVPPNFTDVLLV
                     REGRIVAQGPIEITLTEENLEATFGMPLELEVRGDRYAARAR"
     gene            complement(7494..8702)
                     /gene="glgA"
                     /locus_tag="PL014_01460"
     CDS             complement(7494..8702)
                     /gene="glgA"
                     /locus_tag="PL014_01460"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOT55788.1"
                     /translation="MRIDILSKEYPPAIYGGAGVHVTELVSALRARGDLDVRVHAFGH
                     PREEDGTTGHPDLTELTGANAALTTLGVDLSLAAACEGADLVHSHTWYANMGGHLASL
                     MGDIPHVLTAHSLEPMRPWKAEQLGGGYRVSSWVEKTAYEAADAVIAVSAGMREDILR
                     SYPSVDPERVKVVHNGIDSELWQRAVDEDVVRRHGVDPDRPSIIFVGRITRQKGLPYL
                     LRAAAELPPEVQLVLLAGAPDTPEIMAEVESLIETLRETRDGVVWVPTMLPRNEVVAM
                     LSSATVFVCPSVYEPLGIVNLEAMACELPVVATATGGIPEVVVHGETGWLVPIEQVQD
                     GTGTPVDPDRFVADLAAAMTDAVSDAARAERMGLAGRRRAVESFSWGSIGDQTHQVYL
                     DVLRRRQLGS"
     gene            8765..10018
                     /gene="glgC"
                     /locus_tag="PL014_01465"
     CDS             8765..10018
                     /gene="glgC"
                     /locus_tag="PL014_01465"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOT55789.1"
                     /translation="MAYRSGGPKVLVIVLAGGEGKRLMPLTADRAKPAVPFGGIYRLI
                     DFALSNVVNSGYLKIVVLTQYKSHSLDRHVTRTWRMSTMLGNYVAPIPAQQRIDKSWY
                     QGSADAIFQSFNTITDEKPDIVVVVGADHVYRMDFSQMVEQHVETGAGVTVAAIRQPI
                     ETADQFGVIDVVPDDPMKIRAFLEKPTDPDGLPDSPGEILASMGNYVFDADVLMETVR
                     ADATLDGSKHDMGGDIVPALVAQGAAYTYDFKNNVVPGGTERDMGYWRDVGSMDSYYD
                     AHMDLVSIHPVFNLYNFDWPIYTSYGPYPPAKFVHGHHGRFGEALNSAVSPGVVISGA
                     RVNDSVISPDVRVHSYTEIDASVILDSVKIGRHCRIRKTIIDKSVTIPDGTSIGYDKD
                     HDIARGFSVTDSGITVVGKGQVVTP"
     gene            10015..10830
                     /locus_tag="PL014_01470"
     CDS             10015..10830
                     /locus_tag="PL014_01470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT55790.1"
                     /translation="MTAPEHTIRIGAQLQPQHASYADIRRACAELEELGVDVLFNWDH
                     FYPLHGDPDGAHFECWTMLAAWAEATERVEIGALVTCNSYRNPQLLADMARTVDHISD
                     GRLILGIGSGWFERDYDEYGYEFGTAGGRLDALARDLPLIRSRWAQLNPAPTRDIPVL
                     IGGGGERKTLRIVAEHATIWHGFGSPDTIAHKHRVLDEWCARIGRDPNEIERSAGVAP
                     TPGRLPEDVGDYAASAQSLYDVGTRLFTVGLNGPDYDPGPVRDLVAWRDAHAR"
     gene            10853..12079
                     /gene="serB"
                     /locus_tag="PL014_01475"
     CDS             10853..12079
                     /gene="serB"
                     /locus_tag="PL014_01475"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HOT55791.1"
                     /translation="MTEAPVAPQFDADARTLLVTLNGDDRPGVTKELFEAASTVGAEV
                     LDLEQVVVRGHLALSVLLSPGRDENVLVDRITRAGRAAGMRVEIEAGRGDNAPRRTGR
                     AAVVVMGAPLETHQVAAVAATIADHGANIDRIRRLSRFPVTTVELDVSGANLADLRQH
                     LALVSHEVGADIAVAPAGLARRGGRLVVMDVDSTLIQDEVIELLADHAGRAAEVASVT
                     EAAMRGELDFTESLHARVATLAGLPVSVLDDVRDAVRLTPGARTLVRTLKRLGFTVAV
                     VSGGFTEVIEPVARELGIDHVRANRLEVVDGALTGRVLGAVVDRAGKAEALREFAALE
                     GLPLTRTVAIGDGANDLDMLAAAGLGVAFNAKPLVRRQADASLTVPYLDAVLFLLGIS
                     REEIEDADADDTAPLG"
     gene            12084..12941
                     /locus_tag="PL014_01480"
     CDS             12084..12941
                     /locus_tag="PL014_01480"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HOT55792.1"
                     /translation="MPRFPTTIPTLFIIGGAEDRVGKASLLRQFVKLSGGRRSQIVLI
                     PTASSFQQEVTDAYTEVFTRLGAGGLDVVNPATRADAHSPASVAAIDAASGIFMSGGS
                     QLRLSQLLPGTPVGDALHRAHERGAVVGGTSAGASIMSDFMISMGDEGVTPRQRASQI
                     TAGLGLIRGVVVDQHFDQRARYGRLMSVIATSPHLLGIGIDEDTAVIVEDLKRFTVQG
                     RGAVFVVDCRSAVTDAPDAAPGAPVLVSGATVHTLPAGSTFDLVNRALTGFIEQHPDL
                     LVSVAAAKA"
     gene            12994..15831
                     /gene="cphA"
                     /locus_tag="PL014_01485"
     CDS             12994..15831
                     /gene="cphA"
                     /locus_tag="PL014_01485"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOT55793.1"
                     /translation="MDLPTPTGPAAPDLRILESRVYRGGNIWSYDPSIHLVVDLGILE
                     EYPTSALPGFTERLVELLPGLKNHTCSTGVKGGFISRMQQGTWLGHVAEHVALQLQQE
                     AGHDQRRGKTRMVKGRPGVYNIIYAYTNEEVGLAAGRLAVALVNDLVQAQEDFDFAEE
                     LERFLTRAERTAFGPSTGAILEEAVSRDIPFIRLNSGSLVQLGQGVHQQRIRATMTSK
                     TGALAVDIASDKDMTTKLLASAGLPVPKQETVRSAEGAVAAARRIGFPVVLKPLDGNH
                     GRGVCLNLMDDEAVREAFVIAEDQSRRGYVIVESFVTGRDYRCLIVGGKMQAIAERVP
                     AHVVGDGTHTVRELVDLTNADPRRGVGHEKVLTKIKVDAAAEELVREQGFTLDDVPPV
                     ETMVKLALTGNMSTGGISIDRTFDAHPDNIEIAEEAARLIGLDVAGIDFIAPDIASPV
                     REAGGAICEVNAAPGFRMHTHPTVGEPQYIAKPVVDLLFPPGAPSRVPIVAVTGTNGK
                     TTTSRMIAHIMKGLGRQVGMTSTDGIVIDERLVVKSDASGPRSARMVLQNPRVDFAVM
                     EVARGGILREGLGYDRNDVAVVTNVAPDHLGMRGIDTLEQLADVKAVVVEAVPRDGFA
                     VLNADDPLVRRMRRRCSGSVVWFSLAEPGSTVRDLIDDHCRRGGRAVVLDRTDRGDMI
                     VIRHGRRSMQLAWTHLLPATFGGTALFNVANAMAAAGAAFASGAGLHEIRQGLRTFTT
                     SYYLSPGRMNQVNVHNVDVIVDYCHNAPGMRVLGEFLERYASMKSGQSDLGKISRIGM
                     VATAGDRREADMIELGAVAAEHFDVVVVREDERLRGRERGFTADLVAQGVRSRMGEPG
                     VRCRQVEIVLDETDAVRHVMARANPGDIVVLTVDQHAAVMSELEAMTKQAQPGSHTKD
                     SVGDPDMDPEAMMEQAKEAGDSAARDLEPSS"
     gene            complement(15850..18069)
                     /locus_tag="PL014_01490"
     CDS             complement(15850..18069)
                     /locus_tag="PL014_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT55794.1"
                     /translation="MSSEPAQSPPSVRTEEVRLLEGPNLYFPRPAVKVTLALPGYLAA
                     SPDQVRAVAAAVGLRRRGQPGAPGSEQRQQVLVRIVERVARSVAAAAGTKRLGVRTRP
                     AGETDVVVCAFVWRQRGRAQALGEAIGPVLQSLLEGGSLADAVAPHAAAVTSAPRGDG
                     PRVVTPRIPVASITGTNGKTTTTRLLAHICMTAGLTTGWSSTDGVVVQGETIEPGDYS
                     GPAGARAVLDAPGVQIGILETARGGMLLRGMGITHNDVSVVTNVSADHLGLQGIDTVD
                     QLAEVKAIVTKATKPGGWVVLNGEDPRVWAMRAGIRARPWVFTADPSAPAVREALGAG
                     GRATTVLDGHVTVLTHDGVPDRLVRVIDLPMALSGLSHHNVLNALAGASAALGLGVAR
                     EFVIEGLRTFRPDDLLNPGRMNTYTVPTERGEVTVVVDLAHNEAGLEALLDVSRGLCA
                     PGGRVHLALGTAGDRTDEILQALGEIAGLRADHVVAAHKVQYLRGRTAQDLEAQLRIG
                     LGHAGVADIASYPTELSGLQACVEVADDGDVVAVMCHAERAEITQWLRSLSATADDEH
                     AIRRKVVRARGEHEAEEALTALWEVEDPRERIRLAEELAAAHPDDARITYELAGTHDA
                     AGEEDLAVALYERALAAGLREPHRHRAQIQLASSLRNLGQVQAAESVIEDVAARHPES
                     LGVAAFRALVQHDAGHASQALADLLTAVVAASTDPDVARYRRALTASASALRKHPQG"
     gene            complement(18108..>18314)
                     /locus_tag="PL014_01495"
     CDS             complement(18108..>18314)
                     /locus_tag="PL014_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT55795.1"
                     /translation="GPIRTTAAKSIPGFERFEEAWAGRAPLGWDVDDPQPAAQACVAL
                     LSDWFPATTGEMVHVDGGYHAVGA"
     assembly_gap    18315..18414
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18415..18698)
                     /locus_tag="PL014_01500"
     CDS             complement(<18415..18698)
                     /locus_tag="PL014_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT55796.1"
                     /translation="MLLQGKKLLVTGVLLDTSIAFHVARLAQEQGAEVVLTSFGRQMK
                     LTAAMAKRLPVTPAVVELDVTNDDDLAALADRVREHVPHLDGVVHSIGFAP"
     gene            complement(18743..19456)
                     /locus_tag="PL014_01505"
     CDS             complement(18743..19456)
                     /locus_tag="PL014_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HOT55797.1"
                     /translation="MSEPRTVLVTGGNRGIGLAIARSFAAAGDKVAVTHRSGEAPEGL
                     VGVRCDVTDSASVDAAFTEAEGLLGGPIEVLVANAGITKDGLLMRMSDEDFDSVIDTN
                     LAGTFRCARRASKGMIRLRRGRIILISSVVGLYGGPGQANYAASKAGLVGLARSITRE
                     LGGRGITANVVAPGFIETDMTAVLPEDTRKTYQAAIPAGRFATPEEVAAAVRFLASTE
                     AAYISGAVIPVDGGLGMGH"
     gene            19517..19840
                     /locus_tag="PL014_01510"
     CDS             19517..19840
                     /locus_tag="PL014_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HOT55798.1"
                     /translation="MPTTQPRQAPTAQSVTSVPESRTQEQSRRLRRYLVTMVVRTICF
                     VLLVVINHPIRWVFAFGAVFLPFFAVVAANAVSPRLRGRVMPVLPHADDTLLITREAD
                     SPTDR"
     gene            complement(19823..20824)
                     /gene="moaA"
                     /locus_tag="PL014_01515"
     CDS             complement(19823..20824)
                     /gene="moaA"
                     /locus_tag="PL014_01515"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOT55799.1"
                     /translation="MTALPAPRLLDQFGRVGRDLRVSVTDRCNLRCTYCMPAEGLDWM
                     PKPEMLSDDELVRVVSVMVGLGVTQVRLTGGEPILRRSLVEVVARIAALDPRPRIAMT
                     TNGIGLDRLAAPLAAAGLDRVNVSLDTIDPEHFAGLTRRDRFNDVVAGLKAASDAGLN
                     PVKVNAVAMRGVNDTDVPDLLRWCLDRGYELRFIEQMPLDAQHAWDRAQMVSQGEILE
                     RLGERFTLTPVHERGSAPAELFLVDGGPATVGVIASVSAPFCGSCDRVRLTADGQLRN
                     CLFARREVDLRGPLREGATDAELADLIVAEMWAKKAGHGIDRPDFEQPERPMSAIGG"
     gene            20858..21649
                     /locus_tag="PL014_01520"
     CDS             20858..21649
                     /locus_tag="PL014_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOT55800.1"
                     /translation="MFRRLVTPRWLGALVLAALFAVACYHLGWWQYDRHLAKAERNER
                     LDEHYRGEAVPLGEVLTPSGLAVQDEWRRVTVTGTYEAGPVFVRGRSLDGEAGLEVLW
                     GLRPDAGGPDVVVNRGFVRPSDQGASVLPSVPPAPDGEVTVTGWARRGEASRGKEAAA
                     GTVASINLPEVAAQMALTDVLPDYVLLDSERTADGTTPQRPQPLGEPDRSLGTHLAYA
                     YQWWMTMALGFVLIWFGIRRELRAENPQQYPPKVKKTRIWDEEDE"
     gene            complement(21656..23548)
                     /locus_tag="PL014_01525"
     CDS             complement(21656..23548)
                     /locus_tag="PL014_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55801.1"
                     /translation="MYGWQALRSLSRDASVKERRLTPGTTKRVLGYARPYSGPIAWFL
                     GLVVIESVLVVATPLLLKSLIDDGIYPRDSAVVVRLSLIVAGIAVVSGALTLVERWFS
                     ARIGEGLIYDLRTQVFSHVLRQPVAFFTRAQTGALVTRLGNDVIGAQQAFTSVLSSVV
                     SNAITLVLILAAMATLSWQLTLAALVLVPFFLLPARFMGRRLSSLAHEQMVQNADLGT
                     RMTERFNVAGALLVKLFGRPAVEDEEYAVRAARVRDIGVRIAVNRSVFFVALTLVASL
                     ATAMVYGVGGLMAVNGSLTVGTLIALTALLARLYGPLTAISNVRVDIMTALVSFERVF
                     EVLDLEPLVAEAPDARPVPRGPVRVEVDDVAFSYPSADEVSLASLESTASGDRRGSGP
                     VLREVSFVIEPGSLVALVGPSGAGKTTITSLVARLYDPTAGAIRINGVDLRDTTNDSL
                     HDAVGVVTQDAHLFHDTIRANLAYAAPQASEADMVAALKAAQVWNLVESLPSGLETVV
                     GDRGHRLSGGEKQRLAIARLLLKGPGLVVLDEATAHLDSESEQALQRALDEALRDRSA
                     LVIAHRLSTIRHADLILVVDEGRVVERGTHTELVAAGGVYSELYRTQFDDELGEVLDQ
                     PVDTPA"
     gene            23691..25130
                     /locus_tag="PL014_01530"
     CDS             23691..25130
                     /locus_tag="PL014_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOT55802.1"
                     /translation="MTTASPPGRAPERRGDITVTAPLRHKVLLDEDEMPTRWYNILHD
                     LPTPPPPPLHPGTGQPVGPDDLSGIFPMDLIAQEVSTEQYIDIPEAVQDVYRLWRPSP
                     LFRAHRLEKALGTPARIYYKYEGVSPAGSHKPNTAVPQAYYNARAGIRKLTTETGAGQ
                     WGTSLAFACAQFDLECEIWQVRASYDAKPYRKIMMETFGGTVHPSPSDLTEAGRAILA
                     SDPGSNGSLGLAISEAVEVAAQDPNANYALGSVLNHVLLHQTIIGEEALLQLAKIDET
                     VDLIVGCTGGGSNFGGLTFPFLREKFAAGGAGPVIRAVEPASCPSLTQGTYAYDFGDV
                     AGLTPMMKMHTLGHDFMPDPIHAGGLRYHGMSPLISHLYEEGLIEAEARTQKDCFAAG
                     VLFARTEGIVPAPEPTHALAAAVDEAIRCIETGEEKVILTALCGHGHLDLPAYDAYLR
                     GDLVDHTWEDTELQEAVAQALTKLPSIPG"
     gene            complement(25142..26740)
                     /locus_tag="PL014_01535"
     CDS             complement(25142..26740)
                     /locus_tag="PL014_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT55803.1"
                     /translation="MITAASVELRAGSRLLLDDATFRVAPGDRVGLVGRNGAGKTTLT
                     KALAGETQPAAGSITTSGQVGYLPQDPRGVDLTVTARDRILSARGLDEIVRKLRESEL
                     AMATATGEKQDKAMRRYGRLEGEFVAAGGYAAESEAASIAGALGLDERILGQTLGTLS
                     GGQRRRIELARILFSGAETLLLDEPTNHLDADSIAWLRDHLKGYKGGLVVISHDVDLL
                     DAVVNKVFHLDANRAELDQYNLGWKAYLQQRETDERRRKRERANAEKKASVLMAQADK
                     MRAKATKTVAAQNMARRAERLLSGLETERAHDKVAKLRFPKPSPCGKTPLRATGLSRS
                     YGSQEIFTDVDLAIDRGSRVVVLGLNGAGKTTLLRMLAGVDTPDTGQVEPGHGLKIGY
                     YAQEHENLDVDRSVLANMKSAAPDLGETDVRKVLGSFLFSGDDVEKPAGVLSGGEKTR
                     LSLALLVVSSANVLLLDEPTNNLDPASREEILGALRTYEGAVVLVTHDEGAVDALEPE
                     RILLLPDGVEDLFNPDYRDLVTLA"
     gene            26874..27788
                     /locus_tag="PL014_01540"
     CDS             26874..27788
                     /locus_tag="PL014_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOT55804.1"
                     /translation="MSFNENVTLDTSQVSSGGRGGGAPGGMVVGGGIGGIIMLILAMV
                     FGINPSDITGGGDQPAPGQVQSGGEQDAAAFAECVTGADANTNVECRVIGSVNSVQAF
                     WAGELPRFGKQWQPTQTVLYRGTTQSACGTASNQVGPFYCPLDKKVYIDASFFQILEQ
                     QFGSSAGPLAQEYIVAHEYGHALQDQLGLLGRAQQDPQGPESGAVRIELMADCLGGVW
                     ANHASTVKDEDTGQPFLKQLTDQDIKDALSAASSVGDDRIQQKTQGRVNPENWTHGSA
                     EARQRWFLQGFKTGDLNQCDTFAVASVN"
     gene            complement(27793..28041)
                     /locus_tag="PL014_01545"
     CDS             complement(27793..28041)
                     /locus_tag="PL014_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55805.1"
                     /translation="MAAGAAVAAGMSMIWFRIVPDAADDASGLAWVALRLGHGVCWAL
                     LAASMLIGVADGPPRLRAGLAWAALAAYAAFVGALLAG"
     gene            complement(28123..28644)
                     /locus_tag="PL014_01550"
     CDS             complement(28123..28644)
                     /locus_tag="PL014_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acVLRF1 family peptidyl-tRNA hydrolase"
                     /protein_id="HOT55806.1"
                     /translation="MPRRVVEVAPARLDGWLERFHANNPEGPQRIVGQERFDHDPLAV
                     ILVRRGGYAVGVATGGALTEHKVGTRYVQSRTAAGGWSQQRFARRRANQADGLVGAAA
                     EHAVRLLTGIRPRGLVLGGDKALAASLLDDDRLAGLRHLPRRELYDLPDPRLDVLQKA
                     LDRGLAVRITIEE"
     gene            complement(28653..28979)
                     /locus_tag="PL014_01555"
     CDS             complement(28653..28979)
                     /locus_tag="PL014_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HOT55807.1"
                     /translation="MTQTSTPANVADVEEALRDVVDPELGINVVDLGLVYGITVDGQN
                     HAVIDMTLTSAACPLTDVIEDQTQQSLEGLVASYRVNWVWMPPWGPDKITDDGREQLR
                     ALGFNI"
     gene            complement(28976..29422)
                     /locus_tag="PL014_01560"
     CDS             complement(28976..29422)
                     /locus_tag="PL014_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOT55808.1"
                     /translation="MDLYQELILEHSKRPVRFGLREPFEAQVHHVNPTCGDEVTLRVH
                     LAGAGADTVVEDVSYDAVGCSISVASASILAEEITGETLAEARETVEAMRSMLTSKGE
                     DPGDEERIGDGVALSGVAKYPARVKCALLSWMAFTDALHQAEGAPQ"
     gene            complement(29422..30717)
                     /locus_tag="PL014_01565"
     CDS             complement(29422..30717)
                     /locus_tag="PL014_01565"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HOT55809.1"
                     /translation="MDAAPFTDEELARIRADFAILARTVRGGRPLVYLDSGATSHKPR
                     QVLDAERWFYENTNAAVHRGAHQLSEEGTDAYEDARATIASFIGARRSEVVFTRNATE
                     AINLVAYAFTNGAAGGGGGHGDRFTLGPGDEVLVTEMEHHANLIPWQEACRRTGATLR
                     WVPVTPDGRLDLTDLDTLLTERTKVFALTHVSNVLGTVNPVRELTERAHAVGALVVLD
                     ACQSVPHLPFDVSDLGVDFVAFSGHKMFGPLGIGVLWSRPELLDEMPVFLTGGSMIET
                     VTMETSTYAPAPQKFEAGVPVAAQAVGLAAACDYLSALGLERVHAHEQALTRRLLDGL
                     AARPWVRVLGPEGLEDRGGAVAFVVDDVHAHDVGQVLDDSGVAVRVGHHCAWPLHRAL
                     KAQASTRASFAAYTTPAEIDALLAALDRVPAMFGLEVAV"
     gene            complement(30718..31479)
                     /gene="sufC"
                     /locus_tag="PL014_01570"
     CDS             complement(30718..31479)
                     /gene="sufC"
                     /locus_tag="PL014_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOT55810.1"
                     /translation="MSTLEIKDLHVSVTTEDGAKEILRGVTLTINSGETHAIMGPNGS
                     GKSTLAYSIAGHPKYTITSGTVTLDGEDVLAMSVDERARAGIFLAMQYPVEVPGVSMS
                     NFLRTAKTAVDGEAPKLRTWVKDMKQAMENLRMDPSFAERNVNEGFSGGEKKRHEILQ
                     MELLRPKFAVLDETDSGLDVDALRVVSEGVNRAKEATGSGVLLITHYTRILRYIRPDF
                     VHVFVDGRIAEEGGPELAERLEAEGYDRFLTPAEA"
     gene            complement(31487..31828)
                     /locus_tag="PL014_01575"
     CDS             complement(31487..31828)
                     /locus_tag="PL014_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HOT55811.1"
                     /translation="MSEQLTDTDFVRVCRLDELPPVGATLAEINGRRVSLVRTEDGGL
                     HCIDDACTHGNVSLSEGELDGCMIECWLHGSRFDLRTGQPSGPPAIVPVTVHTVRIEG
                     DDVLVALTPES"
     gene            complement(31825..33003)
                     /gene="sufD"
                     /locus_tag="PL014_01580"
     CDS             complement(31825..33003)
                     /gene="sufD"
                     /locus_tag="PL014_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOT55812.1"
                     /translation="MSLLTPQRPASGAHTDSAATMVPDQSRAERTTSYDVADFPLPTG
                     REEEWRFTPVDRIGMLFRDEATGAHLTWSNTLPDGVTLRTASTEEVRDSGAPAPGDRA
                     AVVAAAHSGGATVVEIPAEAELDEPVRLRLHGESSDVVHGHLVVRLGRFARATVVIEH
                     AGTSNYGELVSVVTGDGSQLTLVSVQEWDDSAHHLAQHDVVVGRDASVRHIAVTIGGG
                     IVRMNTNATYAGPGGSFEGLGVYFADAGQHLEHRLFVDHAAPHCRSNVEYKGALQGDT
                     AHTVWVGDVLIRASAEGTDTYELNRNLVLTDGARADSVPNLEIETGEIEGAGHASATG
                     RFDDEQLFYLMARGIPQDEARRLVVHGFFASVVGRIGVAEVEERLMEAIERELSAGAA
                     "
     gene            complement(33003..>33754)
                     /gene="sufB"
                     /locus_tag="PL014_01585"
     CDS             complement(33003..>33754)
                     /gene="sufB"
                     /locus_tag="PL014_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOT55813.1"
                     /translation="IIADEGSYVHYVEGCTAPIYKSDSLHSAVVEIIVKKNARVRYTT
                     IQNWSNNVYNLVTKRATCAEGATMEWIDGNIGSKVTMKYPAVFLLGEHARGETLSIAF
                     AGEGQHQDAGSKMVHAAPNTSSSIVSKSVARGGGRTSYRGLVQILEGAHGSKSSVVCD
                     ALLVDTISRSDTYPYVDIREDDVQMGHEATVSKVSEDQMFYLMSRGMSEEEAMAMIVR
                     GFVEPIAKELPMEYALELNRLIELQMEGAVG"
BASE COUNT         5029 a        11779 c        11950 g         4796 t
ORIGIN      
        1 tgtcctgcgg gcgggtgtgt tcctgcgaac agcggaacgc gtccgtggcc cgggcctgcg
       61 ccgcggacgc ggatcccttg acgccctcca acgcggtgat caggcctacc cgctgcgcat
      121 cgtcgagctc gtcgacgtcc acgctcgcca gccacgcccg aaactcctca acccgcgcga
      181 ccgcgggatc aggcgtgggc gtggcatccg gtccaggcgt ggcgtaaggg gtgtccacgg
      241 catccaatac cgcaggcgag acggcatccg aatcagcctg tggcacagcg gcgcccgagc
      301 acacggcccg ctcctcaacc acccgttcat cccccatgag aaaaccgtac atccgtccac
      361 cgacaccccc tcgaccctgc cccgcatcct gtggacaacg gccgacggcc gctccatcgg
      421 gcgtgctcag ccgcgcgcca ggtggacggc gatcgcgagc atggtcaagc cgatgaggac
      481 ctccagcacc tgccacgcgc gcgccgagga cagcagtcgg tgagcgcgcc gtgccccgta
      541 gccgagcccg acgaaccaca cggcgctggc gacgcacgcg cctgcgccga accaccagcg
      601 accctggggc ccgtgggtcg cggcgatccc accgagcagg accacggtgt cgaggtacac
      661 gtgcgggttc agccaggtga gcgccgcggc acgggcgaca accccgcgac gtgacgcccg
      721 ggttgtcgct gccgcctgca gcccttccgg gtgacgagcc cgccacagcg acatcaggcc
      781 gtacgccgtg aggaacgcga cccccagcca ccgcaccacc acgagcgcag tcggggcccg
      841 ctccacgagc accccgatcc ccgcgacgcc cgcgatgatc agcacgacgt ccgacagggc
      901 acacacgagc accacggcac cgacgtgctc ccgacgtagt ccctgacgca gcacgtaggc
      961 gttctgggca ccgatggcga cgatgagggt gagcccggcg aggaagccgg cggtcgagga
     1021 ggcgaggtcg atcaccagcc agacgctagg cgggagccag catgaaggcc aacgaatgtt
     1081 tctgcacacg cattagcgtt gcttcatgga tctctccacc cctcagctgg ccgccctcgt
     1141 ggcgatcgcc gacctcggca cgttcgaggc cgctgcccgg cacctgcacg tcaccccgag
     1201 cgctgtcagc cagcggatcc gggccctgga gtcggccgcc ggtcaggtgc tggttcgacg
     1261 cggtacgccc tgcactccca ccgccgccgg cgagcgactc ctcgcggtcg cccgacagac
     1321 ccagctgctc ctcgccgagg tgcagaccac cccgagcgtc gtcgacctcc cgctcgccgt
     1381 gaatgccgac tcgctggcca cctggtggcg ccccgtcatc ggggaggtgg ccacgtggga
     1441 ggacgtcacc ctgcggctgc acgtcgagga ccaggccttc tcgagcgacc tgctccgccg
     1501 aggggacgtg ctggcagcgg tgacgagcga gccgcaggcc gtgcaggggt gtgcggtcga
     1561 gcggctcggg acgatgcgct acctgcccgc gacatcccgc gcgttcgcgc agcgctggcg
     1621 caccgggcgc ggctacgact gggccgccat gccgaccgtg gtgttcaacg acaaggacga
     1681 cctccagcac gaggtgctgc gtcgccgtgg gctggagcgc gctcggatcg tccaccgcgt
     1741 ccccacgtca gccgacttcc acgaggcggt gcggctcggg ctgggctggg ccgcgatccc
     1801 cgagccgcag ctgcgcccgg acctcgagag cggcgacctc gtcgtcatca gtgcgcgaga
     1861 ccacgtcgac gtcgagctgc actggcagcg ctggcgcctg gactccccca tcctggatcg
     1921 gctgaccgcg gccgtacgac gagccgcggc tgccgcgttg cggcgctgac accacgcccg
     1981 gatgccgtgg caccggtcgc gagccccacg cacctcaccc gtcggcatcg cccaccgggg
     2041 ccggcacccg tagggcccag gccgccaccg cgccggcggc cgcgacgttg agcgagtcca
     2101 cgcctccgag catcgggatc cgcacggtga ggtcgacctc ggcgagcgtt cgatgactca
     2161 gcccgtcgcc ctcggtgccg aggatcagcg cgagacgatc aggcggatcc gcggcgagct
     2221 cgtcgagacc cactgagtcg tcgcgcagtg ccagggctgc cgaggtgaac ccgaggtcgc
     2281 gcagcagcga cacgccaccg ggccaggggt cgatgcgggt ccacggcacc tggaacaccg
     2341 tgcccatcga gacgcggatc gcgcgccggt agagcgggtc ggcacagcgt ggggtgacga
     2401 gaacggcatc caccccgagg gcggccgcgg accggaacac ggcgccgacg ttggtgtggt
     2461 cgacgatgtc ctcgagcacg agcacccgcc gcgcgccgtc gagcacgtgc gccacggtcg
     2521 gcagctccgg tcgctgcatc gcggccaggg caccacggtg caggtggaag ccggtgagcg
     2581 cctcgatgac gtcaggatca ccgacgaaga cggggatgcc gttcgcctcg gctgcggtca
     2641 cgacgtcacc gaggtcggtg agccagcgct cccccatgag gaaggagcga gggtggtgcc
     2701 cggcggcgag cgcccgtcgg atgaccttct cggactcggc gacgtacagg ccgcgttcgg
     2761 gttcggtcct gcggcgcagg gcgacgtcgg tgagtccgac gtagtcgcga agacgcggat
     2821 cctgcgggtc ggtgatcggc tcgggcaccc gccgatccta gggctgacct gcacgcatac
     2881 cggaccggcc ttctccccag cggcaggcca gaggggagat ggttccaggt ctggatcaag
     2941 gacgacaagc gccgcgaggg cagcgagacg ttcaccgcca ccctgtcgtc cccagcgggc
     3001 ctcaccatcg cggacggcac ggcagacgtc agcatcctcg atgacgcagg aggcctgggg
     3061 tgggggccgg tcgacctcgt ggcagtgctc gtccacgagc tgggcctgga ccacgttgag
     3121 ctcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaccgt ggcggcctgt
     3241 ggcgctgggg tcggcgccgt tgtagcgccc gcggtcgacc gggtcgagag cccagccgtc
     3301 ccgaccccgc gggcccggcc agaggcacct gttgccgcag ccgacctcct cctgctcctg
     3361 gtgctgccgt tcctccgacc cgggtggcgg gtcctggtga cggcgaacgc ggccgatgac
     3421 gcgggactca gcccagcacg gcacgcccga tgacgacgac gctcgccacg acgcagaacc
     3481 cgaggacgat gcgtcggaac cgatcggggt cgatgcgggc ccgcaggggc accgcagcgg
     3541 cgtagccgag cagcacgaac ggcacccaga ggccaccgac gaggacctgc tgcgccgtca
     3601 gctggccccc ggcgcgcagg ccgaccagcc cgagcaccga gcctgcgagg aagaacaccg
     3661 ccagcgtcga gcgcacccgc tcgggccgtt cgtgctggag cacgagcgcc aggaacgggc
     3721 caccgatggc ggccgcggtc ccggacacgc ccgacacggc gccggcggcg gccgcgttca
     3781 ccggggtcgg gcgcagctcg atcgtgcgga ccgacagcgc gaccgtgagc aggatgagca
     3841 cgccgacgag ggcggcgatg gctcgcgggg agacgagcgc gacgacggcg accccgaccg
     3901 gtgtggcgag cgtccgggcg gccacggccc agccgagggg ccgccacgcg acgtcgcgct
     3961 cggcgtgcgc gagctggagc aggggaagcg ccagggcggg cacgagcagg gcgacgggca
     4021 tgaggtcagg ggccagcagg acgacgaagg gggccgcgac gaccgccatc ccgaagccga
     4081 ccatcgactg gacgacaccc ccggcgagca ccgcgaggcc gaggccgagc acgagcggcc
     4141 agtccatcag gggtagcgca cgagcttgac gatggcgacc aggccgatga cgacgatcac
     4201 cgcccgcagc acgggtgggg gcagccgtcg gcccaccgtc gacccgatga cgccgccaat
     4261 ggtggccccg atggcgatga gcagcacgac gagccagtcg atctggtcgt gcgcgacgat
     4321 gatgaagacg agcgctgcga cgccgttgac gatcgtggcg agcacgttct tgtagccgtt
     4381 gagccgctgg atgggctcgc tcgacaggct gccgaggatg cccatcagga tgattccctg
     4441 cgccgccccg aagtagccgc cgtagacccc ggcaccgaag accccaccct gcatggcgcg
     4501 ttcgtggttc gcggccttcg cggtgccctc tgctcgccgg cgctcggccc aggcgttgat
     4561 ccgcgggccg gtgacgacga gaaccagccc gacgaggatc agcaccggca cgatggcccg
     4621 gaacagcttc gggtccagca cgagcaggag gaccgcaccg gtgacgcccc cgagcaggga
     4681 catcggggcc agtcggcgca gggtcggtgc tgcgccgacg agctccttcc ggtagccgat
     4741 gctgccggtc aggctgcccg gtaccaggcc gatgttgttc gagatgttcg cggtgatcgg
     4801 cgggacgccg agcagcagca gcgtcgggaa ggtgatcagc gtgccggacc cgacgatggt
     4861 gttgatcgtg cctgccccga tgcccgcgag gacgacgagc agggcatcgg ggaagggcac
     4921 gagggctggc tgaccgggag aatcaggcgg gcggagcggg ggaggccggc ggcgcgggcg
     4981 gggtgtccgc gaccggaggt gcaggaggag ccggcggggc cggcggggcc ggcggggccg
     5041 gcggccgcgg cgggacggca tccggtcccg gctcgatcgg cggctgaacg cgcgacgccg
     5101 gcgcagcgtg ctcctcggcc tcacggctgg ccagcccggc ctggccgcgc gcggtcgcca
     5161 acgcctgggc ggggtcctcc agggtggtct cctcgaacgc gaaggctccc tccgtcccgg
     5221 ggtcgaccca cgcgtcgtcc tcgccgccgt cgaagccgcc ctggccgcgg ccgagcatgc
     5281 ccccgatgcc cttgagggcg tcggtcagct cgctcgggat gatccacatc ttgttcgcat
     5341 cgccacgggc gatctgcggc agcacctgga ggtactggta ggcgagcagc ttctgcgtcg
     5401 gcttgccccg gtggatggcg tcgaagacct gctggatggc acgcgcctga ccttgggctt
     5461 ccaggatgcg ggcctgggcc gagccctcgg cgcgcaggat ctggctctgc ttctcaccct
     5521 cggcggtgag gatctggctc gccttggtgc cctcggcggt gaggatgatg gcccggcgct
     5581 cgcgctcggc cttcatctgc ttctccatcg actcctggat ggactgcggc gggtcgatcg
     5641 ccttgagctc gacccggttg acccggatgc cccacttgcc ggtggcctcg tcgagcaccc
     5701 cgcgcagctg tccgttgatc tggtcgcggc tggtcagcgt ctgctcgagg tcgagggaac
     5761 cgatgacgtt gcgcagcgtg gtgacggtga gctgctcgat gccctggatg aagttcgcga
     5821 tctcgtacac ggccgacttg ggctcgatca cctggtagta gatgaccgtg tcgatgctga
     5881 cgacgaggtt gtcgctcgtg atgacgggct ggggcgggaa ggacacgacc tgctcgcgca
     5941 ggtcgatgtt ggctcgcacc aggtcgacga acggcacgag gatgtgcagt ccgccctcga
     6001 gggtgcgcga gtagcgaccg agtcgctcga tgatcagcgc ggtctgctgc gggacgatgc
     6061 gcaccgtgcg gacgagcacg atgatcgcga acgccgccag cagcagcagg accaccacgg
     6121 ggaagatgcc gtcaggcatg ggtcaacctt tcactggacg tcagaggtcg gaacaggtgc
     6181 cacgatcgcc gtggcgccgt ggatcgcgat gacacgcact tcctcgccgg ggccgacggt
     6241 cgacgtggca ccggccgcga ggcgggccga ccaggtctcg ccggcgagct tgatccggcc
     6301 gtcgctgtcg gtgaccgcct ggaggacgcg ggctgatcgg cccacgagcg aggacgcccc
     6361 gatctggtgg tccatctcgc cgtccatgaa ctggcgcttg acgatcggcc gcacggtgaa
     6421 gagcagcagg atgccggtgg ccacggcgac gaccacctgg atcgggaacg aggccccgag
     6481 cccggcggca acggcgccgg cgagggcgcc gcccgcgaac atgatgaaga cgaagtcgac
     6541 ggtggccgcc tcgatcgccg cgagcaccag ggcgatgccg agccaggcga gccagaagtt
     6601 gtcggtcagc cactccatgg tgtgacctgc ctttcgtggt gggtgctcac gcgatggacg
     6661 tgaacccggg acggccggtt cccgtcgagg ctaccgagca cgagcggcat agcggtcccc
     6721 ccggacctcg agctccagcg gcatcccgaa cgtggcctcg aggttctcct cggtcagcgt
     6781 gatctcgatc ggcccctgcg cgacgatgcg cccctcgcgc acgaggagca cgtcggtgaa
     6841 gttcggcggc acctcctcga cgtggtgcgt cacgagcacg agggcgggcg cagccaggtc
     6901 ccccgcgagc tggcccaacc gccgcaccag gtcttcccgt cctccgaggt ccaggccggc
     6961 ggcgggctcg tcgagcagca tgagctcggg gtcggtcatc agcgcccgag cgatctggac
     7021 ccgcttgcgc tccccctccg acaacgtgcc gaagtgtcgg ttggcgaggt ggctggcgcc
     7081 cagcgcctcg agcagctcgt cggcgcgtcc ctcgtccatc cggtcgtagg cctcgcgcca
     7141 gcggccgacc acgccgtagg aagcggtgac gacgacgtct cgcacccgct cacgtggcgg
     7201 gatgcgctcg gccagagccg cagaggcgag accgatgcgg ggtcgcagct cgaagacgtc
     7261 gacggtgccg agcacctcac cgagaatgcc gaccacgccg ctggtcgggt ggatgcgggc
     7321 tgacgccagc tggagcaggg tggtcttgcc ggcgccgttg ggtccgagga ccacccaacg
     7381 ctgaccctcc tcgacctccc agctgacgtc gtcgaggatc acggtgtccc cgcgcacgac
     7441 gctcacgccg gccagcgcca ggacatcact catgccctga accctaaccc tcgtcagctg
     7501 ccgagctgcc gccgcctcag cacgtccagg tacacctggt gggtctggtc gccgatcgag
     7561 ccccacgaga aggactccac ggcccggcgt cggccggcca gccccatccg ttcggcgcgg
     7621 gcggcgtcgc tcacggcatc cgtcatggct gcggcgaggt ctgcgacgaa ccggtccggg
     7681 tcgaccgggg tgcccgtacc gtcctggacc tgctcgatcg gcaccaacca accggtctcg
     7741 ccgtgcacga cgacctcggg gatgccgccc gtcgccgtgg cgaccaccgg cagctcgcag
     7801 gccatcgcct cgaggttgac gatgccgagc ggttcgtaga cggaggggca cacgaagacg
     7861 gtggcgctgc tcaacatcgc gacgacctcg ttgcggggca acatcgtcgg cacccacacg
     7921 accccgtccc gggtctcgcg cagggtctcg atgagggatt cgacctcggc catgatctcg
     7981 ggcgtgtcgg gggccccggc gagcaggacg agctgcacct cgggcggcag ctcggccgcg
     8041 gcgcgcagca ggtacggcag gcccttctgg cgggtgatgc gccccacgaa gatgatgctc
     8101 ggccggtcgg ggtcgacccc gtggcgacgc acgacgtcct cgtcgacggc acgttgccag
     8161 agctcggagt cgatgccgtt gtgcacgacc ttgacccgct cgggatccac cgaggggtag
     8221 gagcgcagga tgtcctcgcg cattccggcg ctcaccgcga tcacggcatc ggccgcctcg
     8281 tacgccgtct tctccaccca cgacgagacg cggtagccgc cgccgagctg ttcggccttc
     8341 cacggccgca tcggctcgag gctgtgggca gtcagcacgt gcgggatgtc gcccatgagg
     8401 ctcgcgaggt ggccgcccat gttggcgtac caggtgtgcg agtgcacgag gtcggcaccc
     8461 tcgcacgctg ccgcgaggga gaggtcgacc ccgagggtcg tcagggccgc gttggccccg
     8521 gtcagctcgg tgaggtcggg gtggcccgtg gtgccgtcct cctcgcgagg gtgaccgaag
     8581 gcgtgcacgc gcacgtcgag atcacctcgc gctcgcaagg cgctgacgag ctcggtcacg
     8641 tggacgcccg ctcctccgta gatggcgggt gggtactcct tggacaggat gtcgatgcgc
     8701 acgaatgcca ccctagtgag acacgaggtt cccggcgagg ggtgtttccc ctacggtgtg
     8761 cgacatggcg taccgcagcg gaggtccgaa ggttctggtc atcgttctcg cgggtgggga
     8821 gggaaagcgg ctgatgccgc tcaccgccga ccgagcgaaa ccggcagtgc cgttcggcgg
     8881 catctatcga ctcatcgact tcgcactctc caacgtcgtc aactcgggct acctgaagat
     8941 cgtcgtgctg acccagtaca agtcgcacag cctcgaccgg cacgtcacga ggacctggcg
     9001 catgtcgacg atgctcggca actacgtggc ccccatcccc gcccagcagc gcatcgacaa
     9061 gtcctggtac cagggcagcg cggacgcgat cttccagagc ttcaacacca tcacggacga
     9121 gaagcccgac atcgtcgtcg tcgtcggtgc cgaccacgtc taccgcatgg acttctcgca
     9181 gatggtcgag cagcacgtcg agacgggtgc cggggtcacc gtggccgcga tccgtcagcc
     9241 catcgagacg gctgaccagt tcggcgtcat cgacgtcgtc cccgacgacc cgatgaagat
     9301 ccgcgccttc ctcgagaagc cgaccgaccc cgacggtctg cccgactccc ccggcgagat
     9361 cctcgcgagc atgggcaact acgtcttcga cgcggacgtc ctcatggaga ccgtgagggc
     9421 cgacgcgacc ctcgacggct cgaagcacga catgggcggt gacatcgtcc cggccctggt
     9481 cgcgcagggc gcggcgtaca cctacgactt caagaacaac gtcgtccccg gcggcaccga
     9541 gcgcgacatg ggctactggc gcgacgtcgg gtcgatggac tcgtactacg acgcccacat
     9601 ggacctggtg tccatccacc ccgtgttcaa cctctacaac ttcgactggc cgatctacac
     9661 gtcgtacggg ccctacccgc cggcgaagtt cgtccacggc caccacggcc gcttcggtga
     9721 ggcgctgaac tccgcggtct cccccggtgt cgtcatctcc ggggcgcggg tcaacgactc
     9781 ggtcatctcc cccgacgtgc gcgtccacag ctacaccgag atcgacgcca gcgtcatcct
     9841 cgacagcgtg aagatcggcc gccactgccg gatcaggaag accatcatcg acaagagcgt
     9901 gacgatcccc gatggcacgt cgatcggcta cgacaaggac cacgacatcg cccgtggctt
     9961 ctcggtgacc gactccggca tcaccgtcgt cggcaagggc caggtcgtca ccccgtgacg
    10021 gcaccggagc acacgatccg gatcggggcc cagctccagc cccagcacgc ctcctacgcc
    10081 gacatccggc gtgcctgcgc cgagctcgag gagctcggcg tggacgtgct gttcaactgg
    10141 gaccacttct accccctgca cggtgacccc gacggtgcgc acttcgagtg ctggacgatg
    10201 ctcgccgcct gggccgaggc gaccgagcgg gtcgagatcg gcgccctcgt cacgtgcaac
    10261 agctaccgca acccccagct cttggccgac atggcgcgca ccgtcgacca catcagcgac
    10321 ggccggctga tactcgggat cggatccggg tggttcgaac gggactacga cgagtacggc
    10381 tacgagttcg gcaccgccgg ggggcggctc gacgccctcg cacgcgacct ccccctcatc
    10441 cggtcgcgct gggcgcagct gaacccggct ccgacccggg acatcccggt tctcatcggc
    10501 ggtggcggcg agaggaagac gctgcgcatc gtcgccgagc acgccacgat ctggcacggc
    10561 ttcggctccc ccgacacgat cgcccacaag caccgcgtcc tcgacgagtg gtgcgcccgg
    10621 atcgggcgcg acccaaacga gatcgagcgg tccgcgggcg tggcgcccac gccgggacgg
    10681 ctgcccgagg acgtcggtga ctacgcggca tccgcgcagt ccctgtacga cgtgggcacc
    10741 cgcctgttca ccgtgggcct caacgggccg gactacgacc ccggaccggt ccgtgacctc
    10801 gtcgcatggc gcgacgccca cgctcggtaa cctctgcagg gtgtccctcc ccgtgaccga
    10861 agcgcccgtg gcgccacagt tcgacgccga tgcccgcacc ctgctcgtca ccctcaacgg
    10921 tgacgatcga cccggcgtca ccaaggagct gttcgaggca gcgtcgacgg tgggtgccga
    10981 ggtgctcgac ctcgagcagg tcgtggtccg cgggcacctg gccctgtcgg tgctgctctc
    11041 ccccggccgc gacgagaacg tgctcgtcga ccggatcacc cgagcgggtc gcgcagccgg
    11101 gatgcgggtg gagatcgagg cgggccgcgg ggacaacgcg ccccgccgca ctgggcgtgc
    11161 cgccgtcgtc gtcatgggcg cgcccctgga gacccaccag gttgcggccg tcgccgcgac
    11221 gatcgccgac cacggcgcca acatcgaccg catccgccgg ctctcacggt tcccggtgac
    11281 caccgtcgag ctcgacgtct ccggtgcgaa cctggcagac ctgcgccagc acctcgccct
    11341 ggtctcgcac gaggtgggcg ccgacatcgc cgtggcaccg gccgggctcg cccggcgcgg
    11401 tggccgcctc gtcgtcatgg acgtcgactc caccctcatc caggacgagg tcatcgagct
    11461 cctcgccgac cacgccggcc gggccgcaga ggtcgccagc gtcaccgagg ccgcgatgcg
    11521 cggcgagctc gacttcaccg agagcctgca cgcccgggtc gcgacgctcg cggggctgcc
    11581 cgtctccgtg ctcgatgacg tccgggacgc cgtgcggctc actccgggtg cgcgaaccct
    11641 cgtgcgcacc ctcaagcggc tcgggttcac cgtggcggtc gtctccggcg ggttcaccga
    11701 ggtcatcgag ccggtcgccc gtgagctcgg gatcgatcac gtgcgcgcca accgtctcga
    11761 ggtcgtcgac ggagcgctca ccggtcgcgt cctcggagca gtcgtcgacc gggcgggcaa
    11821 ggcggaggcc ctgcgggagt tcgcggccct cgagggcctg cccctgaccc gcacggtggc
    11881 catcggtgac ggggcgaacg acctcgacat gctggcagcc gccggtctcg gggtcgcctt
    11941 caacgccaag ccgctcgtgc ggcggcaggc cgatgcgtcg ctcaccgttc cctacctgga
    12001 tgccgtgctc ttcctcctcg ggatctcccg agaggagatc gaggatgccg acgcggacga
    12061 caccgcccca ctaggctgac gccgtgccgc gattccccac gaccattccc acgctgttca
    12121 tcatcggtgg tgccgaggac cgcgtcggca aggcctcgtt gctgcgccag ttcgtcaagc
    12181 tctccggggg gcgccgatcc cagatcgtgc tcatccccac cgcctcctcc ttccagcagg
    12241 aggtcaccga cgcctacacc gaggtgttca cccgtctggg tgccgggggg ctcgacgtcg
    12301 tcaaccccgc gacccgggcc gacgcccact ccccggcgtc cgtcgccgcg atcgacgcgg
    12361 catccggcat cttcatgagt gggggcagcc agctgaggtt gtcgcagctg ctgcccggaa
    12421 caccggtggg tgacgccctg caccgtgccc acgagcgagg cgcggtcgtc ggggggacct
    12481 cagcgggtgc ctcgatcatg tccgacttca tgatctccat gggtgacgaa ggggtcacgc
    12541 cccgccagcg ggccagccag atcaccgccg ggctcggcct catccgcggt gtggtcgtcg
    12601 accagcactt cgaccagcga gcccggtacg gccgactgat gtcggtcatc gccacgtccc
    12661 cccacctgct cggcatcggg atcgacgagg acaccgcggt catcgtcgag gacctgaagc
    12721 ggttcaccgt ccagggtcgt ggtgccgtgt tcgtcgtcga ctgtcgcagc gccgtcaccg
    12781 acgctccgga tgccgccccc ggggcgcccg tgctcgtctc cggtgccacg gtccacaccc
    12841 tgcctgccgg gtcgaccttc gacctcgtga accgcgcact caccggtttc atcgagcagc
    12901 accccgacct gctcgtctcg gtcgccgcag caaaggcctg agcaccaccc accaccctgc
    12961 ccgaaacgcc cgcccacccg aggagaagcg ccgatggacc tgcccacgcc gaccggcccc
    13021 gccgcacctg acctgcgcat cctcgagtcg agggtctacc ggggcggcaa catctggtcc
    13081 tacgacccgt cgatccacct cgtcgtcgac ctcggcatcc tcgaggagta cccgacgagc
    13141 gccctgcccg ggttcaccga gcgcctcgtc gagctcctgc ccgggctgaa gaaccacacc
    13201 tgctccaccg gcgtgaaggg gggcttcatc tcccgcatgc agcagggcac ctggctgggt
    13261 cacgtcgccg aacacgtcgc cctgcagctg cagcaggagg ccggccacga ccagcgccgc
    13321 ggcaagacgc gcatggtcaa gggtcgtccc ggcgtctaca acatcatcta cgcgtacacc
    13381 aacgaggagg tcggcctggc cgcgggccgc ctggccgtgg ccctggtcaa cgacctcgtg
    13441 caggcccagg aggacttcga cttcgccgag gagctcgagc gcttcctcac ccgcgccgag
    13501 cgcaccgcct tcggcccgtc gacgggggcc atcctcgagg aggccgtcag ccgcgacatc
    13561 ccgttcatcc ggttgaactc cggcagcctc gtccagctcg gacagggggt ccaccagcag
    13621 cggatccgcg cgacgatgac gtcgaagacc ggggccctgg ccgtcgacat cgccagcgac
    13681 aaggacatga cgaccaagct gctcgcatcg gccgggctgc cggtgcccaa gcaggagacc
    13741 gtgcgctcgg cagagggtgc cgtggcggca gcccgccgga tcggcttccc cgtcgtgctc
    13801 aagccgctgg acggcaacca cggacgaggg gtctgcctga acctcatgga cgacgaggcg
    13861 gtgcgtgagg ccttcgtcat cgccgaggac cagtcgcgcc gcgggtacgt catcgtcgag
    13921 tcgttcgtca ccggtcggga ctaccgctgc ctcatcgtcg ggggcaagat gcaggccatc
    13981 gccgagcggg tgcccgccca cgtcgtcggc gacggcaccc acaccgtgcg cgagctcgtc
    14041 gacctgacca acgcggaccc gcgccgtggg gtcgggcacg agaaggtgct caccaagatc
    14101 aaggtcgacg ccgccgccga ggagctcgtg cgcgagcagg ggttcaccct cgatgacgtg
    14161 cccccggtcg agaccatggt caagctcgcc ctcaccggca acatgtccac gggcggcatc
    14221 tccatcgacc gcaccttcga cgcccacccc gacaacatcg agatcgccga ggaggcagcc
    14281 cggctcatcg ggctcgacgt cgcgggcatc gacttcatcg ccccggacat cgcctccccc
    14341 gtgcgggagg ccggcggtgc gatctgcgag gtgaacgcgg cccccgggtt ccggatgcac
    14401 acccacccga cggtgggtga accccagtac atcgcgaagc cggtcgtcga cctgctcttc
    14461 cccccgggtg ccccctctcg ggtgccgatc gtcgccgtca ccgggaccaa cggcaagacg
    14521 accacctccc ggatgatcgc gcacatcatg aaggggctcg gccgccaggt cggcatgacg
    14581 tcgaccgacg gcatcgtgat cgacgagcgc ctggtggtga agtccgacgc ctcagggccc
    14641 cgctcggccc gcatggtgct ccagaacccg cgcgtggact tcgcggtgat ggaggtcgcg
    14701 cgcggcggca tcctgcgtga aggcctgggg tacgaccgca acgacgtcgc cgtcgtcacc
    14761 aacgtcgctc ccgaccacct cgggatgcgg ggcatcgaca ccctcgaaca gctcgccgac
    14821 gtcaaggccg tcgtcgtcga ggccgtcccg cgtgacggct tcgccgtgct caacgccgac
    14881 gacccgctcg tgcgtcggat gcggcggcgc tgctcgggct cggtggtgtg gttcagcctc
    14941 gccgaacccg ggtcgacggt gcgcgacctc atcgacgacc actgccgccg cggtgggcgc
    15001 gcggtggtcc tggaccgcac cgaccgcggc gacatgatcg tcatccggca cggccgccgg
    15061 tcgatgcagc tcgcctggac ccacctgctg cccgcgacgt ttggtggcac cgcgctgttc
    15121 aacgtcgcca acgcgatggc ggcggccggt gcagccttcg ccagcggagc cggactccac
    15181 gagatccgtc agggcctgag gacgttcacg acgtcctact acctctcccc cggccggatg
    15241 aaccaggtca acgtccacaa cgtcgacgtc atcgtcgact actgccacaa cgcccccggc
    15301 atgcgggtgc tcggagagtt cctcgagcgc tacgcgagca tgaagtccgg gcagagcgac
    15361 ctcggcaaga tctcgcgcat cggcatggtg gcgaccgccg gcgaccgccg tgaggccgac
    15421 atgatcgagc tcggggccgt cgccgccgag cacttcgacg tcgtcgtcgt gcgcgaggac
    15481 gagcgtctgc gcggtcggga gcgcggtttc acggccgacc tcgtcgcgca gggtgtccgc
    15541 agccgcatgg gtgagccggg agtgcgctgc cggcaggtcg agatcgtcct cgacgagacc
    15601 gacgcggtgc gccacgtcat ggcccgggcc aacccgggcg acatcgtcgt cctcaccgtc
    15661 gaccagcacg ccgcggtgat gtccgagctc gaggcgatga ccaagcaggc ccagcccggg
    15721 tcacacacca aggactcggt cggcgacccc gacatggatc ccgaggcgat gatggagcag
    15781 gccaaggagg ccggcgacag cgctgcgcgc gacctcgaac cgagctcgta gcggcgcccg
    15841 gcatccgcct cagccctgcg ggtgcttgcg cagggctgag gcggatgccg tgagggcgcg
    15901 tcggtagcgc gcgacgtccg ggtcggtgct cgccgccacg acggccgtga ggaggtcggc
    15961 cagggcctgc gaggcgtgtc cggcgtcgtg ctggaccagt gcccggaacg ccgccacccc
    16021 gaggctttcc gggtgccgtg ccgcgacgtc ctcgatgacc gactcggcgg cctgcacctg
    16081 cccgaggttg cgcaggctgg aggcgagctg gatctgggcg cggtgccggt gcggctcccg
    16141 caggccagcg gccagggcgc gttcgtacag cgccacggcc aggtcctcct ccccggcggc
    16201 gtcgtgggtc ccggccagct cgtaggtgat gcgggcgtcg tccgggtggg ccgcggccag
    16261 ttcctccgcg agccggattc gttcgcgtgg gtcctcgacc tcccacagcg cggtcagcgc
    16321 ctcctcggcc tcgtgctcgc cgcgggcgcg caccaccttg cgtcggatcg cgtgctcgtc
    16381 gtcggcagtg gccgacagcg accgcagcca ctgcgtgatc tcggcccgtt cggcgtggca
    16441 catcaccgcg acgacgtcgc cgtcgtcggc cacctcgacg cacgcctgga ggccggagag
    16501 ctcggtgggg taggacgcga tgtcagccac cccggcgtgc cccaacccga tccgcagctg
    16561 ggcctcgagg tcctgtgccg tgcgcccgcg caggtactgg accttgtggg cggcgacgac
    16621 gtggtcggcc cgcaggccgg cgatctcacc gagggcctgg aggatctcgt cggtccggtc
    16681 gcccgcggtg cccagggcga ggtgcacgcg cccgccgggt gcgcacaggc cgcgcgagac
    16741 gtcgagcagt gcctcgagac cggcctcgtt gtgggcgagg tcgacgacga ccgtcacctc
    16801 gccgcgctcg gtcggcaccg tgtaggtgtt catccgtccg gggttgagca ggtcgtccgg
    16861 gcggaaggtg cgcagcccct cgatgacgaa ctcgcgcgcg acgcccaggc ccagggccgc
    16921 cgacgcaccg gcgagtgcgt tgagcacgtt gtggtgcgac agcccggaca gggccatcgg
    16981 caggtcgatg acccgcacca gtctgtccgg gactccgtcg tgggtgagca cggtgacgtg
    17041 gccgtcgagc accgtggtgg cccggccgcc ggccccgagg gcctcgcgca ccgcgggggc
    17101 gctcgggtcg gccgtgaaga cccacgggcg ggcccggatg ccggcgcgca tcgcccacac
    17161 ccgggggtcc tcgccgttga gcacgaccca tccccccggc ttggtggcct tggtgacgat
    17221 cgccttgacc tcggcgagct ggtcgaccgt gtcgatgccc tggaggccga ggtggtcggc
    17281 ggagacgttc gtgacgacgg agacgtcgtt gtgcgtgatc cccatcccgc gcaggagcat
    17341 cccgccacgt gcggtctcga ggatgccgat ctgcacgccc ggggcgtcga gcacggcgcg
    17401 cgcgcctgcc gggccggagt agtcccccgg ctcgatggtc tcgccctgga cgaccacgcc
    17461 gtccgtggag ctccaccccg tggtcaggcc ggccgtcatg cagatgtggg ccagcagccg
    17521 ggtcgtcgtc gtcttgccgt tcgtgcccgt gatcgacgcg accgggatgc gcggggtgac
    17581 caccctcggc ccgtcgccgc ggggcgcgga ggtgacggcg gccgcgtggg gagcaacggc
    17641 atccgccagg gaaccaccct cgagcaggct ctggagcacc gggccgatcg cctccccgag
    17701 cgcctgcgcg cgaccacgct ggcgccagac gaaggcgcac acgacgacgt cggtctcgcc
    17761 ggccggccgg gtgcgaaccc cgaggcgctt ggttccggcg gcagcggcca cggaccgggc
    17821 cacccgctcg acgatgcgca cgagcacctg ctggcgttgc tcggagccgg gggcgcccgg
    17881 ctgaccgcgt cggcgcaacc cgacggctgc ggcgacggca cgcacctggt ccggggatgc
    17941 cgcgaggtac cccggcagcg ccagcgtcac cttgacggct ggtcggggga agtacaggtt
    18001 cggcccctcg agcaggcgca cctcctcggt gcgcacggac ggggggctct gcgcgggctc
    18061 gctgctcacg cggggagcct agtggtcgtc agccctctcg ccgaggctca ggcgccgacg
    18121 gcgtggtagc caccgtcgac gtggaccatc tcgccggtcg tggccgggaa ccagtcggag
    18181 agcagcgcga cgcacgcctg ggcggccggc tgcgggtcgt cgacgtccca gccgagcggg
    18241 gcgcggcccg cccacgcctc ctcgaagcgc tcgaagccgg ggatggactt ggcggccgtc
    18301 gtgcggatcg ggccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgcg
    18421 aacccgatcg agtgcacgac gccgtcgagg tggggcacgt gttcgcgcac ccggtcggcc
    18481 agcgcggcga ggtcgtcgtc gttcgtgacg tcgagctcga cgacggccgg ggtcacgggc
    18541 agccgcttgg ccatggccgc cgtgagcttc atctggcggc cgaacgaggt gagcaccacc
    18601 tcggcaccct gctcctgcgc caggcgggcg acgtggaagg cgatggaggt gtcgaggagg
    18661 acaccggtga cgaggagctt cttgccctgg agcagcatct gggaggacct ttcgcggggg
    18721 gtgggtggcg tgctggctcg ggtcagtgac ccatgccgag gccaccgtcg acggggatca
    18781 cggcaccgga gatgtaggcg gcctcggtgc tggccaggaa gcggacggcg gcggccacct
    18841 cctcaggggt ggcgaaacgg ccggcaggga tcgctgcctg gtaggtcttg cgggtgtcct
    18901 ccgggagcac cgcggtcatg tcggtctcga tgaagccggg cgccacgacg ttggcggtga
    18961 tgccgcgtcc gccgagctct cgggtgatcg agcgggccag gccgaccagg cccgccttgc
    19021 tcgccgcgta gttggcctgt cctgggcctc cgtagagccc gacgacgctg gagatgagga
    19081 tgatgcggcc ccggcgcagg cggatcatgc ccttgctggc acggcgggcg caccggaagg
    19141 tgccggcgag gttggtgtcg atgacggagt cgaagtcctc gtcggacatc cgcatgagaa
    19201 ggccgtcctt ggtgatgcca gcattggcga cgagcacctc gatcggccca ccgagcagac
    19261 cctcggcctc ggtgaaggcg gcgtcgaccg aggccgagtc ggtgacgtcg cagcgcaccc
    19321 cgaccagccc ctcaggggcc tctccggacc ggtgggtgac ggcaaccttg tcgccggccg
    19381 cggcgaagga ccgggcgatc gcgagaccga tcccgcggtt gccgccggtg acgaggacag
    19441 ttcgtggctc gctcatgctg tcgaacctag tcgactaccg ctgggtagcc ggagaacagc
    19501 gacatacagt ggacaggtgc cgaccaccca gccccggcag gctcccaccg cccagtcggt
    19561 gacgagcgtg cccgagtcac gcacgcagga gcagtcccgc cggctgcggc gctacctcgt
    19621 gacgatggtc gtccgcacga tctgtttcgt gctgctcgtc gtcatcaacc accccatccg
    19681 gtgggtgttc gccttcgggg ccgtgttcct gccgttcttc gccgtcgtgg cggccaacgc
    19741 tgtctcgccg cggttgcgcg ggcgcgtcat gccggtcctg ccccacgccg acgacaccct
    19801 gctgatcacc cgcgaggcgg attcacccac cgatcgctga catcggtcgc tcgggctgct
    19861 cgaagtccgg tcggtcgatg ccgtgtccgg ccttcttggc ccacatctcg gcgacgatga
    19921 ggtcggccag ctcggcgtcg gtggctccct cgcgaagtgg gccccgcagg tccacctcgc
    19981 ggcgggcgaa caggcagttg cgcagctggc cgtcagcggt gaggcgtacc cggtcacagc
    20041 taccgcagaa cggcgccgag acgctcgcga tgacccccac cgtagccggg ccgccgtcga
    20101 cgaggaacag ctccgccggc gcgctgccgc gttcgtggac gggagtgagg gtgaagcgct
    20161 ccccgagccg ctcgaggatc tcgccctggg agaccatctg cgcccgatcc cacgcgtgct
    20221 gcgcgtcgag tggcatctgc tcgatgaacc gcagctcgta gccccggtcc aggcaccagc
    20281 gcaacaggtc gggcacgtcg gtgtcgttga ccccgcgcat cgcgaccgcg ttgaccttga
    20341 cggggttcag gccggcgtcc gaagccgcct tcagcccggc gacgacgtcg ttgaagcggt
    20401 cccgccgggt caggccggcg aagtgctccg ggtcgatggt gtcgaggctg acgttgacgc
    20461 ggtccaggcc cgcggccgcg agcggcgcgg ccaggcggtc cagcccgatg ccgttcgtcg
    20521 tcatcgcgat gcgcggccgc gggtcgagcg cggcgatgcg cgccacgacc tcgacgaggc
    20581 tgcggcgaag gatcggctct cccccggtga ggcggacctg ggtgaccccc agaccgacca
    20641 tgaccgagac cacgcgcacg agttcgtcgt cgctcagcat ctcgggtttg ggcatccagt
    20701 ccagcccctc ggcgggcatg cagtaggtgc agcgcaggtt gcaccggtcc gtcacggaca
    20761 cgcgcaggtc gcggccgacc cgcccgaatt gatcgagcag gcgcggggca gggagggcgg
    20821 tcataggaca ccaatgtacg acgggtaccg tggctgggtg ttcaggcggc tggtgactcc
    20881 tcgctggctg ggcgctctcg tgctcgcagc cctgttcgcg gtggcgtgct accacctcgg
    20941 gtggtggcag tacgaccggc acctggccaa ggccgaacgc aacgagcggc tcgacgagca
    21001 ctaccgcggc gaggccgtcc cgctgggcga ggtgctgacc ccctccggcc tggccgtcca
    21061 ggacgagtgg cggcgggtca cggtgaccgg gacctacgag gccggcccgg tgttcgtgcg
    21121 cggccgcagc ctcgacggcg aggccggtct cgaggtgctc tggggccttc ggccggatgc
    21181 cggggggccg gacgtcgtgg tcaaccgcgg tttcgtcagg ccgtccgacc aaggagcgag
    21241 cgtgcttccg tcagtgccgc ccgcaccgga cggcgaggtc accgtgacgg gctgggcgcg
    21301 tcgtggggag gcgtctcggg gcaaggaggc ggcggccggc acggtcgcga gcatcaacct
    21361 gcccgaggtg gctgcccaga tggcgctgac ggacgtgctc cccgactacg tgctgctgga
    21421 ctccgagcgc accgcggacg gcacgacgcc acagcgcccc cagcccctcg gtgagcctga
    21481 caggtccctc gggacccacc tggcctacgc gtaccagtgg tggatgacga tggcgctggg
    21541 cttcgtcctc atctggttcg gcatccgccg cgagctgcgg gcggagaacc cgcagcagta
    21601 cccgccgaag gtcaagaaga cccggatctg ggacgaggag gacgagtagc cggtctcagg
    21661 ccggggtgtc cacgggctgg tcgaggacct cgccgagctc gtcgtcgaac tgggtccggt
    21721 agagctcgct gtagacaccg cccgcggcga cgagttcggt gtgggtgccg cgctcgacga
    21781 cgcgtccctc gtcgacgacg aggatgaggt cggcatgccg gatcgtggac aggcggtgcg
    21841 cgatcaccaa ggctgaccgg tcgcgcagcg cctcgtcgag cgcccgttgc agcgcctgct
    21901 ccgactcgct gtccaggtgg gcggtggcct cgtcgaggac gacgaggccc gggcccttca
    21961 gcagcagccg cgcaatcgcc aggcgctgct tctcaccgcc ggacagccgg tggccccggt
    22021 cgccgacgac ggtctccagg ccggagggca gtgactccac gaggttccac acctgggcag
    22081 ccttcagggc ggcgaccatg tcggcctcgc tcgcctgcgg agccgcgtag gcgaggttgg
    22141 cccggatggt gtcgtggaag aggtgggcgt cctgggtgac gacgccgacg gcatcgtgca
    22201 gtgagtcgtt cgtcgtgtcg cgcaggtcga cgccgttgat ccggatggcc ccggccgtgg
    22261 ggtcgtagag ccgcgcgacg agggaggtga tggtcgtctt tcctgcaccg ctcgggccga
    22321 ccagggcgac gagggaaccg ggttcgatga cgaacgagac ctctcgcagc accggaccgc
    22381 tcccccgccg gtctcccgag gccgtgctct ccagcgaggc cagcgacacc tcgtcggccg
    22441 acgggtagga gaaggcgacg tcgtcgacct cgacgcggac gggcccacgg ggcaccggtc
    22501 gggcgtcggg cgcctcggcg acgagcggtt cgaggtcgag gacctcgaag acccgctcga
    22561 aggacacgag ggcggtcatg atgtcgacgc ggacgttgga gatcgccgtg agcgggccgt
    22621 acagccgcgc cagcagggcc gtgagcgcga tcagggtgcc caccgtgagg ctgccgttca
    22681 cggccatgag gccaccgacg ccgtagacca tggcggtcgc gagcgaggcg acgagggtga
    22741 gcgccacgaa gaacaccgag cggttgacgg cgatccgcac gccgatgtcc cgcaccctgg
    22801 ccgcgcgcac cgcgtactcc tcgtcctcga cggcagggcg gccgaagagc ttgacgagca
    22861 gtgccccggc gacgttgaag cgctccgtca tccgggtacc gaggtcggcg ttctgcacca
    22921 tctgctcgtg ggccaggctc gacaggcgac gccccatgaa gcgggcgggc agcaggaaga
    22981 acggcacgag gaccagggcg gccagggtca gctgccagga caacgtggcc atcgcggcga
    23041 ggatgagcac gagggtgatg gcattgctca ccacgctgga cagcaccgac gtgaaggcct
    23101 gctgggcacc gatgacgtcg ttgccgaggc gggtgacgag ggccccggtc tgggcccggg
    23161 tgaagaaggc caccggttgg cgcagcacgt gcgagaacac ctgggtgcgc aggtcgtaga
    23221 tcaggccctc gccgatccgg gcggagaacc accgctcgac gagggtgagc gcgccactca
    23281 ccacggcgat gccggcgacg atgagcgaga gccgcacgac caccgcggag tcgcgcgggt
    23341 agatgccgtc gtcgatgagc gacttcagca gcagcggggt cgcgacgacg agcaccgact
    23401 cgatgacgac gagcccgagg aaccaggcga tcggccccga gtaggggcgg gcgtagccga
    23461 gcacccgctt ggtggtgccg ggggtgaggc ggcgttcctt gacactggcg tccctgctca
    23521 ggctgcgcag cgcctgccag ccgtacatgc tcacggtgcg gttgctcctc gtcgtcagtc
    23581 agccacgcga gagaacgtcg cggccgcaag cagtcaacca ccggcaccct gacgggtgtt
    23641 ccgtgctacg tcgtggaacc cccttgtggt gagcgcggac gccgttcagg atgaccacgg
    23701 cgtcgccgcc ggggcgggcg ccggagagaa ggggagacat caccgtgacc gcaccgctgc
    23761 gccacaaggt cctgctcgac gaggacgaga tgcccacccg ctggtacaac atcctgcacg
    23821 acctgccgac tcctcccccg ccgccgctgc accccggcac cggccagccg gtgggccccg
    23881 acgacctgtc ggggatcttc ccgatggacc tcatcgccca ggaggtgtcc accgagcagt
    23941 acatcgacat ccccgaggcc gttcaggacg tctaccgact gtggcgtccc tctccgctgt
    24001 tccgggccca ccggctcgag aaggccctcg gcacgcccgc tcgcatctac tacaagtacg
    24061 agggggtctc gccagccggc tcgcacaagc cgaacaccgc ggtgccgcag gcgtactaca
    24121 acgcgcgcgc cggcatccgg aagctcacga ccgagaccgg cgccgggcag tggggcacct
    24181 cgctggcgtt cgcgtgcgcc cagttcgacc tcgagtgcga gatctggcag gtgcgggcct
    24241 cctacgacgc caagccttac cgcaagatca tgatggagac cttcggggga acggtgcacc
    24301 cgagcccgtc ggacctcacc gaggcagggc gcgccatcct cgcgagcgac cccggctcga
    24361 acgggtcgct cggtctggcc atcagcgagg ccgtcgaggt ggcggcccag gacccgaacg
    24421 ccaactacgc cctgggctcg gtgctcaacc acgtgctgct gcaccagacg atcatcggcg
    24481 aggaggccct gctccagctg gcgaagatcg acgagaccgt cgacctcatc gtcgggtgca
    24541 ccggtggtgg ctcgaacttc ggtggcctga ccttcccgtt cctgcgggag aagttcgccg
    24601 ccggtggtgc cgggccggtc atccgcgccg tcgagccggc atcctgcccg tccctgacgc
    24661 agggcaccta cgcctacgac ttcggtgacg tcgccgggct gacgcccatg atgaagatgc
    24721 acaccctcgg gcacgacttc atgcccgacc ccatccacgc cgggggtctg cgctaccacg
    24781 gcatgagccc gctcatcagc cacctctacg aggaggggct catcgaggcc gaggccagga
    24841 cgcagaagga ctgcttcgcg gccggggtgc tcttcgcccg caccgagggc atcgtgccgg
    24901 caccggagcc gacccacgcg ttggcggccg cggtcgacga ggcgatccgg tgcatcgaga
    24961 ccggcgagga gaaggtgatc ctcacggccc tctgcggaca cggacacctc gacctcccgg
    25021 cgtacgacgc ctacctgcgc ggtgacctcg tcgaccacac ctgggaggac accgagctcc
    25081 aggaggccgt ggcgcaggcc ctcaccaagc tgccgtcgat cccgggctga tcaggccggg
    25141 ctcaggccag ggtgaccagg tcgcggtagt ccgggttgaa caggtcctcg acaccgtcgg
    25201 gcagcaggag gatgcgctcg ggctccagtg cgtcgaccgc gccctcgtcg tgcgtgacga
    25261 gcacgaccgc gccctcgtac gtgcgcagcg caccgaggat ctcctcgcgc gaggccgggt
    25321 cgaggttgtt cgtcggctcg tcgaggagca gcacgttggc cgacgacacg acgagcagcg
    25381 ccaacgagag ccgggtcttc tcaccaccgg agagcacccc cgccggcttc tcgacgtcgt
    25441 caccgctgaa caggaacgag ccgagcacct tgcgcacgtc ggtctcgccg aggtcaggcg
    25501 ctgccgactt catgttggcg agcaccgacc ggtcgacgtc gaggttctcg tgctcctggg
    25561 cgtagtagcc gatcttcagc ccgtggcccg gctcgacctg ccccgtgtcg ggggtgtcga
    25621 cgccggcgag catgcgcagc agggtggtct tgccggcacc gttgaggccc agcacgacga
    25681 cccgcgagcc gcggtcgatg gcgaggtcga cgtcggtgaa gatctcctgc gagccgtacg
    25741 acctcgagag cccggtggcc cgcagcgggg tcttcccgca gggagagggc ttcgggaagc
    25801 gcagcttggc gaccttgtcg tgggcccgtt cggtctccag acccgagagg agccgctcgg
    25861 cacgacgggc catgttctgc gccgccaccg tcttggtcgc cttggcgcgc atcttgtcgg
    25921 cctgcgccat gagcacggaa gccttcttct cggcgttggc ccgttcccgc ttgcgccgac
    25981 gctcgtcggt ctcgcgctgc tggaggtagg ccttccagcc gaggttgtac tggtcgagct
    26041 ctgctcggtt ggcgtcgagg tggaagacct tgttgaccac cgcgtcgagc aggtcgacgt
    26101 cgtgcgagat gacgacgagg ccacccttgt aacccttgag gtggtcgcgc agccaggcga
    26161 tcgagtcggc gtcgaggtgg ttggtcggct cgtcgagaag cagcgtctcg gctcccgaga
    26221 acaggatccg ggccagctcg atgcggcgtc gctgaccgcc ggagagggtt cccagcgtct
    26281 gtccgaggat gcgctcgtcg aggccgagcg cgccggcgat ggatgccgct tcggactcgg
    26341 ctgcgtagcc tccggcggcc acgaactcac cttcgaggcg gccgtaccgt cgcatggcct
    26401 tgtcctgctt ctcgccggtg gcggtggcca tcgcgagttc ggactcgcgc agcttgcgca
    26461 cgatctcgtc gaggcctcgc gccgacagga tgcggtcgcg ggcggtgacc gtgaggtcga
    26521 ctccccgggg gtcctggggc aggtagccca cctgaccact ggtcgtgatg cttcccgcgg
    26581 ccggctgtgt ctcccccgcc agagccttgg tcagcgtggt cttgccggca ccgttgcggc
    26641 ccacgagccc gacccggtct cccggcgcca cgcggaaggt cgcgtcgtcg agcagcaggc
    26701 gcgagccggc gcgcaactcg acggaggcag cggtgatcac gaaagggccc ttcgagcagg
    26761 tgggggacgt tgcgccggac cggtgcggtt gcgcacgacg gcaggcggtc gccgtctagt
    26821 gtaagtcccg ccgcgtgttg cgcggttcac gcaccggcac ggaggcacga cccatgagct
    26881 tcaacgagaa cgtcaccctc gacacctcac aggtgagctc gggcggtcgg ggtgggggtg
    26941 cgccgggggg catggtcgtc ggcggcggca tcggcggcat catcatgctc atcctcgcga
    27001 tggtcttcgg catcaacccg agcgacatca ccggtggtgg cgaccagccc gcgcccggcc
    27061 aggtccagtc cggcggtgag caggacgcgg cagccttcgc cgaatgcgtc accggtgccg
    27121 acgccaacac gaacgtcgag tgtcgagtca tcgggtcggt caactcggtg caggccttct
    27181 gggccggcga gctcccccgg ttcggcaagc agtggcagcc gacccagacc gtgctctacc
    27241 gcggcacgac gcagtcagcg tgtggcaccg cgagcaacca ggtggggccc ttctactgcc
    27301 cgctggacaa gaaggtctac atcgacgcca gcttcttcca gatcctcgag cagcagttcg
    27361 gcagcagtgc cggccccctg gcacaggagt acatcgtcgc ccacgagtac gggcacgccc
    27421 tgcaggacca gcttgggctg ctcggccgcg cacagcagga cccgcagggc ccggagtccg
    27481 gcgccgttcg catcgaactc atggccgact gcctcggcgg ggtgtgggcc aaccatgcgt
    27541 ccaccgtcaa ggacgaggac accgggcagc ccttcctgaa gcagctgacc gaccaggaca
    27601 tcaaggacgc gctgtcggcc gcctcctcgg tcggcgacga ccgcatccag cagaagaccc
    27661 aaggtcgggt caaccccgag aactggacgc acggctccgc cgaggcccgc cagcggtggt
    27721 tcctccaggg cttcaagacc ggcgacctca accagtgcga caccttcgcg gtggcgtcgg
    27781 tcaactgagc ggtcaaccgg cgagcagcgc ccccacgaag gcggcgtagg cggccagggc
    27841 tgcccaggcc agccctgcac gcagccgcgg tgggccgtcg gcgacaccga tgagcatcga
    27901 agcggcgagg agtgcccagc acactccgtg accgagccgc agcgcgaccc acgccaggcc
    27961 gctggcgtcg tcggcggcat ccgggacgat ccggaaccag atcatggaca tcccggccgc
    28021 gacggcggcg ccggcggcca agccagctcg gcggtgctgc gaccaccacc ggcgcaaagc
    28081 gtgtcgtggg ctcccgaggt tcacccggcc acggtagcgc tgctactcct cgatggtgat
    28141 ccgcacggcc aggccgcgat cgagcgcctt ctgcagcacg tccaggcgtg gatccggcag
    28201 gtcgtagagc tcacgccgcg gcaggtggcg cagcccggcg agccggtcgt cgtccagcaa
    28261 cgaggcggcc agcgccttgt ccccaccgag cacgaggccc cggggacgga tgccggtgag
    28321 caggcgcacc gcgtgttcag ctgctgcccc gacgagcccg tcggcctggt tggcccgccg
    28381 tcgcgcgaag cgctgctggc tccagccgcc cgccgcggtg cgcgactgga cgtagcgggt
    28441 gcccaccttg tgctcggtca gggctccccc ggtggcgacg cccacggcat accccccgcg
    28501 ccggacgagg atcacggcga gcgggtcgtg gtcgaagcgc tcctgcccga cgatccgttg
    28561 cgggccctcg gggttattgg cgtggaaacg ctcgagccag ccgtcgaggc gtgcgggggc
    28621 gacctcgacg actcgtctcg gcaccgggtg cgtcagatgt tgaagccgag ggcgcgcagc
    28681 tgctcgcggc cgtcgtcggt gatcttgtcc gggccccacg gcggcatcca gacccagttc
    28741 acgcggtacg aggcgacgag cccctcgagc gactgctggg tctggtcctc gatgacgtcg
    28801 gtcagcgggc acgcggccga ggtcagggtc atgtcgatga cggcgtggtt ctggccgtcg
    28861 acggtgatcc cgtagacgag gccgaggtcg acgacgttga tgccgagctc ggggtcgacg
    28921 acatcgcgca gggcttcctc gacgtcggcc acgttggctg gggtgctggt ctgggtcact
    28981 gcggggctcc ttcggcctgg tgcaaggcat cggtgaacgc catccatgac aggagggcgc
    29041 acttgactcg ggcggggtac ttcgcgaccc cggagagcgc gacaccgtcg ccgatgcgct
    29101 cctcgtcgcc ggggtcctcc cccttgctgg tgagcatcga gcgcatggcc tcgaccgtct
    29161 ctcgcgcctc ggccagggtc tcgccggtga tctcctcggc gaggatagac gccgaggcca
    29221 cgctgatcga gcagccgacg gcgtcgtatg acacgtcctc gaccaccgtg tcggcacccg
    29281 cgccggccag gtggacccgc agggtcacct cgtcaccgca cgtggggttg acgtggtgca
    29341 cctgcgcctc gaacggctcg cgcaggccga agcgcacggg tcgcttggag tgctcgagga
    29401 tgagttcctg gtagaggtcc atcagaccgc cacctccaga ccgaacatcg caggcacccg
    29461 gtccagggcc gcgaggaggg catcgatctc agcaggcgtg gtgtaggccg cgaagctggc
    29521 gcgagtgctc gcttgggcct tcagcgcgcg gtgcagcggc caggcgcagt ggtggcccac
    29581 ccgtaccgcg acaccgctgt cgtcgagcac ctgcccgacg tcgtgggcgt ggacgtcgtc
    29641 gacgacgaag gccaccgcgc ccccacgatc ctccagtccc tcggggccga gcacgcggac
    29701 ccacgggcgg gccgcgaggc cgtcgaggag cctgcgcgtc agggcctgct catgggcgtg
    29761 cacccgctcg agcccgaggg ccgagaggta gtcgcacgca gcggcgagac cgacggcctg
    29821 agccgccacg ggaacgcccg cctcgaactt ctgcggagcc ggggcgtacg tcgaggtctc
    29881 catggtgacg gtctcgatca tcgacccacc ggtgaggaag accggcatct cgtcgagcag
    29941 ttctggacgg gaccacagca cgccgatacc tagcggcccg aacatcttgt gcccggagaa
    30001 cgccacgaag tcgaccccga ggtcgctcac gtcaaacggc aggtgaggca ccgactggca
    30061 ggcatcgagc acgacgaggg cacccacggc gtgagcccgc tcggtgagct cacgcaccgg
    30121 gttgacggtg ccgaggacgt tggagacgtg cgtcaaggcg aacaccttgg tgcgctcggt
    30181 gagcagggtg tcgaggtcgg tgaggtcgag ccgcccgtca ggagtgaccg gcacccagcg
    30241 cagggtcgcg ccggtgcgcc ggcaggcctc ctgccacggg atgaggttgg cgtggtgctc
    30301 catctcggtg acgaggacct cgtcaccggg gcccaacgtg aaacggtcac cgtgaccgcc
    30361 gccaccaccc gccgcgccgt tggtgaaggc gtacgcgacg aggttgatgg cttcggtggc
    30421 gttgcgcgtg aagacgacct ccgagcgacg ggcaccgatg aacgaggcga tcgtcgcccg
    30481 ggcgtcctcg taggcgtcgg tgccctcctc ggagagctgg tgcgctccgc ggtgaacagc
    30541 cgcattggtg ttctcgtaga accagcgctc ggcgtcgagc acctgccgtg gcttgtgcga
    30601 ggtggcgccg gagtcgaggt agaccagcgg gcgccccccg cgcaccgtgc gcgccaggat
    30661 cgcgaagtca gcccggatcc gggccagctc ctcgtcggtg aagggggcgg cgtccactca
    30721 ggcctcggca ggggtcagga agcggtcgta gccctcggcc tcgaggcgct cggccagctc
    30781 gggcccgccc tcctcggcga tccggccgtc gacgaagacg tggacgaagt cggggcggat
    30841 gtagcgcagg atgcgggtgt agtgcgtgat gagcaggaca ccggaaccgg tggcctcctt
    30901 ggcgcggttg acgccctcgg acaccacgcg cagcgcgtcg acgtcgaggc cggagtcggt
    30961 ctcgtcgagg acggcgaact tggggcggag cagctccatc tggaggatct cgtggcgctt
    31021 cttctcaccg ccggagaagc cctcgttgac gttgcgctcg gcgaaggagg ggtccatgcg
    31081 caggttctcc atcgcctgct tcatgtcctt gacccaggtg cgcagcttgg gcgcctcgcc
    31141 gtcgacggcg gtcttcgcgg tgcgcaggaa gttcgacatc gacaccccgg gaacctcgac
    31201 ggggtactgc atcgcgagga agatgccggc gcgggcccgc tcgtcgacgg acatcgcgag
    31261 gacgtcctcg ccgtcgaggg tcacggtgcc ggaggtgatc gtgtacttgg ggtgtccggc
    31321 gatggaatac gcgagggtgg acttgccgga gccgttgggg cccatgatgg cgtgggtctc
    31381 accgctgttg atggtcagcg tgacgccgcg gaggatctcc ttggcgccgt cctcggtggt
    31441 gacgctgacg tgcaggtcct tgatctcaag ggtcgacatg gggtgctcag ctctcagggg
    31501 tcagggcgac gaggacgtcg tcgccctcga tacggacggt gtggacggtg acggggacga
    31561 tcgcgggcgg cccggagggc tggccggtgc gcaggtcgaa gcgcgagccg tgcagccagc
    31621 actcgatcat gcagccgtcg agctcaccct cggacagcga gacgttgccg tgggtgcagg
    31681 cgtcgtcgat gcagtggagg ccgccgtcct cggtgcgcac caggctcacg cggcgcccgt
    31741 tgatctcggc gagggtggcg cccacggggg gcagctcgtc gaggcggcag acccgcacga
    31801 agtcggtgtc ggtcagctgc tcgctcatgc ggcacccgcc gacagctcgc gctcgatggc
    31861 ctccatgagg cgctcctcga cctcggcgac gccgatgcgg cccacgacgc tcgcgaagaa
    31921 gccgtggacg acgaggcgac gggcctcgtc ctgcgggatg ccacgtgcca tcaggtagaa
    31981 cagctgctcg tcgtcgaagc gaccagtggc cgaggcgtgc ccggcaccct cgatctcgcc
    32041 ggtctcgatc tcgaggttcg gcaccgagtc ggcgcgggcg ccgtcggtga gcacgaggtt
    32101 gcggttcagc tcgtaggtgt cggtgccctc ggcgctggcg cggatcagca cgtcaccgac
    32161 ccacacggtg tgggcggtgt cgccctgcag ggcgcccttg tactcgacgt tgctgcggca
    32221 gtggggcgcg gcgtggtcga cgaacagccg gtgctcgagg tgctgcccgg catccgcgaa
    32281 gtacacgccc aggccctcga aggagccgcc ggggccggcg tacgtggcgt tggtgttcat
    32341 ccgcacgatc ccgccaccga tggtgacggc gatgtggcgc accgaggcgt cccggccgac
    32401 gacgacgtcg tgctgggcga ggtggtgggc ggagtcgtcc cactcctgga cggagaccaa
    32461 ggtcaactgc gagccgtcac cggtgaccac cgagacgagc tcgccgtagt tgctggtgcc
    32521 agcgtgctcg atcacgacgg tggcccgggc gaaccggccg agccgcacga ccaggtggcc
    32581 gtgcacgacg tcggaggact caccgtgcag gcgcagccgc acgggctcgt cgagctcggc
    32641 ctcggccggg atctcgacca ccgtggcacc gccgctgtgg gcggcggcga cgacggccgc
    32701 gcggtcaccc ggcgccgggg cgccgctgtc gcggacctcc tcggtggatg ccgtgcgcag
    32761 cgtgacgccg tcgggaaggg tgttcgacca ggtcaggtgc gcgccggtcg cctcgtcgcg
    32821 gaacagcatc ccgatccggt cgaccggggt gaagcgccac tcctcctcgc gcccggtggg
    32881 cagcgggaag tcggccacgt cgtaggaggt ggtgcgctcg gcgcgggact ggtccgggac
    32941 catcgtggcg gcactgtcgg tgtgcgctcc ggaggcgggg cgctgggggg tcagcagcga
    33001 catcagccga ctgctccttc catctggagc tcgatgaggc ggttgagttc gagggcgtac
    33061 tccatgggga gctccttggc gatcggctcg acgaagccgc gcacgatcat cgccattgcc
    33121 tcctcctcgg acatacctcg ggacatgagg tagaacatct ggtcctcgga caccttcgag
    33181 accgtggcct cgtggcccat ctggacgtcg tcctcgcgga tgtcgacgta ggggtaggtg
    33241 tccgagcggc tgatcgtgtc gacgagcagg gcgtcgcaca cgaccgagct cttgctgccg
    33301 tgcgcgccct cgaggatctg cacgaggccg cggtaggacg tgcggccccc gccccgggcg
    33361 accgacttgc tgacgatcga gctgctcgtg ttcggggcgg cgtgcaccat cttggagccg
    33421 gcgtcctggt gctggccctc gccggcgaag gcgatcgaga gcgtctcacc gcgggcgtgc
    33481 tcgccgagga ggaagactgc ggggtacttc atcgtgacct tggagccgat gttcccgtcg
    33541 atccactcca tggtggcgcc ctcggcgcag gtcgcacgct tggtgacgag gttgtagacg
    33601 ttgttcgacc agttctggat ggtcgtgtag cgcacgcggg cgttcttctt cacgatgatc
    33661 tcgacgacgg ccgagtgcag gctgtcgctc ttgtagatcg gcgcggtgca gccctcgacg
    33721 tagtgaacgt aggagccctc gtcggcgatg atca
//
LOCUS       DAOSOC010000009        29327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1596_length_29327_cov_9.966589, whole genome shotgun sequence.
ACCESSION   DAOSOC010000009 DAOSOC010000000
VERSION     DAOSOC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 29327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29327
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1596_length_29327_cov_9.966589"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="PL014_01590"
     CDS             complement(<1..1259)
                     /locus_tag="PL014_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISPfr2 family transposase"
                     /protein_id="HOT55814.1"
                     /translation="MSDATPPAGFGRPDLTAFARLDGLGLSVTGQRLEPDRAVLACRV
                     VEPDPWCRRCGSEGAARDTVIRRLAHEPLGWRPTVLEVVVRRYRCADCGHVWRQDTSA
                     AAEPRAKLSRTGLRWALEGIVVAHLTVARVAEGLGVAWDTANNAVLAEGKRLLINDPT
                     RFEGVKVIGVDEHVWRHTRRGDKYATVIIDLTPVRDGAGPARLLDMVEGRSKAAFKTW
                     LADRDDAFRDAVEVVAMDGFTGFKTAAAEEIPDAVTVMDPFHVVRLAGDALDRCRRRV
                     QLAIHGHRGFRDDPLYKSRRTLHTGADLLTDKQSDRLRALFVDDAHVEVEATWGVYQR
                     MIAAYRHEDRQRGRELMEKLITDLSAGVPKVLTELTTLGRTLKKRAADVLAYFERPGT
                     SNGPTEALNGRLEHLRGSALGFRNLTN"
     gene            complement(1892..2203)
                     /locus_tag="PL014_01595"
     CDS             complement(1892..2203)
                     /locus_tag="PL014_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOT55815.1"
                     /translation="MSTPASLPYAFVAKIVAADGQHDALADLLAGAVALANEEVGTIV
                     WFAVRTHADTFWIFDAFPDEAARDAHANGAIVAALMANQHLLGAAPEILAADVLASKL
                     P"
     gene            2312..3253
                     /locus_tag="PL014_01600"
     CDS             2312..3253
                     /locus_tag="PL014_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT55816.1"
                     /translation="MRIGLIAIDGCFGSAVASVIDIVRVADGARGDVDPRIDPIELAI
                     LGPKRRVTTTASMTLSVDHPLSESAEFDVVVVPALGTLTAAATNDALQSRDARSVIAS
                     LGRLDDATTRIAAACTGVFAVAETGRMHHRRATTSWFLGPEFLKRYPTVALDLDTMVV
                     VDGNLVTAGAAFAHIDLALSLVRSISPDLAQHVAKLLIIDERPSQAAFVAYEHLRHED
                     PIVVEFERFVRARLDEPFNVAFVAQSLGTSRRTLERRVRAALNLTPLGFVQRLRIERA
                     RHLSATTDLTSAEIALRVGYANAETLRSLLRRERRRS"
     gene            complement(3595..3840)
                     /locus_tag="PL014_01605"
     CDS             complement(3595..3840)
                     /locus_tag="PL014_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55817.1"
                     /translation="MRRSLTLTVPAVAAAAAVALSTAAGPAGATAPPTAAERPTKVVI
                     IVVDALSKEIVETYDMKNVKGLFPDDGVGRPGASVRR"
     gene            complement(3919..4986)
                     /locus_tag="PL014_01610"
     CDS             complement(3919..4986)
                     /locus_tag="PL014_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT55818.1"
                     /translation="MGTLVMIVLLLALTGASLATWFATRPEAGANETGSGAAASDEGA
                     DEQADGEDPASEGDEGADDERRSRSRGRPSVDVDGRRVLHRLAGFAAVGFAVVTVAYT
                     AAMSFTVVPVQQVGIPVTFGTPGSPMNNGIHPKLPWTTVTLMDGTVQIDDNLGDRRTE
                     IRLGNQSIAYVQNNVRWRIRPEAADRLFRDHQTFERIGTALEEPELAAALNAALQDYN
                     PLGTVTGSEKTRDEIATEVRARLNDKLGDRLLIESVTIPVINFDQETQDRINRYQTEI
                     GNTRIAEQRKLTAAAEAAANSILAKSVSDQPNVLVSRCLDTLNEMVSRGQAIPVGFSC
                     WPGGSNAGGVIVDNTTGTTAK"
     gene            complement(5075..6727)
                     /locus_tag="PL014_01615"
     CDS             complement(5075..6727)
                     /locus_tag="PL014_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT55819.1"
                     /translation="MSADLVVKNLAGGHAHRTLFEGLDFTVAPGDVVGVVGANGAGKT
                     TLLRILAGDLAPLEGSVSTAPTDAFVGWLPQEHERMPGETVGEYVARRTGAAAATASM
                     EAAAAALGADEVTRASEDAFAKALDHWQASGAADLDERLSPVLADLGLTVGADALMTS
                     LSGGQAARAALAALLLSRFDVVLLDEPTNDLDLAGLERLEHFVRGLRSGVVLVSHDRE
                     FLSRVVTRVVELDLAQHQVAVYDGGYDAFLAERAVARRHAREAYEEFADKKADLVARA
                     RTQREWSSQGVRNAIKKAPDNDKIRRRAAVESSEKQAQKVRQMESRIARLDEVDEPRK
                     EWALQFTIGSAPRSSSVVATLSDASVTLGTFTFGPASVQVSAGDRIGVTGPNGAGKTT
                     LLRLLLGHLTPDAGRASLGASVAVGEIDQSRTGLAEDRPLGDAFEAAMPQMTPADVRT
                     LLAKFGLKADQVTSLVRALSPGERTRAAMALLQARGVNLLVLDEPTNHLDLPAIEQLE
                     QALDSYDGSLLLVSHDRRMLDTVRLDQRWHVEDGQVTVRQTG"
     gene            6778..6981
                     /locus_tag="PL014_01620"
     CDS             6778..6981
                     /locus_tag="PL014_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOT55820.1"
                     /translation="MAMTLRLTDEDERALAALAAAQGISKQEATVRAIRDAAARGLHE
                     AKVSELSAQARERYADVLDRLGR"
     gene            6978..7340
                     /locus_tag="PL014_01625"
     CDS             6978..7340
                     /locus_tag="PL014_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HOT55821.1"
                     /translation="MTVYLSLEDLIALTRDLDVGPVRDLGLLDAAAHRPRTELYGQQA
                     YPDLDTKAAVLLDSIVRNHALVDGNKRLGWLATVVFYGINDVDLDAPDDDAYELVMAL
                     ARSESTVEELAAALRSWH"
     gene            complement(7470..9602)
                     /locus_tag="PL014_01630"
     CDS             complement(7470..9602)
                     /locus_tag="PL014_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HOT55822.1"
                     /translation="MSFADPIRRNPRIPLDPRFPAVPTDLDPQDPGKGRRRNRCGTME
                     EHRQLVRLDPEYRWRREQIEKDVQQWIAAFGEEGSRTGIIRIPVVVHVIWHTAAENIS
                     DAQIHSQIDVLNADFRRLNADAGSVPSAFAGVAADARVEFALAVRGPGCAVTTGITRT
                     ETSVTGWTRNQTGMLSAAGGGHDPWDVTRYLNVWVCNYTDGLLGKGSFPGMPNQGVRC
                     HYRAFGSTGTLSAPYDLGRTMVHEVGHYLNLNHIWGDDGSLCTGTDNVADTPNQADEH
                     YGTPAFPQVSCSNGPDGDMFMNYMDYTDDAGMFMFTAGQSERMDATLHTARTGLLASD
                     GLVPSSGAVNDLWAKDTSDDDGAEPNPSGQPMWVSDDIWVRNDNNGIANQDHQNPEHG
                     SGTTSVYVRVRNRGCSGTSSGTVRLYWAKASTGLAWPAPWDGSVTSPALMGGEIGSAA
                     VSVSAGDDEILAFPWSPPDPADYASFGADRGHFCLLARIETGPAPDFGMTSPETGNLY
                     ANVQQNNNIVWKNITVVDEDTDGRVSAAIVANYGREATKVHVVFRAPRRERSMLDWGE
                     VWLEPSDELRELWKASGGESQGLEEIGDGRFRIAGRKASLGPVALEPGQLGALGLRFV
                     PTHRERLGAGIVALDVWQLQADRGEPVGGQRFVVKLRADRRGLVLDQPKVRHDGIGWI
                     RPRSANRLGSLMGRVLGGGGRIRGTTRT"
     assembly_gap    9677..9776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9886..10515
                     /locus_tag="PL014_01635"
     CDS             9886..10515
                     /locus_tag="PL014_01635"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT55823.1"
                     /translation="MSIRLGDIAPDFTADTTQGVLSFHDWKEGAWAVLFSHPADFTPV
                     CTTELGRTAALSGEFAARNAKAIAVSVDSVEDHLRWVPDIAEVAGVDVDFPIIADEDR
                     SVSQAYDMIHPGEGDTSTVRSVFLIDPSNTVRLTITYPKSVGRNFTEILRALDALQAT
                     DRSPISTPAEWVPGDRVIVAPAIATDDARERFANVDEVKPYLRFADAPA"
     gene            complement(10542..10847)
                     /locus_tag="PL014_01640"
     CDS             complement(10542..10847)
                     /locus_tag="PL014_01640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT55824.1"
                     /translation="MADPAEQVVSRNEDESRWEAHVGGVLAGFAAYQVVDDDVVFTHT
                     EVDPAFEGRGVGGALARGALDEVAQDDSLRVVPRCPFIKAWIARHPDYAALVRPTPP"
     gene            10954..12663
                     /locus_tag="PL014_01645"
     CDS             10954..12663
                     /locus_tag="PL014_01645"
                     /inference="COORDINATES: protein motif:HMM:NF021588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HOT55825.1"
                     /translation="MNPAIVEKAARRVGRSPFLPAIGDADRVFERVLSGVGLMVVPWA
                     FAASLVPSLLPRAPWLQGVASGVTVAVGYGLGAAAHALWRYLQIPGLKGRARSIALGL
                     VIGIGMVLVLLSAWKFVGWQNDIRRIMRMESTSPTIWPVVIVVTLLVFTLFVLLARSL
                     IVLARTVASWLGRRLSRRLSITLGVAAVLFLLWGLWTGVIVNGFFAGANAFFAPQDTT
                     GTNSSNRPTAPERSGSPESLSPWEDLGQYGRFFVRGGPDVAELEAVNGPGAKEPIRVF
                     AGLQSADTVQGRADLVLEELKRTGAFDREVLVVATTTGMGYLDHRGTDPLEYLWNGDT
                     AIAGVQYSYLPSWISMLADQDAVASTSRVVFETVHQYWSTLPSNDRPDLYLYGLSLGS
                     RGVESVLSSISIVNAPVNGALMSGPPFVNPLHARLQAERDPDSPASLPIYEDGRTVRF
                     ANSEGGLERGGARWGPTRLVYLQYASDPTVFYSPNLAFREPDWLKEGQRGPDVAPTME
                     WFPFVTMWQVLLDMPAGTAVPAGFGHMYSSQDNLDSWAALTQPPGWGPGMRERLSTAL
                     PSD"
     gene            12718..13629
                     /locus_tag="PL014_01650"
     CDS             12718..13629
                     /locus_tag="PL014_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HOT55826.1"
                     /translation="MSAPAGWHLQPDGRERFWDGTQWTDQFRSPAPSDPTAPPPAPSW
                     ASSSDTVADTVSDSGHTQAFDVAGTQALPRQDAGTPGAYPTAGQAPAGYPQQGYAPGQ
                     PQTGYGAPGAAGYPPPVKSGNSGLAKGCLIAAVVILGLIAIVIAVFAFFASRVVDTVN
                     ETFPSGFPTSLPSGFPTELPTDLPSGLPTDLPSGLPTDIEQNIELDVGDGFELPRATI
                     GGGWTLVPQGAGIPIVNITGMKATLGDANGFPVLFTMRFPAAGGESVETVCTAPAGEP
                     DAVVDVSCVPLFGDVAEATTVTVTASL"
     gene            complement(13725..15080)
                     /locus_tag="PL014_01655"
     CDS             complement(13725..15080)
                     /locus_tag="PL014_01655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT55827.1"
                     /translation="MRLALRIVAALLVLVIAAAVGGYFYARPLLETGTGYAAHNSCAV
                     TLVAGRDDPETDLPDNPLVPFLTGYVNEAGRSSTSQILFTLSTQKAWYTRGFGCTVGA
                     DRPEDLGEATKVDHDTNPLSTAESPEPNPDVDAAIARAFGDGLGTVRAAELGTRGIVV
                     LKGGQLIGERYAEGFTRETPQLGWSMTKSVANLLVGRMVHQERIAITDDSLLPEWTDQ
                     RKGITVDQLMRGTSGLSWDETYSLGTPITQMLYSEPDMAGFAASQPLAHQPGTYQQYS
                     SGGTNILCAVLTDLADAPDADLPRQQLFAPLGLTSATLEVDMTGTPVCSSYLWATPRD
                     WATIGQFALQDGVWAGERLLPEGWMKASTTVTEVRQSEEQGYAAGWWANKKADGSLVD
                     PQLPEDTYWASGHDGQRVYVVPSAELVVVRLGFTPELEADQLRTDELVAELVAIKPAE
                     G"
     gene            15695..16636
                     /locus_tag="PL014_01660"
     CDS             15695..16636
                     /locus_tag="PL014_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HOT55828.1"
                     /translation="MAGGLVALLDDIAALAKLAAASVDDVGAAAGRASAKAAGVVIDD
                     TAVTPRYVDGLKPERELPIIRKIAMGSLRNKLLIILPAILLLSQFLPWSLTPILMVGG
                     TYLAYEGAEKIWEMVAGHEHAVEAVAAGDPDHEKTVVSGAVRTDFILSAEIMVIALNE
                     VADEAFVSRAVILALVAVGITILVYGVVGLIVKMDDIGLHLAGRESGASQRVGRGLVA
                     FMPKLLAGLSTVGIVAMLWVGGHILLVGVHDLGWHWLYDLVHHAEEAVGGGFPGWLVN
                     TLASAVIGLAVGALVVLVVTQVKRMRSPEGDHAASAH"
     gene            complement(16630..17283)
                     /locus_tag="PL014_01665"
     CDS             complement(16630..17283)
                     /locus_tag="PL014_01665"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HOT55829.1"
                     /translation="MSTRAARLRLLGLVLSVAIASALAWVVIGGDLDTVQSTVESTGA
                     WGPVVYVALHVLLTLVPVSKNLLAGVAGALFGLVGGIALSWVASMISAAVTFAIARRL
                     GRTAVASMTGPRIDRVEEIMRQQGLLAVIIARVTPVIPFTIVNYGAGITAVTSRDFVL
                     GTAIGILPGTVGYAALGASAGRDATTFVLAGVMAVALFAGSLFLGWRATRRQQRSAQ"
     assembly_gap    17349..17448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17688..19097)
                     /locus_tag="PL014_01670"
     CDS             complement(17688..19097)
                     /locus_tag="PL014_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT55830.1"
                     /translation="MTTNDHSPTPPNPAGPGARSTSGMRQRVTDAGRTVGRGGRAGIR
                     AVGTGGRAAGRRFRSFASADGAADTGLARLTELHAVNVAADAALTVSLAGTVFALPTD
                     EARSRVALFLVLTMAPFVLLAPLIGPLLDRFRHGRRWALGTTLATRAFLAWVLASAVV
                     EDSAWLFPAALGCLVASRSYAVARAAAVPRLLPDGITLVTANARLNIAGLVGMALGGG
                     LAAALSRIGPDWSLRLAFAIYVSATVLAIRLPARVDSTTGEVDVDGIPVSEAPLPTLE
                     GGSRRLRALPPLVRFTLWVTCGARVLSGFLTLFLAFLMREHPIPGWSGPVVLALVAGA
                     AGAGNALGSVIGNRRRTPPPERMATAIALVALSAALATSILYSVWALVLLGLATGLFG
                     QLAKLCLDALIQRDIADDVRARVFSWSETILQGFWVLGGAIGIAIPLRPGLGFGLVAV
                     VVALLGAVAIRSRHLGSGR"
     assembly_gap    19228..19327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19435..19830
                     /locus_tag="PL014_01675"
     CDS             19435..19830
                     /locus_tag="PL014_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT55831.1"
                     /translation="MNTPMSMAERYTQARGLFEHGEYQAAADSLRALVDESALEPPLH
                     GTTELRVLLGRAYFHSAQLGRAETVLRTVLIEYPDDAYANLVLGRTLQRLGRAADARP
                     HLAMAELLGGYAGTGRLPADTSDPTDSSA"
     gene            19827..21464
                     /locus_tag="PL014_01680"
     CDS             19827..21464
                     /locus_tag="PL014_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT55832.1"
                     /translation="MSDYGHDLEFGLFPTPDAARPHRVLELATLAEVSGLDLVSVQDH
                     PYQARHLDAWTLLSVIAARTSTVRVALNVANLPLRNPVVLARSAASLDLLTGGRVELG
                     LGTGAFWDAIEAAGGTRLTPGEAVTALEEAIAITRALWAGEGSVRVDGEHHRARGLHS
                     GPAPAHPMGIWVGAYKPRMLRLTGRLADGWLPSQGYAAPETLGPMNARIDEAALAAGR
                     SPEAVRRLYNVSGTFGSGAGFLRGQPRTWAEQLAELTLEQGMATYILATDDPDDVRRF
                     GDEVAPAVRELVAAERARRATQPSEAVARDAVATSPSRVEASASSVEPSAGAPRVSSG
                     APAGGRENRPVVTPTPDDGRRLTGILPWDEASRPTYPRRPDAQEQAGYGPAQLAVPQH
                     LIDVHNHLRSELTQLRDVVDQVVRGHLQVGAARSAVNAMTMRQNNWTLGAYCESYCRI
                     VTGHHSLEDAAIFPHLRAQDPDARPVIDRLEDEHEVIHDVLDDVDRALVALVADEPGA
                     LDRLRHVVDLLTDTLLSHLAYEERELLHPLARHGMTR"
     gene            complement(21468..22055)
                     /locus_tag="PL014_01685"
     CDS             complement(21468..22055)
                     /locus_tag="PL014_01685"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOT55833.1"
                     /translation="MTNPFTTFDPPLAGPPVLEPTDVSYVLEDTAVGRLLIAVRSTGA
                     VVTCAYSPDTASEDRWLTRIAGTVSPRVLRHPSPTDAVRRALAAYLAGGARSVDVPTD
                     LALATPFQREVLTGLAARVGYGERTTYGELASAVEHPGAARAVGTALGGNPLCIVLPC
                     HRVLPASGGVGGYAGGAAAKEHLLALEAEHVRPPA"
     gene            complement(22052..22528)
                     /locus_tag="PL014_01690"
     CDS             complement(22052..22528)
                     /locus_tag="PL014_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT55834.1"
                     /translation="MSLPPFQHLVDEHWRDVARLAHGLAPGHGDDVAQQAWTQALAAY
                     PRLTHARNLRSWLLTITHRCAMDHHRTARRTTPHDDLSLLDQAPAHLPPEVPDGDLWA
                     RVAALPQRQRDAVVLRFVGDLDHRAVAAALGTSPGMSRRLVSDAIATLRLDLRDQP"
     gene            22698..23540
                     /locus_tag="PL014_01695"
     CDS             22698..23540
                     /locus_tag="PL014_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT55835.1"
                     /translation="MEVVSEGLSKPEPRRGGPLVIAHRGASDEEPEHTLAAYRRAFAH
                     GADGVECDVRLTADHHLVCVHDRRIERTSNGRGVVSALELALLEELDWASWRTAHRPG
                     DTELPEADPERGHLLTLRRLLDTLVDTGQEVVTLIETKHPTRHGSDVEKALVHVLRAF
                     DLAGGDLPGRPHVRMMSFSHTALQRAHRLAPKLPLVYLVDATRPRMTWDGILPAGIGA
                     VGLDVRMLRNPRAVRAFQRRGHQVYVWTVDTDDDITRCLDLGVDVIITNRPAHVLDRI
                     AASG"
     gene            23550..24083
                     /locus_tag="PL014_01700"
     CDS             23550..24083
                     /locus_tag="PL014_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT55836.1"
                     /translation="MTPPPPHSSHSSADDDDSAEVGSRRTGESGLVVVESSGPGVDSI
                     ICTVRPPHDPSSVGRIRREIVADLQAREMPDELVDEAELVASELVTNALRHARPLSDG
                     TIRVRWKIRGEVVEVEVTDAGGDTVPHPAPRTVWLSSGRGLRIVRSIAHEWGVTEDRT
                     GTVVWATLGGPSRRRAN"
     gene            24193..25083
                     /locus_tag="PL014_01705"
     CDS             24193..25083
                     /locus_tag="PL014_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5926 family protein"
                     /protein_id="HOT55837.1"
                     /translation="MGKASRRQRGTERGTARSRPGPVPFERRPFEGLAGETDWVAMRE
                     ILPAATATVSLRPEHVPDGAPTEVTVATVLPMAWPGLHRADGTVFVGTQSGNASGDAS
                     RDLAGQVLAALAAEPGNPVVTTAPATADSPRLQDLLTDDTTFEVTVHEGFDFWVEGQE
                     LEGDAAASLERANETVVPTTKMAALPSAYWVRIGDRAHIRLVLPDDEDAATDALARMH
                     ASGDDALGGDTRLLGAFRACGLLVPVWDLDPELGADDYEDELTAWSTRYAAALASTES
                     LSADERRARSGLLSRQVTLR"
     gene            25083..25814
                     /locus_tag="PL014_01710"
     CDS             25083..25814
                     /locus_tag="PL014_01710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT55838.1"
                     /translation="MSALAAQVSAVIPCKDEADRIAATVRAVAALPQVGRVVVVDDGS
                     TDDTSRVAREAGADVVRHERNRGKAAALETGARRVRELERADAAAPWRSALLFVDGDL
                     QETAANLGVLTTAVLDGTADMTIATLPAQITAGGGRGLVVNLARRGIRHLTGFTAVQP
                     LSGMRCVGPRAVDAATPLARGWGVETALTVDVLRAGLTVLEVPCELQHRVSGSDWRGQ
                     LHRAGQYRDVWLALVARGWRPWHRD"
     gene            25799..27319
                     /locus_tag="PL014_01715"
     CDS             25799..27319
                     /locus_tag="PL014_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55839.1"
                     /translation="MAPGLTRAHRLCVGLAVALLLLVGASRPNAATPDLSPSGWGPGT
                     LVPITLGPGAVTGVLWTAYLVGGLAVLLGLRAGVGALRTWALPVGLGALALLTAPFGS
                     GDHLNYLAYGRILVGGGNPWVESPIDWAGGADPVTSRVEAPWTTEPSVYGPFGALLHG
                     LAALLGGDSLRQGVWVWQVLVVGAWLGVRWVLRRVLDPSAHGRVDVLWTLNPLVFGVG
                     VLGAHIDLIAAALAVVAVGVVVARPGVVGAVLGGGVVALAGSTKFTYAVVGAGIVAAW
                     WLVGLRGGGLVRRVVALGGGFVLVAGVLHAWAGPHVYDQLMRSRQAVSLATPWRPLLE
                     WGRDVWGNEQTRVAISLGAAVLAVLLAWCLLRLSRPAARPWRDLLADGGTDAVAPPSD
                     AVAPVALWVTACLSLAFSLAAPYSLPWYDLLVWAALPALLPGLVDLVALVRLTALSIA
                     YVPGRVLGMTPQVEELTLGVRREVVPWVGVALWVLVIVAGVRSGSALRRGPRRAGT"
     gene            complement(27255..>29327)
                     /locus_tag="PL014_01720"
     CDS             complement(27255..>29327)
                     /locus_tag="PL014_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55840.1"
                     /translation="PAGAVVLVGTGGISWSDVDETTTPNLWVLLRDGASAALVARSVN
                     SNTCPVDGWLGISSGSRAAAERTGDADNPANRPCPPPPTVSPEGAVSTWSDYVSAAAD
                     RRFVADLGLLGDTAAAGGRCVRAVDPLAAVGGATAAGTVEQYSTWSPDTLLEDLNACP
                     VTLVDVGSVRDPDDVAPGEVAPGSRADQVAAIDARIGEVIAAGPNGAEYIVASLSDAG
                     TSERLRLVIARGPHFGPGVLVSGSTKQEGLAQIPDITATVLASVGLPVPDAVNGAPMT
                     VKSAPDNSQQRAQQRLTTLRDYDEASHDVHSLVEPFFTVFAYGQLVIYLIVLLVWKGR
                     LGTEASRVKVLSRVRVLAVAAASVPVSTFLANLVPWWRFPIEMVAVVAVVGVFVALIA
                     TAALRGPWRGFALGPMAFVSAVTMLVLAVDIMTGARLQLSSLMGLQPVVGGRFYGMGN
                     TTFALFATATILLCTAVSSWLVLQNRRRAAAIAVAAIGGGATVINAMPFWGADGGGPP
                     ALIPGIIYLVFAVLGIAMTWKRVLLIGGSVVALFFLIGVADWLRPAASRSHLGRFIQS
                     IVDGNALDVVIRKGEQNLGILLGNAPLTLLVPAALVFVIYVLARPTSWGSRAMQRSYD
                     QAPTLRAGLIALLITLTIGFLINDSGVAIPAVGATLAVPLILSASVTYLLDEARAAAQ
                     TRSARQRR"
BASE COUNT         4041 a        10258 c        10495 g         4233 t
ORIGIN      
        1 tagttggtca ggttgcggaa cccgagtgcg gagccgcgca ggtgttcgag ccgtccgttg
       61 agcgcctcgg tcggcccgtt gctggtgccg ggtcgttcga agtaggcgag cacgtcagcg
      121 gctcgcttct tcagggtccg gcccagggtg gtgagctcgg tgagcacctt ggggacgccg
      181 gcgctgaggt cggtgatcag cttctccatg agctcgcggc cacgttgccg gtcctcgtgg
      241 cgataggcgg cgatcatgcg ctggtagaca ccccaggtcg cctcgacctc gacgtgagcg
      301 tcatcaacga acagcgcgcg tagcctgtcg ctctgcttgt cggtgagcag gtccgcgccg
      361 gtgtgcagcg tgcgccgcga cttgtagagc gggtcgtccc tgaacccacg gtgcccgtgg
      421 atcgcgagtt ggacccggcg ccggcacctg tcgagggcgt caccggccag gcgcacgacg
      481 tggaagggat ccatcaccgt gaccgcgtcc gggatctcct ctgcagcggc ggtcttgaac
      541 ccggtgaagc cgtccatcgc gaccacctcg accgcgtcac ggaaggcgtc gtcgcggtcg
      601 gcgagccagg tcttgaacgc cgccttcgac cggccctcga ccatgtccag cagccttgct
      661 gggccggcgc catcgcggac cggggtgagg tcgatgatca cggtggcgta cttgtcgcca
      721 cgcctggtgt ggcgccagac gtgctcatcg acgccaatga ccttcacgcc ctcaaaccgc
      781 gtggggtcgt tgatcagcag ccgcttgcct tcagccagga ccgcgttgtt ggcggtgtcc
      841 cacgcgaccc cgagtccctc ggcgacacgg gcgacggtga ggtgtgcgac cacgatccct
      901 tccagcgccc accgcagccc ggtgcgcgag agcttcgcgc gtggctccgc cgcggcgctg
      961 gtgtcttggc gccacacgtg tccgcagtcg gcacagcggt agcggcgcac tacaacttcc
     1021 agcacggtcg gtcgccagcc cagcggctcg tgggccaacc gccggatcac ggtgtcacga
     1081 gcagcgcctt cgctgccgca ccgtcggcac cacggatctg gttccaccac gcggcacgcg
     1141 aggaccgcac gatccggttc aagtcgttgt ccggtcacgc tcagaccgag gccgtcgagt
     1201 cgagcgaagg cggtcaggtc agggcggccg aagccggccg gcggggtagc gtcggacacg
     1261 tcgaggtctt tcggatggat ggcgtaggaa cctccgtcgt cgggagacct cgacgtctat
     1321 ctgcggaccg acgcgcccgg ccgacctgca ccgtcatctg agaagacccc tgaaacgtct
     1381 tcgacgacga cgggaccggc gaggaggagg gtgcggtgag ctaaaggaat ggagcctgct
     1441 cggcttcgac gccgccgcaa gctccggtga aacggtcggt actggtgggt cgtcgtcggt
     1501 caccaccgga tggcggcgtc ccccacgctc ggcgtttgcg caggtcagcg gctcgttcgc
     1561 ccagtgggcg catcaagcga tagacgcaaa gccgacgtga ttaggtcgtc ggttcgattc
     1621 cgacaggcgg ctccggcgaa cagcccctga cctgcggaaa cgcagggcag gggcggttcg
     1681 gttctggtgg tcgcgcgatg cgctggcctc acggctctgc ccgacaccga cgtcgctctc
     1741 ctctgcgacg gacccctcgc cggctgagcg ctcgcggccg gcatcctgtc cgtgtgccga
     1801 taggcctcgc accgctcggc aacgcagcgt ggcgttgcgt cgcgacctgg cgacggctcg
     1861 gcggggcgcg atcgtctcgt gcgttggcgg actacgggag cttggacgcg aggacgtcgg
     1921 ccgccaggat ctcgggtgct gcgccgagga ggtgctggtt ggccatcagg gctgcgacga
     1981 tggcgccgtt ggcgtgggcg tcgcgagcgg cctcgtcggg gaatgcatcg aagatccaga
     2041 aggtgtcggc gtgggtcctg accgcgaacc agacaatcgt tcctacttct tcgttggcga
     2101 gcgcgacagc gccggcgagc agatcggcga gcgcgtcgtg ctgtccatcg gccgcgacga
     2161 tcttggcgac gaaggcatac ggaagtgatg cgggtgtgga catgaggggt tctcctaggt
     2221 gtcgaggttt cttggcgaag cgagttcagc gtatgacgag caagggcatg tgagaagtgg
     2281 cgtatacggc agtattgcta ttgttcgcgc catgcgtatc ggactgatcg cgatcgacgg
     2341 ctgcttcggt tcggctgtcg cgtcggtcat cgacatcgtg cgggtggccg acggagcccg
     2401 cggcgatgtc gacccgcgga tcgacccgat cgaactcgcc atcctcggac cgaaacggag
     2461 agtgaccacg acggcatcga tgaccctgtc ggtggaccac ccgctgtcgg agtccgcaga
     2521 gttcgacgtg gtcgtcgtcc ctgcgcttgg aaccctcacg gccgccgcta ccaacgacgc
     2581 cctccagagc cgagatgctc gttcggtcat cgcctcgctc gggcgcctcg acgacgcgac
     2641 cacccggatc gccgcggcgt gcaccggcgt gttcgctgtc gccgagaccg gacggatgca
     2701 tcatcggcgg gcgacgacca gctggttcct ggggccggag ttcctgaagc gctatccgac
     2761 cgtcgccctc gatctcgaca ccatggtcgt ggtcgacggg aacctcgtca ccgccggcgc
     2821 cgcgttcgcc cacatcgacc tcgcgctctc actcgtgcga tcgatcagcc ccgacctggc
     2881 ccaacatgtc gccaagctcc tcatcatcga cgagcgtccg tcgcaggcgg ccttcgtcgc
     2941 ctacgaacat ctccggcacg aggacccgat cgtcgtcgag ttcgaacgct tcgtgcgcgc
     3001 ccgcctggac gaaccgttca acgtcgcctt cgtcgcgcag tcgctcggca ccagccggcg
     3061 caccctcgaa cgacgagtcc gtgcggcgct caacctcact ccgctcggct tcgtccaacg
     3121 gcttcgcatc gaacgagctc ggcacctctc agcaaccacg gaccttacct ccgccgagat
     3181 cgcgctacgg gtcggctacg cgaacgccga gactctgcgc tccctcctgc gtagggagcg
     3241 acgccgttcc tgacctatcg ccatgcctgt agccggtgtc ctagtgctcg actggaacca
     3301 cctctcagca cgtcgcgtcg acgctcctgc gtcgcccctg gacgacccac tcgacacgcc
     3361 cgcagcacag gccatgtgac gtgtcccggc ttcccggacg gtcggggttg ggtggctgtg
     3421 atgtcgctgt gttctcgtcg agggggtcag cagactcgat cagggtcgat tgggatgaga
     3481 cgcgaaggcg gtcaggtcag ggcggccgaa gccggccggc ggggtagcgt cggacacgtc
     3541 gaggtctttc ggatggatgg cgtaggaacc tccgtcgtcg ggagacctcg acgtctatct
     3601 gcggaccgac gcgcccggcc gacctacacc gtcatctggg aagagcccct tgacgttctt
     3661 catgtcgtag gtctcgacga tctccttcga cagggcgtcg acgacgatga tgacgacctt
     3721 cgtcggtcgc tcggccgctg tcggtggagc cgtggcaccg gcaggaccgg ctgcggtgga
     3781 cagcgcgacg gccgcagcag cggcaacggc agggacggtg agcgtcaagg aacgtcgcac
     3841 gtgcaacctc gccatcaagc ggacggaccc cgttgtccgt gagcggaacc tactcctgcg
     3901 cggctccggg cgcaccggtt acttggcggt agtcccggtg gtgttgtcga cgatcacgcc
     3961 gccggcgttc gatccgcccg gccagcacga gaagccgacc ggaatcgcct ggccccggct
     4021 gaccatctcg ttgagcgtgt cgaggcagcg cgacaccagg acgttgggct ggtcggagac
     4081 cgacttcgcg aggatcgagt tcgccgcggc ctcggccgcc gcggtcagct tgcgctgctc
     4141 ggcgatgcgg gtgttgccga tctcggtctg gtaccggttg atccggtcct gggtctcctg
     4201 gtcgaagttg atgaccggga tggtcaccga ctcgatgagc agccggtcac ccagcttgtc
     4261 gttcaggcgc gcacgcacct cggtcgcgat ctcgtcgcgg gtcttctccg agccggtgac
     4321 ggtgcccagc gggttgtagt cctgcagcgc ggcgttgagg gcggcagcga gctcgggctc
     4381 ctcgagcgcg gtgccgatcc gctcgaaggt ctggtggtcc cggaacagtc ggtcggccgc
     4441 ctcgggccgg atccgccagc gcacgttgtt ctgcacgtac gcgatcgact ggttccccag
     4501 ccggatctcg gtgcgccggt ccccgaggtt gtcgtcgatc tggaccgtgc cgtccatgag
     4561 ggtgacggtc gtccagggca acttggggtg gatgccgttg ttcatcgggc tgccgggggt
     4621 cccgaacgtg acggggatgc cgacctgctg cacggggacg acggtgaacg acatcgcggc
     4681 cgtgtaggcc acggtcacca cggcgaaccc caccgcggcg aacccggcca agcggtgcag
     4741 gacacggcgc ccgtccacgt ccacgctcgg tcggccacgc gatcgggacc gccgctcgtc
     4801 gtcggcaccc tcgtcgccct cggatgccgg gtcctcgccg tccgcttgct cgtcggcgcc
     4861 ctcgtcggat gccgctgcac ccgaacccgt ttcgttcgca cccgcctctg gtcgggtggc
     4921 gaaccaggtc gccagggatg cgcccgtcag ggcaaggagc agcacgatca tcacgagggt
     4981 tcccacgctt tcggacccta cacaggcgcg caccgggtcg cgcgaggacg acgacggcgc
     5041 tgcgactcag gtgccggcca cgcccgggcg cggctcagcc ggtctgtctg accgtgacct
     5101 ggccgtcctc gacgtgccag cgctggtcca ggcgcacggt gtcgagcatc cgccggtcgt
     5161 gggagaccag gagcagcgac ccgtcatagc tgtcgagcgc ctgctccagc tgttcgatcg
     5221 cgggcaggtc gaggtggttg gtcggctcgt cgagcacgag caggttcacc ccgcgagcct
     5281 gcagcagcgc catcgcggcc cgggtgcgtt cgccggggga gagcgccctc acgaggctcg
     5341 tcacctggtc ggccttgagc ccgaacttcg ccagcagcgt gcgcacgtcc gccggggtca
     5401 tctgcggcat ggccgcctcg aaggcatcgc ccagcggtcg gtcctcggcc aggcccgtcc
     5461 tggactggtc gatctccccg accgccaccg acgcacccag gctcgcgcgg ccggcatccg
     5521 gcgtgagatg gccgaggagc aggcgcagca gggtggtctt gccggcgccg ttcgggcccg
     5581 tgacgccgat ccggtcgccg gcgctgacct gcacggatgc cgggccgaac gtgaacgtgc
     5641 cgagcgtcac cgacgcgtcg gacagggtcg cgacgaccga gctcgaccgg ggcgcgctgc
     5701 cgatggtgaa ctgcagtgcc cactccttgc ggggctcgtc gacctcgtcg agccgggcga
     5761 tccgcgactc catctggcgc accttctgcg cctgcttctc cgatgactcc acggccgcgc
     5821 ggcgccggat cttgtcgttg tcgggcgcct tcttgatggc gttgcgcact ccctgcgagc
     5881 tccactcacg ctgggtgcgg gcgcgggcca cgaggtcggc cttcttgtcg gcgaactcct
     5941 cgtaggcctc acgcgcgtgc cgccgggcga cggcccgctc ggcgaggaac gcgtcgtacc
     6001 cgccgtcgta caccgcgacc tggtgctgcg cgaggtcgag ctcgacgacg cgcgtgacca
     6061 cccgtgacag gaactcgcgg tcgtgcgaca cgagcacgac tcccgaccgc agaccccgga
     6121 cgaagtgctc gagccgctcg agcccggcga ggtcgaggtc gttggtcggc tcgtcgagca
     6181 gcacgacgtc gaaccggctg agcaggaggg cggcgagggc ggcccgggcc gcctggcccc
     6241 cggagagcga ggtcatcagg gcgtcggccc cgacggtcag cccgagatcg gccaggaccg
     6301 gggagaggcg ctcgtcgagg tcggcggcgc cgctggcctg ccagtggtcg agcgccttgg
     6361 cgaaggcgtc ctcgctcgcg cgggtcacct cgtcggcgcc gagggctgcg gcggcagcct
     6421 ccatggatgc cgtggcggcg gccgcgcccg tgcggcgggc gacgtattca cccacggtct
     6481 cccccggcat ccgctcatgt tcttgaggca accatccgac gaaggcgtcg gtcggagcag
     6541 tcgacacaga cccctcgagc ggggccaggt cgccggcgag gatgcgcagc agggtggtct
     6601 tgcccgctcc gttggcgccg acgaccccga cgacgtcacc gggggcgacg gtgaagtcca
     6661 ggccctcgaa cagggttcgg tgtgcgtggc cccctgcgag gttcttcacg acgaggtcgg
     6721 cactcacccc acgatcgtag gctgcaaggg ctgataccac tttggtatca tgcagtcatg
     6781 gccatgacgt tgcgactgac ggatgaggac gagcgcgctc ttgccgcctt ggccgccgct
     6841 caagggatca gcaagcagga ggcgacggtg cgcgcgatcc gcgatgccgc agcgcgcggc
     6901 ttgcacgagg ccaaggtcag cgaactctcg gcgcaggcgc gcgagcgcta cgccgatgtc
     6961 ttggatcgcc tcgggcggtg acggtctacc tgtccttgga ggatctgatc gccctgacgc
     7021 gcgacctcga cgtgggcccg gttcgggatc tcggactctt ggatgcggcg gcccaccgcc
     7081 cacgaaccga gttgtacggg cagcaggctt atccggatct cgacaccaag gcggccgtcc
     7141 tgcttgattc catcgtgcga aaccatgccc tggtcgatgg caacaagcga ctcgggtggc
     7201 ttgccacggt cgtgttctac ggaatcaacg acgtcgacct cgacgcccct gacgacgacg
     7261 cctacgaact ggtcatggcg ctcgcccgaa gtgagtccac tgttgaggag ttggcggcgg
     7321 cgctccggtc gtggcactga ttgccacacc tcgacttcgt gaccgagctg ccgccacacc
     7381 tccccgactt cgtgcgactt gagtgcggat ccgggcccgg atcgggacga atgccgcacc
     7441 gaagtcgcac gaagtcgggg atgggggggt caggtgcggg tggtgccgcg gatgcggccg
     7501 ccgcctccga ggacgcgacc catcaggctg cccaggcggt tcgcggaccg tgggcggatc
     7561 cagccgatgc cgtcgtggcg caccttgggc tggtcgagca cgaggccgcg ccggtcagcc
     7621 cgcagcttga cgacgaagcg ctgcccaccg acgggctcgc cccggtcggc ctgcagctgc
     7681 cacacgtcga gcgcgacgat cccggcgccc aggcgctccc tgtgcgtcgg gacgaaacgc
     7741 agcccgaggg cacccagctg gccgggctcg agggcgaccg ggccgagtga ggccttgcgc
     7801 ccggcgatgc ggaagcgacc gtcgccgatc tcctccaacc cctgggactc accgccgctg
     7861 gccttccaca gctcgcgcag ctcgtcgctc ggctcgagcc agacctcgcc ccagtcgagc
     7921 atcgaccgct cgcgccgcgg ggcccggaac accacgtgca ccttcgtggc ctcgcgcccg
     7981 tagttggcga cgatcgcggc gctgacgcgc ccgtcggtgt cctcgtcgac gacggtgatg
     8041 ttcttccaga cgatgttgtt gttctgctgc acgttcgcgt agaggttgcc cgtctcgggt
     8101 gaggtcatcc cgaagtccgg ggccgggccg gtctcgatgc gggccagcag gcagaagtgc
     8161 ccgcggtcgg caccgaacga ggcgtagtcg gccgggtccg gcggggacca cgggaacgcg
     8221 aggatctcgt cgtcgcccgc ggacaccgac accgcggcgg agccgatctc cccgcccatg
     8281 agcgccggtg acgtgacgct gccgtcccac ggcgccggcc aggcgagacc ggtcgaggcc
     8341 ttggcccagt acagccggac cgtgcccgac gacgtgccgg agcacccgcg gttgcgcacc
     8401 cgcacgtaga ccgacgtggt gcccgacccg tgctccgggt tctggtggtc ctggttcgcg
     8461 atgccgttgt tgtcgttgcg cacccagatg tcgtcgctga cccacatcgg ctggcccgac
     8521 gggttgggct cggcgccgtc gtcgtcggag gtgtccttgg cccacaggtc gttgacggcg
     8581 ccgctgctcg gcaccagccc gtcggaggcg agcagcccgg ttcgggccgt gtgcagggtt
     8641 gcgtccatgc gctcgctctg ccccgcggtg aacatgaaca tgccggcgtc gtcggtgtag
     8701 tccatgtagt tcatgaacat gtcgccgtcc gggccgttgc tgcacgacac ctgcgggaac
     8761 gccggggtgc cgtagtgctc gtcggcctgg ttcggggtgt ccgcgacgtt gtcggtgccg
     8821 gtgcacaggc tgccgtcgtc gccccagatg tggttgaggt tcaggtaatg acccacctcg
     8881 tggaccatcg tgcggcccag gtcatagggc gcgctgaggg tgcccgtcga gccgaaggcc
     8941 cggtagtggc agcgcacgcc ctggttcggc atgccgggga agctgccctt gccgaggagc
     9001 ccgtcggtgt agttgcacac ccagacgttg aggtacctgg tgacgtccca cgggtcgtgt
     9061 cccccaccgg ccgcggacag catcccggtc tggttgcggg tccacccggt gaccgaggtc
     9121 tcggtgcggg tgatgccggt ggtgaccgcg caccccggtc cgcgcacggc cagggcgaac
     9181 tcgacccggg cgtcggcggc gaccccggcg aacgccgagg ggacgctccc ggcatccgcg
     9241 ttcagccgcc ggaagtcggc gttgagcacg tcgatctgcg agtggatctg ggcgtcgctg
     9301 atgttctcgg cggcggtgtg ccagatgacg tgcacgacga cggggatgcg gatgatcccg
     9361 gtccgcgacc cctcctcacc gaaggcggcg atccactgct ggacgtcctt ctcgatctgc
     9421 tcgcggcgcc agcggtactc ggggtcgagg cgcacgagct ggcggtgctc ctccatcgtc
     9481 ccgcagcggt ttcggcgccg acccttgccc gggtcctgcg ggtcgaggtc ggtgggcact
     9541 gcggggaagc gggggtcgag ggggatgcgc gggttgcgac ggatgggatc ggcgaaggac
     9601 atggcgtgct cctggggtgg gtggtcatgc gtggctgttc acccaagagg agcgccgcgc
     9661 cgcccagcct gataccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgag
     9781 gatgccgggg ggcaccgtgg gtgcccacga tgcggtcgcc gtgggacggc atccgtcgat
     9841 catgaataac atgagataac cgttctgcaa acgaggagtt atccaatgag catccgtctc
     9901 ggcgacatcg cccctgactt caccgccgac acgacgcagg gggtgctgtc cttccacgac
     9961 tggaaggagg gcgcctgggc cgtgctgttc agccacccgg ccgacttcac cccggtctgc
    10021 accaccgagc tcggccgcac cgccgccctg tccggtgagt tcgccgcacg caacgccaag
    10081 gcgatcgcgg tgtcggtcga ctccgtcgag gaccacctgc gctgggtccc ggacatcgcc
    10141 gaggtcgccg gggtcgacgt cgacttcccg atcatcgccg acgaggaccg ctccgtctcg
    10201 caggcctacg acatgatcca cccgggcgag ggtgacacct cgaccgtgcg ttcggtcttc
    10261 ctcatcgacc cgagcaacac ggtgcgcctg acgatcacct acccgaagtc ggtgggccgc
    10321 aacttcaccg agatcctgcg cgcgctcgac gccctgcagg ccaccgaccg ttcgccgatc
    10381 tccaccccgg ccgagtgggt gccgggggac cgggtcatcg tggcgccggc catcgccacc
    10441 gacgacgcgc gcgagcgctt cgccaacgtc gacgaggtca agccctacct gcgcttcgcc
    10501 gacgccccgg cctgacgcca gacctggtcc gcccggcgcc ctcagggagg ggtcgggcgg
    10561 accagggccg cgtagtcggg atgccgggcg atccacgcct tgatgaacgg gcaccgcggc
    10621 acgaccctca gggagtcgtc ctgtgcgacc tcgtcgaggg cccccctggc gagcgcccct
    10681 ccgacgccgc ggccctcgaa cgccgggtcg acctcggtgt gggtgaacac gacgtcgtcg
    10741 tcgaccacct ggtaggccgc gaaaccggcg aggacgccgc cgacgtgcgc ctcccaccgg
    10801 ctctcgtcct cgttgcggga caccacctgc tcggccgggt cagccacgcg agcacctccg
    10861 ttcgttgacg acgacacgtg tcgaggtgca ccactgtggc gcatcacggc gcccaccggg
    10921 gcctgcaccg cccctcggcc ctacgctggc acggtgaacc ccgcgatcgt cgagaaggcc
    10981 gcccgtcgcg tgggtcgcag cccgttcctg ccggccatcg gggacgccga ccgcgtcttc
    11041 gagcgggtgc tctccggagt cggactcatg gtggtgccgt gggcgttcgc ggcatcgctc
    11101 gtgccgtcgc tgctcccgcg cgccccgtgg ctgcagggtg tcgcctcggg agtcaccgtt
    11161 gccgtggggt acgggctggg ggcggcggcc cacgccctct ggcgctacct gcagatcccc
    11221 ggcctcaagg gccgagcacg ctcgatcgcg ctgggcctgg tcatcggcat cggcatggtc
    11281 ctcgtgctgc tgagcgcctg gaagttcgtc ggctggcaga acgacatccg caggatcatg
    11341 cggatggagt cgacgtcacc cacgatctgg cccgtcgtca tcgtcgtgac gctcctggtg
    11401 ttcaccctgt tcgtcctgct cgcccgcagc ctgatcgtgc tggcccgcac ggtggcctcg
    11461 tggctcgggc gccgactgtc gcgccgtctg tccatcacgc tcggcgtggc ggcagtgctc
    11521 ttcctgctct gggggctgtg gaccggggtc atcgtcaacg gcttcttcgc cggggccaac
    11581 gccttcttcg ccccgcagga caccaccggc accaacagct ccaaccgccc cacggcgcct
    11641 gaacgctccg gctcgccgga gtcgctgtcg ccctgggagg acctcggcca gtacggccgc
    11701 ttcttcgtgc gcggcggccc cgacgtcgcc gagctcgaag ccgtcaacgg tcccggcgcg
    11761 aaggagccca tccgggtgtt cgccggcctc cagagtgccg acacggtcca agggcgagcg
    11821 gacctcgtgc tcgaggagct caagcgcacc ggggccttcg accgcgaggt cctcgtcgtc
    11881 gccacgacca ccgggatggg ctacctcgac caccgcggga ccgaccccct cgagtacctg
    11941 tggaacggcg acaccgccat cgccggggtg cagtactcct acctgccgag ctggatctcg
    12001 atgctcgccg accaggatgc cgtcgcgagc acgtcgcggg tcgtcttcga gaccgtgcac
    12061 cagtactggt ccacgctgcc gtccaacgac cgccccgacc tctacctgta cggcctctcg
    12121 ctcgggtcgc gcggcgtcga gagcgtgctg tcgtcgatca gcatcgtcaa cgcgcccgtg
    12181 aacggcgccc tcatgtcagg cccgccgttc gtgaacccgc tgcacgcacg cctccaggcc
    12241 gagcgcgatc ccgactcccc ggcatccctg ccgatctacg aggacggccg cacggtgcgg
    12301 ttcgcgaact ccgagggcgg gctggagcgc ggcggggctc ggtggggccc cacgcggctc
    12361 gtgtacctcc agtacgcatc cgaccccacg gtcttctact cgccgaacct cgcgttccgc
    12421 gaacccgact ggctcaagga gggccagcgc gggccggacg tcgccccgac gatggagtgg
    12481 ttcccgttcg tcacgatgtg gcaggtgctg ctcgacatgc cggcgggcac ggcggtgcct
    12541 gccgggttcg gtcacatgta ctcctcgcag gacaacctcg actcgtgggc cgcgctgacc
    12601 caaccccccg gctggggacc gggcatgcgc gaacggctgt cgaccgcgct cccgagcgac
    12661 tgacgggtga gcgcggctaa ggtgggctga ccacccgcac gagccaggag aaccaccatg
    12721 tcggcaccag cgggttggca ccttcagccg gacggccggg aacgcttctg ggacggcacc
    12781 cagtggaccg accagttccg ctcgccggcc ccgagcgacc cgaccgcgcc cccacccgcg
    12841 ccgtcgtggg cgagctcgtc ggacaccgtc gctgacaccg tctcggacag cggccacacc
    12901 caggccttcg acgtggccgg tacgcaggcg cttccgcgtc aggatgccgg gacaccgggt
    12961 gcgtacccga ccgccggtca ggcgccggct gggtacccgc agcaggggta cgcgccgggc
    13021 cagccccaga ccgggtacgg cgcaccgggt gctgccggtt accccccgcc ggtcaagagc
    13081 ggcaacagcg gtctggccaa gggctgcctc atcgccgccg tcgtcatcct cggcctcatc
    13141 gccatcgtca tcgccgtctt cgcgttcttc gcctcccgcg tggtcgacac tgtcaacgag
    13201 accttcccgt ccggcttccc caccagcctg ccgagcgggt tcccgaccga gctcccgacg
    13261 gacctcccaa gtggcctgcc gaccgacctc ccgagcggtc tgccgaccga catcgagcag
    13321 aacatcgagc tcgacgtcgg cgacggcttc gagctgccgc gcgccacgat cggcggcggg
    13381 tggacgctgg tgccgcaggg tgccggcatc cccatcgtca acatcaccgg catgaaggcc
    13441 accctgggcg acgccaacgg gttccccgtg ctgttcacga tgcgcttccc cgccgctggc
    13501 ggtgaatcgg tcgagacggt ctgcaccgcc ccggccggtg agccggatgc cgtcgtggac
    13561 gtctcgtgcg tcccgctgtt cggtgacgtg gccgaggcca cgacggtgac cgtcaccgcc
    13621 tccctctgag cggtccgcgg acttcacctg gcaaacccga ggttcgcggg ggatgacaac
    13681 ccttgcgaac gtcgggtttg cctcatgggg agggaaagct gaggtcagcc ctcggcgggc
    13741 ttgatggcga cgagctcggc gacgagctcg tcggtgcgca gctggtcggc ctcgagctcg
    13801 ggggtgaacc cgagccgcac gacgacgagc tcggccgagg gcacgacgta caccctctgg
    13861 ccgtcgtggc cgctcgccca gtaggtgtcc tcgggcagct gggggtcgac gagcgacccg
    13921 tcggccttct tgttggccca ccacccggcg gcgtagccct gctcctcgct ctggcgcacc
    13981 tcggtcaccg tggtggaggc cttcatccag ccctcgggca gcagccgctc gccagcccac
    14041 acgccgtcct ggagggcgaa ctgcccgatg gtggcccagt cacgcggcgt cgcccacagg
    14101 tacgaactgc acacgggggt gccggtcatg tcgacctcca gcgtggccga cgtgagcccc
    14161 agcggggcga acagctgctg gcgtggcagg tcggcgtccg gggcgtcggc caggtcggtg
    14221 agcactgcgc acaggatgtt cgtgccgccg ctgctgtact gctggtacgt gccgggctgg
    14281 tgggccagcg gctggctcgc ggcgaacccg gccatgtcgg gctcggagta gagcatctgg
    14341 gtgatcgggg tgccgagcga gtacgtctcg tcccacgaca ggccgctcgt tccccgcatc
    14401 agctggtcga cggtgatgcc cttgcgctgg tcggtccact cgggcagcaa cgagtcgtcg
    14461 gtgatcgcga tccgttcctg gtgcaccatc cgcccgacga gcaggttcgc gacgctcttg
    14521 gtcatcgacc agccgagctg gggggtctca cgggtgaatc cttcggcgta acgctctccg
    14581 atgagctgcc cacccttgag cacgacgatg ccgcgggttc cgagctcggc cgcacggacg
    14641 gtgccgaggc cgtcgccgaa ggcgcgagcg atggcggcat cgacgtcagg gttcggttcc
    14701 ggggactccg ccgtggagag ggggttggtg tcgtggtcga ccttcgtggc ctccccgagg
    14761 tcctcgggcc ggtcggcacc caccgtgcag ccgaagcccc gggtgtacca ggccttctgc
    14821 gtggacagcg tgaacaggat ctgcgacgtg ctcgagcggc cggcctcgtt gacgtacccg
    14881 gtgaggaagg gcaccagggg gttgtcgggc aggtcggtct cggggtcgtc acggccggcg
    14941 acgagcgtca cggcgcagga gttgtgcgcc gcgtaccccg ttcccgtctc gagcaggggg
    15001 cgggcgtaga agtacccgcc cacggcagcg gcgatgacga ggacgaggag agccgcgacg
    15061 atgcggaggg ccaggcgcat gggccggacg ctaccgcgtg acggcacccg ggcacacgcc
    15121 gcccgggcct gctccgtgca gatgccggcg tcggcgggac gtcccagatg ccgtcgccca
    15181 ggagcacgcg tccgccgggc gcgaggtgac ggcgcacggc atccaacgtc tcgcccggcc
    15241 ctccgaagac gtggctgacg ccgacggcga gcacggcatc gaacagaccg ccggtccacg
    15301 cggcggcatc ggcctccacg aaaactgcgc gcgaggcctg ccccggggcg agtgcgccgg
    15361 catccgaccc cacgtggagg tcgaccccga cggcagtgac accgtgtcgc acgctcagcg
    15421 cccggcggat ccactcaccc tgaccgcagc cgaggtcgag cagccgcccg ctcgacggga
    15481 gcaggcgatc gagcagtcgg gccaccgagg catccgacag cggtgccatc acggggtggt
    15541 gggcgtgggc gtggtggctg atctcgtggt gctgcacgtg gctcactgtc aaggaccgcg
    15601 atgcgtgcgc gcaaccgata tccgggcaca tcgcgttacc gtgagcaccg caacggtggc
    15661 tgcggccccg actcatgacc cgaaggaaga accagtggct ggtggactcg tcgccctcct
    15721 cgacgacatc gcggcgctcg cgaagctggc ggccgcctcg gtcgacgacg tcggtgccgc
    15781 cgcagggcgc gccagcgcga aggcggccgg ggtcgtcatc gacgacaccg ccgtcacccc
    15841 gcgctacgtc gacggcctga agcccgagcg ggaactgccg atcatccgca agatcgccat
    15901 gggctccctg cgcaacaagc tgctcatcat cctcccggcg atcctgctgc tcagccagtt
    15961 cctgccgtgg tccctcaccc cgatcctcat ggtcggcggc acctacctgg cctacgaggg
    16021 tgccgagaag atctgggaga tggtcgccgg tcacgagcac gcggtcgagg ccgttgcggc
    16081 cggtgacccc gaccacgaga agacggtggt cagcggggcg gtgcgcaccg acttcatcct
    16141 gtccgccgag atcatggtca tcgcgctcaa cgaggtcgcc gacgaggcgt tcgtctcccg
    16201 tgcggtgatc ctggcgttgg tggcggtggg catcaccatc ctcgtgtacg gcgtcgtcgg
    16261 tctcatcgtg aagatggacg acatcgggtt gcacctggcg gggcgtgagt cgggagcctc
    16321 gcagcgcgtc gggcgtggcc tcgtcgcgtt catgcccaag ctgctcgccg gcctgtcgac
    16381 ggtcggcatc gtcgccatgc tgtgggtcgg tggccacatc ctgctcgtcg gggtgcacga
    16441 cctcggctgg cactggctct acgacctcgt ccaccacgcc gaggaggccg tcggtggcgg
    16501 cttcccgggc tggctggtca acaccctggc ctcggcggtc atcggtctcg ccgtcggtgc
    16561 gctcgtcgtc ctggtcgtga cccaggtcaa gaggatgcgc tcacccgagg gcgaccacgc
    16621 ggcatccgct cactgagcgc ttcgctgctg gcgccgggtg gcccgccacc ccaggaagag
    16681 tgagcccgcg aacagcgcca cggccatgac cccggcgagc acgaaggtcg tcgcgtcacg
    16741 acccgcggag gcgccgaggg cggcataccc gacggtgccc gggaggatgc cgatggcggt
    16801 ccccagcacg aagtcgcggg aggtcacggc ggtgatgccc gcgccgtagt tgacgatggt
    16861 gaaggggatg accggcgtga cgcgggcgat gatcaccgcg agcaggcctt gctggcgcat
    16921 gatctcctcg acccggtcga tccggggccc ggtcatggac gccacggctg tgcgccccag
    16981 ccggcgggcg atggcgaagg tgacggcggc actgatcatc gacgccaccc atgacagggc
    17041 gatgccaccc acgagtccga acagtgcgcc cgccacgcct gcgagcaggt tcttcgagac
    17101 gggcacgagg gtgagcagca cgtgcagcgc gacgtacacg acgggacccc aagccccggt
    17161 ggactcgacg gtggactgga ccgtgtcgag gtcgccgccg atgacgaccc aggcgagagc
    17221 gctggcgatg gcgacgctga ggacgagtcc gagcaggcgc agcctggccg cccgggtgct
    17281 catcacccgg tcagcctagg cgtagggacc gactgcccag gacgatcagg gtcagcggcg
    17341 ggtgggccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gacgctggcc
    17461 atgaccatgt cggcgtgcat gccgcacccg gcccaggtcg tcttgccgca ggagtgacag
    17521 gtggcggggc tgcacatgaa ggacctcaca aaggggtaga ggagtggtgt ggaagatagc
    17581 gtaggatata cccctggggg tatgcaaggt gagccgaggc gaggcccgtg aggtcgctgt
    17641 tgcgggcagg aagtcccttc gctctcgctc gggcccgacg gcccgcctca gcgacccgag
    17701 ccgaggtgac gcgaccggat cgccaccgcc ccgagcaggg cgacgaccac cgcgacgagg
    17761 ccgaagccga gccccggccg cagggggatg gcgatgccga tggctccgcc gagtacccag
    17821 aacccctgga ggatggtctc ggaccaggag aacacccgcg cccgcacgtc gtcggcgatg
    17881 tcgcgctgga tcagggcgtc gaggcagagc ttcgcgagct gaccgaacag ccccgtcgcg
    17941 aggcccagca gcacgagcgc ccacaccgag tacaggatcg aggtggcgag ggcggcgctc
    18001 agggcgacga gggcgatcgc cgtcgccatc cgctcaggag gtggtgtccg cctgcggttg
    18061 ccgatgaccg accccaacgc gttccccgcc ccagcggcgc ccgcgacgag cgcgaggacg
    18121 accggcccgg accaccccgg gatcgggtgc tcgcgcatga ggaaggcgag gaagagggtg
    18181 aggaaccccg acaggacgcg ggctccgcag gtcacccaga gcgtgaagcg caccagcggc
    18241 ggcagcgcac gcaggcgtcg agatccgccc tcgagggtcg gcagcggggc ctcggagacg
    18301 gggatgccgt cgacgtcgac ctcgcccgtg gtcgagtcga cccgcgcggg aagccggatg
    18361 gcgagcaccg tcgccgaaac gtagatggcg aaggcgaggc gcagcgacca gtcgggcccg
    18421 atgcgtgaca gggcggccgc gaggccgccg ccgagcgcca tgcccaccag tccggcgatg
    18481 ttgaggcggg cgttggccgt gacgagggtg atgccgtcgg gcagcagccg ggggacggcg
    18541 gcggcgcgtg ccacggcata cgaacgactg gccacgaggc acccgagggc cgcggggaag
    18601 agccacgcgc tgtcctcgac cacggccgag gcgagcaccc acgcgaggaa ggcccgggtg
    18661 gccagggtcg tgccgagcgc ccagcggcgg ccgtgccgga acctgtcgag caacggcccg
    18721 atgagggggg ccaggagcac gaacggagcc atcgtcagca cgaggaagag ggcgacccga
    18781 ctgcgcgctt cgtcggtggg cagggcgaac acggtgccgg ccagcgagac ggtgagcgcg
    18841 gcatccgcgg cgacgttgac cgcgtgcagc tcggtgaggc gcgcgagccc ggtgtccgcg
    18901 gctccgtcgg cgctcgcgaa ggagcggaac cggcggccgg ccgctcgccc cccggtgccg
    18961 acggcgcgga tgccggcccg gccccctcgc ccgaccgtgc gccccgcgtc ggtcacgcgc
    19021 tgtcgcatgc cggacgtgct gcgagcgccg gggcctgcgg ggttcggcgg agtcggggag
    19081 tggtcgttcg tggtcatgac cctccgatcc tctcccgcgg agccgtggtc gagctgccag
    19141 aaacgcggac gttcggccgc tgcccggcaa ttaccggcca cgcggcgaac tgctgcgtgg
    19201 cgatcgcagt gctgcgtcgc catcgaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnncga actgctgcgt ggcgatcgca gtgctgcgtc gccatcgaag tgctgcgtcc
    19381 gcgcgtgctg ggcggctccg gaatggccac cggcggcgag gcgttgagca gcccgtgaac
    19441 acccccatgt cgatggcaga gcgctacacg caggcccgtg gcctgttcga gcacggcgag
    19501 taccaggccg ccgccgactc cctgcgtgcc ctcgtggacg agagcgccct cgagccaccg
    19561 ctgcacggca ccaccgagct gcgggtgctg ctcggccggg cctacttcca ctccgcacag
    19621 ctcggccgcg ccgagacggt gctgcggacg gtgctcatcg agtacccgga cgacgcctac
    19681 gccaacctcg tgctcggccg caccctccag cgcctcggcc gggccgctga tgcccgaccg
    19741 cacctcgcga tggccgagct gctcggtggc tacgccggca cgggccggct cccggcagac
    19801 acgtcagacc cgacggactc atcggcgtga gcgactacgg gcacgacctc gagttcggtc
    19861 tcttccccac cccggatgcc gcgcgccccc accgcgtgct ggagctggcg accctggccg
    19921 aggtcagcgg tctcgacctc gtgagcgtgc aggaccaccc gtaccaggcg cgccacctcg
    19981 acgcgtggac cctgctctcg gtcatcgctg cccgcacgtc gacggtgcgg gtcgccctca
    20041 acgtcgcgaa cctgccgctg cgcaaccccg tggtgctggc ccgatcggcc gcctcgctcg
    20101 acctgctcac cggggggcgg gtcgagctgg gcctgggcac cggtgccttc tgggacgcca
    20161 tcgaggcggc tggtggcacc cggctgaccc ccggcgaggc cgtgaccgcc ctcgaggagg
    20221 cgattgccat cacccgggcg ctgtgggcgg gggagggtag cgtgcgggtc gacggtgagc
    20281 accaccgagc ccgtggcctg cactcgggcc ccgcgccggc gcacccgatg ggcatctggg
    20341 tgggggccta caagccgcgg atgctgcgcc tcaccggtcg cctcgccgac ggctggctgc
    20401 ccagccaggg ctacgccgcc cccgagacgc tggggccgat gaacgcccgc atcgacgagg
    20461 cggccctggc cgcgggtcgt tcacccgagg ccgtgcgccg gctctacaac gtctcgggca
    20521 ccttcggctc gggggccggc ttcctccggg gccagccgcg tacttgggcc gagcagctcg
    20581 ccgagctcac cctcgagcag ggcatggcga cgtacatcct cgcgacggac gaccccgacg
    20641 acgtgcgccg cttcggtgac gaggtggcac ctgccgtgcg cgagctcgtc gcggcggaac
    20701 gtgcccggcg ggccacgcag ccctcggagg ccgtggcgcg ggatgccgtg gcgacctcgc
    20761 cctcgcgggt ggaggcctcg gcgtcgagtg tcgagccgtc cgcgggtgcg ccccgggtca
    20821 gctccggggc accggccggg gggcgtgaga accggcccgt cgtcacgccg acgcccgacg
    20881 acggccggcg gctcaccggc atcctgccgt gggacgaggc gagccggccg acctacccgc
    20941 gccgccccga cgcgcaggag caggctgggt acggcccggc ccagctcgcc gtgccccagc
    21001 acctcatcga cgtgcacaac cacctgaggt ccgagctcac ccagctgcgc gacgtggtcg
    21061 accaggtcgt gcgcgggcac ctccaggtgg gggcggcccg gtcagcggtc aacgccatga
    21121 cgatgcgcca gaacaactgg acgctcgggg cgtactgcga gtcgtactgc cgcatcgtca
    21181 ccggccacca ctcgctcgag gacgccgcga tcttcccgca cctgcgagcg caggaccccg
    21241 acgcccgccc ggtgatcgac cggctcgagg acgagcacga ggtcatccac gacgtgctcg
    21301 acgacgtcga ccgggcgctc gtggcgctgg tcgccgacga accgggggcc ctggaccgcc
    21361 tgcggcacgt cgtcgacctg ctgaccgaca ccctgctctc ccacctggcc tacgaggagc
    21421 gcgagctgct gcacccgctg gcccggcacg gcatgacccg ctgacgttca ggcgggaggg
    21481 cggacgtgct cggcctccag ggccagcagg tgctccttcg ccgcggcgcc ccccgcgtag
    21541 ccgccgaccc cgccggatgc cggcagcacc cggtggcagg gcagcacgat gcacagcggg
    21601 ttgccgccga gcgccgtccc caccgcgcgc gcagcgccgg ggtgctccac ggctgacgcc
    21661 agctcgccgt acgtcgtgcg ctcgccgtag ccgacccgcg ccgcgagccc ggtgagcacc
    21721 tcgcgctgga agggcgtcgc gagcgcgagg tcggtcggca cgtcgaccga acgggcaccc
    21781 ccggcgaggt acgccgcgag agcccggcga acggcatccg tcggggaggg gtgacgcagc
    21841 acccgcgggc tcacggttcc cgcgatgcgc gtcaaccacc ggtcctcgga tgccgtgtcc
    21901 ggggagtacg cgcacgtcac caccgcgccg gtggagcgca cggcgatgag cagtcgcccg
    21961 acggcggtgt cctcgagcac gtaggagacg tcggtgggct cgagcacggg cggaccggcc
    22021 agcggcgggt cgaaggtggt gaaggggttg gtcatggctg gtcccggagg tcgaggcgga
    22081 gggtggcgat ggcgtcgctg acgaggcgcc ggctcatgcc cggcgaggtg ccgagcgccg
    22141 ccgccaccgc gcggtggtcg aggtcgccga cgaagcgcag cacgacggca tcccgctggc
    22201 gctgcggcaa cgccgcgacc cgggcccaca ggtcgccgtc gggcacctcg ggcgggagat
    22261 gggccggggc ctggtcgagc agtgacaggt cgtcgtgggg ggtggtgcgg cgggcggtgc
    22321 ggtggtggtc catcgcgcag cggtgcgtga tcgtgagcag ccacgaccgc aggttgcgcg
    22381 catgggtgag ccgcggatag gcggccagcg cctgcgtcca ggcctgctgg gcgacgtcgt
    22441 caccgtgccc gggcgcgagg ccgtgcgcga ggcgggcgac gtcgcgccag tgctcgtcga
    22501 cgaggtgctg gaagggggga aggctcacga tggtgcaacg gtaggccgcc cggatgtgtg
    22561 agcacggccc ttgcgtccgg cagattggtc gtcggggcga gcaagatgcc ggacgtagcg
    22621 caccctcacc ggagcgtccg gcgggtcgga tgccgagagc ggccacgttc gcgcgccgga
    22681 tgtggaaccg tggccctgtg gaggtcgtgt cagaggggct gagcaagccc gaaccgcggc
    22741 ggggcggccc gctggtcatc gcgcaccgag gggcgagcga cgaggagccc gaacacactc
    22801 tggcggcgta ccgcagggcg ttcgcccacg gcgccgacgg ggtcgagtgc gacgtgcggc
    22861 tgacggctga ccaccacctc gtctgtgtgc acgaccgccg gatcgagcgc acctccaacg
    22921 ggcgcggagt ggtgtctgct ctggaactgg cccttctcga ggaactcgac tgggcctcgt
    22981 ggaggaccgc gcaccgcccc ggcgacaccg agctgcccga ggccgacccc gagcgcggcc
    23041 acctgctcac cctgcggcgc ctgctcgaca ccctcgtcga caccggtcag gaggtcgtca
    23101 ccctcatcga gaccaagcac cccacgcgcc acggcagcga cgtcgagaag gcgctggtgc
    23161 acgtcctgcg ggccttcgac ctggctggcg gtgacctgcc ggggcgcccg cacgtgcgga
    23221 tgatgtcgtt ctcccacacc gcccttcagc gtgcccaccg gctggcgccg aagctgccac
    23281 tggtctacct cgtcgacgcg acgcgtccgc ggatgacgtg ggacggcatc ctgcccgccg
    23341 gcatcggggc cgtgggcctg gacgtgcgga tgctgcgcaa cccgcgagcc gtgcgcgcgt
    23401 tccaacgccg tggtcaccag gtgtacgtgt ggaccgtcga caccgacgac gacatcacgc
    23461 ggtgcctgga cctcggggtc gacgtcatca tcacgaaccg gcctgcacac gttctcgatc
    23521 ggatcgctgc ctcgggttag gttggttcga tgacccctcc tccgccgcac tcgtcgcact
    23581 cgtccgctga tgacgacgac tccgcagagg tcggctcccg gcgcaccggc gagagcggtc
    23641 tcgtcgtcgt cgagtcctcc ggccccgggg tggacagcat catctgcacc gtccggccgc
    23701 cgcacgaccc gtcgtcggtc ggtcgcatcc ggcgcgagat cgtcgcggac ctccaggccc
    23761 gcgagatgcc cgacgagctc gtcgacgagg ccgagctcgt ggcgtccgaa ctggtgacga
    23821 acgcgctgcg acacgcccgg cccctgtccg acggcaccat ccgggtgcgc tggaagatcc
    23881 gcggcgaggt cgtcgaggtc gaggtgaccg acgccggcgg tgacaccgtg ccgcacccgg
    23941 cgccgcgcac cgtgtggctc tcgtcggggc gggggctgcg catcgtgcgg tcgatcgcgc
    24001 acgagtgggg cgtgaccgag gaccgcaccg gcaccgtcgt gtgggccacc ctcggcggcc
    24061 ccagccgccg ccgcgccaac tgaacgctcc cggcatccga aacgtccggg cagccgatgg
    24121 tggcggtgca cgaaaccggt gccatcaccc cgatttctcg tgctcgtgcc gcgggccgtt
    24181 agggtgacgc gcatgggcaa ggcatctcga cgtcagcgtg gaaccgagcg cggcaccgcg
    24241 cgctcccgtc ccgggccggt gcccttcgag cgccgcccct tcgagggcct ggccggtgag
    24301 accgactggg tcgcgatgcg cgagatcctc ccggcggcca cggccacggt gagcctgcgt
    24361 cccgagcacg tgccggacgg ggcgcccacc gaggtgaccg tcgcgaccgt gctgccgatg
    24421 gcctggcccg gcctgcaccg cgccgacggc accgtgttcg tcggcacgca gtcgggcaac
    24481 gccagcggcg acgcctcgcg cgacctcgcc gggcaggtgc tggccgcgct ggccgccgaa
    24541 ccgggcaacc ccgtcgtgac gacggcaccg gccaccgccg acagcccgcg ccttcaggac
    24601 ctgctcaccg acgacaccac gttcgaggtc accgtccacg agggcttcga cttctgggtc
    24661 gagggccagg agctggaagg ggatgccgcg gcgtccctcg agcgcgccaa cgagaccgtg
    24721 gtgcccacga cgaagatggc ggccctgccg tcggcctact gggtgcgcat cggcgaccgg
    24781 gcccacatcc gcctcgtgct ccccgacgac gaggacgccg ccaccgacgc cctcgcccgc
    24841 atgcacgcca gcggcgacga cgccctcggc ggcgacaccc gcctgctcgg ggccttccgc
    24901 gcctgcggcc tgctcgtccc cgtgtgggac ctcgaccccg agctcggtgc cgacgactac
    24961 gaggacgagc tcaccgcgtg gtccacccgc tacgccgcgg ccctggcctc gaccgagtcg
    25021 ctcagcgccg acgagcgccg cgcccgttcc ggcctgctca gccgtcaggt gaccctgcgc
    25081 tgatgagtgc gctcgccgcg caggtctccg ccgtcatccc ctgcaaggac gaggccgacc
    25141 gcatcgcggc cacggtgcgc gccgtggcgg cgctgccgca ggtggggcgg gtcgtggtcg
    25201 tcgacgacgg cagcaccgac gacacctccc gcgtcgctcg tgaggccggt gccgacgtcg
    25261 tgcgccacga gcgcaaccgg ggcaaggccg ccgcgctcga gacgggcgcc cggcgggtgc
    25321 gcgagctcga gcgcgcggat gccgcggccc cctggcgcag tgccctcctc ttcgtcgacg
    25381 gcgacctcca ggagacagcc gccaacctgg gggtgctcac gacggccgtc ctcgacggca
    25441 ccgccgacat gaccatcgcg acgctgccgg cccagatcac cgcggggggc ggccgggggc
    25501 tcgtggtcaa cctcgcgcga cgcggcatcc ggcacctcac cgggttcacc gcggtccaac
    25561 cgctgtcggg catgcgctgc gtcgggcccc gcgccgtcga cgccgcgacc ccgctcgccc
    25621 gcggctgggg tgtcgagacc gctctcacgg tcgacgtcct gcgcgccggc ctcaccgtgc
    25681 tcgaggtgcc ctgcgagctc cagcaccggg tcagcggctc ggactggcgc gggcagctgc
    25741 accgggccgg ccagtaccgc gacgtctggc tggccctggt cgcccgcggc tggcggccgt
    25801 ggcaccggga ctgacccgcg cccaccggct ctgcgtcggt ctggccgtcg cgctgctgct
    25861 gctcgtcggc gcctcccgcc ccaacgcggc cacgcccgac ctctccccga gcgggtgggg
    25921 cccgggcacc ctggtgccga tcacgctggg accgggcgcg gtcactgggg tgctgtggac
    25981 ggcatacctc gtcggtggtc tcgcggtgct gctcggcctg cgcgccgggg tgggggcgct
    26041 gcgcacgtgg gccctgcccg tggggttggg cgccctggcg ctcctcacgg caccgttcgg
    26101 ttcgggtgac cacctcaact acctggcgta cgggcggatc ctcgtcggcg gtggcaaccc
    26161 ctgggtggag tcaccgatcg actgggccgg tggcgccgac ccggtgacca gccgggtcga
    26221 ggccccgtgg accaccgagc cgagcgtcta cggcccgttc ggggcgctgc tgcacgggct
    26281 cgcggcgctg ctcgggggcg actcgctgcg ccagggggtc tgggtgtggc aggtgctcgt
    26341 cgtcggggcg tggctcgggg tgcgctgggt gctgcgccgc gtgctcgacc cctcggcgca
    26401 cggacgggtc gacgtgctgt ggacgctgaa cccgctggtg ttcggggtcg gcgtgctcgg
    26461 ggcgcacatc gacctcatcg ccgctgccct cgcggtcgtc gccgtggggg tggtggtggc
    26521 gcggccgggg gtggtcggcg ccgttctcgg cgggggtgtc gtggccttgg ccggctcgac
    26581 gaagttcacg tatgccgtcg tgggggccgg gatcgtcgcg gcctggtggc tggtcgggct
    26641 gcgtgggggc gggttggtgc gccgggtggt ggccctcggg ggtgggttcg tcctcgtggc
    26701 cggagtgctg cacgcctggg ccgggccgca cgtctacgac cagctgatgc gctcgcgcca
    26761 ggccgtgtcg ctggcgaccc cgtggcggcc actgctggag tggggccggg acgtgtgggg
    26821 caacgagcag acccgggtgg cgatcagcct cggggcggcc gtgctggcgg tgctgttggc
    26881 gtggtgcctg ctgcgcctga gccgcccggc tgcgcgtccc tggagagacc tgttggccga
    26941 tggagggacg gatgccgtgg ccccaccctc ggatgccgtg gcaccggtcg cgctctgggt
    27001 cacggcctgc ctgtcgttgg ccttctcgct ggccgcgccc tactcgctgc cctggtacga
    27061 cctgctcgtg tgggccgcgc tgccggcact gcttcccggc ctcgtcgacc tcgtggccct
    27121 ggttcggctg accgccctgt cgatcgccta cgtgccgggc cgggtgctgg gcatgacccc
    27181 gcaggtcgag gagctcacgc tcggggtccg tcgagaggtg gtgccgtggg tgggtgtggc
    27241 gctctgggtg ctcgtcatcg tcgctggcgt gcggagcggg tctgcgctgc ggcgcgggcc
    27301 tcgtcgagca ggtacgtgac cgatgccgag aggatgagcg gcaccgccag ggtggcgccg
    27361 accgcgggga tcgccacccc cgagtcgttg atgaggaagc cgatcgtcag ggtgatgagc
    27421 agggcgatca acccggcccg cagggtcggg gcctggtcgt aggagcgctg catggcccgc
    27481 gacccccagg acgtcggccg ggccagcacg tagatgacga agaccagcgc ggcaggcacg
    27541 agcagggtca gcggggcgtt gccgagcagg atgccgaggt tctgctcgcc cttgcggatg
    27601 acgacgtcca gggcgttgcc gtcgacgatc gactggatga accgccccag gtgactgcgt
    27661 gacgcggccg ggcgcagcca gtcggcgacg ccgatgagga agaacagcgc gaccaccgag
    27721 ccaccgatga gcagcacgcg cttccacgtc atcgcgatgc cgagcacggc gaacacgagg
    27781 tagatgatgc cggggatgag ggcaggcggc ccgccgccgt cggcccccca gaacggcatg
    27841 gcgttgatga cggtcgcgcc cccgccgatg gcggccacgg cgatggcagc ggccctgcgc
    27901 cggttctgga gcaccagcca cgacgacacc gccgtgcaca acaggatggt ggccgtcgcg
    27961 aagagggcga aggtcgtgtt gcccatgccg tagaaccggc caccgaccac gggctggagg
    28021 cccatgaggg atgacagctg gagcctcgcc cccgtcatga tgtcgaccgc gagcacgagc
    28081 atggtcaccg ccgacacgaa cgccatcggg cccagggcga aaccgcgcca cggccctcgc
    28141 agtgccgccg tggcgatgag cgcgacgaac accccgacca cggccacgac ggccaccatc
    28201 tcgatcggga agcgccacca cggcacgagg ttggcgagga aggtcgagac gggcacgctc
    28261 gcggccgcga ccgcgagcac ccgcacccgc gagagcacct tcacccggct cgcctcggtg
    28321 ccgagccgtc ccttccacac gagcaggacg atgaggtaga tgacgagctg gccgtacgcg
    28381 aagacggtga agaacggctc gacgaggctg tgcacgtcgt ggctcgcctc gtcgtagtcc
    28441 cgcagggtcg tcagccgctg ctgggcccgc tgctgggagt tgtcgggcgc ggacttgacg
    28501 gtcatcgggg cgccgttgac ggcatccggc accgggagcc ccaccgaggc cagcaccgtg
    28561 gccgtgatgt cggggatctg ggcgagcccc tcctgcttgg tcgaccccga caccagcacc
    28621 ccgggcccga agtgcgggcc ccgcgcgatg accagccgaa gacgctcgct cgtcccggca
    28681 tccgagaggg aggcgacgat gtactcggca ccgttggggc ccgcggcgat gacctcgccg
    28741 atgcgggcgt cgatggcggc gacctggtcg gcgcggctgc cgggggcgac ctcgccgggc
    28801 gcgacgtcgt cggggtcacg caccgagccc acgtcgacga gggtcacggg gcaggcgttg
    28861 aggtcctcga gcagggtgtc gggcgaccac gtcgagtact gctcgacggt gcccgcagcg
    28921 gtcgcgccac ccaccgcggc gagggggtcg accgcgcgca cgcaacgccc gcccgcggca
    28981 gcggtgtcgc cgagcagacc caggtcggcg acgaagcgcc ggtcagctgc cgccgagacg
    29041 tagtccgacc acgtgctgac ggcgccctcg gggctcacgg tgggcggcgg cgggcacggg
    29101 cggttggccg ggttgtcggc atcgccggtg cgctcggcgg ccgcccggct gcccgaggag
    29161 atgccgagcc agccgtcgac ggggcaggtg ttggagttga ccgaccgggc caccagcgcc
    29221 gcggaggcgc cgtcgcgcag caggacccac aggttcgggg tggtcgtctc gtcgacgtcg
    29281 gaccagctga tgccgcccgt gccgacgagg acgaccgcgc cggcggg
//
LOCUS       DAOSOC010000010        28979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1624_length_28979_cov_9.417473, whole genome shotgun sequence.
ACCESSION   DAOSOC010000010 DAOSOC010000000
VERSION     DAOSOC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 28979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28979
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1624_length_28979_cov_9.417473"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PL014_01725"
     CDS             <1..307
                     /locus_tag="PL014_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT55841.1"
                     /translation="LATAFTEGVQATLDEFDVPWSVSQLGARSEYRFASPAPRTGEEA
                     AIAHDDDLDAYMHLAMCNRGILMTPFHNMALMCPETTRADVDRHTATFREVVAALYA"
     gene            complement(385..1335)
                     /locus_tag="PL014_01730"
     CDS             complement(385..1335)
                     /locus_tag="PL014_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOT55842.1"
                     /translation="MPDPAADRAEITLRLLAAPTDAGQSGSVSAGRVLEWIDKAGYAA
                     AAGWSGGYAVTAYVGNVRFTRPVEVGELVEVSARVVYTGRTSMHITVDVASGQPGTRA
                     LEPATRCLMIFVSVDDKGSSTPVRELVPRTLGEELEQQWAMNRVALRREVEAAMAREQ
                     YTDAGSAPRVVLRFLAAPTDVNWGGKVHGGIVMRWIDEAAHVLATQWTGNAHNVAIFT
                     GGVRFYRPLRIGHVVEVEARLLHTGTTSMHIGVHVRSGDPTAGADGMELTTFCRTVFV
                     GIDEQRRALPCPTWTPVTDEDRALDAHAVELVAIRDRFGN"
     gene            complement(1340..3316)
                     /locus_tag="PL014_01735"
     CDS             complement(1340..3316)
                     /locus_tag="PL014_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOT55843.1"
                     /translation="MPGQSIFRSLEPLLEQVSKPIQYVGGELNSTVKPWDVGGEHTVR
                     WALMYPDAYEVGVPNQGVMILYEVLNERADALAERTYAVWPDLEALMREHQVPQFTVD
                     AHRPVGDFDLFGLSFSTELGYTNMLTALDLAGIPLHAADRDESHPIVVAGGHAAFNPE
                     TIADFIDAAVVGDGEEAVLAITDIVGAWKREGMPGGRQEILLRLARTGGVYVPQFYDV
                     SYLPDGRIQRIAPNPAYPQVPWRVAKHTVMDLDAWPYPKQPLVPLAESVHERMSVEIF
                     RGCTRGCRFCQAGMITRPVRERSITGIGEMVERGLAATGFEEVGLLSLSSADHSEIAS
                     VAKGLADRYEGTNTGLSLPSTRVDAFNIDLANELTRNGRRSGLTFAPEGGSERIRKVI
                     NKMVTEDDLIRTVAAAYGAGWRQVKLYFMCGLPTETDEDVLQIAELAKRVIDTGREVS
                     GRRDIRCTVSIGGFVPKPHTPFQWAAQLGADETDARLDKLRQAIRSDRRYGSAIGFRY
                     HDGKPGIVEGLLSRGDRRVGRVIEAVWRDGARFDGWSEHFSYDRWMAAAERELSAFGV
                     DVAWYTTREREEAEVLPWDHIDSGLDKEWLWQDWLDSLDETEVEDCRWTPCFDCGVCP
                     QMGTQIEIGPTGKSLLPLTVLGAGRAEFAEHSHA"
     gene            3394..4002
                     /locus_tag="PL014_01740"
     CDS             3394..4002
                     /locus_tag="PL014_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOT55844.1"
                     /translation="MSGERAVGNPLGPAAVRDAMAAQPRAGFLPRAVRQRAGFDGPLD
                     IGHGQTNSQPRTVAAMLELLDVRPGHRVLDVGSGSGWTTAILAQLVGPAGSVLGLELV
                     ADLVEQGSSNVARAGVPWARIRAADPSRLGAPDEAPFDRILVSAMADTMPRELEDQLA
                     ADGILVVPVAGWMLRVRRDAAAPGGVVVTSHGAYRFVPLVTH"
     gene            complement(4104..5978)
                     /locus_tag="PL014_01745"
     CDS             complement(4104..5978)
                     /locus_tag="PL014_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT55845.1"
                     /translation="MPTAAPQINTPADEPVDQAIFDNEAQSVGHLFRNRVAASPNGPA
                     FMYANVKPEGDEWVTDTWAEVDVVVRELGAGLIALGVEPEDRVAIASSTRYEWALADL
                     ATMVAGGATTTIYPTTMADDVAFILADSGTKVIFAENADQLDKLRGIRASTPAVTRVI
                     VFDAEVDDNDPWTLNLDQVRQLGREYLAGDPGAIDARIDGIQHDQLATIIYTSGTTGR
                     PKGVRLRHSAWTYEGAAVASTHILNEDDLQYLWLPLAHVFGKVLLTLPLQIGFPTAID
                     GRIDKIVDNLAVVKPTFMGAAPRIFEKAYGRISMMMEAEGGVKAKLFHLASDVGREVS
                     ELREQGKNPSGMLALKHSFTDKVIGTKIRDRFGGRVRFFISGSAALNNDVARWFDAMG
                     MQILEGYGMTETSAASFVARPRGYRYGTVGWPLPKTEVQIAADGEVLLRGPGNMEGYH
                     NNPDATAETIDSEGWLHTGDIGEVDERGFLRITDRKKDLFKTSNGKYVAPSIIESTFK
                     GLCPYASQLVVHGADRNFVSALVTLDPDAMAGWAAANGMEGKSYTEIVSSDACREMVQ
                     GYIDQLNAGLNRWETIKKFTILENDLTIEAGELTPSMKLKRKVVADKYKDRLDAHYTG
                     "
     gene            complement(6205..7167)
                     /locus_tag="PL014_01750"
     CDS             complement(6205..7167)
                     /locus_tag="PL014_01750"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOT55846.1"
                     /translation="MHRWSDPSETPEALRPCVVTLGNFDGVHRGHRAVLNALIESGHR
                     LGLPSVAVTFDPHPVAVLRPESAPELIAPGEIRDDLIEDTGIDGLLVMDFTQEFAKQQ
                     PEDFVRAVFVRALSARCVVVGMDTRFGYRNSGDVSTLADLGEKFGFEVVTLDDVGDGE
                     RFSSTVVRTHLRAGEVGEAARILGRPHRVVGTVVHGNHRGRGLGYPTANLSADSLGLV
                     PQEGVYAGWLTRLDRSPGEPDRTMPAAISVGTNPTFETQDRRTVEAYVLDRDDLDLYD
                     ERVMVEFTAHIRPTLRFDSVDELTEAMASDVARCRELLARIVPD"
     gene            complement(7177..8103)
                     /gene="truB"
                     /locus_tag="PL014_01755"
     CDS             complement(7177..8103)
                     /gene="truB"
                     /locus_tag="PL014_01755"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT55847.1"
                     /translation="MTTTADPTPDGILVVDKPAGWTSHDVVARGRRLCATRKVGHAGT
                     LDPMATGVLVLGVGRATRLLTFLVGCDKDYTATIRLGQATVTDDAEGEVTASAGASGI
                     EADAVRVAAATLTGDIEQVPSAVSAIKVKGERAYHRVRAGESVDLPARPVTVSRFEIG
                     DVRALEVDGTAVLDVDVEVTVSSGTYVRALARDLGAALGTGGHLTALRRTRVGGFTLD
                     DAHPLADLEARQGERMPVTPLAQAARAAFAVREVDEAQARTIGFGQRLPSEVPGREEV
                     VAAIAPDGRLVAMLDESRPTARSLVVFSPSTS"
     gene            complement(8087..8581)
                     /gene="rbfA"
                     /locus_tag="PL014_01760"
     CDS             complement(8087..8581)
                     /gene="rbfA"
                     /locus_tag="PL014_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT55848.1"
                     /translation="MADTARAQKIADRIKTLTAANLDAIVKDPDLGFVTITDVKVTGD
                     LQHASLFYTVFGDAAQRAQTARLLEQNRGKLRSFVGKQLGIRLTPSLEFIADAIPETA
                     AHLEDLLREAKERDAAVAAAAAGAVHAGDADPYKKDREVDEDDDVDGDDADDVDEAVG
                     DDDR"
     gene            complement(8702..11512)
                     /gene="infB"
                     /locus_tag="PL014_01765"
     CDS             complement(8702..11512)
                     /gene="infB"
                     /locus_tag="PL014_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT55849.1"
                     /translation="MSKVRVSALAKELGVTSKVLLERLNGMGEYVKSASSTVEAPVVR
                     RYNEKYPAEAPAAPAAPAAAPARPAPAAPSAPAAATPAPAAPAPAAPAPSAPAPAAPS
                     APAPAAPAAPAAATPAPAAPAPAAPSAPAPAPAAPAAPAPAAPAAPAAREGGSGQGAP
                     RPGAPRPNTPRPGNNPFAPSQGMGRTREPARPGNNPFAPSQGMPRPQSRPGGPRPGGA
                     GAGAAGPRPGGPRPSPGMMPDRSAVGRPGERPARGAGRPGAPGGAGARPGGGGGFAGR
                     PGGPGGFRSGPGGRGGTQGAFGRGGGRPGRARKSKRAKRMEFEEMQAPSIGGVSVPRG
                     DGTTIIQIRQGASLSDFADKINANPGSLVTVLFHLGEMATATQSLDEDTFKLLGSELG
                     YDIRMVSPEEEERELFDSFNIDLEIGVDSEDEEDLEARPPVVTVMGHVDHGKTRLLDA
                     IRNAEVAAGEAGGITQHIGAYQIHKEHEGVDRPITFIDTPGHEAFTAMRARGAKVTDI
                     SILVVAADDGVMPQTIEALNHAQAADVPIVVAVNKIDVEGANPSKIRQQLTEYNLVAE
                     EYGGETMFVDVSAKQGQNIDQLLEAVLLTADAALDLRANPDRDARGIAIEANLDKGRG
                     ATATVLIQSGTLHVGDAIVAGSSHGRVRAMLDEHGNALTEATPSRPVQVLGLSSVPRA
                     GDTFVVAPDDRTARQIAEKREAADRQASLAKARKRISLEDLNEALAAGKVDTLNLILK
                     GDVSGSVEALEDALLQIDVGEEVDLRIIDRGVGAITLNNINLAMASNAIIIGFNVRAE
                     GQNAEVAEREGVEIRYYSVIYQAIDEIEQALKGMLKPEFEEVELGTAQIREIFRSSKF
                     GNIAGSIVRSGEIKRGTRARITRNGVVVAENIEIAGLRRFKDDVTEVRDGFECGINLG
                     SFNDLQLDDLISTYEMREKPRA"
     gene            complement(11571..11939)
                     /locus_tag="PL014_01770"
     CDS             complement(11571..11939)
                     /locus_tag="PL014_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOT55850.1"
                     /translation="MATDRTGLRPGHPIRTCIGCRGTDSRSALLRVVLGTGADGIAVV
                     LPDPERRLPGRGAWLHPGISCLELAVRRRAFGRALRTSTPADHSAVEHWVRAHLQEPP
                     SEHETESGFDADEHPMSTQR"
     gene            complement(11951..13024)
                     /gene="nusA"
                     /locus_tag="PL014_01775"
     CDS             complement(11951..13024)
                     /gene="nusA"
                     /locus_tag="PL014_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT55851.1"
                     /translation="MDIDLTALRALERERGIAMDVLIQAIEQAITLAYQRTEGHYRVS
                     RAELDRKTGHVVIWAREELEVPAEEGAVDDEGNPVRPRRELGPEFDDTPSGFGRVAAA
                     TARQVIVQRLRDIEDDAILGDFKGREGELVAGVIQQSPVPRHVTVDFGTVEGILPLAE
                     QVPGEKYVHGQRLRCYVTSVKRGMRGPQIELSRTHPNLVRKLFALEVPEIADGSVTIE
                     ALAREAGHRTKLAVHTTVAGLNAKGACIGPMGARVRAVMAELHGEKIDIVDWSNDPAT
                     FIAAALSPSRVNSVTIVDPKLRAARVVVPDYQLSLAIGREGQNARLAAKLTGWRIDIR
                     PDTDPAAADAVAPSRADGASEGT"
     gene            complement(13026..13541)
                     /gene="rimP"
                     /locus_tag="PL014_01780"
     CDS             complement(13026..13541)
                     /gene="rimP"
                     /locus_tag="PL014_01780"
                     /inference="COORDINATES: protein motif:HMM:NF000930.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOT55852.1"
                     /translation="MTTADGIRPTVQDAVEGIGLVLEDVAVTPAGRRRVVKVLVDRDP
                     GPQDVADEPTDPLTLDEVADATRAVSDALDASDLMGEQPYTLEVSSPGVGRPLTLPRH
                     FRRNVGRLVTATHDGHEVTGRVLRASGTDVTLDIPATRSTPSRTETLAYAGLERAAVQ
                     VEFARPDDKDT"
     gene            13645..14721
                     /locus_tag="PL014_01785"
     CDS             13645..14721
                     /locus_tag="PL014_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55853.1"
                     /translation="MGEAARPGVSRRALLAASVGALALPSAACGIRLEDDAPRVPLVP
                     TRTPIPLESELVALTGGTLALAVLAGTVPGALAASLATIHRRQHTVLRTTLLRGQVPL
                     SLVDGPAASPSASSESPSGSPDPATSATGTPAPSASPTASTVESLASAEGESAVRAAE
                     FAGAEPDLLATLASLHAQRFAAATLLSGTAPTVAGAAVAGDAVEAMAASTSAAIYLFE
                     VVSARTSGRVRSRADTTLRVLRSVRADQVAGGASLGSTLGQPLPFPVTTTADAARLAR
                     EAMLALRSGYGEHLAALAATGATPGAAAVSGVDAGTAGDLAGGAAPGDAAGAGLAAAT
                     RWLGAVEVQANRWGVALAPFPGLT"
     gene            14740..15636
                     /locus_tag="PL014_01790"
     CDS             14740..15636
                     /locus_tag="PL014_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOT55854.1"
                     /translation="MSRVVLPAAWVARVEGYPADAGPTGAAWLATVPRLIDEALQRWG
                     LVVDGEPRTGWTAVVLPVRRDGEALALKVTWPHPEGTHEALALRTWAGSGAVRLVAAL
                     PTDGVLLLERLDADRDLSEVWVDEACEVVGGLLRQLQVPAPPQFPRLPEYLAPHLERM
                     PGRAAVPRRVVTRTVGLARELLSGEVPEVLLHTDLHFENVLMGSQGWTAIDPKPVAGH
                     PAFDLFPLLHNRVDELGTGAAFRWSVRHRVSLAAEAAGIDLDEAFAWTLLRAGIEVSW
                     VSETGDAADVSLHVALTKALDE"
     gene            15763..17781
                     /locus_tag="PL014_01795"
     CDS             15763..17781
                     /locus_tag="PL014_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT55855.1"
                     /translation="MANRLAHSLSPYLLQHADNPVDWWEWGDDAFAEAERRDVPILLS
                     VGYAACHWCHVMAHESFEDEQVAAAVNGRFVAVKVDREERPDVDAVYMSATTALTGHG
                     GWPMTCLLTPSGDPFFAGTYLPRAQFLQLLDGAAHAWETDRERVLSSGATVAERLREL
                     TGPPAPTVIDPDVCDTAVRRLRSTYDEARGGFGGAPKFPPSMVLEFLLRHHARTGSAD
                     ALAMVTGTAHAMARGGLYDQLGGGFARYSVDADWVVPHFEKMLYDNALLLRVYAHLWR
                     ATGDDLALRVAHETADFLLRDLRTDQGAFASALDADTVVDGHSHEGLTYAWTPAQLVE
                     VLGPDDAERAARLLGVTASGTFEAGASTLQLREDPDDLPWWARVRARLLAARATRPQP
                     SRDDKVVTAWNGLAIAALADAGVLLDRPDLVTAAEVAAAHVLDTHDVPASDSADDGDR
                     MLRRTSRAGRTGPAPAVLDDHANLAEGLLALHQATGEPRWGEAAASLLDRALDRFVDA
                     DGTVHDTAHDAPALFSRPANRSDNAEPSGASALAGALLTHGALTGSAAHLQASRAALD
                     ACGPVAAQDPRFAGWALAVAEASAAGPLQVAVVGEGPAAQALLRTARASTSPGLVVMH
                     GMPDAPGRALLADRPLVDDGPAAYVCRGFVCDRPVTDTEALGAALRSR"
     gene            complement(17768..18550)
                     /locus_tag="PL014_01800"
     CDS             complement(17768..18550)
                     /locus_tag="PL014_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT55856.1"
                     /translation="MTTALTTTVAAPCSPVLARRAFVDQVMGMPVSVHVRAVDTSRSD
                     VEAAAARVFAHLHRVDAVLSTWRADSDLLRVQHGEVDGVDEVHPWLADVTDLCLEAED
                     RTDGLFRAWRRRPGGRLSFDPTGLVKGWAVAAAAEHLRIVPEIAYSIGAGGDVVVGTG
                     TGVDEAPPWRIGIEDPRQRGGLARVVTVTRGAVATSGAAIRGGHVVDPRTGQGIVRPG
                     SATVVGPDLMWADVWATAAWVDPERARALIEQRDPAYGLIVI"
     gene            complement(18547..19047)
                     /locus_tag="PL014_01805"
     CDS             complement(18547..19047)
                     /locus_tag="PL014_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT55857.1"
                     /translation="MRRITLWLLSTISTLVLLFSYSTSTSASASVAASVVAEADTGSD
                     GTDTPTQPDTSTQTPAQTESSAASGSSTSTAARTILGDAIMTRYGNVQVQITVANGAI
                     TASEVTQVPWSNGKDQQINGRAVPVLNAEVVDAQSAGIDMVSGATYTSEAYIRSLQSA
                     IDRANL"
     gene            complement(19076..20530)
                     /locus_tag="PL014_01810"
     CDS             complement(19076..20530)
                     /locus_tag="PL014_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HOT55858.1"
                     /translation="MSSSTVTTSRMPSAHVRGASHLSRPTGPTRRRRLGPVPRRWRDL
                     SAALGWGVVLFTIALWVGGGGIQELGTTAGFLTSTGRLTGLVASALLLLQVGLMARIP
                     WVEQAWGQDELTRVHRLVGFTSFTLMLAHIALITLGYAASTDAGVWGTLVDFVVTYPG
                     MLLAVAGTLALVMVVVTSVKKARARLRYESWHLIHLYAYLGAGLALPHQLWTGAEFLS
                     STLATVFWWGLWASTAGAVIIFRVLLPLWRSFAGSLRVVDVRAEGPDATTVTVAGRSV
                     RRMPVRAGQFFQWRFLDGPGWSRANPYSISAAPDGATLRFTAAHVGDGSRRLATLRPG
                     TRVMVEGPYGRLHDGVRTQRKVLLMGSGIGITPLRAILEDLPQGPGDVTVVHRVRSRS
                     EAVLADELADLAAARGARYLLIEGPRITSRASWLPEQARDLSDDQALREIVPDIADHD
                     VYLCGADGWMTAARAAALAAGVPAGRVHLERFTS"
     assembly_gap    20594..20693
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20870..22714
                     /locus_tag="PL014_01815"
     CDS             20870..22714
                     /locus_tag="PL014_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HOT55859.1"
                     /translation="MFRARVARWWPDLVNGLRMAYGDERAGALGADLVELAGAAFAAR
                     SDRLHVRDLERMLRPDWLQDPSMVGYAAYTERFAGDLRGVADHLPYLDELGVRYLHLM
                     PLLRPREGDSDGGYAVADYRSVRADLGSMDDLEHLAARLHDAGMSLVLDLVLNHVARE
                     HEWAVKARAGDPTYREYFHVFPDRDMPDAYEATLPEVFPDFAPGNFTWDEDLQGWVWT
                     TFNEFQWDVNWSNPAVLREYASIILDLANRGVDVLRLDAIAFLWKRLGTDCQGEPEVH
                     ALTQVLRAVTRIACPAVAFKAEAIVAPTKLLPYLGTGAYSGKVSDLAYHNSLMVHVWS
                     MLASQDVRLAAHALGSLPPKPTTATWLTYLRCHDDIGWAIMDEDAGAVGLSGHAHRSF
                     LWHWYAGDHFGSPARGLVFQYNPETDDGRTSGTAASLIGLQSAGSAAEVESAAAALRL
                     ANAMVLGWGGIPVIWSGDELGQPNDPRWALEPGHGDDNRWAHRPRLDWERAEHRHDRT
                     TTAGRVFGDLVELVRARGSLVHLHGSVETQIAPVDDPGVLVTVREHPVGRFVGVYNVT
                     PQWRMWPGWRVNELGVADAVDRITGDALPWGGDGNVWLPPFAVLWLTT"
     gene            complement(22814..23593)
                     /locus_tag="PL014_01820"
     CDS             complement(22814..23593)
                     /locus_tag="PL014_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOT55860.1"
                     /translation="MSDPGVEVIALLGLAGFLAGWIDAVVGGGGLVQLPALLLGVPGA
                     TPAQILATNKFGSIWGTATSSVTYYRRVKPDLRTALPMAGVAYVGAVVGALIGLHIPK
                     AAFYPIILVLLIAVGAYTLFKPSLGESTALRFSGRRHTVFAMLTGFVIGVYDGALGPG
                     TGSFMVFALVGLMGYAFLEASAKAKIANFATNLGALTVFIPGGHVMWVVGGVMAVANL
                     LGGYVGARTAVAKGSGFVRVVFIIVVGAFIIRIAGDLLGAW"
     gene            complement(23601..24356)
                     /locus_tag="PL014_01825"
     CDS             complement(23601..24356)
                     /locus_tag="PL014_01825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT55861.1"
                     /translation="MSHPVDAVVFDWGGTLTPWHEVDLPEQWRVFAREVHGIPVDSPD
                     VSQADLDEAHRLADRILEVESAAWTRGRDEHTSAALADILEAAGIDAAHDRHHLALAA
                     YRGFWEPHTHTDPQVRPLWEGLRARGIKVGVLSNTIWSREYHQGIFERDGVLDLVDAD
                     LYSSELDHVKPHPEAFRAICRELDVDPRRSVYVGDRIFEDVHGPQQVGMRAIWIPHSD
                     IPANQRVEVTAVPDGRAHELLDVLALVDAWMAA"
     gene            complement(24353..26122)
                     /locus_tag="PL014_01830"
     CDS             complement(24353..26122)
                     /locus_tag="PL014_01830"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT55862.1"
                     /translation="MAMRMSSLFLRTLRENPADAEVPSHQLLVRAGYIRRVSPGVYTW
                     LPLGLKVLRKVETIVREEMDAIGSQELLFPALLPREPYEASGRWTEYGENVFRLVDRK
                     GADHLLGPTHEEMFTLTVKDLYSSYKDLPLSIYQIQTKYRDEARPRAGILRGREFVMK
                     DSYSFDIDGAGLDKSYQLHRDAYVRIFDRLGFHYVIVQAMAGAMGGSKSEEFLATAEN
                     GEDTYVRCVNCGYAANVEAVRVPTPDAVDVTSLPAAHVEDTPDTPTIDTLVAAANADH
                     PRADGRAWTAADTLKNVVVMLVLPDGTREPLAIGVPGDREVDEKRLGAQVEPARVEAF
                     EEKDFANYPTLAKGYIGPGALGLEKSTGIRYLLDPRVADGTAWITGADISGKHVFDLV
                     KGRDFEGDGTIEAAEVRDGDACPSCGHGLESARGIEMGHIFQLGTKYAEALDLKVLDE
                     NGKLRTVTMGSYGVGVSRAVACVAEGNHDELGLIWPRALAPADVHVVVTGKDETVATY
                     GEELTRALEARGVAVLLDDRRQASPGVKFKDAELIGVPTIVVIGRGLAQGVIEVKDRR
                     TGERREVAVDAAVQEILAEIGQA"
     gene            complement(26306..27100)
                     /locus_tag="PL014_01835"
     CDS             complement(26306..27100)
                     /locus_tag="PL014_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT55863.1"
                     /translation="MSPVVLLLAVLVGMALGLLGGGGSILTVPLLVYIAGLDAKEAIA
                     TSLLVVGVTSLAALVPHARAGRVRWRTGAIFGAAAMAGAYAGGRIAEFIPGTVLLVAF
                     ALMMVATAVAMIRGRARQDAAGPGSGSAAGDAGGSASVRELPVKLVLLEGVVVGLVTG
                     LVGAGGGFLVVPALVLLGGLPMPVAVGTSLLVIAMKSFAGLAGYLSSVSIDWGLAAAV
                     TAMAVLGSIGGALLSGRVPQDRLRQGFGWFVLVMGVLVLGQSALTA"
     gene            complement(27117..28019)
                     /locus_tag="PL014_01840"
     CDS             complement(27117..28019)
                     /locus_tag="PL014_01840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT55864.1"
                     /translation="MSTLDWKPLTEGDLPALTHLAQCCLERDGGLPLLATEPMLRQLF
                     LSGHSVGGRDETGELIAAAGAFLDASATRSVTALVHPSVRSQGIGEQLLKWWLAQAGG
                     GPMRVVAETASPESDAFAARLGLERTFAEHVMRRPLVEVPRIVRPAEVHTYPWTADTQ
                     YLFHAAYSRSFANRPGFPDTPFAEWVRSVEEDEGFHPDLSRVALDEDGHVAGFVTISD
                     NWIDQVGVVPEWRGQRLGAHLVVRSLRTLRKAGYEQAWLAVNVDNPSARALYRSLGFL
                     DSGVRARYQQVGPIQGKDVPSDIP"
     gene            complement(28105..28950)
                     /locus_tag="PL014_01845"
     CDS             complement(28105..28950)
                     /locus_tag="PL014_01845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT55865.1"
                     /translation="MLRTRSPIRPLSLADRDDALDLCSRDLPGSVFVAARILEGARTG
                     SLMSVLGHRVDGELTSLCWASANVVPVATTPESQALIAERVRRWKGRCASLFGRRDEV
                     EGLWSHLAPHWGPARAIRTRQPLLVADRLPSTLGVQIDQRVRPARVDEVDLVLPAAEH
                     MFTAEIGYPPYRGSSRGYLASLATLAQRGHTYVVIEHGEVVFKTDIGSLALGCAQLQG
                     VWLAPHLRGQGLAAPMLASVVEQVMLGPAPLVTLYVNDFNTAARATYRRLGFRDAGDF
                     ATVLL"
BASE COUNT         4022 a        10185 c        10504 g         4168 t
ORIGIN      
        1 cctcgcgacc gccttcaccg aaggggtcca ggccaccctc gacgagttcg acgtgccgtg
       61 gtcggtgagc cagctcgggg cgcgctcgga gtaccgcttc gcctcaccgg cgccgcgcac
      121 cggcgaggag gccgcgatcg cccacgacga cgacctcgac gcgtacatgc acctcgcgat
      181 gtgcaaccgc ggcatcctca tgacgccgtt ccacaacatg gcgctgatgt gcccggagac
      241 cacccgggcc gacgtcgacc ggcacacggc caccttccgc gaggtcgtcg ccgccctcta
      301 cgcctgatcc accccccacc ccgcgactta ttgcactcca gccacccgga tccgggtggc
      361 tggagtgcaa taggtcgggg tgtgtcagtt gccgaagcgg tcgcggatgg cgacgagctc
      421 gacggcgtgg gcgtccaggg cgcggtcctc gtcggtgacg ggcgtccacg tggggcaggg
      481 cagcgcgcgt cgctgctcgt cgatgccgac gaagaccgtg cggcagaagg tcgtcagctc
      541 catgccgtcc gcgccggcag tcgggtcacc cgagcgcacg tgcaccccga tgtgcatcga
      601 cgtcgtgccg gtgtgcagca gccgggcctc gacctcgacg acgtggccga tccgcagcgg
      661 tcggtagaac cgcaccccgc cggtgaagat cgcgacgttg tgtgcgttcc ccgtccactg
      721 ggtcgcgagc acgtgggcag cctcgtcgat ccagcgcatg acgatgccgc cgtggacctt
      781 gccgccccag ttgacgtcgg tcggtgccgc gaggaaccgc agcacgacgc gaggcgccga
      841 cccggcatcg gtgtactgct ccctcgccat cgccgcctcg acctcccggc gcaacgcgac
      901 gcggttcatc gcccactgct gctcgagctc ttcgccgagg gtgcgcggca cgagctcgcg
      961 aaccggcgtc gaggaaccct tgtcgtcgac cgacacgaag atcatcaggc accgggtggc
     1021 tggctccagg gcacgggtgc ccggctgacc agaggcgacg tcgacggtga tgtgcatcga
     1081 cgtgcggccg gtgtacacga cgcgggcgct cacctcgacg agctcaccga cctcgacggg
     1141 ccgggtgaag cgcacgttgc cgacgtacgc ggtgacggca tacccgccgc tccaccccgc
     1201 cgccgcggca taccccgcct tgtcgatcca ctcgagaacg cggccggccg acaccgaacc
     1261 cgactgcccg gcatccgtcg gggcggcgag cagccgcagc gtgatctcgg cgcggtcggc
     1321 tgcgggatcg ggcatgacgt caggcgtggc tgtgctcggc gaactcggcg cggccggcgc
     1381 cgaggacggt aaggggcagc aacgacttcc cggtggggcc gatctcgatc tgcgtgccca
     1441 tctgggggca gaccccgcag tcgaagcagg gcgtccagcg gcagtcctcg acctcggtct
     1501 cgtcgagcga gtccagccag tcctgccaga gccattcctt gtcgagcccg gagtcgatgt
     1561 ggtcccaggg cagcacctcg gcctcctcgc gctcgcgcgt ggtgtaccag gcgacgtcga
     1621 ccccgaaggc cgacagctcg cgctcggcgg cggccatcca ccggtcgtag gagaagtgct
     1681 cggaccaccc gtcgaagcgg gcgccgtcac gccacacggc ctcgatgacc cgcccgaccc
     1741 ggcggtcacc gcgcgagagc aaaccctcga cgatgcccgg cttgccgtcg tggtagcgga
     1801 agccgatggc cgagccgtag cggcggtcgg agcggatcgc ctggcgcagc ttgtcgagcc
     1861 gcgcgtcggt ctcgtcggcg ccgagctggg cggcccactg gaacggggtg tgcggcttgg
     1921 gcacgaagcc gccgatcgag acggtgcagc ggatgtcacg gcgaccgctg acctcacgcc
     1981 cggtgtcgat gacccgcttc gcgagctcgg cgatctgcag gacgtcctcg tcggtctcgg
     2041 tcggcagccc gcacatgaag tagagcttca cctggcgcca gccggcaccg tacgcggcgg
     2101 cgaccgtgcg gatgaggtcg tcctcggtga ccatcttgtt gatgaccttg cggatgcgct
     2161 cggagccacc ctcgggggcg aaggtcaggc ccgaacggcg accgttgcgg gtcagctcgt
     2221 tggccaggtc gatgttgaag gcgtcgacgc gcgtcgacgg cagcgacagg ccggtgttcg
     2281 tgccctcgta acgatccgcg agccccttgg cgaccgacgc gatctcactg tggtcggccg
     2341 agctcagcga gagcaggccg acttcctcga agccggtggc ggccaggccg cgctcgacca
     2401 tctcgccgat gccggtgatg ctgcgctcgc gcacggggcg ggtgatcatg cccgcctggc
     2461 agaaccggca cccacgggtg cagccacgga agatctcgac cgacatccgc tcgtgcaccg
     2521 actcggcgag cggcacgagc ggctgcttgg ggtagggcca cgcgtcgagg tccatgacgg
     2581 tgtgcttggc cacccgccac ggcacctgcg ggtaggccgg gttcggcgcg atccgctgga
     2641 tgcggccgtc gggcaggtag ctgacgtcgt agaactgcgg cacgtagacc ccgcccgtgc
     2701 gcgcgaggcg aagcaggatc tcctggcgac cacccggcat cccctcgcgc ttccacgcgc
     2761 cgacgatgtc ggtgatcgcg agcaccgcct cctcgccgtc gccgaccacg gcggcgtcga
     2821 tgaagtcggc gatcgtctcg gggttgaagg cggcgtggcc gcccgccacg acgatcgggt
     2881 ggctctcgtc gcgatcggca gcgtgcagcg ggatgccggc gaggtcgagc gcggtgagca
     2941 tgttcgtgta gccgagctcg gtggagaacg acaggccgaa caggtcgaag tcgccgacgg
     3001 ggcggtgcgc atcgacggtg aactgcggca cctggtgctc gcgcatcagg gcctcgagat
     3061 cgggccagac cgcataggtg cgctcggcga gggcgtcagc ccgctcgttg agcacttcgt
     3121 agaggatcat gacgccctgg ttgggcaccc cgacctcgta ggcgtccggg tacatcaggg
     3181 cccagcgcac ggtgtgctcg ccaccgacgt cccacggctt gacggtcgag ttcagctcac
     3241 cgccgacgta ctggatcggc ttgctcacct gctcgaggag tggctcgagg gagcggaaga
     3301 tcgactgacc gggcatcccc caagagtagg cgggaggcgc tacccgccca aacgagtggg
     3361 cagcagccgg gcgtgcgggg gcacactgga gatgtgagcg gcgagcgagc ggtgggcaac
     3421 cccctcggcc cggctgcggt gcgcgacgcc atggcggccc agccccgcgc cgggttcctc
     3481 ccgcgggcgg tgcgccagcg ggccggcttc gacgggccgc tcgacatcgg gcacgggcag
     3541 accaacagcc agccgcgtac cgtcgccgcg atgctcgagc tgctcgacgt gcgcccgggg
     3601 caccgcgtgc tcgacgtcgg ctccggatcc ggctggacca ccgcgatcct cgcgcagctc
     3661 gtcggccccg ccgggtccgt gctcggcctg gagctcgtgg ccgacctggt ggagcagggg
     3721 tcgtcgaacg tcgcgcgggc cggggtgccg tgggcgagga tccgcgcggc cgacccgtcg
     3781 cggctgggcg ctcccgacga ggcgccgttc gaccgcatcc tcgtctcggc gatggccgac
     3841 acgatgcccc gagagctcga ggaccagctc gcggcggacg gcatcctcgt ggtccctgtg
     3901 gcgggttgga tgctgcgggt acgccgggat gccgccgcgc ccggtggcgt tgtcgtgacc
     3961 agtcacgggg cgtaccgctt cgtccccctg gtcacccact gacacccagc cgccgtgccc
     4021 acggcggtgc agcggccagg tcgtgggacg acggcagcac cccggctccg tgcggagccg
     4081 gggtgctgtg acgacggtcg ggatcagccc gtgtagtggg cgtccagccg gtccttgtac
     4141 ttgtcggcca cgaccttgcg cttgagcttc atgctcggcg tgagctcacc ggcctcgatc
     4201 gtgaggtcgt tctcgaggat cgtgaacttc ttgatcgtct cccaccggtt gagcccggcg
     4261 ttgagctggt cgatgtagcc ctgcaccatc tcgcggcacg cgtcggagct gacgatctcg
     4321 gtgtaggact tgccctccat gccgttggcc gcggcccagc cggccatcgc gtcagggtcc
     4381 agggtcacca gggccgagac gaagttgcgg tcggcgccgt ggacgacgag ctggctggcg
     4441 tagggacaca ggcccttgaa cgtcgactcg atgatcgagg gggcgacgta cttgccgttg
     4501 ctggtcttga acaggtcctt cttgcggtcg gtgatccgca ggaagccacg ctcgtcgacc
     4561 tcaccgatgt cgccggtgtg cagccagccc tcggagtcga tggtctcggc cgtcgcgtcg
     4621 gggttgttgt ggtagccctc catgttgccc ggaccacgca gcagcacctc accgtccgcg
     4681 gcgatctgca cctcggtctt gggcagcggc cagccgaccg tgccgtagcg gtagccgcgc
     4741 gggcgggcca cgaacgaggc cgcgctcgtc tcggtcatcc cgtacccctc gaggatctgc
     4801 atccccatgg cgtcgaacca gcgggcgacg tcgttgttca gggccgccga gccggagatg
     4861 aagaagcgga cgcgtccacc gaaccggtcg cggatcttgg tgccgatgac cttgtcggtg
     4921 aaggagtgct tcagcgccag catgcccgat gggttcttgc cctgctcgcg cagctcgctg
     4981 acctcgcggc cgacgtcgct ggccaggtgg aagagcttgg ccttcacccc accctcggcc
     5041 tccatcatca tgctgatccg gccgtaggcc ttctcgaaga tgcgcggggc ggcacccatg
     5101 aacgtgggct tcaccacggc caggttgtcg acgatcttgt cgatgcggcc gtcgatggcc
     5161 gtcgggaaac cgatctgcag gggcagggtc agcagcacct tgccgaagac gtgcgcgagt
     5221 ggcagccaca ggtactgcag gtcgtcttcg ttgaggatgt gcgtgctggc gaccgccgca
     5281 ccctcgtacg tccaggcgct gtgccgcagg cggacaccct tcgggcgacc ggtggtgccc
     5341 gaggtgtaga tgatcgtcgc gagctggtcg tgctggatgc cgtcgatgcg ggcgtcgatg
     5401 gcccccgggt cgccggcgag gtactcacgg ccgagctggc ggacctggtc gaggttcagc
     5461 gtccaggggt cgttgtcgtc gacctcggcg tcgaacacga tgaccctggt cacggccggg
     5521 gtgctcgcgc ggatgccgcg cagcttgtcg agctggtcgg cgttctccgc gaagatcacc
     5581 ttggtgccgg agtcggcgag gatgaacgcg acgtcgtcgg ccatcgtcgt cgggtagatc
     5641 gtggtggtcg cacctccggc gaccatcgtg gccaggtcgg caagggccca ctcgtacctg
     5701 gtgctggagg cgatggccac gcggtcctcg ggctcgacac cgagggcgat gaggccggca
     5761 ccgagctcgc gaaccacgac gtcgacctcg gcccaggtgt cggtgaccca ctcgtcaccc
     5821 tcgggcttga cgttggcgta catgaaggcg ggcccgttcg ggctcgccgc aacgcggttg
     5881 cggaagaggt gcccaacgct ctgggcctcg ttgtcgaaga tcgcctggtc gacgggctcg
     5941 tcggcggggg tgttgatctg gggggcagct gtgggcatgt cgtacatcgt cctcgtcatt
     6001 gggggccggt ggtgccgaat cctgtcacat ggacgtgccc gacggaacgt gttgccaatg
     6061 gttgttaccg gtgagttgcc ccacgaacgc gctcacggca tccggacctg cctgaacccc
     6121 gacgaacgtg ggtgaagatc gtcgtccagc cgacgatctt cacccacgtt cgcgagggac
     6181 cgcgtggcgg gacggaaccc gggctcagtc cggcacgatc ctcgccagca gctcacggca
     6241 ccgcgcgaca tcgctggcca tggcctcggt gagctcatcg acgctgtcga agcgcagtgt
     6301 ggggcggatg tgggcggtga actcgaccat gacgcgctcg tcgtagaggt cgaggtcgtc
     6361 gcggtcgagc acgtaggcct cgaccgtgcg ccggtcctgg gtctcgaacg tcgggttcgt
     6421 gccgacgctg atcgccgcag gcatggtgcg gtcgggctcc ccgggcgagc ggtccaggcg
     6481 ggtgagccac ccggcataca ccccctcctg gggcaccagg ccgagcgagt ccgcggacag
     6541 gttggccgtc gggtagccca gcccccggcc ccggtggttg ccgtggacga ccgtgcccac
     6601 gacccggtgc gggcggccga ggatgcgcgc ggcctcaccg acctcgccag cccgcaggtg
     6661 ggtccgcacg acggtcgagg agaaccgctc gccgtcaccg acgtcgtcga gcgtgacgac
     6721 ctcgaacccg aacttctccc ccaggtctgc cagggtcgag acgtcgccgg agttgcggta
     6781 cccgaaccgg gtgtccatcc cgacgacgac gcagcgggcc gacagcgcgc gcacgaagac
     6841 cgcgcgcacg aagtcctccg gctgctgctt ggcgaactcc tgcgtgaagt ccatgacgag
     6901 gagcccgtcg atgccggtgt cctcgatgag gtcgtcgcgg atctcgcccg gggcgatgag
     6961 ctcgggggcg gactcggggc gcagcacggc gaccgggtgc gggtcgaacg tcaccgcgac
     7021 cgacggcaga ccgagccggt gtccggactc gatgagggcg ttcagcaccg cgcggtgacc
     7081 gcggtgcacc ccgtcgaagt tgccgagggt gacgacacag ggcctcaggg cctcaggtgt
     7141 ctctgacggg tcggaccaac ggtgcacgcg cacactctac gaagtcgagg gcgagaacac
     7201 gacgaggctg cgtgccgtgg gacgggactc gtccaacatc gccacgagtc ggccgtccgg
     7261 ggcgatggcc gcgaccacct cttctcgacc cggcacctcc gacggcagcc gctgtccgaa
     7321 gccgatggtg cgcgcctgcg cctcgtcgac ctcacgcacc gcgaacgcgg cgcgagccgc
     7381 ctgcgcgagg ggcgtgaccg gcatccgctc accctgacga gcctccaggt cagccaacgg
     7441 gtgcgcgtcg tcgagggtga agccgccgac gcgggtgcgg cgcagcgccg tcaggtgccc
     7501 accggtgccc agggccgcac cgaggtcgcg cgcgagggca cgcacgtagg ttccggacga
     7561 caccgtgacc tcgacgtcga cgtcgagcac cgccgtgccg tcgacctcca gggctcgtac
     7621 gtcgccgatc tcgaagcggg agacggtgac cgggcgtgcg ggcaggtcga ccgactcccc
     7681 cgcgcgcacc cggtggtagg cgcgctcacc cttgaccttg atcgcgctca cggcgctcgg
     7741 gacctgctcg atgtcgcccg tcagggtcgc ggccgcgacc cgcaccgcat ccgcctcgat
     7801 ccccgaggcg ccggcggatg ccgtgacctc gccctccgcg tcgtcggtca cggtcgcctg
     7861 gccgagtcgg atcgtcgccg tgtagtcctt gtcgcacccc acgaggaagg tcagcagccg
     7921 tgtcgcccgg ccgaccccga gcacgagcac acccgtggcc atggggtcga gcgtgccggc
     7981 gtgccccacc ttgcgggtgg cgcacagccg ccggccacgc gcgacgacgt cgtggctggt
     8041 ccagcccgcg ggcttgtcga cgacgaggat gccgtcgggc gtggggtcag cggtcgtcgt
     8101 caccgacggc ttcgtccacg tcgtccgcgt cgtcgccgtc gacgtcgtcg tcctcgtcga
     8161 cctcgcggtc cttcttgtac gggtcggcgt ccccggcgtg cacggcaccg gcagcggccg
     8221 cagccacggc ggcgtcgcgc tccttggcct cgcgcaggag gtcctcgagg tgcgccgcgg
     8281 tctccgggat cgcgtcggcg atgaactcca gcgacggcgt gagtcggatg ccgagctgct
     8341 tgccgacgaa cgagcgcagc ttgccgcggt tctgctcgag gaggcgggcg gtctgcgcgc
     8401 gctgggcggc atcgccgaac acggtgtaga agagcgaggc gtgctggagg tcaccggtga
     8461 ccttgacgtc cgtgatcgtc acgaacccga ggtcggggtc cttgacgatc gcgtcgaggt
     8521 tggctgccgt cagcgtcttg atgcggtcgg cgatcttctg ggcgcgtgcg gtgtcggcca
     8581 cggctcctcc ttgaggtcct cggatggggc ggcacccacc ctagtgacgg gtgccggtga
     8641 cggcgccggg cggcatccga tcgttcggat gccgcccggt cgcgggtggt gctgcctcgc
     8701 gtcaggcgcg aggcttctcc cgcatttcgt aggtgctgat gaggtcatcg agctgcaggt
     8761 cgttgaacga cccgaggttg ataccgcact cgaacccgtc gcggacctcg gtgacgtcgt
     8821 ccttgaaccg tcgcagaccg gcgatctcga tgttctccgc gacgacgacc ccgttgcggg
     8881 tgatccgcgc ccgggtgccg cgcttgatct cgccgctgcg cacgatcgag ccggcgatgt
     8941 tgccgaactt gctgctgcgg aagatctcgc ggatctgcgc ggtgccgagc tcgacctcct
     9001 cgaactccgg cttgagcatg cccttgaggg cctgctcgat ctcgtcgatc gcctggtaga
     9061 tgaccgagta gtaccggatc tcgacgccct cgcgctcggc gacctcggcg ttctggccct
     9121 cggcccgaac gttgaagccg atgatgatgg cgttggaggc catcgcgagg ttgatgttgt
     9181 tcagcgtgat ggcgccgacg ccgcggtcga tgatccgcag gtcgacctcc tcgccgacgt
     9241 cgatctggag cagggcgtcc tcgagcgctt cgacggaacc cgacacgtca cccttgagga
     9301 tgaggttgag ggtgtcgacc ttgcccgccg cgagggcctc gttgaggtcc tcgagggaga
     9361 tgcgcttgcg ggccttggcc aggctggcct gacggtcggc tgcttcacgc ttctcggcga
     9421 tctgacgtgc cgtgcggtcg tcgggggcga cgacgaaggt gtcgccggcc cgcgggaccg
     9481 aggacagacc gagcacctgc accggacgcg acggggtcgc ctcggtgagc gcgttgccgt
     9541 gctcgtcgag catggcccgt acacggccgt gcgaggagcc ggcgacgatc gcgtcaccga
     9601 cgtgcagcgt gccggactgg atgagcacgg tcgccgtggc gccacgcccc ttgtccaggt
     9661 tcgcctcgat ggcgatgccg cgtgcgtcgc ggtcggggtt ggcccgaagg tcaagggccg
     9721 cgtcggcggt gagcaggact gcctcgagca gctggtcgat gttctgaccc tgcttggccg
     9781 agacgtcgac gaacatcgtc tcgccgccgt actcctctgc cacgaggttg tactcggtca
     9841 gctgctggcg gatcttgctc gggttggcac cctcgacgtc gatcttgttg accgcgacga
     9901 cgatcggcac gtcggccgcc tgggcgtggt tgagcgcctc gatggtctgc ggcatgacgc
     9961 cgtcgtccgc cgcgaccacg aggatcgaga tgtcggtgac cttggcacca cgggcacgca
    10021 tggcggtgaa cgcctcgtga cccggggtgt cgatgaaggt gatcggccgg tcgacgccct
    10081 cgtgctcctt gtggatctgg taggcgccga tgtgctgggt gatgccgccg gcctcgccgg
    10141 cagcgacctc ggcgttgcgg atcgcgtcga gcagacgcgt cttgccgtgg tcgacgtgac
    10201 ccatgaccgt gacgaccggc gggcgggcct cgaggtcctc ttcgtcctcg gagtcgacac
    10261 cgatctcgag gtcgatgttg aaggagtcga agagctcgcg ctcctcctcc tccggggaga
    10321 ccatgcggat gtcgtagccg agctcgctgc cgaggagctt gaaggtgtcc tcgtccaggc
    10381 tctgggtggc cgtggccatc tcaccgaggt ggaacagcac cgtcaccagc gaaccggggt
    10441 tggcattgat cttgtcggcg aagtcggaca ggctcgcgcc ctgacggatc tggatgatcg
    10501 tcgtgccgtc accacggggg acgctcacac cgccgatcga cggtgcctgc atctcctcga
    10561 actccatgcg cttcgcgcgc ttggacttgc gggcacgacc ggggcgtcca ccgccacggc
    10621 cgaaggcacc ctgggtgccg ccgcgaccac cggggccgct gcggaagcca ccggggccac
    10681 cgggacgtcc ggcgaaaccg ccgccaccac cgggtcgcgc acctgcgcca ccgggtgcac
    10741 cggggcgacc ggcgccacgc gccggacgct cacccggacg gccgaccgcg gagcggtcgg
    10801 gcatcatgcc ggggctgggg cgcgggccac cggggcgcgg accggcagca ccggcacctg
    10861 cgccaccggg gcgaggacca cccggacggc tctgcggccg gggcatgccc tggctcggcg
    10921 cgaaggggtt gttgccgggg cgggcgggct cgcgggtgcg gcccatcccc tggctcggcg
    10981 cgaacgggtt gttgcccggg cgcggagtgt tcggacgggg cgcgccggga cgcggtgcac
    11041 cctggccgga gccaccctcg cgggcggcag gagccgccgg ggctgccggt gccggggcag
    11101 ccggagccgc cggggccggt gcaggcgccg acggggccgc gggggcaggt gccgccggag
    11161 ccggggttgc cgccgccggt gctgccgggg cagcaggcgc cggtgccgac ggagcggcgg
    11221 gagccggtgc cgacggggca ggcgcagcgg gagcgggggc tgccggagca ggcgtcgccg
    11281 cagccggtgc cgaaggggct gccggggccg gacgcgccgg agcagcagca ggtgctgccg
    11341 gggcagcagg tgcctcggca gggtacttct cgttgtagcg ccgaaccacg ggggcttcca
    11401 ccgtcgaact ggcgctcttg acgtactcgc ccatgccgtt caggcgctcg agaaggacct
    11461 tgctggtcac tccgagctcc ttggcaagtg cgctcacacg gactttagac acttttctcc
    11521 tgtccaggtc cgtgcgtcgt gcgcggaccg tcgttacagc tggggggtgc tcatcgctga
    11581 gtactcatcg ggtgctcatc agcgtcaaac ccgctttcgg tttcatgctc ggacggtggt
    11641 tcctgcaggt gggccctgac ccagtgctcg acggcactgt ggtcggcggg tgtgctcgtt
    11701 cgcaacgccc gaccgaacgc ccgccgacga acggcgagct ccaggcacga gatccccggg
    11761 tgcagccatg cgccgcgccc cgggagacgg cgctcaggat cggggagaac gacagcgatg
    11821 ccgtccgcgc cggtcccgag gaccactcgc aggagtgccg accggctatc cgtcccacga
    11881 cacccgatac aggtgcgtat cgggtggccg ggccggagtc cagtccgatc ggtagccacc
    11941 agcctaccgc ctaggtgccc tcggaggcgc catcggcgcg ggagggggcc acggcatccg
    12001 ccgctgcggg gtcggtgtcg ggtcggatgt cgatccgcca gccggtcagt ttggcggcca
    12061 ggcgggcgtt ctggccctcg cggccgatcg ccagcgacag ctggtagtcc ggcacgacga
    12121 cacgggccgc gcgcagcttg gggtcgacga tcgtgaccga gttgacccgt gacggggaca
    12181 aggccgcggc gatgaacgtc gccgggtcgt tcgaccagtc gacgatgtcg atcttctcgc
    12241 cgtgcagctc ggccatgacg gcgcgcacgc gggcacccat cggcccgatg caggcaccct
    12301 tggcgttcag gccggcgacc gtggtgtgga cggccagctt ggtgcggtgc ccggcctcgc
    12361 gcgcgagggc ctcgatcgtg acgctgccgt cggcgatctc gggcacctcg agggcgaaca
    12421 gcttgcgcac gaggttcggg tgggtgcgcg agagctcgat ctgcgggccg cgcatgccgc
    12481 gcttgaccga ggtgacgtag cagcgcagtc gctgcccgtg gacgtacttc tcccccggca
    12541 cctgctcggc gaggggcagg atgccctcga ccgtgccgaa gtcgacggtc acgtggcgcg
    12601 gcaccgggct ctgctggatg acgccggcga cgagctcgcc ctcgcggccc ttgaagtcgc
    12661 cgaggatggc gtcgtcctcg atgtcgcgca gccgctgcac gatgacctgg cgggcggtcg
    12721 cggcggcgac ccggccgaag ccgctcgggg tgtcgtcgaa ctcgggaccg agctcacggc
    12781 ggggacgcac ggggttgccc tcgtcgtcga ccgcgccctc ctccgcgggg acctcgagct
    12841 cctcacgcgc ccagatgacg acgtgcccgg tcttgcggtc gagctcggcg cgcgagaccc
    12901 ggtagtggcc ctcggtgcgc tggtaggcga gcgtgatcgc ctgctcgatc gcctggatga
    12961 ggacatccat ggcgatgcct cgctcgcgct cgagcgcgcg cagtgcggtc aggtcgatgt
    13021 ccatgtcagg tgtccttgtc gtccggtcgg gcgaactcga cctgcaccgc ggcacgttcg
    13081 agaccggcat acgccagggt ctcggtgcgc gagggggtcg agcgggtggc ggggatgtcg
    13141 agcgtcacgt cggtgccgct ggcacgcagc acgcggccgg tgacctcgtg gccgtcgtgg
    13201 gtggcggtga cgaggcggcc gacgttgcgg cggaagtggc gaggaagggt cagcgggcga
    13261 ccgacgccgg gtgaggagac ctcgagggtg tagggctgct cacccatgag gtcgctcgcg
    13321 tcgagggcgt cgctgaccgc gcgcgtggcg tcggcgacct cgtcgagggt gaggggatcg
    13381 gtcggctcgt cggcgacgtc ctgcgggccc gggtcgcggt cgacgaggac cttgacgacg
    13441 cggcgccggc ccgcgggggt cacggcgacg tcctcgagaa cgaggccgat gccctccacc
    13501 gcgtcctgca cggtcgggcg gatgccgtct gcagtggtca tcgtgctgcc cgtcctctct
    13561 gtgaagttag ccgtcggcgg gtcgccgccg cccaccactg taggccagat caggtgctgc
    13621 tccggcccgt gcgatgatgc gcgcatgggt gaggcggcac gtccgggggt ctccagaagg
    13681 gctcttctcg cggcctctgt gggcgcgctc gccctcccga gcgcggcgtg cggcatccgg
    13741 ctcgaggacg acgccccacg cgtgccactc gtcccgacgc gcacaccgat ccccctcgag
    13801 agcgagctcg tcgccctcac gggcgggacc ctcgccctgg ccgtcctcgc gggcaccgtc
    13861 ccgggggcgc tggccgccag cctcgcgacc atccaccggc gccagcacac ggtgttgcgc
    13921 acgacgctgc tgcgggggca ggtcccgctg tcgctcgtcg acgggcccgc tgcgtccccg
    13981 agcgcatcct ccgagtcacc gagtggctcc cccgacccgg ccacgagcgc gaccggcacc
    14041 cctgcgccgt cagccagccc gacggcatcc acggtcgaga gcctcgcgtc cgccgaaggt
    14101 gagtcggcgg tgcgtgccgc cgagttcgcc ggcgccgagc ccgacctcct cgccacgctg
    14161 gcctcgctgc acgcgcagcg cttcgcggca gcgaccctgc tgtccggcac ggccccgacg
    14221 gtcgccgggg ctgctgtcgc gggtgacgcg gtcgaggcga tggccgcgag cacgagcgcg
    14281 gcgatctacc tgttcgaggt ggtcagtgcc cggacgtccg ggcgggtcag gagccgggca
    14341 gacaccaccc tgcgggtgct tcgctcggtg cgcgctgacc aggtggccgg cggggcgagc
    14401 ctcggaagca cgctcggtca gccgctcccc ttccccgtga cgacgacggc cgatgccgct
    14461 cggctggctc gcgaggcgat gctcgcgttg cgctcggggt acggcgagca cctcgcggcg
    14521 ctggcggcaa ccggtgcaac gccgggagcg gctgcggtgt cgggagtcga tgcgggtacc
    14581 gctggggact tggccggtgg cgcggcgccg ggagatgcgg ccggtgcggg acttgctgct
    14641 gcgaccaggt ggctcggcgc cgtcgaggtg caggcgaacc gctggggtgt cgccctggca
    14701 cccttccccg gcctcacgtg acccgctcgt gagcacctcg tgagccgggt ggtgctgcca
    14761 gccgcttggg tcgctcgtgt cgagggctac cccgccgacg cagggccgac gggtgcagcg
    14821 tggctggcga ccgtcccccg cctcatcgac gaggcgctgc agcggtgggg tctggtcgtc
    14881 gacggtgagc cgcgcaccgg atggaccgcg gtggtcctcc cggtgcgccg cgacggcgag
    14941 gcgctcgcgc tcaaggtgac ctggccgcat cccgagggca ctcacgaggc cctggcgttg
    15001 cggacgtggg cggggtcggg ggcggtacgg ctcgtcgccg cgctgccgac ggacggggtg
    15061 ctgctgctcg agcgtctcga cgccgaccgc gacctgagcg aggtgtgggt cgacgaggcg
    15121 tgcgaggtgg tcggcgggtt gctccgccag ctccaggtgc ccgcgccgcc gcagttcccc
    15181 cggctcccgg agtacctcgc accacacctg gagcggatgc cggggcgggc cgccgtgccc
    15241 cgtcgggtcg tcacgcgcac ggtcgggctt gctcgtgaac tgctctccgg tgaggtgccc
    15301 gaggtgctgc tgcacaccga cctgcacttc gagaacgtgc tcatggggtc gcagggctgg
    15361 acggcgatcg acccgaagcc ggtcgcgggt caccctgcgt tcgacctgtt cccgctgctg
    15421 cacaaccgcg ttgacgagct cgggacgggg gctgcgttcc gctggtcggt gcggcaccgg
    15481 gtgtcgctgg ctgccgaggc tgcggggatc gacctcgacg aggccttcgc gtggaccctg
    15541 ctgcgggccg ggatcgaggt cagctgggtc agcgagaccg gcgacgctgc cgacgtgagc
    15601 ctgcacgtgg ccctcaccaa ggcactcgac gagtagccgc cgctccactg gggcggcagc
    15661 cgaggctggt cgagtcgacg ccgggtcgaa tcgcaggtgc acggatctgc gggcggtagc
    15721 acctcgaccc cgcggggcca cctcgcggca tacggtgggg agatggcgaa ccggctcgca
    15781 cactcactca gcccctacct cctccagcac gccgacaacc ccgtcgactg gtgggagtgg
    15841 ggcgacgacg ccttcgccga ggccgagcgt cgcgacgtgc cgatcctgct ctcggtgggc
    15901 tacgcagcct gccactggtg ccacgtcatg gcccacgagt cgttcgagga cgagcaggtc
    15961 gccgctgccg tcaacgggcg cttcgtcgcc gtgaaggtcg accgcgagga gcgccccgac
    16021 gtcgacgccg tgtacatgtc ggccacgacc gccctcaccg ggcacggcgg ctggccgatg
    16081 acctgcctgc tcaccccgtc gggtgacccc ttcttcgcgg gcacctacct gccgagggcc
    16141 cagttcctcc agctcctgga cggcgccgcc catgcgtggg agaccgaccg cgagcgcgtg
    16201 ctgagctccg gggcgaccgt ggccgagcga ctgcgtgagc tcaccggccc gccggccccg
    16261 acggtcatcg accccgacgt ctgcgacacg gccgtgcggc ggctgcgctc gacctacgac
    16321 gaggcccgag gtgggttcgg cggcgcgccg aagttcccac cgtcgatggt gctggagttc
    16381 ctcctgcgcc accacgcccg cacggggtcg gccgacgccc tcgcgatggt caccggcacg
    16441 gcgcacgcca tggcccgcgg aggcctctac gaccagctcg ggggcggctt cgcgcggtac
    16501 tccgtcgatg ccgactgggt ggtgccgcac ttcgagaaga tgctctacga caacgccctg
    16561 ctgctgcgcg tctacgccca cctgtggcgc gcgaccggcg acgacctcgc cctgcgggtg
    16621 gcccacgaga ccgcggactt cctgctgcgg gacctgcgca ccgatcaggg cgcgttcgcc
    16681 tcggccctcg acgccgacac cgtcgtcgac ggccactcgc acgaaggtct gacctacgcg
    16741 tggaccccgg cgcagctcgt cgaggtgctc ggacccgacg acgcagagcg cgcggcccgc
    16801 ctcctcggcg tcactgcgag cgggaccttc gaggccggcg cctccacctt gcagctgcgc
    16861 gaggaccccg acgacctccc gtggtgggca cgggtgcggg cccggctgct ggccgcgcgc
    16921 gccacccgcc cgcagccgag ccgggacgac aaggtggtga cggcctggaa cggcctggcc
    16981 atcgcggccc tggccgatgc tggtgtcctg ctcgaccgtc cggacctcgt cacggcggcc
    17041 gaggttgccg ccgcccacgt cctcgacacc cacgacgtcc cggcatccga cagtgcggac
    17101 gacggcgacc ggatgctgcg gcgcacctcg cgcgccggcc gtaccggccc ggccccggca
    17161 gtcctggacg accacgccaa cctcgccgag ggcctgctcg ccctccacca ggcgacggga
    17221 gagccacgct ggggcgaggc ggcggcatcc ctgctcgacc gtgcccttga tcgcttcgtc
    17281 gacgcggacg gcaccgtgca cgacaccgcc cacgacgcgc ccgccctgtt ctcccggccg
    17341 gcgaaccgct ccgacaacgc cgaaccgagc ggggcgtcag ccctggccgg cgcgctgctc
    17401 acccatggcg cgctcaccgg gtccgccgcg cacctgcagg cgtcgcgggc cgcgctcgac
    17461 gcctgcggcc cggtcgccgc gcaggacccg cgcttcgccg ggtgggcgct ggcggtcgcc
    17521 gaggcgagcg ctgccggtcc gctccaggtc gcggtcgtcg gcgagggccc cgcggcgcag
    17581 gcgctgctgc ggacggcgcg cgccagcacc tcccccggcc tcgtcgtcat gcacgggatg
    17641 ccggacgccc ccggccgcgc gctcctcgcc gaccggcccc tcgtcgacga cggcccggcc
    17701 gcctacgtgt gtcggggctt cgtctgcgat cgcccggtca ccgataccga ggccctcggc
    17761 gcggcgctca gatcacgatg agcccgtagg cggggtcgcg ctgctcgatg agggcgcggg
    17821 cccgctccgg gtcgacccag gcggcggtcg cccacacgtc ggcccacatc aggtcggggc
    17881 cgaccacggt ggccgacccg gggcgcacga tgccctgacc ggtccgcggg tcgacgacgt
    17941 gcccgccacg gatggcggcg cccgacgtgg cgaccgcacc acgggtcacc gtgaccactc
    18001 gcgcgagccc gccccgctgc cgggggtcct cgatgccgat gcgccacggt ggggcctcgt
    18061 cgaccccggt gcccgtcccg accacgacgt ctcccccggc cccgatgctg taggcgatct
    18121 cggggacgat ccgcaggtgc tcggcggccg cggccaccgc ccagcccttg accaggcccg
    18181 tggggtcgaa gctgagccgt ccaccgggcc gccgacgcca ggcgcgaaag agcccgtcgg
    18241 tgcggtcctc ggcctcgaga cagaggtcgg tgacgtcggc gagccacggg tgcacctcgt
    18301 cgaccccgtc gacctcaccg tgctgaacgc gcaacaggtc gctgtcggca cgccacgtgc
    18361 tgagcaccgc gtcgacccgg tggaggtggg cgaagacccg cgcggcggca gcctcgacgt
    18421 cgctgcgcga ggtgtcgacc gcgcgaacgt gcacgctcac cggcatcccc atgacctggt
    18481 cgacgaacgc gcggcgcgcg agcaccgggg aacacggcgc ggcgaccgtg gtggtcagcg
    18541 ccgtggtcac aggttcgccc ggtcgatggc cgactgcagc gaccggatgt acgcctcgga
    18601 ggtgtacgtc gcccccgaga ccatgtcgat gccggcactc tgcgcgtcga cgacctcggc
    18661 gttgagcacc ggcacggcgc ggccgttgat ctgctggtcc ttgccgttgc tccagggcac
    18721 ctgggtgacc tcggatgccg tgatcgcccc gttggcgacg gtgatctgga cctggacgtt
    18781 gccgtacctg gtcatgatcg cgtcgccgag gatcgtgcgg gccgcggtgc tcgtcgagga
    18841 tccgctcgcc gctgagctct cggtctgcgc aggggtctgc gtggaggtgt caggctgcgt
    18901 gggggtgtcg gtgccatcgg atcccgtgtc tgcctcggcc accacgctcg ccgccaccga
    18961 ggcggaggca ctggtcgacg tgctgtagct gaacagcagg acgagggtcg agatcgtgct
    19021 gagcagccag agggtgatgc ggcgcatggg tgcgtccctt ctcgggtcgg gtggatcagg
    19081 aggtgaagcg ctcgaggtgc acgcgcccgg cgggaactcc ggcggccagg gccgcggccc
    19141 gcgcagccgt catccagccg tcggcaccgc acaggtacac gtcgtggtca gcgatgtcgg
    19201 gcacgatctc gcgcagggcc tggtcatcgc tgaggtctcg ggcctgctcc ggtagccagc
    19261 tcgcgcggct ggtgatccgg gggccttcga tcaggaggta gcgggcaccc cgcgcggcgg
    19321 ccaggtcagc cagttcgtcg gcgagcacgg cctcgctgcg actgcgcacc cggtgcacga
    19381 cggtgacgtc acccgggccc tggggcaggt cctcgaggat ggcgcgcagc ggcgtgatgc
    19441 cgatgccact gcccatgagc aggaccttgc gctgggtgcg gaccccgtcg tgcaggcgcc
    19501 cgtagggccc ctcgaccatg accctcgtgc ccggacgaag ggtggccagg cgacgggagc
    19561 cgtcaccgac gtgggctgcg gtgaagcgca gggtggcgcc gtccggtgcc gcggagatcg
    19621 agtaggggtt ggcccgcgac cagcccggcc cgtcgaggaa gcgccactgg aagaactgcc
    19681 cggcgcgcac cggcatccga cggaccgagc ggccggcgac ggtcacggtg gtggcatccg
    19741 gaccctcggc gcgaacgtcc acgacgcgca acgacccggc gaatgagcgc cagagcggca
    19801 gcagcacccg gaagatgatc acggcgccgg cggtgctggc ccacagcccc caccagaaga
    19861 ccgtggcgag cgtcgaggag aggaactccg cgcccgtcca cagctggtgc ggcagggcca
    19921 gccccgcccc gaggtaggcg tagaggtgga tcaggtgcca ggactcgtag cgcagtcgcg
    19981 cccgggcctt cttcaccgac gtgaccacga ccatgacgag cgcgagcgtc ccggcgaccg
    20041 ccagcagcat ccccggatag gtgacgacga agtcgacgag ggtgccccac acgccagcgt
    20101 cggtcgaggc ggcgtacccg agggtgatga gcgcgatgtg ggcgagcatg agggtgaacg
    20161 aggtgaaccc gacgaggcgg tggacacggg tcagctcgtc ctggccccag gcctgctcga
    20221 cccacgggat gcgggccatc agccccacct ggagcagcag gagcgccgag gcgacgagac
    20281 cggtgagccg acccgtcgag gtgaggaacc ctgcggtggt gccgagttcc tggatgccgc
    20341 cgccgcccac ccacagggcg atggtgaaga gcaccacgcc ccagccgagc gcggcgctga
    20401 ggtcgcgcca ccggcgaggc accggcccga ggcggcggcg ccgcgtcggt cccgtgggac
    20461 gcgagaggtg tgacgcgcca cgaacgtgcg cggacggcat ccgggaggtg gtgacggtgc
    20521 tcgagctcat gcgaccaccg tcacggcgca acctgtgcgt cccctgtggt tggtctgggc
    20581 ctgccctgtc gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtccggc
    20701 actgtggcgg tctgggcaac caccatgccg gacgcacgca gacgtctgcc ccgccatcgg
    20761 caagaatgcc cgggtgagcg ctgccgtgcc cccgcccctg gccgcgacgt ctgccgcgtc
    20821 ccctccccca acctcccttc ctgccggggt ctcacccgac cgggaggtga tgttccgcgc
    20881 ccgcgtcgcc cgctggtggc ccgacctggt caacggcctg cggatggcct acggcgacga
    20941 gcgggccggg gcgctcggtg ccgacctggt cgagctcgcg ggggctgcct tcgccgcccg
    21001 ctcggaccgc ctgcacgtgc gtgacctcga gcgcatgctg cgccccgact ggctccagga
    21061 cccgtcgatg gtcggctacg cggcgtacac cgaacgcttc gccggtgacc tgcgtggagt
    21121 ggccgaccac ctgccctacc tcgacgagct cggggtgcgc tacctgcacc tcatgccgct
    21181 gctgcgcccg cgcgagggcg acagcgacgg cgggtatgcc gtggccgact accggtcggt
    21241 gcgcgccgac ctgggctcga tggacgacct cgagcacctc gcggcccggc tgcacgacgc
    21301 cgggatgagc ctggtgctcg acctcgtgct caaccacgtc gcccgtgagc acgagtgggc
    21361 cgtcaaggcg cgcgccggcg acccgacgta ccgcgagtac ttccacgtct tccccgaccg
    21421 cgacatgccc gacgcgtacg aggcgaccct gcccgaggtc ttccccgact tcgcccccgg
    21481 caacttcacc tgggacgagg acctgcaggg ctgggtgtgg acgacgttca acgagttcca
    21541 gtgggacgtc aactggagca accccgcggt gctgcgcgag tacgcgtcga tcatcctcga
    21601 cctggccaac cgcggcgtcg acgtgctgcg cctggatgcc atcgccttcc tgtggaagcg
    21661 cctcggcacc gactgccagg gcgagcccga ggtgcacgcc ctcacccagg tgctgcgggc
    21721 cgtgacccgg atcgcgtgcc ccgcggtcgc gttcaaggcc gaggcgatcg tggccccgac
    21781 caagctgctg ccctacctcg gcaccggcgc ctacagcggc aaggtcagcg acctcgccta
    21841 ccacaacagc ctcatggtgc acgtctggtc gatgctcgcc tcgcaggacg tgcggctggc
    21901 ggcgcacgcg ctcggctcgc tgccgcccaa gcccacgacg gcgacgtggc tgacctacct
    21961 gcgctgccac gacgacatcg gctgggcgat catggacgag gatgccggtg ccgtcggcct
    22021 gagcgggcac gcgcaccgaa gcttcctctg gcactggtac gccggcgacc acttcggctc
    22081 accggctcgg gggctggtct tccagtacaa ccccgagacc gacgacgggc gcacgagtgg
    22141 caccgcggca tccctcatcg gcctccagtc ggccggttct gcggctgagg tcgagtccgc
    22201 ggcggcggcc ctgcgcctgg ccaatgcgat ggtgctcggc tggggcggca tcccggtgat
    22261 ctggagcggt gacgagctcg ggcagccgaa cgacccgcgc tgggcgctcg agcccgggca
    22321 cggtgacgac aaccggtggg cgcaccggcc gcggctggac tgggagcgtg ccgagcaccg
    22381 ccacgaccgg acgaccacgg cggggcgcgt gttcggcgac ctcgtcgagc tcgtgcgggc
    22441 ccgcggctcg ttggtgcacc tgcacgggtc ggtcgagacg cagatcgcgc cggtcgatga
    22501 ccccggtgtg ctggtgaccg tgcgtgagca cccggtcggg cggttcgtcg gggtctacaa
    22561 cgtgacgccg cagtggcgga tgtggccggg gtggcgggtc aacgagctcg gtgtggcgga
    22621 tgccgtcgac cggatcaccg gggacgcctt gccgtggggt ggcgacggca acgtgtggct
    22681 gccgcccttt gccgtcctct ggctgaccac ctgaccctgc cacctgaccc ctgacccctc
    22741 gggtgcggct tctcgcgaac gtgggtgaag gtcgtcggct ggacgacgat cttcacccac
    22801 attcgcagga gagtcaccag gcgccgagga ggtctccggc gatgcggatg atgaaggccc
    22861 cgacgacgat gatgaacacg acgcgcacga agccgctacc cttcgccacc gcggttcggg
    22921 ccccgacgta cccgccgagc aggttggcca cggccatcac gccaccgacc acccacatga
    22981 cgtggccgcc cgggatgaag acggtcaggg ccccgaggtt ggtcgcgaag ttggcgatct
    23041 tggccttggc gctggcctcg aggaaggcgt agcccatgag cccgacgagg gcgaacacca
    23101 tgaacgagcc agtccccggg ccgagcgcgc cgtcgtacac gccgatgacg aagccggtga
    23161 gcatcgcgaa gacggtgtgc cgacgcccac tgaagcgcag cgccgtcgac tctcccagcg
    23221 acggcttgaa cagggtgtag gcgccgaccg cgatgaggag cacgaggatg atcgggtaga
    23281 acgccgcctt ggggatgtgc aacccgatga gggctccgac caccgcgccg acataggcca
    23341 cgccggccat cggcagggcg gtgcgaaggt cgggcttgac ccggcggtag tacgtcaccg
    23401 acgacgtggc ggtgccccag atcgagccga acttgttcgt cgcgaggatc tgcgccggcg
    23461 tggccccggg caccccgagc agcagcgcgg gaagctgcac gagcccaccc ccaccgacga
    23521 ccgcgtcgat ccacccggcc agaaagcccg cgaggccgag cagcgcgatg acctcgaccc
    23581 ccgggtcgct cacgggcggg tcacgcggcc atccaggcgt cgaccagggc gagcacgtcg
    23641 agcagttcgt gggcccggcc gtcgggcacg gccgtgacct cgacccgctg gttggcgggg
    23701 atgtcggagt gcgggatcca gatcgctcgc atgccgacct gctgcgggcc gtgcacgtcc
    23761 tcgaagatgc gatcccccac gtagaccgac cgtcgcgggt cgacgtcgag ctcgcggcag
    23821 atcgcccgga acgcctccgg gtgcggcttc acgtggtcga gctcgctgga gtacaggtct
    23881 gcgtcgacga ggtcgagcac cccgtcacgc tcgaagatgc cctggtggta ctcgcgcgac
    23941 cagatggtgt tcgagagcac ccccaccttg atcccccgag cgcgcagccc ctcccacagc
    24001 ggccgcacct gcgggtcggt gtgggtgtgc ggctcccaga agccgcggta ggccgcgagc
    24061 gccaggtggt gccggtcgtg cgcggcatcg atcccagcag cctcgaggat gtctgccagc
    24121 gcggccgacg tgtgctcgtc gcgacccctc gtccaggcag cgctctcgac ctcgaggatg
    24181 cggtcggcga gccggtgcgc ctcgtcaaga tcggcctgcg agacgtccgg ggagtcaacg
    24241 gggatgccgt ggacctcccg cgcgaagacc cgccactgct cgggcaggtc aacctcgtgc
    24301 cacggggtca gcgtgccgcc ccagtcgaag acgaccgcgt cgaccgggtg gctcatgcct
    24361 gcccgatctc cgcgaggatc tcctgcaccg ccgcgtcgac cgcgacctcg cgccgctccc
    24421 cggtgcgccg gtccttgacc tcgatgacgc cctgcgccag cccacgcccg atgacgacga
    24481 tggtcgggac cccgatgagc tcggcatcct tgaacttcac acccgggctg gcctggcggc
    24541 ggtcgtcgag cagcaccgcc acaccgcgcg cctcgagcgc acgagtgagc tcttcgccgt
    24601 aggtcgcgac ggtctcgtcc ttgccggtga cgacgacgtg cacatcggcc ggggcgagag
    24661 cgcggggcca gatgaggccg agctcgtcgt ggttgccctc ggcgacacag gccacggccc
    24721 gcgagacacc gacgccgtac gagcccatcg tcacggtgcg cagcttgccg ttctcgtcga
    24781 gcaccttgag gtcgagcgcc tcggcgtact tcgtgccgag ctggaagatg tgccccatct
    24841 cgatgccgcg ggcgctctcg agcccgtgac cgcacgacgg gcaggcgtcg ccgtcgcgca
    24901 cctcggccgc ctcgatggtg ccgtcgccct cgaagtcacg gcccttcacg aggtcgaaga
    24961 cgtgcttgcc agagatgtcg gcgcccgtga tccacgcggt gccgtcggcg acgcgggggt
    25021 cgagcaggta ccggatgccg gtcgacttct ccagacccag cgcgccgggg ccgatgtagc
    25081 ccttggccag cgtcgggtag ttcgcgaagt ccttctcctc gaacgcctcg acgcgggcgg
    25141 gctcgacctg ggcaccgagg cgcttctcgt cgacctcgcg gtcgccgggc acgccgatgg
    25201 ccagcggctc gcgggtgccg tcgggcagca cgagcatgac gacgacgttc ttcagcgtgt
    25261 cggcagcggt ccaggcccgc ccatccgccc gggggtggtc ggcgttggcg gcagcgacca
    25321 acgtgtcgat ggtcggggtg tcgggggtgt cctcgacgtg cgcggcgggc agggaggtga
    25381 cgtccacggc atccggcgtc gggacgcgca cggcctcgac gttcgcggcg tacccgcagt
    25441 tcacgcaccg cacgtaggtg tcctcgccgt tctcggcggt ggcgaggaac tcctcggact
    25501 tcgagccgcc catggccccg gccatcgcct ggacgatgac gtagtggaag ccgagccggt
    25561 cgaagatgcg cacgtaggcg tcgcggtgca gctggtagct cttgtcgagc ccggcgccgt
    25621 cgatgtcgaa ggagtaggag tccttcatga cgaactcgcg gccacgcagg atgccggcgc
    25681 gcgggcgggc ctcgtcgcgg tacttggtct ggatctggta gatcgacagg ggcaggtcct
    25741 tgtacgagct gtacaggtcc ttgacggtga gggtgaacat ctcctcgtgg gtggggccga
    25801 gcaggtggtc ggcccccttg cggtccacga gccggaagac gttctcgccg tactcggtcc
    25861 agcgcccact cgcctcgtag ggctcacgcg gcagcagcgc cgggaagagc agctcctggc
    25921 tgccgatggc gtccatctcc tcgcgcacga tcgtctcgac cttgcgcagc accttcaggc
    25981 cgagcggcag ccaggtgtag acgccggggc tgacgcgccg gatgtagccg gcccgcacga
    26041 gcagctggtg gctcgggacc tcggcgtccg ccgggttctc gcgaagggtt cgcaggaaca
    26101 gggacgacat gcgcatggcc acgggagggc tcctcggtgc ggtcgggtcg gtcgggcaac
    26161 ctgccaagga taacgacgtc agcggatgcc gtgcgcgtcg cctcggttga cgcgcacctc
    26221 cgcgccccac ccccggtcga gagtaggcgg atcgccgtct cgccgcggat gcagacggcg
    26281 attcgcctac tctcgactgg aggggtcagg cggtgagggc gctctggccg aggaccagaa
    26341 cccccatgac gaggacgaac cagccgaagc cctgacgcag ccggtcctgc ggcacccggc
    26401 ccgacagcag cgcaccgccg atgctgccga gcacggccat cgcggtcacg gcggccgcga
    26461 gcccccagtc gatcgacacc gaagacaggt agccggcgag gcccgcgaac gacttcatgg
    26521 cgatgacgag cagtgaggtg cccacggcca ccggcatggg cagcccaccg aggagcacga
    26581 gggcggggac cacgaggaac ccaccgccag cgccgacgag accggtgacg aggccgacca
    26641 ccactccctc gagcagcacg agcttgacgg gcagctcgcg caccgaggcg ctgccgccag
    26701 cgtcaccggc agccgacccc gagcccggcc cggcggcatc ctgcctggcc cggcccctga
    26761 tcatggcgac cgccgtcgcg accatcatga gcgcgaaggc cacgagcagg accgtgccgg
    26821 ggatgaactc ggcgatcctt ccacccgcgt aggcccctgc catcgccgcg gcaccgaaga
    26881 tcgcgccggt ccgccagcgc acccgtcctg cgcgggcgtg cggcacgaga gccgcgaggc
    26941 tggtcacccc cacgacgagc agcgacgtcg cgatcgcctc cttcgcgtcg agcccagcga
    27001 tgtacaccag caggggcacg gtcaggatcg agccgccacc gccgagcagg cccagggcca
    27061 tcccgacgag cacggcgagc agcaacacca cgggagacat gagcccagca taccccctag
    27121 ggtatgtcgc tcgggacgtc cttgccctgg atgggcccca cctgctggta gcgcgcccgc
    27181 acacccgagt ccaggaagcc cagcgagcgg tacagcgcgc gggcactcgg gttgtcgacg
    27241 ttcaccgcga gccaggcctg ctcgtagccg gccttgcgca acgtccgcag cgaccggacg
    27301 acgaggtgcg caccgagccg ctggccccgc cactcgggca ccaccccgac ctgatcgatc
    27361 cagttgtcgg agatcgtcac gaaccccgcc acgtgtccgt cctcgtcgag cgccacccgc
    27421 gagaggtcgg ggtggaaacc ctcgtcctcc tcgaccgacc tgacccactc agcgaacggg
    27481 gtgtcaggga agcccgggcg gttcgcgaac gaccgcgagt aggcggcgtg gaagaggtac
    27541 tgggtgtccg cggtccacgg gtaggtgtgc acctccgcgg gccgcacgat gcgcgggacc
    27601 tcgacgagcg ggcgacgcat cacgtgctcg gcgaacgtgc gctcgaggcc gaggcgtgcc
    27661 gcgaaggcgt cggactccgg tgacgcggtc tcggcgacga cgcgcatcgg ccctcccccg
    27721 gcctgggcga gccaccactt cagcagctgc tcgccgatcc cctgcgagcg caccgacggg
    27781 tggaccagcg ccgtgaccga gcgggtcgcc gacgcgtcga ggaaggctcc ggccgcggcg
    27841 atcagctcac ccgtctcatc gcgcccgccg acagagtgcc cggagaggaa gagctgtcgc
    27901 agcatcggct cggtcgccag cagcggcagc ccaccgtcac gctcgaggca gcactgggcc
    27961 aagtgggtca gggcaggaag gtcaccctcg gtgagaggct tccagtccag ggtcgacatg
    28021 ccgggctcag cggaccgggg cggcgacggg gcggatgcgg gctgcgtgag ccatacccca
    28081 tccaaccact accggcacgg gcccctacag gagcaccgtc gcgaagtcgc ccgcgtcacg
    28141 gaacccgagc cgtcgatacg tcgcccgtgc ggccgtgttg aagtcgttga cgtagagcgt
    28201 gaccagtggc gccggaccga gcatgacctg ctcgacgaca ctcgcgagca tcggcgccgc
    28261 caagccctgg ccgcgcaggt gcggcgcgag ccacacgccc tggagctggg cgcagccgag
    28321 cgcgaggctg ccgatgtccg tcttgaagac cacctcaccg tgctcgatga cgacgtaggt
    28381 gtggccgcgc tgggccaggg tcgccagcga ggccaggtag ccccgggagc tcccccggta
    28441 gggcgggtag ccgatctcgg cggtgaacat gtgctcggcc gccggcagga cgaggtcgac
    28501 ctcgtccacg cgcgccgggc gcacccgctg gtcgatctgg acccccagcg tcgacggcag
    28561 tcggtcggcg acgagcaggg gctggcgggt gcggatcgct cgcgcgggcc cccagtgggg
    28621 cgcaaggtgc gaccagagcc cctcgacctc gtcgcggcgc ccgaacagtg acgcgcagcg
    28681 gcccttccag cgacggaccc tctcggcgat cagggcctgg ctctcgggtg tggtggccac
    28741 cgggacgacg ttggcgctgg cccagcacag cgaggtgagc tcaccgtcga cgcggtgtcc
    28801 gagcacgctc atcagcgacc cggtgcgggc cccctcgagg atgcgcgccg ccacgaacac
    28861 actgcccggg aggtcgcgag aacacaggtc cagggcgtcg tcgcggtcag cgagcgagag
    28921 gggcctgatc ggggagcgcg tgcgcagcac cctgtcagcg taggcgcgac gtggcctcg
//
LOCUS       DAOSOC010000011        28179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1687_length_28179_cov_9.148876, whole genome shotgun sequence.
ACCESSION   DAOSOC010000011 DAOSOC010000000
VERSION     DAOSOC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 28179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28179
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1687_length_28179_cov_9.148876"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PL014_01850"
     CDS             complement(<1..1006)
                     /locus_tag="PL014_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT55866.1"
                     /translation="MSPFEYAPAPESAAVVDIASSYGLFIGGQFVPATGGTPFKTVNP
                     ATEEVLAEISEASSDDVDAAVAAARTAYTRVWSRMSGADRGKYLFRIARILQERAREF
                     AVLETLDNGKPIKESRDVDLPLAAAHFFYHAGWADKLDYASLGPNPRPVGVVGQIVPW
                     NFPLLMLAWKVAPALACGNTVVLKPAETTPLTALLFAEVCQQADLPPGVVNIITGAGP
                     TGQAIVDHPGIDKLAFTGSTAVGKMIARSIAGTRKRATLELGGKAANIIFDDAPIDQA
                     VEGIVNGIFFNQGHVCCAGSRILVQENIAEDLDRRLKRRLATLRVGDPMDKNTDVGAI
                     N"
     gene            complement(1038..2027)
                     /gene="deoC"
                     /locus_tag="PL014_01855"
     CDS             complement(1038..2027)
                     /gene="deoC"
                     /locus_tag="PL014_01855"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT55867.1"
                     /translation="MSTATDVDPTARARDLLGVSRLTNQALTSWLHGLPGVDQVGCEA
                     RAAALGTRSIKTTSKAWAIDTAISMIDLTTLEGADTPGKVRGLAAKALVPDPTDMSTP
                     RPAALCVYGDMVAVAKDALGGAAINVAAVATAFPSGRASMPVKLADTRDAVEAGADEI
                     DMVIDRGAFLSGDYLTVFSEIAQVKEVCGSARLKVIMETGELVTYDNVRRASWLSMLA
                     GGDFIKTSTGKISPAATLPVTLVMLEAVRDWHDTTGEMVGVKPAGGIRTSKDAIKFLV
                     AVSEVAGEAWLTNEWFRFGASSLLNDLILQRQRMRTGAYSGGDYVADDSPSLY"
     gene            complement(2141..3826)
                     /locus_tag="PL014_01860"
     CDS             complement(2141..3826)
                     /locus_tag="PL014_01860"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT55868.1"
                     /translation="MSAHLTDLLAAARAWAADDPDAVTRDELVGLADAAEGGDEGAAT
                     ELADAMAGMLEFGTAGLRGKLGGGNNRMNRAVVIRAAAGLTAYLKTLTPEPFVVVGFD
                     ARTNSDVFARDTAAVVVAAGGRAAILNRTMPTPVLAYAIRWLGADAGVMVTASHNPPQ
                     DNGYKVYLGDGSQIVSPVDALIAEQIAQVSSVACVARADDGWETLGDDVWQDYLRDAA
                     AVVEHGAAAELSVVHTALHGVGTETVLAAFERAGFSAPNVVESQAQPDPAFPTVSFPN
                     PEEPGAIDAAIELAEQTGPDVVIANDPDADRCAAAVPGPGGWRMLRGDEVGALLGAHL
                     LAKGSAAGVLPDSAECVFANSIVSSRLLAAMCRAAGVRHEETLTGFKWISRVPGLRYG
                     YEEALGYCVDPAQVRDKDGVSAALLFAELTAGLKAGGRSVDDLLDDLARAHGVHATDA
                     FSVRVEDLSLIGEIMARLRATPLTSVAGVDVARADDLAQGDGGLPPTEGLRYYLADES
                     RIIVRPSGTEPKLKVYLEVIEPVGTDEEVKTARGRAGSRLAGIRADLEAATRL"
     gene            complement(3834..4673)
                     /locus_tag="PL014_01865"
     CDS             complement(3834..4673)
                     /locus_tag="PL014_01865"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT55869.1"
                     /translation="MTDNAAAHALSDPATDPFAVADAAAAVIRERTGVAAHDVALVLG
                     SGWGQTGDLIGETLATIDNTDVPGFAKAAVAGHSGVMRSVAIGDTGRRALVYGTRTHY
                     YEGHGVRAVVHGIRTAAAAGCRTVVITNGCGGLREEWAPGTPVLISDHINLTATSPIE
                     GANFVDLTDVYSPRLRAIAKEIDPDLDEGVYVQFRGPHYETPAEVRMAGILGGDLVGM
                     STSLEAIAARQSGLEVLGISLVTNPAAGISKTPLNHEEVLEAGREAAQRCGRLLAAVV
                     AKI"
     gene            4813..5391
                     /locus_tag="PL014_01870"
     CDS             4813..5391
                     /locus_tag="PL014_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOT55870.1"
                     /translation="MRRILPVAAVLALALTTAACGSDEAEPAASSASAAATTEAPSPS
                     PSETMAASDIVDTAVAAGDFTTLAAALTAAELVDTLKGTGPFTVFAPTDAAFEKLPAG
                     TVDTLLKDPKGDLTQILTYHVVPGKVMAADVVKLDGQKVATVQGGELTVNVDGDKVSL
                     TDVAGNTVNVTATDVEASNGVIHVIDAVLMPK"
     gene            complement(5560..6609)
                     /locus_tag="PL014_01875"
     CDS             complement(5560..6609)
                     /locus_tag="PL014_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55871.1"
                     /translation="MTDSPRGTPVQLTELRRSYGGVAALDGLTLHMEPGEFVALLGPS
                     GCGKTTALRCLAGLEDPDSGTIVVGGNDLTKVPTNKRDMGMVFQAYSLFPHMTARQNV
                     EFGLELRGRDKAARRARAAEMLDLVGLVEHADKFAHQMSGGQQQRVALARALAIEPQV
                     LLLDEPLSALDAKVRTQLRDEIRRIQLEVGTTTLFVTHDQEEALAVADRVGVMSVGKL
                     EQLAPPAELYSAPKTPFVADFVGLTNRIRGEVAGETAHLLGGSVPLLPGSSSGPSALV
                     LVRPEGVRIAHDDGGTASVVSASFLGGHGRVIARIPDGTLVTVQVPNTDVTTFRPGDR
                     VTLSPRGDAALAVAD"
     gene            complement(6606..7469)
                     /locus_tag="PL014_01880"
     CDS             complement(6606..7469)
                     /locus_tag="PL014_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT55872.1"
                     /translation="MSGQRATLRRRQTTFRWVAVLLATFLFALPLLGMLDFTTQGPGG
                     VGRTADTWRTLVDVSTITADYPALRQGFLASIGLAIVTPLVMLAILVPTMTWVRLRLP
                     GLSRTVEFICLLPLTVPAIVLVVGLTPVYAWVYYFVGSSTLWLSLAYVVLVLPYAYRS
                     LDAGLRAIDVRTLSEAARSLGASWFTVMRRIVLPNLRSAVLSASFLSVALVLGEFTIA
                     SLFSKKNLQVAMYELGKSDAKISVAVGLVSLVFAFVVLFAMSFVGSSPAGTSPLARFR
                     RTRVDRKEPAA"
     gene            complement(7466..8293)
                     /locus_tag="PL014_01885"
     CDS             complement(7466..8293)
                     /locus_tag="PL014_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT55873.1"
                     /translation="MGVIPFFAYTTVFLLIPTLVVIVGAFLDADGSPTTANIEALTSP
                     SVVRGLRNSVVLSAVTAVVGAIVGGILAYAVSTASATSVVKRFITSLCGVLAQFGGVA
                     LAFAFVASFGATGLITAWLAAIGVDTGTGFWLYEWNKGLMLIYLYFQIPLMVIVFLPA
                     VEGLRPQWREATETLGGTTWTYWRRVAGPILMPSFVGGMLLLFTNAFSAYATAKALIS
                     QGSPLLPLQIAGTMSSEVILGQENVGKAMALAMVVVVALVMTAYVMLDRRTSKWMPR"
     gene            complement(8343..9488)
                     /locus_tag="PL014_01890"
     CDS             complement(8343..9488)
                     /locus_tag="PL014_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT55874.1"
                     /translation="MTHPFTRRAGALAVIGFAAAAVVACSPPADEETPAPAGSSGSVD
                     AKTATSAEAFGGMEGLVAAAKAEGKLNVIALPPDWANYGEIIKGFQAKYPEIKITSDQ
                     PDASSADEIATAERLKGQAGAPDVFDLGAAVALANTDKYAAYKVATWDDIPADLKDAN
                     GLWVNDYGGYMSIGYDSAKVPAPTSVADLLKADYKGKVALNGNPTEAGAAFAGVQMVS
                     IAQGGTAADLTKGVDFFKQLTQAGNFLPVDPTPATVESGQTPLVIDWDYLNAGLSAKL
                     PTWKVMVPKEAVVAGFYYQAINADAPNPAAARLWQEYLYSDEGQNLWLKGGARPVRFE
                     AMKTAGTLDAAAAAALPPIEGAAVVPTQADAEAGKAYLAENWANAVG"
     gene            9678..11081
                     /locus_tag="PL014_01895"
     CDS             9678..11081
                     /locus_tag="PL014_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HOT55875.1"
                     /translation="MSNRSSKVVILGGGPGGYEAALVAAQLGADVTIVERDGIGGAAV
                     LTDCVPSKALISTADYMSDFETAHGLGVNLEDAEGDEVSDAVADLFEVNRRVRALAAA
                     QSADISTRLAEVGVRHVAGTASLTSPTSVRVEGPEGTEDLAADVVLVATGATPRVMDT
                     ARPDGERILTWQQIYDLDALPERLVVVGSGVTGAELAQAYLGLGSQVVLVSSRERVLP
                     GEDPDAATVIEDVFRRRGMEVLSRSRMAAVDRTDAGVRVTLEDGRVVEGSHALLAVGS
                     TPLTRGIGLEEVGVELTPSGHVVVDKVSRTSVRGVYAAGDCTGVLPLASVAAAQGRTA
                     MAHALGDAVAPLPLHYVSANIFTDPEIATVGVSHADVESGRVRADVITLPLARNPRAK
                     MLGITDGFVKLFAHRGSGLVLGGVVVAPRASELIFPVTLAVQHRLNVDQVASTFTVYP
                     SLSGSIAEAARRLHVTY"
     gene            11199..11450
                     /locus_tag="PL014_01900"
     CDS             11199..11450
                     /locus_tag="PL014_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55876.1"
                     /translation="MARRAIKLAALITVAQQAREYAQKNPAQVNAALDRFEVTVRSKL
                     HPKYSAHVGKGSHALRSGLGIASAPPRAGSVQDTPPPQY"
     gene            11680..>14767
                     /locus_tag="PL014_01905"
     CDS             11680..>14767
                     /locus_tag="PL014_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT55877.1"
                     /translation="MRSFVTRVLAIVALVVGSTLPVLPAQAAPDSLVPAPTELTFACA
                     LKSNGQMRWVQSLSSCSKKDTRVTVKPGPTRVCIQPSGSTRYVTSFSGCRPPATALTL
                     PPTSGTVYFCAAAGSGVLRFVTDPSLCLAGETPLQATPNDAAPTLSSSLPATGSTSVA
                     TTAVVQLTFSEAVTAPAGAFAFTCGVTPVAFALSGAPGAVLTLTPMTPLQEGTSCSVT
                     VLGGSVSDVDAIDPPDVMATNPVVTFTTDAAPTLVSSTPADGATDVAVGSDVVLTFSE
                     PVAVPAAALDLVCAASPVGFTVEDSGTSTITVNPAAALPASAGCLLTVTGSAVSDVDG
                     GDPPDAMAGTATVGFTTADEAPTVVSTTPADGATGVGTSAGLSVTFSEPVTATSDAFT
                     LTCGGTPVAVTADAGPATTFAVTPGGPLPTGVTCTFTVVGAEVADVDTVDPPNVVDGN
                     TAVTFETATNNAPTAVDLTPRVVAENSPSGTTVGALSTTDADAGDTFTYTLVPGAGST
                     DNALFAVVGTSLQTAAGLDAEDGSRSVRVRSTDAAGDWVEAAFTITVTDVNEAPTDVT
                     LTHSSVDENEPAGTAVGTLGGSDPDAGQTLTFALVTNGCGSTYPGSASFAVDGTTLET
                     AASLNHEVTPTIKVCVRATDSASPGLSLDEAFTITVADVNDAPTTAPDEYLGAVGNTM
                     FALNLPGTPAPRVAVTGAVLTANDLDEDGDTLRTVAETVASTGGGTATIDAAGNFTFL
                     PGVGDKGQTDSFAYTVTDGAALATGTVTVTIGATVVWWVDNASSSASPDGRSTSPLTS
                     TQALNGPDATDVDSAGDHIFVFTGTATYQGGLRLEPNQTVLSSRTGITVGPDALVAPG
                     ATSPVLTNAAGNGIDLASGTSVSGIDVSGASGDGVNGAGISTTSVGSEVPMTISGSVG
                     DGVDLSGAGSGAVTIGATITTSGARSLVVSGRTGSTVTVSGAVTGKGVELSSNTGATI
                     SLTGALALTTTTTPAFSATGGGTVSTSGSPNTASAGTGGAVIVENTTIGAPGLTFRS"
     assembly_gap    14768..14867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14868..16361
                     /locus_tag="PL014_01910"
     CDS             <14868..16361
                     /locus_tag="PL014_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55878.1"
                     /translation="AVIVENTTIGAPGLTFRSVSANGAVNGIRLVNTGATAGLTVTGG
                     GSVAQGGDVSGGTITATSGPGILLTNTRNPSFNNVSVTNVGTAAGVKGTGVTGFGFSN
                     GRVDGSGLGGADNASANLAFNTVSAGVSNLSGVVSVTNSVLANAYGSGIDIYNESGTI
                     SSLTVSGNVVQSSTSTTTSKGHGVIVQSLGSAGGASAVTAAAIANNRVLNFPSGGGIL
                     LYGGNVAGASATILGANAGSKVVISGNVVRGASDGTPMNTNCIYVSVAGYGTGFVDVQ
                     NNGTVGEPLGANRGNCISVNSTGAATLTSLVSSNRVAPGAGQIGGAFGIAGGAAKQTV
                     VGPTDLNSAVLNLTVDGNAVSATKSSGISLLTRDSGTLRSRVQNNNVAAPVELAGESG
                     IVVTSGDQIAGDATVCLQILNNSTAGSVNSVAGGTAPGIGLFKRGTVQTTNDFGVSGL
                     TATPTSAADVVTYVSSVNSGSALGSGIYGTFRAQVSGNNYVPCTLPF"
     gene            16408..16914
                     /locus_tag="PL014_01915"
     CDS             16408..16914
                     /locus_tag="PL014_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HOT55879.1"
                     /translation="MSEPFLSEIRLMSFVFPPKGWALCNGQLLPINQNQALFALLGTT
                     YGGDGRVTFALPDLRGRVAAHVGDGHTLGEVGGQAAHTVTVSEMPSHSHMVVGTTATA
                     TTRLVGPTVGLAASTGSAAYSPPGSPVAMAANALGSAGSSQPHENRQPFLTLSFCIAL
                     QGIFPSPS"
     gene            16933..17457
                     /locus_tag="PL014_01920"
     CDS             16933..17457
                     /locus_tag="PL014_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HOT55880.1"
                     /translation="MGTPFVGEVRLFAGNFAPTGWMDCDGQLLPISEYETLFQLIGTT
                     YGGDGQSTFALPDLRGRAPVHQGTGPGLSTVVIGEDGGAESVTLTQAQVPMHTHALIG
                     SAANATATSPAGGVPGTMPEVAASAYGSDAPLTTLSPQGLAPAGGSQPHDNMQPYLTV
                     RYIISLFGIFPSQA"
     gene            17475..17999
                     /locus_tag="PL014_01925"
     CDS             17475..17999
                     /locus_tag="PL014_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HOT55881.1"
                     /translation="MTDPFVAEIRLFPFNFPPKGWAWCDGQIMPIAQNTALFSLLGTT
                     YGGDGRTTFALPNLQGRVPLHEGHGPGLSQRFLGESGGSDTVTLLESEMPAHAHALTA
                     SAAAGYARSPSGELFANQSGGVNSYAPPSGPQALLASTVTAEAGGDAPHTNMAPYLVV
                     YYCIALQGIFPQRP"
     gene            18001..18483
                     /locus_tag="PL014_01930"
     CDS             18001..18483
                     /locus_tag="PL014_01930"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT55882.1"
                     /translation="MPVTSRPALAADRPFLLALYVQTREDEFSAAGLAPAALALLLEQ
                     QYRAREAGWAHTAQGAEDRVVERDGRPVGRLVIDRRADGIRVVDIALVEDEQGRGTGT
                     ELLRGVLDEADAAHAPVKLHVVATSPARRLYERLGFEPVSADGAGEGVHVLLERRPSA
                     "
     gene            complement(18502..18882)
                     /locus_tag="PL014_01935"
     CDS             complement(18502..18882)
                     /locus_tag="PL014_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55883.1"
                     /translation="MSAPRRRAVITGAAAVAATGPMTTATASSAQAAKQQTDLLRRSR
                     FTPHVGSTFTSTSAAGRWSVVLASVADLGPGGVAGGEKQFAVSFTGTLPGDGVYTFAR
                     KGFTATPLFVVAARGRPLAVVNCL"
     gene            19146..19619
                     /locus_tag="PL014_01940"
     CDS             19146..19619
                     /locus_tag="PL014_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55884.1"
                     /translation="MDLAALLLESQSGIVEESYATLERSHVNHYEAAGEEFTRQRLDE
                     LFLLVVSAIRDRDLAQVSVFSRRVAEERFNQGFDIGEVQAAFNALEQTIWRHVVAVAP
                     AEDLAEATGLLSTVLGFGKDVLARTYVSLASRRRVPSLDLTALFAGTQPREVEPS"
     gene            complement(19901..21682)
                     /locus_tag="PL014_01945"
     CDS             complement(19901..21682)
                     /locus_tag="PL014_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOT55885.1"
                     /translation="MAISKVLIANRGEIAVRIARACADHGIASVAVYADPDRDALHVK
                     VADEAYALGGTTPGDSYLLQDKLIEVAHQAGADAVHPGYGFLAENAGFAQKVIDAGLV
                     WIGPGPEAIDSLGDKVKARHIALAANAPLVPGTPDPVEGPDEVVEFAKQHGLPVAIKA
                     AYGGGGRGLKVARNLEEIPELFESAVREAVTAFGRGECFVERFLDHPRHVETQCLADQ
                     HGNVVVVSTRDCSLQRRNQKLVEEAPAPFVTDEQHTELLRASKEILRRAGYVGAGTCE
                     FLVGPQGDISFLEVNTRLQVEHPVSEEVTGIDLVREQFRIADGEALTYPDPEPHAHSI
                     EFRINGEDAGRGFLPAPGTVTTMRVPQGPGVRWDSGLLEGDTVAGAFDSMIAKLIVTG
                     RTRTEALARARRALDELVIEGMPTVTPFHRAVVADPAFAPADPDEKFTIHTRWIETEF
                     NNTIEAYSGGSADAPEAQERQSIVVEVGGKRLEVSLPGGLSLGGGAGHGGPGARKKAP
                     KRSTGAKAGAAASGDSLTAPMQGTIVKIAVEEGATVAAGDLIVVLEAMKMEQPITAHR
                     AGVVTGLSAEVGATVSNGAVLCEIKDA"
     gene            21945..23219
                     /locus_tag="PL014_01950"
     CDS             21945..23219
                     /locus_tag="PL014_01950"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOT55886.1"
                     /translation="MTAPPTIPPDAPAGSPGQPGQPGPAPHAPVVAATPGQLHWLEGE
                     LAHWQSEGLVDPVAASAIRNRYVASRRFTLSHIVLTLGACFVGLGLIWLVASNLDQMS
                     PLVRFLLMVVIWLGLIGTAEVLAARREREGDTSSPVVGAVRLLAAGAFGAVVFQAAQS
                     LQVPAFEPILVGVWGLGAVLWAYAVRGVAPLVLGIGLIAFWFVWQVMSAGEGAFAVST
                     ALAAAALAGVSVGVVHSMVGWRDFALPWREIGAALGLVALFIAALPFAWGEAAGSIAL
                     WLGLAVAGGLAVLALARGDRVDRFEVALSALALALTIALSLWRFDQDVMDTANLPPGA
                     WARAVFAVVAYLFVASGYAVLGGMRDTSRLTWLATAALVIFVTVQAFAVFAPIVSGAA
                     LFLIVGLVLIGTGILADRGRRRLVSETKEALS"
     gene            23216..23791
                     /locus_tag="PL014_01955"
     CDS             23216..23791
                     /locus_tag="PL014_01955"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HOT55887.1"
                     /translation="MTALESGIRPDPAQPVPPTGRSRTGHLARVAGVVLTSLALLAVA
                     VWPQLSARFTGDEVVLRVQPVDPIDPFRGAYVDLGYPDLPRQQFDEVNPVTEEEQQAL
                     DAQRGIAYVPLRQQGEVWVGGEVVRTAPADGLYLTCDDSSWRLRCGIESWFLPQADAK
                     AFEDAVRDGRAVATVRVDGSGHAALVDVETR"
     assembly_gap    23821..23920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23935..24780
                     /gene="kynA"
                     /locus_tag="PL014_01960"
     CDS             23935..24780
                     /gene="kynA"
                     /locus_tag="PL014_01960"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HOT55888.1"
                     /translation="MDRAQRVLEEGISTDFSKAMSYGAYLDLDTLLAAQHPRSEPQQH
                     DELLFIIQHQVAELWLKLLLHEMHSARALIAADELAPALKRLARVKHVQKQLVEQWDV
                     LATLTPSEYAQIRPFLATSSGFQSHQYRAVEFLLGNKNADMVRLFEHSPHEHAELMRL
                     LGEPTIYDEFLRFLARRGYPVPSAVLERDVTQPYRLDEDLVAVFEAVYASPSEHWGVY
                     ETCEELVDIEDLFQQWRFRHLQVVTRTIGHRQGTGGSSGVGFLRKALDLTFFPELYAV
                     RALVE"
     gene            24853..25776
                     /locus_tag="PL014_01965"
     CDS             24853..25776
                     /locus_tag="PL014_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT55889.1"
                     /translation="MPRVIHTAQALVDAVMEVPSLPVRGGNVMATSYRRYAGGAVTIL
                     LAAARSGVECVHAGAVGTGPNGDLVRGALAAEGVTLSAPPVADLDTGVCVVLVEPSAE
                     RTFVTTQGAERQITVQSLAASNPVPGDLVCVSGYSLVGRTRDPLVEWLDTLADGVVVV
                     LDPGATFAGLAEPLRDRVLARTDVWTGNADEAAALTGVRGMREAAEVVATLLAPDAVT
                     IVRDGARGCAVRESGRTTHCPGHPQRPVDTNGAGDTHTGVLLAERATGASWPEAAARA
                     NTAGAIKVTRRGPDTAPTRAEIDAFLAALPR"
     gene            complement(25831..26319)
                     /locus_tag="PL014_01970"
     CDS             complement(25831..26319)
                     /locus_tag="PL014_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT55890.1"
                     /translation="MTSPTTDAVRSPTAGSGRESAAGRRRGRTETGEGTWSIAQIADE
                     FGVTHRTVRHYEDLGLISPERRGTTRVYHRRDRTRLGLILRGRRLGFPLDEIRTIIDL
                     YDVPRGRRSQLEYVLAQIGERRSDLEQRRRDLESALAELVDFERRCREDLEGLDEPGT
                     LR"
     gene            26420..27607
                     /locus_tag="PL014_01975"
     CDS             26420..27607
                     /locus_tag="PL014_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT55891.1"
                     /translation="MFELSKDHEDFRAVVRDFATEAVEPHVAQWDRDHHFPADLVPRM
                     GELGLFGLVVPEAYGGSLEDADGGAFTSLCIAIEELGRVDQSIGITLSAGVGLGINPI
                     LTYGTDAQKERFLPDLVAGRALAGFGLTEPDAGSDAGATRTKAVLENGEWVVDGSKAF
                     ITNSGTDITSVVTVTARTGTDADGRPEISAIMIPSGTSGFTVEAPYDKLGWNISDTHG
                     LSFAGCRVPEENLLGQRGHGFRQFLKTLDDGRIAISALALGLITRMREEATAYAKTRT
                     AFGRPIGVNQGVAFPIADLLVMEENSRLLTYRAAWLKDEQEAGRRSVADVKQAAAVAK
                     LYTSEAAVTATRIATQVFGGNGFMEEYPVARFYRDAKILEIGEGTSEVQRMVIAKSLG
                     LPS"
     gene            27604..>28179
                     /locus_tag="PL014_01980"
     CDS             27604..>28179
                     /locus_tag="PL014_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT55892.1"
                     /translation="MTQPGGGDPRVADVRARLEAAHTASERPTEKAAAKLASQNKLYV
                     RDRIALLIDEDSFVEDGRYANSRAPGLPADGVVTGRGTVDGRPVIVIANDPTVKAGSW
                     GARTVEKIVRATEAALREELPVFWLVDSAGARITDQVEMFPGRRGAGRIFHHQVALSG
                     KVPQICCLFGPSAAGGAYIPSFCDLIIMVEGN"
BASE COUNT         4007 a         9907 c        10020 g         4045 t
ORIGIN      
        1 tgttgatggc gccgacgtcg gtgttcttgt ccatcgggtc accgacgcgc agggtcgcca
       61 ggcgccgctt gagccgccgg tcgaggtcct cggcgatgtt ctcctgcacg aggatccggc
      121 tgcccgcgca gcacacgtgg ccctggttga agaagatgcc gttgacgatg ccctcgaccg
      181 cctggtcgat gggggcgtcg tcgaagatga tgttggccgc cttgcccccg agctcgaggg
      241 tggcgcgctt gcgggtgccg gcgatcgagc gggcgatcat cttgccgacg gccgtcgagc
      301 cggtgaaggc cagcttgtcg atgccggggt ggtcgacgat cgcctgtccg gtcgggccgg
      361 cgccggtgat gatgttgacg acacccggcg gcaggtcggc ctgctggcac acctcggcga
      421 agagcagcgc cgtcagcggc gtggtctcgg ccggcttgag cacgacggtg ttgccgcacg
      481 ccagcgccgg ggcgaccttc caggcgagca tgagcagcgg gaagttccac ggcacgatct
      541 ggccgacgac cccgacgggg cgcgggttcg ggccgagcga ggcgtagtcg agcttgtcgg
      601 cccagcctgc gtggtagaag aagtgcgccg cagccagcgg caggtcgacg tcgcgcgact
      661 ccttgatcgg cttgccgttg tcgagggtct cgagcacggc gaactcccgg gcccgctcct
      721 gcaggatccg cgcgatgcgg aagaggtact tgccgcggtc ggcgccactc atccgcgacc
      781 agacgcgggt gtacgccgtc cgcgcggcgg ccaccgcggc atccacgtcg tcgctgctcg
      841 cctcggagat ctcggcgagc acctcctcgg tggccgggtt gacggtcttg aacggcgttc
      901 cgccggtggc gggcacgaac tggccgccga tgaacaggcc gtagctgctc gcgatgtcga
      961 cgacggcggc cgactcgggt gccggtgcgt actcgaaggg agacatggga ttcggtcctt
     1021 tgggcgcgtc gagagggtca gtacaggctg ggggagtcgt cggcgacgta gtcgcccccc
     1081 gagtaggcgc cggtgcgcat ccgctggcgc tgcaggatga ggtcgttgag caggctcgac
     1141 gcaccgaagc ggaaccactc gttggtcagc cacgcctcgc cggcgacctc gctgaccgcg
     1201 acgaggaact tgatcgcgtc cttgctggtg cggatgccgc ccgcgggctt cacgccgacc
     1261 atctcaccgg tggtgtcgtg ccagtcgcgc acggcctcga gcatgacgag cgtgacgggg
     1321 agcgtcgcgg cgggggagat cttgccggtg gaggtcttga tgaagtcgcc gccggcgagc
     1381 atcgacagcc aggaggcgcg gcggacgttg tcgtaggtca cgagctcgcc ggtctccatg
     1441 atcaccttga gccgggcgct gccgcagacc tccttgacct gggcgatctc gctgaagacg
     1501 gtgaggtagt cgccgctcaa gaacgcgccg cggtcgatga ccatgtcgat ctcgtcggcg
     1561 ccggcctcga cggcgtcgcg ggtgtcggcg agcttgaccg gcatgctcgc ccgcccgctc
     1621 gggaacgccg tggcgacggc ggcgacgttg atggctgcac cgccgagggc gtccttggcg
     1681 acggccacca tgtcgccgta cacgcacagc gccgcgggcc gtggggtgct catgtcggtg
     1741 gggtcgggca ccagggcctt ggccgcgagg ccgcggacct tgcccggggt gtcggcgccc
     1801 tcgagggtcg tgaggtcgat catcgagatg gcggtgtcga tggcccacgc cttgctcgtg
     1861 gtcttgatcg agcgggtgcc gagcgcggcg gcccgggcct cgcagccgac ctggtcgaca
     1921 ccggggaggc cgtgcagcca gctggtgagg gcctggttgg tcagccggga caccccgagc
     1981 aggtcgcggg cgcgggcggt ggggtcgacg tcggtggcgg tgctcacccg cggagtctac
     2041 ggcgggtctc agcccgagtg ggcccgtcga gagaaggcaa cacgcaggga acggcgcctc
     2101 acggcctgcg tgttgcctcc tctcgatctg gggacgaggg tcagaggcgg gtcgcggcct
     2161 ccaggtcggc ccggatgccg gccagtcggc tcccagcgcg acctcgcgcg gtcttgacct
     2221 cctcatcggt gccgaccggc tcgatgacct cgaggtacac cttgagcttg ggctcggtgc
     2281 ccgaggggcg cacgatgatc cgcgactcgt cggccaggta gtagcgcagc ccctcggtcg
     2341 ggggcagccc accatcgccc tgcgcgaggt cgtcggcccg ggcgacgtcg acccccgcca
     2401 ccgaggtgag cggggtggcg cgcagccgcg ccatgatctc gccgatgagc gagaggtcct
     2461 cgacgcgcac cgagaaggcg tcggtggcgt gcaccccgtg ggcccgggcc aggtcgtcga
     2521 gcaggtcgtc gacgctgcgg cccccggcct tcaggcccgc ggtcagttcg gcgaagagca
     2581 gcgcggcgct gacgccgtcc ttgtcgcgca cctgcgccgg gtcgacgcag tagccgagcg
     2641 cctcctcgta gccgtagcgc agcccgggca cccgggagat ccacttgaag ccggtcaggg
     2701 tctcctcgtg acgtaccccg gccgcccggc acatcgcggc gagcagccgc gaggagacga
     2761 tcgagttggc gaacacgcac tccgccgagt cgggcagcac cccggccgca gaacccttgg
     2821 cgagcaggtg cgccccgagc agggcaccga cctcgtcacc gcgcagcatc cgccagccac
     2881 cggggcccgg caccgcggcc gcacagcggt cggcatccgg gtcgttggcg atgacgacgt
     2941 ccggcccggt ctgctccgcg agctcgatgg cggcgtcgat cgcgcccggc tcctcggggt
     3001 tggggaagga caccgtcggg aaggccgggt cgggctgggc ctggctctcc acgacgttgg
     3061 gcgccgagaa cccggcccgc tcgaaggcgg ccagcacggt ctcggtgccg accccgtgca
     3121 gcgcggtgtg cacgaccgac agctcggccg cggcgccgtg ctcgaccacg gcggcggcgt
     3181 cacgcaggta gtcctgccac acgtcgtcgc cgagggtctc ccagccgtcg tcggcccggg
     3241 ccacgcacgc caccgacgag acctgcgcga tctgctcggc gatgagggcg tcgaccggcg
     3301 agacgatctg tgacccgtcg ccgaggtaga ccttgtagcc gttgtcctgc ggggggttgt
     3361 gcgaggcggt gaccatgaca ccggcatccg ccccgagcca gcggatcgcg taggcgagca
     3421 ccggcgtcgg catggtgcgg ttgaggatcg ccgcccgccc accggcggcg acgacgacgg
     3481 ccgcggtgtc acgggcgaag acgtccgagt tcgtgcgggc gtcgaagccc acgacgacga
     3541 acggctcggg ggtgagcgtc ttcaggtagg ccgtgagccc ggcggccgcc cggatgacca
     3601 cggcccggtt catccggttg ttgcccccac cgagcttccc gcgcagaccg gccgtgccga
     3661 actcgagcat cccggccatg gcatcggcca gctccgtggc cgcgccctcg tcgccgccct
     3721 ccgcggcgtc ggcgaggccg acgagctcgt cgcgcgtcac ggcatccggg tcgtcggctg
     3781 cccaggcgcg ggcggcggcg agcaggtcgg tcaggtgagc gctcacggca tcctcagatc
     3841 ttggcgacga cggcggcgag gaggcgtccg caacgctgcg cggcctcgcg gccggcctcg
     3901 aggacctctt cgtggttgag cggcgtcttg gagatgccgg cggccgggtt ggtcacgagc
     3961 gagatgccga gcacctcgag cccgctctgg cgggcggcga tcgcctcgag cgaggtcgac
     4021 atgccgacga ggtcaccgcc gaggatgccg gccatgcgca cctccgcggg ggtctcgtag
     4081 tgcggcccgc ggaactgcac gtagacgccc tcgtcgaggt cggggtcgat ctccttggcg
     4141 atggcgcgca gccgcgggga gtacacgtcg gtgaggtcga cgaagttcgc gccctcgatg
     4201 ggggaggtgg cggtgaggtt gatgtggtcg ctgatgagca cgggggtgcc cggcgcccac
     4261 tcctcgcgca acccgccgca gccgttggtg atgacgacgg tgcggcaccc cgcggccgct
     4321 gcggtgcgga tgccgtgcac caccgcacgc acgccgtggc cctcgtagta gtgggtgcgg
     4381 gtgccgtaga ccagggcgcg gcgacccgtg tctccgatgg cgaccgagcg catgacgccc
     4441 gaatggccgg cgacggcggc cttggcgaac ccggggacgt cggtgttgtc gatcgtcgcg
     4501 agggtctcgc cgatgaggtc accggtctgc ccccagcccg atcccagcac gagggcgacg
     4561 tcgtgcgccg ccaccccggt gcgctcgcgg atgaccgctg ccgcggcatc ggcgacggcg
     4621 aacgggtcgg tcgcggggtc ggacagggcg tgggcggcgg cgttgtcggt cacgctgcgc
     4681 agcgtaccga cccgagccca tctgcaccta cgtcaaaact ttgtcgaaga aggtgcatcc
     4741 aagcccgggg tccccgcgga agaaggggtg tcagaccaac ccagagcttc accccccgaa
     4801 aggactcctc cgatgcgacg catccttccc gtggccgccg tcctggccct cgccctgacc
     4861 accgccgcct gtgggagcga cgaggccgag cccgcagcct ccagcgcttc cgcggccgcc
     4921 accaccgagg cgccctcccc gtcgccgagc gagaccatgg cggcgtccga catcgtcgac
     4981 accgcggtcg cggccggcga cttcacgacc ctggctgccg ccctgacggc tgccgagctc
     5041 gtcgacaccc tcaagggcac cggcccgttc acggtgttcg ccccgaccga cgccgccttc
     5101 gagaagctgc cggccggcac ggtcgacacc ctcctgaagg accccaaggg tgacctgacc
     5161 cagatcctca cctaccacgt cgtgcccggc aaggtcatgg ccgctgacgt cgtcaagctc
     5221 gacggccaga aggtcgcgac cgtccagggc ggcgagctga ccgtcaacgt cgacggcgac
     5281 aaggtctcgc tgaccgacgt ggccggcaat accgtcaacg tgacggcgac cgacgtcgag
     5341 gccagcaacg gcgtcatcca cgtcatcgac gcggtgctca tgccgaagtg accccctgag
     5401 gtctccgcag cacccaactg acggatgccg ggtacagcga cagtcgctgt acccggcatc
     5461 cgccgtctgc cccctcaccc cgacttcgtg cgacttccgt gcgggtttcg cgcctcctga
     5521 gccgaaaacc cgcaccgaag tcgcacgaag tcgttgcctc tagtcggcga cggcgagggc
     5581 ggcgtcccca cggggcgaca gggtcacccg gtcgccgggg cggaacgtcg tgacgtcggt
     5641 gtttggcacc tgcaccgtga cgagcgtgcc gtcggggatg cgagcgatga cccggccgtg
     5701 tccgccgagg aagctcgcgc tcacgacgga tgccgtcccg ccgtcgtcgt gcgcgatgcg
     5761 caccccttcg ggtcgcacca ggaccagggc gctcggcccc gaggaggaac cgggcagcag
     5821 gggcacggag ccaccgagca ggtgggcggt ctcgccggcc acctcgccgc ggatgcggtt
     5881 ggtgagcccg acgaagtcgg cgacgaaggg ggtcttcggc gccgagtaga gctcggccgg
     5941 tggcgcgagc tgctcgagct tgcccacgct catgacaccg acccggtctg cgacggcaag
     6001 cgcctcctcc tggtcgtgcg tgacgaacag ggtggtcgtt ccgacctcga gctggatgcg
     6061 ccggatctcg tcacgcagct gggtgcgcac cttcgcgtcc agggccgaga gcggctcgtc
     6121 gagcaggagc acctgcggct cgatggccag ggcccgggcc agggccacac gttgctgctg
     6181 gcctccggac atctggtggg cgaacttgtc ggcgtgttcg accaggccca cgaggtcgag
     6241 catctcggcg gcgcgggccc ggcgtgcggc cttgtcccgg ccgcgcagtt cgaggccgaa
     6301 ctcgacgttc tgccgggcgg tcatgtgggg gaagaggctg tacgcctgga agaccatccc
     6361 catgtcgcgc ttgttcgtcg gcaccttcgt caggtcgttg ccgccgacga cgatggtgcc
     6421 cgagtcgggg tcctccagcc cggccaggca ccgcagggcg gtggtcttgc cgcagccgga
     6481 ggggccgagc agggcgacga actcgcccgg ctccatgtgc agcgtcagcc cgtcgagcgc
     6541 tgcgacgcca ccgtaggaac ggcgcagttc ggtgagctgg acgggggtgc cacgggggga
     6601 gtcggtcatg cggctggctc cttgcggtcg acgcgggtgc gccggaatcg ggcgaggggg
     6661 gaggtgccgg cagggctgct gccgacgaag gacatcgcga acagcacgac gaaggcgaac
     6721 accagcgaga ccagcccgac ggcgaccgag atcttcgcgt cggacttgcc gagctcgtac
     6781 atcgcgacct ggaggttctt cttgctgaac aggctggcga tggtgaactc accgagcacg
     6841 agagccaccg acaggaacga cgccgagagc acggccgaac gcaggttcgg cagcacgatc
     6901 cggcgcatga cggtgaacca cgatgccccg agtgaccgcg cggcctcgga gagggtgcgc
     6961 acgtcgatgg cgcgcagacc ggcatccaac gaccggtagg cgtagggcag gacgagcacg
     7021 acgtaggcga gcgagagcca cagcgtcgac gagccgacga agtagtacac ccaggcgtag
     7081 acgggcgtca ggcccacgac cagcacgatg gccggcacgg tgagcggcag caggcagatg
     7141 aactcaaccg tgcgcgacag tccgggaagg cgcaggcgca cccaggtcat cgtcggcacg
     7201 aggatcgcga gcatgaccag cggcgtgacg atcgcgagcc cgatggaggc gaggaacccc
     7261 tggcgcaggg ccgggtagtc ggcggtgatc gtgctgacgt cgacgagggt gcgccaggtg
     7321 tcggcggtgc gcccgacccc gccggggccc tgcgtcgtga agtcgagcat gccgagcagg
     7381 gggagggcga acaggaaggt ggccaggagg acggcgaccc agcggaaggt ggtctgacgg
     7441 cgacgcaacg tcgcgcgctg gccgctcatc gcggcatcca cttgctcgtg cggcggtcga
     7501 gcatgacgta cgccgtcatg acgagcgcca cgacgacgac catcgcgagc gccatcgcct
     7561 tgccgacgtt ctcctgcccg aggatgacct cggagctcat cgtgccggcg atctgcagcg
     7621 gaagcagggg tgagccctgc gagatcagcg ccttggccgt ggcgtaggcg gagaaggcgt
     7681 tggtgaacag cagcagcatc cccccgacga acgagggcat gaggatcggg ccggcgacgc
     7741 ggcgccagta cgtccaggtg gtgccgccga gggtctcggt ggcctcacgc cactgggggc
     7801 gaaggccttc gaccgcgggc aggaagacga tgaccatgag cgggatctgg aagtacaggt
     7861 agatgagcat gaggcccttg ttccactcgt acaaccagaa gccggtgccg gtgtcgacgc
     7921 cgatggcggc caaccaggcg gtgatgaggc cggtggcccc gaaggacgcg acgaacgcga
     7981 aggccagggc cacgccgccg aactgcgcga ggacgccgca cagcgaggtg atgaagcgct
     8041 tgaccacgct cgtcgcggat gccgtgctga cggcatacgc gaggatgccg ccgacgatcg
     8101 ccccgacgac cgcggtcacc gcggacagga ccaccgagtt gcgcagcccg cgcaccacgg
     8161 acggtgaggt gagcgcctcg atgttggcgg tggtgggtga cccgtcggcg tcgaggaagg
     8221 cgcccacgat gacgacgagc gtcgggatga gcaggaagac ggtggtgtag gcgaagaacg
     8281 ggatgacgcc caagagggca ccggacggtc gacgaccgcc cagtgccctc ttgcgcgagg
     8341 cgtcagccaa cggcgttggc ccagttctcg gcgaggtacg ccttgccggc ctcggcgtca
     8401 gcctgggtcg ggaccaccgc ggcgccctcg atggggggca gtgcggcggc agctgcggcg
     8461 tcgagcgtgc cggcggtctt catcgcctcg aagcggaccg ggcgggcgcc acccttgagc
     8521 cagaggttct ggccctcgtc ggagtagagg tactcctgcc acaggcgagc cgccgcaggg
     8581 ttgggggcgt cggcgttgat cgcctggtag tagaagcccg ccacgacggc ctccttggga
     8641 accatgacct tccaggtcgg cagcttggcc gacaggccgg cgttgaggta gtcccagtcg
     8701 atgacgagcg gggtctggcc cgactcgacg gtggcggggg tcgggtcgac gggcaggaag
     8761 ttgcccgcct gggtgagctg cttgaagaag tcgacgccct tggtgaggtc ggcagcggtg
     8821 ccgccctgcg cgatggagac catctgcaca ccggcgaacg cggcgccggc ctcggtcggg
     8881 ttgccgttga gggcgacctt gcccttgtag tcggccttca gcaggtcggc gacgctggtc
     8941 ggggcgggca ccttggccga gtcgtagccg atgctcatgt agccgccgta gtcgttgacc
     9001 cacaggccgt tggcgtcctt gaggtcggcc gggatgtcgt cccaggtggc caccttgtag
     9061 gccgcgtact tgtcggtgtt ggccagggcg actgcggcgc cgaggtcgaa cacgtcggga
     9121 gcgccggcct ggcccttgag gcgctcggcg gtcgcgatct cgtcggcgct gctcgcgtcc
     9181 ggctggtcgc tggtgatctt gatctcgggg tacttggcct ggaagccctt gatgatctcg
     9241 ccgtagttcg cccagtccgg cgggagggcg atcacgttga gcttgccctc ggccttggcg
     9301 gcggcgacga gcccttccat gccaccgaag gcctcggcgg aggtggcggt cttcgcgtcg
     9361 accgatcccg aggaaccggc cggtgccggg gtctcctcat cggcgggcgg ggaacaggcg
     9421 acgacggcag cggcggcgaa gccgatgacg gccagcgcgc cggcacgtcg ggtgaagggg
     9481 tgagtcatcg ttgacctcct ggtgagtgac acggggggga ctcccgcggg agggccgcgt
     9541 cgagcaggcg acccttcgtt gttggtatga aaccggacct ttgggccttt tggtgggggc
     9601 gagaccaaaa cgtgacctcg cgagcggcat ccggtccagc ggtgaaagga ggtgacggca
     9661 gtggttggat ggtcgctgtg agcaaccggt cgagcaaggt cgtcatcctc ggtggaggtc
     9721 ccggcgggta cgaagctgcc ctggtcgcgg cccagctcgg ggccgacgtc acgatcgtcg
     9781 agcgcgacgg catcggcggg gccgccgtgc tcaccgactg cgtgccgagc aaggcgctca
     9841 tctccacggc cgactacatg agcgacttcg agaccgccca cgggctgggc gtcaacctcg
     9901 aggacgccga gggcgacgag gtctcggatg ccgtcgcgga cctcttcgag gtcaaccgtc
     9961 gggtgcgtgc gctggccgcc gcccagagtg ccgacatctc gacgcgcctg gccgaggtcg
    10021 gggtgcgcca cgtcgcgggc acggcatccc tcacctcgcc cacgtcggtt cgggtcgagg
    10081 gcccggaggg cactgaggac ctcgctgccg acgtcgtgct cgtcgccacg ggagccaccc
    10141 cgcgcgtcat ggacaccgcg cgccccgacg gcgagcgcat cctcacctgg cagcagatct
    10201 acgacctcga cgcgctgccc gagcggctcg tcgtcgtcgg gtccggcgtc acgggtgccg
    10261 agctggccca ggcctacctc ggtctggggt cgcaggtcgt gctcgtgtcc tcacgcgagc
    10321 gggtgctgcc cggcgaggac ccggatgccg ccacggtcat cgaggacgtc ttccgccgcc
    10381 ggggcatgga ggtgctgtcg cgctcgcgga tggccgcggt ggaccgcacg gatgccggag
    10441 tgcgggtgac gctggaggac ggccgcgtcg tcgaggggtc gcacgcgctg ctcgcggtgg
    10501 ggtcgacacc gctgacgcgg gggatcgggc tcgaggaggt cggggtcgag ctgaccccgt
    10561 cggggcacgt cgtcgtcgac aaggtgtcgc ggacctcggt gcgcggcgtc tacgcggccg
    10621 gtgactgcac gggggtgctg ccgctggcgt cggtcgccgc ggcgcagggg cgcaccgcga
    10681 tggcgcacgc cctcggtgac gccgtggcgc cgctgccgtt gcactacgtc agcgccaaca
    10741 tcttcaccga ccccgagatc gcgaccgtcg gggtgtcgca cgccgacgtc gagagcggcc
    10801 gggtgcgcgc cgacgtcatc acgctgccgc tcgcgcgcaa cccgcgcgcg aagatgctcg
    10861 gcatcaccga cgggttcgtc aagctcttcg cccaccgggg ttcggggctg gtcctcggcg
    10921 gtgtcgttgt cgccccgcgc gcctcggagc tgatcttccc ggtgaccctg gccgtgcagc
    10981 accggctcaa cgtggaccag gtggcctcga ccttcacggt ctacccgtcg ctgtccgggt
    11041 ccatcgccga ggcggcccgc cgcctgcacg tcacctactg actgaccccc ccaccccgcg
    11101 aaatcctcct gactccgcgg gcgtctcggg aacctgcgcg ggtgatcatg ggttgacccc
    11161 acagaccctg tgaccccgat ccacccagga ggacacccat ggcacgtcgt gccatcaagc
    11221 tggcggcgct catcaccgtg gcccaacagg cccgcgagta cgcccagaag aaccccgctc
    11281 aggtcaacgc ggcgctggac cggttcgagg tcaccgtgcg cagcaagctt cacccgaagt
    11341 actcggccca cgtcggcaag ggcagccatg cgctgcggtc cgggctcggc atcgcctcgg
    11401 ccccgccgcg tgcgggttcg gtgcaggaca ccccgccccc gcagtactga cccctcggct
    11461 tcaccccgga aggttcacct cgaccccaca tcctttgtgg ggcgatggtg aaccttctgg
    11521 catcgggcga ggaagttccg ccgcagggat ggcccggatt gactatgtcg gtggccctgc
    11581 gcccggtgct acgttcggcg aggccgggac ggccacccgg gatggcatcg gctgagcagg
    11641 cagcccccgg ccgggcggca tgcggctcga ggagcccgga tgcgctcgtt cgtcactcgt
    11701 gtgctggcca tcgtcgccct cgtggtcggg tccacgctgc cggtcctccc ggcacaggct
    11761 gctcccgact cgctcgtgcc cgcgccgacg gagctcacct tcgcctgcgc cctgaagagc
    11821 aacgggcaga tgcgctgggt gcagagcctg tcgtcgtgct cgaagaagga caccagggtc
    11881 accgtcaagc cggggccgac ccgggtgtgc atccaaccct cgggctccac gcgctacgtc
    11941 acgagcttca gcggatgccg tccgcccgcc accgcgctca ccctgcctcc cacgtccggc
    12001 accgtctact tctgcgctgc cgccggaagc ggcgtcctgc gcttcgtcac cgacccgtcg
    12061 ctgtgcctgg ccggtgagac cccgctgcag gcgactccga acgacgcggc gcccacgctg
    12121 tcctcgtcac ttcccgcgac cggttccacc agcgtggcca ccaccgccgt cgtccagctg
    12181 acgttttcgg aggccgtcac ggcgccggcc ggtgcctttg cgttcacgtg cggagtgacg
    12241 ccggtggcgt tcgccctctc gggcgccccc ggcgccgtgc tgaccctgac gccgatgacc
    12301 ccgctccaag agggaacctc gtgcagcgtg accgtcctgg gcggctcggt gagcgacgtc
    12361 gacgccatcg accccccgga cgtcatggcg accaaccccg tggtcacgtt caccaccgac
    12421 gccgccccga cgctcgtctc gtccacgccg gccgacgggg ccaccgacgt cgcggtgggc
    12481 agcgacgtcg tgctcacgtt cagcgagccg gtggcggtcc cggccgccgc gctggacctg
    12541 gtgtgtgcgg catccccggt cggcttcacg gtggaggaca gcggcacgtc gacgatcacg
    12601 gtcaacccgg cggctgccct gccggcatcc gcagggtgtc tgctgaccgt caccgggagc
    12661 gcggtctccg acgtcgacgg cggcgatccg ccggacgcga tggcgggcac cgccacggtc
    12721 ggtttcacca ccgctgacga ggccccgacc gtcgtgtcga cgaccccggc cgacggcgcc
    12781 accggcgtgg gcacctccgc cgggctctcg gtgaccttca gcgagccggt caccgcgacc
    12841 tccgacgcct tcaccctcac ctgcggcggc acccccgtcg cggtgaccgc ggatgccggc
    12901 cccgccacca cgttcgccgt cacccctggc ggacccctcc ccacaggggt gacgtgcacc
    12961 ttcacggtcg tcggtgccga ggtcgccgac gtcgacaccg tcgatccacc gaacgtcgtc
    13021 gacggcaaca ccgcggtgac gttcgagacc gcgacgaaca acgcgccgac ggcggtcgac
    13081 ctgaccccgc gggtcgtggc ggagaactcg ccgagcggca ccacggtcgg tgccctctcg
    13141 acgaccgacg ccgacgcggg cgacaccttc acgtacaccc tcgtgccggg cgccgggtcg
    13201 accgacaacg ccctcttcgc cgtcgtcggc acgtcgctgc agaccgccgc cgggctcgat
    13261 gccgaggacg gcagccggtc ggtgagggtg cgcagcacgg atgccgccgg ggactgggtc
    13321 gaggcggcgt tcaccatcac ggtgaccgac gtcaacgagg cacccaccga cgtcaccctc
    13381 acgcacagca gcgtcgacga gaacgagccc gcaggcacgg ccgtcggcac cctcggcggg
    13441 agcgacccgg atgccgggca gacactcacc ttcgccctgg tcaccaacgg gtgtggcagc
    13501 acctacccgg gcagtgcgtc cttcgccgtg gacggcacga ccctggagac agcggcctcg
    13561 ttgaaccacg aggtgacccc gacgatcaag gtctgcgtgc gcgccaccga ctcggcgtcg
    13621 cccggcctgt ccctcgacga ggccttcacg atcaccgtcg ccgacgtcaa cgacgccccg
    13681 acgaccgccc ctgacgagta cctcggcgcc gtgggcaaca cgatgttcgc cctcaacctg
    13741 cccggcacac ccgctccgcg cgtcgccgtg accggtgccg tgctcacggc caacgacctc
    13801 gacgaggacg gcgacaccct caggaccgtc gccgagaccg tcgcctcgac cgggggcggt
    13861 acagcgacca tcgacgcggc cggcaacttc accttcctcc cgggagtcgg cgacaagggc
    13921 cagaccgact cgttcgccta cacggtcacc gacggcgcgg cgctggcgac cggcacggtc
    13981 accgtgacca tcggcgccac ggtcgtgtgg tgggtcgaca acgcgtcgtc gtcggcatcc
    14041 cccgacggcc gctcgacctc gccgctgacc agcacccagg cgctcaacgg gcccgacgcc
    14101 actgacgtcg actccgcggg cgaccacatc ttcgtgttca ccggcaccgc gacgtaccag
    14161 ggtggtctgc ggctcgagcc gaaccagacg gtgctgagca gccgcaccgg catcaccgtc
    14221 ggccccgacg ccctcgtcgc cccgggagcg acctcaccgg tgctgacgaa cgcggccggc
    14281 aacggcatcg acctcgcgag cggcacgagc gtgtccggca tcgacgtctc cggggcatcc
    14341 ggcgacggcg tcaacggtgc cgggatctcc acgacgtcgg tcggctccga ggtccccatg
    14401 acgatctcgg gctcggtcgg cgacggcgtc gacctctccg gcgcggggtc cggcgcggtg
    14461 accatcgggg cgacgatcac gacgagcggc gcccgctcgc tcgtcgtgag cggacgcacc
    14521 gggtcgaccg tcaccgtctc cggtgcggtc accggcaagg gcgtcgagct gtcgtccaac
    14581 accggcgcaa ccatctcgct caccggggcg ctcgcgctga ccaccacgac gacaccggcg
    14641 ttctccgcca caggaggcgg cacggtgtca acgagcggga gcccgaacac ggcatccgcg
    14701 ggtacgggcg gagccgtcat cgtcgagaac acgacgatcg gcgcccccgg cctgaccttc
    14761 cggtcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gtcatcgtcg
    14881 agaacacgac gatcggcgcc cccggcctga ccttccggtc ggtgagcgcg aacggcgccg
    14941 tcaacggcat ccggctcgtc aacaccggcg ccaccgccgg tctcacggtg acgggcggtg
    15001 gatcggtggc ccaaggcggt gacgtctcgg gcggcacgat cacggcaacg agcggacccg
    15061 gcatcctgct cacgaacacg cgcaacccct cgttcaacaa cgtgtccgtg accaacgtcg
    15121 gcacggcggc cggggtgaag ggcaccggcg tcaccgggtt cggtttcagc aacgggcgcg
    15181 tcgacggcag cgggctgggt ggcgccgaca acgccagcgc caacctcgcc ttcaacaccg
    15241 tgtcggcggg ggtgagcaac ctgtccgggg tggtgtcggt gaccaactcg gtgctggcga
    15301 acgcctacgg cagcggcatc gacatctaca acgagagcgg caccatcagc tcgctcacgg
    15361 tgtccggcaa cgtcgtccag agcagcacgt cgacgaccac gtcgaagggt cacggcgtca
    15421 tcgtgcagtc gctcgggtcg gccggtgggg cgtccgcggt gacggcagcg gcgatcgcga
    15481 acaaccgggt gctcaacttc ccgtccggcg gcggcatcct gctctacgga ggcaacgtcg
    15541 cgggggcgtc ggcgacgatc ctgggtgcga acgccgggag caaggtggtg atatccggca
    15601 acgtggtgcg cggcgcgtcg gacgggaccc cgatgaacac caactgcatc tacgtcagcg
    15661 tggcgggcta cggcaccggg ttcgtcgacg tgcagaacaa cggcaccgtg ggagagccgc
    15721 tcggcgccaa ccggggcaac tgcatctcgg tcaactccac cggcgcggcc accctgacct
    15781 cgctggtgtc cagcaaccgg gttgctcccg gtgccggcca gatcggtggc gcgttcggga
    15841 tcgcgggcgg cgcggccaag cagacggtgg tcggaccgac cgacctcaac tcggccgtcc
    15901 tcaacctcac cgtcgacggc aacgccgtca gcgcgaccaa gagcagcggc atctccctgc
    15961 tgacgcggga cagcggcacc ctgcgcagcc gggtgcagaa caacaacgtg gccgcccccg
    16021 tcgagctcgc gggtgagtcg ggcatcgtcg tcacctcggg cgaccagatc gccggggacg
    16081 ccacggtgtg cctgcagatc ctcaacaaca gcaccgccgg gagcgtcaac tcggtggccg
    16141 gagggaccgc tccgggcatc ggcctgttca agcgaggcac cgtgcagacg acgaacgact
    16201 tcggggtctc cgggctcacc gcgaccccga cctcggcagc cgacgtcgtc acctacgtgt
    16261 cctccgtgaa ctccgggagc gccctcggca gcgggatcta cggaaccttc cgcgcccagg
    16321 tcagcggcaa caactacgtg ccgtgcaccc tgcccttctg agcctccccg accaccgatc
    16381 tcgaccccac gacccgagga gccagcagtg agcgaaccct tcctctccga gatccggttg
    16441 atgtcgttcg tcttcccgcc caaggggtgg gccctgtgca acggccagct gctgcccatc
    16501 aaccagaacc aagcgctgtt cgccctgctc ggcaccacct acggcggcga cgggcgggtc
    16561 accttcgccc tgccggacct gcgcggtcgc gtcgccgccc acgtcggtga cgggcacacc
    16621 ctgggcgagg tcggagggca ggcggcgcac accgtcaccg tgtccgagat gccctcgcac
    16681 agccacatgg tggtcgggac gacagcgacg gccaccaccc gcctcgtcgg gccgaccgtg
    16741 gggctcgccg cgagcaccgg gtcggcggcc tactcgccgc ccgggtcgcc ggtggccatg
    16801 gccgcgaacg ccctgggctc cgccggttcc agccagccgc acgagaaccg ccagccgttt
    16861 ctcaccctca gcttctgcat cgccctccag ggcatcttcc cctcgccgag ctgactcctc
    16921 gaaaggcacg tcatgggaac tccgttcgtc ggcgaggtcc gactcttcgc cgggaacttc
    16981 gcgcccaccg ggtggatgga ctgcgacggc cagctcctgc cgatcagcga gtacgagacc
    17041 ctcttccagc tgatcggcac gacgtacggc ggtgacgggc agagcacctt cgcactgccc
    17101 gacctgcgcg gccgcgcgcc cgtccaccag ggcaccggcc ccggtctctc cacggtcgtc
    17161 atcggcgagg acggcggggc cgagtcggtc acgctcaccc aggctcaagt gcccatgcac
    17221 acccacgcac tcatcggttc cgccgcgaac gccaccgcga ccagcccggc gggtggggtg
    17281 ccgggcacga tgcccgaagt ggccgcttcc gcctacggaa gcgacgcccc cctgaccacg
    17341 ctgtcacccc agggactcgc cccggcaggc ggctcgcagc cccacgacaa catgcagccc
    17401 tacctgaccg tgcgctacat catctccctc ttcggcatct tccccagcca ggcctgacga
    17461 gagcgaagga caccatgacc gacccgttcg tcgcagagat ccgactcttc cccttcaact
    17521 tccccccgaa gggatgggcg tggtgcgacg ggcagatcat gcccatcgcg cagaacacgg
    17581 cgctgttctc gctgctcggg acgacctacg gcggtgacgg gaggaccacc ttcgccctgc
    17641 cgaacctgca gggtcgggtg cccctccacg aggggcacgg gccgggcctg agccagcgct
    17701 tcctggggga atccggtggc agcgacaccg tcaccctcct cgagagcgag atgccggcgc
    17761 acgcgcatgc gctcacggcg tccgccgccg ccggctacgc ccgctcaccc tcgggcgaac
    17821 tgttcgccaa ccagagcggc ggcgtcaact cctacgcccc gccgtcaggc ccgcaggcgc
    17881 tgctcgcgtc gaccgtcacc gcggaggccg gcggtgacgc gccgcacacc aacatggcgc
    17941 cctacctcgt cgtgtactac tgcatcgccc tccagggcat cttcccgcag cgaccctgac
    18001 gtgcccgtca cctcgcggcc ggccctcgcg gcggaccggc ccttcctcct cgctctctac
    18061 gtgcagaccc gcgaggacga gttctcggcg gccgggttgg caccggcggc cctcgccctg
    18121 ctgctcgagc agcagtaccg ggcgcgtgag gcaggctggg cccacacggc tcaaggtgcc
    18181 gaagaccggg tcgtggagcg tgatggccgc cccgtggggc ggctcgtgat cgacaggcgg
    18241 gcggacggca tccgcgtcgt cgacatcgcc ctcgtcgagg acgaacaagg tcgaggaacc
    18301 gggacggaac tgctgcgtgg tgtcctcgac gaggcggatg ccgcgcacgc tccggtcaag
    18361 ctccacgttg tcgccacgag cccggctcgc cggctctacg agcggctcgg gttcgagccg
    18421 gtgtcggcgg acggcgcggg ggagggggtc cacgtcctgc tggagcgacg cccttcggcg
    18481 tgagccccgg ggccaggccc gtcagaggca gttgacgacg gccagcggcc tgccgcgggc
    18541 ggcgacgacg aacagcggtg tggcggtgaa ccccttgcgc gcgaaggtgt acaccccgtc
    18601 gccgggcagc gtgccggtga aggacaccgc gaactgcttc tcgccgccgg ccaccccgcc
    18661 cggcccgagg tcggcgaccg acgcgagcac gaccgaccac cgcccggcgg ccgaggtcga
    18721 ggtgaacgtc gagccgacgt gcggggtgaa cctcgaccgg cgcagcaggt cggtctgctg
    18781 cttcgcggcc tgagcagagg atgccgtggc ggtcgtcatc ggaccggtgg ccgccacggc
    18841 ggcggctccg gtgatgaccg cgcggcgtcg gggtgcactc atggggccct gccttctcca
    18901 gcgggggagg gcacaccccg cccgggtgcg ctcgtgtggc ccagtctgtc gctgcgtgcc
    18961 gcacttgtct gtggcccgaa atgaccatct gcgcggggtt cggccggtgc gggcccggcg
    19021 ccgcggagcg gcatccgagg ccccgacctg catgacgggg gtcaccggcc ggcatgacgg
    19081 gggtcaccga ccacgcaccg cgcgccggtg ggaggatgga ggcactgacc aggaggtgca
    19141 tccagatgga tcttgcagcc ctgctgctgg agtcccagtc gggcatcgtc gaggagagct
    19201 acgccaccct cgagcgctcg cacgtcaacc actacgaggc cgccggggag gagttcaccc
    19261 gccagcgcct cgacgagctc ttcttgctcg tcgtctcggc gatccgagac cgcgacctcg
    19321 cgcaggtgag cgtcttctcg cgacgggtcg ccgaggagcg cttcaaccag ggcttcgaca
    19381 tcggggaggt gcaggctgcg ttcaacgcgc tcgaacagac gatctggcgc cacgtcgtgg
    19441 ccgtcgcgcc cgcggaggac ctcgccgagg cgaccggcct gctgagcacc gtgctcggct
    19501 tcggcaagga cgtcctcgcg cgcacgtacg tctcgctcgc cagccgtcgg cgtgtgccct
    19561 cgctcgacct cacggcactg ttcgcgggca cccagccgcg cgaggtcgag ccctcgtagc
    19621 gctgccgcgc agccaccgtc atccacctcg gcggtccccg acaccgtgcc gacggagatc
    19681 ggcaagccac gtcccgatcc ttgggtcaca ggacgcgccg ccacggcgac aacagggcaa
    19741 gggtgcctgc cgtcatcgtg agcggcatcg ccgagggggg gagcccgcgc agtacggcag
    19801 cctttgcacg tttgacggct cgcccctgcc cacggacacg acgaggaaag tcaccccacc
    19861 atccgcttga ccgcacgcgg ggatgtccga cgggacccgc tcaggcgtcc ttgatctcgc
    19921 agagcacggc accgttggac accgtggcgc cgacctcggc cgacaggccg gtgacgacgc
    19981 ccgcccggtg agcggtgatc ggctgctcca tcttcatcgc ctcgagcacc acgatgaggt
    20041 cacctgcggc gacggtggcg ccctcctcga ccgcgatctt gacgatcgtg ccctgcatcg
    20101 gagcggtgag ggagtcgccg gatgccgcgg cacccgcctt cgcgccggtg gaccgcttcg
    20161 gcgccttctt gcgagcgccg ggcccgccgt ggccggcgcc gccaccgagg gacaggccac
    20221 cgggcaggga gacctcgagc cgcttgccac ccacctcgac gacgatgctc tggcgctcct
    20281 gcgcctcggg ggcgtcagcg gagccgccgg agtaggcctc gatcgtgttg ttgaactcgg
    20341 tctcgatcca gcgggtgtgg atcgtgaact tctcgtccgg gtcggccggg gcgaaggccg
    20401 ggtcggcgac gactgcgcgg tggaagggcg tgacggtcgg catgccttcg atgacgagtt
    20461 cgtcgagggc gcggcgggca cgggccaggg cctcggtgcg ggtgcgaccg gtgacgatga
    20521 gcttggcgat catcgagtcg aacgcgcctg ccacggtgtc gccctcgagc aggccggagt
    20581 cccagcggac cccggggccc tgcgggacgc gcatcgtcgt gacggtgccg ggggcgggga
    20641 ggaagccgcg accggcatcc tcgccgttga tgcggaactc gatggagtgc gcgtgcggct
    20701 cggggtcggg gtaggtcagg gcctcgccgt cggcgatgcg gaactgctcg cggaccaggt
    20761 cgatgccggt gacctcctcg gagaccgggt gctcgacctg gaggcgggtg ttgacctcga
    20821 ggaaggagat gtcgccctgc ggaccgacga ggaactcgca ggtgccggca ccgacgtacc
    20881 cggcgcggcg caggatctcc ttggaggccc gcaagagctc ggtgtgctgc tcgtcggtga
    20941 cgaacggggc cggggcctcc tcgacgagct tctggttgcg ccgctggagg gagcagtcgc
    21001 gggtggagac gacgacgacg ttgccgtgct ggtccgccag gcactgcgtc tcgacgtggc
    21061 gggggtggtc caggaagcgc tcgacgaagc actcgccgcg accgaaggcc gtgacggcct
    21121 cgcgcaccgc cgactcgaac agctcgggga tctcctcgag gttacgggcg accttcagcc
    21181 ctcggccacc gccgccgtag gcggccttga tggcgacggg cagcccgtgc tgtttcgcga
    21241 actcgacgac ctcgtcgggg ccctcgaccg ggtcgggcgt gccgggaacc agcggggcgt
    21301 tggcagcgag ggcgatgtgg cgcgccttga ccttgtcgcc gaggctgtcg atggcctcgg
    21361 ggccgggccc gatccacacc agaccggcgt cgatgacctt ctgcgcgaag ccggcgttct
    21421 cggcgaggaa gccgtagccg gggtgaacgg catccgcacc cgcctggtgg gcgacctcga
    21481 tgagcttgtc ctgcagcagg taggagtcgc cgggcgtcgt gcccccgagg gcgtaggcct
    21541 cgtccgcgac cttgacgtgg agggcgtccc ggtcggggtc ggcgtagacg gccacggatg
    21601 cgatcccgtg gtcggcgcag gcgcgggcga tgcggacggc gatctcaccg cggttggcga
    21661 tgaggacttt gctgatggcc atgcttcgga ctctagcgtg accagcggcg cgatcgaccg
    21721 gtggtggggc ggcgctcgtg ctgcggtgtg acgtactcca cgagcggtcg gtcgtcgatc
    21781 cgcgaacggt cggttccgac gccgagtgcc ccttcctgac gagtggggaa ggagcggctc
    21841 gatcgcggcg gctgagtgtc cgaactcatg gcgcgacctc gccgggcagg aggacgatga
    21901 aggtgcaccg cgctgggtga tgcaccaacc acgaggagcc cgtgatgacc gcaccgccca
    21961 ccatcccccc ggacgcaccc gccggttcac ccggccagcc cggtcagccg ggtcccgctc
    22021 cgcacgcccc cgtcgtcgcc gcgacccccg gacagctgca ctggctggag ggcgaactgg
    22081 cccactggca gtccgaaggc ctcgtcgacc cggtcgccgc gtcggcgatc cgcaaccgct
    22141 acgtcgcgag ccggcgcttc accctctccc acatcgtcct caccctcggg gcctgcttcg
    22201 tcgggctcgg cctgatctgg ctcgttgcgt cgaacctgga ccagatgtcc ccgctcgtgc
    22261 gcttcctgct catggtggtg atctggctgg gcctcatcgg taccgccgaa gtgctcgccg
    22321 cgcgtcgcga gcgcgagggt gacacgtcat cccccgtcgt gggtgcggtg cgccttctcg
    22381 ccgcgggtgc cttcggggcg gtggtcttcc aggcggcgca gagtttgcag gtgccggcgt
    22441 tcgagccgat cctcgtcggg gtgtggggtc tgggcgccgt gctgtgggcg tatgccgtgc
    22501 ggggggtggc gccgctcgtt ctcggcatcg gcctgatcgc cttctggttc gtctggcagg
    22561 tcatgagtgc cggggagggc gccttcgccg tctcgaccgc cctcgcggcc gctgccctcg
    22621 ccggggtctc ggtcggcgtg gtgcacagca tggtcggctg gcgtgacttc gccctgccgt
    22681 ggcgcgagat cggggcggcg ctgggcctgg tggcgctgtt catcgccgcg ttgccgttcg
    22741 cctggggtga ggctgcgggc agcatcgcgc tgtggctcgg cctcgcggtc gcgggcggtc
    22801 tcgccgtgct ggccctggcc cgcggagacc gcgtcgaccg gttcgaggtg gccctgtcgg
    22861 cgctggcgct ggccctgacg atcgcgctgt cgctgtggcg cttcgaccag gacgtcatgg
    22921 acacggccaa cctgccgccc ggagcgtggg cacgtgccgt cttcgccgtg gtcgcctacc
    22981 tgttcgtcgc gagcgggtat gccgtgctcg gcgggatgcg ggacacgagc cgtctcacgt
    23041 ggttggcgac ggccgccctg gtcatcttcg tcaccgtgca ggcgttcgcc gtgttcgctc
    23101 cgatcgtgtc gggtgcggcg ctgttcctca tcgtcggcct cgtcctgatc ggcaccggca
    23161 tcctcgccga ccggggccgc cgacggctcg tgagcgagac caaggaggcc ctgtcatgac
    23221 cgccctcgag agcggcatcc ggcctgatcc ggcgcagccg gtgcccccga cggggcgcag
    23281 ccgaaccggg cacctcgcac gcgtcgcggg ggtggtgctc accagcctgg cgctgctcgc
    23341 cgtcgccgtc tggccgcagt tgtcggcgcg gttcaccggt gacgaggtcg tgctgcgggt
    23401 gcagccggtc gacccgatcg accccttccg cggtgcctac gtcgatctcg gctaccccga
    23461 cctgccccgc cagcagttcg acgaggtcaa cccggtcacc gaggaggagc agcaggccct
    23521 ggacgcccaa cgcggcatcg cctacgtgcc gctgcgccag cagggagagg tctgggtcgg
    23581 tggcgaggtc gtgcgcacgg cgccggctga cgggctgtac ctgacctgtg acgactccag
    23641 ctggcgactg cggtgcggga tcgagagctg gttcctgccg caggctgatg ccaaggcctt
    23701 cgaggatgcc gttcgcgacg gtcgtgcggt ggccaccgtt cgcgtcgacg gatcggggca
    23761 cgccgccctg gtcgacgtgg agacccgctg acctgaacgg ctggtgtcct ggggcccggc
    23821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgagcgatga cgccgtggac
    23941 cgggcccagc gagtgctgga agaaggcatc tccaccgact tcagcaaggc gatgtcctac
    24001 ggcgcctacc tggacctcga caccttgctc gccgcacagc atccgcgcag tgagccccag
    24061 cagcacgacg agctgctctt catcatccag caccaggtcg ccgagctctg gctcaagctg
    24121 ctgctgcacg agatgcactc ggcccgcgcc ctcatcgccg ccgacgagct cgccccggcg
    24181 ctgaagcggc tggcccgggt caagcacgtg cagaagcagc tcgtcgagca gtgggacgtc
    24241 ctggccaccc tcaccccgag cgagtacgcc cagatcaggc ccttcctcgc gacgagctcg
    24301 gggttccaga gccaccagta ccgcgccgtg gagttcctgc tcggcaacaa gaacgccgac
    24361 atggtgcgcc tcttcgagca cagcccccac gagcacgcgg agctgatgcg cctgctcggt
    24421 gaaccgacga tctacgacga gttcctgcgc ttcctcgccc ggcgtggcta cccggttcct
    24481 tctgcggtgc tcgagcggga cgtcacccag ccctaccggc tcgatgagga cctcgtcgcc
    24541 gtgttcgagg cggtgtacgc ctcgccgtcc gagcactggg gggtctacga gacctgcgaa
    24601 gagctcgtcg acatcgagga cctgttccaa cagtggcgct tccgccacct ccaggtcgtc
    24661 acccgcacca tcgggcaccg gcagggcacc ggtgggtcga gcggcgtcgg cttcctgcgc
    24721 aaggccctgg acctcacctt cttccccgag ctgtacgcgg tgcgcgccct cgtggagtga
    24781 gctgcccttg cgtccggcat gttggctgcc caaggcacca acctgccgga cgcgagcagg
    24841 gatactcagc ccatgcctcg cgtcatccac accgctcagg ccctcgtcga cgccgtgatg
    24901 gaggtgccgt cgctgccggt gcggggcggc aacgtcatgg cgacgtcgta ccgtcgctac
    24961 gccgggggag cggtgacgat cctgctcgcc gcggcgcgct cgggcgtcga gtgcgtgcac
    25021 gcaggggccg tcggcacagg gccgaacggt gacctcgtcc ggggggcgct ggctgctgag
    25081 ggggtgacgt tgtcggcgcc accggtggcc gacctcgaca cgggtgtgtg cgtcgtgctc
    25141 gtcgagccca gtgccgaacg caccttcgtg accacccagg gtgccgagcg gcagatcacg
    25201 gtgcagtccc tggccgcctc gaacccggtg cccggagacc tcgtgtgcgt cagcggctac
    25261 tcgctggtgg gccgcacgcg tgacccgctc gtcgagtggc tggacaccct ggccgacggt
    25321 gtcgtcgtcg tgctcgatcc cggagccacc ttcgccgggc tggccgagcc gctgcgtgac
    25381 cgggtgctcg cccgcaccga tgtctggacc ggcaacgccg acgaggcagc cgcgctcacc
    25441 ggggtgcggg ggatgcgtga ggccgctgag gtcgtcgcca ccctcctcgc accggatgcc
    25501 gtgacgatcg ttcgcgacgg cgctcgcggg tgcgcggtgc gtgagtcggg ccgcacgacc
    25561 cactgtccgg gacacccgca gcgaccggtg gacaccaacg gtgcggggga cacccacacg
    25621 ggggtgctgc tcgccgagcg ggccacgggg gcatcgtggc cggaggccgc tgcccgggcc
    25681 aatacggcag gggccatcaa ggtgacccgt cgtggcccgg acacggcgcc gacccgggca
    25741 gagatcgacg ccttcctggc agccctcccc cgctgacctg cgcggggtcg aggtattacc
    25801 gcacgtggat ccctgcagaa atacctcgaa tcagcggagg gtgcccggct cgtccaatcc
    25861 ctcgaggtcc tcgcggcagc gtcgctcgaa gtccacgagc tcggccagcg ccgactcgag
    25921 gtctcggcgc cgctgctcga ggtcgctgcg gcgctcgccg atctgggcca gcacgtactc
    25981 gagctggctg cgccgcccgc gtggcacgtc gtagaggtcg atgatcgtgc ggatctcgtc
    26041 gagtgggaag ccgagccgcc gcccgcgcag gatgagcccg agtcgggtgc ggtcgcgccg
    26101 gtggtagacc cgggtggtgc cgcgccgctc gggggagatg agcccgaggt cctcgtagtg
    26161 ccgaacggtg cggtgggtga cgccgaactc gtccgcgatc tgcgcgatgc tccaggtccc
    26221 ctcgcccgtc tcggtgcgcc cgcgacgacg tcccgccgcg ctctcgcgtc cgctcccagc
    26281 ggtcggcgag cggacggcat ccgtcgtggg gcttgtcata ccgggcagta ttgctttacg
    26341 ttgacgtcaa cgtcaagaac gttgccaccc gctgttcgcc gcgacccggc gccggcacca
    26401 ctgcgcgaag gggcgccgaa tgttcgagct gagcaaggac cacgaggact tccgggcggt
    26461 tgtgcgcgac ttcgccaccg aggccgtcga gccacacgtc gcccagtggg accgtgacca
    26521 ccacttcccg gccgacctcg tgccccgcat gggcgagctc gggctgttcg gcctcgtcgt
    26581 ccccgaggcc tacggcggct cgctcgagga cgccgacggc ggcgccttca cgagcctgtg
    26641 catcgccatc gaggagctcg gccgggtcga ccagtcgatc ggcatcaccc tctccgcggg
    26701 ggtggggctc ggcatcaacc cgatcctcac ctacggcacc gacgcgcaga aggagcgctt
    26761 cctgcccgac ctcgtcgctg gccgcgcgct cgcgggcttc gggctcaccg agccggatgc
    26821 cgggagcgac gcgggtgcca cgcgcaccaa ggcggtgctc gagaacgggg agtgggtggt
    26881 cgacgggtcg aaggctttca tcacgaactc cggcaccgac atcacctcgg tcgtcacggt
    26941 gacggcccgc accggcaccg acgccgacgg ccgccccgag atctcggcga tcatgatccc
    27001 ctcgggcacc tccgggttca ccgtcgaggc gccctacgac aagctcggct ggaacatctc
    27061 ggacacccac gggctgtcct tcgccggctg ccgcgtgccc gaggagaacc tgctcgggca
    27121 gcgtggccac ggcttccgcc agttcctcaa gaccctcgac gacggccgga tcgcgatcag
    27181 tgcgctggcc ctgggcctca tcacccgcat gcgcgaggag gccaccgcct acgcgaagac
    27241 ccgcaccgcg ttcgggcggc cgatcggggt caaccagggc gtcgcgttcc ccatcgccga
    27301 cctgctcgtc atggaggaga actcgcgcct gctgacctac cgcgccgcgt ggctcaagga
    27361 cgagcaggag gccgggcgcc gctcggtcgc cgacgtcaag caggcggcgg ccgtggccaa
    27421 gctctacacc tccgaggccg ccgtcacggc gacccggatc gccacgcagg tgttcggggg
    27481 caacggcttc atggaggagt accccgtggc tcgcttctac cgcgacgcga agatcctcga
    27541 gatcggtgag ggcacgtccg aggtgcagcg catggtcatc gccaagtcgc tcggcctgcc
    27601 gtcatgaccc agcccggcgg gggcgacccg cgcgtcgcgg acgtccgggc gcggctggaa
    27661 gccgcgcaca cggcatccga gcgcccgacc gagaaggccg ccgccaagct cgcctcccag
    27721 aacaagctct acgtgcgcga ccggatcgcc ctgctcatcg acgaggacag cttcgtcgag
    27781 gacggccgct acgcgaacag tcgggcgccg ggtctgccag ccgacggcgt cgtcaccggg
    27841 cgcgggacgg tcgacgggcg ccccgtcatc gtcatcgcca acgacccgac ggtgaaggcg
    27901 ggctcgtggg gtgcgcgcac cgtcgagaag atcgtgcgcg cgaccgaggc agcgctgcgc
    27961 gaggaactgc ccgtcttctg gctcgtcgac agcgcgggcg cgcggatcac cgaccaggtc
    28021 gagatgttcc ccgggcgccg aggggcgggg cggatcttcc accaccaggt cgccctgtcg
    28081 ggcaaggtgc cgcagatctg ctgcctgttc gggccgtcag cggccggtgg ggcctacatc
    28141 ccgagcttct gcgacctcat catcatggtc gagggcaac
//
LOCUS       DAOSOC010000012        28173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1690_length_28173_cov_8.447116, whole genome shotgun sequence.
ACCESSION   DAOSOC010000012 DAOSOC010000000
VERSION     DAOSOC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 28173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28173
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1690_length_28173_cov_8.447116"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="PL014_01985"
     CDS             complement(<1..1721)
                     /locus_tag="PL014_01985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOT55893.1"
                     /translation="MSPTAAKKAATSKSASEYTAHHLQVLEGLEAVRKRPGMYIGSTD
                     SRGLMHCLWEIIDNAVDEALGGHCDRVEVTLHGDGSVEVRDNGRGVPVDIEPRTGLTG
                     VELVYTRLHAGGKFGGGSYTASGGLHGVGASVVNALSSRLDVEVDRGGKTYAMSFRRG
                     EPGVFSDVAGAKGAKDPSHEFTPYVDISELEVIGRTRRGVSGTRVRYWADSQIFLKDA
                     TFDLEGLLARARQTSFLVPGLTIVISDQRGDEVREEVFVHDGGISEFCEYLAPDPAVT
                     DVWRLHGSGTFTETVPVLDANGHMSPTEVERECGVDVAVRWGTGYDTKVSSFVNIIAT
                     PKGGTHLAGFEQALLKVLRKQLEANARRLKVGNDKLEKDDALAGLTAVVTVRLAEPQF
                     EGQTKEVLGTNAVRAIVARVVEKELTERLLSTRRGEKQQAALLLEKVTAEMKSRISAR
                     LHKETQRRKTALESSTLPAKLVDCRSTDVERTELFIVEGDSALGTAKLARNSDHQALL
                     PIRGKILNVQKASVTDMLGNAECAAIIQVIGAGSGRTFDLESARYGKVIIMTDADVDG
                     AHIRTLLL"
     assembly_gap    2137..2236
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2273..2725
                     /locus_tag="PL014_01990"
     CDS             2273..2725
                     /locus_tag="PL014_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOT55894.1"
                     /translation="MLIIVGLLGIVVPVLPGLLLILGGVLLWAALEGGTTAWLILAAS
                     LLVAVIGWVLQYTLPGKRMRDRGVSNSTLVIAVMVGIVGFFVVPVVGAIIGFVLAIFV
                     VEYLRSKDTALAWERTKHALVAVLHSMGIELAAGLTIAALFVGGLVAT"
     gene            complement(2813..3202)
                     /locus_tag="PL014_01995"
     CDS             complement(2813..3202)
                     /locus_tag="PL014_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HOT55895.1"
                     /translation="MPLSDHEQKMLEQMEQALAAEDPKFASQMQGSSLASMQRRRWLI
                     GAVGIVLGLALVLVGVNTTMWVGAIGFAVMVTAAVFAATPPRRAALGVVRGDGSTAPH
                     SSGGRKKGQKSFMDRLDDRWEKRQNNW"
     gene            complement(3260..4516)
                     /gene="dinB"
                     /locus_tag="PL014_02000"
     CDS             complement(3260..4516)
                     /gene="dinB"
                     /locus_tag="PL014_02000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT55896.1"
                     /translation="MSRRQYALPHRDASEPPDDTGCTVLHVDMDAFYAAATLLSHPEL
                     VGTPVIIGGGNRGVVLSATYEARRFGVTSAMPMSRARRLCPQAVVLPPDHARYSAISA
                     AVMETFRSVTPVVEPLSLDEAFLDVSGAVRAMGSPATIGQHIRDTVHDEQGITCSVGV
                     ASTKFVAKIASGLAKPDGMVVVPRDEVVPFVQQLPVGALWGVGERTEDALLRLGLRTV
                     ADIAHTPLATLVRGLGEATGRHLHDLSWGRDPRSVEHVQREKSIGSDQTFEHDIDDSA
                     EIHRRLLALSERTASRMRAAGLTGRTLTLRVRFSDFTTITRSKTLREPTDSGRAIHEG
                     AVALFDALGLQRARIRLVGVRMEKLMEASASPIQGVIGEPEHGWRDADRAVDRARSRF
                     GSGSVRPASLIERDTPTSAGRHRDLS"
     gene            complement(4535..5338)
                     /locus_tag="PL014_02005"
     CDS             complement(4535..5338)
                     /locus_tag="PL014_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT55897.1"
                     /translation="MADERARSRRAGGPPGLRTAAVHGALDAFAARRSAELGRPLRVL
                     DLGGGTGGTAVPLAVAGHDVTVVDPSPDALASLRRRAAETDTSRRIHPVQGDTDTLRD
                     LGVGGELAADGEPAAYDLVCLHGTLEVVDDPDAAMAGIATVLAPGGTLSLVVAQRLPA
                     ALARALAGQFARAQAILERPDGRWGDDDPAPRRFDENVVLDLLRTHGFTTVDSHGVRI
                     FSDLVPSALVDSEADRAALLELEEVASRHLGHPLLGDLGSVRHVLATRG"
     gene            complement(5393..9319)
                     /gene="dnaE"
                     /locus_tag="PL014_02010"
     CDS             complement(5393..9319)
                     /gene="dnaE"
                     /locus_tag="PL014_02010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT55898.1"
                     /translation="MDGFAHLHVASGFSMRYGASMPEELVERAVAHGQGALALTDRDG
                     LYGAVQFATACGRAGIDPVLGVDLAVDVGTGGSVGGSSSGGGVTAGRSTWDGSARPDA
                     VSHPQRPRTPVRGGAIVDPRRPRVSVLARGQVAGVAPGAGWAALCRLVTQTHLRGERG
                     VPVTTRDLLATWAHDVGEAGGPSRLLVLLGPDSDVGRAALAGRRDRARHLLAAWQTVL
                     PRDGLAVEVVLHGGPEGTSGCRSHAARLLSLADAAGIPAVLTAAVRHVDPDQVRTVDL
                     LDAARRLVVLDSRHLDRVTDSGHLASTAQMRANALEVTGGDRSRADRLVAMTALVAAE
                     CTQQARPDLGIGAVHLPEAQVLGLSAADDPMAVLAQRCRDGVARRYPGAGEAERQVVL
                     DRLDEELAVIETLGYPTYFLTVAEVTDLIRARGVRVAARGSGAGSLVNHLLGISGVDP
                     IRHRLLMERFCSPLRAELPDIDVDVESARRTEIYEAVLTRFGGERVTCVSMMDTYKVR
                     HAVRDVGAALGLPPTEVDEIAKAFPHIRAKDARHAIADLPELRSRGLDSPRMAAFFDL
                     VEALDGLPRHIALHPCGIVLSNSGLLDRTPVEASWLGFPMSQFDKDDVETLGFLKLDV
                     LGIRMQSAMAHAVAEVERVSGEHIDLDDEEQVPHEDPATYELIRTTRTLGMFQIESPG
                     QRELIGKLGPRRFDDLIVDISLFRPGPVKSDMITPFLNSRHGWSTRRFIHPSLIPALE
                     ETEGVVVFHEQVLVIVAETTGVSLAQADEVRRSMGTPQGQVDVEAWWRPAAAARGYTA
                     EDRDRIWEVLKSFASFGFCKAHAAAFALPTFHSAWLKTHHPAAFLAGILTHDPGMYPK
                     RLLLDDARSFGIPILGLDVNASTDTYRIEQVSEPTPDAVEPDAGVPYAGVLDAVEPAW
                     QRANQPYGIRLSLADVKGISAAEVARVVAGQPYADLGDFWTRARVSRPVVERLVLAGA
                     FDSLHGMGDGGLGRRGRVTRRDLLLHVAELERWDRGSGGPARRSRRLAVPSIAPPRED
                     GAAALAGLPLDVREQAAAQSQAAREVVPAAAQPTQLTLDLGDRPRLVPGAGLPELTGP
                     EKVRAELDVLGLDVSAHVVQFYDPLLRALRVTRSVDLLATRSRSRVWVAGVKVATQTP
                     PVRSGRRVVFLTLDDSTGPADCTFFEDVQGPYADVVFHSWLLLVRGVLRRTGDRGVSI
                     RATGAWELAGLWDAWQLGGLERVQAVVAAGEADAVARAEAARLAAIEQDARTGRRVLV
                     HASGFRQSPYADTRPPGLDPRSGRGAPVDALPPRKLWHASPGSSGH"
     gene            complement(9327..9758)
                     /locus_tag="PL014_02015"
     CDS             complement(9327..9758)
                     /locus_tag="PL014_02015"
                     /inference="COORDINATES: protein motif:HMM:NF037499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV_6107 family HEPN domain-containing protein"
                     /protein_id="HOT55899.1"
                     /translation="MSHAATTLDLLDRSRESLTSGYAASAASARYLAASLAALRAGAA
                     IVAARAATHPGAGLAARGNGPHDVWALTARVAPELTEWAQRFAASTGQRVGVETGLVR
                     VSAREADDLLRDAETFVDLAARRLGVPGVQRPQRLVPVRSA"
     gene            complement(9855..10199)
                     /locus_tag="PL014_02020"
     CDS             complement(9855..10199)
                     /locus_tag="PL014_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HOT55900.1"
                     /translation="MVRRHDEHISVRSSSDLGVGGDPLGEPRVEPRVGADGPRDFVWR
                     GRRYTVCAVLASWQERRAWWREVGDLTGGAALPSPQRRVWRVEARPPSGTAGVFDLGL
                     DDDRWLLLRAHD"
     gene            10366..10890
                     /locus_tag="PL014_02025"
     CDS             10366..10890
                     /locus_tag="PL014_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOT55901.1"
                     /translation="MTQPPSEQNPPRDLSEHAGVRSVMQALAEHGLSPQVRHLPDAVR
                     TAQAAADALGISPAEIANSLVFVATTHDGERLPLLVMASGGHRVDVVSVAERAGLASI
                     ERADPAFVREHTGFAIGGVAPVGHPHPLRTIIDTHLATYPVVWAAAGHSHSVFATSYD
                     DLVRITGGQPMDVA"
     gene            11138..12580
                     /locus_tag="PL014_02030"
     CDS             11138..12580
                     /locus_tag="PL014_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HOT55902.1"
                     /translation="MYLTISTTHRPATDLGYLLHKHPDRVQQFTQSFGVATVFYPQAT
                     DERCTAALLLEIDPVRLARGRARNLPDFSLGQYVNDRSYAASSLLAVTLAEVFSTARI
                     GRCASHQDLADSPIPLQIRIPVLPCRGGPAIAHRIFEPLGWQVVADPIDLDEAFPQWG
                     ASRYVDLTLTGTVRLADALTQLHVLLPVLDESKHYWQGPDEVDKLLRSGGDWLAGHPE
                     AELITRRYLSRTGYTRVALERLAELGDDAPTDDPAEDWCATPTSDAVEPVASPVTTEP
                     LTTARTPLNRQRHDAVLAELLNLGVSSVIDLGCGPGAFLERLVRTPSFTKVAGSDVST
                     RSLHQAARRLHLDTMTERQADRVELFQGALTYEDPRYAGFDAAVLMEVVEHVDPSRLP
                     ALESVVFGTASPGAVLVTTPNREHNVHYDGLTGMRHPDHRFEWDRDEFATWCARVAAT
                     HGYSVVIRGIGDPEAATGHPTQLGVFTRDA"
     gene            12570..15095
                     /locus_tag="PL014_02035"
     CDS             12570..15095
                     /locus_tag="PL014_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HOT55903.1"
                     /translation="MTPETSDTTEVRVPASGLVVLVGVSGSGKSSFAREHFRPTEIVS
                     SDACRAMVSDDENDQSATPDAFELLNHIVGTRLRRGLLTVVDATNVQSKARAELVSLA
                     RRHHALVDAIVLEVPESVAGRRNAGRADRDVGMPVVRRQQRDLSRSKKRLRKEGFRQV
                     HVLDAGGAPPRVQIVRERPWNDRSEVHGPFDIIGDVHGCLGELRTLLEDLGWALDFDD
                     AGRAVGACHPQGRLAVFVGDLVDRGPDSPGVVRLVMGMVEQGSALCVSGNHEAKLVRA
                     LRGTTVTVSHGLAQSLEQFGREPDQFRADALAFMDGLVAHYVLDDGRLVVAHAGLKEE
                     YHGRTSARVRAFALYGDTSGETDEYGLPVRYPWAQDYRGKAMVVYGHTPVPSAQWLNN
                     TICLDTGAVFGGALTALRYPERQIVSVPAQQQWYEPVRPLGVPSPDRQPSVLRLDDVS
                     GTRWLDSTRAGRVKVEEENAAAALEVMSRFAVDPRWLVYLPPTISPARASTREGYLEH
                     PQEAFDEYAGWGVTSVVCEEKHMGSRAIAVLTRDAAVAARRFGIDDGSSGCVYTRTGR
                     PFFSDTAPLVEAMRDAVAPLFASLDTDWVALDCELLPWSAKALDLITDQYASVGAAAR
                     HALPAALDGLDAASGRGLDVEDLRRRTADRHTNALAFREAYAAYVRPTDGLEGVTLAP
                     FQVLAVEGRLLAETHPHPWHLAQLAGLDSDLITPTRHRIVDLTADREREDAVRWWEEL
                     TAAGGEGMVVKPAHVVTGRAQPGLKVRGREYLRIIYGPDYTDALPLLRERNLTHKRRL
                     ALREHGMGLDALAGFVAGDPLWQVHQRVFAVLALESEPVDPRL"
     gene            complement(15114..16019)
                     /gene="metF"
                     /locus_tag="PL014_02040"
     CDS             complement(15114..16019)
                     /gene="metF"
                     /locus_tag="PL014_02040"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOT55904.1"
                     /translation="MAHYGGGGRPAHLVASIPGRLAQPRPSISFEFFPPKDDASEAQL
                     WTAIRDLERLRPAFVSVTYGAGGTTQDRTVRVTGRIADETTLTPLAHLTCVGASKRAV
                     RQVVGAYAASGVRNILALRGDPPGNVRGQWTPHPEGLDHADQLVALIRGLGDFTVGVA
                     AFPDVHPDSPDLGHDVDVLVRKADAGASFAVTQMVFDADSYLRFRDLVAARRDLPITP
                     GLMPVTNLKQIQRMSELMGTPLPPAVVERLEAVKDDPAAVRAVGVEIATEIGQRMLDE
                     GAPGLHFITMNRSTATLEVYENLSL"
     gene            16122..17207
                     /locus_tag="PL014_02045"
     CDS             16122..17207
                     /locus_tag="PL014_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT55905.1"
                     /translation="MASPLDTADLRHRVQDCLDAELTTQAAVLAELGPDVDELVTAIS
                     ALLRGGKRLRAAFLYWGHRAAGRPDSDALIRLASSMELFQAAALIHDDVMDDSDTRRG
                     MPAAHRAFASAHGGRGWGGDSDRFGLAGAVLAGNLCLTWTDEVYATCGLSESDLARGR
                     TVFDRMRSQLMAGQFLDVVESMRPWDGLPAAERIERADRVIRYKSAKYSVEHPLLIGA
                     TTGGLDDAGVEALSRYGLDLGRAFQLRDDLLGVFGDPQETGKPAGDDLREGKRTVLLA
                     HALAGLDAGGQARVESLVGRADLDASQVEELRATIVGSGAVARLEDEITRLADAATSA
                     LGDATSIGDEARAALLDLVGITTARTA"
     gene            complement(17251..18267)
                     /locus_tag="PL014_02050"
     CDS             complement(17251..18267)
                     /locus_tag="PL014_02050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT55906.1"
                     /translation="MVVDEQALDDAVAAQAFTGVVTVDVGDRRVLERCEGFVHRALGV
                     PMTADARFGIASGSKAFTALAVMCLVEQGVLGLSDRVREWLGDDLPLVDDGVTIEHLL
                     EHTSGIGDYLDEDGGWSPSQFVMTLPVHTLTTAQAFLPMLDGHAQRGEPGLRFTYCNS
                     DYAVLAVVLERATGQGYHDVVRRLVLEPAGLEHTGFLPLNDLPGDVALGYLNPEGNLV
                     NTLHLPVLAAGDGGAFTTATDLHRFWGALTAGQIVSPATLARMTAARHDVPEEGMRSA
                     MGFWLHRSHPALIIEGYDAGVSFRSTHLVESRTTVSVLGNSSEGAWPVIATLARDIDV
                     ELAR"
     gene            complement(18335..18745)
                     /locus_tag="PL014_02055"
     CDS             complement(18335..18745)
                     /locus_tag="PL014_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HOT55907.1"
                     /translation="MNETPQSETNDAAAAAPGADLEALVGEWLTVPDIAERLGLPLST
                     VRRLIEDRELLSHRIGERRVVAVPAGFLTDEGPHHALKGTFTVLADGGMTDIEIIRWL
                     HTPDETLPVPGTPLNAIHAGFKTEVRRRAMEEAF"
     gene            18847..20817
                     /gene="pknB"
                     /locus_tag="PL014_02060"
     CDS             18847..20817
                     /gene="pknB"
                     /locus_tag="PL014_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOT55908.1"
                     /translation="MSSNGDALIGRTLDGRYRVLRRLADGGMATVYLALDERLGREVA
                     LKVMREHLVHDESFVTRFHREARSAASLSHPNVVAVYDQGRDGDVMFLVMEHVPGLTL
                     REVMREEGALTPRAALDILEPVLLALGEAHANGLIHRDVKPENVIINDNGTVKVADFG
                     LARAVTSQTVTSTSGVLLGTVAYLSPEQVERGIADARSDVYAAGLMLFEMLTGTKAVT
                     GDTAIHVAYQHVHGGMPLPSSRVPGLDPALDDLVAAATARDPDDRPADAAALLTMLRR
                     TRALLSPAALDSRPAGAEALAAAALPTTAAASPAATAALPMPGSHSATASGSVTTRPH
                     TDTRALTLPASPPSPPAPLEGRVVVGGGRSGGTRRWWPAIAASALIAALTAWFFMLGP
                     GAMATVPSVLGQTEAAAVAAIDDAALTPTVTQAFDEVVPAGQVISTDPGAGETIRRGA
                     GVSVVVSKGPERYEVPPVVGMTLTEATASIAEVNLVVGEVEEVFDETVPKDEVISADP
                     GPGASLKKGVEVNLTVSKGRKPIEVRDYTGKDAAAAQKSLTDLGLKVDASKEENSDTV
                     AKGMVISQSPNDGTLFKGDTVTLVVSKGPVMVDVPNVVGQQVDQARAALEAAGFKVTV
                     RKALGGFFGTVRLQEPGSGQAPKGSTITLTIV"
     gene            20826..21746
                     /locus_tag="PL014_02065"
     CDS             20826..21746
                     /locus_tag="PL014_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT55909.1"
                     /translation="MTAPAGRTRLATLLLVGVTAVWGSTFFLIRDLVETVPPADFLTV
                     RFGIAAIVMFALFRRQTLALSRRDLRIGVVLGVLYGSAQVLQTMGLQHTDASVSGFIT
                     GTYVVLTPVLGAVLLREHIRPVTWAAVALATAGLAVLSLQGFAVGAGEALTLFSAVLY
                     AGHIVALGRWSTAQAAVGLATVQAGVITVVTGVAAVPGGLTLPADGGQWASLLYMALI
                     AGAGALWAQTWAQAHLTATRAAIVMALEPVFAAFFAVLLGGESLTRRMLIGGAMVVTA
                     MYLVELKSTPRDPDATAAEDPPVEALHHDV"
     gene            complement(21837..22883)
                     /locus_tag="PL014_02070"
     CDS             complement(21837..22883)
                     /locus_tag="PL014_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOT55910.1"
                     /translation="MRADLLSDTLTRPTPAMRQAMAHAEVGDDVFGEDPTVRALEDKV
                     AALLGHEAGLFAPTGSLANQLGLRLHVGPGQELVADQLAHVLRAELGAAAAFSGITSR
                     SWPSERGLLDPAGPMSLVTTGVGPYQVETSLVVLENTHNFGGGTVQPIEAIREVRAAT
                     QPLGVAMHLDGARLWNAHVATGVPLTAYGEQFDTVSVCLSKGLGAPVGSVLVGSAEHM
                     AQARIWRKRYGAGMRQVGILAAAGIHALDHHLDRLDDDHARAARFAAACAEAAPASVD
                     AATVETNIVVLDVGALGWRAPAFVAALIEQGVRTYAVGPGRVRLVWHLDVDDAGTDAA
                     IDAATTLLRTQIDQ"
     gene            complement(22889..24241)
                     /locus_tag="PL014_02075"
     CDS             complement(22889..24241)
                     /locus_tag="PL014_02075"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HOT55911.1"
                     /translation="MSTTTAPDPRQRLAAGAGLPAAQQPVWPDADALAEAVATLASYP
                     PLVFAGECDVLRERMAAVARGEAFVLQGGDCAETFASATADNIRDRVKTILQMAAVLT
                     YGASVPVVKVGRMAGQYAKPRSSNFETRAGVTLPAFRGDMVNDFDFTPQSRTPDPQRL
                     VRAYHASSATLNLVRAFTTGGFADLRHVHDWNKGFVANAANARYERLAKDIDKAMKFM
                     NACGADFEAMRTTEFFSAHEALVLDYERPLTRVDSRTGELYDTSGHFVWVGERTRDLD
                     GAHIDFVASVRNPVGIKVSANADTDDLLRIIDKVDPQREPGRLTFITRMGSGRIREAL
                     PTIVEKVTASGASITWVCDPMHGNTFESASGYKTRDFDDVVEEVEGFFEVHRGLGTVP
                     GGIHVELTGNDVTECIGGSEKILDADLNLRYETVCDPRLNHQQSLELAFLVAEMLNQD
                     "
     assembly_gap    24497..24596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24693..25475)
                     /locus_tag="PL014_02080"
     CDS             complement(24693..25475)
                     /locus_tag="PL014_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT55912.1"
                     /translation="MTTTPPDAGGSPSASAPGDAAPTVLVADDHPLWLSALERDLTER
                     GLRIVATATDGPSTVRRARATRPDVLVLDLNLPGMRGDEVCHALGDLPTRVLILSASG
                     EQRDVLAAIKAGATGYLVKSASAQDIVDAVHATARGEAVFTPGLAGLVLGEFRRMADG
                     SASATATASEVSGTGATPAGAAAEERRRPIPELTERETEVLRLVATGMSYKEIAAELF
                     LSHRTVQNHVQNTLGKLHLHNRVELVRFALARGLEDEGPAQD"
     gene            complement(25472..26602)
                     /locus_tag="PL014_02085"
     CDS             complement(25472..26602)
                     /locus_tag="PL014_02085"
                     /inference="COORDINATES: protein motif:HMM:NF039922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5931 domain-containing protein"
                     /protein_id="HOT55913.1"
                     /translation="MESFHRGLDVYRPLAALYAAWLAWQRHETMVRPWVAVVVLAVML
                     AWSVGLLFYRRRTIGVVVVEVSIAVAGVLATLLAETPESIAAGQFTLPTIWAAGSVAG
                     GAVVAGARGGLIAAAVIAIVDLIEVGRPTQTTIHNIVLLVLLGGLIGLAVDLARRGLE
                     GEEQVLLERERLHERERIARVVHDGVLQSLAYIHRRGEDIGGEAADLAAIAAEQERSL
                     RRFVSGTGAPDAGGAVADLRAAHGEETDLRIALTARERPDVTVAVPADPVLVDVLVAH
                     EVDAALAAALDNVDRHAGPGARAWVLLEGDSSGLEVTIRDDGVGAQLADVFDAADRGR
                     LGVSSSIRGRIEDLGGTVTWTTRPGGGCVVRMTIPRTVKERA"
     gene            complement(26670..27476)
                     /locus_tag="PL014_02090"
     CDS             complement(26670..27476)
                     /locus_tag="PL014_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT55914.1"
                     /translation="MFYWVLKTVILGPILRLLFRPWVEGSENIPETGAAIFASNHLSF
                     SDSIFLPLCVPRRMTFLAKSDYFTGEGIKGRLTAAFFKGVGQLPVDRSGGRASEAALR
                     SGLKVLRRGELLGLYPEGTRSPDGRLYKGKTGVARMALEAGVPVIPVAMIDTDKAQPT
                     GKKIPKLIRVGVRIGEPLDFSRYEGMEGDRFVLRSITDEIMYAMMELSGQEYVDMYAT
                     AMKDRIAREKKSKAKEAQQAAQPGRAVSELEGVLDAEGLDDDSGPQRRAS"
     gene            27593..>28173
                     /locus_tag="PL014_02095"
     CDS             27593..>28173
                     /locus_tag="PL014_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55915.1"
                     /translation="MPDRPGDMDERDVDARFASIVAQWEHSAVDEPDAVAPAGAPVTD
                     DSPADPAAGPESAAGSASSSKSAVPGDSADRTPREADPSESAGPEAAAPAPRDPWVNP
                     SPLNLVVPASAWRTPDPQDAATERAAADADEALDAAIAAEEPHFEPPEVTLPPQEDLG
                     FWGAVIGLVAGPLMLLYVVFARPFHSTRWFVASV"
BASE COUNT         3903 a        10125 c        10045 g         3900 t
ORIGIN      
        1 agcagcaggg tgcggatgtg ggcgccgtcg acgtcggcgt cggtcatgat gatgaccttg
       61 ccgtagcggg ccgactcgag atcgaaggtg cggccagacc cggcgccgat gacctggatg
      121 atcgccgcgc actcggcgtt gccgagcatg tcggtgaccg acgccttctg gacgttgagg
      181 atcttgcccc ggatcggcag cagcgcctgg tggtcgctgt tgcgcgccag cttggccgtg
      241 ccgagagcgc tgtcgccctc gacgatgaac agctcggtgc gctcgacgtc ggtgctgcgg
      301 cagtcgacga gcttggcggg cagggtcgag gactccaggg cggtcttgcg gcgctgggtc
      361 tccttgtgca gccgcgcgct gatccgcgac ttcatctcgg cggtgacctt ctcgagcagc
      421 agggcagcct gctgcttctc gccgcgcctg gtcgacagga gccgctcggt gagctccttc
      481 tcgacgaccc gggcgacgat ggcgcgcacg gcgttggtgc cgaggacctc cttggtctgg
      541 ccctcgaact gcggctcggc caggcggacg gtgaccaccg cggtcagccc ggcgagcgcg
      601 tcgtccttct cgagcttgtc gttgccgacc ttcaggcgcc gggcattggc ctcgagctgc
      661 ttgcgcagca ccttcagcag ggcctgctcg aatccggcca ggtgggtgcc acccttgggg
      721 gtggcgatga tgttcacgaa cgaggagacc ttggtgtcgt acccggtacc ccaccgcacc
      781 gcaacgtcga cgccgcactc acgctcgacc tccgtggggc tcatgtgccc gttggcatcg
      841 aggaccggca cggtctcggt gaaggtgcca ctgccgtgca accgccacac gtcggtgacg
      901 gccgggtcgg gagcgaggta ctcgcagaac tcggagatgc cgccgtcgtg gacgaagacc
      961 tcctcacgca cctcgtcgcc gcgctggtcg ctgatgacga tcgtcagccc ggggacgagg
     1021 aaggaggtct gacgggcgcg ggcgagcagc ccttcgaggt cgaacgtcgc gtccttgagg
     1081 aagatctggc tgtcggccca gtaccgcacc cgcgttccgg acaccccacg gcgggtccta
     1141 ccgatgacct cgagctcgct gatgtcgacg tagggcgtga actcgtggga cgggtccttg
     1201 gcgcccttcg cgccggcgac gtcggagaac accccgggct ccccccgtcg gaagctcatc
     1261 gcataggtct tgcccccgcg gtcgacctcg acgtccaggc gggaggacag cgcgttgacg
     1321 accgacgcgc cgaccccgtg cagaccaccg gatgccgtgt acgagccgcc gccgaacttg
     1381 ccgccggcgt gcaggcgcgt gtagaccagc tcgacgccgg tgagcccggt gcgcggctcg
     1441 atgtccacgg gcacgccacg gccgttgtca cgcacctcga ccgagccgtc gccgtgcagg
     1501 gtgacctcca cgcggtcaca gtggccaccc agggcctcgt cgacggcgtt gtcgatgatc
     1561 tcccacaggc agtgcatgag cccgcgcgag tcggtggagc cgatgtacat gcctggacgc
     1621 ttgcgcaccg cctcgagacc ctcgaggacc tggaggtggt gggcggtgta ctcggacgct
     1681 gacttgctcg tcgcggcctt cttggcagca gtgggggaca cgaaatgcag ggtagtcgcc
     1741 gcctgcgaca ggctcgttca ggcgcaccgc gaggtggcgt cggatgccgt ggcaccggtc
     1801 gcgctccccg tcacggggcg cgtcgggaag gggtctggcc cggcacgctg cagatcacga
     1861 tccggacatg accggggcca cacggccgca ccctgcacag cgggaagcat ccgacgtgct
     1921 tgactgttgc aacgcacgcc gaccaccccg gacggcaacc acgcagaccc gagcgagctg
     1981 aggagccgac gtgaacgccg cactggcacc gaccctgaac gctgcggacc gctgcgaccg
     2041 ctgcagcgcc caggcatacg tccgagcgac cctccacgcc gggggtgaac tgctcttctg
     2101 tgcccaccac ggccgcgagc acatcccggc cctggcnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnggac tcgtcgacga gatcggtctg gtcatcccac cgctgctcat
     2281 catcgtggga ctgctcggca tcgtcgtgcc cgtgctgccg gggctgctgc tcatcctcgg
     2341 cggggtgctg ttgtgggccg ccctcgaggg tggcacgacc gcttggctga tcttggccgc
     2401 gagcctgctc gtcgccgtga tcgggtgggt gcttcagtac acgttgcccg gcaagcggat
     2461 gcgtgatcgg ggtgtgagca actccaccct ggtcatcgcc gtcatggtcg gcatcgtcgg
     2521 cttcttcgtg gtgccggtcg tcggcgcgat catcggcttc gtgctggcga tcttcgtcgt
     2581 cgagtacctg cgcagcaagg acaccgcgct ggcctgggag cggaccaagc acgccctcgt
     2641 cgcggtgctg cacagcatgg ggatcgaact cgccgcgggg ctcacgatcg cagccctgtt
     2701 cgtcggtggg ctcgtcgcga cctgagtcgt cgcatcgcac tcgaccctgc gtcacatcgc
     2761 actcgacaca gcgtgcgacg cagcgggccc gtcacggacc tgggtcgcag cctcaccagt
     2821 tgttctggcg cttctcccag cggtcatcga gccggtccat gaacgacttc tgacccttct
     2881 tgcggccccc tgacgagtgc ggtgccgtgc tgccgtcacc gcggacgaca cccagtgcgg
     2941 cccgacgcgg gggcgtggcg gcgaagaccg ccgcggtcac catgacggcg aacccgatgg
     3001 cacccaccca catggtggtg ttgacgccga cgaggaccag ggccaacccg agcacgatgc
     3061 cgacggcgcc gatcagccag cgtcgccgct gcatggacgc caaggaactt ccctgcatct
     3121 gcgaggcgaa cttggggtcc tcggcagcga gggcttgttc catctgttcg agcatcttct
     3181 gctcgtggtc tgagaggggc acggatcctc ccgtggtcgg cccgggcatc cggcggacgc
     3241 cctgttgtcc cttgagtcct caggataggt cccgatgtct ccccgcggaa gtcggtgtat
     3301 cacgttcgat gagactggcc ggtcgaaccg agcccgaccc gaaccgcgag cgggcccggt
     3361 caaccgcccg atcggcatcc cgccagccgt gctcgggctc cccgatcacc ccctggatgg
     3421 gtgacgccga ggcctccatg agcttctcca tgcgcactcc gacgagcctg atccgggccc
     3481 gctggagccc cagggcgtcg aacagcgcca ccgccccttc gtggatggcc cgcccgctgt
     3541 ccgtcggttc acgcagcgtc ttcgaccggg tgatcgtcgt aaaatcactg aacctcaccc
     3601 gcagggtgag ggttcgaccg gtcaacccgg cagcccgcat ccgggacgcg gtgcgctcgc
     3661 tgagggccag caggcgccgg tggatctcgg cggagtcgtc gatgtcgtgc tcgaaggtct
     3721 ggtcgctgcc gatgctcttc tcccgctgca cgtgctcgac cgatcgcggg tcgcgccccc
     3781 aggacaggtc gtgcaggtgg cgtccggtgg cctcgccgag gccgcgcacg agcgttgcga
     3841 gcggggtgtg ggcgatgtcg gcgacggtgc gcaggcccag gcgcagcaac gcgtcctcgg
     3901 tgcgctcccc cacaccccac agggcaccga ccggcagctg ctggacgaac ggcacgacct
     3961 cgtcgcgcgg cacgacgacc atcccgtcgg gcttggccag tccggaggcg atcttcgcga
     4021 cgaacttcgt cgaggccaca ccgaccgagc aggtgatgcc ctgctcgtcg tgcacggtgt
     4081 cgcggatgtg ctgaccgatg gtcgccgggc tgcccatggc ccgaaccgcc cccgagacgt
     4141 cgaggaaggc ctcgtcgagc gagagcggct cgacgaccgg cgtcaccgaa cggaaggtct
     4201 ccatgacggc tgcggagatc gcgctgtagc gggcgtggtc ggggggcagg acgaccgctt
     4261 gggggcagag ccgtcgcgcc cgcgacatcg gcatcgccga ggtcaccccg aaccggcgcg
     4321 cctcgtaggt ggccgagagc accacccctc ggttgccccc gccgatgatg accggggtgc
     4381 cgacgagctc ggggtgggag agcagggtcg ccgccgcgta gaaggcgtcc atgtcgacgt
     4441 gcaggacggt gcaccccgtg tcgtcgggcg gctcgctggc gtcccggtgc gggagggcgt
     4501 actggcgtcg gctcatcggt ggcctccggt ggcgtcagcc ccgggtggcg aggacgtgcc
     4561 ggacgctgcc gaggtcaccc agcagcgggt ggccgaggtg gcggctcgcg acctcctcga
     4621 gctcgagcag cgcagcccgg tcggcctcgg agtcgaccag cgcggaaggg acgaggtcgc
     4681 tgaagatgcg caccccgtga ctgtcgaccg tggtgaaccc gtgggtgcgc agcaggtcga
     4741 ggacgacgtt ctcgtcgaac cggcgtggtg ccgggtcgtc gtcaccccag cgcccgtcag
     4801 gccgctcgag gatggcctgc gcccgcgcga actgccccgc gagggcccgg gccagtgcgg
     4861 caggcagccg ctgggcgacg acgagggaga gggtgcctcc gggtgcgagc acggtcgcga
     4921 tgcccgccat ggcggcatcc gggtcgtcga cgacctcgag ggtgccgtgg aggcagacga
     4981 ggtcgtatgc cgccggctcg ccgtccgcgg cgagctcgcc gcccacccca agatcgcgca
     5041 gggtgtcggt gtcgccctgc acggggtgga tccgccgcga ggtgtcggtc tcggcggcgc
     5101 ggcggcgcag cgaggcgagg gcgtcggggc tggggtcgac gacggtgacg tcgtgcccgg
     5161 cgacggcgag gggcacggcg gtgccgccgg tgccgccgcc gaggtcgagc acgcgcaggg
     5221 gacgcccgag ctcggcgctg cgccgggcag cgaaggcgtc gagggcgccg tggacggcag
     5281 ccgtgcgcag cccgggaggg cctcctgccc gacgggatcg ggcccgctcg tcagccacgg
     5341 ctggtctcct cgctcaggtc ggtaccgcag ggtcggtgtc tggcgtccca ccctagtgtc
     5401 cggaactgcc ggggctggcg tgccacagct tgcgcggggg cagcgcgtcg accggtgcgc
     5461 cccgccccga gcgcgggtcc agccccggtg ggcgggtgtc ggcgtagggc gactggcgaa
     5521 agcccgaggc gtgcacgagg acgcgccgcc cggtgcgggc gtcctgctcg atcgcggcga
     5581 gccgggcggc ctcggcgcgt gccacggcat ccgcctcccc cgccgcgacg acggcctgca
     5641 cccgctcgag gccaccgagc tgccaggcgt cccagaggcc ggcgagctcc caggccccgg
     5701 tggcgcggat ggagacaccg cgatccccgg tgcgtcgcag caccccgcgc acgaggagca
     5761 gccacgagtg gaagacgacg tcggcgtagg ggccctggac gtcctcgaag aaggtgcagt
     5821 cggctggccc ggtggagtcg tcgagggtga ggaagaccac ccgtcgcccc gagcgcaccg
     5881 gcggggtctg ggtggcgacc ttgaccccgg ccacccacac ccggctgcgg ctgcgggtgg
     5941 cgagcaggtc gacgctgcgg gtgacccgaa gcgcccgcag cagcgggtcg tagaactgca
     6001 cgacgtgggc ggacacgtcg agcccgagca cgtcgagctc ggcacgcacc ttctcggggc
     6061 cggtcagctc gggcagcccg gctccgggga ccagccgggg ccggtcaccg aggtcgaggg
     6121 tgagctgggt cggttgggca gccgccggga cgacctcgcg cgccgcctgc gactgggcgg
     6181 ccgcctgctc ccggacgtcg agcgggagcc cggccagcgc agccgcgccg tcctcgcgcg
     6241 gcggggcgat gctcggcacc gcgaggcgtc ggctgcgcct cgccggcccc ccggagccgc
     6301 ggtcccaccg ctcgagctcg gcgacgtgca gcagcaggtc gcggcgggtg acccgaccac
     6361 gccggccgag cccaccgtcc cccatcccgt gcagggagtc gaacgcccct gcgaggacga
     6421 gccgctcgac gaccgggcga ctcacccggg cccgcgtcca gaagtcaccg aggtcggcgt
     6481 acggctggcc cgcgacgacc cgggcgacct cggcggcgct gatccccttg acgtcggcga
     6541 gggagaggcg gatgccgtac ggctggttcg cgcgctgcca cgctggctcg acggcatcca
     6601 gcaccccggc atacggcacc ccggcatccg gctccacggc atccggggtg ggctctgaca
     6661 cctgctcgat gcggtaggtg tcggtggagg cgttgacgtc gaggccgagg atcgggatgc
     6721 cgaaggatcg ggcgtcgtcg agcagcagcc gcttggggta catgccgggg tcgtgggtga
     6781 ggatgccggc gaggaaggcc gccgggtggt gggtcttcag ccacgccgag tggaaggtcg
     6841 gcagggcgaa ggccgcggcg tgggccttgc agaacccgaa gctcgcgaac gacttcagca
     6901 cctcccagat gcggtcgcgg tcctcagcgg tgtagccccg ggcggcggcc gcggggcgcc
     6961 accacgcctc gacgtcgacc tggccctgcg gggtgcccat gctgcggcgc acctcgtcgg
     7021 cctgggccag gctcaccccg gtcgtctcgg cgacgatgac cagcacctgc tcgtggaaga
     7081 cgacgacgcc ctcggtctcc tccagcgccg ggatcaggct ggggtggatg aaccggcgcg
     7141 tgctccaccc gtggcggctg ttgaggaacg gggtgatcat gtcggacttg accgggcccg
     7201 gccggaacag cgagatgtcg acgatgaggt cgtcgaaccg gcgcggcccg agcttgccga
     7261 tgagctcgcg ctgccccggg ctctcgatct ggaacatccc cagggtgcgg gtggtgcgga
     7321 tcagctcgta ggtggctggg tcctcgtggg gcacctgctc ctcgtcgtcg aggtcgatgt
     7381 gctccccgct cacccgctcg acctcggcga ccgcgtgggc catcgccgac tgcatgcgga
     7441 tgccgagcac gtcgagcttg aggaagccca gggtctcgac gtcgtccttg tcgaactggc
     7501 tcatcgggaa ccccagccag ctcgcctcga ccggggtgcg gtcgagcagg cccgagttgg
     7561 acagcacgat gccgcagggg tgcagcgcga tgtggcgcgg gagaccgtcg agggcctcga
     7621 cgaggtcgaa gaaggccgcc atgcgagggc tgtcgaggcc gcggctgcgc agttcgggca
     7681 ggtcggcgat ggcgtggcgg gcgtccttgg cgcggatgtg cgggaacgcc ttggcgatct
     7741 cgtcgacctc ggtggggggc agcccgagcg cggcgccgac gtcgcgcacg gcgtggcgca
     7801 ccttgtaggt gtccatcatc gacacacacg tcacccgctc accgccgaac cgggtgagca
     7861 cggcctcgta gatctcggtg cgccgggccg actcgacgtc gacgtcgatg tcgggcagtt
     7921 cggcgcgcag cggggagcag aaccgctcca tgagcagccg gtggcggatc gggtcgaccc
     7981 cgctgatgcc caggaggtgg ttgaccaggc tgccggcgcc cgagccgcgg gcggccaccc
     8041 gcaccccgcg ggcccggatg aggtcggtga cctcggcgac ggtgaggaag tacgtggggt
     8101 agcccagggt ctcgatgacg gccagttcct cgtcgagccg gtcgagcacc acctgtcgct
     8161 cggcctcccc ggcccccggg tagcggcggg cgaccccgtc gcgacagcgc tgcgcgagca
     8221 ccgccatggg gtcgtcggcg gcgctgaggc cgagcacctg cgcctcgggc aggtgcaccg
     8281 ccccgatgcc gaggtcgggc cgggcctgct gggtgcactc ggccgcgacg agggcggtca
     8341 tcgcgacgag gcgatcggca cgggagcggt ctcccccggt gacctcgagg gcgttggcgc
     8401 gcatctgcgc ggtgctcgcg aggtgcccgg agtcggtgac ccggtcgagg tggcgcgagt
     8461 cgaggacgac gaggcggcgg gcggcgtcga gcaggtcgac ggtgcgcacc tggtcggggt
     8521 cgacgtggcg caccgcggcg gtgaggacgg cggggatgcc ggccgcgtcg gcgagggaga
     8581 gcaacctggc agcgtgcgac cggcatccgg aggttccttc cggaccaccg tgcagcacca
     8641 cctcgacggc gagaccgtca cggggcagca ccgtctgcca ggccgcgagc aggtgccggg
     8701 cgcggtctcg gcgacccgcg agggctgccc gcccgacgtc ggagtcgggg ccgagcagca
     8761 cgagcagccg actcggccca cctgcctccc cgacgtcgtg agcccaggtg gccagcagat
     8821 cacgagtggt caccgggacc ccccgctcgc cgcgcaggtg ggtctgcgtg acgagccggc
     8881 acagggcagc ccaccccgca ccgggcgcca ccccggcgac ctggccgcgg gcgagcacgc
     8941 tcacccgtgg gcggcggggg tcgacgatgg ccccaccccg cacgggggtg cgcgggcgct
     9001 gggggtgact cacggcatcc ggtctcgcac tgccgtccca ggtggaacgg ccagccgtga
     9061 cgccgccgcc cgaactggag cctccaaccg aaccaccggt gcccacgtcg acggcgaggt
     9121 cgaccccgag gaccgggtcg atgccggccc gcccgcacgc cgtggcgaac tgcaccgcgc
     9181 cgtagagccc gtcacggtcg gtgagggcca gcgcaccctg gccgtgggcg acggcgcgct
     9241 cgacgagctc ctcgggcatc gaggcgccgt agcgcatcga gaaccccgag gcgacgtgga
     9301 ggtgggcgaa cccgtccacg acgcactcag gccgagcgca cgggcacgag ccgctgcggg
     9361 cgctgcaccc cgggcacccc gaggcgacgg gccgcgaggt cgacgaaggt ctcggcgtcc
     9421 cgcagcaggt cgtcggcctc gcgggcgctg acccgcacga gcccggtctc gaccccgacc
     9481 cgctgccccg tggacgccgc gaagcgctgg gcccactcgg tgagctccgg ggcgacccgg
     9541 gcggtgagcg cccacacgtc gtggggtccg ttgccgcgag cggccaaacc ggcaccgggg
     9601 tgggtcgccg cccgggctgc gacgatcgcc gccccggcgc gcagggccgc gagcgaggcg
     9661 gcgaggtagc gcgccgatgc cgccgaggcg gcgtacccgc tggtcaggga ctctcgggac
     9721 cggtcgagga ggtccagggt ggtcgcggcg tggctcatcg ggggctcctc gagatggttc
     9781 atcggatgct gcgtgctcgg tgcggtgtcg atcggtgcgg tgtcgatcgg tgcggtgtcc
     9841 atggccggtg ggactcagtc gtgggcgcgc aggagcagcc agcggtcgtc gtcgagcccg
     9901 aggtcgaaga ccccggcggt gccggacggc gggcgcgcct cgacccgcca gacccggcgc
     9961 tgcgggctcg gcaacgcggc cccaccggtg aggtcaccga cctcgcgcca ccaggcccga
    10021 cgctcctgcc acgaggccag cacggcgcac acggtgtagc gccgaccgcg ccagacgaag
    10081 tcgcggggac cgtcagcgcc gacccggggt tcgacacgag gttcgccgag ggggtcgccg
    10141 ccaacaccca gatcgctgct gcttcggacg ctgatgtgct cgtcgtgacg gcggaccatg
    10201 acctcccctt tcccgcgctc gctcgtggtt caccagcgac cgccgggcct cggcgatgcc
    10261 ggtcaatgat cgaacagatg ttcgatgtct ccaccataac gcgacccacc gacaggtcgt
    10321 caagcccgca tcgagcccgc gtcaccgggt cgctacgctg ccgccatgac ccagccgccg
    10381 agcgagcaga accccccgcg agacctctcc gagcacgccg gtgtgcgctc ggtgatgcag
    10441 gccctggccg agcacggtct cagtccgcag gtgcgccacc tccccgatgc cgtgcgcacc
    10501 gcgcaggccg ccgccgacgc cctgggcatc tcgcctgccg agatcgcgaa cagcctcgtc
    10561 ttcgtcgcga cgacccacga cggcgagcgc ctccccctgc tcgtcatggc gtcgggaggg
    10621 caccgggtcg acgtcgtctc cgtcgcggag cgggcaggtc tcgcgagcat cgagcgggcc
    10681 gaccccgcct tcgtgcgcga gcacaccggc ttcgcgatcg gcggcgtcgc accggtcggc
    10741 cacccgcacc cgctgcgcac gatcatcgac acccacctcg cgacgtaccc cgtcgtctgg
    10801 gccgccgcag gccacagcca cagcgtcttc gcgacgtcgt acgacgacct ggtgcggatc
    10861 accggcggtc agccgatgga cgtcgcctga ccgagccgaa gaccggctga ttgaacccgg
    10921 atccccggaa ccagaccccg acctgccaca tctgagtacg agtacgcatg cggcaggccc
    10981 gtgctggccg tcatgctcga cacagtggtc gcgccgaccg cgggagtgga cgttgagggg
    11041 ggtccgtgcc cgcggtcggc gcgaccgacc catgcggggc tcggccggct cgacaaatcg
    11101 gcgtgcgagc acgaaggtcg tcggcaacac tgacgcggtg tacctgacca tctcgacgac
    11161 ccaccgtccc gcgacggacc tcgggtacct gctgcacaag caccccgacc gggtccagca
    11221 gttcacccag agcttcgggg tcgcgacggt gttctacccg caggccaccg acgagcgctg
    11281 cacggccgcc ctgctcctcg agatcgaccc ggtgcgactg gcgcgcggtc gcgcccgcaa
    11341 cctgcccgac ttcagcctcg gccagtacgt caacgaccgt tcctacgccg cgtcctccct
    11401 gctcgccgtg accctggccg aggtgttcag caccgcccgg atcgggcgct gcgcgtcgca
    11461 ccaggacctg gccgactccc ccatccccct gcagatccgg atccccgtgc tgccgtgccg
    11521 gggcggccct gccatcgccc accggatctt cgagccgctg ggctggcagg tcgtcgccga
    11581 ccccatcgac ctcgacgagg cattcccgca gtggggcgcc tcccgttacg tcgacctcac
    11641 cctcaccggc accgtccgcc tggccgatgc cctcacccag ctgcacgtgc tgctgccggt
    11701 gctcgatgag tccaagcact actggcaggg cccggacgag gtcgacaagc tcctgcgctc
    11761 cggcggcgac tggctcgcgg gccaccccga ggccgagctc atcacccggc gctacctcag
    11821 ccgcaccggc tacacccggg tggcgctcga acgcctcgcc gagctcggcg acgacgctcc
    11881 cactgacgac cccgcggaag actggtgcgc gacaccgaca tcggatgccg tggagccggt
    11941 cgcctcgccc gtcaccaccg agcccctcac caccgcgcgc acccccttga accgccaacg
    12001 gcacgatgcc gtgctcgccg agctgctcaa cctcggcgtg agctcggtga tcgacctcgg
    12061 ctgcggcccc ggggccttcc tcgagcgcct cgtgcgcacc ccctccttca ccaaggtggc
    12121 cggcagcgac gtgtcgacga ggtcgttgca ccaggctgcc cgccgcctgc acctcgacac
    12181 gatgaccgag cgccaggccg accgggtcga gctcttccag ggcgccctga cctacgagga
    12241 cccgcgctac gccgggttcg acgccgccgt gctcatggag gtggtcgaac acgtcgaccc
    12301 gagccgcctg cccgccctgg agtcggtcgt cttcggcacc gcctcccccg gtgccgtgct
    12361 cgtcaccaca cccaaccggg agcacaacgt ccactacgac ggcctgaccg gcatgcggca
    12421 tcccgaccac cgcttcgagt gggacaggga cgagttcgcg acctggtgcg cgcgcgtggc
    12481 ggccacccac gggtactcgg tggtgatccg cggcatcggc gatcccgagg ccgccaccgg
    12541 ccacccgacc cagctggggg tgttcacccg tgacgcctga gacgagcgac acgaccgagg
    12601 tgcgcgtccc ggcatccggg ctcgtcgtgc tcgtcggagt ctccggaagc ggcaagtcga
    12661 gcttcgcccg cgagcacttc cgccccaccg agatcgtgtc gagcgatgcc tgccgggcga
    12721 tggtgtccga cgacgagaac gaccagtccg cgacaccgga cgccttcgag ctgctcaacc
    12781 acatcgtcgg cacgaggctg cgccgtgggc tgctcacggt cgtcgacgcc acgaacgtgc
    12841 agtccaaggc ccgggccgag ctcgtcagcc tggcccgccg gcaccacgcc ctcgtcgacg
    12901 ccatcgtgct cgaggtgccc gagagcgtcg ccgggcggcg caacgccggg cgtgccgacc
    12961 gcgacgtcgg gatgccggtc gtgcggcgcc agcagcggga cctcagcagg tcgaagaagc
    13021 gcctgcgcaa ggaagggttc cggcaggtgc acgtgctcga cgccggcggc gcaccacctc
    13081 gggtgcagat cgtgcgcgag cgcccgtgga acgaccggag cgaggtgcac ggcccgttcg
    13141 acatcatcgg cgacgtgcac ggctgcctcg gtgagctgcg caccctgctc gaggacctcg
    13201 ggtgggccct cgatttcgac gatgccggtc gtgccgtcgg tgcttgccac ccgcagggtc
    13261 ggctcgccgt gttcgtcggc gacctcgtcg accgcgggcc ggactccccc ggcgtcgtgc
    13321 ggctcgtcat gggcatggtc gagcagggca gcgccctgtg tgtctcgggc aaccacgagg
    13381 ccaagctcgt gcgggcgctg cgcggcacca cggtgacggt ctcgcacggg ttggcgcagt
    13441 cgctcgagca gttcggccgc gaacccgacc agttccgcgc cgacgcgctg gcgttcatgg
    13501 acgggctcgt cgcgcactac gtcctcgacg acggccgcct cgtcgtcgcc cacgccggcc
    13561 tgaaggagga gtaccacggg cggacctccg cccgggtgcg ggccttcgcg ctgtacggcg
    13621 acacctcggg cgagaccgac gagtacggcc tgccggtgcg atacccctgg gcgcaggact
    13681 accggggcaa ggccatggtg gtctacgggc acacccccgt gccgagcgcg cagtggctga
    13741 acaacaccat ctgcctggac accggcgcgg tgttcggtgg cgcgctcacc gcgctgcgct
    13801 accccgagcg ccagatcgtc tcggtgcctg cgcagcagca gtggtacgaa ccggtccggc
    13861 cgctcggtgt gccgagcccc gaccggcagc cgtcggtgct gcgcctcgac gacgtctccg
    13921 ggacgcgctg gctcgacagc acccgagccg gtcgggtcaa ggtcgaggag gagaacgcag
    13981 cggctgcgct cgaggtgatg agccggttcg ccgtcgaccc ccgctggctg gtgtacctgc
    14041 cgccgacgat ctcaccggcg cgcgcctcca cccgcgaggg ctacctcgag cacccgcagg
    14101 aggccttcga cgagtacgcc gggtgggggg tcacctcggt ggtgtgcgag gagaagcaca
    14161 tgggctcgcg cgcgatcgcc gtgctcaccc gcgacgccgc ggtcgcggcc cggcgcttcg
    14221 gcatcgacga cgggagcagt ggctgcgtct acacccgcac gggtcggccg ttcttctccg
    14281 acaccgcgcc cctcgtcgag gcgatgcggg atgccgtcgc gcccctgttc gcgtcgctgg
    14341 acaccgactg ggtcgccctg gactgcgagc tgctgccctg gtcggccaag gccctggacc
    14401 tcatcaccga ccagtacgcc tcggtcgggg cggccgcccg gcacgcgctg ccggccgcgc
    14461 tcgacggtct cgacgcggca tccggtcgcg gcctggacgt cgaggacctg cggcggcgca
    14521 ccgcggaccg gcacaccaac gcgctcgcct tccgcgaggc ctacgcggcg tacgtgcgcc
    14581 cgaccgacgg gctggagggg gtcaccctgg cccccttcca ggtgctcgcc gtcgaggggc
    14641 gcctgctcgc cgagacccac ccgcacccgt ggcacctcgc ccagctcgcc gggctcgaca
    14701 gcgacctcat caccccgacg cggcaccgga tcgtcgacct gaccgcggac cgggagcgcg
    14761 aggatgccgt gcgctggtgg gaggagctca cggccgccgg tggggagggc atggtcgtca
    14821 agccggccca cgtcgtcacg gggagggccc agccggggct gaaggtgcgt ggccgcgagt
    14881 acctgcggat catctacggc cccgactaca ccgatgccct gccgctgctg cgcgagcgca
    14941 acctcaccca caagaggcgc ctggcgctgc gcgagcacgg gatggggctc gacgcgctgg
    15001 cggggttcgt cgccggtgac ccgctctggc aggtgcacca gcgggtgttc gccgtgctcg
    15061 cgctggagtc ggagccggtc gacccccggc tgtgagcggg ggtgggcgcg gcctcagagg
    15121 ctgaggttct cgtagacctc gagggtcgcc gtcgatcggt tcatcgtgat gaagtgcagg
    15181 ccgggcgcgc cctcgtcgag catccgctgg ccgatctcgg tggcgatctc gacgccgacg
    15241 gcccgcacgg cggccgggtc gtccttgact gcctcgagcc gctcgacgac cgcaggcggc
    15301 agcggcgtgc ccatgagctc ggacatccgc tggatctgct tgaggttggt gaccggcatc
    15361 agaccggggg tgatcggcag gtcgcggcgg gcggcgacga ggtcacggaa gcgcaggtag
    15421 ctgtcggcgt cgaacaccat ctgggtcacc gcgaagctcg ccccggcatc cgccttgcgc
    15481 acgagcacgt cgacgtcgtg ccccaggtcg gggctgtccg ggtgcacgtc agggaaggcg
    15541 gcgacgccca ccgtgaagtc gccgaggccg cggatgaggg cgacgagctg gtcggcgtgg
    15601 tccagaccct cggggtgcgg ggtccactgg ccgcgcacgt tgccgggcgg gtcaccgcgc
    15661 agcgcgagga tgttgcggac ccctgatgcc gcgtacgccc cgacgacctg gcgcaccgcg
    15721 cgcttggacg ccccgacgca ggtgaggtgg gccaacggcg tgagggtcgt ctcgtcggcg
    15781 atgcgaccgg tgacgcgcac ggtgcggtcc tgggtcgtgc cgcccgcgcc gtaggtcacc
    15841 gagacgaagg ccggtcgcag tcgctcgagg tcgcggatgg ccgtccacag ctgggcctcg
    15901 gaggcgtcgt ccttgggtgg gaagaactcg aagctgatcg agggtcgagg ctgcgccaga
    15961 cggcccggaa tcgatgccac gaggtgcgcc ggccgccctc cgcctccgta gtgcgccatg
    16021 gcggtgaggg tagtccagcg cccacgccgt aggctctggg gacccagcat ctgaccatcg
    16081 gcgcgaggtg cgccgcccgg tcaaccccca ggaggaaccc cgtggccagc ccgctcgaca
    16141 ccgcagacct gcggcaccgg gtccaggact gcctcgacgc cgagctcacg acccaggccg
    16201 ccgtgctcgc cgagctcggg ccggacgtcg acgagctggt gacggccatc tcggcgctgc
    16261 ttcgcggggg caagcgcctg cgcgccgcct tcctctactg gggccaccgc gcggccggcc
    16321 gccccgactc cgacgccctc atccggctcg ccagctcgat ggagctcttc caggcggccg
    16381 cactcatcca cgacgacgtc atggacgact ccgacacccg tcgcgggatg ccggccgccc
    16441 accgtgcgtt cgcgagcgcc cacggcgggc gtggctgggg aggcgacagc gaccggttcg
    16501 gcctggccgg cgcggtgctc gccggaaacc tgtgcctgac gtggaccgac gaggtgtacg
    16561 ccacctgtgg actctcagag agtgacctcg cgcgcgggcg caccgtcttc gaccggatgc
    16621 gctcccagct catggccggg cagttcctcg acgtcgtgga gtcgatgcgc ccgtgggacg
    16681 ggctgcccgc ggccgagcgc atcgagcggg ccgaccgggt gattcgctac aagagcgcga
    16741 agtactccgt ggagcacccg ctgctcatcg gggccacgac gggtggcctg gacgacgcgg
    16801 gcgtggaggc gctgtcgcgc tacgggctcg acctcggccg ggccttccag ctgcgtgacg
    16861 acctgctcgg ggtcttcggc gacccgcagg agaccggcaa acccgccggt gacgacctgc
    16921 gcgagggcaa gcgcaccgtg ctgctcgccc atgcgctggc cggtctcgac gccggcggtc
    16981 aggcaagggt ggagtcgctc gtcggccgcg ccgacctgga cgcgtcgcag gtcgaggagt
    17041 tgcgcgccac gatcgtcggg tcgggtgcgg tggcgcggct cgaggacgag atcacccggc
    17101 tcgcggatgc cgcgacgagc gccctcgggg acgccacgtc gatcggtgac gaggctcgtg
    17161 cggccctgct cgacctcgtc ggcatcacca ccgcccgcac cgcctgaccc ccgttccgac
    17221 tgacccccgt tccgcctgac ccccgggccg ctatcgcgcg agctcgacgt cgatgtcgcg
    17281 tgcgagggtc gcgatcaccg gccaggcgcc ctcggagctg ttgccgagga cgctcacggt
    17341 cgtgcgcgac tcgacgaggt gggtcgaccg gaaggagacc cccgcgtcgt agccctcgat
    17401 gatcagggct gggtgcgagc ggtgcagcca gaaacccatc gcactgcgca tcccctcctc
    17461 gggcacgtcg tgccgagccg cggtcatccg ggcgagcgtg gccggcgaga cgatctgccc
    17521 cgccgtcagg gccccccaga accggtgcag gtcggtggcc gtggtgaagg cgccgccgtc
    17581 accggcggcg agcaccggca ggtgcaaggt gttgacgagg ttcccctcgg ggttcaggta
    17641 gcccagggcc acgtcgcccg gcaggtcgtt gaggggaagg aagccggtgt gctccaggcc
    17701 agcgggctcg agcacgaggc ggcgcacgac atcgtggtag ccctgcccgg tcgcccgctc
    17761 cagcacgacg gccaggacgg cgtagtcgct gttgcagtag gtgaaccgca gcccgggctc
    17821 acctcgttgc gcatgcccgt cgagcatcgg caggaaggcc tgcgccgtgg tgagggtgtg
    17881 caccggcagc gtcatgacga actgcgacgg ggaccagccg ccgtcctcgt cgaggtagtc
    17941 gccgatccct gaggtgtgct ccagcaggtg ctcgatcgtc acgccgtcgt cgacgagcgg
    18001 gaggtcgtcg ccgagccact cgcgcacccg gtcactgagc cccagcacac cctgctcgac
    18061 gaggcacatc acggccaggg cggtgaaagc cttgctgccg ctggcgatcc cgaagcgggc
    18121 atcggccgtc atcggcaccc ccagcgcccg gtgcacgaag ccctcgcacc gctcgaggac
    18181 acggcggtcg ccgacgtcga cggtcaccac cccggtgaac gcctgcgcgg ccaccgcgtc
    18241 atcgagggcc tgctcgtcga ccaccacgtg cggctccttc cggaaacccc catcgtggcg
    18301 cagccgctgc cccggcggaa gggtcagccc gcggtcagaa cgcttcttcc atggcgcggc
    18361 ggcgcacctc ggtcttgaag cccgcgtgga tggcgttgag cggcgtgccc ggcacgggaa
    18421 gcgtctcgtc gggcgtgtgc aaccagcgaa tgatctcgat gtcggtcatc ccgccgtccg
    18481 ccagcacggt gaacgtgccc ttgagggcgt ggtgcggccc ctcgtcggtg aggaagcccg
    18541 ccggaacggc cacgacccgc cgctccccga tgcggtggga gagcagctca cggtcctcga
    18601 tgagccggcg caccgtcgac aacggcagac cgaggcgctc ggcgatgtcc ggaacggtca
    18661 gccactcccc gaccagtgcc tcgaggtcag caccgggtgc ggctgctgcg gcgtcgttcg
    18721 tctcgctctg cggggtctcg ttcacggcgc ttagcgtgcc acagcacccg tgtccggcgg
    18781 gaggacacac ctgcgacggg gccctcagat gacggatcgc agcccggatc tacgtacact
    18841 cctacggtgt catccaacgg cgacgcgctc atcgggcgca ccctcgacgg gcgctatcga
    18901 gtgctccgtc gactcgccga cggcggaatg gccaccgtct acctggccct cgacgagcgc
    18961 ctcggtcgcg aggtggcgct gaaggtcatg cgtgagcacc tcgtccacga cgagagcttc
    19021 gtcacccgct tccaccgcga ggcgcgctcg gcggcatccc tgtcgcaccc caacgtcgtc
    19081 gccgtctacg accagggtcg cgacggtgac gtcatgttcc tcgtcatgga gcacgttcct
    19141 gggttgaccc tgcgtgaggt gatgcgcgag gagggggccc tcaccccgcg cgccgcgctc
    19201 gacatcctcg aaccggtgct gctcgccctc ggcgaggccc acgccaacgg cctgatccac
    19261 cgtgacgtca agcccgagaa cgtcatcatc aacgacaacg gcacggtcaa ggtcgccgac
    19321 ttcggtctgg cccgagccgt aaccagccag accgtcacga gcacgtccgg tgtgctgctc
    19381 ggcaccgtcg cctacctctc ccccgaacag gtggagcggg gcatcgctga tgcccgcagc
    19441 gacgtctacg ccgccgggct gatgctgttc gagatgctga ccggcaccaa ggccgtcacc
    19501 ggtgacacgg cgatccacgt cgcctaccag cacgtccacg gagggatgcc gttgccctcc
    19561 agccgggtgc ccggcctcga cccggcgctg gacgacctcg tcgccgccgc cacggcccgc
    19621 gaccccgacg accggcccgc ggatgccgcg gccctgctca ccatgctgcg gcgcacccgg
    19681 gccctgctgt cccccgcagc cctggactca cgacccgccg gcgccgaggc gctcgcggcc
    19741 gccgccctgc cgaccaccgc cgccgcctcc ccggccgcga ccgccgccct gccgatgccg
    19801 ggaagccact ccgcgacggc atccggcagc gtcacgaccc gcccgcacac cgacacccgg
    19861 gccctcaccc tgcccgcctc gcctccctct cctcctgccc cgctcgaggg cagggtggtc
    19921 gtcggtggag gccgatcggg tgggacgcga cgctggtggc cagccatcgc ggcatccgcc
    19981 ctgatcgccg ccctgacggc ctggttcttc atgctcggtc cgggcgcgat ggcgaccgtc
    20041 ccctcggtgc tgggtcagac cgaggccgcc gccgtggctg ccatcgacga cgcggcgctc
    20101 accccgaccg tgacgcaagc cttcgacgag gtcgtcccgg ccggccaggt gatctccacg
    20161 gacccgggcg caggcgagac gatccggcgc ggtgccgggg tctccgtcgt cgtgtcgaag
    20221 gggccggaac gctacgaggt gccgcccgtg gtcgggatga ccctcaccga ggcgacggcc
    20281 agcatcgccg aggtcaacct cgtcgtcggc gaggtcgagg aggtcttcga cgagaccgtg
    20341 cccaaggatg aggtcatctc ggccgacccc gggcccggtg ccagcctgaa gaagggcgtc
    20401 gaggtcaacc tcacggtgag caagggccgc aaacccatcg aggtgcgcga ctacaccggc
    20461 aaggacgccg ccgctgcgca gaagtcgctc accgacctcg ggctgaaggt cgacgccagc
    20521 aaggaagaga acagcgacac ggtcgccaag ggcatggtga tcagccagag cccgaacgac
    20581 ggcaccctgt tcaagggcga caccgtgacg ctggtcgtct ccaagggccc ggtcatggtc
    20641 gacgtcccca acgtggtcgg gcagcaggtc gaccaggccc gcgcggcgct ggaggccgcg
    20701 ggcttcaagg tcaccgttcg caaggccctc ggcgggttct tcggcaccgt gcgactgcag
    20761 gagccgggca gcggccaggc gcccaagggc agcaccatca ccctgacgat cgtctgagcg
    20821 gcgccatgac tgctcccgcc gggcggaccc ggctcgccac gctgctgctc gtcggcgtca
    20881 ccgccgtgtg gggctcgacg ttcttcctca tccgcgacct cgtcgagacc gtgcccccgg
    20941 ccgacttcct cacggtgcgc ttcggcatcg ccgccatcgt catgttcgcg ctgttccggc
    21001 gccagaccct ggcactcagt cgccgcgacc tgcgcatcgg ggtcgttctc ggggtgctgt
    21061 acgggtctgc gcaggtgctg cagacgatgg gcctgcagca caccgacgcg tcggtctcgg
    21121 gcttcatcac gggcacctac gtcgtgctga ccccggtgct cggggcggtg ctgctgcgcg
    21181 agcacatccg gccggtgacg tgggctgccg tcgccctggc cacggccggg ctggcggtgc
    21241 tgtcactcca ggggtttgcc gtcggggcgg gcgaggcgct gaccctgttc tccgccgttc
    21301 tctacgccgg ccacatcgtc gccctcggac ggtggtcgac ggcgcaggcc gcggtcggtc
    21361 tggccaccgt gcaggctggg gtgatcaccg tggtcacggg cgtcgccgcc gtcccgggcg
    21421 ggctgaccct gccggccgac ggagggcagt gggcatcgct gctgtacatg gcgctcatcg
    21481 ccggtgccgg tgcgctgtgg gcgcagacct gggcgcaggc ccacctcacg gcgacccggg
    21541 cggccatcgt catggccctg gagccggtgt tcgccgcgtt cttcgccgtg ctgctcggcg
    21601 gtgagagcct gacccgacgc atgttgatcg gcggcgccat ggtggtgacg gcgatgtacc
    21661 tcgtggagct gaagtcgacc ccgcgcgacc cggacgccac cgccgcggag gaccctccgg
    21721 tcgaagccct gcaccacgac gtctgaccga gcagactcga cgtaccgcgt acctcgagtg
    21781 acctattggt cgatctcggt acgccaacgc ccgtgcaggc tcgtgccccg agcgtcctat
    21841 tggtcgatct gggtacgcag gagggtcgtc gcggcgtcga tggcggcgtc cgtgcccgcg
    21901 tcgtcgacgt cgaggtgcca caccaagcgc acccgacccg gcccgacggc atacgtgcgc
    21961 acgccctgct cgatgagggc ggcgacgaac gccggtgccc gccagccgag cgcaccgacg
    22021 tcgagcacga cgatgttcgt ctcgacggtc gcggcgtcaa ccgacgcagg agccgcctcg
    22081 gcacacgccg cggcgaagcg cgcagcccgg gcgtggtcgt cgtcgagacg atcgaggtgg
    22141 tggtccaggg cgtggatgcc ggccgccgcc aggatgccga cctgccgcat ccccgccccg
    22201 taccgcttgc gccagatccg ggcctgcgcc atgtgctccg ccgagcccac gagcaccgag
    22261 cccaccgggg caccgagccc cttggacagg cacacgctga ccgtgtcgaa ctgctcgccg
    22321 tatgccgtga gcggcacccc ggtcgcgacg tgggcgttcc acagccgcgc cccgtcgagg
    22381 tgcatcgcca ccccgagggg ctgggtggct gcccgcacct cacggatggc ctcgatcggc
    22441 tgcaccgtgc cgccaccgaa gttgtgggtg ttctccagca cgacgagcga ggtctcgacc
    22501 tggtagggcc cgaccccggt cgtgacgagc gacatcggcc ccgccgggtc gagcagcccg
    22561 cgctcgctgg gccaggaccg gctcgtgatg cccgagaacg ccgccgccgc cccgagctcg
    22621 gcacgcagca cgtgcgccag ctggtcggcc acgagctcct ggccggggcc gacgtgcagg
    22681 cgcagcccga gctggttggc cagggagccg gtcggtgcga acagcccggc ctcgtgaccg
    22741 agcagcgcgg cgaccttgtc ctccagcgcc cggaccgtgg ggtcctcgcc gaagacgtcg
    22801 tcaccgacct cggcgtgcgc catggcctgg cgcatggccg gggtcggccg ggtcagggtg
    22861 tccgagagca ggtcggcgcg caccgaggtc agtcctggtt cagcatctcg gcgacgagga
    22921 acgccagctc gaggctctgc tggtggttca ggcgcgggtc gcacaccgtc tcgtagcgca
    22981 ggttcaggtc ggcgtcgagg atcttctcgg acccaccgat gcactcggtg acgtcgttgc
    23041 cggtcagctc gacgtggatg ccgccgggga ccgtgccgag gccacggtgc acctcgaaga
    23101 agccttcgac ctcctcaacg acgtcgtcga agtcgcgggt cttgtagccc gacgccgact
    23161 cgaaggtgtt gccgtgcatc gggtcgcaca cccaggtgat cgaggcaccg gatgccgtga
    23221 ccttctccac gatggtaggc agcgcctcac gaatgcggcc cgagcccatg cgggtgatga
    23281 aggtcaggcg gccgggctca cgctgcgggt cgaccttgtc gatgatccgc agcaggtcgt
    23341 cggtgtcggc gttggccgac accttgatac cgacggggtt gcgcacggag gcgacgaagt
    23401 cgatgtgggc gccgtcgagg tcgcgggtgc gctcccccac ccacacgaag tgtcccgagg
    23461 tgtcgtagag ctcaccggtg cgcgagtcga cccgggtgag cggccgctcg tagtcgagca
    23521 cgagggcctc gtgggcggag aagaactcgg tggtgcgcat cgcctcgaag tcggcaccac
    23581 aggcgttcat gaacttcatc gccttgtcga tgtccttggc caggcgctcg tagcgcgcgt
    23641 tcgcggcgtt ggcgacgaag cccttgttcc agtcgtggac gtggcgcagg tcggcgaagc
    23701 cgccggtggt gaaggcccgc acgaggttca gcgtggcgct gctcgcgtgg taggcccgca
    23761 cgagccgctg ggggtcaggt gtgcgcgact gcggcgtgaa gtcgaagtcg ttgaccatgt
    23821 cgccgcggaa ggcgggcagc gtcacgccgg cgcgggtctc gaagttgctc gaccggggct
    23881 tggcgtactg cccggccatc cggccgacct tgacgacggg caccgacgcg ccataggtga
    23941 gcacggccgc catctgcagg atcgtcttga cccggtcacg gatgttgtcg gccgtggcag
    24001 aggcgaaggt ctcggcgcag tcgccgccct ggagcacgaa ggcctcaccg cgggcgacgg
    24061 ctgccatccg ctcgcgcaag acgtcgcact cgccggcgaa gacgagcggc gggtagctgg
    24121 ccagggtggc cacggcctcg gcgagtgcgt cggcgtcggg ccagaccggc tgctgggcag
    24181 cggggagtcc ggcaccggcg gcgagccgct ggcgggggtc tggtgcggtg gtcgtgctca
    24241 ccgggcaagg ataggtccag tgcggcgccc gcctgcgtct gcccgtatgc cggtcgcgcc
    24301 cgcatccgtt caggcggacg cggcctccgc cctcgccggg cggccagact ccgcgaagac
    24361 ccgctccttt tctgagagag cggacctggg gtctgctgta cgggtcgttg cgtcgggcaa
    24421 tggattcctt cactcatgca acgagcctgc acgcaccccc tcccggggct gatccgcagc
    24481 cttttgcccc cgaggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcc
    24601 ctcagcgaat caggcgcagg gaaccaacgc gtcgacggag gcccacgtgt ggacgcagcg
    24661 ccccgggacc cgtgaccgag ccgtcgccgc gctcagtcct gcgcggggcc ttcatcctcg
    24721 aggccgcggg ccagcgcgaa gcggaccagc tcgacccggt tgtgcaggtg tagcttgccc
    24781 aacgtgttct gcacgtggtt ctgcaccgtg cggtgcgaca ggaacagctc ggccgcgatc
    24841 tccttgtacg acatgcccgt ggcgacgagg cggagcacct cggtctcgcg ctcggtcagc
    24901 tcggggatgg gccggcggcg ctcctcagca gcagccccgg caggcgtggc acccgtgccg
    24961 gacacctcgg atgccgtggc ggtcgccgac gccgacccgt cggccatccg gcggaactcc
    25021 ccgaggacga gcccggcgag ccccggcgtg aacaccgcct ccccgcgggc ggtggcgtgc
    25081 acggcatcga cgatgtcctg tgcggatgcc gacttcacga ggtagccggt ggctcccgcc
    25141 ttgatcgccg caaggacgtc acgctgctca ccgctcgcgg agaggatgag cacccgggtg
    25201 ggcaggtcac cgagggcgtg gcacacctcg tcaccgcgca tccccggcag gttgaggtcg
    25261 aggacgagga cgtcgggccg ggtcgcgcgg gctcggcgca cggtcgacgg gccgtcggtg
    25321 gccgtcgcga cgatgcgcag gccccgctcg gtcaggtccc gttccagggc cgagagccac
    25381 aacgggtggt cgtcggcgac gaggacggtc ggcgcagcgt cacccggggc tgacgcggag
    25441 ggtgaccccc cggcatccgg tggggtcgtg gtcatgctcg ctccttgacg gttcgcggaa
    25501 tggtcatccg gaccacgcac cctccgccgg ggcgggtggt ccacgtgacg gtgccgccga
    25561 ggtcctcgat gcggccgcgg atggatgagc tgaccccgag acgcccccga tcggcggcat
    25621 cgaagacatc ggcgagctgc gcgcccaccc cgtcgtcgcg gatggtgacc tcgaggcccg
    25681 aggagtcacc ctcgagcagc acccacgccc gcgcacccgg cccggcatga cggtcgacgt
    25741 tgtccagggc cgcggcgagc gctgcatcga cctcgtgggc gacgagcacg tcgacgagca
    25801 ccgggtcggc cggcaccgcg acggtgacgt cggggcgctc acgggccgtg agggcgatcc
    25861 gcaggtcggt ctcctccccg tgagccgccc gcaggtcggc gacggcaccg cccgcgtcgg
    25921 gtgccccggt cccggacacg aagcggcgca gcgagcgctc ctgctcggca gcgatcgcgg
    25981 cgaggtcggc ggcctcgccc ccgatgtcct cgccacgccg gtggatgtag gccagtgact
    26041 gcaggacgcc gtcgtggacg acgcgggcga tgcgttcacg ctcgtgcaac cgctcgcgct
    26101 cgagcagcac ctgctcctca ccctcgaggc cacggcgggc gaggtcgacc gccaggccga
    26161 tgaggccgcc gaggagcacg agcaggacga tgttgtggat ggtcgtctgg gtcgggcggc
    26221 ccacctcgat gaggtcgacg atggcgatca ccgctgccgc gatgagccct ccgcgggcgc
    26281 cggcgacgac ggccccaccg gcgaccgagc ccgcggccca gatcgtcggc agggtgaact
    26341 gccccgcggc gatggactcg ggcgtctcgg cgagcaaagt ggccagcacc ccggcgacgg
    26401 cgatcgacac ctcgacgacc acgaccccga tggtgcggcg ccggtagaac agcagcccca
    26461 ccgaccaggc gagcatgacg gcgagcacca ccacggccac ccagggccgc accatcgtct
    26521 cgtggcgctg ccaggccaac cacgcggcat acagcgcagc gagcgggcgg tagacgtcga
    26581 gcccgcggtg gaaggactcc accacgacgg gctcgcgacg tcccgtgcgc tccgagctcg
    26641 gcccgagcac ccccaccacg accccccggt cagctcgcgc ggcgctgcgg cccgctgtcg
    26701 tcgtcgagac cctcggcgtc caggacgccc tccagctcgg agacggcccg gccgggctga
    26761 gccgcctgct gggcctcctt ggccttgctc ttcttctcgc gggcgatgcg gtccttcatc
    26821 gcggtggcgt acatgtcgac gtactcctgg ccggagagct ccatcatcgc gtacatgatc
    26881 tcgtcggtga tcgagcgcag gacgaaccgg tcgccctcca tgccctcgta gcgggagaag
    26941 tccagcggct cgccgatgcg caccccgacg cggatcagct tggggatctt cttgccggtc
    27001 ggctgggcct tgtcggtgtc gatcatcgcc acggggatga cgggcacccc ggcctcgagc
    27061 gccatgcgcg ccaccccggt cttgcccttg tagaggcgac cgtcggggct gcgcgtgccc
    27121 tcggggtaga gcccgagcag ctcgccgcgg cgcagcacct tcagccccga gcgcagcgcg
    27181 gcctcgctgg cgcgcccacc ggagcggtcg acgggaagct gcccgacacc cttgaagaag
    27241 gccgcggtga gccttccctt gatgccttca cccgtgaagt agtcggactt ggcgaggaag
    27301 gtcatccgcc gcggcacgca cagcggcagg aagatcgagt ccgagaagga gaggtggttg
    27361 ctggcgaaga tcgccgcacc ggtctcggga atgttctccg acccctcgac ccacggccgg
    27421 aacagcaggc gaaggatcgg cccgagaatg accgtcttca gcacccagta gaacaaccgg
    27481 accacccttc cttgcgcgtc gcccgcagcc tagtgcactg gatcacatgg ctgtcgtgat
    27541 ctgatcgcat gcgaagatcg tggccccatc ccgcagcacc cgaggagacc ccgtgcccga
    27601 ccggcccggc gacatggacg agcgtgacgt cgacgcgcgc ttcgcctcga tcgtggcgca
    27661 gtgggagcac tcggcggtcg atgagccgga tgccgtggca ccggcaggtg cccccgtcac
    27721 cgacgactcc cccgcggatc ctgcggccgg gcccgagtcc gctgctggct ccgccagctc
    27781 cagcaagtcc gctgtccccg gcgactccgc agaccggacg ccgcgggaag cggacccgtc
    27841 ggagtccgcc gggccggagg cagccgcacc cgcaccacgc gacccctggg tcaacccctc
    27901 ccccctcaac ctggtcgtcc cggcctcggc gtggcgcacc cctgaccccc aggacgccgc
    27961 caccgaacgc gccgccgccg acgccgacga ggccctcgat gcggccatcg ccgcggagga
    28021 gccgcacttc gagccccccg aggtcaccct gcccccgcag gaggacctcg gcttctgggg
    28081 tgccgtcatc ggcctggtcg ccgggccgct catgctgctc tacgtcgtgt tcgcccgccc
    28141 gttccacagc acccggtggt tcgtggcgtc ggt
//
LOCUS       DAOSOC010000013        27885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1713_length_27885_cov_10.520446, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000013 DAOSOC010000000
VERSION     DAOSOC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 27885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27885
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1713_length_27885_cov_10.520446"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PL014_02100"
     CDS             <1..1025
                     /locus_tag="PL014_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOT55916.1"
                     /translation="PFAALLSIPLALMPFAAAGWLWTALQVLATTAIVWYAGYRLIHR
                     ADGWMPLALAALTAPMLWLHPVSDGIRFGQVNAFIVLACLMDLRSPRPGVLRLVPRGV
                     LVGLAMAIKLTPGVFVVHFLVTRRWREAATAVGTAVAVTLGMWVLMPHASFAFWGGAL
                     QDPARLGPNMGTSNQSLRGLLLRIGPEGMPGTVIWLVLVAVVGWLGFALARRLYLQGD
                     SIGEVAAVGLMACLLSPVAWIHHFHWIVVVIFALLGADPLRDRRRLWAGLGVTAFFLC
                     RLPWWGISWLNQPTWPELPGRLLQNADVAGALAALALLWWVTRDEPAEPAEPNGQRTP
                     AISSAE"
     gene            complement(992..1606)
                     /locus_tag="PL014_02105"
     CDS             complement(992..1606)
                     /locus_tag="PL014_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOT55917.1"
                     /translation="MPLDLATVAIVFGTIFVVELPDKTFIATLVMATRFRPLLVWIGV
                     CLAFFVQTAIAVTAGGLLAQLPRTLVEVVAATLFLIGGILLLRGAGDADEEEAETEEE
                     FGAKAAKQVVGWRVVSLCFTILFLAEWGDLSQILTASLVVRYDDPLSVFVGAFLALAT
                     VSGLAAGLGRTLLTRMKLSTIRRVGGTVCLVLAGYSALEIAGVL"
     gene            1680..2633
                     /gene="pheA"
                     /locus_tag="PL014_02110"
     CDS             1680..2633
                     /gene="pheA"
                     /locus_tag="PL014_02110"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOT55918.1"
                     /translation="MTASTSLTPTKRYGFLGPEGTFTSMALDAWGPSAQGERVTFGSV
                     DAALTALRAGSIDAAMVPIENSVEGGVSATLDALASGDPLVVVGEVLVPVTFVLAAAP
                     GTTRDAVRAVGTHSHAWAQVRGWIGENIPDAVYVPTLSTASAAEALEQGPQPFDAAVC
                     APAAARLHGLEVLAEGIGDMQAAVTRFVLVARPGVVPERTGADKTTVVLFQRDDHAGG
                     LLALLEQFAARGINMTRLESRPTKESMGSYCFSIDLEGHVLDERVGEALMGLKRICSE
                     VRFLGSYPRADRQRATIAPLTADEDFSAARAWLTGLRTGRP"
     gene            complement(2712..3731)
                     /locus_tag="PL014_02115"
     CDS             complement(2712..3731)
                     /locus_tag="PL014_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT55919.1"
                     /translation="MGARSIPPPPLAVRRVLHVAWPFVGVLVTGLMVPVIVTGAFMVL
                     VDRRARLFRASCLAVVLMWVDIRMLLGCWALWARTPDQSSPTWREDHEKLFSTALDSL
                     MYYARRWVGLEVVLEDRMHFGSDDEPLLALARHAGPADSLAIAWLLSRTAGRLPRIVL
                     AEALRWDPGIDTILSRLQSFFMPAQARPGQDRAAGLGEMAATMRDNDVFVIFPEGQNW
                     TPTRRAGMIRRLRERGDTALARRAEELQSVLPPRTKGAWAARSARPAADVMVLAHVGL
                     DQLASPRMIWDALPFHDRPFVVRTWTYAADTVPTEAEAFEQWLGDRWDEVDQWVTEHG
                     GTVQA"
     gene            3861..5543
                     /locus_tag="PL014_02120"
     CDS             3861..5543
                     /locus_tag="PL014_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOT55920.1"
                     /translation="MSTPEPCWAAAYGPGVPLHLEAGDTTLVDRFERTASAHPQRVAL
                     EFLGRKTTYAAMADQVSRVAEGLRRLGVGPGDSVALLMPNCPQNVIAFCAVLRLGATV
                     VEHNPLYTAGELRLPFIDHGARVAIVWDKVVPVVEELRGDCALEHVVAVDLTTELPLA
                     KRLALRLPIAKARASRAALTSPAPGALKWAGLTTAPPLAASYPRPGVDDVALLLYTSG
                     TTGAPKGVPLRHRNLTANIDQGRAWVPGLREGEETFLLALPLFHAYGVTVGMLLGIDL
                     AATLVLLPKPEVGLLMNAIERAVPTFVPAVPPLYERIVDEAERRGVSIRGIRYGLSGA
                     MPLPAPLVERWEAATGGLLVEGYGLTETSPVVVGNPMTTDRRPGSIGVPFPNVQVRIA
                     DPEDLDREVPLGERGELLVKGPQVFDGYRGMPEETAASFHDGWFLTGDVVTMDDKGFI
                     TIVDRIKEVIITGGFNVYPSEVEAVLRLHPGVRDAAVVGVMHPESGSEEVVAGIVLDP
                     EAQLDPQAIHDHARESLTAYKVPRRYVVLDELPVNAMGKVLRREVVREIRGR"
     gene            complement(5521..6354)
                     /locus_tag="PL014_02125"
     CDS             complement(5521..6354)
                     /locus_tag="PL014_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT55921.1"
                     /translation="MPRDELTAYVLGGGGVLGSSEVGMVRALAEHGIRPDVVLGTSIG
                     AMNGAFIAADPGVEGAERLAAVWEAVVREGVFLDNPMRQAARVAKYRTHLLSNAPLRH
                     VIERYLPVSRFEELEVAFQCVAACIEDSSGRWFTDGDLTDAVVASCSVPGLFPPVKID
                     DRHYLDGGLVHSIPIGRALALGATRIFVLQVGRVEQPLKVPTKPWEVATVAFEIGRRH
                     RFVHEMESVPDDVALHVLPSGAASSPNVSIGLARIAKMRERMDAAHAASDAYLTALES
                     P"
     gene            complement(6428..9205)
                     /locus_tag="PL014_02130"
     CDS             complement(6428..9205)
                     /locus_tag="PL014_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOT55922.1"
                     /translation="MTTLEPPGTTTAAARPTRQGRTGPPGASRGRWALRDRPGVVWLG
                     LAVLVALVHPFVPAASWLMVHLVLLGALTHSAMVWSTHFAQALLKTPTSLDERARQNA
                     RIGLLVAGQAAVLVGVPTSLWPLTVAGAVAVSSAVGWHGIQLWRRLRHALPGRFRITV
                     RYYLAAAACVPVGATLGAWLARGLDDTTHGRVLVAHSMVMVLGWIGLTVTGTLVTLWP
                     TMLRTRIDERAERLARQALPVLVTAVAVTGSGAALGSRVATLVGLALYAAGLGWWGRA
                     LLAPARQAPPRFFATWSVTAALAWGAVALGLVGWRVATADSWADVAGGYGVVAAVVAA
                     GFGAQLLAGALSHLVPTVLGGGPSVVRAASAELNRGAVWRVTVVNLGLLVCLLPVPSA
                     VRVATSTLVLVALAAFVPLLLRAVKAAVAARRALVATVAEHPVRPERVGPEPRVWSPG
                     QLVAGVATIALALTLGVAIDPAAAGLGPASDAGGPGSLASVGLTGAGSVTPTGETTRV
                     RVEARDMAFSPASITVPAGNRLVIDLVNVDEGSPHDLSFGGDLRTERVMPGRSTTLDV
                     GVVGASGQGWCTIVGHRRMGMVLDVVVQGSAAGASSPGASSADGAVAAEPDDGIRLGQ
                     EPDAAFRAVDAALPPLDGSTVRKVTLTVEEVELEVAPGIRQKRWTFNGGVPGPTLHGR
                     VGDTFVVTLVNKGSMGHSVDFHAGERAPDEVMRTIPPGGSLTYRFTAQRAGIWMYHCS
                     TMPMSAHIAAGMHGAVVIEPDGLPAVDRSYVLVQSEVHVDGDGSTAITEVDADSAAAD
                     TPDAVVFNGVANQYDAAPLTARVGERVRLWVLAAGPNRGSAFHVVGGQFDTVWSEGAY
                     LLRRGGGPLAGEGSTGSEGADGGSQVLGLAPAQGGFVEWSPTEPGSYPFVTHAMADAE
                     RGAHGILAVTR"
     gene            complement(9202..10281)
                     /locus_tag="PL014_02135"
     CDS             complement(9202..10281)
                     /locus_tag="PL014_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55923.1"
                     /translation="MTTPHRAWLLLPAGASLLAGLDAGLLRMGVPAPLDAAALPDVHG
                     PLMVLGFLGTLIALERAVALRAAWGYAAPVLLGLGGVALVAPVPPLLGRMLLLDGTVA
                     LVAVLVALWRRRRDDEVLVQVLAACLAVLAALAWVRVEASVVVPLLAGFVVLTIAAER
                     VELARFHLPRTAGTTLLWLATGLALAATWALVAPGPGSRAFGVALLALVAWLAPRDVA
                     RRTVRSTDLPRFSAVAMLAGYGWLTVAGVAWAVTGVPTTRAAHDLLVHTVFLGFAMSM
                     VLAHAPVILPSVLRRPLPYRRVLWAPLVVLHAGLAVRVCGDLVGQTRLAQAGGVLTTV
                     ALLLLPLTAAASVALPVRHPRSVPA"
     gene            complement(10278..10724)
                     /locus_tag="PL014_02140"
     CDS             complement(10278..10724)
                     /locus_tag="PL014_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOT55924.1"
                     /translation="MCSYCGCESIDVIGRFMAEHVDIINASGVLRRACEHGDNTAVVA
                     AADALAGLLHPHTGAEEAGLFAVLAEDEEFTEHVGSLCAEHTALDAALAAVRGGDHAS
                     FPAFELALRHHIDREENGLFPAAAIAFAGMEWERVTALTPAAPSRP"
     gene            complement(10741..11034)
                     /locus_tag="PL014_02145"
     CDS             complement(10741..11034)
                     /locus_tag="PL014_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HOT55925.1"
                     /translation="MEHTTLPLAGGGQHACGCNESDHGIPELDVRAVPHAIRHATVFG
                     ALSAIPSGGSLDLVAPHDPKPLLAQIADREAGAVTVRYLDEGPDAWRLRLTRA"
     gene            11089..11859
                     /locus_tag="PL014_02150"
     CDS             11089..11859
                     /locus_tag="PL014_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT55926.1"
                     /translation="MGPSPSRVLGDAGEPTPPPLDPARLRVLRELAGADRACTVGELA
                     QALGGHPNTTRHHLRALLAAGLVQVPDAADEAGPIGAGSVREGGRGRPAARYVVTPQG
                     RRTASGVVEGDPAAEEYLALAGAFADRLAALGVDTSEESRAVGRAWGASLAARDVTAA
                     GGGLPGQPREQVVALLHRLGFSPVVRPTPDRDDGREVELRTCPLLDAARRHPEVVCQV
                     HAGLVSGADAAYGGSGEGVRLVPFASPGACHLRLASAR"
     gene            11869..>13091
                     /locus_tag="PL014_02155"
     CDS             11869..>13091
                     /locus_tag="PL014_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HOT55927.1"
                     /translation="MSGAAIFDLDRTLLQGGTGPLLSRAMYDLGVVTRKLPGEKLLYG
                     MFDLIGETLPSIFLARQATLVAKGREMATFDEAAVAAAEAIAEVVHPFALALIEQHQA
                     AGRPVVLATTTPYHLIKPLADRLGFDHVIATRYTINDEGRFDGSITGNFVWSRGKIAA
                     VREWAEREGIDLAESFAYSDSIYDLPLLSAVGSPNAVNPDPRLTLYAVARGWPIVHFD
                     VSPGVFKVPVLGIELQRLLFLASPPLLTPYARFDIAGIENIPRTGPAILVANHRSYFD
                     LQTMIQVLRASGRTARGLGKKEIFDVPGLGAIATALGGIAVDRGTSGQDSFENAAVAL
                     EGGELVALMPQGTIPRGEKFYDPVLKGKTGAARLAAITRAPVIPIGIWGSEQVWPRSA
                     AVPNVLNVTNPPKVRV"
     assembly_gap    13092..13191
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13268..14140
                     /locus_tag="PL014_02160"
     CDS             13268..14140
                     /locus_tag="PL014_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HOT55928.1"
                     /translation="MFVSNALIGLREGLEAALVVVILLAFLKKTDRTWGIPYVWAGVG
                     TAIALSVSLGAVLTFGTRQLTFEAQELVGGIASIIAVAFVTVMVFWMKSAARGLSGEL
                     KGKLDRALDLGPLAVALVGFLGVGREGLETAIFFYATAQAAGAGNNVPLLGWLVGFVG
                     AIALGVLIYRGALRIDLGRFFRYTGIALIFVAGGILSYGIHDLQEAGLLPGLNTLAFD
                     VSGVIDPGAWYAVLLKGTLNFTPATTVLQAIAWVLYVSTVLFFFLRPARPASAPATAP
                     APTVNPVATSRSLP"
     gene            14137..15288
                     /locus_tag="PL014_02165"
     CDS             14137..15288
                     /locus_tag="PL014_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HOT55929.1"
                     /translation="MIARRPLHVVALIAAPAALALGACSAATTTDAASGASGGAITVT
                     ATDTTCEVSRTEAPAGRVEFTVTNTGSKVNEFYVYAEGDRILGEVENIAPGLTRTFHV
                     ELAEPGTYETACKPGMVGDGIRAPFTITGEAAAPRSDDENLTAATTEYQRYVASQADA
                     FLASTSEFVALVKAGKVDEAKALYPTARSSWERIEPVAESFGDLDPKIDGRAEVEAEG
                     MEFTGFHRLERDLWETGLQPDSAAMADRLLTDVTELVTRAKAVELNPLQLANGSKALL
                     DEIATGKITGEEERYSHTDLWDFEANVEGSTAAIAALRPYLATADPALVATIDERGKA
                     LSDLLETYREGDGFVLYTDLTPQDVKKLTTALDAFSEPVATVAGVVARG"
     gene            15285..16559
                     /gene="efeB"
                     /locus_tag="PL014_02170"
     CDS             15285..16559
                     /gene="efeB"
                     /locus_tag="PL014_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HOT55930.1"
                     /translation="MSGSTRRAVIASGAGAAVAGAAAGLAYAARPSAAAASPAAGGGG
                     AGTDAGIVAFRGERQAGITTAAQDRLHLVALDVVTDDPDRLRDLLTRWTRAAERMTYG
                     AEAAPGGVVGGGPWKVPEDTGEALDLAPGHLTVTIGYGPTLFDERFGLADRRPEALRD
                     LPAFRGDDLDPATSGGDIVIQACSDDPQVAVHAVRNLVRLGFGVVAVRWSQLGFGRTS
                     STSRSQATPRNMFGFKDGTANITAEDAAALDEHVWVAPEDVAGTAEWLAGGTYLVARR
                     IRMHIEVWDRTPLMEQEQVFGRDKRVGAPLGGTDEFEEPDFTRLGTDGMPRIPATSHI
                     QLAHHSRLGGVRILRRGYNFTDGTDGQGHLDAGLFFIAFMRDAHRQFVPMQRALARAD
                     ALNEYVEHTGSALFACPPGLGPDDDWGSQLFS"
     gene            16639..18069
                     /gene="zwf"
                     /locus_tag="PL014_02175"
     CDS             16639..18069
                     /gene="zwf"
                     /locus_tag="PL014_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOT55931.1"
                     /translation="MTAPTTVVLFGATGDLAKRKLIPGLLHLFQSGLVDDLRVVGTSL
                     DEMNGDAFRDLALIAIREHSTRDISDADWREFAQRLDYVSISAGPDALREAVARAEAL
                     LPGEVNQRLHYLSVPPKAALAAVRTIAEADLAVRSRVVMEKPFGTDYASAVSLNAQLH
                     EVFDEEQIFRIDHFLGKEAAQNILAFRFANGLFEPIWHRNNISHIEIDVPETLGLTQR
                     GDFYEATGAYRDMVVTHLFQILAFTAMEPPTSLEPSAISREKNKVFRSMQPIQPSDVV
                     RGQYTGYHDEPGVKPDSETETFVALKCFVDNWRWAGVPFYLRTGKRMAEGARIISIAF
                     REPPQSMFPPGSGVGDNGPDHLTFDLADKSRMSLSFYGKRPGPGFRLDKLSMQFSMQE
                     TSWAGAVLEAYERLIYDAARGDRTLFTSASGIERLWEISQPLLDNPPTVRPYSQGTWG
                     PNQIHQLISPYTWRLPFERAWRERKF"
     gene            complement(18116..19249)
                     /locus_tag="PL014_02180"
     CDS             complement(18116..19249)
                     /locus_tag="PL014_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOT55932.1"
                     /translation="MSDAWWTWVGIAVAVVFVGMGAWLVLSSRRDARAAASRPARDSF
                     RREEEAPAPPKKRLAVVLNPTKLDGDGAAEKATIAGVCADQGWAEPLFLPTSEHDVGF
                     GQTRQALEEKVDVVCAFGGDGTVRAVAQEMIGTGVPMGLFAGGTGNLLARNLELPTDD
                     LARAVTVALTGRNRHIDAGWLTLDPHESHLDEHVGEGSSTGPDRHAFLVMAGLGLDAA
                     IMEDTSEQLKARIGWTAYVPAGLKNLLVERFKARVSIDGAPPMTARARTVLVGNCGKL
                     TGGINLMPDAEPDDGTLDVVVIAPRGLAAWVSVAARVLAKSGRSTQTLDRYRCREIEV
                     HVDHDQMVELDGDIIGEARHIQIEVQPRALIVRTESGRSNTAA"
     gene            19306..19878
                     /locus_tag="PL014_02185"
     CDS             19306..19878
                     /locus_tag="PL014_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOT55933.1"
                     /translation="MTSSSPDAAPEPLVDPAITPDDKDWTWTLRERCPDCGFLATDVE
                     PADIARHVTASTAPWEQVLTRSDATARPDPATWSPLEYACHVRDVCRLFEQRARLMLE
                     QDAPTFDNWDQDETAVADRYGEQDPAVVARELIEAAAGLSGVYASVAGEDWERTGIRS
                     NGSLFTVATLGQYGLHDLVHHLWDVGVDLG"
     gene            19875..21185
                     /locus_tag="PL014_02190"
     CDS             19875..21185
                     /locus_tag="PL014_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT55934.1"
                     /translation="MSPELIYLLLGGSLLLAAVLPRVLHRWALSAPMVLVATGMLVGL
                     LPAWDAVVLDPVAARPVIEHVTELTVLVALMGVGLALDRPLRWRSRESWRSWSPAWRL
                     LGIAMPLTIAGVALLAWGPLGVAAPAAVLLGAALAPTDPVLASDVQVEGPTLEGAVDE
                     IDEDDEVRFALTSEAGLNDGLAFPFVYAAIFLASVGSVSQWWAGWLAWELVGKVVVGV
                     LVGVAVGWVLARIAFRAPARSLRLAEVGEPLLALAALLLAYGASEVAGGYGFLAVFAC
                     ALTVRAAERDHDYQRHMHDVIDRLETLLTLVVLLGLGFALTNGLLAHLDWRGVLIGVS
                     LVLVIRPVAGYLAFLGHRTQVGDAGLDHRERLVVAFFGVRGVGSLYYVAYAAGAASFP
                     EERWLWSTVGFTVVLSVVVHGVLATPVMRRLDAARGEPTASALG"
     gene            21271..21912
                     /locus_tag="PL014_02195"
     CDS             21271..21912
                     /locus_tag="PL014_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOT55935.1"
                     /translation="MPSASTLAAFAVASVIIIAIPGPSVLFTIGRALSAGRREALLTV
                     VGNALGILTQICALAIGLGPLIAASTVAYTALKVVGAGYLIWLGVQAIRNRRALASAF
                     EAGVPVTAPSLHAVRAGYVVGVTNPKTIVFFAALLPQFVVPTSAVLPQLVALGAVFAV
                     LALLMDGAVALVASRARDWFASSPKRMERVGGAGGLMMLTLGGGLLLTGRPTT"
     gene            21949..23217
                     /gene="serS"
                     /locus_tag="PL014_02200"
     CDS             21949..23217
                     /gene="serS"
                     /locus_tag="PL014_02200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT55936.1"
                     /translation="MIDIKLLRDNPDAVRASQRSRGEDEGVVDAIRAADERRRSSLTA
                     FEQLRAEQKTMGKDVARAQGEEKQALLARTKEIAAQVKALQADADAAQVELDTLLRTI
                     GNVVEPDVPSGGEDDYVVLETVGTPRDFAAEGFEPRDHLALAEGLDAIDMGRGAKVAG
                     ARFYFLKGAGARLEMGLLGLAMQRAIAAGFVPMITPTLVKSDVMAGAGFLDHHADEVY
                     RLEADDLYLTGTSEVALAGYHADEVLDLSAGPLRYAGQSTCYRREAGSYGKDTRGIIR
                     VHQFNKIEMFVYCRPEDAVAEHQRLLAFEKEMLAAIEVPYRVIDTAAGDLGGPAARKF
                     DCEAWVPTQGRYRELTSTSNCTTFQARRLNTRVKGEAGNEVAATLNGTLATTRWIVAI
                     LENHQQPDGSVIVPEALRPHVGLDVLVPGA"
     gene            23394..24743
                     /locus_tag="PL014_02205"
     CDS             23394..24743
                     /locus_tag="PL014_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT55937.1"
                     /translation="MPPARLPLHSRVGEFFGVDDPWQRPRPRVSHSDVILALATAVLG
                     LVSLELVRSIGVLEAVDPPVWAQILATVSASALLTWRRRWPLSIAVAAAVHMFVVGVT
                     MAPVMGQFTLQVVYFIAFYAAVAWSRDRRLMLGVVGSIIVVMFVWIAWQFLLGSGVQD
                     IVDDTRGVQRIGLVGPVTAAVALTVLINVIYFGGAVIAGQLDWRAARQRAQLETASAT
                     IATQSETLQRRAVTDERLRIARELHDVVGHHISVIGIQAAAARRVLTRDPDAAATALA
                     AIETSSRDGVTQMRSLLGTLRDPEDADPDTATSRMPEPGIADLPTLVAQRRTVGLATS
                     YDVVESSPGAADRLPSSTGLALYRVVQEALANVTRHSTARHATVVLRVEEDGPRPHAE
                     VEVLDDGRPRGRSSGSGLGHLGVRERAASQRGEAEIGPRPTGGYRVRVRIPLGDNSV"
     gene            24745..25545
                     /locus_tag="PL014_02210"
     CDS             24745..25545
                     /locus_tag="PL014_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT55938.1"
                     /translation="MHPPLRVLIVDDQHLVRSGFAMILSVEDDLEVVGEAENGRVALA
                     QARALRPDVVLMDVQMPVMDGIEATRALVAEDLAKVLILTTFDRDDYVFDALSAGASG
                     FLLKNAEPDQLVEAVRAVGHGHALLAPEVTRRVIERMARSGRSGAGPTSTTGAGIAGA
                     PTTQPGAPASGPAASSAPASERAPGLRGAAVDARLDGLSRLTDREREVLVLAARGLSN
                     SEIAAHLVLGEATVKTHVSSCLAKLHVRDRVQAVVVAYEAGLVLPAEA"
     gene            25618..26505
                     /locus_tag="PL014_02215"
     CDS             25618..26505
                     /locus_tag="PL014_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT55939.1"
                     /translation="MAQIETRGLTRTFGDVRAVDGVDLVVRPGGLTGFVGGNGAGKTT
                     TMRMIMGVLRPDAGQVLWGDRPMTAADRQRFGYMPEERGLYPKQKVAEQLAYLGQLSG
                     MSSAAAKSAVAEHLELFSLGDRAGERVEALSLGNQQRVQIIAALMHGPTALILDEPFS
                     GLDPAAVDAMADILRDRAASGVPVLFSSHQLDLVERLCDSLIVLARGRVVAQGAVETL
                     RAGGPTRYRLVTAGDVGWVRDQRGIHVHDVDGGRALLELIDPGAEQAVLRAAVDRGPV
                     LEFARVVPPLSEIYREVTA"
     gene            26502..27704
                     /locus_tag="PL014_02220"
     CDS             26502..27704
                     /locus_tag="PL014_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55940.1"
                     /translation="MSTQTTQPSSATEVGQPWLLVARREVATKLVDRSFLVGTLLTLA
                     LIIGFVGLQAVLAERTKTYDVVVTSSAQTMADAVARQAPTLDDGIRVTVVPAADDAAA
                     EAAVRADDADAWLTERDGSWLLVARDEVPSGLESVTATVVRDAVLTQNADRAGTSVAA
                     LTAGTTLESDVLVGDADQRGFAQGMAFALAVLFYMASLIFGMTLANSVVEEKQSRIVE
                     IIATKIPVRHLLAGKIAGNTVLAVGQMALYAAIGLVGLRFTPYSSFLPSVSGALGWFL
                     VFFLLGFLLISCLWAVAGALASRTEDVQSTSTPVTMLLVAIFFGSLLLDGPAQTVLSY
                     VPPASAILMPQRVLEGSAQWWEPLVALGILAVAAGAVVLLAERLYRRSLLQTQGRLSM
                     RQAWSAPE"
BASE COUNT         3756 a        10044 c        10102 g         3883 t
ORIGIN      
        1 cgccgttcgc ggcgctgctc tcgatcccgt tggcgctcat gccctttgcc gcggccgggt
       61 ggttgtggac cgcgttgcag gtgctcgcga cgacggccat cgtctggtac gccgggtacc
      121 ggctgatcca ccgggcggac ggctggatgc cgctcgcgct cgccgccctc acggcgccga
      181 tgctgtggct gcacccggtg agcgacggca tccgcttcgg ccaggtcaac gcgttcatcg
      241 tgctcgcgtg cctcatggac ctgcgctcgc cccgcccggg ggtgctccgg ctcgtgcccc
      301 gcggggtgct cgtcgggttg gcgatggcga tcaagctgac gcccggggtg ttcgtcgtgc
      361 acttcctggt gacccggcgc tggcgcgagg ccgcgactgc ggtcggcacg gcggtcgcgg
      421 tgacgctcgg gatgtgggtg ctcatgccgc acgcctcctt cgcgttctgg ggtggggcgc
      481 tgcaggaccc cgcccggttg gggccgaaca tgggcacgtc caaccagtcg ctgcgcgggc
      541 tgctgctgcg catcggcccc gaagggatgc cgggcacggt gatctggttg gtgctcgtcg
      601 ccgtcgtggg gtggctgggt ttcgcgctgg cgcgacgcct gtacctgcag ggtgactcga
      661 tcggtgaggt cgccgcggtg gggctgatgg cctgcctgct gtcgccggtg gcctggatcc
      721 accacttcca ctggatcgtc gtcgtcatct tcgccctgct cggggccgac ccgctgcgag
      781 accggcggcg gctgtgggcc gggctcgggg tgacggcgtt cttcctgtgc cggctgccgt
      841 ggtggggcat ctcgtggctg aaccagccga cctggcccga gctgcccggg cggttgctgc
      901 agaacgccga tgtggccggg gcgctcgcgg cgctggcgct gctctggtgg gtgacccgcg
      961 acgagccggc cgaaccagcc gagccgaacg gtcagaggac cccggcgatc tccagcgcgg
     1021 agtagcccgc cagcaccagg cacaccgtgc ccccgactcg gcggatcgtc gacagcttca
     1081 tccgggtgag cagggtgcgc ccgagcccgg cagccagccc cgacacggtg gccagcgcca
     1141 ggaaggcccc gacgaacacc gagagcgggt cgtcgtagcg cacgaccagc gacgccgtga
     1201 ggatctgcga caggtcaccc cactcggcga ggaacaggat ggtgaagcac aggctcacca
     1261 cccgccagcc cacgacctgc ttggcggcct tggcgccgaa ctcctcctcg gtctcggcct
     1321 cctcctcgtc ggcatcgccg gccccgcgca gcagcaggat gccgccgatg aggaacagcg
     1381 tcgcggcgac gacctcgacg agggtgcgcg gcagctgggc gagcaacccg ccggcggtca
     1441 cggcgatcgc ggtctgcacg aagaacgcga ggcagacccc gatccacacg agcagcggcc
     1501 ggaaccgggt ggccatcacc agggtggcga tgaacgtctt gtcggggagt tcgaccacga
     1561 agatcgttcc gaagacgatg gcgacggtcg cgaggtcgag aggcacggca tccaccctag
     1621 ttttgccgcg gggtggaatg cgcgggcgtc cggattcgcc acggcctagc ctgtcgcgtg
     1681 tgaccgcctc gacctcgttg acccccacca agcgctacgg cttcctcggc cccgagggca
     1741 ccttcacgtc gatggccctg gacgcgtggg gcccgtccgc gcagggtgag cgcgtcacgt
     1801 tcgggtcggt ggatgccgcg ctgaccgccc tgcgcgccgg cagcatcgac gccgccatgg
     1861 tgccgatcga gaactccgtc gagggcgggg tctccgccac gctcgacgcc ctggccagcg
     1921 gcgacccgct cgtcgtcgtc ggcgaggtgc tcgtgcccgt caccttcgtg ctggccgccg
     1981 cgccgggcac cacgcgcgac gccgtgcgcg ccgtcggcac ccactcccac gcgtgggcgc
     2041 aggttcgcgg gtggatcggg gagaacatcc cggatgccgt gtacgtgccg acactgtcga
     2101 ccgcatccgc ggcggaggcc ctggagcagg ggccgcaacc gttcgacgcg gccgtgtgcg
     2161 cccccgccgc cgctcggctc cacgggctcg aggtgctcgc cgaggggatc ggcgacatgc
     2221 aggccgccgt cacccggttc gtgctcgtgg cccggcccgg cgtcgtgccc gagcgcaccg
     2281 gggccgacaa gacgaccgtg gtgctcttcc agcgcgacga ccacgccggc gggttgctcg
     2341 cgctgctcga gcagttcgcc gcccgcggga tcaacatgac ccggttggag tcgcgcccga
     2401 cgaaggagtc gatggggtcc tactgcttct ccatcgacct cgaggggcac gtgctcgacg
     2461 agcgggtggg ggaggcgctc atgggcctga agcggatctg ctcggaggtg cgcttcctcg
     2521 ggtcctaccc gcgcgcggac cgccagcggg ccacgatcgc cccgctcacg gccgacgagg
     2581 acttctcggc ggcccgggca tggctgacgg gtttgcgcac tggtcgtccc tgagtcgatt
     2641 cccttgcctg cgtccggcag aacggtggtc agagccacca aactgccgga cgtcacgcgg
     2701 ttgaggcggc gctacgcctg gacggtgccg ccgtgctcgg tgacccactg gtcgacctcg
     2761 tcccaccggt caccgagcca ctgctcgaag gcctcggcct cggtcggcac cgtgtcggcg
     2821 gcgtaggtcc aggtccgcac gacgaacggg cggtcgtgga agggcagggc gtcccagatc
     2881 atccgcgggc tggcgagctg gtcgagcccg acgtgggcca gcaccatgac gtccgccgcc
     2941 ggtcgggccg accgggcggc ccaggcgccc ttggtgcgcg ggggcagcac cgactgcagc
     3001 tcctcggcgc ggcgggccag ggccgtgtcg ccacgctcgc gcagccgacg gatcatcccg
     3061 gcgcgacggg tcggggtcca gttctggccc tcggggaaga tgacgaagac gtcgttgtcc
     3121 cgcatcgtcg cggccatctc acccagaccg gcggcgcggt cctgaccggg gcgggcctgc
     3181 gcgggcatga agaacgactg cagccgcgac aggatcgtgt cgatgcccgg gtcccaccgc
     3241 agcgcctcgg cgagcacgat gcgcggcaac cgcccggcgg tgcgcgacag cagccacgcg
     3301 atggccagcg agtcggccgg gccggcgtgc cgggccaggg ccagcagggg ctcgtcgtcg
     3361 gagccgaagt gcatccggtc ctcgagcacg acctcgagcc cgacccagcg gcgcgcgtag
     3421 tacatcagtg agtccagcgc ggtcgagaac agcttctcgt ggtcctcgcg ccacgtcggt
     3481 gacgactggt cgggggtcct ggcccacagc gcccagcacc ccagcagcat ccggatgtcg
     3541 acccacatga gcacgacggc caggcacgag gcgcgaaaca gccgggcccg ccggtcgacg
     3601 agcaccatga acgcacccgt gacgatgacg ggcaccatca gcccggtgac gagcaccccg
     3661 acgaacggcc acgccacgtg cagcacgcgt cgcacggcca gcgggggagg gggaatgctg
     3721 cgggcgccca tggcggtcag cgtatgccca ccggatgccg tcgacacctc accgccgcag
     3781 ccgcaccgcc gccggtcccg gagtaggttg agggccgtcc gcgggctccc cgacaccgcg
     3841 gacggtgagg acgaacggcc atgagcactc ccgagccctg ctgggctgcg gcctacggac
     3901 ccggggtgcc gctgcacctc gaggccggtg acaccaccct cgtcgaccgg ttcgagcgga
     3961 ccgccagcgc gcacccgcag cgggtggcgc tggagttcct cggccggaag acgacgtatg
     4021 ccgcgatggc cgaccaggtc tcgcgcgtcg cggagggcct gcgtcgtctc ggcgtcgggc
     4081 cgggtgacag cgtcgccctc ctcatgccga actgcccgca gaacgtcatc gccttctgcg
     4141 ccgtgctgcg gctcggggcg accgtcgtcg agcacaaccc gctctacacg gccggtgagc
     4201 tgcggctgcc gttcatcgac cacggcgcga gggtcgcgat cgtctgggac aaggtcgtcc
     4261 ccgtcgtcga ggagctgcgc ggtgactgcg ccctcgagca cgtcgtcgcc gtcgacctca
     4321 cgaccgagct gcccctggcg aagcggctgg ccctccggct cccgatcgcg aaggcccgtg
     4381 cgtcgagggc ggccctgacg agccccgcgc ccggtgcgct gaagtgggca gggctcacca
     4441 ccgcgccccc gctggcggcc tcgtaccccc gcccgggggt ggacgacgtc gcgctgctcc
     4501 tctacacctc gggcacgacg ggtgccccga agggcgtgcc actgcggcac cgcaacctca
     4561 ccgcgaacat cgaccagggc cgcgcctggg tgccggggct gcgcgagggt gaggagacct
     4621 tcctgctcgc cctgccgctc ttccacgcgt acggcgtcac cgtggggatg ctcctcggca
     4681 tcgacctcgc cgcgaccctc gtcctcctgc ccaagcccga ggtggggctg ctcatgaacg
     4741 ccatcgaacg ggccgtgccg acgttcgtgc cggcggtccc gccgctctac gaacgcatcg
     4801 tcgacgaggc ggagcggcgt ggggtgagca tccgcggcat ccgctacggc ctgtcgggcg
     4861 cgatgccgct gccggccccg ctcgtcgagc gctgggaggc ggcgaccggt gggttgctcg
     4921 tcgaggggta cgggctcacc gagacctcac ccgtcgtcgt cggcaacccg atgacgaccg
     4981 accggcggcc ggggtcgatc ggcgtgccgt tcccgaacgt ccaggtgcgc atcgccgacc
     5041 ccgaggacct cgaccgagag gtgccgctcg gcgagcgcgg cgagctgctc gtcaagggcc
     5101 cgcaggtgtt cgacggctac cgcgggatgc cggaggagac ggctgcctcg ttccacgacg
     5161 gctggttcct caccggcgac gtcgtgacga tggacgacaa ggggttcatc acgatcgtcg
     5221 accggatcaa ggaggtcatc atcaccggtg gcttcaacgt ctacccctcc gaggtcgagg
     5281 ccgtcctgcg cctgcacccg ggcgtgcgcg acgccgcggt cgtcggggtg atgcaccccg
     5341 agagcggcag cgaggaggtc gtcgcgggca tcgtgctcga ccccgaagcc cagctggacc
     5401 cccaggcgat ccacgaccac gcccgcgagt ccctgaccgc gtacaaggtg ccgcgacgct
     5461 acgtcgtgct cgacgagctg cccgtcaacg cgatgggcaa ggtgctgcgc cgcgaggtgg
     5521 tcagggagat tcgagggcgg tgaggtaggc atcggatgcc gcgtgcgcgg catccatgcg
     5581 ttccctcatc ttcgcgatgc gcgccaaccc gatcgacacg ttcgggctgc tggcggcgcc
     5641 gctgggaagc acgtgcagcg cgacgtcgtc gggaacggac tccatctcgt ggacgaaccg
     5701 gtggcggcgg ccgatctcga aggcgacggt cgcgacctcc cacggcttgg tcggcacctt
     5761 caggggttgc tcgacccgcc cgacctggag cacgaagatg cgggtcgcgc ccagggcgag
     5821 ggcccgcccg atggggatgg agtgcacgag cccgccgtcg aggtagtgcc ggtcgtcgat
     5881 cttcacgggc gggaagagcc cgggcaccga gcacgacgcg acgacggcat cggtgaggtc
     5941 accgtcggtg aaccagcggc cgctggagtc ctcgatgcag gcggcgacgc actggaaggc
     6001 gacctcgagc tcctcgaacc ggctgaccgg caggtagcgc tcgatgacgt gccgcagcgg
     6061 tgcgttggac agcaggtggg tgcggtactt cgcgacccgg gcggcctggc gcatcgggtt
     6121 gtcgaggaag accccctcgc ggaccacggc ctcccagacg gcagcgagcc gctcggcccc
     6181 ctcgaccccg gggtcggcgg cgatgaacgc cccgttcatg gcgccgatgc tcgtgcccag
     6241 gaccacgtcg gggcggatgc cgtgctcggc cagcgcgcgc accatgccca cctcgctgga
     6301 gccgagcacc ccgccgccgc cgaggacgta cgcggtgagc tcgtcgcgag gcatgccccc
     6361 aacctacggc ttgtgtccgg caggttggtg gtcagggcaa ccaacctgcc ggacgttggc
     6421 tgtcacctca ccgggtcacc gcgaggatgc cgtgcgcgcc ccgctccgcg tccgccatgg
     6481 cgtgcgtgac gaaggggtac gagccgggct cggtggggga ccactccacg aagccgccct
     6541 gggcgggggc caggccgagc acctggctgc caccgtccgc gccctcactg cccgtgctcc
     6601 cctcgccagc gagcggcccg ccgccacggc gcagcaggta cgcgccctcg ctccacaccg
     6661 tgtcgaactg cccgccgacg acatggaagg ccgacccgcg gttcgggccc gcggcgagca
     6721 cccacaggcg cacccgctcc ccgacgcgcg ccgtgagggg tgccgcgtcg tactggttgg
     6781 ccacgccgtt gaagacgacg gcgtcggggg tgtcggcggc tgccgagtcg gcgtcgacct
     6841 ccgtgatcgc cgtggacccg tcgccgtcga cgtgcacctc ggactgcacg aggacgtagc
     6901 tgcggtcgac ggcgggcagc ccgtcgggct cgatgacgac cgctccgtgc atgcccgccg
     6961 cgatgtgggc gctcatcggc atcgtcgagc agtggtacat ccagatgccg gcccgctgcg
     7021 ccgtgaaccg gtaggtgagc gaacccccgg gtggaatggt gcgcatgacc tcgtccggtg
     7081 cccgctcgcc cgcgtggaag tcgaccgagt gccccatcga ccccttgttg accagggtca
     7141 cgacgaaggt gtcacccacc cggccgtgca gggtcggccc cggcacgccc ccgttgaagg
     7201 tccaccgctt ctgccggatg ccgggcgcga cctcgagctc gacctcctcc accgtgagcg
     7261 tgaccttgcg aacggttgag ccgtcgagag gcggtagcgc ggcatcgacg gcgcggaacg
     7321 cggcatccgg ttcctggccg agtcggatgc cgtcgtcggg ctccgcggcg accgcaccat
     7381 cggcgctgct cgccccgggg ctgctcgccc cggcggcgga gccctggacg acgacgtcga
     7441 ggaccatgcc catccggcgg tggccgacga tggtgcacca cccctgaccg ctggctccga
     7501 cgaccccgac gtcgagggtg gtcgagcggc cgggcatcac gcgctcggtg cgcaggtcgc
     7561 cgccgaagga caggtcgtgg ggcgaccctt cgtcgacgtt gacgaggtcg atgacgaggc
     7621 ggttgccggc gggcacggtg atggaggccg gggagaaggc catgtcccgg gcctcgacgc
     7681 gcacccgggt ggtctcaccg gtcggcgtga ccgatccggc cccggtcagg ccgaccgagg
     7741 cgagggaacc cgggccaccg gcatccgacg cggggcccag gccagcagcg gccgggtcga
     7801 tggcgacgcc gagggtcagc gcgagcgcga tggtggccac cccggcgacg agctgccccg
     7861 gtgaccacac ccgcggctcg ggcccgaccc gctcggggcg caccgggtgc tcggcgacgg
     7921 tggcgacgag ggcccggcgg gccgcgacgg ctgccttcac ggcgcgcagc aggaggggga
     7981 cgaacgcggc cagcgccacg aggacgaggg tcgaggtggc gacccggacc gcgctgggga
     8041 cgggtaggag gcagacgagc agaccgaggt tgacgacggt cacccgccag acggcgcccc
     8101 ggttgagctc ggccgacgcc gcccgcacga ccgacggccc gccgccgagc accgtcggca
     8161 cgaggtggct cagcgcgccg gcgagcagct gggcgccgaa gcccgcggcg acgacggccg
     8221 cgacgacgcc gtacccaccg gccacgtcgg cccacgagtc ggcggtggcg acccgccacc
     8281 cgacgagccc gagcgccacc gcaccccagg cgagggcggc ggtcaccgac cacgtcgcga
     8341 agaagcgggg cggggcctga cgtgccggtg cgagcagcgc ccggccccac cagccaagcc
     8401 cggcggcgta gagggcgaga ccgaccaacg ttgcgacgcg cgagcccagc gccgccccgg
     8461 acccggtgac ggcgaccgcc gtgacgagca ccggcagcgc ctgccgcgcg aggcgctcgg
     8521 cgcgctcgtc gatccgggtg cgcagcatcg tcggccacag cgtgacgagg gtgccggtca
     8581 ccgtcagccc gatccagccc agcaccatga ccatcgagtg cgcgacgagc acgcgcccgt
     8641 gggtggtgtc gtccaggccg cgggcgagcc acgccccgag ggtcgcgccg acgggcacgc
     8701 aggcggcggc cgccaggtag tagcgcacgg tgatgcggaa ccgccccggc agcgcgtgcc
     8761 gcagtcgccg ccacagctgg atgccgtgcc agcccaccgc gctcgacacc gccaccgccc
     8821 cggcgacggt cagcggccac agcgaggtcg gcacaccgac gagcaccgcc gcctgaccgg
     8881 cgacgagcag gccgatccgc gcgttctgcc gagcccgttc gtcgagggac gtcggggtct
     8941 tcaggagggc ctgggcgaag tgggtgctcc acaccatggc cgagtgggtg agggcaccga
     9001 ggagcacgag gtgcaccatc agccagctcg cggccggcac gaacgggtgg acgagggcga
     9061 cgaggacggc gaggccgagc cagaccacgc ccggccggtc gcgcagcgcc caccgacctc
     9121 gggaggcgcc gggtggcccg gtgcggccct ggcgggtggg tcgggctgct gcggtcgtgg
     9181 tgccgggggg ctcgagggtg gtcacgcggg gacgctccgg gggtggcgga cggggagtgc
     9241 gaccgaggcc gcggcggtga gggggagcag gagcagggcg acggtggtga gcacgccacc
     9301 ggcctgggcg aggcgggtct ggccgacgag gtcgccgcag acgcggaccg cgagcccggc
     9361 gtgcaggacg acgagggggg cccagagcac ccgtcggtac ggcagcgggc ggcgcaggac
     9421 ggaggggagg atgaccggcg cgtgcgcgag gaccatgctc atggcgaagc cgaggaagac
     9481 cgtgtgcacg aggaggtcgt gggccgcgcg ggtcgtcggc accccggtga cggcccaggc
     9541 gactccggcc acggtgagcc acccgtatcc ggcgagcatc gccaccgcgc tgaagcgggg
     9601 caggtcggtg gagcgcacgg tgcggcgggc gacgtcgcgg ggggcgagcc acgccacgag
     9661 ggcgagcagg gccaccccga aggcgcggct ccccgggccc ggtgcgacga gcgcccacgt
     9721 cgccgcgagg gcgagcccgg tcgcgagcca gagcagggtc gtccctgcgg tgcgggggag
     9781 gtggaaccgg gcgagctcga cccgttcggc ggcgatcgtc agcacgacga accccgcgag
     9841 cagcggcacg acgaccgagg cctcgacccg cacccaggcc agcgccgcga gcacggcgag
     9901 gcacgcggcg agcacctgga cgagcacctc gtcgtcgcga cgccgtcgcc acagcgcgac
     9961 gaggaccgcg acgagggcca ccgtcccgtc gaggagcagc atccggccca ggaggggcgg
    10021 caccggcgcg acgagcgcca ccccgccgag gccgagcagc accggtgcgg cgtaccccca
    10081 ggccgcgcgc agggcgacgg cccgctcgag ggcgatgagc gtgccgagga agccgagcac
    10141 catgagcggc ccgtgcacgt cgggcagcgc cgctgcgtcg agcggcgcgg ggacccccat
    10201 ccgcagcagc ccggcatcga gcccggcgag gaggcttgcc ccggccggca gcagcagcca
    10261 ggcccggtgc ggcgtcgtca tggtcggctg ggggcggccg gggtcagcgc ggtcacccgc
    10321 tcccactcca tgcccgcgaa ggcgatggcc gctgcgggga agaggccgtt ctcctcgcgg
    10381 tcgatgtggt ggcgcagggc cagctcgaag gcggggaagg acgcgtggtc gccaccgcgc
    10441 accgccgcca gcgcggcatc cagtgcggtg tgctcggcgc acagggagcc cacgtgctcg
    10501 gtgaactcct cgtcctcggc gagcaccgcg aagaggccgg cctcctcggc acccgtgtgc
    10561 gggtggagca gcccggccag ggcgtcggca gccgcgacga cggcggtgtt gtcgccgtgc
    10621 tcgcaggccc ggcgcagcac cccggaggcg ttgatgatgt cgacgtgctc ggccatgaag
    10681 cggccgatga cgtcgatgct ctcgcacccg cagtacgagc acacggcgac gacctgccgc
    10741 tcaggcgcgg gtgagccgca ggcgccaggc gtcgggaccc tcgtcgaggt agcgaacggt
    10801 gaccgctccg gcctcgcggt cggcgatctg ggccagcagc ggcttggggt cgtgcggggc
    10861 gacgaggtcg agggagccgc cgctcgggat cgcggagagc gcaccgaaca ccgtggcgtg
    10921 ccggatggcg tgcgggacgg cccgcacgtc gagctcgggg atgccgtggt cgctctcgtt
    10981 gcagccgcac gcgtgctggc cgccgccggc gaggggaagg gtggtgtgtt ccatctggtc
    11041 ctccggtgtc tgattatcta caatgacatt gtgaaaaata ctccgccgat gggtccttct
    11101 ccaagtcggg tgctcgggga tgccggtgag ccgaccccgc cgccgctgga tcccgcgcgc
    11161 ctgcgggtgc tgcgagagct cgccggggcc gatcgcgcgt gcacggtggg cgagctggcg
    11221 caggcgctcg gcggccaccc caacaccacc cgccaccacc tgcgggccct gctcgccgcg
    11281 gggctggtcc aggtccccga cgccgccgac gaggcaggtc cgatcggcgc cgggagcgtt
    11341 cgcgagggtg gtcgcggccg gccggctgcc cggtacgtcg tgacgccgca ggggcggcgc
    11401 acggcatccg gcgtcgtcga gggtgacccc gcggcggagg agtacctcgc gctcgcgggc
    11461 gccttcgccg accggctggc cgcgctcggc gtggacacct cggaggagtc cagggccgtg
    11521 ggccgggcgt ggggtgcgag cctggcggcg cgcgatgtca cggcggctgg tgggggtctc
    11581 ccggggcagc cgcgtgagca ggtggtggcg ctgctgcacc gcctcgggtt ctcacccgtc
    11641 gtgcgcccga cgcccgatcg tgacgacggt cgcgaggtcg agctgcgcac ctgtccgctg
    11701 ctcgatgcgg cgcgacggca tcccgaggtg gtgtgccagg tccatgccgg gctggtgagc
    11761 ggtgcggatg ccgcgtacgg cgggtcgggg gagggcgtgc ggctcgtgcc gttcgccagt
    11821 ccgggtgcat gtcacctcag gctggcgtcg gcccggtaac ctcaccccat gagcggcgcc
    11881 gcgatcttcg accttgaccg cacgttgctc cagggcggca ccggcccact gctgtcgcga
    11941 gcgatgtacg acctcggcgt cgtcacccgc aagctgcccg gcgagaagct gctgtacggc
    12001 atgttcgacc tcatcggtga gacgctgccg tcgatcttcc tggcccggca ggccaccttg
    12061 gtcgccaagg gccgcgagat ggccacgttc gacgaggcgg ccgtggcggc cgccgaggcg
    12121 atcgccgagg tggtgcaccc cttcgcgttg gcgctcatcg agcagcacca ggccgccggg
    12181 cggcccgtcg tgctcgcgac caccacgccg taccacctga tcaagccgtt ggctgatcgg
    12241 ctgggcttcg accacgtcat cgccacgcgt tacaccatca acgacgaggg caggttcgac
    12301 ggctcgatca cgggcaactt cgtgtggtcg cggggcaaga tcgccgcggt gcgcgagtgg
    12361 gccgagcgcg aaggcatcga cctcgccgag agcttcgcct actccgactc gatctacgac
    12421 ctgcccctgc tctcggccgt ggggtcgccc aacgcggtca accctgatcc gcggctgacc
    12481 ctgtatgccg tggcgcgcgg atggccgatc gtgcacttcg acgtctcgcc cggggtgttc
    12541 aaggtgccgg tgctgggcat cgagctgcag cggctgctct tcctggcgag cccgccgctg
    12601 ctcacgccgt acgcccgctt cgacatcgcc ggcatcgaga acatcccccg caccggcccg
    12661 gcgatcctcg tcgccaacca ccggtcctac ttcgacctgc agacgatgat ccaggtgctg
    12721 cgggcctcgg ggcgcactgc ccgcgggttg ggcaagaagg agatcttcga cgtccccggc
    12781 ctcggcgcga tcgccacggc cctgggcggc atcgccgtcg accgcggcac cagcgggcag
    12841 gactcgttcg agaacgccgc ggtcgccctc gagggcggcg agctggtcgc gctcatgccg
    12901 caggggacga tcccgcgtgg cgagaagttc tacgacccgg tgctcaaggg caagacgggc
    12961 gctgcgcggc tggccgcgat cacgcgggca ccggtcatcc cgatcggcat ctggggcagc
    13021 gagcaggtgt ggccccggtc ggcggccgtg ccgaacgtcc tcaacgtcac caacccgccg
    13081 aaggtccgcg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtcgattt
    13201 gacgaggtgc actcactgag gagtacctta cttaggtaag gctcacctct gggaacgggg
    13261 cacgtggatg ttcgtcagca acgccctcat cgggttgcgc gagggcctgg aggcagcgct
    13321 cgtcgtcgtc atcctgctgg ccttcctcaa gaagaccgac cgcacgtggg ggatccccta
    13381 cgtgtgggcc ggggtcggca cggccatcgc cctctccgtg tcgttgggtg cggtgctcac
    13441 cttcggcaca cggcagctga ccttcgaggc ccaggaactc gtcgggggca tcgcgagcat
    13501 catcgccgtc gcgttcgtca ccgtcatggt gttctggatg aagtcggccg cccgcggcct
    13561 ctccggtgag ctcaagggga agctcgacag agccctcgac ctcggccccc tggccgttgc
    13621 cctcgtcggc ttcctcgggg tcggccgcga gggactggag acggcgatct tcttctacgc
    13681 caccgcccag gctgcgggag ccggcaacaa cgtcccgctg ctgggctggc tcgtgggctt
    13741 cgtcggagcc atcgccctcg gggtcctcat ctaccgcggc gccctgcgca tcgacctcgg
    13801 ccgcttcttc aggtacaccg gcatcgcgct catcttcgtc gccggcggca tcctgtcgta
    13861 cggcatccac gacctccagg aggcaggcct cctcccgggc ctgaacaccc tcgccttcga
    13921 cgtctcgggg gtcatcgacc cgggggcgtg gtacgccgtc ctgctcaagg gaacgctgaa
    13981 cttcactccg gcgaccacgg tcctgcaggc catcgcctgg gtgctctacg tcagcacggt
    14041 cctgttcttc ttcctgcgcc ccgcccgccc ggccagcgcg cccgccaccg cacccgcgcc
    14101 aacggtcaac cccgtggcca cctccaggag cctcccgtga tcgcccgccg ccccctgcac
    14161 gtcgtcgccc tgatcgcggc gcccgccgcc cttgccctcg gtgcctgctc cgctgccacg
    14221 acgacggatg ccgccagcgg cgcctcgggc ggtgccatca ccgtcaccgc gaccgacacc
    14281 acctgtgagg tctcccgtac cgaggcaccg gctggccggg tcgagttcac cgtgacgaac
    14341 acgggcagca aggtcaacga gttctacgtc tacgccgagg gcgaccggat cctgggtgag
    14401 gtcgagaaca tcgcccccgg cctgactcgc acgttccacg tcgagctcgc cgagccgggc
    14461 acgtacgaaa ccgcctgcaa gccggggatg gtgggcgacg gcatccgggc gcccttcacc
    14521 atcaccggtg aagcggcggc gcccaggagc gacgacgaga acctcacggc agcgaccacc
    14581 gagtaccagc gctacgtggc gagccaggct gacgccttcc tggccagcac cagcgagttc
    14641 gtcgccctcg tcaaggcagg caaggtcgac gaggccaagg cgctctaccc cacggcccgc
    14701 tcctcctggg agcgcatcga gccggtggcc gagtcgttcg gcgacctcga cccgaagatc
    14761 gacggccgcg ccgaggtcga ggcggagggc atggagttca ccggcttcca ccgcctcgag
    14821 cgcgacctct gggagaccgg gctccagccc gattccgcgg cgatggccga ccgcctgctg
    14881 accgacgtca ccgaactcgt caccagggcc aaggccgtcg agctcaaccc gctgcagctc
    14941 gccaacgggt ccaaggcgct gctcgacgag atcgccaccg gcaagatcac cggcgaggag
    15001 gagcgctaca gccacaccga cctgtgggac ttcgaggcca acgtcgaggg ctccacggcg
    15061 gccatcgcgg cgctgcgtcc ctacctcgcc accgctgacc cggcgctcgt cgcgaccatc
    15121 gacgagcggg gcaaggccct cagcgacctg ctcgagacct accgcgaggg cgacgggttc
    15181 gtgctgtaca ccgacctcac cccgcaggac gtcaagaagc tgaccaccgc cctcgacgcc
    15241 ttctccgagc cggtcgcgac cgtcgctggc gtcgtggcca ggggatgagc ggttccaccc
    15301 gccgggcggt catcgccagc ggcgccgggg cggccgtggc cggtgccgcg gcagggctgg
    15361 cgtatgccgc tcgcccgtcg gccgccgccg cctcaccggc cgccgggggt ggcggggccg
    15421 ggaccgatgc ggggatcgtc gcctttcggg gggagcgaca ggccggcatc acgacggctg
    15481 cgcaggaccg gctgcacctc gtcgcgctcg acgtcgtcac cgacgacccc gaccggctgc
    15541 gtgacctgct gacccgttgg acccgggccg ctgagcgcat gacgtacggg gccgaggccg
    15601 cgccgggcgg cgtggtcggc gggggcccgt ggaaggtgcc cgaagacacc ggcgaggcgc
    15661 tggacctggc gcccgggcac ctgacggtga ccatcggcta cggcccgacg ttgttcgacg
    15721 aacggttcgg cctggccgac cggcgacccg aggcgctgcg cgacctgccg gcgttccgcg
    15781 gtgacgacct cgacccggcg acgtcgggcg gggacatcgt catccaggcc tgctccgacg
    15841 acccccaggt cgcggtgcac gcggtgcgca acctggtgcg gctcggcttc ggtgtggtgg
    15901 cggtgcgctg gagccagctc gggttcgggc gcacgtcctc gacctcccgc agccaggcca
    15961 ccccccggaa catgttcggg ttcaaggacg gcaccgccaa catcacggcc gaggatgccg
    16021 ccgccctcga cgagcacgtc tgggtcgccc ccgaggacgt cgcgggcacg gccgagtggc
    16081 tggctggcgg gacctacctc gtggcgcggc gcatccgcat gcacatcgag gtctgggacc
    16141 gcaccccgct catggaacag gagcaggtct tcggccggga caaacgcgtc ggggcgccgc
    16201 tcggcgggac ggacgagttc gaggagccgg acttcacccg gctgggcacc gacgggatgc
    16261 cgcgcatccc cgcgacgtcg cacatccagc tggcccacca cagtcgcctc ggcggggtgc
    16321 gcatcctgcg ccgcggctac aacttcaccg acggcaccga cgggcagggc cacctcgacg
    16381 ccggcctgtt cttcatcgcc ttcatgcgtg acgcgcaccg gcagttcgtg ccgatgcagc
    16441 gggccctggc ccgggccgac gccctgaacg agtacgtcga gcacacgggg tcggcgctgt
    16501 tcgcgtgccc tccgggtctg gggccggacg acgactgggg atcccagctc ttcagctgac
    16561 cccggatgcc gggtggccgg catccgacga acgacccggg ggtagcggtg cgcggcctcc
    16621 gggcgacagg ctgaccccat gaccgcaccc acgaccgtcg tgctcttcgg tgccacgggg
    16681 gacctcgcca agcgcaagct catcccgggg ctgctgcacc tcttccagtc cgggctcgtc
    16741 gacgacctgc gcgtcgtcgg caccagcctc gacgagatga atggcgatgc cttccgggac
    16801 ctggcgctga tcgccatccg cgagcacagc acccgcgaca tcagtgacgc cgactggcgc
    16861 gagttcgccc agcggctgga ctacgtgtcg atctccgccg ggccggacgc cttgcgcgag
    16921 gccgtggcgc gcgccgaggc gctcctgccg ggcgaggtga accagcgcct gcactacctc
    16981 tcggtgccgc ccaaggccgc cctggcggcc gtgcgcacca tcgccgaggc cgacctcgcg
    17041 gtgcgcagcc gggtggtcat ggagaagccg ttcggcaccg actacgcgtc agcggtctcc
    17101 ctcaacgcgc agctgcacga ggtgttcgac gaggagcaga tcttccgcat cgaccacttc
    17161 ctcggcaagg aggccgcgca gaacatcctc gcgttccgct tcgccaacgg cctgttcgaa
    17221 ccgatctggc accggaacaa catctcccac atcgagatcg acgtgcccga gacccttggc
    17281 ctgacgcagc ggggcgactt ctacgaggcg accggtgcct atcgcgacat ggtcgtgacg
    17341 cacctgttcc agatcctcgc gttcaccgcg atggagccgc cgacctctct ggagccgagc
    17401 gcgatcagcc gggagaagaa caaggtcttc cgctcgatgc agcccatcca gccgagcgac
    17461 gtcgtgcgtg ggcagtacac cggctaccac gacgagcccg gggtgaagcc cgactcggag
    17521 accgagacct tcgtcgcgct gaagtgcttc gtcgacaact ggcgctgggc cggcgtgccg
    17581 ttctacctgc gcaccggcaa gcggatggcc gagggggcgc ggatcatctc catcgccttc
    17641 cgcgagcccc cgcagtcgat gttcccgccg gggtctggcg tcggcgacaa cgggccggac
    17701 cacctgacct tcgacctcgc cgacaagtca cgcatgtcgc tgtccttcta cggcaagcgg
    17761 cccgggccgg gctttcggct cgacaagctc tcgatgcagt tctccatgca ggagacgtcg
    17821 tgggcgggtg cggtgctcga ggcctacgag cggctcatct acgacgcggc gcgcggcgac
    17881 cgaaccctgt tcacctcggc atccggcatc gaacgtctct gggagatctc ccagccgctg
    17941 ctcgacaacc ccccgaccgt gcggccctac tcgcagggca cctgggggcc gaaccagatc
    18001 caccagctca tctcgccgta cacgtggcgc ctgcccttcg agcgggcctg gcgcgaacgc
    18061 aagttctgac cccgatcgct gggagcccgg ggtgccctcc gggtcggcgg tgcggtcagg
    18121 cggcggtgtt gctgcggccg ctctcggtgc gcacgatgag cgcccgcggc tgcacctcga
    18181 tctggatgtg ccgggcctcg ccgatgatgt cgccgtcgag ctcgaccatc tggtcgtggt
    18241 cgacgtgcac ctcgatctcc cggcagcggt agcggtccag ggtctgggtc gagcgcccgc
    18301 tcttggccag cacccgcgcg gcgaccgaca cccacgcggc caggccccga ggggcgatga
    18361 cgacgacgtc gagcgtgccg tcgtcgggct cggcgtcggg catgaggttg atgccaccgg
    18421 tgagcttgcc gcagttgccg acgagcacgg tgcgcgcccg cgcggtcatg ggcggcgcgc
    18481 cgtcgatgct cacgcgcgcc ttgaaccgct cgacgaggag gttcttcagc ccggcgggga
    18541 cgtaggccgt ccagccgatc cgggccttga gctgctccga ggtgtcctcc atgatcgcgg
    18601 cgtccagccc gaggccggcc atgacgagga acgcgtggcg gtccggcccc gtcgacgacc
    18661 cctcaccgac gtgctcgtcg aggtgggact cgtgagggtc cagggtcagc catccggcgt
    18721 cgatgtgccg gttgcgaccg gtcagtgcga cggtcacggc gcgggcgagg tcgtcggtcg
    18781 gcagctcgag gttgcgtgcc agcaggttgc cggtccctcc ggcgaacagc cccatcggca
    18841 cgccggtgcc gatcatctcc tgggcgaccg cgcgcacggt gccgtcgcca ccgaaggcac
    18901 agacgacgtc gaccttctcc tcgagggcct gtcgggtctg tccgaagccc acgtcgtgtt
    18961 cggaggtggg gaggaacaac ggctcggccc acccctggtc agcacacacc ccggcgatgg
    19021 tcgccttctc cgcggccccg tccccgtcga gcttcgtggg gttgagcacc acggccagac
    19081 gcttcttcgg cggggcgggc gcctcctcct cacggcggaa gctgtctcgg gcaggtcggc
    19141 tcgccgcggc ccgcgcgtcg cggcgtgacg acaggacgag ccaggcgccc atcccgacga
    19201 agacgacggc gacggcgatg cccacccagg tccaccacgc gtctgacaca gagtggaagg
    19261 taccgcacga tccgggtgga tcaggtcggc cgtagtctgc ggatcatgac gtcctcctcg
    19321 cccgacgcag cacccgagcc actggtcgac cccgccatca cccccgatga caaggactgg
    19381 acctggacgc tgcgtgagcg gtgccccgac tgcgggttcc tggcgaccga cgtcgagccg
    19441 gccgacatcg cgcgccacgt cacggcatcc accgcgccct gggagcaggt gctgacccgc
    19501 agcgacgcga cggctcggcc cgacccggcc acctggtcgc cgctggagta cgcctgtcac
    19561 gtgcgcgacg tctgccgcct gttcgagcag cgggcccgcc tcatgctcga gcaggacgcg
    19621 ccaaccttcg acaactggga ccaggacgag accgccgtcg ccgaccgcta cggcgagcag
    19681 gaccccgccg tcgtggcccg cgagctgatc gaggccgcgg cagggctgtc gggcgtctac
    19741 gcgagcgtcg cgggggagga ctgggagcgc accggcatcc gctcgaacgg ctcgctgttc
    19801 acggtcgcca cgctcgggca gtacgggctg cacgacctcg tgcaccacct gtgggacgtc
    19861 ggggtcgacc tcgggtgagc cctgagctga tctacctgct gctcggcggc tcgctgctgc
    19921 tcgccgcggt cctcccgcgc gtgctgcaca ggtgggcgct gtcggcgccg atggtgctcg
    19981 tcgcgacggg catgctcgtc ggcctccttc ccgcgtggga tgccgtggtg ctcgacccgg
    20041 tcgcggctcg cccggtcatc gagcacgtca ccgagctcac ggtgctcgtc gcgctgatgg
    20101 gggtggggct ggccctggac cggccgttgc ggtggcgcag ccgcgagtcc tggcggtcgt
    20161 ggtcaccggc ctggcgcctg ctcggcatcg cgatgccgct gacgatcgcg ggggtggcgc
    20221 tgctcgcgtg ggggccgctc ggggtcgcgg cacccgcggc ggtgctgctc ggggcggccc
    20281 tggcgccgac cgacccggtg ctcgcctcgg acgtccaggt cgaggggccg accctcgagg
    20341 gcgccgtcga cgagatcgac gaggacgacg aggtgcgctt cgccctgacg agcgaggccg
    20401 ggctgaacga cgggctggcc ttcccgttcg tgtacgccgc gatcttcctc gcgtcggtcg
    20461 ggtcggtgtc gcagtggtgg gcggggtggc tcgcgtggga gctcgtcggc aaggtcgtcg
    20521 tcggggtgct cgtgggggtg gccgtcgggt gggtgctggc gcggatcgcg ttccgggcac
    20581 cggcccgctc gctgcgcctg gccgaggtcg gtgagccgct gctcgcgctg gcggccctgc
    20641 tcctcgccta cggcgccagc gaggtggccg gaggctacgg cttcctcgcg gtgttcgcct
    20701 gtgcgctcac ggtgcgtgcg gccgagcgcg accacgacta ccagcgccac atgcacgacg
    20761 tcatcgaccg gctcgagacg ctgctgaccc tggtggtgct gctcggcctc gggttcgcgt
    20821 tgaccaacgg cctgctggcc cacctcgact ggcgcggggt gctcatcggg gtgtcgctgg
    20881 tgctcgtcat ccggcccgtc gcgggatacc tggcgttcct cggccaccgc acccaggtcg
    20941 gagatgccgg tcttgaccac cgcgagcggc tcgtcgtcgc gttcttcggg gtccgtgggg
    21001 tcgggtcgct gtactacgtc gcctatgcgg ccggggccgc ctccttcccc gaggagcggt
    21061 ggctctggtc gacggtcggc ttcacggtgg tgctgtcggt cgtggtgcac ggcgtgctcg
    21121 cgacgccggt catgcgacgg ctcgacgccg cgcgaggcga gcccacggca tccgccctgg
    21181 gctgagggct ggggggcgcg aggggtcgcg agggttagcg aggacgaaac aggggtgcgc
    21241 cgggtcgcgc ggttccccca cactgatcgg atgccgtccg cctccaccct cgccgcgttc
    21301 gccgtcgcgt cggtcatcat catcgcgatc cccggcccga gcgtgctgtt caccatcggc
    21361 cgggccctgt cggccgggcg gcgcgaggcg ctgctcacgg tggtgggcaa cgcgctcggc
    21421 atcctgaccc agatctgtgc gctcgccatc ggcctcgggc cactcatcgc cgcgagcacg
    21481 gtggcctaca cggcgctgaa ggtcgtgggt gccggctacc tgatctggct cggggtgcag
    21541 gccattcgca accggcgggc ccttgccagc gcgttcgagg ccggggtgcc ggtcacggcg
    21601 ccgagcctgc acgcggtgcg ggccgggtac gtcgtggggg tcaccaaccc gaagaccatc
    21661 gtgttcttcg cggcgctgct gccgcagttc gtcgtgccga cctcggccgt gctgccccag
    21721 ctcgtggcgc tgggcgcggt gttcgccgtg ctggcgctgc tcatggacgg cgccgtggcc
    21781 ctcgtcgcga gccgggcccg ggactggttc gcgtcatccc ccaagcggat ggagcgggtc
    21841 ggcggcgccg gtgggctgat gatgctcacc ctcggtggcg ggctgctgct caccggccgc
    21901 ccgaccacct gatccgcacg cgtcccggcg cgccgataaa ctcgccgggt gatcgacatc
    21961 aagctgctgc gcgacaaccc cgacgccgtc cgtgccagcc agcgctcccg gggtgaggac
    22021 gagggtgtcg tcgacgccat ccgcgcggct gacgagcgcc ggcggagctc gctgaccgcg
    22081 ttcgagcagt tgcgcgccga gcagaagacg atgggcaagg acgtcgcccg tgcgcagggt
    22141 gaggagaagc aggccctgct cgcccgcacc aaggagatcg cggcgcaggt caaggcgctg
    22201 caggccgatg ccgacgccgc ccaggtcgag ctcgacaccc tcctgcgcac catcggcaac
    22261 gtcgtcgagc cggacgtgcc gagcggcggt gaggacgact acgtcgtgct cgagaccgtc
    22321 ggcacccccc gggacttcgc cgccgagggc ttcgagccgc gtgaccacct cgcgctcgcc
    22381 gagggtctgg acgccatcga catggggcgc ggggccaagg tcgccggggc ccggttctac
    22441 ttcctcaagg gggccggcgc ccgcctcgag atgggcctgc tcgggctggc catgcagcgc
    22501 gccatcgcgg cgggtttcgt gccgatgatc accccgaccc tggtcaagag cgacgtcatg
    22561 gccggcgccg ggttcctcga ccaccacgcc gacgaggtgt accgcctcga ggccgacgac
    22621 ctctacctga ccggcacctc cgaggtcgca ctcgcgggct accacgccga cgaggtgctc
    22681 gacctgtccg cagggcccct gcggtacgcg ggccagtcga cgtgttaccg ccgcgaggcc
    22741 ggctcctacg gcaaggacac ccgcggcatc atccgcgtgc accagttcaa caagatcgag
    22801 atgttcgtct actgccgtcc cgaggatgcc gtggccgagc accagcgcct gctcgccttc
    22861 gagaaggaga tgctcgccgc gatcgaggtg ccctaccgcg tcatcgacac cgcggcgggt
    22921 gacctcggcg ggccggcggc gcgcaagttc gactgtgagg cgtgggtgcc gacccagggc
    22981 cgctaccgcg agctcacgtc gacctccaac tgcacgacct tccaggcccg gcggctgaac
    23041 acccgggtca agggcgaggc cggcaacgag gtcgcggcca cgctcaacgg cacgctcgcg
    23101 accacgcgct ggatcgtggc gatcctcgag aaccaccagc agcccgacgg ttcggtgatc
    23161 gtgcccgagg cgctgcgtcc gcacgtcggc ctcgacgtgc tggttccggg ggcctgactc
    23221 ccgctgaggg cgaccggcag gggtactgct gagagccctt gcgtcggaca agggattcct
    23281 tgcgcggcgc aacgacgtct ccccagaccc cttgggtccg cgcgctcagg aaggtggccg
    23341 ggtcccccct gcgggggagg tgccgggcac tcgggcgtgc ctaggctgcc cgtatgccgc
    23401 ccgcccgtct cccgctgcac tcgcgtgtcg gcgagttctt cggcgtcgac gatccgtggc
    23461 agcgccctcg cccccgggtc agtcacagcg acgtcatcct cgcgctcgcg accgcggtgc
    23521 tcggcctggt ctccctcgaa ctcgtgcgca gcatcggcgt tctcgaggcg gtcgacccac
    23581 cggtgtgggc gcagatcctc gcgaccgtgt ccgcctctgc cctgctcacc tggcggcgtc
    23641 gctggcccct gtcgatcgcc gtggccgcgg cagtgcacat gttcgtcgtc ggcgtgacca
    23701 tggccccggt gatggggcag ttcaccctcc aggtcgtcta cttcatcgcc ttctacgcgg
    23761 cggtggcctg gtcgcgcgac cgacggctca tgctcggtgt ggtcggctcg atcatcgtcg
    23821 tgatgttcgt ctggatcgcg tggcagttcc tcctcggcag cggggttcag gacatcgtcg
    23881 acgacacccg tggcgtccag cgcatcgggc tcgtcggccc ggtgaccgcg gcggtggccc
    23941 tgacggtcct catcaacgtc atctacttcg ggggtgcggt catcgcgggt cagctcgact
    24001 ggcgggcggc ccgacagcgc gcccagctcg agacggcatc cgcgaccatc gccacgcaga
    24061 gcgagaccct gcaacggcgc gccgtgaccg acgaacgcct gcgcatcgcc cgtgagctgc
    24121 acgacgtcgt cgggcaccac atctcggtca tcggcatcca ggcggccgcg gcgcgacggg
    24181 tgctcacgcg cgatccggat gccgctgcca cggctctcgc ggcgatcgag acctcgtcgc
    24241 gtgacggtgt cacgcagatg cgcagcctcc tcgggaccct gcgcgacccc gaggacgccg
    24301 accccgacac cgcgacgagc cggatgccgg agcccgggat cgccgacctg cccaccctcg
    24361 tggcgcagcg gcgaaccgtg ggcctcgcca cctcctacga cgtcgtcgag tccagccccg
    24421 gcgcggccga ccggctgccg tcgtcgaccg ggttggcgct ctaccgggtc gtgcaggagg
    24481 ccctggccaa cgtcacccgc cactccaccg cccgccacgc caccgtggtg ctgcgggtcg
    24541 aggaggacgg cccgcgcccg cacgccgagg tcgaggtgct cgacgacggc cggccgaggg
    24601 gccggtcgtc gggttccggg ctcggtcacc tcggggtgcg tgagcgtgcc gcgagccaac
    24661 gcggtgaagc ggagatcggc ccgcggccga ccggcggcta ccgtgtgcgg gtccggattc
    24721 cactggggga taacagtgtc tgacctgcac ccaccgctgc gcgtgctcat cgtcgacgac
    24781 cagcacctcg tgcgctcggg cttcgcgatg atcctgtccg tcgaggacga cctcgaggtc
    24841 gtcggcgagg ccgagaacgg ccgggtcgcg ctcgcccaag cccgggcgct gcgccccgac
    24901 gtcgtgctca tggacgtgca gatgccggtc atggacggca tcgaggccac ccgggccctc
    24961 gtcgccgagg acctcgccaa ggtgctcatc ctcaccacct tcgaccgcga cgactacgtc
    25021 ttcgacgccc tctcggccgg ggcgagcggc ttcctgctga agaacgccga gcccgaccag
    25081 ctcgtcgagg ccgtgcgggc ggtcggccac ggccacgccc tgctcgcacc cgaggtcacc
    25141 cgccgggtca tcgagcggat ggcccgaagt ggccggtccg gcgcgggacc caccagcacg
    25201 acgggggctg gaatcgccgg tgcgccgacg acgcaacccg gcgcaccggc atccggccca
    25261 gcagcatcca gcgcaccggc atccgagagg gcgcccggcc tgcgcggggc cgcggtggat
    25321 gcgcggctcg acgggctcag ccgcctcacc gaccgcgagc gcgaggtgct cgtgctggcc
    25381 gcgcgagggc tgtccaacag cgagatcgcc gcccacctgg tgctcggcga ggccaccgtg
    25441 aagacccacg tgtcgagctg cctggccaag ctgcacgtgc gcgaccgggt gcaggcggtg
    25501 gtcgtcgcct acgaggcggg cctcgtcctc ccggcggagg cctgacccgc ggggccgccc
    25561 gccgacctca cccggcgggc tgatccaccg ccgcgaccgc ccggctagcg tcgaggcatg
    25621 gcacagatcg agacacgagg gctgacccgc accttcggcg acgtgcgggc cgtggacggg
    25681 gtcgacctcg tcgtgcgccc cggtggcctg accgggttcg tcggcggcaa cggcgccggc
    25741 aagacgacca cgatgcgcat gatcatgggc gtgcttcgac cggatgccgg gcaggtcctg
    25801 tggggggatc ggccgatgac cgccgccgac cgccagcgct tcggctacat gcccgaggag
    25861 cgcgggctct accccaagca gaaggtcgcc gagcagctcg cgtacctcgg gcagctgtcg
    25921 gggatgtcgt cggcggcggc gaagagcgcg gtcgccgagc acctcgagct gttctcgctc
    25981 ggtgaccggg ccggcgaacg ggtcgaggcg ctcagcctgg gcaaccagca gcgggtgcag
    26041 atcatcgcgg ccctcatgca cggccccacc gcgctcatcc tcgacgagcc gttctccggt
    26101 ctcgacccgg ccgccgtcga cgcgatggcg gacatcctgc gtgaccgggc ggcatccggc
    26161 gtgcccgtgt tgttcagcag ccaccagctc gacctcgtgg agcggctgtg cgacagcctc
    26221 atcgtgctcg cccgcggccg ggtcgtcgcc cagggcgccg tggagacgct gcgtgccggt
    26281 ggtcccacgc gctaccgact cgtcacggcg ggtgacgtcg ggtgggtgcg tgaccagcgc
    26341 ggcatccacg tccacgacgt cgacggcggg cgggcgctgc tcgagctgat cgacccgggc
    26401 gccgagcagg cggtgctgcg ggccgccgtc gaccgcggcc cggtgctcga gttcgcgcgg
    26461 gtcgtcccgc ccctgtccga gatctaccga gaggtgacgg catgagcacc cagacgactc
    26521 agccttcatc ggccaccgag gtcggccagc cgtggctgct cgtggcccgc cgtgaggtcg
    26581 cgaccaagct cgtcgaccgc tcgttcctgg tcggcaccct gctgacgctg gcgctgatca
    26641 tcggcttcgt cgggctccag gccgtgctgg ccgagcggac gaagacctat gacgtcgtcg
    26701 tcacctccag cgcccagacg atggcggatg ccgtggcgcg tcaggcgccc acgctcgacg
    26761 acggcatccg ggtcaccgtc gtccccgctg cggacgacgc agccgccgag gcagcggtgc
    26821 gtgcggacga cgcggacgcc tggctcaccg aacgcgacgg gtcgtggctg ctcgtggccc
    26881 gggacgaggt gccgagcggg ctcgagtcgg tgaccgcgac ggtggtgcgc gacgccgtgc
    26941 tcacccagaa cgccgatcgg gcgggcacgt cggtggcggc cctgacggcg ggcacgacgc
    27001 tggagtccga cgtcctcgtc ggcgacgccg accaacgcgg gttcgcccag ggcatggcct
    27061 tcgcgctggc ggtgctgttc tacatggctt cgctgatctt cggcatgacc ctggcgaaca
    27121 gcgtcgtcga ggagaagcag tcacgcatcg tcgagatcat cgcgacgaag atccccgtgc
    27181 ggcacctgct cgcggggaag atcgccggca acacggtgct cgcagtcggc cagatggcgc
    27241 tgtacgcggc gatcgggctc gtcggtctgc ggttcacccc gtactcgtcg ttcctgccct
    27301 cggtcagtgg ggcgctcggg tggttcctcg tgttcttcct gctgggcttc ctgctcatct
    27361 cctgcctgtg ggccgtcgcg ggggcgctcg ccagtcgcac cgaggacgtg cagtcgacct
    27421 ccaccccggt gacgatgctg ctcgtcgcga tcttcttcgg ctccctcctg ctcgacggcc
    27481 cggcccagac ggtgctgtcc tacgtgcccc cggcgtcggc gatcctcatg ccgcagcgcg
    27541 tgctcgaggg gtccgcgcag tggtgggagc cgctcgtggc gctgggcatc ctcgcggtgg
    27601 ctgcgggcgc ggtggtgctg ctcgccgagc ggctgtaccg gcggtcgctg ctgcagaccc
    27661 aggggcggct gtccatgcgg caggcctggt ccgccccgga gtagcggctc gtccccgcga
    27721 acctccttgc gtccggcagg ttggctgctg gagcagccaa cctgccggac gttgccgaca
    27781 cccctccggg gacgtccggc aggttggtgg ccccgaccac cgggttgccg gacgtagcag
    27841 ggtgtgatta aggtgaggct tacctaacca agggttgcct tacct
//
LOCUS       DAOSOC010000014        26782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1803_length_26782_cov_8.644479, whole genome shotgun sequence.
ACCESSION   DAOSOC010000014 DAOSOC010000000
VERSION     DAOSOC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 26782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26782
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1803_length_26782_cov_8.644479"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1341)
                     /gene="tyrS"
                     /locus_tag="PL014_02225"
     CDS             complement(82..1341)
                     /gene="tyrS"
                     /locus_tag="PL014_02225"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT55941.1"
                     /translation="MSDILDELQWRGLVAQSTDESALRTALADGPITAYCGFDPTAPS
                     LHFGNLVQLIVLRHLQRAGHRVICLVGGSTGLIGDPRPSAERVLKTKEQTAQWVANIR
                     RQVEPFLASDGENPAVFVNNLDWTAPLSALDFLRDVGKHFRVNQMVKKEAIAARLNSD
                     EGISYTEFSYQLLQGLDYLQLYRDFGCTLQTGGNDQWGNLTAGSDLIHRVEGASVHLL
                     TTPLLTDSSGEKFGKSAGNAIWLSPEMTSPYAFYQYWVNVEDASVVTLLKVFTDRSRE
                     EIAELERQVEHEPFRRAAQKVLAADVTTLVHGAAATASVQAASEALFGKGDVTALDAA
                     TLRDATAELPGGAVTVGMSVVDALVAVGLVDSRNAARRAIGEGGASVNGVKVTDVERT
                     IASDDVLHDSVVLLRRGRKSLAAGRLG"
     gene            1484..2536
                     /locus_tag="PL014_02230"
     CDS             1484..2536
                     /locus_tag="PL014_02230"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOT55942.1"
                     /translation="MIDNAASTDPLTTFVAGLPKAELHVHHVGSASPRIVADLAARHP
                     DSPVPQDPDELERFFTFTDFAHFIDVYLATVGLLRTDEDVRMLTYEVARDLAAQQVRY
                     AELTMTPYTSVVRGIPIEAYTEAIEDARIAAERDHGVVLRWIYDIPGESGLESADATL
                     TYALDHRPEALVGFGLGGPEIGVPRPQFAPHFAAARSAGLHSVPHSGESTGPQSVWDA
                     LTHLGAERIGHGTTSVQDPRLLEHLAEHRIPLEVCPTSNIATRVVESLDVHPIRAMRD
                     AGVVVTVNSDDPPMFGTTLNREYEIAAHLLDLDENGVADLARDAVDASFAPADVAAAL
                     RAEIASYSEEFSAGRR"
     gene            complement(2517..3158)
                     /locus_tag="PL014_02235"
     CDS             complement(2517..3158)
                     /locus_tag="PL014_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55943.1"
                     /translation="MRPTRMRMVFAAVLAAAALPLAACGSSSSEPSPGAASSQAVPMV
                     TAVPAATAPVTGLGTVIEKPGSPPELCLGPVAESWPPQCQGIPLVGWDWASNPPDSET
                     EAGAPVTRWGTYAVTGTFDGTTLTVTSAVSLALYDTMAQPSPTPVPPPDLSDAQWDAV
                     ESALRATPGLLTSGRSDDRGPVLVSVVHDDGSIQAWADASFGPGAVVVTSALR"
     gene            complement(3158..3784)
                     /locus_tag="PL014_02240"
     CDS             complement(3158..3784)
                     /locus_tag="PL014_02240"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOT55944.1"
                     /translation="MTSTRLPVQFFTRDVLTVARDLLGCRVTRGGVTVRLTEVEAYAG
                     GADPGSHAFRGRSPRTEVMFGPAGRLYVYFTYGMHHCANIVTGQPGTASAVLMRAGEV
                     VAGHELAAERRPGVRERDQARGPANLAMTLGLTRAHDGLSLVGARAGGRVEPPETPVD
                     AATVRTGPRVGVSGAGGDGTAYPWRFWLDGEPTVSAYRVARTRNGRAG"
     gene            complement(3823..5229)
                     /gene="argH"
                     /locus_tag="PL014_02245"
     CDS             complement(3823..5229)
                     /gene="argH"
                     /locus_tag="PL014_02245"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT55945.1"
                     /translation="MWGGRFAGGPADALAALSKSTHFDWRLALHDLAGSRAHARVLHG
                     AGLLDDATLEAMLDALDRLRVDVESGAFVPEHGDEDVHTALERGLIERAGADVGGRLR
                     AGRSRNDQVATLFRMYLREHARVVAGLALDVVDALVAQSTRHLGVAMPGRTHLQHAQP
                     VLLSHHLLAHAWALLRDVDRLRDWDVRTAVSPYGSGALAGSSLGLDPEAVAADLGFDS
                     SVENSIDGTASRDFVAEFAFVAAMTAVDISRLAEEVVLWATKEFSFITLDDAYSTGSS
                     IMPQKKNPDVAELARGKAGRLVGDLTGLMTTLKSLPLAYNRDLQEDKEPVFDAVDTLE
                     VLLPAFSGMVDTMVFDTARLESLAPQGFSLATDIAEWLVREGVPFRVAHEVAGACVRA
                     CEERGIELWDLTDDDLTAISPHLTPGVRDVLSVEGSMASRSARGGTAPVRVAEQLERV
                     VATATDHRRWASAQPTVR"
     gene            complement(5316..5819)
                     /locus_tag="PL014_02250"
     CDS             complement(5316..5819)
                     /locus_tag="PL014_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851441.1"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOT55946.1"
                     /translation="MTISTSRAARQQRIVEILAATPVRSQTELLDHLAADGIEVTQAT
                     LSRDLVDVGAERVRVGKSLVYAVPGEGGDRTVRPATDGEERGTRLQSRCHELLVSAEA
                     SANLVILRTPPGAANFLASALDHATIDGVLGTIAGDDTIMVVTRGASASRDLVDRLMA
                     YTRRDPQ"
     gene            complement(5816..6769)
                     /gene="argF"
                     /locus_tag="PL014_02255"
     CDS             complement(5816..6769)
                     /gene="argF"
                     /locus_tag="PL014_02255"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOT55947.1"
                     /translation="MTLRHLLADDDLSAAEQHEVLQRALALKAEPFSDQCLAGPHTVS
                     ILFDKPTLRTQVSFVGAVAHLGGYPMVIDGRLAGVGERESVADVARILGPQSAAIVWR
                     TFEQSRLEEMAAQAGVPVVNALTDDHHPCQILADLLTITEVKGTTAGLTFTYLGDGAN
                     NMAHSYLLGCALAGMHVRIATPASHQPRADIVARAQRHAAAQGGSILITDDPVVAASG
                     ADVVATDTWVSMGMEAESEQRHGAASPFEPFRVSEALMAKAAPDAVFLHCLPAYRGYE
                     VDASVIDGPSSLVWQEAENRLHAQKALLSWLMEQHRAGDGR"
     gene            complement(6766..8007)
                     /locus_tag="PL014_02260"
     CDS             complement(6766..8007)
                     /locus_tag="PL014_02260"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HOT55948.1"
                     /translation="MSDASTASATTASGEATAAWIERYEGSLLNLFGRPQLVLDHGDG
                     AWVWDVDGRRYLDLVGGIAVNALGHNHPALVAAVSKQAGQLVHVSNFFTTVAQVELAE
                     KLLHLAKAPEGSAVFFCNSGTEALEAAIKLARRTGRTDIVAAEGAFHGRTTGALALTH
                     KPAYREPFEPLIPGVTHVPWGDEAALRAAVGPQTSALVLEPIQGEAGVRPADGAYLRL
                     ARELTHEVGALLILDEVQTGIGRTGTWFGFQHTGVVPDAITLAKGLGGGVPIGALITF
                     GPRVSTMLTPGNHGSTFGGNPLACAAGLAVLDAIESQGLIEHARLAGEHLERRVLALG
                     HPLVTGVRGAGLMRAITLAADVSATVAALAREAGFIVNPVAPDALRLVPPLVITTHEL
                     DLFIDALPELLDAASTLKDTP"
     gene            complement(8000..8941)
                     /gene="argB"
                     /locus_tag="PL014_02265"
     CDS             complement(8000..8941)
                     /gene="argB"
                     /locus_tag="PL014_02265"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOT55949.1"
                     /translation="MPPPDQALRGAIDAAALRVAQSKASTLVEALPWLERFRGALVVV
                     KYGGNAMIDDALKAAFAQDIAFLRFAGLRPVVVHGGGPQIKAMLDRLGLASEFKGGLR
                     VTTPEVMDVVRMVLTGQVGRELVGLLNQHGPVAVGLSGEDAGLFGARRRGVVVDGEEH
                     DIGLVGDVVQVDPTAVLDLLDAGRIPVVSTIAPDLDTDGQVLNVNADTAAAALAAALG
                     ARKLVVLTDVEGVYANWPDRASLLSELSVTRARRLLETVDAGMIPKLEACIRAVEAGV
                     PQAHVVDGRQPHSLLLEVFTSEGIGTMVLPDREVHDE"
     gene            complement(8971..10134)
                     /gene="argJ"
                     /locus_tag="PL014_02270"
     CDS             complement(8971..10134)
                     /gene="argJ"
                     /locus_tag="PL014_02270"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOT55950.1"
                     /translation="MSVTTPLGFRASGVTAGLKASGKPDVALVVNDGPHHHAAAVFTT
                     NRVEAAPVTWSRTVISDGRADAVVLNSGGANACTGAPGFQDTHRTAEHVGEVLGVAAG
                     DVVVCSTGLIGERLPMGLLLPGVEAAVEELSVDGGPAAATAIMTTDSVPKTATAVRDG
                     WSVGGMAKGAGMLAPALATMLVVITTDAVIEGAHVADDLDAVLRSATAMTFDRIDSDG
                     CMSTNDTVLLMASGASGTTVALADLTDAVTQVCADLARQLIADAEGAHHDIAIEVTGA
                     ASEDDALEVARSVARNNLFKCAVFGNDPNWGRVLAAVGTTQALFDPAELDVSMNGLMV
                     CRAGGVGEDRSLVDLTAREVRVVVDLHAGSATATVWTNDLTHDYVHENSAYST"
     gene            complement(10134..10538)
                     /locus_tag="PL014_02275"
     CDS             complement(10134..10538)
                     /locus_tag="PL014_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT55951.1"
                     /translation="MPRHLMEHPEDVSIVRLDGGQLPEFDWAAGPFASLTRTPDETSV
                     VCLTATVPAGVRTEGPFRVVEVAGPLSFGSVGVVAEVVEPLVDADISVLAFSTFDTDW
                     VLVPAGQTDEAAHAWRRAGLIITPTSLTGGSS"
     gene            complement(10544..11584)
                     /gene="argC"
                     /locus_tag="PL014_02280"
     CDS             complement(10544..11584)
                     /gene="argC"
                     /locus_tag="PL014_02280"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOT55952.1"
                     /translation="MKRVAVAGASGYAGGEVVRLLLQHPELEVGALTASSNAGQTLGS
                     IHPHLTPLADRVLEDTTPDVLAGNDAVVLALPHGHSAALAAQLPDDVIIIDCGADFRL
                     ADAAAWDTFYDTPYAGSWPYGLPELATADGPQRLALAGATRIAVPGCYPTAVSLALAP
                     GLVAGVLEADDITVVAASGTSGAGKALKPHLLGAEVMGAMSPYGVGGTHRHTPEIEQN
                     LTAAGAGPVTVSFTPTLAPMPRGILATCTARVRPGTTADAVRAAWATAYADEPFVHLL
                     PPGRWPSTGSVLGSNAVHLQVTLDERVGRVVVVAAIDNLTKGTAGAAVQCLNLALGLP
                     EATGLPVAGVAP"
     gene            complement(11641..12450)
                     /locus_tag="PL014_02285"
     CDS             complement(11641..12450)
                     /locus_tag="PL014_02285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT55953.1"
                     /translation="MSTPVEVPVDDDGEVTPEPVTDDGRGGHLVAVVTGASRGIGRHV
                     ADALEDAGYSVERGSTATAPVSDRAAVSAWVAEILERQGRIDVLVNNAGVIDTEVDVL
                     ASDPDEWWQVQEVNVLGAYLMTRLVGEAMLHQGGGRIININSGAALRPGLDASAYNVS
                     KTALARITGSTHLAGWARGIRAFDLMPGVVRTDMTQAMHAHVGRTEWTAPEDVTDLVL
                     ALASGELDAWSGRFVRAGVDTVESLRERADTLGERDRTLGLVPYTPDDPLA"
     gene            complement(12447..14999)
                     /gene="pheT"
                     /locus_tag="PL014_02290"
     CDS             complement(12447..14999)
                     /gene="pheT"
                     /locus_tag="PL014_02290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT55954.1"
                     /translation="MRVPIDWLREYAAVPHDATGEQIAADLVRVGLEEEGLHTGGVTG
                     PLVVGRVLTKTPEPQKNGKTINWCTVDVGDANGTGEPQGVVCGAHNFDAGDLVVVILP
                     GGVLTTPMGPLTVSARKTYGHVSAGMICSERELGIGDDHDGILVLTKRFAGDEATLAT
                     LTPGTDALPLLGLDRETVEVNVTPDRGYCFSVRGIAREYGHATRAAFTDPVTALDAAA
                     PAATEGGFPVVLDDQAPIRGRAGCDRYVARIVRNIDVSAPTPRWMATRLTEAGMRPIS
                     LPVDVTNYVMLGLGQPLHAFDLDTLQGPIVVRRARAGERLTTLDDAERSLGEGDLLIT
                     DHDGERAIAIAGVMGGAETEVGATTRNVLIEAAHFDAVSIARTARAHKLPSEASRRFE
                     RGVDPALAAAAAQLAVDLLVEHGGGVADPEVTDVGSPAAPDPVTMHVDFPSKIVGIVY
                     LESEVVSILSSIGCSVTREGHLLTVIPPTWRPDLTTAEDLVEEVARIAGYDGIPSIVP
                     TPPGGAGLTHAQRTRRLVADVLAGQGLSEVWSAPFVGADRFADLGLDVEAEVARTVRL
                     ANPLSEEAPLMRTRLLATLVDALRRNVARGSRDVGLFELGLVVALDGPQAAAPTEDVG
                     IHPSAETLAAIRDAVPPQPRHVAFLLAGERERAGWWGPGRPADVTDVVDLARTLGEAL
                     GVAIEVVADTAAPFHPGRCARITLSDGTLVGHVGELHPKAVAALGLPARAVGGELDLD
                     VLTTASEGTPQARTVHTFPLAQSDVAVVIDEDVPAARVAQALRGGAGETLESLTLFDV
                     YRGDQVGEGRKSLAYRLTFRAPDRTLTTDEVSGLRDRALAAAAQAVGAVQRA"
     gene            complement(14999..16093)
                     /gene="pheS"
                     /locus_tag="PL014_02295"
     CDS             complement(14999..16093)
                     /gene="pheS"
                     /locus_tag="PL014_02295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOT55955.1"
                     /translation="MSGPNTSYDPVEVAALDPHAIEEAVTEALAAAAAATSLDELKAA
                     RTAHQGDKSPLALANREIGALPPSAKAEAGKRVGQARGRVAQAFTARQGELEIERDER
                     ILTDEAVDVTLPSGRRPLGRRHPIELMSQRIADLFVGMGWEIAEGPEIEAEWFNFDAL
                     NFDADHPARQMQDTFYVDPPEGGLVLRTHTSPVQARTLLERGVPVYVAAIGRVFRTDE
                     LDATHMPAFHQVEGLAVDEGITMAHLKGTLDRLATELFGEGITTRLRPSYFPFTEPSA
                     EMDLRCFVCRGEDPACRTCKGTGWIEWGGCGMVNRNVLTACGVDPDRYTGFAFGMGID
                     RALMFRTGVSDMRDMIEGDVRFDAQFGMEI"
     gene            complement(16142..17329)
                     /locus_tag="PL014_02300"
     CDS             complement(16142..17329)
                     /locus_tag="PL014_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT55956.1"
                     /translation="MPAVSFAPYRRALRSKPLRTALVLGTLVRMPVFASTVLLTIHVV
                     SSLDRSYTAAGVLAAAATVAIAVSGPWRGRLLDRYGLRRVVLPSILVALVCWSIAPFV
                     DYLLLLGLAVLAGLFVIPTFSITRQAVIAAVGEEERRTAISLDAVAVELSFIVAPAVA
                     VWAATVWDTSWVLFTIQMLGVGAGILMYVVDPPLRGEGEAVVGTARASSRTWFRLPFL
                     AVCLAATATTVVLAGSDISIIAVMRDIAAEGQIGLVLAVWGLGSLIGGLLYGALHRPI
                     SAFWLLAGLAAATFPMALASSTVQLAASGFVAGLLCAPTITATIDQASRIVPAQVRGE
                     AMGWHGSFMTTGGAVGAPLAGLAIDRGGPGAGFVVVAAVGVVVAALGLTAVSVRRATA
                     SAT"
     assembly_gap    17431..17530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17531..18920
                     /locus_tag="PL014_02305"
     CDS             <17531..18920
                     /locus_tag="PL014_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55957.1"
                     /translation="HEMSRDFWSPVEPLVGEDVPFPYFSPWTAVGALGVRLFSVTPFQ
                     VLAVFAVVSPLVLVHSLHRLVRAVSPAPWAPVAVLAALLTVWGTRAFFWSGFLSLNTL
                     VVGSSWPSVLATAFWFYLWAAVWTSTRMTRGLIATLLVVPGVLLLFHPFTFLSAATAC
                     AITVLLRWPRQRWLSGAVPSLISVGLAALWPWISLGDLLDNPRGFDGFHVFFYDDVMA
                     KFGLLLLTIPAIVLRLGRQRTDPLGWSVLAGVGIWAAGGVLHVESLGRVIPLASTAAA
                     VALGVAAAEALQPADAGSTGTARPRGASAARIVVVLAWVVAVAVGSMTQSNAWSRITV
                     DDAARRNPDDGTHLISPYPVVTDLWASIPSGSVVVARDPRVARQLPASGLFSVAPQWP
                     SPGVTDTPRRVDDEAAILSAKTSPELREELLTRYDVGWIVWPDAKKTPTWLAELGVPL
                     DSVAGQTLYDVR"
     gene            complement(18949..20037)
                     /locus_tag="PL014_02310"
     CDS             complement(18949..20037)
                     /locus_tag="PL014_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT55958.1"
                     /translation="MAHPFRVPDDLVVNGLEFLPDGLVAAVGPERTVRFMNSCAERIT
                     GVRVEDAIGGDVREVLPLQDHAGASWWELTDPWNGLASRKGHRERLLVLPSGREVLVT
                     AKYVRPAPGEPVEAVVLGLRDAESRRRAENETAALLTTVAHELRSPLTGVKGFSATLL
                     RNWDRLTDDQKRLMLETIEADADRVTRLITELLDVSRINTRHLTVHPQPLDVAAVLRA
                     HAQRRIASGADAPADADAERFVVDVPDDLGPVWADPDRFEQIVANLVENAVIHGRGTV
                     RLAARAGELDGAPAVDVLVADDGDGIDPQHRELAFSRFWQDGAKQGTGLGLYLVRGLV
                     EAHGGVIVAEQAPGGGAQLRATFPDRDI"
     gene            complement(20048..20899)
                     /locus_tag="PL014_02315"
     CDS             complement(20048..20899)
                     /locus_tag="PL014_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT55959.1"
                     /translation="MLTNPRAERVRSVRALSKRSVRERTGRFAVEGPQGVREAVRYAA
                     ARVRDVYVTSEALERYALDIVEPARAANLWVHEVSAEVLAAMAETDAPQGVLAVVDVV
                     DSDEGADAVGPADALAAVLDAHPRLLVLLTNVRDPGNAGTVIRGADAAGADAVLVSSA
                     SVDIHSSKVVRSTAGSLFHLPVVTGLDVEATLETLRAHGIRTLAADGAGATLLPDADL
                     TAPHCWVMGNEAWGLPDEVRDACDEVVRVPIHGRAESLNLAMAATVCLYASAGQRPRR
                     EGVTTAG"
     gene            complement(20911..21294)
                     /gene="rplT"
                     /locus_tag="PL014_02320"
     CDS             complement(20911..21294)
                     /gene="rplT"
                     /locus_tag="PL014_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT55960.1"
                     /translation="MARVKRAVNAQKKRRVVLERASGYRGQRSRLYRKAKEQVQHSLG
                     YSYRDRRARKGDFRRLWIQRINAAARANGMTYNRFIQGLKAAEVEVDRRMLAELAVND
                     AAAFTALVEISKANQPAREPAKADA"
     gene            complement(21340..21534)
                     /gene="rpmI"
                     /locus_tag="PL014_02325"
     CDS             complement(21340..21534)
                     /gene="rpmI"
                     /locus_tag="PL014_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOT55961.1"
                     /translation="MPKNKTHSGAKKRFRVTGSGKVMREQAGHVHKFHEKTPQKARAL
                     SKDVLVSKGDLKKVKKLLGR"
     gene            complement(21612..22463)
                     /gene="infC"
                     /locus_tag="PL014_02330"
     CDS             complement(21612..22463)
                     /gene="infC"
                     /locus_tag="PL014_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT55962.1"
                     /translation="MSEPRINDRIRVPEVRLVGPNGEQVGIVRVEDALRLAAEADLDL
                     VEVAPMAKPPVAKLMDFGKFKYEAAMKAREARKNQVNTVIKEIKLRPKIDPHDYGTKK
                     GHVERFLKGGDKVKVTIMFRGREQSRPELGYRLLQRLAQDIAELGIVESSPKQDGRNM
                     VMVIAPTKKKAQAMAEQRRRRDEGSAAASAPEQSIEAEVREAEAFVAPAAVVEAAPVV
                     EAPVAEAPAAEDAPAPALAAEVEAPAAAPAPTPAAAKPTAAPKPQAAAPKPKAAPKPR
                     ATPKPKA"
     gene            complement(22764..23534)
                     /locus_tag="PL014_02335"
     CDS             complement(22764..23534)
                     /locus_tag="PL014_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HOT55963.1"
                     /translation="MTNPREHEHHPLHAPAGEATDSADLPWAARELSPSGFDTDTGAA
                     DPALLAALAAPGDDVALMAAVEAGRFLVPIVAEPTEVDTSGEHAVDSQVDLAAVTLVA
                     PDGMRALPVFSGIEAVAAWDPEARPVPVTPARAGQAAVSEGCEVIVVDVAGPATRVLR
                     PSMVWALAQQRRWVPAHTDPFVAASVTAALRDEPDVTASEIEEGNPRGEGVLGVVLDL
                     RPGLGPDAVRAVATRVGEKLATDGELRARIDGLAFRIR"
     gene            complement(23531..24268)
                     /gene="priA"
                     /locus_tag="PL014_02340"
     CDS             complement(23531..24268)
                     /gene="priA"
                     /locus_tag="PL014_02340"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HOT55964.1"
                     /translation="MTSQPILQLLPAVDVVGGRAVQLVQGVAGTGGDFGDPLQAALAW
                     QEQGAQWLHLVDLDAAFGRGSNADLLASIVGTLDIDVEMSGGIRDAESLERALATGCR
                     RVNVGTAALENPEWTARAIAEHGDRVAVGLDVRGTTLAARGWTQEGGDLWETLARLDA
                     EGCARYVITDVAKDGMLQGPNLDLLRDVCARTDRPVIASGGVSTIEDIVAIRGLVGDG
                     VEGAIIGSALYRGAFTLPQALDVAGRA"
     gene            complement(24354..24995)
                     /gene="hisH"
                     /locus_tag="PL014_02345"
     CDS             complement(24354..24995)
                     /gene="hisH"
                     /locus_tag="PL014_02345"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOT55965.1"
                     /translation="MSARVVVLDYGSGNIRSAVRALEHVGAEVSLTGDRDAALAADGL
                     FVPGVGNFHACMAGLIAADGPRIIDQRLSGGRPVLGVCVGMQVMFEGSSEPSATPLAG
                     VGEWPGTVERLRAPVVPHMGWSAVEVAPGSVLFEGIADERFYFVHSYAAHTWALEASD
                     GPFAAVAPRVTWATHGERFIAAVENGPLTATQFHPEKSGEAGLALLRNWVRSL"
     assembly_gap    25127..25226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25227..25773)
                     /locus_tag="PL014_02350"
     CDS             complement(<25227..25773)
                     /locus_tag="PL014_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55966.1"
                     /translation="MAGVARTGEGHGLVLVRQPPAAVNRWLRRGLVAVTVAPFGAWTG
                     ITLAEERARSVAPYDVGLELLAARPAPRRARPALGFFDVHGRAAITVQPPGLRSHIHW
                     LVWEPGAGLVPTPSLTPLRLPLLLSVAGASGRVREEVVAEVLRSGTGAPVDLLVTVLG
                     LLGLPGRDLLVRGDSDGAHDVE"
     gene            complement(25777..26391)
                     /gene="hisB"
                     /locus_tag="PL014_02355"
     CDS             complement(25777..26391)
                     /gene="hisB"
                     /locus_tag="PL014_02355"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOT55967.1"
                     /translation="MSSAPAPRTATVRRGTSESTVEVTVDLDGSGRGQVDTGVPFYDH
                     MLLSLAKHSLIDLSVTATGDTHIDAHHTVEDVAIVLGQAVREALGDKSGIARFGDATV
                     PLDEALVLAVVDVAGRPYCVHEGEPEGQQYVVIGGHYVGSLTRHVLESFAFHAQIALH
                     VRVLGGRDPHHVVEAQFKALARALRAAVARDPRVEGIPSAKGAL"
     gene            complement(26388..>26782)
                     /locus_tag="PL014_02360"
     CDS             complement(26388..>26782)
                     /locus_tag="PL014_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT55968.1"
                     /translation="RLVRMPYHLATQTQAVALAALEHADLMLETVGAIKAERDRLVAE
                     LTAMGLDPVPSDANFVLFGGLEDSHTTWSALLERGVLVRDVGIAHYLRVTAGTPQETT
                     AFLQAMSDLAPQHRTTRTPMTLSPGARP"
BASE COUNT         3735 a         9369 c         9723 g         3755 t
ORIGIN      
        1 aactgtagac cgactccacg gagcccacca aatcggctct cacccgggcg tgtcgccgct
       61 gccgcgagcg ttgcccgcgg gtcagccgag acgccccgcc gcaagggact ttcgaccacg
      121 gcggagcagg acgacgctgt cgtggagcac gtcatccgac gcgatggtgc gctcgacatc
      181 ggtgaccttc accccgttca ccgaggcgcc cccttcgccg atggcgcggc gggcagcgtt
      241 gcgcgagtcc acgagtccca cggcgaccag ggcatcgacg acggacatcc cgaccgtcac
      301 cgctcccccg ggcagctctg ccgtggcgtc gcgcaaggtg gccgcgtcga gagctgtcac
      361 gtcacccttg ccgaacagcg cctcggaggc ggcctgcacc gatgccgtgg ccgccgcacc
      421 gtggacgagc gtcgtgacgt cggcagccag gaccttctgg gcagcgcgcc gaaacggctc
      481 gtgctccacc tggcgctcga gctcggcgat ctcctcgcgc gaccggtccg tgaagacctt
      541 cagcagggtg acgacggagg catcctcgac gttgacccag tactggtaga aggcgtaggg
      601 gctggtcatc tccggcgaga gccagatggc gttgccggcg ctcttgccga acttctctcc
      661 cgagctgtca gtgagcagcg gggtcgtgag caggtgcacg gacgcgccct ccacgcggtg
      721 gatgagatcc gagccggcgg tgaggttgcc ccactggtcg ttgcccccgg tctgcagggt
      781 gcagccgaag tcacggtaga gctggaggta gtccagcccc tgcagcagct ggtagctgaa
      841 ctcggtgtag gagatgccct cgtcgctgtt cagtcgagcg gcgatggcct ccttcttgac
      901 catctggttg acccggaagt gcttgcccac gtcgcgcagg aagtccagtg ccgacagcgg
      961 cgcggtccag tcgaggttgt tgacgaacac ggccgggttc tcaccgtcgg aggcgaggaa
     1021 cggctccacc tgccggcgga tgttggccac ccactgcgcg gtctgctcct tggtcttcag
     1081 cactcgctct gcgctcggcc gcgggtcacc gatgagtccg gtggacccgc ccacgaggca
     1141 gatcacccgg tgaccggccc gctggaggtg ccgcagcacg atcagctgga cgaggttgcc
     1201 gaagtgcagc gatggcgcgg tggggtcgaa gccgcaatac gccgtgatcg gcccgtcggc
     1261 gagtgccgtg cgcagcgcgg actcgtcggt ggactgcgcc accagccctc gccactgcag
     1321 ctcgtcgagg atgtcgctca cggtgcttcc cttctccacg tccgcgcctc gaccggatgc
     1381 cgggcgcccg ccacagcctg ccacggcccc ggccatccca cgcgcgcacg tccgtgcgcg
     1441 atgcggatgc cggtggcgga cggcatccgg cacactgtcg gccgtgatcg acaacgcggc
     1501 ctcgacggat cccctcacga cgttcgtcgc cggcctgccc aaggccgagc tgcacgtgca
     1561 ccacgtgggg tcggcctcac cgcgcatcgt cgccgacctc gccgctcgcc acccggacag
     1621 ccccgtgcct caggacccgg acgagctgga gcgcttcttc accttcaccg acttcgcgca
     1681 cttcatcgac gtgtacctcg cgaccgtggg gctgctgcgc accgacgagg acgtgcgcat
     1741 gctcacctac gaggtggccc gcgatctcgc ggcccagcag gtgcgctacg ccgagctgac
     1801 gatgacgccg tacacgtcgg tggtccgcgg catcccgatc gaggcgtaca ccgaggccat
     1861 cgaggacgca cgcattgccg ccgaacgtga ccacggcgtc gtcctgcgct ggatctacga
     1921 catcccgggt gagtccggcc tcgagtcggc cgacgccacg ttgacctacg ccctggacca
     1981 ccggccggag gcgctcgtcg gcttcggcct cggcgggccc gagatcggcg tgccacgacc
     2041 gcagttcgcg ccgcacttcg ccgccgcgcg gtccgccggg ctgcacagcg tgccgcactc
     2101 cggtgagtcc accggtcccc agtcggtgtg ggacgccctc acccacctcg gggcggaacg
     2161 gatcggccac gggacgacgt cagtgcagga tccccgcctg ctcgagcacc tcgccgagca
     2221 ccggatcccg ctggaggtgt gtccgacgag caacatcgcg acccgcgtcg tcgagagcct
     2281 cgacgtgcac cccatccgag cgatgcgcga tgccggcgtc gtcgtcaccg tcaacagcga
     2341 cgacccgccg atgttcggca ccacgctgaa ccgcgagtac gagatcgctg cccacctgct
     2401 cgacctcgac gagaacggtg tcgcggacct cgcccgggac gcggtcgacg cgtccttcgc
     2461 cccggctgac gtggccgcag ccctgcgcgc cgagatcgcc tcctacagcg aggagttcag
     2521 cgcagggcgg aggtgaccac gaccgcgccg gggccgaagg aggcgtcggc ccaggcctgg
     2581 atggacccgt cgtcgtggac cacggagacg agcaccggac cgcggtcgtc cgagcgtccc
     2641 gacgtgagca gcccgggcgt ggcgcgaagg gcgctctcca cggcatccca ctgcgcgtcg
     2701 ctgaggtcgg gcggtggcac gggggtcggg ctcggctgcg ccatcgtgtc gtagagggcc
     2761 aacgacacgg cactggtgac cgtcagcgtc gtcccgtcga aggtgccggt gaccgcgtag
     2821 gtgccccacc tggtgaccgg cgccccggcc tccgtctcgg agtcgggtgg gttgctcgcc
     2881 cagtcccagc cgacgagcgg tatgccctgg cactgcggcg gccaggactc ggcgaccggc
     2941 ccgaggcaca gctccggcgg gctgcccggc ttctcgatga ccgtgcccag gcccgtcacc
     3001 ggcgccgtgg ctgcgggcac cgcggtgacc atcggcacgg cctggctgct ggcagcaccc
     3061 ggggacggct cactcgagga actgccgcag gcggccagag gcagggcagc ggcggcgagg
     3121 acggcggcaa agaccatgcg catacgcgtc ggacgcatca accggccctg ccgttccggg
     3181 tcctggccac gcggtaggca ctcacggtgg gctcgccgtc gagccagaac cgccacgggt
     3241 acgccgtgcc gtcgccaccc gccccactga ccccgacccg cgggccggtg cgcaccgtcg
     3301 cggcatcgac cggtgtctcc ggcggctcga cccggccccc ggctcgcgct cccacgaggg
     3361 acagcccgtc gtgcgcacgg gtcaggccca gggtcatcgc caggttcgcc gggccgcggg
     3421 cctggtcacg ctcacgcacc cccggccgcc gctcagcggc cagctcgtgc cccgcgacca
     3481 cctcgccggc ccgcatcagc accgcggagg cggtacccgg ctggccggtc acgatgttgg
     3541 cgcagtggtg catgccgtag gtgaagtaga cgtacagccg accggccggt ccgaacatca
     3601 cctcggtccg cggcgatcga ccgcggaagg cgtgcgagcc cgggtccgcg cctccggcgt
     3661 acgcctcgac ctcggtcagg cgcacggtga cacccccacg ggtgacccgg catcccagga
     3721 ggtcgcgggc cacggtgagc acgtcacggg tgaagaactg cacgggcagg cgggtcgacg
     3781 tcaccccacc agtgtgtggc ctcgggagcg ggccggatgc cgtcagcgca ccgtcggctg
     3841 cgcgctcgcc caccgtcggt ggtcggtggc cgtcgcaacc acccgctcca gctgttccgc
     3901 gacccggacc ggagccgtgc ctccccgagc cgacctcgat gccatcgagc cctcgacgga
     3961 gagcacgtcg cgcacccccg gggtgaggtg cgggctgatc gcagtgaggt cgtcgtcggt
     4021 caggtcccac agctcgatgc cgcgctcctc gcacgcccgc acgcaggccc cggcgacctc
     4081 gtgggccacc cggaacggca cgccctcgcg cacgagccac tcggcgatgt ccgtcgccaa
     4141 cgagaacccc tgtggggcaa gggactccag ccgtgcggtg tcgaagacca tggtgtcgac
     4201 catgccgctg aaggccggca gcagcacctc gagggtgtcg acggcgtcga agaccggctc
     4261 cttgtcctcc tggaggtcgc ggttgtacgc caggggtagc gacttcagcg tcgtcatgag
     4321 cccggtgagg tcacccacga ggcggccggc cttgccgcgg gccagctcgg cgacgtccgg
     4381 gttcttcttc tggggcatga tgctcgaccc cgtggagtag gcgtcgtcca aggtgatgaa
     4441 cgagaactcc ttggtcgccc agagcacgac ctcctcggcc aggcgtgaga tgtcgacggc
     4501 ggtcatcgcg gcgacgaagg cgaactcggc gacgaagtca cgcgacgcgg tgccgtcgat
     4561 ggagttctcg accgaggagt cgaagccgag gtccgcggcc accgcctcgg ggtccagccc
     4621 gagggacgac cccgcgagcg ccccgctgcc gtacggcgag accgcggtgc ggacatccca
     4681 gtcgcgcagg cggtcgacgt cgcgcagcag cgcccaggcg tgcgcgagca ggtggtgcga
     4741 gagcagcacc ggctgcgcgt gctggaggtg ggtacgaccc ggcatcgcga ccccgaggtg
     4801 ccgcgtggac tgggccacga gggcgtcgac cacgtccagc gccaggccgg cgacgacccg
     4861 cgcgtgctca cgcaggtaca tccggaacag ggtggcgacc tggtcgttgc gggagcggcc
     4921 cgcccgcagc cggccgccga catccgcgcc ggcccgctcg atgagaccgc gctcgagcgc
     4981 ggtgtggacg tcctcgtcgc cgtgctcggg cacgaacgcc ccgctctcga cgtcgacccg
     5041 cagccggtcg agcgcgtcga gcatcgcctc gagggtggcg tcgtcgagca ggcccgcccc
     5101 gtgcaggacc cgggcgtggg cgcgggaccc cgcgaggtcg tgcagcgcca gtcgccagtc
     5161 gaagtgcgtc gacttcgaca gcgccgcgag ggcatccgcc ggaccgccgg cgaaacggcc
     5221 accccacagg gcggtgctgg caccctgtgc gggctgggac ggatctgacc gactggtcga
     5281 atctgcaccg gcggcggact ggctcgtcgg gtcggtcact gggggtccct tcgggtgtag
     5341 gccatgagtc ggtcgacgag gtcgcggctt gccgaggcgc cgcgcgtcac gaccatgatg
     5401 gtgtcgtccc cggcgatcgt gccgagcacc ccgtcgatcg tggcgtggtc gagcgccgag
     5461 gcgaggaagt tcgccgcgcc ggggggcgtg cgcaggatga cgaggttggc cgaggcctcg
     5521 gccgacacga gcagctcgtg gcagcgcgac tgaagtcggg tgccgcgctc ctcaccgtcg
     5581 gtggcggggc gcacggttcg gtcgccaccc tcccccggca cggcgtagac gaggctcttg
     5641 ccgacccgga cccgctcggc ccccacgtcg acgaggtcgc gcgacagggt ggcctgggtg
     5701 acctcgatgc cgtcagcggc gaggtggtcg agcagctcgg tctgcgagcg gaccggtgtc
     5761 gcggcgagga tctcgacgat ccgctgctgg cgggcagcac gcgacgtcga gatggtcatc
     5821 gaccgtcccc ggcacggtgc tgctccatca gccacgacag cagggccttc tgcgcgtgca
     5881 ggcggttctc ggcctcctgc cacaccaacg acgagggccc gtcgatgacg gaggcgtcga
     5941 cctcgtaccc gcggtaggcc ggcagacagt ggaggaagac ggcatccggc gccgccttcg
     6001 ccatgagcgc ctcgctgacc cggaagggtt cgaaggggct ggccgcaccg tggcgctgct
     6061 cgctctcggc ctccatgccc atcgagaccc acgtgtcggt ggcgacgacg tcggcgccgc
     6121 tcgccgcgac cacggggtcg tcggtgatga ggatggaccc accctgtgcg gcggcgtgac
     6181 gctgggcgcg ggccacgatg tcggcccggg gttggtgcga ggccggggtc gcgatccgca
     6241 cgtgcatccc ggccagggcg cacccgagca ggtacgagtg cgccatgttg ttcgcaccgt
     6301 caccgaggta ggtgaacgtc aggcccgcgg tggtgccctt gacctcggtg atcgtcagca
     6361 ggtcggcgag gatctggcac gggtggtggt catcggtgag ggcgttgacc accggcaccc
     6421 cggcctgcgc cgccatctcc tccagccggc tctgctcgaa ggtgcgccag acgatggctg
     6481 ccgactgcgg gccgaggatc cgcgcgacgt cggcgacgga ctcgcgctcg ccgaccccgg
     6541 ccagacgccc gtcgatgacc atcgggtagc cgccgaggtg cgccacggcc ccgacgaagg
     6601 agacctgggt gcgcagggtc ggcttgtcga acaggatcga caccgtgtgt ggcccggcca
     6661 ggcactggtc ggagaacggt tcggccttga gcgccagagc gcgctggagg acctcgtgct
     6721 gctctgctgc ggagaggtcg tcgtcggcga ggaggtgccg cagggtcatg gggtgtcctt
     6781 gagggtcgat gccgcgtcga gcagctcggg aagggcgtcg atgaacaggt cgagctcgtg
     6841 cgtggtgatg acgaggggcg ggacgaggcg cagcgcatcc ggtgcgacgg ggttgacgat
     6901 gaacccggcc tcgcgagcga gggcggccac cgtcgccgac acgtcagcgg ccagggtgat
     6961 ggctcgcatg agaccggcgc cccgtactcc tgtgacgagg gggtgcccga gcgccagcac
     7021 ccggcgctcg aggtgctcac cggccagtcg ggcgtgctcg atcaggccct gggactcgat
     7081 ggcgtcgagc accgccagcc cggcagcgca cgcgagcggg ttgcccccga acgtcgaccc
     7141 gtggttcccg ggcgtgagca tcgtgctcac tcgcgggccg aaggtgatca gcgccccgat
     7201 cggcactccc ccaccgagcc ccttggccag ggtgatggcg tcgggaacga ctcccgtgtg
     7261 ctggaaaccg aaccaggtcc cggtgcggcc gataccggtc tgcacctcgt cgaggatgag
     7321 cagcgcgccc acctcgtggg tcagctcgcg ggcgagccgc aggtaggcgc cgtcggcggg
     7381 gcggaccccg gcctcaccct ggatgggctc caggaccagc gccgacgtct gcgggccgac
     7441 ggccgcccgc agggccgcct catcacccca gggcacgtgc gtcaccccgg ggatgagcgg
     7501 ttcgaacggc tcgcggtagg ccggtttgtg ggtcagcgcc agcgcgccgg tggtccgccc
     7561 gtggaaggcg ccttccgcgg cgacgatgtc ggtgcggccg gtgcggcggg ccagcttgat
     7621 ggccgcctcg agggcctcgg tgccggagtt gcagaagaac accgcggaac cctccggtgc
     7681 cttcgcgagg tgcagcagct tctcggcgag ctcgacctgg gcgacggtgg tgaagaagtt
     7741 cgacacgtgg acgagctgcc cggcctgctt ggacaccgcc gcgacgaggg cagggtggtt
     7801 gtggcccagg gcgttgacgg cgatcccgcc gacgaggtcg aggtagcgcc ggccgtcgac
     7861 gtcccagacc caggccccgt cgccgtggtc gaggacgagc tgggggcgcc cgaacaggtt
     7921 cagcagcgag ccctcgtagc gctcgatcca ggccgcggtg gcctcgccgg atgccgtggt
     7981 ggcggatgcc gtggacgcgt cactcatcgt ggacctcccg atcgggcaga accatggtgc
     8041 cgataccctc ggacgtgaac acctcgagca gcaggctgtg cggctggcgc ccgtcgacga
     8101 cgtgcgcctg gggcaccccg gcctcgacgg cccggatgca ggcctcgagc ttggggatca
     8161 tcccggcgtc gacggtctct agcaggcgac gcgccctggt caccgacagc tcggagagca
     8221 ggctcgccct gtcgggccag ttcgcgtaga ccccctcgac gtcggtgagc acgacgagct
     8281 tgcgcgcccc cagggcggcc gcgagtgcag cggcagcggt gtcggcgttg acgttgagca
     8341 cctggccgtc ggtgtcgaga tcgggggcga tcgtcgacac caccgggatg cggcctgcgt
     8401 cgagcaggtc caggacagcg gtcgggtcga cctgcacgac gtcgccgacg aggccgatgt
     8461 cgtgctcctc gccgtcgacg acgacgccgc gacgacgcgc accgaacagc ccggcatcct
     8521 cacccgagag ccccacggcg accgggccgt gctggttgag caggccgacg agctcacgac
     8581 ccacctggcc ggtgagcacc atccgcacga cgtccatgac ctcgggcgtc gtgacccgaa
     8641 gcccgccctt gaactcgctc gcaaggccga ggcggtcgag catggccttg atctgcggac
     8701 caccgccgtg aacgaccacc gggcgcaggc cggcgaagcg caggaacgcg atgtcctggg
     8761 cgaatgcggc cttcagggcg tcgtcgatca tcgcgttgcc gccgtacttc acgacgacga
     8821 gggcacctcg gaagcgttcg agccagggca gcgcctcgac gagcgtgctc gccttggact
     8881 gggcgacacg cagggcggcg gcgtcgatgg cgccgcgcag agcctggtcg gggggtggca
     8941 tgtgggtgtc cttcggtgcg atctggtggc tcaggtggag tacgcggagt tctcgtggac
     9001 gtagtcgtgg gtcaggtcgt tggtccacac cgtcgcggtg gccgacccgg cgtgcaggtc
     9061 gacgacgacg cggacctcgc gggcggtgag gtcgaccagg gatcggtcct cgccgactcc
     9121 cccggcccga cagaccatca gcccgttcat cgacacgtcc agctcggcag ggtcgaacag
     9181 cgcctgcgtg gtgccgacgg cggccagcac ccggccccaa ttggggtcgt tgccgaacac
     9241 ggcgcacttg aagaggttgt tgcgcgcgac ggaacgagcg acctcgagcg cgtcgtcctc
     9301 ggatgccgcc ccggtcacct cgatcgcgat gtcgtggtgc gcaccctcgg cgtcggcgat
     9361 cagctgacgg gcgaggtcag cgcacacctg cgtcacggca tccgtgaggt ctgcgagggc
     9421 caccgtcgtg cccgaggcac ccgaggccat gagcagcacc gtgtcgttgg tcgacatgca
     9481 gccgtcggag tcgatgcgat cgaacgtcat cgcggtggcc gagcgcagga ctgcgtcgag
     9541 gtcgtcggcg acgtgggcgc cctcgatgac ggcgtcggtc gtgatgacga cgagcatcgt
     9601 cgcgagcgcc ggggcgagca tgccggcgcc cttggccatg cccccgaccg accagccgtc
     9661 gcggacagcc gtcgcggtct tcggcaccga gtcggtcgtc atgatggccg tcgccgctgc
     9721 cgggccgccg tcgacggaga gttcctcgac agccgcctcg acgccgggca gcagcagccc
     9781 catgggcagg cgttcaccga tcagcccggt cgagcagacg acgacgtcgc cggccgccac
     9841 accgagcacc tcaccgacgt gctcggccgt gcggtgggtg tcctggaagc cgggggcacc
     9901 ggtgcaggcg ttggcgccac cactgttgag cacgacggca tccgcccgtc cgtcgctgat
     9961 gacggtgcgt gaccaggtga ccggcgcggc ctccacgcgg ttggtcgtga agaccgcggc
    10021 cgcgtggtgg tgcgggccgt cgttgacgac gagcgccaca tcgggcttgc cggaggcctt
    10081 gaggcccgcg gtgacacccg aggcccgaaa gccgaggggg gtggtgacgg acatcaggag
    10141 gatcctccgg tcaacgaggt tggggtgatg atgagcccgg cacggcgcca ggcgtgggcc
    10201 gcttcgtcgg tctgcccggc cggaacgagc acccagtcgg tgtcgaaggt cgagaaggcg
    10261 aggacgctga tgtcggcgtc gacgagcggt tccacgacct cggcgaccac cccgaccgat
    10321 ccgaacgaca ggggcccggc cacctcgacg acccggaacg ggccctccgt gcgcactccc
    10381 gcaggaacgg ttgccgtgag gcacacgacg gaggtctcgt ctggagttct ggtcagtgac
    10441 gcgaaaggcc ctgcagccca gtcgaactcg ggcagctgcc cgccgtcgag gcgcacgatg
    10501 gagacgtcct cggggtgctc catcaggtga cgtggcatga cgctcacggc gccacccccg
    10561 cgacggggag accggtggcc tcgggcaggc ccagggcgag gttgaggcac tggacggcgg
    10621 caccggccgt gcccttggtg aggttgtcga tcgctgcgac gacgacgacg cgcccgaccc
    10681 tctcgtcgag ggtgacctgc aggtggaccg cgttgctgcc gagcacgctg ccggtgctcg
    10741 gccagcgacc cggcggcagg aggtgcacga agggttcgtc ggcgtaggcg gtcgcccagg
    10801 ccgcgcggac ggcatccgcc gtcgtcccgg gacgcacccg ggcggtgcac gtcgcgagga
    10861 tgccgcgggg catcggcgcg agggtcgggg tgaacgagac cgtgaccggg ccggcgcctg
    10921 cggcggtgag gttctgctcg atctcggggg tgtgccggtg ggtgccgccg acgccgtagg
    10981 gcgacatcgc tcccatgacc tcggcgccga gcaggtgtgg cttgagcgcc ttgcccgccc
    11041 ccgacgtgcc ggatgccgcg acgacggtga tgtcgtcggc ctcgaggacg cccgcgacga
    11101 gtccgggagc cagggccagg gagacggccg tggggtagca gccggggacc gcgatgcggg
    11161 tggcgccggc gagggccagc cgctgcgggc catcggccgt cgcgagctcg ggcaggccgt
    11221 agggccacga tcccgcgtac ggcgtgtcgt agaaggtgtc ccacgcggcg gcgtcagcga
    11281 ggcggaagtc ggcgccgcag tcgatgatga tgacgtcgtc ggggagctgc gcggccaggg
    11341 ccgccgagtg cccgtggggc agcgcgagca cgacggcgtc attgccggcg aggacgtcgg
    11401 gcgtggtgtc ctcgagaacc cggtcggcca gcggggtgag gtgcgggtgg atcgacccca
    11461 gggtctgccc ggcgttcgac gaggcggtga gcgccccgac ctccagctcg gggtgctgga
    11521 ggaggaggcg gaccacctct cccccggcat agccactggc accggccact gcaacccgct
    11581 tcatgctgca tgactataca gagtcccgcg actctatgca caacatgccc tcgagagggg
    11641 tcaggcgagg gggtcgtcgg gggtgtacgg cacgaggccc agggtgcggt ctcgctcccc
    11701 cagcgtgtcg gcccgctccc gcagcgactc caccgtgtcg accccggcgc ggacgaaccg
    11761 ccccgaccag gcgtcgagct cacccgaggc cagggcgagc acgagatcgg tgacgtcctc
    11821 gggcgcggtc cactcggtgc gcccgacgtg ggcgtgcatg gcctgcgtca tgtcggtgcg
    11881 cacgaccccg ggcatgaggt cgaacgcccg gatgccgcga gcccacccgg cgaggtgggt
    11941 cgaccccgtg atcctcgcca acgccgtctt gctgacgttg taggcgctcg cgtccagccc
    12001 cgggcgcagc gcggcaccgc tgttgatgtt gatgatccgc ccaccgccct ggtgcagcat
    12061 ggcctccccg acgagccggg tcatgaggta cgccccgagc acgttgacct cctggacctg
    12121 ccaccactcg tcggggtcgg aggcgaggac gtcgacctcg gtgtcgatga cgccagcgtt
    12181 gttgacgagc acgtcgatgc gcccctggcg ctcgaggatc tccgcgaccc aggcgctgac
    12241 cgccgcccga tcgctcaccg gggcggttgc cgtggacccg cgctcgacgg agtacccggc
    12301 atcctccaag gcgtcggcga cgtggcggcc gatgccgcgc gacgcgccgg tgaccaccgc
    12361 gacgaggtgc ccgccccgac cgtcgtcggt gacgggttcg ggggtcacct caccgtcgtc
    12421 gtcgaccggc acctcgacgg gggtgctcac gcgcgctgca ccgctccgac ggcctgcgcc
    12481 gccgccgcca gggcacggtc acgcaggccg ctcacctcgt cggtggtcag cgtgcggtcc
    12541 ggcgcccgga aggtgagccg gtaggccagg gacttgcgcc cctcgccgac ctggtcgccg
    12601 cggtagacgt cgaacagggt gagggactcc aaggtctccc cggcaccacc gcgcagcgcc
    12661 tgcgccaccc gtgccgccgg gacgtcctcg tcgatgacga cggcgacgtc actctgcgcg
    12721 agcgggaagg tgtgcaccgt gcgggcctgt ggggtccctt ccgaggccgt cgtcagcacg
    12781 tcgaggtcga gctcgccgcc gaccgcccgg gccggcagtc cgagggcggc cacggccttc
    12841 gggtgcagct caccgacgtg accgacgagg gtcccgtcgc tcagcgtgat ccgggcacag
    12901 cggccggggt ggaacggggc cgcggtgtcg gccacgacct cgatcgccac cccgagggcc
    12961 tcaccgaggg tgcgcgcgag gtcgacgacg tcggtgacgt cggccggacg gcccggcccc
    13021 caccagccgg cgcgctcgcg ctcgccggcg agcaggaagg cgacgtggcg cggctgcggc
    13081 ggcacggcat cccgaatcgc cgccagggtc tcggcggagg ggtggatgcc gacgtcctcg
    13141 gtgggcgccg ccgcctgcgg cccgtcgagc gcgacgacga gccccagctc gaacaggccg
    13201 acgtcgcggc tgccgcgggc gacgttgcgc cgcagggcgt cgacgagcgt cgcgagcagg
    13261 cgggtgcgca tgagcggtgc ctcctcggac aacgggttgg ccaggcgcac cgtgcgagcc
    13321 acctcggcct cgacgtcgag ccccaggtcg gcgaagcggt cggcaccgac gaacggggcg
    13381 ctccacacct cggacaagcc ctgtccggcc aggacgtcgg cgacgaggcg ccgggtgcgc
    13441 tgggcgtggg tgagccccgc gcctccgggc ggtgtcggca cgatcgacgg gatgccgtcg
    13501 tacccggcga tgcgggcaac ctcctcgacg aggtcctcgg cggtcgtgag atcagggcgc
    13561 cacgtcggcg ggatgaccgt cagcaggtga ccctcccgag tcaccgaaca gccgatggac
    13621 gacaggatcg acacgacctc gctctcgagg tagacgatcc cgacgatctt ggacgggaag
    13681 tcgacgtgca tcgtcacggg gtccggtgcc gccgggctgc cgacgtcggt gacctcgggg
    13741 tcggccactc ccccgccgtg ctcgacgagg agatccacgg cgagctgggc tgcggcggcg
    13801 gcgagcgccg ggtcgacgcc tcgctcgaac cggcgcgagg cctcgctggg cagcttgtgg
    13861 gcccgagcgg tgcgggcgat gctcaccgcg tcgaagtggg ccgcctcgat gagcacgttc
    13921 cgggtggtcg caccgacctc ggtctccgcc ccacccatca cgccggcgat tgcgatcgcg
    13981 cgctcgccgt cgtggtcggt gatcagcagg tcgccctcac ccaggctgcg ctcggcgtcg
    14041 tcgagcgtgg tgagccgttc gccggcccgg gcccgccgca ccacgatggg cccctggagg
    14101 gtgtcgaggt cgaaggcgtg cagcggctgg ccgagcccga gcatgacgta gttcgtcacg
    14161 tcgaccggca acgagatggg gcgcatgccg gcctcggtga gccgggtcgc catccagcgc
    14221 ggcgtcggcg cggagacgtc gatgttccgc acgatgcggg cgacgtagcg gtcgcaaccg
    14281 gcccggcccc ggatcggggc ctggtcgtcg aggacgacgg ggaagccgcc ctcggtcgcg
    14341 gcgggggccg cggcatccag agcggtgacc gggtcggtga aggcggcccg ggtggcgtgc
    14401 ccgtactcgc gggcgatgcc gcgcaccgag aagcagtagc cgcggtcggg cgtgacgttg
    14461 acctcgacgg tctcgcggtc gagcccgagc agcgggagcg cgtccgtgcc gggggtgagc
    14521 gtggccaggg tggcctcgtc gccggcgaac ctcttcgtca ggacgaggat gccgtcgtgg
    14581 tcgtccccga tccccagctc gcgctcggag cagatcatcc cggccgagac gtggccgtag
    14641 gtcttgcgcg cgctgacggt gagcggcccc atcggggtgg tgagcacgcc gccgggcagg
    14701 atgacgacga cgaggtcccc ggcatcgaag ttgtgcgcac cgcacacgac accctggggc
    14761 tctccggtgc cgttggcgtc accgacgtcg acggtgcacc agttgatcgt cttgccgttc
    14821 ttctgcggct cgggggtctt ggtcagcacc cggccgacga cgagggggcc ggtgacacca
    14881 ccggtgtgca ggccctcctc ctcgagaccc acgcgcacga ggtcggcggc gatctgctca
    14941 cccgtggcgt cgtggggaac cgcggcgtac tcgcgcagcc agtcgatggg aacgcgcatc
    15001 agatctccat cccgaactgt gcgtcgaagc gcacgtcacc ttcgatcatg tcccgcatgt
    15061 cgctgacccc ggtgcggaac atcagggcgc ggtcgatccc catgccgaac gcgaaccccg
    15121 tgtagcggtc ggggtcgacc ccgcaggccg tcagcacgtt gcggttgacc atcccgcagc
    15181 caccccactc gatccagccg gtgcccttgc acgtgcggca ggccgggtcc tcgccacggc
    15241 agacgaagca gcgcaggtcc atctcggcgc tgggctcggt gaaggggaag tagctcgggc
    15301 gcagtcgtgt cgtgatgccc tcaccgaaca gctcggtggc gaggcggtcg agggtgccct
    15361 tgaggtgggc catcgtgatg ccctcgtcga cggccagtcc ctccacctgg tggaaggccg
    15421 gcatgtgcgt ggcgtcgagc tcgtcggtgc ggaacacccg accgatcgcc gcgacgtaga
    15481 ccggcacgcc acgctcgagc agggtgcggg cctgcaccgg cgaggtgtgg gtgcgcagca
    15541 cgagcccacc ctcgggcggg tcgacgtaga aggtgtcctg catctggcgc gccgggtggt
    15601 cggcatcgaa gttcagggcg tcgaagttga accactcggc ctcgatctcg gggccctcgg
    15661 cgatctccca gcccatgccg acgaacaggt cggcgatgcg ctggctcatc agctcgatcg
    15721 ggtgccggcg acccagtggg cggcgacccg agggcagcgt cacgtcaacc gcctcgtcgg
    15781 tgaggatccg ctcgtcacgc tcgatctcga gctcaccctg acgggccgtg aacgcctgcg
    15841 ccacgcgccc gcgagcctgc ccgactcgct tgccggcctc ggccttggcg ctcggcggca
    15901 gggctccgat ctctcgattg gccagggcca gggggctctt gtccccctgg tgcgcggtgc
    15961 gggcggcctt gagctcgtcg agcgaggtcg cggccgcagc cgcagcgagg gcctcggtga
    16021 cggcctcctc gatggcgtgc ggatccagcg cggcgacctc gacggggtcg tagctggtgt
    16081 tcggtccaga catggggtcc acttcagggc aggttggggt ggtcttcggg cggccccgag
    16141 tctaggttgc ggatgccgtg gctcgccgca cggataccgc cgtgagaccc agcgcggcca
    16201 ccacgacgcc cacggcggcc accacgacga agccggcccc gggcccgccc cggtcgatgg
    16261 ccaagccagc caagggtgcc ccgacggccc caccggtggt catgaacgag ccgtgccagc
    16321 ccatggcctc gccgcgcacc tgcgcgggca cgatccggct ggcctggtcg atggtcgccg
    16381 tgatcgtcgg cgcacacagc aggccggcga cgaagccgga cgcggccagc tggacggtcg
    16441 aggaggccag tgccatgggg aaggtggcgg ccgccaagcc ggcgagcagc cagaacgccg
    16501 agatgggccg gtgcagcgcc ccgtagagca agccaccgat gagtgacccc agcccccaca
    16561 ccgcgagcac gagaccgatc tgcccctccg cggcgatgtc gcgcatgacg gcgatgatcg
    16621 agatgtccga gcccgccagc acgacggtgg tcgctgtggc agccaggcac acggcgagaa
    16681 acggcagccg gaaccacgtc cggctgctgg cgcgcgcggt cccgaccacc gcttccccct
    16741 caccgcgcaa cggcgggtcg acgacgtaca tgaggatgcc tgcgcccacg ccgagcatct
    16801 ggatcgtgaa caacacccac gaggtgtccc acaccgtggc cgcccagaca gcaaccgccg
    16861 gggcgacgat gaacgacagc tcgaccgcca cggcatccaa cgagatcgcc gtccggcgct
    16921 cctcctcccc gactgctgcg atgacggcct gtcgggtgat ggagaacgtc gggatgacga
    16981 acaggccggc cagcaccgcg aggcccagca gcagcaggta gtcgacgaag ggggcgatgc
    17041 tccagcacac gagtgcgacg aggatcgagg gcagcaccac gcgccgcagg ccgtacctgt
    17101 cgagcaagcg cccgcgccag ggcccgctga cggcgatggc gaccgttgcc gcggcggcaa
    17161 gcacgcccgc cgcggtgtag ctgcgatcca gggacgacac gacgtggatg gtcagcagga
    17221 cggtgctcgc gaacaccggc atccgcacga gggtgcccag gacgagtgcg gttcgcagcg
    17281 gcttgctccg aagcgcccgc cggtacgggg cgaaggacac ggcgggcacc gggacactgt
    17341 gtcaccgaat ggccagactg acgactcgga ggcactgaca gcgcgccggc gccgagggtt
    17401 gggtcccgtc cgggacgagg aggaccgccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn ccacgagatg tcccgcgact tctggtcacc ggtggagccg ctcgtcggcg
    17581 aggacgtgcc cttcccgtac ttctcgccgt ggacagccgt cggcgcgctg ggggtgcggc
    17641 tgttctccgt cactcctttc caggtgctgg ccgtcttcgc cgtggtgtcc cccctggtcc
    17701 tggtccactc gctgcaccgc ctcgtgcgcg ccgtgagtcc cgctccgtgg gcccccgttg
    17761 ccgtgctggc ggcactcctg acggtctggg ggaccagagc gttcttctgg agcggattcc
    17821 tcagcctcaa caccctcgtc gtcgggtcct cgtggcccag cgtcctcgcg accgcgttct
    17881 ggttctacct gtgggccgcc gtctggacca gcacccggat gactcggggg ttgatcgcga
    17941 cgctgctcgt cgttcccggt gtgctgctgt tgttccaccc gttcacgttc ctcagcgctg
    18001 ccacggcctg cgccatcacg gtgctcctgc gctggccacg acagcggtgg ctctcgggcg
    18061 cagtgccgtc cttgatctcg gtcggcctgg ccgccctctg gccgtggatc tccctggggg
    18121 atcttctcga caacccacgc gggttcgacg ggttccacgt gttcttctac gacgacgtga
    18181 tggcgaagtt cgggctcctc ctcctcacga tcccggccat cgtccttcgg ctcggtcgac
    18241 agcgcaccga ccctcttggt tggtcggtgc tcgcaggcgt cggcatctgg gccgccggag
    18301 gcgtgctcca cgtcgagtcc ctgggcaggg tgattcccct cgcgtcaacg gccgcggcgg
    18361 tggccctcgg cgtggccgcg gccgaggcac tgcaacccgc ggatgccggc tcgacaggca
    18421 ccgctcgacc ccgcggcgcg tcggccgcgc gcattgtcgt cgtgctggcc tgggtcgtcg
    18481 ccgtggctgt gggcagcatg acccagtcca acgcgtggag ccgcatcacc gtcgacgacg
    18541 cggcccgtcg caaccccgat gacggcaccc acctcatctc gccgtatccg gtcgtcaccg
    18601 atctgtgggc ctcgatcccg tccggttcgg tcgtcgtggc gagggacccg cgagtcgcgc
    18661 ggcagctacc ggcctccgga ctgttctcgg tggcgcccca gtggcccagc ccaggagtca
    18721 cggacacccc ccggcgtgtc gatgacgagg cggcgatcct cagtgccaag acgtctccag
    18781 aactgcgcga ggaactgctg acccgctatg acgtcggctg gatcgtgtgg ccggacgcca
    18841 agaagacacc gacctggttg gctgagctcg gcgtgccgct cgacagcgtg gccggtcaga
    18901 cgctgtacga cgtgcgctga cccgcccggg tcggctgtcg tgctcggttc agatgtcgcg
    18961 gtcggggaag gtcgcgcgca gctgagcccc gccccctggt gcctgctcgg cgacgatcac
    19021 cccgccatgg gcctcgacca ggccccgcac gaggtagagc ccgagcccgg tgccctgctt
    19081 cgcgccgtcc tgccagaacc gggagaacgc cagctcacgg tgctgcgggt cgatgccgtc
    19141 gccgtcatcg gccacgagga cgtcgaccgc tggcgcgccg tcgagctcac cggcccgtgc
    19201 ggccaggcga accgtgcccc gaccgtggat gacggcgttc tcgacgaggt tggcgacgat
    19261 ctgctcgaaa cggtccggat cggcccacac cggaccaagg tcgtccggca cgtcgacgac
    19321 gaagcgctcg gcgtcggcat ccgcgggggc atccgcaccc gacgcgatgc gacgctgggc
    19381 gtgcgcccga agcacggcgg cgacgtccag gggctgcggg tgcacggtca ggtgccgcgt
    19441 gttgatccgg gagacgtcga gcagttcggt gatcaggcgg gtgacgcggt cggcgtcggc
    19501 ctcgatggtc tcgagcatga ggcgcttctg gtcatcggtc agccggtccc agttgcgcag
    19561 caacgtcgcg gagaaaccct tgacgccggt gagcggcgaa cgcagctcgt gcgccaccgt
    19621 ggtgagcagg gccgccgtct cgttctcggc gcgacgccgc gactccgcgt cccgaagccc
    19681 cagcaccacc gcctccacgg gctcccccgg cgccggacgc acgtacttcg cggtgacgag
    19741 gacctcgcgc cccgagggca ggaccagcag gcgctcgcgg tgtcccttgc ggctggccag
    19801 accgttccac gggtcggtga gctcccacca cgaggctccc gcgtggtcct ggagcggcag
    19861 gacctcccgg acgtcaccgc cgatggcgtc ctcgacgcgc acgccggtga tgcgctcggc
    19921 gcaggagttc atgaaacgca ccgtgcgctc gggcccgacc gccgcgacga ggccgtcggg
    19981 caggaactcg agaccgttga cgacgaggtc atcggggacc cggaaggggt gcgccatgcg
    20041 gggcacgtta ccccgcggtg gtgacaccct cgcgccgcgg tcgctgaccc gccgacgcgt
    20101 acaggcagac cgtcgccgcc atggcgaggt tgagcgattc ggcgcggccg tggatcggca
    20161 cccggacgac ctcgtcgcag gcgtcgcgaa cctcgtcggg caacccccag gcctcgttgc
    20221 ccatcaccca gcagtgcggc gccgtgaggt cggcatccgg aaggagcgtg gcgcccgccc
    20281 cgtctgcggc cagggtgcgg atgccgtgag cacgcaacgt ctccagcgtc gcctcgacgt
    20341 cgagacccgt caccacgggc aggtggaaca acgaaccggc cgtggagcgc acgaccttcg
    20401 aggagtggat gtcgaccgac gcactggaga cgagcacggc gtcggcgccc gccgcatcgg
    20461 caccgcggat gacggtgccg gcgttgccgg ggtcacgcac gttcgtcagc agcacgagca
    20521 ggcgtgggtg ggcgtcgagg accgccgcga gagcgtcagc aggccccacg gcatccgccc
    20581 cttcgtcact gtccacgacg tcgaccacgg cgaggacccc ttggggggcg tcggtctcgg
    20641 ccatcgccgc gaggacctcg gcgctcacct cgtgcaccca gaggttggcg gctcgggctg
    20701 gctccacgat gtcgagggca tagcgctcga gggcttcaga ggtgacgtag acgtcacgaa
    20761 cccgggctgc ggcatacctc accgcctccc ggaccccctg gggcccttcg acggcgaagc
    20821 ggccggtgcg ctcacgcacc gaccgcttcg acagcgctcg aaccgaccgg acccgctcgg
    20881 cgcggggatt ggtcagcatc ggcaggacgc tcaggcgtca gccttggcgg gctcacgagc
    20941 cggctggttg gccttggaga tctcgacgag cgcggtgaac gcggcggcgt cgttgacggc
    21001 cagctcggcc agcatgcgac ggtcgacctc gacctcagcg gccttcaggc cctggatgaa
    21061 ccggttgtag gtcatgccgt tcgcgcgggc cgcagcgttg atgcgctgga tccacaggcg
    21121 acggaagtca cccttgcggg cgcgacggtc gcggtagctg tagcccaggc tgtgctggac
    21181 ctgctccttg gccttgcggt acaggcgcga gcgctgcccg cggtaaccgc tggcccgctc
    21241 gaggacaacc cggcgcttct tctgggcgtt gaccgcccgc ttcacgcgtg ccacgtgagt
    21301 actccttgct tctctacgaa aatctggggg tggacggcgt cagcggccga ggagcttctt
    21361 gaccttcttg aggtcgccct tggacacgag aacgtccttg ctgagggcgc gagccttctg
    21421 cggggtcttc tcgtggaact tgtggacgtg accggcctgc tcgcgcatga ccttgccgct
    21481 gccggtgacc cggaagcgct tcttggcgcc gctgtgcgtc ttgttcttcg gcatggtgcc
    21541 gatctccttg cgttggtcgt tccagcgtgt ctgtggtgct ggtgcttcgg tggtgcagtc
    21601 ttcggggccg gtcaggcctt gggcttcggg gtcgcccgcg gcttcggggc ggccttcggc
    21661 ttcggtgccg cggcctgcgg cttgggggcc gcggtcggct tggcggcagc cggcgtgggt
    21721 gccggtgcag cggcgggtgc ctcgacctcg gctgccaggg ccggggccgg ggcgtcttcg
    21781 gcggcagggg cttcggccac gggggcctcg acgaccgggg cggcctcgac gacggcggcg
    21841 ggagccacga acgcctcggc ctcgcggacc tcggcctcga tgctctgctc gggtgcactg
    21901 gctgctgcgc tgccctcgtc acgacggcga cgctgctcgg ccatggcctg cgccttcttc
    21961 ttcgtcggcg cgatgaccat gaccatgttg cggccgtcct gcttgggcga ggactccacg
    22021 atgccgagct cggcgatgtc ctgcgccagt cgctggagca ggcggtagcc cagttcggga
    22081 cgggactgct cccgaccgcg gaacatgatc gtcaccttga ccttgtcgcc acccttgagg
    22141 aaccgctcga cgtggccctt cttggtgccg tagtcgtgcg ggtcgatctt cgggcggagc
    22201 ttgatctcct tgatgaccgt gttgacctgg ttcttgcgcg cttcgcgtgc cttcatggcg
    22261 gcttcgtact tgaacttgcc gaagtccatg agcttggcaa ccggcggctt ggccatcggt
    22321 gccacctcga cgaggtcaag gtctgcctcc gcggcgaggc gcagagcgtc ctcgacgcgc
    22381 acgatgccga cctgctcgcc gttcgggccg accaaccgca cctcgggaac ccggatgcgg
    22441 tcgttgatgc gtggctcgct gatgtgcttc tccttcgatc acgatcgttg cgctgatgcc
    22501 ggtgggcacg agaaaggctc ctcgcgacac gtgcacgagg agcctggatc ttccgattcc
    22561 gaaccgatcc gccacacagc cgatgccgtc gccacaccct cgcgggttcg gcgcgaccac
    22621 agtgcgtcgc agacctgttg gacccggcaa cctgatggtt gacgcgggtg gaagctgggc
    22681 ttctcttgca caccccccgt aagggaggtc ggtcagcggt caacactacc agcgacccgg
    22741 catccgccga aatcggctcg tggtcagcgg atgcggaagg ccagcccgtc gatgcgcgcc
    22801 cgaagctcac cgtcggtcgc gagcttctcg ccgacccggg tcgccacggc ccgcacggcg
    22861 tcggggccca gaccggggcg aaggtcgagc acgacaccga gcaccccctc gccgcggggg
    22921 ttgccctcct cgatctcgga tgccgtgacg tcgggttcgt cgcgcagcgc ggcggtgacg
    22981 ctggcggcga cgaacgggtc ggtgtgcgcc ggcacccacc gacgctgttg ggccagtgcc
    23041 cacaccatcg aggggcgcag gacccgggtg gccgggcctg cgacgtcgac gacgatgacc
    23101 tcgcacccct cggacacggc cgcctggccg gcccgcgcag gggtcaccgg caccggacgg
    23161 gcctcggggt cccaggcggc gacggcctcg atgccggaga aaaccggcag ggcacgcatg
    23221 ccgtcggggg cgacgagcgt cacggccgcg aggtcgacct ggctgtcgac ggcgtgctcc
    23281 cccgaggtgt cgacctcggt gggttcggcg acgatcggca cgaggaagcg acccgcctcg
    23341 acggccgcca tgagggcgac gtcgtcgccg ggggcggcca gtgcggcgag cagcgccgga
    23401 tcggccgcgc cggtgtcggt gtcgaacccg ctcggcgaga gctcacgagc ggcccacggc
    23461 aggtccgcgg agtcggtggc ctcgcccgcg ggtgcgtgca gggggtggtg ctcgtgctcg
    23521 cgcgggttgg tcatgcccgg ccggcgacgt cgagcgcctg gggcagcgtg aaggccccgc
    23581 ggtacagggc cgagccgatg atcgcgccct cgaccccgtc acccacgaga ccacggatgg
    23641 ccacgatgtc ctcgatggtg gacaccccac cggaggcgat gacgggacgg tcggtgcggg
    23701 cacacacgtc ccgcagcagg tcgaggttcg ggccctggag catcccgtcc ttggcgacgt
    23761 cggtgatgac gtagcgggcg cacccctcgg cgtcgaggcg cgcgagggtc tcccagaggt
    23821 cgccgccctc ctgcgtccag ccgcgggcgg ccagggtcgt gccgcgcacg tcgaggccga
    23881 ccgcgacccg gtcaccgtgc tccgcgatgg cccgcgcggt ccattcgggg ttctccagcg
    23941 ccgccgtgcc gacgttgacc cgacggcatc cggtcgcgag cgcgcgctcg aggctctctg
    24001 catcgcggat accgccgctc atctcgacgt cgatgtcgag cgttccgacg atgctcgcga
    24061 gcaggtccgc gttggagccg cgaccgaagg cagcgtcgag gtcgacgagg tgcagccact
    24121 gcgccccctg ctcctgccag gccaacgcgg cctgcagcgg gtcaccgaag tcaccgccgg
    24181 tgcctgccac cccctgcacg agctggacgg cccgcccgcc gacgacgtcg acggcaggca
    24241 ggagctgcag gatgggctgg ctggtcacga ggggtcctcc gcggcgtggt cgagcagatc
    24301 tgggggcggt gcccagtctg ccggatcggg tcgtaggggt cggaccccgt tcgtcagagg
    24361 gaacgcaccc agttgcgcag cagggccagc ccggcctcgc cggacttctc cgggtggaac
    24421 tgcgtcgccg tcagggggcc gttctcgacc gcggcgatga accgctcgcc gtgggtcgcc
    24481 cacgtgacgc ggggtgccac tgccgcgaac gggccgtccg aggcctcgag ggcccaggtg
    24541 tgggcggcgt aggagtgcac gaagtagaac cgctcgtcgg cgatgccctc gaacagcacg
    24601 ctccccggtg cgacctcgac ggcggaccag cccatgtgcg gcaccaccgg ggcccgcagc
    24661 cgctccacgg tgcccggcca ctcccccacc ccggccaggg gcgtcgcgct gggctcgctc
    24721 gacccctcga acatcacctg catgccgaca cagacgccga ggacgggacg accacctgac
    24781 aggcgctggt cgatgatccg cggcccgtcg gcggcaatga gcccggccat gcaggcgtgg
    24841 aagttgccga cgcccggcac gaagagcccg tcggcagcca gggcggcgtc acggtcaccg
    24901 gtgaggctga cctcggcacc gacgtgctcc agcgcgcgca cggcgctgcg gatgttgccg
    24961 ctgccgtagt cgagcacgac gacacgggca ctcatcggcc actcacctcg gcctggtggg
    25021 cggcctcctc ggccatgagg cggtcgaagg tcatgacgcc gcgcaccgag ggctcgacgt
    25081 cgtgggcgcc gtcgctgtcg ccgcgcacca gcaggtcgcg gccgggnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25201 nnnnnnnnnn nnnnnnnnnn nnnnnngctc gacgtcgtgg gcgccgtcgc tgtcgccgcg
    25261 caccagcagg tcgcggccgg gcagcccgag caggccgaga acggtcacga gcaggtcgac
    25321 cggtgcgccg gtccctgagc gcagcacctc ggccacgacc tcctcgcgca cccgcccgga
    25381 ggcgccggcc acggagagca gcagcggcaa ccgcaggggg gtgagggagg gcgtcgggac
    25441 gaggccggcg cccggctccc acacgagcca gtggatgtgc gagcgcagac cgggtggctg
    25501 aaccgtgatg gcagcccgac cgtgcacgtc gaagaacccc agcgcgggtc gtgcccgacg
    25561 cggcgcgggc cgggccgcga gcagttcgag gccgacgtcg tagggggcca cgctgcgggc
    25621 gcgttcctcg gccagggtga tgccggtcca ggccccgaac ggggcgacgg tcaccgccac
    25681 cagacctcgt cgcagccacc ggttgacggc ggccggaggc tggcgcacga gaaccagccc
    25741 gtgcccctcc cccgtgcgcg cgaccccggc cacgggctag agcgcaccct tcgcgctcgg
    25801 gatcccctcg accctggggt cgcgggcgac cgcggcacgc agagcacgcg ccagcgcctt
    25861 gaactgggcc tcgacgacgt ggtgggggtc gcggccgccg agcacccgca cgtgcagggc
    25921 gatctgggcg tggaaggcga aggactcgag cacgtgacgg gtgagcgagc ccacgtagtg
    25981 gccgccgatg acgacgtact gctggccctc gggctccccc tcgtgcacgc agtagggccg
    26041 gccggcgacg tcgacgaccg cgagcacgag cgcctcgtcg aggggcaccg tggcgtcacc
    26101 gaagcgggcg atcccgctct tgtccccgag ggcctcgcgc acggcctgtc cgaggacgat
    26161 cgcgacgtcc tcgacggtgt ggtgcgcatc gatgtgggtg tcgcccgtgg ccgtgacgct
    26221 gaggtcgatc agcgagtgct tggccaggga gagcagcatg tggtcgtaga agggcacgcc
    26281 ggtgtcgacc tgaccgcggc cgctgccgtc gaggtcgacg gtcacctcga cggtgctctc
    26341 ggaggtccca cgacgcaccg tggcggtacg aggggctggc gcgctgctca cggtcgggct
    26401 cctgggctga gggtcatggg cgtcctggtg gtgcggtgct gcggtgcgag gtccgacatc
    26461 gcctgcagga aggcggtggt ctcctgcggc gtgccggccg tgacacgaag atagtgggcg
    26521 atgccgacgt cgcgaacgag gacgccccgc tcgagcagcg ccgaccacgt cgtgtgggag
    26581 tcctccagac ccccgaagag cacgaagttc gcgtcgctcg gcaccgggtc gagccccatc
    26641 gcggtgagct cggccaccaa ccggtcacgt tcggccttga tcgcgcccac cgtctcgagc
    26701 atgaggtctg cgtgctcgag cgcggcgagg gccaccgcct gggtctgggt ggcgaggtgg
    26761 tacggcatcc gcaccaggcg ca
//
LOCUS       DAOSOC010000015        25709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1915_length_25709_cov_9.873158, whole genome shotgun sequence.
ACCESSION   DAOSOC010000015 DAOSOC010000000
VERSION     DAOSOC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 25709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25709
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1915_length_25709_cov_9.873158"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PL014_02365"
     CDS             <1..893
                     /locus_tag="PL014_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOT55969.1"
                     /translation="GKEPNKGVNPDEVVALGASLQAGVLKGERKDVLLIDVTPLSLGI
                     ETKGGLFTKLIERNTAIPTKRSEVFSTAEDNQPSVLIQVFQGEREIAQQNKPLGTFEL
                     SGIAPAPRGVPQIEVTFDIDANGIVHVTAKDRGTGKEQKITISGGSALSKEEIERMVK
                     DAEAHAEEDKQRREETETRNMGESLVFSTEKFLAESGDKVSAELRAPVDEALAALKEA
                     IKPETAASKDDIQAKIDDLNTKSQAMGAAMYAAAAEQGESGDVPGAESTDGGQPASDA
                     GDDDVVDAEVVEDDAPEDKK"
     gene            890..1537
                     /gene="grpE"
                     /locus_tag="PL014_02370"
     CDS             890..1537
                     /gene="grpE"
                     /locus_tag="PL014_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT55970.1"
                     /translation="MTEPHGPEQTDSSPLSGEVVDDSVNTEWVNDGDEVAPAAAGAAP
                     AAADEGEAAATASADGVDAVGTHPDSILAAERLGDLQRLQAEYVNYKRRVDRDRALMQ
                     ERAVRDVLESVLPVLDDIQAARDHGDLVDGPFAAIADKLEATLGKYGLERYGAKGEAF
                     DPMQHEALMHAPWPEGDDDLPTDAQSTTVVTVLQPGYRAGEQVLRPARVAVADPA"
     gene            1569..2618
                     /locus_tag="PL014_02375"
     CDS             1569..2618
                     /locus_tag="PL014_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOT55971.1"
                     /translation="MASQDWFEKDFYAILGVPKDADQAAIKKAYRKLARQHHPDRNAG
                     DAAAERRFKDIGEAHSVLSDKKQREEYDAVRQMAQGGARFRAGGPGGAGGFEDVFSAF
                     GGGGGGQRVRFSTGGPGGAGGAGAQPDLDDLLAQMFGSGAGAGAGARGARGGDPFSGF
                     GAPRGPRPGADVQARTTLSFRDAVQGATVTLSTPEGRRITTKIPAGVKDGQKIRLRGK
                     GADGDPGAPKGDLILTVTVEPHPVFGRDGDNLTVDLPVTFSEAALGATVAVPTLDGTP
                     VRVKVAPGTPSGRVLRVKGRGVSGRGGTGDLLAKVTVIVPQRLTDAAREAVEVLREQE
                     ADVDPRAGLFEQASS"
     gene            2630..3088
                     /locus_tag="PL014_02380"
     CDS             2630..3088
                     /locus_tag="PL014_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT55972.1"
                     /translation="MTSFQYAAFSPDDETPVFVISVAAELAGMHAQTLRQYDRLGLVT
                     PSRTRGGGRRYSQRDVALLREVQRLSQDEGVSLSGIARILELENQVAALRSRVAELTA
                     ELDAVRAASGRVTRVFSVASSGDVYAARLGERPRHGRSQALVVWNPSRSS"
     gene            complement(3189..3998)
                     /locus_tag="PL014_02385"
     CDS             complement(3189..3998)
                     /locus_tag="PL014_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOT55973.1"
                     /translation="MATSTPTASPRTAPTRSDRTRQVAVTLAEIFCVIGTLVGVGVIG
                     TRVEESSGGALAADATLIAPAGPAFSIWTPIYLGLAAYTVWQWLPDQATDRRHRAIGW
                     LAAASMVLNALWLLVTQQGWIWVSVLVMAGLVVTLGLLVARLQANPSYGHAETVIVDG
                     TFGLYLGWVAVAACANVTAAFVASGVDPGGLVAEVLAAAVLAVAALVAVILARRLGGR
                     WAVAAAIVWGLGWIAVGRLTDEPRSALVAVAAIAAAAVTVAATARQRTTAA"
     gene            4206..5678
                     /locus_tag="PL014_02390"
     CDS             4206..5678
                     /locus_tag="PL014_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT55974.1"
                     /translation="MSRAELRYPPQTYYRSVALGTVFPGLGLLRTRWRALGAGLIAVT
                     IAVLGFALVRLWRGGVLRSVLDASVRPEVLQWVGLGVLVGAVLWVGSIVLTAEQTWPR
                     RQTAGRWARVAFAAVACLIIAAPASLAVQFLEIQTSVINESFGGVTDDNNNGLTGGTK
                     QDPWAGTERVNTLLIGSDAGKDRVGVRTDSLMVVSTNTKTGDTLLIGIPRNLEFVPIP
                     DDNPLSTVWPDGYNCGDECLMNGIWTLAEDRRDLFPGVESPGRQSTIDVVGAITGLEI
                     DHSVVVNLRGFTALVDAMGGVEINVQERVCTDCSSRPGGGIKWTDGVERWIEPGVQRL
                     DGHDALWYSRSRAGKDDFSRMRRQRCMAGALIDQADPVSLLRNYPQLAAVVKNNIDFD
                     IPQQDLPAWVDLILRVQDGGSIRSLPFTNKVVSPGNPDYAEIRRLVEKSLDPPAKSTA
                     SPKPSATSSSTSTTGTEPSPSPSPTESPDDSEANELAATC"
     gene            5695..6219
                     /locus_tag="PL014_02395"
     CDS             5695..6219
                     /locus_tag="PL014_02395"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HOT55975.1"
                     /translation="MSDDGRPLHVVVMGVSGCGKSTVAARAAAALGWPFAEGDDAHPP
                     ANVAAMRSGRALTDDDRWPWLNDLAAWIAGHDAAGRSSVLACSALRRPYRDVLRSGAG
                     RVLFVHLDGPPEVIRSRLAERRGHFMPASLLASQLAAIEPLAPDENGLVLDLRHPPGE
                     LVEQLVAHVSQDKS"
     gene            6290..8842
                     /gene="clpB"
                     /locus_tag="PL014_02400"
     CDS             6290..8842
                     /gene="clpB"
                     /locus_tag="PL014_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOT55976.1"
                     /translation="MELKPTAKVAEALAIAQRTAQTAGHAEITPAHLVLALVEQEGTT
                     TPALLEAAGGSVDAAAASARSTLSGMPRSTGATVASPALGQAALGVLQQASTLMTARG
                     DTYLSTDLLVLALVEKGALRGDAKALEARIDELRGGSRVATENPEETGEALAKYGTDL
                     TEAAREGRLDPVIGRDDEIRRVVQVLSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVDG
                     DVPESLRGKRLIALDLGAMVAGAKYRGEFEERLKAVLEEIKDSAGQVVTFIDELHTVV
                     GAGATGESAMDASNMLKPMLARGELRLVGATTLDEFREHVEKDPALERRFQQVFVGEP
                     SVEDTIAILRGLKERYEAHHKVEIEDSALVAAASLSDRYITGRQLPDKAIDLVDEAAS
                     RLRMEIDSSPVEIDVLRRAVDRLRMEELHLAKETDDASRERLDRLRADLADKTEELAS
                     LTARWESEKSGLNRVGDVKARLDDLRTQAERLQRDGDYEGASRVLYGEIPALERELEA
                     AQQAESATASADLMVKERVGADDIAEVISAWTGIPAGRLLQGETEKLLAMESIIGSRL
                     IGQAEAVRAVSDAVRRSRAGVADPDRPTGSFLFLGPTGVGKTELAKSLADFLFDDERA
                     MVRIDMSEYSERHAVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLLDEVEKAHPET
                     FDILLQVLDDGRLTDGQGRTVDFRNVILVMTSNLGSQFLVDPLLEPEAKRDAVMAAVR
                     QAFKPEFLNRLDEVVLFDPLSRDELADIVHLQVRALGTRLRDRRITLEVTDAATAWLA
                     EQGFDPAYGARPLRRLVQREIGDRLATALLAGSVRDGDTVTVDRGEDGLTLR"
     gene            8994..9683
                     /locus_tag="PL014_02405"
     CDS             8994..9683
                     /locus_tag="PL014_02405"
                     /inference="COORDINATES: protein motif:HMM:NF018822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HOT55977.1"
                     /translation="MRRLFALIAAAGLVAVLAGCSGEAEAPQVPPTEQLAAAKTAFDA
                     AKTVSLTLTSRDVPPRENGVTAAKGVGVIDATEPKFQGTITGTVEGIAATIDAIAIGD
                     TAYLKLFTPQYEETDLTTLNAPNPAQFFNPATGISSLLPKTTDAKAAGQTRSGQDVLD
                     RIDGTLDGALVEDLLHLGDGTGTFTVSYGLTESNELRTATLKGPFFPGVEATYLLTMT
                     DYGAPATITRP"
     gene            9652..11322
                     /locus_tag="PL014_02410"
     CDS             9652..11322
                     /locus_tag="PL014_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT55978.1"
                     /translation="MAPQRRSPAPRALLTTAAIAVALAAADTYVVVLALTDMMRGVGV
                     GIDSLQRATPIVSGFLLGYVAVLPLVGRLADLVDRQRVLLACLGVFVIGSVVTALAVE
                     LPVLVAGRVIQGVGGGGLVPATLAIVAQLWPPDRRGVPLGVVGAVQELGSVLGPVLGA
                     LVLVIADWRAIFWLNAVLGLLLAGAVLWVGGGALPRPRPHVVLLAGLAATVGILALAA
                     PESLATSVRWGVPFVPFGDSTSRLATPIGLVAGVLILAALLVGTRGAWALLRRADLLG
                     ALLVGGALGCIVLTFAAAEPETEVVGPLGYALLPVAVLLSIAYAVHHRRSSSPLVPRG
                     LVRGRLVWALVVSVLVGVALVAVIVDVPLLARLVHTTDQTEAALVLVRFLIAVPVGAL
                     AGGWALRLLGNGVVAAAGLALAAVGLAVMTTWGRGSLEQGSSTVVLAAVGLGIGLALA
                     PVNDAALAGATHDAHGTASALVVVARMVGMVVGIALLTAVGLHQYYIAVAALPDPTDT
                     DALKDAAVLQVAWAFRGAAVAAALGALASLALGVRRRAGEERSAILGL"
     gene            11408..>12944
                     /locus_tag="PL014_02415"
     CDS             11408..>12944
                     /locus_tag="PL014_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HOT55979.1"
                     /translation="MDIGGCGLGERRSAIETAREALRGLGAVLHEAPGSELAELMGVV
                     DDVAAQAGAARVAITVEAMGRGEIEAAGVNVHTWVRDHAPSLRQGGAFQVARVACAVS
                     PTPSRWCPQGQVVDPGSPLGIVWAEVQDGTVSPALACAALREVERLAPIVRDEVLPTV
                     TASMLELGVAWGPTQLRKLRPRLIAEFGRAGKFDDLQARLASAARLSMPVVESGDLTE
                     YQLVMTPEQAAALEAAIGPLSAPAPNDETGERDLRPAGQRRVEALALVCQRSSAADAD
                     QRGGVDGAAGSGAALHVSMTVTDLQAGTGFGEVLASTATGAMLSPSVVRRLACSAAVV
                     PQVLGSAGEPLDEGRVERLFTRAQRRVLMGRDRGCTYPGCGAPAAWTQAHHVVHWLDG
                     GATDLDNAALLCQRHHTVVHDRRYVAQVRATPDELGRFVVWDRTVGSYDQYLEAVRAE
                     RAVADPRPLTPQRLAELLAAIRADDPDEQHWARLELDLERDEHEQWRQSQDWADLLES
                     MPPR"
     assembly_gap    12945..13044
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13045..13568)
                     /locus_tag="PL014_02420"
     CDS             complement(<13045..13568)
                     /locus_tag="PL014_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOT55980.1"
                     /translation="MRIATWNVNSIRSRIDRVEAWLARSQVDVLAIQESKAKDEQFPY
                     DRFRALGYEVAHHGVSQWNGVGIASRVGLDDVQVGFDGAPGWGEPATAEARAIGATCG
                     GIRMWSLYVPNGRALDDPHMAYKLSWLDRLREQATGWAADGTPVALCGDWNIAPTDDD
                     VWSMEYYADKSHVSP"
     gene            complement(13611..13916)
                     /locus_tag="PL014_02425"
     CDS             complement(13611..13916)
                     /locus_tag="PL014_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOT55981.1"
                     /translation="MGLLKTAAKVAVASRVHGNVQRRQSQRWAAQDAAAAASAPAHAP
                     VHAAAPSPVAAPPAPPALSQASGTEEMLQQLERLGQLRAAGVLTDAEFETQKARILA"
     gene            complement(13948..14355)
                     /locus_tag="PL014_02430"
     CDS             complement(13948..14355)
                     /locus_tag="PL014_02430"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HOT55982.1"
                     /translation="MSYVAVEFPDGRADAAGFAALRDLVDRGLVIDLDLEFIRRGEDG
                     ALVTVPAASLDLGADLSAFAGTDAGLLDRDDLELVAGSLGGDAVMAVLVYEDLTLQPV
                     LGAWAASGARLVAEGAVDPVELDLALGDDTLTP"
     gene            14500..15273
                     /locus_tag="PL014_02435"
     CDS             14500..15273
                     /locus_tag="PL014_02435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT55983.1"
                     /translation="MTTALVTGASAGIGHAFAQRLAREGHDLVVVARDRARLERLAAE
                     LRAAHGVDVEVLVADLSDRHATDAVCARLADAERPVDLLVNNAGFGLAGSFLDNDVAQ
                     EEAGLDVMVRAVMLTCHAAGRAMRARGHGAIVNVSSVASFIAHGTYSAEKAFVTVFSE
                     GLASELAGTGVTVTALCPGFTRTEFHQRARMKFSALPEFLWLDADDLVQQALTDVAAG
                     KVISVPGAQWKVLATTVRALPRPLVRGAGVRALHRFRRD"
     gene            15305..15859
                     /gene="pyrE"
                     /locus_tag="PL014_02440"
     CDS             15305..15859
                     /gene="pyrE"
                     /locus_tag="PL014_02440"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT55984.1"
                     /translation="MTDIAADRARLLQIVRDKAIVHGRVTLSSGREADYYVDLRRITL
                     DGEASPLVGRVMLDLTADLDFDAVGGLTLGADPVATAMLHARAAQGGRLDAFVVRKAG
                     KAHGLQQRIEGPSIEGRRVLIVEDTSTTGSSPLDAAQAARDAGATVVAVATIADRATG
                     AAEKFADAGLEYRHVFGLADLGLA"
     gene            complement(15911..17518)
                     /locus_tag="PL014_02445"
     CDS             complement(15911..17518)
                     /locus_tag="PL014_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HOT55985.1"
                     /translation="MGENSMRDSKTPQMGSATKTLGIIGAVVAVFYAIIIALSPKPIT
                     FPSPVPEFAPDATLFDKVSGWITGLVFYGPSANGEYTIRIVVIWLITAAIFFTIWLGF
                     INVRGFKHAIDLVRGKYSDPNDAGEVSHFQALATAVSGTVGLGNIAGVAIAIAIGGPG
                     ATFWMIVAGFLGMSLKAAECMLGVKYRQEHADGTVSGGPMYYLRDAVKDLGKPGLGKG
                     LAAFFAVCMIGGAVGGGNMFQSNQATAQIVSQVGGEESFLGRNTWIMGLLFAAAVGAV
                     IIGGIKSIAKVTEKIVPFMAVLYLGACLIVLLGNFGEIPAAFGSILDGAFTGEGAAGG
                     VIGALIVGFQRAAFSNEAGLGSASVAHSAVRTKEPATEGFVALLEPFIDTIVICTMTA
                     LTIIITKAYLDPAASELGGVALTSNAFASVLPWFPKVLAVAVVLFAFSTMISWSYYGM
                     KATGYLFGDNPLAENVFKVIFLGFTVLGSVASLGSVIDFSDSMIFMMSIPNLIGLYLL
                     RNVIRREFSHYRARLASGEIKEFADRH"
     gene            complement(17518..17949)
                     /locus_tag="PL014_02450"
     CDS             complement(17518..17949)
                     /locus_tag="PL014_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT55986.1"
                     /translation="MDTIVVGVDNSEASTRAVEFAVDRAQKNHWKIVLVHVIPWTPFS
                     FQTASENEHRHRERQRELEAATEQVIAPMVEICERGGVPHEEVVRHGKASETLLDISA
                     ESGAVHIIVGRTGDSGLRDAVFGSVANRLAQHASVPVTVVP"
     gene            18070..18726
                     /locus_tag="PL014_02455"
     CDS             18070..18726
                     /locus_tag="PL014_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOT55987.1"
                     /translation="MTAALVIVIAIVVLLIAVVGWAIATYNGLIKLRNLVQEAWRQID
                     VELTRRHDLIGNLVETVKGYAAHERGTLEDVIKARAGAMAPGQSPAQQAESENMLSQA
                     LGRLLAIAEAYPDLKANQNFMALQAELTSTEDRIASARRYYNATVRDLNTKVETVPSN
                     IIAGVFNIARAEYFEAVGEQREAPKVDFGQREAVIPPPSGYAGGSGNGPQDTPPAPQA
                     "
     gene            18817..19689
                     /locus_tag="PL014_02460"
     CDS             18817..19689
                     /locus_tag="PL014_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55988.1"
                     /translation="MDDAITIRGLRKAYGGRAAVDGLDLDIRTGEVFALLGPNGAGKT
                     TTVEIAEGFRSRDAGEVRVLGTDPEGAGRSWRNQIGIVTQTSAGLDLLTPREALASTA
                     KVYSGPRDVDEVIAAVGLADKADTRIVGLSGGQRRRLDVGLGIVGGPELIFLDEPTTG
                     FDPQARRDFWTLIRALADQGTTILLTTHYLDEAEELADRVGVIAAGRLLALDTPAALG
                     GRGTSEATVTWEEDGIRRSLRTATPTAQVSRLAARMPGGEVPGLTVTRPSLEDTYLTL
                     IAPHITDDTTEVAS"
     gene            19686..20489
                     /locus_tag="PL014_02465"
     CDS             19686..20489
                     /locus_tag="PL014_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55989.1"
                     /translation="MNALALGLDRTILELKMYSREKESVFFSFLFPILLLSLFSVIFS
                     AQFQEGAGQGGMTAGLFFMPGMLSAGVLLTSFQTMALSIATERDDGTLKRLRATPMPS
                     VAYFLGKVGLVGVTSVLQTVLLLVVATLFFDVDLPTDPERWAVFVLVFGLGVFSGTVL
                     GIAYSSLATSRSIGAVVIGPMLVLQFISGVYIAFDNVPTWLQQVASLFPLKWIAQGMR
                     SVFFPAEMAAGEMAGSWETGKTALVLLAWGIAGLVLCITTFRWFKRGTT"
     assembly_gap    20690..20789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20790..21457
                     /locus_tag="PL014_02470"
     CDS             <20790..21457
                     /locus_tag="PL014_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT55990.1"
                     /translation="TIDELRAAQAELAASERDRGVHEERERLSREIHDTLAQGFTSVV
                     ALSRAADAALARGDVDAARDRLALIERTASDNLDEARLIVAELTPGHLQSRTLGEALH
                     RLGAAVSSENGLRADVRVTGEPVPLGGTAEVVILRTAQEALSNVRRHAGAGHAELILT
                     YEHPEQVVLEVRDDGRGFDPAAARSGFGLDGVQARAAEVGGSVRLRSEPGAGTTLRLE
                     VPR"
     gene            21454..22086
                     /locus_tag="PL014_02475"
     CDS             21454..22086
                     /locus_tag="PL014_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT55991.1"
                     /translation="MIRLLVVDDHPVVRAGMVAVLSEEADFEVVGEAANGAEALALVP
                     RLMPDVVLMDLRMPVMDGADATSRIVAQSDAPQVLVLTTYDTDADIVRAVEAGARGYL
                     LKDAPTAVLADAIRRAARGETVLAPPVAARLADRLRAPSRPELTARELDVLRLVAIGL
                     SNGDIGRELFIGEATVKTHLIRVFAKLEVTDRTAAVTAAYAQGLIDLPRP"
     gene            complement(22090..22764)
                     /locus_tag="PL014_02480"
     CDS             complement(22090..22764)
                     /locus_tag="PL014_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOT55992.1"
                     /translation="MPSLGPDWMDPQWLLDRFGEQFFWVSVAIVFIECGLLFPILPGD
                     SLLFAVGLFIARGDIDLNIVVACAILSAAAFAGNVTGYEIGRAIGAPLYERDGRFIKK
                     KYFDQTHDFFEKYGAKALVIGRFVPIVRTFITVVAGVSRMNRRHFFVWSAVGAVLWAT
                     GITLLGYFLGGIDFVHDNLEAAVLLIVAISVLPMVFEFLRHRSATKTVIDEIDEVVDD
                     VRDGTL"
     gene            22906..23796
                     /locus_tag="PL014_02485"
     CDS             22906..23796
                     /locus_tag="PL014_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT55993.1"
                     /translation="MPTTRATRATRLFGVALTGSALLALAACGSPTDEPAASASGDAT
                     ASAATCTPETMKTLTAGTLTIGTDKPAYQPWFVDDDPSNGKGYESAVAYAIADELGFA
                     KDKVTWVVAPFNTVVAPGKKAFDFDVNQVSISDERKQAVDFSSGYYDVRQAVITYKGS
                     PIDGKTTVADLKGAKLGAQVGTTSYNAAKDQVQPSAAVAVFDTNDLAVQALKNKQIDG
                     IVADLPTAFYMTAAQLDDGVIVGQLPLQGEPEQFGAVLDKGSPLTGCVTQAVDTLREN
                     GTLDTLVNTWLSTQGAPELK"
     gene            23827..24708
                     /locus_tag="PL014_02490"
     CDS             23827..24708
                     /locus_tag="PL014_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOT55994.1"
                     /translation="MGPTESTGEPVEDRWVPSALEQERAAYRSARTRRSAVVATASTL
                     AMLGAIVALVVSSPGWPRVKETFFSWDKAVDSLPAVLDGLWLNIRVMLVCAVLIAILG
                     LTLAVMRTVRGPVAFPLRLFATAYVDLFRGLPLLLVLFLLGFGAPALQLSGVPNSALF
                     WGGCALVLSYSAYVAEVFRAGIESVHPSQRAAARSLGLSYGQTLRHVVLPQAVRRVVP
                     ALMNDLVSLQKDSGLIAVLGVIDAIRAAQIETASDFNYTPYVVAGALFVCLTIPMARF
                     TDWVARRQGFHGAGGHL"
     gene            24731..25462
                     /locus_tag="PL014_02495"
     CDS             24731..25462
                     /locus_tag="PL014_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOT55995.1"
                     /translation="MSLLALEGVRKSFGKNVVLQDIDLTVERGQCVVLIGASGSGKST
                     LLRCVNLLEVVDDGRITLDGDDITDPRIDADAVRARMGVVFQAYNLFPHLSVVDNITL
                     APIRVHGVPRGEARDKAMAMLDRVGLRDRAGARPDDLSGGQQQRVAIARALVNDPTLM
                     LLDEVTSALDPELVGEVLDLLRSLTQEGMTMILNTHEMGFARQVADTVCFLDGGVIHE
                     QGPPEQVLGDPREERTRQFLSRVHA"
     gene            25479..>25709
                     /locus_tag="PL014_02500"
     CDS             25479..>25709
                     /locus_tag="PL014_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HOT55996.1"
                     /translation="MSGHDEPADVGVGPWQGPWPDGDHFDPDLLREGDRRNVADRYRY
                     WTMDAVVADLDTRRHPFHVAIENWGHDFNIGSV"
BASE COUNT         3736 a         8942 c         9115 g         3716 t
ORIGIN      
        1 gcggcaagga gcccaacaag ggcgtcaacc ccgacgaggt cgtggccctg ggcgcgtccc
       61 tgcaggccgg tgtcctcaag ggtgagcgca aggacgtcct gctcatcgac gtcacgccgc
      121 tctcgctcgg catcgagacc aagggtggcc tgttcaccaa gctcatcgag cgcaacacgg
      181 ccatcccgac caagcgcagc gaggtcttct ccacggccga ggacaaccag ccctcggtgc
      241 tcatccaggt cttccagggt gagcgtgaga tcgcgcagca gaacaagccg ctcggcacct
      301 tcgagctctc cggcatcgcc ccggcccccc gtggcgtccc gcagatcgag gtcaccttcg
      361 acatcgacgc caacggcatc gtccacgtga ccgccaagga ccgcggcacc ggcaaggagc
      421 agaagatcac catctcgggc ggcagcgcgc tgtccaagga ggagatcgag cgcatggtca
      481 aggacgccga ggcccacgcc gaggaggaca agcagcgccg cgaggagact gagacccgca
      541 acatgggcga gtcgctcgtg ttctcgaccg agaagttcct cgccgagtcc ggcgacaagg
      601 tctccgccga gctgcgtgcc ccggtcgacg aggccctggc cgccctcaag gaggccatca
      661 agccggagac ggcggcgtcc aaggacgaca tccaggccaa gatcgacgac ctgaacacca
      721 agtcccaggc catgggtgcg gcgatgtacg cggccgcggc cgagcagggc gagtccggcg
      781 acgtgccggg tgccgagtcc accgacggtg gccagccggc atccgacgcg ggtgacgacg
      841 acgtcgtcga cgccgaggtc gtcgaggacg acgcgcccga ggacaagaag tgaccgagcc
      901 ccacggcccg gagcagaccg acagcagccc gctcagcggt gaggtcgtcg acgacagcgt
      961 caacaccgag tgggtcaacg acggcgacga ggtggccccg gcagcagccg gggccgcccc
     1021 cgccgccgcc gacgagggcg aggcggctgc cacggcatcc gccgacggtg tggatgccgt
     1081 gggcacccac ccggattcga tcctcgccgc cgagcggctc ggcgacctgc agcggctcca
     1141 ggccgagtac gtcaactaca agcgcagggt cgaccgcgac cgggcgctga tgcaggagcg
     1201 cgcggtgcgt gacgtcctcg agtcggtgct gccggtgctc gacgacatcc aggccgcgcg
     1261 ggaccacggc gacctcgtcg acggaccgtt cgcggccatc gccgacaagc tcgaggcgac
     1321 gctcggcaag tacggtctcg agcgctacgg ggccaagggc gaggccttcg acccgatgca
     1381 gcacgaggcc ctgatgcacg cgccgtggcc cgagggcgac gacgacctgc cgaccgacgc
     1441 gcagtccacg acggtcgtca ccgtgctgca gccgggctac cgtgcggggg agcaggtcct
     1501 gcgccccgcc cgggtcgcgg tcgcagaccc cgcctgatcc actgacgagg caccgagagg
     1561 aggcgcccat ggccagccag gactggttcg agaaggactt ctacgcgatc ctcggcgtcc
     1621 ccaaggatgc cgaccaggcc gcgatcaaga aggcgtaccg caagctcgcg cggcagcacc
     1681 acccggaccg caatgccggg gacgctgccg ccgagcggcg gttcaaggac atcggtgagg
     1741 cccactccgt gctctccgac aagaagcagc gcgaggagta cgacgcggtg cgccagatgg
     1801 cgcagggcgg tgcccgcttc cgcgccggtg gccccggtgg ggccggcggc ttcgaggacg
     1861 tcttctcggc cttcggtggc ggagggggtg gccagcgggt gcgcttctcc acgggcggcc
     1921 cgggcggcgc cggtggtgcg ggggcccagc ccgacctcga cgacctcctc gcgcagatgt
     1981 tcggcagtgg cgccggggcg ggcgccggtg cgcgtggagc ccgcggcggc gacccgttct
     2041 ccgggttcgg agccccccgt ggacctcgcc cgggcgctga cgtccaggcc cgcacgaccc
     2101 tgagcttccg cgatgccgtt cagggcgcca cggtgaccct gtcgaccccc gagggtcgcc
     2161 ggatcacgac caagattcct gcgggggtca aggacgggca gaagatccgg ctgcggggca
     2221 agggagccga cggtgacccc ggggcaccca agggcgacct catcctcacg gtgaccgtcg
     2281 aaccgcatcc ggtcttcggg cgcgacggtg acaacctcac cgtcgacctg ccggtgacct
     2341 tctccgaggc cgcactcggg gccaccgtcg ccgtgcccac cctcgatggc accccggtac
     2401 gggtcaaggt ggcaccgggg accccgagcg gccgggtgct gcgcgtcaag ggacgtgggg
     2461 tctccgggcg aggcggcacc ggtgacctgc tcgccaaggt gaccgtcatc gtgccccagc
     2521 gactcaccga cgccgcccgc gaggcggtcg aggtcctgcg ggagcaggag gccgacgtcg
     2581 acccacgcgc cggcctgttc gagcaggcga gcagctgaag gaggacgcca tgacgtcgtt
     2641 ccagtacgcg gccttcagcc ccgacgacga gaccccggtc ttcgtcatct ccgtcgcggc
     2701 cgagctcgcc ggcatgcacg cccagaccct gcgtcagtac gaccgactgg gcctggtcac
     2761 cccgtcgcgc acccgcgggg gcggtcgccg gtactcccaa cgtgatgtcg ccctgctgcg
     2821 cgaggtgcag cggctctccc aggacgaagg tgtgtccctg tcgggcatcg cccgcatcct
     2881 cgagctcgag aaccaggtgg ccgccctgcg ctcgcgggtg gccgagctga ccgccgagct
     2941 ggatgccgtt cgggccgcat ccggtcgcgt cacccgggtc ttctcggtcg cctcgagcgg
     3001 cgacgtgtac gccgcgcgcc tcggcgagcg gccacggcac gggcgctcgc aggcgctcgt
     3061 cgtgtggaac ccgagccgct cctcctgacc cgggcttcct gacttttcct ccccatgagg
     3121 gttacgcgag gttcgcaagg gaaggcatcc cttgtgaacc tccgtcttcc cttgtgaagg
     3181 gccgaggctc aggcagcggt ggtgcgctgc cgggccgttg cggccacggt gacggccgct
     3241 gcggcgatcg cggcgacggc gaccagcgcg gaccggggct cgtcggtgag gcgcccgacg
     3301 gcgatccacc ccaggcccca gacgatggcg gccgcgaccg cccaccggcc cccgagccgg
     3361 cgcgcgagga tcaccgcgac gagggcggcc accgcgagca ctgcggcagc aagcacctcg
     3421 gcgaccaggc caccggggtc gaccccgctc gccacgaacg cggccgtgac gttggcgcag
     3481 gccgccacgg caacccagcc gaggtagagc ccgaaggtgc cgtccacgat gaccgtctcg
     3541 gcatggccgt acgacgggtt cgcctgcagc cgggccacga gcaggccgag ggtcacgacg
     3601 agcccggcca tgacgagcac gctcacccag atccagccct gctgggtgac gagcagccag
     3661 agggcgttga gcaccatgga tgccgctgcg agccatccga tcgcacggtg ccggcggtcg
     3721 gtcgcctggt cgggcagcca ctgccacacg gtgtaggcgg ccaggccgag gtagatgggg
     3781 gtccagatcg agaaggcggg gccggcgggg gcgatgagcg tggcgtccgc ggccagtgcg
     3841 cccccggacg actcctcgac ccgggtgccg atgacaccga cgccgacgag cgtcccgatg
     3901 acgcagaaga tctcggcgag ggtgacggcg acctgccgag ttcggtccga gcgggtgggg
     3961 gcggtccggg gggaggcagt cggggtcgag gtggccatgc cgtcattgca ccacgggccc
     4021 ggagggacac gggtcccacg agcagcggca ttcagggcag tttcaggttg gggcgatatc
     4081 gtagatgcgt tgcgcacgtc gtgcctcggc tgttgtgcgt cttcacgaga cgagaaggtc
     4141 gatagggcat gtccgacgaa gagccgttga gctctccagg gcggccggga ggctcccggc
     4201 cgccggtgag tcgtgccgaa ttgcgctacc cgccgcagac ctactaccgc agcgtcgccc
     4261 tcggcacggt gtttcccggc ctgggcctgc tgcgcacccg gtggcgcgct ctcggagccg
     4321 gcctcatcgc cgtgacgatc gccgtgctcg gcttcgccct cgtgcgcctc tggcgcggag
     4381 gggtcctgcg ttcggtgctc gacgccagtg tccgccccga ggtgctccag tgggtcggcc
     4441 tcggcgtgct cgtcggcgcg gtgctctggg tcgggtcgat cgtcctcacg gcggaacaga
     4501 cctggccgcg ccggcagacg gccggtcgtt gggcgcgagt ggccttcgcg gcggtggcgt
     4561 gcctcatcat cgcggcaccg gcctcgctcg ccgtgcagtt cctcgagatc cagacctccg
     4621 tcatcaacga gtccttcggc ggcgtgaccg acgacaacaa caacggcctc accggtggca
     4681 ccaagcagga tccctgggcg gggaccgagc gcgtcaacac gctgctcatc gggtcggatg
     4741 ccggtaagga ccgcgtcggg gtgcgcaccg actcgctcat ggtggtcagc acgaacacca
     4801 agaccggtga cacgctgctc atcggcatcc cgcgcaacct cgagttcgtt cccatccccg
     4861 acgacaaccc gctgtcgacg gtctggccgg acggctacaa ctgtggcgac gagtgcctga
     4921 tgaacggcat ctggaccctg gccgaggacc gtcgggacct cttccccggg gtcgagagcc
     4981 ccggccggca gtcgacgatc gacgtcgtag gagccatcac cggcctggag atcgaccact
     5041 ccgtcgtcgt caacctgcgg gggttcaccg ccttggtgga cgccatgggt ggtgtcgaga
     5101 tcaacgtcca ggagcgggtg tgcaccgact gctccagccg acccggtggc ggcatcaagt
     5161 ggaccgacgg ggtggagcgg tggatcgagc ccggcgtcca gcgcctcgac ggccacgacg
     5221 ccctgtggta ctcgcgctcg cgcgccggca aggacgactt cagccggatg cgccgtcagc
     5281 gctgcatggc cggcgccctc atcgaccagg cggacccggt ctcactcctg cgcaactacc
     5341 cccagctggc cgcggtcgtg aagaacaaca tcgacttcga catcccgcag caggacctgc
     5401 cggcgtgggt cgacctcatc ctgcgcgtgc aggatggcgg gtccatccgc tcgctgccgt
     5461 tcaccaacaa ggtcgtcagt cccggcaacc ctgactacgc cgagatccgc cggctcgtgg
     5521 agaagtcgct cgacccgccc gcgaagtcca cagcatcccc caagccgtcg gccacgtcga
     5581 gctccacatc gaccacgggc accgagccgt caccgtcccc gtcgccgacc gagtcccccg
     5641 acgactcgga agccaacgag ctcgcggcga cctgctgaac ggcatccgcc tcccgtgagc
     5701 gacgacggcc gacctctcca cgtcgtggtc atgggggtgt cgggctgcgg caagagcacc
     5761 gtcgccgcga gggctgccgc cgctctcggg tggccgttcg ccgaggggga cgacgcccac
     5821 ccgccggcga acgtggccgc catgcggtcg gggcgcgccc tcaccgacga cgatcgatgg
     5881 ccgtggctga acgacctggc ggcgtggatc gccgggcacg acgcggccgg ccggtcgagc
     5941 gtcctggcgt gctcggccct gcgccggccc taccgtgacg tgctgcgttc gggggccggc
     6001 cgggtgctgt tcgtgcacct cgacggcccg cccgaggtga tcaggtcgcg gctggccgaa
     6061 cgcaggggcc acttcatgcc cgcatcgctc ctcgcctctc agctggcggc aatcgagccg
     6121 ctcgcgccgg acgagaacgg tctggtcctg gacctgcgac accctccggg cgagctcgtg
     6181 gagcagctgg tggcccacgt gagccaggac aagagttgag tggaatagac tcaactttgg
     6241 aacggttgtc cctgatgtag atctgccctg gttgctgcaa ggagacccca tggaactgaa
     6301 acccaccgcg aaggtggccg aggcgctggc cattgcccag cgcacggccc agaccgcggg
     6361 tcacgccgag atcaccccgg cacacctcgt gctcgccctg gtcgagcagg agggcacgac
     6421 caccccggcg ctgctggagg cggccggagg ttcggtcgac gccgctgcgg cgtcggcccg
     6481 ttcgacgttg tcggggatgc cgcgcagcac cggagcgacc gtcgcctcgc cggcgttggg
     6541 tcaggcagcg ctcggtgtgc tccagcaggc gtcgacgctg atgaccgcac ggggggacac
     6601 ctacctctcc accgacctgc tcgtgctggc cctggtcgag aagggcgcgc tgcgcggcga
     6661 cgccaaggca ctcgaggccc ggatcgacga gctgcgcggg ggctcccggg tggccaccga
     6721 gaacccggag gagaccggcg aggcgttggc gaagtacggc accgacctca ccgaggccgc
     6781 ccgtgaggga cggctcgatc cggtcatcgg tcgagacgac gagatccgtc gcgtcgtcca
     6841 ggtgctcagc cgccgcacga agaacaaccc cgtcctcatc ggcgaaccgg gtgtcggcaa
     6901 gaccgccgtc gtcgagggtc tggcccagcg gatcgtcgac ggcgacgtgc ccgaatcact
     6961 gcggggcaag cgcctcatcg ccctggacct cggggccatg gtcgccgggg ccaagtaccg
     7021 cggcgagttc gaggagaggc tcaaggccgt gctcgaggag atcaaggact ccgccgggca
     7081 ggtcgtgacc ttcatcgacg agctgcacac cgtcgtcggc gccggggcga ccggggagtc
     7141 cgcgatggat gcgagcaaca tgctcaagcc catgctcgcc cgtggggagc tgcgcctcgt
     7201 cggggcgacg accctggacg agttccgcga gcacgtcgag aaggaccctg cgctggagcg
     7261 ccgcttccag caggtcttcg tcggcgagcc gagcgtcgag gacaccatcg ccatcctgcg
     7321 cggcctcaag gagcgctacg aggcgcacca caaggtcgag atcgaggaca gtgccctggt
     7381 tgccgcggcc tcgctcagcg accggtacat caccggccgc cagctgcccg acaaggccat
     7441 cgacctcgtc gacgaggcag cctcgcgcct gcggatggag atcgactcca gccccgtcga
     7501 gatcgacgtg ctgcgacgcg ccgtggaccg gctgcgcatg gaggagctgc acctggccaa
     7561 ggagaccgac gacgcgtcgc gcgagcggct cgaccgcctg cgcgccgacc tcgccgacaa
     7621 gaccgaggag ctcgcgtcgc tcacggcccg ttgggagtcc gagaagtccg gcctcaaccg
     7681 ggtcggtgac gtcaaggccc gcctcgacga cctgcgcacc caggccgagc ggctccagcg
     7741 tgacggtgac tacgagggag cctcgcgggt tctctacggg gagatccctg cgctcgaacg
     7801 tgagctcgag gcggcccaac aggccgagtc ggccacggca tccgctgacc tgatggtcaa
     7861 ggaacgggtg ggggccgacg acatcgccga ggtcatctcg gcatggaccg gcatccccgc
     7921 ggggcgcctg ctccagggcg agaccgagaa gctgctggcc atggagtcga tcatcgggtc
     7981 ccggctgatc gggcaggccg aggcggtgcg cgccgtctcg gatgccgtgc ggcgctcgcg
     8041 ggccggcgtc gccgacccgg atcggccgac gggcagcttc ctgttcctcg ggccgaccgg
     8101 tgtcggcaag accgagctgg ccaagtcgct cgccgacttc ctgttcgacg acgagcgcgc
     8161 gatggtgcgc atcgacatgt cggagtacag cgaacggcac gcggtggcgc gactcatcgg
     8221 tgcccctccc ggctacgtcg ggtacgagga gggtggccag ctcaccgagg ccgtgcgacg
     8281 tcggccctac tcggtcgtgc tgctcgacga ggtcgagaaa gctcaccccg agaccttcga
     8341 catcctgctc caggtgctcg acgacggccg gctcaccgac ggccaggggc gcacggtcga
     8401 cttccgcaac gtcatcctcg tcatgacctc gaacctcggc tcgcagttcc tcgtcgaccc
     8461 gctgctcgag cccgaggcca agcgggatgc cgtgatggcg gcggttcggc aggccttcaa
     8521 gcccgagttc ctcaaccggc tcgacgaggt cgtgctcttc gacccactct cccgggacga
     8581 gctcgccgac atcgtgcacc ttcaggtgcg ggccctcggg acccggctgc gtgaccgccg
     8641 gatcaccctc gaggtcaccg acgcggccac ggcctggctc gccgagcagg gcttcgaccc
     8701 ggcctacggt gctcgcccgc tgcgccggct ggttcagcgc gagatcggcg accggctcgc
     8761 gacggcgctg ctcgccggga gcgtgcgcga cggcgacacc gtgaccgtcg accgcggcga
     8821 ggacggcctg accctgcgct gagccgggtg gcctcgccgg ctgaacttct ccgccatccc
     8881 tgtgatggcg gagaagttcg tgcatggcgc ggatatgtct ccgtcacgca cgaacgtctc
     8941 cgccacggct gtgatgaccc gtccgggggg agcaggcgga tactgttgcg gccatgcgcc
     9001 gactcttcgc cctgatcgcc gctgccggtc tcgtcgccgt gctggccggg tgctcgggag
     9061 aggccgaagc cccgcaggtt ccgcccaccg agcagctggc ggcagccaag accgccttcg
     9121 acgcggcgaa gaccgtcagc ctcaccctga ccagccggga cgtcccgccc cgcgagaacg
     9181 gcgtcaccgc cgccaagggg gtgggagtca tcgatgccac cgagccgaag ttccagggca
     9241 ccatcaccgg cacggtggag ggcatcgcgg cgaccatcga cgcgatcgcc atcggcgaca
     9301 cggcctacct caagctgttc accccgcagt acgaggagac cgacctcacg acgttgaacg
     9361 cccccaaccc ggcgcagttc ttcaacccgg ccaccggcat ctccagcctg ctgccgaaga
     9421 ccaccgacgc caaggccgcc ggccagaccc gctcgggcca ggacgtgctc gaccgcatcg
     9481 acggcaccct cgacggcgcg ctcgtcgagg acctgctcca cctcggcgac ggcaccggca
     9541 ccttcaccgt cagctacggc ctcaccgaga gcaacgagct gcgcaccgcc acgctcaagg
     9601 gaccgttctt ccccggggtc gaggccacct acctgctgac catgacggac tatggcgccc
     9661 cagcgacgat cacccgcccc tagggcactg ctcacgacgg ctgccatcgc cgtcgccctc
     9721 gccgccgccg acacctatgt cgtcgtcctc gccctgaccg acatgatgcg cggtgtcggg
     9781 gtgggcatcg actccctcca gcgggcgacg ccgatcgtct cgggcttcct gctcggctac
     9841 gtcgcggtgc tgcccctcgt cggtcggctg gccgacctcg tcgaccgcca gcgggtgctg
     9901 ctcgcctgcc tgggggtctt cgtcatcggg tcggtggtca ccgccctggc cgtcgagctg
     9961 cccgtgctcg tggccggccg ggtgatccag ggcgtcggcg gaggaggcct ggtgccggcc
    10021 accctggcca tcgtcgccca gctctggccg ccggaccggc gcggggtacc cctgggtgtc
    10081 gtcggggcgg tgcaggaact cgggtccgtg ctcggcccgg tgctcggcgc cctcgtcctc
    10141 gtcatcgctg actggcgggc catcttctgg ctgaacgcgg tgctgggtct gctgctcgcc
    10201 ggtgcggtgc tgtgggtcgg gggcggagcg ctgccgaggc cgcgcccaca cgtcgtgctg
    10261 ctcgcggggc tggccgcgac cgtcggcatc ctcgccctcg cagcccccga gtccctcgcg
    10321 acgagcgtgc gctggggagt gccgttcgtc cccttcggcg actcgacgtc gcggctcgcc
    10381 acccccatcg ggctcgtggc gggggtgctg atcctggccg ccctgctggt cggcacgcgt
    10441 ggtgcgtggg ccctgctgcg ccgcgccgac ctgctcggtg cgctccttgt cggtggggca
    10501 ctcggctgca tcgtgctgac cttcgccgcg gccgagccgg agaccgaggt cgtcggtccg
    10561 ctcggctacg cgttgcttcc cgtggcggtc ctgttgtcga tcgcgtatgc cgtgcaccac
    10621 cgccgctcgt cctcacccct ggtgccccgt ggtctggtgc gtggtcgcct cgtgtgggcc
    10681 ctggtcgtgt cggtgctcgt cggggttgcc ttggtggccg tcatcgtcga cgtcccgctg
    10741 ctcgcccgcc tcgtgcacac caccgaccag accgaggcgg cgctggtgct cgtccgcttc
    10801 ctcatcgcgg tcccggtcgg ggcgctggcc ggaggctggg cgctgcgcct gctgggcaac
    10861 ggtgtggtgg ccgcagccgg cctcgccctc gctgccgtcg gcctcgccgt gatgacgacg
    10921 tggggtcgcg ggtcgctcga gcagggctcg tcgaccgtgg tgctcgctgc agtgggcctc
    10981 gggatcgggt tggccctggc cccggtgaac gacgcggcac tcgcgggagc cacccacgat
    11041 gcccacggca cggcatccgc gctcgtcgtc gtggcccgga tggtcggcat ggtcgtcggg
    11101 atcgcgctgc tgaccgccgt cggcctgcac cagtactaca tcgcggtggc cgccctgccc
    11161 gacccgacgg acaccgacgc gctcaaggat gccgcggtgc tccaggtggc gtgggcgttc
    11221 cgtggggctg cggtggccgc tgctctcggg gcgctcgcct ccctggcgct gggggtgcgc
    11281 cgccgtgcag gtgaggaacg gtcggcgatc ctggggctgt gacgtcgtcg ctgtgggcag
    11341 gcgaatcagg tcgagtgagt tgccctcagg ggttgatcat cgaactggtg ttcgtataag
    11401 atgagtcatg gacatcgggg gatgtggtct gggggagcgg cgcagcgcga ttgagaccgc
    11461 gcgtgaggcg ttgcgtgggc tcggtgcggt cctgcacgag gctccggggt cggagctggc
    11521 cgagttgatg ggcgttgtcg acgacgttgc tgcgcaggcg ggggcggcgc gggtcgcgat
    11581 cacggtggag gcgatgggcc gtggtgagat cgaggccgcg ggagtgaacg tgcacacctg
    11641 ggtgcgtgac cacgcgccgt cgctgcggca gggcggtgcg ttccaggtgg ccagggtggc
    11701 gtgcgcggtg tctccgaccc cgtcccggtg gtgcccgcag ggacaggtcg tcgatccggg
    11761 gtcgccgttg ggcatcgtgt gggcggaggt gcaggacggc acggtgtcac cggcgttggc
    11821 gtgtgcagcg ttgcgtgagg tggagcggtt ggcgccgatc gtgcgcgatg aggtgttgcc
    11881 gacggtgacg gcgtcgatgc tggagctggg ggtggcgtgg gggccgacgc agctgcgcaa
    11941 gctgcggccg cggctgatcg cggagttcgg gcgggcgggg aagttcgatg atctgcaggc
    12001 ccggttggcg tcggcggcgc ggttgtcgat gccggtggtg gagtccggtg atctcaccga
    12061 gtaccaactg gtcatgacgc cggagcaggc ggcggcactc gaggccgcga tcgggccgtt
    12121 gtccgcgccg gcgcccaatg acgagacggg ggagcgagac cttcgcccgg cggggcagcg
    12181 gcgggtcgag gcactggcgt tggtgtgcca gcggtcgtcc gccgcagacg cggaccagcg
    12241 cggtggtgtc gacggggctg ctgggtcggg tgcggcgctg cacgtgagca tgacggtgac
    12301 agaccttcag gcgggcaccg ggtttggtga ggtgctggcc tcgaccgcga cgggggccat
    12361 gctctccccg tcggtggtgc ggcgtctggc gtgctcggcg gcggtcgtgc cgcaggtgct
    12421 ggggtcagcc ggggagccgt tggacgaggg gcgggtggag cggttgttca ccagggcgca
    12481 gcggcgggtc ctgatgggtc gagatcgggg gtgcacctac ccggggtgtg gggctccggc
    12541 ggcgtggacg caggcgcatc atgtcgtgca ctggctcgat ggtggcgcga ccgacctgga
    12601 caatgccgcg ctgctgtgcc agcggcatca cacggtggtg catgaccggc ggtacgtcgc
    12661 gcaagtgcgg gcgaccccgg atgagctggg gcggttcgtc gtctgggacc gcaccgtggg
    12721 gtcgtacgac cagtacctgg aggcggtgcg ggcggagcga gcggtggctg atccgcgccc
    12781 gctgacaccg cagcggctgg ctgagctgct cgccgcgatc cgagccgatg atcctgacga
    12841 gcagcactgg gccaggctcg agctcgacct cgaacgtgac gaacacgagc agtggcggca
    12901 gtcgcaggac tgggctgacc tgctcgagag catgcccccg cgagnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnggcgag acgtggctct tgtcggcgta gtactccatc
    13081 gaccacacgt cgtcgtcggt cggggcgatg ttccagtcgc cgcacagcgc caccggggtg
    13141 ccgtcggccg cccagcccgt ggcctgctca cgcaaccggt cgagccacga cagcttgtag
    13201 gccatgtggg ggtcgtccag ggcgcgaccg ttgggcacgt agagcgacca catccggatg
    13261 ccgccacacg tcgccccgat cgcgcgcgct tccgcggtgg ccggctctcc ccagccgggt
    13321 gcaccgtcga agccgacctg cacgtcgtcg aggccgacgc gtgacgcgat gcccaccccg
    13381 ttccactggc tcacgccgtg gtgggcgacc tcgtacccga gcgccctgaa gcggtcgtac
    13441 gggaactgct cgtccttggc cttgctctcc tggatggcca ggacgtcgac ctgcgagcgg
    13501 gccaaccagg cctcgacgcg gtcgatgcgg gagcggatcg agttgacgtt ccaggtggcg
    13561 atgcgcacgc cgtcacccta cgcaacgtcg gggcacaccc gcccgggcgg ctacgcgagg
    13621 atgcgggcct tctgcgtctc gaactcggcg tccgtcagca cacccgcggc gcgcagctgc
    13681 ccgagccgct ccagctgctg gagcatctcc tccgtgcccg aggcctgcga cagggccggt
    13741 ggggctggtg gggcggccac cggggacggg gcagcggcgt ggaccggcgc gtgcgcggga
    13801 gcagaggctg cggcagcggc atcctgtgct gcccatcgct ggctctggcg acgttgcacg
    13861 ttgccgtgca cgcggctggc cacggcgacc ttggcagcgg tcttcaacaa gcccatgggg
    13921 aactccttgg cgtgacgggt gggtggctca gggggtgagg gtgtcgtcgc cgagggcgag
    13981 gtccagctcg acggggtcga ccgcgccctc ggccacgagc ctggcgccgg atgccgccca
    14041 cgccccgaga acgggctgca gggtgaggtc ttcgtagacg aggacggcca tgacggcgtc
    14101 tcccccgagg gagccggcca cgagctcgag gtcgtcgcgg tcgagcaggc cggcgtcggt
    14161 accggcgaag gcggagaggt cggcgccgag gtcgagggac gcagcgggca cggtgacgag
    14221 cgctccatcc tcgccgcggc ggatgaactc gaggtcgagg tcgatgacga gtccgcggtc
    14281 gacgaggtca cgcagggcgg cgaagccagc ggcatccgcc cgaccgtcgg ggaactcgac
    14341 ggcgacgtag gacacgggtc cgaggtggtc cgtgatgtcg agagggctgg cgtcgtggga
    14401 agtcgcagcc gcactatcgc accggggtca ggatttctct gtaccgcgtt cgggtgacgg
    14461 ctcgcgccct gctcgtggcc acggcataca gtcgcgacca tgaccacggc actcgtcacc
    14521 ggcgcgtcgg ccgggatcgg ccacgccttc gcccagcgac tcgcccgcga gggccacgac
    14581 ctcgtcgtcg tcgcgcgcga ccgcgcccgg ctcgagcgcc tcgccgcgga gctgcgggca
    14641 gcgcacggcg tcgacgtgga ggtgctcgtc gccgacctgt ccgaccgcca cgcgacggat
    14701 gccgtgtgcg cgcgtctcgc ggacgccgag cgtcccgtcg acctcctcgt caacaacgcg
    14761 ggcttcggcc tggccggctc cttcctcgac aacgacgtgg cccaggagga ggccgggctc
    14821 gacgtgatgg tccgggccgt catgctgacc tgccacgcgg cggggcgtgc gatgcgggcg
    14881 cgcggccacg gggccatcgt caacgtgtcg tcggtggcgt cgttcatcgc tcatggcacg
    14941 tactcggcgg agaaggcctt cgtcacggtc ttctccgaag ggctcgcgag cgaactggcc
    15001 ggcaccgggg tcacggtgac cgcgctgtgc cccggcttca ctcgcaccga gttccaccag
    15061 cgggcccgga tgaagttctc ggcgctgccc gagttcctct ggctcgacgc cgacgacctc
    15121 gtgcagcagg cgctgaccga cgtcgctgcc ggcaaggtga tctccgtgcc gggggcgcag
    15181 tggaaggtcc tcgcgaccac cgtgcgggcc ctgccccgtc cgctggtgcg tggcgccggg
    15241 gtgcgtgccc tgcaccgctt ccgccgggac tgatgcgacc gggtcggtgc cctacggttg
    15301 acccgtgacc gacatcgccg ctgaccgtgc ccgcctcctg cagatcgtcc gcgacaaggc
    15361 gatcgtccac ggccgggtga ccctgtcctc gggccgtgag gccgactact acgtcgacct
    15421 gcgccgcatc accctcgacg gggaggccag cccgctcgtc ggccgcgtca tgctcgacct
    15481 cacggctgac ctggacttcg acgccgtcgg cggcctgacc ctgggcgccg acccggtcgc
    15541 caccgcgatg ctgcacgccc gggccgcgca gggtggccgc ctcgacgcct tcgtcgtgcg
    15601 caaggccggc aaggcccacg ggctccagca gcgcatcgag ggcccgtcga tcgagggccg
    15661 ccgggtgctc atcgtcgagg acacctcgac caccgggtcg tcgcccctgg atgccgcgca
    15721 ggcggctcgc gatgccggcg ccaccgtcgt cgcggtcgcc acgatcgccg accgggcgac
    15781 cggtgcggcg gagaagttcg cggatgccgg gctggagtac cggcacgtct tcggcctcgc
    15841 tgacctcggc ctcgcctgaa acagcagcag ggcccgacgg cgaaccgtcg ggccctgcgc
    15901 cgtgcggagc tcagtgccgg tcggcgaact ccttgatctc gccggaggcc agacgtgccc
    15961 ggtagtgcga gaactcgcgc cggatgacgt tgcgcaacag gtagagaccg atgaggttgg
    16021 ggatgctcat catgaagatc atcgagtccg agaagtcgat gaccgagccg aggctggcga
    16081 ccgaacccag gacggtgaag ccgaggaaga tgaccttgaa gacgttctcc gccagggggt
    16141 tgtcaccgaa caggtacccg gtggccttca tgccgtagta cgaccagctg atcatggtgg
    16201 agaaggcgaa gagcaccacg gcgacggcga ggaccttcgg gaaccacggc aggaccgagg
    16261 cgaaggcgtt cgaggtcagg gccacaccgc cgagttcgga cgctgcgggg tcgaggtagg
    16321 ccttggtgat gatgatcgtc aacgccgtca tcgtgcagat gacgatggtg tcgatgaacg
    16381 gctcgagcaa cgccacgaag ccctcggtgg cgggctcctt ggtgcgcacc gcggagtggg
    16441 cgactgacgc cgaacccaga ccggcctcgt tggagaacgc cgcccgctgg aagccgacga
    16501 tcagggcgcc gatgacgccg cccgctgcgc cctcgccggt gaaggcgccg tcgaggatcg
    16561 agccgaacgc cgccgggatc tccccgaagt tgccgaggag gacgatgagg cacgcaccga
    16621 ggtagagcac tgccatgaac ggcacgatct tctcggtgac cttcgcgatg ctcttgatgc
    16681 cgccgatgat caccgcgccg acggcggcgg cgaacagcag gcccatgatc cacgtgttgc
    16741 ggccgaggaa gctctcctcg ccgccgacct ggctgacgat ctgggcggtg gcctggttgg
    16801 actggaacat gttgccgcca cccaccgcgc caccgatcat gcagaccgcg aagaacgcgg
    16861 ccaacccctt gcccaggccc ggcttgccga ggtccttcac ggcatcccgg aggtagtaca
    16921 tcgggccacc ggagacggtt ccgtcggcgt gctcctggcg gtacttgacc ccgagcatgc
    16981 actcggcggc cttcaggctc atgccgagga agcccgcaac gatcatccag aacgtcgcgc
    17041 cgggaccgcc gatggcgatg gcgatggcga caccggcgat gttgccgagg ccgacggtgc
    17101 cggacaccgc ggtggcgagg gcctggaagt gcgacacctc accggcgtcg ttggggtcgg
    17161 agtacttgcc gcgcacgagg tcgatcgcgt gcttgaagcc gcgaacgttg atgaagccga
    17221 gccagatcgt gaagaagatc gcggccgtga tcagccagat gacgacgatg cggatcgtgt
    17281 actcgccatt ggcggacggg ccgtagaaga ccagaccggt gatccacccc gacaccttgt
    17341 cgaacagggt ggcgtccggt gcgaactcgg gcaccgggct cgggaaggtg atcggcttcg
    17401 gtgacagcgc gatgatgatg gcgtagaaca cggcgacgac cgcgccgatg atgccgaggg
    17461 tcttggtggc cgaccccatc tgcggggtct tgctgtcgcg catgctgttc tctcccatca
    17521 cggcaccacc gtcacgggaa cgctggcgtg ctgggccagg cggttggcca cgctgccgaa
    17581 gacggcgtcg cgcagccccg agtcgccggt gcggccgacg atgatgtgca cggcgccgga
    17641 ctccgcgctg atgtcgagca acgtctcgga cgccttgccg tgacggacga cctcctcgtg
    17701 gggcaccccg ccccgctcgc agatctcgac catgggcgcg atgacctgtt cggtcgccgc
    17761 ctcgagttca cgctgccgtt cacggtgccg gtgctcgttc tcgctggcgg tctggaacga
    17821 gaacggtgtc caggggatca cgtggacgag gacgatcttc cagtggttct tctgggcgcg
    17881 atcaacggcg aactccaccg cgcgggtgga ggcctcactg ttgtcgacgc cgacgacgat
    17941 ggtgtccatg ggagggccta tctgtcggtg cggactggtc aggtccaagg caggctaacg
    18001 cagtgtgacc gcggacacac ctgctgggaa ctacgcttga ggcagttacc cgtctcctcg
    18061 aggagtccca tgacagccgc ccttgtcatc gtcatcgcca tcgtggtgct gctcatcgcc
    18121 gtcgtcggct gggccatcgc gacctacaac ggcctcatca agctgcgcaa cctggtgcag
    18181 gaggcctggc gccagatcga cgtcgagctc acccgccggc acgacctcat cggcaacctc
    18241 gtcgagacgg tcaagggcta cgcagcccac gagcgcggca cgctcgagga tgtcatcaag
    18301 gcccgagctg gtgccatggc gcccggccag tccccggcgc agcaggccga gagcgagaac
    18361 atgctgagcc aagccctcgg acggctgctc gccatcgccg aggcctaccc cgacctgaag
    18421 gccaaccaga acttcatggc gctgcaggcc gagctcacct cgaccgagga ccggatcgcc
    18481 tcggcccgcc gctactacaa cgccacggtc cgcgacctca acaccaaggt cgagacggtg
    18541 ccgagcaaca tcatcgccgg cgtgttcaac atcgcccggg ccgagtactt cgaggccgtc
    18601 ggggaacagc gcgaggcccc caaggtcgac ttcgggcagc gtgaggccgt catcccgccg
    18661 ccgagcgggt atgccggtgg ctccggcaac ggcccccagg acacgccccc cgcaccccag
    18721 gcctgactcc gcgggtcccg ggtccaccgg gtggtggacc cggcatccac tgcgtgggtg
    18781 ttccagccca cgctcccggg cgggcagtct ggacccatgg acgacgcaat cacgatcagg
    18841 ggcctgcgca aggcctatgg cgggcgggcc gccgtcgacg ggctcgacct cgacatccgc
    18901 acgggggagg tgttcgcgct cctcggaccc aacggggccg ggaagacgac caccgtcgag
    18961 atcgccgaag gatttcgcag ccgtgacgcc ggcgaggtcc gcgtgctcgg cacggacccc
    19021 gagggagcgg ggcgttcctg gcgcaaccag atcggcatcg tcacgcagac ctcggcgggc
    19081 ctggacctgt tgaccccccg cgaggcactc gcgagcacgg cgaaggtcta ctccggcccc
    19141 cgcgacgtcg acgaggtgat cgccgccgtc gggctcgccg acaaggccga cacccgcatc
    19201 gtggggctga gcggtggcca gcgacgccgc ctcgacgtcg gcctggggat cgtcggtggg
    19261 cccgagctga tcttcctcga cgagccgacc accgggttcg acccgcaggc ccggcgcgac
    19321 ttctggaccc tcatccgcgc gttggccgac cagggcacga cgatcctgct cacgacccac
    19381 tacctggacg aggccgaaga gctcgcggac cgggtcggcg tcatcgccgc cggtcgcctc
    19441 ctcgccctcg acacccctgc ggcgctgggc gggcggggca cgtcggaggc cacggtcacc
    19501 tgggaggagg acggcatccg acgaagcctg cgaaccgcga cgccgaccgc gcaggtgagc
    19561 cggctcgcag cccggatgcc gggtggtgag gtccctggcc tcaccgtcac cagaccatcg
    19621 ctcgaggaca cctacctcac cctcatcgcc ccccacatca ccgacgacac cacggaggtg
    19681 gcgtcgtgaa cgcgctcgcc ctcgggctgg accgcaccat cctcgagctg aagatgtact
    19741 cgcgcgagaa ggagtcggtg ttcttctcct tcctgttccc gatcctgctg ctgagcctgt
    19801 tctcggtcat cttctcggcc cagttccagg aaggggcggg ccagggcggg atgaccgcgg
    19861 gcctgttctt catgccgggc atgctctcgg ccggtgtgct cctcacgagc ttccagacga
    19921 tggccctgtc gatcgccacc gaacgtgacg acggcacgct caagcggctg cgcgccaccc
    19981 ccatgccgtc ggtggcgtac ttcctgggca aggtcggact cgtcggcgtg acctccgtgc
    20041 tgcagacggt gttgctcctc gtcgtggcga cgctgttctt cgacgtcgac ctgccgaccg
    20101 accccgagcg ctgggccgtc ttcgtgctcg tcttcgggct cggcgtcttc agcggcacgg
    20161 tgctcggcat cgcgtactcc tcgctcgcga cgtcccgctc gatcggggcc gtcgtcatcg
    20221 ggccgatgct cgtgctgcag ttcatctccg gcgtgtacat cgcgttcgac aacgtgccca
    20281 cgtggttgca gcaggtcgcc tccctgttcc cgctgaagtg gatcgcgcag ggcatgcggt
    20341 cggtgttctt cccggccgag atggcggccg gcgagatggc cgggtcgtgg gagaccggca
    20401 agacggccct cgtcctcctg gcgtggggca tcgcgggcct ggtcctgtgc atcacgacgt
    20461 tccgctggtt caagcgcgga acgacgtgag ccgagggcga cgatgatggt gtccgtgagc
    20521 acgaccgagc ggacgtcgcg cgaggagttc ggcgcgatgt ggcgccgcca gcagcccttc
    20581 tggcacgccg ccatggcagt ggtctggggt gccgcggtcg tcgtcacgct gctcgacgac
    20641 cccgggccgt gggggcgcgg cccgtcgctg gcgctgctcg ggctcatggn nnnnnnnnnn
    20701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gaccatcgac gagctgcgcg ccgcgcaggc
    20821 agagctcgct gcgtccgaac gcgaccgcgg cgtgcacgag gagcgcgagc ggctctcccg
    20881 cgagatccac gacaccctcg cgcagggctt cacctcggtc gtggcgctct cccgcgcggc
    20941 tgacgcggca ctggcccgag gcgacgtcga tgcggcgcgc gaccggttgg cgctcatcga
    21001 gcgcacggca tccgacaacc tcgacgaggc ccggctgatc gtcgcggagc tcacgcccgg
    21061 ccacctccag tcgcgcaccc tgggagaggc gctgcatcgt ctcggggcgg ccgtgagcag
    21121 cgagaacggg ctgcgggccg atgtgagagt gacgggggag cccgtgccgc tcgggggcac
    21181 cgccgaggtg gtcatcctgc gcaccgccca ggaggcactg tccaacgtgc ggcgtcatgc
    21241 gggcgccggg cacgctgagc tgatcctgac ctacgagcac cccgagcagg tcgtgctcga
    21301 ggtgcgcgac gacggccggg gcttcgaccc ggccgctgcg cgatccggct tcggactcga
    21361 cggggtgcag gcccgggcgg ccgaggtcgg tgggtcggtg cggctgcgca gcgaaccggg
    21421 cgcgggcacc accctgcggc tcgaggtacc gcggtgatcc gcctgctggt cgtcgacgac
    21481 caccccgtcg tgcgggccgg catggtcgcc gtgctcagcg aggaggccga cttcgaggtg
    21541 gtgggcgagg ccgcgaacgg cgccgaggcg ctggcactgg taccccgact gatgcccgac
    21601 gtcgtcctca tggacctgcg gatgccggtc atggacggtg ccgacgccac ctcacggatc
    21661 gtcgcgcagt ctgacgcacc ccaggtgctc gtgctcacga cctacgacac cgacgccgac
    21721 atcgtgcgcg cggtcgaggc aggcgctcgt ggctacctgc tcaaggacgc gcccaccgcg
    21781 gtgctcgccg acgcgatccg ccgggctgcg cggggggaga ccgtgctcgc cccgccggtg
    21841 gcggcccgcc tggccgatcg gctgcgggcg ccctcccggc ccgagctcac cgcgcgagag
    21901 ctcgacgtgc tgcgcctggt ggccatcggg ctgtcgaacg gcgacatcgg ccgggagctg
    21961 ttcatcggtg aggcgaccgt gaagacccac ctcatccggg tgttcgccaa gctggaggtg
    22021 accgaccgga cggctgccgt cacggccgcc tacgcgcagg gcctgatcga cctgccccgc
    22081 ccgtgacccc tacagggtgc cgtcgcgcac gtcgtcgacg acctcgtcga tctcgtcgat
    22141 gacggtcttg gtcgccgagc ggtgcctgag gaactcgaac accatgggca gcaccgagat
    22201 ggccacgatg agcagcaccg ccgcctcgag gttgtcgtgc acgaagtcga tgccgccgag
    22261 gaagtacccg agcagggtga tgccggtcgc ccagagaacc gcgccgacag cgctccagac
    22321 gaagaagtgc cgacggttca tccggctgac gccggcgacc acggtgatga acgtgcgcac
    22381 gatgggcacg aaccggccga tgaccagcgc cttggcgccg tacttctcga agaagtcgtg
    22441 ggtctggtcg aagtacttct tcttgatgaa ccggccgtcg cgctcgtaca gcggcgcccc
    22501 gatggcccga ccgatctcgt agccggtgac gttgcccgcg aaggcggcgg cgctgaggat
    22561 ggcgcaggcc acgacgatgt tgaggtcgat gtcaccgcgg gcgatgaaca ggccgaccgc
    22621 gaacagcagc gagtcgccgg ggaggatggg gaacagcagg ccgcactcga tgaagacgat
    22681 ggccacgctc acccagaaga actgctctcc gaagcggtcg aggagccact gggggtccat
    22741 ccagtcaggt ccgagggaag gcatcacgcc ctcaagagta ggtcgtccgg ccgcccggcc
    22801 ccgcccgctc gccgggcggg cgcaccctca cgtcagggcc gcggctacat tgagcaccga
    22861 tccgggccga tcgggacggc tcgcaccgac agtgaaggaa cacccatgcc gaccacccgc
    22921 gccacccgcg ccacccgcct gttcggggtc gccctcaccg ggtccgccct cctcgcgctg
    22981 gccgcctgtg gcagccccac cgacgaaccc gccgcctcgg cctctggtga cgccacggca
    23041 tccgccgcca cctgcacccc cgagacgatg aagacgctga cggccggcac cctgacgatc
    23101 ggcaccgaca agccggccta ccagccgtgg ttcgtcgacg acgacccgag caacggcaag
    23161 ggctacgagt cagccgtggc ctacgcgatc gccgacgagc tcgggttcgc caaggacaag
    23221 gtgacctggg tcgtcgcgcc gttcaacacg gtcgtcgcgc cgggcaagaa ggcgttcgac
    23281 ttcgacgtga accaggtgtc gatcagcgac gagcgcaagc aggccgtcga cttctcgagc
    23341 gggtactacg acgtgcgcca ggcagtcatc acctacaagg gcagcccgat cgacggcaag
    23401 acgaccgtgg cggacctcaa gggcgccaag ctcggtgccc aggtcggcac cacctcgtac
    23461 aacgccgcga aggaccaggt gcagccctcg gctgcggtcg cggtcttcga caccaacgac
    23521 ctcgccgtgc aggccctgaa gaacaagcag atcgacggga tcgtcgccga cctgccgacc
    23581 gcgttctaca tgacggccgc ccagctcgac gacggcgtca tcgtcggtca gctgcccctg
    23641 cagggtgagc ccgagcagtt cggtgcggtg ctcgacaagg gcagcccgct cacgggatgc
    23701 gtcacccagg ccgtcgacac cctgcgcgag aacggcacgc tggacaccct cgtcaacacc
    23761 tggctgtcca cccagggcgc gcccgagctg aagtagcagc cgatcgtgca ggacccttcg
    23821 cagcagctgg ggcccacgga gtcgacgggg gaacccgtcg aggaccggtg ggtcccgtct
    23881 gcgctggagc aggagcgcgc ggcctaccgc tcggcccgca cccgccgctc cgccgtcgtc
    23941 gcgacggcct cgacgctcgc gatgctgggc gccatcgtcg ccctcgtcgt gagctcgccc
    24001 gggtggcccc gcgtcaagga gaccttcttc tcctgggaca aggcggtcga ctccctcccg
    24061 gcggtgctcg acgggctctg gctgaacatc cgcgtcatgc tcgtgtgtgc ggtgctcatc
    24121 gcgatcctcg gcctcaccct ggccgtcatg cgcaccgtgc ggggtccggt ggccttcccg
    24181 ctccgcctgt tcgcgacggc ctacgtcgac ctgttccggg gtctgccgct gctgctcgtg
    24241 ctcttcctcc tcggcttcgg cgcccccgcg ctgcagctgt cgggggtgcc gaactcggcg
    24301 ctgttctggg gcgggtgcgc gctcgtgctg tcgtactcgg cctacgtggc cgaggtgttc
    24361 cgcgccggca tcgagtccgt gcacccctcg cagcgggcgg cggcgcgctc gctcgggctg
    24421 agctacgggc agaccctgcg gcacgtcgtg ctcccgcagg cggtgcgccg ggtggtgccc
    24481 gccctgatga acgacctcgt ctcgttgcag aaggactccg gcctcatcgc cgtgctcggg
    24541 gtcatcgacg ccatccgggc ggcgcagatc gagacggcca gcgacttcaa ctacaccccg
    24601 tacgtcgtcg ccggtgcgct cttcgtctgc ctgacgatcc ccatggcgcg cttcaccgac
    24661 tgggtggccc ggcggcaggg cttccacggc gccgggggcc acctgtgagc acccacgtga
    24721 ggatgccgtc atgagcctgc tcgccctcga gggggtccgg aagtcgttcg ggaagaacgt
    24781 ggtgctccag gacatcgacc tgaccgtcga gcgcggccag tgcgtcgtcc tgatcggcgc
    24841 cagcggctcg ggcaagtcga ccctgctgcg gtgcgtcaac ctcctcgagg tcgtcgacga
    24901 cggtcgcatc acgctcgacg gcgacgacat caccgacccg cgcatcgatg cggatgccgt
    24961 gcgcgcccgg atgggcgtgg tcttccaggc ctacaacctc ttcccccacc tgtcggtggt
    25021 ggacaacatc accctggccc ccatccgggt gcacggtgtg ccgcggggtg aggcccggga
    25081 caaggccatg gcgatgctcg accgggtggg tctgcgagac cgggcggggg cgcgccccga
    25141 cgacctgtcg ggtgggcagc agcagcgggt ggccatcgcc cgcgcgctcg tcaacgaccc
    25201 gaccctcatg ctgctcgacg aggtgacgtc ggcgctggac ccggaactcg tcggggaggt
    25261 gctcgacctg ctgcgatccc tcacgcagga gggcatgacg atgatcctca acacccacga
    25321 gatgggcttt gcccggcagg ttgctgacac cgtgtgcttc ctcgacggcg gggtcatcca
    25381 cgagcagggg ccgcccgagc aggttctcgg tgacccgcgc gaggagcgta cgcgccagtt
    25441 cctgtcgcgc gtccacgcat gaccgggtac gtgccggcat gagcggccac gacgagcccg
    25501 ccgacgtggg ggtggggccg tggcagggtc cgtggcccga cggggaccac ttcgaccccg
    25561 acttgttgcg cgagggcgac cggcgcaacg tcgccgaccg gtaccgctac tggaccatgg
    25621 atgccgtcgt ggccgacctc gacacccgtc gccacccgtt ccacgtcgcc atcgagaact
    25681 ggggccacga cttcaacatc ggctcggtc
//
LOCUS       DAOSOC010000016        25662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1917_length_25662_cov_10.755965, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000016 DAOSOC010000000
VERSION     DAOSOC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 25662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25662
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1917_length_25662_cov_10.755965"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..1957
                     /gene="glmU"
                     /locus_tag="PL014_02505"
     CDS             476..1957
                     /gene="glmU"
                     /locus_tag="PL014_02505"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOT55997.1"
                     /translation="MSTPRPAAVIVLAAGEGTRMKSSTPKVLHRIGGLTLVGHAIRAA
                     RETGAEHVGVVVRHERDRVVEQCTALDAGLVIADQDDVKGTGRAVECGLEALPADLSG
                     TILVTYGDVPLLTGDTLVQLTVSHQESGSAVSVVTAHLDDPTGYGRVVRDAQGRVERI
                     VEHKDAIADELALTEINSGIYAFDAQVLRDALAEVGTDNAQGEKYLTDVISIARDAGR
                     PVRAHVIGDRLQTEGVNDRVQLAELGRELNRRVCERHMRAGVTIVDPATTWIDVDVSI
                     GQDATILPGSQLLGATSIGPDAVIGPDCTLTDTEVGAGASVTRAVTNLAVIGDGATVG
                     PYSFLRPGTRLGARGKIGGFVETKNADIGEGAKVPHLTYAGDVTIGAGANIGAGTIFA
                     NYDGVAKHHSVIGSHSFVGSNSVVIGPVDVADGAYVAAGSTLTQDVEPGEIAVARGQQ
                     RNVSGWVARARHGTATAAAAAAAQKAGERHPSPATTTAEGDQQ"
     gene            1954..2955
                     /locus_tag="PL014_02510"
     CDS             1954..2955
                     /locus_tag="PL014_02510"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOT55998.1"
                     /translation="MSSNRPPSANIRKRPKKQLMLFSGRANPELAQEVAAQLGTELVP
                     TSAYDFANGEIYVRFEESVRGSDAFVIQSHTNPINECIMEHLLMVDALKRASAKRITV
                     VMPFYGYARQDKKHRGREPISARLIADMFKTAGADRLICVDLHTDQIQGFFDGPVDHL
                     MAIPILSEYVATKYGDQDLAVVSPDAGRIKVAEQWSKRLGGAPLAFIHKTRDINRPNE
                     MVANRVVGDVRDRVCVLVDDMIDTGGTIAKAADALKADGAKAVIIAATHAILSDPAVE
                     RLRDCSAVEVIVTNTLPIPPEKQIDGITVLSIAPLISAAVKEVFEDGSVTSLFGGRA"
     gene            complement(2966..3520)
                     /locus_tag="PL014_02515"
     CDS             complement(2966..3520)
                     /locus_tag="PL014_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HOT55999.1"
                     /translation="MIFTYDTDMTLMHAAALVNTGPALGSEHREDLPDVAALVEWMHG
                     WQWTGRHPSTEAEADAVRRLRPRIRQIWGLPEDDLVDATNALLREGRALPQLVRHGDF
                     DWHIHATPDDAPIADRIAVEVGMALVDVIRAGAIDRLKVCAGDDCDDLIIDLSKNRSR
                     KFCDGTCGTRANVAAYRARKAADT"
     gene            3649..4650
                     /locus_tag="PL014_02520"
     CDS             3649..4650
                     /locus_tag="PL014_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT56000.1"
                     /translation="MRRHVGLGLVAMLASCAAFGTSGPFAKSLMTAGWSPGAVVLIRI
                     AGAALVLAPFGLWSMRGRWGTLRSELPLAAFYGTLAVAAAQLGYFQAVSRMPVGVALL
                     IEYLGIIIVVLWVWALTRRAPHRLTGVGIVLALAGLALVLDVTGQSTPSLVGVLWGLL
                     AAVGLAGHYVLAGRPTALPAVAFAGVGLTSGAVVLALAGLIGVLPMEAGASTVVIAGT
                     SAPAWVAFAELVLIAAALAYVLGILGARHLGSTLASFVGLTEVIFAVAFAWVLLGELP
                     GLIQLVGGLVLLSGVVAVRLGERDEARAASHTTTHAGTPGGHDEPARPDFEVHSPVA"
     gene            4860..5495
                     /locus_tag="PL014_02525"
     CDS             4860..5495
                     /locus_tag="PL014_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOT56001.1"
                     /translation="MSDLTLNAETRTQFGKGAARKIRRDHKIPAVMYGHGADPVHITL
                     AAHETMKALKTSNALLTIVLDGKEQLALAKDVQRDPIKPVIEHIDLVVVRKGEKVTVD
                     IPVHIEGDAAPDTMVTTDSTSLELEVEATHIPEGITISVEGLEAGTQILAGQVELPKG
                     ATLITDAETLVVNVTAQVSEEALEAELAEAEAEAGIEREESDEAAASDSDA"
     gene            5617..6219
                     /gene="pth"
                     /locus_tag="PL014_02530"
     CDS             5617..6219
                     /gene="pth"
                     /locus_tag="PL014_02530"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT56002.1"
                     /translation="MSDSGPWLVVGLGNPGPTYAGNRHNVGAMVIDELARRTSSSLRT
                     HKARAVAAQVRLGAGPGGVPGPSAVIAVPNCYMNESGGPVSALVRFFSVTPERVVVVH
                     DELDLDEGTIRLKRGGGEGGHNGLRSISNSLGTKDYHRVRVGIGRPPGRMDAADYVLR
                     DFSPTQRKELPFLLDDAADAVESLVTLGLLDAQQKVHAPR"
     gene            complement(6241..6618)
                     /locus_tag="PL014_02535"
     CDS             complement(6241..6618)
                     /locus_tag="PL014_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT56003.1"
                     /translation="MIIDVDPSGAVPVYEQVRTQLAEMIVTAALPQGFRLPTIRQLAA
                     DLALAPGTIARVYRELEAEGLVVSRVRHGTVVAAVPERHLADPTTAVAAAARTYAGAS
                     RRLNLSLDDAIAALREQWAQLSG"
     gene            complement(6615..>7045)
                     /locus_tag="PL014_02540"
     CDS             complement(6615..>7045)
                     /locus_tag="PL014_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56004.1"
                     /translation="WWGWVAYTGALVVTCALTAGAVMRRPSGFVDSDVRDADDALRTH
                     ALGVLVGSAIAASYPALTGFALLAAHPDGAPTSATAPWALLIMVAALLSGVWVAVWGP
                     SARGRRLSTGTVEATQPVHAVPAGRCDLSAPSGQSEPPPS"
     assembly_gap    7046..7145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7346..10954
                     /gene="mfd"
                     /locus_tag="PL014_02545"
     CDS             7346..10954
                     /gene="mfd"
                     /locus_tag="PL014_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT56005.1"
                     /translation="MSSLSALRSHVAALPGTQRLQEALSVPGAEVAATAPPGARSALI
                     AALAAAASDRAATTPVLAVTATGREAEDLQAALEASLGSGRVGLFPSWETLPHERLSP
                     RSDTVGQRLSVLRRLAHPDAGDASHGHLAVVVAPVRAVLQPIAKGLGDLRPVALQAGD
                     ERPLEQVVEDLAAAAYARTDLVERRGEFAVRGGILDVFPPTEEHPLRLEFWGDTVEEI
                     RWFKVADQRSLEVAEHGLWAPPCRELLLTDTVRERAAALADRLPGAVDLLEKLAHGIA
                     VEGMESLSPALVDGMETLLDVLPQGSLLVTCDPERVRTRAHDLTSTSQEFLEAGWANA
                     ASGNVVPIDLQSVLGTASFRTLAELRAQARDLGVRWLDLTPFAGDEDGDVLDLGLEVS
                     PTFRGDTDAAVTHLRALVADNWSVLVATEGPGLAKRVAEVFAEHDVPSRLVSAGGDAG
                     SGGAHPASGVVTVTTGAAGPGFLVPAQRLAVITESDLTGSAGGSGTSTKDMRRMPSRR
                     RNQVDPLALQPGDFVVHEQHGVGKFVEMVQRTVATATREYLVLEYAPSKRGQPGDRLY
                     VPTDQLDQVTRYVGGEQPTLSKMGGSDWQQTKSRAKRYVKQIAAELIRLYSARMATTG
                     HAFSPDTPWQRELEDAFAHIETPDQLSTIDEVKADMERSVPMDRLVCGDVGYGKTEIA
                     VRAAFKAVQDGKQVAILCPTTLLVQQHANTFAERYAGFPVVVKALSRFQTDKEAKAVL
                     AGLADGSVDLVIGTHRLLSKEVQFKDLGLVVIDEEQRFGVEHKEQLKALRTSVDVLAM
                     SATPIPRTLEMAVTGIREMSTLATPPEERHPVLTYVGAYDEKTVTAAIRRELMREGQV
                     FLVHNKVSTIDKAAARLRELVPEARVAVAHGKMGEHQLEQVVVDFWEKRFDVLVCTTI
                     VETGLDISNANTLIVERADRLGLSQLHQLRGRVGRGRERAYSYFLYPPEVPLSETALD
                     RLQTIASNTDLGSGMRVAMKDLEIRGAGNLLGGEQSGHIAGVGFDLYVRLVGEAVASF
                     RGESDETPTEIKIELPVDAHLPHDYVPHERLRLEMYKKLAAVVDAAELAEIEAELVDR
                     YGTPPQAVVNLLEVARLRTVARAAGIADISVQGNHVRFGPVELRESQELRLMRLHPGS
                     IIKPALGTILVPKPMTARVGGRPVRDREVLSWAGEVISSVVMDNVAQAAAVAASRA"
     gene            10982..11518
                     /locus_tag="PL014_02550"
     CDS             10982..11518
                     /locus_tag="PL014_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56006.1"
                     /translation="MTLSVRRSLAVLALGAALVSAGCSVNRAETAAVVDGNVITEGQL
                     QVAMAQINSMEPALLQEPLTPSGALTALVQAPVVLDVLGDKGIVVSDTLATREAQGRG
                     VANPSGETLQVIRLASAITAAQQSGQITEADGAAITEAMGALDVEVNPRYGTFAPETA
                     SIQLTAPEWIKAEEIPAP"
     gene            11515..12471
                     /locus_tag="PL014_02555"
     CDS             11515..12471
                     /locus_tag="PL014_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HOT56007.1"
                     /translation="MSAVPGAPSGRLGMLVTSPRVAPGLLSHGAWQVVQGYAVRLARS
                     FDEPVAQAVEEAGFTVEQVGDASPPELARRLVDLAGAVSVVWLGSADGDPGLADAVAA
                     EVSRLATPPEVELVVGSWDVQGGRLLDVVATMDRLRSPGGCPWDAEQTHESLAPYLVE
                     EAHEVLDAIAGGDRGHLAEELGDILLQVVFHARVAEEAGESAFDIDTVAGLLVDKLVR
                     RHPHVFADGDASTPAEVEAAWESIKAVEKAGVGGDDYRADLLHGIPTSLPGGLAADKV
                     LARVRRRGLTPDPDAVARVERARAALAAAESEVRAALEGLQR"
     gene            complement(12561..13487)
                     /locus_tag="PL014_02560"
     CDS             complement(12561..13487)
                     /locus_tag="PL014_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56008.1"
                     /translation="MEGLGLPSELTSRPFTVAQGREAGLRLRTLRSTRLHVPTHGVRM
                     ISTPATVQEKAAAFALGLPDDAAFSHVSAAQLLGLPLPTALEAPEVLDVMRPTSRAQV
                     RRDGCRGHRGLERREVIDVQGLRVVAPADTWCDMGELTSPALAIDDLVVMGDAVVNRL
                     EPEGIATLRSALDSRTRPRGRARLMAAGGLIRVGSRSPMETRTRLMFHRAGFPEPELN
                     AEVLDCHGGWLLDGDFFWRRQRVIGEYQGADHASRKRRSADSMRSGLATDEDYQVIEI
                     YAEDVYGRARRCTLLRRFARAMDIDPATLTIE"
     gene            13690..14838
                     /locus_tag="PL014_02565"
     CDS             13690..14838
                     /locus_tag="PL014_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT56009.1"
                     /translation="MKLTAREHEIVTLLRADPMLDAAALAERIGSTRAAVSVHLSNLM
                     KKGVVRGRGYLLRPEADTVLVVGGAVLDTKVRTAGPPVLGTSNPGASSSTVGGVGRNI
                     AENLARLGRPTVLVAPVGDDPAGEAVADRTTAAGVDCGHLVRSPHPTGTYVAVLADSG
                     DLHIAVADMRAADELAVADLASVPGLLTGADALVVDANLSESVVRWLLNAAAEAGVLS
                     ILDPVSVAKATAVAPVLDGTIPVHTVTPNVDELSALVGAPVEPDVDAVRAAAALLHER
                     GVSHVWVRRGTAGSLLSIATASTPRVVLVDAPPTDVADVTGAGDSMTAGFVNALLDGA
                     DVVEAARHGQVLAALTCASPDTVRDDLTPALVAAHLTPSQPSTKELHR"
     gene            14835..15770
                     /locus_tag="PL014_02570"
     CDS             14835..15770
                     /locus_tag="PL014_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOT56010.1"
                     /translation="MTTSTSTTPHPLLKLDPEVAQALADGTPVVALESTIISHGMPYP
                     ANVAMAREVEQIVRDGGAVPATIAVLNGTPTVGLSTDELEMLGSRDDIRKVSVRDLPY
                     VVATGTHGATTVASTMRIAGLAGIRVFVTGGLGGVHQGAESSMDVSADLTELSRTEVA
                     VISAGVKSILDIGRTLEVLETLGVPVVGYGTDDFPSFFSRTSGLRTPMRLDTPQEIAA
                     LMEATWGLGLGSGISIANPVPAESEMAHEDIDSVIARALADADALGVTGKDITPYLLG
                     RLVELSDGRSLETNLALVRNNAVVGTAIAVAYAAR"
     gene            15885..17177
                     /gene="eno"
                     /locus_tag="PL014_02575"
     CDS             15885..17177
                     /gene="eno"
                     /locus_tag="PL014_02575"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT56011.1"
                     /translation="MASIEAVGAREILDSRGNPTVEVEVALDDGTLARAAVPSGASTG
                     AFEASERRDGDKDRYLGKGVLKAVDAVIDDLGPKLLGFEASEQRLIDAEMLAADGSAN
                     KEKLGANAILGVSLAVARAAADSADLPLFRYVGGPNAHVLPVPMMNILNGGSHADSNV
                     DIQEFMIAPIGADSFREALRWGAEVYHALKGVLKEQGLATGLGDEGGFAPNLPSNRAA
                     LDLILVAIEKAGYTPGEQIALALDVAASEFHDGGVYSFEGGSKTSAEMIDYYAELVRD
                     YPLVSIEDPLDESDWDGWKAMTDRMGNDVQLVGDDLFVTNPERLARGIETGTANALLV
                     KVNQIGSLTETLDAVSLAQRNGYRCMMSHRSGETEDTTIADLAVATNCGQIKTGAPAR
                     SERVAKYNQLLRIEEELDDAAVYAGAEAFPRFARRQQG"
     gene            17297..17728
                     /locus_tag="PL014_02580"
     CDS             17297..17728
                     /locus_tag="PL014_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOT56012.1"
                     /translation="MPVTGWRRLLGGVTGDRRARRALALTAVLGLLVILLGSTVTAYI
                     GQRGDIAALRDKVAAQEQDVAALQAERERWRDPAYVEQQARQRLKFVKPGEKSYTVLD
                     PEPDAGNRDPLAAIASSDEELPWYQGVWESARTADAPGARR"
     gene            17725..18273
                     /locus_tag="PL014_02585"
     CDS             17725..18273
                     /locus_tag="PL014_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HOT56013.1"
                     /translation="MSAGHDLPPVDPADLEAVTRQLGREVRGVAEVAHRCPCGEPDVV
                     RTEPRLPDGTPFPTSFYATCPKLTGALSTLESQGVMREMSERLEHDEALRAAYAAAHV
                     DYLERRAQLGDVPEVAGISAGGMPTRVKCLHVLVAHSLGAGPGVNPLGDEALAMLPQW
                     WQRHPCSADAPGSAAAQGEASA"
     gene            18270..19223
                     /locus_tag="PL014_02590"
     CDS             18270..19223
                     /locus_tag="PL014_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOT56014.1"
                     /translation="MSGAPTRVAGIDCGTNSIRLLIADVDAGGALVDRVRRMEVVRLG
                     QGIDRTGVIAPDAMERTLAMTREYAALCREHGVTAVRFVATSASRDARNAGVFVEGVR
                     AAFGDLDVVPEVVEGIVEAELSFTGATGGLRAAAVPGPYVVVDLGGGSTELVRGTAHV
                     EAAYSMDIGSVRMTERHLHDDPPTADQVAAARADVRAALDVAEREVRLDGVGAVVGLA
                     GSITTITAHALGLTRYDPEASHLARIPADVHREACRSLLAMSREQRAGLGFMHPGRVD
                     VIGGGALVWLEVLDRVLARSPEAVVVTSEHDILDGVTLSLA"
     gene            19375..19707
                     /locus_tag="PL014_02595"
     CDS             19375..19707
                     /locus_tag="PL014_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT56015.1"
                     /translation="MEGTQLVKGVLDLAVLAVVAREDGYGYDVVRRLRSAGLEEVGDA
                     SVYGTLRRLYSAGALTSYVVPSDEGPHRKYYGINAQGRHLLATEGKTWAAFAATMDGL
                     LEQAGVQS"
     gene            19704..20819
                     /locus_tag="PL014_02600"
     CDS             19704..20819
                     /locus_tag="PL014_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56016.1"
                     /translation="MNDAEAMGATTQGGSASATQVLPTGAESAQARSADVRAYVTAVR
                     SWLSDLPSDEVEDLTAGMEADLDERAAETGDRLGALLGEPEAYAAELRAAAGLAPRVV
                     PVVGAGAAGPGLVEALVTLLRARADGILTRWPWLRELRPTWWLARGAALGWLLSMWTG
                     TGRTVLLPLLGAVLSFWLGLTMRRRDPLRPAAWTSLAVANVIAALAVLPMVSTYTQSY
                     LGSDNEYLREGYAPYPESITANGEEVRNLFAYDASGNRLDGVRLFDQAGRPVVIDTNL
                     LFDGSFEDIPTRPDDGMPDVAHDVFPLRWAGHDAWADPRMPWTPPLVLAPVPGVTPST
                     AVSPTPSPTSTASPSASPSATSTESGTPSPSPTVTTP"
     gene            20931..21008
                     /locus_tag="PL014_02605"
     tRNA            20931..21008
                     /locus_tag="PL014_02605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20965..20967,aa:Leu,seq:taa)
     gene            21134..21979
                     /locus_tag="PL014_02610"
     CDS             21134..21979
                     /locus_tag="PL014_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOT56017.1"
                     /translation="MASNPAFNRIEKDAQSGYAGFGGQGYSSPSPMSAQPNMAEMTPQ
                     QLQDMYNGPSAGPVDTRRVTMDDVIMKTLGLFAIVLVTGAVGWTVAAANPGLGMMLWL
                     GGMIGTLVLGLVIAFKKALSVPLILAYAVVEGLFMGAVSQYFNEAYPGIVGQAVLATL
                     STFAGMFLAYKFGLIKVTAKFRRIMTMMIFGYAIFAVVNFIFAMVTPTAFGFGGSGPL
                     GIGISLFAVGLASFTLALDFDSIEQAIRTGAPAKYSWLLAHGLIVTLVWLYIEFLRLF
                     ARLRE"
     gene            21976..22431
                     /locus_tag="PL014_02615"
     CDS             21976..22431
                     /locus_tag="PL014_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56018.1"
                     /translation="MTEHDAHAPAGAGSPAPAVPSVHPGGTFGDPGADAGRPASVDVV
                     ASQARTELDRIRRRWAELPIAAAEQRMPVLRHLLADLAPRTFPRLLALQPPERPPPEV
                     AGAQEVAEVPDLGPAVVVDQLSVLVWDAYAQGNGDGIPDLLTAARRALA"
     gene            complement(22514..23737)
                     /locus_tag="PL014_02620"
     CDS             complement(22514..23737)
                     /locus_tag="PL014_02620"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOT56019.1"
                     /translation="MTEAVIVATARTPIGRAFKGSLKDIRPDDLSAQVVHGLLAKLPG
                     LDPSTIDDLYWGCADPSGRHGSNMARVIAVLAGMDHLPAATINRFCASSTQTMRMAFH
                     AIKAGEGDTFLVGGVECVSQYTSFAGAGGSDADTQNPLFADSAARSAEMAASNATWTD
                     PREAGMLPDIYLSMGQTAENVATARGVSRARQDEWGVLSQNRAEQAIADGFFAREILP
                     VTMPDGTVVSTDDGPRAGVTLEAVSGLKPVFREEGTVTAGNCCPLNDGASGVVVMSDV
                     RARELGLTPLARIVSTGVSGLSPEIMGLGPVEASRQALARAGMSIGDMDLYEINEAFA
                     AQVLPSADDLGMDPERLNVHGGAIALGHPFGSTGTRIMTTLINGLQTRDGQFGLETMC
                     VGGGQGMAIIVERLS"
     assembly_gap    23938..24037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24038..24178
                     /locus_tag="PL014_02625"
     CDS             <24038..24178
                     /locus_tag="PL014_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT56020.1"
                     /translation="DVVAAISSAHGEIFGDIRPASTMVVVAGLLDPRWKVEIELDAIV
                     TD"
     gene            complement(24199..25335)
                     /locus_tag="PL014_02630"
     CDS             complement(24199..25335)
                     /locus_tag="PL014_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT56021.1"
                     /translation="MGRARRARKIAATAAYGGGGIAAAGAALGAAGWGLIKAEAALAR
                     RVIGSPFDSSPDDNGVYGSGAGRPFDIVVLGDSSAAGLGADAPHETVGAIIANGVAAL
                     TGRRVQLTNRSVVGAESSDLERQLANALEEVLRPDVVVIMIGANDVTHRIERSASVRA
                     LEQTVRRIRALGSEVVVGTCPDLGTVQPIAQPLRALMKHWSRDLAAAQTVAVVEAGGR
                     TVSLADLLGPEFERSPHEMFSKDRFHPSAAGYARVASAMLPTVCAALGVWGVDTVDRA
                     PERRRGEGVGPVAVAAGQAVKEPGTEVAPTEIAGQSRGPRGRWAVLLRRRRDAVPPPQ
                     DPVEESPIEASVTTDGSDAVAEGEPSASDLGAPTSDSPASPGSA"
     gene            complement(25381..>25662)
                     /locus_tag="PL014_02635"
     CDS             complement(25381..>25662)
                     /locus_tag="PL014_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT56022.1"
                     /translation="TPEPFDIEEEGTYVESDHRFVNTKQLSWNHGIGETMTALLRRGF
                     EVTGFVEHRSVPWEALPGRMVRDGPHDEWRLTNHPERMPLTYTLQARKR"
BASE COUNT         3621 a         9258 c         8900 g         3683 t
ORIGIN      
        1 ggtggcaccg tcgctcacgg gtggcagcca gcccaggttc tgcacgtgtg ccgtggagga
       61 gaccgacggc gtggtgggac ccggtgccgc gggcgcgggc gcctggggag cggttgcacc
      121 tggcactgcc gcgacggacg gtgccgcgac ggacggtgcc gcgacggacg gtgccgcggc
      181 ggacggtgcc acggcgaccc cgacgaccgt gagggcgagc accagcgccg tgccgacacg
      241 accaagacgg gctcgatggc cccatcggtt ccggtgctgc ggcgtggtcg acggcatcgg
      301 gtgttcctct ccggcctcgc ggcggcatcg ccgggccgtt cccatcatgg cggatgcggt
      361 gtgttcacgg tggcaccacg agccggggcg gcggtgtcgt gccgtgcggt ggtggcggcg
      421 cgggtagcat gacgtcgtcc cgtggcacct cgcttctcat ctggagttcc ctcgcgtgag
      481 caccccccgc ccggctgctg tcatcgtcct cgccgcgggt gagggcaccc ggatgaagtc
      541 ctcgacaccc aaggttctgc accgcatcgg tggcctgacg ctggtcggcc acgcgatccg
      601 ggccgcccgg gagaccgggg ccgagcacgt cggcgtggtg gtccgccacg agcgcgaccg
      661 cgtcgtcgag cagtgcaccg ccctggatgc cgggctcgtc atcgccgacc aggacgacgt
      721 caagggcacc ggccgcgccg tcgagtgcgg cctcgaggcg ctgccggcag acctgtcggg
      781 cacgatcctc gtcacgtacg gcgacgttcc gctgctcacc ggcgacaccc tcgtccagct
      841 cacggtgtcg caccaggagt cgggcagcgc ggtgtcggtc gtcacggcgc acctcgacga
      901 ccccaccggg tacggccggg tggtgcgcga tgcgcagggc cgggtcgagc gcatcgtcga
      961 gcacaaggac gccatcgccg acgagctggc cctcaccgag atcaactccg ggatctacgc
     1021 gttcgacgcc caggtgctgc gcgacgcgct cgccgaggtc ggcacggaca acgcccaggg
     1081 tgagaagtac ctgaccgacg tcatctccat cgcgcgcgat gccggccgcc cggtgcgggc
     1141 ccacgtcatc ggcgaccggc tccagaccga gggcgtgaac gaccgcgtcc agctcgccga
     1201 gctcggccgt gagctcaacc gccgcgtgtg cgagcggcac atgcgggccg gggtgacgat
     1261 cgtcgacccc gcgacgacgt ggatcgacgt cgacgtcagc atcggccagg acgcgacgat
     1321 cctgcccggc agccagctcc tcggggccac gagcatcggc ccggatgccg tgatcgggcc
     1381 cgactgcacc ctcaccgaca ccgaggtcgg cgcgggggcc agcgtcaccc gggccgtcac
     1441 gaacctcgcg gtcatcggcg acggggccac ggtcgggccc tactccttcc tgcgcccggg
     1501 cacccgcctc ggcgcccggg gcaagatcgg cgggttcgtc gagaccaaga acgccgacat
     1561 cggtgagggt gccaaggtgc cccacctcac gtacgcgggc gacgtcacga tcggggccgg
     1621 cgccaacatc ggtgccggca cgatcttcgc caactacgac ggggtggcca agcaccactc
     1681 ggtcatcggc agccactcct tcgtcgggtc gaactcggtc gtcatcggcc cggtcgacgt
     1741 cgccgacggt gcctacgtcg ctgccgggtc caccctgacc caggacgtcg agccgggcga
     1801 gatcgccgtg gcccggggcc agcagcgcaa cgtgagcggc tgggtggccc gagccaggca
     1861 tggaacggcg accgccgcgg cagccgccgc ggcccagaag gcgggcgaac ggcatccgag
     1921 ccctgcgacg accaccgcgg aaggtgacca gcaatgagca gcaaccgacc gccgtcggcc
     1981 aacatccgca agcgacccaa gaagcagctc atgctcttct cggggcgggc caaccccgag
     2041 ctggcgcagg aggtcgccgc gcagctcggc accgagctcg tgccgacgag tgcctacgac
     2101 ttcgccaacg gcgagatcta cgtgcgcttc gaggagtcgg tgcgcgggtc ggacgccttc
     2161 gtcatccaga gccacacgaa ccccatcaac gagtgcatca tggagcacct gctcatggtc
     2221 gacgcgctca agcgggccag tgccaagcgg atcacggtcg tgatgccgtt ctacggatac
     2281 gcgcgccagg acaagaagca ccgtggtcgc gagccgatca gcgcgcgcct catcgccgac
     2341 atgttcaaga ccgccggggc tgaccggctc atctgcgtcg acctgcacac cgaccagatc
     2401 caggggttct tcgacgggcc ggtcgaccac ctgatggcca tcccgatcct cagtgagtac
     2461 gtcgccacga agtacggcga ccaggacctc gcggtggtgt cgccggatgc cggtcgcatc
     2521 aaggtcgcgg agcagtggtc caagcgtctc ggcggtgctc cgctggcgtt catccacaag
     2581 acgcgcgaca tcaaccggcc gaacgagatg gtcgccaacc gggtcgtcgg tgacgtgcgc
     2641 gaccgggtgt gcgtgctcgt cgacgacatg atcgacaccg gcggcacgat cgcgaaggcc
     2701 gccgacgcgc tgaaggccga cggggccaag gccgtcatca tcgcggcgac gcacgcgatc
     2761 ctcagtgacc cggcggtgga gcgactgcgc gactgctcgg ccgtcgaggt gatcgtcacg
     2821 aacacgctgc cgatcccgcc cgagaagcag atcgacggca tcaccgtgct ctccatcgcg
     2881 ccgctcatca gcgcagcggt caaggaggtc ttcgaggacg ggtcggtgac cagcctgttc
     2941 ggcggccgcg cctgagcctg ctacatcagg tgtcggcggc cttgcgggcg cggtaggccg
     3001 cgacgttggc ccgggttccg cacgtgccgt cgcagaactt gcgtgagcgg ttcttgctga
     3061 ggtcgatgat gaggtcgtcg cagtcgtcgc cagcgcacac cttgagccgg tcgatcgccc
     3121 cggcgcggat gacgtcgacc agggccatgc cgacctcgac ggcgatccgg tcggcgatcg
     3181 gcgcgtcgtc gggggtggcg tggatgtgcc agtcgaagtc gccgtggcgc acgagctggg
     3241 ggagtgcgcg gccttcgcgc agcaacgcgt tggtcgcatc gacgaggtcg tcctcgggca
     3301 gcccccagat ctgccggatc cgcgggcgca gccgccgcac ggcgtccgcc tcggcctcgg
     3361 tgctcgggtg gcgaccggtc cactgccatc cgtgcatcca ctcgacgagc gctgcgacgt
     3421 cgggcaggtc ctcgcggtgc tcgctaccca gggcggggcc cgtgttgacc agggccgcag
     3481 cgtgcatgag cgtcatgtcc gtgtcatagg tgaaaatcat cttgactcct gacaattgcg
     3541 gcagctaacg tcatcggcaa cagcctactt tgctcctgac acatccgccg cgaacccgcg
     3601 ccggcagggc gctgtcacga gaaatcgagg aatgtcatgg tcgaggcgat gcggcggcac
     3661 gtcggtctgg gtctggtcgc gatgctcgcg tcctgcgcgg cgttcggcac gtccgggccc
     3721 ttcgccaagt ccctgatgac cgctggctgg agccccggcg cggtggtgct catccgcatc
     3781 gccggcgcgg ctctcgtcct ggcgcccttc gggctgtgga gcatgcgcgg gcgatggggg
     3841 accctgcgca gcgagctgcc gctcgcggcg ttctacggca ccctggccgt cgccgccgcc
     3901 cagctcgggt acttccaggc ggtctcccgg atgccggtcg gcgtggcctt gctcatcgag
     3961 tacctcggca tcatcatcgt cgtcctctgg gtctgggccc tgacgcggcg tgcgccccac
     4021 cggctgaccg gggtcggcat cgtgctggcg ctcgccgggc tcgcgctcgt gctcgacgtc
     4081 accgggcaga gcacgcccag cctcgtcggt gtgctgtggg gcctgctcgc cgccgtcggg
     4141 ctggccggcc actacgtcct cgcgggtcgg ccgacggcgc tgccggccgt cgccttcgcc
     4201 ggggtcggcc tcacctccgg tgccgtcgtc ctcgctctcg caggcctgat cggggtgctg
     4261 cccatggagg cgggtgcctc gaccgtcgtc atcgccggga ccagcgcacc ggcttgggtg
     4321 gcgttcgccg agctcgtgct catcgcggcg gcgctggcct acgtcctcgg tatcctcggg
     4381 gcccgccacc tgggctcgac gctcgcctcg ttcgtcggcc tcaccgaggt gatcttcgcc
     4441 gtggccttcg cctgggtcct gctcggtgag ctgcccggcc tgatccagct cgtgggcggc
     4501 ctggtcctgc tctccggtgt ggtcgcggtt cgtctcggcg agcgcgacga ggcgcgggcg
     4561 gcatcccaca ccacgacgca cgccggcacg ccgggcgggc acgacgagcc cgcccgaccc
     4621 gatttcgagg ttcactcgcc cgtcgcctag actcgcgggg ttgcctcggc gagggacgtc
     4681 gtaccggcca ctggtgtccg gcaggccgcg agagcggtcg acgaatccgt gatcgacgct
     4741 ggcggacgca gatccccttc ggatccgcgg acgaacgcgc gctcatcaca gatcggtgcc
     4801 acgtcccgcg tcgaggacca ctcgcccgca gtcctcccca cagctcaagg agcgccacca
     4861 tgtccgacct caccctgaac gccgagaccc gtacccagtt cggcaagggc gcggctcgca
     4921 agatccgtcg cgaccacaag atccccgccg tcatgtacgg ccacggtgcc gacccggtgc
     4981 acatcaccct cgctgcccac gagacgatga aggcgctcaa gacgagcaac gccctgctca
     5041 ccatcgtcct cgacggcaag gagcagctcg ccctggccaa ggacgtgcag cgcgacccga
     5101 tcaagccggt catcgagcac atcgacctgg tcgtcgtgcg caagggcgag aaggtcaccg
     5161 tcgacatccc ggtccacatc gagggtgacg ctgctcccga caccatggtc accaccgaca
     5221 gcacgagcct cgagctcgag gtcgaggcca cccacatccc cgagggcatc accatctcgg
     5281 tcgagggcct cgaggccggc acgcagatcc tcgcgggcca ggtcgagctg cccaagggcg
     5341 ccacgctcat caccgacgcc gagaccctcg tcgtcaacgt caccgcgcag gtctccgagg
     5401 aggccctcga ggccgagctc gccgaggccg aggccgaggc cggcatcgag cgcgaggagt
     5461 ccgacgaggc tgctgcctcc gacagcgacg cctgagcctg gctcactctg actcaactga
     5521 cgtcggggcc cggtccacac ggaccgggcc ccgacgtcgt ccgcaccgga cggcatcccg
     5581 accgggcggc atccgaccga aaggcatact ggcgagatga gcgacagcgg accctggctc
     5641 gtcgtcgggc tcggcaaccc cggccccacg tacgccggca accggcacaa cgtcggtgcc
     5701 atggtgatcg acgaactggc ccggcgcacc agcagcagcc tgcgcaccca caaggcccgc
     5761 gccgtcgccg cgcaggtgcg cctgggcgcc ggccccgggg gagtgcccgg cccgtcggcc
     5821 gtcatcgccg tgccgaactg ctacatgaac gagtccggtg ggccggtctc ggcgttggtg
     5881 cgcttcttct ccgtcacgcc cgagcgggtc gtcgtcgtcc acgacgagct cgacctcgac
     5941 gagggcacga tccgcctcaa gcgtggtggc ggtgaaggtg gccacaacgg gctgcgctcg
     6001 atcagcaact cgctcggtac caaggactac caccgggtgc gggtcggcat cggccgcccg
     6061 ccggggcgca tggatgccgc cgactacgtc ctgcgtgact tctcgccgac ccagcgcaag
     6121 gagctgccat tcctcctcga cgacgccgcg gatgccgtgg agtccctcgt cacgctcggt
     6181 ctgctcgatg cgcagcagaa ggtccacgca ccgcgctgac cggctgcgtg acggcggggg
     6241 tcagccgctg agctgggccc actgttccct cagggccgcg atggcgtcgt cgaggctgag
     6301 gttgagccgc cgactggcac cggcataggt gcgtgcggcg gcggcgacgg ccgtggtggg
     6361 atcggcgagg tggcgttccg gcacggccgc gaccacggtg ccgtgccgca cccgtgacac
     6421 gacgagccct tccgcctcga gctcgcggta gacgcgggcg atggttcccg gtgccagggc
     6481 gaggtcggca gcgagctggc ggatggtcgg caggcgaaag ccctgcggca gggcagccgt
     6541 gacgatcatc tcggcgagct gggtgcgcac ctgttcgtag acggggaccg ctcccgacgg
     6601 gtcgacgtcg atgatcatga cggtggtggc tccgactggc ccgagggggc ggaaaggtcg
     6661 caacggccgg cagggacggc gtggacgggc tgcgtggcct cgaccgtgcc cgtcgagagc
     6721 cgccggccgc gggcggaggg cccccacacg gcaacccaca caccgctgag cagggccgcg
     6781 accatgatca ggagcgccca gggcgcggtc gccgaggtcg gggcgccgtc ggggtgggct
     6841 gcgagcaggg cgaatcccgt cagcgcgggg taggacgcgg cgatggccga gccgaccaga
     6901 acccccagcg cgtgcgtgcg cagcgcgtcg tcggcatcgc gcacgtcgga gtcgacgaag
     6961 ccgctcggac gccgcatcac ggcccccgcg gtgagtgcac aggtgacgac cagggctccc
     7021 gtgtaggcca cccaccccca ccaggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnaggag tggcaggagg tgttcgacac cgggtcggtc gccggcgatg gtcagcaacc
     7201 acgcctcacg gtccgcaccc agcgtccgtg ggggtccgtc gcgcgcacgt gggacaccga
     7261 cgcggtcggt gtggtcgcct tcaacgtctg tcttcccgac tcgctggact gaggctgtcg
     7321 gtaggcgcga ctacagtgtg cggcgatgtc ctcgctgtcc gccctgcgct cccacgtcgc
     7381 tgcactgccc ggtacccaac ggctgcagga ggccctgagc gtccccggcg ccgaggtcgc
     7441 cgccaccgct ccgcccggcg cccgatcggc gctcatcgcc gcgctcgcgg ccgccgcgtc
     7501 agaccgagcc gccaccaccc cggtgctcgc ggtcaccgcg accggccgcg aggccgaaga
     7561 cctccaggcc gcgctcgagg ccagcctggg ctcgggccgt gtcggcctct tccccagctg
     7621 ggagaccctg ccgcacgagc ggctcagccc gcgcagtgac accgtcggcc agcgcctgtc
     7681 cgtgctgcgt cgccttgccc acccggatgc cggggacgcc tcccacggcc acctcgccgt
     7741 cgtcgtcgcc cccgtccggg cggtcctgca gccgatcgcc aagggtctgg gtgacctgcg
     7801 ccccgtcgcg ctccaggccg gcgacgagcg ccccctcgag caggtcgtcg aggacctcgc
     7861 ggcagccgcc tacgcccgca ccgacctcgt ggagcggcgc ggtgagttcg cggtgcgcgg
     7921 tggcatcctc gacgtcttcc cgccgaccga ggagcacccg ctgcgcctgg agttctgggg
     7981 cgacacggtc gaggagatcc gctggttcaa ggtcgccgac cagcgctcac tcgaggtggc
     8041 cgagcacggg ttgtgggcac cgccgtgccg cgagctgctg ctcaccgaca ccgtgcgcga
     8101 gcgggcagcc gcactggcag accggctgcc cggcgccgtc gacctgctcg agaagctcgc
     8161 ccacggcatc gccgtcgagg gcatggagtc gctgtccccg gccctcgtcg acggcatgga
     8221 gacgctgctc gacgtgctgc cccaggggtc gctgctcgtc acctgtgacc ccgagcgggt
     8281 ccgcacccgc gcccacgacc tcacctcgac cagccaggag ttcctcgagg ccggctgggc
     8341 caacgccgcg agcggcaacg tcgtgccgat cgacctgcag tccgtgctcg gcacggcgtc
     8401 gttccggacc ctggccgaac tgcgagccca ggcccgcgac ctcggggtgc gttggctcga
     8461 cctcaccccg ttcgccggcg acgaggacgg cgacgtgctc gacctcggcc tcgaggtctc
     8521 gcccaccttc cgcggtgaca ccgacgctgc cgtgacccac ctgcgcgccc tggtggccga
     8581 caactggtcg gtcctcgtcg ccaccgaggg gccgggcctg gccaagcggg tcgccgaggt
     8641 gttcgccgag cacgacgtgc ccagccgcct ggtcagcgcg ggcggtgacg ccggcagcgg
     8701 tggcgcccac ccggcatccg gcgtcgtgac ggtgaccacg ggggccgccg gcccggggtt
     8761 cctcgtgccg gcgcagcgcc tcgccgtcat caccgaatcc gacctcaccg ggtcggcggg
     8821 gggctcggga acctcgacca aggacatgcg gcggatgccg tcgcgtcgcc gcaaccaggt
     8881 cgaccccctc gcgctgcagc cgggcgactt cgtcgtccac gagcagcacg gtgtcggcaa
     8941 gttcgtcgag atggtccaac gcaccgtggc gaccgcgacc cgcgagtacc tcgtcctgga
     9001 gtacgcaccc tcgaagcggg ggcagcccgg ggatcggctc tacgtgccga ccgaccagct
     9061 cgaccaggtc acccgctacg tcggcggtga gcagccgacc ctgagcaaga tgggtggctc
     9121 ggactggcag cagaccaaga gccgcgccaa gcgctacgtc aagcagatcg ccgccgagct
     9181 catccggctg tactccgcgc ggatggcgac gaccgggcac gcgttctcgc ccgacacccc
     9241 gtggcagcgc gagctcgagg acgccttcgc ccacatcgag acgcccgacc agctgagcac
     9301 catcgacgag gtcaaggccg acatggagcg ctcggtgccg atggaccggc tcgtgtgcgg
     9361 cgacgtcggc tacggcaaga ccgagatcgc cgtgcgggcc gcgttcaagg ccgtgcagga
     9421 cggcaagcag gtcgccatcc tgtgcccgac gaccctgctc gtgcagcagc acgccaacac
     9481 cttcgccgag cggtatgcgg ggttcccggt cgtcgtgaag gcgctgtcgc gcttccagac
     9541 cgacaaggag gccaaggcgg tgctcgccgg gctcgccgac ggctcggtcg acctcgtcat
     9601 cggcacccac cggctgctgt cgaaggaggt gcagttcaag gacctcggcc tcgtcgtcat
     9661 cgacgaggaa cagcgcttcg gtgtcgagca caaggaacag ctcaaggcgc tgcgcacctc
     9721 ggtcgacgtg ctcgcgatgt ccgcgacacc gatcccgcgc acgctggaga tggccgtgac
     9781 cggcatccgg gagatgtcga cgctcgcgac gccgcccgag gaacgccacc ccgtcctcac
     9841 ctacgtcggg gcgtacgacg agaagaccgt gaccgccgcg atccgtcgcg agctgatgcg
     9901 cgaggggcag gtcttcctcg tgcacaacaa ggtgtcgacg atcgacaagg ccgccgcacg
     9961 gctgcgcgag ctggtgcccg aggctcgggt cgccgtcgcc cacgggaaga tgggcgagca
    10021 ccagctcgag caggtcgtcg tcgacttctg ggagaagcgc ttcgacgtcc tcgtctgcac
    10081 gacgatcgtc gagaccgggc tggacatctc caacgcgaac accctcatcg tcgagcgcgc
    10141 cgaccgcctc ggcctctcgc agctgcacca gctgcgcggc cgcgtcgggc gtgggcgcga
    10201 gcgcgcctac tcgtacttcc tctacccgcc ggaggtgccg ctcagcgaga ccgcgctcga
    10261 ccggctccag acgatcgcga gcaacaccga cctcggctcg ggcatgcggg tggcgatgaa
    10321 ggacctcgag atccgcggcg ccggcaacct gctcggcggc gagcagtcgg ggcacatcgc
    10381 cggagtgggc ttcgacctgt acgtccggct cgtcggtgag gccgtcgcca gcttccgcgg
    10441 tgagagcgac gagaccccga ccgagatcaa gatcgagctg cccgtcgacg cgcacctgcc
    10501 gcacgactac gtgccgcacg agcggttgcg cctggagatg tacaagaagc tcgccgccgt
    10561 cgtcgacgcg gccgagctcg ccgagatcga ggccgagctc gtcgaccgct acggcacacc
    10621 gccccaggcc gtggtcaacc tgctcgaggt ggcccggctg cgcaccgtgg cccgggccgc
    10681 gggcatcgcc gacatcagcg tgcagggcaa ccacgtgcgc ttcggcccgg tcgagctccg
    10741 cgagagccag gaactgcgcc tcatgcgcct gcaccccggg tcgatcatca agcccgcgct
    10801 cggcaccatc ctcgtcccga agccgatgac ggcccgcgtc ggcgggcgtc cggtgcgtga
    10861 tcgcgaggtg ttgtcgtggg ctggtgaggt catctcgtcg gtcgtcatgg acaatgtggc
    10921 tcaggccgcc gcggtggccg cctcccgtgc gtagcggggc ccgctctcga aaggacagct
    10981 cgtgactctc tccgttcgcc gctccctcgc ggtgctcgcg ctcggcgccg cgctcgtgag
    11041 cgccggctgc tcggtcaacc gggccgagac cgcggccgtc gtcgacggga acgtcatcac
    11101 cgagggccag ctccaggtcg ccatggccca gatcaactcc atggagccgg cgctgctcca
    11161 ggagcccctc acgccgagcg gtgcactgac cgcgctcgtg caggcgccgg tggttctcga
    11221 cgtcctcggg gacaagggca tcgtcgtctc cgacaccctc gcgacccgtg aggcgcaggg
    11281 gcgtggggtg gccaacccga gtggcgagac gcttcaggtg atccgcttgg cctccgccat
    11341 caccgccgcc cagcagtccg gtcagatcac cgaggccgac ggcgcggcga tcaccgaggc
    11401 gatgggggcc ctcgacgtcg aggtcaaccc gcggtacggg accttcgcgc cggagaccgc
    11461 gtccatccag ctgacggccc cggagtggat caaggccgag gaaatccccg cgccgtgagc
    11521 gctgtgcccg gcgcgcccag cgggcgcctc gggatgctcg tcacctctcc ccgggtggct
    11581 ccgggtctgc tctcgcacgg tgcctggcag gtcgtccagg gctatgcggt tcggctggcc
    11641 cgctcgttcg acgagccggt ggcccaggcc gtcgaggagg ccgggttcac cgtcgagcag
    11701 gtcggtgacg cgtcgccgcc cgagctggcg cggcgtctgg tggacctcgc cggtgccgtg
    11761 tcggtcgtgt ggctcgggag tgctgacggt gacccgggtc tggcggatgc cgtggcggcc
    11821 gaggtctcac ggctcgcgac cccgcccgag gtcgaactcg tcgtcgggtc gtgggacgtc
    11881 cagggcgggc gcctgctcga cgtcgtcgcc accatggacc ggttgcgctc acccggcggc
    11941 tgcccctggg acgccgagca gacccacgag agcctggcgc cctacctcgt ggaggaggcc
    12001 cacgaggtgc tcgacgccat cgctggcggc gaccgcggtc acctcgcgga ggagctcggc
    12061 gacatcctgc tccaggtggt cttccacgcc cgggtcgccg aggaggcggg ggagagcgcg
    12121 ttcgacatcg acaccgtggc cggcctgctc gtcgacaagc tcgtccgccg gcatccccac
    12181 gtcttcgccg acggggatgc gtccaccccg gccgaggtcg aggccgcctg ggagagcatc
    12241 aaggccgtcg agaaggcggg ggtcggggga gatgactacc gcgccgacct gctgcacggc
    12301 atcccgacgt cgttgcccgg ggggctcgcg gcggacaagg tgctcgcccg ggtccgccgc
    12361 cgcggattga cccccgaccc ggatgccgtg gcgcgcgtcg agcgtgcccg cgcggctctc
    12421 gccgcggctg agtccgaggt gcgggctgcc ctggaggggc tgcagcgctg aggctgccgc
    12481 cggcccgcgc gaggtcccgg ctgtgctgat cgagtgagcg caacatgccg tcggcgttca
    12541 ccgaacacgg tgtgttgcgc tcactcgatg gtcagcgttg cggggtcgat gtccattgcc
    12601 cgggcgaaac gacgcagcaa cgtgcagcgc cgggctcgtc cgtagacgtc ctcggcgtag
    12661 atctcgatca cctggtagtc ctcgtccgtg gcaaggcccg agcgcatgct gtccgcgctg
    12721 cggcgcttgc gcgatgcgtg atcagcaccc tggtactcac cgatgactcg ctgacggcgc
    12781 cagaagaagt ccccgtcgag cagccacccg ccgtggcagt cgaggacctc ggcgttgagc
    12841 tcgggttcgg ggaatcccgc tcgatggaac atcagccggg tccgggtctc catcggcgac
    12901 cgcgagccca ccctgatcag gccccccgct gccatgagtc gcgcacgacc tcgtgggcgt
    12961 gtcctcgagt cgagggctga ccggagcgtg gcgattccct cgggctcgag tcggttgacc
    13021 accgcgtcgc ccatgaccac gaggtcatcg atcgccagcg ctggtgaggt cagttcgccc
    13081 atgtcgcacc aggtgtctgc gggagcgacc acgcgcaggc cctgcacgtc gatcacctcg
    13141 cgtcgttcaa gccctcgatg gccgcggcat ccatcgcgac gaacttgtgc ccgggacgtg
    13201 ggccgcatga catcgagcac ctccggggcc tccagcgccg tgggtagggg caggcccagc
    13261 agctgggcgg cgctgacgtg ggagaaggcg gcatcgtccg gaaggccgag cgcgaaggcc
    13321 gctgcctttt cctgcaccgt ggccggtgtc gagatcatgc gcacgccgtg tgtcgggacg
    13381 tgcagccggg tcgagcgcag ggtgcgcagc cgcagtccgg cctcccgacc ctgggcaacg
    13441 gtgaaggggc gggaggtcaa ctccgacggc agccccagcc cctccatggc cccaccctgc
    13501 atggtgcgtc tcgccagccc gggaacttat ccacaggggc ccgatcgggc ccagaagatc
    13561 caaagtcgag tgagcgcaac acgccgtcag cgttcaccga acacggcatg tcgcgctcac
    13621 tcgatggccg gctacctcga gcagggccac cccttgcttg tagacccaag gttcctttag
    13681 aattccaatg tgaaactgac tgctcgcgaa cacgagatcg tcaccctgct gcgtgccgac
    13741 cccatgctcg acgccgcagc cctggccgag cgcatcggct ccacccgggc cgcggtctcg
    13801 gtgcacctgt ccaacctcat gaagaagggg gtcgtccgcg gtcgcggcta cctgctgcgc
    13861 cccgaggccg acaccgtgct cgtcgtcggc ggcgcagtcc tcgacaccaa ggtgcgcacc
    13921 gctggccccc cggtcctcgg cacgagcaac ccgggggcgt cgtcctccac ggtcggcggg
    13981 gtgggccgca acatcgcgga gaacctcgcc cgcctcggcc ggcccaccgt gctcgtcgcc
    14041 cccgtcggcg acgacccggc cggtgaggcc gtcgccgatc gcaccaccgc cgccggtgtc
    14101 gactgcggcc acctcgtgcg ctctccccac cccaccggca cctacgtcgc ggtgctcgcc
    14161 gacagcgggg acctgcacat cgcggtggcc gacatgcgcg ccgccgacga gctcgccgtc
    14221 gccgacctcg cgtccgttcc gggcctgctc accggtgccg acgcactcgt cgtcgacgcc
    14281 aacctgtccg agtccgtcgt gcggtggctg ctcaacgcgg ccgccgaggc gggggtgctc
    14341 agcatcctcg acccggtgag cgtcgccaag gccacggcgg tcgcacccgt gctcgacggc
    14401 acgatccccg tgcacaccgt cacgcccaac gtcgacgagc tctccgccct cgtcggtgct
    14461 cccgtcgagc ccgacgtgga tgccgtgcga gcggcggcag ccctgctcca cgagcgcggg
    14521 gtgagccacg tgtgggtccg ccgcggaacc gccggcagcc tgctctcgat cgccacggca
    14581 tccacccccc gcgtcgtcct cgtcgatgcc ccgccgaccg acgtcgccga cgtcaccggt
    14641 gcgggcgact cgatgaccgc cggcttcgtc aacgccctgc tcgacggcgc cgacgtcgtc
    14701 gaggcagccc gccacggcca ggtgctcgcc gcgctcacct gcgcctcgcc cgacaccgtg
    14761 cgcgacgacc tcactcccgc gctcgtcgcg gcccacctga ccccgtccca gccgtccacc
    14821 aaggagctcc accgatgacc accagcacgt cgaccacccc gcaccccctg ctcaagctcg
    14881 acccggaggt cgcgcaggcg ctcgccgacg gcacccccgt cgtcgcgctg gagtcgacga
    14941 tcatcagcca cgggatgccg tacccggcca acgtcgcgat ggcgcgcgag gtcgagcaga
    15001 tcgttcgcga cggtggagcc gtgcccgcca cgatcgccgt gctgaacggc accccgaccg
    15061 tgggcctgtc caccgacgag ctcgagatgc tcggctcgcg cgacgacatc cgcaaggtca
    15121 gcgtgcgcga cctgccctac gtcgtggcca ccggaaccca tggcgcgacc acggtggcga
    15181 gcacgatgcg gatcgcgggg ctcgccggca tccgggtctt cgtcaccggt gggctcgggg
    15241 gtgtgcacca gggggccgag agctcgatgg acgtgtccgc cgacctcacc gagctgtcac
    15301 gcaccgaggt cgcggtgatc tcggccgggg tcaagtcgat cctcgacatc ggccgcaccc
    15361 tcgaggtgct cgagaccctc ggcgtgcccg tcgtcggcta cggcacggac gacttcccct
    15421 cgttcttctc ccgcacgagc ggcctgcgaa cgcccatgcg cctggacacc ccgcaggaga
    15481 tcgccgccct catggaggcc acctggggcc tcggcctcgg cagtggcatc tcgatcgcca
    15541 acccggtgcc cgccgagagc gagatggcgc acgaggacat cgactccgtc atcgcccggg
    15601 ccctggccga cgccgacgcg ctgggcgtga ccggcaagga catcacgccc tacctcctgg
    15661 gccggctcgt cgagctgtcc gatggtcgtt ccctggagac caacctcgcc ctcgtgcgca
    15721 acaacgccgt ggtcggcacc gccatcgccg tggcctacgc ggcacgctga ccgcccgggc
    15781 ccggggacgg gtctgcccac gaagcgggtc gcgcccagcg caggtcggtg acgaggacta
    15841 ggctcggacc cgatcccatc cagaacaact ttcaggagtc cggcgtggcc agcatcgagg
    15901 cagtaggcgc ccgcgagatc ctcgactcgc gcggcaaccc cacggttgag gtcgaggttg
    15961 ccctcgacga cggcaccctc gcgagggcgg ctgttcccag cggcgcatcg acgggggcct
    16021 tcgaggcctc cgagcgccgt gacggggaca aggaccgcta cctcggcaag ggtgtgctca
    16081 aggccgtcga cgccgtcatc gacgacctcg gccccaagct gctcggcttc gaggccagcg
    16141 agcagcgcct catcgacgcc gagatgctcg ccgccgacgg cagcgccaac aaggagaagc
    16201 tcggcgccaa cgccatcctc ggtgtctcgc tggccgttgc ccgcgcggcc gccgactccg
    16261 ccgacctgcc gctgttccgc tacgtcggtg ggccgaacgc ccacgtcctg cccgtgccga
    16321 tgatgaacat cctcaacggt ggcagccacg ccgactccaa cgtcgacatc caggagttca
    16381 tgatcgcgcc gatcggcgcc gacagcttcc gtgaggcgct gcgctggggg gccgaggtct
    16441 accacgcgct caagggcgtg ctcaaggagc agggcctggc cacgggcctc ggcgacgagg
    16501 gtggcttcgc gcccaacctg ccgtccaacc gggccgcgct cgacctcatc ctcgtcgcca
    16561 tcgagaaggc cggctacacc ccgggcgagc agatcgccct ggcgctcgac gtcgccgcct
    16621 cggagttcca cgacggtggt gtctactcct tcgaaggcgg ctcgaagacc tcggccgaga
    16681 tgatcgacta ctacgccgag ctcgttcgtg actacccgct cgtgtccatc gaggacccgc
    16741 tggacgagtc cgactgggac ggctggaagg ccatgaccga ccgcatgggc aacgacgtcc
    16801 agctcgtcgg cgacgacctg ttcgtcacca accccgagcg cctggcccgc ggcatcgaga
    16861 ccggcaccgc caacgccctg ctcgtcaagg tcaaccagat cggctcgctc acggagacgt
    16921 tggatgccgt gtcgctggcc cagcgcaacg ggtaccgctg catgatgagc caccgctccg
    16981 gcgagaccga ggacacgacg atcgccgacc tcgccgtcgc caccaactgc ggccagatca
    17041 agaccggcgc accggcccgt tccgagcgcg tcgccaagta caaccagctc ctgcgcatcg
    17101 aggaagagct ggacgacgcc gcggtctacg ccggcgccga ggccttcccg cgctttgccc
    17161 gccgccagca gggctgagta ctcgtatggc caccgggcgc tcccgcggtc cggcgtcgcg
    17221 accaccgcga cgtccggccg gggggcgccc gtcggcgtcc acccgtccag cgggctcgtc
    17281 ggcgcctccc gcggcggtgc ccgtcacggg gtggcgtcgg ctgctcggcg gggtcacggg
    17341 tgaccgccgg gcccgtcgtg cactggccct gaccgccgtg ctgggcctgc tcgtcatcct
    17401 gctcgggtcg acggtcaccg cctacatcgg gcagcgcggc gacatcgcgg cgctgcgcga
    17461 caaggttgcc gcccaggagc aggacgtcgc ggcgctccag gccgagcgcg agcgctggcg
    17521 tgaccccgcc tacgtcgagc agcaggcccg gcaacggctg aagttcgtca agcccggcga
    17581 gaagtcgtac acggtgctcg acccggagcc cgacgccggc aaccgtgacc cgttggccgc
    17641 cattgcctcg tcggacgagg agctgccctg gtaccagggt gtctgggagt ccgcacgcac
    17701 cgcggacgcg ccgggagccc gccgatgagc gcaggccacg acctgccgcc cgtcgatccg
    17761 gccgacctgg aagccgtgac ccggcagctc gggcgcgagg tccgtggcgt cgccgaggtc
    17821 gctcaccggt gcccctgtgg cgagccggat gtcgtgcgca ccgagccgcg actgcccgac
    17881 ggcacgccgt tccccacgtc gttctacgcc acctgcccga agctgaccgg tgccctgtcg
    17941 accctggaga gccagggggt catgcgcgag atgtcggagc ggctcgagca cgacgaggcc
    18001 ctgcgcgcgg cgtatgccgc ggcccatgtc gactacctcg agcggcgggc ccagctcggt
    18061 gacgtgcctg aggtcgccgg catctccgcc ggtgggatgc cgacccgggt caagtgcctg
    18121 cacgtcctgg tcgcccactc gctcggggcg gggccgggcg tcaacccgct gggggacgaa
    18181 gcgctggcga tgctcccgca gtggtggcaa cggcatccgt gttcggccga cgcacccggg
    18241 tctgccgcgg cccaggggga ggcctcggcg tgagcggggc cccgactcgg gtcgcgggga
    18301 tcgactgcgg gacgaactcg atcaggttgc tcatcgccga cgtggatgcc ggtggggcgc
    18361 tcgttgatcg ggtgcgccgg atggaggtcg tgcggctcgg gcagggcatc gatcgcacgg
    18421 gggtgatcgc gcccgacgcg atggagcgga ccttggcgat gacccgcgag tacgccgcgt
    18481 tgtgccgtga gcacggggtc acggcagtgc gcttcgtcgc gacgtcggcc tcgcgggacg
    18541 cgcgcaacgc aggggtgttc gtcgaggggg tgcgggccgc gttcggtgat ctcgatgtcg
    18601 tgccggaggt ggtggagggc atcgtggagg ccgagttgtc gttcaccggc gcgaccggtg
    18661 gccttcgggc cgctgccgtg ccggggccct atgtggtcgt cgacctcggg ggcggctcga
    18721 ccgagctcgt gcgagggacg gcccacgtcg aggcggcgta ctcgatggac atcggcagcg
    18781 tgcggatgac cgagcgtcac ctgcacgacg acccgccgac ggctgatcag gtggcggccg
    18841 cccgggccga cgtgcgtgcc gcgctcgacg tcgctgaacg cgaggtgcgc ctcgacgggg
    18901 tcggggccgt cgtcgggctg gccgggagca tcacgacgat caccgcgcac gcgctcggtc
    18961 tgacgaggta cgacccggag gcctcccacc tggctcgcat cccggcggac gtgcaccgcg
    19021 aggcgtgccg ctcgttgctc gcgatgagca gggagcagcg ggcggggctc ggcttcatgc
    19081 accctgggcg ggtcgacgtc atcgggggag gggcactcgt gtggctcgag gtgctcgacc
    19141 gggtgctggc gcgctcgccc gaggcggtcg tcgtgacctc ggagcacgac atcctcgacg
    19201 gggtcaccct gtcgctggcc tgaggctggg cgcttcgccc agccggtgca gatctgacca
    19261 ataggtcaga tcgtgtacag cgacgcgccg gtgctatttc gtggtgcgca ctaatacgca
    19321 atccgcggta gtgtgcgaac tgcatcatcg gggtgcgcgc gaaggagggg tgccatggag
    19381 ggcacacagc tggtcaaggg ggtgctcgac ctcgccgtgc tcgcggtcgt cgcccgggag
    19441 gacggctacg gatacgacgt cgttcgccgg ttgcgctcgg cagggctcga ggaggtgggt
    19501 gacgcctccg tctacggcac gctgcgccgc ctctactcgg ctggcgcgct gacctcgtac
    19561 gtcgtgccct ccgacgaggg cccgcaccgc aagtactacg gcatcaacgc ccagggccgg
    19621 cacctgctgg cgaccgaggg caagacctgg gcggcgttcg ccgcgacgat ggacggtttg
    19681 ctcgagcagg caggagtgca gtcatgaacg acgcggaagc catgggcgcg acaacccaag
    19741 ggggctcggc ctcagcaact caggtgctgc ccaccggtgc cgagtcggcg caggctcgct
    19801 cggccgacgt gcgcgcgtac gtcaccgcgg tgcgcagttg gctctcggac ctgcccagcg
    19861 acgaggtcga ggacctcact gccggcatgg aggctgacct cgacgagcgc gcggccgaaa
    19921 ccggagatcg gctcggggcg ctgctcgggg agcccgaggc gtatgccgca gagttgcgcg
    19981 ccgcagccgg cctcgccccc cgggtggtgc cggtcgtcgg tgcgggcgcc gcgggtccgg
    20041 gtctcgtcga ggccctggtg acgctgctac gggccagggc cgacggcatc ctcacccggt
    20101 ggccgtggct gcgtgagctc cgcccgacgt ggtggctggc ccggggagct gcgctcggct
    20161 ggttgctgtc catgtggact ggtaccggtc gtacggttct cctgccgttg ctcggcgcgg
    20221 tcctctcatt ctggttgggc ctcacgatgc ggcgccgtga cccactgcgc ccggcagcgt
    20281 ggacatcact cgcggtcgct aacgtcatcg ccgccctggc ggtgttgccg atggtgtcca
    20341 cgtacacgca gagctacctc gggtcggaca acgagtacct gcgcgagggc tatgcgccct
    20401 acccagaatc gatcaccgcg aacggcgagg aggtgcgcaa cctcttcgcg tacgacgcgt
    20461 caggcaaccg cctcgacggg gtgcgtctgt tcgaccaggc cggtcggccg gtcgtcatcg
    20521 acacgaacct gctgttcgac gggtccttcg aggacatccc gacccggcca gacgacggga
    20581 tgccggacgt ggcccatgac gtcttcccgc tgcggtgggc gggccacgac gcctgggctg
    20641 atccccggat gccgtggacg ccgccgctcg ttctcgcacc ggttcccggt gtgacgccgt
    20701 ccacggcggt cagcccgacg ccgtcaccga cctcgactgc ctcgccgagc gccagcccga
    20761 gcgcgacctc gacagagagc ggaacgccca gcccttcacc gaccgtgaca actccctgac
    20821 tccgtgcgac ttcggtgcgc catcgggccc ggaacgggcc gatgagcgca ccgaagtcgc
    20881 acgaagtcgg aatgggggat ggggctgggg atggcggcta cagtggcgtc gcccccgtgg
    20941 cccaactggc agaggcaggc gacttaaaat cgccacaggt gcgggttcga cccccgtcgg
    21001 gggcaccagc actgtcatcg ccgcagcccg gttgtccaca ggcgtgtggc gagggaacac
    21061 gaggccactc ccgcacgctt atcctgatga cacgtgcggt gactgccacc gccatgacca
    21121 tcacggagga acgatggcca gcaatccggc cttcaatcgc atcgaaaagg atgcccagag
    21181 cggctacgcc ggcttcgggg gccagggtta ctcatccccg tcacccatgt cggcccagcc
    21241 gaacatggcg gagatgactc cccagcaact tcaggacatg tacaacgggc cgtcggccgg
    21301 cccggtcgac acccggcgcg tcaccatgga cgacgtcatc atgaagacgc tcggcctctt
    21361 cgccatcgtc ctcgtgaccg gcgccgtcgg ctggaccgtc gctgcggcga accccggcct
    21421 ggggatgatg ctgtggctgg gcggcatgat cggcacgctc gtgctcggtc tcgtcatcgc
    21481 cttcaagaag gccctcagcg ttccgctgat cctggcgtac gccgtggtcg agggtctgtt
    21541 catgggggct gtgagccagt acttcaatga ggcctacccc ggcatcgtcg gccaggccgt
    21601 cctcgcgacc ctgtcgacct tcgccggcat gttcctggcc tacaagttcg gcctgatcaa
    21661 ggtgacggcc aagttccgcc gcatcatgac gatgatgatc ttcggctacg cgatcttcgc
    21721 cgtcgtgaac ttcatcttcg cgatggtcac gcccacggcg tttggtttcg gtggctccgg
    21781 tcccctcggc atcggcatct cgctgttcgc cgtcggcctg gcctccttca ccctcgccct
    21841 ggacttcgac tccatcgagc aggccatccg caccggcgca ccggcgaagt actcgtggct
    21901 cctggcccac gggctgatcg tcaccctggt gtggctctac atcgagttcc tgcgcctgtt
    21961 cgctcgcctg cgcgagtgac cgagcacgac gcgcacgcac ccgccggggc aggttcacct
    22021 gccccggcgg tgccgtctgt gcacccgggc ggcaccttcg gcgacccggg agcggatgcc
    22081 gggcggccgg catccgtcga cgtcgtcgcg tcgcaggccc gcaccgagct cgaccgcatc
    22141 cgccggcggt gggccgagct tccgatcgcg gcagcggagc agcggatgcc ggtgctccgc
    22201 caccttctcg ccgacctcgc cccccgcacc ttcccgcgac ttcttgcact ccagccacct
    22261 gagcgcccac cgccggaggt ggctggagcg caagaagtcg cggaggtgcc ggacctcggg
    22321 ccggccgtcg tggtcgacca gctcagcgtg ctcgtctggg acgcctacgc gcagggcaac
    22381 ggcgacggca tccccgacct gctgaccgca gcccggcgcg ccctcgcctg acgcgccctc
    22441 gcctgacgcg cgatgagtgc ggaagtggtt gccatgacag ccacttccgc actcacggcg
    22501 aggagatacg gggtcagctc agtcgctcga cgatgatcgc catgccctgg cccccgccga
    22561 cgcacatcgt ctccaggccg aactggccgt cgcgcgtctg caacccgttg atgagcgtgg
    22621 tcatgatgcg cgtccccgtc gacccgaacg ggtgacccag tgcgatcgcg ccgccgtgca
    22681 cgttgagccg ctcggggtcc atgcccaggt cgtcggcgct gggcagcacc tgcgccgcga
    22741 acgcctcgtt gatctcgtag aggtccatgt caccgatgct catgcccgcc cgggccagcg
    22801 cctgacgcga cgcctcgacc ggtccgaggc ccatgatctc cggcgagagc cccgacaccc
    22861 cggtcgacac gatccgcgcg agcggcgtca gccccagctc acgcgcccgg acgtcgctca
    22921 tcacgacaac cccggaggcc ccgtcgttca gcgggcagca gttgcccgcc gtgacggtgc
    22981 cctcctcgcg gaacaccggc ttcagccccg acacggcctc cagcgtcacc ccggcccgcg
    23041 gcccgtcgtc ggtcgagacc accgtgccgt cgggcatcgt caccggcagg atctcgcggg
    23101 cgaagaaccc gtcggcgatc gcctgctcgg cccggttctg gctcagcaca ccccactcgt
    23161 cctggcgtgc ccgcgacacc ccgcgcgcgg tcgcgacgtt ctccgcagtc tgccccatcg
    23221 agaggtagat gtcgggcagc atcccggcct cacgcgggtc ggtccacgtc gcgttggacg
    23281 ccgccatctc ggccgagcgg gcagcagaat cggcgaacag ggggttctgg gtgtcggcgt
    23341 cgctgccacc cgcacccgcg aacgaggtgt actgcgagac gcactcgacc ccgccgacca
    23401 agaacgtgtc gccctcgccg gccttgatcg cgtggaaggc catccgcatc gtctgggtcg
    23461 agctcgcgca gaagcggttg atcgtcgcgg ccggcaggtg gtccatgccc gcgagcaccg
    23521 cgatgacccg cgccatgttc gacccgtgcc gtccactcgg gtcggcgcag ccccagtaga
    23581 ggtcgtcgat ggtgctcggg tcgagacccg gaagcttcgc cagcaaaccg tgcaccacct
    23641 gtgccgacag gtcgtcgggg cggatgtcct tcagtgaacc cttgaacgcc cggccgatgg
    23701 gggttcgggc ggtggcgacg atgacggcct cggtcacgag ggctccttgg gggtgggacg
    23761 cgcaggcgtc gggacgggag acgcgcgggg gcgtccggcg aacggcatcc actaagcgct
    23821 tgctcagtgg atgccgcaag ggtagccggg aggttcccgc ccgaggtgtc cccgtggtca
    23881 ccatcacgcg gcgaacggct gccggcaggg ccatgatgca ggtatggaca ccacgcgnnn
    23941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac gtcgtcgcgg cgatctcctc
    24061 cgcccacggt gagatcttcg gcgacatccg cccggccagc acgatggtcg tcgtcgccgg
    24121 gctgctcgac ccacgctgga aggtcgagat cgaactcgac gccatcgtca cggactgagc
    24181 tgacccaggg tcccggcgtc aggcgctgcc gggactcgcc ggggagtccg aggttggtgc
    24241 accgaggtcg gatgccgatg gctcgccctc ggccaccgcg tcactgccgt cggtggtgac
    24301 gctcgcctcg atcgggctct cctcgaccgg gtcctgcggt ggtgggacgg cgtcgcggcg
    24361 tcggcgcagc agcaccgccc agcggcctcg gggtccgcgc gactgcccgg cgatctcggt
    24421 gggggcgact tcggtgcccg gctccttgac ggcctggccg gcggccacgg cgacgggccc
    24481 gacaccctcc ccgcgccgcc tttcgggtgc ccggtccacg gtgtcgacgc cccacacgcc
    24541 cagggcagcg cacaccgtcg gcagcatcgc cgaggcgacc cgggcgtagc ccgcggccga
    24601 cggatggaag cggtccttgc tgaacatctc gtgaggggat cgctcgaact ccgggccgag
    24661 caggtcggcg agggagaccg tgcggccgcc ggcctcgacg accgcgacgg tctgcgcggc
    24721 ggcgaggtct cgcgaccagt gcttcatcag cgcccgaagt ggctgggcga tcggctggac
    24781 ggtgccgagg tcgggacagg tgcccacgac gacctcgctg cccagggcgc ggatgcgccg
    24841 aacggtctgc tccagcgccc gcaccgaggc cgagcgctcg atgcgatggg tcacgtcgtt
    24901 ggcgccgatc atgatgacga cgacgtccgg ccgcagcacc tcctcgaggg cgttggccag
    24961 ctgccgctcg aggtcgctgg actcggctcc gacgacggag cggttcgtca gctgcacccg
    25021 gcgcccggtg agggcggcga cgccgttggc gatgatcgcg ccgacggtct cgtgcggggc
    25081 gtctgcgccc aggcctgctg ccgagctgtc cccgagcacg acgatatcga aggggcggcc
    25141 cgcgccggag ccgtacacgc cgttgtcatc gggggaggag tcgaaggggc tgccgatgac
    25201 ccggcgggcc agcgcggcct cggccttgat cagcccccag cctgccgcgc cgagggcggc
    25261 gcctgccgcg gcgatcccac cgccgccgta ggccgcggtg gcggcgatct tgcgggctct
    25321 gcgtgctcgt cccatgcgac gtcaccccct ctctggtcct cggtgctgag cgtctgacgc
    25381 tcagcgcttg cgggcctgca gcgtataggt gagcggcatc cgctcggggt ggttcgtgag
    25441 acgccactcg tcgtgggggc catcgcgcac catccgcccg ggcagggcct cccacggcac
    25501 gctgcggtgc tcgacgaagc cggtgacctc gaaccctcgt cgcagcaggg cggtcatggt
    25561 ctcgccgatg ccgtggttcc aggacagctg cttggtgttg acgaaccggt ggtcgctctc
    25621 gacataggtt ccctcctcct cgatgtcgaa gggctcgggc gt
//
LOCUS       DAOSOC010000017        25553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1929_length_25553_cov_8.968900, whole genome shotgun sequence.
ACCESSION   DAOSOC010000017 DAOSOC010000000
VERSION     DAOSOC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 25553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25553
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1929_length_25553_cov_8.968900"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="pdxS"
                     /locus_tag="PL014_02640"
     CDS             complement(<1..752)
                     /gene="pdxS"
                     /locus_tag="PL014_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOT56023.1"
                     /translation="MTEQQSAPGTGTTRVKRGMAEMLKGGVIMDVVNAEQAKIAEDAG
                     AVAVMALERVPADIRAQGGVSRMSDPDMIDGIIAAVSIPVMAKARIGHFVEAQVLQSL
                     GVDYIDESEVLTPADYANHIDKWAFTVPFVCGATNLGEALRRITEGAAMIRSKGEAGT
                     GDVSNATTHMRQIRQEIRRLQNLPEDELYVAAKELQAPYELVKEVAAAGKLPVVLFTA
                     GGIATPADAAMMMQLGAEGVFVGSGIFKSGNPA"
     gene            complement(817..1404)
                     /locus_tag="PL014_02645"
     CDS             complement(817..1404)
                     /locus_tag="PL014_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56024.1"
                     /translation="MNDTLQIATFVVIVLLVAAWYLSYSAARLDRLHAKVEGAMSALD
                     AQLIRRAEAALELANSGVLDPASALLIADAATESLERTTEQPVTDDLLDGQHFGGREH
                     VESDLTAALAAALPGEVVVELRAAGDEFVIDELDRVAASVLRVQLARRFHNDAVRDVR
                     RVRAKRTVRWFGLAGHAALPELVVFDDDVPLALPA"
     gene            complement(1404..2612)
                     /locus_tag="PL014_02650"
     CDS             complement(1404..2612)
                     /locus_tag="PL014_02650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT56025.1"
                     /translation="MRIGMVCPYAWDVPGGVQYHVRDIAEHFIRQGHEVWVLAPAESD
                     ADLPDYVTSVGRPVPVRYNGSVARVTLGPATSTRVSRWLEAGNFDVLHLHEPLNPSVS
                     LVALWASTEPVVATFHSAMIRSRALHAAHPLLRPSLEKIRGRIAVSEDALGTVQRHLG
                     GMAFIIPNGVHVDSFALAPVRPEWAGTSEAPTLAFLGRVDEPRKGLAIALQAMPALLE
                     VHPGIRLLVAGPGDASDLLKDADPRVAAATVMLGMVTDDDKASLLRSVDAYVAPHLGG
                     ESFGIVLVEAMAAGAPVVASDLPAFAAVLDVGATPADGDANGILFETGSPQGLAQALL
                     ALLADPARREALRVAGRARSRVFDWDVVAAKVMAVYETVIAGADAEPDEPAPQGLWGR
                     LVRGVPGGGN"
     gene            complement(2609..3550)
                     /locus_tag="PL014_02655"
     CDS             complement(2609..3550)
                     /locus_tag="PL014_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HOT56026.1"
                     /translation="MRLLSASAADRLTLAGHQLGWRAVRRMPERLAYALFDRVADVAV
                     ARGSAARMRSNYARVRPELDDAALDALVRDGMRRYLRYYCEAFRLQDLGVGDLAARVR
                     VEGDGPVREVIDAGGSVVAFLGHLGNWDLAGAWATSHLGAVTTVAERLKPEEVFEEFL
                     AFRESLGMTIIPLTGGVNPFPALREASRRPGIIPLLADRDLTDRGVEVDLCGHRARMA
                     PGAAALALAERRPLYPVTIRHERRGSGWGIVITFHQQVPVPETGTTRERATAMTQACA
                     DALGTVITQHTSDWHMMQRVFIDDLDPARLRAAHGAA"
     gene            complement(3547..4173)
                     /locus_tag="PL014_02660"
     CDS             complement(3547..4173)
                     /locus_tag="PL014_02660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT56027.1"
                     /translation="MLNRYARAFFTKLLSPVAAVLVRLGISPDVVTVIGTLGVCFGAL
                     AFYPRGELLVGTLVITFFVFSDTLDGIMARSTGRSGNWGAYLDSTLDRVGDAAIFGGL
                     VLYFAGAGRQEGELAPVMAGLALACLILGSVVSYAKARAEGLGMTANVGIAERADRLV
                     VVLIATGFTGLLDLPWIYLGVVLALLALASLITVVQRMLEVRRQALAL"
     gene            4268..6391
                     /locus_tag="PL014_02665"
     CDS             4268..6391
                     /locus_tag="PL014_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT56028.1"
                     /translation="MNGTATKAPTVTTTTSIRNVVLVGPSGSGKSRLFDHIVGSAVPA
                     RAPRSDGGDAGDATTGLRAATIATGSVVVTLLDAPGNPDFVGEVRAGLRAADAALFVV
                     SAADGADAASRALWHECAVVGMPRAVAITALDARDADFTATLADCQAHFGAGIQPLGV
                     PVAGDAGAVTSIADLLLGEIHDYSAGERTVRPAGPEHAEVFDTFRPSLIEGIIEESED
                     GTLMDRYLEGEDLDFATLEKDLLTAIAHGTFHPVLPLSAETGAGVGVLMHLIEAAFPH
                     PALHPLPTVTPVAGGTAVQLTADPDGPLVAEVVHTESDAYVGHRSLVRVFSGTLRGDR
                     PVHVSGHLERLGVPTGEGHAPHDDDVRPGALAAPLDDELLPKTEAIAGEIVVVTKLGS
                     AQTTDTLSSPDQPLLVTPWALPEALLPSAVAPSSRADEDRMPGAFRELAAEDPSLRIE
                     HDAATGQVVLWTTGPAHLDLVLARLRTRFNVSVDQVPVQVALKETATARAEALGRHVK
                     QSGGHGQFAVCHLVMEPLPRGSGIEFAEVVVGGAVPRQFVGSVEKGVHTQLEKGLLAG
                     WPVVDVRVTLTDGKAHSVDSSDMAFQTAAGLALRQLASPSTMTLLEPIDTVSVSVDDE
                     HLGAVMTDISTRRGQILGSAPAVDQPGRSVLEAAVPQLELLDYAVSLRSLAHGTGTFH
                     REHRGYEEMPERLAAKHLGERGRHA"
     gene            complement(6396..6680)
                     /locus_tag="PL014_02670"
     CDS             complement(6396..6680)
                     /locus_tag="PL014_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOT56029.1"
                     /translation="MAKVEHDLTDEQWSALQVAWAGCAYCGATDRPLQRDCVLPISRG
                     GRYTVRNVVPACGPCNASKCNDEVTGWLRRKRLDERAFLTRLVEIGASLE"
     gene            complement(6725..7267)
                     /locus_tag="PL014_02675"
     CDS             complement(6725..7267)
                     /locus_tag="PL014_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOT56030.1"
                     /translation="MPTHEDAADFAGEGDGFERLWTPHRMVYIQADRPDAEPGDGCPF
                     CAAPGKDDAAGLIVHRGEHCYVVMNLFPYNPGHVLICPYRHVPLYVDLTDEETLEFTA
                     LTKATIRALEAASAPMGFNLGMNQGAVAGAGVAAHLHQHVVPRWGGDANFLPIIARTK
                     ALPALLEDVRSRLVEHWPTP"
     gene            complement(7392..8225)
                     /locus_tag="PL014_02680"
     CDS             complement(7392..8225)
                     /locus_tag="PL014_02680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT56031.1"
                     /translation="MSESTSAAGPSRGILVTGASRGVGAAVARAFAERGDRVVVHHRG
                     PSSLDRAAAVLEGLPGSGHVRLAADLSDPDAVARLASEAATALGRVDVLVNNAAMIVP
                     PAPGVDGSRRGDHPLELTSYEDWVQIWQRTLATNLLGPANLTWCVARQMIDVPPADGV
                     PVGRVVNVGSRGAYRGEPDIPAYGASKAGLHSFGQSMAQRLAPHGIAVTSIAPGFIET
                     EMVGAALEGAAGEATRAQSPFHRVATPQEVAAAVVTLASPGAEWASGAVLDFNGASHL
                     R"
     gene            complement(8236..8928)
                     /locus_tag="PL014_02685"
     CDS             complement(8236..8928)
                     /locus_tag="PL014_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT56032.1"
                     /translation="MAIVDAALGVLTRRDHHHVERIPASGAVVVVSNHLSVSDPLVLA
                     SALRRAGRQGTFLVMAEAFAWPGVGWLLRRTGQISVRRDRDPAAALQPALDALGRGWA
                     VAIYPEGKITTEPDYRPMSEVRTGAVRLALTANCPIVPVAQWGAHRLLTREGTSSLVR
                     PLRWLGRLRPGRLSRRPTVVVAVGEAITPAQLRARVADPEDLRAMTAVIMDDLRALLA
                     EIAAEDLPDLRG"
     gene            complement(8946..9434)
                     /locus_tag="PL014_02690"
     CDS             complement(8946..9434)
                     /locus_tag="PL014_02690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT56033.1"
                     /translation="MDGDAAQTSGWRVREARPVDAFAIAALHIQHERELGHAVPVGFL
                     DEFAQAWLRDTARRTWLAEDPRGRPLGVLHGTRVQRLPSAHRPADAWFHVSLLFVSIG
                     VRGAGIGEGLLDAMLAWAEGDGVSRVQLHALPGVRTIFERFGFGPPSQQLMERQISRR
                     GG"
     gene            complement(9811..11796)
                     /gene="thrS"
                     /locus_tag="PL014_02695"
     CDS             complement(9811..11796)
                     /gene="thrS"
                     /locus_tag="PL014_02695"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT56034.1"
                     /translation="MSAQITVHVADTQRSVAQGTTAGELFEGDRSVLVARVDGELRDL
                     AHVLVGGEHVEPVTAAEQDGLDVLRHSAAHVLAQAVQEVHPDARLGIGPPIRDGFYYD
                     FDVATPFTPEDLKALEKVMQRIINEGQTFHRREISDEDALVELAGEPYKCELIGLKGG
                     ASEEAAEGAAVEVGGAQLTIYDNVRRDGSRAWGDLCRGPHVPSTKVLGNAFKLMRSAA
                     AYWRGSEKNPQLQRVYGTAWPTKADMVAYLDRLAEAEKRDHRKLGAELDLFSFPDEIG
                     SGLPVFHPKGGIIKREMEDYVRRRHIEEGFEYVGTPHIAKEGLFHTSGHLPYYAEGMF
                     PALDVDAGPYRLKAMNCPMHNLIYRSRGRSYRELPLRLFEFGSVYRNEKSGVVHGLTR
                     VRGFAQDDSHSYVTAEQAPAEIKHLLDFCLGLFRDFGLDDFYLELSTRDETGDKKDKF
                     IGSDEQWAVATGVLEDVCRESGLELVADPGGAAYYGPKVSVQARDAIGRTWQMSTIQY
                     DFNQPERFGLEYQASDGSRQQPVMIHSAKFGSIERFIGVLVEHYAGAFPVWLSPVQVV
                     GVPVAEEFNDYLWDVLSQMKAQGIRVELDESDDRFPKKIRNASKSKVPYVLIAGEEDR
                     SAGAVSFRYRDGSQKNGVPIADAIAEITAAIADRRQV"
     gene            11912..13807
                     /locus_tag="PL014_02700"
     CDS             11912..13807
                     /locus_tag="PL014_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT56035.1"
                     /translation="MRTLPHGTWPSPVTAASLAQGQAGLDEVRLDGPDTYWLEARPWE
                     AGRVALVRHDGRTGERQDAVAAPWSVRSRVHEYGGGAYAVRDGLVVFSTFADGRLRRT
                     RPSQPDTDPEPITPEAAVRYGGLVLTGDAVFAVREDHRGGGEPVNELVRLSPQGPNAD
                     LGQVLVTGPDFVSRPAVSADGTQVAWVQWDHPNMPWDSTTLLRATVTDAGLTDVRVVA
                     GGEGISVVQPQFGPDGTLWFVSDESGYWNLHRDAEASPSPVHALDADVAAPQWVLGMS
                     DYAVVDEGRVLIRFWREGTARLAVLDPRDGTLVDLDRPESGFDQLVTDGSEVALRTAS
                     AREHPRVIRGVLTADGALDATVLATASESAPDPAHLSTPQAWTWTNSAGLDVHGFAYP
                     PANADVSAPAGELPPLLVLVHGGPTSHSEPVYKAQTQYWTSRGFLVLDVNHGGGTAYG
                     RDYRGRLTGQWGVVDIDDVVCGVRSLADAGRIDRDRVAIRGGSAGGYTVLRALTSSTV
                     FAAGASYFGISDLAALLVDDHKFESRYTTTLVGPWPQAEQTYRERSPVHHIADLHGEL
                     LLLQGADDLVVPVAQAQLMADAMRDAGKDVELVVYEGEGHGFRQASTIIDSLERELAF
                     YLRVFEG"
     gene            complement(13829..14731)
                     /locus_tag="PL014_02705"
     CDS             complement(13829..14731)
                     /locus_tag="PL014_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT56036.1"
                     /translation="MIDLVAVESLVAVERCGSVVGAADELGFTPSAVSQQVKRLERQV
                     GVPLLDRAGRGVVLTAAGRRLVDEGRSLRVAVEGLRSRLQGEGGRPSGLLRLAAFSTA
                     VRGIVPPMLDALRASAPDLDVIVHEVEPWDAVAEVAAGRTDMAIVHRWEGMRFHVPPT
                     VRGEPVLTDVADVLVHVDDPLASQESVTPADLMTHLWVSTPEGTICHEWFSHMFAATP
                     QAPRVGFRCVEFASQVELVAHGLAVALVPRLGRGHLPSTVVAVPVLDPVPTRRVDVHW
                     RASMGESPAVDAVRAALREVSRSI"
     gene            14804..15781
                     /locus_tag="PL014_02710"
     CDS             14804..15781
                     /locus_tag="PL014_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT56037.1"
                     /translation="MPRRHVALAVLVTVIWGANFVVIDEGLGDIPPTLFVAMRFVVVL
                     IPAIFLVPRPAMPWRDVVTIGAFMSLGQFGLLYTGLAVGMPAGLASLVLQAQVVATVI
                     IASLVLHERPSRTQVIGVGIGLLGLVVVGVGRSAATAALGLLFVIAAALSWAIGNVAA
                     RRAAAHTPPTASRSGGLAAGLSLTVWSATVVPLPLFALSLALDGPDAVAQALTHPTVP
                     AVLSTIYTAWLASLVGYGIWNTLLAHHPAAAVVPFTMLVPVVGLTTAWLVQGEVPNGW
                     ESVGGALLLLGVAVTTGVLGPRSRLGPRVGPGVEPPGREPAGGDPTPAR"
     gene            complement(15672..17063)
                     /locus_tag="PL014_02715"
     CDS             complement(15672..17063)
                     /locus_tag="PL014_02715"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HOT56038.1"
                     /translation="MVSREDELVEPRGLPSRVRATLGGWASAVRAALTTPGVERAEGL
                     LMLKAAVATVLAWQLAVHLLDSPTPFYAPMAALLVVDRTMVRSIGASARRVAAVVLGM
                     SIAWLVGSLVGVTWWSMVPVMMAALLIARWPLLGDHGIQVPTMALLSLLTVKGTDTDF
                     TYLTIVETVLGGIVGVAVNAVVLAPMHLDEPRDALRDLTTRVQDLLKDMASGLREGFD
                     ADRARRWYDTATDLGDRVPEVLGVVETGRESTRFNWRHRLRPARIDWDGYTRTVEAVR
                     RAQWQVGSIARTLVDAADEAARHPAPSAGWLDDYAGVLDEIGDAISDFGVWTEPSREA
                     VEQHVQRALDTLDDLSEQVRVTPLDDPRAWPTYGALVLEAERLARELRESNSDASVPT
                     DTGPIRTPLAETVPAVAQLQQQIEQVPLVGEHLPRIIEQVSGRPPQAPGPAAPPPVRP
                     SAPADCEGPAPRS"
     gene            complement(17127..17978)
                     /locus_tag="PL014_02720"
     CDS             complement(17127..17978)
                     /locus_tag="PL014_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOT56039.1"
                     /translation="MATEILSGVPSTAPLLVRAALTSRGRRGDAVPDRTLVVEDVRID
                     RAKLAAYQRLTGYAVDDTVPQPYPWVLAFPVQTALMARPDFPMPLPGMVHLENRVTSH
                     RAMDAGDPLTLSVSAGQIRPHRRGRTLDVRLEARVGGELVWECDSVYLSRGRGTQDAP
                     RGDGPPPPPGGQPVAQWRLPEDLGRSYASVSGDVNPIHLHPLTAKAMGFPRHIAHGMW
                     TCARTLAALGRTSLGPSTSHVWFTKPVFLPSTVELVVDREPARTVAALRSAKSPETTH
                     LVLTLEV"
     gene            complement(17980..19329)
                     /locus_tag="PL014_02725"
     CDS             complement(17980..19329)
                     /locus_tag="PL014_02725"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HOT56040.1"
                     /translation="MGHRDGYTAFVSSGVGKKISTTLGLPQPVALRRHVAGRPLVDGP
                     LLVGGHGSIPALAAMRAKLGQAGALIVDEVPQEGRLGGVVVDVSAATTPTDLESLRAT
                     LAPALKRLGRCARVVILGADPASTGSAAERATHRALEGITRSVAKELRAGATANLVLL
                     TPGSEGAALGTVLFLLSARSAYVDGQVFRIGVATSQEPADAAAPLAGKVAVVTGAARG
                     IGADIARTLARDGARVVCVDMPSAGASLAKVANSIGGSALQADVTSPDAGARIMNHAR
                     TRHGGLDIVVHNAGITRDKLLANTDAERWGSVLAVNLTSILAMNEQFLARDGLRDGGH
                     VVLVSSIAGIAGNRGQANYAASKAGVIGLVDAYAADKGLRKRGITANAVAPGFIETEM
                     TARIPFATRELGRRLNSLAQGGLPIDVAETIAHFASDAQSGVNGNVVRVCGQSLLGA"
     gene            complement(19331..20614)
                     /locus_tag="PL014_02730"
     CDS             complement(19331..20614)
                     /locus_tag="PL014_02730"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOT56041.1"
                     /translation="MTTPRRAAVLGGNRTPFARQFGAYAEAANQDMLTAAIEGLVARH
                     GLAGERLGEVVAGAVIKHSRDFNLTREAVLGSSLSPTTPAYDITQACGTGLEAAILVA
                     NKIALGQIESGIAGGADSASDAPVVVTESLRRKLLRLNRAKTSADRVKAALAFRPTDV
                     GVSVPANTEPRTGLSMGDHMALTAAQWGVTREAQDELALASHVNLAAAYERGFFDDLL
                     TPYLGLTVDQNLRPGSTLEKLGSLKPVFGKGEGATMTAGNSTPLTDGASAVLLGSQEW
                     ADAHGLKPLAWFVDGETAAVDYVGGDEGLLMAPSYAVPRLLARQGLRLQDFDFYEIHE
                     AFAATVLSTLAAWEDDDFCRDRLGLHAPLGAIDRSRLNVAGSSLAAGHPFAATGGRIV
                     ATLAKLLHEKGSGSRGLISICAAGGQGVVAILEAA"
     gene            20695..21423
                     /locus_tag="PL014_02735"
     CDS             20695..21423
                     /locus_tag="PL014_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT56042.1"
                     /translation="MDVTQAPPDRRRSRWDEHRLVRRAELVEATLLAIRTHGAGVGMD
                     EVAATARTSKTVFYRHFTDRAGLYTAVAERVDATIIRDLTRAAADPASDGSRDGGRAV
                     IRGVIAAYLHLVEDDPQVYRFIVNAPMVPAGERPEGDVAAGMTGRIATHVADLVAGGL
                     ASGSGRPSGSAAPPVPALDARACQLWGVALVGMVRAVADSWMAEGGARNGPSSDELAD
                     QLTALVWEGLPDVAAGAQHGSGRS"
     gene            complement(21635..21708)
                     /locus_tag="PL014_02740"
     tRNA            complement(21635..21708)
                     /locus_tag="PL014_02740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21672..21674),aa:Val,seq:cac)
     gene            complement(21773..22216)
                     /locus_tag="PL014_02745"
     CDS             complement(21773..22216)
                     /locus_tag="PL014_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HOT56043.1"
                     /translation="MTPEERRHVLRDANDEDDWAWRRAIRANPTSRRIYRIAVFVVGL
                     VLLLGGLALVPLPGPGWLIVIAGIAIWASEFEQAARLLDFVKAKVRAWEQWVRRQPLW
                     IKGLVGLATAAFVGCVLWALLVVSGVPGFLPTSMVTWLEGNVPGL"
     gene            complement(22213..23055)
                     /locus_tag="PL014_02750"
     CDS             complement(22213..23055)
                     /locus_tag="PL014_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT56044.1"
                     /translation="MTDIRIWVNGELVDADTPAVSAVDHGVTVGDGAFETAKIDRGAP
                     FAVTRHLRRLDRTMAGLGLPPADHAQIHDGIRAVLDGEVIDFGRLRFTVTGGKGPLGS
                     DRLDSALTHIVTAVAHERPEAQGSVVTVPWVRNERAATAGLKTTSYADNVIALAYAKE
                     RGAVEAIFANTRGELCEATGSNVFVVLDGTLRTPPLNSGCLAGITRELVLEWCREDGL
                     DIVEEAMPLDVLEHADEVFLTSSIKDVFPVSAVDGRELGAPGPVTSAAREVWARHAAR
                     GIDP"
     gene            complement(23059..24102)
                     /locus_tag="PL014_02755"
     CDS             complement(23059..24102)
                     /locus_tag="PL014_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOT56045.1"
                     /translation="MSEARFTSLVARGASAVSHDIDDLTSGFWVVVITFEGELTAVRM
                     DDVDRTEPVRRREGRSDVGAWPALSGPWRTSLDRTAYVEGVSQIRGRIEAGTVYQVNL
                     CRVLEHDLDEGADIAVLGDRLAAGNPAPYSATIALPEAGLELACASPEAYLLRQGDLV
                     TSRPIKGTATTPELLLAKDYAENVMIVDLVRHDVGHVSRPGTVSVDHLCRVEEHPGLV
                     HLVSDVSGRLRPGTSWRDLLDASFPPGSVSGAPKHTALLAIADLEPVGRGPYCGAVGW
                     IDIDRDEAELAVGIRTFWVARDGDGRRVLRFGTGAGITWGSDAQGEWAETELKAARLV
                     ALAEGEAPARAKV"
     gene            complement(24132..24782)
                     /locus_tag="PL014_02760"
     CDS             complement(24132..24782)
                     /locus_tag="PL014_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HOT56046.1"
                     /translation="MTDHAEVVDHLHLVDRVLALRAATAGRVVIIGIAGEPGAGKSTL
                     AASVVAALTDRGCAAVILPMDGFHLANVALTSLGLAERKGALETFDAAGFAALLARIR
                     AEGPDTVWAPTYDRSVEESIAGAIPVPPGTEVVVSEGNYLLVDEGPWAQVGELLDEVW
                     AVRVDAAIRTERLIARHVRYGRTPAEAAAWVASVDEPNAAVIRATLGRADLDVSTG"
     gene            complement(24900..25412)
                     /locus_tag="PL014_02765"
     CDS             complement(24900..25412)
                     /locus_tag="PL014_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56047.1"
                     /translation="MARALVVYESMFGNSEKVARAVAQGVAEVMPVKLVEVTVAPTEL
                     EGVDLIVAGGPTHAFSMSRESTRRDARVQGASQGSVATGLREWLASLPDEHGRWFAAF
                     DTRVSKVRRLPGSAAHSAARAGRRHGFSQAIAPQSFYVLDVEGPLADGELEHARVWGA
                     AVAASVPTTV"
BASE COUNT         3650 a         9193 c         9159 g         3551 t
ORIGIN      
        1 gccgggttgc ccgacttgaa gatgccggag ccgacgaaga cgccctcggc gccgagctgc
       61 atcatcatcg cggcgtccgc cggggtggcg atgccaccgg cggtgaagag caccaccggg
      121 agcttgcccg cggcggcgac ctccttgacg agctcgtacg gggcctgcag ctccttggcc
      181 gcgacgtaga gctcgtcctc gggcaggttc tgcaaccgac ggatctcttg gcggatctgg
      241 cgcatgtgcg tcgtcgcgtt cgagacgtcg ccggtgccgg cctcaccctt ggagcggatc
      301 atcgccgcac cctcggtgat gcggcgcagc gcctcgccga ggttggtcgc accgcagacg
      361 aagggcaccg tgaaggccca cttgtcgatg tggttggcgt agtccgccgg agtgagcacc
      421 tcggactcgt cgatgtagtc gacccccagg ctctgcagca cctgcgcctc gacgaagtgg
      481 ccgatgcggg ccttggccat gacggggatc gagacggcgg cgatgatgcc gtcgatcatg
      541 tcggggtcgc tcatccgcga cacgccgccc tgggccctga tgtcggcggg cacccgctcg
      601 agggccatga cggcgacggc accggcatcc tcggcgatct tggcctgctc ggcgttgacg
      661 acgtccatga tgacgccacc cttgagcatc tcggccatgc cgcgcttgac gcgggtggtg
      721 ccggtgccgg gggcgctctg ctgctcagtc atggcgccaa gtgtaggtcc gcggcgacgg
      781 tgctcgaacc gctgtccgga caccctcagc ccgggctcag gcgggcaggg ccagcgggac
      841 gtcgtcgtcg aagacgacga gctccggaag ggcggcgtga ccggccaggc cgaaccagcg
      901 cacggtgcgc ttggcccgca cgcggcgcac gtcgcgcacg gcgtcgttgt ggaagcgccg
      961 ggccagctgg acccgcagca cgctggccgc gacccggtcg agctcgtcga tcacgaactc
     1021 gtcgccggcg gcccgcagct ccaccaccac ctcacccggc agggccgccg ccagcgccgc
     1081 cgtgaggtcg ctctcgacgt gttcgcggcc cccgaagtgc tggccgtcga gcaggtcgtc
     1141 ggtcaccggc tgctccgtgg ttcgctcgag cgactcggtc gcggcgtcgg cgatgagcag
     1201 ggcactggcc gggtcgagga cgccgctgtt cgcgagctcg agcgcggcct cggcccgccg
     1261 gatcagctgt gcgtccagcg cggacatcgc cccttcgacc ttggcgtgca gccggtcgag
     1321 ccgcgcggcg gagtacgaca ggtaccaggc ggccacgagc aggacgatga cgacgaaggt
     1381 cgcgatctgc agggtgtcgt tcatcagttg ccgcctcccg ggacgccgcg tacgagccgg
     1441 ccccacaacc cctggggtgc gggttcatcg ggttcggcgt cggcgccggc gatcacggtc
     1501 tcgtagacgg ccatcacctt cgcggcgacg acgtcccagt cgaagaccct cgacctcgcc
     1561 cgcccggcga ctcgcagcgc ctcacgccgg gccgggtccg cgagcagcgc cagcagcgcc
     1621 tgcgccaggc cctgcgggga cccggtctcg aagaggatgc cgttggcgtc accgtccgcc
     1681 ggcgtcgcac cgacgtcgag gacggctgcg aaggcgggca ggtcggacgc gacgaccggc
     1741 gcccccgcgg ccatcgcctc gacgaggacg atgccgaagc tctcaccccc gaggtgcggg
     1801 gcgacgtacg cgtcgaccga cctcagcagc gaggccttgt cgtcgtcggt gaccatgccg
     1861 agcatcaccg tcgccgcagc caccctcggg tcggcgtcct tcagcaggtc ggaggcgtcc
     1921 ccgggccccg cgacgagcag gcggatgccg gggtgcacct cgagcagcgc cggcatcgcc
     1981 tggagggcga tcgccaagcc cttgcggggt tcatcgaccc gtccgaggaa ggccagcgtg
     2041 ggcgcctcgg aggttccggc ccactccggc cgcaccggtg ccagggcgaa cgagtcgacg
     2101 tgcaccccgt tggggatgat gaaggccatt cccccgaggt ggcgctgcac ggtgccgagg
     2161 gcgtcctcgg acacggcgat ccgcccgcgg atcttctcca gcgagggccg cagcaggggg
     2221 tgcgccgcgt gcagcgcccg ggaacggatc atcgcggagt ggaacgtcgc gacgacgggt
     2281 tcggtcgagg cccacagcgc caccagggac accgaggggt tcaggggttc gtgcaggtgg
     2341 agcacgtcga agttcccggc ctcgagccag cggctgaccc gggtggacgt ggcggggccg
     2401 agggtgaccc gggccaccga cccgttgtag cgcacgggga cgggacgccc gaccgacgtc
     2461 acgtagtcgg gcaggtcggc gtcgctctcc gcgggtgcca ggacccacac ctcgtgcccc
     2521 tggcggatga agtgttccgc gatgtcacgg acgtggtact gcaccccacc gggcacgtcc
     2581 cacgcatagg ggcagaccat gccgatcctc atgcggcacc gtgcgcggcc ctcagccgcg
     2641 cagggtcgag gtcgtcgatg aagacgcgct gcatcatgtg ccagtcactc gtgtgctgcg
     2701 tgatgacggt gccgagcgcg tcggcacagg cctgggtcat cgccgtggcc cgctcccgcg
     2761 tcgtgcccgt ctcgggcacg gggacctgct ggtggaaggt gatgacgatg ccccaaccgc
     2821 tcccgcgacg ttcgtggcgg atcgtcacgg ggtacagggg ccggcgctcg gcaagggcca
     2881 gggcggccgc gccgggcgcc atccgcgccc ggtgaccaca caggtcgacc tcgaccccac
     2941 ggtcggtgag gtcgcggtcg gccagcaggg ggatgatccc ggggcgcctc gacgcctcgc
     3001 gcaacgccgg gaacgggttg accccaccgg tgagcgggat gatcgtcatg cccaaggact
     3061 cccggaaggc caggaactcc tcgaagacct cctcgggttt caggcgctcg gccaccgtgg
     3121 tgacggcccc gaggtgcgag gtcgcccagg ctccggcgag gtcccagttg cccaggtgcc
     3181 cgaggaaggc cacgaccgag ccacccgcgt cgatcacctc gcgcaccggg ccgtcaccct
     3241 ccacccgcac ccgggccgcg aggtcaccga ccccgaggtc ctggaggcga aaggcctcgc
     3301 agtagtaccg caggtaccgg cgcatgccgt cgcgcacgag ggcgtcgagg gcggcgtcat
     3361 cgagctcagg gcgcacccgc gcgtagttcg accgcatccg ggcggcgctg ccgcgggcga
     3421 cggccacgtc ggcgacccgg tcgaacagcg cgtaggcgag gcgttccggc atccgacgca
     3481 ctgcccgcca gccgagctgg tgaccggcga gggtcagacg gtcggcggcc gacgccgaga
     3541 gcagcctcac agagccaggg cctgtcggcg cacctcgagc atccgttgga ccacggtgat
     3601 gagactggcc agcgccagca gtgccagcac cacgccgagg tagatccacg gcaggtcgag
     3661 cagtccggtg aacccggtgg cgatgaggac caccacgagg cggtcggccc gctcggcgat
     3721 gccgacgttc gccgtcatcc ccagcccttc ggcacgcgcc ttggcgtacg agacgacgct
     3781 gccgaggatg aggcaggcca gcgcgagccc cgccatgacg ggcgcgagtt cgccctcctg
     3841 gcgcccggct cccgcgaagt agagcaccag gcccccgaag atggcagcat cgccgacccg
     3901 gtcgagcgtg gagtcgaggt aggcgcccca gttgcccgag cgtccggtcg agcgcgccat
     3961 gatcccgtcg agggtgtcgg agaagacgaa gaaggtgatg acgagggtgc cgacgagcag
     4021 ctcgccgcgg gggtagaacg ccagcgcccc gaagcacacc cccagggtgc cgatgaccgt
     4081 cacgacgtcg gggctgatgc cgaggcggac gaggaccgcg gccacggggc tcagcagctt
     4141 ggtgaaaaag gcgcgcgcgt aacggttcag catggtgcac ccagcgtaac cgcgcgccga
     4201 cgcgtctctg gtcacaccgc acgcttctgg cttgtgcgac gacgcacaga ggagaaatgt
     4261 aggaggcatg aacggcaccg cgaccaaggc cccgaccgtc accaccacga ccagcatccg
     4321 caacgtcgtg ctcgtcgggc cgagcgggtc gggaaagtcg agactgttcg accacatcgt
     4381 cggttcggcc gtgcccgcac gcgcaccacg aagtgacggc ggcgatgccg gggacgcgac
     4441 aacgggcctg cgggcggcga ccatcgccac gggatcggtg gtggtgaccc ttctggacgc
     4501 cccgggcaac cccgacttcg tcggggaggt gcgagcaggg ctgagggcgg ctgatgccgc
     4561 cctcttcgtc gtctcggccg ccgacggcgc agatgccgcg agccgtgccc tgtggcacga
     4621 gtgcgcagtg gtcgggatgc cgcgtgcggt cgccatcacg gcgctcgacg cccgcgatgc
     4681 tgacttcacc gccacgctcg ccgactgcca ggcccacttc ggtgccggca tccagccgct
     4741 cggcgtgccg gtagccgggg acgccggcgc ggtgacctcc atcgccgatc tcctgctcgg
     4801 tgagatccac gactactccg cgggtgagcg cacggtgcgc cctgccggcc ccgagcacgc
     4861 agaggtcttc gacaccttcc gcccctccct catcgaaggg atcatcgagg agtccgagga
     4921 cggcaccctc atggaccgct acctcgaggg cgaggacctc gacttcgcca ccctggagaa
     4981 ggacctgctc accgcgatcg cgcacggcac cttccacccg gtgctcccac tctcggccga
     5041 gacgggggca ggcgtgggcg tgctcatgca cctcatcgag gccgcgttcc cccacccggc
     5101 actgcacccg ttgccgacgg tcaccccggt cgccgggggc acggcggtcc agctcacggc
     5161 cgaccccgat ggcccgctcg tcgccgaggt ggtgcacacc gagtccgatg cctacgtcgg
     5221 gcaccgctcc ctcgtgcggg tgttctccgg gacgctgcga ggtgaccggc cggtgcacgt
     5281 gtcgggccac ctcgagcggc tcggggtacc gaccggagaa ggtcacgcgc cgcacgacga
     5341 cgacgtgcgc cccggagccc tcgccgctcc cctggacgac gagctgctgc ccaagaccga
     5401 ggcgatcgcc ggagagatcg tcgtcgtcac caagctcggc agcgcccaga ccacggacac
     5461 gctctcctcc cccgaccagc ccctgctcgt gacgccgtgg gccctgcccg aggcgctcct
     5521 gccctctgcc gtggcaccgt cctcacgcgc tgacgaggac cggatgccgg gagcgttccg
     5581 tgaactcgcg gccgaggacc cgtccctgcg gatcgagcac gacgccgcga ccggccaggt
     5641 ggtgttgtgg accaccggcc cggcccatct cgacctcgtc ctggcccggc tgcgcacacg
     5701 gttcaacgtc agcgtcgacc aggtgccggt gcaggtggcg ctgaaggaga cggccaccgc
     5761 ccgggccgag gccctcgggc ggcacgtcaa gcagtccggc ggccacggac agttcgcggt
     5821 ctgccacctc gtcatggaac cgctcccccg cgggtcgggc atcgagttcg ccgaggtcgt
     5881 cgtcggtggc gccgtccctc gccagttcgt cggcagcgtg gagaagggcg tccacaccca
     5941 gctcgagaag gggctgctcg cggggtggcc ggtggtcgac gtgcgggtca ccctcaccga
     6001 cggcaaggcg cactcggtcg actcctccga catggccttc cagacggcgg ccggcctcgc
     6061 cctgcgccag ctggcctccc cgtcgacgat gaccctgctc gagccgatcg acacggtgag
     6121 cgtgagcgtc gacgacgagc acctgggcgc ggtcatgacc gacatcagca cccgacgtgg
     6181 gcagatcctg ggctccgccc ctgcggtcga ccagcccggc cggtcggtgc tcgaggcggc
     6241 ggttccccag ctcgagctgc tcgactacgc cgtctcgctg cgctccctgg cccacggcac
     6301 gggcacgttc caccgcgagc accgtgggta cgaggagatg ccggagcgcc tcgccgcgaa
     6361 gcacctcggc gagcggggtc ggcacgcctg atcgctcact ccaggctggc gccgatctcc
     6421 acgaggcggg tcaggaaggc ccgctcgtcg agacgcttgc gacgcaacca gccggtgacc
     6481 tcgtcgttgc acttgctcgc gttgcacggc ccgcaggccg gcacgacgtt gcgtaccgtg
     6541 tagcgcccgc cgcgcgagat cggcagcacg cagtcgcgct ggagcggacg gtcggtggct
     6601 ccgcagtagg cgcagcccgc ccaggccacc tgcagcgccg accactgctc gtcggtgagg
     6661 tcgtgctcga ccttggccat ccgccgctga cgccgtcggg cggctcgggc gcgccggatg
     6721 ccgctcacgg ggtcggccag tgctccacca ggcggctgcg gacgtcctcg agcagcgccg
     6781 gcaacgcctt ggtgcgggcg atgatcggca ggaagttggc gtcgccgccc cagcgcggca
     6841 cgacgtgctg gtgcaggtgc gcggccaccc cggcaccggc cacggcgccc tggttcatcc
     6901 ccaggttgaa gcccatcggg gccgaggctg cctccagtgc cctgatcgtc gccttggtca
     6961 gggcggtgaa ctccagggtc tcctcgtcgg tgaggtcgac gtagaggggg acgtggcggt
     7021 acgggcagat cagcacgtga ccggggttgt agggaaagag gttcatcacg acgtagcagt
     7081 gctcgccacg gtggacgatc aggcccgcgg cgtcgtcctt gcccggcgct gcgcagaagg
     7141 ggcagccgtc gccgggctcg gcatcgggcc ggtccgcctg gatgtagacc atccggtgcg
     7201 gggtccacag ccgttcgaag ccgtcaccct ccccggcgaa gtcagcggca tcctcatgcg
     7261 tcggcatgag cgtgatccta ggacgcggct gccgcgctgc tccgcaacgc cggtgctgtc
     7321 gccgccgggt cgaggtatga ccgcccgtga accgtgcacc cgcgattcga ccccggcgcg
     7381 agctgctccg ttcagcgcag gtggctggcg ccgttgaagt ccagcacggc ccccgaggcc
     7441 cactcggcac ccggggacgc cagcgtcacg acggcggcag cgacctcctg cggtgtggcg
     7501 acccggtgga acgggctctg cgcccgcgtc gcctcccccg ctgcaccctc gagcgcggca
     7561 ccgaccatct cggtctcgat gaacccgggc gcgatgctcg tcacggcgat gccgtgcggg
     7621 gccaggcgct gcgccatcga ctggccgaac gagtgcagac cggccttgct ggcgccgtac
     7681 gcggggatgt cgggctcccc ccggtatgcc cctctcgagc cgacgttgac gacccgcccg
     7741 acggggaccc cgtcagccgg tggcacgtcg atcatctgcc gggccacgca ccacgtgagg
     7801 ttcgccgggc cgagcagatt cgtcgcgagg gtgcgctgcc agatctgcac ccagtcctcg
     7861 taggacgtga gctcgagcgg gtggtcaccc cgccggctcc cgtcgactcc cggtgcaggc
     7921 ggcacgatca tggcggcgtt gttgacgagc acgtcgaccc ggcccagcgc ggtggccgcc
     7981 tccgacgcga ggcgcgccac ggcatccggg tcggagaggt cggcggcgag ccgcacgtgc
     8041 ccgctccccg gcaggccctc gagcacagcc gccgcccgat cgagcgagga cgggccccgg
     8101 tggtgcacga cgacccggtc gccgcgctcg gcgaaggcgc gagcaaccgc ggcccccacc
     8161 cctcgcgatg ctccggtgac gaggatgccg cgcgacggtc cggctgcgga ggtggattcg
     8221 ctcatggacg gcaccctatc cacgcaggtc gggcaggtcc tcggccgcga tctcggcgag
     8281 cagggcgcgc aggtcgtcca tgatcaccgc cgtcatggcc cgtaggtcct ctgggtccgc
     8341 gacgcgggca cgcaactgcg ccggagtgat cgcctcgccc accgcgacga ccaccgtcgg
     8401 acggcgcgag agccgccctg ggcgcagccg cccgagccag cgcagcggcc gcacgaggga
     8461 ggacgttccc tcccgagtga gcaggcggtg cgctccccac tgggcgacgg ggacgatcgg
     8521 gcagttggcg gtcagcgcga gccgcaccgc cccggtgcgc acctcgctca tcggccgata
     8581 gtcgggctcg gtcgtgatct tgccctcggg gtagatcgcc acggcccacc cccgcccgag
     8641 tgcgtcgaga gccggctgca gcgccgccgc cgggtcgcgg tcgcggcgca ccgagatctg
     8701 cccggttcgc cgcagcagcc acccgacacc gggccacgcg aacgcctcgg ccatcacgag
     8761 gaaggtgccc tggcggccgg ctcgccgcag ggcgctcgcg agcaccagcg ggtcggagac
     8821 ggagaggtgg ttggagacga cgacgacggc gccggatgcc gggatgcgct ccacgtggtg
     8881 gtggtcgcgt cgggtcagca cgccgagcgc tgcatcgacg atggccacgc cgaccgagta
     8941 gaggatcacc cgccccgccg actgatctgg cgctccatga gctgctgcga cgggggcccg
     9001 aacccgaagc gttcgaagat ggtgcgcacc cccggcaggg cgtgcagctg cacgcggctc
     9061 accccgtcgc cctcggccca cgcgagcatg gcgtcgagca gcccttcgcc gatcccggca
     9121 ccgcgcacac caatgctcac gaagagcaga ctgacgtgga accacgcatc ggcgggacgg
     9181 tgggcgctgg gcaggcgctg cacccgggtg ccgtgcagca cgccgagcgg ccgccctcgc
     9241 gggtcctcgg ccagccacgt gcgacgagcc gtgtcgcgca gccacgcctg cgcgaactca
     9301 tccaggaacc cgacgggcac cgcgtgcccg agttcgcgct cgtgctggat gtgcagggcc
     9361 gcgatggcga aggcgtcaac cggacgcgcc tcgcgcaccc gccaccccga ggtctgcgca
     9421 gcgtcgccgt ccatgtgccc agcatggacc acgctgctgc tgtgctcacc cgatgcacgc
     9481 cgggcagtcg ctcacccgat gcccgccgga caaagggtga ggccccggga agtatgagtt
     9541 cccggggcct cgtgactcgg catgatgctc cgtgtctcac gagatggttt ccgctcacct
     9601 ctgaccctca tcaccgggtc agttcgtgcg gcaagccgct cgagtggtgt tcgttcttct
     9661 ctcggttcct ccagtcactt tcgtggtgga gtgcttcatt tctacgcctg cgcgcgagcg
     9721 tcccgcaagg ggttccggga ccaaatcacg gcaagattct gcggtcggcg tgtcgctcgg
     9781 cgtgtcgcgt gacgggtgtg acgagggtgt tcagacctgc cgccggtcgg cgatcgccgc
     9841 ggtgatctcg gcgatggcgt cggcgatcgg caccccgttc ttctgcgacc cgtcgcggta
     9901 acggaaggac accgcgccag ccgagcggtc ctcctccccc gcgatgagca cgtaggggac
     9961 cttggacttg ctcgcgttgc ggatcttctt cgggaacctg tcgtcgctct cgtcgagctc
    10021 gacgcggatg ccctgggcct tcatctgcga caggacgtcc cacaggtagt cgttgaactc
    10081 ctccgccacg ggcaccccga ccacctgcac cgggctcagc cacaccggga aggccccggc
    10141 gtagtgctcg acgagcaccc cgatgaagcg ctcgatggag ccgaacttgg ccgagtggat
    10201 catcaccggc tgctggcgtg acccgtcgga cgcctggtac tcgaggccga accgctcggg
    10261 ctggttgaag tcgtactgga tcgtcgacat ctgccaggtg cggccgatcg cgtcacgggc
    10321 ctggaccgac accttggggc cgtagtacgc ggcgcccccg gggtcggcga cgagctcgag
    10381 cccggactcg cggcacacgt cctcgagcac cccggtcgcg acggcccact gctcgtcgga
    10441 gccgatgaac ttgtccttct tgtcgccggt ctcgtcgcgc gtggacagct cgaggtagaa
    10501 gtcatcgagc ccgaagtcac ggaacaggcc gaggcagaag tcgagcaggt gcttgatctc
    10561 cgccggtgcc tgctcggccg tgacgtagga gtgcgagtcg tcctgggcga aaccgcggac
    10621 ccgggtgagg ccgtgcacga ccccggactt ctcgttgcgg tagaccgacc cgaactcgaa
    10681 cagtcgcagc ggcagctcgc ggtacgagcg cccgcgcgag cggtagatga ggttgtgcat
    10741 cgggcagttc atcgccttga ggcggtacgg gccggcgtcg acgtcgaggg ccgggaacat
    10801 cccctccgcg tagtacggca ggtggccgga ggtgtggaac agcccctcct tggcgatgtg
    10861 cggggtgccg acgtactcga acccctcctc gatgtgccga cggcggacgt agtcctccat
    10921 ctcgcgcttg atgatgccgc ccttggggtg gaacaccggc agccccgagc cgatctcgtc
    10981 ggggaagctg aacaggtcga gctcggcccc gagcttgcgg tggtcgcgct tctccgcctc
    11041 ggcgagccgg tcgaggtagg ccaccatgtc ggccttggtc ggccaggccg tgccgtacac
    11101 gcgctgcagc tgcgggttct tctccgagcc gcgccagtag gcggcggcgc tgcgcatgag
    11161 cttgaacgcg ttgccgagca ccttggtcga ggggacgtgc gggccgcggc acaggtcgcc
    11221 ccaggcgcgg ctgccgtcac ggcgcacgtt gtcgtagatc gtcagctggg cgccgccgac
    11281 ctctacggct gcaccttcag cggcctcctc ggaggcaccg cccttgaggc cgatcagctc
    11341 gcacttgtag ggctcgccgg cgagctcgac gagggcgtcc tcgtcggaga tctcacggcg
    11401 gtggaaggtc tggccctcgt tgatgatgcg ctgcatcacc ttctccaggg ccttgaggtc
    11461 ctcgggcgtg aacggggtgg cgacgtcgaa gtcgtagtag aaaccgtcgc ggatcggcgg
    11521 tccgatgccc agtcgcgcgt cggggtggac ctcctgcacg gcctgcgcca gcacgtgggc
    11581 agccgagtgg cgcagcacgt cgagaccgtc ctgctcggcg gccgtgacgg gctcgacgtg
    11641 ctcgccgccg acgaggacgt gcgccaggtc gcgcagctcg ccatcgaccc tagcgacgag
    11701 gaccgagcgg tcgccctcga agagctcacc cgcagtcgtg ccctgcgcca ccgatcgctg
    11761 cgtgtcggcg acgtggacgg tgatctgggc agacatgggt gtggctctcc tggtgcggaa
    11821 ggggtagcgg cccgtcggga ccgcactcca gcgtatcgag cggtacgcgg atgccgcgaa
    11881 acggcatccg cggtcggcgt acggtgctct cgtgcgcacc cttccccacg gcacctggcc
    11941 ctcccccgtc accgccgcct ccctcgccca ggggcaggcc gggctggacg aggttcggct
    12001 cgacgggccg gacacctact ggctcgaggc gcgcccgtgg gaggcgggcc gggtcgcgct
    12061 cgtgcgccac gacggccgca ccggagaacg gcaggatgcc gtcgccgcac cgtggtcggt
    12121 gcgctcgcgc gtccacgagt acggcggcgg cgcgtacgcg gtgcgcgacg ggctcgtcgt
    12181 gttctcgacc ttcgccgacg gccgcctgcg gcgaacccgg cccagccagc cggacaccga
    12241 tccggaaccg atcacccccg aggcggccgt gcgctacggc gggctcgtcc tcaccggcga
    12301 cgccgtcttc gcggtgcgcg aggaccaccg cggcggcggt gaacccgtca acgagctggt
    12361 ccggctctcc ccgcagggcc ccaacgccga cttggggcag gtgctcgtca ccggcccgga
    12421 cttcgtctca cgcccggcgg tctcggcgga cggcacgcag gtggcgtggg tgcagtggga
    12481 ccacccgaac atgccgtggg actccacgac gctgctgcgt gcgacagtga ccgacgccgg
    12541 tctgaccgac gtgcgggtcg tggccggcgg tgagggcatc tcggtcgtgc agccgcagtt
    12601 cggcccggac gggacgttgt ggttcgtcag cgacgagtcg ggctactgga acctgcaccg
    12661 cgacgcggag gcgagcccca gccctgtgca cgctctcgat gccgacgtcg cggcacccca
    12721 gtgggtcctg ggcatgagtg actacgccgt ggtcgacgag ggccgagtgc tcatccggtt
    12781 ctggcgcgag ggcacggcgc gcctggcggt gctcgacccg cgcgacggca ccctggtcga
    12841 cctcgaccgc ccggagtcgg ggttcgacca gctcgtcacc gacggaagcg aggtcgccct
    12901 gcgcaccgcc tcggcacgtg agcacccccg ggtcatccgc ggcgtgctga ccgcggacgg
    12961 tgcgctggac gcgacggtgc tcgcgacggc atccgagagt gcaccggacc cggcccacct
    13021 ctcgaccccg caggcgtgga cctggaccaa cagcgcgggg ctcgacgtgc acgggttcgc
    13081 ctacccgccg gccaacgccg acgtcagcgc ccccgcggga gagctgccgc cgctcctcgt
    13141 cctcgtccac ggtggcccca cgtcacacag cgaaccggtc tacaaggcgc agacccagta
    13201 ctggaccagc cgcggcttcc tcgtcctcga cgtcaaccac ggcgggggca cggcgtacgg
    13261 gcgcgactac cgggggcgac tcacgggcca gtggggtgtc gtcgacatcg acgacgtcgt
    13321 ctgcggggtt cgttcgctcg cggatgccgg tcgcatcgac cgtgaccgcg tcgccatccg
    13381 cggcggcagc gccggcgggt acaccgtgtt gcgggcgctg acgtcgagca cggtcttcgc
    13441 cgcgggcgcg agctacttcg ggatcagtga cctcgcggcg ctgctcgtgg atgaccacaa
    13501 gttcgagtcg aggtacacga cgacgctcgt cggcccgtgg ccgcaggcgg agcagaccta
    13561 ccgggagcgc tcaccggtgc accacatcgc tgacctgcac ggcgagctgc tgctcctcca
    13621 gggcgccgac gacctcgtgg tcccggtggc gcaggcccag ctcatggccg atgcgatgcg
    13681 tgacgccggc aaggacgtcg agctcgtcgt ctacgagggc gaggggcacg gcttccgcca
    13741 ggcgtcgacg atcatcgact ccctcgagcg ggagctggcg ttctacctgc gggtattcga
    13801 gggctgaccc caacttcgag ggcggacctc agatactcct cgacacctcc cgcagggccg
    13861 cgcgcacggc atccacggcg ggcgactccc ccatcgacgc gcgccagtgc acgtcgaccc
    13921 gcctggtcgg cacggggtcg agcacgggca ccgcgacgac ggtggagggc aggtgccccc
    13981 gacccagcct gggcaccagg gccaccgcca gaccgtgggc cacgagctcg acctgcgagg
    14041 cgaactccac acaccgaaaa cccacccgcg gcgcctgcgg ggttgccgcg aacatgtggc
    14101 tgaaccactc gtgacagatc gttccctccg gggtggagac ccagaggtgg gtcatgaggt
    14161 cggccggggt caccgactcc tgcgacgcga gggggtcgtc gacgtgcacg agcacatcgg
    14221 cgacgtcggt gagcaccggc tctccccgca ccgtcggcgg cacgtggaag cgcatgccct
    14281 cccagcggtg cacgatcgcc atgtcggtgc gtcccgccgc gacctcggcg acggcatccc
    14341 acggttccac ctcgtgcacg atgacgtcga ggtcgggagc gctggcgcgc agggcgtcga
    14401 gcatcggggg cacgatgcca cgcaccgcgg tggagaaggc cgcgagcctc agcagccccg
    14461 aaggtcgccc gccctctccc tgcagccgtg agcgcaggcc ctcgacggcc acgcgcagcg
    14521 atcgcccctc gtcgacgagc cgccgcccgg cagcggtgag cacgaccccg cgcccggccc
    14581 ggtcgagcaa cggcaccccg acctggcgtt cgaggcgttt gacctgctgc gagacggcgg
    14641 acggggtgaa ccccagctcg tccgcggccc cgacgacgct gccgcaccgc tccaccgcca
    14701 cgagcgactc cacggccacc agatcgatca tgcagcgatg ctaccgagat cgcctcacat
    14761 ttcgttgctg gtgcttcatg gtccatcccg gcaggatgaa caggtgcctc gccgtcatgt
    14821 cgccctcgcc gtgctcgtca ccgtcatctg gggcgccaac ttcgtcgtca tcgacgaggg
    14881 cctgggcgac atccccccga cgctgttcgt cgcgatgcgc ttcgtcgtcg tgctgatccc
    14941 ggcgatcttc ctcgtcccga ggccggccat gccgtggcgc gacgtggtga cgatcggcgc
    15001 gttcatgagc ctgggtcagt tcggcctgct ctacaccggc ctggcggtcg ggatgccggc
    15061 cgggctcgcg tcgctggtgc tccaggccca ggtcgtcgcc accgtgatca tcgcgtcgct
    15121 ggtcctgcac gagcgaccga gccggaccca ggtgatcggg gtgggcatcg gcctcctcgg
    15181 gctcgtcgtc gtcggcgtcg gccggtcggc ggcgactgcg gccctgggcc tgctgttcgt
    15241 catcgccgcc gccctgtcgt gggcgatcgg caacgtcgcc gcccggcgcg ccgccgcgca
    15301 caccccgccc acggcatccc ggtccggcgg gctcgccgcg ggtctgtccc tcacggtgtg
    15361 gtccgccacc gtggtcccgc tgcccctgtt cgcgctctcg ctcgcccttg acggcccgga
    15421 tgccgtggcg caggccctca cccacccgac ggtgcccgcg gtgctctcca cgatctacac
    15481 cgcgtggctg gcctcgctcg tcgggtacgg catctggaac acgctcctgg cgcaccaccc
    15541 ggcagcggcc gtggtgccgt tcaccatgct cgtcccggtg gtcggactca ccacggcgtg
    15601 gctcgtgcag ggtgaggtgc cgaacgggtg ggagagcgtg ggcggcgcgc tgctgctgct
    15661 cggggtggcc gtcacgaccg gggtgctggg ccctcgcagt cggctggggc cgagggtcgg
    15721 accgggggtg gagccgccgg gccgggagcc tgcggggggc gacccgacac ctgctcgatg
    15781 atccggggaa ggtgctcgcc cacgaggggc acctgctcga tctgctgctg gagctgagcc
    15841 accgcgggca ccgtctcggc gagcggcgtg cggatcgggc cggtgtcggt gggcacggac
    15901 gcgtcggagt tgctctcgcg cagctctcgc gccaggcgct ccgcctcgag gacgagggcg
    15961 ccgtaggtag gccaggcccg agggtcgtcc agcggagtca cccgcacctg ctcgctgagg
    16021 tcgtcgaggg tgtccagggc gcgctgcacg tgctgctcca cggcctcacg ggacggctcg
    16081 gtccacaccc cgaagtcgga gatcgcgtcg ccgatctcgt cgagcacccc ggcgtagtcg
    16141 tcgagccatc ccgccgacgg tgcgggatgc cgggccgcct cgtccgcggc gtcgacgagg
    16201 gtgcgcgcga tgctgcccac ctgccactgg gcccgccgaa cggcctccac cgtgcgcgtg
    16261 tagccgtccc agtcgatgcg cgccgggcgc aggcggtgcc gccagttgaa ccgggtgctc
    16321 tcccgcccgg tctccacgac gccgagcacc tcgggaaccc ggtcgccgag gtcggtcgcg
    16381 gtgtcgtacc accgccgggc ccggtcggcg tcgaagccct cgcgcaggcc cgacgccatg
    16441 tccttgagca ggtcctgcac ccgggtggtc aggtcgcgca gcgcgtcacg cggctcgtcc
    16501 aggtgcatgg gagcgagcac gaccgcattg acggcgacac cgacgatgcc gccgagcacg
    16561 gtctccacga tggtgaggta ggtgaagtcg gtgtcggtgc ccttgaccgt gagcagcgac
    16621 agcagcgcca tggtcggcac ctggatgccg tggtccccga ggagaggcca gcgtgcgatg
    16681 aggagcgcgg ccatcatcac cgggaccatc gaccaccacg tcaccccgac gagcgacccg
    16741 acgagccagg cgatgctcat cccgaggacg acggcggcca cgcgccgggc gctggccccg
    16801 atcgagcgga ccatggtgcg gtcgacgacg agcagggcag ccatcggggc gtagaagggg
    16861 gtggggctgt cgagcaggtg cacggcgagc tgccaggcca gcacggtcgc gaccgccgcc
    16921 ttgagcatga gcaggccctc ggcccgctcc acgccgggcg tggtcagggc agcccgaacg
    16981 gccgaggccc accccccgag ggtcgcgcga acccggctcg gcagtccgcg cggctcgacg
    17041 agctcatcct cacggctgac caccacccga ccatagcgag gcggcggcct ccgagacgag
    17101 ggtcaaccga acgcggcccg gtctgctcag acctcgaggg tcagcacgag gtgcgtggtc
    17161 tccggcgact tcgccgagcg cagggcggcg accgtgcgtg ccggctcgcg gtcgacgacg
    17221 agctcgaccg tgctcggcag gaagaccggc ttggtgaacc acacgtggct cgtcgacggc
    17281 ccgagcgagg tgcgaccgag tgcggcgagg gtgcgggcgc aggtccacat gccgtgggcg
    17341 atgtgccgcg ggaaccccat ggccttggcg gtcaggggat gcaggtggat cgggttcacg
    17401 tcacccgaca ccgacgcgta ggaccggccg aggtcctcgg gcaggcgcca ctgcgccacg
    17461 ggctggcccc cgggcggcgg cgggggcccg tcaccgcgcg gggcgtcctg cgtgccgcgg
    17521 ccacggctga ggtagacgct gtcgcactcc cacacgagct cgccgcccac gcgtgcctcg
    17581 agccggacgt cgagcgttcg cccgcgtcgg tgcgggcgga tctgcccggc cgacaccgac
    17641 agcgtgagcg ggtcaccggc atccatcgcc cggtggctgg tgacccggtt ctccaggtgc
    17701 accatgccgg gaaggggcat cggaaagtcc ggccgagcca tcaaggcggt ctgcaccggg
    17761 aacgcgagca cccacgggta gggctgcgga acggtgtcgt cgacggcgta gccggtgagc
    17821 cgctggtagg cagccagctt ggcccggtcg atccggacgt cctccacgac gagcgtgcgg
    17881 tcgggcaccg cgtcgccgcg ccgcccgcgt gacgtcaggg cggcacgaac gagcagtggt
    17941 gccgtggacg gtacccccga caggatctcg gtcgccacgt caggcaccga gcaggctctg
    18001 cccgcacacg cggacgacgt tgccgttgac gccggactgc gcgtcggagg cgaagtgggc
    18061 gatcgtctcg gcgacgtcga tcggcagacc gccctgcgcg aggctgttca ggcgccggcc
    18121 gagctcgcgg gtcgcgaacg ggatccgtgc cgtcatctcg gtctcgatga accccggcgc
    18181 gacggcgttg gcggtgatcc cccgcttgcg cagccccttg tcggcggcgt acgcgtcaac
    18241 cagtccgatg acccccgcct tggaggccgc gtagttcgcc tggccgcggt tgccggcgat
    18301 gcccgcgatc gacgagacga gcacgacgtg gccgccgtcg cgcaggccgt ccctggcgag
    18361 gaactgctcg ttcatcgcca ggatcgacgt gaggttgacg gccagcaccg aaccccaccg
    18421 ctcggcgtcg gtgttcgcga gcagcttgtc gcgggtgatt ccggcgttgt gcacgacgat
    18481 gtcgagcccg ccgtgccggg tgcgggcgtg gttcatgatg cgcgcccccg cgtcgggtga
    18541 cgtgacgtcg gcctggagag ccgacccccc gatcgagttg gcgaccttgg ccagggaggc
    18601 cccggcgctc ggcatgtcga cgcacacgac cctcgccccg tcacgggcga gcgtgcgggc
    18661 gatgtccgca ccgatcccac gagccgcacc ggtgacgacg gcgacctttc cggccagggg
    18721 tgccgcggca tccgcgggtt cctgcgacgt ggccacgccg atgcggaaga cctgaccgtc
    18781 gacgtaggcc gagcgggccg agagcaggaa caggaccgtc ccgagggcgg caccctccga
    18841 ccccggcgtg aggagcacga ggttcgcggt cgcccccgcg cgcagctcct tcgcgaccga
    18901 gcgggtgatg ccttccaggg cgcggtgggt ggcccgctcc gcggcggagc cggtggatgc
    18961 cgggtcggca ccgaggatga cgacgcgcgc acagcgcccg agtcgcttca gggccggggc
    19021 gagcgtggcg cgcagcgact cgaggtcggt gggggtggtg gccgccgaca cgtcgacgac
    19081 gacgccgccc agtcgcccct cctgcgggac ctcgtcgacg atcagcgcac ctgcctgccc
    19141 gagctttgcg cgcatcgcag ccagggctgg gatcgacccg tgaccaccga cgaggagcgg
    19201 cccgtcgacg agtgggcgcc cggcgacgtg gcggcgcagg gcaaccggct ggggcaggcc
    19261 gagcgtggtc gagatcttct tgccgacgcc ggaggagacg aatgccgtgt agccgtcgcg
    19321 gtgtcccatg tcaggccgcc tcgaggatcg cgacgacgcc ctgcccgcca gcggcgcaga
    19381 tcgagatcag gccgcgggat ccgctgccct tctcgtgcag gagcttggcc agcgtggcga
    19441 cgatgcggcc gccggtcgcg gcgaaggggt ggccggccgc cagcgacgag ccggcgacgt
    19501 tcaggcgcga gcggtcgatc gcacccagcg gggcatgcag accgagccgg tcgcgacaga
    19561 agtcgtcgtc ctcccaggcg gccagggtcg acaggacggt cgcggcgaag gcctcgtgga
    19621 tctcgtagaa gtcgaagtcc tgaaggcgca gcccctgccg ggcgagcagg cgcggcaccg
    19681 cgtaggaggg cgccatgagc agcccctcgt caccgccgac gtagtcgact gcagccgtct
    19741 cgccgtcgac gaaccacgcg agcggcttca ggccgtgggc gtcggcccac tcctgcgagc
    19801 cgaggaggac ggcggacgcg ccgtcggtga gcggcgtgga gttgccggcc gtcatggtgg
    19861 caccctcccc cttgccgaag acgggcttga gcgacccgag cttttcgagg gtggacccgg
    19921 ggcgcaggtt ctggtcgacg gtcaggccga ggtacggggt gaggaggtcg tcgaagaagc
    19981 cgcgctcgta ggcggcggcg aggttgacgt ggctggccag ggccagctcg tcctgggcct
    20041 cacgggtgac cccccactgc gcagcggtca gcgccatgtg gtcacccatc gacagccccg
    20101 tgcggggctc ggtgttcgcc ggcaccgaca cgcccacgtc ggtcggacgg aacgccaggg
    20161 ccgccttcac gcggtcggcc gaggtcttgg cacggttcag acgcagcagc ttgcggcgca
    20221 gcgactcggt gacgacgacg ggggcgtcgg aggcggagtc ggccccaccc gcgatgcccg
    20281 actcgatctg accgagggcg atcttgttcg ccacgaggat ggcggcctcg agcccggtgc
    20341 cgcaggcctg ggtgatgtcg taggcggggg tggtcggcga caggctggag ccgaggacgg
    20401 cctcacgggt gaggttgaag tcccggctgt gcttgatgac cgcgcccgcg acgacctctc
    20461 cgaggcgttc cccggcgagg ccgtggcggg cgacgaggcc ctcgatggcc gcggtgagca
    20521 tgtcctggtt cgcggcctcc gcgtaggcgc cgaactggcg ggcgaacggg gtgcggttgc
    20581 cgccgaggac ggcggcgcgg cggggggtgg tcaccggaag ggctccttgt gcgtcggcag
    20641 atgtatccgg tacggaaggt aacaggtact gagggtttct gctagcgtcc gcgcatggat
    20701 gtgacgcagg caccaccgga ccggcgccgg tcgcgctggg acgagcaccg cctcgtccgc
    20761 cgcgccgagc tcgtcgaggc aaccctgctc gccatccgca cccacggcgc cggggtcggg
    20821 atggacgagg tcgccgccac cgcccgcacg tccaagaccg tgttctaccg gcacttcacc
    20881 gaccgcgccg gcctctacac ggcggtcgca gagcgggtcg acgccacgat catccgcgac
    20941 ctgacgaggg ccgccgccga cccggcatcc gacggctcgc gcgacggcgg acgcgccgtc
    21001 atccgagggg tcatcgccgc gtacctgcac ctcgtcgagg acgacccgca ggtctaccgc
    21061 ttcatcgtca acgcgccgat ggtccccgcc ggtgaacggc ccgagggtga cgtcgcggcg
    21121 ggcatgaccg ggcggatcgc cacgcacgtc gccgatctcg tcgcaggggg cctcgccagt
    21181 ggttccggcc gtccatcagg ttcggccgcg ccgcctgtac ctgccctcga cgcgcgggcg
    21241 tgtcagctct ggggggtggc gctcgtcggc atggtccgag ccgtggcgga ctcctggatg
    21301 gccgagggtg gcgcccggaa cggccccagc agcgacgagc tcgccgacca gctcaccgcc
    21361 ctggtctggg aaggcctgcc cgacgtcgcg gcaggcgccc agcacggctc aggaaggtcc
    21421 tgagcagccg tggtctgagg agctgggcgg tgggacgttg gccgcggcgt cgatgacctc
    21481 ctcgagggca gtgcgcggtg gcgtagggcg tgatcgcgtt ccgcgacgcg gttgcagcat
    21541 tccgggcgta gctgacctcg gcccctgcgc gccggttgac gacgtgctcc agacacaaga
    21601 gccccgaacc gccggcgcgg tcggggcatt tacgtggatg tggagggact cgaacccccg
    21661 gcctctcgcg tgtgaagcga acgctctaac caactgagct acacatccgg atgccgtgaa
    21721 cggcgtgccc gaggatatac ggccaccgcg cccggcggaa atcggccccc cgtcacaggc
    21781 ccggaacgtt gccctcgagc caggtcacca tcgacgtcgg gaggaagccg gggacaccgc
    21841 tgaccaccag gagcgcccac aggacacaac cgacgaacgc cgccgtcgcc agaccgacga
    21901 gccccttgat ccacagcggt tgccgccgca cccactgctc ccacgcccgc accttggcct
    21961 tgacgaagtc gagcagccgc gctgcctgct cgaactccga ggcccagatc gcgatgccgg
    22021 cgatgacgat cagccatccc ggcccgggca ggggaaccag cgcgagacca ccgagcagca
    22081 ggacgagccc gacgacgaac acggcgatgc ggtagatgcg acgcgacgtg gggtttgcgc
    22141 ggatggcgcg acgccaggcc cagtcgtcct cgtcattcgc atcacgcagg acgtgccggc
    22201 gctcctccgg cgtcacggat cgatccccct ggcggcgtgc cgggcccaca cctcacgtgc
    22261 cgctgaggtg accggccccg gcgccccgag ctcacgaccg tcgaccgccg acaccgggaa
    22321 gacgtccttg atcgacgagg tgaggaacac ctcgtcggcg tgctccagca cgtcgagggg
    22381 catcgcctcc tcgacgatgt cgagcccgtc ctcacgacac cactcgagca cgagttcacg
    22441 ggtgatgccc gcaaggcatc cgctgttcag tggtggggtg cgcagggtgc cgtcgagcac
    22501 gacgaagacg ttgctgcccg tggcctcgca cagctcaccg cgcgtgttgg cgaagatcgc
    22561 ctcgacggcc ccgcgctcct tggcgtaggc gagggcgatg acgttgtcgg cgtacgacgt
    22621 cgtcttcaga cccgcggtgg cagcccgctc gttgcgcacc cacggcacgg tgaccaccga
    22681 cccctgcgcc tccggacgct cgtgcgcgac ggcggtgacg atgtgcgtga gtgccgagtc
    22741 gagtcgatcc gaacccaagg gacccttgcc gccggtcacc gtgaagcgca accgaccgaa
    22801 gtcgatcacc tcaccgtcga ggacggcgcg gatgccgtcg tggatctgtg cgtggtcggc
    22861 cggggggagc ccgagaccgg ccatcgtgcg gtcgaggcgg cgcaggtggc gcgtgacggc
    22921 gaacggtgcg ccacggtcga tcttggcggt ctcgaaggcc ccgtcgccca ccgtcacccc
    22981 gtggtcgacc gcgctcaccg cgggggtgtc agcgtcgacg agctccccgt tgacccagat
    23041 cctgatgtca gtcatgcgtc acactttcgc acgcgcaggt gcctcccctt cggcgagggc
    23101 gacgagacgc gccgccttga gctccgtctc ggcccattca ccctgtgcgt cgctccccca
    23161 cgtgatgccg gcgcctgtgc cgaatctcag gactcgtcgg ccgtcaccat cgcgggcgac
    23221 ccagaaggtg cggatgccga ccgcgagctc ggcctcgtcg cggtcgatgt cgatccaccc
    23281 cacggcgccg cagtaggggc ctcggccgac cggttcgaga tcagcgatcg cgagcagcgc
    23341 cgtgtgcttc ggtgccccgc tcacagaccc cggtgggaag ctcgcgtcga gcaggtccct
    23401 ccacgaggtg cccggccgca accggcccga cacgtccgag accaggtgca cgagaccggg
    23461 gtgctcctcg acccggcaca ggtggtcgac ggagacggtg cccggtcggc tgacgtggcc
    23521 cacgtcgtgg cgcacgaggt cgacgatcat gacgttctcg gcgtagtcct tcgcaagcag
    23581 cagctcaggg gtcgtcgcag tgcccttgat gggccgcgac gtcaccaggt cgccctgtcg
    23641 cagtaggtag gcctccggag aggcgcaggc gagctcgaga cccgcctcgg gcagggcgat
    23701 ggtcgccgag tacggcgcgg ggttccccgc ggcgaggcgg tcaccgagca ccgcgatgtc
    23761 ggccccctcg tcgagatcgt gctcgagcac gcggcagaga ttgacctggt agaccgtccc
    23821 ggcctcgatg cgcccccgaa tctgcgagac gccctcgaca tacgccgtcc ggtccaggct
    23881 cgtgcgccac gggcccgaca gcgccggcca cgcaccgaca tccgagcgtc cttccctgcg
    23941 tcgtacaggc tcggtgcggt cgacgtcgtc catccgcacg gcggtgagct caccctcgaa
    24001 ggtgatcacc accacccaga agccgctcgt gaggtcgtcg atgtcgtggc tgacggcgct
    24061 ggcaccgcgg gccacgagcg acgtgaacct cgcctccgac acgcccgcat cgtatgccgt
    24121 gtgccgttgc ctcacccggt gctcacgtcc agatcggccc gcccgagggt ggcccggatg
    24181 acggcggcgt tgggttcatc gacgctcgcc acccaggcag cggcctcggc tggtgtcctg
    24241 ccgtaccgca cgtggcgggc gatgagccgc tcggtgcgaa tcgccgcatc gacccgcacg
    24301 gcccacacct cgtcgagcag ctcccccacc tgcgcccacg gcccctcgtc gacgaggagg
    24361 tagttgcctt cggagacgac gacctcggtg ccaggtggca cggggatcgc acccgcgatg
    24421 gactcctcca ccgaccggtc gtacgtcggc gcccacaccg tgtccgggcc ctcggcgcga
    24481 atcctcgcca acagggctgc gaagccggcg gcgtcgaagg tctcgagggc gcccttgcgc
    24541 tccgccagac cgagggaggt cagcgcgacg ttcgcgaggt ggaacccgtc catcggcagg
    24601 atcaccgcgg cgcagccccg atccgtgagt gcggcgacga cggatgccgc cagcgtggac
    24661 ttgcccgccc caggctcacc ggcgataccg atgatgacca cccggccagc cgtggcggcg
    24721 cgcagcgcga gaacacggtc gaccaggtgc aggtgatcga cgacctcggc gtggtccgtc
    24781 atcggcagtc ctccgcgtca gcgatgacga ccgacgctag cccataccgc ggcgggacgc
    24841 tgcccacgtg aaggcctgac ggacggcgga acggcgcctg cctttcgccc tgggaacact
    24901 cagacggtcg tcggcaccga ggccgcgacc gcggctcccc agacccgggc atgctcgagt
    24961 tcgccatcgg cgaggggccc ttcgacgtcg agcacgtaga agctctgcgg ggcgatcgcc
    25021 tgcgagaacc cgtgccggcg gccggcgcgg gccgcgctgt gggctgccga ccctggcagg
    25081 cggcgcacct tgctcaccct ggtgtcgaag gccgcgaacc atcggccgtg ctcgtccggc
    25141 aggctcgcga gccactcgcg cagtccggtc gccaccgagc cctgcgacgc cccttggact
    25201 cgggcatcgc gcctcgtcga ctccctgctc atggagaacg cgtgggtcgg gccaccggcg
    25261 acgatgaggt cgaccccctc gagctccgtc ggtgccacag tcacctcgac gagcttgacg
    25321 ggcatgacct cggcgacacc ctgcgcgacc gctcgcgcga ccttctcgga gttgccgaac
    25381 atcgactcgt agaccaccag tgcccgtgcc atcgtgcgtg ccttcccggc ccgggcgacg
    25441 agcccgaagg gccgcctcca cgctacgccg gaggtgaccc gccggacggc tgcaccggtg
    25501 tctcgtcggt cacggttggc gtccgcggtc acggttggcg tccgcggtca cgg
//
LOCUS       DAOSOC010000018        25252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1970_length_25252_cov_9.279636, whole genome shotgun sequence.
ACCESSION   DAOSOC010000018 DAOSOC010000000
VERSION     DAOSOC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 25252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25252
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1970_length_25252_cov_9.279636"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PL014_02770"
     CDS             complement(<1..627)
                     /locus_tag="PL014_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT56048.1"
                     /translation="MFIDLHTHSTASDGTEPPAVVVAQAVAAGLDVVALTDHDTTLGW
                     EDAAGAARRHGIRLVPGIEVSCSRHHQSIHLLAYLPDPTYPDLVAELERALHSRDTRL
                     DAMVAKMAADGIPVTIEEVRAQVEPGATPGRPHIADALVASGVIGHRDEAFARWLGGR
                     SPYYVEHYAPDPVRAVEIVRAAGGVPVIAHPRSVNRGRVVSDALVEELA"
     gene            724..1239
                     /locus_tag="PL014_02775"
     CDS             724..1239
                     /locus_tag="PL014_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HOT56049.1"
                     /translation="MGSATKSERDAAFTAFVHQASPSLLRTAWLLTGDHHTAHDLVQA
                     ALVRTYVAWPRVRADSALAYTRRVLVNERTDRWRRHGAEVALAAVPETTSPGPESVTD
                     ERDAVVRLLARLPDQQRRVVVLRYYVDLSEQATADALNISVGSVKSAASRGLATLRAE
                     LASTTSEGVVR"
     gene            1236..2312
                     /locus_tag="PL014_02780"
     CDS             1236..2312
                     /locus_tag="PL014_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56050.1"
                     /translation="MSTDTFEDTLRDLLRDTADAAGPGCLEPDADRVIGLGRRVLRRR
                     RWAAGLVAASVAGVAGVMGVTALGPGADESSLPAGTTSATTSGVGEVTADLTRSGSVE
                     NAWGPADEQPSVEVAVDMGRRTLSSSTLGQDGRTSDVRSATLPTNPRASMWVPTTTPG
                     LERGVLPAAAKDVIFLWAGDQGAGTHDLQPLPGTPYQAFAVWVGGENGGPPPLSGVLW
                     TDGAAVFRTDGSEVHSARDGAALAFVDRDQGVLGIFDDGSSSVAALPERGIPTIMTGS
                     QMDGTDTLETTVLLVLPPGVDEVGVTAAEGATLRSSETHRGSSTQDTLVVAHLSVTSP
                     AAGTGVDSVTWTNADGSPGGGPVS"
     gene            complement(2339..2875)
                     /locus_tag="PL014_02785"
     CDS             complement(2339..2875)
                     /locus_tag="PL014_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOT56051.1"
                     /translation="MQFGRSYEEFEVGATYKHWPGKTVTEYDDHLFCLLTMNHHPLHL
                     DTNYAQETTQFGQNVVVGNYVYSILLGMSVPDISGKAIANLEIESLRHVAPTFHGDTL
                     YGETTVLDKWESTSKDDRGVVHVETIGYKQDGTIVCIFRRKVMVPKENYLQARGGEQP
                     GRPTPVPDKNWPGPASPA"
     gene            complement(3011..3955)
                     /locus_tag="PL014_02790"
     CDS             complement(3011..3955)
                     /locus_tag="PL014_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56052.1"
                     /translation="MDDRLTAVAGSRGGVFTSAMAAVVDVDDGALARGRRSQEVVRVR
                     RDAYVLGELWSAATPEQRLMLRTRAVLAARPEDIASHQSALALHGLPLHGVDLVTVDV
                     LSAVSRVRLASGLRTHARPADQPHVVADGYRCVDIATGIAQVLVRSGVGAAMVPLDAA
                     LHAGQCTLGEMALALDDRCSSRRLRARGDQLLSRADPQSESVGETRTRLMLTDHGLAV
                     RSQVPICDASGTLVGRVDFLVGDHVIVEFDGAVKYEGAQGRSALVAEKRREDALRALG
                     YVVVRVTWSDLASPTRVISQIRRALAGLSTRDGHVFTP"
     gene            4120..5637
                     /gene="glpK"
                     /locus_tag="PL014_02795"
     CDS             4120..5637
                     /gene="glpK"
                     /locus_tag="PL014_02795"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOT56053.1"
                     /translation="MADFIGAVDQGTTSTRFIVFDHDGMPVASHQLEHDQILPRAGWV
                     EHNPLEIWERTQTVIGSALTSAGLATSDLAAIGVTNQRETAIVWDRRTGRPWYNAVVW
                     QDTRTDSIAKALDADGRGDVIRRKAGLPPATYFSAGKVQWLLENVEGLRAAAERGDAL
                     FGTPDTWVVWNLTGGPDGGIHATDVTNASRTMLMDLTTLDWDDELLGFFGIPRAMLPS
                     IHSSSDAAAYGVTTGDRSGAAGVRITGVLGDQQAATVGQVCFEPGEAKNTYGTGNFLL
                     LNTGEELVRSSNGLLSTVAYRFGDAKPVYALEGSIAVTGSAVQWLRDQLGIISGASEV
                     ESLAASVPDTGGVYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHVARATLEAICYQ
                     SRDVVDAMAADSGVTLTTLKVDGGITANNLCMQLQANILGVPVSKPVVPETTALGAAY
                     AAGLATDFWSGTDELRANWAQARRWEPQWSDAQRRDGYAGWQKAVERTLGWVDVE"
     gene            complement(5639..6661)
                     /locus_tag="PL014_02800"
     CDS             complement(5639..6661)
                     /locus_tag="PL014_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT56054.1"
                     /translation="MTSPQRPRDPRRADIRRSRRPSPEVIARRRLVVGGGALLGVGVL
                     GLGVRELFTDDAVASPAMVTTAGPTTVDGSAGEPTGATASPSPSATASASGVPGGQAS
                     PVEAPKLANRLEEYLSGRSGRLGLEIVDLRRGQSFRHEASEGYCYSTIKVLVLTTVLR
                     QAQEAEVELTERERDLATRMITRSDNGATETLLAQVGRDEVERVAGLVGMTNTQIDSG
                     WWGFWRTVPGDLSLMVDAVLSSDRVLDGGRRTIARMLMADVVPQQRWGVFAPESRKVH
                     VAAKNGWGPLPDGYRLNSTGWVSGADRDYVLSILSRSTAGFSHGRRTVNEVADICHSA
                     MADGLA"
     gene            complement(6658..7704)
                     /locus_tag="PL014_02805"
     CDS             complement(6658..7704)
                     /locus_tag="PL014_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HOT56055.1"
                     /translation="MGQGVVDVAGETLADRFRAHFGPEDHLYAALLARMADDWEAGGV
                     TREIFDGWQDAPAQLMPHLRMLAGLFRIVLRGEAPQLEGFYPALGGDLDPRDAWDAVR
                     PVLLARRDELRSSLAVAPQTNEPARTLALLIGVSDAVHRSGLNRVRLLEPGASGGLGL
                     LVDRYRFVGEGWSAGPVEATLVVPGCGAEGFRPESFEVVERRGCDLAPVDTSSAEGGE
                     YLTSFVWPWQRERHARLTAALTVAREHPVVVDRAPASQWVAERLAEPVPDGVLTVVWH
                     SVTRMYWPAEETAAVDDAVRVARGRMPLAHVAFEHDWAAASGSGSPGLPRVELDGEVL
                     GLGDHHGPPVRWSR"
     gene            7759..9264
                     /locus_tag="PL014_02810"
     CDS             7759..9264
                     /locus_tag="PL014_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOT56056.1"
                     /translation="MSEEQQQADNRARATTDEFRAFVRDGWAPRRPTVTGPSPAAAPA
                     AAHRALISAAYPRQRLIIPAGGLKVRSNDCDYVFRPHTAFAWLTGLGADREPDAVLVL
                     EPVAGADGTDGGHEAVLYFRPLADRDSDEFFADSRYGEFWVGARPSIADIELELGLTA
                     RHIDEFPAAASKDAGEVTVRVVRDADLALTAQLDAARVENGAEQEGLKESDAALAHDI
                     SHLRMCKDDYEIEQMRAAVAATHHGFDAMVAELATAATVERGERWLEGTFALHARHRG
                     NGVGYDSICAAGDHANTLHWIKNTGEVREGDLVLIDAGVEVDSLFTADVTRTFPVSGT
                     FTDAQREIYDAVYAAQEAGIAAVKPGNKFSDVHAAAIRVIAEHLHAWGLLPDGVSVED
                     TLDKEHGQYHRRWMVHGTSHHLGLDVHDCALATREEYMDAELRPGMVLTVEPGLYFKS
                     DDLLAPERFRGTGVRIEDDILVTQDGCENLSAGMPRSSADVEAWIARLRTS"
     gene            complement(9372..10112)
                     /locus_tag="PL014_02815"
     CDS             complement(9372..10112)
                     /locus_tag="PL014_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT56057.1"
                     /translation="MTDARPHLEPFEGEFARVLCVVAHPDDIEYGTAAAVHRWVAEGA
                     TVSYFLLTRGEAGIDTMHPDEAAVVREAEERESARRVGVDVVEFGGFADGAIEYGVAL
                     RCAIARAIRRERPDLVVTTTWDERFGTGMVNQADHRAVGLATLDAVADAGNRWLHTSL
                     AEEGLEPWGGVKALAVAASSSPTHFVDVSGEHFEAAVHSLEAHAAYNDALPEDFPKPR
                     ALLQMVLGGGGQAAGVEHAALFDVIRRG"
     gene            complement(10122..10676)
                     /locus_tag="PL014_02820"
     CDS             complement(10122..10676)
                     /locus_tag="PL014_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56058.1"
                     /translation="MTSQPMRPGLRAALSLQYPMSLGVYDSYEEAQKAVDYLSDNEFP
                     VQDVLIVGTDLKQLERVTGRLTRGRVLGAGALSGAWLGLFVGTIFALFDPSGFNILNV
                     VSTVAFGALFGMVWAAVGYRLTRGERDFTSVSQVVAAKYEVLCEHKHVQRGRELLTEM
                     DPMRAAMQEVRRAQEAERARADEA"
     gene            complement(10751..11947)
                     /locus_tag="PL014_02825"
     CDS             complement(10751..11947)
                     /locus_tag="PL014_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT56059.1"
                     /translation="MSRLQTTDGLSEEQHELIKLVREFVEEQILPVATELEHRDEYPT
                     DIVEGMKEMGIFGLMIPEEYGGLGESLLTYALVVEEIARGWMSVSGIINTHFIVAYLI
                     LQHGTEEQKQRYLPRMATGEVRGAFSMSEPGLGSDVAAIRTKAVREGEEWIVTGQKMW
                     LTNGGSSNLVAVLVRTDLGESESPYKNMSTFLIEKESGFGETAQGVTVPGKIEKMGYK
                     GVDTTELIFEGHRTTDAQLLGGVPGKGFYQMMDGVEVGRVNVAARACGLSRRAFELGI
                     AYSQQRETFGKKIAEHQGILFRLADMATKVEASHQMMVKAAKLKDAGERNDLEAGMAK
                     YLASENCADVVEQSFRIHGGYGYSKEYEIERLYREAPMLLIGEGTAEIQKMIIGRRLL
                     EDYKAR"
     gene            12065..13459
                     /locus_tag="PL014_02830"
     CDS             12065..13459
                     /locus_tag="PL014_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOT56060.1"
                     /translation="MAARAPAHPHGGHPPTVEGRLRPALALRRNVVEFAQRSWQEHGD
                     VVRMVVGPRGLDREMWWLHHPDAAAKVLSGASWRSFAKRDQVYDEIATWLGRGLLAAE
                     GEDWTRQRRVLQPVFTHAAVNDYTDLMVEEMESVISGWPVAAAPTPVDLGERMQHLTL
                     RVVLRALFGESASDATQRVRRSFPALSAAIISRGLTPLRLPAAIPTAGVRRARAARAD
                     LVDLVDAMVEARRSGRTTGGDDLLGHLLTARDGERLLSDGEVRAQALVFLLAGHETTS
                     SALTYTLHLLGRHPDVQDRLRTEVHDVLGERRPSATDAASLPETTAALKEAMRLYPSA
                     PVLGRLAVEDDEIMGYPVAAGTSVVVAPWTIHRHPDFWVDPLIYDPSRFTTNGPRPQP
                     AHRYAWMPFGGGPRACIGQHFSMVESVLALALILRSHRVAPTRSTDADHLPVGAHLTL
                     VPTTPVLATLSRLP"
     gene            13651..14505
                     /locus_tag="PL014_02835"
     CDS             13651..14505
                     /locus_tag="PL014_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOT56061.1"
                     /translation="MAHQPFHLPAKQELFATDTSPDAPTEGERWSTWDGATHGPKPRP
                     SWVITDLGAVEADLGVLKTGKEADVHVLRRWVPGTERSSTMAAKRYRSGEHRLFHRDA
                     GYLEGRRVRKSREMRAMATRTEFGKQLISGQWAAAEFSALGTLWALGLPVPYPVQLDE
                     TEMLMEFVGDVSGPTPVAAPRLVATRPDPDLLADLFEQLRTAVLTLAHHGWAHGDLSP
                     YNVLLHDERLVIIDWPQIVDVIGNPQGFDFLARDATTMATWFTRRGLDVDAGEFVGDL
                     VAEATSRF"
     gene            14534..15583
                     /locus_tag="PL014_02840"
     CDS             14534..15583
                     /locus_tag="PL014_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT56062.1"
                     /translation="MSSAPAAPATLAASTVPDPMDAPAIRWGVLAPGGIANTFADAVA
                     TGTRSRVVACGSRSLDRAREFAQRHGVERAHGSYADLVADPDVDAVYVASPHSEHHEH
                     ALLALRAGKPVLVEKAFTRSLREAEHVFDAAQAGGLLTAEAMWSRYLPHYDVIARTVA
                     AGTLGDVVVVEADHGQLLYPDGPARLSQPELAGGALLDLGVYPVSFADHVLGSLADVT
                     ARGVLTDLGVDATTTIDGRGPSGELARLWCSMAAATACPARVVGTAARLEISGVFYAV
                     DSRVRLVAPDDTVLDEFVAPVLDHGFRYQAAEFARALAAGRTQTWSMPWDATRRVMAT
                     MDETRRQVGVVYPGE"
     gene            complement(15623..16183)
                     /locus_tag="PL014_02845"
     CDS             complement(15623..16183)
                     /locus_tag="PL014_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOT56063.1"
                     /translation="MLATQQRLTELGYWLGTPDGHFGALTQQAVFALQKAAGLSRDGS
                     VGPKTAKALADGIRPRATLSGNGVEIVLDRQLLLVVRGGTVRTILNTSTGNREEYTST
                     SGNRAIAVTPKGTFKVYRGVDGPLTNSLGQLWRPRFFYRGIAVHGSPNIPPWPASHGC
                     ARLSNAAINMIWDKNLMPIGSTVVVR"
     gene            16224..16601
                     /locus_tag="PL014_02850"
     CDS             16224..16601
                     /locus_tag="PL014_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56064.1"
                     /translation="MAVGDGEGLDFADGFAEGLGDGLGAFVVLGDGFGAALVVGEGVA
                     DDALCVTVGTGVDAVGALTDGAALDTDGDGVSGATDGGAALVVLCCGAAASEDQDAPS
                     PAHPPRTSAPARARPGVAARTLR"
     gene            complement(16713..17585)
                     /locus_tag="PL014_02855"
     CDS             complement(16713..17585)
                     /locus_tag="PL014_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOT56065.1"
                     /translation="MSAATDYRPRRSVLYMPSSNERALEKAKTLPVDGLILDLEDAVA
                     PDHKATARDNACAAARSGDYGQREVTIRVNASGTQWHDDDLAAACAAGPDGIVVPKVD
                     SAQEVLDLVAAMERHGAPEHTRLWAMIESPAAVLAVGEIAAASPRLAALVIGTNDLVK
                     ELGARHVPGREPLLTSLSLALLAARRSGVAILDGVYNDVTDLDGFFAECRQGADMGFD
                     GKTLIHPGQVGPCNEVFAPSPAEIEDAHALVAAWDAGAGSGVVTHRGRMVEHLHVEIA
                     RQVIATAQAIADRA"
     gene            complement(17582..18667)
                     /locus_tag="PL014_02860"
     CDS             complement(17582..18667)
                     /locus_tag="PL014_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOT56066.1"
                     /translation="MRSAKDFFTPLAVGAPVPVREVPARPSRMIHFFDPSNPRMAAKV
                     PQLVGTVDVLLGNLEDAVKAENKDAARAGLVEIARATDFGEHTQLWTRINSLDSPWVL
                     DDLTTLVTEVGDRLDVVMVPKVQGPEDIHYVDRLLAQLEAKAGLTRPILVHAILETAR
                     GVANVEQIAGASPRMQGISLGPADLAADRRMKTTRVGGGHPGYLVRADPVGGDITGSR
                     PTFQQDLWHYTIARMVDACAMHGIFPYYGPFGDIADVVACEDQFRNAFLLGCVGTWTL
                     HPAQIAIAKTVFSPSAQDVAHARRVKEAMGDGTGAVMLDGKMEDDASLKQCLVILELA
                     DRLSANDPELAALYAGAAGTTDAGAQA"
     gene            18762..19334
                     /locus_tag="PL014_02865"
     CDS             18762..19334
                     /locus_tag="PL014_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56067.1"
                     /translation="MTDDASTQVTPGAPTINVTRLIHEAASKSGLLWIRVPGAGTHPV
                     WHVWHDDGDERGTGPAAYVVSGPGEQSLPWLPDEVELVLRSKDSGGLLLSVRASSREL
                     TPADEDWDAAVQVLRPERLNAVGDVAQRWAQGCTIHVLTPHGRLVHGPGAYPSDSGSM
                     SVPSGPGTTARWRPWHWRGRAGARRNTPPA"
     gene            19372..20667
                     /locus_tag="PL014_02870"
     CDS             19372..20667
                     /locus_tag="PL014_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT56068.1"
                     /translation="MSTPTRAFVGRLTELSVFDPLGDQVGRVRDVVVTFNVARTRPRV
                     IGLVVEVAGRRRVFVPMTRVTSVEGGQVITTGLVNMRRFEQRGNETLVVAELFERPVE
                     VRTEEGGLLAATVEDVGIEQHGSRGWSVARAFVRTGAPRGGLGRLARRRGETRLVAIE
                     DVVGLRTSSSAQSAARLVEAYEDLKPADLAEVIHDLTPQRRAEVAAELSDERLADVIE
                     ELPEDDQVEIIAGLRVDRAADVLEAMEPDDAADLLAELPKDRQEELLERMDADEAADL
                     RRLLTYDENTAGGLMTTEPVILGPEASIAEALAVVRRQELSTALACTVFVCRSPLETP
                     TGRYLGMVHIQRLLREPPHNPVGGILDKDVEPLLPDATLGQVTRMLATYDLVGVPVVD
                     AEGHLLGAVTVDDVLDHILPDDWREDRHDSVTPEGATRG"
     gene            20660..21208
                     /locus_tag="PL014_02875"
     CDS             20660..21208
                     /locus_tag="PL014_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOT56069.1"
                     /translation="MAERERRPRIDQPREGGRRFTVPSVFTEEAFGVVSEKFARYMGT
                     PYFLIGMTLFVLIWVGWNVLAPPSQRFDKYPFIFLTLMLSLQASYAAPLILLAQNRQA
                     DRDRFALEQDRSRDERNLADTEFLTREVAALRIAMRDQATRDFVRGELRDLLDEMEER
                     GLVVVRAPESDEAPVRSPGSPT"
     gene            21219..>22310
                     /locus_tag="PL014_02880"
     CDS             21219..>22310
                     /locus_tag="PL014_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOT56070.1"
                     /translation="MSPVTPDALHAALETVIDPEIRKPVTELGMVESVSADESGHVAV
                     TILLTVAACPLKDTLTRDTTAALEKVEGVTGVTVTLGVMSDEQRTELKTKLRGGVAER
                     EVPFAKAGSLTRVYAVASGKGGVGKSSVTANLAAALAEQGLRVGVVDADIYGFSIPRM
                     LGVEGRPTQVDDMILPPLAHDVKVISIGMFVPGNQPVVWRGPMLHRALQQFLADVFWG
                     DLDVLLLDLPPGTGDIAISTAQLVPGAEILVVTTPQQAAAEVAERAGAIALQTHQRVA
                     GVIENMSWLELPDGTRQEIFGSGGGQEVADSLTRAIGSEVKLLGQIPLDTRLRIGADE
                     GMPVVLGSPDSPAAVALRGIARGLATRSRG"
     assembly_gap    22311..22410
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22414..22908)
                     /locus_tag="PL014_02885"
     CDS             complement(22414..22908)
                     /locus_tag="PL014_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOT56071.1"
                     /translation="MFGVNGWEVVLVALIAVFVLGPDRLPEYAAKLARGIRKARVMAE
                     GAKVQLKDQLGPEYEDINWRQYDPRQYDPRRIVKEALLDPLDDAVAPVREQIGSVRAA
                     GKGLTGGSAAAGAAAAGGAVAASATASAGGGAPVVEEPYISTRATPLEWDPARPTPYD
                     VDAT"
     gene            complement(22957..24345)
                     /locus_tag="PL014_02890"
     CDS             complement(22957..24345)
                     /locus_tag="PL014_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT56072.1"
                     /translation="MTSEREPGRDDWRPWDQASTSDEVTQEVPQQQPEPAWAALPTPS
                     AQGAAPAAAQQAFATQQGPTTHPAPTTQLSPWGAPVAPAPAAPPEAPAPARRGPGWGA
                     LIGVSLAVALVSGLLGGLLGAALTDGESFGSTGSAAGSAPSVGAGATTRPDGSVANIA
                     ARTTPSVVTLRVEGGGGTGTGSGWVYDERGHIVTNNHVVASAADDGEITVVLANGQQV
                     PATIVGRDASYDLAVVKVDRTDLDPLELGRSADVVVGDQVIAVGAPLGLDSTVTSGIV
                     SALNRPVTPGEADDQAFINAIQTDAAINPGNSGGPLLDMQGRVIGVNSAIARAPGSEI
                     SGQSGNIGVGFAIPSDQVATTVEQLITTGKAVHPIIGVFLDSTYDGEGVRIADEGPSG
                     QPAVAPDGPADEAGLRAGDVVIAFEGRPVSDDGELVVLIRSRTVGDSVTLTIMRGGAQ
                     RDVTMVLQGSAD"
     gene            complement(24342..24893)
                     /locus_tag="PL014_02895"
     CDS             complement(24342..24893)
                     /locus_tag="PL014_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56073.1"
                     /translation="MTHSARVQCLDDELTEYAAGRLPLNRQWAWDRHLVTCQVCGHAV
                     DQERRLRSALAGAPTMPGDLRTTLLALGSTMAADLPRTSTPPSPAPDPLRLLAPGAPP
                     CHRSPLRATVIAAAAAGISAAAAWSLTMVPAPGTGRPALATVTPLVAATATPTRAATA
                     AVSNGFSQGVVLTRTGQQAESTP"
     gene            complement(24890..>25252)
                     /locus_tag="PL014_02900"
     CDS             complement(24890..>25252)
                     /locus_tag="PL014_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT56074.1"
                     /translation="RKQRIRFDALSDESAARLEGRGPGPEQAYHDAHFDDDVQRALDA
                     LSPDFRAAVVLCDIEGLSYEEVAATLGIKLGTVRSRIHRGRAQLRESLAHRAPQPTTS
                     APTRSTGRIIRRPAAGVV"
BASE COUNT         3547 a         9099 c         8897 g         3609 t
ORIGIN      
        1 cgccagctcc tcgacaaggg cgtcggagac gacccggcca cggttcaccg accgcgggtg
       61 cgcgatcacg ggcacgccac cggccgcacg cacgatctcg acggcccgca ccgggtcggg
      121 cgcgtagtgc tcgacgtagt aggggctgcg cccacccagc caccgcgcaa aggcctcgtc
      181 acggtgcccg atgaccccgg acgccacgag cgcgtccgcg atgtgcgggc gaccgggagt
      241 ggcgcccggc tcgacctgcg cccggacctc ctcgatcgtc acggggatgc cgtcggccgc
      301 catcttcgcg accatcgcat cgagcctggt gtcgcggctg tggagcgccc gttccagctc
      361 ggccacgagg tcggggtagg tcgggtcggg gaggtaggcc agcaggtgga tgctctggtg
      421 gtgccggctg cacgagacct cgatgcccgg aaccagacgg atgccgtggc gacgcgccgc
      481 acccgcggca tcctcccagc ccagcgtcgt gtcgtggtcg gtgagcgcga cgacgtccag
      541 tccggcagcg accgcctgcg ccacgacgac cgcgggcggc tcggtgccgt cgctcgcggt
      601 cgagtgggtg tgcaggtcga tgaacaccgg cccagcgtag tggcgccccg ggatcacccg
      661 gtgaggcaac cttttcgcag atcgctgcgt agtgcagggt gacaacgtct tcgagggggt
      721 gtgatgggca gcgcgaccaa gagcgaacgt gacgcggcgt tcacggcatt cgtccaccag
      781 gcgtcgccgt cgctgctgcg cacggcatgg ctgctcaccg gcgaccacca caccgcccac
      841 gacctcgtgc aggcggccct cgtgcgcacc tacgtcgcat ggccgcgcgt gcgcgccgac
      901 agcgcgctgg cgtacacccg tcgggtcctg gtgaacgagc gcaccgaccg ctggcgccgg
      961 cacggggccg aggtggctct cgccgcggtg ccggagacca cgtcacccgg gccggagtcc
     1021 gtcaccgacg agcgggatgc cgtggtgcgc ctcctcgccc gcctccccga ccagcaacgc
     1081 cgggtcgtcg tgctgcgcta ctacgtcgac ctgtccgaac aagccacggc cgacgcactc
     1141 aacatctccg tcgggtcggt gaagtcggct gcctcgcgcg gcctggcaac cctgcgggcc
     1201 gagctggcca gcaccaccag tgaaggagtc gtgcgatgag cacggacacg ttcgaggaca
     1261 ccctgcgcga cctgctgcgc gacaccgcgg atgccgccgg gccgggctgc ctggagccgg
     1321 acgccgaccg ggtgatcggg ctgggccggc gcgtgctgcg gcgtcggcgt tgggcggccg
     1381 gcctcgtcgc cgcgagcgtc gccggtgtcg cgggcgtcat gggggtcacg gcactggggc
     1441 cgggggccga tgaatcgtcc ctgcccgcgg ggaccacgtc tgccacgacg tcgggcgtcg
     1501 gagaagtcac cgccgacctg acccgtagcg gctcggtcga gaatgcctgg gggcccgccg
     1561 acgagcagcc gagcgtggag gtcgcggtcg acatgggacg caggaccctc tcgagctcga
     1621 ccctggggca ggacggtcgc acgtcggacg tgcggagtgc gacgctgccc accaaccctc
     1681 gcgcctcgat gtgggtcccg accaccacac cggggctgga gcgcggcgtg ctgccggcgg
     1741 ctgcgaagga tgtcatcttc ctctgggcgg gtgaccaggg cgcgggtacc cacgacctgc
     1801 aaccgctgcc cggtaccccg taccaggcct tcgccgtctg ggtcggcggg gagaacggtg
     1861 gcccgccgcc gctctccggg gtgttgtgga ccgacggcgc agcggtcttt cgcaccgacg
     1921 ggagcgaagt ccacagcgcg cgtgacggag cagcgctggc gttcgtggat cgcgatcagg
     1981 gtgtgttggg catcttcgac gacggctcct catccgtcgc cgcgcttcct gagcgcggaa
     2041 tcccgacgat catgaccggc agccagatgg acggcacgga cacgcttgag acgaccgtcc
     2101 tcctggtgct tccccccggt gtcgacgagg tcggtgtcac ggcagccgag ggtgcgacgc
     2161 tgaggtcgag cgaaacccat cgcgggagtt ccacgcagga cacgctggtc gtggcccacc
     2221 tgtcggtgac cagccctgcc gcggggaccg gggtggacag cgtgacctgg accaacgccg
     2281 acggcagccc gggcgggggg ccggtcagct agttgtgaag tccggggagg gtggcgcgtc
     2341 aggcggggga cgccggtccc ggccagttct tgtccgggac cggtgtgggg cggccgggtt
     2401 gctcaccgcc gcgcgcctgc aggtagttct ccttgggcac catgaccttg cgccggaaga
     2461 tgcacacgat cgtgccgtcc tgcttgtagc cgatggtctc cacgtggacc acaccgcggt
     2521 cgtccttgct cgtgctctcc cacttgtcga gcacggtggt ctcgccgtag agggtgtcgc
     2581 cgtggaaggt cggcgccacg tggcgcaggc tctcgatctc gaggttggcg atcgccttgc
     2641 cgctgatgtc gggcaccgac atgccgagca ggatcgagta cacgtagttg ccgaccacga
     2701 cgttctgacc gaactgcgtg gtctcctgcg cgtagttggt gtccaggtgc agggggtggt
     2761 ggttcatcgt gagcaggcag aacaggtggt cgtcgtactc ggtgaccgtc ttgccgggcc
     2821 agtgcttgta cgtcgccccg acctcgaact cctcgtaact gcgcccgaac tgcaccggct
     2881 gtctccttcg ctcgacgtga ggcgcccatc ctcgcgtgag cacacggcat ccgacagcgt
     2941 tcgggggcgt gccgttgctc acgctgcccg ggccgtcctc ctcgccaccg cggatggcgc
     3001 ggttgtccgc tcagggcgtg aacacatgcc cgtcacgagt ggacaacccc gccagcgccc
     3061 tgcggatctg cgagatcacc cgggtcgggc tggccaggtc tgaccaggtc acgcgcacca
     3121 cgacgtaccc cagcgcgcgg agtgcgtctt cgcgacgctt ctcggccacc agcgccgaac
     3181 gcccctgtgc cccctcgtac ttcacggctc cgtcgaactc gacgatgacg tggtcgccga
     3241 cgaggaagtc cacccggccg acgagagtgc cggacgcatc gcagatgggc acctggctac
     3301 gtaccgccag tccgtggtcg gtgagcatca gtcgcgtcct cgtctctccg acggactcgg
     3361 actgcgggtc ggcgcggctc aggagctggt caccacgggc ccgcagccga cgggaggagc
     3421 atcggtcgtc gagggcgagc gccatctcgc cgagggtgca ctggccggca tgcagcgccg
     3481 cgtcgagggg caccatggca gcaccgacgc ccgacctcac gagcacttgc gcgatccccg
     3541 tcgcgatgtc gacacaccgg taaccgtcgg cgaccacgtg aggctggtcc gcaggtcggg
     3601 cgtgggtgcg cagccccgag gccaggcgca ccctggatac agccgacagg acgtcgacgg
     3661 tgacgaggtc gacgccgtgg agcggcaggc cgtgcagcgc gagggccgac tggtggctgg
     3721 cgatgtcttc gggccgcgcc gcgagcacgg ctcgggtccg cagcatgagg cgctgctcgg
     3781 gcgtcgccgc ggaccagagc tcccccagaa cgtaggcgtc ccggcggaca cggaccacct
     3841 cctgactgcg ccgcccgcgg gccagagcac cgtcgtccac atcgacgacc gccgccatgg
     3901 cgctcgtgaa caccccgccc cgggagccag ccaccgctgt gagcctgtcg tccatgcccc
     3961 tatcgtcccg attcgcggcg gatgggacgc tcggtcatcc acaggcgacg gcctcgacgg
     4021 ggttgtcgac ccgtgacgcc cctgtgcaca cgtcacgggc ggacaacccc gccacgatgg
     4081 cgtctcggat cgtccgccct gcgcgactag gctgccgcca tggcggactt catcggcgcg
     4141 gtcgaccagg gaacgacgag cacgcggttc atcgtcttcg accacgacgg gatgcccgtc
     4201 gcgagccacc agctcgaaca cgaccagatc cttccccgcg ccgggtgggt cgagcacaac
     4261 ccgctggaga tctgggagcg cacgcagacc gtcatcggca gcgcgctgac cagcgctggc
     4321 ctggccacga gtgacctcgc tgcgatcggg gtgaccaacc agcgcgagac cgccatcgtg
     4381 tgggaccgcc gcacgggtcg tccctggtac aacgccgtcg tctggcagga cacccgcacc
     4441 gactcgatcg ccaaggcgct ggatgccgat gggcgaggcg acgtgatccg gcgcaaggcc
     4501 ggtctgcccc cggccacgta cttctccgcg ggcaaggtgc agtggctgct cgagaacgtc
     4561 gagggcctgc gcgccgccgc cgagcggggc gacgccctct tcggcacccc cgacacctgg
     4621 gtcgtgtgga acctcacggg cgggccggac ggcggcatcc acgccacgga cgtcaccaac
     4681 gccagccgca cgatgctcat ggacctcacg accctggact gggacgacga gctgctcggc
     4741 ttcttcggca tcccccgggc gatgctgccg agcatccaca gcagcagtga cgccgccgcg
     4801 tacggggtca cgaccggcga ccgcagtggc gcggccggcg tgcggatcac cggtgtcctc
     4861 ggggaccagc aggcggcgac cgtcggtcag gtgtgcttcg agccgggcga ggccaagaac
     4921 acctacggca ccggcaactt cctgctgctc aacaccggcg aggaactggt gcgcagcagc
     4981 aacggcttgc tgagcacggt ggcctaccgc ttcggcgacg ccaagccggt gtacgccctc
     5041 gagggatcga tcgccgtgac cggcagcgcc gtgcagtggc tgcgcgacca gctcggcatc
     5101 atctccggtg cctccgaggt cgagtcgctc gcggcatccg tgccggacac cggcggggtc
     5161 tacttcgtcc cggcgttcag tgggctgttc gccccctact ggcgaagcga cgcccgcggc
     5221 gccatcgtcg gcctgtcccg gttcaacacc aacgcccacg tggcgcgagc caccctcgag
     5281 gcgatctgct accagtcgcg cgacgtcgtc gacgccatgg ccgccgactc cggggtcaca
     5341 ctcaccaccc tcaaggtcga cgggggcatc accgccaaca acctgtgcat gcagttgcag
     5401 gccaacatcc tcggggtgcc ggtgtccaag ccggtcgtgc cggagaccac ggccctgggt
     5461 gccgcctacg ctgcggggct ggccaccgac ttctggtcgg gcaccgacga actgcgagcc
     5521 aactgggccc aggcgcgacg atgggaaccg cagtggagcg acgcccagcg ccgcgacggg
     5581 tatgccgggt ggcagaaggc ggtcgagcgc accttggggt gggtcgacgt cgagtgactc
     5641 aggcgaggcc gtcggccatc gcgctgtggc agatgtcggc cacctcgttg acggtgcgcc
     5701 gaccatggct gaaccctgcg gtgcttcgcg acaggatgct cagcacgtag tcccggtccg
     5761 ccccgctcac ccaccctgtc gagttcaggc gatagccgtc gggcaggggg ccccagccgt
     5821 tcttggccgc cacgtggacc ttgcgtgact ccggggcgaa gaccccccag cgttgctggg
     5881 gcaccacgtc ggccatgagc atgcgggcga tcgtgcggcg ccccccgtcg agcacccgat
     5941 cagaggacag caccgcatcc accatgagcg agaggtcgcc ggggacggtt cgccagaagc
     6001 cccaccaccc cgagtcgatc tgggtgttcg tcatcccgac gagcccggcg acccgctcga
     6061 cctcatcgcg ccccacctgg gcgagcaggg tctcggtggc cccgttgtcg ctgcgggtga
     6121 tcatccgcgt ggcgaggtcg cgctcgcgct cggtgagctc cacctcggcc tcctgggcct
     6181 gccggagcac cgtggtgagc accaacacct tgatggtgct gtagcagtac ccctcgctcg
     6241 cctcgtgacg aaagctctgc ccccggcgca ggtccacgat ctccagcccc agccgcccgc
     6301 tgcgtcccga caggtactcc tcgagccggt tggcgagctt gggcgcctcc accggtgagg
     6361 cttggccccc tggaacacct gaggcgctcg cagtcgcact gggcgagggg gatgccgtgg
     6421 caccggtggg ttcgccggcc gacccgtcga cggtggtcgg ccccgccgtc gtcaccatcg
     6481 ctggggatgc gacggcatcg tcggtgaaca gttcgcgcac cccgaggccc agcaccccca
     6541 ccccgaggag ggcgccgccc ccgacgacca gccggcgccg ggcgatgacc tcgggtgacg
     6601 gccgccgcga tcgccggatg tcggcccgtc gcgggtcgcg agggcgctgc ggagaagtca
     6661 tcgggaccag cgtacgggcg ggccgtggtg atcgccgaga ccgaggacct caccgtcgag
     6721 ctccacccgg ggaagcccgg gcgacccgct ccccgaggcc gctgcccagt cgtgctcgaa
     6781 cgcgacgtgg gcgagcggca tccgccctcg cgccacccga accgcgtcgt ccacggcggc
     6841 ggtctcctcg gcaggccagt acatccgggt gacggagtgc cacaccacgg tcaggacgcc
     6901 gtcaggcacc ggctcggcga gccgctcggc cacccactgc gaggccgggg cccggtcgac
     6961 gacgaccggg tgctccctcg cgaccgtgag ggccgccgtg agtcgtgcgt ggcgctcacg
     7021 ctgccacggc cacacgaagg aggtcaggta ctcgccaccc tcggcagacg aggtgtcgac
     7081 cggggccagg tcgcatcccc gccgctcgac gacctcgaag gactccggcc ggaacccctc
     7141 tgcaccacat ccgggcacga cgagcgtcgc ctcgaccggg cccgccgacc acccctcacc
     7201 gacgaagcgg tagcggtcga cgagcagccc gagacctccg gaggctccgg gttcgaggag
     7261 tcggacccgg ttcagcccgc ttcggtgcac cgcgtcggac acgccgatga gcagggcgag
     7321 ggtgcgggcc ggttcgttgg tctgcggtgc caccgcgagg ctggagcgca gctcgtcacg
     7381 gcgcgccagc agcaccggcc gcacggcatc ccacgcgtcg cgagggtcga ggtcaccgcc
     7441 cagcgcgggg tagaacccct cgagctgcgg ggcctcgccc cgcaggacga tcctgaagag
     7501 accggcgagc atccgcaggt gcggcatcag ctgcgccggt gcgtcctgcc agccgtcgaa
     7561 gatctcgcgg gtgactccgc cggcttccca gtcgtcggcc atccgtgcga ggagcgccgc
     7621 gtagaggtgg tcctcagggc cgaagtgtgc ccggaaacgg tcggcgagcg tctcacccgc
     7681 gacatccacg acgccctgac ccatggcagc aggctacgac gagaacgcag cgcgtccggc
     7741 ccgtgggagc atggtcgggt gagtgaggaa cagcagcagg cagacaaccg cgcccgggcg
     7801 acgaccgacg agttccgggc cttcgtccgc gacgggtggg caccacgtcg ccccaccgtg
     7861 accgggccga gccccgcggc tgccccggcg gcggctcacc gggcactgat cagcgcggcg
     7921 tacccccgcc agcggctgat catcccggca ggtggcctga aggtgcgcag caacgactgc
     7981 gactacgtct tccgcccgca cacggccttc gcctggctca ccgggctcgg cgcggaccgt
     8041 gagcccgatg ccgtcctggt cctcgaaccc gtggccggcg ccgacggcac tgacggcggt
     8101 cacgaggcgg tcctgtactt ccggccgctg gccgaccgcg acagcgacga gttcttcgcg
     8161 gactcgcgct acggcgagtt ctgggtcggc gcccgaccct ccatcgccga catcgagctc
     8221 gagctgggcc tgacggcccg ccacatcgac gagttccccg cggcggcatc caaggatgcc
     8281 ggcgaggtca ccgtgcgcgt cgtgcgcgac gccgacctcg ccctcacggc acagctggat
     8341 gccgctcggg tcgagaacgg agcggagcag gagggcctga aggagagcga cgccgcgctc
     8401 gcccacgaca tctcccacct gcggatgtgc aaggacgact acgagatcga gcagatgcgc
     8461 gccgcggtcg ccgcgacgca ccacggcttc gacgcgatgg tcgccgaact cgcgacggca
     8521 gccacggtcg agcgcggtga gcggtggctc gagggcacct tcgcgctcca cgcacgccac
     8581 cgcggcaacg gcgtcggtta cgacagcatc tgcgcggccg gggaccacgc gaacacgctg
     8641 cactggatca agaacaccgg cgaggtccgc gagggcgacc tcgtgctcat cgacgccggc
     8701 gtcgaggtcg actccctgtt cacggctgac gtcacccgca ccttcccggt ctccgggacg
     8761 ttcaccgacg cccagcgaga gatctacgac gccgtgtacg ccgcccagga ggccggcatc
     8821 gccgcggtca agcccggcaa caagttctcc gacgtccacg cagcagcgat ccgggtcatc
     8881 gccgagcacc tgcacgcctg ggggctgctc cctgacggcg tctcggtcga ggacaccctg
     8941 gacaaggagc acgggcagta ccaccgccgc tggatggtgc acgggacgtc gcaccacctg
     9001 ggcctcgacg tgcacgactg cgccctcgcg acgcgtgagg agtacatgga tgccgagctt
     9061 cgccccggca tggtgctcac cgtcgagccc ggcctgtact tcaagtccga cgacctgctc
     9121 gcccccgagc ggttccgcgg caccggggtg cgcatcgagg acgacatcct cgtcacgcag
     9181 gacggctgcg agaacctctc ggcgggcatg ccgcgcagct ccgccgacgt cgaggcctgg
     9241 atcgcccgcc tgcgcacctc ctgacttttc ctccccatga ggcaagcgcg aggttcgcaa
     9301 gggatgcatt cccgtgcgaa cctcgcgctt cccgggtgaa gcccgcgcgg agcggtcaga
     9361 gtgtcgtgcg gtcagccccg ccggatgacg tcgaacaggg ccgcgtgctc gacgccggcc
     9421 gcctggcccc caccgccgag aaccatctgg agcagcgccc gaggcttggg gaagtcctcc
     9481 gggagggcgt cgttgtaggc cgcatgggcc tccagtgagt gcacagccgc ctcgaagtgc
     9541 tcgcctgaga cgtccacgaa gtgggtcggg gagctgctcg cagccaccgc gagcgccttg
     9601 accccgcccc acggctccag cccctcctcg gcgagggagg tgtgcaacca ccggttgccg
     9661 gcatccgcga cggcatccaa cgtggccagg ccgaccgcgc ggtggtctgc ctggttgacc
     9721 atgccggtgc cgaaccgctc gtcccacgtg gtggtgacga cgaggtcggg gcgctcgcga
     9781 cggatggcgc gggcgatggc gcaccgcagc gcaactccgt actcgatcgc tccgtcggcg
     9841 aacccgccga actccacgac atcgacgccg acgcgccgtg cactctcgcg ctcctcggcc
     9901 tcgcggacca ctgcggcctc gtcagggtgc atcgtgtcga tacccgcctc gccgcgggtg
     9961 agcaggaagt agctcacggt cgcgccctcg gcaacccatc ggtggacggc cgcagccgtg
    10021 ccgtactcga tgtcgtccgg gtgggcgacg acgcacagga cgcgggcgaa ctcgccctcg
    10081 aagggctcga ggtgggggcg tgcgtcagtc atggggccaa actacgcctc gtcggcgcga
    10141 gcccgctcgg cttcctgcgc ccggcgtacc tcctgcatgg cggcgcgcat cgggtccatc
    10201 tcggtgagca gctcgcggcc gcgttgcacg tgcttgtgct cgcagaggac ctcgtacttg
    10261 gccgccacga cctggctgac ggaggtgaag tcgcgctcac cccgggtgag gcggtacccc
    10321 acggctgccc agaccatgcc gaagagtgca ccgaaggcaa cggtgctgac gacgttgagg
    10381 atgttgaacc cgctcgggtc gaagagagcg aagatcgtcc cgacgaacag acccagccac
    10441 gcgccggaca gggcgcccgc gccgaggacc cgaccccggg tgaggcgccc ggtcacgcgc
    10501 tcgagctgct tgaggtcggt gccgacgatg agcacgtcct ggacggggaa ctcgttgtcg
    10561 gagaggtagt cgaccgcctt ctgggcctcc tcgtaggagt cgtacacgcc cagggacatc
    10621 gggtactgga gcgagagagc ggcccgcagg ccggggcgca tgggctggct ggtcatgtct
    10681 gccattatcc cccggacaac ggccctgcct gcccacgggc ccggccgtgc cgacctcctg
    10741 accttcgcgc tcagcgggcc ttgtagtcct cgagcaggcg gcgaccgatg atcatcttct
    10801 ggatctcggc cgtgccctcg ccgatgagca gcatcggtgc ttcgcggtag agccgctcga
    10861 tctcgtactc cttggagtag ccgtacccgc cgtggatgcg gaaggactgc tcgacgacgt
    10921 ctgcgcagtt ctccgaggcg aggtacttcg ccatgcccgc ctcgaggtcg ttgcgctccc
    10981 cggcatcctt gagcttggcg gccttgacca tcatctggtg gctggcctcg accttggtcg
    11041 ccatgtcggc caggcggaag aggatcccct ggtgctcggc gatcttcttg ccgaaggtct
    11101 cgcgctgctg gctgtaggcg atgccgagct cgaacgcccg gcgcgacagg ccacaggccc
    11161 gggcggcgac gttcacgcgg cccacctcga ccccgtccat catctggtag aagcccttgc
    11221 cgggcactcc gccgagcagc tgcgcatcgg tcgtgcggtg cccctcgaag atcagctcgg
    11281 tcgtgtcgac gcccttgtag cccatcttct cgatcttgcc cggcacggtg acgccctgcg
    11341 cggtctcccc gaagccggac tccttctcga tgaggaaggt gctcatgttc ttgtaggggc
    11401 tctcgctctc accgaggtcg gtgcgcacga gcacggcgac gaggttcgag gacccaccgt
    11461 tggtcagcca catcttctgg cccgtgacga tccactcctc cccctctcga acggccttgg
    11521 tgcggatcgc cgcgacgtcg gaccccagcc cgggctcgga catcgagaac gctccgcgga
    11581 cctcgccggt cgccatccgg ggcaggtagc gctgtttctg ctcctcggtg ccgtgctgga
    11641 ggatcaggta ggcgacgatg aagtgcgtgt tgatgatgcc gctgaccgac atccagcccc
    11701 gcgcgatctc ctcgacgacg agcgcgtagg tgagcagcga ctcgcccagg ccgccgtact
    11761 cctccgggat catcaggccg aagatcccca tctccttcat gccctcgacg atgtccgtcg
    11821 ggtactcgtc gcggtgctcg agctcggtgg cgacgggcag gatctgctcc tcgacgaact
    11881 cgcgcaccag cttgatgagc tcgtgctgtt cctcggagag gccgtccgtg gtctggagtc
    11941 gggacatgcg tgatcgccgc ctttggctcg ggatgggtct gcccaccgag tatgccgtcg
    12001 ggttggccga cgctgaccgc tcagccggtg tgagaccaac cacctccggg tcgatgatgg
    12061 agtcatggca gcccgtgctc ccgcccaccc gcacggtggc cacccgccca ccgtcgaagg
    12121 tcgcctgcgg cctgccctgg ccctgcgccg caacgtcgtg gagttcgcgc agcggtcctg
    12181 gcaggagcac ggggacgtgg tgcgcatggt ggtgggcccc cgcggactgg accgcgagat
    12241 gtggtggctg caccaccccg acgcagcagc caaggtgctc tcgggcgcgt cctggcggtc
    12301 cttcgccaaa cgtgaccagg tctacgacga gatcgccacc tggctcgggc gagggctgct
    12361 cgccgctgag ggtgaggact ggacacgaca acgcagggtg ctccagccgg tgttcacgca
    12421 cgccgcggtc aacgactaca ccgacctcat ggtcgaggag atggagtcgg tcatctcagg
    12481 gtggcccgtc gccgccgccc caaccccggt cgacctcggc gagcggatgc agcacctcac
    12541 cctgcgcgtc gtgctgcggg cactgttcgg tgagtccgcg tcggacgcga cccagcgcgt
    12601 gcgccgttcc ttccccgctc tctccgccgc catcatctcc cgcggcctga cgccactccg
    12661 cctgccggcc gccatcccga ccgccggggt tcggcgcgcc cgagcggccc gcgccgacct
    12721 cgtcgacctc gtggacgcca tggtcgaggc ccgccgcagc ggtcgcacca ccgggggcga
    12781 cgacctcctc ggccacctgc tcacggcgcg tgacggcgag cgcctgctct ccgacgggga
    12841 ggtgcgcgcc caggccctgg tgttcctcct cgcggggcac gagaccacgt ccagcgccct
    12901 gacctacaca ctgcacctgc tgggtcgtca tccggacgtc caggaccgcc tgcgcaccga
    12961 ggtgcacgac gtgctcgggg agcggcgccc ctccgccacc gacgcggcat cactccccga
    13021 gacgacggcg gcgctcaagg aggccatgcg cctctacccc tcggcgccgg tcctcggtcg
    13081 gctcgccgtc gaggacgacg agatcatggg ctaccccgtg gcggcgggta cctccgtggt
    13141 cgtggcgccg tggaccatcc accgccaccc cgacttctgg gtcgatcccc tgatctacga
    13201 cccgtcgcgc ttcacgacga acggacctcg gccgcagccg gcacaccgct atgcgtggat
    13261 gccgttcggt gggggtccac gggcgtgcat cggccaacac ttctcgatgg tcgagtcggt
    13321 gctcgccctg gcgctgatcc tgcgcagcca ccgcgtcgcc ccgacgaggt ccaccgacgc
    13381 cgaccacctg ccggtcggcg cccacctgac gctcgtcccc acgacgccgg tgctggccac
    13441 gctgtcccga ctcccctgac cacgcgatcg ccatgacgcg ggaaaaccag tggcgaccgg
    13501 cgccgggggt gcggcacgct gggaggtgag cacacggcat ccgccgtcgc tgagcaccag
    13561 accctgatcc ggaagaggag gccgacatgt cgacgactgt ccgggcccac accgagcccc
    13621 tccttccctc tctcgatcag gagcaccccc gtggcacacc agccgttcca cctgccggcg
    13681 aagcaggagc tcttcgccac cgacacctca cctgacgccc ccaccgaggg cgagcgctgg
    13741 tccacctggg acggcgcgac ccacggcccg aagccgcgcc cgtcctgggt gatcaccgac
    13801 ctcggcgccg tcgaggccga tctcggcgtg ctgaagaccg gcaaggaagc cgacgtgcac
    13861 gtgctgcgcc ggtgggtccc gggcaccgag cgcagcagca ccatggccgc caagcgctac
    13921 cgcagcggcg agcaccgcct cttccaccgc gatgccggct acctggaggg ccggcgggtg
    13981 cgcaagagtc gggagatgcg tgcgatggcc acccgcaccg agttcggcaa gcagctcatc
    14041 tcggggcagt gggccgcggc cgagttcagc gcgctcggca ccctctgggc cctcggcctg
    14101 ccggtgccct accccgtgca gctcgacgag accgagatgc tcatggagtt cgtcggtgac
    14161 gtcagcggcc cgacgcccgt ggccgccccc aggctcgtgg cgaccaggcc tgatcccgac
    14221 ctgctcgccg acctcttcga gcagctgcgc accgccgtgc tcacgctcgc gcaccacggg
    14281 tgggcccacg gcgacctgtc gccctacaac gtgctgctgc acgacgagcg gctcgtcatc
    14341 atcgactggc cccagatcgt cgacgtcatc ggcaacccgc agggcttcga cttcctggcc
    14401 cgcgacgcga cgacgatggc gacgtggttc acccggcgtg gcctggacgt cgacgccggc
    14461 gagttcgtcg gcgacctcgt ggccgaggcg acgtcccgct tctgaggtgg gcggacggca
    14521 tacgatcagc cgcatgtcgt ccgcccccgc tgcccccgca accctcgccg cgtccacggt
    14581 tcccgacccg atggacgccc cggcgatccg ctggggcgtc ctcgccccgg gcggcatcgc
    14641 caacaccttc gcggatgccg tggccacggg cacgcggtcg cgcgtcgtcg cctgcgggtc
    14701 gaggtccctg gatcgggccc gggagttcgc gcagcgccac ggggtcgagc gcgcccacgg
    14761 gtcgtacgcc gacctcgtcg ccgacccgga cgtggatgcc gtgtacgtcg ccagcccgca
    14821 cagcgagcac cacgagcacg ccctgctcgc cctgcgggcc ggcaagccgg tgctcgtcga
    14881 gaaggccttc acccgtagcc tgcgcgaggc cgagcacgtc ttcgacgccg cgcaggcagg
    14941 tggcctgctc acggccgagg cgatgtggag ccgctacctg ccgcactacg acgtgatcgc
    15001 ccgcacggtc gccgccggca cgttgggtga cgtcgtggtc gtcgaggccg accacggcca
    15061 gctgctctac cccgacggcc cggcgcgcct gtcccagccc gagctcgccg gtggggccct
    15121 gctcgacctc ggcgtctacc ccgtctcctt cgccgaccac gtgctcggca gcctcgccga
    15181 cgtgacggcg cgtggcgtcc tcaccgacct cggggtcgac gccacgacga ccatcgacgg
    15241 ccgcggcccc tccggggagc tcgcccggct gtggtgctcg atggccgcgg ccaccgcgtg
    15301 ccccgcacgc gtggtcggca cggccgcacg gctcgagatc tcgggcgtct tctacgcggt
    15361 cgacagccgc gtgcgcctcg tggcaccgga cgacaccgtc ctcgacgagt tcgtcgcacc
    15421 ggtgctcgac cacgggttcc gctaccaggc cgcggagttc gcgcgggccc tggcggcggg
    15481 gcgcacgcag acgtggtcga tgccgtggga cgccacccgt cgcgtcatgg ccaccatgga
    15541 cgagacccgc cgtcaggtcg gggtcgtcta ccccggcgag tgacgcctca ccggggcgtc
    15601 agcgcacgag gtcgccgggg cgtcagcgca cgacgaccgt gctcccgatg ggcatgaggt
    15661 tcttgtccca gatcatgttg atcgccgcgt tgctcaaccg ggcgcacccg tgggaggccg
    15721 gccacggcgg gatgttcggc gagccgtgca cggcgatgcc gcggtagaag aagcgagggc
    15781 gccacagctg acccagcgag ttggtcagcg gcccgtcgac cccgcggtag accttgaagg
    15841 tccccttcgg cgtgacggcg atggcccggt tgcccgaggt ggaggtgtac tcctcacggt
    15901 tgcccgtcga ggtgttgagg atcgtcctca ccgtgccacc gcgcacgacg agcagcagct
    15961 ggcggtccag cacgatctcg accccgttgc cggacagggt cgcccgcggt cggatgccgt
    16021 cggcgagcgc cttggcggtc ttggggccga ccgaaccatc acgtgagagg ccggcagcct
    16081 tctgcagggc gaagaccgcc tgctgggtga gggcaccgaa gtggccgtcc ggcgtaccga
    16141 gccagtagcc gagctcggtg agccgctgct gggtggccag caccttgggc ccccgatcac
    16201 ccagccgcaa ggtggtgcgc ggcttggccg tcggcgacgg ggaagggctg gacttcgcgg
    16261 acggcttcgc cgagggcttg ggggacggct tgggagcctt cgtcgtgctc ggcgatggtt
    16321 tcggggctgc gctggtggtc ggtgaggggg tggcggacga cgcgctctgc gtcaccgtgg
    16381 gcaccggggt ggatgccgtc ggggcgctca ccgacggggc ggcactggac acggacggcg
    16441 acggcgtgtc cggggcgacc gacggcgggg ccgcgctggt cgtgctctgc tgcggtgcgg
    16501 ccgcgtcgga ggaccaggac gcgccgagcc ccgcacatcc cccgaggacg agcgcacccg
    16561 cgagggccag accgggcgtc gccgcgcgaa cgctcaggtg accaggcatc agcgaaactc
    16621 cagacgtgtc gaaccgacca acggtgccaa tcatccctca ggtgagggca tcgaggccgg
    16681 attgaggtcc gacgcggcgg tgcgcgcccc cctcaggcgc ggtcggcgat ggcctgcgcg
    16741 gtcgcgatga cctggcgggc gatctcgacg tgcaggtgct cgaccatgcg cccacggtga
    16801 gtgacgacac cgctgccggc accggcatcc caagccgcca cgagagcgtg cgcgtcctcg
    16861 atttcggccg gtgacggcgc gaagacctcg ttgcacggcc cgacctggcc ggggtggatg
    16921 agcgtcttgc cgtcgaagcc catgtcagcg ccctggcggc actccgcgaa gaacccgtcg
    16981 aggtcggtga cgtcgttgta gacaccgtcg aggatcgcga cccccgagcg tcgggcggcg
    17041 agcagggcga gcgacaacga cgtcagcagt ggctctcggc cgggaacgtg ccgggccccg
    17101 agttccttga cgaggtcgtt ggtgccgatc accagggcag cgagtcgagg tgacgctgcg
    17161 gcgatctcac ccacggcgag caccgccgcg gggctctcga tcatggccca caaccgggtg
    17221 tgctcgggtg cgccgtgccg ctccatggcg gccacgaggt cgagcacctc ctgcgccgag
    17281 tcgaccttgg ggacgacgat cccgtcaggg ccggcggcgc aggcggcggc gaggtcgtcg
    17341 tcgtgccact gggtgccgct cgcgttgacc cggatcgtca cctcgcgctg gccgtagtca
    17401 cccgagcgcg cggccgcgca cgcgttgtcg cgggccgtgg ccttgtggtc aggagccacc
    17461 gcatcctcga ggtcgaggat gaggccgtcg acgggcaggg tcttggcctt ctccagggca
    17521 cgctcgttgc tgctcggcat gtacagcacc gatcgacgag gccggtagtc cgtcgcggcg
    17581 ctcatgcctg cgccccggca tccgtcgtgc ccgcggcacc ggcatagagc gcggcgagct
    17641 cagggtcgtt ggccgagagc cggtcggcga gctcgaggat gaccaggcac tgcttcaggc
    17701 tcgcgtcgtc ctccatcttc ccgtcgagca tgaccgcccc cgtcccgtcg cccatggcct
    17761 ccttgacccg ccgcgcgtgc gcgacgtcct gcgcgctggg gctgaacacg gtcttggcga
    17821 tggcgatctg cgcggggtgc agggtccacg tgccgacgca gccgaggagg aaggcgttgc
    17881 ggaactggtc ctcacaggcg acgacgtcgg cgatgtcccc gaaggggccg tagtacggaa
    17941 agatcccgtg catcgcgcag gcgtcgacca tgcgggcgat cgtgtagtgc cataggtcct
    18001 gctggaaggt ggggcgggag ccggtgatgt ccccgccgac ggggtcggca cgcacgaggt
    18061 agccggggtg tccgccaccg acgcgggtcg tcttcatccg gcggtcggcg gcgaggtcgg
    18121 cggggccgag gctgatgccc tgcatccggg ggctggcacc cgcgatctgc tcgacgttcg
    18181 cgacgccgcg ggccgtctcg aggatcgcgt ggacgaggat cggccgggtc agccccgcct
    18241 tcgcctcgag ctgggccagc agccggtcga cgtagtggat gtcctccggc ccctgcacct
    18301 tgggcaccat gacgacgtcg aggcggtcgc ccacctcggt gacgagggtc gtgaggtcgt
    18361 cgagcaccca cggtgagtcc agggagttga tccgtgtcca cagctgggtg tgctcgccga
    18421 agtccgtggc gcgggcgatc tcgaccaggc cggcgcgggc ggcatccttg ttctccgcct
    18481 tgacggcatc ctcgaggttg cccagaagca cgtcgaccgt cccgacgagc tggggcacct
    18541 tggccgccat ccgcgggttg ctggggtcga agaagtggat catccggctt ggccgcgccg
    18601 gcacctcccg cacgggtacg ggtgcgccca ccgcgagggg cgtgaagaag tccttggcgc
    18661 tgcgcatgcg ggggtccctt gtccacgagg tcggcgagat gcaggtctgg ccgggacgct
    18721 agcagcgccg cccggtgggg cgttgtgaga gagttcacgg tgtgacggat gacgcttcca
    18781 cgcaggtcac gccgggtgcc ccgaccatta acgtcacccg gctgatccac gaggccgcga
    18841 gcaagtcggg cctgctctgg atccgcgtgc ccggcgccgg cacccacccg gtctggcacg
    18901 tgtggcacga cgacggcgac gagcgcggca cggggccggc tgcctacgtc gtgtccgggc
    18961 ccggcgagca gagcctgccg tggttgcccg acgaggtgga gctggtcctg cgcagcaagg
    19021 acagcggtgg gctgctgctc agcgttcgcg cgtcgtcgcg cgagctcact cccgcggacg
    19081 aggactggga tgccgcggtg caggtcctgc gccctgagcg cctcaatgcg gtgggagacg
    19141 tcgcgcagcg atgggcccag gggtgcacga tccacgtgct caccccccac ggcaggctcg
    19201 tccacgggcc cggggcgtac ccgtccgact ccggcagcat gtcggtcccg tccgggccgg
    19261 ggaccaccgc tcggtggcgt ccgtggcact ggcgcggccg ggccggcgcc cgccgcaaca
    19321 ccccgcccgc ctgaccgcct cggcggctcc acaccccgat agcctgtgcc agtgagcacg
    19381 ccgacccgcg ccttcgtcgg ccgcctgacc gagctgagcg tcttcgaccc cctcggtgac
    19441 caggtcggcc gggtgcgcga cgtcgtcgtg acgttcaacg tcgcgcggac ccgtccgcgc
    19501 gtcatcgggc tcgtcgtcga ggtggccggc cggcggcggg tgttcgtgcc gatgacccgg
    19561 gtcacctccg tcgagggcgg tcaggtgatc accaccgggc tggtcaacat gcgccggttc
    19621 gagcagcgcg ggaacgagac gctcgtggtc gccgagctgt tcgagcgccc cgtcgaggtg
    19681 cgcaccgagg agggtgggct gctcgcggcg acggtcgagg acgtcggcat cgagcagcac
    19741 ggctcccggg gctggagcgt ggcccgcgcc ttcgtgcgca ccggcgcccc ccgtggcggc
    19801 ctgggtcgac tggcccgccg gcggggtgag accaggctcg tggcgatcga ggacgtcgtc
    19861 gggctgcgca cgtcgagcag tgcgcagagc gccgctcggc tggtcgaggc ctacgaggac
    19921 ctcaagcccg cagacctcgc cgaggtcatc cacgacctca ccccgcagcg tcgggccgag
    19981 gtcgccgctg agctcagcga cgagcggctc gccgacgtca tcgaggagct ccccgaggac
    20041 gaccaggtcg agatcatcgc cggcctgcgg gtggaccgcg cggccgacgt cctcgaggcg
    20101 atggagcccg acgacgccgc cgacctcctg gccgagctgc ccaaggaccg ccaggaggag
    20161 ctcctcgagc ggatggacgc cgacgaggct gccgacctgc gacgtctgct gacctacgac
    20221 gagaacaccg ccggtggcct catgacgacc gagccggtga tcctcggccc ggaggcgtcc
    20281 atcgccgagg ccctggccgt cgtgcgacgt caggagctgt ccacggccct ggcctgcacg
    20341 gtcttcgtct gccgctcgcc gctggagacc cccacgggtc gctacctcgg catggtccac
    20401 atccaacgcc tcctgcgcga gcccccgcac aacccggtgg gcggcatcct cgacaaggac
    20461 gtcgagccgc tgctccccga cgccaccttg ggccaggtga cccgcatgct cgcgacctac
    20521 gacctggtcg gggtgcccgt cgtcgacgcc gagggtcacc tgctcggcgc tgtcaccgtc
    20581 gacgacgtgc tcgaccacat cctccccgac gactggcgcg aggaccggca cgacagcgtg
    20641 acgcccgagg gggccacccg tggctgagcg cgagcggcga ccccgcatcg accagccccg
    20701 agaggggggc cgccgcttca ccgtgccctc ggtgttcacc gaggaggcct tcggggtcgt
    20761 ctcggagaag ttcgcccgct acatgggcac gccctacttc ctcatcggca tgaccctgtt
    20821 cgtgctgatc tgggtgggct ggaacgtcct cgccccgcca tcccagcggt tcgacaagta
    20881 cccgttcatc ttcctcaccc tcatgctcag cctgcaggcc tcgtacgccg cgccgttgat
    20941 cctgctcgcc cagaaccgtc aggccgaccg cgaccgcttc gccctcgaac aggaccgcag
    21001 ccgcgacgag cgcaacctgg ccgacaccga gttcctcacc cgagaggtcg cggccctgcg
    21061 gatcgcgatg cgtgaccagg ccacccgcga cttcgtgcgc ggcgagcttc gcgacctcct
    21121 cgacgagatg gaggaacgcg gcctggtggt ggtgcgcgca cctgagtccg acgaggcccc
    21181 cgtccgctcc cccggttcgc cgacctagac tggggggcat gtcccccgtc acccccgacg
    21241 ccctgcacgc tgccctcgag acggtcatcg accccgagat ccgcaagccg gtcaccgagc
    21301 tcggcatggt cgagagcgtg tcggccgacg agtccggcca cgtcgccgtc accatcctgc
    21361 tcaccgtggc ggcctgcccg ctcaaggaca ccctcacccg cgacaccacc gcggccctgg
    21421 agaaggtcga gggggtcacc ggcgtcaccg tgacgctcgg ggtgatgagc gacgaacagc
    21481 ggaccgagct gaagacgaag ctgcgcggtg gtgtcgccga gcgcgaggtc cccttcgcca
    21541 aggccgggtc gctgacgcgg gtgtacgccg tcgcgtccgg caagggcggt gtcggcaagt
    21601 cctcggtcac ggccaacctc gcggctgccc tggccgagca gggcctgcgg gtcggggtcg
    21661 tcgacgccga catctacggc ttctcgatcc cccgcatgct cggggtcgaa gggcgcccga
    21721 cccaggtcga cgacatgatc ctgccgccgc tcgcgcacga cgtgaaggtg atctcgatcg
    21781 gcatgttcgt gcccggcaac cagcccgtcg tctggcgtgg gccgatgctg caccgggccc
    21841 tccagcagtt cctcgccgac gtcttctggg gtgacctcga cgtcctgctc ctcgacctgc
    21901 ccccgggcac gggtgacatc gcgatctcca ctgcccagct cgtccccggc gccgagatcc
    21961 tcgtcgtcac caccccgcag caggccgctg ccgaggtggc cgagcgggcc ggcgccatcg
    22021 cgctccagac ccaccagcgc gtcgccggtg tcatcgagaa catgagctgg ctcgagcttc
    22081 ccgacggcac ccgtcaggag atcttcgggt ccggtggcgg ccaggaggtc gctgactcac
    22141 tgacccgcgc gatcggttcg gaggtcaagc tcttgggcca gatcccgctc gacacccgcc
    22201 tgcgcatcgg cgccgacgag gggatgccgg tcgtcctggg ttccccggac agcccggcag
    22261 ccgttgccct gcggggcatc gcgcgtggtc tggcgacgcg ctcgcgcggg nnnnnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggtcaggtg gcgtcgacgt cgtagggcgt
    22441 cggccgtgcc gggtcccact ccagcggcgt ggcccgcgtg gagatgtagg gttcctcgac
    22501 cacgggcgcc ccacctcccg cggatgccgt ggcggaggct gcgaccgcgc ccccggccgc
    22561 tgccgcaccg gcagccgctg aaccacccgt gagccccttg cctgccgccc gcacggaacc
    22621 gatctgctcg cgcaccggcg ccaccgcgtc gtcgagcggg tcgagcaggg cttccttgac
    22681 gatccggcgg gggtcgtact gacgagggtc gtactggcgc cagttgatgt cctcgtactc
    22741 ggggccgagc tggtccttga gctggacctt ggcgccctcc gccatgaccc gcgccttgcg
    22801 gatgccgcgc gcgagcttgg cggcgtactc gggcaaccga tcaggtccga ggacgaagac
    22861 ggcgatgagc gcgacgagca cgacctccca gccattgacc ccgaacaccg agcaccaccc
    22921 gtctctttcc tggaccgctt ccgcgccgtg ggcgcgtcag tcagccgaac cctgaaggac
    22981 catggtgaca tccctctggg ccccgccgcg catgatggtc agcgtcaccg agtcacccac
    23041 ggtccgagag cggatcagga cgaccagttc accgtcgtcg gagacgggcc tgccctcgaa
    23101 ggcgatgacg acgtcaccgg cccgcagccc ggcctcgtcc gcggggccgt ccggtgcaac
    23161 cgcgggctgc ccggaggggc cctcgtcggc gatgcgcacg ccctcgccgt cgtaggtcga
    23221 gtcgaggaac acgccgatga tggggtgcac ggccttgccg gtcgtgatca gctgctcgac
    23281 cgtggtggcc acctgatcac tcgggatggc gaagccgacg ccgatgttgc cgctctggcc
    23341 gctgatctcc gaccccggcg cccgggcgat ggcggagttg accccgatga cccggccctg
    23401 catgtcgagc aggggcccac cggagttgcc cgggttgatg gcggcgtcgg tctggatggc
    23461 gttgatgaac gcctggtcat cggcctcgcc gggtgtgacc ggccggttga gggcgctgac
    23521 gatgcccgag gtcacggtgg agtccaggcc gagcggggca ccgacggcga tgacctggtc
    23581 accgacgacc acgtccgccg agcgacccag ctcgagcggg tcgaggtcgg tgcggtcgac
    23641 cttgacgacg gcgaggtcgt acgacgcgtc gcgaccgacg atcgtggccg gcacctgctg
    23701 accgttggcc agcaccaccg tgatctcccc atcgtcggct gcgctcgcga cgacgtggtt
    23761 gttcgtgacg atgtgaccac gctcgtcgta cacccagccg gagccggtgc cggtgccgcc
    23821 gccaccctcg acccgcaggg tgacgacact cggggtcgtg cgagcggcga tgttggcgac
    23881 ggaaccgtcc gggcgggtcg tggcccccgc acccacgctc ggggccgaac cggccgctga
    23941 tcccgtggag ccgaaggact ccccgtcggt cagggccgcg ccgagcaggc caccgaggag
    24001 accggagacg agcgcgacgg cgagcgacac cccgatcagg gccccccagc ccggcccgcg
    24061 gcgcgcaggg gcgggcgcct cgggcggagc agccggagcc ggagcaaccg gggcacccca
    24121 cggcgagagc tgggtggtcg gcgccgggtg cgtcgtgggg ccctgctggg tcgcgaaggc
    24181 ctgctgggcg gcggcagggg ccgccccctg cgcggacgga gtcggcagcg cagcccacgc
    24241 aggctccggc tgctgttggg gcacctcttg ggtcacctcg tcagaggtgc tcgcctggtc
    24301 ccaggggcgc cagtcgtcgc ggcccggctc gcgctcactc gtcatggggt cgattctgcc
    24361 tgctggccgg tcctggtgag gacgaccccc tggctgaacc cgttgctcac ggccgccgtg
    24421 gcggcgcgcg ttggcgtggc cgtcgctgcc acgagggggg tcacggtggc cagggcaggc
    24481 cgaccggtgc cgggtgcggg aaccatggtc aggctccagg cggcggctgc ggagatcccg
    24541 gcggccgcag cggcgatcac cgtggcgcgc agcgggctgc ggtggcaggg cggagcaccg
    24601 ggagcgagca gccgcagggg gtcgggcgca gggctcggcg gggtgctcgt gcggggcagg
    24661 tcagcagcca tcgtgctgcc gagcgcgagc agggtggtgc gcaggtcccc cggcatggtc
    24721 ggggctccgg ccagggcgct gcgcagtcga cgctcctggt cgacggcgtg gccgcagacc
    24781 tgacaggtca cgaggtgccg gtcccacgcc cactggcggt tcaggggaag gcgcccggcg
    24841 gcgtactcgg tgagctcgtc gtcgaggcac tgcacccggg cggagtgggt catacgaccc
    24901 ccgcggcggg tcgacggatg atccggcccg tgctcctcgt gggggcgctc gtggtcggct
    24961 gcggtgcgcg gtgggccaac gactcgcgca gctgggcgcg accgcggtgg atgcgcgagc
    25021 gcacggtccc gagcttgatg cccagggtcg cggcgacctc ctcgtaggac agcccctcga
    25081 tgtcgcacag cacgaccgcg gcccggaagt ccggggagag tgcgtcgagc gcacgctgca
    25141 cgtcgtcgtc gaagtgggcg tcgtggtacg cctgctcggg gccgggcccg cgcccctcga
    25201 ggcgggcggc cgactcgtcg gagagggcgt cgaagcggat ccgctgcttg cg
//
LOCUS       DAOSOC010000019        25163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_1983_length_25163_cov_9.542337, whole genome shotgun sequence.
ACCESSION   DAOSOC010000019 DAOSOC010000000
VERSION     DAOSOC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 25163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25163
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1983_length_25163_cov_9.542337"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PL014_02905"
     CDS             <1..255
                     /locus_tag="PL014_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOT56075.1"
                     /translation="EVMFYVDGDYEARKGSGIAKGSISLHPGGHAHGPQPGAAEASIG
                     KESFDELAVMVDTFRPLEIGEGGRACDDGVYAGSWNRPRA"
     gene            332..1099
                     /locus_tag="PL014_02910"
     CDS             332..1099
                     /locus_tag="PL014_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56076.1"
                     /translation="MSDVLTLHGLGCSVEVRCSGDAAAPLLEALRAAWSRCLVADGRG
                     EPRAANPVEARLDDEGDLAHRLMATTQTVTRALIAAQAGHLLMVHAGAVGHPLTGVSL
                     VYVAPGGTGKTTLSRRLGQSYGYLTDETVGMDADGRILPYPKPLSVRRQEQPGVKDEL
                     SPDALGLLAAPPAPVASRVVLLARDPAATAADVEEVPFMDAVFALTEQSSSLAALPRP
                     LHRMADFIDAAGPVLRLRYAEAADLDGTLTALLGGAA"
     gene            1096..1359
                     /locus_tag="PL014_02915"
     CDS             1096..1359
                     /locus_tag="PL014_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56077.1"
                     /translation="MKVQRCPVDDLLTRDGETAVLAHGKALRLSAVAVAIYDLTEHEI
                     EVESLAEALESQVGAPADRSTLEATEAAVAMMVRHGILTRSSR"
     gene            1391..1681
                     /gene="clpS"
                     /locus_tag="PL014_02920"
     CDS             1391..1681
                     /gene="clpS"
                     /locus_tag="PL014_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOT56078.1"
                     /translation="MSLAPVTQEVTATGGDLAPDLPWVTIVWNDPVNLMSYVTYVLQS
                     HFGYSRAKAEKLMMDVHTQGRAVVANGTREAMEADTEALQGYGLWATFQKDS"
     gene            1681..2313
                     /locus_tag="PL014_02925"
     CDS             1681..2313
                     /locus_tag="PL014_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HOT56079.1"
                     /translation="MARGFRRKGRGRAASYVARLDPLERATVAGLMEQVHDLLEPPPT
                     TTVGPGSEGPVDEFAAIVAGLGMGVTVAAEDQADLVASGAGRDPALDRLLPTANRQDD
                     EAAAEFRRLTETGLRQRKAAGLALAASLLRVEDKVVLDEASARAFLTALTDVRLVLGE
                     RMGLRTDEDLEALEAAAGELDPDDPLGYALAVYDFLTWLQETLASAMLTA"
     gene            2440..3267
                     /gene="murI"
                     /locus_tag="PL014_02930"
     CDS             2440..3267
                     /gene="murI"
                     /locus_tag="PL014_02930"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOT56080.1"
                     /translation="MADQPIGVFDSGYGGLTVMRAVLDQLPHESVAYFGDTARAPYGP
                     RPIAQTRELALECLDRLVDHGVKALVIACNTASAAVLHDARERYDVPVIEVIRPAVRR
                     AASATRNGRIGVISTVGTHQSGAYLDAFAAAPHLDVVSTPCPRFVEFVERGITGGSEL
                     VAVAHTYLDPLREQGVDTLVLGCTHYPLLAAVITYVMGDGITLVSSAEETAKDVYRVL
                     ADADLLRPDGLPPPDHGFTTTGDPDEFRRLAARFLGVGSRLGPVFTAPLPLDVEGPE"
     gene            3264..4031
                     /locus_tag="PL014_02935"
     CDS             3264..4031
                     /locus_tag="PL014_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT56081.1"
                     /translation="MRVTVIGCSGSFAGPSSPASSYLVQAEFGGRTWSILLDLGSGAL
                     GALQRQMSPDDLDAVLITHLHPDHCVDLCGLYVSRRYRPFGPLPARLPVFGPTGTARR
                     LQLMYHGLEPGGMEAEFDIHTVVDAAPILIGPFTVTPYRVNHPVEAYGYRVEAGGALL
                     AYTGDTDDCPNLDPLLRGVDLALMDSAFIDGRDDVVDIHLSGSRAARAALRAGGVQSL
                     VLTHIPSWNDPEVCRAQAAMHWPSAVGLAAADAVYEI"
     gene            4138..4896
                     /gene="rph"
                     /locus_tag="PL014_02940"
     CDS             4138..4896
                     /gene="rph"
                     /locus_tag="PL014_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOT56082.1"
                     /translation="MPSDSIRHDGRAPDQLREVRITRNWLDHAEGSVLVEFGRTRVLC
                     AASFTAGVPRWLKGRGTGWVTAEYEMLPRSTNTRSDRESRRGKVGGRTHEISRLVGRS
                     LRAVIDTKGLGENTIVLDCDVLQADGGTRTAAITGAWVALADAVSDARSRGLIPPSAQ
                     PLTGSIAAVSVGLVDGLPVLDLDYPEDSTAQTDMNVVMTGDGRFVEVQGTAEGEPFDR
                     AVLGDLLDLATVGCAELTLLQQAALAATPRERRA"
     gene            4893..5531
                     /gene="rdgB"
                     /locus_tag="PL014_02945"
     CDS             4893..5531
                     /gene="rdgB"
                     /locus_tag="PL014_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOT56083.1"
                     /translation="MSRRLVLATRNEHKVHELRQILGSLVTDLDLEIIGAGDVEGAPD
                     VAETEVTFLGNARLKAVALAEATGLPALADDSGLAVEVLGGSPGVFSARWSGSAAGPG
                     ATRAEVDRANLHLLLEQLGDVPDEHRAAAFVCAAVLAMPDGRVHGVEGRVEGRLARAP
                     EGHHGFGYDPVFVPTGQQRTLAEHTDEEKNAISHRGRAFRALEPVLRELLGG"
     gene            5560..5644
                     /locus_tag="PL014_02950"
     tRNA            5560..5644
                     /locus_tag="PL014_02950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5594..5596,aa:Leu,seq:tag)
     gene            5735..6205
                     /gene="bcp"
                     /locus_tag="PL014_02955"
     CDS             5735..6205
                     /gene="bcp"
                     /locus_tag="PL014_02955"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOT56084.1"
                     /translation="MGDRLSPGDSAPDFTLPDDTGTPVTLSALRGGKVIVYFYPAAMT
                     PGCTTQACDFSESLESLGAHGYTVLGISPDTPEKLARFREKDALTITLLSDADRAVMT
                     AWGAFGEKKLYGKTVQGVIRSTVVLDEQGIVTHAFYNVKATGHVAKLRRDLGLQ"
     gene            6213..6524
                     /locus_tag="PL014_02960"
     CDS             6213..6524
                     /locus_tag="PL014_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HOT56085.1"
                     /translation="MSNVQPAATGPSTGAAAPAPVTIADLEAEITARRDRLAQTIDEL
                     AERVRPSSIIQRQTDVAKQRFADVATTPSGELRVERLAAVVAVAAVVGSWLIVRRLRR
                     G"
     gene            complement(6673..6748)
                     /locus_tag="PL014_02965"
     tRNA            complement(6673..6748)
                     /locus_tag="PL014_02965"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6713..6715),aa:Lys,seq:ctt)
     gene            complement(6814..7464)
                     /locus_tag="PL014_02970"
     CDS             complement(6814..7464)
                     /locus_tag="PL014_02970"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HOT56086.1"
                     /translation="MQSRRFATVVTLVIVVAFFLVAIAPAFASPAPGTLPFHARLVSS
                     EPADRTSVETADRVVLTFNESINPDFVAVRVSGPEGAEVDGAPSVEGATVTQPLAAPL
                     AAGEHTVTYRVVSNDGHPISGTVTFTSMAVAATASPTASTPTSTPEASPTPTVPTASA
                     TPMPSDSPTTTPEPVADEAGTSPWVGVGIAVGVAALAAVLGLVWHRRRSRPDDLRD"
     gene            complement(7574..7649)
                     /locus_tag="PL014_02975"
     tRNA            complement(7574..7649)
                     /locus_tag="PL014_02975"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7614..7616),aa:His,seq:gtg)
     gene            complement(7711..8397)
                     /gene="orn"
                     /locus_tag="PL014_02980"
     CDS             complement(7711..8397)
                     /gene="orn"
                     /locus_tag="PL014_02980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOT56087.1"
                     /translation="MATPGTDRALSDRIVWIDCEMTGLSLTSDALIEVAALVTDYELT
                     VLGDGIDVVIRPPDEALDQMDDFVRDMHTTSGLLDVLAAGTTLERAQEMVLDYVREWA
                     PEARKSPLGGNTVATDRAFLARDMPELESHLHYRIIDVSSIKELSRRWYPRAYFAAPS
                     KSGGHRALADIRESIAELRYYREAVFVAKPGPSTDQARALAARHVVDHEAASTAAAPT
                     DGTVVESTLS"
     gene            8474..9643
                     /locus_tag="PL014_02985"
     CDS             8474..9643
                     /locus_tag="PL014_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOT56088.1"
                     /translation="MSGDLSTRPPDLRVRRNPTARGALRGWVLTGIAGLGFLIVALVV
                     AAYFDAAFGVQASLLALAAAVVPLGIVIPTFLWLDRFESEPNRLLVGAFLWGALVAAV
                     VSALLNTTAMSLIEAMSTADPDAALTTTAVLVAPFVEEAAKGVLVLVVWWFLHHEFDG
                     ITDGMVYAGICAAGFAFTENIQYLAQAWTEGGSELLTGTFIARCLFSPFAHPMFTVLT
                     GIGIGIAATSRSWMPRLLAPVAGFLLAVLSHGLWNLAAVTGGEGMVVIYLFVQVPIFC
                     AFLAFVAWVRRTEGHLIGQFLRPYADVGWLSPAEVAMLSSMPQRREARAWARANTGRE
                     GLKAMRAFQDAASELALLRRRMSHRAADAHAIEQEHELLLALRDRRAEFIGLPAS"
     gene            9665..10894
                     /locus_tag="PL014_02990"
     CDS             9665..10894
                     /locus_tag="PL014_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOT56089.1"
                     /translation="MTDTLRIAARLAPELVALRRHLHGIPELGLDLPDTQAAVLEALA
                     GLDLEVTLGESLSSVVAVLRGRAPVTGERPVVLLRGDMDALPVAEEVDVDYVSTRPGM
                     MHACGHDLHMAGLVGAARILHELRDDLVGDVVLMFQPAEEGPGGAEPMIREGLLEAAG
                     RRVDVAYAVHVYSADHPFGTWFGRPGTLMAAADEVHVVVRGVGGHGSAPDRAKDPVPV
                     ACEIVLAYQSAVTRQFSVWDPVVLTVGRIAAGTKDNIIPDDAFVDATLRTFSAENRSR
                     AHDVVTRVAEHVAQAHGMTAEVTIGNGYPVTVNDVDEFAFSQGVVVDLFGADRWVDMV
                     NPEAGSEDMSFVLDEVPGAYLNVSACAFPDVADAEDNHSPRAAFDDSVVPDIAAALAE
                     MALRRTRELSRGVVADA"
     gene            complement(11045..11117)
                     /locus_tag="PL014_02995"
     tRNA            complement(11045..11117)
                     /locus_tag="PL014_02995"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11082..11084),aa:Arg,seq:tct)
     gene            complement(11144..11749)
                     /gene="thpR"
                     /locus_tag="PL014_03000"
     CDS             complement(11144..11749)
                     /gene="thpR"
                     /locus_tag="PL014_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOT56090.1"
                     /translation="MRLFVALHPSLEAAEHLEDFLDAREGADPDLRWASPAQWHVTLA
                     FMGSAPERVVDDLIEGLAAVAQRCAPPLLRIAGGGCFPDVTAARVLWAGLAGGEALAP
                     LARSVRSAAAVVGAAPEGGPFRPHLTLGRFGRPTEATRWVRVLDAYTGPTWTASEVVV
                     VASHLPRERGHRPRHEVLARCGLGGARPPGAHRDGAVPDLH"
     gene            11819..13540
                     /locus_tag="PL014_03005"
     CDS             11819..13540
                     /locus_tag="PL014_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOT56091.1"
                     /translation="MIVDSDVEALASVVEALRDEVGQTSLPLEVPSRAAAEASRTALL
                     HQLDDYVLPRLRAIDAPLLAVVGGSTGAGKSTLVNSIVGARVSRPGVLRPTTTSPVLV
                     HHPDDRGWFADARILPGLARVTGDGNPDQAGLDQPGTVRLVESSTLPAGMALLDAPDI
                     DSVVSANRAIAAQLLSAADLWLFVTTAARYADAVPWDLLRTAADRGTSVAIVLDRIPA
                     EAIDEIRPHLATMLREQGLPTAPIFTVPEAPLDADGQLPPEAVERLSAWLHALASDSR
                     ARSIVVGQTLRGAVDSLSGRTSDLVVASRDQHDAATALLRAVDESYDEAHRRVDDGMT
                     DGTLLRGEVLARWQEFVGTGEFFRQVETTVSRWRDKLTAAVKGSTPPADGLGEALQSG
                     VATLILANAETAASQSARAWRRLPGGEPVLARHPDLAKPSPGLPADVERLVRQWQGEV
                     LDLVRSEGQGRRTNARIAAYGVNAIGLFLMLVAFSSTGGLLGAEVGIAGGTAVLAQRV
                     LEAIFGDQAVRTMATKARRRLLELADELYEGEQQRYAVALPETPALADQAGRLSAVAA
                     DLRKVVR"
     gene            13537..15207
                     /locus_tag="PL014_03010"
     CDS             13537..15207
                     /locus_tag="PL014_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOT56092.1"
                     /translation="MSPLRMGRARGVVISADELSAAAAALAAAADAGGAQLPADERGR
                     ADAVVAKVAERTALVGDHTIVALAGATGSGKSSLFNALVGTDVAVTGVRRPTTATPTA
                     AIWGAQPAGELLDWLGVGARHVVGDQRDGEPEVLGSLDGLVLLDLPDFDSREVTNRVE
                     AERVLDLVDLFVWVTDPQKYADARLHDDYVSALATHEAVTMVVLNQSDRLSDADVDQC
                     RTDLVRLMERDGVPGATVLATSVATGHGIDVLRQRLANAVAGRTMARSRLAADVRAAA
                     GALTSHVAEGEPVLDDAARSDLVDALARSAGVPTVVEAVARDYRMEAVSHTGWPFTRW
                     AQSLRPRPLRRLRLDGRDISVSEADVRSVLGRSSLPPASPAARSAVEIATRRLVDRAA
                     EGLPAPWAEAVETAAAPSGPALGDALDQAVVGTPLHARSPVWWRAFGVGQLVLAAAAV
                     VGFLWLALYVVLGWLQMDAVIGAPPSIGILPVPLVLFLGGLVAGVLLALVARPLARVG
                     SQRRAQVIDRRLRESIDEVAREEIVRPVERVLERHAATRLALSRAAGN"
     gene            15340..15834
                     /locus_tag="PL014_03015"
     CDS             15340..15834
                     /locus_tag="PL014_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT56093.1"
                     /translation="MNETYVTIRGRLVADPTVRTTKTGSPMATFRVATSARKQVQGQP
                     GVWEDTPTSFYSVTTFRALAANVGVSLRKGQPVTIHGRQHITSYVREDGTTWWDVEIV
                     ADAAGHDLTYGTAAFAKVGRPGEVEGAAARPESIADEPVGDDGEDTYLNGGPLVVPAD
                     EFAA"
     gene            15893..17575
                     /gene="ettA"
                     /locus_tag="PL014_03020"
     CDS             15893..17575
                     /gene="ettA"
                     /locus_tag="PL014_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOT56094.1"
                     /translation="MPEFIYVMSRARKAHGDKVILDDVTLSFLPGAKIGVVGPNGAGK
                     SSVLKIMAGLDQPSNGEARLTPGYSVGILMQEPELNEGKTVLGNVEEGAGEIKAKLDR
                     YNEISVLMADPDADFDALMAEMGQLQEAIDAADAWDLDSQLEQAMDALRCPPPDADVT
                     VLSGGERRRVALCKLLLQKPDLLLLDEPTNHLDAESVLWLEQHLAAYHGAVVAVTHDR
                     YFMDNVAQWILELDRGRAYPYEGNYSTYLEKKQARLEVQGKKDAKLSKRLAQELEWVR
                     SNAKARQTKSKARLARYEEMAAEADRTRKLDFEEIAIPPGPRLGSKVIEVKNLRKGFG
                     DRVLIDGLSFTLPRNGIVGVIGANGVGKTTLFKTIVGLEEPDGGVVDVGETVKISYVD
                     QSRGGLDPEKSLWETVSGGHDYINVGQVEIPSRAYVSQFGFKGPDQQKKAGILSGGER
                     NRLNLALTLKEGGNLLLLDEPTNDLDVETLGSLENALLDFPGCAVVISHDRWFLDRVA
                     THILAYEGDDENPAKWYWFEGNFQAYEENKIERLGANAARPHRVTYRKLTRE"
     gene            17572..18555
                     /locus_tag="PL014_03025"
     CDS             17572..18555
                     /locus_tag="PL014_03025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT56095.1"
                     /translation="MTGPHQVSVVIPVYQGERTLAAVVEELAASVAIRVSPGGRQYQV
                     AEVLLVHDCGPDASDDTIRHLCAEHDWARPVWLSRNFGQHAATLAGIAASGGEWVATL
                     DEDGQHDPEALGSLLDTAMDQQADVVYAKPTNEPPHGPVRNLASRGAKAVVERLTKGA
                     QASDFNSYRLILGEVARSVAAYAGPGIYLDVAISWVARRAVTSPVEMRREGDRPSGYS
                     MRTLVSHFWRLVLSSGTRPLRWVSVLGATAVLLAPVIALVLLIGKALGSWDAPGWTST
                     MILLMVATGVILFSLGVIAEYVGMAVNMAMGKPLYLPVRDRRDGPLGRDAR"
     gene            18552..19514
                     /locus_tag="PL014_03030"
     CDS             18552..19514
                     /locus_tag="PL014_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT56096.1"
                     /translation="MTTRPTQPLTLVVGAGGLVGRHLVTAHGRARHEVRVAAVPWGDE
                     GESISALLLALDDFSQARQGRPWSVAWCAGAGVVATAEDALAAEVRVFDRVMGALAAS
                     VGPDEDGVVFLASSAGGVYAGSTGAPFDERTDAAPLVPYGRAKLAMEDTVQRVASETG
                     VRAVIGRLANVYGPGQTLGKPQGLLSQLCLADATGRPLPLYVSMDTIRDYLYVGDAAT
                     MIVRCLELVRGEPRGAVVTKVLSSGRPVTVGYLVSEARRVFHRPLRTIAVGGAGTGQV
                     LDLRLASRVWPDVDALASTPLAAGLSRTAADVRARVVSGGPLDP"
     gene            complement(19552..20292)
                     /locus_tag="PL014_03035"
     CDS             complement(19552..20292)
                     /locus_tag="PL014_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT56097.1"
                     /translation="MKSHARSAHGHGGSPSIEVPDYWWYVARADLLETALRSHVQGSG
                     LTLDLGSADGPSASWLRDAVSRTVALDIDPRGLGSDGVCGSALALPFADETFEVVSAF
                     DVIEHCDPESQALAEVRRVLKPRGRFVMSVPAYTWAWSDFDVANGHHRRYTRQRARSA
                     VEAAGFSVQRVTHGFASVFPMFAAERAARKLRGRRAQGAVDIVEVPTVPKPLNDALLA
                     LSRFDRHILSRADLPFGSSVFLAATRVG"
     gene            complement(20368..21048)
                     /locus_tag="PL014_03040"
     CDS             complement(20368..21048)
                     /locus_tag="PL014_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT56098.1"
                     /translation="MPSTTPLSESALLDALAPAGGRQSAVGDVSDTAVRIMLAAADAF
                     AQRGFHATTTRDIASRAGLSPAGVYVHFASKEVLLFELSRRGHERARDLLVEAAAAAA
                     NPTEALRAIIGGFSRWHAEHYELGRIVQFEFRHLRSEHRDAVLGLRKEIDQVVAGVLR
                     DGVETGEFAVTDVPATALALLSMAIDVARWYTPDVRRTPAAIEATYGELAVRLVRPGE
                     ALAASALP"
     gene            21136..22281
                     /locus_tag="PL014_03045"
     CDS             21136..22281
                     /locus_tag="PL014_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT56099.1"
                     /translation="MPRNIYGPEHEDFRASVSEFVERTLKPRAEQMLEVKSIDRDIWK
                     EAGSQGLFGLDIPEEFGGMGAEDYRFNAVSAEVINGFNAAVGSCFGIHSDVCPPYIVD
                     LGTQEQKERWLPAMAAGEKICAIAMTEPGGGSDLAALKTNAVRDGDDWILNGSKTFIT
                     NGYQADLVIVATRTDPTKGAKGITLFMVEDGMEGFTRGRKLDKVGQEESDTAELFFQD
                     VRVPDSNRIGDEGMGFIAMMQRLPQERVGAAAANVAHAKQILLETIEYAKERKAFGQA
                     IGSFQHNKFKLAEMVTAIEVAEAYVDDCIEAHAKGELTAVDAAKAKWWSAQVQNDVLD
                     ECVQLYGGYGFMNEYRVARAWRDARVTKIWAGSNEIMKELIGRDLGL"
     gene            complement(22315..22989)
                     /locus_tag="PL014_03050"
     CDS             complement(22315..22989)
                     /locus_tag="PL014_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOT56100.1"
                     /translation="MRARTPLVGGVVGLFVTVAVLHLGAQLTGAGEVADATQVLLMPL
                     LAAALWSNTEAPRSRLITLTLVALGLSWLGDSAPKLAEGDTAFLIMIGFFLLAQVAYV
                     MAFWPFRSRSVLHVRRPVLLGYVAVVVALVLACVGGAAGMLVPVLVYGTLLGTMAVLA
                     TGVNLPTAIGGALFLVSDGLIALNAFAEGFDLPGQGFWVMATYLAAQSLIVGGVLTTR
                     DAARSR"
     gene            complement(23007..23762)
                     /gene="fabG"
                     /locus_tag="PL014_03055"
     CDS             complement(23007..23762)
                     /gene="fabG"
                     /locus_tag="PL014_03055"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOT56101.1"
                     /translation="MENQRVAVVTGAARGIGAATATRLARDGFAVAVLDLDEAACEAV
                     ADAIRAEGGTALGLGVDVTDAEQVESGVARVADELGPPVVLVNNAGIIRDNLIFKMTE
                     GDWDAVMAVHLKGAFLMTRACQAHMTAQKYGRIVNLSSSSAQGNRGQANYSAAKAGLQ
                     GFTKTLAIELGRFGVTANAVAPGFIQTDMTAATAERIGVPFEEFLAHAASQVPVQRVG
                     QPEDIAATIAFLVSQEAGFVSGQVIYVAGGPLD"
     gene            23925..>25163
                     /gene="glgX"
                     /locus_tag="PL014_03060"
     CDS             23925..>25163
                     /gene="glgX"
                     /locus_tag="PL014_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOT56102.1"
                     /translation="MQIWPGTAYPLGATYDGSGVNFALFSEVAERVELCLIDDEGVET
                     RIDLPEVDGFVHHAYLPGVSPGQLYGFRVHGPFDPANGHRCDPSKLLLDPYAKAIEGQ
                     VTNDQALFSYDFTDPTKRNTEDNLGKTMLSVVINPYFDWGHDRPPRHEYHDTVIYEAH
                     VKGLTMTHPEVPEEIRGTYAGIAHPAIIDHLSTLGITAIELMPVHQFVQDTSLQAKGL
                     TNYWGYNTIGFLAPHNGYARGRRGEQVTEFKAMVKALHAADIEVILDVVYNHTAEGNE
                     FGPTLCFRGIDNAAYYRLVDDAKEHYYDTTGTGNSLLMRHPHVLQLIMDSLRYWVTEM
                     HVDGFRFDLAATLARQFHEVDKLSAFFDLIQQDPVISQVKLIAEPWDVGDGGYQVGNF
                     PPLWTEWNGKYRDTVRDFWRG"
BASE COUNT         3682 a         8973 c         8818 g         3690 t
ORIGIN      
        1 gaggtgatgt tctacgtcga cggcgactac gaggcccgca aggggtcggg catcgccaag
       61 ggctcgatct ccctgcaccc cggggggcac gcccacggac cccagcccgg tgcggccgag
      121 gcgtcgatcg gcaaggagtc cttcgacgag ctcgccgtca tggtcgacac cttccgcccg
      181 ttggagatcg gtgagggtgg ccgggcctgc gacgacggcg tgtacgccgg gtcgtggaac
      241 cgcccccgcg cctgagtgtg gcgcgagggt aggctggccg atcatcctgg cccgtcgcgt
      301 gcgtggcccg caccgcagga ggggccaccg cgtgagtgac gtgctgaccc tgcacgggct
      361 ggggtgctcg gtcgaggtcc ggtgctccgg tgacgcggcc gcccccctgc tggaggcgtt
      421 gcgggcggcg tggtcacggt gcctcgtcgc cgacggccgc ggcgagcccc gggcggccaa
      481 cccggtcgag gcgcggctcg acgacgaggg tgacctcgcc caccggctca tggccacgac
      541 ccagacggtg acccgggcgc tgatcgccgc ccaggcgggg cacctgctca tggtgcacgc
      601 cggagcggtc ggccacccgc tgaccggggt cagcctcgtc tacgtcgctc ccggcggcac
      661 gggcaagacc acgctgagcc ggcgcctcgg gcagtcgtac ggctacctca ccgacgagac
      721 cgtcggcatg gatgccgacg gccggatcct gccctacccc aagccgctgt cggtgcgtcg
      781 gcaggagcag ccgggcgtca aggacgagct gtcgcccgac gccctcggcc tgctcgccgc
      841 gccgccggcg ccggtggcgt ccagggtcgt gctgctggcc cgggaccctg ccgccacggc
      901 cgccgacgtc gaggaggtgc ccttcatgga tgccgtgttc gccctcaccg agcagtcgtc
      961 ctcgctggcc gcgctgccac ggccactgca ccggatggcc gacttcatcg acgcggccgg
     1021 ccccgtgctg cgccttcgct acgccgaggc cgccgacctc gacggcacgt tgaccgccct
     1081 gctgggtggt gcggcatgaa ggtgcagcgc tgccccgtcg acgacctgct gacccgcgac
     1141 ggcgagaccg ccgtgctcgc ccacggcaag gcactgcggc tcagcgccgt cgccgtcgcg
     1201 atctacgacc tcaccgagca cgagatcgag gtggagtccc tcgccgaggc cctggagtcc
     1261 caggtgggag cccctgcgga ccgctcgacc ctggaggcga ccgaggcggc ggtcgcgatg
     1321 atggtgcgcc acggcatcct cacgcggtcc tcacgctgag caccgcgaag gtgcggccta
     1381 tccttgcgag gtgtcgctcg cacccgtcac gcaggaggtc accgccaccg gcggtgacct
     1441 cgcccccgac ctgccgtggg tcacgatcgt gtggaacgac ccggtcaacc tcatgtccta
     1501 cgtgacctac gtgctgcaga gccacttcgg ctactcccgg gccaaggcgg agaagctgat
     1561 gatggacgtg cacacccagg gacgcgccgt cgtcgcgaac ggcacccggg aggccatgga
     1621 ggccgacacc gaggcgctcc agggctacgg cctctgggcg acgttccaga aggactcctg
     1681 atggcgcggg ggttccgccg caaggggcgg gggagggccg cgtcctacgt cgcacgcctc
     1741 gacccgctcg agcgggcgac cgtcgccggg ctgatggagc aggtgcacga cctgctcgag
     1801 ccaccgccga cgacgaccgt cgggcccggg tcggaggggc cggtggacga gttcgccgcg
     1861 atcgtcgccg gcctggggat gggtgtcacc gtcgcggccg aggaccaggc cgacctcgtg
     1921 gcctccgggg ccggccgcga ccccgcgctc gaccgcctgc tgccgacggc caaccgccag
     1981 gacgacgagg ccgcggcgga gttccgccgg ctcaccgaga ccgggttgcg ccagcgcaag
     2041 gcagcaggcc tcgcgctggc ggcgagcctg ctccgtgtcg aggacaaggt cgtgctggac
     2101 gaggcctcgg cgcgtgcctt cctcaccgcg ctcaccgatg tccggctcgt cctcggcgag
     2161 cggatgggcc tgcgcaccga cgaggacctc gaggccctcg aggcggcggc cggcgagctc
     2221 gacccggacg acccgctcgg gtacgccctg gccgtgtacg acttcctcac ctggttacag
     2281 gagaccctgg cctcggccat gctgacggcc tgagcgaccg ggtcccggcc gcctcggtcg
     2341 tgcgtacccg cccgtcaccg ggcgactgcg agggcgctgt gacgcgtgcg acgtgccgac
     2401 ggcgatgtgc cggtgggcgt gggcgcatac cctttgcggg tggctgacca accgattggg
     2461 gtgttcgact ccggctatgg cgggctgacc gtcatgcggg cggtcctcga ccagttgcca
     2521 cacgagtcgg tggcctactt cggcgacacg gcgcgcgccc cctacggccc tcgtcccatc
     2581 gcccagactc gtgagctcgc gctggagtgc ctggaccggc tcgtcgacca cggtgtcaag
     2641 gcgctcgtca ttgcctgcaa cacggcgagt gcagcggtgt tgcacgatgc ccgtgaacgc
     2701 tacgacgtcc cggtcatcga agtgatccga cccgcagtgc gccgcgcggc ctctgccacg
     2761 cgcaatggtc ggatcggtgt catctcgacc gtcggcaccc accagtcggg tgcctacctc
     2821 gacgcgttcg ccgcagctcc gcacctcgac gtcgtgagca cgccgtgccc gcgcttcgtc
     2881 gagttcgtcg agcggggcat caccggtggc tcggagctgg tcgcggtcgc gcacacctac
     2941 cttgatccgt tgcgtgagca gggtgtggac accctcgtgc tcgggtgcac gcactatccg
     3001 ctgctcgcgg cggtgatcac ctatgtcatg ggcgacggca tcacgttggt ctcgtcggca
     3061 gaggagaccg ccaaggacgt ctatcgcgtc cttgccgacg cagacctcct gcgacccgat
     3121 ggactcccgc cacccgacca cgggttcacg accacggggg atccggacga gttccgccgc
     3181 ctggccgccc gtttcctggg tgtcggctca cgcttgggcc cggtgttcac cgccccgctg
     3241 cccctcgacg tcgaggggcc cgagtgagag tcaccgtcat cgggtgctcg ggctcattcg
     3301 ccggcccgag ctccccagcg tcctcctacc tggtgcaggc ggagttcggt gggcggacgt
     3361 ggagcatcct gttggatctc gggagcggag cgctcggggc gctgcagcgt cagatgtccc
     3421 ctgacgacct cgacgccgtg ctcatcacgc accttcaccc tgatcactgc gtcgacctct
     3481 gcggcctcta tgtctcgcgt cgctaccggc ccttcggccc gttgccggca cgcctacccg
     3541 tcttcgggcc gaccggaacg gcacgacggc tgcagctgat gtaccacggc ctcgagcccg
     3601 gtgggatgga ggccgagttc gacatccaca ccgtggtgga tgcagcaccg atcctgatcg
     3661 ggccgttcac cgtgactccc tatcgcgtga accaccccgt cgaggcgtac ggctaccggg
     3721 tcgaggcggg gggcgcactc ctggcctaca ccggcgacac ggacgactgc cccaatctcg
     3781 accccctgct gcgcggtgtc gatcttgcgt tgatggactc ggccttcatc gatgggcgcg
     3841 acgacgtcgt cgacatccac ctgtcggggt cccgcgccgc cagggccgca ctgcgcgcgg
     3901 gtggtgtgca gagcctggtg ctgacgcaca tcccgtcctg gaacgacccc gaggtctgcc
     3961 gtgctcaagc ggccatgcac tggccctctg ccgtggggct cgccgctgcg gatgcggtct
     4021 acgagatctg agcgtccccc gtgcgcgcgg acctcacggg caaccgtgcg gccgcccgcg
     4081 gctcgacacc gcccgcggtg gctgatctgc cccccgtgac gccatagcct tgggtccatg
     4141 ccttctgact caattcgcca tgatggtcgt gccccggatc aacttcgtga ggtgcggatc
     4201 acccgcaact ggctcgacca cgccgagggc agcgtgctcg tggagttcgg gcgaacccgc
     4261 gtcctgtgcg cggcctcgtt caccgcaggg gttcctcggt ggctcaaggg ccggggtacc
     4321 ggctgggtca ccgctgagta cgagatgctg cccaggtcga ccaacacccg ctcggaccgg
     4381 gagtcacgcc gggggaaggt cggcggacgc acccacgaga tcagccggct cgtcggccgc
     4441 agcctgcgcg cggtcatcga caccaagggc ctcggcgaga acaccatcgt cctggactgc
     4501 gacgtcctcc aggccgacgg aggcacccgc acggccgcga tcaccggggc gtgggtggcc
     4561 ctggcggatg ccgtgtccga tgcccggtcc cggggcctga tcccaccgtc cgcccagccg
     4621 ctcaccgggt cgatcgcggc tgtgagcgtc gggctcgtcg acggactccc ggtgctcgac
     4681 ctcgactacc cggaggactc gacggcgcag accgacatga acgtcgtcat gaccggggac
     4741 gggcggttcg tggaggtgca gggcaccgcc gagggggagc cgttcgaccg cgccgtgctc
     4801 ggcgacctgc tcgacctcgc gaccgtcggc tgcgcggagc tcaccctcct ccagcaggca
     4861 gccctggcgg cgacgccgcg ggagcgacgc gcgtgagccg tcggctggtg ctggccaccc
     4921 gcaacgagca caaggtgcac gagctgcgcc agatcctcgg cagcctggtc accgacctcg
     4981 acctcgagat catcggggcc ggcgacgtgg agggcgcccc ggacgtcgcc gagaccgagg
     5041 tgacgttcct cggcaacgcc cggctcaagg ccgtggccct ggccgaggcc accggcctgc
     5101 ctgcgctcgc cgatgactcc ggcctcgccg tcgaggtcct cggtgggtcc ccaggcgtgt
     5161 tctcggcccg atggtcgggc tcggccgccg ggccgggggc cacgcgggcg gaggtcgaca
     5221 gggccaacct gcacctgctg ctggagcagc tcggcgacgt ccccgacgag caccgggccg
     5281 cggccttcgt gtgcgccgcc gtcctcgcga tgcccgacgg tcgggtccac ggggtcgagg
     5341 gacgcgtgga gggccggctc gcccgcgccc ccgaaggcca ccacggcttc ggctacgacc
     5401 ccgtgttcgt cccgaccggg cagcagcgca cgctcgccga gcacaccgac gaggagaaga
     5461 acgcgatctc ccaccgggga cgggccttcc gcgccctcga gccggtgctg cgagaactcc
     5521 tcggcggctg agcgccgttc gtcgtaggct gtcgaccgcg cgcgagtggc ggaactggta
     5581 gacgcgccag atttaggttc tggtgccttc gggtgtgcgg gttcgagtcc cgccttgcgc
     5641 accaggtccc gtgtggttcg tcggcccgcg gttccgttgg cccggggccg atgtaggttg
     5701 gcaccaccga tgcacgacac gacgaggagg caccgtgggt gaccggctca gccccggcga
     5761 cagcgcgccc gacttcaccc tgcccgatga caccggcacg ccggtgacgc tgagcgcgct
     5821 gcgcggcggc aaggtcatcg tctacttcta cccggcggcg atgacgccgg ggtgcaccac
     5881 gcaggcgtgc gacttcagcg agtccctgga gtccttgggc gcgcacggct acacggtgct
     5941 cggcatctct ccggacacgc cggagaagct tgcccgcttc cgcgagaagg acgccctgac
     6001 gatcaccctg ctgtccgacg cggaccgggc cgtcatgacg gcgtggggtg ccttcggcga
     6061 gaagaagctc tacggcaaga cggtccaggg tgtcatccgc agcaccgtcg tcctcgatga
     6121 gcagggcatc gtgacgcacg cgttctacaa cgtcaaggcc accggacacg tcgccaagct
     6181 gcggcgcgac ctcggactgc agtgaggaac ccatgagcaa cgtccagcct gcggccacgg
     6241 ggccctcgac cggggccgcg gcacccgcac cggtgaccat cgccgacctc gaggccgaga
     6301 tcaccgcgcg tcgtgaccgg ctcgcgcaaa ccatcgacga gctcgccgag cgcgtgcgcc
     6361 cgtcctcgat catccagcgc cagaccgacg tcgccaagca gcggttcgcc gacgtcgcga
     6421 ccacgccgtc gggggagctg cgcgtggagc ggctggccgc ggtggtggcc gtggcggcgg
     6481 tcgtgggcag ctggctgatc gtgcgccggc tgcgtcgcgg ctgagcgtgg tcgtggcctc
     6541 gagggcggcc acggggcgcc tgcccgtccg catgctgcac gaccgggagg cgagtcggtc
     6601 tccgcgacgg gcagacgtcc gggaacagcg aacgaggccc cccggtgtcg gggagcctcg
     6661 ttcggtccag cgtggtgcgc catcagggac tcgaaccccg aacccgctga ttaagagtca
     6721 gctgctctgc caattgagct aatggcgcgc gtcgcagaca ttagcaggat ggcccgcgac
     6781 ccgcgaaatc gcggtggcga agcggtgccc ggctcagtcc cgcaggtcat ccggccggct
     6841 gcggcggcgg tgccacacga gtcccaggac ggcggcgagg gcggcgacac ccacggcgat
     6901 gccgaccccg acccacggcg acgtgcccgc ctcgtcggcc acgggctcgg gggtggttgt
     6961 gggggagtcc gatggcatgg gcgtcgcgga tgccgtgggg acggtgggcg tcgggctcgc
     7021 ctccggggtg ctcgtcgggg tgctcgccgt cggggaggca gtggccgcga ccgccatcga
     7081 cgtgaacgtc accgtcccgg agatcgggtg cccgtcgttg ctcaccaccc ggtaggtcac
     7141 cgtgtgctcg cccgccgcca gtggcgccgc gaggggctgc gtcacggtgg cgccctcgac
     7201 gctcggcgcc ccgtcgacct cggccccctc gggtccgctc acccggacgg cgacgaagtc
     7261 ggggttgatg ctctcgttga aggtcagcac cacccggtcc gcggtctcga cgctcgtccg
     7321 gtcggccggc tccgaggaca ccaggcgcgc gtggaacggc agggttccgg gcgcagggga
     7381 ggcgaaggcg ggcgcgatgg cgacgaggaa gaacgcgacc acgatgacga gcgtgaccac
     7441 ggtggcgaag cgacgggatt gcatggggcc catcctccca aagcctgcgc gcggatccgg
     7501 tcgcaggtcc ggtgacatcc ccgaggactg caaaaccccg acccgccgta gcggatcggg
     7561 gcctcgcagt gcctggggtg agtaacggga cttgaacccg cgaccctcgc gaccacaacg
     7621 cgatgctcta ccagctgagc tatacccacc acgacgggcg cgacggcatc cgaagagcct
     7681 gtgcgcccgt agcgcgcaca atcgtacctg ttacgagagg gtgctctcga ccacggtccc
     7741 atccgtcggg gcagctgccg tcgaggcggc ctcgtggtcg acgacgtggc gcgccgccag
     7801 ggcgcgcgcc tggtcggtcg agggccccgg cttcgccacg aacaccgcct cgcggtagta
     7861 ccgcagttcg gcgatggact cgcggatgtc ggccagagcc cggtggcctc cggacttgct
     7921 cggcgccgcg aagtaggccc gggggtacca gcggcgcgac agttccttga tcgaggagac
     7981 gtcgatgatc cggtagtgca ggtggctctc gagctcgggc atgtcgcgag cgaggaaggc
     8041 ccggtcggtg gcgacggtgt tcccacccag tggtgacttg cgcgcttcgg gagcccactc
     8101 gcgcacgtag tcgagcacca tctcctgagc ccgctccagc gtggtgccgg cagcgagcac
     8161 gtcgagcagc ccggacgtcg tgtgcatgtc ccgcacgaag tcgtccatct ggtccagtgc
     8221 ctcgtcgggg ggccggatga cgacgtcgat gccgtcaccg agcaccgtca gctcgtagtc
     8281 ggtgacgagg gcagcgacct cgatgagggc gtcactggtg agggacagac cggtcatctc
     8341 gcagtcgatc cagacgatgc ggtcgctcag ggctcggtct gtgcctgggg ttgccatgac
     8401 cgccatgcta ggcgcgcctg ttgcacgccg ctgcccgatc gtcccagccc atcctcggct
     8461 agggtcgcgt gccatgagcg gtgacctgtc gacgaggccg cccgacctgc gggtgcgccg
     8521 caatccgacg gcgcgcggtg ccctgcgcgg ttgggtgctc accggcatcg ccggactggg
     8581 gttcctgatc gtggccctgg tcgtcgccgc gtacttcgat gccgccttcg gcgtccaggc
     8641 cagcctcctg gcgctcgcgg ctgcggtcgt gcccctcggt atcgtgatcc cgaccttcct
     8701 gtggctcgac cgcttcgagt ccgagcccaa ccgtctcctc gtcggcgcat tcctgtgggg
     8761 tgccctcgtc gccgcggtgg tgtcggccct gctcaacacc accgccatgt ccctcatcga
     8821 ggccatgtcc accgccgacc cggacgccgc gctgaccacg acggcggtgc tcgttgctcc
     8881 gttcgtcgag gaggccgcca agggggtgct cgtcctggtc gtctggtggt tcctgcacca
     8941 cgagttcgac ggcatcaccg acggcatggt ctacgccggc atctgcgcgg ccggcttcgc
     9001 cttcaccgag aacatccagt acctcgcgca ggcctggacc gagggcggta gcgaactgct
     9061 caccggcacc ttcatcgccc gctgcctgtt ctcacccttc gcccacccga tgttcaccgt
     9121 gctcaccggc atcggcatcg ggatcgcggc gaccagccgt tcgtggatgc cccgcctgct
     9181 cgcgcccgtc gccggcttcc tgctcgccgt gctcagccac gggctgtgga acctcgccgc
     9241 cgtcacgggc ggcgagggca tggtcgtcat ctacctgttc gtccaggtgc cgatcttctg
     9301 tgccttcctc gccttcgtcg cgtgggtgcg tcgcacggaa ggacacctca tcggacagtt
     9361 cctgcggccc tacgccgacg tcggatggct ctcgccggcg gaggtcgcca tgctctcctc
     9421 gatgccgcag cgccgcgagg cgcgtgcctg ggcgcgggcc aacaccggtc gcgaggggct
     9481 gaaggcaatg cgggcgttcc aggatgccgc gagcgagctc gcgctgttgc gtcgccggat
     9541 gagccaccgg gcagccgatg cccatgccat cgagcaggag cacgaactgc tgctcgccct
     9601 gcgcgaccgg cgggcggagt tcatcggcct gccggcttcc tgatccctgc cccgaaaggt
     9661 cgacgtgact gacaccctgc gcattgctgc ccgcctggcc ccggagttgg tggcgctgcg
     9721 gcgccacctc cacggcatcc ccgaactggg cctggacctg cccgacaccc aggccgccgt
     9781 gctcgaggcg ttggcggggc tcgacctcga ggtgaccctg ggggagtcgc tcagttctgt
     9841 cgtcgcggtg ctgcgcggcc gtgcaccggt cacgggggag cggcccgtcg tgctcctgcg
     9901 cggagacatg gacgccctgc ccgtggccga ggaggtcgac gtcgactacg tctcgacccg
     9961 ccccgggatg atgcacgcgt gcggccacga cctgcacatg gctgggctcg tcggcgcggc
    10021 ccgcatcctg cacgaactgc gcgacgacct cgtcggtgac gtggtgctga tgttccagcc
    10081 tgccgaggag gggccgggcg gggccgagcc gatgatccgc gagggtctgc tcgaggcagc
    10141 cggacgccgc gtcgatgttg cctacgccgt gcacgtctac tcggctgacc accccttcgg
    10201 gacgtggttc gggcggccgg gcaccctcat ggcggccgcg gacgaggtgc acgtggtcgt
    10261 gcgcggggtg ggtggccacg ggtcggcccc ggaccgcgcc aaggaccccg tgcccgtggc
    10321 gtgcgagatc gtgctggcct accagagcgc ggtcacccgc cagttcagcg tgtgggaccc
    10381 cgtggtgctg acggtgggga ggatcgcggc cgggacgaag gacaacatca tcccggacga
    10441 cgccttcgtc gacgcgacgt tgcggacctt ctcggccgag aaccggtccc gcgcccatga
    10501 cgtcgtcacc cgggtcgccg agcacgtcgc ccaagcccac gggatgaccg ccgaggtcac
    10561 catcggcaac ggctaccccg tgaccgtcaa cgacgtcgac gagttcgcgt tctcccaagg
    10621 cgtcgtcgtc gacctgttcg gtgccgatcg ctgggtcgac atggtcaacc ctgaagcggg
    10681 gtcggaggac atgtcgttcg tcctcgacga ggtgcccggt gcctacctca acgtgagcgc
    10741 gtgcgccttc ccggacgtgg ccgacgcgga ggacaaccat tcgccgcgag cggcgttcga
    10801 cgactcggtg gtgccggaca tcgcggcagc cctggccgag atggcgctgc gccggacacg
    10861 tgagctctcc cggggcgtcg tcgctgacgc gtgacgcccc aaaggcgcac cccggggagg
    10921 ccgtgaggcc tcaacccggg gtgctgggcc ctcgcgcact ggggggagag aacgagggcc
    10981 tgctcgaagt caaccatgct tgcggcgatt gcagccactc ccgtcccgga actgtccaac
    11041 tgagcgcccc cggcaggact cgaacctgcg acctcgagat tagaaggctc ttgctctatc
    11101 cgctgagcta cgggggccga gcgatcatcc cgaccgccga aggtcagtgt aggtctggca
    11161 cggcaccatc gcggtgggcg cccgggggtc gtgcaccgcc gaggccacag cgtgccagga
    11221 cctcgtggcg tggacggtgc ccacgctccc gggggaggtg cgaggcgacg accacgacct
    11281 cggatgccgt ccacgtcgga cccgtgtagg cgtccagcac ccgcacccac cgggttgcct
    11341 ccgtgggccg cccgaatcgg cccaaggtga ggtggggacg aaacggcccg ccctcggggg
    11401 ctgcgccgac cacggcggcg gcgctgcgca ccgagcgtgc caacggtgcc aaggcctccc
    11461 cgccggccag ccccgcccac agcacccggg ctgccgtcac gtccgggaag cagcccccac
    11521 cggcgatgcg caggagcgga ggggcgcagc gttgcgccac agcggcgagg ccctcgatga
    11581 ggtcgtccac cacccgctct ggcgctgacc ccatgaaggc gagcgtcacg tgccactgcg
    11641 ccggcgaggc ccacctcagg tcggggtcgg ctccctcccg ggcgtcgagg aagtcctcga
    11701 ggtgctcggc cgcctccagg ctcgggtgca gtgccacgaa cagtcgcata ctgaacatca
    11761 tccggcgcgc ccgaggcaat cgcctgtcga cctgcacaaa gtcgttaggg ttggcgtcgt
    11821 gatcgtggac agtgacgtgg aggcgctcgc ctctgtcgtc gaggccctgc gcgacgaggt
    11881 cggtcagacg tcactgccgc tcgaggtgcc gagccgagca gccgccgagg cttcgcgcac
    11941 ggccctgctg caccagctcg atgactacgt gctcccccgg ctgcgcgcga tcgacgcacc
    12001 cctgctcgct gtcgtgggtg gatcgacggg cgcgggcaag tcgaccctgg tcaactccat
    12061 cgtcggcgcc cgggtgagcc gtccgggcgt tctgcggccg acgacgacgt cgcccgtcct
    12121 ggtgcaccac ccggatgatc gcggctggtt cgccgacgca cgcatcctgc ccggcctcgc
    12181 gcgggtcacc ggggacggca accccgacca ggcggggctc gaccagccgg gcacggtgcg
    12241 cctcgtcgag tcctcgacgt tgcccgccgg gatggccctg ctcgacgccc cggacatcga
    12301 ctcggtcgtc tcggccaacc gcgccattgc cgctcagctg ctctcggcgg ccgacctctg
    12361 gctgttcgtc accacggcgg cacggtatgc ggatgccgtg ccgtgggacc tgctgcgcac
    12421 ggccgccgac cgcggcacgt ccgttgccat cgtcctcgac cggatccccg ccgaggccat
    12481 cgacgagatc cgcccccacc tggcgaccat gctgcgcgag caggggctgc ccaccgcacc
    12541 catcttcacc gtccccgaag cacccctgga tgctgacggc cagctgccgc ccgaggccgt
    12601 ggagcggctc agcgcgtggc tgcacgccct ggccagcgat tcccgggcgc gctccatcgt
    12661 cgtcggccag acccttcgcg gtgccgtcga ctcgctgtcc gggcgaacga gtgacctcgt
    12721 cgtggccagc cgtgaccagc acgatgccgc cacggctctg ctccgggccg tcgacgagag
    12781 ctacgacgag gcccaccgtc gcgttgacga cggcatgacc gacggcaccc tgcttcgcgg
    12841 cgaggtcctg gcccgctggc aggagttcgt cggcaccggt gagttcttcc gccaggtgga
    12901 gacgaccgtc tcccgctggc gcgacaagct caccgccgcc gtcaagggca gcacgccccc
    12961 ggccgacggt ctgggtgagg ccctgcagag cggggtcgcc accctgatcc tcgccaatgc
    13021 ggagaccgcg gcctcgcagt cggctcgcgc ctggcgccgc ctgccgggtg gggaaccggt
    13081 gctcgcgcgg catcccgatc tcgccaagcc gagcccgggg ctgcctgctg acgtcgagcg
    13141 actcgtgcgt cagtggcagg gcgaggtgct cgacctcgtt cgcagcgagg ggcagggccg
    13201 tcgaaccaat gcgcgcatcg ccgcctacgg cgtcaacgcc atcggcctgt tcctgatgtt
    13261 ggtcgcgttc agcagcaccg gtgggttgct cggtgccgag gtcgggatcg ccggtggtac
    13321 cgcggtgctg gcccagcggg tgctcgaggc gatcttcggt gaccaggccg tgcgcacgat
    13381 ggccaccaag gcccgtcgcc gccttctcga actcgctgac gaactctacg agggggagca
    13441 gcagcggtac gccgtcgcgc tccccgagac gcccgctctc gccgaccagg ccggacgcct
    13501 cagcgctgtc gccgctgacc tgcggaaggt cgtgcggtga gcccgctgcg catgggacgt
    13561 gcccgcggcg tcgtgatctc cgcggatgag ttgtcggctg cggccgcggc cctcgcagcg
    13621 gcggcagatg ccggtggcgc ccagctgcca gccgacgaac gcgggcgggc ggatgccgtg
    13681 gtggccaagg tggccgagcg caccgcgctg gtcggggacc acaccatcgt cgcgctcgcc
    13741 ggtgccacgg gcagtggaaa gtcctcgctg ttcaacgcgc tcgtggggac cgacgtcgcc
    13801 gtcaccggcg tgcgccgacc gacgacggcg acgccgacag cggcgatctg gggcgcgcag
    13861 ccggccgggg agctcctcga ctggctgggt gtgggcgccc ggcacgtggt cggagaccag
    13921 cgcgacggtg agcccgaggt gctcgggtcg ctcgacggcc tggtgctgct cgacctgccc
    13981 gacttcgact cccgtgaggt cacgaaccgg gtcgaggccg agcgtgtgct cgacctggtg
    14041 gatctcttcg tgtgggtcac cgacccgcag aagtacgcgg acgcacggtt gcacgacgac
    14101 tacgtctcgg cgctcgccac ccacgaggcc gtgaccatgg tcgtcctcaa ccagagtgat
    14161 cggctcagcg acgccgacgt cgaccagtgc cgcaccgacc tggtccggct gatggagcgc
    14221 gacggggtcc ctggtgcgac cgtgctcgcg acgtcggtcg ccaccggtca cggcatcgac
    14281 gtcctgcgcc aacggttggc caacgccgtg gctgggcgca cgatggcgcg ctcacgcctg
    14341 gccgcggacg tccgggccgc tgctggtgcc ctcaccagcc acgtggccga gggtgagccc
    14401 gtgctcgacg acgcggcgcg gtccgacctc gtcgacgccc tcgcgcgaag cgctggcgtt
    14461 cccacggtgg tcgaggccgt ggcccgcgac taccggatgg aggcggtgtc ccacaccggg
    14521 tggccattca cccggtgggc gcagtccctt cgcccgcgac ccctgcggcg cttgcgcctg
    14581 gatggtcggg acatcagcgt ctccgaggcc gacgtccggt ccgtcctcgg gcgctcctcg
    14641 cttcccccgg caagcccggc agcacgctcg gccgtcgaga tcgccacccg tcgtctcgtg
    14701 gaccgcgcgg ccgaggggct gccggcgccg tgggccgaag ccgtcgagac cgccgccgca
    14761 ccctcgggcc cagccctggg ggacgccctc gaccaagcgg tcgtcggcac ccccctgcac
    14821 gcccgatcgc cggtgtggtg gcgagcattc ggggtcggcc agctggtgct ggccgccgcc
    14881 gccgtcgtcg gattcctctg gctcgccctg tatgtcgtgc tcgggtggct ccagatggat
    14941 gccgtgatcg gtgcgccccc cagcatcggg atcctcccgg tgccgctcgt cctgttcctc
    15001 ggtgggctgg tggctggtgt gctcctggcg ctggtcgccc gcccgttggc ccgtgtcggg
    15061 tcacagcgac gagcccaggt gatcgatcgt cgactgcgtg agtcgatcga cgaggtggcc
    15121 cgtgaggaga tcgtccgccc ggtcgagcgg gtcctcgagc gccacgctgc cacccggctc
    15181 gctctctccc gagccgcggg aaactgagcc ctctcgtcca cagggtgggt gcccggccga
    15241 ggttgtccac aggttggccg caccagtggt ggcacctcca cgttcgggag cagtctcagg
    15301 ggagtcccga gatgcggggc accaaaggag gggttcgcga tgaacgagac ctatgtgacc
    15361 atccgggggc ggctcgtggc cgaccccacg gtccgcacga ccaagacggg gtcgccgatg
    15421 gcgacgttcc gcgtcgcgac gtcggcgcgc aagcaggtgc agggccagcc gggggtgtgg
    15481 gaggacaccc cgacgagctt ctacagcgtc accaccttcc gggcactcgc cgcgaacgtc
    15541 ggggtctcgc tgcgcaaggg gcagccggtg acgatccacg gccggcagca catcacgtcg
    15601 tacgtcaggg aggacggcac gacgtggtgg gacgtcgaga tcgtcgctga tgcggccggt
    15661 cacgacctga cctacggcac cgcggcgttc gccaaggtcg gccgccccgg cgaggtcgag
    15721 ggggctgcgg cgcgccccga gagcatcgcc gacgagcccg tgggcgatga cggcgaggac
    15781 acctacctca acggggggcc gctggtggtg cctgcggacg agttcgcggc ctgaggggtc
    15841 gccgaggcct cggattcggg ccggggcact ggtggtcgat agcctgtgcc ccatgcccga
    15901 gttcatctac gtcatgtccc gtgcccgcaa ggcacacggc gacaaggtca tcctcgacga
    15961 cgtcaccctg tccttcctcc ccggcgccaa gatcggcgtg gtcggcccga acggcgcagg
    16021 caagtcctcg gtgctgaaga tcatggcggg gctggaccag ccgtcgaacg gcgaggcgcg
    16081 gctcaccccc gggtactcgg tcgggatcct catgcaggag cccgagctga acgagggcaa
    16141 gaccgttctc ggcaacgtcg aggagggcgc tggcgagatc aaggccaagc tcgaccggta
    16201 caacgagatc tcggtgctga tggccgaccc cgacgccgac ttcgacgccc tcatggccga
    16261 gatgggccag ctccaggagg ccatcgacgc cgctgacgcc tgggacctcg actcccagct
    16321 ggagcaggcg atggacgcgc tgcgctgccc gccgccggac gctgacgtga cggtgctctc
    16381 cggcggtgag cgtcgccgcg tcgccctgtg caagctgctg ctgcagaagc ccgacctgct
    16441 gctcctcgac gagccgacga accacctcga cgccgagtcg gtgctgtggc tcgaacagca
    16501 cctcgctgcc taccacggtg ccgtcgtcgc cgtgacccac gaccggtact tcatggacaa
    16561 cgtcgcccag tggatcctcg agctcgaccg cggccgcgcc tacccgtacg agggcaacta
    16621 ctccacgtac ctggagaaga agcaggcccg actcgaggtg cagggcaaga aggacgccaa
    16681 gctctccaag cgactggcgc aggaactgga gtgggtgcgc agcaacgcca aggcccgcca
    16741 gaccaagtcc aaggcccgcc tcgcccgcta cgaggagatg gccgctgagg cggatcgcac
    16801 ccgaaagctc gacttcgagg agatcgcgat ccccccgggc ccccggttgg gcagcaaggt
    16861 catcgaggtg aagaacctgc gcaaggggtt cggtgaccgg gtgctcatcg acgggctgtc
    16921 cttcaccctg ccgcgcaacg gcatcgtcgg agtgatcggc gcgaacggcg tcggcaagac
    16981 cacgctgttc aagacgatcg tcgggctcga ggagcccgac gggggagtgg tcgacgtcgg
    17041 tgagacggtc aagatctcct acgtcgacca gagccgcggc ggtctcgacc cggagaagtc
    17101 gctgtgggag accgtctccg gcgggcacga ctacatcaac gtcggtcagg tcgagatccc
    17161 ctcgcgcgcc tacgtcagcc agttcggctt caagggcccg gaccagcaga agaaggccgg
    17221 catcctctcc ggtggcgagc gcaaccgcct caacctcgcg ctgaccctca aggagggcgg
    17281 caacctcctg ctcctcgacg agcccaccaa cgacctcgac gtcgagacgc tgggatccct
    17341 ggagaacgcg ctgctcgact tcccgggctg cgccgtggtc atcagccacg accggtggtt
    17401 cctcgaccgg gtcgcgacgc acatcctcgc ctacgaaggt gacgacgaga accccgccaa
    17461 gtggtactgg ttcgagggca acttccaggc ctacgaggag aacaagatcg agcgactcgg
    17521 agccaacgcc gcccgcccgc accgggtgac ctaccgcaag ctcacccgcg agtgaccgga
    17581 ccgcaccagg tctccgtcgt catccccgtc taccaggggg agcggacgct ggccgctgtc
    17641 gtcgaggaac tggcggcatc ggttgcgata cgggtgtcgc ccggtggtcg gcagtaccag
    17701 gtggccgagg tgcttctggt ccacgactgt ggtccggatg cctctgacga cacgatcaga
    17761 cacctgtgtg cggagcacga ctgggctcgc cccgtgtggc tgagccgcaa cttcggccag
    17821 cacgccgcga ccctggcggg aatcgccgcc tcgggtggcg agtgggtcgc gaccctggac
    17881 gaggacggcc agcacgaccc ggaggcgctc gggtcgctgc tggacacggc catggaccag
    17941 caggctgacg tcgtctacgc caagccgacc aacgaaccgc cccacggacc ggttcgcaac
    18001 cttgcctccc gcggggccaa ggcggtcgtc gagcgcctca ccaagggtgc tcaggcctcg
    18061 gacttcaaca gctatcggct gatcctcggg gaggtggccc gctcggtcgc cgcgtacgca
    18121 ggcccgggca tctacctcga cgtggcgatc tcgtgggttg cccggcgagc agtcacctcg
    18181 cccgtcgaga tgcgccgtga gggggatcgg ccgtccgggt actcgatgcg aacgttggtc
    18241 tcgcacttct ggcggctggt gctctccagc ggaactcgcc cgctgcgctg ggtcagtgtc
    18301 ctcggtgcca ccgcggtgct gctcgccccg gtcatcgcgc tggtgctgct catcggcaag
    18361 gcgctgggct cgtgggacgc acccgggtgg acgtccacga tgatcctgct catggtggcg
    18421 acaggcgtca tcctcttctc actcggggtg atcgccgagt acgtcgggat ggcggtcaac
    18481 atggcgatgg gcaagcccct ctacctgccg gttcgcgatc ggcgcgacgg gcccctcggc
    18541 cgcgacgcga gatgacgaca cgcccgacac agccgctcac gctggtcgtg ggggcgggcg
    18601 gtctcgtcgg tcggcacctc gtcacggccc atgggcgggc gcgccacgag gtgcgggtgg
    18661 ccgcggtgcc ctggggcgac gagggcgaga gcatctcggc tctcctcctt gcgctcgacg
    18721 acttcagcca ggcgcggcag ggccgcccgt ggtcggtcgc ctggtgcgct ggcgcaggag
    18781 tcgtcgcgac ggcggaggat gccctcgccg cggaggtgag ggtcttcgac cgtgtcatgg
    18841 gcgcgttggc tgcgtcggtg ggcccggacg aggacggcgt cgtcttcctc gcgtcatcag
    18901 ccggcggcgt ctacgccggg tccacaggtg cccctttcga cgagcgcacc gacgcggcac
    18961 ccctcgttcc ctacggccgg gcgaagctgg ccatggagga cacggttcag cgggtggcgt
    19021 ccgagaccgg cgtgcgggca gtcatcgggc gcctcgccaa tgtctacggg cccggccaga
    19081 ccctcggcaa accgcagggc ctgttgtcgc agctctgcct cgccgacgcc actgggcgcc
    19141 cactcccgct ctacgtgtcc atggacacga ttcgtgacta cctgtacgtg ggcgacgcgg
    19201 cgaccatgat cgttcggtgt ctcgagctgg tccgcgggga gccacgcggt gcggtggtga
    19261 cgaaggtgct gtcatcgggg aggccggtga ccgtcgggta cctggtctcc gaggcgcgcc
    19321 gggtgttcca ccggccgttg cggaccattg cggtgggggg cgccggaacg gggcaggtgc
    19381 tcgaccttcg gctggcctca cgagtgtggc ccgacgtcga cgccctcgcg tccacgccgt
    19441 tggccgctgg cctgtcgcgc acggccgccg atgtcagggc ccgcgtggtg agcggtggac
    19501 cgctcgatcc ctgacgtgac cccgccggat gcgagggtcg gtggccttgg ctcagccgac
    19561 ccgggtggcg gcgaggaaga ccgatgagcc gaagggcagg tcagcacgcg acaggatgtg
    19621 ccggtcgaag cgtgacaggg ccagcagcgc gtcgttgagc ggcttcggaa ccgtcgggac
    19681 ctcgacgatg tcgacggcgc cctgggccct cctccctcgc agcttccgcg cggcccgttc
    19741 ggccgcgaac atcgggaaca ccgaggcgaa cccgtgggtg acgcgctgaa cgctgaagcc
    19801 ggccgcttcc acggctgacc gtgcgcgctg gcgggtgtaa cgccggtggt ggccgttggc
    19861 gacgtcgaag tcgctccagg cccaggtgta cgcgggcacg gacatgacaa agcgcccacg
    19921 gggcttcagc acccggcgca cctcggccag cgcctggctc tcggggtcgc agtgctcgat
    19981 gacgtcgaac gccgagacca cctcgaaggt ctcgtccgcg aaggggagtg cgagcgcaga
    20041 gccacacacc ccgtcgcttc ccaggccgcg tgggtcgatg tcgagcgcca cggttcgtga
    20101 gacggcatca cgaagccagg aggcgctggg gccgtctgcg ctgccgaggt cgagcgtgag
    20161 gccagacccc tgcacgtgag atcgcagcgc ggtctcgaga aggtcggccc gtgcgacgta
    20221 ccaccagtag tcgggcacct cgatggaggg ggaccccccg tgtccgtggg ccgaccgtgc
    20281 gtggcttttc actgactcac cctatctacg ggcgcaccag tcctcacagc cgcactcggc
    20341 gcggggtgac cggctgcagc ccatcgttca cggcagggcc gaggccgcga gcgcctcacc
    20401 gggacgcacg agccgaaccg cgagctcgcc gtacgtggcc tcgatcgcgg cgggggtgcg
    20461 gcgcacgtcg ggggtgtacc agcgagccac gtcgatggcc atcgacagca gcgcgagtgc
    20521 ggtcgcgggc acgtcggtca ccgcgaactc cccggtctcg acaccgtcgc gcagcacacc
    20581 ggcgacgacc tggtcgatct ccttgcgcag cccgagcacg gcatcccggt gctctgatcg
    20641 caggtgacgg aactcgaact ggacgatccg accgagctcg tagtgctcgg cgtgccaccg
    20701 cgagaagccg ccgatgatgg cgcgcagcgc ctcggtgggg ttggcagccg cggcggccgc
    20761 ctcgacgagc aggtcacggg ctcgctcgtg accgcgccgg ctcagctcga acagcagcac
    20821 ctccttggag gcgaagtgca cgtagacccc ggcgggggac aggccggcgc gggatgcgat
    20881 gtcccgcgtc gtggtcgcgt ggaagccgcg ctgcgcgaag gcatcagcgg cggcgagcat
    20941 gatccgcacc gcggtgtcac tgacgtcgcc gacggcactc tggcgcccac ctgcgggcgc
    21001 gagggcatcg aggagggcgc tctcgctcag gggtgtggtg ctcggcacgg ttgacagcat
    21061 gccgtaccca cgcgaaacta agcaagcgct tagtcaacgt cgatccggat ctgtgatccg
    21121 cccggaggag ccagcatgcc ccgcaacatc tacggtcccg agcacgaaga cttccgagcc
    21181 tcggtgagcg agttcgtcga gcgcacgctc aagccgcgcg ccgagcagat gctcgaggtc
    21241 aagtcgatcg accgcgacat ctggaaggaa gccggcagcc aggggctctt cggcctggac
    21301 atccccgagg agttcggcgg catgggcgcc gaggactacc gcttcaacgc ggtctccgcc
    21361 gaggtgatca acggcttcaa cgccgccgtc ggctcgtgct tcggcatcca cagcgacgtc
    21421 tgccccccgt acatcgtcga cctcggcacc caggagcaga aggagcgttg gttgcccgcc
    21481 atggccgccg gcgagaagat ctgcgcgatc gcgatgaccg agcccggggg tgggtcggac
    21541 ctcgcagccc tgaagaccaa tgccgtgcgc gatggtgatg actggatcct caacggcagc
    21601 aagaccttca tcaccaacgg ctaccaggcc gacctggtca tcgtcgcgac ccgcaccgac
    21661 ccgaccaagg gcgccaaggg catcaccttg ttcatggtcg aggacggcat ggagggcttc
    21721 acccgcggcc gcaagctcga caaggtcggc caggaggagt ccgacaccgc cgagctgttc
    21781 ttccaggacg tgcgggtgcc cgacagcaac cgcatcggcg acgagggcat gggcttcatc
    21841 gcgatgatgc agcgcctgcc gcaggagcgc gtcggtgccg ccgccgccaa cgtcgcccac
    21901 gccaagcaga tcctcctgga gaccatcgag tacgccaagg agcgcaaggc cttcggccag
    21961 gcgatcggct ccttccagca caacaagttc aagctcgccg agatggtcac cgcgatcgag
    22021 gtcgccgagg cctacgtcga cgactgcatc gaggcgcacg cgaagggtga gctgaccgcg
    22081 gtcgacgccg ccaaggccaa gtggtggagc gcgcaggtgc agaacgacgt gctcgacgag
    22141 tgcgtccagc tctacggcgg ctacggcttc atgaacgagt accgcgtcgc ccgcgcctgg
    22201 cgcgacgccc gcgtgacgaa gatctgggcc ggctccaacg agatcatgaa ggagctcatc
    22261 gggcgcgacc tcggcctctg aggccgggcc acacgcccac cggatgccgt ccgctcaccg
    22321 tgagcgggcg gcatccctcg tcgtgaggac cccgcccacg atgagtgact gcgcggccag
    22381 gtaggtggcc atcacccaga agccctggcc ggggaggtcg aacccctcgg cgaaggcgtt
    22441 gagcgcgatc aacccgtcag agacgaggaa gagcgcgccg ccgatcgcgg tcggcaggtt
    22501 cacgccggtc gcgagcaccg ccatggtgcc caacagcgtc ccgtagacga gcaccggcac
    22561 gagcatccct gcggcaccac cgacgcacgc cagcacgagg gcgacgacca cggcgacgta
    22621 ccccaggagc acggggcgcc gcacgtgcag cacgctgcgg gaccggaagg gccagaacgc
    22681 catgacgtag gcgacctgcg cgagcaggaa gaagccgatc atgatgagga aggcggtgtc
    22741 accctccgcc agtttcggtg cgctgtcacc cagccacgac agccccaggg ccacgagggt
    22801 cagcgtgatc agacggctgc gcggggcctc ggtgttggac cacaacgccg cggccagcag
    22861 cggcatgagg agcacctggg tcgcgtcggc cacctcgccg gcaccggtca gctgcgcgcc
    22921 gaggtgcagc acggcgacgg tcacgaacag gccgacgact ccaccgacga ggggggtgcg
    22981 agcgcgcacg gcagcgtccg cccgggtcag tcgagcggcc cgccggcgac gtagatgacc
    23041 tgcccggaca cgaagccggc ctcctgcgac accaggaacg cgatcgtcgc cgcgatgtcc
    23101 tccggctggc cgacccgctg caccggcacc tgcgacgccg cgtgcgcgag gaactcctcg
    23161 aacggcaccc cgatgcgctc cgccgtcgcc gcggtcatgt cggtctggat gaaccccggc
    23221 gccaccgcgt tcgcggtcac cccgaaccga ccgagctcga tggcgagggt cttggtgaag
    23281 ccctggagcc ccgccttggc cgcggagtag ttggcctgcc cgcggttgcc ctgggccgat
    23341 gacgacgaca ggttgacgat ccggccgtac ttctgggccg tcatgtgggc ctggcacgcg
    23401 cgggtcatga ggaaggcgcc cttgaggtgc accgccatca cggcgtccca gtcgccctcg
    23461 gtcatcttga agatgaggtt gtcgcggatg atcccggcgt tgttgacgag caccaccggc
    23521 gggccgagct cgtcggccac gcgggccacg cccgactcga cctgctcggc gtcggtgacg
    23581 tcgacgccca ggcccagggc cgtgccgccc tcggcgcgga tggcgtcggc aacggcctcg
    23641 caggcagcct cgtcgaggtc gagcacggcc acggcaaaac cgtcccgggc cagccgggtg
    23701 gcggtggccg ccccgatgcc gcgagctgct ccggtgacga cggcgacacg ttggttctcc
    23761 atgccccgca ccctaccgag gggtacttcg gatgccggac gcggccgaac gttttcgcgc
    23821 tgtcggtccc gaccggtaag tttgaaagaa cccgcgggtg gctcgtgtgc ccccgtgagc
    23881 ccagcaccgt cgctggtttt gtgaccaccc acggaaggca accagtgcag atctggcccg
    23941 gtaccgcata cccccttggc gccacctatg acggcagcgg cgtgaacttc gccctgttca
    24001 gcgaggtcgc agagcgggtt gagctctgcc tcatcgacga cgagggcgtc gagacccgca
    24061 tcgacctgcc cgaggtcgac ggcttcgtcc accacgccta cctgcccggg gtctcacccg
    24121 gtcagctcta cgggttccgg gtccacggtc cgttcgaccc cgccaacggt caccgctgcg
    24181 acccgagcaa gctgctcctg gacccgtacg ccaaggccat cgagggtcag gtcaccaacg
    24241 accaggcgtt gttctcctac gacttcaccg acccgaccaa gcgcaacacc gaggacaacc
    24301 tcggcaagac gatgctctcg gtcgtcatca acccctactt cgactggggc cacgaccggc
    24361 cgccgcgcca cgagtaccac gacaccgtca tctacgaggc gcacgtcaag ggcctgacga
    24421 tgacccaccc ggaggtgccc gaggagatcc gcggcaccta cgcgggcatc gcccacccgg
    24481 cgatcatcga ccacctgagc accttgggca tcacggcgat cgagctgatg ccggtgcacc
    24541 agttcgtgca ggacacctcg ctccaggcca agggcctgac gaactactgg ggctacaaca
    24601 cgatcggctt cctcgccccg cacaacgggt acgcccgtgg acgccgtggc gagcaggtca
    24661 ccgagttcaa ggccatggtc aaggcgctgc acgccgccga catcgaggtc atcctcgacg
    24721 tcgtctacaa ccacaccgcc gagggcaacg agttcggccc gacgctgtgc ttccgcggca
    24781 tcgacaacgc ggcgtactac cgcctcgtcg acgacgccaa ggagcactac tacgacacga
    24841 ccggcaccgg caacagcctg ctgatgcgcc acccgcacgt gctccagctg atcatggact
    24901 cgctgcgcta ctgggtcacc gagatgcacg tcgacggctt ccgcttcgac ctcgccgcca
    24961 ccctggcccg ccagttccac gaggtcgaca agctctcggc gttcttcgac ctcatccagc
    25021 aggacccggt catcagccag gtcaagctca tcgccgagcc gtgggacgtc ggcgacggtg
    25081 gctaccaggt cggcaacttc cccccgctgt ggacggagtg gaacggcaag taccgcgaca
    25141 ccgtgcgcga cttctggcgc ggc
//
LOCUS       DAOSOC010000020        24710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_2033_length_24710_cov_9.186047, whole genome shotgun sequence.
ACCESSION   DAOSOC010000020 DAOSOC010000000
VERSION     DAOSOC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 24710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24710
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_24710_cov_9.186047"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2073)
                     /locus_tag="PL014_03065"
     CDS             complement(<1..2073)
                     /locus_tag="PL014_03065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOT56103.1"
                     /translation="MPTAESPARHSGFRPDIEGLRAVAILTVLAHHAGLPLHGGFIGV
                     DIFFVISGFLITGLLVTELWTTGTISWSRFVGRRIRRLLPAAVLVLVLTSLVSYLVVP
                     GLRRRDIGVDIAASAAYVVNWVFARREVDYLASDIRPSPVQHFWSLAVEEQFYVLWPL
                     LLIVLALVVRRPSRRVVLGALGVLVAVSIVWSIWFSDTSPRPAFFTTTTRVWELGVGA
                     MLAVALAGRPRPASPVRGARVLGWAALLTLVAVAVLLPDGIDWPGAWALLPTVPTAVL
                     LWLGWQSGGGHGAGRVLGLAPMVWIGGLSYSIYLWHWPFIVLGEWTADAVGTTLPAWG
                     KVALAVASIGPAWLSWRFVETPIHHGPWLRERPRALLASGLALSLVGVLAALPLLPLR
                     SPFTTTPPDGVVVVADLGAATVVPGRVVTDVDDPGWVTPDPLVAGEDRPAADVDRCQV
                     DHAATEPVACTFGDPDGARTIALVGDSKAMQWLPALEAVAAERGWRIITWGKSACAFA
                     AAPAAEAGVAYPECDAWNEAVVRSLDQEVPDVVVTSGVARSAWAGEATDRGLLVQGYA
                     DRWRWLTDRGVPVVVVGDSPLSPDDLDICAARNPRELGRCTFAAQPAVAGSGLPVQQD
                     AVALVVADPSPEARAGSAVELLDLTPWICPGGQCPVVIGHVAVHRAGDHVTATYASTL
                     APQLAAAVD"
     gene            complement(2060..2620)
                     /gene="coaD"
                     /locus_tag="PL014_03070"
     CDS             complement(2060..2620)
                     /gene="coaD"
                     /locus_tag="PL014_03070"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT56104.1"
                     /translation="MTDTVRRAVCPGSYDPVTHGHLDVIARAAAIHDEVVVAVLHNPA
                     KAGTFSVPERIAFIERGVAERLGPGAGAGAADKGTGTGTGVAGTVRVEAFAGTLLVDV
                     CRDLDASVIVKGLRGSTDFEYELPMALMNRHLTGIETVFIPGDPALAHVSSSLVKEVV
                     RFGGDVTGLVPDEVRDALVDRLRADG"
     gene            complement(2630..3250)
                     /locus_tag="PL014_03075"
     CDS             complement(2630..3250)
                     /locus_tag="PL014_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOT56105.1"
                     /translation="MTDIEIPRPTSGDVIELILDDHRLFEDLLRECRRSDADRAAARQ
                     ALAELLVAHAVAEEEKVYPDLRRARAIGAHEEEHSEEEHAEINEALLAFLEAKGTDTQ
                     KYDDALEELATAVNHHAGEEETDILNPAREDLSIDRRESLGVAWATRRNQLLEEGCAS
                     AEQVAALLAEAVDEGVLAPEEAREEADAVKDAAKAKAKEIEEAAKK"
     assembly_gap    3306..3405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3423..3992)
                     /gene="rsmD"
                     /locus_tag="PL014_03080"
     CDS             complement(3423..3992)
                     /gene="rsmD"
                     /locus_tag="PL014_03080"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOT56106.1"
                     /translation="MTRIIAGTLGGRTLRTPPGSGTRPTSDRVREALFSALDARGAVA
                     GARVLDLYAGSGALGLEAASRGAAAVLLVESDRRAADVIAGNVRELGLGQRVRVSRST
                     VDAALARAAGEPEQVDLVFIDPPYDVDEDALGAVLARLASGWLGPEALVVVERSKRSP
                     EPTWPDGLERDGKPRKYGETTIWLARPAT"
     gene            complement(3992..6199)
                     /locus_tag="PL014_03085"
     CDS             complement(3992..6199)
                     /locus_tag="PL014_03085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOT56107.1"
                     /translation="MATESSKLAPIIATAAKKFATARDIHTVGDLLAFWPRRYRTRES
                     DLGSVTPGEFLVGVAEVKSATTRSMKARKGTMLNVVITDGTHDIDITFFKAWGHEKVL
                     VPGARGIFAGVVGTYNNRLQLTHPGYTMLDDFDAGDRKDLIPIYRAVGSLHTWTVTES
                     VRRVLDVLDEVPDPIPESVRTTRRLVSRTQALRGIHTPNSWEEVEQARRRLRYEEAFV
                     LQVTLAQRRRTASGQVTRPRATRSGGILEAFDSRLPFKLTAGQREVGATIADEMAREV
                     PMHRLLQGEVGSGKTVVALRAMLAAVDAGGQAALLAPTEVLAGQHHRTITTMLGDLAD
                     GGMLGGDENATRVALLTGSQPTAVRRRTLLDVASGDAGIVIGTHALLQENVDFFDLAL
                     VVVDEQHRFGVEQRDALRGKGVVPPHVLVMTATPIPRTVAMTVFGDLETSTLRELPAG
                     RAPITTHVVPEERPGWMARTWERVAEEVRAGRQAYVVCPRIGDDGTGDGSIVGITDGA
                     DLRDEVVPDDDGTGSGQAAAAPPRPLKSVYAVHAALLEQPALAGLSIGVLHGRLSAED
                     KDAIMLRFQQGDLDVLVSTTVIEVGVDVANASVMVVMDADRFGVSQLHQLRGRIGRGG
                     HPGLCLLVTGTDAEPAMTRLSAVAATTDGFELARLDLSQRREGDILGAAQHGRRTQLE
                     FLHILDDEDVIVDAREDAFALVAADPDLTDHPELAEAVRARVDAEQAAYLERG"
     gene            complement(6217..7920)
                     /locus_tag="PL014_03090"
     CDS             complement(6217..7920)
                     /locus_tag="PL014_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOT56108.1"
                     /translation="MLDVLTAADARHWALLTRAALAARRTEIDALNVFPVPDGDTGTN
                     LYLTFDAAIDEVVAVHEGAGILGTASLVRECHTLKRAILLSARGNSGVIVSQIVGGIC
                     DVVIEADAEVVDAPLLAAAFARGAAAARACVAHPQEGTILTVADAGAAAAVDAANRGG
                     SLADVAQAAVDAARAALARTPDQLEALARAGVVDAGGAGCVLMLESFHRAITGRWTDD
                     EDSLLAAGPVLHRRDEWRAGTAVGTGPAAHEVLPGHDPLDREADHGGDGPAYEVMYLL
                     NATDAAAVETLTHTLDGLGDSLLVVGGPDLWNVHVHVDDPGAAVEAGIAAGRPERIRI
                     SHFATQSRTREHPSPIAVVACAAGPGIATLLREAGATVVPSAAGRRASAGQLLAAVRA
                     TGAPSVVILPGDRDTLMAAGVAAEAAMAEGIDAHVVPARTTVQTLAALAVLDPARAVH
                     ANVVAMTGAAVATRHGAVSVATKEALTWAGVCHPGDILGIVDGDVAFLGAEIEVAARE
                     VLDRLLSAGGELVTLVVGADAAPGLGERAAAALERDRPEIEVLVLDGGQPVYPLLIGV
                     E"
     gene            8064..8258
                     /gene="rpmB"
                     /locus_tag="PL014_03095"
     CDS             8064..8258
                     /gene="rpmB"
                     /locus_tag="PL014_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT56109.1"
                     /translation="MAANCDVCGKGPSFGHSISHSHRRTKRRWNPNIQRVKALVGDAG
                     VTPKRLNVCTSCLKAGKVKR"
     gene            complement(8349..9422)
                     /gene="thiL"
                     /locus_tag="PL014_03100"
     CDS             complement(8349..9422)
                     /gene="thiL"
                     /locus_tag="PL014_03100"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOT56110.1"
                     /translation="MRLRDTSEGELLARIFPIYAGSGHVDEGSVPVGPGDDAAVVSAP
                     GGSVVATTDSMVCGLDWRDDWSSAHDVGVKLAAQNLADIAAMGAEPTALLVSLAADPD
                     LEVAWAVDLARGIASLASRAGAPVVGGDLSGAPAGVVVVSVTALGDLRGRAPVLRSGA
                     RPGDVVAVCGSLGRSGGGLALLHRGEGARGAAVWDEPGCSDGDGSGPDAGSSRSAAVR
                     ALVTYHRAPVPPWQAGPRAAASGAHALIDVSDGLVTDLTRIARASGVGVDLDAAVLRE
                     RFAAGPLTVALGEHEAWHQVLTGGEEHSLVGAFADEVSLPQDPDHPWVVIGRVLAAGP
                     DGPRVTVDGVVPDHARGWDHFAR"
     gene            9568..9801
                     /locus_tag="PL014_03105"
     CDS             9568..9801
                     /locus_tag="PL014_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOT56111.1"
                     /translation="MVQAYILIQTDVGRAATVAASISEIEGVALAEDVTGPYDVIARV
                     EASTVDELGKLVIAKIQDVEGITRTLTCTVVHV"
     gene            9888..10253
                     /locus_tag="PL014_03110"
     CDS             9888..10253
                     /locus_tag="PL014_03110"
                     /inference="COORDINATES: protein motif:HMM:NF023453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 family protein"
                     /protein_id="HOT56112.1"
                     /translation="MPAGGSTPACELAGAAFPAEVSGMPRVDTAPASPAVAAWGDPAV
                     IARCGVGAAPPTDVECLEVDGVGWIPQELSDGIRFTSFGTDPALEVLVPGAYSPEGLL
                     LPAFADAVRALPGNGLACR"
     gene            complement(10377..11531)
                     /locus_tag="PL014_03115"
     CDS             complement(10377..11531)
                     /locus_tag="PL014_03115"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOT56113.1"
                     /translation="MSTDPTLPARSDDAGAPRRIRVALVFGGRSSEHAVSCATASSVL
                     EALDRDRYDVVPVGIAKDGHWVLAPDDPQSLRLAAGHTPEVASSAPSVIVPTSATDRT
                     FVVQEPGQPPRTLGEVDVVFPLLHGPFGEDGTIQGLLDLADVRYVGSGVTASAAMMDK
                     HVMKVLLASAGLPVGPYVVISDKEWQRDRAAAMDAVHGLSWPVFVKPARAGSSMGITK
                     VSQPEELEAAIEAAREHDPKVVVEAAVVGREIECGVLEGRGIDATRTSVVGECIVVQN
                     HDFYDFDAKYLSAADVRLVTPAEVPDAVSARIREMAAKAFDAAGCEGLARVDFFLTES
                     GDVLVNEVNTMPGFTPSSMYPRMWAATGLSYPELIDELIALALERRTGLR"
     assembly_gap    11800..11899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11900..12981
                     /locus_tag="PL014_03120"
     CDS             <11900..12981
                     /locus_tag="PL014_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOT56114.1"
                     /translation="HPDTDHLSPATRVVALGREAAAPGQQVGAPLVLSSTYHAGGEVM
                     YARSGNPTWSAFEQALGDLEGGDALVFASGMAAVSAALSLLPHGGTVVAPDAAYNGVV
                     ATLEAREAEGSATVRRVDVTDTAAVIDALDGADLLWLESPTNPLLEVADLPALLGAAR
                     ERGVLSVVDNTFATPLLQRPLEHGADVVVHSVTKYLAGHSDVLLGAVVTASTPTGHAL
                     HERLFTHRTLAGAIAGPMEVWLALRGLRTLHLRVERAQASARELATRLASHRAVTRVR
                     HPGWGGIVSIEVAGGADAAQRVCESTHLWVYSTSLGGVESQLERRRRIPSEPPQVPEN
                     LVRLSVGIEDVDDLWNDLSQALDQA"
     gene            complement(12987..13985)
                     /locus_tag="PL014_03125"
     CDS             complement(12987..13985)
                     /locus_tag="PL014_03125"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOT56115.1"
                     /translation="MTSAAVYGTGSWGTAYASVLADAGTSVTMWGRRQEVVDQINAGT
                     NEDYLPELRLPSTIRATTDPFEAAAGADIVVLAVPSQTLRANLEVWGSALPSSAAIVS
                     LMKGVELGTTRRMSEVISEVGGVEPERVVIVSGPNLAREIAVKQPAASVVASSSPAMA
                     ELVAAACAAPYFRPYTGSDVVGTEICGAVKNVIALAVGMAEGMGMGDNSKASIITRGL
                     AETMRLGMALGGEAPTFAGLAGVGDLIATCMSPLSRNHSFGVRLGQGLAVSEVIAVTK
                     QTAEGVKSCTSILELARHTGVDVPIIEQVDAMIQHDRSAQEVVSALLSRPRKAETA"
     gene            complement(13982..14740)
                     /locus_tag="PL014_03130"
     CDS             complement(13982..14740)
                     /locus_tag="PL014_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT56116.1"
                     /translation="MSIPTDHSRPRAYRFVVAVLRPLLMVFTKRRWSGQENFPTEGGF
                     VVVTNHYSHLDPFVFGHFLVDHGFSPRFLGKVEVFRIPVVGWILTRADQIRVHRESGR
                     AIHAFRSAVEAVRAGKVVAIYPEGTLTRDPDMWPMRGKTGAARVALETGCPVVPVVTW
                     GAHEILGPYRHWPHLLPRKTMVVHAGPPVELDDLRGLPLTAEVLQDATDRIMAALTND
                     LEGVRGERAPGIRFDPRAAGVTPIGKPRPLKEAS"
     gene            14831..15694
                     /gene="cofC"
                     /locus_tag="PL014_03135"
     CDS             14831..15694
                     /gene="cofC"
                     /locus_tag="PL014_03135"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HOT56117.1"
                     /translation="MPDLVPQWAAVVPVKGGPLAKSRLALPEPARRDLANAFAHDTVS
                     ALLDAIEGMPVLVVTSDPTVSSWVTPAGARLVPDPGLGLDAAVAAGCRVAAAAGATRV
                     AVVLGDHPALRAAEVRVALEATGRHPAAVVPDADGLGTAMLTLTVPRGESMAGVRTAF
                     GAGSAAAHEALGHVRLDLDLPGLRVDVDDARSLAEATRLGLGPHSARALARATVHGVQ
                     ATIHRIADDGSGSALLDDGVEVDLPPDAAQRSGLRHLRVGQRVSIELDESGAAATRVW
                     ITGIGPGEDIH"
     gene            complement(15732..17048)
                     /gene="murA"
                     /locus_tag="PL014_03140"
     CDS             complement(15732..17048)
                     /gene="murA"
                     /locus_tag="PL014_03140"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT56118.1"
                     /translation="MTSSLVVDGGSPLVGDIEVRGAKNLVSKAMVAVMLAEEGCRLRG
                     VPEISDVQIVSGLLELHGVVIDSTDRDGELVLDPTNVEQAHIADIDAHAGSSRVPVLL
                     CGPLLHRLGEAFIPDLGGCRIGERPINYHLDVLRTFGAVVEKRQDGLRLTAPNGLHAA
                     DIHLPYPSVGATEQVLLTAVRAIGRTTLRGAAIEPEIMDLIAILQKMGAIISVQTDRV
                     ILIEGVDRLGGFDHLAIPDRIEAGSWACAALATRGDIYVRGAQQLPMMPFLNVFRRIG
                     GKFEIDDDGIRFWHPGGTLNSIAIETDVHPGFMTDWQQPLVVALTQTTGLSIVHETVY
                     ENRLGFTDALVEAGAHIQTFRECLGGSPCRFGRANYQHSAVISGPTPLQAAEITVPDL
                     RGGFSYVIAALAAEGRSTVHGIDLIHRGYERFSEKLDNLGAVYKLP"
     gene            complement(17114..17467)
                     /locus_tag="PL014_03145"
     CDS             complement(17114..17467)
                     /locus_tag="PL014_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT56119.1"
                     /translation="MNKADLVKELESTFGSRRAAAEALDAVLDTIIREVARGGKVSIT
                     GFGTFDRVERAPRTGRNPHTGTSVPIPEVKAPRFRAGTAFKSYVKEPHTLPEDGTATG
                     RAPAGTAAAKKSASA"
     gene            complement(17682..18272)
                     /gene="leuD"
                     /locus_tag="PL014_03150"
     CDS             complement(17682..18272)
                     /gene="leuD"
                     /locus_tag="PL014_03150"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOT56120.1"
                     /translation="MEAFTTHTGVGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFA
                     AWRGDETFVLNDPSYSAGSVLVAGPDFGTGSSREHAVWALMNYGFRVVISSRFADIFR
                     GNSGKQGLLTAQCSQDDVELLWKLLENHPGTSVTVDLVSRTITAGDLVARFEVDDYTR
                     WRLLEGHDDISLTLRHEGDISTFEGSRPSWKPSVTA"
     gene            complement(18272..19687)
                     /gene="leuC"
                     /locus_tag="PL014_03155"
     CDS             complement(18272..19687)
                     /gene="leuC"
                     /locus_tag="PL014_03155"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOT56121.1"
                     /translation="MAGTLAEKVWESHVVHRGEGEPDLLYIDLHLLHEVTSPQAFDGL
                     RLAGRPVRRPDLTLATEDHNVPTTPGPITDPVSRTQVETLRRNCEEFGVTLYPMGDAE
                     QGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPLKP
                     FKTMAITVEGDLADGVTAKDIVLAVIARIGTGGGQGYVLEYRGSAIRALSMEARMTVC
                     NMSIEAGARAGMIAPDETTFEYLRGRDHAPTGADWDAAVAAWSELRSDDDAVFDDEVV
                     LDAAALTPFVTWGTNPGQGAPLGSAVPDPELMPDENDKANARRALEYMGLEAGTPLRE
                     VHVDTVFLGSCTNGRIEDLRAAASVLQGRQVADGVRMLVVPGSARVRLQAESEGLHEV
                     FTAAGAEWRLPGCSMCLGMNPDQLAPGERSASTSNRNFEGRQGKGGRTHLVSPLVAAA
                     TAVRGTLSSPADLEPVTAGSN"
     gene            19842..20585
                     /locus_tag="PL014_03160"
     CDS             19842..20585
                     /locus_tag="PL014_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT56122.1"
                     /translation="MDNTSGVGVLDKAAIVLSALEAGPSTLAQLVTATGLARPTAHRL
                     AVALEHHRLVTRDLQGRFILGPRLGELAAAAGEDRLLAAAGPVLGALRDHTNESAQLF
                     RRQGEHRVCVSAAERPVGLRDSIPVGAALSMRAGSAAQVLLAWEEPDRLHRGLQGAAF
                     SATSLSGVRRRGWAQSVGEREPGVASVSAPVRSPSGRVIAAVSISGPIERLSRQPGRL
                     HAATVIAGANKLTEVLARHAAQQDAQNNA"
     assembly_gap    20589..20688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20689..21060
                     /locus_tag="PL014_03165"
     CDS             <20689..21060
                     /locus_tag="PL014_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT56123.1"
                     /translation="DVRRARRLPVSLTTAQRIARDRTRLLGFPRPGRRAIVVGGGAMA
                     ARRAAALTRATTPVTVFAPALCDDVFDLLAERLVTWENRWPVEADLADAWLVHAATGD
                     PELDARICLWASTVREGGLSA"
     gene            complement(21050..21505)
                     /locus_tag="PL014_03170"
     CDS             complement(21050..21505)
                     /locus_tag="PL014_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HOT56124.1"
                     /translation="MVRQVGDVEAVGPVTQRLLPHGPDHSHGWGRRPLRVAFPPSRNR
                     SWCRVCRARTPSASSGAGPSTSASGTTSVIRRGATGLGAAPGPPAWLAFHRAARQDPS
                     AVTIWHETYAVSAAESLYAGPAPFGLADVGGTVPVGRRGETARERIVRR"
     gene            21443..21613
                     /locus_tag="PL014_03175"
     CDS             21443..21613
                     /locus_tag="PL014_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56125.1"
                     /translation="MRQQALRDGAHCLDVTHLPDHVPSGAALLAIALGEEARGIRGLD
                     VLRRSVDRAPPA"
     gene            21813..22022
                     /locus_tag="PL014_03180"
     CDS             21813..22022
                     /locus_tag="PL014_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOT56126.1"
                     /translation="MPTLPADDVAAAHRASVERTLHEVRLALRMYAEGRYGTCIRCAD
                     PISVVRLEARPWSTRCIRCADLVGW"
     gene            22019..23290
                     /locus_tag="PL014_03185"
     CDS             22019..23290
                     /locus_tag="PL014_03185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT56127.1"
                     /translation="MTPGEGGRPRQVVVVGAGMVGLSTAWFLQDHGIDVTVIERGDVA
                     AGASWGNAGWLTPGLTAPLPDPAVLGQGLRALLDPSSPVVVPRIPSPRLLRFLTGFAR
                     HSTTRRWRAGMSAYLQVNAMALGAFDRLAEGGVSEPTHEADPFLACYRTPEDRRFLLE
                     ELEQMAGAGQDVRFDILTGEEARRCEPALSPTVGAGIRIHGQRYIHPPRFMDALAASF
                     RARGGTILTGRTVTGLQDRGESVEVADDTGITHRVDAVVVASGAELGDLTRAFGVRHL
                     VQAGRGYSFTVRARQLPSGPVYFPAQRLACTPLRSPTGDRLRVAGTMEFRSPSAPLDR
                     ERIDVMAAALAPLMDGLDLHERQEEWVGSRPCTTDGLPLVGGTRSRRVFVAGGHGMWG
                     IVLGPVTGQLLASLVATGQLHPALTPFDPTR"
     gene            complement(23351..23423)
                     /locus_tag="PL014_03190"
     tRNA            complement(23351..23423)
                     /locus_tag="PL014_03190"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23387..23389),aa:Glu,seq:ctc)
     gene            complement(23529..23603)
                     /locus_tag="PL014_03195"
     tRNA            complement(23529..23603)
                     /locus_tag="PL014_03195"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23568..23570),aa:Gln,seq:ctg)
     gene            complement(23725..>24710)
                     /locus_tag="PL014_03200"
     CDS             complement(23725..>24710)
                     /locus_tag="PL014_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOT56128.1"
                     /translation="SAPRPDDQVIFKADGFPTYHLAVVVDDHEMGITHVVRGEEWISS
                     TPKHILLYQWLGLEPPKFAHMPLLRNPDSSKISKRKNPAARLTWFREQGYLPEALVNF
                     LALLAYPPAKGPEGEDVEVFTFEDFSARFSWSDVNPVGPIFDLKKLDWLNGMHIRALE
                     LDDFTSRLLPYLEADGILSGNPSLGELGRLREVAALIQTRIAHLTEAGALVAPFFAPD
                     DMVDVADDARAQLGEDAVRVLDAASTALQALPDEHSGVLASESTWTAPAIEAALRAAV
                     IDGLGLKPKVAFTPLRTAVSGRRISPPLFESMEILGKTATLTRLDALRSTL"
BASE COUNT         3473 a         8875 c         8722 g         3340 t
ORIGIN      
        1 gtcgacggcc gcggccagct ggggggccag cgtcgaggcg taggtggcgg tgacgtggtc
       61 cccggcccgg tgcaccgcga cgtggccgat gacgaccggg cactggccac cggggcagat
      121 ccacggggtg aggtcgagca attcgacggc ggagccggcc cgcgcctcgg gggacggatc
      181 agcaacgacg agcgccacgg catcctgctg caccggcaac ccgctgcccg cgaccgccgg
      241 ctgcgccgcg aaggtgcagc gcccgagctc tcgcgggttg cgggccgcac agatgtcgag
      301 gtcgtccggc gagagcgggc tgtccccgac gacgacgacg ggcacacccc gatccgtgag
      361 ccaccgccag cggtcggcgt acccctgcac gagcagtcca cggtcggtgg cctctcccgc
      421 ccacgcactg cgagccaccc ccgacgtcac cacgacgtcc ggaacctcct ggtcgagcga
      481 gcgcaccacg gcctcgttcc aggcgtcgca ctcggggtag gcaacccccg cctcggccgc
      541 cggcgcagcg gcgaacgcgc acgctgactt gccccacgtg atgatccgcc acccgcgctc
      601 ggccgcgacc gcctcgagag caggcagcca ctgcatcgcc ttggagtcac cgacgagggc
      661 gatcgtcctc gccccgtcgg ggtcaccgaa cgtgcacgcc accggctcgg tggcggcatg
      721 gtcgacctgg cagcgatcga cgtcggcggc cggccggtcc tcaccggcca cgagggggtc
      781 aggcgtcacc cagccggggt cgtcgacatc cgtgacaacc cgcccgggga ccacggtggc
      841 cgcaccgagg tcagccacga cgaccactcc gtccggtggc gtcgtggtga acggtgagcg
      901 cagcggcagc agcggcaggg cagcgagcac gcccaccagc gacagtgcca gcccgctcgc
      961 gagcagggcc cgcgggcgct cgcgcagcca cggaccgtgg tggatgggcg tctcgacgaa
     1021 gcgccacgac agccaggccg ggccgatcga cgcgacggcg agcgcgacct ttccccaggc
     1081 cgggagagtg gtgcccacgg catccgcggt ccattccccg agcacgatga acggccagtg
     1141 ccacaggtag atcgagtacg acaacccgcc gatccacacc atcggcgcca acccgagcac
     1201 ccggcccgca ccgtggccgc cgcctgactg ccagccgagc cacagcagca ccgcggtcgg
     1261 caccgtcggc agcagcgccc acgcccccgg ccagtcgatg ccgtcgggca gcaacaccgc
     1321 gaccgccacc agggtgagca gggccgccca gccgagcacc ctcgcgccac gaactgggga
     1381 tgccggtcgc ggcctgccag cgagcgccac cgcgagcatc gcccccaccc cgagttccca
     1441 cacccgggtg gtcgtcgtga agaacgccgg ccggggggag gtgtccgaga accagatcga
     1501 ccagacgatg gagaccgcga cgagcacgcc gagtgcgccg agcacgaccc ggcggctcgg
     1561 gcgccgcacg accagggcca gcacgatgag cagcagcggc cacaggacgt agaactgctc
     1621 ctcgacggcc agcgaccaga agtgctgcac gggtgagggg cggatgtcgg aggcgaggta
     1681 gtcgacctct cgccgggcga acacccagtt gacgacgtag gccgcggatg ccgcgatgtc
     1741 gaccccgatg tcgcgccgtc gcaacccggg caccacgagg tacgacacga gcgaggtgag
     1801 caccaggacg agcaccgcgg cgggcagcag gcgccggatc cgtcgaccga cgaaccgcga
     1861 ccaggagatc gtcccggtgg tccacagctc ggtgacgagc aacccggtga tgaggaagcc
     1921 ggagatgacg aagaagatgt cgacgccgat gaacccgcca tgcagcggca ggcccgcgtg
     1981 atgggccagg acggtgagga tggcgaccgc gcgcaggccc tcgatgtccg ggcggaaacc
     2041 cgagtggcgc gcgggcgact cagccgtcgg cacgcaggcg gtcgacgagg gcgtcgcgca
     2101 cctcgtccgg caccaggccg gtcacgtcgc cgccgaagcg cacgacctcc ttgacgagcg
     2161 agctcgacac gtgcgccagc gccgggtcgc ccgggatgaa gaccgtctcg atgccggtga
     2221 ggtggcggtt catcagcgcc atcgggagct cgtactcgaa gtcggtggag ccgcgcagcc
     2281 ccttgacgat caccgaggcg tcgaggtcgc ggcagacgtc gacgagcaac gtgccggcga
     2341 aggcctcgac ccgcaccgtg cccgcgacgc cggtgccggt accggtgccc ttgtcggcag
     2401 caccggcacc agcgccagga ccgagacgct cggcgacacc ccgctcgatg aacgcgatcc
     2461 gctccggcac gctgaacgtc cccgccttgg ccgggttgtg cagcacggcc acgacgacct
     2521 cgtcgtggat cgccgcagcc cgggcgatga cgtcgaggtg accgtgggtc acggggtcgt
     2581 aggagcctgg gcacacggct cggcgcacgg tgtcggtcac acccccacgc tacttcttgg
     2641 cggcctcctc gatctccttc gccttcgcct tcgctgcgtc cttgacggcg tcggcctcct
     2701 cgcgagcctc ctccggggcg agcacgccct catccaccgc ctctgcgagc agggccgcca
     2761 cctgttcggc ggaggcgcag ccctcctcga gcaactggtt gcggcgcgtc gcccaggcga
     2821 ccccgagtga ctcgcggcgg tcgatggaca ggtcctcgcg ggccgggttg aggatgtcgg
     2881 tctcctcctc ccccgcgtgg tggttcaccg cggtggcgag ctcttcgagg gcgtcgtcgt
     2941 acttctgggt gtcggtgccc ttggcctcca ggaacgcgag cagggcctcg ttgatctcgg
     3001 cgtgctcctc ctcgctgtgc tcctcctcgt gggcgccgat ggcgcgggcg cggcgcaggt
     3061 cggggtagac cttctcctcc tcggccacgg cgtgcgcgac gagcagttca gccagcgcct
     3121 ggcgggcggc ggcgcggtcg gcgtcgctgc ggcggcactc gcgcagcagg tcctcgaaga
     3181 ggcggtggtc gtcgaggatc agctcgatga cgtcgccgga agtgggtcgg gggatctcga
     3241 tgtctgtcat tgacccatcg tcaccaattc gccgaccgaa atgtgcccga gcaaccgact
     3301 tcgtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccgg atgccgggtg
     3421 ggtcaggttg cggggcgggc cagccagatc gtggtctcgc cgtactttcg gggtttgccg
     3481 tcccgttcga gcccgtcggg ccaggtcggc tccgggctgc gcttcgaccg ctcgacgacc
     3541 acgagcgcct cggggccgag ccaccctgac gcgagccggg ccagcacggc gcccagggcg
     3601 tcctcgtcga cgtcgtaggg cgggtcgatg aacaccaggt cgacctgctc cggctccccc
     3661 gccgcgcggg ccaaggcagc gtcgaccgtc gagcgcgaca cccgcacccg ctgaccaaga
     3721 cccagctcgc gcacgttgcc cgcgatcacg tcggccgcgc gccggtcgga ctccacgagc
     3781 agcaccgccg ccgctcccct gctggcggcc tcgagcccca gggcccctga cccggcatag
     3841 aggtcgagca cccgtgcacc ggccacggcg ccgcgggcgt cgagcgcgct gaacagggcc
     3901 tcgcgaaccc ggtcactcgt cggccgggtg cccgatccgg gtggagtgcg cagcgtgcga
     3961 cccccgaggg ttccggcgat gatgcgggtc atcagccacg ctccaggtag gcggcctgct
     4021 cggcgtcgac ccgggcacga acggcctcgg cgagctcggg gtggtcggtg aggtcggggt
     4081 cggcggcgac gagggcgaag gcgtcctcac gggcatcgac gatgacgtcc tcgtcgtcca
     4141 ggatgtgcag gaactccagc tgcgtgcgcc gcccgtgctg cgccgcgccg aggatgtccc
     4201 cctcgcgccg ctgtgacagg tcgagccggg ccagctcgaa gccgtcggtg gtggccgcca
     4261 cggccgacag gcgcgtcatc gccggctccg cgtcggtgcc ggtgacgagc aggcacagcc
     4321 cggggtgccc accgcgaccg atccgccccc gcagctggtg cagctgcgag acaccgaacc
     4381 ggtcggcatc catgacgacc atgacgctgg cgttggcgac gtcgaccccc acctcgatga
     4441 cggtggtcga gacgagcacg tcgagatcgc cctgctggaa ccgcagcatg atggcgtcct
     4501 tgtcctcggc actgagccgg ccgtgcagca cgccgatcga caacccggcc agggcgggct
     4561 gctcgagcag cgccgcgtgc acggcgtaga cgctcttcaa tggccgcggc ggtgccgcag
     4621 ccgcctggcc ggaaccagtg ccgtcatcgt cgggcacgac ctcgtcgcgc aggtcggccc
     4681 cgtcggtgat gcccacgatg gagccgtcac cggtgccgtc atcgccgatc cgcgggcaga
     4741 cgacgtaggc ctggcgaccg gcccggacct cctcggccac ccgctcccag gtgcgggcca
     4801 tccaccccgg ccgctcctcc ggcacgacgt gggtggtgat cggcgcccgc ccggcaggca
     4861 gttctcgcag ggtggaggtc tcgaggtcgc cgaacaccgt catcgccacg gtgcgcggga
     4921 tcggcgtcgc cgtcatcacc aggacgtgcg gcggcacgac tcccttgccg cgcagggcgt
     4981 cgcgctgctc gaccccgaac cggtgctgct cgtcgacgac gacgagcgcg aggtcgaaga
     5041 agtcgacgtt ctcctggagc agggcgtgcg tgccgatcac gattccggcg tcaccactgg
     5101 cgacgtcgag cagggtccgg cggcgcacgg ccgtgggttg gctacccgtc agcagggcca
     5161 cccgggtcgc gttctcgtca ccaccgagca tcccgccgtc ggcgaggtca ccgagcatcg
     5221 tggtgatcgt gcggtggtgt tgcccggcga gcacctcggt gggcgccaac agcgccgcct
     5281 gccccccggc atccaccgcg gccagcatcg cccgcagggc gacgacggtc ttgcccgagc
     5341 ccacctcacc ctggagcaga cggtgcatcg gcacctcgcg ggccatctcg tcggcgatcg
     5401 tcgcgccgac ctcgcgctgc cccgcggtca gcttgaacgg cagccgggag tcgaaggcct
     5461 cgaggatgcc ccccgacctc gtcgcgcggg gccgggtcac ctgcccggat gccgtgcggc
     5521 gccgctgcgc caacgtcacc tggaggacga acgcctcctc gtaccgcagc cgccgccggg
     5581 cctgctcgac ctcctcccac gagttcgggg tgtggatgcc gcgcagcgcc tgggtgcgtg
     5641 agacgagtcg gcgggtggtc cgcacgctct cggggatcgg gtcgggcacc tcgtcgagga
     5701 cgtcgagcac ccggcgcacg ctctcggtga cggtccacgt gtgcagcgac ccgaccgcgc
     5761 ggtagatggg gatgaggtcc ttgcgatcgc cggcgtcgaa gtcgtcgagc atcgtgtagc
     5821 ccgggtgggt cagctggagg cggttgttgt aggtgccgac caccccggcg aagatgccgc
     5881 gtgcgccggg cacgagcacc ttctcgtgac cccaggcctt gaagaaggtg atgtcgatgt
     5941 cgtgcgtgcc gtcggtgatg acgacgttga gcatggtgcc cttgcgcgcc ttcatcgacc
     6001 gcgtggtcgc gctcttgacc tcggcaacac cgacgaggaa ctcgccgggc gtcaccgacc
     6061 ccaggtcgct ctcgcgggtg cggtagcggc gcggccagaa cgccagcagg tcgcccacgg
     6121 tgtggatgtc gcgggccgtg gcgaacttct tggccgcggt ggcgatgatc ggtgccagct
     6181 tgctcgactc cgtcgccacg tcaggtcacc gccaactcac tccaccccga tgagcagcgg
     6241 atagacgggt tgccccccgt cgaggacgag gacctcgatc tcggggcgat cgcgttccag
     6301 ggcggccgcg gcccgttcac cgaggccggg cgcggcgtcg gccccgacga cgagggtcac
     6361 gagctctcca ccggcggaca gcaaccggtc gaggacctca cgggccgcga cctcgatctc
     6421 ggcgccgagg aaggcgacgt cgccgtcgac gatgccgagg atgtcgccgg ggtggcagac
     6481 gcccgcccag gtcagggcct ccttggtcgc gacggacacg gcaccgtggc gggtggcgac
     6541 ggccgcaccg gtcatcgcga cgacgttggc gtgcacggca cgcgcagggt ccaggaccgc
     6601 gagcgcggcc agggtctgca cggtcgtgcg ggccggcacg acatgggcgt cgatgccttc
     6661 ggccatggcg gcctcggccg cgaccccggc cgccatgagc gtgtcgcggt cgccgggcag
     6721 gatcaccacc gacggcgcgc ccgtggcccg caccgccgcg agcagctggc cggccgatgc
     6781 gcgccggccc gcagccgacg ggacgaccgt ggccccggcc tcgcgcagca gggtggcgat
     6841 gcccgggccg gcggcacacg ccaccacagc gatgggtgag gggtgttcgc gggtgcgtga
     6901 ctgggtggcg aagtgggaga tgcggatccg ctcggggcgt ccggcggcga ttccggcctc
     6961 gaccgcagcc ccggggtcgt cgacgtgcac gtgcacgttc cagaggtcag gccccccgac
     7021 gacgaggagg gagtcgccga gcccgtcgag ggtgtgggtc agggtctcca cagccgccgc
     7081 gtccgtggcg ttcagcaggt acatgacctc gtatgccggg ccgtccccac cgtggtcggc
     7141 ctcgcggtcg agcgggtcgt gaccgggcag cacctcgtgg gccgcggggc cggtgccgac
     7201 cgcggtgccg gcacgccact cgtcgcggcg gtgcaggacc ggaccagccg cgagcaggct
     7261 gtcctcgtcg tcggtccagc gcccggtgat ggcgcgatgg aacgactcga gcatcaggac
     7321 acacccggcc ccgccggcgt cgacgacccc ggcccgggcg agggcctcga gctggtcggg
     7381 ggtgcgcgcc agggctgcac gggcggcgtc gacggcggcc tgagcgacgt cggccagcga
     7441 cccaccgcgg ttggcggcgt cgacagcggc ggcggccccg gcatccgcga cggtgaggat
     7501 cgtgccctcc tgcgggtgcg cgacgcacgc gcgggccgcg gcggccccgc gggcgaaggc
     7561 tgccgcgagc agcggggcgt cgaccacctc ggcatcggcc tcgatgacga cgtcgcagat
     7621 gcccccgacg atctggctga cgatgacacc ggagttgccg cgcgccgaga gcaggatcgc
     7681 ccgcttgagc gtgtggcact cgcgcaccag gctggcggtg ccgaggatgc cggcgccctc
     7741 gtggacggcg accacctcgt cgatggccgc gtcgaaggtg aggtagaggt tggtcccggt
     7801 gtcgccgtcg ggcacgggga agacgttgag ggcgtcgatc tcggtacgtc gggccgcgag
     7861 cgccgcacga gtcaacagcg cccagtggcg ggcgtcggca gcagtgagga cgtcgagcac
     7921 gccctcaccc taaccgtcac ggccgacgcc cgcggggagg cgttttggtt gctcgggcgt
     7981 ggctcggcta ctcttgtccg gttgcgcgcc tgctcgtgcg ggccgccgaa gaccatcaat
     8041 cacaccatct caggagtaca cccgtggctg ccaactgcga cgtctgcggc aagggaccct
     8101 ctttcgggca cagcatctcg cactcgcacc gccgcaccaa gcgtcgctgg aaccccaaca
     8161 tccagcgcgt caaggcgctc gtcggcgacg ccggtgtgac cccgaagcgg ctcaacgtct
     8221 gcacctcctg cctcaaggcg ggcaaggtca agcgctgagg tcccctcacg caccatgaac
     8281 cgggcggtcc ccttgggggc cgcccgtttt cgttgccctc gggaggctgg gcgagatcgg
     8341 gatgccgttc agcgggcgaa gtggtcccat cccctcgcgt ggtcgggcac cacgccgtcg
     8401 acggtgaccc ggggcccgtc cgggccggcc gcgagcaccc gcccgatgac gacccacggg
     8461 tggtccgggt cctgcggcag cgagacctcg tcggcgaagg ccccgacgag ggagtgctcc
     8521 tcgccgccgg tgagcacctg gtgccaggcc tcgtgctcgc cgagggcaac cgtgagcggc
     8581 cctgcggcga accgctcgcg cagcacggct gcgtcgaggt cgacgccgac cccgctggcc
     8641 cgggcgatcc gggtcaggtc ggtgaccagc ccgtccgaga cgtcgatgag agcgtgcgcc
     8701 ccggaggccg cggcgcgcgg ccccgcctgc cacggtggca ccggcgcccg gtggtacgtc
     8761 accaacgcgc gcacggccgc ggaccgcgac gacccggcat ccggcccgct cccgtcaccg
     8821 tcgctgcacc ccggctcgtc ccagacggcc gcgccgcgcg ctccctcacc ccggtggagc
     8881 agcgccagcc cgcccccgga ccggccgagc gacccgcaca cggcgacgac gtccccgggc
     8941 cgcgcgcccg agcgcagcac gggcgcgcgc ccgcgaaggt cacccaacgc cgtcaccgac
     9001 acgacgacga ccccggccgg tgcccccgag aggtcgccgc ccaccaccgg cgcccccgcc
     9061 cgggacgcca gcgaggcgat gccgcgggcg aggtcaaccg cccacgcgac ctccaggtcg
     9121 gggtcggcag cgagcgagac gagcagggcc gtcggctcgg cccccatcgc agcgatgtcg
     9181 gcgaggttct gggccgcgag cttcaccccc acgtcgtgcg cgctcgacca gtcgtcacgc
     9241 cagtccaggc cgcacaccat cgagtcggtc gtcgcgacga cgctgccgcc gggggcgctg
     9301 accacggccg cgtcgtcgcc cgggccgacc ggcaccgagc cctcgtcgac gtgcccgctc
     9361 ccggcataga tcgggaagat ccgagcgagg agttcaccct cgctggtgtc gcgcaaccgc
     9421 accgtgacct accctcccgg agacgtgggg acccctcgca cggtagcgtg cgggcagcgc
     9481 cggtcacgac gggcgccccg gccgcaccgg gccaaccggc ccgacggctc gacctgcaca
     9541 gacccgacac gaagaggtgg cactccagtg gtacaggcct acatcctgat ccagaccgac
     9601 gtcggccgcg ccgcgaccgt cgctgcgtcg atctccgaga tcgagggcgt tgccctcgca
     9661 gaggacgtca ccggccccta cgacgtgatc gcccgcgtcg aggcctccac ggtcgacgag
     9721 ctcggcaagc tcgtcatcgc caagatccag gacgtcgagg gcatcacccg aaccctcacc
     9781 tgcaccgtgg tgcacgtctg attcggtacc gactccggac gacggcggct gcgctcggcg
     9841 cggccgccgt cgtgctgtcc ggatgttcct cggcggtcga ggtctcggtg ccggcagggg
     9901 gttcgacccc cgcctgcgag ctggctgggg cggccttccc cgcggaggtc tccgggatgc
     9961 cgcgcgttga caccgcgccc gcgtcgcctg ccgtcgcggc ctggggcgac cccgcggtca
    10021 tcgcccgctg cggcgtcggc gcggcaccgc cgaccgatgt cgagtgcctg gaggtggacg
    10081 gggtggggtg gatcccgcag gagctgagcg acggcatccg gttcacctcg ttcggcaccg
    10141 accccgcgct cgaggtgctg gtaccgggcg cgtactcgcc ggaggggttg ctgctgcccg
    10201 ccttcgcgga tgccgtgcgc gcgcttcccg gcaacggcct cgcctgccgc tgacccgcgg
    10261 cccgacggtg gcaccaagcc acgccaagcg gacacccagg ggcgcagccc gacacgggtt
    10321 ggcggtgcgg tgcccagcag agccggcgtc agcgacgccg tgcgacgcaa gggcggtcag
    10381 cgcagaccgg tgcggcgttc cagcgccagg gcgatgagct cgtcgatgag ctctgggtag
    10441 ctcagcccgg tggccgccca catccgcggg tacatggagc tcggcgtgaa cccgggcatc
    10501 gtgttcacct cgttgacgag cacgtcgccg ctctcggtga ggaagaagtc gacccgggcc
    10561 agaccctcgc agccagccgc gtcaaacgcc ttggccgcca tctcgcgaat ccgggccgac
    10621 acggcatccg gcacctcggc cggggtcacg aggcgaacgt ccgcggccga gaggtacttc
    10681 gcgtcgaagt cgtagaagtc gtggttctgc acgacgatgc actcccccac gacactcgtg
    10741 cgggtcgcat cgatgccacg accctcgagc accccgcact cgatctcgcg cccgacgacc
    10801 gccgcctcga cgacgacctt ggggtcgtgc tcgcgggccg cctcgatcgc cgcctcgagt
    10861 tcctcgggct gcgacacctt ggtgatgccc atgctcgacc cggcccgcgc gggcttgacg
    10921 aacaccggcc acgagagccc gtgcacggca tccatcgccg cggcgcggtc gcgctgccac
    10981 tccttgtcgc tgatgacgac gtatgggccg accggcagcc ccgccgaggc cagcagcacc
    11041 ttcatgacgt gcttgtccat catcgccgcg gatgccgtga cccccgaccc gacgtacctc
    11101 acgtccgcga ggtcgagcag accctggatc gtgccgtcct caccgaacgg accgtgcagc
    11161 agtgggaaga cgacgtcgac ctcgcccagt gtgcgcggcg gctgcccggg ctcctggacg
    11221 acgaaggtgc ggtcggtggc actggtcggc acgatgacgc tgggggcgct gctggcgacc
    11281 tcgggggtgt gccccgccgc gaggcgcaac gactgcgggt cgtccggggc gagcacccag
    11341 tgcccgtcct tggcgatgcc gacgggcacg acgtcgtagc ggtcgcggtc gagcgcctcc
    11401 aacacgctgg atgccgtcgc gcacgagacc gcgtgctccg aggagcggcc accgaacacg
    11461 agggcgacgc ggatccggcg gggcgcaccc gcatcatcgg aacgggcagg cagggtcggg
    11521 tcggtgctca tcgccgtcga ctctatcggc tgaccgggcc cgatcccggg cgcaggcccg
    11581 atgcaggtcc gtgctcccga gtgctgggcc ttgctccgcc ggaagatcct tgctccggtt
    11641 gtgcgcccat gcagcggcgc acgaccggag caaggactca catcgggggc aaggacctgc
    11701 gagcgcatac cctgacgccg tgacgatgac tcccggccag cccgagcacc ccgacaccga
    11761 ccacctctca cccgcgaccc gcgtcgtcgc cctcgggcgn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnna gcaccccgac accgaccacc tctcacccgc gacccgcgtc
    11941 gtcgccctcg ggcgtgaagc cgccgccccc ggccagcagg tcggggcgcc actcgtgctc
    12001 agctcgacgt atcacgccgg cggcgaggtc atgtacgccc gctcgggcaa cccgacgtgg
    12061 agcgccttcg agcaggccct gggcgatctc gagggcggtg atgcgctggt gttcgcctcc
    12121 ggcatggccg cggtgtcggc cgcgctgtcc ctgctccccc acggcggcac cgtcgtcgcc
    12181 cccgacgccg cctacaacgg tgtcgtcgcg accctggaag cccgcgaggc cgaagggtcg
    12241 gccaccgtgc gccgggtcga cgtcaccgac accgccgccg tgatcgacgc cctcgacggc
    12301 gccgacctgc tctggctcga gtcaccgacc aacccgctgc tcgaggtcgc cgacctgccg
    12361 gcgctgctcg gcgccgcccg cgagcgcggg gtgctctcgg tcgtcgacaa caccttcgcc
    12421 actcccctgc tgcagcgccc cctggagcac ggggccgatg tcgtcgtgca ctcggtcacc
    12481 aagtacctcg ccgggcacag cgacgtcctg ctcggtgccg tcgtcacggc cagcactccg
    12541 accggccacg ccctccacga gcgcctgttc acccaccgca ccctcgccgg cgcgatcgcc
    12601 ggcccgatgg aggtctggct cgcgctgcgt ggactgcgca ccctgcacct tcgggtcgag
    12661 cgggcgcagg ccagcgcccg tgaactcgcc acccgcctgg cgagccatcg cgcggtgacg
    12721 cgcgtgcggc atccgggatg gggcgggatc gtgtcgatcg aggtcgcggg cggggcggat
    12781 gccgcgcagc gggtgtgcga gtcgacgcac ctgtgggtgt actcgacgag cctgggtggc
    12841 gtggagagcc agctggagcg gcgacgccgc atcccgagcg agccaccgca ggtgcccgag
    12901 aacctcgtgc ggctctcggt cggcatcgag gacgtcgacg acctctggaa cgacctgagc
    12961 caggccctcg accaggcctg acccggtcag gcggtttcgg ctttgcgggg gcgcgagagc
    13021 agggccgaga cgacctcctg ggcgctgcgg tcgtgctgga tcatcgcgtc gacctgctcg
    13081 atgatcggca cgtcgacccc ggtgtgccgt gccagctcga ggatcgaggt gcacgacttc
    13141 acaccctcgg ccgtctgctt cgtgacggca atgacctccg agacggccag gccctgcccg
    13201 aggcgcaccc cgaacgagtg gttgcgcgac agcggcgaca tgcacgtcgc gatgaggtcg
    13261 cccaccccgg cgagcccggc gaacgtcggg gcctccccac cgagcgccat gccgaggcgc
    13321 atcgtctcgg ccaggccgcg ggtgatgatc gaggccttgg agttgtcgcc catgcccatg
    13381 ccctcggcca tgccgacggc cagcgcgatg acgttcttca ccgcgccgca gatctcggtt
    13441 ccgacgacgt cggagccggt gtagggccgg aagtacggcg ccgcgcaggc agcggcgacg
    13501 agctcggcca tcgccgggct cgacgacgcg acgaccgagg ccgcaggctg cttgacggcg
    13561 atctcgcggg ccaggttcgg cccggacacg atgacgactc gctccggctc gaccccaccg
    13621 acctccgaga tcacctcgga catccgtcgg gtcgtcccca gctcgacccc cttcatgagc
    13681 gagacgatcg ccgccgacga cgggagcgcc gatccccaga cctcgaggtt ggcgcgcagc
    13741 gtctgggacg gcaccgcgag cacgacgatg tcggcacccg ccgcggcctc gaacgggtcg
    13801 gtcgtggccc ggatggtgct gggcaggcgc agctcgggca ggtagtcctc gttggtgccg
    13861 gcgttgatct ggtcgacgac ctcctgccgt cgcccccaca tcgtcacgct cgtgcccgca
    13921 tccgcgagca ccgacgcgta cgccgttccc cagcttcccg tgccgtacac cgctgcgctc
    13981 gtcatgaggc ctccttgagg ggtcgtggtt tgccgatggg ggtcacgccg gctgcgcggg
    14041 ggtcgaagcg gatgccgggg gcgcgctccc cgcgcacccc ctccaggtcg ttggtcaggg
    14101 ccgccatgat ccggtcggtg gcgtcctgga gcacctcggc cgtcagcggc aggcctcgca
    14161 ggtcgtcgag ctcgaccggg ggcccggcgt gcacgaccat cgtcttgcgc ggcagcaggt
    14221 gcggccagtg ccggtagggc ccgaggatct cgtgggcgcc ccacgtgacg acgggcacga
    14281 cggggcagcc ggtctccaac gccacccggg ccgcgccggt cttcccccgc atcggccaca
    14341 tgtcggggtc gcgggtgagg gtgccctcgg ggtagatcgc gacgaccttg ccggcccgca
    14401 cggcctcgac ggcggagcgg aaggcgtgga tggcccggcc cgactcgcgg tgcacgcgga
    14461 tctggtcggc cctggtgagg atccagccga cgacggggat gcggaagacc tcgaccttgc
    14521 cgaggaaccg gggcgagaag ccgtggtcga cgaggaagtg cccgaacacg aacgggtcga
    14581 ggtgggagta gtggttggtg acgacgacga aaccaccctc ggtcgggaag ttctcctgac
    14641 cggaccagcg acgcttggtg aagaccatca gcagcggccg cagcacggcg acgacaaagc
    14701 ggtaggcacg aggcctggag tggtccgtcg ggatgctcac gcgctgcctt ccgtgcgggc
    14761 gggtcaccgc attgtggtct gcgacagcgg cgatgtcgag catgacgcca cgtgttggca
    14821 ggatcagcgc gtgcccgatc tcgttccgca gtgggcggcc gtcgtcccag tcaagggtgg
    14881 gccgctggcc aagagccggc tggccctgcc cgagcccgcc cgcagggacc tcgcgaacgc
    14941 gttcgcccac gacaccgtct ctgcgttgct cgacgcgatc gaggggatgc cggtgctcgt
    15001 cgtgacctcg gacccgacgg tctcctcgtg ggtcacccct gctggtgcgc ggctcgtccc
    15061 cgaccccggg ctgggtcttg acgcggcggt ggcggccgga tgccgtgtcg cagcggcggc
    15121 cggagccacc cgtgtcgcgg tggtgctggg cgaccacccc gcgctgcgcg ccgcggaggt
    15181 gagggtcgcg ctcgaggcga ccggacggca tccggcagcg gttgtccccg acgctgacgg
    15241 cctgggcacg gcgatgttga ccctgaccgt gccccggggc gagtcgatgg ccggggtgcg
    15301 gacggcgttc ggtgccggca gcgccgccgc gcacgaggcc ctgggccacg tgcggctcga
    15361 cctcgaccta ccaggactgc gggtcgacgt cgacgacgcc cggtccctcg cggaggccac
    15421 ccggctcggg ctcggcccac acagcgcccg ggcactggct cgcgctacgg ttcacggtgt
    15481 gcaggccacc atccaccgca tcgccgacga cggctccggc tcggccctgc tcgacgacgg
    15541 cgtggaggtc gacctgccac ctgacgccgc gcagcgcagc gggctgcgcc acctgcgggt
    15601 gggccagcgg gtgagcatcg aactcgacga gagtggggct gcggcgaccc gggtgtggat
    15661 caccgggatc gggcccggcg aggacatcca ctgaggcagt ggcgcccgac tgcgctgccc
    15721 tgatcgggtc gtcaggggag cttgtagacg gccccgaggt tgtcgagctt ttcggagaag
    15781 cgctcgtacc cgcggtggat gaggtcgatc ccgtgcaccg tggagcggcc ctcggccgcc
    15841 agggcggcga tgacgtagct gaacccgccg cgcaggtccg gcacggtgat ctcggcagcc
    15901 tgcagcgggg tcgggccgga gatgacggcc gagtgctggt agttcgcccg cccgaaccgg
    15961 cacggcgagc cgcccaggca ctcgcggaag gtctggatgt gtgccccggc ctcgacgagg
    16021 gcgtcggtga agccgaggcg gttctcgtag acggtctcgt ggacgatgga caaccccgtg
    16081 gtctgggtga gtgcgacgac gagcggctgc tgccagtcgg tcatgaaccc ggggtgcacg
    16141 tcggtctcga tggcgatgga gttcagcgtg cccccggggt gccagaagcg gatgccgtcg
    16201 tcgtcgatct cgaacttgcc cccgatgcgt cggaagacgt tgaggaacgg catcatcggc
    16261 agctgctggg cgccgcggac gtagatgtca ccacgggtgg ccagggccgc gcaggcccag
    16321 gagccggcct cgatgcggtc ggggatcgcc aggtggtcga agccgccgag ccggtcgacc
    16381 ccctcgatga ggatgacccg gtcggtctgg accgagatga tcgcgcccat cttctgcagg
    16441 atggcgatga ggtccatgat ctcgggctcg atcgcggcgc cccgcagcgt cgtgcgaccg
    16501 atggcgcgca cggcggtcaa cagcacctgc tcggtggcgc ccacactcgg gtacggcagg
    16561 tggatgtcag cggcatgcag tccgttgggc gcggtcaggc gcaggccgtc ctggcgcttc
    16621 tcgaccaccg ccccgaaggt gcgcagcacg tcgaggtggt agttgatggg gcgttcgccg
    16681 atgcgacagc ccccgaggtc ggggatgaac gcctcgccga ggcggtgcag caacgggcca
    16741 cagagcagca cggggacccg ggaggatccg gcgtgggcgt cgatgtcggc gatgtgggcc
    16801 tgttcgacgt tggtcggatc gaggaccagt tcaccgtcac gatcggtgga gtcgatgacg
    16861 acgccgtgca gctcgagcag gccgctgacg atctgcacgt cggagatctc gggcaccccg
    16921 cgcaggcggc acccctcctc ggcgagcatc accgcgacca tggccttgga caccaggttc
    16981 ttggcgcccc tgacctcgat gtcaccgacg agcggggagc cgccgtcgac gacgagggat
    17041 gaggtcatgg gccaagccaa acacacccgc gcaccgggtg cgacacaacg atcaccggca
    17101 tctcgcagtc agatcaggcg gacgccgact tcttggccgc ggccgtgcca gcgggggcac
    17161 gaccggtcgc ggtgccgtcc tcgggcaggg tgtgcggctc cttgacgtag gacttgaacg
    17221 cggtgccggc ccgaaaccgc ggcgccttga cctcggggat gggcaccgag gtccccgtgt
    17281 gcgggttgcg gccggtgcgg ggagctcgtt cgacgcgatc gaacgtgccg aaaccggtga
    17341 tcgagacctt cccaccccgg gccacttccc ggatgatggt gtcgagaacg gcatccagtg
    17401 cttccgctgc cgctctgcgg ctgccgaacg tcgactccag ttccttgacc agatctgcct
    17461 tgttcacgtg gacgtacccc aactcctgaa gagcgcgccg ggcgcagcgc aaccgcgaac
    17521 cgccgaaatg accggttcgg caccgaccgt agggccatcg aacgccgtcg tccaccgcta
    17581 gcggcgcgtc gtcgggcgac gtcggacgcc tctgcgaggg caccccaacg gggccgtgcg
    17641 ctcggttcgg ggtcgactgc ggcagtgacc cgctcgcgca ctcaggcggt gacggagggc
    17701 ttccagctcg gacgcgaacc ttcgaaagta gaaatgtcac cctcgtgacg aagggtgaga
    17761 ctgatgtcgt cgtggccctc gagcaggcgc caccgcgtgt agtcgtcgac ctcgaatcgg
    17821 gccacgagat cgccggcggt gatcgtgcgt gacaccaggt cgacggtgac cgacgtgccc
    17881 gggtggttct cgagcagctt ccacagcagt tcgacgtcgt cctggctgca ctgggccgtc
    17941 agcagcccct gcttgccgct gttgccacgg aagatgtcgg cgaaccgaga ggagatgacg
    18001 acccggaagc cgtagttcat cagcgcccag accgcgtgtt cgcgcgagga cccggtgccg
    18061 aagtccggcc cggccacgag caccgacccg gcggagtagc tcgggtcgtt cagcacgaac
    18121 gtctcgtcgc cacgccaggc ggcgaacagg ccgtcctcga agccggtgcg ggtgacccgc
    18181 ttgaggtaga cggccgggat gatctggtcg gtgtcgacgt tgctgcggcg cagtggcacg
    18241 ccgacgccgg tgtgggtggt gaaggcttcc atcagttgct ccctgcggtc acgggctcga
    18301 gatcggccgg gctcgacaac gtcccccgca cggcggtggc cgccgcgacg agtgggctca
    18361 cgaggtgggt gcgcccgccc ttgccctgac gaccctcgaa gttgcggttg gaggtcgacg
    18421 cgctgcgctc ccccggcgcg agctggtcgg ggttcatgcc cagacacatc gagcacccgg
    18481 gcaggcgcca ctcggcaccg gcggccgtga agacctcgtg cagcccctcg ctctcggcct
    18541 ggagccgaac ccgagccgat ccgggcacga cgagcatccg caccccgtcg gcgacctgac
    18601 gcccctggag cacggaggcc gcggcccgca ggtcctcgat gcgcccgttg gtgcaggacc
    18661 cgaggaagac cgtgtcgacg tgcacctcgc gcagcggcgt gccggcctcc aggcccatgt
    18721 actccagggc ccggcgggcg ttggccttgt cgttctcgtc cggcatcagc tcggggtccg
    18781 gcaccgccga gccgagcggc gcgccctgac cggggttggt tccccacgtc acgaaggggg
    18841 tgagtgccgc ggcgtcgagg acgacctcgt cgtcgaagac ggcgtcgtcg tcactgcgca
    18901 gctcgctcca ggcggcgacg gcggcatccc agtcggcgcc ggtcggggcg tggtcacgcc
    18961 cgcgcaggta ctcgaaggtc gtctcgtcgg gggcgatcat gccggcgcgg gcaccggcct
    19021 cgatcgacat gttgcacacc gtcatccggg cttccatcga cagcgcacgg atggcgctcc
    19081 cccggtactc caggacgtag ccctggcctc cgccggtgcc gatgcgcgcg atgacggcga
    19141 gcacgatgtc cttggccgta acaccgtcgg cgaggtcgcc ctcgacggtg atggccatcg
    19201 tcttgaacgg cttcaggggc agcgtctggg tcgcgagcac gtgctcgacc tcggaggtcc
    19261 cgatgccgaa ggccagggcg ccgaaagccc cgtgcgtcga ggtgtggctg tctccgcaca
    19321 cgatcgtcat gcccggctgg gtcaggccca gctgcggtcc gaccacgtgc acgatgccct
    19381 gctcggcgtc ccccatcggg tagagcgtga cgccgaactc ctcgcagttg cggcgcaggg
    19441 tctcgacctg ggtccggctc accgggtcgg tgatcggccc gggggtggtc gggacgttgt
    19501 ggtcctcggt cgcgagggtc aggtcaggcc gccgcaccgg tcgcccggcg agtcgcagcc
    19561 cgtcgaacgc ctgcgggctg gtcacctcgt gaagcaggtg caggtcgatg tagagcagat
    19621 cgggctcgcc ctccccgcgg tgcaccacat gggactccca gaccttctcg gccagagttc
    19681 cggccattgc tccacctcca cgacgtgacc gccgctgggc ggtctcacga cttcgacgca
    19741 tgcggacgcc cgcgtcgggc gccatcgctg tccaccgaca ttcgcacggg cagatggcca
    19801 acgaatgtgc gtctcaggca atgagacgct aatatcaaga catggacaac acaagtggcg
    19861 tcggggtgct tgacaaggcc gcgatcgtgc tcagcgccct cgaggccggc ccgtcgacgc
    19921 tggcccagct cgtgacggca accgggctgg cccgcccgac ggcccaccgg ttggccgtcg
    19981 ccctcgagca ccaccgactg gtcacccgcg acctgcaagg tcgcttcatc ctcggccccc
    20041 gtctcggcga gctggctgcc gccgcgggcg aggaccgcct gctcgccgct gcgggccccg
    20101 tcctgggtgc gttgcgcgac cacaccaacg agagcgccca gctcttccgg cgccagggtg
    20161 agcaccgggt ctgtgtctcc gcggcggagc gcccggtcgg cctgcgcgac tccatcccgg
    20221 tcggggcagc actgtcgatg cgcgccggtt cggccgctca ggtcctgctg gcctgggagg
    20281 agccggaccg gctgcaccgc ggcctccagg gagcagcctt ctccgcgacg tcgctgtcgg
    20341 gggtacggcg ccgcggctgg gcacagagcg tcggcgagcg cgagccgggc gtggcctcgg
    20401 tgtcggctcc ggtgcgctcc ccctccggcc gggtcattgc cgcggtctcc atctccgggc
    20461 cgatcgagcg gttgtcgcgc cagccgggcc ggctccacgc cgcgaccgtc atcgcgggcg
    20521 cgaacaagct caccgaggtg ctcgcccgcc acgccgcgca gcaggacgcc cagaacaacg
    20581 cctgacccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga cgtccgtcgc
    20701 gcgaggaggt tgcccgtgtc cctcaccact gcccagcgca tcgcccgtga ccgcacccgt
    20761 ctgctcgggt tcccgaggcc cggccgccgc gcgatcgtcg tcggcggggg cgcgatggcg
    20821 gcgcgacggg ccgcggccct gacccgggcc accacccccg tcacggtgtt cgccccggcc
    20881 ctgtgtgacg acgtgttcga cctcctggcc gagcgcctcg tgacctggga gaaccgttgg
    20941 ccggtcgagg ccgacctcgc cgacgcctgg ctcgtgcacg ccgcaaccgg cgacccggag
    21001 ctggacgcgc gcatctgcct ctgggcgtcc acggtgcgcg aaggcggcct cagcgcctga
    21061 cgatccgctc ccgggcggtc tcaccgcggc ggcccaccgg cacggtcccg cccacgtcgg
    21121 ccaggccgaa gggtgccgga cccgcgtaca ggctctcggc agccgacacg gcatacgtct
    21181 cgtgccagat cgtcacggcg gaggggtcct gacgagcagc acggtggaag gcgagccatg
    21241 ccggcggccc gggggcggct ccgagcccgg tcgcccccct gcggatcacc gacgtcgtcc
    21301 ccgacgcaga cgtcgacggc cccgcaccgg agctggcgga gggtgtgcga gccctgcaca
    21361 cccggcacca cgagcgattc cgtgagggtg ggaaggcgac cctcaatggt cttctccccc
    21421 acccatggct atggtcgggg ccatgaggca gcaggcgttg cgtgacgggg cccactgcct
    21481 cgacgtcacc cacctgcctg accacgtgcc ctccggcgca gcgctgctcg cgatcgcgct
    21541 cggcgaggag gcccgcggca tccgcggcct cgacgtcctg cgtcgcagcg tcgaccgcgc
    21601 gccgcccgcc tgaccttgac ccagcccctc ccggctgttc cgtcaccctg acggagcggc
    21661 cgttccccgg tcgcgccctt cgagcgcgac gctgacctgt caaggacacc accatgaaca
    21721 cccctgtcac ttttgcgcgc cctgaagtac ctcccgccag ggcggatcac ctgcgagggc
    21781 tcgaggccac gctggacgag cagttccgat ccatgccgac cctgccggcc gacgacgtcg
    21841 ccgcggccca ccgcgcatcc gtcgagcgca ccctccacga ggtgcgcctg gcgctgcgga
    21901 tgtacgccga gggtcggtac ggaacgtgca tccggtgcgc cgaccccatc tccgtggtgc
    21961 ggctcgaggc acgaccgtgg tccacccgct gcatccgctg cgccgacctc gtcggatggt
    22021 gacccctggc gaaggcggcc gcccccgcca ggtcgtcgtc gtcggagcag gcatggtggg
    22081 cctgtccact gcctggttcc tccaggacca cggcatcgac gtcaccgtga tcgaacgcgg
    22141 tgacgtcgct gccggggcct cctggggcaa cgccggctgg ctgacccccg gcctgacggc
    22201 cccgctcccc gatccggccg tgctcgggca ggggctgcga gcgctgctcg acccgtcctc
    22261 gccggtggtc gtcccccgga tcccgagccc gcgactgctg cgcttcctca ccgggttcgc
    22321 acgccacagc accacgcggc ggtggcgtgc cggcatgagc gcctacctgc aggtgaacgc
    22381 catggccctg ggcgcgttcg accgcctggc cgagggcggg gtcagcgagc cgacccacga
    22441 ggcggacccg ttcctggcct gctaccgcac ccccgaggac cgtcggttcc tgctcgagga
    22501 actggaacag atggccggcg ccggtcagga cgtccgtttc gacatcctga ccggtgagga
    22561 ggcccggcgc tgcgaaccgg cgctgtcgcc caccgtcggg gcaggcatcc ggatccacgg
    22621 ccagcggtac atccacccgc cccggttcat ggatgccctc gccgcctcct tccgagcccg
    22681 aggcgggacg atcctgacgg gaaggacggt caccgggctc caagatcgcg gggagtcggt
    22741 cgaggtggct gacgacaccg ggatcacgca tcgggtcgat gccgtggtgg tggccagcgg
    22801 tgcagagctc ggcgacctga ccagggcgtt cggggtgcga cacctcgtcc aggccggccg
    22861 cgggtacagc ttcaccgtcc gcgcacgcca gctcccgtcc gggccggtgt acttccccgc
    22921 ccaacgcctc gcctgcaccc ctctgcggtc gcccaccggc gaccggttgc gcgtggccgg
    22981 gacgatggag ttccgctccc cctcggctcc cttggaccgt gagcggatcg acgtcatggc
    23041 agccgccctg gcaccgctca tggacgggct ggacctgcac gaacggcagg aggagtgggt
    23101 ggggtcccgc ccctgcacca ccgacggtct ccccctcgtc ggcggcacca ggtcgcgtcg
    23161 ggtcttcgtc gccggcgggc acggcatgtg gggcatcgtg ctcgggccgg tcaccgggca
    23221 gctccttgcc agcctcgtcg caaccggcca gctccacccc gcgctgaccc cgttcgaccc
    23281 cacccgctga cccggcaccg caggccggaa aatgccaaac cccccggcga accggggggt
    23341 ttggcttttg tagccccgac gggattcgaa cccgcgctac cgccttgaga gggcggcgtg
    23401 ctaggccact acacaacggg gcctcgtgct gcacccatcc gctcgagagc ggctgggcaa
    23461 cggagcagaa ccttacgcca cagcgaggtc tttctccgaa tcgcgcccgg cgacaatgcc
    23521 gggtgcgatg gctggggtac caggactcga acctagaacg gatgaaccag aatcaccagt
    23581 gttgccaatt acaccatacc ccatggggta tgccatctgc cgttgccgcg agggcgtggg
    23641 cttcaggcga accgaggcac gatcttagcg ggcacgttcg ccagcaccca aatcgccgac
    23701 cgccacaccg gctccgcggc cacctcagag ggtcgagcgc agggcgtcca accgggtcaa
    23761 cgtggccgtc ttgcccagga tctccatgga ctcgaacagc ggcggcgaga tccggcgtcc
    23821 ggagaccgcg gtgcgcagcg gcgtgaaggc gaccttgggc ttgagcccga gcccgtcgat
    23881 gaccgcggca cgcaacgccg cctcgatggc cggcgccgtc cacgtcgact ccgacgccag
    23941 gaccccgctg tgctcgtcgg gcagcgcctg gagcgccgtg gacgccgcgt cgaggacgcg
    24001 cacggcatcc tcacccagct gggcgcgggc atcatcggcg acgtcgacca tgtcgtccgg
    24061 ggcgaagaac ggtgcgacca acgcccctgc ctcggtgagg tgggcgatcc gcgtctggat
    24121 cagggccgcg acctcgcgca gtcgcccgag ctcgcccagc gaggggttgc cgctgaggat
    24181 gccgtcggcc tccaggtagg gcagcagtcg cgaggtgaag tcgtcgagct ccagggcgcg
    24241 gatgtgcatg ccgttgagcc agtcgagctt cttcaggtcg aagatcgggc cgaccgggtt
    24301 gacgtcggac caggagaacc gggcggagaa gtcctcgaac gtgaagacct ccacgtcctc
    24361 accctccggg cccttggccg gcgggtaggc cagcagggcg aggaagttga cgagggcctc
    24421 gggcaggtag ccctgctcac ggaaccaggt gaggcgagcg gcggggttct tgcgcttgga
    24481 gatcttcgag gagtccgggt tgcgcagcag gggcatgtgc gcgaacttcg gcggttccag
    24541 cccgagccac tggtagagca ggatgtgctt gggcgtcgag gagatccact cctcgccgcg
    24601 cacgacgtgg gtgatgccca tctcgtggtc gtcgacgacg acggccaggt ggtaggtggg
    24661 gaagccgtcg gccttgaaga tgacctggtc gtccgggcgc ggtgccgaca
//
LOCUS       DAOSOC010000021        24083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_2112_length_24083_cov_9.901157, whole genome shotgun sequence.
ACCESSION   DAOSOC010000021 DAOSOC010000000
VERSION     DAOSOC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 24083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24083
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2112_length_24083_cov_9.901157"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PL014_03205"
     CDS             <1..1185
                     /locus_tag="PL014_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT56129.1"
                     /translation="VAIGTTEFVTMGLLPEIAAGVGVDIPTAGHTISAYAAGVVIGAP
                     LLAVLGATWPRKALLVGLMVAFTIGNILSAIAPDYGLLVGARLLAGLPHGAFFGVAAL
                     VAVDLAQRGQAGRAVGRVMLGIPIANVLGVPGATWLGQAYGWRTAYVLVAVIGVATVA
                     LIAYAVPHLPADPTRTVRSELSALTSLQVWLTLGVGAVGFGGMFAMYSYIAPTVTEVT
                     GLPASAVPLFLLVFGVSGFIGNIVAGRMGDWSVIRSIVIGLVGMGLALGVFAAVASYA
                     VPALIVLALTSVLASVLVINLQLRLMQVAGSAQTLAAASNHAALNLANALGAWLGGLV
                     IAAGYGYRAPSVVGVGLAVLGLVILGISLLIHRRDHPGRVSIRPGGPAGRDASSSAAQ
                     VS"
     gene            1483..2280
                     /locus_tag="PL014_03210"
     CDS             1483..2280
                     /locus_tag="PL014_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOT56130.1"
                     /translation="MPSTLTCRVEGGVAQVRLNRPDKLNALTLDTLEDLVATARELTS
                     NRTLRGVVIAGEGDSFCAGLDFATVLKNPPRVARSFVPNPWRGTNLFQEACWAWRRVP
                     VPVVAAVHGHCYGGGLQIALAADLRMTTADAQWSVLEAKWGLVPDMSGIQALSQQVRM
                     DVAKRLTMTGEVVSGERAVDLGLASELHADPVAAATAFVEQVATRSPDAVAATKRLFG
                     RTWSSSDRRTFARERIEQAKLLATKNTTIAREAAMKRAVPVFDPRRR"
     gene            2277..4448
                     /locus_tag="PL014_03215"
     CDS             2277..4448
                     /locus_tag="PL014_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT56131.1"
                     /translation="MTSQPKTSPADRDVRHITCTLCEAMCGLEVQLDDSDAIVSMRGH
                     EEDPLSRGHICPKAFALQDLHDDPDRLRAPVVRTAQGTWKEVSWDEAITYAAQRLSAV
                     QAEHGDDALAVYLGNPNVHSLGALTHHPTLVRLLRTRNRFSATSVDQLPHHVASWALY
                     GHQYLLPVPDIDRTALLVLVGHNPMASNGSLWTVPDFPQRRRELAARSGRLVVLDPRR
                     TETAKIADEHHFVRPGSDAAVLLAIVRELLEGDLAAPAEYVHGMDRVRDAVAGFTPEL
                     AERVSGMSADAVHRLARDLAAAPSAAVHGRMGVSTQAHGVVCQWAVQAINILTGNLDR
                     PGGTMFTTPAVDLIGRGVLSPGGFGTKRTRVRGLPGFGGEFPVSAMAEEITTPGEGQV
                     RALLTIAGNPVSSTPAGHRLDEALAGLDAMVAIDFYVNETTRHADVILPPTSPLQRDH
                     YDIVFHLLAVRDTARWSPALFTPPVGAKHDWEIARDLGTALLRERGGGLKQRWSRATL
                     ETEARLRLTPRRQLDALLRSSGARLSIAKLEKTVAGADHGPLKPRLPGRLATADRRID
                     LLPDLVAQALPGLLTTLVSGPVLAEGELLLIGRRHQRDNNSWLHNSTRLIRGKPRHAL
                     LANPDDLAARGIHSGDVVVVRSAVGEVRVEVEASADLMPGVVSLPHGYGQAREGVRLR
                     NAVDVPGVSMNDLTDPSVVESVSGNAVLNGVPVTVEPAART"
     gene            complement(4528..5040)
                     /locus_tag="PL014_03220"
     CDS             complement(4528..5040)
                     /locus_tag="PL014_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56132.1"
                     /translation="MLDALRLWTARRWVAAATASVVTALVIALPTAMIPSPVFGREIP
                     TTWWAWPVLAVTSVLSGLLFATYVREPGGQESGEASINRGGAAGGLLAFFAVGCPVCN
                     KLALIALGYTGALQWFAPVQPFLGVAAIGLLAWALRARLRGQLACSLPAAPPNASAAD
                     LPTGKEPARS"
     gene            complement(5183..5767)
                     /locus_tag="PL014_03225"
     CDS             complement(5183..5767)
                     /locus_tag="PL014_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT56133.1"
                     /translation="MRSPSRVRMSPQERRRQLVGIGLRKFVEQPVQDLSLDEVAAEAG
                     VSRGLLFHYFPSKTEYHRAVVEAAWRRVGRNVAPDPDAVGSAAVRQVVERFLSQVERR
                     RDTYVALVLGQAPIAPGLAVGGLREQLAALVLGAADLPAAAMPAAHGWVAYLEDRALQ
                     WSALPEGARQAEREALAEHCTRALTLLTDPAPAA"
     gene            5808..7124
                     /locus_tag="PL014_03230"
     CDS             5808..7124
                     /locus_tag="PL014_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT56134.1"
                     /translation="MDFAPSPRAVDLAQRVRAFIDTEIEPVEPAMLADVAARRARGED
                     AWPPPAALADIAAKARAQGLWNLFLPAGHGDAHAERFGTDGGKGLTNTDYASIAEQMG
                     RSALAPLVFNCNAPDTGNMEVLLKYGTPEQQEQWLDPLLDGRIRSTFLMTEPGVASSD
                     ATNMQATATLDGDEVVLDGRKWWSTGAGHPDCRIGVFMGLTDPSAHRHRQHSMVLVPL
                     DAPGVTVERMLTALGQYDEPLGHGQVNLDAVRLPASNILAGPGRAFEIAQGRLGPGRV
                     HHCMRLIGLAEKSLELACERSTQRIAFGKPIANLGGNRERLARARIAINQARLHVLHA
                     AWKLDTVGIEGAQSEVSEIKAAVPQMACDVIDLAIQLHGGGGMSDDFPLAAAYAGARS
                     LRLADGPDEVHLGVVARHLLRPYAERAAAAIASTATASGSTAGGSR"
     gene            7121..7933
                     /locus_tag="PL014_03235"
     CDS             7121..7933
                     /locus_tag="PL014_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT56135.1"
                     /translation="MSRRVLVTGGASGLGLALTELMLDRGDTVLVVDLAEDRPDAVPE
                     GAAYRRLDVRSQQDWDAAMAWVRDTWGGLDLLVNNAGVATGGRIDVESMSDWERVVDI
                     NLLGVARGCHTFTPMFKAQKSGHIVNVASLAGLVHGPGMSSYNATKAGVVALSETLSF
                     ELAPWSIDVSVVCPAFFRTNLHQSFSGKDAAMQEAGVRLITQASVDAAHIAAIVLKGV
                     DARKRVILTDRLGRQAYYSKRFVRPLYDRMLTAQAKRLARRAGADPAELTRD"
     gene            7926..9008
                     /locus_tag="PL014_03240"
     CDS             7926..9008
                     /locus_tag="PL014_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HOT56136.1"
                     /translation="MTEALAEVRDEDAFDVERVAAWLRENASDAVDRAELTGLPEVRQ
                     FVGGASNLTYLLRFHSGRDLILRRPPFGTKAKGAHDMGREHDLQAALAGAFPLVPAMV
                     ASCNDESIIGSPFYVMERIEGTILRRDIPPELGLDVDGVRALCENAIDTLVRLHDVDV
                     TASGLAGLDRGDGYVARQVQGWTGRFRRAHTPDVGDFESTIAWLEANQPGDRPHVVIH
                     NDFRFDNLVLDPGDPTRIVGVLDWELATVGDPLMDLGSALAYWVQADDDPAFLAVRLQ
                     PTHTPGMLTRAEVVQRYCSARSLDVTPQEWLFYDVFGTFRLAGIAQQIYYRYHHGQTS
                     NPAYARFGDFVRILDARCSALLAGQP"
     gene            9056..9808
                     /locus_tag="PL014_03245"
     CDS             9056..9808
                     /locus_tag="PL014_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT56137.1"
                     /translation="MPTTLITGASSGLGAEMARQLADRGWDLALAARRTERLEELRDE
                     IVAKHPGRRVELRALDVTDDDQVFEVFRAFRDDLGVIDRVVVNAGLGKGAPIGTGRYD
                     ANRQTAMTNFVAALAQCEAAMEIFRDQKRGHLVMISSVSAMRGMRKTVTTYAATKAGV
                     AALAEGLRNEMLGEPDLDIRVSTIYPGYIRSEMNERVTKTPFIVDTRTGVAAMVDAME
                     KEKANALVPAWPWVPLGKAMRHLPLPLVRKIM"
     gene            9811..10776
                     /locus_tag="PL014_03250"
     CDS             9811..10776
                     /locus_tag="PL014_03250"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOT56138.1"
                     /translation="MGRVLVTAQVVGDAVARLREAGHDVVFRDIDAPMPRDEFLAALP
                     GVDALVAMLVDRVDAEVLDAAGPGLRVVANVAVGYDNVDVAACRERGVLVTNTPGVLT
                     DATADIAMSLILMSTRGLGAAERRVRTGTPWRWSLTAHLGVGLRGKTLGIVGPGAIGL
                     ATARRAKAFDMRVLLSGRSSPDPAVVGELDAAVLDLDELLTASDVVSLHCPLTPATRH
                     LIGARELRLMRPGAHLVNTSRGPVVAEAALVAALDAGEIAGAGLDVFEDEPRVHPGLL
                     GREDVVLLPHVGSATVETRTAMADLAVANVLAVLAGRDAVSPVGG"
     gene            11136..11531
                     /locus_tag="PL014_03255"
     CDS             11136..11531
                     /locus_tag="PL014_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56139.1"
                     /translation="MEIFNAAVADGAVARGDGTIAYALQNGSELVMAAPPTKTKGGSP
                     QDLGAGWDGWRGPYISFNRVDQSALLAGGVAALSVAVCAIPGVGWASCAALVGIVTIA
                     AYYVNEYGRCSTSKPNLRVYLSGRRGCYA"
     gene            11528..11797
                     /locus_tag="PL014_03260"
     CDS             11528..11797
                     /locus_tag="PL014_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56140.1"
                     /translation="MNGSSIGGFILTIAIPIVFLGASTLGWRRGGSRQRLLTFTLLGV
                     VLACLAFALIGRIVDVESFTMTFVVVMVGLSAGTVMIGKKRLNLA"
     gene            complement(11884..13215)
                     /locus_tag="PL014_03265"
     CDS             complement(11884..13215)
                     /locus_tag="PL014_03265"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOT56141.1"
                     /translation="MVVASDSFKGSLTSLQVGDAVRLGLLDVAPHAEVVVVPIADGGE
                     GTVAAAVAAGWRAVPVAVHGPTGLSVTATVAVRGGREDLAPIPVAEQGLIPRIGTAAP
                     QRSTADTRHRTPDPPPLQPEATPTVALVEMADVCGLGRLPDAVPAPLTASSRGLGEAI
                     RAALDLGAAEVIVGVGGSASTDGGAGMLAALGARLLDAGGVDLPDGGAALARLAHLDL
                     SGLDPRLAHTYIALAADVDSPLLGPTGAAAVFAPQKGATPAEVDLLEAALTHWADVVE
                     SHPDVPISTNRSLQGQEGGVPRVTDWSMGGRMPGAGAAGGIGFALMAVLGAHRRSGID
                     VVLDLVGLDAALSRADLVITGEGSLDAQTLTGKAVRGVATRATAHGIPVVAVCGRRIL
                     DDAGLTALGLRAAYPLTELEPDVRRSMEAAAALLRRTGQRIAHEETQPPAE"
     gene            complement(13244..13732)
                     /locus_tag="PL014_03270"
     CDS             complement(13244..13732)
                     /locus_tag="PL014_03270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT56142.1"
                     /translation="MVIRSIEPGDWPAFFPVFHDIVEAGETYAYPLGLTPEQAWDLWV
                     EEPPGVCVVAVDESGAVLGSAKAGPNRPARGSHVATASFMVSPAARGRGVGRQLGEWV
                     LTWAHEQGYAAMQFNAVVETNEAAGALWKDLGFRIIGTVPEAFESRTHGRVGLHVMHR
                     YL"
     gene            complement(13742..14176)
                     /locus_tag="PL014_03275"
     CDS             complement(13742..14176)
                     /locus_tag="PL014_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT56143.1"
                     /translation="MSEPSPVTTLTDDACWEMLHGQEFGRLAFHLADEVHLVPINYAV
                     DADRRIVFRTAEGSKLLGLTMNADVAFEIDEFTEDEATSVVIRGRARQLEHHEEAETE
                     LLPLRPWVNTAKFNTIVIEVDEITGRRFGLTRPWLHMRPQED"
     gene            14291..>14516
                     /locus_tag="PL014_03280"
     CDS             14291..>14516
                     /locus_tag="PL014_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOT56144.1"
                     /translation="MSPAQVVVIGAGPGGLAAAAALGARGVQALVVDRDSQVGSSWRR
                     HYERLHLHTPRRWSGLPGYRIPRRFGRWVAR"
     assembly_gap    14517..14616
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14617..14800
                     /locus_tag="PL014_03285"
     CDS             <14617..14800
                     /locus_tag="PL014_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT56145.1"
                     /translation="DTAANSARWLVHLDDGPPLAAEHVIVATGYNHTPVTPDWPGLDG
                     FTGDLVLARDYRNGRP"
     gene            15172..15327
                     /locus_tag="PL014_03290"
     CDS             15172..15327
                     /locus_tag="PL014_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56146.1"
                     /translation="MLDGRGLPVVHGAHEPPGAPGLWFTGFTNPISGMLRELRIDAER
                     IAAAIAR"
     gene            complement(15616..16926)
                     /locus_tag="PL014_03295"
     CDS             complement(15616..16926)
                     /locus_tag="PL014_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOT56147.1"
                     /translation="MSASAREFDIVLFGATGFVGRLTARHLAAEAPEGVRIALAGRSQ
                     SRLEDLARGLGSQAAGWPLLVVDATNPDAVADLARRAHVVVTTVGPYVKYGVPLAAAC
                     AEAGTHYCDLTGEVLFVHRSIAANHETAKRTGARIVHACGFDSIPSDLGVMLTAQAAK
                     ADGADLGRTHLAVRSMKGGFSGGTIDSARVQVDELKADPTTRRIVGDPWALAEGPRPG
                     RDRKVASESGATASGSSGSSGASGPAAVLAKLAKASPVKRDADNGHFTGPFVMAAFNT
                     RIVARSASLLGYGEGFRYVEYSDYGSGPTGAFAAGMTSVALGAGLAGMSFGPTRSLLD
                     RVLPKPGEGPSEQAQAAGRFRMEVTGEATNGARYRTTVAAPYDPGYSGTAVMLGQAAL
                     ALLQDGDALPDAAGVLTPATAIGMPLVERLREHKFTLDTVRLPG"
     gene            16978..18207
                     /locus_tag="PL014_03300"
     CDS             16978..18207
                     /locus_tag="PL014_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT56148.1"
                     /translation="MRTNPRVLAVVQAGGQGSRLDVLTRERAKPALSFAGSFQLIDVA
                     LTNCAHSGICDVWLSVQYQAGSLHHHLASGRPWDLDRTRGGLRWLMPQQGGGSAAQDG
                     FSNGNGDDLHRFSDAIREFAPDAVVVMSTDQVLALDLRPVVAAHLERGSHCTVVTTEV
                     SLTQAADKAVITIGRGSRVARLDYKPERPSGTTIAAEVFVYDPTVLLATLEDLRRELS
                     HTDDGNTGIGDFGEHLLPRMIRDGVVHAWPHEGYWADLGTPAAYLSAHRDLLAGRVDV
                     LDRPDWPLLTRWPELPASRVDTGAVLEDFVLSAGCRVKGTVRRSVLGPGVVVEPGAVI
                     EDAVLLAGVRVSRDARVVTAILDEGVRVEVGARVGQATRATRAHDHHIAVVGRDSVIG
                     RGVTVPAGARLEPGTTA"
     gene            complement(18412..19845)
                     /gene="xylB"
                     /locus_tag="PL014_03305"
     CDS             complement(18412..19845)
                     /gene="xylB"
                     /locus_tag="PL014_03305"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOT56149.1"
                     /translation="MPTDNQTDRPLVAGVDSSTQSCKVVVCDAVTGEVVRTGRAGHPD
                     GTEVDPRHWWSAWQEASGGGILDGVSAIAVGGQQHGMVLLDESDAVVRPALLWNDTRS
                     AGSADDLVAELGAQAWADATGVVPVAATTVSKLRWMADHEPENLARASTCVLPHDWLT
                     GRILGEGSSGPRAWTTDAGDASGTGYWSAGTRSYREDLLALATRGADLAVPRVAGPSE
                     VVGHTASGLAVAAGTGDNMGAALGLGLVPGDVVVSLGTSGTAFARTDVASADPSGSVQ
                     SFADAEGGFLPLVCTLNAARVLGAGAAMLNTDHAGLDRLALAAAPGAGGLTLLPYLDG
                     ERTPNLPTATGSLHGLTRSNATPENLARAVVEGMLLGLAAAVDAVRAAGCPVHRVLLI
                     GGAAASVAVQEVAADVFGVPVAVPGAGEYVAVGAARQAAWALAGGEAPPVWDVAVDVT
                     VEPDEAVRDRAAEVRGRYAATVAATYP"
     gene            complement(19863..20912)
                     /locus_tag="PL014_03310"
     CDS             complement(19863..20912)
                     /locus_tag="PL014_03310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT56150.1"
                     /translation="MARRRALSTAALAATAALVVSAAPAHAANPSYVALGDSYSSGTG
                     TRSYISDGTSCLRSVYAYPSLIASAKGYDLNFRACSGAKIADVSNTQLSALSSSTAYV
                     SISIGGNDAGFASVLTTCAQPAWLSNCNGAIDKAQAYVNQTLPGALNTLYANIKARAP
                     QAKVTVVGYPRIFNGVDCNLLTWFSSSEMTRLNAMADLVNSTTRARATAAGFSFADPT
                     TPFVGHAVCSSSEWINGLSNPIQESYHPNRTGQASGYTPTVSPYLTGSTVTATAALLT
                     TAKGSTKRLADQQRRYAASDRAIKPALVLAPDLSTPAAKAAAKKAGVDLSSRASIDAV
                     DRVYSARQAAEFAAR"
     assembly_gap    20943..21042
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21063..21953
                     /locus_tag="PL014_03315"
     CDS             21063..21953
                     /locus_tag="PL014_03315"
                     /inference="COORDINATES: protein motif:HMM:NF018677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HOT56151.1"
                     /translation="MALTDLSRCSVASRHRGDPLAGSAPVASRWLLIEHQGPWAKKPL
                     ATPPLTASLGAEIDRICASFGGKVLLVRRPGRRLPGEDPHSWFAIDTIRRTRVRGSWR
                     TADDLREAAHALGMPLSDSDEDADPMVLVCTHATRDACCAVRGRPIAATLAKALPEEV
                     WECTHLGGHRFAGTLLSLPDGACFGQLDPDNALAAVMGHRAGRTDARFLRGTTRWDPP
                     VQAALAAVLAEHGPADLDDLAPGAADTDGDHTTVEVYGRGSLPARAVVDVVREALPDA
                     PLSCGDVPKAHVAHRAVLRP"
     gene            complement(21955..>24083)
                     /locus_tag="PL014_03320"
     CDS             complement(21955..>24083)
                     /locus_tag="PL014_03320"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOT56152.1"
                     /translation="MPARRGRPRTAVLSATVAVVALVGGWTWAAALQPGGFDARSETI
                     SALAASATPHRWVMTAALVVTGLAHVVTAWALGAAQRTGRLILAAGGVATLFVAAVPL
                     PSRAESSAAHTAVATASFVLLGLWPWFAATESGPRVLTQRVARTAAMVLTASVASLGL
                     GLSSASFGLHERVVAALTVIWPLVTAIGVWWWAGHRIGSRRVLQGLGVLAVTVACIAA
                     GVIATTVAPATAHTRNYQATVSLDPDPTHLGQILATTAFGDLELGFSGVAPGIRSVPQ
                     VNASIADELSRPNVSLASLRPGPAELSEAIREVAVDVLLRFAIGGLVLIGAVVGADAV
                     LRRRRPRLTLVVACGTGWVLATALTAASLYATYQPQRQETFTSTGILGTLQRNQGILS
                     DVEARATQVAPYLRNVIALSTALQQKYSAAPLEADTALRALLVSDIHGGNQYSLMRTI
                     VQEESVNVVIDAGDLLNFGTVAEGEAAGIFSGIESLEVPYLFVRGNHDATSATDTAVL
                     ARLAQVPNVVLLEPTPGEYTEASIGGVTISGFNDPRWFGDSGTGSPAKQVPAREAFVA
                     AFGGRTASDVVVSHEPWALKGVEGGVLVNGHMHSTDLEGNRVQVGTFTGGGPVNHFVE
                     QAPGEELVGQPSAFDVLTFGTNCRLATLTRYRYSNVIEGRPSYDGVQLVNGSRIDRRE
                     VDPERVCSRDEPLTTATVLPASAPSP"
BASE COUNT         3321 a         8605 c         8544 g         3413 t
ORIGIN      
        1 gtcgccatcg gcaccaccga gttcgtgacg atgggactgc tgcccgagat cgccgcgggc
       61 gtaggggtcg acatccccac cgccgggcac accatctccg cctatgccgc gggggtggtc
      121 atcggtgcgc cgctgcttgc ggtgctcggg gccacctggc cgcgcaaggc cctgctcgtg
      181 gggctcatgg tggccttcac catcggcaac atcctgtcag ccatcgctcc tgactacggc
      241 ctgctcgtcg gggcccggct gcttgcgggc ctgccgcacg gcgcgttctt cggggttgcc
      301 gccctcgtcg ccgtcgacct cgcccagcgc ggccaggccg gtcgcgccgt cgggcgggtc
      361 atgctcggca tccccatcgc gaacgtcctc ggcgtgccgg gagccacctg gctcggccag
      421 gcctacggct ggcgcaccgc ctacgtcctc gtcgccgtca tcggggtcgc gaccgtcgcc
      481 ctcatcgcgt acgccgtgcc gcacctgccc gcagacccca cccgcaccgt ccgcagcgag
      541 ctgagcgccc tgaccagcct ccaggtgtgg ctcaccctcg gcgtcggcgc tgtcggcttc
      601 ggcggcatgt tcgcgatgta ttcctacatc gccccgacgg tcaccgaggt gacgggtctg
      661 ccggcatccg ccgtaccgct cttcctgctc gtcttcggcg tctcggggtt catcggcaac
      721 atcgtcgccg gacgcatggg ggactggtcg gtcatccgct cgatcgtcat cggcctggtc
      781 gggatgggcc tcgccctcgg ggtgttcgcg gcggtggctt cgtatgccgt gccggccctg
      841 atcgtcctcg ccctcacctc ggtgctcgcg tctgtcctcg tcatcaacct gcaactgcgc
      901 ctcatgcagg tcgcggggtc ggcgcagacc ctggccgcgg ccagcaacca cgccgcgctc
      961 aaccttgcga acgccctcgg cgcctggctc ggcgggctcg tcatcgcggc cggctacggc
     1021 taccgggcgc cttcggtcgt cggagtgggc ctggcggtgc tgggtctggt catcctcggc
     1081 atctcgctgc tcatccaccg gcgcgaccac cccgggcggg tgtcgatccg acccggtggc
     1141 ccggccggtc gggatgcgtc ctcctcagcg gcgcaggtct cctgacgcac tcgggactac
     1201 ggcgcccggt cggacaccca cctcgtgccg gtgcccgacg agatcatgtg agaggtgtcc
     1261 gtgctcgacg agatcctgag agagagggcg taagggtgcc gttagagccc cttgcgcagg
     1321 gcaagggatt ccttgcgggc ggcaaggaat cctgacagca cccccacgac ctctctcagc
     1381 cgcgaacgga agtccgcgcc agcgtggatc gtcacgacga cgcagggtga tgcgacacac
     1441 gcaaccgcga cggcatccgg tggtggtccg tagggtggcg gtatgccgag caccctcacg
     1501 tgccgggtcg agggcggcgt cgcccaggtc cggctgaacc gccccgacaa gctcaacgcg
     1561 ctgacgctgg acaccctcga ggacctcgtc gccaccgccc gtgagctcac ctcgaaccgc
     1621 accctgcggg gggtggtcat cgccggcgag ggcgactcgt tctgcgccgg gctcgacttc
     1681 gcgacggtgc tcaagaaccc gccgcgggtc gcccgatcct tcgtgccgaa cccctggcga
     1741 gggaccaacc tgttccagga ggcttgctgg gcgtggcggc gggtgccggt cccggtcgtg
     1801 gcggccgtgc acggtcactg ctacggcggc ggcctccaga tcgccctcgc cgcagacctg
     1861 cggatgacca ccgccgacgc gcagtggtcc gtgctcgagg ccaagtgggg gttggtcccg
     1921 gacatgagcg gcatccaggc gttgtcccag caggtgcgca tggatgtcgc caagcgcctc
     1981 accatgaccg gcgaggtcgt gagcggtgag cgcgccgtcg acctcgggct ggccagcgag
     2041 ctgcacgccg acccggtcgc cgcggcgacc gccttcgtcg agcaggtcgc gacccgcagc
     2101 ccggatgccg tggccgccac caagcgcctc ttcggccgca cgtggtcctc ctccgaccgc
     2161 cgcacgttcg cgcgcgagcg gatcgaacag gccaagctcc tcgcgacgaa gaacacgacc
     2221 atcgcccgcg aggccgccat gaagcgggcc gtcccggtgt tcgaccctcg tcgccgatga
     2281 cgtcgcagcc gaagacgtca ccggctgacc gcgacgtccg gcacatcacc tgcaccctct
     2341 gcgaggcgat gtgcgggctc gaggtgcagc tcgacgactc cgacgccatc gtctcgatgc
     2401 gcgggcacga ggaggatccg ctctcgcggg ggcacatctg ccccaaggcc tttgcgctcc
     2461 aggacctcca cgacgacccc gaccgcctgc gtgccccggt ggtgcgcacc gcccagggga
     2521 cctggaagga ggtgtcgtgg gacgaggcga tcacctacgc cgcccagcgg ctcagcgcgg
     2581 tgcaggccga gcacggggac gacgcgctgg cggtgtacct cggcaacccg aacgtccaca
     2641 gcctcggcgc cctgacccac caccccacgc tcgtgcggct gttgcgcacg cgaaaccggt
     2701 tctccgccac ctccgtcgac cagctgccgc accacgtcgc gtcgtgggcg ctgtacggcc
     2761 accagtacct gttgcccgtg cccgacatcg accgcaccgc gctgctcgtg ctcgtcggcc
     2821 acaaccccat ggcctcgaac gggtcgctgt ggaccgtccc cgacttccct cagcgccgtc
     2881 gcgaactcgc cgctcgctca gggcgactcg tcgtgctcga cccgcgccgc accgagaccg
     2941 ccaagatcgc cgacgagcac cacttcgtcc gcccgggctc cgatgccgcg gtgctgctcg
     3001 ccatcgtgcg tgaactgctg gagggcgacc tcgccgcccc ggccgagtac gtgcacggca
     3061 tggaccgggt gcgcgacgcg gtggccggtt tcacgccgga actggccgaa cgggtgtccg
     3121 gcatgtcggc ggatgccgtg caccgcctcg cgcgggacct cgcggccgcg ccgtcggctg
     3181 cggtccacgg gcggatgggg gtgtcgacgc aggcccacgg ggtggtgtgc cagtgggcgg
     3241 tgcaggcgat caacatcctc acgggcaacc tcgaccggcc cggcggcacg atgttcacga
     3301 ccccggccgt cgacctcatc gggcgggggg tgctctcgcc agggggcttc ggcaccaagc
     3361 gcacccgcgt gcgcggcttg cccgggtttg gcggcgagtt cccggtgtcg gccatggccg
     3421 aggagatcac gacccccggt gagggccagg tgagggccct gttgacgatt gctggcaacc
     3481 cggtgtcctc gacgccggcc gggcaccggc tcgacgaggc actggcaggc ctcgacgcca
     3541 tggtggcgat cgacttctac gtcaacgaga ccactcgcca cgccgacgtc atcctgccgc
     3601 cgacgagccc gctccaacgc gaccactacg acatcgtctt ccacctgctc gcggtacggg
     3661 acacggcccg ctggtcgcct gcgctgttca ccccacccgt cggtgccaag cacgactggg
     3721 agatcgcgcg cgacctcggc accgccctgc tgcgggaacg cggcggtggc ttgaagcagc
     3781 ggtggtcgcg cgccaccctc gagaccgagg cgcgtctgcg cctgaccccc cggcgccagc
     3841 tcgacgcgct gctgcgctcc tccggggcgc ggttgtcgat cgccaagctc gagaagacgg
     3901 tggccggggc cgaccacggc ccgctcaagc cgcgactgcc gggtcggctc gccacagccg
     3961 accggcgcat cgacctgttg ccggacctcg tggcgcaggc gctgccgggc ctgctcacca
     4021 cgctggtgtc cggcccggtg ctggcagagg gtgagctgct gctcatcggc cgtcgccacc
     4081 agcgcgacaa caactcctgg ctgcacaact cgacccggct catccgtggg aaaccccgcc
     4141 acgcgctcct ggcgaacccg gacgacctgg ccgcgcgcgg catccacagc ggtgacgtcg
     4201 tcgtcgttcg gtcagcggtc ggtgaggtgc gcgtcgaggt cgaggcgtca gccgacctca
     4261 tgcccggggt cgtgtcgctg ccgcacggct acgggcaggc gcgggaaggg gtgcggctgc
     4321 gcaacgccgt cgacgtgccg ggggtgtcga tgaacgacct caccgacccg tccgtcgtcg
     4381 agtcggtgtc gggcaacgcg gtgctcaacg gcgtgccggt gacggtcgag ccggcggcgc
     4441 gcacctgacc gaggcggcac aggccgggcc tgccaaccgg caaaggcgca cacgtcggct
     4501 gctgctgagc agggccgacc ccgggaatca ggaccgggcc ggttctttgc cggttggcag
     4561 gtccgccgcg ctggcgttcg gcggggctgc cgggagtgag caggcgagct ggccgcgcag
     4621 ccgtgctcgc agcgcccacg cgagcagccc gatcgctgcc accccgagga acggctggac
     4681 cggcgcgaac cactgcagcg cgccggtgta ccccagggcg atgagcgcga gcttgttgca
     4741 caccgggcag ccgacggcga agaacgcgag gaggcctcct gcagcccctc cgcggttgat
     4801 cgacgcctcg ccgctctcct gtccgccggg ctcgcgcacg taggtggcga agagcaggcc
     4861 actcagcacc gaggtcacgg cgagcacggg ccaggcccac cacgtcgtcg ggatctcgcg
     4921 gccgaagacc ggcgacggga tcatcgccgt cggcagggcg atgaccaggg cggtcacgac
     4981 ggatgccgtc gcagccgcca cccagcgtcg tgcggtccac aggcgaaggg cgtcgagcat
     5041 gcggagaata taccccactg ggtatctttc gcggttggca tccagggagg gcagtcgagg
     5101 agtcgagaca cgcccgggat ccgcgacgga gccctgcatg tctcgacccc tcgactgcgt
     5161 caggcggcgg gggtgggatc ggtcaggcgg cgggggccgg atcggtcagc agcgtcaggg
     5221 ctcgggtgca gtgctcggcg agagcctcac gttctgcctg gcgcgcaccc tccgggaggg
     5281 ccgaccactg cagggcccgg tcctcgaggt aggcgaccca cccgtgcgct gcgggcatgg
     5341 cggcagccgg caggtccgcg gccccaagca cgagcgcggc cagctgctcg cgcagcccac
     5401 ccacggccag ccctggagcg atcggcgcct gcccgagcac gagcgccacg tacgtgtccc
     5461 gccgccgctc gacctggctg aggaagcgtt cgacgacctg gcgcacggca gccgacccga
     5521 cggcatccgg gtcgggagcc acgttgcgcc ccacccgacg ccaggccgcc tcgaccaccg
     5581 cgcggtggta ctcggtcttg ctcgggaagt agtggaagag cagcccgcgc gagacgccgg
     5641 cctcggccgc gacctcgtcg agcgagaggt cctgcacggg ctgttcgacg aacttgcgca
     5701 gcccgatgcc gacgagctgg cggcgacgtt cctgcgggga catgcgcacc cgtgacggcg
     5761 atctcacgct attgagcatt gctcaacaac cctctagggt caaggacatg gacttcgccc
     5821 cgtccccccg tgctgtcgac ctcgcccagc gcgtccgcgc gttcatcgac accgagatcg
     5881 aacccgtcga gccggccatg ctcgccgacg tggccgcccg ccgcgcccgc ggtgaggacg
     5941 cctggccacc cccggcagcg ctcgccgaca tcgccgccaa ggctcgcgcg cagggcctgt
     6001 ggaacctctt cctgcccgcc gggcacggcg acgcccacgc cgaacggttc ggcaccgacg
     6061 gcggcaaggg cctgaccaac accgactacg cgagcatcgc cgagcagatg ggccgctcgg
     6121 cgctggcgcc cctggtgttc aactgcaacg cccccgacac cggcaacatg gaggtgctgc
     6181 tcaagtacgg caccccggag cagcaggagc agtggctcga cccgctgctc gacgggcgca
     6241 tccgcagcac cttcctcatg accgagcccg gcgtcgcgag ttccgatgcc acgaacatgc
     6301 aggccaccgc caccctcgac ggcgacgagg tcgtcctcga cggccgcaag tggtggtcca
     6361 ccggtgccgg tcaccccgac tgccgcatcg gcgtcttcat ggggctcacc gacccgagcg
     6421 cgcaccgcca ccgccagcac tcgatggtgc tcgtcccgct cgacgcgccc ggcgtcacgg
     6481 tcgagcgcat gctcaccgcg ctcgggcagt acgacgagcc cctcggtcac ggacaggtga
     6541 acctggatgc cgtccggttg cccgccagca acatcctcgc ggggccgggc cgcgccttcg
     6601 agatcgccca gggccgtctt ggcccggggc gcgtgcacca ctgcatgcgc ctcatcggcc
     6661 tcgccgagaa gtcgctcgag ctggcctgcg agcgctcgac ccagcgcatc gccttcggca
     6721 agccgatcgc caacctcggt ggcaaccgcg agcggctcgc ccgggcccgc atcgccatca
     6781 accaggcgcg gctgcacgtg ctgcacgccg cctggaagct cgacaccgtc ggcatcgagg
     6841 gcgcccagtc cgaggtctcc gagatcaagg cggccgtgcc ccagatggcc tgcgacgtca
     6901 tcgacctggc catccagctg cacggtggtg gcggcatgag cgatgacttc ccgctcgccg
     6961 cggcctacgc cggcgcccgc agccttcgcc tggccgacgg ccccgacgag gtgcacctgg
     7021 gtgtcgtcgc ccgtcacctg ctgcggccct acgccgagcg cgcggcggcg gccatcgcat
     7081 caaccgcaac ggcatccggt tcgacggccg gcggcagccg atgagccggc gcgtcctcgt
     7141 cacgggtggc gcctcggggc tgggcctggc cctgaccgag ctcatgctcg accggggaga
     7201 caccgtgctc gtcgtcgacc tcgccgagga ccggccggat gccgtgcccg agggtgccgc
     7261 ctaccgtcgc ctcgacgtgc gctcgcagca ggactgggat gccgcgatgg cgtgggtgcg
     7321 cgacacctgg ggcggcctcg acctgctcgt caacaacgca ggagtcgcga ccggcgggcg
     7381 catcgacgtc gagtcgatgt cggactggga gcgcgtcgtc gacatcaacc tgctcggggt
     7441 ggcccgcggg tgccacacct tcacgccgat gttcaaggcc cagaagtcgg ggcacatcgt
     7501 caacgtcgcc tcgctcgccg ggctcgtgca cgggccgggg atgtcgagct acaacgcgac
     7561 caaggcgggg gtcgtggccc tgagcgagac cttgtccttc gagctcgcgc cgtggtcgat
     7621 cgacgtctcg gtcgtctgcc cggcgttctt ccgcaccaac ctgcaccagt ccttctccgg
     7681 caaggacgcc gcgatgcagg aggcgggggt gcggctcatc acccaggcga gcgtggatgc
     7741 cgcgcacatc gccgcgatcg tgctcaaggg cgtcgacgcg cgcaagcggg tcatcctcac
     7801 cgaccggctc gggcggcagg cgtactactc caagcggttc gtgcgcccgc tctacgaccg
     7861 catgctcacc gcgcaggcca agcggttggc ccggcgcgcc ggcgccgacc ccgcggagct
     7921 cacccgtgac tgaggcactc gccgaggtgc gcgacgagga cgcgttcgac gtcgagcggg
     7981 tcgctgcctg gctgcgcgag aacgcctcgg atgccgtcga ccgggccgag ctcaccggcc
     8041 tgccggaggt gcgccagttc gtcggtgggg cctccaacct cacctacctg ctgcgtttcc
     8101 acagcggccg cgacctcatc ctgcgtcgcc cgccgttcgg caccaaggcc aagggcgcgc
     8161 acgacatggg ccgcgagcac gacctgcagg cggcgctggc cggcgcgttc ccgctggtgc
     8221 ccgcgatggt ggcgtcctgc aacgacgaga gcatcatcgg ctcgccgttc tacgtcatgg
     8281 agcgcatcga gggcacgatc ctgcggcgcg acatcccgcc cgagctgggg ctcgatgtcg
     8341 acggggtgcg ggccctgtgt gagaacgcga tcgacacgct ggtgaggctg cacgacgtgg
     8401 acgtcacggc atccgggctc gcggggttgg accgcggtga cggctacgtg gcccgccagg
     8461 tgcaggggtg gaccgggcgg ttccgacgcg cccacacccc cgatgtcggc gacttcgaga
     8521 gcacgatcgc gtggctggag gccaaccagc ccggggaccg gccgcacgtg gtgatccaca
     8581 acgacttccg gttcgacaac ctcgtgctcg acccgggtga cccgacccgc atcgtcgggg
     8641 tgctcgactg ggagctcgcg accgtcggtg acccactcat ggacctcggc tctgcgctgg
     8701 cctactgggt gcaggccgac gacgacccgg cgttcctcgc cgtgcggctc cagccgacgc
     8761 acacgcccgg catgctgacc cgcgccgagg tcgtgcagcg gtactgctcc gctcgctcgc
     8821 tcgacgtgac gccgcaggag tggctgttct acgacgtctt cggcacgttc cggctcgccg
     8881 gcatcgccca gcagatctac taccggtacc accacgggca gacgagcaac ccggcgtacg
     8941 cccgcttcgg ggacttcgtg cgcatcctcg acgcgcggtg cagtgccctg ctcgccgggc
     9001 agccctgacc ccttcctgac cctgacccgc cagacctgac ccgaaggatc acgacatgcc
     9061 caccaccctc atcaccggcg ccagttcggg actgggcgcc gagatggccc gccagctcgc
     9121 cgaccgcggc tgggacctcg cgctcgcggc ccgccgcacc gagcgcctcg aggagctgcg
     9181 cgacgagatc gtcgccaagc acccggggcg gcgcgtcgag ctgcgggcgc tcgacgtcac
     9241 cgacgacgac caggtgttcg aggtgttccg ggccttccgc gacgacctcg gggtcatcga
     9301 ccgcgtcgtc gtcaacgcgg gcctgggcaa gggtgccccg atcggcaccg gccgttacga
     9361 cgcgaaccgg cagacggcga tgacgaactt cgtcgccgcc ctggcccagt gcgaggccgc
     9421 gatggagatc tttcgtgacc agaagcgtgg tcacctcgtc atgatctcgt cggtgtcggc
     9481 gatgcgcggc atgcgcaaga ccgtcacgac ctacgccgcg accaaggccg gagtggccgc
     9541 gttggccgag ggcctgcgca acgagatgct cggtgagccc gacctcgaca tccgcgtctc
     9601 gacgatctac ccgggctaca tccgctcgga gatgaacgag cgggtcacaa agaccccgtt
     9661 catcgtcgac acccgcaccg gggtggccgc gatggtcgac gccatggaga aggagaaggc
     9721 caacgcgctg gtgccggcct ggccgtgggt gccgctcggc aaggcgatgc gtcacctgcc
     9781 cctgccgctg gtccggaaga tcatgtgacc atggggcgcg ttctcgtcac cgctcaggtc
     9841 gtgggggatg ccgtggcgcg ccttcgcgag gccggccacg acgtcgtctt ccgcgacatc
     9901 gacgccccga tgccccggga cgagttcctg gccgccctgc ccggggtcga cgctctggtc
     9961 gcgatgctcg tcgaccgggt ggacgctgag gtgttggatg ccgcggggcc ggggcttcgc
    10021 gtcgtcgcga acgtggccgt cgggtacgac aacgtcgacg tcgccgcctg tcgcgagcgg
    10081 ggggtgctcg tgacgaacac gccgggggtg ctgacggatg ccacggctga catcgccatg
    10141 tcgctgatcc tcatgtcgac acgggggctg ggcgctgccg agcggcgggt ccgtaccggg
    10201 acgccgtggc gatggagcct caccgctcac ctgggggtgg gcctgcgcgg gaagaccctg
    10261 ggcatcgtgg ggcccggggc gatcgggctg gcgacggctc gacgggcgaa ggcgttcgac
    10321 atgcgggtgc tgctgtcagg gcggtcgtcg ccggatcctg ccgtggtggg tgagctcgac
    10381 gcggcggtgc tcgacctcga cgagctgctc acggcatccg acgtcgtgtc gctgcactgc
    10441 ccgctcaccc cggccacgcg ccacctcatc ggggcgcgcg agctacggct gatgcggccg
    10501 ggcgcgcatt tggtcaacac gtcgagaggg ccggtcgtcg ccgaggctgc cctggtggcg
    10561 gcgctcgatg ccggtgagat cgcgggggcg gggctcgacg tcttcgagga cgagccgcgg
    10621 gtgcatcccg gcctgctcgg gcgtgaggac gtcgtgctgc tgccgcacgt gggctcggcg
    10681 acggtggaga cgcgcaccgc gatggctgac ctcgccgtgg caaacgtgct cgccgtgctg
    10741 gccggccggg acgcggtcag cccggtgggt ggctgagcca gtcgcgggcg atgcgctgac
    10801 cggtgtgccg cagcagggag ccctcatccg gtttggagga cgacgatgtg aaggatccag
    10861 atgcaatggc gaagcagcat tcgccatctt cgctcttcca gttgcagaag accttggatg
    10921 ccccatgctg agggcgctcg ccacaaatag tgggcaaaac tgaggggacg agaatgaaga
    10981 ttcgacgaat ttgtgcaggg gtggcgttgg cagccaccgt gattgcgccc caggctgcta
    11041 ccgccgcgcc gaccggtagc cccagcgcag cagtcgctgc gcagtcgagc attcaggctc
    11101 ggccttcgtc gcctgacatg acagtcgagc agtacatgga gatcttcaac gctgcagtgg
    11161 cagacggcgc ggttgcccgg ggagatggca cgatcgccta cgccctgcag aatggcagcg
    11221 aactcgtcat ggcggctccg cccacaaaga ccaagggcgg gtccccgcag gaccttggtg
    11281 cggggtggga cggctggagg ggaccataca tctcgttcaa ccgagtcgac cagagcgccc
    11341 tgctcgcggg cggagtagct gccttgtccg tggccgtgtg cgccataccg ggcgtgggtt
    11401 gggcctcttg cgctgccctt gtgggtatcg tcacgatcgc cgcgtactac gtcaatgagt
    11461 acggtcgctg ctccacctcg aagcccaatc tccgcgtgta cctcagcgga cgcagaggct
    11521 gttacgcgtg aatggctcat ctatcggcgg attcatcctc accattgcga tccccatcgt
    11581 tttcctcggc gcaagcacgc tcggatggcg acgcggagga tcgaggcaaa ggttgctgac
    11641 tttcaccctg ttgggtgtgg tcctcgcctg tcttgctttt gcgcttattg gtcggatcgt
    11701 cgatgtcgaa tcgttcacga tgacgtttgt cgtcgtgatg gtggggcttt ctgccggcac
    11761 ggtcatgatc gggaagaagc gcctgaatct ggcctagggt cagccacggg cctgatggac
    11821 tcggcacctg taggacgtgc cggcaggggc cgagtccatc gtcgcggacg gcgttcgcgc
    11881 cactcactcc gcgggcggct gcgtttcctc gtgcgcgatg cgctgacctg tgcgacgcag
    11941 cagagccgcg gcagcctcca tcgagcgcct cacgtcgggc tcgagctcgg tcagcgggta
    12001 tgcggcgcgc aggccgaggg ccgtcaggcc ggcgtcgtcg aggatgcgtc ggccgcacac
    12061 cgcgacgacg gggatgccgt gcgcggtcgc ccgtgtggcg acgcctcgca ctgccttgcc
    12121 cgtcagggtc tgggcgtcga gggacccttc gccggtgatg acgaggtcgg cccggctgag
    12181 ggctgcgtcg agcccgacga ggtcgaggac gacgtcgatg ccggagcggc ggtgcgcccc
    12241 gaggacggcc atgagcgcga agccgatgcc gcccgcggcg ccggcccccg gcatccggcc
    12301 ccccatcgac caatcggtca ctcggggtac gccgccttcc tgtccctgaa gcgacctatt
    12361 ggtcgatatc ggtacgtcgg ggtgtgactc gacgacgtcg gcccagtggg tgagcgctgc
    12421 ttcgaggagg tcgacctcgg caggggtggc gcccttctgc ggcgcgaaca ccgcagccgc
    12481 acctgtcggg ccgagcagtg ggctgtcgac gtcggcggcc agggcgatgt aggtgtgggc
    12541 caggcgcggg tcgagcccac tgaggtcgag gtgggccagc cgagccaacg cggccccgcc
    12601 gtcagggaga tcaaccccgc cggcatcgag cagtcgggca cccagggcag cgagcattcc
    12661 ggcgccgccg tctgtgctgg ccgagccacc gacgccgacg atgacctctg ccgccccgag
    12721 gtccagagcg gcgcgaatcg cctcgccgag accgcggctc gacgcggtga ggggtgccgg
    12781 aactgcgtcc ggcaaccgcc cgagcccgca gacatcagcc atctccacga gagcgacggt
    12841 gggggtcgct tcgggctgaa ggggaggggg atcaggggtg cggtggcgcg tatcggcagt
    12901 gctgcgctgt ggcgcagcag tgccgatacg tggaatcaga ccctgttcgg cgaccgggat
    12961 cggcgccaga tcctccctcc cgcctctcac ggctaccgtc gcggtcaccg acaagcccgt
    13021 cgggccgtgc actgccacgg gcacggcgcg ccacccagcc gccaccgcag cagcgacggt
    13081 gccctctcca ccgtcggcga tcggcacgac gacgacctca gcgtgaggcg cgacgtccag
    13141 caacccgagg cggacggcat cccccacctg caacgacgtc agagaaccct tgaacgagtc
    13201 gctggcgacg accactcgag gggcgcgcgc cggtcggccc acgtcagagg taccggtgca
    13261 tcacgtggag accgacacgg ccgtgggtgc gcgactcgaa ggcctcgggg accgtgccga
    13321 tgatccggaa cccgaggtcc ttccagaggg cgccagcagc ctcgttcgtc tcgaccacgg
    13381 cgttgaactg cattgcggca tagccctgtt cgtgggccca cgtgagcacc cactcgccga
    13441 gctggcgccc cactccgcgg ccgcgggccg ccggcgagac catgaaggat gccgtggcca
    13501 cgtggctccc gcgggctggc cggttgggcc ctgccttcgc cgatccgagg acggcccccg
    13561 actcgtcgac ggcgacgacg cacaccccgg gtggctcctc gacccacaga tcccacgcct
    13621 gctcgggcgt caggccgagc gggtaggcgt aggtctcacc agcctcgacg atgtcgtgga
    13681 agacggggaa gaacgccggc cagtcacctg gctcgatcga tcggatgacc accgcgatag
    13741 ctcagtcctc ctggggcctc atgtgcagcc acgggcgggt cagtccgaac ctgcgcccgg
    13801 tgatctcgtc gacctcgatg acgatcgtgt tgaacttcgc cgtgttgacc cacgggcgca
    13861 gcggcagcag ctcggtctcg gcctcttcgt ggtgctcgag ctggcgggcg cgaccccgga
    13921 tgacgacgct cgttgcctcg tcctcggtga actcgtcgat ctcgaaggcg acatcagcgt
    13981 tcatcgtcag cccgagcagc ttgctgccct cggcggtgcg gaacacgatt cgccggtcgg
    14041 cgtcgacggc gtagttgatc gggacgaggt ggacctcgtc ggccaggtgg aaggccagcc
    14101 gaccgaactc ctgcccgtgc agcatctccc agcaggcgtc atcggtgagg gtggtgacgg
    14161 gcgacggttc ggacatcgag cctcctgcgc tgggtttcgt gggtcaaatc tatcgggccg
    14221 tggcgaaggc ggctcgtcgg gggagaccgt tctcacccga accttcgtga cccgtcggta
    14281 cggtgcctgc atgagcccag cgcaggtcgt cgtcatcggt gccggacccg gcgggctggc
    14341 cgcggctgcc gcgctcggtg cgcgtggggt tcaggccctg gtcgtcgacc gcgacagcca
    14401 ggtcggcagc tcgtggcgca ggcactacga acggttgcac ctgcacacgc cgaggcggtg
    14461 gtcgggcctg ccgggctacc ggatcccacg ccggttcggc cggtgggtgg ctcgggnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgac accgcagcga acagcgcccg
    14641 gtggctggtc caccttgacg acggcccccc gctcgccgcc gagcacgtga tcgtcgccac
    14701 cggctacaac cacaccccgg tcacccccga ctggccgggc ctcgacggct tcaccggcga
    14761 cctcgtgctg gcccgcgact accgcaacgg tcgcccgtaa gccggccgcg acgtcctcgt
    14821 cgtcggcacc ggcaacacgg gcaccgagat cgcgaccgac ctcgccgagc acggggccac
    14881 ccgggtgtgg ctggccgtgc gcacgccgcc gcacatcatc cgtcgtgaca tgttgggctg
    14941 gcccgcgcag ggcaccggca tcatcgtgcg tcgcctgccg ccccggctcg tcgaccgggt
    15001 ggctcatggg ttggccgccg tccaggaacc cgacctcagc gcctacggga tgccgcgcgc
    15061 cgacccgggt ctgtactcgc gcgtgctcgt ggggcgggtg ccggtgcagg acgtcggcat
    15121 cgtcgatgcc atccgcacgc ggcgtgtcga acccgtggct gccgtcgagg cgtgctcgac
    15181 gggcgaggcc tgcccgtggt gcacggtgcc cacgagcccc cgggtgctcc ggggctgtgg
    15241 ttcaccggct tcacgaaccc gatctcgggg atgctgcgcg agctgcgcat cgacgccgag
    15301 cggatcgccg ccgccatcgc acgctgacac cgtgctcgcc cgccgtgcgg cggacccatc
    15361 aaccggcaat gcctcgatca gccgagtgcg cgagcacgga ccccgttcgc aggctcggcc
    15421 gaatgtcggc tttgccggtt ggcaggcccg gcgctgcgga ggcaaggggc tgagggggcg
    15481 gaggatgggg ggtgtctgca ggggtcgttg cccgccgcaa ggaattgctt cacgcgcgca
    15541 atggctcgtg gacgcccccc ttgggccggg ccacaccggg ccccgccgag ccccgccggg
    15601 ccccgccggc cggcttcagc cgggcaggcg aaccgtgtcg agcgtgaact tgtgctcacg
    15661 cagccgctcg accagcggca tcccgatcgc cgtcgcgggg gtgagcaccc cggcagcatc
    15721 gggcagcgcg tcaccgtcct gcagcagggc gagcgcggcc tggcccagca tcaccgcggt
    15781 gccgctgtag cccgggtcgt agggggccgc caccgtcgtt cggtaccgtg ccccgttggt
    15841 ggcctcgccg gtgacctcca tgcggaaccg tccggccgcc tgggcctgct cgctcgggcc
    15901 ctcgccgggc ttgggcagca cccggtccag cagcgaccga gtcggcccga acgacatccc
    15961 cgcgagccct gccccaaggg ccaccgacgt catccccgcg gcgaaggcgc cggtcgggcc
    16021 gctgccgtag tcggagtact cgacgtaccg gaacccctcg ccgtacccga gcagcgacgc
    16081 cgaccgggcg acgatccggg tgttgaaggc cgccatgacg aacgggccgg tgaagtggcc
    16141 gttgtcggca tcgcgcttga ccggcgacgc cttcgccagc ttcgcgagca ccgcggccgg
    16201 ccccgacgca ccggaagaac ccgacgaacc ggatgccgtg gcaccggatt cgctcgcgac
    16261 cttgcggtct cggccgggcc ggggaccctc ggcgagggcc cacgggtcgc cgacgatgcg
    16321 gcgcgtggtg gggtctgcct tgagctcgtc gacctgcacc cgggccgagt cgatggtgcc
    16381 gccactgaac ccgcccttca tgctccggac ggccaggtgg gtgcggccga ggtcagcacc
    16441 gtcggccttg gcggcctgcg ccgtgagcat caccccgagg tcgctcggga tcgagtcgaa
    16501 gccgcacgcg tgcacgatgc gcgccccggt gcgcttcgcg gtctcgtggt tggcggcgat
    16561 gctgcggtgc acgaagagca cctcaccggt gaggtcgcag tagtgggtgc ccgcctctgc
    16621 gcatgcggca gccaggggca cgccgtactt cacgtacggc ccgacggtcg tgacgacgac
    16681 gtgggcgcgg cgcgcgagat cggccacggc atcggggttc gtggcgtcga cgacgagcag
    16741 cggccacccg gccgcctggg acccgagccc gcgcgccagg tcctcgaggc gtgactgcga
    16801 gcgaccggcc agggcgatcc gcaccccctc gggggcctcg gcggcgaggt ggcgagcggt
    16861 caggcgcccc acgaatccgg tcgcgccgaa caggacgatg tcgaactctc gtgcactcgc
    16921 gctcatcccc ccaccctaaa gcgcccgtga gccgcggcat ccggcacagt gtcacccatg
    16981 cgcacgaacc cgcgggtcct ggccgtcgtc caggcgggtg gtcaggggtc tcggctcgac
    17041 gtcctgacgc gtgagcgggc caagccggcc ctgtcgttcg ccgggtcgtt ccagctgatc
    17101 gacgtcgccc tgaccaactg cgcccacagc ggcatctgcg acgtctggct gtcggtgcag
    17161 taccaggccg ggtcgctgca ccaccacctc gcgtccggtc ggccgtggga cctcgaccgc
    17221 acccgcgggg gcctgcgctg gctgatgccg cagcagggcg ggggatcggc ggcccaggac
    17281 ggcttctcca acggcaacgg cgacgacctg caccggttct ccgacgcgat ccgcgagttc
    17341 gccccggacg ccgtcgtcgt gatgagcacc gaccaggtgc tggcactcga cctgcgcccg
    17401 gtcgtggccg ctcacctgga gcgcggctcg cactgcacgg tcgtcacgac cgaggtgtcg
    17461 ctgacccagg cggccgacaa ggccgtcatc accatcggtc ggggctcgcg cgtcgcgcgg
    17521 ctggactaca agccggagcg cccgagcggc acgacgatcg cggcggaggt cttcgtctac
    17581 gacccgaccg tgctgctcgc cacccttgag gacctgcgcc gcgagttgtc gcacaccgat
    17641 gacggcaaca ccggtatcgg cgacttcggc gagcacctgc tgccgcgcat gatccgcgac
    17701 ggtgtcgtgc acgcctggcc gcacgagggg tactgggcag acctgggcac ccctgccgcg
    17761 tacctgtcgg cccaccgcga cctgctggcc gggcgcgtcg acgtgcttga ccgcccggac
    17821 tggccgctgc tcacccggtg gcccgaactg cccgcgtcac gggtcgacac gggtgccgtg
    17881 ctcgaggact tcgtgctgag cgccgggtgc cgcgtcaagg ggacggtgcg gcgcagcgtc
    17941 ctcgggcccg gcgtcgtggt cgagcccggc gcggtgatcg aggatgccgt gctgctggcc
    18001 ggcgttcgcg tctcgcgtga cgctcgggtc gtgaccgcga tcctcgacga gggcgtgcgg
    18061 gtcgaggtcg gggcacgcgt gggtcaggcg acccgggcca cgcgggccca cgaccaccac
    18121 atcgccgtgg tggggcgcga ctcggtcatc ggccgaggcg tcacggtgcc tgcgggtgcc
    18181 cggctcgagc cgggaacgac cgcgtgacag gggtcggtag gccgaacctg tgagcggggt
    18241 ggttggggtg ctgtgagaag tcgttgcagc cgtgaaggga ttgcttgcgc ggcgcaatga
    18301 gtgctggagg tggtgtacga cccctggctc acaggaagac cccgcagcaa gcgatcccga
    18361 aaccgccacc tttcggggtg gggcgcacca ccctgggagg gctcgccacc ctcacgggta
    18421 cgttgccgcc accgtcgcgg cgtagcggcc acgcacctcg gcggcccgat cgcgcacggc
    18481 ctcgtcgggc tcgaccgtga cgtcgacggc gacgtcccag accggaggcg cctcgccccc
    18541 ggccagagcc cacgcggcct gccgcgccgc accgaccgcg acgtactcac ccgcacccgg
    18601 cacagccacg ggcacgccga acacgtcggc cgccacctcc tgcacggcga ccgacgcggc
    18661 cgcaccgccg atgagcagca cccggtgcac ggggcacccg gctgcgcgca cggcatccac
    18721 cgccgccgcg agcccgagga gcatgccctc gacgacggcc cgcgcgaggt tctcgggcgt
    18781 cgcgttcgag cgggtcaggc cgtgcagcga ccccgtggcc gtcggcaggt tcggcgtgcg
    18841 ctctccgtcg aggtagggaa gcagcgtcag cccgccagca ccgggcgccg cagccagcgc
    18901 caaccggtcg agcccggcgt ggtcggtgtt cagcatggcg gcacccgccc cgagcacacg
    18961 ggcggcgttg agcgtgcaca ccaggggcag gaacccgccc tcggcgtcgg cgaacgactg
    19021 caccgagccg ctcgggtcgg cgctcgcgac gtcggtgcgg gcgaacgccg tgccactcgt
    19081 gccgagcgag acgacgacgt cgccgggtac caacccgagc cccagcgctg cgcccatgtt
    19141 gtcgcccgtc cccgctgcca cggcgaggcc ggatgccgtg tggccgacga cctcggacgg
    19201 ccccgccacc cgcggcacgg cgaggtcggc gccgcgcgtg gcgagggcga gcaggtcctc
    19261 gcggtacgac cgggtgcccg ccgaccagta gccggtgccg gaggcgtccc cggcatccgt
    19321 ggtccacgcc cgtggccccg acgacccctc gccgaggatg cgcccggtca gccagtcgtg
    19381 ggggagcacg cacgtggagg cccgggccag gttctcgggt tcgtggtcgg ccatccagcg
    19441 cagcttgctg accgtcgtgg ccgccacggg cacgaccccg gtggcgtcgg cccaggcctg
    19501 cgcgccgagc tcggccacga ggtcgtccgc cgaccccgcc gagcgggtgt cgttccacaa
    19561 cagcgccggc cgcaccacgg catccgactc gtccaacagc accatcccgt gctgctgccc
    19621 accgacggcg atcgcgctga cgccgtcgag gatgccgcca ccgctcgcct cctgccacgc
    19681 cgaccaccag tggcgcgggt cgacctcggt gccgtcgggg tggccggcgc gaccggtgcg
    19741 cacgacctca ccggtcaccg cgtcacagac caccaccttg cacgactggg tggaggagtc
    19801 gactccggcg acgagcgggc gatcggtctg gttgtccgtg ggcaccccgc gacggtagcg
    19861 cgtcagcggg ccgcgaactc cgctgcctga cgcgccgagt agacgcggtc aaccgcgtcg
    19921 atgctggccc ggctcgacag gtcgaccccg gccttcttcg cggccgcctt cgccgcgggg
    19981 gtgctcaggt cgggtgccag cacgagtgcc ggcttgatgg cgcgatcgga tgccgcgtag
    20041 cgcctctgct ggtcggccaa cctcttcgtg gagcccttgg ccgtggtgag cagggctgcc
    20101 gtggcggtga ccgtcgagcc ggtgaggtac gggctgaccg tgggggtgta gccgctcgcc
    20161 tggccggtgc ggttggggtg gtaggactcc tggatcgggt tggacagccc gttgatccac
    20221 tccgagctcg agcacaccgc gtggccgacg aacggggtcg tggggtcggc gaacgagaag
    20281 cccgccgcgg tggcacgagc gcgggtcgtg gagttgacca ggtcggccat ggcgttcagg
    20341 cgggtcatct cgctcgagga gaaccacgtg agcaggttgc agtcgacccc gttgaagatg
    20401 cgcgggtacc cgacgacggt gaccttggcc tggggagccc gcgccttgat gttggcgtag
    20461 agggtgttca gggcgccggg gagggtctgg ttcacgtagg cctgggcctt gtcgatggct
    20521 ccgttgcagt tgctcagcca cgccggctgg gcgcacgtgg tcagcacgct cgcgaagccg
    20581 gcgtcgttgc cgccgatcga gatgctcacg taggccgtgc tggagctcag ggcggagagc
    20641 tgggtgttcg agacgtcggc gatcttcgcg cccgagcagg cccggaagtt caggtcgtac
    20701 cccttggcgg aggcgatgag cgacgggtag gcgtacaccg agcgcaggca gctcgtgccg
    20761 tccgagatgt agctgcgggt gccggtgccc gaggagtagc tgtcgccgag cgcgacgtag
    20821 gacgggttcg cagcgtgggc cggtgcggcc gagaccacga gggccgcggt ggcggcgagg
    20881 gctgcggtgg acagggcacg tcgtcgggcc atggggttct cctcgaatca gcaggatcct
    20941 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgccgcg tagcatcgaa
    21061 cggtggccct gactgatctc tcgcgctgtt ccgtcgcctc gcgtcaccgt ggcgacccgc
    21121 tggcgggcag tgcccccgtc gcgtcgcgct ggctgctcat cgagcaccag ggcccgtggg
    21181 cgaagaagcc gctcgccacg cctccgctca cggcatccct cggtgccgag atcgaccgga
    21241 tctgcgcgtc gttcgggggc aaggtcctgc tcgtgcgccg ccccggccgc cggctgcccg
    21301 gtgaggaccc gcactcgtgg ttcgcgatcg acaccatcag gcgcacccgc gtcaggggct
    21361 cctggcggac cgccgacgac ctgcgcgagg cggcacatgc cctggggatg ccgttgtccg
    21421 acagcgacga ggacgccgac ccgatggtgc tcgtgtgcac ccacgccacc cgtgacgcct
    21481 gctgcgcggt gcgtggtcgg ccgatcgcgg cgaccttggc caaggccctg cccgaggagg
    21541 tctgggagtg cacccacctc ggtgggcacc ggttcgccgg cacgctgctg tcactgcccg
    21601 acggggcctg cttcggccag ctcgaccctg acaacgcgct ggccgccgtc atgggacatc
    21661 gcgccgggcg caccgatgct cgcttcctgc gcggcaccac ccgatgggac ccgcccgttc
    21721 aggcagccct ggccgcggtg ctcgccgagc acggcccggc agacctcgac gacctcgccc
    21781 ccggggccgc ggacaccgac ggggaccaca cgacggtcga ggtctacggc cgcggatcgc
    21841 tgccggctcg cgcggtcgtc gacgtcgtgc gcgaggccct gcccgacgcc ccgctcagct
    21901 gcggggacgt gcccaaggcc cacgtggccc accgcgcggt cctgcgcccc tgactcaggg
    21961 tgagggggcg gaggccggca gcaccgtggc cgtcgtgagc ggctcgtcgc gagagcacac
    22021 ccgctcgggg tccacctcgc gccggtcgat ccgcgacccg ttgacgagct gcaccccgtc
    22081 gtagctcggc cgcccctcga tgacgttgct gtaccggtag cgcgtgagcg tcgcgagtcg
    22141 gcagttcgtg ccgaaggtca gcacgtcgaa ggccgacggc tgcccgacga gctcctcccc
    22201 gggggcctgc tcgacgaagt ggttgaccgg cccaccgccg gtgaaggtgc cgacctgcac
    22261 ccggttgccc tcgaggtcgg tggagtgcat gtgcccgttg acgagcaccc cgccctcgac
    22321 tcccttgagc gcccacggtt cgtggctcac gaccacgtcg gatgccgtcc gcccaccgaa
    22381 cgcggcgacg aaggcttcgc gggcgggcac ctgcttcgcc ggcgagccgg tgcccgagtc
    22441 gccgaaccag cgcgggtcgt tgaaccccga gatcgtgacg cccccgatgc tggcctcggt
    22501 gtactcgccg ggcgtcggct cgagcagcac gacgttcggc acctgcgcga gccgggccag
    22561 gacggcggtg tcggtcgcgc tcgtcgcgtc gtggtttccc cggacgaaca ggtagggcac
    22621 ctcgagggac tcgatgccgc tgaagatgcc ggccgcctca ccctcggcaa ccgtcccgaa
    22681 gttgagcagg tcgccggcgt cgatgacgac gttcaccgac tcctcctgca cgatcgtgcg
    22741 catcaacgag tactggttgc cgccgtggat gtcgctgacg agcagggcgc gcagcgcggt
    22801 gtcggcctcg aggggtgccg ccgagtactt ctgctgcaac gccgtcgaca gggcgatgac
    22861 gttgcgcagg tacggcgcga cctgggtggc ccgggcctcg acgtcggaca ggatgccctg
    22921 gttgcgctgc agggtgccga ggatgccggt cgacgtgaag gtctcctggc gctgcggctg
    22981 gtaggtcgcg tacaacgagg ccgccgtcag cgcggtggcc agcacccacc ccgtgccgca
    23041 ggcgacgacg agggtgagcc gggggcgacg gcggcgcagc acggcatccg cccccaccac
    23101 cgcaccgatc agcacgagcc cgccgatggc gaagcgcagg aggacgtcga cggccacctc
    23161 gcggatggcc tcgctcagct cggccggacc gggtcgcagt gacgcgaggc tgacgttggg
    23221 gcgggacagc tcgtcggcga tgctcgcgtt cacctgtggc acggagcgga tgccgggggc
    23281 gaccccggag aagccgagtt cgaggtcacc gaacgccgtg gtcgcgagga tctggccgag
    23341 gtgggtcggg tcggggtcga gcgacacggt ggcctggtag ttgcgggtgt gggccgtcgc
    23401 gggggcaacc gtcgtcgcga tgacacccgc ggcgatgcac gccacggtca cggcgagcac
    23461 cccgaggccc tgaaggaccc gacgtgagcc gatccggtgg cctgcccacc accacacccc
    23521 gatcgccgtg acgagcggcc agatcaccgt cagggcggcg accacccgtt cgtggagccc
    23581 gaaggacgcg ctgctcagcc ccagcccgag gctcgcgacg gatgccgtga gcaccatcgc
    23641 cgccgtgcgc gcgacgcgct gggtgaggac tcgcgggccg ctctcggtgg cggcgaacca
    23701 cggccagagg ccgagcagga cgaaggatgc cgtggcgacc gccgtgtgcg cagcggagga
    23761 ctcggcgcgc gagggcagcg ggacggcggc gacgaacagg gtggcgacgc caccagcggc
    23821 caggatcagg cgcccggtgc gctgggctgc tccgagggcc caggccgtga cgacgtgggc
    23881 cagccccgtc accacgagcg ccgcggtcat cacccagcgg tgcggggtgg ccgaggcggc
    23941 gagggcgctg atcgtctcgg accgggcgtc gaaacccccg ggttgcagag cggcggccca
    24001 cgtccatccg ccgacgagcg ccacgacggc gaccgtcgca ctgagcacgg cggtcctggg
    24061 ccgccccctg cgtgcgggca tgc
//
LOCUS       DAOSOC010000022        23904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_2131_length_23904_cov_7.767957, whole genome shotgun sequence.
ACCESSION   DAOSOC010000022 DAOSOC010000000
VERSION     DAOSOC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 23904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23904
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2131_length_23904_cov_7.767957"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3738)
                     /gene="pglW"
                     /locus_tag="PL014_03325"
     CDS             complement(<1..3738)
                     /gene="pglW"
                     /locus_tag="PL014_03325"
                     /inference="COORDINATES: protein motif:HMM:NF033442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system serine/threonine kinase PglW"
                     /protein_id="HOT56153.1"
                     /translation="MAASGGERWVEVSPSQFTHETEGLALVRALLPDESPFRAWSNFE
                     FRDSRGKWHEVDLLVLGRGRLHLVELKYYSGTLRGDDHTWLRDGHRAETSPLKLARRK
                     AQYLATKLTDALHDWAKEKQVAVKDSRLIVPFVQESVFLHHPGLRCELSASSALGLYG
                     IDGASATSLLPGISDLLLEQPHKSAIGPNQEAILRELMARIGLVQRRERTAGSWTITE
                     QALADGEGWQEWLAHHSVAQRERARIRFQVTPAGAAASEEQRVRRVAEHEFRVMSRLQ
                     HDGILRPKDLVQSELGVGLAYDYDESWQRLDLWVAEQTHGMPLATQLSIVRQVGEALQ
                     YAHANKVVHRALTPRSVWVRPVPGTRGDVKVRVGGWEGAGILNPADLTRTAVPGVTAL
                     HDADGPAGNDTEEGGFGAPEGQWSPTADRARIDVFGLGALAFYVLSSRAPASGRAELT
                     QRLHTQDGLDLAVELPQVSTELRDLVLRATRPAPTKRTGDVSTFLALLDAAERAANRP
                     EESSADPLEATPGALLGGRFELVRRLGQGSTAVGLLVDDKEVPGAPSRVLKVALHDDA
                     GRRLADEAEVLRALDSPRIARLVEGPITVNGRQALLLESAGEQTLTHELQGRTRLSLD
                     LLERYGTDLLDAVVTLDRAGVDHRDIKPSNLGVREGRGDRTKHLVLFDFSLTRAAASA
                     TSAGTPPYLDPFLVGKRNAFDSAAERYAAAVVLFEMATGRAPLYGDDPDAAPATISDG
                     PRVTADLFDPTLAASLVAFFATALARDNGDRHQTAEAMRQAWREIFAAEATTEPDESA
                     DARAAAATLSTPLAESGLTARALSALEPERITTVGDLLATDPVRIARLKGVADATRKQ
                     VNRRIKEWRQRLGDVSAPTLAADRAAASDTLAAAAETLLAPVAGERTRSRAAMVRLVL
                     GIGTDLDAFATQAQLAAHLPDPVNAARAGQVLTELQQAWAADPAALALLTRLVEHVDH
                     TLTQAGLVLTPAEATARMAALFTPSAEEAAATGPDLRIAAGLVRLTLERIRELRRADE
                     GAAAPLVIRRRQGRVTLIGRDQSLLDVAEWVATKADELVARAGDPATAVVPARRAADE
                     LSLVFKAVPLPDGPPAPMAEPSRLVELAGALSPTTAAAANGDLHHRDLGVATALRLTF
                     GDVSSLQALSPKEVRDRVRVRFPALAPLPQRPRLDELIREAGLQLQFDDRAQGYRPLT
                     RTGDTTGLASRQPTTHATPLSGAGTSEVTRRVEESLRTRAFL"
     gene            3962..9895
                     /locus_tag="PL014_03330"
     CDS             3962..9895
                     /locus_tag="PL014_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HOT56154.1"
                     /translation="MAIEIDAIPVVTYAMAHNGIQVINAVRVVNRGDELRGATLRAEL
                     RDSQGRLSKPFTELLDLPVEAALVLRDLTLALDPAAMATVEAHRPGRLKVEILDEDDW
                     AVGSAEVDVIVHAAQHWSALPVGLGLEMLPAHVMPNSPEVAQVLRTASGILEARTGSP
                     SIEGYQADAERVDDIVRAVYEALQGLGIAYANPPASWHGRVEDAGQKVRTPREVVGER
                     VGTCLDLTVVMAACLEQAGIRPLLWVIEGHAFLGWWRDERALGSIVVTDASEVVNHLD
                     LGHMGLVETTALTKRTEPVDFDHATRIPRQQHLADLSVIEGVVDVRQARLGRVYPLPA
                     QGRAADGTPQIIEYSPAEHSTPVLIVTGGRAATRGAHQDSVPARVTTWKNSLLDLTLR
                     NRLLNYKESTGINLAAPGESLGQVEDLLHAGKAIHLLPSDQLDDVLRARGIRYGRELP
                     AEARAGLVATKQAVYTDVTSAAYLSRMRSLAYKAKTIVDETGANNLYLALGTLVWSVE
                     GRAEPLRSPLILVPVRLVTGARQSTYRLEIDETGQSTPNYCLLEKLKQVHGMSIPGLE
                     NPVADDAGIDLGAAFTAVRVAVQERGLSYHVEDTADIAILQFAKFRLWKDLDEHWETF
                     CDNALVSHLVHTPTAPFADSHDATGSPDLDALAALCPMPADGSQLRAVADAVSGRTFV
                     LEGPPGTGKSQTITNLLARAMAEGKRVLFVAEKRAALDVVKNRLDSVGMGPYSLDLHD
                     KGSKPAVVREQIRKAMDATPFADPQALKVAIEQHDSSAGRLSRYARRVHEENGAGHSL
                     YAATTDLLHRADTEYSFEVPRTLLTHEATTERESLRAMLRTLPDVAGPARPGPNNPWA
                     FVRVDELDAGPFAAVRQAAVEVDRAVERLAAVSALAGVLRTATRASELESLAALLRAP
                     SITLDTLDRTRSQSWRADSVAVRQEVHAFVAASHPGLDRATPAALELALPDIYGRAQA
                     AAASSWFGRKKRLRAVLAELQPGLRPGAVVQPKELVGLLAALVQLQGAVQFLAARTTA
                     VAGVRVPPNWNPLTPEGAGVVAQQLDWLEWAGAAVAPQDSGGTFQDELRRLLESGLTP
                     EAATIDAVAALAAALKQVFALTRATDDDVRQWLRGEGLVDRWVGTAALRSSLDDDLIS
                     LRRWLDLRRTVAALDRARMPAAAAALLDGSVEADDAPQAFEKGLAAASRAERLVSQNL
                     DAFDVRSHERSLRQYVDSGRQVRGLLQQALPREVIARRDFNAMSESGRIGGLRREVNK
                     TRRALPMRELMTQYGDLITQLMPCVLVSPDSLARFFPAESRMFDIVVFDEASQIRVAD
                     AIGAMGRASSVVVVGDSKQMPPSSFGEASANDDADSDVTASESLTDAESILSECLSAA
                     VPQQWLSWHYRSQDESLIAFSNLHYYEGRLSSFPAPGLGVGGGHSDRGLSLVRVGGVF
                     ERSGKGKTLRTNRKEAEAIVEEIRRRFEASPEAVPSIGVVTFNQQQRAFIEALIRDGG
                     DTRIIEALDSSQEEGLFIKNLENVQGDERDVILFSTAFSVNANGVLPLNFGPLSLEGG
                     ERRLNVAITRARRQVVLFTSFEPSQLRVEETKNVGVKHLRSYLEMADVGARALESARL
                     RRRDHDLHREDIASALTERGYVVETDVGLSDFRVDLTVALAAEPTRPVAAVLLDGPEW
                     SSRATIGDRDTLPVEVLGGMLKWPHVARIWLPAWLADREGVLDGFSESLDRALAGTPV
                     TVGVNGGASSGLHLPSQPVATAAAAMTVTAAPDAADAAPDDDVWSGVLGSSLQASLAG
                     PVGADGLRGGQAFRPWVVGLLGSRDTLDQLDRSAAARNRVGLAVADAIATEGPIHRDR
                     LVKLVAGAFDLARVNAKRADSILGVVPRGHRKGVEPAILWDLAVDPASWGGFRVDPAG
                     TRPVDHVPLREIANAMRAVCSDSGGAERGELHRDVLAIFGFKRLTQGIEVRMDEALDH
                     ALREALLERRGTSYVAGQR"
     gene            9906..10448
                     /locus_tag="PL014_03335"
     CDS             9906..10448
                     /locus_tag="PL014_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT56155.1"
                     /translation="MTVVVLAGGESRRFGSDKLAAPLAGTTVLDHLLGSLPLEWAVIA
                     VGEQRPTPRPLTWTREYPPGGGPLAGIAAGLALVTTDLTAVVAGDMPYAVPGLRTLAA
                     ALAAAGPDTAAAVAVDDEGHANPLLAVYRTTAARDIVPQPAHGIPAKTMLALPHLEVQ
                     ITGVTSRDVDTPEDLQSLQP"
     gene            complement(10507..11904)
                     /locus_tag="PL014_03340"
     CDS             complement(10507..11904)
                     /locus_tag="PL014_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT56156.1"
                     /translation="MSTAPAPARKGEWLESWDPENEQTWDKSLAWRTLWVTTFCLTLA
                     FVAWFLPSAIIPKLNALGYEFSKSQLYWMAAMPGLAAGLFRLVWMVLPPIMGTRKMVS
                     LTSLLLIASTLGWGVRVQTPEAPYWELMVLAFLAGIGGGAFSGFMPSTSYFFPKRMQG
                     TALGLQAGLGNFGVSFVQLLTPYFIAMGALAIFGGSQQMTIPGKEPATVWYQNAAFVW
                     IPLMVVGAILAWTMLKSVPVKANIRQQFDIFGNQDTWWMTLLYIMTFGTFSGLAAQFG
                     LLMTNLYGSGNTDIVQGSGASATLLIAGYAVPDAVKYVFLGPLIGAAARVAFSPLTDR
                     MGGAIWTLISGIGLIAAIAYTIPGLTPDTSSAATLESGFNQFLYGMLAIFLFAGIGNA
                     STFKQMPMIFERRQAGGVIGWTAAIAAFGPFFFGVGVTSLGPVAFYSIGIAWAVMCVA
                     ITWWRYARPGAPKPG"
     gene            complement(11915..13276)
                     /locus_tag="PL014_03345"
     CDS             complement(11915..13276)
                     /locus_tag="PL014_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT56157.1"
                     /translation="MTLTTEPDTAPAHPEVSSGAKRVLTMSTIAFTLMFAVWLMFGIL
                     GVPIQKELGLSDPELAWISAVAVLNGSLWRLPAGMLTDRVGGRVVTISMLLLTAIPCF
                     FLTQVSGYGALLVLAFLVGFAGNLFSVGIAWNAAWWPKERQGFALGVFGAGNVGASVT
                     KLLVVLLPTIIASTAGAMYLGIFPGGWRIFPAFYTVALVVVAILTFLIVPRHDKMNGA
                     GKPLGEMLAPLKHVRVWRFSLYYAAVFGAYVALSAWLPKYYVDNFGVELQVAALLTAT
                     FIFPASLLRPVGGWFSDKYGARRVMYWTFGVMLATTGILMMPNGHLVIDQADGTQSEH
                     LAYAIGIVPFALLVFLLGCAMGVGKAAVYKHIPEYFPDNVGSVGGLVGMFGGLGGFFL
                     PPLFAYTKVWSGFPSSTFFVLFLLTAACFVWMHWTVVHMLHRESPDLARHLETPVAPA
                     AVR"
     gene            13501..14286
                     /locus_tag="PL014_03350"
     CDS             13501..14286
                     /locus_tag="PL014_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT56158.1"
                     /translation="MSAPTDDLGPAAVLASPVRRRLLDTLAHATATSHDLTTPVGLTA
                     AELAEHVGLHLTTVRFHLDQLVAAGLVEASFHRSGSAGRPRKIYAPVQGSLAEVDVAG
                     EADALRLLSSLLAGAFADSSGGATPTPLEAGRRWAVEHVPADPASTPARTPGQWLSKV
                     GRMLDVLHEWGYTPEMSTSDGGRTARLVLKDCPFLALAVDNPAVVCGIHRGLITGSME
                     QFGEPDTEIGLEPFVGPATCVAHVSTRTPFRDKTPGTATKEPA"
     gene            14283..18020
                     /locus_tag="PL014_03355"
     CDS             14283..18020
                     /locus_tag="PL014_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HOT56159.1"
                     /translation="MTTSPALDGPLTNALVGTRRFFTRSQVSDDKRSIHTEGGRSGDA
                     FYRDRWSHDKVVRSTHGVNCTGSCSWKVYVKDGIITWEAQQTDYPSTGADRPEYEPRG
                     CPRGAAFSWYTYSPTRVRYPYVRGVLLEMFREAKAALGGDPVLAWASIVQDPDKARRY
                     KSARGKGGLVRSSWDEVVEIIAAAHVYTVKRWGPDRIAGFSPIPAMSPVSYASGARFL
                     ELVGAPMLSFYDWYADLPNASPQMFGDQTDVPESGDWWDAGYLIMWGSNVPMTRTPDA
                     HWMTEARYRGQKVVAVSPDYAENVKFADEWVAAAPGTDGALAMAMGHVILKEFFVDSQ
                     VDFFTQYNQQYTDLPFLVALEDAGDVADTAEGADGPAYRPGKYLVAGDLDIAQAASEN
                     AMWKPAVLDARTGDVAIPNGSIGFRYGDEGWGKWNLDLEDIDPVLTLHGLDAGGTASE
                     TARVVMPRFDAYDGKVAHVTRGVPVRRIGGRLVTTVLDLMLAQYGVARPGMPGTWPAD
                     YEDPSTPATPAWQEEITNVPAEQVVRLAREWAQNAIDTGGRGMILMGAGTNHWFHSDQ
                     IYRAMLVLTTITGCQGRNGGGWAHYVGQEKIRPIMGFQHMAFALDWQRPPRHMNQTAY
                     WYVNTSQYRYDTFTADDVDAGTGVFAGKGVMDLLAQSVRLGWTPSYPTFNRSSLALAD
                     EADAAGLAPADYVVDQLKSGALKFAVEDPEAEENHPRILSLWRANLLGSSAKGNEYFL
                     KHLLGTDNAVTAEQAPPDKRPTGIEWPEHVPEGKLDLLMTIDFRMTSSTLFSDVVLPA
                     ATWYEKHDLNTTDMHPFVHSFNPAISPPWQSKTDWEAWKAVAKKFSELAVDHLGTRRD
                     VIAKPLWHDTPEAMATVHGVVKDWRLGECEPVPGKTLPVLAVAERDYTAIYDKMTSIG
                     PLMEKVGMLTKGVPYDVKREVDILRHRNGVAHGGAGNGQPRLDTDIHMADAILHLSGT
                     TNGHLATHGFKNVEKRTGTQLHDLAAEHEGKQITFADTQVAPVPVITSPEWSGSESGG
                     RRYSPFTINIERKKPFHTLTGRQQFYLDHDWILGMGEALPVYRPPLNMTELFGEAALG
                     EQNALGVSVRYLTPHNKWSIHSEYQDNLFMLSLSRGGQTIWLSDVDAEKIGVADNDWI
                     EAVNRNGVVAARAIVSHRMPEGTVYMHHAQDRLIDVPLTETNGRRGGIHNSLTRIMMK
                     PSHMIGGYAQFAYFFNYIGPTGNNRDEVTMIRRRANQNVEY"
     gene            18022..19695
                     /gene="narH"
                     /locus_tag="PL014_03360"
     CDS             18022..19695
                     /gene="narH"
                     /locus_tag="PL014_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HOT56160.1"
                     /translation="MRVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRAGTEYVWFNNV
                     ETRPGLGYPRGYEDQEEWRGGWVMKNGRLQLRAGGRLKKLLTIFSNPKMPSISDYYEP
                     WTYDYETLLNSPAQKNFPVARPYSLISGKPVNVSWSANWDDDLGGSREHAAKDVMLKG
                     IEDKVKFEFEQTFMFYLPRICEHCLNPSCAASCPSGAIYKREEDGIVLVDQDRCRGWR
                     MCITGCPYKKVYFNHKTGKAEKCTFCYPRIEVGIPTVCSETCVGRLRYIGLVLYDADK
                     VLEAATTEDDTDLYESQLGVFLDPEDPEVRREAEAAGIPGDWIEAARRSPVKKLIMDY
                     KVALPLHPEYRTMPMVWYIPPLSPVVDVIKDTGYDAEDVDNLFGAIDTLRIPVEYLAN
                     LFTAGDVAPVNTVLKKLAAMRSYMRDINLGRDPRDEIPAAVGMEGEDMYEMFRLLALA
                     RYEERYVIPTAHAEQAHSLEELATECAVSEYGGGQEIFGEGSGSTPTPIAAENLRVRR
                     EALTADSIDELPPDNPLRGRVNLLNWDGRGMPDGIFPGGGSGSGERADR"
     gene            19692..20411
                     /gene="narJ"
                     /locus_tag="PL014_03365"
     CDS             19692..20411
                     /gene="narJ"
                     /locus_tag="PL014_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HOT56161.1"
                     /translation="MKWRRHRDARPGIREKDLVDTWQVVSLLLDYPDDVLLQRVPMLR
                     GVVEGLPDAQRMPLLEFLDHLTSARLGALQREYVDTFDVTRKCSLHLTYFTSGDTRRR
                     GVALVEFKQAYRRAGLEFDSEVELPDHLCVVLEFGAMQDWATAWHLLTRHRVGVEVLR
                     AGLAQRDSPWLPVLEALRSTLPPLEGSDAEALLRLVEQGPPQEEVGLEPYAIDPRLNP
                     RPEPADTTAMLGTTIPVGAPR"
     gene            20408..21157
                     /gene="narI"
                     /locus_tag="PL014_03370"
     CDS             20408..21157
                     /gene="narI"
                     /locus_tag="PL014_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOT56162.1"
                     /translation="MTTFLWVIVPYVCLAVFVVGHFWRYRYDKFGWTTRSSQLYEDRL
                     LRIGSPLFHFGMLGVVMGHVIGLIIPQVWTNALGLSPHTYHVIALAGGIPAGIAAVAG
                     LVILVYRRRTTGPVFSATTVNDKAMYAVLGTVILLGMWNTVAGSLLQFGGEYNYREGV
                     SVWFRSIFAFQPQPELMAAAPLGFQLHALLAFILFAMWPFTRLVHVFSAPLGYLTRPY
                     LVYRSKDERPGRGSGNRAPQRGWDKPELTRK"
     gene            21154..21426
                     /locus_tag="PL014_03375"
     CDS             21154..21426
                     /locus_tag="PL014_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HOT56163.1"
                     /translation="MTDLLAAEAQWHAWIADACTALDVDPDAVDVPGIHALTKDIAHG
                     FARPMAPVGSYILGLAVGARAARGEVVDPAALSEALRSTFPATSRA"
     gene            21553..21915
                     /locus_tag="PL014_03380"
     CDS             21553..21915
                     /locus_tag="PL014_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT56164.1"
                     /translation="MPDSTSRVDLGFAALADPVRREIIARLSRGPATVNEIAEPFDIS
                     LQAVSKHIQVLEHAGLVTRTRQAQRRPVHLNPSALEALTAWIDGYRLIHEQQFRRLDA
                     LLAADPESVTPSTPQEHS"
     gene            21912..22397
                     /locus_tag="PL014_03385"
     CDS             21912..22397
                     /locus_tag="PL014_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT56165.1"
                     /translation="MSNSLHLDVPAGVPWIDFTREFDAPVAAVFEAHRDPEKVKQWLG
                     PNGMQMDIAHWDFTTGGGYSYSHSNEHGTFGFRGVFHSIRENEFALQTFEFDGAPDEV
                     ALEFMWFEDLGDGRTRLRGRSIGRTVEGRDAMVESGMEHGMAQGYDRLEALAATDAAT
                     G"
     gene            complement(22442..22624)
                     /locus_tag="PL014_03390"
     CDS             complement(22442..22624)
                     /locus_tag="PL014_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56166.1"
                     /translation="MPASNRVWKIAGIAGLVGVVATGGLVARNERERRAYTPDEVRDR
                     LQQRLAETDKASRTDR"
     gene            22655..23362
                     /locus_tag="PL014_03395"
     CDS             22655..23362
                     /locus_tag="PL014_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56167.1"
                     /translation="MPITVPLPRLPRRETVPLDVAVEATRTGDLWLFRGGSVADRVIQ
                     TASNAPVNHVGMAVVLDDLPPLMWHAELGRSLRDHWTGDHHRGVQLHDLREAVTTWQV
                     KYGQGAWVRQLTPEVGREQEDAVLRTIARLNGVSFPTTAGMAWRWLRGRDGYLSKKAA
                     REKAVRPEAAYCAEVVGVTLEEMGILHDDVPMNWYDPGRFWSGDTLPLRAGWSYGVEV
                     GVEGPEEPAGPERDAPR"
     gene            complement(23293..23691)
                     /locus_tag="PL014_03400"
     CDS             complement(23293..23691)
                     /locus_tag="PL014_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56168.1"
                     /translation="MRRRTPRPVPDVFETFAHRVDDSAEAPLQAPDAGTVARSAGIGL
                     ALMVLTSVAFVLGAGVASRSRPDLVFSHLGWEGLAIVGWSLLWGALTAAVVLAISVVI
                     VGMPWVRRQRGASRSGPAGSSGPSTPTSTP"
     gene            complement(23710..>23904)
                     /locus_tag="PL014_03405"
     CDS             complement(23710..>23904)
                     /locus_tag="PL014_03405"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503071.1"
                     /note="broad specificity; family IV; in Corynebacterium
                     glutamicum this protein can use glutamate,
                     2-aminobutyrate, and aspartate as amino donors and
                     pyruvate as the acceptor; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOT56169.1"
                     /translation="EVYPIESDEAFVIDLLRAKKILVTHGTGFNWFEPDHFRLVTLPD
                     VDMLTEAIGRIADYLETIRN"
BASE COUNT         3687 a         8452 c         8409 g         3356 t
ORIGIN      
        1 aaggaacgcg cgggtgcgca acgactcctc cacgcggcgg gtcacctcgc tcgtcccggc
       61 accggacagg ggcgtcgcgt gcgtcgtcgg ctgccgggac gccaggcccg tcgtgtcgcc
      121 ggtgcgggtg agtgggcggt agccctgtgc ccggtcgtcg aactgcaact gcaggccggc
      181 ctcgcggatc agttcgtcga ggcgcgggcg ctgggggagc ggggcgaggg cggggaagcg
      241 cacccgcacc cggtcgcgga cctccttcgg cgacagggcc tgaaggctcg agacgtcgcc
      301 gaaggtgagc cgcagcgccg tggcgacgcc gaggtcgcgg tggtggaggt cgccgttggc
      361 cgcggccgcc gtcgtcgggc tcagggcccc ggcgagctcg acgagccggc tcggttcggc
      421 catcggtgcg ggcgggccgt cggggagggg cacggccttg aacaccagcg acagctcgtc
      481 agcggcccgg cgcgccggga cgaccgcggt ggcggggtca ccggcccggg cgaccaattc
      541 gtcggccttg gtggcgaccc actcggcgac gtcgaggagc gactggtcgc gcccgatgag
      601 ggtcacccgg ccctgacggc gccggatgac gagcggtgcg gcagcaccct cgtcggcccg
      661 ccgcagttcg cggatccgtt cgagggtgag gcgcaccagc ccggccgcga tgcgcaggtc
      721 cggcccggtc gcggcggcct cctccgcgct cggggtgaac agggcggcca tacgggccgt
      781 cgcctcggcc ggggtgagca ccaagcccgc ctgcgtgagg gtgtggtcga cgtgctcgac
      841 gaggcgggtg agcagggcaa gcgcggccgg atcggcggcc caggcctgct gcagttcggt
      901 gagcacctgg cccgcgcgcg ccgcgttgac cgggtcgggg aggtgggcgg ccaactgggc
      961 ctgggtcgcg aacgcgtcga ggtcggtgcc gatgccgagg acgagccgca ccatggcggc
     1021 ccggctgcgc gtgcgctcac cggccacggg ggcgagcagg gtctcggccg cggcggccaa
     1081 cgtgtccgat gccgccgcgc ggtcggccgc gagcgtcggg gcagagacgt cgccgagccg
     1141 ctggcgccac tccttgatcc gtcggttgac ctgcttgcgg gtggcgtcgg caacgccctt
     1201 gaggcgggcg atgcgcaccg ggtcggtcgc gagcagatca ccgacggtcg tgatgcgctc
     1261 gggctcgaga gccgacaggg cgcgcgcggt gaggccgctc tcggcgagcg gggtgctcag
     1321 cgtggcggcg gcggcgcggg cgtcggccga ctcatcgggc tcggtcgtcg cctcggcggc
     1381 gaagatctcg cgccaggcct gccgcatcgc ctcggcggtc tggtggcggt cgccgttgtc
     1441 gcgggccagg gccgtggcga agaacgccac gaggctggcg gccagggtgg ggtcgaacag
     1501 gtcggcggtg acccgggggc cgtcgctgat cgtcgcgggg gcggcatccg ggtcgtcgcc
     1561 gtacagcggc gcccggccgg tcgccatctc gaagaggacg accgccgcgg cgtagcgctc
     1621 agccgcggag tcgaaggcgt tgcgcttgcc cacgaggaag gggtcgaggt agggcggggt
     1681 gccagcgctc gtggcggagg cggcggcgcg ggtcagggag aagtcgaaga gcacgaggtg
     1741 cttggtgcgg tcgccgcgac cttcgcggac cccgaggttg gacggtttga tgtcgcggtg
     1801 gtccacgccc gcgcggtcga gggtcaccac ggcatccagg aggtcggtgc cgtaccgttc
     1861 gaggaggtcc agggagagcc gggtgcggcc ctggagctcg tgggtgaggg tctgctcccc
     1921 ggcgctctcg agcaggagcg cctggcggcc gttgaccgtg atcgggccct cgacgagccg
     1981 ggcgatccgc gggctgtcga gggcgcgcag cacctcggcc tcgtcggcca ggcgtctgcc
     2041 cgcgtcgtcg tgcagggcga ccttgagcac ccggctgggg gccccgggca cctccttgtc
     2101 gtcgacgagg aggccgacgg cggtcgaccc ctgaccgagc cggcggacga gctcgaaccg
     2161 gccgccgagg agggcgccgg gcgtggcctc gagggggtcg gcggagctct cctcgggccg
     2221 gttcgcggcc cgctcggcgg catcgagcag ggccaggaac gtgctgacgt cgccggtgcg
     2281 cttcgtcggg gccggccggg tggcgcgcag cacgaggtcg cgcagctcgg tcgagacctg
     2341 gggcagctcg acggcgaggt cgaggccgtc ctgggtgtgc aggcgctggg tgagctcggc
     2401 ccggccactc gccggtgccc tcgacgagag cacgtagaag gccagggcgc ccagaccgaa
     2461 gacgtcgatg cgggcgcggt ccgcggtggg gctccactgg ccctcaggcg cgccgaagcc
     2521 gccctcctcg gtgtcgttgc cggccggccc gtccgcgtcg tgcagagcgg tgacaccggg
     2581 gaccgcggtg cgggtcaggt cggccgggtt caggatgccg gcgccctccc acccgccgac
     2641 ccgcaccttg acgtcgccgc gagttcccgg caccgggcgc acccacaccg agcgcggcgt
     2701 gagtgcccgg tgcacgacct tgttcgcgtg cgcgtactgc agggcctcac ccacctgccg
     2761 gacgatcgac aactgcgtcg cgagcggcat cccatgggtc tgttcggcga cccacaggtc
     2821 gagccgctgc cacgactcgt cgtagtcgta ggccaggccg acgccgagct cggactgcac
     2881 gagatccttc gggcgcagga tgccgtcgtg ctgcaggcgg gacatcaccc ggaactcgtg
     2941 ctcggcgacc cggcggacgc gctgctcctc ggacgcggca gcgccggcgg gcgtcacctg
     3001 gaaccggatc cgggcccgct cccgctgcgc gacgctgtgg tgggccagcc actcctgcca
     3061 gccctcaccg tcggcgaggg cctgctcggt gatggtccac gagcccgcgg tgcgctcgcg
     3121 gcgctggacc aggccgatcc tggccatcag ctcgcgcagg atcgcctcct ggttcgggcc
     3181 gatcgcgctc ttgtggggct gctcgagcag caggtcggag atgccgggca ggaggctcgt
     3241 cgcgctggcg ccgtcgatgc cgtagaggcc gagggcgctg ctcgcggaca gctcgcagcg
     3301 cagcccgggg tggtgcagga acaccgactc ctggacgaac ggcacgatga ggcgcgagtc
     3361 cttgaccgcg acctgcttct ccttggccca gtcgtggagg gcgtcggtca gcttcgtggc
     3421 gaggtactgc gccttgcggc gggcgagctt gagcggtgag gtctcggctc ggtgcccgtc
     3481 gcgcagccac gtgtggtcgt cgccgcgcag cgtgccggag tagtacttca gctcgacgag
     3541 gtgcagccgt ccacggccga gcacgaggag gtcgacctcg tgccacttgc cgcgcgagtc
     3601 gcggaactcg aagttcgacc aggcccgaaa cggcgactca tcgggcagca gggcgcgcac
     3661 gagggccagg ccctcggtct cgtgggtgaa ctgggagggc gacacctcga cccagcgctc
     3721 gccccctgac gctgccacgc ccactccctc acgacccgag aatcccgggg acgcgtcccc
     3781 gccgactcgg gcattgtgtc gcaccctcgg cccgggatgg ccgggacccc cgaattgact
     3841 ctcgcgacgc gataccggtc tgcactcgtg tgcaccctgc ggcgccctgt gtcaccgcgg
     3901 ggccgtaggg tctcggcgac ggttcgtgaa cgaggggaca actgcagtgg atgctggcac
     3961 cgtggccatc gagatcgacg ccatcccggt cgtgacgtac gcgatggcgc acaacggcat
     4021 ccaggtcatc aacgccgtgc gggtggtgaa ccggggtgac gaactgcgtg gcgcgacgct
     4081 gcgcgccgaa ctgcgtgact cgcaggggcg gctgagcaag ccgttcaccg aactgctcga
     4141 cctcccggtc gaggccgccc tcgtgctgcg cgacctcacc cttgcgctcg atcccgccgc
     4201 gatggccacg gtcgaggcgc accggccggg gcgcctgaag gtcgagatcc tcgacgagga
     4261 cgactgggcc gtcggcagcg ccgaggtcga cgtcatcgtc cacgccgccc agcactggtc
     4321 ggccctaccc gtcggcctcg gcctggagat gctgccggcg cacgtgatgc cgaacagccc
     4381 cgaggtggcg caggtgctgc ggaccgcgag cggcatcctc gaggcccgca cgggtagccc
     4441 gtccatcgag ggctaccagg ccgacgccga gcgggtcgat gacatcgtgc gagccgtgta
     4501 cgaggccctt cagggcctgg gcatcgccta cgccaacccg ccggccagct ggcacggtcg
     4561 ggtggaggac gccggccaga aggtgcgcac cccccgcgag gtcgtcgggg agcgggtcgg
     4621 cacctgtctg gacctcacgg tggtcatggc agcctgcctg gagcaggccg gcatccgccc
     4681 gctgctctgg gtcatcgagg gccacgcgtt cctcgggtgg tggcgcgacg agcgcgcgct
     4741 cgggtcgatc gtcgtcaccg atgcgagcga ggtcgtgaac cacctcgacc tgggccacat
     4801 gggtctggtc gagacgacgg cgctgaccaa gcgcaccgag ccggtcgact tcgaccacgc
     4861 gacccgcatc ccccggcagc agcacctcgc cgacctgtcc gtgatcgagg gcgtcgtcga
     4921 cgtgcggcag gcccgcctcg gccgggtcta cccgttgccg gcacaggggc gcgccgccga
     4981 cggcaccccg cagatcatcg agtacagccc cgccgagcac agcacaccgg tgctcatcgt
     5041 caccggcggg cgggcggcga cccgtggagc gcaccaggac tcggtgcccg ctcgcgtgac
     5101 gacgtggaag aactcgctgc tcgacctgac cctgcgaaac cggctgctca actacaagga
     5161 gtccaccggc atcaacctcg cggcgccggg ggagtcactc ggccaggtcg aggacctgct
     5221 ccatgcgggc aaggccatcc acctcctgcc atccgaccag ctcgacgacg tcctgcgcgc
     5281 ccgcggcatc cgctacgggc gcgaactgcc cgcagaggca cgagcggggc tggtcgccac
     5341 caagcaggcc gtctacaccg acgtgacgag cgcggcctac ctgagcagga tgcgctcgct
     5401 cgcctacaag gccaagacga tcgtcgacga gaccggtgcc aacaacctct acctcgcgct
     5461 cggcaccctc gtctggtcgg tcgagggtcg ggcggaaccg ctgaggtcgc cgctcatcct
     5521 cgtgccggtg cggctggtga ccggtgcgcg gcagtccacc taccgcctcg agatcgacga
     5581 gaccgggcag agcaccccga actactgcct gctcgagaag ctcaagcagg tgcacgggat
     5641 gtcgatccca ggcctcgaga acccggtcgc cgacgacgcc ggcatcgacc ttggcgccgc
     5701 gttcaccgcg gtgcgggtgg cggtccagga acgcggcctg agctaccacg tggaggacac
     5761 cgcggacatc gcgatcctgc agttcgccaa gttccggctg tggaaggacc tcgacgagca
     5821 ctgggagacc ttctgcgaca acgcgctcgt ctcgcacctc gtccacacgc cgaccgcacc
     5881 cttcgccgac tcgcacgacg cgacgggtag ccccgacctc gacgcactcg ctgccttgtg
     5941 cccgatgccg gccgatggct cccagttgcg ggccgtcgcc gacgccgtgt ccggacgcac
     6001 cttcgtgctc gagggcccgc ccggcaccgg caagtcgcag accatcacca acctgctggc
     6061 ccgcgccatg gccgagggca agcgcgtgct cttcgtcgcc gagaagcggg ccgccctcga
     6121 cgtcgtcaag aaccgtctcg acagcgtggg catggggccc tactccctcg acctgcacga
     6181 caagggcagc aagcccgcgg tggtgcgcga gcagatccgc aaggcgatgg atgccacccc
     6241 cttcgccgac ccgcaggcgc tcaaggtggc gatcgagcag cacgactcca gcgccgggcg
     6301 gctctcccgc tacgcccgcc gggtccacga ggagaacggg gccgggcact cgctgtacgc
     6361 ggcgacgacc gatctgctgc accgggccga cacggagtac tccttcgagg tgccacggac
     6421 cctgctcacc cacgaggcca cgaccgagcg cgagagcctg cgggcgatgc tgcgcaccct
     6481 gcccgacgtg gctggcccgg ctcggccagg gccgaacaac ccgtgggcct tcgtgcgtgt
     6541 cgacgagttg gatgccgggc cgttcgcggc ggtgcgccag gccgctgtgg aggtcgaccg
     6601 ggctgtcgag cggctggccg cggtgtcagc gctcgccggg gtgctgcgca cggccacccg
     6661 agcctcggaa ctggagtcgc tggccgccct gctgcgggcc ccgagcatca ctctcgacac
     6721 tctcgaccgc acgcgcagtc agtcgtggcg tgccgacagc gttgcggtgc gccaagaggt
     6781 gcacgccttc gtggcggcct cccaccccgg gctcgaccgg gcgacccccg cggcgctcga
     6841 gctcgcgctg cccgacatct acgggcgggc ccaagccgcg gcggcctcga gctggttcgg
     6901 gcgcaagaag cggctgcggg ccgtgctcgc tgagctccag cccgggctgc gccctggagc
     6961 ggtcgtgcag cccaaggagc tcgtcgggct gctcgcggcc ctggtccagc tgcaaggtgc
     7021 cgtccagttc ctcgcggccc gcacaacggc agttgcgggg gtgcgggttc cgccgaactg
     7081 gaacccgctg accccggagg gtgccggcgt cgtggcccag caactcgact ggctagagtg
     7141 ggccggcgct gcggtggcgc cccaggactc ggggggtacc ttccaggacg agctgcgacg
     7201 gctcctggag tcggggttga cgcccgaggc cgccaccatc gatgccgttg ccgcgctcgc
     7261 ggccgcactc aagcaggtct tcgcgctcac tcgggccacg gacgacgacg tgcgccagtg
     7321 gttgcgtggg gagggcctgg tcgatcgttg ggtcggcacc gcggcccttc ggtcgagcct
     7381 cgacgacgac ctcatctcgt tgcgccggtg gctggacctg cgccgcaccg tggcggcgct
     7441 cgaccgggcc cgcatgcccg cggcagcggc tgcgctgctc gacggcagcg tcgaggccga
     7501 cgacgctccg caggcgttcg agaaggggct cgcagcggcg tctcgcgccg agcggctcgt
     7561 gagccagaac cttgacgcct tcgacgtccg cagccacgag cgcagcctgc gtcagtacgt
     7621 cgacagtggg cggcaggtgc gtggcctgct ccagcaggca ctgccgcgcg aggtgatcgc
     7681 ccggcgcgac ttcaacgcga tgagtgagtc gggccgcatc ggtgggctgc gccgcgaggt
     7741 gaacaagacc cgtcgggcgc ttcccatgcg cgagctcatg acgcagtacg gcgacctcat
     7801 cacccagctg atgccgtgtg tcctggtcag cccggactcg ttggcgcggt tcttcccggc
     7861 cgaatcgagg atgttcgaca tcgtcgtctt cgacgaggcg tcgcagatcc gggttgccga
     7921 cgccatcggc gccatgggtc gcgcatcgtc ggtcgtcgtc gtcggggaca gcaagcagat
     7981 gccgccgtcc tcgttcgggg aggcgagcgc caacgacgac gccgacagtg acgtgacggc
     8041 atccgagtcg ctcaccgatg ccgagagcat tctcagtgag tgtctgagcg ccgcggtccc
     8101 ccagcagtgg ctgtcctggc actaccgcag ccaggacgag tcgctcatcg ccttcagcaa
     8161 cctgcactac tacgagggtc ggctgtcctc gttcccggcg ccgggtctcg gcgtcggtgg
     8221 ggggcacagc gatcgcgggt tgagcctggt ccgggtggga ggtgtgttcg agcgctccgg
     8281 caagggcaag acgctgcgca ccaaccgcaa ggaggcggag gccatcgtcg aggagatccg
     8341 tcggcgcttc gaggcctcgc ccgaggccgt gccgtccatc ggtgtcgtca ccttcaacca
     8401 gcagcagcgg gcgttcatcg aggcgctcat ccgcgacggt ggtgacaccc ggatcatcga
     8461 ggcgctcgac tcctcccagg aggagggcct gttcatcaag aacctcgaga acgtgcaggg
     8521 cgacgagcgc gacgtcatcc tcttctcgac cgcgttcagc gtcaacgcca acggtgtgct
     8581 gccgctgaac ttcggcccgc tctccctcga gggcggtgag cgccgcctca acgtcgccat
     8641 cacccgtgcc cggcgccagg tggtgctgtt cacctcgttc gagccgtcgc aactgcgggt
     8701 cgaggagacg aagaacgtgg gggtgaagca cctgcggtcc tacctcgaga tggccgatgt
     8761 cggcgcccgg gcgctggagt ccgcccggct gcgacgacgt gaccacgacc tgcatcgcga
     8821 ggacatcgcc tcggcgctga cggagcgcgg ttacgtggtg gagaccgacg tgggcctgtc
     8881 ggacttccgg gtcgacctga ccgtggcact ggccgccgag cccacccgcc ccgtggcggc
     8941 cgtgctgctc gacgggccgg aatggagcag tcgggcgacg atcggcgacc gcgacacgct
     9001 gcccgtcgag gtgctcggcg gcatgctcaa gtggccgcac gtggcccgca tctggctgcc
     9061 cgcgtggctc gccgaccgcg agggcgtgct ggacggcttc tcggagtcgc tcgatcgcgc
     9121 cctggccggg accccggtga ccgtgggtgt caacgggggc gcctcgagcg ggctgcacct
     9181 accgagccag ccggtggcaa ctgcggcggc tgcgatgacc gtgacggctg cgccggatgc
     9241 tgcggatgcg gcacccgacg acgacgtgtg gagcggcgtg ctcggcagtt cgcttcaggc
     9301 gtcgctggcc ggcccggttg gcgccgacgg cctacgcggt ggtcaggcgt tcaggccgtg
     9361 ggtcgtcgga ctcctggggt cacgcgacac cctcgaccag ctcgaccgct cggccgcggc
     9421 ccgcaatcgg gtcgggctgg ccgtcgccga cgccatcgcc accgagggcc cgatccaccg
     9481 cgatcggctc gtcaagctcg tcgcgggcgc cttcgatctg gcgcgcgtca acgcgaagcg
     9541 cgcggactcg attctgggcg tcgtcccacg cggtcaccgc aagggtgtgg agccggcgat
     9601 cctgtgggac ctcgcggttg atccggcatc ctggggtggc ttccgcgtcg atcctgcggg
     9661 aacgcgaccg gttgatcacg tgccgttgcg cgagatcgcc aacgcgatgc gagcggtctg
     9721 cagcgactcg ggtggtgccg agcgtggcga gctgcaccgc gacgtgctcg cgatcttcgg
     9781 cttcaagcgc ctcacccaag gcatcgaggt tcggatggac gaggcgctcg accacgcgct
     9841 gcgcgaggcg ctcctcgagc gccgcggcac ctcctacgtc gccgggcaac gctgacgtga
     9901 ccccggtgac cgttgtcgtg ctcgccggcg gggagtcgcg ccggttcggc tccgacaagc
     9961 tcgccgcacc gctcgccgga accaccgtgc tcgaccacct gctcgggtca ctaccgctgg
    10021 agtgggccgt catcgccgtg ggggagcagc ggcccacccc gcgacccctc acctggaccc
    10081 gtgagtaccc acccggtggc ggcccgctcg ccggcatcgc cgccgggctc gccctcgtca
    10141 ccaccgacct gaccgccgtg gtggctgggg acatgccgta tgccgtgccg ggcctccgca
    10201 cgctggccgc ggctctcgcg gcggccgggc cagacaccgc tgcggcggtg gccgtcgacg
    10261 acgaggggca cgccaacccc ctgctggccg tctaccgcac caccgcggcg cgtgacatcg
    10321 tgccccagcc cgcgcacggc atcccggcga agaccatgct ggccctcccc cacctcgagg
    10381 tgcagatcac tggcgtcacg tcccgagacg tcgacacccc cgaggacctc cagtcccttc
    10441 agccgtgagt gcggaagtgg tggccagggc aaccacttcc gcactcacgg cgagggagag
    10501 ggccgatcag cccggcttgg gggcgcccgg gcgggcgtag cgccaccacg tgatcgcgac
    10561 gcacatgacg gcccacgcga tgccgatgga gtagaacgcg accgggccaa ggctcgtcac
    10621 gccgactccg aagaagaacg gcccgaacgc ggcgatggcg gccgtccagc cgatgacgcc
    10681 accggcctgc cgccgttcga agatcatcgg catctgcttg aaggtcgagg cgtttccgat
    10741 gccggcgaag aggaagatcg cgagcatgcc gtagaggaac tggttgaacc ccgactccag
    10801 ggtcgctgcc gagctcgtgt cgggcgtcag cccgggaatc gtgtacgcga tggcggcgat
    10861 gaggccgatg ccggagatga gcgtccagat cgcaccgccc atgcggtcgg tcagcgggga
    10921 gaaggcgacg cgggcagccg cgccgatgag cgggccgagg aagacgtact tcacggcatc
    10981 cggcacggcg tagccggcga tgagcagggt ggcgctcgcg ccgctgccct ggacgatgtc
    11041 ggtgttgccg ctgccgtaga ggttggtcat gagcagaccg aactgggcgg ccaggccgga
    11101 gaaggtgccg aaggtcatga tgtagagcag cgtcatccac caggtgtcct ggttgccgaa
    11161 gatgtcgaac tgctggcgga tgttcgcctt gacgggcacc gacttcagca tcgtccacgc
    11221 gaggatcgcg ccgacgacca tgagcgggat ccacacgaag gcggcgttct ggtaccagac
    11281 ggtcgccggc tccttgccgg gaatggtcat ctgctggctg ccgccgaaga tcgcgagggc
    11341 gcccatggcg atgaagtacg gggtgagcag ctgcacgaac gagaccccga agttgcccag
    11401 gcccgcctgc aacccgagtg ccgtgccctg catccgcttg gggaagaagt acgaggtgct
    11461 cggcatgaag ccggagaacg ccccaccgcc gatgccggcg aggaaggcga gcaccatgag
    11521 ctcccagtag ggggcctcgg gggtctgcac gcgcacgccc cagcccaacg tcgaggcgat
    11581 gagcagcagg ctggtcagcg acaccatctt gcgggtgccc atgatgggtg gcagcaccat
    11641 ccacacgagc cggaacagcc ccgcggccag acccggcatc gcggccatcc agtagagctg
    11701 gctcttgctg aactcgtagc cgagcgcgtt gagcttgggg atgatcgcgc tcggcaggaa
    11761 ccaggcgacg aaggccaggg tcaggcagaa ggtcgtgacc cacagggtgc gccaggccag
    11821 ggacttgtcc caggtctgct cgttctccgg gtcccaggac tccagccact cacccttgcg
    11881 ggccggtgcg ggggcggtgc tcatcgatgt ctccttagcg gacggccgcg ggagcgaccg
    11941 gggtctcgag gtggcgggcg aggtcgggcg actcgcggtg cagcatgtgg acgaccgtcc
    12001 agtgcatcca gacgaagcag gcggcggtca gcaggaacag gacgaagaac gtcgagctcg
    12061 ggaagcctga ccagaccttg gtgtaggcga acagcggcgg caggaagaat ccgccgagcc
    12121 cgccgaacat cccgacgagg ccgccgacgg agccgacgtt gtcggggaag tactcgggga
    12181 tgtgcttgta gacggcggcc ttgccgaccc ccatggcgca gccgaggagg aataccagca
    12241 gggcgaacgg gacgatgccg atggcgtagg ccaggtgctc gctctgggtg ccgtcggcct
    12301 ggtcgatgac gaggtggccg ttgggcatca tgaggatgcc ggtcgtcgcc agcatcaccc
    12361 cgaaggtcca gtacatgact cggcgggcac cgtacttgtc ggagaaccag ccaccgaccg
    12421 ggcgcagcag ggatgccggg aagatgaacg tcgcggtgag caaagcggcg acctggagct
    12481 cgacaccgaa gttgtcgacg tagtacttcg gcagccaggc cgacagcgcg acgtaggccc
    12541 cgaagaccgc cgcgtagtag aggctgaagc gccagacgcg cacgtgcttg agcggggcga
    12601 gcatctcgcc cagtggcttg ccggcaccgt tcatcttgtc gtgtcggggg acgatcagga
    12661 acgtcaggat ggcgacgacg accagcgcga cggtgtagaa cgcggggaag atccgccagc
    12721 cacccgggaa gatgccgagg tacatcgcgc cggccgtgct cgcgatgatc gtcggcagca
    12781 gcaccacgag cagcttggtc accgaggcgc cgacgttgcc ggcgccgaag acgccgaggg
    12841 cgaagccctg ccgctccttg ggccaccagg cggcgttcca cgcgatgccg acgctgaaca
    12901 ggttgcccgc gaagccgacg aggaaggcca gcacgagcag ggcgccgtag cccgagacct
    12961 gggtgaggaa gaagcacggg atggccgtga gcaggagcat ggagatggtc accacccggc
    13021 cgccgacccg gtcggtgagc atgccggcag gcaggcgcca cagtgacccg ttgagcacgg
    13081 cgacggcgga gatccacgcg agctcggggt cgctcagccc cagttccttc tggatcggca
    13141 cgccgaggat gccgaacatc agccacaccg cgaacatcag ggtgaaggcg atcgtcgaca
    13201 tcgtgaggac gcgtttggcg ccgctcgaca cctcgggatg ggcgggtgcc gtgtccggct
    13261 ccgtggtcag ggtcatgacc gtggcctcca tttggatgag agtcgtcatc taaatatctc
    13321 ggatcggacg gtacaaccgc cgttgaccag cctgccagtg aggtcaccct tatgtctacg
    13381 acggatcgtc gtacaccgaa aagtgagcct ctgtcaacgg aatctggtga cttcacgccc
    13441 gacgcgcgag tgtggttcga taggagaaat cctgtcctcc aaactgagga gcgacccgcg
    13501 ttgagcgccc cgacagacga cctcggcccc gccgccgtgc tcgcctcgcc cgtgcgccga
    13561 cgcctcctcg acaccttggc ccacgccacg gcgaccagtc acgacctgac cacccccgtg
    13621 ggcctgactg cggcagagct cgccgagcac gtcgggctcc acttgacgac ggtgcgcttc
    13681 cacctcgacc agctggtcgc cgccggtctg gtcgaggcca gcttccaccg cagcggcagc
    13741 gcgggtcgcc cgcgcaagat ctacgcgccg gtgcagggct cgctcgccga ggtcgacgtc
    13801 gcgggggagg ccgacgcgct gcgcctgctc agcagcctgc tcgcgggggc gttcgccgac
    13861 agcagcgggg gtgccacccc gacgccgctg gaggcgggcc gccggtgggc cgtcgagcac
    13921 gtcccggccg acccggcatc cacgcccgcg agaacgccgg gacagtggct gagcaaggtc
    13981 ggccggatgc tcgacgtcct gcacgagtgg ggctacaccc cggagatgtc gacctccgac
    14041 ggcgggcgca ccgcccgcct cgtgctcaag gactgcccgt tcctggccct ggccgtcgac
    14101 aacccggccg tggtctgcgg catccaccgc ggcctgatca ccggctcgat ggagcagttc
    14161 ggtgagccgg acaccgagat cggcctcgaa cccttcgtcg gccccgccac gtgtgtcgcg
    14221 cacgtgagta cccgaacccc cttccgtgac aagacccccg gcacggcaac caaggagccc
    14281 gcatgaccac cagccccgcc ctcgacgggc cgctgaccaa tgccctcgtc ggcacccgcc
    14341 ggttcttcac ccggtcacag gtgtccgacg acaagcgctc gatccacacc gagggcggcc
    14401 gctccggcga cgcgttctac cgcgaccgct ggagccacga caaggtcgtg cgcagcaccc
    14461 acggggtgaa ctgcaccggc tcctgctcgt ggaaggtcta cgtcaaggac gggatcatca
    14521 cctgggaggc gcagcagacc gactacccct ccaccggcgc cgaccgcccc gagtacgagc
    14581 cacgcggctg cccccgcggc gcggccttca gctggtacac ctactccccg acgcgcgtgc
    14641 gctacccgta cgtgcgcggt gtgctgctcg agatgttccg cgaggccaag gcggccctcg
    14701 gtggcgaccc cgtgctcgcc tgggcgtcca tcgtccagga ccctgacaag gcccggcgct
    14761 acaagtccgc ccgcggcaag ggcggcctgg tgcgttcgag ctgggacgag gtcgtcgaga
    14821 tcatcgccgc cgcgcacgtc tacaccgtca agcgctgggg cccggaccgc atcgccggct
    14881 tctcgccgat ccccgcgatg tcgccggtct cctacgcctc gggcgcccgc ttcctcgagc
    14941 tcgtcggcgc cccgatgctc tcgttctacg actggtacgc cgacctgccg aacgcgtcgc
    15001 cgcagatgtt cggcgaccag accgacgtcc ccgagtccgg cgactggtgg gatgccggct
    15061 acctcatcat gtggggctcg aacgtcccga tgacccgcac ccctgacgcg cactggatga
    15121 ccgaggcgcg ctaccgcggc cagaaggtcg tcgccgtctc gcccgactac gccgagaacg
    15181 tcaagttcgc cgacgagtgg gtcgccgcgg ccccgggcac cgacggcgcg ctcgcgatgg
    15241 cgatggggca cgtcatcctc aaggagttct tcgtcgacag ccaggtcgac ttcttcacgc
    15301 agtacaacca gcagtacacc gacctgccct tcctcgtcgc cctcgaggat gccggggacg
    15361 tcgccgacac tgccgagggc gccgacgggc cggcctaccg gcccggcaag tacctcgtcg
    15421 ccggtgacct cgacatcgcg caggccgcct ccgagaacgc gatgtggaag ccggccgtgc
    15481 tcgatgcgcg caccggtgac gtggccatcc cgaacggctc catcggcttc cgctacggag
    15541 acgagggctg gggcaagtgg aacctcgacc tcgaggacat cgaccccgtc ctgacgctgc
    15601 acggcctcga cgccggtggc acggcatccg agacggcccg ggtcgtgatg ccgaggttcg
    15661 acgcgtacga cggcaaggtc gcgcacgtga cgcgcggggt gccggtgcgc cgcatcggtg
    15721 ggcgcctggt gaccaccgtg ctcgacctca tgctcgccca gtacggcgtg gcccgccccg
    15781 ggatgccggg cacctggccg gccgactacg aggacccctc caccccggcg acgccggcgt
    15841 ggcaggagga gatcaccaac gtgcccgccg agcaggtcgt gcggctggcg cgtgagtggg
    15901 cgcagaacgc catcgacacc ggcggtcgcg ggatgatcct catgggggcc ggcaccaacc
    15961 actggttcca ctccgaccag atctaccggg cgatgctcgt gctcacgacg atcacggggt
    16021 gccaagggcg caacggtggc ggctgggcgc actacgtcgg ccaggagaag atccgcccga
    16081 tcatgggctt ccagcacatg gcgttcgcgc tggactggca gcgcccgccg cggcacatga
    16141 accagacggc gtactggtac gtcaacacca gccagtaccg ctacgacacc ttcaccgccg
    16201 acgacgtgga tgccggcacc ggcgtcttcg cgggcaaggg cgtcatggac ctgctcgccc
    16261 agtcggtgcg cctgggctgg accccgagct acccgacctt caaccgcagc agcctcgcgc
    16321 tggcggacga ggcggatgcc gcggggctgg ctcccgcgga ctacgtcgtc gaccagctga
    16381 agtccggtgc cctgaagttc gccgtcgagg acccggaggc ggaggagaac cacccccgca
    16441 tcctcagcct gtggcgggca aacctgctgg ggtccagcgc caagggcaac gagtacttcc
    16501 tcaagcacct gctcggcacc gacaacgccg tcaccgccga gcaggccccg ccggacaagc
    16561 gccccaccgg gatcgagtgg cccgagcacg tgcccgaggg caagctcgac ctgctcatga
    16621 ccatcgactt ccggatgacg agctcgacgc tgttctccga cgtcgtcctg ccggcagcca
    16681 cctggtacga gaagcacgac ctgaacacca ccgacatgca ccccttcgtg cactccttca
    16741 acccggcgat ctccccgccg tggcagtcca agaccgactg ggaggcgtgg aaggcggtcg
    16801 cgaagaagtt ctccgagctc gcggtcgacc acctgggcac ccggcgcgac gtcatcgcca
    16861 agccgctgtg gcacgacacc cccgaggcga tggccacggt gcacggtgtc gtcaaggact
    16921 ggcgcctcgg cgagtgcgaa cccgtgccgg gcaagaccct gccggtgctc gccgtcgccg
    16981 agcgtgacta cacggccatc tacgacaaga tgacctcgat cggcccgctc atggagaagg
    17041 tcggcatgct caccaagggt gtgccctacg acgtcaagcg cgaggtcgac atcctgcgcc
    17101 accgcaacgg ggtcgcccac ggcggcgccg gcaacggcca gccccggctc gacaccgaca
    17161 tccacatggc cgacgcgatc ctgcacctgt cgggcacgac caacggtcac ctcgccaccc
    17221 acggcttcaa gaacgtggag aagcgcaccg gcacccagct gcacgacctc gcggccgagc
    17281 acgagggcaa gcagatcacc ttcgccgaca cccaggtcgc gccggtgccg gtcatcacct
    17341 ccccggagtg gtccgggtcg gagagcggtg gccgccggta ctccccgttc acgatcaaca
    17401 tcgaacgcaa gaagccgttc cacacgctca ccgggcgcca gcagttctac ctcgaccacg
    17461 actggatcct cggcatgggg gaggcgttgc cggtctaccg gccgccgctg aacatgaccg
    17521 agctcttcgg tgaggccgcg ctcggcgagc agaacgccct cggcgtctcg gtgcgctacc
    17581 tgaccccgca caacaagtgg tcgatccaca gcgagtacca ggacaacctg ttcatgctca
    17641 gcctctcccg cggcgggcag acgatctggc tgtccgacgt cgacgccgag aagatcggcg
    17701 tcgccgacaa cgactggatc gaggcggtca accgcaacgg tgtcgtcgcg gcccgcgcga
    17761 tcgtctcgca ccggatgccg gagggcaccg tctacatgca ccacgcgcag gaccggctca
    17821 tcgacgtccc cctcaccgag accaacggga ggcgaggcgg catccacaac tccctcaccc
    17881 ggatcatgat gaaaccgagc cacatgatcg gcggctacgc ccagttcgcc tacttcttca
    17941 actacatcgg gcccaccgga aacaaccgcg acgaagtgac gatgatccgc cgccgcgcca
    18001 accagaacgt ggagtactga catgcgcgtc atggcccaga tggcgatggt catgaacctc
    18061 gacaagtgca tcgggtgcca cacctgttcg gtcacgtgca agcaggcgtg gaccaaccgg
    18121 gccggcaccg agtacgtgtg gttcaacaac gtcgagaccc ggcccggcct gggctacccg
    18181 cgcggctacg aggaccagga ggagtggcgc ggcggctggg tgatgaagaa cggccgcctc
    18241 cagctgcggg ccggcgggcg gctcaagaag ctgctgacga tcttcagcaa ccccaagatg
    18301 ccctcgatca gcgactacta cgagccgtgg acctacgact acgagacgct gctcaactct
    18361 cccgcgcaga agaacttccc cgtcgcccgg ccgtactcgc tcatctcggg caagccggtc
    18421 aacgtgtcgt ggagcgccaa ctgggacgac gacctcggcg gctcgcgcga gcacgcggcc
    18481 aaggacgtca tgctcaaggg catcgaggac aaggtgaagt tcgagttcga acagaccttc
    18541 atgttctacc tgccgcgcat ctgcgaacac tgcctcaacc cttcgtgcgc ggcgtcgtgc
    18601 cccagcggcg cgatctacaa gcgcgaggag gacggcatcg tcctggtcga ccaggaccgc
    18661 tgccgcggct ggcggatgtg catcaccggc tgcccgtaca agaaggtcta cttcaaccac
    18721 aagaccggca aggccgagaa gtgcaccttc tgctacccgc gcatcgaggt cggcatcccc
    18781 acggtgtgct ccgagacctg cgtgggccgg ctgcgctaca tcgggctcgt cctctacgac
    18841 gccgacaagg tgctcgaggc ggccaccacc gaggacgaca ccgacctcta cgagtcccag
    18901 ctgggcgtgt tcctggaccc cgaggacccg gaggtccggc gcgaggccga ggcggccggc
    18961 atccccggtg actggatcga ggcagcccgc cgcagcccgg tgaagaagct gatcatggac
    19021 tacaaggtcg cgctgccgct gcacccggag taccgcacga tgccgatggt ctggtacatc
    19081 ccgccgctgt cgccggtcgt cgacgtcatc aaggacaccg gctacgacgc cgaggacgtc
    19141 gacaacctct tcggggccat cgacaccctg cgcatccccg tggagtacct ggcgaacctg
    19201 ttcaccgccg gtgacgtcgc ccccgtcaac acggtgctca agaagctcgc ggcgatgcgg
    19261 tcgtacatgc gcgacatcaa cctcgggcgc gacccccgcg acgagatccc ggccgcggtc
    19321 gggatggagg gcgaggacat gtacgagatg ttccgcctgc tcgccctggc caggtacgag
    19381 gagcgctacg tcatcccgac ggcccacgcc gagcaggccc actcgctgga ggagctcgcc
    19441 accgagtgcg ccgtctccga gtacggcggc ggccaggaga tcttcggcga ggggtccgga
    19501 agcaccccga ccccgatcgc cgccgagaac ctgcgggtgc gccgtgaggc gctgaccgcc
    19561 gacagcatcg acgagctgcc gccggacaac cccctgcgtg gccgggtcaa cctcctgaac
    19621 tgggacgggc gggggatgcc ggacggcatc ttcccgggtg gcggctccgg ctcgggcgaa
    19681 cgggccgacc gatgaagtgg cggcggcacc gcgacgcgcg acccggcatc cgggagaagg
    19741 acctcgtcga cacctggcag gtggtctcgc tgctgctcga ctaccccgac gacgtgctgc
    19801 tgcagcgggt gccgatgctg cgtggcgtgg tcgagggcct gcccgacgcc cagcggatgc
    19861 cgttgctcga gttcctcgac cacctcacct ccgcgcggct gggcgccctc cagcgcgagt
    19921 acgtcgacac cttcgacgtc acccgcaagt gctcgctgca cctgacgtac ttcacctcgg
    19981 gtgacacgcg acggcgcggg gtggccctcg tggagttcaa gcaggcctac cgccgggccg
    20041 gtctggagtt cgacagcgag gtcgagctgc ccgaccacct gtgcgtcgtg ctcgagttcg
    20101 gcgcgatgca ggactgggcc accgcgtggc acctgctgac ccgccaccgg gtgggggtcg
    20161 aggtgctgcg ggccgggttg gcccagcgcg actcgccctg gctgcccgtg ctcgaggcgc
    20221 tgcgctcgac gctgccgccg ctggagggca gcgacgccga ggccctgttg cggctcgtcg
    20281 agcagggccc accccaggag gaggtcgggc tcgagccgta cgccatcgac ccccgactca
    20341 acccccgacc cgaacccgcc gacaccacag cgatgctcgg cacgaccatc ccggtaggag
    20401 ccccccgatg accacgttcc tgtgggtgat cgtcccgtac gtgtgcctgg ccgtgttcgt
    20461 cgtcgggcac ttctggcgct accgctacga caagttcggc tggaccacgc gctccagcca
    20521 gctctacgag gacaggctgc tgcgcatcgg cagcccgctg ttccacttcg gcatgctcgg
    20581 cgtggtcatg ggccacgtca tcggcctgat catcccgcag gtgtggacca acgcgttggg
    20641 gctgagcccg cacacgtacc acgtcatcgc cctcgccggt ggcatcccgg ccgggatcgc
    20701 cgcggtggcc ggactcgtca tcctcgtcta ccggcggcgc accaccggcc cggtgttcag
    20761 cgcgacgacc gtcaacgaca aggccatgta cgccgtgctc ggcaccgtca tcctcctcgg
    20821 catgtggaac accgtggccg gcagcctgtt gcagttcggc ggcgagtaca actaccgcga
    20881 gggcgtctcg gtgtggttcc gcagcatctt cgcgttccag ccgcagcccg agctcatggc
    20941 ggcggcaccg ctgggcttcc agctgcacgc cctgctcgcg ttcatcctgt tcgcgatgtg
    21001 gccgttcacc cggctcgtcc acgtcttctc ggcgccgctc ggctacctca cccggcccta
    21061 cctggtctac cgctccaagg acgagcggcc gggtcgcggg tcgggcaacc gggcgccgca
    21121 gcgtggctgg gacaagccgg agctgacccg caaatgaccg acctcctggc agccgaggcg
    21181 cagtggcacg cgtggatcgc cgatgcctgc accgcgctcg acgtcgaccc ggatgccgtc
    21241 gacgtgcccg gcatccacgc cctcaccaag gacatcgcgc acgggttcgc ccggccgatg
    21301 gctcccgtcg ggtcctacat cctcgggctc gcggtgggcg cccgggccgc caggggtgag
    21361 gtggtcgacc cggcggcgct gagtgaggcg ctgcgctcga ccttccccgc cacgtcccgc
    21421 gcctgattgg ccttcccgac ttcgtgcgac ttcggtgcgg gtttcgggcc gtccgcgctc
    21481 gaagccgcac gaaagtcgca cgaagtcgct atttaacttg acagttgatc aactgagtag
    21541 ttgaatattg ggatgccgga cagcacctct cgcgtcgacc tgggcttcgc ggccctcgcc
    21601 gaccccgtcc ggcgggagat catcgctcgc ctcagccgtg gcccggccac ggtgaacgag
    21661 atcgccgagc ccttcgacat cagcctgcag gccgtgtcga agcacatcca ggtcctcgag
    21721 cacgcgggcc tggtcacccg cacccggcag gcccagcgcc gccccgtgca cctcaacccc
    21781 tcggcgctcg aggcgctgac ggcctggatc gacggctacc ggctgatcca cgagcagcag
    21841 ttccgccgcc tcgacgcgct cctcgccgcc gaccccgagt cggtcacgcc cagcaccccc
    21901 caggagcact catgagcaac agcctgcacc tcgacgtccc ggccggtgtg ccgtggatcg
    21961 acttcacccg cgagttcgac gccccggttg cggccgtctt cgaggcccac cgcgaccccg
    22021 agaaggtcaa gcagtggctc ggcccgaacg gcatgcagat ggacatcgcc cactgggact
    22081 tcaccaccgg cggcggctac tcgtactccc acagcaatga gcacggcacg ttcggtttcc
    22141 gcggggtgtt ccacagcatc cgcgagaacg agttcgccct ccagaccttc gagttcgacg
    22201 gcgcccccga cgaggtcgcc ctggagttca tgtggttcga ggacctcggc gacggccgca
    22261 cccgcctgcg cggtcgcagc atcggccgca cggtcgaggg ccgcgacgcc atggtcgagt
    22321 cgggcatgga gcacggcatg gcgcagggtt acgaccggct cgaggccctc gcggcgaccg
    22381 acgccgcgac tggctgagcc gcgcccagag cggtggctga agtgcaatga gtgcggcagc
    22441 gtcagcggtc ggtgcgggac gccttgtcgg tctcggcgag gcgttgctga agccggtcgc
    22501 gcacctcgtc aggggtgtag gcccgacgct cacgctcatt gcgggcaacc agcccgccgg
    22561 ttgcgacgac accgacgagg ccggcgatcc cggcgatctt ccagacacgg ttcgaggcgg
    22621 gcatgggccc cagcgtattg ggcagactgt gcccatgccc atcaccgtgc ccctgccgag
    22681 gctgccccgc cgcgagaccg tccccctcga cgtcgcggtc gaggccaccc gcaccggcga
    22741 cctctggctg ttccgcggtg ggtcggtcgc cgaccgggtg atccagacgg cgagcaacgc
    22801 gccggtgaac cacgtcggca tggccgtcgt cctcgacgac ctgcccccgc tcatgtggca
    22861 cgccgagctc ggtcggtcac tgcgtgacca ctggaccggc gatcatcacc gcggggtgca
    22921 gctgcacgac ctgcgcgaag ccgtcaccac ctggcaggtc aagtacgggc agggggcctg
    22981 ggttcgccag ctgacgcccg aggtcgggcg cgagcaggag gatgccgtgc tgcgcacgat
    23041 cgcgcgcctg aacggcgtct cgttccccac cacggccggg atggcttggc gctggttgcg
    23101 aggccgcgac ggttacctgt ccaagaaggc ggcccgggag aaggccgtgc gccccgaggc
    23161 cgcctactgc gccgaggtcg tgggggtgac cttggaggag atgggcatcc tgcacgacga
    23221 cgtcccgatg aactggtacg accccggtcg gttctggagt ggtgacaccc tgcccctgag
    23281 agccgggtgg tcctacggcg tcgaggtcgg cgtcgagggg ccggaggagc cagccgggcc
    23341 cgagcgcgac gctccacgct gacgccgcac ccacggcatc ccgacgatga cgacgctgat
    23401 cgcgagcacc acggcggcgg tgagcgcacc ccagagcagc gaccagccga cgatggccag
    23461 accctcccag cccaggtgcg agaacacgag gtccgggcgc gagcgcgagg ccaccccagc
    23521 gcccaggacg aaggcgaccg aggtcagcac catgagcgcg aggccgatgc cggccgatcg
    23581 cgcgaccgtg ccggcatccg gggcctgcag aggcgcctcg gccgagtcgt ccacccggtg
    23641 ggcgaaggtc tcgaacacgt ccgggacggg acggggggtg cggcggcgca tcccccgagc
    23701 ataggggggt cagttccgga tggtctcgag gtagtcggcg atgcggccga tggcctcggt
    23761 gagcatgtcg acatcgggca gcgtgacgag ccggaagtgg tcgggctcga accagttgaa
    23821 gccggtgccg tgcgtcacga ggatcttctt ggcgcgcagc aggtcgatga cgaacgcctc
    23881 gtcgctctcg atcgggtaga cctc
//
LOCUS       DAOSOC010000023        22688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_2286_length_22688_cov_8.708435, whole genome shotgun sequence.
ACCESSION   DAOSOC010000023 DAOSOC010000000
VERSION     DAOSOC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 22688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22688
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_22688_cov_8.708435"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /locus_tag="PL014_03410"
     CDS             <1..1631
                     /locus_tag="PL014_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOT56170.1"
                     /translation="RKVYAAYKSATEHTGQPTVILAKTIKGYGLGTTFAGRNATHQMK
                     KFTLDDLKNLRDSLQIPISDEQLEADPYNAPYYHPGPDDDVIKYALERRAKLGGGVPE
                     RRVKHAPVHLPDDSAYSQVKKGSGKQQVATTMAFVRLLKDLMRDKEFGKRIVPIIPDE
                     ARTFGMDSFFSTIKIYNPHGQNYTPVDHDLMLAYREAKDGQILHLGINEAGSLAAFTA
                     AGSSYSTHGQPMIPIYVFYSMFGFQRVGDSIWAAADQMTRGFLIGATAGRTTLTGEGL
                     QHADGHSPLLASTNPAVVHYDPAFGFEIAHIMQDGLHRMFGTDDSLTDDERNVIYYLT
                     VYNEPMAQPVEPENVDREGILRGMYLYAAGSSEGLPESAPRVQLLASGVGMGWALEAQ
                     ELLRTDWGVVADVFSVTSWNELRRDGLRCDEAAFLTPDAEAPVPWVTQQLAGRPGPVV
                     AVSDYMRAVQDQIAQWVPGDFSSLGADGFGFSDTRPAARRFFHIDGPSMAVQALQALA
                     ARGEVDPSVAREAASRYRLNDVTAGASGNEGGDS"
     gene            1764..3191
                     /gene="argG"
                     /locus_tag="PL014_03415"
     CDS             1764..3191
                     /gene="argG"
                     /locus_tag="PL014_03415"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT56171.1"
                     /translation="MSKVLTSLPVGERVGIAFSGGLDTSVAVAWMREKGAIPCTYTAD
                     LGQYDEPEIDTVPDRAGQYGAELARLVDCKSALVEEGLSAIACGAFHIRSGGKTYFNT
                     TPLGRAVTGTLLVRAMHSDDVEIWGDGSTFKGNDIERFYRYGLLANPNLRIYKPWLDA
                     DFVAELGGRHEMSAWLTERNLPYRSSQEKAYSTDANIWGATHEAKTLEHLDESMEIVE
                     PIMGVKFWDPSVAIESEDVTITFVQGRPVAVNGRTLDPVALVDEVNTIGGRHGIGMSD
                     QIENRIIEAKSRGIYEAPGMALLHAAYERLLNAVHNEDTLANYYSQGRQLGRLLYEGR
                     WLDPQSLMMREAIQRWIASLVTGQVTLRLRRGEDYSIIDTRGDNFSYHPDKLSMERVE
                     NAAFGPLDRIGQLTMRNLDIADSRSKLEVYAGQPVEQGQLLVENGTLFGELPAGGYDQ
                     ITDNPLAPGSEDDALDRAAMEAGTD"
     gene            3243..4433
                     /locus_tag="PL014_03420"
     CDS             3243..4433
                     /locus_tag="PL014_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT56172.1"
                     /translation="MPSAPRPLPSAATRTRVARAAGDLGAAAVQQMEADHQWYRSLSA
                     EDRSWVGLVAQAGLSSFLEWFGGEQIRPAVTADVFGTAPRELTRSISLAQTLDLIRTV
                     IAVVEREIADLAAPGEEDLAREAVLRYSREVAFSAAEVYAQAAEARGAWDARLESLVV
                     DAVLRGEADESMQSRAAALGWGAVTQVAVVVGSTPPGATAPVLTDLHRAARRIDVELL
                     VAIHGPRLICIGGSVTDPIALAKALDPFLGDGPLVVGPTVPHLFAAGRSARAAISGHG
                     AAPAWVGAPRPVHADDLLAERALLGDLPARGALIARIIRPLAQSPGGTLLDTAAAYLD
                     GDLGVEGTARALIVHPNTVRYRLSGIAKAIGYDLTEAHDAQTVRIALAFHRLGPVSRS
                     TPRP"
     gene            4530..5465
                     /locus_tag="PL014_03425"
     CDS             4530..5465
                     /locus_tag="PL014_03425"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOT56173.1"
                     /translation="MIVIVCPGQGSQTPGFLSPWLELPGLRDQLSALSDAAQVDLVAH
                     GTTSDEETIKDTAVAQPLLVGAGLLALRALGGGTTVTDAVPQSGAIAGHSVGEITAAA
                     GAGVLSEKAAMEFVALRGRAMAQASAATPTGMSAVLGGDQGAVLATIGRHLLTPANVN
                     GAGQVVAAGSLPALAELAADPPARARVIPLKVAGAFHTHYMEPAVAVLRAAAADHEVA
                     DPEVTLVSNRDGQVVTDGRTVLDRLVAQVSNPVRWDLCMERFTALGVTAIIELPPAGT
                     LIGLAKRALKGVELLAVKTPDDLDAARTLIKEHSA"
     gene            5462..6481
                     /locus_tag="PL014_03430"
     CDS             5462..6481
                     /locus_tag="PL014_03430"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOT56174.1"
                     /translation="MSTTSKGFASALQSAPATGHARIAGIGGYRPRRVVPNSELVEAI
                     NSSDEWIRERSGIIERRFAGEGESVIDMSVMAAEPALEMAGLKGSDIDAILVATISWP
                     YQTPAAAPLIAERFGTDAMAIDLSAACAGYCHGIALANDMVRGGSAENVLVVGVERLS
                     DFTSRDDRGTAFIFGDGAGAAVISRSETPGIGPTIWGSDGEKWDAISQTDSWIDVREK
                     GLDWPHIGMQGQTVFRWAVWGMAPVAQRALDAAGVTVDQLDAFVPHQANVRIVEAMAK
                     KLNLPPDVAIARDIAYTGNTSAASVPLAMERMVRHGEIPRGGLALQIGFGAGLSYAAQ
                     VVVIP"
     gene            6563..6811
                     /locus_tag="PL014_03435"
     CDS             6563..6811
                     /locus_tag="PL014_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT56175.1"
                     /translation="MAQSEQEILAGLAEIVNEETGLDPAEVLADKSFTDDLDIDSLSM
                     MTIVVNAEEKFGVRIPDEEVKNLTTVGDAVSFIASNQA"
     gene            6951..8192
                     /gene="fabF"
                     /locus_tag="PL014_03440"
     CDS             6951..8192
                     /gene="fabF"
                     /locus_tag="PL014_03440"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOT56176.1"
                     /translation="MSERRVVVTGLGATTPLGGTIAETWDGILAGRSGAKPLDQEMLD
                     RYELPVHFACTIAQDPLEVLTKVQVRRMDPSAQYAVIASLAAMEDAGSPEIDPLRLGV
                     AIGTGIGGVWTLLDQWDNLREKGVRRVFPLSVPMLMPNTSAGNVSLLLGARAGAHTTV
                     SACASGAESMGTGYDMIRGGRADMVVAGGTECATHPICFAGFSQMQALSTRNDDPAAA
                     SRPYDTGRDGFVMGEGAAVMVLEEYEHAKARGARIYAEFASIGMSADAHHITAPEPEG
                     AGASRAMREAVERAGITDADVVHINAHATSTPVGDVAEANAIRRAFGAAADGMPVSAT
                     KSMTGHLLGAAGALEGLLTVLSVHHRVAPAAINIDDFDPAIDLDVVRGEHRALPQGDI
                     AALNNSFGFGGHNVALVIKSV"
     gene            complement(8277..8792)
                     /locus_tag="PL014_03445"
     CDS             complement(8277..8792)
                     /locus_tag="PL014_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HOT56177.1"
                     /translation="MSVSMPRTMTRGVVYVHSTPKALCPHILWAIEGVLGTRVTVDWT
                     DQPAEPGLVRAELSWSGQAGTGARIASALRGWDKLRYEVTEEPSQGCDGSRWSHTPRL
                     GIHHTWTSASGDAVINEDRLREVVVLAQGSPEAMQEMIEELLGTEWDDELEVFRYAGD
                     GAPVRWLHKVG"
     gene            9104..9180
                     /locus_tag="PL014_03450"
     tRNA            9104..9180
                     /locus_tag="PL014_03450"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9138..9140,aa:Ile,seq:cat)
     gene            complement(9250..10536)
                     /locus_tag="PL014_03455"
     CDS             complement(9250..10536)
                     /locus_tag="PL014_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT56178.1"
                     /translation="MTDVSTHTLQVPGAVLTYDIHQPAAPSGHRPLFIVGSPMAASGF
                     ENLVGHFTDRAVITYDPRGAERSTLATDGTVTGELHGEDMHRVVEASGLGPVDVFGSS
                     GGAAFALHWVVAFPGDVRTLVAHEPPLSTILEDSAMAARVTDDILATYQRDGHGPALA
                     KFIALVMHTGPLPDDYLDRPAPDPAMFGLPTEDDGSRDDPLLSRNLAIPPFLPDLDAL
                     RASTVRIVPAIGAEGEGTLARRGGEALARELGVEPVVFPGDHGGFTVSPMGPHNDPAA
                     FATRLREVLDVGDGARVIVTVGGSRTSSRAHLRAAGLGEDLQTIDLAQVVSKWIGETE
                     KNLNAVFDAAERSGAVLLLDEADALFGKRTEVRDSHDRYANLEVSDLLKRLEQHPGIP
                     ALVLRTSDRPLHVPKTVTVLDLEHRDGEQDGPVDGR"
     gene            10711..12117
                     /locus_tag="PL014_03460"
     CDS             10711..12117
                     /locus_tag="PL014_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOT56179.1"
                     /translation="MTGLRSMGAVDAIWLAMDSPDNLMVIDSVMMLEGPVDWERLASV
                     VQRRLVDRYPVFTQRVVEASTPLGMPHWENVEDFALAAHLHHVELPAPADDAAVQAFI
                     EARMAEPLPRDRPLWDFYLVDGHEDGAVVVARFHHALADGIALAQVLLSLTDETETGD
                     LEDAGQSPPVAASPPRHAGGLLDLPGRLTRDVASGVRSALHLFGEIPAALNPAHAIEA
                     MTVARQTAQIADKLLLSHEPQSPVSGDPGIAKRAVWSRPRSLVDVKLVGRATGATVND
                     ILIGAVSGAIATYVDTHGGDPEDLTTMVPVNLRDLREPLPRELGNKFALVMLPLPTGR
                     LAPLQRLAEAKSRMDTIKHSPEAVLTFGLINAIGRTNTEIAKQAIDFFAAKAIGVTTN
                     VAGPMTGRYLAGTRITSILGWVPGSGRHTLGVCIFSYDGVVRVGFKADAGVVTEPSGL
                     VDAFDEEMDTLVRLAAAV"
     gene            12145..12579
                     /locus_tag="PL014_03465"
     CDS             12145..12579
                     /locus_tag="PL014_03465"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT56180.1"
                     /translation="MPTDTASATVVVDAPLEEVLATIRDVDGIPGWVSDIIEAEVLTT
                     TEDGMPLTARFAASTPVGTDRYTLAYEHSPDGLSWHLVEGRLQTAQDATYTLVPVDDA
                     HTEVTFTLTISHPLPLPGFIRSRTIKGLVANNTGGLKQYLAP"
     assembly_gap    12686..12785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12887..14113)
                     /locus_tag="PL014_03470"
     CDS             complement(12887..14113)
                     /locus_tag="PL014_03470"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOT56181.1"
                     /translation="MNIAPDAEMSPAAGLPVELRGASAPTLMWAHEHEVEPAALNQLR
                     NIAALPWVHGVRVMPDVHLGKGATVGSVVAMRQAVSPAAVGVDIGCGMHAVKTSLTVE
                     DLPDDLGPLRSAIEAVIPTGFTAHDGSAPVLSTDRALSRTFGGLFARFGGLHAGVADR
                     EGRAAAQVGTLGGGNHFVELTADDHGAVWLMLHSGSRNIGKEIAERHINTAKGMEHNL
                     GLPDRDLAVFLDGTPEMAAYLRDLYWAQEYAMLNREAMMVTLRRVVSETFPHVTFADA
                     VRCHHNYVSQETYDGVDLVVTRKGAIFAGAGSLGLIPGSMGTGSYVVRGLGNTASFCS
                     ASHGAGRRMSRTAAKRHFTLEDLDAQTLGVECRKDAGVLDEIPGAYKDLEGVIAAQSD
                     LVEVVARLTTLLCVKG"
     gene            complement(14189..14264)
                     /locus_tag="PL014_03475"
     tRNA            complement(14189..14264)
                     /locus_tag="PL014_03475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14229..14231),aa:Arg,seq:acg)
     gene            complement(14643..16046)
                     /locus_tag="PL014_03480"
     CDS             complement(14643..16046)
                     /locus_tag="PL014_03480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT56182.1"
                     /translation="MTTDPAAVRASLADATPDVFWTDRPERPAPRAALTGSGHRADLV
                     IVGGGFTGLWAAVQAKEDNPSREVVLLERGRFGVAASGRNGGFVSPSLTHGLAQGLAC
                     WPEEIETLERLGAQNMTDLADSLTRHGIDADFHVPGEITMALTEHQVGTVREGYELHR
                     AHGIPVTLLDAAEARARVNSPRYLSGFLDPTVGLVDPARLVWGLADAAERLGVRLHED
                     TSVTGFDADIAGVTVRTEWGSVRADRVVLGTGAFRGPLKRLAHYTLPVYDHVLMTEPL
                     SPAQLDAVGWHGREGLTDAGNQFHYYRRTLDDRVLFGGYDANYHFPGRIDPRLEQSSS
                     HDLLARHFFEIFPQLQGLRFTHRWAGVIDTTTRFTPVFGTALGGRLAYAVGYTGLGVA
                     STRFGAQVALDLVDGRDTEVTALEMVRKKPLPFPPEPFRYVGVRATRASLAAEDRTGR
                     RNLWLRAMDAVGIGFDS"
     gene            16118..16453
                     /locus_tag="PL014_03485"
     CDS             16118..16453
                     /locus_tag="PL014_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT56183.1"
                     /translation="MPPRLLSPDVTTASLTHEALPADDVLHGSPTAAVLTLDALPTCE
                     VGLWEMTRGSARDTEVDEVFVVVSGRGTVTFADGERLDLAPGVAVRLRAGERTEWTTT
                     ETLRKVWIA"
     gene            16574..17347
                     /locus_tag="PL014_03490"
     CDS             16574..17347
                     /locus_tag="PL014_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOT56184.1"
                     /translation="MTEPQVVRDSAPKQPLVVMEGVNKHFGALHVLKDINLTVGKGEV
                     VILIGPSGSGKSTLCRTINRLETYESGSITIDGKKLPEEGKALADLRADVGMVFQSFN
                     LFAHKTILENVTLGPIKVRKMNKADADKRAMELLERVGIAHQAGKYPAQLSGGQQQRV
                     AIARSLAMDPKVMLFDEPTSALDPEMINEVLDVMVELANSGMTMVVVTHEMGFARKVA
                     DRVVFLADGQIVEMATPEEFFTNPKSERAKDFLGKILTH"
     gene            17398..18234
                     /locus_tag="PL014_03495"
     CDS             17398..18234
                     /locus_tag="PL014_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT56185.1"
                     /translation="MTTRRISMVAAAAVLALGLSACGSDDGGSDGGADGGSGLKIGIK
                     FDQPGLGLKVGDQYTGLDVDVAKYVAKELGTAEGDITFVQSPSAQRETLISSGQVDLI
                     FATYSITDARKEKISFGGPYFIAGQDLLVRADDTSITGPDTLDGKKLCSVTGSTSAQK
                     VKDKVAGVNLVEFGTYSECLAALLGGGVDALTTDDTILAGYAAQEANKGKLKVVGAPF
                     SEERYGVGLKKGDVALCEKVNAALKKMVEDGSWQKAVDANLGPAGYKAPAGNPPTPDA
                     CS"
     gene            18310..18972
                     /locus_tag="PL014_03500"
     CDS             18310..18972
                     /locus_tag="PL014_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOT56186.1"
                     /translation="MLETLSNYDVAAAVWVTIQLSVLSAIGALIIGTIVAVLRVSPVA
                     VLRGIGTSYVNIFRNTPLTVLLTLCVLGLSLLVGLQLSQDLSKDAFRWAVVGLSAYHA
                     AFVCEAIRSGVNTVPAGQAEAARSIGLTFTQSLREVLLPQAFRGSIAPLGSVLIALIK
                     NTTVAAVVGVADTAKLLATITENEGAQLGVFFVIAMIFVVLTLPIGVLATSLSQRMAV
                     KR"
     gene            18969..19970
                     /locus_tag="PL014_03505"
     CDS             18969..19970
                     /locus_tag="PL014_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOT56187.1"
                     /translation="MSTQNVLFDAPGPKARQRHAILTGVGVLLAGAVLYVILGRLADA
                     GQLDAAKWKPFVTDSSLWVDYLIPGLIGTLKAAILSVIFAGIFGLIFGMGRLSHIKAI
                     RYVSGAVVEFFRSVPVLMMMIFLYFGYFATSTIVPSSQGPLAAVVLALTLYNGSVIAE
                     LVRSGVHSLPKGQSEAGLSIGLTPSQTLRTIQLPQALTAMLPALISQFVVVLKDSALG
                     TAITYQELLTWSKTAGSAYANTVPALIVCAALFIAINYGLTRVATWVERRLNRRGTTA
                     GPVTTVAPTMIQGQGEPGGPAAHGTRIIGAGIESAEEAASDFVQSARERRKGHGDDT"
     gene            complement(20024..20096)
                     /locus_tag="PL014_03510"
     tRNA            complement(20024..20096)
                     /locus_tag="PL014_03510"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20061..20063),aa:Asn,seq:gtt)
     gene            complement(20175..20708)
                     /locus_tag="PL014_03515"
     CDS             complement(20175..20708)
                     /locus_tag="PL014_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56188.1"
                     /translation="MRLEWSGLRRPAVPAEVAEAIDPAGGEKLLAWAHDVGTGATVVA
                     GRHRLYAVAPDPEGARVTLSRPWHLVDAGSWGGEDGILKITWVDGQRPTRFQLTELGM
                     FPETLRERVQASVVLAETLDLGGRRTAKVVVRKDLATGALLSQAVLGPGVRSADPGVA
                     EQVSDGLRRVREQVGLD"
     gene            complement(20705..22642)
                     /gene="dnaG"
                     /locus_tag="PL014_03520"
     CDS             complement(20705..22642)
                     /gene="dnaG"
                     /locus_tag="PL014_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOT56189.1"
                     /translation="MAGRIRAEDIAAVKERTSIEDVIRDHVTLRPAGVGSLKGLCPFH
                     DEKSPSFTVRPAVGSYHCFGCGEGGDVISFVQKVEHLTFTESIERLASKIGFELRYEE
                     GGGPRDGGVSLGKRSRLIEAHRVAQEFYAEVLQDRTTAEARPGRDFLRERGFDGASAE
                     RFGIGFAPRSGEALTRHLRGRGFTEDEIVTGGLTGRGSRGLYDRFRGRLVWPIRDTTG
                     DTVGFGARRVLDDDRIAAKYLNTSETPIYKKSQVLYGLDAAKKAISTQRLAVVVEGYT
                     DVMAAHLSGVEGAVATCGTSFGIDHIKILRRIMRDETDGPQARVVFTFDGDAAGQKAA
                     MRAFAEDQRWAAQSFVAVAPDGMDPCELRLAKGDASVRALVDDAVPMFEFAVRTTIRR
                     FDLETAEGRVQAMKAVAPIVGSIRDTSLRPEYTRTVSGWLGIEVEQLAAEVKKAGRLK
                     HDDSADRQPRPASTDEVLEPGPFELAQLPAPDLRDPVVLTERQLLQALIQYPQQFTDA
                     DIDVLVPDAFTAPVHRAVFDGIRIAGHAARRASTMAWVTAVTDACALGVRGFVAQLSV
                     APLPTKFDPSTGLPPQRYLNSLVAGVRDAHLGRRIADAMASVRRLQNDPDADPADLQE
                     RSMALHRLELDRVALREANTS"
BASE COUNT         3496 a         8226 c         7501 g         3365 t
ORIGIN      
        1 accgcaaggt ctacgccgcc tacaagtcgg cgaccgagca caccgggcag cccaccgtca
       61 tcctcgccaa gacgatcaag ggctatggcc tcggaacgac cttcgccgga cgcaacgcga
      121 cgcaccagat gaagaagttc accctcgatg acctgaagaa cctgcgcgac agcctgcaga
      181 tcccgatcag cgacgagcag ctcgaggccg acccgtacaa cgcgccctac taccacccgg
      241 gcccggacga cgacgtcatc aagtacgccc tcgagcgccg cgccaagctc ggcggcgggg
      301 tgcccgagcg tcgcgtcaag cacgcccccg tgcacctgcc cgacgactcg gcctactccc
      361 aggtcaagaa gggctcgggc aagcagcagg tcgccacgac catggcgttc gtgcgactgc
      421 tcaaggacct catgcgcgac aaggagttcg gcaagcgcat cgtgccgatc atccccgacg
      481 aggcccgcac cttcgggatg gacagcttct tctcgaccat caagatctac aacccgcacg
      541 ggcagaacta cacccccgtc gaccacgacc tgatgctcgc ctaccgcgag gccaaggacg
      601 ggcagatcct gcacctgggc atcaacgaag ccgggtcgct ggccgcgttc accgccgccg
      661 ggtcgagcta ctccacccac ggccagccga tgatcccgat ctacgtcttc tactcgatgt
      721 tcgggttcca gcgcgtgggc gactcgatct gggcggccgc cgaccagatg acccgcggct
      781 tcctcatcgg cgccacggcg ggccgcacca ccctcaccgg tgagggcctg cagcacgccg
      841 acgggcattc gcccctgctc gcctcgacga acccggctgt cgtgcactac gacccggcgt
      901 tcggcttcga gatcgcgcac atcatgcagg acggtctgca ccgcatgttc ggcacggatg
      961 actcgctcac cgacgacgag cgcaacgtca tctactacct cacggtctac aacgagccga
     1021 tggcccagcc ggtcgagccc gagaacgtcg accgcgaagg catcctgcgc ggcatgtacc
     1081 tgtatgcggc cgggtcgagc gagggcctgc cggagtcggc gccgcgcgtg cagctgctcg
     1141 cgtcgggcgt cggcatgggc tgggccctgg aggcccagga gctgctgcgc accgactggg
     1201 gcgtcgtcgc cgacgtcttc tcggtcacct cgtggaacga gcttcgccgc gacggccttc
     1261 gctgtgacga ggccgcgttc ctcaccccgg atgccgaggc accggttccg tgggtcaccc
     1321 agcagctggc cggtcggccg ggccccgtgg tcgcggtctc cgactacatg cgggccgtcc
     1381 aggaccagat cgcccagtgg gttcccgggg acttctcctc gctcggcgcg gacgggttcg
     1441 gcttctccga cacccgcccg gcggcccgcc ggttcttcca catcgacggc ccgtcgatgg
     1501 cggtgcaggc cctccaagcg ctggcggccc gcggcgaggt cgacccgtcc gtggcccgcg
     1561 aggcggccag tcgctaccgc ctcaacgacg tgacggccgg cgcctccggc aacgagggcg
     1621 gcgacagctg agcgcctccg ctgcactctc cagtcgagag taggtgaatc gccgtctgac
     1681 ctccgggtcg gacggcgatt cccatactct cgacttcgac cccgagttcg tgaatatgca
     1741 gaggtctgta tagaattgca ctcatgtcca aggtcctgac ctccctgccc gtcggcgagc
     1801 gcgtaggcat cgccttctcc ggaggtctcg acacctccgt cgccgtcgcg tggatgcgcg
     1861 agaagggtgc gatcccctgc acgtacaccg ccgacctcgg ccagtacgac gagcccgaga
     1921 tcgacaccgt gcccgatcgc gccggccagt acggcgccga gctcgcccgc ctcgtcgact
     1981 gcaagagcgc gctcgtcgag gagggcctgt cggccatcgc ctgcggcgcg ttccacatcc
     2041 gcagtggcgg caagacctac ttcaacacca cccccctggg ccgcgcggtc accggcaccc
     2101 tgctcgtgcg ggcgatgcac tccgacgacg tcgagatctg gggcgacggc tcgacgttca
     2161 agggcaacga catcgagcgg ttctaccgct acggcctgct cgccaacccc aacctgcgca
     2221 tctacaagcc gtggctggat gccgacttcg tcgccgagct cggcggtcgc cacgagatga
     2281 gcgcctggct caccgagcgc aacctgccct accgctccag ccaggagaag gcctactcca
     2341 ccgacgccaa catctggggc gccacccacg aggcgaagac gctcgagcac ctcgacgagt
     2401 cgatggagat cgtcgagccg atcatgggcg tgaagttctg ggacccgagc gtcgccatcg
     2461 agtccgagga cgtgacgatc accttcgtcc agggtcgccc ggtcgccgtc aacggccgca
     2521 ccctcgaccc ggtcgccctt gtcgacgagg tcaacaccat cggcgggcgc cacggcatcg
     2581 gcatgtccga ccagatcgag aaccgcatca tcgaggcgaa gtcccgcggc atctacgagg
     2641 cccccggcat ggcgctgctg cacgccgcct acgagcgcct gctcaacgcc gtgcacaacg
     2701 aggacaccct ggccaactac tactcgcagg gccgccagct cggtcggttg ctctacgagg
     2761 gccgctggct cgacccgcag agcctgatga tgcgcgaagc catccagcgc tggatcgcct
     2821 ccctcgtcac cggtcaggtg accctgcggc tgcggcgcgg tgaggactac tccatcatcg
     2881 acacccgcgg cgacaacttc agctaccacc ccgacaagct gtcgatggag cgcgtggaga
     2941 acgccgcgtt cggtccactc gaccgcatcg gccagctgac catgcgcaac ctcgacatcg
     3001 ccgacagccg ctccaagctc gaggtctacg ccggtcagcc ggtcgagcag gggcagctcc
     3061 tcgtggagaa cggcaccctg ttcggcgagc tccccgcggg cggctacgac cagatcaccg
     3121 acaacccgct ggcccccggc tccgaggacg acgccctcga ccgggcggcg atggaggcgg
     3181 gcaccgactg accaccgcgg cgtccgggag gttgtgaacg gcatacaaat agactgattc
     3241 gtatgccgtc cgcccctcga ccgctgccgt cggccgccac ccgcacccgg gtcgctcgcg
     3301 ctgcgggcga cctcggtgcc gccgccgtgc agcagatgga ggccgaccac cagtggtacc
     3361 ggtcgctctc ggccgaggac cgctcgtggg tggggctcgt cgcccaggcg ggcctgtcct
     3421 cgttcctgga gtggttcggc ggtgagcaga tccggcccgc cgtcaccgcc gacgtgttcg
     3481 gcaccgcccc gcgcgagctg acccggtcga tctcgctcgc ccagaccctc gacctgatcc
     3541 gcacggtcat tgccgtcgtc gagcgggaga tcgccgacct cgcggcgccg ggcgaggagg
     3601 acctggcgcg tgaggcggtg ctgcgctaca gccgtgaggt cgccttctcc gctgcggagg
     3661 tgtacgcgca ggcggccgag gcacgcggcg cctgggacgc gcgcctggag tcgctcgtcg
     3721 tggatgccgt gttgcgcggg gaggctgatg agtcgatgca gtcccgcgcg gcggctctgg
     3781 gctggggcgc cgtgacgcag gtggccgtcg tggtcggctc cacgccgccc ggggccaccg
     3841 ctcccgtgct caccgacctg caccgcgccg cccggcgcat cgacgtcgag ctgctcgtcg
     3901 ccatccacgg cccccggttg atctgcatcg gcggcagcgt caccgacccg atcgcgctgg
     3961 ctaaggctct cgacccgttc ctcggcgacg gccccctcgt cgtcggcccg acggtgcccc
     4021 acctgttcgc ggcggggcgc agcgcccggg ccgccatctc cgggcacggt gccgcgcccg
     4081 cgtgggtggg tgccccgagg ccggtccatg ccgacgacct tctggccgag cgggccctgc
     4141 tcggtgacct accggcgcgc ggcgcgctga tcgcccgcat catccggccg ctcgcccaga
     4201 gccccggggg caccctgctc gacaccgcgg ccgcctacct cgacggcgac ctcggcgtcg
     4261 agggcaccgc gcgggccctc atcgtccacc cgaacaccgt gagataccgc ctgtccggca
     4321 tcgccaaggc gatcggttac gacctcaccg aggcgcacga cgcgcagacc gtgcgcatcg
     4381 cactggcctt ccaccgcctc ggcccggtga gccgaagcac accacgaccc tgactcgcgc
     4441 tcagtttgga ggaaacctcc aaaacagaga ccggaggttc gtcctcgttc gtcacggttt
     4501 ggtcacgacg caggcgacag gctggtcctg tgatcgtcat cgtctgccct ggccagggct
     4561 cccagacccc gggcttcctc tccccgtggc tcgagcttcc cggcctgcgt gaccagctgt
     4621 ccgccctctc ggatgccgcc caggtcgacc tcgtcgccca cggcaccacc tccgacgagg
     4681 agaccatcaa ggacaccgcc gtcgcccagc cgctgctcgt cggcgccggg ctgctcgccc
     4741 tgcgcgccct cggcggcggc accaccgtca cggatgccgt gccgcagtcg ggtgccatcg
     4801 cgggccactc ggtcggcgag atcaccgctg ctgccggcgc cggggtgctc tcggagaagg
     4861 ccgcgatgga attcgtcgcg ctgcgcggtc gagcgatggc gcaggccagc gccgcgaccc
     4921 cgacgggcat gagcgccgtg ctcggcggcg accagggcgc cgtgctggcc accatcggac
     4981 ggcacctgct caccccggcc aacgtcaacg gggccggtca ggtcgtggcc gccggctcgc
     5041 tgccggcgct ggccgaactc gccgccgatc ccccggcacg cgcacgcgtc atccccctga
     5101 aggtcgccgg cgcgttccac acgcactaca tggagcccgc cgtcgcggtg ctgcgtgccg
     5161 cggccgccga ccacgaggtg gccgaccccg aggtgacgct ggtgtccaac cgcgacggtc
     5221 aggtcgtcac cgacggccgc accgtcctcg accggctcgt cgcccaggtg tccaaccccg
     5281 tgcggtggga cctgtgcatg gagcggttca ccgccctcgg cgtcaccgcc atcatcgagc
     5341 tgccgccagc cggcacgctc atcgggctcg cgaagcgcgc cctgaagggt gtcgagctgc
     5401 tcgcggtgaa gacgcctgac gacctcgacg ccgcccgcac gctcatcaag gagcactccg
     5461 catgagcacc acctccaagg gtttcgcctc ggccctccag tcggccccgg ccaccggcca
     5521 cgcccgcatc gccggcatcg gcgggtaccg cccacgccgg gtggtcccga actccgagct
     5581 cgtcgaggcg atcaactcct ccgacgagtg gatccgggaa cgctcgggca tcatcgagcg
     5641 ccggttcgcc ggcgagggcg agtcggtcat cgacatgtcc gtcatggcgg ccgagccggc
     5701 tctggagatg gccggcctca agggttccga catcgacgcg atcctcgtcg ccacgatcag
     5761 ctggccctac cagaccccgg ccgcggctcc gctcatcgcc gagcggttcg gcaccgatgc
     5821 gatggccatc gacctctcgg ccgcctgcgc cgggtactgc cacggcatcg cgctggccaa
     5881 cgacatggtc cgcggcggct ctgccgagaa cgtgctcgtc gtcggggtcg agcgactgtc
     5941 cgacttcacc agccgcgacg accgcggcac ggcgttcatc ttcggtgacg gcgccggggc
     6001 cgctgtgatc agcaggtccg agaccccggg catcggcccg acgatctggg gctcggacgg
     6061 cgagaagtgg gacgccatct cccagaccga cagctggatc gacgttcgcg agaagggcct
     6121 ggactggccg cacatcggga tgcagggcca gaccgtcttc cggtgggcgg tgtggggcat
     6181 ggccccggtc gcccagcgcg ccctcgacgc cgccggtgtg accgtcgacc agctcgatgc
     6241 cttcgtgccg caccaggcca acgtgcgcat cgtcgaggcc atggccaaga agctcaacct
     6301 gccccccgac gtcgccatcg cccgcgacat cgcctacacc ggcaacacct cggcggcctc
     6361 ggtgccgctg gcgatggagc gcatggtccg ccacggcgag atcccccgcg gcgggctcgc
     6421 cctgcagatc ggcttcggcg cgggcctgtc ctacgccgcg caggtcgtcg tcatcccctg
     6481 atgagttccc gtgggcccac gggtcacccg ggccaagcac cacacccacc cacagcatga
     6541 ccatcgaaga aggagtaccg acatggcaca gagtgagcag gagatcctcg ccggactggc
     6601 cgagatcgtc aacgaggaga ccggcctcga cccggccgag gtcctcgcgg acaagtcgtt
     6661 caccgacgac ctcgacatcg actcgctgtc gatgatgacc atcgtcgtca acgccgagga
     6721 gaagttcggc gtgcgcatcc ccgacgaaga ggtcaagaac ctcaccaccg tcggtgacgc
     6781 cgtcagcttc atcgcctcca accaggcctg atgcaccacg gtccgtcacc gtcacccctg
     6841 ttcagcaggg tgacggtgac ggacactgga ccacacgtcc accgcacctc acacgccaca
     6901 ccaagccccc ggcatcccca cgtcccatcc cacaaggagc atcagcactc atgagcgagc
     6961 gacgcgtcgt cgtgaccggc ctcggtgcca ccacccctct cggcgggacc atcgccgaga
     7021 cgtgggacgg catcctcgca ggacggtccg gggccaagcc ccttgaccag gagatgctcg
     7081 accggtacga gctgccggtg cacttcgcgt gcaccatcgc ccaggacccg ctcgaggtgc
     7141 tcaccaaggt gcaggtgcgc cggatggacc cgagcgccca gtacgcggtc atcgcctcgc
     7201 tcgccgcgat ggaggatgcc gggtcacccg agatcgaccc gctgcgcctc ggcgtcgcga
     7261 tcggcaccgg gatcggcggg gtctggaccc tgctcgacca gtgggacaac ctgcgcgaga
     7321 agggcgttcg ccgcgtcttc ccgctgtcgg ttcccatgct catgcccaac acctccgccg
     7381 gcaacgtctc gttgctgctc ggcgcccggg ccggggcgca caccacggtc tcggcctgcg
     7441 cctccggtgc tgagtcgatg gggaccgggt acgacatgat ccggggcggc cgcgccgaca
     7501 tggtcgtcgc cggtggcacc gagtgcgcca cccaccccat ctgcttcgcc ggcttctcgc
     7561 agatgcaggc cctgtccacc cgcaacgacg accccgccgc cgcgtcgcgc ccctacgaca
     7621 ccggccgcga cggcttcgtc atgggtgagg gtgccgccgt catggtgctc gaggagtacg
     7681 agcacgcaaa ggcccgcggg gcacggatct acgccgagtt cgcctcgatc gggatgtccg
     7741 cggatgccca ccacatcacc gctcccgagc ccgagggcgc cggggcctcc cgcgccatgc
     7801 gcgaggcggt cgagcgcgcc ggcatcaccg acgccgacgt cgtgcacatc aacgcccacg
     7861 ccacctcgac cccggtcggt gacgtcgccg aggccaacgc gatccggcgc gccttcggcg
     7921 ccgccgctga cgggatgccg gtcagcgcca ccaagtcgat gaccgggcac ctgctcggcg
     7981 cggccggcgc gctcgagggc ctgctcacgg tgctctccgt gcaccaccgc gtggctcccg
     8041 cggcgatcaa catcgacgac ttcgacccgg ccatcgacct cgacgtcgtg cgcggcgaac
     8101 accgggcact cccccagggc gacatcgccg ccctcaacaa ctcgttcggc ttcggcggcc
     8161 acaacgtcgc gctcgtcatc aagagcgtct gaccggcaca gcaccggaca cgacgaagcc
     8221 ccccgcttcc tcttcggggg gcttcgtcgt gtgctgagcg aggttcctcg cccccctcag
     8281 ccgaccttgt gcagccagcg caccggtgcg ccgtcgccgg cgtagcggaa gacctcgagc
     8341 tcgtcgtccc actcggtgcc gagcagctcc tcgatcatct cctgcatcgc ctcagggctg
     8401 ccctgggcga gcacgacgac ctcgcgcagc cggtcctcgt tgatgacggc gtcgccgctg
     8461 gccgaggtcc acgtgtggtg gatgcccaac cgtggcgtgt gcgaccagcg ggagccgtcg
     8521 cagccctggc tcggctcctc ggtgacctcg tagcgcagct tgtcccagcc gcgcagcgcc
     8581 gaggcgatgc gggcaccggt tccggcctgc ccggaccagc tcagctcagc gcggacgaga
     8641 ccgggctcgg ccggttgatc ggtccagtcg acggtgaccc tcgttccgag gacgccttcg
     8701 atcgcccaca gaatgtgcgg gcacagagcc ttgggggtcg agtggacata gaccactccc
     8761 cgcgtcatgg tgcgcggcat cgagacagac atcctggcct ccttcgcgtg cgagggacgt
     8821 cttccccaac gccggtgcgc gcgatgtctg cctacggttc ctgccggtgt gccgtgcggg
     8881 ctctgctgtg tgggcacacg agcgtgccgt tatgggtcac atcatgcccc actgatccca
     8941 acggcaccag ccgtctcatg aatctcgtgc gtgacggcac gggtccttgg ttgacgagac
     9001 cggatgccgg tcactcacgg tacgcgagcg gcatccgtcg tcgttcctca cccaccgccc
     9061 cggtaggctc accgcgcccg ggacgcgaag gcgtcggggc agcggggcgt tagctcagtc
     9121 ggtcagagca gcggactcat aatccgtcgg tcgtcggttc aagcccgaca cgccccacca
     9181 cgtttcctca atggcgtggc ctggttgtcc tggttcgcgc ttcgacgtgc tcaccgcgac
     9241 cgcctcgcgt catctcccgt ccacgggacc gtcctgctcg ccgtcacgat gctcgagatc
     9301 gagcaccgtc accgtcttcg ggacgtgcaa gggacgatcg gacgtccgca ggacgagcgc
     9361 agggatgccc ggatgctgct ccagacgctt caacaggtcg gagacctcga ggttcgcgta
     9421 ccggtcgtgg gagtcgcgga cctcggttcg cttgccgaag agtgcgtcgg cctcgtcgag
     9481 gagcagcacc gctccgctgc gttcggccgc gtcgaacacg gcattcaggt tcttctcggt
     9541 ctcgccgatc cacttcgaga ccacctgcgc gaggtcgatc gtctgcagat cctcgccgag
     9601 tcccgccgcc cgcaggtgcg ccctcgaaga cgtccgggac ccaccgacgg tcacgatcac
     9661 gcgggcacca tccccgacat ccagcacctc acggagcctg gtggcgaacg ccgcgggatc
     9721 gttgtgcggc cccatcgggc tgacggtgaa cccaccgtgg tcccccggaa acacgacggg
     9781 ctccaccccg agctccctgg ccagtgcttc tccgccgcgg cgggcgagag tgccctcccc
     9841 ctcggcaccg atcgcgggaa cgatgcgcac cgtcgatgct cgcagtgcat cgaggtcggg
     9901 caggaagggc gggatggcca ggttgcggct gagcagcggg tcgtcgcgcg agccgtcgtc
     9961 ctcggtgggg agaccgaaca tggcggggtc cggcgccggc cgatcgaggt agtcgtcggg
    10021 gagcgggccc gtgtgcatga cgagcgcgat gaacttcgcc agtgccgggc cgtgcccgtc
    10081 acgctggtag gtcgcgagga tgtcgtcggt gactcgggca gccatggccg agtcctcgag
    10141 gatcgtggac agcggaggtt cgtgcgcgac cagcgtgcgg acgtcaccgg ggaaggcgac
    10201 cacccagtgc agggcgaagg cggctccgcc actggacccg aacacgtcga ccgggcccag
    10261 gccgctggcc tcgacgaccc ggtgcatgtc ctcaccgtgg agctcgccgg tgacggtgcc
    10321 gtccgtggcc agcgtgctgc gctcggcgcc gcgggggtca taggtgatga ccgcgcggtc
    10381 ggtgaagtgg cccacgaggt tttcgaagcc ggatgccgcc atcggcgaac cgacgatgaa
    10441 cagcggacgg tgcccgctcg gcgccgctgg ctgatggatg tcgtaggtca ggaccgctcc
    10501 gggaacctgg agtgtgtgag tgctgacgtc ggtcatcagg aacgtccttc cacggtgctc
    10561 gaccccggcc gggtgaggga gtcaaggtag cgacaagtcc gtgcgcggtc caccgtctgt
    10621 gcctcttccc ggggggctga cgagcagttc gacgtgtgat ctgcgttggg tcgtgcgtgt
    10681 cggccctcgg gacgggagga caatggagcc atgaccggac tccgatcgat gggtgccgtc
    10741 gacgccatct ggctcgccat ggacagcccg gacaacctca tggtgatcga ctcggtgatg
    10801 atgctcgagg gaccggtcga ctgggagcgc ctggcgtccg tcgtccagcg tcggctggtc
    10861 gaccggtacc cggtgttcac gcagcgggtc gtcgaggcgt cgacgcccct cggaatgccc
    10921 cactgggaga acgtggagga cttcgccctc gctgcgcacc tgcaccacgt cgagctgccc
    10981 gcacccgccg acgacgccgc cgtgcaggcg ttcatcgagg cgaggatggc cgagccgctg
    11041 cctcgcgacc ggcccctgtg ggacttctac ctcgtcgacg ggcacgagga cggtgccgtc
    11101 gtcgtggccc gcttccacca cgcgctggcc gacggcatcg ccctggccca ggtgctgctg
    11161 tccctcaccg acgagaccga gaccggggac ctcgaggatg ccgggcagtc accaccggtc
    11221 gcggcctcgc cgccgcgcca cgcgggtggg ctgctcgacc tacccggccg tctgacgcgc
    11281 gacgtcgcct cgggggtgcg ctcggccttg cacctgttcg gcgagatccc cgcggccctc
    11341 aacccggcac acgccatcga ggccatgacg gtcgcgcgac agaccgcgca gatcgccgac
    11401 aagctcctcc tctcgcacga gccgcagtcc cccgtcagcg gcgacccggg catcgccaag
    11461 cgggcggtct ggtcgcggcc gcggtccctc gtggacgtca agctcgtcgg tcgggcgacc
    11521 ggggccaccg tcaacgacat cctcatcggc gcggtgtccg gggccatcgc cacctacgtg
    11581 gacacgcacg gcggcgaccc cgaagacctc acgacgatgg ttccggtcaa cctgcgcgac
    11641 ctgcgcgagc ccctgccccg tgagctgggc aacaagttcg ccctcgtgat gctcccgctg
    11701 cccacgggcc ggctggcacc cctccaacgg ctggccgagg ccaagtcgcg gatggacacg
    11761 atcaagcact cgcccgaggc cgtgctcacc ttcggcctga tcaacgccat cggccgcacg
    11821 aacaccgaga tcgccaagca ggccatcgac ttcttcgccg ccaaggccat cggcgtcacg
    11881 accaacgtcg ccggcccgat gaccgggcgc tacctcgccg ggacccgcat cacgagcatc
    11941 ctcgggtggg tacccggctc gggccggcac accctgggcg tgtgcatctt cagctacgac
    12001 ggcgtcgtgc gggtcggctt caaggcggat gccggtgtcg tcaccgaacc ctccggcctg
    12061 gtcgacgcgt tcgacgagga gatggacacc ctcgtccgac tcgccgccgc cgtctgacgg
    12121 tcccgaaacc accaggaggt cgccatgccc accgacaccg ccagcgccac ggtcgtcgtc
    12181 gatgcaccgc tcgaggaggt gctcgcgacg atccgtgacg tggacggcat ccccgggtgg
    12241 gtgagcgaca tcatcgaggc ggaggtcctg acgaccaccg aggacgggat gccgctcacc
    12301 gcccggttcg cggcctccac ccccgtgggc accgaccgct acaccctcgc ctacgagcac
    12361 tcccccgacg gcctcagctg gcacctcgtg gagggtcgcc tgcagaccgc ccaggacgcg
    12421 acctacaccc tcgtcccggt cgacgacgcg cacaccgagg tcaccttcac cctgaccatc
    12481 agccacccgc tgccccttcc ggggttcatc aggagccgga ccatcaaggg cctcgtggcg
    12541 aacaacaccg gtggcctgaa gcagtacctc gcgccctgag ccggtcagtc ggcgtcaagc
    12601 acctcgcgga gcttggcggc gaaggccgcg gggtcgttgt tcggccccat cgggctcacc
    12661 gtgaacccgc cgtgcggtcg ccgaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnaacgc gcacggttcg tgtcccgatg gtgctcatgg ctgccccttc cggtggtgcg
    12841 tgaccacccg ggtcccgcac caccggaagg ggaaccgacc gacacctcaa cccttgacgc
    12901 agagcagcgt cgtgagccgg gcaaccacct cgacgaggtc ggactgtgct gcgatgacac
    12961 cctcgaggtc cttgtaggcc cctgggatct catcgagcac accggcgtcc ttgcggcact
    13021 cgacaccgag ggtctgcgcg tcgaggtcct cgagcgtgaa gtgccgcttg gccgcggtgc
    13081 gcgacatccg gcgcccggca ccgtggctgg ccgagcagaa ggatgccgtg ttgccgagtc
    13141 cgcgcacgac gtacgacccg gtgcccatcg accccgggat caggcccagc gacccggccc
    13201 cggcgaagat cgcacccttg cgggtcacga cgaggtcgac accgtcgtac gtctcctgcg
    13261 agacgtagtt gtggtggcag cgcacggcat ccgcgaaggt gacgtgcggg aacgtctcac
    13321 tgacgacgcg ccgcagggtc accatcatcg cctcgcggtt gagcatcgcg tactcctgcg
    13381 cccagtacag gtcgcgcagg taggcagcca tctcgggggt cccgtcgagg aagaccgcga
    13441 ggtcacggtc cggcaggccg aggttgtgct ccatgccctt ggcggtgttg atgtgccgct
    13501 cggcgatctc cttgccgatg ttgcgcgacc ccgagtgcag catgagccac acggcgccgt
    13561 ggtcgtcagc ggtgagctcg acgaagtggt tgcccccgcc cagggtgccg acctgagcgg
    13621 cggcacgacc ctcgcggtct gcgacccccg cgtggagacc accgaaccga gcgaacagac
    13681 cgccgaacgt ccgcgacagg gcgcggtcgg tggacagcac cggtgcgctg ccgtcgtgcg
    13741 cggtgaaccc cgtggggatc accgcctcga tggcggaccg cagcggcccg aggtcgtcgg
    13801 gcaggtcctc gacggtgagc gacgtcttca ccgcgtgcat gccgcagccg atgtcgacgc
    13861 cgaccgcggc cgggctcact gcctgacgca tggcgacgac cgagccgacg gtcgccccct
    13921 tgccgaggtg cacgtcgggc atgacgcgaa caccgtgcac ccacggcaga gccgcgatgt
    13981 tgcgcagctg gttcagcgca gccggctcca cctcgtgctc gtgggcccac atcagggtgg
    14041 gtgcggaggc gccgcgaagc tcgacgggga gcccagcggc cggggacatc tcggcgtcag
    14101 gggcgatgtt catctcgtga atcctctcgt cttggtcaat gtctgatcga cgtcctggtc
    14161 gatgtctggg tggtggtgat cgtgaaggtg gagcccccgg cgcgagtcga acgcgcggca
    14221 tcccgattcg tagtcgggcc ctcttccagc tgaggtacgg gggcgcatcg ccgtcgagac
    14281 gacgaagggc cgctccggtc acggagcggc ccttcgctgc aacagcggtg gtgactgggg
    14341 tcagtcgtca ccttggaagg gtcgcttcgg acggaagcct tgacgcgcgc cgggtcgcag
    14401 cacgtcagcg ccgagcagcg cggcgctgcc ctgccgtcgg tactggttcg ctggcatgtc
    14461 tgcggtgtcc ttcaggtggt cgcgggtgcg tcagacgagg atggcgcatg gcagcgccgg
    14521 ggcgccactg gttttcggct cagcgaggca tggcagggca agcacgaggt gcacaaggga
    14581 agaaatgcct tgcgaacctc gggcttcccc catggggagg aaaagtcagc gcggggggtg
    14641 ggtcagctgt cgaagccgat gccgacggca tccatggccc gcaaccacag gttgcgccgg
    14701 ccggtgcggt cctcggcggc caggctcgcc cgggtggccc gcaccccgac gtaccggaag
    14761 ggttcgggcg ggaagggcag cggcttcttg cgcaccatct ccagagccgt cacctcggtg
    14821 tcccggccgt cgaccaggtc gagcgccacc tgcgccccga agcgggtcga cgcgacgccg
    14881 agccccgtgt agcccacggc gtaggcaagc cgcccaccca gtgccgtgcc gaacaccggg
    14941 gtgaaccggg tcgtcgtgtc gatgaccccg gcccatcggt gcgtgaagcg cagcccctgc
    15001 aactggggga agatctcgaa gaagtgccgc gcgagcaggt cgtgcgacga cgactgctcc
    15061 aggcgcgggt cgatccgccc cgggaagtgg tagttcgcgt cgtacccacc gaagagcacc
    15121 cggtcatcca gggtgcgccg gtagtagtgg aactggttgc ccgcatcggt gagcccttcg
    15181 cgcccgtgcc agcccacggc atccagctgc gccggtgaca gcggttcggt catgagcacg
    15241 tggtcgtaga cgggaagcgt gtagtgcgcc agtcgcttca gcgggccgcg gaaggccccg
    15301 gtgccgagca cgacccggtc ggcgcgcacg cttccccact cggtgcgcac cgtgacgccg
    15361 gcgatgtcgg cgtcgaaccc ggtcaccgag gtgtcctcgt gcagccggac cccgagccgc
    15421 tcggccgcgt cggccagacc ccacaccagc cgcgccgggt cgaccaggcc caccgtcggg
    15481 tcgaggaagc ccgacaggta gcggggcgag ttgacgcggg cgcgcgcctc agcggcatca
    15541 aggagggtga ccgggatgcc gtgtgcccgg tggagctcgt agccctcacg aaccgtgccc
    15601 acctggtgct ctgtcagggc catcgtgatc tcgccgggta cgtggaagtc ggcgtcgatg
    15661 ccgtgccggg tgaggctgtc agccaggtcg gtcatgttct gcgccccgag gcgttcgagg
    15721 gtctcgatct cctcgggcca gcaggccagc ccctgcgcca ggccgtgggt cagcgagggc
    15781 gagacgaaac cgccgtttcg acccgaggcc gccacgccga accggccgcg ctcgagcagc
    15841 acgacctcgc gcgaggggtt gtcctccttg gcctggacgg cagcccacag gccggtgaag
    15901 ccgccgccga cgatgaccag gtcggcccgg tggcccgaac cggtgagcgc tgcgcgcggc
    15961 gccggccgct cgggtcggtc ggtccagaag acatcgggag tcgcatcggc gagggaggca
    16021 cgcacggccg ccgggtcggt cgtcatggcg ccatcatgcc gcgccggggc gcccgcgccg
    16081 cgacccgtca tccggatgcc gtgcgaccat ctcccgtatg ccgccgcgcc tgctcagccc
    16141 cgacgtcacc accgcctccc tcacccacga ggcgttgccg gccgacgacg tgctccacgg
    16201 gtcgccgacc gctgccgtcc tgacgctcga cgcactcccc acctgcgagg tcgggctgtg
    16261 ggagatgacg cgaggttccg ctcgtgacac cgaggtcgac gaggtgttcg tcgtggtctc
    16321 gggtcgcggc acggtgacct tcgcggacgg tgagcgactc gacctcgcgc cgggggtcgc
    16381 ggtgcggttg cgcgccggag aacgcaccga gtggacgacc accgagaccc tgcgaaaggt
    16441 ctggatcgcc tgagcgcacg gcgtcgaaat tgcttgacgc gtaaaggtct cggttcggta
    16501 gcgattgggc tgatcgtccg aatctcggct gtgaccacgc gcacacccgc gcatcagtcg
    16561 ctaggttctc tgcatgacag agccacaggt cgtccgcgac agcgccccga agcagcccct
    16621 cgtcgtcatg gagggtgtca acaaacactt cggagcgctc cacgtcctca aggacatcaa
    16681 cctcaccgtc ggcaagggcg aggtcgtcat cctcatcggc ccctccgggt cggggaagtc
    16741 gacgctgtgc cgcacgatca accggctcga gacctacgag tccggctcga tcaccatcga
    16801 cggcaagaag ctgcccgagg agggcaaggc gcttgccgac ctgcgcgccg acgtcggcat
    16861 ggtgttccag tccttcaacc tcttcgcgca caagacgatc ctcgagaacg tcacgctcgg
    16921 gccgatcaag gtccgcaaga tgaacaaggc tgacgccgac aagcgggcca tggagcttct
    16981 cgagcgggtc ggcatcgccc accaggcggg gaagtatccc gcccagctct cgggtggtca
    17041 gcagcagcgt gtcgccatcg cgcgctccct ggcgatggac cccaaggtca tgctcttcga
    17101 cgagcccacc tccgcccttg accccgagat gatcaacgag gtcctcgacg tcatggtcga
    17161 gctcgccaac agcggcatga ccatggtcgt cgtgacgcac gagatgggct tcgcgcgcaa
    17221 ggtcgccgac cgggtcgtct tcctcgcgga cggccagatc gtcgagatgg ccacgcccga
    17281 ggagttcttc accaacccca agagcgagcg cgccaaggac ttcctcggca agatcctcac
    17341 ccactgacct tcctccagca ccaccgtttc agtccccaac aagtaaggaa tcaccccatg
    17401 acgacacgac ggatcagcat ggtcgccgcg gcggccgtcc tggccctggg cctcagcgcc
    17461 tgcggctcgg acgacggcgg ctcggacggc ggggccgacg gcggttccgg tctgaagatc
    17521 ggcatcaagt tcgaccagcc cggactcggc ctgaaggtcg gcgaccagta caccggtctc
    17581 gacgtcgacg tcgccaagta cgtcgccaag gagctcggca ccgccgaggg cgacatcacc
    17641 ttcgtccagt ccccgagtgc gcagcgcgag acgctcatct ccagcggcca ggtcgacctg
    17701 atcttcgcga cctactccat caccgacgcc cgcaaggaga agatctcctt cggtggtccg
    17761 tacttcatcg ccggtcagga cctgctggtt cgcgccgatg acacctccat caccggcccg
    17821 gacaccctcg acggcaagaa gctgtgctcg gtgaccggct cgacctcggc ccagaaggtc
    17881 aaggacaagg tcgccggggt caacctggtc gagttcggca cctactccga gtgcctggcg
    17941 gcgctgctcg gcggtggcgt tgacgcgctc accaccgacg acaccatcct cgccggctac
    18001 gccgcgcagg aagccaacaa gggcaagctc aaggtcgtcg gcgccccgtt ctccgaggag
    18061 cgctacggcg tcggcctcaa gaagggcgac gtcgccctgt gcgagaaggt caacgcggcc
    18121 ctgaagaaga tggtcgagga cggctcgtgg cagaaggccg tggacgccaa cctcggcccg
    18181 gccgggtaca aggcgcctgc gggcaacccc cccacccccg acgcctgctc ctgatcgact
    18241 cgtcggacgt gtgccgcccg cttccccggg agcgggcggc acaccccgtc gcaccatgaa
    18301 aggtgagtga tgctcgagac tctcagcaac tacgacgtgg cagccgccgt ctgggtgacc
    18361 atccaactgt cagtgctgtc cgcgatcgga gcgctgatca tcggcaccat cgtggccgtg
    18421 ctgcgcgtgt cgcccgtggc cgtgctccgc gggatcggca ccagctacgt caacatcttc
    18481 cgcaacacac cactcacggt gctgctcacc ctgtgtgtgc tcgggctctc actcctcgtg
    18541 gggctgcaac tctcccagga cctctccaag gacgccttcc gctgggctgt cgtcggcctc
    18601 tccgcgtacc acgccgcatt cgtctgtgag gcgatccgca gcggcgtcaa cacggtgccg
    18661 gccggtcagg ccgaggcggc ccgctccatc gggttgacct tcacccagtc gctgcgagag
    18721 gtcctcctgc cgcaggcctt ccgggggtcc atcgccccgc tgggttcggt gctcatcgcc
    18781 ctcatcaaga acaccacggt cgcggccgtc gtcggtgtcg ctgacacggc caagctgctc
    18841 gcgaccatca ccgagaacga gggtgcccag ctgggagtct tcttcgtcat cgccatgatc
    18901 ttcgtcgtcc tgaccctgcc catcggggtg ctggcgacca gcctcagcca gcggatggcg
    18961 gtgaagcgat gagcacgcag aacgtcctgt tcgacgcccc gggcccgaag gcccgccaac
    19021 ggcacgccat cctcaccggc gtgggtgtgc tgctcgctgg cgccgtcctc tacgtgatcc
    19081 tgggacgcct cgccgacgcg ggtcagctcg acgctgccaa gtggaagccg ttcgtcaccg
    19141 attcgtcgtt gtgggtcgac tacctgatcc ccgggctcat cggcaccctg aaggcagcca
    19201 tcctgtcggt catcttcgcc ggcatcttcg gcctgatctt cggcatgggc cgcctctcgc
    19261 acatcaaggc catccgctac gtcagtggtg cggtcgtcga gttcttccgc tcggtgcccg
    19321 tgctgatgat gatgatcttc ctgtacttcg gctacttcgc gacgagcacg atcgtgccga
    19381 gcagccaggg cccactggcc gcggtggtcc tcgccctgac gctgtacaac ggctcggtca
    19441 tcgccgagct cgtgcgctcc ggcgtgcact cactgcccaa ggggcagtcg gaggcgggcc
    19501 tgtcgatcgg gctcaccccg tcgcagaccc tgcgcacgat ccagctgccg caggcgctga
    19561 cggcgatgct gccggccctg atcagccagt tcgtcgtcgt gctgaaggac tccgccctcg
    19621 gcaccgccat cacgtaccag gagctgctga cgtggtcgaa gaccgctggc tccgcgtacg
    19681 ccaacacggt gccggccctg atcgtgtgtg ctgccctgtt catcgcgatc aactacggcc
    19741 tgacccgggt ggccacctgg gtggaacgcc ggctcaaccg ccgcggcacc accgcggggc
    19801 ccgtcaccac agtggcgccc acgatgatcc aggggcaggg tgagcccggc gggccggccg
    19861 cccacggcac ccgcatcatc ggcgccggga tcgagtcggc ggaggaagcg gcatccgact
    19921 tcgtccagtc cgcacgcgag cgtcgcaagg gccacggcga cgacacctga gcccgaccat
    19981 gcagaacggc cccactccgc caaggagtgg ggccgttcgc gtgctccccc ggttggactc
    20041 gaaccaacaa cctgccgatt aacagtcggc tgctctgcca attgagctac aggggaaagg
    20101 ccaccacggg atcgccgcgg tagcgctcgc acaccctagc aaaggtcatg agcagcgacg
    20161 aaaacggccc gaaatcagtc gagtccgacc tgctcgcgca ctcttcgcag cccgtcggag
    20221 acctgctcgg ccacaccggg atccgcgctg cgcacacccg ggccgaggac cgcctgactg
    20281 agcagcgcac cggtggcgag gtccttgcgg accacgacct tggcggtccg ccggcccccg
    20341 agatcgaggg tctcggcgag cacgaccgag gcctggacgc gctctcgcag ggtctcgggg
    20401 aacatcccga gctcggtgag ctggaaacgc gtcggtcgtt gcccgtcgac ccaggtgatc
    20461 ttgaggatgc cgtcctcacc accccacgac ccggcgtcga ccaggtgcca cggacgcgac
    20521 agcgtcaccc gcgcgccttc ggggtccggc gccacggcat acagccggtg gcgcccggct
    20581 accacggtgg ctccggtgcc cacgtcatgg gcccaggcca gcagcttctc gccacctgcg
    20641 gggtcgatgg cctcggcgac ctcggcgggc accgcgggac ggcgcaggcc gctccactcg
    20701 agcctcatga cgtgttcgcc tctcgcaggg cgacgcggtc gagttcgaga cggtgcaagg
    20761 ccatggagcg ctcctggaga tcagcgggat cagcatctgg gtcgttctgc agtcggcgca
    20821 ccgacgccat ggcgtcggcg atgcgacggc cgaggtgggc gtctctgaca ccggcgacga
    20881 gcgagttgag gtagcgctgc ggcggcagcc ccgtcgacgg gtcgaacttc gtgggcagcg
    20941 gagccaccga caactgcgcg acgaagccac gcacccccag ggcgcaggca tcggtcacgg
    21001 cggtcaccca cgccatcgtc gacgctcggc gggcagcgtg gccggcgatg cggatgccgt
    21061 cgaacaccgc ccggtggacc ggggcggtga acgcgtcggg gacgagcaca tcgatgtcgg
    21121 cgtcggtgaa ctgctggggg tactggatga gggcctggag cagctgccgt tcggtgagga
    21181 ccaccggatc gcgcaggtcg ggggccggga gctgcgcgag ctcgaacgga ccgggctcga
    21241 ggacctcgtc ggtactggcc ggtcggggct gccggtcggc gctgtcgtcg tgcttgagcc
    21301 ggcccgcctt cttgacctcg gccgccagct gttcgacctc gatgccgagc cagccggaca
    21361 ccgtgcgggt gtactcgggc cgcaggctgg tgtcgcggat ggacccgacg atcggggcca
    21421 ctgccttcat cgcctgaaca cgaccctcgg cggtctcgag gtcgaagcgg cggatcgtcg
    21481 tgcggacggc gaactcgaac atcggaacgg cgtcatcgac gagagcgcgc accgacgcgt
    21541 cacccttggc cagtcgaagc tcacacgggt ccatgccgtc cggtgccacg gcgacgaacg
    21601 actgcgcggc ccaccgctgg tcctccgcga aggcccgcat ggccgccttc tgaccagcgg
    21661 catccccgtc gaaggtgaac accacgcgag cctgtgggcc gtcggtctcg tcgcgcatga
    21721 tgcggcgaag gatcttgatg tggtcgatgc cgaacgacgt cccgcacgtg gcgaccgcgc
    21781 cctcgacgcc ggacaggtgc gccgccatga cgtcggtgta gccctcgacc accacggcga
    21841 ggcgctgagt ggagatcgcc ttcttggcgg catccaggcc gtagagcacc tgggacttct
    21901 tgtagatcgg cgtctcggac gtgttcaggt acttggccgc gatgcggtcg tcgtcgagga
    21961 cgcgccgggc gccgaagccc acggtgtcgc cggtggtgtc cctgatcggc cagaccagcc
    22021 gcccccgaaa gcggtcgtag agcccgcgac tgccgcggcc ggtcaggcca ccggtgacga
    22081 tctcgtcctc ggtgaagccg cgcccgcgaa ggtgacgcgt gagcgcctca ccgctgcgcg
    22141 gggcgaaccc gatgccgaag cgctccgcgg acgcgccatc gaagccgcgc tcgcgcagga
    22201 agtcccgacc gggcctggcc tccgcagtgg tgcggtcctg caggacctcg gcgtagaact
    22261 cctgggcgac ccggtgcgcc tcgatcaacc gcgagcgctt gccgagcgag accccaccgt
    22321 cgcggggacc accgccctcc tcgtagcgca gctcgaagcc gatcttgctg gccagccgtt
    22381 cgatggactc ggtgaaggtg aggtgctcga ccttctggac gaaggagatg acgtcgccgc
    22441 cctcgccgca cccgaagcag tggtaactac cgaccgcggg tcgaaccgtg aaactcggtg
    22501 acttctcgtc gtggaagggg cacaacccct tcagcgagcc gaccccggca ggacgcagcg
    22561 tcacgtggtc ccggatgacg tcctcgatgg acgtgcgctc cttgaccgcc gcgatgtctt
    22621 ctgccctgat ccgacccgcc acgaaccatc ctttccggtg tgccgtgagt ctaggtcgtc
    22681 acaccgac
//
LOCUS       DAOSOC010000024        22489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_2310_length_22489_cov_9.205804, whole genome shotgun sequence.
ACCESSION   DAOSOC010000024 DAOSOC010000000
VERSION     DAOSOC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 22489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22489
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_22489_cov_9.205804"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PL014_03525"
     CDS             complement(<1..533)
                     /locus_tag="PL014_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT56190.1"
                     /translation="MTSNHSISGRADALADDTTFDESGFVLDDDASFDGDQLDREERA
                     ALRRVGGLSTELEDVTEVEYRQLRLERVVLASVWCEGTAEDADNSMRELAALAETAGS
                     EVLAGLVQRRANPDPATYLGKGKAAELRDIVIAEGADTVVCDTELAPSQRRALEDVVK
                     VKVIDRTALILDIFAQHA"
     gene            610..1215
                     /locus_tag="PL014_03530"
     CDS             610..1215
                     /locus_tag="PL014_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT56191.1"
                     /translation="MTENDHYFTADPASPNERRRVRVRLAGRPVEVEVAPGIFSPGGL
                     DKGTAVLLDEAPPPPATGTFLDLGCGWGPIALTLALSSRNATVHALDVNERALDLTRR
                     NAAALGLERVTATTADGIPDDVRFDLIWSNPPIRVGKAVLHDLLRTWLTRLAPGGVAY
                     LVVQKNLGSDSLQRWIESELGMPCGRFASSKGFRVLEVRTP"
     gene            1212..2096
                     /locus_tag="PL014_03535"
     CDS             1212..2096
                     /locus_tag="PL014_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT56192.1"
                     /translation="MTPWLGIVLVPTLPPEALRPLAAAADRHLDELWVWEDCFKESGV
                     AAAAAALAWTDRVQVGIGLAPVPLRNVALLAMEVATVHRLFPGRFLPGIGHGVQDWMG
                     QVGARERSPLTLLREHAQAMRLLLDGHEVSTSGRYVHLDRVKLDWPPEPGSPLLIGGG
                     GPKTLELAGRYGDGILIGSAVSESELEASVAAGRLGWAQGRGADGTPMPVLAHLIAAT
                     GPRAHQRVEEELARWGQTDGAPGRGVAGDAATIADAVRRLAAIGATTVVFQPTRDEPD
                     LDGFVEFLGTDVRAALDG"
     gene            complement(2074..2964)
                     /gene="dapF"
                     /locus_tag="PL014_03540"
     CDS             complement(2074..2964)
                     /gene="dapF"
                     /locus_tag="PL014_03540"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOT56193.1"
                     /translation="MAERLTFTKGHGTENDFVLVPDLEGVLDLTAPLAAAIADRRAGV
                     GGDGVIRVVPTEAASDQAVRDQAPHARWFMDYRNADGSLSEMCGNGTRVFAAFLRREG
                     LESADEFAIATRAGVKHVRVDGDGFAVGLGPWRLGDPATAQRDGFDALVHLDDLEPFS
                     ALTVDVGNPHTVVALPEAVDLDAVDLATAPAVRPEPAHGTNVEVVRPLGPGHVAMRVF
                     ERGVGETRSCGTGACAAAIATSFWAGSLDTGSTWVVDVPGGRLTVRLLPDQGVELAGP
                     AALVADGVIDLAQLSRRAQP"
     gene            complement(2957..3919)
                     /gene="miaA"
                     /locus_tag="PL014_03545"
     CDS             complement(2957..3919)
                     /gene="miaA"
                     /locus_tag="PL014_03545"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT56194.1"
                     /translation="MSTDGEPFVVAVVGATATGKSDLGIALAQLLDGEVVSADASQLY
                     RGMDIGTAKVPLAQRHGIAHHQIDVLDVTDEATIAGYQRHARADVDAVLARGRVPVVV
                     GGSGLYVRALLDHLDLPPTDPDVRQRLEERADAEGTTALLAELRELDPVAAAAIEPNN
                     TRRIVRALEVIELTGRPFSATMPTRQTLRPTVFVGLRVDREALDRRIERRTRQMFASG
                     LVQEARGLVGAGLREGRTASRAVGYAQALAVIDGSMSVEEAEADTALRTRRLVRRQES
                     WFGADPRIEWFDPSEPDLVDRVARTIEDAASTARRARCDDEPHG"
     gene            complement(3909..4514)
                     /gene="def"
                     /locus_tag="PL014_03550"
     CDS             complement(3909..4514)
                     /gene="def"
                     /locus_tag="PL014_03550"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT56195.1"
                     /translation="MPIRPVVVTGEPVLHQRAQPVEVIDDEVRALVADMFETMDAARG
                     VGLAAPQIGVGLRIFTWQLANDDGIPERGVVINPYLTSAKPPVGDPDPHDEVEGCLSV
                     PGESFPLRRGESASLVGQDLDGNEIRYEATGWFARMLQHEYDHLNGFLYVDRLTGKWA
                     RRARKAVKANGWGEPGISWTPGVDRDPFGHDDDPPEEERVD"
     assembly_gap    4619..4718
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4719..5065
                     /locus_tag="PL014_03555"
     CDS             <4719..5065
                     /locus_tag="PL014_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT56196.1"
                     /translation="DGRLERITVRDTRSGEQEVLGCRGLVLLLGAEPHCEWLPAEVAT
                     DGRGFVLTGRDVPTDRWTDGIPPASLATTVPGVIAVGDIRATSMKRVAAASGEGASVV
                     PLVHAWLDPDQG"
     gene            5192..5380
                     /locus_tag="PL014_03560"
     CDS             5192..5380
                     /locus_tag="PL014_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HOT56197.1"
                     /translation="MNIFDSAKEKLAELTETHGDQVESISDQAIEKAGDAADQATGGQ
                     FAEHVDTAQSAADGAIGT"
     gene            5538..5732
                     /locus_tag="PL014_03565"
     CDS             5538..5732
                     /locus_tag="PL014_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56198.1"
                     /translation="MAHNTSGLPPLDIEPFDPAPLYAAANSPERRVDPARERARSVRA
                     LGLVLALAAVGVVAAVLALG"
     gene            complement(5738..7252)
                     /gene="miaB"
                     /locus_tag="PL014_03570"
     CDS             complement(5738..7252)
                     /gene="miaB"
                     /locus_tag="PL014_03570"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOT56199.1"
                     /translation="MTPKTYDVRTHGCQMNVHDSERLAGLLETAGYVDLASVPAAERP
                     ETADVVVFNTCAVRENADNKLYGNLGQLRPAKTANPDLQIAVGGCMAQKDRESIVKRA
                     PWVDVVFGTHNIGSLPALLDRARHNKRAEVEILESLETFPSTLPTRRDSAFAGWVSIS
                     VGCNNTCTFCIVPSLRGKEADRRPGEILAEIEALVAQGVIEITLLGQNVNTYGVEFGD
                     RFAFGKLLRACGEIEGLERVRFTSPHPAAFTDDVIEAMAATPNVMPSLHMPLQSGSDT
                     VLKAMRRSYRSEKFLGIIDKVRTAIPDAAITTDIIVGFPGETEADFAETLRVVEASRF
                     SSAFTFQYSIRPGTPAATMPNQVPKAVVQERFERLIELQERVSWAENRTLEGREVEVL
                     VAPGEGRKDGETARLSGRARDNRLVHFAVPDGAERPRPGDVVTVGVTYGAPHHLVADS
                     GVQGGAYSVRRTRGGDAWASLQDAPVPGKPAVSLGMPSLGKPATSPTATAPCAT"
     gene            7542..7877
                     /locus_tag="PL014_03575"
     CDS             7542..7877
                     /locus_tag="PL014_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT56200.1"
                     /translation="MPVPLYQAKAEFFRTLGHPARIRVLELLAERDRAVHELLAAINV
                     EAPSLSHQLAVLRLGGLVTQRREGGQVIYSLALPAVRDLLASAREILQTKTADQGGLV
                     DDITPEPRA"
     gene            7874..>8779
                     /locus_tag="PL014_03580"
     CDS             7874..>8779
                     /locus_tag="PL014_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOT56201.1"
                     /translation="MSTDTARRAVMERLDAVRPRSRDLRAMVATPRQDVLAGVTVGVV
                     ALPLALAFGIASGLGATAGLVTAVVAGVVAALFGGSHVQVSGPTGAMTVVLVPIVATH
                     GAEGVLVVGLMAGVILVVMGLAGAGRYVRFIPLPVIEGFTLGIAALIALQQVPSALGL
                     SARPEHVVVSAVAAVLEWFGSPDLAPVAISVGVAAAILVLSRLRPAVPVALLGVVVAT
                     VAVRLADVPVETIGALPDRLFTLTVPSVSWSDLRVLAVPAVAVAALAALESLLSATVA
                     DAMSVSSRHDPDRELFGQGLANLASP"
     assembly_gap    8780..8879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8880..9564
                     /locus_tag="PL014_03585"
     CDS             <8880..9564
                     /locus_tag="PL014_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT56202.1"
                     /translation="LTDVVAWIPLAALAGVLIATAVRMIEVSSLRALARASRGDAAVL
                     GLTFLATVVLDLATAVVLGIVTAGGLALRQLARTTALHEESLPQKYLDEEEHGAQETA
                     LLRERVVAYRLEGPLFFGGAHTALLELTEVSDVKVVILRMSHVSTLDATGAAVLADTI
                     RSLEARGVTVLLSGMPQRFVPRLAATGIHQHLVTLNHVFDSTPEAIAHARRHVARDHH
                     HDEGVRPGG"
     gene            complement(9548..10387)
                     /locus_tag="PL014_03590"
     CDS             complement(9548..10387)
                     /locus_tag="PL014_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOT56203.1"
                     /translation="MRRWRIVHTTSLTYDGQVRAAHNELRMTPVNEAGQTTLENRIRI
                     RPMTWSHVYRDHWGTHVMAMESLASHGQLEIEAISTVERTDVARHTPSLDWAAVRDPA
                     VQDRSYEWLMLSARTRPGDGIAELVDAVRGRETPRVAAEELASLVRAEMSYVPGSTSV
                     HSRGEDAWAERSGVCQDFAHITVGALRSLGLPARYVSGYLVPVRDTAVGETSKGESHA
                     WVEYWDGGWTPVDPTNGTEVGVDHVVVARGRDYEDVPPFKGIYSGRATASLGVVVAIT
                     RLA"
     gene            complement(10389..11315)
                     /locus_tag="PL014_03595"
     CDS             complement(10389..11315)
                     /locus_tag="PL014_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOT56204.1"
                     /translation="MLSRIAESLFWIGRYTERADGTARIVDVLRLQLLEDPGADEVTA
                     SRTVLSVIMGMPFEGDPGFADVGAALVFDRSNASSITGALFAARENARRARETLSTEL
                     WEGINTTWHRWNGFGRTVTTERHLSWVRERAALVSGIADSTMSHDEAWDFLVLGRSLE
                     RADMTARLVATGGLPRGGAPWSVVLSSCGAQQAFMRSQRGLLSDDRAAAFLVLDREFP
                     RSVFFALSEAERRLDALSPQEDRIGISDEARRQLGRIRTSLEYRSTSDVIADLPAQML
                     RVQGSVMAASQAVASRYFQSGQVTTWTRDGVV"
     gene            complement(11309..12940)
                     /locus_tag="PL014_03600"
     CDS             complement(11309..12940)
                     /locus_tag="PL014_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOT56205.1"
                     /translation="MPAEAPGGLFDDYALGQAWDEMLSEPGAPRTPYKPVFHTLRGMN
                     GQALKERSDSLARSYLDQGVTFDHAGEERPFPLDAVPRVISAHEWQIIESGVVQRVTA
                     LESFLDDIYSREGEIPRAVHEGIIPWRLIASSKHYHRAVMGIRPSNGVRVHVAGVDLI
                     RDEVGTFRVLEDNVRVPSGVSYVIANRRAMANVFPEAFATMRIRPVHDYPRMLLAALR
                     AAAPDGASDPTVVVLTPGVFNSAYFEHALLARMMGVELVEGRDLVCVGGQVRMRTTTG
                     DRPVDVIYRRIDDEFLDPVHFRGDSILGVAGLVSSMRAGRVTVVNAIGNGVADDKLIY
                     SYLPELIRFYLDEDPILGNVDTFRCDEPKALQHVLENLHDMVVKPVDGSGGKGLVVGP
                     KADAETLERLRAQLTADPRGWIAQPVVQLSTVPTFIDGRLAPRHVDLRPFAVNDGERV
                     RVLPGGLTRVALPEGELVVNSSQGGGSKDTWVLSDRRAATSTSSRPPDNREVVVMSAP
                     TASHEDSMKAQQQQQQQQGHRTVAHADTRHEESSC"
     gene            13057..13515
                     /locus_tag="PL014_03605"
     CDS             13057..13515
                     /locus_tag="PL014_03605"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT56206.1"
                     /translation="MTQPGPTVSLRPVDAGSWRAVAGLTIAPGQDAFVATPTHYLALC
                     AYEDVWRPLAVHDEEGAVIGFLMWGIDDEDGSCWLGGVIVDAAHQGRGVGRAAMAQAL
                     ATLRPRDDDAGFALAYHPQNTVAKGLYASLGFVETGETDDDEVVARRRPA"
     gene            13626..14036
                     /locus_tag="PL014_03610"
     CDS             13626..14036
                     /locus_tag="PL014_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOT56207.1"
                     /translation="MRTLLNIIWFVFGGFWLAVGYAFAGLVLCLLIVTIPLGVAAFRM
                     ASYAIWPFGRAVVQQPDAGAGSVVLNILWIVLAGWWLALEHVITAVAQAITIIGIPLA
                     IANLKMIPISLFPYGKQIVPTDSLSAAQRPLHSL"
     gene            complement(14146..14469)
                     /locus_tag="PL014_03615"
     CDS             complement(14146..14469)
                     /locus_tag="PL014_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HOT56208.1"
                     /translation="MSEEPLPPHETLRKARELIDAGRPFAAHEVLEARWKAGPAEEAD
                     LWQGLAQVCVALTHAARGNQVGAQRLFERAGDRLSAFASTGRDAYGVDVTKSAAQARR
                     HRGTF"
     gene            complement(14624..>14917)
                     /locus_tag="PL014_03620"
     CDS             complement(14624..>14917)
                     /locus_tag="PL014_03620"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT56209.1"
                     /translation="ARYAIGLYDPSFFGRGLGRQATRLVLQHAFETLMLHRVDLRVLD
                     FNHRAIATYRACGFVEEGRERETCFLDGRWYDDVVMAVLEHEFAVARGGRRET"
     assembly_gap    14918..15017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15123..15680
                     /locus_tag="PL014_03625"
     CDS             15123..15680
                     /locus_tag="PL014_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOT56210.1"
                     /translation="MRPLRYSINVTLDGCCHHEAGLPPDEESMRYWTAEMQRADALVF
                     GRVTYEMMESAWRKPATGTWPDWMGEREIPFAETIDRAKKYVVSSTLSEVDWNAELVR
                     GDLGHAVQQLKHEPGEGLFVGGVTLPLALADLGLIDEYAFLVQPVLAGHGPTLLAGLR
                     ERIQLELVDRQEFRSGAVALRYRPT"
     gene            complement(15701..16300)
                     /locus_tag="PL014_03630"
     CDS             complement(15701..16300)
                     /locus_tag="PL014_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOT56211.1"
                     /translation="MPFADSTTPPPSWAVGSPTAGEPVVGSPPPPDPRTRGSEPNPYD
                     WARQIVLRQLTAAPRSRAQLEQALRKKDCPDEIAAAVLDRMEEVGLVDDTAYAGMLVR
                     SQQAGRGLARRALAQELRRKGVDDETAQAALDEVDPHDEEDRARALVAKKMRSISGLT
                     PLVQTRRLAGMLARKGYPSDVSMRIVREAVREAPENQRD"
     gene            complement(16318..17382)
                     /gene="recA"
                     /locus_tag="PL014_03635"
     CDS             complement(16318..17382)
                     /gene="recA"
                     /locus_tag="PL014_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT56212.1"
                     /translation="MPNPTAAANGGEKDKAIASVMAQIEKQHGKGAVMRLGDDVRPPI
                     EVIPTGSIALDVALGIGGLPRGRVVEIYGPESSGKTTVALHAVANAQKAGGIAAFIDA
                     EHALDPEYAKALGVDTDALLVSQPDTGEQALEIADMLIRSGALDIIVIDSVAALVPRA
                     EIEGEMGDSHVGLQARLMSQALRKITGALSTTGTTAIFINQLREKIGVMFGSPETTTG
                     GKALKFYASVRLDVRRIETLKDGTDAVGNRTRCKVVKNKVSPPFKQAEFDIIYGQGIS
                     REGGLIDMGVEQGFVRKAGAWYTMDGDQLGQGKENARTFLKDNPDLADELEKNIKDKL
                     GIGVPRVDALAAVPDIPVDF"
     gene            17697..19412
                     /gene="sulP"
                     /locus_tag="PL014_03640"
     CDS             17697..19412
                     /gene="sulP"
                     /locus_tag="PL014_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HOT56213.1"
                     /translation="MTVATTFPGITVWRTYQRGWLRGDLLAGVTVAAYLIPQVMAYAQ
                     IAGLPPAAGLLAIIAPTLVYAVLGSSRQLSVGPESTTALMTATAVAAVGVSGPDAYAA
                     LAAALAVVTGALCLLGWAFGLGFLADLFSKPVLVGYLAGVAVLMVVSQIDSLTGIEVS
                     GDSMPAEVWSALTHLSDVHAPTLVLSSTLLVLLLLSAHFFPRLPNPLLAALLGAAAVM
                     VLGLDELGVAVVGPLPSALPQLSLPDLSPDAVATMLAPALGIAVVAYSDNVLTARAFA
                     ERSGQQIDANQEFLALGAANIAGGLLSGFPVSSSGSRTAIGASMGSRTQLYSLATLAT
                     VLVAVLALGPVIAAFPKAALGALVVYAAIRLVDVAEIRRVARFRRSELVLLVATTLGV
                     FVFGALYGILLAVALSLGDLLRRVARPHDGILGYVRGMAGMHDVDDFPDAVQVPGLVV
                     YRYDSPLFFANAEDFHTRALAAVDDADDPVEWLIINAEANVEVDLTSLDALARLHDDL
                     VARGVVLAMARVKQDLLDDLEAAGLDHAIGTEHFYPTLPTAVTAYLAWHAETHGTPHP
                     FGQVP"
     gene            complement(19425..22220)
                     /locus_tag="PL014_03645"
     CDS             complement(19425..22220)
                     /locus_tag="PL014_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOT56214.1"
                     /translation="MSDRLKMMHDASHYALLPQAVAVPVDGSEVGALFAVSAANGIPM
                     TFRSGGTSLSGQAGTDGILVDTRRHFRSIDVLDDGLRVRAGPGATVRAVNARLARHGR
                     KLGPDPASEIACTLGGVVNNNSSGMACGTVANTYNTLDSLVLVLPSGTVLDTGAPDAD
                     ERLRQLEPELYAGLDRLRDRVRGNPASVRLLEQQFSMKNTMGYGLNSFLDHTRPVDVL
                     AHLVVGSEGTLAFVASVVMRTVPLLPHAMTGLLVFDDLAGATGSLSAILETAPATIEL
                     MDAVSLRVGQADPRADEALKRIAVDRHAALLVEYQASTADEVTAMGREALPALARLRL
                     SSPAALASDPASRARLWTTRKGLYAAVAGARPSGTTALLEDIVVPVPALLPTCERLIE
                     LFAKHGYEDNVIFGHAKDGNIHFMLNERLEGGRMDRLMAFTDEMVDLVLGFDGSLKAE
                     HGTGRMMAPFVRRQYGEELHDVMREVKRLLDPRGVLNPGVIISDDRESHTRDIKVTPT
                     VEAEVDRCVECGYCEPVCPSRDLTTTPRQRIVLRREMQAAAAAGDLALLHELEDDYEY
                     DAVETCAVDGMCQTACPVDINTGDLTKRLRAEAVGPMASRGWRLAAGHWGATTRVASA
                     ALTMARVLPDPVVLVPNALVRRAGDDDVVPLWSPELPGGGRQRPEGRAPWAPAVSERG
                     EGPREAVYFSACVGTLFGPSDAGPGVRGAFLSLCRRAGVVLVAPGNLPGLCCGTPWRS
                     KGMLEGAQAMEQRVAASLWEATRGGELDVVSDAASCTEGLVTTLSHAAETDARYAGIR
                     VRDAVAYVAGELLPDLLTELPAPRRLQRLALHPTCSSTQLGLDPALMAVARAVADEVY
                     VPDSWGCCGFAGDRGLLHPELTASATQVQAAQIAEAGPFDAYASCNRTCELGMTRATG
                     EQYQHVLELLEVATRAT"
     gene            complement(22268..>22489)
                     /locus_tag="PL014_03650"
     CDS             complement(22268..>22489)
                     /locus_tag="PL014_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOT56215.1"
                     /translation="TLVPDTHVCVIRADQVVHDVPESVARLRPGPDGARPLTWISGPS
                     ATSDIELERVEGVHGPRTLVVVLVAGADS"
BASE COUNT         3006 a         8024 c         7829 g         3330 t
ORIGIN      
        1 gcgtgctggg cgaagatgtc gaggatcagg gccgtgcggt cgatgacctt gaccttgacg
       61 acgtcctcga gggctcgccg ctggctcggt gcgagctcgg tgtcgcagac cacggtgtcg
      121 gcgccctcgg cgatgacgat gtcgcgcagc tcggcggcct tgcccttgcc gaggtaggtc
      181 gcggggtcgg ggttggcgcg ccgctggacg aggcctgcga gcacctcgga cccggccgtc
      241 tcggcgaggg ccgccagctc gcgcatcgag ttgtcggcgt cctcggcggt gccctcgcac
      301 cagaccgagg ccagcacgac gcgttcgagg cgcagctggc ggtactcgac ctcggtgacg
      361 tcctcgagct cggtcgagag cccgccgacg cggcgcaggg cggcgcgctc ctcgcggtcg
      421 agctggtcac cgtcgaagga ggcgtcgtcg tcaaggacga agccggactc gtcgaaggtc
      481 gtgtcgtcgg cgagggcgtc ggctcgcccg gagatgctgt ggttcgaagt catgttctcc
      541 ctagggtctc accgggcaac gccccacggc gaccgctttc ttccgcgcgg cgctagcgtg
      601 tgccctgcga tgactgagaa cgaccactac ttcacggccg acccggcgag cccgaacgag
      661 cggcgccggg tgcgggtgcg ccttgccggg cgccctgtcg aggtcgaggt cgcgcccggc
      721 atcttctccc ccggcggcct ggacaagggc accgcggtgc tgctcgacga ggcgccccct
      781 cccccggcca ccggcacctt tctcgacctc ggctgcggct gggggccgat cgccctgacc
      841 ctcgccctgt cctcccggaa cgccaccgtg cacgccctcg acgtcaacga gcgcgccctc
      901 gacctgaccc gccgcaacgc cgccgcgctg ggactggagc gggtcaccgc gacgacggcc
      961 gacggcatcc ccgatgacgt gcgcttcgac ctcatctggt ccaacccgcc gatccgggtc
     1021 gggaaggccg tgctgcacga cctgctgcgc acgtggctga cccgtcttgc gcccgggggc
     1081 gtcgcctacc tggtggtgca gaagaacctg ggctccgaca gcctccagcg ctggatcgag
     1141 agtgagttgg ggatgccgtg tggccggttc gcgtcgagca agggtttccg ggtcctcgag
     1201 gtgaggacgc catgaccccc tggctcggga tcgtgctcgt cccgacgctg cctcccgagg
     1261 cgctgcgccc gctggctgct gccgccgacc gtcacctcga cgagctctgg gtgtgggagg
     1321 actgcttcaa ggagtcgggg gtcgcggcgg ctgcagcggc gctggcctgg acggaccggg
     1381 tgcaggttgg catcgggttg gccccggtgc cgctgcgcaa cgtggccctg ctcgcgatgg
     1441 aggtggcgac ggtgcaccgg ctcttcccgg gccgcttcct gcccggcatc ggccacgggg
     1501 tgcaggactg gatggggcag gtcggcgccc gggaacgctc tccgctcacg ctgttgcgcg
     1561 aacacgcgca ggccatgcgg ctgctgctcg acggccacga ggtgagcacc tcgggtcgct
     1621 acgtgcacct cgaccgcgtg aagctcgact ggccgccgga gccgggcagc ccgctgctca
     1681 tcggcggcgg tggcccgaag accctcgagc tcgcgggccg gtacggcgac ggcatcctca
     1741 tcgggtcggc cgtctccgag agtgagctcg aggcctccgt ggccgcaggt cgactcggct
     1801 gggcgcaggg ccgcggcgcc gacggcacgc cgatgccggt gctcgcccac ctcatcgccg
     1861 ccaccgggcc ccgggcccac cagcgcgtgg aggaggagct ggcgcggtgg ggtcagaccg
     1921 acggcgcccc cggccgcggg gtcgccggcg acgccgcgac gatcgcggat gccgtgcgcc
     1981 ggctcgccgc gatcggcgcc acgaccgtgg tgttccagcc gacccgggac gagccggacc
     2041 tcgacgggtt cgtcgagttc ctcggcaccg acgtcagggc tgcgctcgac ggctgagctg
     2101 cgcgaggtcg atgaccccgt cggccacgag cgccgccgga ccggcgagct cgacgccctg
     2161 atccgggagc agccgcacgg tgagccggcc acccggcacg tcgaccaccc aggtggagcc
     2221 ggtgtcgagg gacccggccc agaagctcgt cgcgatcgcc gccgcgcagg caccggtgcc
     2281 gcaggagcgg gtctcaccga ccccgcgctc gaagacccgc atcgcgacgt ggccggggcc
     2341 gagggggcgc acgacctcga cgttggtgcc gtgagccggc tcggggcgca cggccggggc
     2401 ggtcgcgagg tcgaccgcgt cgaggtcgac ggcttccggc agggcgacga cggtgtgggg
     2461 gttgccgacg tcgacggtca gggccgagaa cggctcgagg tcgtcgaggt gcaccagcgc
     2521 gtcgaacccg tcgcgctggg cagtcgccgg gtcgccgagg cgccacgggc cgagcccgac
     2581 ggcgaacccg tcgccatcga cccgcacgtg cttgacgccg gcgcgggtgg cgatggcgaa
     2641 ctcgtcagcg ctctccagcc cctcgcggcg caggaacgcc gcgaagacgc gggtgccgtt
     2701 gccgcacatc tccgacaggc tgccgtcggc gttgcggtag tccatgaacc agcgcgcgtg
     2761 cggtgcctgg tcgcgcaccg cctggtcgct ggccgcctcg gtcggcacga cacggatcac
     2821 cccgtcgccg cccacccccg cacgacggtc ggcgatggcc gccgccagcg gtgcggtgag
     2881 gtcgaggacg ccctcgagat caggcacgag cacgaagtcg ttctccgtgc cgtgcccctt
     2941 ggtgaaggtc agccgctcag ccatggggct catcgtcgca cctggcccgc cgtgcggtgg
     3001 acgccgcgtc ctcgatcgtt cgcgccaccc ggtccacgag gtcgggttcg gaggggtcga
     3061 accactcgat gcgcgggtcg gcgccgaacc acgactcctg ccgccgcacc aaccggcgag
     3121 tccgcagcgc ggtgtcggct tcggcctcct cgaccgacat ggatccgtcg atgaccgcga
     3181 gcgcctgcgc gtagccgacc gcgcggctcg ccgtgcgccc ctcgcggagt ccggcaccca
     3241 cgaggcctcg ggcctcctgg accagacccg aggcgaacat ctggcgggtg cggcgctcga
     3301 tcctgcggtc gagtgcctcc cggtcgacgc gcaggcccac gaagaccgtc gggcgcaggg
     3361 tctgcctggt cggcatcgtc gcgctgaacg gccgcccggt gagctcgatg acctccagcg
     3421 cgcgcacgat ccgccgggtg ttgttcggtt cgatcgccgc cgccgcgacg gggtccagct
     3481 ccctcagctc ggcgaggagg gccgtcgtgc cctcggcgtc tgcgcgctcc tcgagccgtt
     3541 gccggacgtc gggatcggtc ggagggaggt cgaggtggtc gagcagtgcc cggacgtaca
     3601 gacccgagcc gccgacgacg acagggaccc gccccctcgc gaggaccgcg tcgacgtcgg
     3661 ctcgtgcatg gcgctggtag cccgcgatcg tcgcctcgtc ggtgacgtcg aggacgtcga
     3721 tctggtggtg cgcgatgccg tgccgctgcg cgaggggcac cttggccgtg ccgatgtcca
     3781 tgccccggta gagctggctg gcatcggccg agacgacctc gccgtcgagc agctgcgcca
     3841 gcgcgatgcc gaggtcggac ttgccggtgg cggtcgcccc gacgacggcg acgacgaagg
     3901 gctcgccgtc agtcgacacg ctcctcctcg ggcgggtcgt cgtcgtggcc gaaggggtcg
     3961 cgatccacgc ccggcgtcca cgagatcccc ggctcacccc acccgttggc cttgaccgcc
     4021 ttgcgcgccc gccgggccca cttgccggtg agccggtcga cgtagaggaa accgttgagg
     4081 tggtcgtact cgtgctggag catgcgggcg aaccagccgg tcgcctcgta gcggatctcg
     4141 ttgccgtcga ggtcctggcc gacgagggag gcgctctccc cccggcgcag cgggaacgac
     4201 tccccgggga ccgacaggca gccctcgacc tcgtcgtggg ggtccgggtc gcccacgggc
     4261 ggcttggccg acgtcaggta ggggttgatg acgacgccac gctcggggat gccgtcgtcg
     4321 ttcgcgagct gccacgtgaa gatccgcagc cccacaccga tctggggagc cgccaacccg
     4381 actccgcggg cggcatccat cgtctcgaac atgtcggcca cgagggcacg gacctcgtcg
     4441 tcgatgacct cgacgggttg ggcacgctgg tgcaggactg gctcaccggt gacgacgacg
     4501 ggacggatcg gcatgccgtg attctctcag aggcgctgtc gggtgagcga gccggacacc
     4561 tgctcctgcg atcgtcgggt gaacccgtcg atcgtcatcg tcgcccccgg acacgccgnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gacggccgtc tggagcgcat
     4741 caccgttcgc gacacccgca gcggtgaaca ggaggtgctc ggctgccgtg gcctggtgct
     4801 gctgctcggg gccgagccgc actgcgagtg gctccccgct gaggtcgcga cggacggcag
     4861 ggggttcgtg ctcaccggac gcgacgtccc gaccgaccgg tggaccgacg gcatcccccc
     4921 tgcgagcctc gcgacgacgg tgcccggggt gatcgccgtc ggtgacatcc gggccacgtc
     4981 gatgaagcgg gtggcggccg ccagcggcga gggcgcctcc gtggtgccct tggtgcacgc
     5041 ctggctcgac cccgaccagg gctgaacccc tcaaggttcg gcggccggaa caccggtttg
     5101 tcgaaaactc tcccgttgca ggcgtccctg aacgtactgt gagcgcaccc cctctcgggg
     5161 tctgaactgt caccaagcga aaggaggtcg cgtgaacatc tttgacagcg ccaaggagaa
     5221 gctcgcggaa ctgaccgaga ctcacggcga ccaggtcgag agcatcagcg accaggccat
     5281 cgagaaggca ggcgacgcag ccgaccaggc aactggcggc cagttcgccg agcacgtcga
     5341 caccgcgcag tccgcggcag acggcgccat cggcacctga tctccccctg acgcgacccg
     5401 ggaccctcga ccgaacggtc gggggtctcg gccgttcggt cagggtttgc cggccgcgag
     5461 gcgaactgcc agcgcagcgt tggtcgcgtg cctacgctcg ggggaacccg cctcgtctcc
     5521 caggaagaga gccgtccatg gcgcacaaca cctcgggact gcccccgctc gacatcgagc
     5581 cgttcgaccc cgccccgctg tatgccgcag ccaactcacc cgagcgccgg gtggaccccg
     5641 ctcgggaacg cgcgcgctcg gtgcgagccc tggggctggt cctcgccctt gctgccgtgg
     5701 gcgtcgtcgc cgcggtcctc gcgctcgggt aggccgctca ggtcgcgcag ggtgcggtgg
     5761 ctgtcggtga cgtcgcgggc ttgccgagcg acggcatccc cagggagacg gccggcttgc
     5821 cgggtaccgg cgcatcctgc agcgaggccc acgcgtcgcc acccctggtg cgacgaaccg
     5881 agtaggcacc gccctgcacc ccgctgtcgg ccacgaggtg gtgcggcgcc ccgtaggtga
     5941 cccccacggt cacgacgtca cccgggcggg gacgctcagc gccgtcgggc acggcgaagt
     6001 gcacgaggcg gttgtcgcgg gcccggccgg acaggcgtgc ggtctcgccg tccttgcggc
     6061 cttcgccggg tgcgacgagc acctcgacct cacggccctc gagggtgcgg ttctcggccc
     6121 acgagacacg ttcctgcagc tcgatgagcc gctcgaagcg ctcctggacg acggccttgg
     6181 gtacttggtt cggcatcgtc gccgcgggcg taccggggcg gatggagtac tggaaggtga
     6241 aggcgctgga gaagcgggac gcctcgacga cgcgcagcgt ctcggcgaag tcggcctcgg
     6301 tctcaccggg gaagcccacg atgatgtcgg tggtgatcgc ggcatccggg atcgcggtgc
     6361 ggaccttgtc gatgatgccg aggaacttct cgctgcggta cgagcgacgc atggccttga
     6421 gcaccgtgtc ggacccggac tgcaacggca tgtgcaggct cggcatgacg ttgggggttg
     6481 cggccatggc ctcgatgacg tcgtcggtga acgcggccgg gtgggggctg gtgaagcgca
     6541 cccgctccag tccctcgatc tcgccacagg cgcgcagcag cttgccgaag gcgaaccggt
     6601 cgccgaactc gaccccgtag gtgttgacgt tctgcccgag cagggtgatc tcgatgacgc
     6661 cctgcgcgac gagagcctcg atctcggcga ggatctcgcc cggacggcgg tcggcctcct
     6721 tgccgcgaag gctggggacg atgcagaagg tgcaggtgtt gttgcacccg acgctgatcg
     6781 acacccagcc ggcgaaggcg gagtctcggc gggtcggcag cgtcgagggg aaggtctcga
     6841 gcgactcgag gatctcgacc tcggcgcgct tgttgtgccg cgcccggtcg agcagtgcgg
     6901 ggaggctgcc gatgttgtgg gtgccgaaga cgacgtccac ccagggagcg cgcttgacga
     6961 tgctctcgcg gtccttctgg gccatgcagc caccgacggc gatctgcagg tcggggttcg
     7021 cggtcttggc cgggcgcagc tggcccaggt tgccgtagag cttgttgtcg gcgttctcgc
     7081 gcacggcgca ggtgttgaac acgacgacgt ccgcggtctc cggacgctcg gccgcgggca
     7141 cgctcgcgag gtcgacgtag ccggcggtct cgagcaggcc ggcgaggcgc tcggagtcgt
     7201 ggacgttcat ctggcacccg tgggtgcgca cgtcataggt cttgggtgtc atggcgggtc
     7261 aagggtatgc cgggtgcccg ccaccgcggg aatctcgccg cggcatcagg gggttcacgg
     7321 cccaaatctg ccatttggcg ggtcggcgat gcccccggga ggccaaccgc gcgctccacc
     7381 gcgcccctga ccccccacct gaaccgctca atccaggcag gggggccaac cgcgcgctcc
     7441 accgcgcctc tggccccccg ccccggacaa cgccgatcga ccatcgccgc gagcgatata
     7501 ctcttgcaga gttctgcaac tctgcaagag agcgcgatga catgccggtt ccgctgtacc
     7561 aagccaaggc cgagttcttc cgaacgctcg gccacccggc ccgcatccgc gtcctcgagc
     7621 tgctggccga gcgcgaccgg gccgtccacg agctgctcgc cgcgatcaac gtcgaggccc
     7681 cgagcctgtc ccaccagctc gccgtcctgc gcctgggcgg cctggtcacc cagcgccgcg
     7741 agggtgggca ggtgatctac agcctcgccc tgcccgccgt gcgcgacctg ctcgcgagcg
     7801 cccgcgagat cctccagacc aagacggccg accagggtgg actcgtcgac gacatcacgc
     7861 cagagccccg ggcatgagca cggacacggc ccgccgggcc gtcatggagc ggctggatgc
     7921 cgtccgcccc cgcagccgcg acctgcgcgc catggtggcg acccctcgtc aggacgtcct
     7981 cgccggggtg accgtgggtg tcgtggccct gcccctggcc ctggccttcg gcatcgcctc
     8041 ggggctcggc gccaccgccg gcctggtgac ggcggtcgtc gccggcgtcg tggcggcgct
     8101 gttcggcggt agtcacgtgc aggtctccgg gcccaccggg gcgatgaccg tcgtcctcgt
     8161 gccgatcgtg gcgacccacg gagccgaggg cgtgctcgtc gttggcctca tggcgggcgt
     8221 gatcctcgtg gtgatgggcc tggccggtgc agggaggtac gtgcggttca tcccgctgcc
     8281 cgtcatcgag gggttcaccc tgggcatcgc ggccctcatc gccctccagc aggtgccctc
     8341 cgcgctcggc ctgtccgcgc gccccgagca cgtcgtcgtg tcggcggtgg ccgccgttct
     8401 cgagtggttc gggagccccg acctcgcacc ggtggccatc agcgtcgggg tcgccgcggc
     8461 gatcctcgtg ctctcccgcc tgcggccagc cgtacccgtg gcgctgctcg gggtggtggt
     8521 cgcgacggtc gccgtccgcc tggccgacgt gccggtcgag acgatcggcg ccctgcccga
     8581 tcggctgttc accctgacgg tgccgtcggt gtcctggagc gaccttcggg tgctcgccgt
     8641 accggccgtc gccgtcgccg ccctggccgc gctcgagagc ctgctctcgg caaccgtcgc
     8701 cgacgcgatg agcgtgtcct cgcggcacga ccccgaccgc gagctcttcg gccaggggct
     8761 ggcaaacctc gcctcaccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     8881 ctcaccgacg tcgtcgcctg gatcccgctg gccgccctgg cgggggtgct catcgccacg
     8941 gccgtgcgga tgatcgaggt gtcgagcctg cgcgccctgg cccgggcctc acgcggtgac
     9001 gccgccgtgc tggggctgac cttcctggcc accgtcgtgc tcgacctcgc gacggccgtc
     9061 gtgctgggca tcgtcaccgc cggcgggctg gccctgcgcc agctcgcccg gacgacggcg
     9121 ctgcacgagg agtccctccc ccagaagtac ctcgacgagg aggagcacgg cgcgcaggag
     9181 accgccctgc tgcgcgaacg ggtcgtcgcc taccggctcg agggcccgct gttcttcggc
     9241 ggggcccaca cggccctgct cgagctcacc gaggtgagcg acgtcaaggt cgtcatcctg
     9301 cggatgtccc acgtgagcac gctggacgcc accggggcgg ccgtgctcgc cgacaccatc
     9361 cgctcgctgg aggcacgagg ggtcacggtc ctgctgtcgg ggatgccgca acggttcgtc
     9421 ccacggttgg ccgcgaccgg catccaccaa cacctcgtca cgctgaacca cgtcttcgac
     9481 agcacacccg aggccatcgc gcacgcccgc cggcacgtgg cccgcgacca ccaccatgac
     9541 gagggcgtca ggccaggcgg gtgatcgcca ccacgacgcc cagggatgcc gtggcccgcc
     9601 ccgagtagat gcccttgaac ggggggacgt cctcgtagtc acggccgcgc gccacgacga
     9661 cgtggtcgac cccgacctcg gtgccgttcg tgggatcaac cggcgtccag ccaccgtccc
     9721 agtactcgac ccaggcatgg ctctcgccct tgctcgtctc accgacggcg gtgtccctca
     9781 ccggcacgag gtacccggag acgtaccgcg cgggcaggcc gaggctgcgc agggcaccga
     9841 cggtgatgtg ggcgaagtcc tggcagacac cggagcgctc ggcccaggcg tcctcgccgc
     9901 ggctgtgcac gctggtgctg cccggcacgt agctcatctc ggcacgcacc agggacgcga
     9961 gctcctcggc agccacacgg ggggtctcgc gtccgcgcac ggcatccacc aactcggcga
    10021 tgccgtcacc ggggcgggtg cgcgccgaca acatcagcca ctcgtagctg cggtcctgca
    10081 cggcagggtc gcgcacagcc gcccagtcga gcgacggggt gtgccgggcg acgtcggtgc
    10141 gctcgacggt ggagatggcc tcgatctcga gctgaccgtg gctggccagg ctctccatcg
    10201 ccatgacgtg ggtgccccag tggtcgcggt agacgtggct ccacgtcatc gggcggatgc
    10261 gaatgcggtt ctcgagggtg gtctggccgg cctcgttgac cggggtcatc cgcagctcgt
    10321 tgtgggcggc acgaacctgg ccgtcatagg tcagggaggt cgtgtggacg attcgccagc
    10381 ggcgcatgtc agaccacccc gtcccgggtc cacgtcgtga cctgtccgga ctggaagtac
    10441 cgggacgcca ccgcctgcga cgccgccatc accgacccct gcacccgcag catctgggcc
    10501 gggaggtcgg cgatcacgtc ggacgtcgac cggtactcca gcgacgtgcg gatgcggccc
    10561 agctgacgcc gggcctcgtc ggagatgccg atgcggtcct cctggggtga gagggcgtcg
    10621 agccggcgct cggcctcgga gagggcgaag aagaccgacc gggggaactc gcggtcgagc
    10681 acgaggaagg ccgctgcgcg gtcatcactg agcaggccgc gctggctgcg catgaaggcc
    10741 tgctgcgctc cacacgacga cagcaccacc gaccacgggg cgccgcctcg cggcagaccg
    10801 ccggtggcca cgagacgcgc cgtcatgtcg gcccgctcga ggctgcggcc gagcacgagg
    10861 aagtcccagg cctcgtcgtg cgacatcgtc gagtcggcga tgccggacac gagcgccgcg
    10921 cgttcacgca cccacgacag gtgccgctcg gtcgtgaccg tccgcccgaa gccgttccag
    10981 cggtgccacg tcgtgttgat gccctcccac agctcggtcg acaacgtctc gcgagctcgc
    11041 cgggcgttct cgcgggcggc gaagagggcc ccggtgatgg agctggcatt ggaccggtcg
    11101 aagaccaagg cggcacccac gtcggcgaac cccgggtcgc cctcgaacgg catccccatg
    11161 atcaccgaga ggacggtcct cgacgccgtg acctcgtcgg ccccggggtc ctcgaggagt
    11221 tgcaggcgca gcacgtcgac gatgcgcgcc gtgccgtcgg cgcgctcggt gtagcgcccg
    11281 atccagaaga gcgactcggc gatccggctc agcacgacga ctcctcgtgg cgggtgtcag
    11341 cgtgggccac cgtgcggtga ccttgctgct gttgctgctg ctgctgggcc ttcatgctgt
    11401 cctcgtggga tgccgtgggg gcactcatga cgacgacctc gcggttgtcc ggtggccggc
    11461 tggaggtgct ggtcgccgcc cgacggtccg agagcaccca ggtgtccttg gacccaccgc
    11521 cctggctgga gttgacgacc agctcaccct cgggaagggc gacccgggtg aggccccccg
    11581 gcagcacccg cacgcgctca ccgtcgttga cggcgaacgg ccgcaggtcg acgtggcgcg
    11641 gggcgaggcg gccgtcgatg aacgtcggca cggtcgagag ctgcacgacg ggttgggcga
    11701 tccagccccg cggatcggcg gtcagctggg cgcgcagccg ctcgagggtc tcggcgtcgg
    11761 ccttgggccc gacgaccagc cccttgcctc ccgagccgtc gacgggcttg acgaccatgt
    11821 cgtgcaggtt ctccaggacg tgctggagcg ccttcggctc gtcgcaacgg aaggtgtcga
    11881 cgttgccgag gatcgggtcc tcgtcgaggt agaagcggat gagctcgggt aggtaggagt
    11941 agatgagctt gtcgtcggcc accccgttgc cgatggcgtt gacgaccgtg acccgccccg
    12001 cccgcatgga ggagaccagc cccgcgacgc cgaggatgga gtcgccgcga aagtgcaccg
    12061 ggtcgaggaa ctcgtcgtcg atgcgccggt agatgacgtc gacgggccgg tctccggtcg
    12121 tcgtgcgcat ccgcacctgt ccgccgacgc acacgaggtc acggccctcg acgagctcga
    12181 cccccatcat ccgggccagc agggcgtgtt cgaagtaggc cgagttgaac accccggggg
    12241 tcagcacgac gacggtcggg tcgctggccc cgtcgggtgc cgcggcacgc agcgccgcga
    12301 ggagcatgcg cgggtagtcg tgcacgggcc ggatgcgcat cgtcgcgaac gcctccggga
    12361 agacgttggc catggcacgg cggttggcga tgacgtagct gaccccggag ggcacgcgca
    12421 cgttgtcctc gagcacccgg aaggtgccca cctcgtcgcg gatgaggtcg acgccggcga
    12481 cgtgcacgcg cacgccgttg ctcgggcgga tccccatgac ggcccggtgg tagtgcttcg
    12541 agctcgcgat gaggcgccac gggatgatgc cctcgtgcac ggcgcggggg atctcgccct
    12601 cgcgcgagta gatgtcatcg aggaaggact cgagcgccgt gacccgttgc accaccccgg
    12661 actcgatgat ctgccactcg tgggcgctga tgacgcgggg cacggcatcc agggggaatg
    12721 gccgctcctc ccccgcgtgg tcgaaggtca ccccctggtc gaggtaggag cgcgccagcg
    12781 agtcagaccg ctccttgagg gcctgcccgt tcatcccacg cagcgtgtgg aacaccggct
    12841 tgtagggcgt tcggggcgcg ccgggctcgc tgagcatctc gtcccacgcc tggccgaggg
    12901 cgtagtcgtc gaacaggccg cctggggcct cggccggcat ctgctcgctc acgtgacgaa
    12961 cctatggcca cggcgtgaac ctgtcatcac acccgtgtca cccccgtgtt tcgcgcatct
    13021 gttgtccacg gggtggacgg ccctagggtg gcgcgcatga cccagcccgg cccgacggtc
    13081 tccctgcgtc cggtggatgc cgggagctgg cgtgccgtcg ccgggctcac catcgcgccc
    13141 ggccaggacg ccttcgtcgc gactcccacc cactacctcg cgttgtgcgc ctacgaggac
    13201 gtctggaggc ccctggcggt gcacgacgag gagggcgcgg tgatcggctt cctcatgtgg
    13261 ggcatcgacg acgaggacgg cagctgctgg ctcggcgggg tgatcgtcga cgccgcgcac
    13321 cagggtcgcg gggtgggtcg ggccgcgatg gcccaggccc ttgcgaccct tcgtccccgc
    13381 gacgacgacg ctgggttcgc cctcgcgtac cacccgcaga acacggtggc gaagggtctc
    13441 tacgccagcc tggggttcgt cgagacgggc gagaccgacg acgacgaggt cgtggcgcgg
    13501 cgccgtcccg cctgaccctg gtgggtcggg tcgaccccag tcgccgcgcg acggtgcacg
    13561 cgcatgacag gctgggacac agttggctcg accccgaacc agcccacctc gagaggatcc
    13621 ttcgcgtgcg caccctgctc aacatcatct ggttcgtgtt cggcgggttc tggctcgccg
    13681 tcgggtacgc gttcgccggc ctcgtgctgt gcctgctcat cgtcacgatc ccgctcggcg
    13741 tcgccgcctt ccggatggcc agctacgcga tctggccctt cgggcgagcc gtcgtgcagc
    13801 agccggatgc cggcgccggc tcggtggtgc tgaacatcct gtggatcgtg ctcgcggggt
    13861 ggtggctcgc cctcgagcac gtcatcacgg cagtggcgca ggccatcacg atcatcggca
    13921 tcccgctggc catcgccaac ctcaagatga tccccatctc gctcttcccc tacggcaagc
    13981 agatcgtgcc gaccgactcg ctgtccgcag cgcaacgacc gctgcactcc ctctgagcat
    14041 ccgccgcagg gagtgcgggc acccgtgacg gggttgtctg ctcgtgacgg gggtagaggc
    14101 ccgtcacgag cggacaaccc cgtcaccggt cggtcaccgt ggccgtcaga acgtgccgcg
    14161 gtgccttcgc gcctgagccg ccgatttggt cacgtcgacg ccataggcat cgcgtcctgt
    14221 cgacgcgaat gcggacagcc ggtccccggc ccgttcgaag agccgctgcg ccccgacctg
    14281 gttccctcgg gcggcatggg tcagggccac gcagacctgc gccagtccct gccacagatc
    14341 cgcctcctcg gccggcccgg ccttccaccg cgcctccagc acttcgtggg ctgcgaaggg
    14401 acggccggca tcgatgagct ccctggcctt cctgagcgtt tcgtgcggcg gcagcggttc
    14461 ctccgacacc ggctcgacac cctcggcccc gtagggcagc gggcgaccca acgcgtctcg
    14521 cggccgtgcc tgacgcgcac gtccctcggt gtcccggtcc cgctctgctc cagtcacacg
    14581 agaagcgtcc cagagaagtc gagcccggcg cgcgacgcgg tggtcacgtt tcacgtctgc
    14641 ctccacgcgc caccgcgaac tcgtgctcaa gcacggccat caccacatcg tcataccagc
    14701 ggccgtcaag gaagcaggtt tcccgctcgc gtccctcctc cacgaagccg catgcccggt
    14761 aggtggcaat cgctcggtgg ttgaagtcca ggacgcgaag atccacgcgg tgcagcatca
    14821 acgtctcgaa ggcgtgctgg agaaccaggc gcgtcgcctg ccggccaagc cctcggccga
    14881 agaacgaggg gtcgtagagc ccgatggcat atcgggcnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnggc tgctctcggg ctcgctgtgc tggctggtgg cggccaggtg
    15061 gcccatccgg cgcagtacgg tcacgtgaac acacgctgag cacctcacgg gagaggaccg
    15121 ggatgagacc acttcgatac tcgatcaacg tcaccctcga cggctgctgc caccacgagg
    15181 cagggctccc gcccgacgag gagtcgatgc gctactggac cgctgagatg caacgagccg
    15241 atgccctggt cttcggccgg gtgacctacg agatgatgga gtcggcgtgg cgaaagccgg
    15301 ccacgggcac ctggcctgac tggatgggcg agcgggagat cccgttcgcc gagaccatcg
    15361 accgggcaaa gaagtacgtc gtgtcgagca cgctgagcga ggttgactgg aatgccgagc
    15421 tggtgcgggg cgacctgggg cacgccgtac agcagctcaa gcacgagccg ggcgagggcc
    15481 tcttcgtggg tggggtcacg ctccccctgg cactggccga cctgggactg atcgacgagt
    15541 acgcgttcct ggtgcagccg gtccttgccg gacacgggcc gacgttgctc gccggtctgc
    15601 gcgagcgcat ccagctcgag ctcgtggacc gccaggagtt ccgttcggga gcggtcgccc
    15661 tgcgataccg gcccacctga gccccgaggc cgacgatcac tcagtcgcgc tggttctcgg
    15721 gcgcctcgcg caccgcctcg cgcacgatgc gcatcgacac gtccgaggga taacccttgc
    15781 gagcgagcat ccccgcgagc cgccgggtct gcacgagggg cgtcaacccg cttatcgagc
    15841 gcatcttctt cgcgacgagc gctcgggccc ggtcctcctc gtcgtgcggg tcgacctcgt
    15901 cgagcgcggc ctgcgccgtc tcgtcgtcga cccccttgcg tcgcagctcc tgcgcgaggg
    15961 cccgccgggc gagcccgcgg ccggcctgct gcgagcggac gagcatcccc gcgtaggcgg
    16021 tgtcgtcgac gagcccgacc tcctccatcc ggtcgagcac cgcagcggcg atctcgtcgg
    16081 ggcagtcctt cttgcgcagc gcctgttcga gctgggcgcg gctgcgagga gccgcggtga
    16141 gctgtctcag gacgatctgg cgcgcccagt cgtatggatt gggctcgctc ccccgcgtgc
    16201 gcgggtcagg cggcggcggc gagcccacca ccggctcccc cgccgtcggc gaccccaccg
    16261 cccaggaggg cggcggggtc gtcgagtcag cgaacggcat cccgaatcgg gggtggctca
    16321 gaagtcgacc gggatgtccg ggacggcggc cagggcgtcg acccgcggca ccccgatgcc
    16381 gagcttgtcc ttgatgttct tctcgagctc gtcggcgagg tcggggttgt ccttgaggaa
    16441 ggtccgggcg ttctccttgc cctgccccag ctggtcgccg tccatcgtgt accaggcgcc
    16501 ggccttgcgc acgaagccct gctcgacccc catgtcgatg aggccgccct cgcgcgagat
    16561 gccctgcccg tagatgatgt cgaactcggc ctgcttgaac ggcggactca ccttgttctt
    16621 cacgaccttg cagcgggtgc ggttgccgac cgcgtcggtg ccgtccttga gggtctcgat
    16681 gcgccgcacg tccaggcgca ccgacgcata gaacttcagc gccttgccac ccgtggtggt
    16741 ctcgggcgat ccgaacatca cgccgatctt ctcgcggagc tggttgatga agatcgccgt
    16801 cgtgcccgtg gtgctgaggg caccggtgat cttgcgcagc gcctgcgaca tgaggcgggc
    16861 ctggaggccg acgtggctgt cgcccatctc gccctcgatc tcggcacggg ggacgagcgc
    16921 cgcgacggag tcgatgacga tgatgtcgag cgcgccggag cggatgagca tgtcggcgat
    16981 ctcgagcgcc tgctcccccg tgtccggctg ggagacgagc aacgcgtcgg tgtcgacacc
    17041 cagggccttt gcgtactcgg ggtcgagcgc gtgctcggca tcgatgaagg ccgcgatgcc
    17101 gccggccttc tgggcgttgg ccaccgcgtg cagggcgacg gtggtctttc ccgagctctc
    17161 cgggccgtag atctcgacga cgcggccgcg gggcaggccg ccgatcccga gcgcgacgtc
    17221 gagggcgatg gagcccgtgg ggatgacctc gatggggggc cgcacgtcgt ctccgaggcg
    17281 catgacggca cccttgccgt gctgcttctc gatctgcgcc atcaccgagg cgatggcctt
    17341 gtccttctcc ccaccgttgg ccgcagccgt cgggttgggc atcacgggtc cgcgatttcc
    17401 tgccatgtca caccgtctct tccagttcgt gcagcgagcg cgcccaccgg cgaccagctc
    17461 tcgccatcgg tctcgtcgcg aggtctgtgt cagacgctag gtggcggtac cgacaacggt
    17521 cacgggctgc cgtcgagcct gtggacaagt gccgtcgggg acaccacctg tggatgacca
    17581 tagacgaaca cctgttcgat cccacacatt ggcgggcgac acgccgatgt cgtgtggtcg
    17641 aatcagccgt gcccaccgca gacggccatc accggccact caacgcccgg aggcccgtga
    17701 ccgtcgcgac gacgtttccc gggatcacgg tgtggcgcac gtaccagcgc ggctggctgc
    17761 gcggcgacct gctcgccggg gtgaccgtcg ccgcctacct catcccgcag gtgatggcct
    17821 acgcccagat cgcggggctc ccgccggccg caggcctgct cgcgatcatc gcgcccaccc
    17881 tcgtctacgc cgtcctcggc tcctcccgcc agctctccgt cggccccgag tccacgacgg
    17941 cgctgatgac cgccaccgcc gtggccgcgg tcggtgtcag cggccccgac gcgtacgccg
    18001 cactcgccgc ggccctcgcc gtcgtcaccg gcgccctgtg cctcctcggt tgggccttcg
    18061 gactggggtt cctcgccgac ctgttctcca aaccggtgct cgtcggttac ctcgccgggg
    18121 tcgccgtgct catggtcgtc agccagatcg acagcctcac cggcatcgag gtgagcggcg
    18181 actcgatgcc cgccgaggtc tggagcgccc tcacccacct ctcggacgtc cacgcaccca
    18241 ccctcgtgct ctcgagcacc ctcctcgtgc tgctcctgct cagtgcgcac ttcttccccc
    18301 gcctgcccaa ccccctgctc gcggcactgc tcggcgccgc ggccgtcatg gtgctgggcc
    18361 tcgacgagct cggtgtggcg gtcgtcggcc ccctgccgtc ggctctcccc cagctgagcc
    18421 tgccggacct gtcgccggat gccgtggcca ccatgctggc gcccgcgctc ggcatcgccg
    18481 tcgtcgccta ctccgacaac gtgctgaccg cgcgagcctt cgccgagcgc tccggtcagc
    18541 agatcgacgc caaccaggag ttcctcgcgc tcggcgcggc caacatcgcc ggcgggttgc
    18601 tctccgggtt ccccgtgagc agcagcggaa gccgcacggc gatcggtgcc tcgatgggca
    18661 gccggaccca gctgtactcg ctggccaccc tggccaccgt gctcgtggcc gtgctcgcgc
    18721 tcggcccggt catcgccgcg ttcccgaagg ccgccctcgg ggccctcgtc gtctacgccg
    18781 cgatccggct cgtcgacgtc gccgagatcc ggcgagtggc ccggttccgc cgcagcgagc
    18841 tcgtcctgct cgtcgcgacc acgctcgggg tgttcgtgtt cggggcgctc tacggcatcc
    18901 tgctcgccgt cgcgctctcc ctcggcgacc tgctgcgccg cgtcgcccgt ccgcacgacg
    18961 gcatcctggg ctacgtgcgc ggcatggcgg gcatgcacga cgtcgacgac ttcccggatg
    19021 ccgtgcaggt gccgggcctg gtggtctacc gctacgactc gccgctcttc ttcgccaacg
    19081 ccgaggactt ccacacccgt gcgctggccg ccgtggacga cgccgacgac ccggtcgagt
    19141 ggctcatcat caacgccgag gccaacgtcg aggtcgacct gacgagcctg gacgccctgg
    19201 ccaggctgca cgacgacctt gtcgcccgcg gcgtcgtgct cgccatggcg cgcgtcaaac
    19261 aggacctgct cgacgacctc gaggcggccg ggctggacca cgccatcggc accgagcact
    19321 tctacccgac gctgccgacg gccgtcacgg cctacctcgc ctggcacgcc gagacccacg
    19381 gcaccccgca cccgttcggc caggtgccct agggtccacc cgcgtcaggt ggcccgggtc
    19441 gccacctcca gcagctcgag cacgtgctgg tactgctcgc cggtcgcgcg cgtcatgccc
    19501 agctcgcacg tgcggttgca cgaggcgtag gcgtcgaacg gcccggcctc cgcgatctgc
    19561 gcggcctgca cctgcgtggc ggaggccgtc agctccgggt gcagcagccc acggtccccg
    19621 gcgaagccgc agcagcccca ggagtcgggc acgtagacct cgtccgccac cgctcgcgcc
    19681 accgccatca gcgcagggtc gagccccagc tgggtcgatg agcacgtcgg gtgcagcgcc
    19741 agccgctgga gcctgcgcgg cgccggaagc tcggtcagca ggtcgggcag cagctcgccc
    19801 gccacgtagg ccacggcatc gcggacacgg atgccggcgt accgggcgtc cgtctcggcc
    19861 gcgtgggaga gcgtggtcac caggccctcg gtgcacgagg ccgcgtcact gaccacgtcc
    19921 agctcaccgc cacgcgtggc ctcccacagg gatgcggcca cccgctgctc catggcctgc
    19981 gcgccctcga gcatgccctt ggagcgccac ggggtgccac agcacaggcc ggggaggttc
    20041 ccgggcgcca cgagaacgac accagcacgt cggcacaggg acaggaaggc gccccgcact
    20101 ccgggtccgg cgtcgctggg gccgaagagg gttcccacac atgccgagaa gtacacggcc
    20161 tcgcgcgggc cctccccccg ctcgctcacc gcgggggccc agggagccct tccctccgga
    20221 cgctgtcgtc cgccaccggg cagctccggg gaccacaacg gcaccacatc gtcgtcaccc
    20281 gccctgcgca cgagagcgtt cgggacgagc accacgggat ccggcagcac tcgcgccatc
    20341 gtcagcgccg ccgaggccac ccgtgtcgtg gcgccccagt gtccggccgc caggcgccag
    20401 ccacggctcg ccatcgggcc caccgcctct gcccgcagcc gcttggtgag gtcaccggtg
    20461 ttgatgtcga cggggcaggc ggtctggcac atgccgtcca ccgcgcaggt ctcgacggcg
    20521 tcgtactcgt agtcgtcctc gagctcgtgc agcagcgcca ggtctcccgc agctgcagct
    20581 gcctgcatct cacggcgcag cacgatccgc tgcctcggcg tcgtggtcag gtcgcggctc
    20641 gggcacaccg gctcgcagta cccgcactcc acgcaccggt cgacctcggc ctcgacggtc
    20701 ggcgtcacct tgatgtcgcg ggtgtgggac tcgcggtcat cgctgatgat cacgccgggg
    20761 ttcagcacgc cgcgcgggtc gagcagccgt ttgacctccc tcatgacgtc gtgcagctcc
    20821 tcgccgtact gccgccgcac gaagggcgcc atcatccgcc cggtaccgtg ctcggccttg
    20881 agcgacccat cgaaccccag caccagatcg accatctcgt cggtgaacgc catcagccgg
    20941 tccatccgac cgccctcgag ccgctcgttg agcatgaagt ggatgttgcc gtccttggcg
    21001 tggccgaaga tgacgttgtc ctcgtagccg tgcttcgcga agagctcgat gagccgctcg
    21061 caggtcggca gcagggccgg caccggcacc acgatgtcct cgagcaacgc cgtggtgccg
    21121 ctcgggcgcg cgccggccac cgcggcatac aggcctttgc gggtggtcca gagccgcgcg
    21181 cgcgacgcgg ggtccgaggc cagggccgcg ggcgagctga gccgcagccg ggccagcgcc
    21241 gggagcgcct cccggcccat tgccgtcacc tcgtcagcgg tgctcgcctg gtactccacg
    21301 agcagcgccg cgtgccggtc cacggcaatg cgcttgaggg cctcatcggc cctggggtcg
    21361 gcctggccga ccctcaagga cacggcatcc atcagctcga tcgtcgccgg ggcggtctcg
    21421 aggatggccg acagggagcc ggtcgcgccg gcgaggtcgt cgaagacgag cagcccggtc
    21481 atcgcgtggg gcagcagcgg caccgttcgc atcaccacgg atgccacgaa ggccagcgtc
    21541 ccctcgctcc cgacgaccag gtgcgcgagc acgtcgaccg gccgggtgtg gtcgaggaag
    21601 gagttcaggc cgtagcccat cgtgttcttc atcgagaact gctgctcgag caggcgcacg
    21661 gatgccgggt tgccccgcac gcggtcccgc agccggtcca gccccgcgta cagctcgggc
    21721 tcgagctggc gcagccgttc gtcggcgtcg ggggcgccgg tgtcgagcac ggtgccgctg
    21781 ggcagcacga gcaccagcga gtcaagggtg ttgtaggtgt tggccacggt gccgcaggcc
    21841 atgccgctgc tgttgttgtt gacgaccccg ccgagggtgc acgcgatctc gctggccggg
    21901 tccgggccca gcttgcggcc gtgacgtgcc agccgggcgt tgaccgcgcg cacggtggca
    21961 cccggccccg cgcgcacgcg caggccgtcg tcgaggacgt cgatgctgcg gaagtgccgg
    22021 cgggtgtcga cgaggatgcc gtccgtgccg gcctgaccgg acaggctggt gcccccggac
    22081 cggaacgtca tggggatgcc gttcgcggcc gacaccgcga acagggcgcc gacctcggag
    22141 ccgtcaacgg gcacggcgac cgcctgcggg agcagggcgt agtgcgacgc gtcgtgcatc
    22201 atcttcagcc ggtcactcat ccgggagtgc aggcggtcag gatccccgag tgccgcggcg
    22261 aaggggttca cgagtcggct cccgcgacga gcacgacgac cagcgtgcgg gggccgtgca
    22321 cgccctcgac ccgctcgagc tcgatgtcgc tcgtcgcgct gggtccgctg atccaggtga
    22381 gcggccgggc accgtcgggt ccgggccgca gcctggccac cgactcggga acgtcgtgca
    22441 cgacctggtc ggcccggatg acgcacacgt gggtgtccgg cacgagcgt
//
LOCUS       DAOSOC010000025        20837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_2547_length_20837_cov_10.326244, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000025 DAOSOC010000000
VERSION     DAOSOC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 20837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20837
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2547_length_20837_cov_10.326244"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PL014_03655"
     CDS             complement(<1..608)
                     /locus_tag="PL014_03655"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT56216.1"
                     /translation="MTSGRVVEHPVSRDPGLAARAQAWLAAAERSVAVPRLAATVMLV
                     RDGAAGPQVYVQRRVASMAFAPSTVVFPGGGVDPVDHALDPATPGLADLAAVMGVAAD
                     VAAPFAAAAVREVQEECGVVITVADLRGRAHWVTPEFEPRRYDTWILAAGMPADQEAR
                     GTTTESDHSAWVRPGDLLVRHRAGKVRMLPPTVISLEELADFA"
     gene            complement(631..1104)
                     /locus_tag="PL014_03660"
     CDS             complement(631..1104)
                     /locus_tag="PL014_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT56217.1"
                     /translation="MGAVESRLAELGLVLPEIVPPLAAYVPAVLDGSRVYVSGQVPMV
                     GGVMAATGAVGEGEGFVSPERAKELAAVCALNALAAVKSVVGDLDRVERVVKVVGFVA
                     SDPSFTGQPGVINGASELFGDVFGDAGRHARSAVGVVALPLGAPVEVEVIVHVRD"
     gene            complement(1106..1258)
                     /locus_tag="PL014_03665"
     CDS             complement(1106..1258)
                     /locus_tag="PL014_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56218.1"
                     /translation="MTTYEYATVPLIVHATKQILDQWGADGWDLVQVLVMPEGNLVAY
                     LKRPKA"
     gene            1305..2063
                     /locus_tag="PL014_03670"
     CDS             1305..2063
                     /locus_tag="PL014_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT56219.1"
                     /translation="MSERPADRPADRTEITGPHQSDVYDVWEPAPHRARGVSVALIHG
                     GFWRERYDRHHLAPLARALADDGFHVANLEYARAGMPGGGWPGTGTSVLAQLTAAHAD
                     PALPERVVAVGHSAGGHLALWVASADRAPWLTGAVALAPAADLGEVDRLGLSDHAARN
                     LIGAAPDDAPDTWADADPARQRLTTPAVIVSGEHDDDVPASVIESYLATRTPDDPIHS
                     AVALGADHFAVIDPQAPAYLLVLAEIEELTLATH"
     gene            2118..3131
                     /locus_tag="PL014_03675"
     CDS             2118..3131
                     /locus_tag="PL014_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOT56220.1"
                     /translation="MSTPSLARAFDGVRLHIVTGKGGVGKTTVAASLALSLASQGKRV
                     LLVEVEGRQGISQTFDVPPLGNDEVRLVTHAGGGELWGLSVDAKAALMEYLQMFYKLG
                     RAGGALEKMGVVDFATTIAPGVRDVLLTGKVYEAVGRTTGNRRGQGTKVWDAVVLDAP
                     PTGRITRFLDVTAQVADLARIGPIHSQAESITRMLRHRESVVHIVTLLEEMPVQESVD
                     AVTELSAAGFGVGTVIVNLVREPIVDEALLEVAAAEPARVADQVRADLVSVGVKTSKA
                     TVTGLLSQAHDHAERVALERDLAAEVAAQGLPTLRLPALPTGVEDGGITVLADALTAQ
                     GVR"
     gene            3131..4285
                     /locus_tag="PL014_03680"
     CDS             3131..4285
                     /locus_tag="PL014_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOT56221.1"
                     /translation="MARRPAPARLDVDALLADPDTGIIVCCGSGGVGKTTTAAALGVR
                     AAESGRRVVVLTIDPARRLAQSLGLTELDNVPRPVKGVGEEAGGSLDAMMLDMKRTFD
                     EVVEAHSTPDKAAQILANPFYQAVSSSFAGTQEYMAMEKLSQLRAQADREGTWDLVIV
                     DTPPSRSALDFLDAPKRLGSFLDGRFIRLLTAPARAGGRAYLKVFSVSATIAAATISK
                     VLGGAFLSDVQTFVAALDTMFGGFRERADLTYALLKEPTTAFVVVAAPERDALREAAY
                     FVERLESEGMPLAGVVVNRMQALAAPSLSGGRATAAAEQLEDAGGSELTPALLRLHAD
                     LSSIAERHEGHVRRFVAGHPGVPVSTVPASATDIHDLDGLRTVGAALAAT"
     gene            complement(4315..4638)
                     /locus_tag="PL014_03685"
     CDS             complement(4315..4638)
                     /locus_tag="PL014_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT56222.1"
                     /translation="MSAAPEVAPALGDWVADWVGLGHCADEDPDALFVQGKAQRAAKV
                     MCKGCPVMAECLADALDNRTEFGVWGGMTERERRALLRRRPEVQSWSGLLAQVKGAAV
                     HGESA"
     gene            4893..7298
                     /locus_tag="PL014_03690"
     CDS             4893..7298
                     /locus_tag="PL014_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOT56223.1"
                     /translation="MDGRAHNVSAVLRLLLGFVVLSTIAGLLMAGLAIPAVGATGQMA
                     KGGVGVFNDLPSEFTVSPLAQQSRILNADGKVIANPYDENRIIVPLAKIAKNMQNAQL
                     AIEDSRFYEHNGLDVRGFSRAMVSNLQGGDTQGASTLTQQYVKISLQYRALSNGDKDA
                     AAAAVEKSYSRKLQELKYALNVEENFTKEQILEGYLNLVYYGDQAYGVEAAAQNYFGV
                     SAAKLNLGQAALLAGIVQQPTAFNPVLHPKAAQSRRNQVLDRMQALGLASAKDVAAAK
                     KQEVAKVVKRKPAKGVCFRSPEPYFCAYVMEWLQKSPQMAVLGKTPAERLKTINQGGL
                     VIRTTLNPAMQASAKKELAKAVKPNNKQNLAGAVTMIEPGTGKVLAMVQATDFAKYQT
                     NINVDQEYGGGPNGYQIGSTAKTFALVEALERGMPLNATINVPASSPTKPAVFEPKNM
                     VDECSTNEPWPVKNDFSMSAGPMTLREGTVKSVNPFFAALNVRLGACSVRDTMVKMGL
                     HRADGKPISDTISGIALGAGESTPMSVASAYATLAANGKYCEPQPVTAISTPDRKSLK
                     IPAAKCSQVISADVAAGVTDLLQSVVKVNLGSMWSSSARPAAGKTGTTERFNQVWFAG
                     YTPQISTVVLVGNLKPANKNGKLYTLRGKCFGDYGCPSRVYGSTVSAPIWSKIMKTAH
                     KGMPVKDFKSPSADIRKGNYVRLPNVIGRGPDSALARLKEAGFSGFVAGRTNSSLPAG
                     TVAFTDPSGSALPGGRVGLWLSNGYTPPPTAPKKAAAPEKPQQTPEPAKKPGKPAPKP
                     TKS"
     gene            complement(7367..7831)
                     /locus_tag="PL014_03695"
     CDS             complement(7367..7831)
                     /locus_tag="PL014_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOT56224.1"
                     /translation="MTDDTLKARLQHDLHDAMRARDTVRSGTLRMTLTAITNAEVAGD
                     EARELSDDEVLKVVAKEAKKRKEAAAAFTGAGRPELAATEEAELLVLEGYLPAQLDDE
                     ALREIVDRAVAATGATGMPQLGMVMKAAQGEVAGRADGGRVAAVVKQVLSGA"
     gene            7890..8858
                     /locus_tag="PL014_03700"
     CDS             7890..8858
                     /locus_tag="PL014_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT56225.1"
                     /translation="MDDLARTALRTAGGLAGAGLVGIAYAAFVERTWFTLRRFAVPAL
                     PPGAAPVRILQVSDLHLTPGQTKKIDWVRSLAELEPDFVVNSGDNLAHLDAVPPLLRA
                     MEPLLERPGAFVLGSNDYFAPTPKNPARYLTSRHADAPARRSTLPVKALRDGLGSKGW
                     ADLNNARRIVRMGDHDVELVGVNDPHIGYDRYAKVAGPASTDVALTMGLVHAPYQRVL
                     DAMVGDGASVVLAGHTHGGQLAVPLWGALVTNCDLDTRRAKGVSRWWPGAGTAGARGG
                     AAPSSDAPEDAAWLHVSAGLGTSPYAPVRFACRPEATLLTLLARDS"
     gene            8933..9009
                     /locus_tag="PL014_03705"
     tRNA            8933..9009
                     /locus_tag="PL014_03705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8967..8969,aa:Pro,seq:cgg)
     gene            complement(9149..9796)
                     /locus_tag="PL014_03710"
     CDS             complement(9149..9796)
                     /locus_tag="PL014_03710"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HOT56226.1"
                     /translation="MVADLVPLVIGILASPFPVIPVILLLLTPRATANAVSFLGGWAA
                     GVAAGTALFVGLATVIDGFEETPTWVSWARVLLGGVLVVVGVRAFLTRHANAEPPGWM
                     RALGESTPPSALRLGALLSGANPKILLLSAAAGLTIAAEEQGTAATVATVIAFALVGS
                     LSVALPIGVHLVLGDRAQAPLRRAEAWLTTHNATIMAVVVLVIGALLLSKGLSGI"
     gene            complement(9796..10848)
                     /gene="glsA"
                     /locus_tag="PL014_03715"
     CDS             complement(9796..10848)
                     /gene="glsA"
                     /locus_tag="PL014_03715"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HOT56227.1"
                     /translation="MTDPGSSLPGRGATADLGATYVSTGHLPRDEWVREAVAACHAEV
                     ATVDAGRVSTVYPALAQADPSLFGIAVIGVDGHEFVAGDVDVPFALMSVAKPFVFALA
                     CARLGVDGVVDHIGVDATGRPFNSLAAVEHSPDGRTNPMVNSGAIACTGLFLGRNRDF
                     TVDAAWDELRAGLSAFAGRDLSLDDGIHDSARATNQRNRALGQLLHSLGRLDDPDGAV
                     DLYTRQSSLAVTARDLAVMGATLADGGVNPVTGVAVVSPQVAARTLAVMTTAGMYETS
                     GEWLVRVGLPGKSGIGGGIVTAAPGKGGLGTFSPPLDAAGNSVRGILAAERLSRTLGL
                     DLFGSAALVPVVRSRR"
     gene            complement(10875..12593)
                     /locus_tag="PL014_03720"
     CDS             complement(10875..12593)
                     /locus_tag="PL014_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HOT56228.1"
                     /translation="MIPDLFLTPDERENPDTRIDEGKPPGVAWTEGNHVRPIVHGAPY
                     LAELHERIEALGDGDLLYFVDWRGDPDQLLTDDPGSGVSATFAAAARRGVEVRGLLWR
                     SHWHRFGFHSQKSRLLGIEIDEAGGQCLRDMRVRTGGSHHQKFVLLRHRDEPSRDIAY
                     LGGIDLCHGRRDSIAHEGDPQRIAMPQVFGARPPWHDVQVAIQGPAVADVETTFRERW
                     EDSTPLTLNPGRLASSLLQGEDLQPQPLSPQGPPPPKVGEMTSGGGATASEAGATASE
                     AVQIVRTYPSILPKGYDFAPRGERSIAHANTKAVAHARHLIYLEDQYLWSQEVGRHFG
                     EALRANPQLRLIAVIPMVPDVDGAVSLPPQLYGRKLAMDLLLEAGGDRVAVYGLTSDA
                     GYPIYVHAKVCVIDDVWSSVGSDNFNRRSWSSDSEIACAVQDTRVRGLDEPAPRDSFA
                     RRLRRELVGEHTGLDPEDVPDDADELWDLMAQRADALDEWYASGAGPRGRRHLPRPKG
                     PMKGTSVRWRRRSGAWSGADGVERPPGRLRRLSPPELSRAQMLWAPRLYDAFDPDGTI
                     LRDDAI"
     gene            complement(12622..12852)
                     /locus_tag="PL014_03725"
     CDS             complement(12622..12852)
                     /locus_tag="PL014_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56229.1"
                     /translation="MSQYVGVDAWWVAVVIYAVLLVGQRLWRARSWVPPEWPEADYGW
                     KVTHLFWVPFHVLDVVFGVPLIFAIVIAYFAI"
     gene            complement(12867..14066)
                     /locus_tag="PL014_03730"
     CDS             complement(12867..14066)
                     /locus_tag="PL014_03730"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOT56230.1"
                     /translation="MTVRTVAMLTAGGLAPCLSSAVGGLIERYGEVAPDVRIIAYLGG
                     YAGLLRGEWVEVTPEVRENAHLLHLRGGSPIGNSRVKLTNVADCVKRGLVQEGQDPLH
                     VAAEQLTKDGVDVLHTIGGDDTNTTAADLAAYLHENDYELTVVGLPKTIDNDVVPIRQ
                     SLGAWTAAEQGSIYAQNIIAEHSSNPRMLIVHEVMGRNCGWLTAETARRYHAWVAEQQ
                     FVEGFGNDPRCWDVHAVFVPEEHIDIAAEAERLKAIMDEIGCVNIFLSEGAGVAEVVE
                     ELKAAGQEVPVDPFGHVQLDKVNPGAWFAKQFGAKLQAEKTMVQKSGYFSRSAAANDE
                     DLALIAQCTSLAVDSALAGVSGVVGHDEEQGDTLRAIEFPRIQGHKKFDVTVPWFREL
                     KESLGQA"
     gene            complement(14063..15241)
                     /locus_tag="PL014_03735"
     CDS             complement(14063..15241)
                     /locus_tag="PL014_03735"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT56231.1"
                     /translation="MGTNQARPQGWQPTLAVVGATGAVGRVVLDVLPTRHSHWAQVRL
                     AAGSEDVGTVLRVAGKDAVVEALTPQFFDGVDVAIFDIPPGIAREWVELAAARGVVAI
                     DNSTVFRTEHDVPLVVPEVNPQRVADRPRGIIANPGATVMTMIDALAVLHAAWELTEL
                     VITTLQAASGLGRAGISRLHDELDVVHGQRELGTRPGDVRRLVEHELGESVFPAPLVL
                     NVIPFVGHHVGDGWTSEEVKVRDETRKILGMPDLPITTTCIRVPVVSSHSVIVHATFA
                     RKVRVNEARQALVEAPTIVVLDDPDEPEFPTPNDVVGADPRFAGRIRQAPDAPMTLDL
                     FISGDNLRKGAALNMVQTAELVARDLVETTTMDAVAPASSSRTIEHPRPPAPDIRSTP
                     "
     gene            complement(15243..16526)
                     /locus_tag="PL014_03740"
     CDS             complement(15243..16526)
                     /locus_tag="PL014_03740"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT56232.1"
                     /translation="MPIVVQKYGGSSLADAESIKRVARRIAETRKAGNDVVVAVSAMG
                     DSTDDLLDLAQEVSPVPPPRELDMLMTAGERISMALVAMAISDLGYSARSFTGSQAGV
                     ITDGVHGKAKIIDVTPGRITEALAKGHIVIVAGFQGVSQGTKEITTLGRGGTDTTAVA
                     LAAALRADVCEIYTDVDGVFTADPRIVPSARKLDRISNEEMLELAASGSKVLHLRSVE
                     YARRFDIPIHVRSSFSHKEGTIVTDHPAPEGPEGDAVEAPIIAGVAHDRSEAKVTVVG
                     VPDRTGMAAKIFEAVTLAQINIDMIVQNVSATATGLTDISFTLPKTDGTAAVEALQRV
                     QADVGFSGVQFDDQIGKLSLVGAGMRSNPGVSATFFKALAEAGVNIEMISTSEIRISV
                     ITRGDLLDDAVRAVHTAFGLDSSQGEAVVYGGTGR"
     gene            16694..17818
                     /locus_tag="PL014_03745"
     CDS             16694..17818
                     /locus_tag="PL014_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT56233.1"
                     /translation="MSIVIIGAGLAGGTAATELREQGYEGTVVLVGAETHPPYERPPL
                     SKGYLLGNDSLEDAFVHPRDWYAEHEVDLRLGVEATGLDTDAHVVSTTGGDLTYERLL
                     LATGAEPRRLAVAEDSGVPTAYLRTIEDSDRLRDAFAAGGRIAVVGAGWIGLEVAAAA
                     REKGCEVTVFETAELPLLRVLGPTVAQVFADLHREHGVDLRLGTSVAADDLAGADLVV
                     AGVGVSPRLDLARSGGLAVDDGVLVDAGLRTSVASVYAVGDIASHAHPVLGRRIRVEH
                     WDTAINQAKVAAHNLAGADETYDAMPYFFTDQYDLGMEYVGHASGDDEVVVHGDLSAR
                     QFRAFWTREGRVVAAMHANDWDATDDLRAAVESGELPASP"
     gene            complement(17842..18426)
                     /locus_tag="PL014_03750"
     CDS             complement(17842..18426)
                     /locus_tag="PL014_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOT56234.1"
                     /translation="MSEFSVASASASDDWGQIAAESAADAQMYLVTVREIAAGNSPDA
                     TLPMALLALSQVLVMGARLGAVEDVVPAERFEPDAGPDAEFDPLRAGLANVFEGLDEY
                     GDVVDPVLDPEPVVGYLSNDLVDIASALSHGLSHYEEGRTLEALWWWQFSYLSHWGER
                     AAAALRVVQTLLAHVRLDADPDAVSEAQFDALHP"
     gene            complement(18419..19018)
                     /gene="recR"
                     /locus_tag="PL014_03755"
     CDS             complement(18419..19018)
                     /gene="recR"
                     /locus_tag="PL014_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT56235.1"
                     /translation="MYEGAVQDLIDELGRLPGVGPKSAQRIAFHLLQSDAADVERLAH
                     ALTEVKAKVRFCQTCFNIAEAQQCRICLDPRRDVHSICVVEEPKDVVAVERTREFRGR
                     YHVLGGAISPIDGIGPGDLRVRELMTRLGDGTVTEVIIATDPNLEGEATASYLSRMLK
                     DFGLRVTRLASGLPVGGDLEYADEVTLGRAFEGRRLIDV"
     gene            complement(19086..19337)
                     /locus_tag="PL014_03760"
     CDS             complement(19086..19337)
                     /locus_tag="PL014_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOT56236.1"
                     /translation="MTTDDTSNTAASTQTFRATGLTCGHCAHAVTDELSALDGVDGVE
                     IEVVSGGESVVRVAAQRPLTTDEVVAALAEAGDYTLVGA"
     gene            complement(19366..20061)
                     /locus_tag="PL014_03765"
     CDS             complement(19366..20061)
                     /locus_tag="PL014_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT56237.1"
                     /translation="MSSRSYAISHGVVAPLSKLLWRPIITGTENVPPHGGVLLASNHL
                     SFIDSFAIPIAAPRPVSFLAKSDYFTGTGVSGSIRRAFFEGTNAIPVNRHSSRAAQES
                     LDAALVVLREGRAFGIYPEGTRSRDGRLYKGRTGVAWLALTAGVPIVPVGLIGTDKVQ
                     PIGGHFPRLAKVHVSFGRPISPQAYAGMPSGKARRMLTDEVMDAIAALSGQERVDAYN
                     ELPPGSTGAAVGI"
     gene            complement(20058..>20837)
                     /locus_tag="PL014_03770"
     CDS             complement(20058..>20837)
                     /locus_tag="PL014_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HOT56238.1"
                     /translation="STSTPAQQAAPAQQAAPARPAGPGGIDTDAIRRSWPDVLEWLSR
                     HKRVTWTFVAQNAQVLDYDGQRVLLGISTVGLVETFRRGAHADYVRQALIDVLGVDAR
                     IDGQASDAVKPSQGEPVEPAPARADGSAGGPPQPESRPAPVPTPAAPADDAPRSPASG
                     PPPSAPAPASTPRAPVPPTREPASWSDAPPPVESAPSWASPFERAKAVVAEEVEQPAV
                     EHVVDDTAISDDDESIDDLADVGVPVVERILGGTVISEEPR"
BASE COUNT         3138 a         7371 c         7358 g         2970 t
ORIGIN      
        1 gcgaagtccg cgagctcctc gagcgagatc acggtgggcg gcagcatccg caccttccca
       61 gcgcggtgtc ggaccagcag gtcaccgggc cgaacccagg cgctgtggtc ggactcggtc
      121 gtggtgcccc gcgcctcctg gtcggcgggc atgccggcag cgaggatcca cgtgtcgtac
      181 cggcgcggct cgaactcggg agtcacccag tgggcccgac cccgcaggtc ggccacggtg
      241 atcacgaccc cacactcctc ctggacctcg cgcaccgcgg cagcagcgaa cggggccgcg
      301 acgtcggccg cgacacccat gaccgcggcc aggtcggcca gtccgggcgt cgccgggtcc
      361 aacgcgtggt ccaccgggtc gaccccgccg ccggggaaga cgaccgtcga gggggcgaat
      421 gccatcgacg ccacccggcg ctgcacgtag acctgcgggc cagccgcacc gtcgcgcacg
      481 agcatcacgg tggccgccaa ccgcggaacc gcaaccgagc gctccgcagc cgccagccac
      541 gcctgcgccc gcgcagccag cccggggtcg cgtgacaccg ggtgctcgac cacccgaccg
      601 gacgtcaccg acccaccccc agaaccaggg tcagtcgcgg acgtggacga tgacctcgac
      661 ctcgacgggc gccccgagcg gcagggcgac gacgccgacg gccgagcggg cgtgccgacc
      721 ggcatccccg aagacgtcgc cgaacagctc gctggcaccg ttgatgacgc cgggctgccc
      781 ggtgaacgac gggtcggacg ccacgaagcc gacgaccttg accactcgct cgacccggtc
      841 gaggtctccc acgaccgact tgaccgccgc aagcgcgttc agcgcacaca cggccgcgag
      901 ctccttggcg cgctccggcg acacgaaccc ctcaccctcg ccgacggcgc cggtcgcggc
      961 catgacgccg ccgaccatgg gaacctgccc cgagacgtac acccgcgagc cgtcgagcac
     1021 ggcgggtacg taggccgcca ggggcggcac gatctcgggc agcacaaggc cgagctcggc
     1081 cagacggctc tcgacggcac ccatgtcagg ccttcgggcg cttgaggtag gccacgaggt
     1141 tgccctcggg catgacgagc acctggacga ggtcccagcc gtcggcgccc cactggtcga
     1201 ggatctgctt ggtggcgtgc acgatgagcg gcacggtcgc gtactcgtag gtcgtcatgt
     1261 ccgcaaccct agcgccgggc cacccctcgg ctagcgtgac gcagatgagc gagcgcccag
     1321 ctgaccgccc cgccgaccgc accgagatca ccgggcccca ccagagcgac gtctacgacg
     1381 tctgggagcc cgccccccac cgggcccgcg gggtgagcgt cgcgctgatc cacggggggt
     1441 tctggcgcga gcgctacgac cggcaccacc tcgctcccct ggcccgggcc ctcgccgacg
     1501 acgggttcca cgtggcgaac ctcgagtacg cccgagcggg gatgccgggg ggcggctggc
     1561 cggggaccgg cacctcggtc ctcgcgcagc tcaccgcggc gcacgccgac ccggcgctgc
     1621 ccgagcgagt cgtcgccgtg gggcactccg cgggcgggca ccttgccctg tgggtggcct
     1681 cggcagaccg cgcgccgtgg ctgaccgggg ccgtggccct ggccccggcc gccgatctcg
     1741 gcgaggtcga ccggctcggc ctgagcgacc acgctgcccg caacctcatc ggcgccgccc
     1801 ccgatgacgc ccccgacacc tgggccgacg ccgacccggc gcgtcagcgc ctcaccacac
     1861 ccgcggtgat cgtgagcggc gagcacgacg acgacgtccc ggcatccgtc atcgagtcct
     1921 acctggccac gcgcacaccg gacgatccga tccacagcgc cgtcgccctc ggcgcagacc
     1981 acttcgccgt catcgacccg caggcgccgg cgtacctgct ggtcctcgcc gagatcgagg
     2041 agctcaccct ggccacccac tgacggccct gaccaaggat tcgaggatgc cgtgagggcc
     2101 cgcctaggct ggtgggcgtg tccactccct ctctcgcacg tgccttcgac ggggtgcgcc
     2161 tgcacatcgt gaccggcaag ggaggcgtcg gcaagaccac cgtcgccgcc agcctggccc
     2221 tgtcgctggc cagccagggc aagcgggtgc tgctcgtcga ggtcgagggg cgtcagggca
     2281 tcagccagac cttcgacgtg cccccgctcg gcaatgacga ggtgcgtctg gtcacccatg
     2341 ccggtggcgg tgagctgtgg gggttgtcgg tcgacgccaa ggccgcgctg atggagtacc
     2401 tacagatgtt ctacaagctc ggccgcgccg gcggagccct cgagaagatg ggcgtcgtcg
     2461 acttcgcgac gacgatcgcc cccggggtgc gcgacgtcct gctcacgggc aaggtctacg
     2521 aggcggtcgg gcgcacgacg ggcaaccgcc gcggccaggg cacgaaggtg tgggatgccg
     2581 tcgtcctcga cgccccgccc accggccgca tcacccgctt cctggacgtc accgcgcagg
     2641 tggccgacct cgcccggatc ggcccgatcc actcgcaggc cgagtcgatc acccgcatgc
     2701 tgcgccaccg cgagagcgtc gtgcacatcg tgaccctgct cgaggagatg ccggtgcagg
     2761 agagcgtgga tgccgtgact gaactgtccg cggcaggctt cggggtgggg acggtcatcg
     2821 tgaacctcgt gcgcgagccc atcgtcgacg aggccctgct cgaggtcgcc gccgccgagc
     2881 cggcccgggt cgccgaccag gtgcgggccg acctcgtctc cgtcggggtc aagacgtcga
     2941 aggccaccgt cacggggctg ctcagccagg cgcacgacca cgccgagcgg gtggcgctcg
     3001 agcgcgacct ggctgccgag gtcgcggccc agggcctgcc gacgctccgc ctgccggcgc
     3061 tgccgaccgg ggtcgaggac ggcggcatca ccgtgctcgc cgacgccctg accgcgcagg
     3121 gggtgcgctg atggcccgcc gccccgcacc cgcccggctc gacgtcgacg cactgctggc
     3181 cgaccccgac acggggatca tcgtgtgttg cggctcggga ggggtcggca agacgaccac
     3241 cgccgcggcg ctgggggtgc gcgcggccga gtccgggcgc cgcgtcgtcg tgctgaccat
     3301 cgacccggct cgacggctcg cccagtccct cggcctgacc gagctcgaca acgtcccacg
     3361 tccggtgaag ggcgtcggcg aggaggccgg cggcagcctc gacgcgatga tgctcgacat
     3421 gaagcgcacc ttcgacgagg tcgtcgaggc gcactccacc cccgacaagg ccgcgcagat
     3481 cctggccaac cccttctacc aggccgtctc cagctcgttc gcgggcaccc aggagtacat
     3541 ggcgatggag aagctcagcc agctgcgagc ccaggccgac cgcgagggca cctgggacct
     3601 cgtgatcgtc gacaccccgc cgtcgcgctc ggccctggac ttcctcgacg cccccaagcg
     3661 gctcggctcc ttcctcgacg gccggttcat ccggctgctc accgcgccgg cccgtgctgg
     3721 cgggcgggcc tacctcaagg tcttcagcgt cagcgcgacc atcgccgcgg cgacgatctc
     3781 caaggtgctc ggaggagcgt tcctgtccga cgtgcagacc ttcgtcgcgg ccctcgacac
     3841 gatgttcggg gggttccgcg agcgtgcgga cctcacgtac gcgctgctca aggagcccac
     3901 gacggcgttc gtcgtcgtcg ccgctcccga acgtgacgcc ctgcgggagg ccgcctactt
     3961 cgtcgagcgg ctcgagagcg aggggatgcc gctcgcgggc gtcgtcgtca accgcatgca
     4021 ggcgctggcc gcaccgtcac tgagcggggg tcgggcgacc gcggctgccg agcagctcga
     4081 ggatgccggg ggcagcgagc tcactccggc gctgctgcgc ctgcacgccg acctgtcctc
     4141 gatcgccgag cgtcacgagg ggcacgtgcg ccggttcgtc gccggccacc ccggggtgcc
     4201 ggtctcgacc gttccggcga gcgcgaccga catccacgac ctcgacgggt tgcgcaccgt
     4261 gggcgcagcg ctggcagcca cctgaggcgc aggccacctt cccgtagggc ggggtcaggc
     4321 gctctcgccg tggaccgctg ctcccttgac ctgggccagc aggccgctcc acgactggac
     4381 ctcaggtcgg cggcgcagca gggcccggcg ctcgcgctcg gtcatcccgc cccagacacc
     4441 gaactccgtg cggttgtcga gagcgtctgc gaggcactcc gccatcaccg ggcagccctt
     4501 gcacatgacc ttcgcggcgc gctgagcctt tccctggacg aacaaggcgt cagggtcctc
     4561 gtccgcgcag tggcccaggc cgacccagtc ggcaacccag tcccccagcg ctggtgctac
     4621 ctcgggtgcg gcgctcatgt gtacccctgt tcagcaagaa cctggaacgg ctcgaacagc
     4681 ccggtcgtgc gtaccgaccg cgcgttgtca ggctagggcg gtcgatgaca ccacggcatc
     4741 gtccgaatcg tcgcgagcaa tggccgaacg gttgattcct gaggcatcgg tgttcacgct
     4801 caagcttcat cggtcgacaa acgtcgacgc cgccagacca gcgcgacacg cgcgagcgag
     4861 ggccgacggc atccacgggt taccctgatg gtatggacgg tcgcgcccac aatgtatccg
     4921 ctgtgctcag attgcttctc ggtttcgtcg tgctcagcac gattgccggg ttgttgatgg
     4981 ctgggctggc gatccctgcg gtcggggcca ccggtcagat ggccaagggg ggcgtcgggg
     5041 tcttcaacga cctgccgagc gagttcacgg tctcaccgct ggcgcagcag tcgcgcatcc
     5101 tcaacgccga cggcaaggtg atcgccaacc cctacgacga gaaccgcatc atcgtgccgc
     5161 tggcgaagat cgcgaagaac atgcagaacg cgcagctggc catcgaggac tcgcgcttct
     5221 acgagcacaa cggcctcgac gtgcgcggct tcagccgggc catggtctcc aacctccagg
     5281 gcggcgacac ccagggagcg tcgaccctca cgcagcagta cgtcaagatc tcgctgcagt
     5341 accgcgcgct gagcaacggg gacaaggatg ccgccgccgc tgcggtcgag aagtcctact
     5401 cccgcaagct ccaggaactc aagtacgccc tgaacgtcga ggagaacttc accaaggagc
     5461 agattctcga gggctacctc aacctcgtgt actacggcga ccaggcctac ggcgtcgagg
     5521 ccgcggccca gaactacttc ggcgtctccg ccgccaagct caacctcggc caggcggccc
     5581 tgctcgccgg catcgtccag cagcccacgg cgttcaaccc ggtgctgcac cccaaggccg
     5641 ctcagtcccg ccggaaccag gtcctcgacc gcatgcaggc actcgggctg gcgtcggcca
     5701 aggacgtcgc cgctgcgaag aagcaggagg tcgcaaaggt cgtcaagcgc aagccggcca
     5761 agggtgtctg cttccggtcc cccgagccct acttctgcgc ctacgtcatg gagtggttgc
     5821 agaagtcgcc ccagatggcc gtgctgggca agacccccgc cgagcgcctg aagaccatca
     5881 accagggcgg gctggtcatt cgcaccaccc tgaacccggc gatgcaggcg agcgccaaga
     5941 aggaactcgc caaggccgtc aagccgaaca acaagcagaa cctcgccggc gccgtgacca
     6001 tgatcgagcc gggcacgggc aaggtgctcg cgatggttca ggccacggac ttcgcgaagt
     6061 accagaccaa catcaacgtc gaccaggagt acggcggcgg ccccaatggc taccagatcg
     6121 gctccacggc caagaccttc gcactcgtgg aggctctcga gcgggggatg ccgctcaacg
     6181 cgaccatcaa tgtccccgcg tccagcccga ccaagcccgc ggtgttcgag ccgaagaaca
     6241 tggttgacga gtgctcgacc aacgagccgt ggccggtcaa gaacgacttc tcgatgagcg
     6301 cagggccaat gacgctgcgg gaggggacgg tcaagtcggt caacccgttc ttcgcggctc
     6361 tcaacgtgcg cctgggcgcc tgctcggtgc gcgacacgat ggtgaagatg gggctgcacc
     6421 gcgctgacgg caagcccatc tcggacacca tctcgggcat cgctctcggc gccggtgagt
     6481 cgaccccgat gagcgtggcc agtgcctacg cgaccctggc cgcgaacggc aagtactgcg
     6541 agccccagcc cgtcaccgcc atcagcaccc ccgatcgcaa gagcctgaag atccccgcag
     6601 ccaagtgttc gcaggtcatc tccgccgatg tcgccgctgg cgtgactgac ctgctgcaga
     6661 gcgtggtcaa ggtcaacctc gggtcaatgt ggagcagcag cgcccggccg gcagccggga
     6721 agaccggaac gaccgagcgg ttcaaccagg tctggttcgc cgggtacacc ccgcagatct
     6781 cgacggtggt gttggtcggc aacctcaaac cagcgaacaa gaacggcaag ctctacaccc
     6841 tgcgcggcaa gtgcttcggc gactacgggt gccccagccg ggtgtacggc tcaaccgtgt
     6901 ccgcacccat ctggtccaag atcatgaaga ccgcccacaa gggaatgccg gtcaaggact
     6961 tcaagagtcc gtcggcggac atccgcaagg gcaactacgt gcgcctgccg aacgtcatcg
     7021 gccgcggccc cgacagcgcg ctcgcacgtc tgaaggaggc cggcttcagc ggcttcgtgg
     7081 ctggccgcac caacagcagc ctgcccgccg ggactgtcgc cttcaccgac ccctcgggct
     7141 cggccctgcc gggtgggcgg gtcggcctgt ggctgtccaa cggctacaca ccgccaccga
     7201 ccgcccccaa gaaggcggct gccccggaga aaccccagca gactccggag ccggccaaga
     7261 agcccggcaa gccagcgccg aaaccgacga agagctagcg gatgagatgg ggcccgagcc
     7321 gcctactggc ggctcgggcc ccatctcatg tcccggccct gtcggcctat gccccggaca
     7381 gcacctgctt caccacggca gccacccggc cgccgtctgc ccggccagcg acctcgccct
     7441 gggctgcctt catcaccatg ccgagctgcg gcatcccggt ggcgccggtg gcggcgaccg
     7501 cccggtcgac gatctcgcga agggcctcgt cgtcgagctg ggcgggcagg tagccctcga
     7561 gcacgagcag ctcggcctcc tcggtggctg cgagctcagg tcggccggcg ccggtgaagg
     7621 cggcggcggc ctccttgcgc ttcttggcct ccttggcgac caccttgagg acctcgtcgt
     7681 cgctgagctc gcgcgcctcg tcaccagcca cctcggcgtt ggtgatggcg gtcaacgtca
     7741 tccgcagcgt gcccgagcgc acggtgtcgc gtgcgcgcat ggcgtcgtgc aggtcgtgct
     7801 ggaggcgagc cttgagcgtg tcgtcggtca tggccccagt ctcccaccgg cgattgggtg
     7861 gggcgccacc ggtttgcgag gatggagaga tggacgacct ggctcgcacc gcgctgcgca
     7921 ccgctggtgg cctcgccggc gccggtctcg tcgggatcgc gtacgccgcg ttcgtggaac
     7981 gcacctggtt caccctgcgc cgcttcgccg ttcccgcact gccacccggg gcggctccgg
     8041 tgcgcatcct ccaggtctcc gacctgcacc tgacgcccgg ccagaccaag aagatcgact
     8101 gggttcgctc cctggccgag ctcgagcccg acttcgtcgt gaactcggga gacaacctcg
     8161 cccacctgga tgccgtgcca ccgctcctgc gagcgatgga gccgcttctc gagcggccgg
     8221 gtgcgttcgt cctgggctcg aacgactact tcgccccgac gccgaagaac ccggcccgct
     8281 acctcaccag ccgccacgcc gacgccccgg cccgacgctc gacgctgccc gtcaaggcgc
     8341 tgcgcgacgg cttgggcagc aagggctggg cagacctgaa caacgctcgc aggatcgtgc
     8401 ggatggggga ccacgatgtc gagctcgtgg gggtgaacga cccacacatc ggctacgacc
     8461 ggtacgcgaa ggtggccggt ccggcatcca ccgacgtggc cctgacgatg ggcctggtgc
     8521 acgctcccta ccagcgggtg ctcgacgcca tggtcggcga cggggcatcc gtcgtgctcg
     8581 ctggccacac ccacggtggg cagctggccg ttccgctctg gggtgccctg gtgacgaact
     8641 gtgacctcga caccaggcgc gccaagggcg tctcgaggtg gtggccgggc gccggcaccg
     8701 ccggtgctcg agggggcgcg gcaccctcct cggatgctcc cgaggatgcc gcgtggctgc
     8761 acgtctcagc ggggctggga acctccccct acgctcccgt gcgcttcgcc tgccggcccg
     8821 aggcaaccct gctgaccctg ctggcccgcg acagctgagg ccgatttctg tacgccgcac
     8881 cgggtcggct atcctcgggt gtcgccgcac ccccgggtac ggctaacagc cacggggtgt
     8941 ggcgcagctt ggtagcgcgc gtcgttcggg acgacgaggc cgcaggttca aatcctgtca
     9001 ccccgaccag atgatggcgg ttgctcgaca gtctcgggtg gccgccatca cgcgtctcgg
     9061 gcccggcccc cggaacgcct tccacgagct cctgcaggca ggtgtgcgga cgcactgctc
     9121 caacccgtgt acgtcacgaa cacgaacgtc agatgccgct cagacccttg ctcagcagga
     9181 gcgcaccgat gacgaggacg acgacggcca tgatcgtcgc gttgtgcgtc gtgagccagg
     9241 cctcggcccg ccgcaacggg gcctgcgcgc ggtccccgag cacgaggtgc acgccgatcg
     9301 gcagggccac cgacagcgac ccgacgagcg cgaaggcgat gacggtcgcc actgttgccg
     9361 cggtgccctg ctcctcggca gcgatggtca gaccggccgc tgcgctgagc agcaggatct
     9421 tcggattggc tcccgagagc agcgcaccga gcctcagcgc cgacggcggg gtggactcac
     9481 cgagcgcccg catccacccc gggggctctg cgttcgcgtg gcgggtcagg aaggcgcgca
     9541 cgcccacgac gacgagcacc ccgccgagca gcacgcgcgc ccacgacacc caggttggcg
     9601 tctcctcgaa cccgtcgatg acggtcgcca ggcccacgaa gagcgcggtg ccggcggcga
     9661 cccctgccgc ccagccaccg aggaacgaca cggcgttggc ggtcgcccgc ggggtcagca
     9721 ggagcaggat caccgggatg acggggaagg gcgaggcgag gatgccgatc accagcggaa
     9781 cgaggtcggc caccatcagc gtcggcttcg tacgaccggc acgagcgccg cggacccgaa
     9841 gagatccagg ccgagggttc gcgagagccg ctcggcggcg aggatgcccc gaacactgtt
     9901 gccagcggca tccagtggtg gggagaacgt cccgaggcca cccttgccgg gcgcggccgt
     9961 gacgatcccg ccgccgatgc cgctcttgcc gggtagcccg acccgcacca gccactcacc
    10021 tgaggtctcg tacatcccgg ccgtcgtcat caccgccagc gtgcgcgccg cgacctgagg
    10081 gctcaccacg gcgacgcccg tgacggggtt gactcccccg tcggcgagcg tggcccccat
    10141 gacggcgagg tctcgggcgg tgacggccag cgaactctgc ctggtgtaca ggtcgacggc
    10201 gccatccggg tcgtcgaggc ggccgagaga gtgcagcaac tgccccaggg cccggttgcg
    10261 ctggttggtc gcgcgggcgc tgtcgtggat gccgtcgtcg agcgacaggt cgcggcccgc
    10321 gaaggcggag agtccggcgc gcagctcgtc ccacgcggca tcgaccgtga agtcgcggtt
    10381 tcgccccagg aacaggccgg tgcaggcgat ggcacccgag ttgaccatcg ggttggtgcg
    10441 cccgtcggga ctgtgctcga cggcggccag cgagttgaac ggtcgcccgg tggcgtcgac
    10501 gccgatgtgg tcgacgaccc catcgacccc gaggcgagca caggcgaggg cgaagacgaa
    10561 cggcttggcc acgctcatga gcgcgaacgg gacgtcgacg tcgccggcga cgaactcgtg
    10621 cccgtcgacg ccgatgaccg cgatgccgaa caacgacgga tccgcctgtg ccagcgcggg
    10681 atacaccgtg ctgacacgac ccgcgtcgac agttgccacc tcggcgtgac aggccgccac
    10741 cgcctcacgg acccactcgt cacgggggag gtgcccggtc gagacgtagg tcgcacccag
    10801 atcagcggtg gcgccgcgcc ccggcaggga cgagccggga tcggtcatgt cgcgatggta
    10861 tgccgcccgg cgcctcagat ggcgtcgtcg cgaaggatcg tgccgtcggg gtcgaacgcg
    10921 tcgtagagcc ggggcgccca cagcatctgg gccctgctca gctccggcgg ggagagccgg
    10981 cgcagacggc ccggtggtcg ttcgacgccg tcagcgccgg accacgcccc cgaacgccga
    11041 cgccaccgca ccgatgtgcc cttcatcggc cccttgggcc ggggcaggtg ccgacgccca
    11101 cgggggcccg cgccggatgc gtaccactcg tcaagggcat cagcccgctg cgccatgagg
    11161 tcccacagct catcggcgtc atccggcacg tcctcggggt ccagaccggt gtgctcaccg
    11221 acgagctcgc gccgcagccg ccgggcgaag ctgtcacgcg gtgccggctc gtcgagcccc
    11281 cgcacccgcg tgtcctgcac ggcacaggcg atctcggagt cgctcgacca cgacctgcgg
    11341 ttgaagttgt cggagccgac gctggaccac acgtcgtcga tgacacacac cttggcgtgc
    11401 acgtagatcg ggtagccggc gtcgctcgtc agcccgtaca ccgcgacgcg gtcacccccg
    11461 gcctcgagca ggaggtccat ggcgagcttg cgcccgtaca gctgcggcgg gagggacacg
    11521 gcaccgtcga cgtccggcac catggggatc accgcgatca gccgcaactg cgggttggcg
    11581 cgcagggcct caccgaagtg ccggccgacc tcctgcgacc acaggtactg gtcctcgagg
    11641 tagatcaggt gtcgtgcgtg cgccaccgcc ttggtgttgg catgggcaat gctgcgctcg
    11701 ccacgggggg cgaagtcgta gcccttgggc aggatcgacg ggtaggtgcg cacgatctga
    11761 accgcctcgg atgccgtcgc gcccgcctcg gatgccgtcg cccccccacc ggatgtcatc
    11821 tcgcccacct tgggtggtgg cgggccctgc ggggacaggg gttggggctg gaggtcctca
    11881 ccctgcagca ggctggacgc gagccgcccg gggttgagcg tcagcggggt cgagtcctcc
    11941 cagcgctcac ggaacgtggt ctcgacgtcg gcgacggccg ggccctggat ggccacctgc
    12001 acgtcgtgcc acggcggccg cgcaccgaac acctgaggca tggcgatccg ctgggggtca
    12061 ccctcgtggg cgatggagtc acgccgaccg tggcagaggt cgatcccgcc gaggtaggcg
    12121 atgtcgcggc tgggctcgtc acggtgtcgc agcaggacga acttctggtg gtgcgagcct
    12181 ccggtgcgca cgcgcatgtc gcgcaggcac tgcccgccgg cctcgtcgat ctcgatgccg
    12241 agcaggcgtg acttctggga gtggaagccg aaccggtgcc agtgcgagcg ccacagcaac
    12301 cctctcacct cgaccccgcg ccgggcggcc gcggcgaacg tcgcggagac gccggacccg
    12361 gggtcatccg tgagcagctg gtcggggtcg ccgcgccagt ccacgaagta gagcaggtcg
    12421 ccgtcgccga gcgcttcgat gcgttcgtgc agctccgcca ggtagggggc cccgtgcacg
    12481 atcggccgca cgtggttgcc ctcggtccag gccacgcccg gaggctttcc ctcgtcgatg
    12541 cgggtgtcgg gattctcccg ctcgtcgggg gtgaggaaga gatcggggat cacggcctca
    12601 tcctgccccg cgaaccctcc ctcagatggc gaagtaggcg atgacgatgg cgaagatcaa
    12661 cggcacgccg aacacgacgt ccaacacgtg gaagggcacc cagaacaggt gggtgacctt
    12721 ccacccgtag tcggcctccg gccactcggg cggcacccag gaacgggcgc gccagagccg
    12781 ctgaccgacc aggagtacgg cgtagatgac gaccgcgacc caccaggcgt caaccccgac
    12841 gtactggctc acgatcagct gcctcgtcag gcctgaccga gcgattcctt cagctcacgg
    12901 aaccacggca cggtgacgtc gaacttcttg tggccctgga tccgggggaa ctcgatcgcc
    12961 cgcagcgtgt cgccctgctc ctcgtcgtgc ccgacgacac ccgacacccc ggccagcgcc
    13021 gagtcgaccg cgagcgaggt gcactgcgcg atgagggcga ggtcttcgtc gttggccgcg
    13081 gccgagcggc tgaagtagcc ggacttctgg accatggtct tctcggcctg gagcttcgcg
    13141 ccgaactgct tggcgaacca ggcaccgggg ttgaccttgt cgagctgcac gtgaccgaac
    13201 gggtcgaccg gcacctcctg gccggcggcc ttgagctcct cgacgacctc ggccacgccg
    13261 gcgccctcgg agaggaagat gttgacgcag ccgatctcgt ccatgatcgc cttgaggcgc
    13321 tcggcctcgg ccgcgatgtc gatgtgctcc tcgggcacga acacggcgtg cacgtcccag
    13381 cagcgcgggt cgttgccgaa gccctcgacg aactgctgct cggcgaccca tgcgtggtag
    13441 cggcgagcgg tctcggccgt gagccagcca cagttgcggc ccatgacctc gtggacgatg
    13501 agcatgcggg ggttcgagga gtgctcggcg atgatgttct gcgcgtagat cgagccctgc
    13561 tcggcggccg tccacgcccc cagcgactgg cggatcggca cgacgtcgtt gtcgatcgtc
    13621 ttgggcaacc cgacgacggt cagctcgtag tcgttctcgt gcaggtaggc cgcgaggtcg
    13681 gctgcggtgg tgttcgtgtc gtcgccaccg atggtgtgca gcacgtcgac accgtccttg
    13741 gtgagctgct cggccgccac gtgcagcggg tcctgaccct cctgcaccag accacgcttg
    13801 acgcagtcgg cgacgttggt cagcttgact cgggagttgc cgatcggcga accgccgcgc
    13861 aggtggagca ggtgggcgtt ctcgcggacc tccggcgtca cctcgaccca ctcgccgcgc
    13921 agcaacccgg catacccgcc gaggtaggcg atgatccgca cgtcgggagc gacctcgccg
    13981 tagcgctcga tgagtccgcc gacggcggag gacaggcacg gtgcaagccc tccggcggtg
    14041 agcatggcga cggtacgaac ggtcatgggg tgctcctgat gtccggtgct ggtggtctgg
    14101 ggtgctcgat ggtgcgcgac gacgacgccg gcgccacggc atccattgtg gtggtttcga
    14161 cgagatcgcg tgcgacgagc tcagcggtct ggaccatgtt cagggccgcc cccttgcgca
    14221 ggttgtcgcc gctgatgaac aggtcgaggg tcatcggcgc atccggtgcc tggcggatgc
    14281 gcccggcgaa gcgcgggtcg gccccgacga cgtcgttcgg tgtggggaac tcgggctcgt
    14341 cggggtcgtc gaggacgacg atggtcggtg cctcgacgag ggcttgccga gcttcgttga
    14401 cccggacctt gcgcgcgaac gtggcgtgga cgatgacgga gtgcgacgac accaccggca
    14461 cccggatgca cgtggtcgtg atgggcaggt cgggcatgcc gaggatcttg cgggtctcgt
    14521 cgcgcacctt gacctcctcg ctcgtccagc cgtcgccgac gtggtggccg acgaagggga
    14581 tgacgttgag cacgagcggc gcgggaaaga ccgactcgcc cagctcgtgc tcgacgaggc
    14641 ggcgcacgtc gccggggcgc gtcccgagct cacgctggcc gtggacgacg tcgagctcgt
    14701 cgtgcagccg gctgatccct gcccgaccca gaccggatgc tgcctggagg gtcgtgatga
    14761 ccagttcggt gagctcccag gcggcgtgga gcacggcgag ggcgtcgatc atcgtcatga
    14821 ccgtcgcgcc cgggttggcg atgatgccgc ggggccggtc ggcgacccgt tgcgggttga
    14881 cctcggggac gacgagcggc acgtcgtgct cggtgcggaa gacggtgctg ttgtcgatgg
    14941 ccacgacccc gcgggccgcg gcgagctcga cccactcgcg ggcaatcccc ggcgggatgt
    15001 cgaagatcgc gacgtcgacg ccgtcgaaga actgcggggt cagcgcctcg acgacggcat
    15061 ccttgccggc cacccggagc accgtgccga cgtcctcgct acctgccgcg aggcgcacct
    15121 gcgcccagtg ggagtgcctc gtgggcagca cgtcgaggac gacacgaccg accgcacccg
    15181 tggcaccgac caccgcaagg gtcggctgcc aaccctgggg ccgtgcctgg ttggtgccca
    15241 cgtcagcggc cggtgccccc gtagacgacg gcctcgccct ggctggagtc cagcccgaag
    15301 gccgtgtgca cagcgcgaac ggcgtcgtcg aggaggtcac cgcgggtgat gaccgagatg
    15361 cggatctctg aggtggagat catctcgatg ttgaccccgg cctcggcgag ggccttgaag
    15421 aacgttgcgg acaccccggg gttcgatcgc atgccggcac cgacgagcga gagcttgccg
    15481 atctggtcgt cgaactgcac gccgctgaac ccgacgtcgg cctgcacccg ctgcagggcc
    15541 tcgaccgcgg cggtgccgtc ggtcttgggc agcgtgaagg agatgtcggt gaggccggtg
    15601 gcggtggcgg agacgttctg gacgatcatg tcgatgttga tctgggccag cgtcaccgcc
    15661 tcgaagatct tggccgccat gccggtgcgg tcgggaacgc cgacgacggt caccttcgcc
    15721 tcgctgcggt cgtgggccac accggcgatg atcggggctt ccacggcgtc tccttcaggt
    15781 ccttcgggtg cggggtggtc ggtcacgatc gtgccctcct tgtgggagaa cgacgaccgc
    15841 acgtggatgg ggatgtcgaa gcggcgggcg tactcgacgc tgcgcaggtg aagcaccttg
    15901 gaccccgagg ctgcgagttc gagcatctcc tcgttggaga tgcgatcgag cttgcgggcg
    15961 ctgggcacga tgcgcgggtc agcggtgaag acaccgtcga cgtcggtgta gatctcgcac
    16021 acgtcggcgc gcagcgcggc ggccagcgcc acggcggtgg tgtcggtgcc gccccggccc
    16081 agggtggtga tctccttggt gccctggctg acgccctgga agccggcgac gatgacgatg
    16141 tgacccttgg ccagggcctc ggtgatgcgc cccggggtga cgtcgatgat cttggccttg
    16201 ccgtgcacgc cgtcggtgat gacgccggcc tggctgccgg tgaaggaacg ggcgctgtag
    16261 ccgaggtcgg agatcgccat cgcgaccagg gccatggaga tccgctcacc tgccgtcatc
    16321 agcatgtcga gctcgcgcgg cggcgggacg gggctgacct cctgcgcgag gtcgagcagg
    16381 tcgtcggtgc tgtcgcccat ggccgagacg gccacgacga cgtcgttgcc ggccttcctc
    16441 gtctcggcga tccgcctcgc gacccgcttg atgctctcgg cgtcggcgag cgacgatccg
    16501 ccgtacttct ggacgacgat gggcaacgtg ctactcccgt gacggtggtt cggtgggctc
    16561 tgacactcta accggccgtc cacggagcgg acatgatgcc cagatgccgg gcgaacagca
    16621 tccgggtgcc ccgaaccacc ccgtggggcg tcccgcggcg tagcgtcgac agggaccact
    16681 cgaggaggac cacatgtcga tcgtcatcat cggagccggc ctggcggggg gcaccgccgc
    16741 gaccgagctg cgcgagcagg ggtacgaggg caccgtggtc ctcgtcgggg ccgagacgca
    16801 cccgccgtac gagcgcccgc cgctgtccaa ggggtacctg ctcggcaacg actccctcga
    16861 ggacgcgttc gtgcaccctc gcgactggta cgccgagcac gaggtcgacc tgcggctcgg
    16921 ggtggaggcg accgggctcg acaccgacgc gcacgtcgtg tccacaaccg gcggcgacct
    16981 gacctacgaa cggctgctgc tggcgaccgg cgccgagccg cgccggctcg ccgtcgccga
    17041 ggacagcggc gtgcccaccg cgtacctgcg caccatcgag gacagcgacc ggctccggga
    17101 cgccttcgcc gccggcgggc ggatcgcggt cgtgggggcc ggctggatcg ggctcgaggt
    17161 ggccgcggcc gcgcgcgaga agggctgcga ggtgacggtg ttcgagaccg ccgagctacc
    17221 gctgctgcgc gtgctcgggc cgaccgtcgc ccaggtcttc gccgatctgc accgcgagca
    17281 cggcgtggac ctgcgcctgg gcacgtcggt cgcggcggac gacctggcgg gcgccgacct
    17341 cgtcgtcgcc ggcgtcggcg tgagcccgcg cctggacctg gcccgctcgg gtgggctggc
    17401 cgtggacgac ggcgtgctcg tggatgccgg gctgcgcacc tcggtcgcgt cggtgtacgc
    17461 ggtcggagac atcgcgagcc acgcgcaccc cgtgctcggg cgccggatcc gcgtcgagca
    17521 ctgggacacc gcgatcaacc aggccaaggt cgcggcgcac aacctcgccg gggcggacga
    17581 gacgtacgac gcgatgccct acttcttcac cgaccagtac gacctgggca tggagtacgt
    17641 cgggcacgcg agcggcgacg acgaggtcgt ggtgcacggt gacctgtccg cgcggcagtt
    17701 ccgggccttc tggacgcgcg agggccgggt cgtcgcggcg atgcacgcca acgactggga
    17761 cgcgaccgac gacctgcgcg ccgcggtcga gtccggcgag ctcccggcat ccccctgacc
    17821 ccggcgacgg ccgggcgggg gtcaggggtg gagggcgtcg aactgcgcct cggacacggc
    17881 atccggatcg gcgtccaggc gcacgtgcgc gagcagggtc tggaccactc gtagggcggc
    17941 cgcggcccgc tctccccagt gcgagaggta gctgaactgc caccaccaca gggcctcgag
    18001 cgtgcggccc tcctcgtagt ggctgaggcc gtggctgagc gctgaggcga tgtcgacgag
    18061 gtcgttgctg aggtaaccga cgacgggctc ggggtcgagc accgggtcga cgacgtcgcc
    18121 gtactcgtcg aggccctcga acacgttggc caggccggcg cgcaacgggt cgaactcggc
    18181 atccggcccg gcatcgggtt cgaagcgctc ggccgggacg acgtcctcca cggcacccag
    18241 cctggccccc atgacgagca cctgcgacag cgcgagcagc gccatcggca aggtggcgtc
    18301 cggggagttt ccggcagcga tctcgcgcac ggtcacgagg tacatctgcg cgtcggcggc
    18361 cgactccgca gcgatctggc cccagtcgtc ggaggcggag gcggaggcga ccgagaactc
    18421 agacatcgat gagcctgcgt ccttcgaagg cacgcccgag ggtgacctcg tcggcatact
    18481 cgaggtcacc gccgacgggc aggccggagg ccagtcgcgt cacccgcaac ccgaagtcct
    18541 tgagcatgcg cgacaggtag ctggccgtcg cctcaccctc gaggttgggg tcggtcgcga
    18601 tgatgacctc ggtgaccgtg ccgtcgccga gcctcgtcat cagctcacga acccgcaagt
    18661 cgcccgggcc gatgccgtcg atggggctga tcgcgccacc gagcacgtgg tagcggccgc
    18721 ggaactcacg ggtgcgctcg accgcgacga cgtccttggg ctcctcgacc acgcagatcg
    18781 agtgcacgtc acggcgcgga tccagacaga tccggcactg ctgcgcctcg gcgatgttga
    18841 agcacgtctg gcagaagcgc accttggcct tgacctcggt cagggcgtgg gccagccgct
    18901 cgacgtcggc cgcgtcggac tgcaagaggt ggaacgcgat tcgctgcgcg ctcttggggc
    18961 cgacacccgg cagcctgccg agttcgtcga tgaggtcctg gaccgcgcct tcgtacacgt
    19021 gatgagccta cgacctcgcg gcaaccctgt ctcccgacgc cgcgcagcag atccgggtga
    19081 ttcggtcagg cgccgacgag ggtgtagtcg cctgcctcgg ccagagccgc cacgacctca
    19141 tcggtggtca gtgggcgctg ggccgcgacc cgcacgacgg actccccgcc gctcacgacc
    19201 tcgatctcga cgccgtcgac gccgtcgagg gcgctcagct cgtcggtgac cgcgtgggcg
    19261 cagtggccac aggtcaggcc cgtggcccgg aacgtctgcg tgctggcagc ggtgttgctg
    19321 gtgtcatcgg tggtcacggt gtctccttct cagatggggt gtggttcaga tgccgaccgc
    19381 ggcaccggta gagcccggcg gcagctcgtt gtaggcgtcg acccgctcct ggccggacag
    19441 cgctgcgatg gcgtccatga cctcgtcggt gagcatccga cgggccttgc ccgacggcat
    19501 tcccgcatag gcctgcgggc tgatcgggcg cccgaacgac acgtgcacct tggccaaccg
    19561 ggggaagtgc ccgccgatcg gttgcacctt gtcggtaccg atgaggccga ccggcacgat
    19621 gggcaccccc gcggtcagtg ccagccacgc gacccctgtg cgccccttgt agagccggcc
    19681 gtcgcgcgag cgggtgcctt cggggtagat gccgaacgcg cggccttcgc gcagcacgac
    19741 cagcgctgcg tcgagggact cctgggcggc gcgcgaggag tgccggttga cgggaatcgc
    19801 gttggtgccc tcgaagaacg cccgcctgat cgacccgctg accccggtgc cggtgaagta
    19861 gtcggacttc gcgaggaagc tcaccggccg cggcgccgcg atcgggatgg cgaaggagtc
    19921 gatgaaggac aggtggttgc tcgcgagcaa cacccctcca tggggcggga cgttctcggt
    19981 gccggtgatg atcgggcgcc agagcagctt gctgagcggc gcgacgacgc cgtggctgat
    20041 ggcgtacgag cgcgagctca tcggggctcc tcggagatga ccgtgccacc gaggatgcgc
    20101 tcgacgacgg gcaccccgac gtctgcgagg tcgtcgatcg actcgtcgtc gtcgctgatc
    20161 gcggtgtcgt cgacgacatg ctcgacggcg ggttgctcca cctcttcggc gaccactgcc
    20221 ttggcccgct cgaacgggct ggcccacgac ggcgccgact ccaccggcgg cggcgcgtcc
    20281 gaccagctgg ccggctctcg ggtgggcggc acgggtgcgc ggggggtgct ggctggcgct
    20341 ggcgcgctgg gcggcgggcc ggatgccggg gagcggggcg cgtcgtcggc cggcgctgcg
    20401 ggtgtgggca ccggcgcagg tcgggactcc ggctgaggtg gaccgccggc gctgccgtca
    20461 gcacgtgccg gggcaggttc caccggctcg ccctgggacg gcttgaccgc gtcggaggcc
    20521 tgaccgtcga tccgcgcatc gacgccgagg acgtcgatga gggcctgacg cacgtagtcg
    20581 gcgtgggcgc cgcggcggaa ggtctcgacg aggccgaccg tggagatgcc gagcagcacc
    20641 cgttggccgt cgtagtcgag gacctgcgcg ttctgcgcca cgaacgtcca ggtcacccgc
    20701 ttgtggcggc tgagccactc gaggacgtcg ggccaggacc gccggatcgc gtcggtgtcg
    20761 atgccgcctg ggccagccgg tcgcgcgggg gctgcctgct gcgcgggggc tgcctgctgc
    20821 gcgggagtcg aggtgct
//
LOCUS       DAOSOC010000026        19928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_2710_length_19928_cov_10.623862, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000026 DAOSOC010000000
VERSION     DAOSOC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 19928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19928
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_19928_cov_10.623862"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PL014_03775"
     CDS             complement(<1..1058)
                     /locus_tag="PL014_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT56239.1"
                     /translation="MPVSRRADLRNVAIVAHVDHGKTTLVDKMLWQSGAFGEHDHIAD
                     RAMDSGDLEREKGITILAKNTAVHYAGKAALDAGLTDGMTINIIDTPGHADFGGEVER
                     GLSMVDGVVLLVDASEGPLPQTRFVLRKALALKMPVVLCINKVDRPDARISEVVDEVY
                     ELFMDLMVDAEHHQDQLEFPIVYASAKAGRASMNRPADGGLPDGEDLEPLFRTIIETI
                     PAPSYDSDAPLQAHVTNLDASNFLGRLALLRVHNGTIRKGQQVAWCRRDGSVEKVKIT
                     ELLMTDALERKPAESAGPGDIIAVAGIPEITIGETLADVDDPRPLPLITVDEPAISMT
                     IGINTSPMAGRVKGAKVTA"
     gene            1311..2987
                     /locus_tag="PL014_03780"
     CDS             1311..2987
                     /locus_tag="PL014_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT56240.1"
                     /translation="MKLKRKAPVVLLTAAALTVTGCAQSDRESESTDTGTASGEFKDT
                     FTFGAAGAPKLFDPFYATDGETFRVTRQMHQGLLGVKPGTADVQPELAESWTPSADGL
                     TWEFKLKQGVKFSDGEPFNAEAVCANFTRMYESKGAAQTAAEYWGYFFGSFSDKPEGS
                     LYKSCEAKDESTAVIGITRATSSFPTILSLDSFSMQSPKAMAAGDAGNVQAAGEGFTY
                     PPYVMAPVGIGPYKLDKYDEANKTVTLVANDMFYGEKPKTAKLVFKIIPDESTRRQEL
                     QAGSIDGYDLPNPVDWKGLEDEGNSVEVRPAFNILYMGLNAKKNPVLKDLKVRQALSH
                     AINRDQMVKTQLPEGAQAASQFMPDTVSGYNTSLQPDAYDPEKAKALLKEAGAEGMTL
                     QFAFPTEVSRPYMPDPQKIHDAFKKDLEAVGIKVDVVSKPWNGGYLDDASAGNYDAWL
                     LGWTGDYNAADNFLGTFFSNYEQNDFQTSAYPWGKKLSEDLKAADAIVDEAARKAAYE
                     QLNKQIAQEYIPGLPISHSPPALVVSSKVQGLVASPLTAEMFDQVTVSSK"
     gene            3093..4103
                     /locus_tag="PL014_03785"
     CDS             3093..4103
                     /locus_tag="PL014_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56241.1"
                     /translation="MARFIIRRLIQMVGVVFVLSLLLFLWLRSLPGGPVSAILGERAT
                     DESRAALEKAFGLDQPVYVQYLKFLQRASQGDFGASTAVLPGRDALEIFFTRLPATIE
                     LSIIAMAIAIVLAIPLGYVAAKRHGSSIDNGAVVLSLIGVAVPVFFLAFLLKYWFAIK
                     TQTFPVSGRQDAALGCTRVTNFFVLDGLLTREFDCSADALRHLVLPAIALATIPFAVI
                     FRITRASVLDVLDEDYVRTAEAKGLTAQVIRARHVMRNALLPVVTTIGLQTGGLLAGA
                     VLTETVFNFGGIGSTLAQAFTQRDYAVLQVLILATATIYVLVNLLVDISYAIIDPRVR
                     AR"
     gene            4100..5071
                     /locus_tag="PL014_03790"
     CDS             4100..5071
                     /locus_tag="PL014_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56242.1"
                     /translation="MSPRNPMNARKQRIDDLAASSVIVDDAGAVDEAPGVSLIASAWR
                     RLRRDPVFLLGATITGLFIIVAIIAPWIAPHDPDARLLIDQVSRQSNPIPGPQDGFPL
                     GADDRGRDLLSRLIVGSRQTLLVGVAATLLGLFGGLVLGILAGAFGGAVDAFVMRAVD
                     VMLSIPSLLLAVSIAALARQPSQWTVIIAIAVVQIPVFARLLRGSMLAQRSSDHVLAA
                     RALGVKRSAIIFRHMVPNSLGPVIVQSTLVLATSIIDAAALSFLGLGNPDDAKPEWGQ
                     MLGQAQVYIQDYPHLAVYPALCIVFVALGFTLMGESLREALDPKSRR"
     gene            5076..6086
                     /locus_tag="PL014_03795"
     CDS             5076..6086
                     /locus_tag="PL014_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56243.1"
                     /translation="MTATASPPPTTTRGDAPLLSVRDLRVTFTRRGEKPFMAVDGVSF
                     DVRPGQTVGLVGESGCGKSVTSLAIMGLLPKRGNTVEGEASFEGVNLLTLDEKAKRER
                     RGRDIAMIFQDPLSSLNPVVPVGLQVTEVLERHRGMSRKAATPVARDLLKKVGIPDPD
                     RRLKDYPHQMSGGMRQRALIAMAVACAPRLLIADEPTTALDVTIQAQILALLKELVQD
                     TGTALVMITHDLGVVAGLCDEVNVLYAGRIVERGERHELFADPRHPYTNGLLASIPRL
                     DAPRGERLTPIPGSVADNLPWDSACAFAPRCSQPVDACVHTTPALDETGRRSLRCHNP
                     VR"
     gene            6091..7257
                     /locus_tag="PL014_03800"
     CDS             6091..7257
                     /locus_tag="PL014_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56244.1"
                     /translation="MTDTTTQATPPSGDERDVLVDVRGVKVHFPIKRGVIFDKVVGHV
                     YAVDGVDLQIRRGETYGLVGESGCGKSTLGKAILNLEPPTEGSVILDGTDIASLRGEE
                     LRRRRKDIQMVFQDPMSSLDPRQSVESLLVEGMRAHGLDTDGASTKKRLRELLAAVGL
                     PAAALTKYPHEFSGGQRQRIGIARALSVEPKLIVADEPVSALDVSVQAQVINLLEELQ
                     DEFDLTYLVVAHDLAVVRHISDRIGVMYLGGLVEEAGADDLYGQPLHPYTRALLSAVP
                     VPDPTIEDTREQILLTGDLPSPSNPPPGCRFHTRCPWRQETRCDDERPPLRVVQIDGV
                     DPSHRVACHWVEQIASGEIRPHEVAPVYVAPGDGDAGAIDDDTFTGPASVTEVI"
     gene            7333..7641
                     /locus_tag="PL014_03805"
     CDS             7333..7641
                     /locus_tag="PL014_03805"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOT56245.1"
                     /translation="MSRLLSADEVATQLAGLPGWSRRDESLVATFEAPDFPAAIRLVE
                     AAADEAEQMNHHPDIDIRWRTTHWLLTTHDAGGLTQLDIELAHRISAAAAAQGASARD
                     "
     gene            7691..8362
                     /locus_tag="PL014_03810"
     CDS             7691..8362
                     /locus_tag="PL014_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HOT56246.1"
                     /translation="MAGRLNALRAGVLGANDGIVSTAGLVIGVAAATTDRGAVFTAGM
                     AGLAAGAMSMAVGEYVSVSTQRDTEKALIAKEIRELDEEPEAELNELAGIYAAKGLTP
                     DLARQVAEQLTAHDALGAHAEAELGIDPTQHANPWQAAWASMLAFTLGALLPLLAILL
                     PPLPWRVPVTVAAVVLALAATGWVSARLGDADARRATVRVVAGGLLAMAVTYGIGALV
                     GTQLG"
     gene            8369..9112
                     /locus_tag="PL014_03815"
     CDS             8369..9112
                     /locus_tag="PL014_03815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOT56247.1"
                     /translation="MSSRLLVIQHENDAPAAWLGQWWNDLGLELTVVRGHLGEAVPGP
                     GQWSGYDGLVVLGGAMGACDDAVAPWLAPTRDLIADAVAREVPTLGVCLGHQLASVAL
                     GGSVGRNPSGRTTGVVPVALTDAGASDPLLAGVDGALAVHYNDDVVLEVPTGATVLAT
                     LPDGRPQALRFGPSAWGVQFHPETSPEVFAAWLRWDSPDGTTPEQEALLAQVTAARED
                     LQSTWRVFAQRFAALLPAHPGVPASPVTS"
     gene            9195..10547
                     /locus_tag="PL014_03820"
     CDS             9195..10547
                     /locus_tag="PL014_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOT56248.1"
                     /translation="MSTSTSADPRERILILGAAGRDFHDFNVLYRDDPHSEVVAFTAT
                     QIPDIDGRTYPPALAGPHYPHGIPIVDESGLESLIARERVDTVVLAYSDLAHTQVMHL
                     ASRVVAAGADFVVPGARRTTLRSRRPVIAVTAVRTGVGKSQTTRYVAALLQSMGLRVV
                     AVRHPMPYGDLAAQTCQRFETHADLVRHDCTIEEREEYEPHIDAGVVVYAGVDYGAIL
                     REAEAEADVVLWDGGNNDLPFYVPDLHIVLADPLRPGDEAAFHPGEANVRMADLVIIA
                     KCDSATAGDIAAVEASVQALNPRAGVLRCDSPVTLDDPAAVTGRRVVVVEDGPTLTHG
                     SMSFGAGVVGAKDAGAATIVDPTPFAVGALAATYERYPNARGILPAMGYGEAQTRDLE
                     ATIRAMVDAGVVDAVVSGTPIDLTRVLTVDVPLVRARYSLREQEPGRIEAALRTTLGR
                     "
     gene            complement(10552..12702)
                     /gene="malQ"
                     /locus_tag="PL014_03825"
     CDS             complement(10552..12702)
                     /gene="malQ"
                     /locus_tag="PL014_03825"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOT56249.1"
                     /translation="MDPAAPSPRLVELAHAHGVATEYWDWQGQHVTVTAPAITAVLTA
                     LGVDSDGDEQVERALTEVDLAPWRRTLPATVVAREGWTPWVFAHVPAGTGIRLTVHLE
                     DGSVREVRQVDRWVPDRDVDGVAVGEATFELPGDLPLGWHRIVADVDAAPMDPRSVDA
                     TLVVTPHRLELPPALARGRAVGLMAQLYQVRSAGSWGVGDLGDLAELAAWAATEHDAD
                     FVLINPLHAAQPVAPMEPSPYLPTTRRFVNPLYLRVADVPETADLDDAASAKVAAFGA
                     VATALNSVDRINRDDAWAAKREALWIVFSAGRTAERDQAFAEFCAREGSGLTTFATWC
                     AIADDHGLPWTTWPEELQDPDSVAVALYREDHLEAVTFHQWLQWQLEEQLAGAQRTAQ
                     EAGMPLGIVHDLAVGVHPVGADAWGLGDALATGVTVGAPPDQFNQLGQDWSQPPWRPD
                     RLAELGYGPFRDMVRTVLRDSGGIRVDHIIGLFRLWWIPAGGTPADGTYVHYDHEALL
                     GILVLEAQRAGAVVIGEDLGVVAPHVRDYMTERGLIGTSILWFEWEGERPKPPEHYRD
                     LCLSTVTTHDLPPSAGYLLLEHIAIRETLGLLTRPVTEERAIEEASIARLRDACIERG
                     LLSPDAVADVDAVVAALHRWMALTPSRMVAVALADVVGDRRAINQPGTSDEYPNWRVP
                     LAGPDGEVVSLDDVRRADLAGPLFRALHGRPAHD"
     gene            complement(12734..>13171)
                     /locus_tag="PL014_03830"
     CDS             complement(12734..>13171)
                     /locus_tag="PL014_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT56250.1"
                     /translation="RPESVYSLGKHLEETMAIELCRWHPQLSVTALRFSNVMDVEDYA
                     EFPSFDADAALRRWNLWGYIDGRDGAQAVEKSLAYTVPGFDCFIIASPDTVMSRSNAD
                     LVAEQFPGVEVRGDLGEHDTLLSIDKARRILGYDPQHSWRGEV"
     assembly_gap    13172..13271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13382..14197
                     /locus_tag="PL014_03835"
     CDS             13382..14197
                     /locus_tag="PL014_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT56251.1"
                     /translation="MHTIVFLHAHPDDEASQTSGSMARAVAEGSRVVVVFATNGDHGE
                     VPADLAPGEGVVDRRRVEAAASAAAIGLHRVEWLGYADSGMSGWEQNSHEDAFVAAPL
                     EEAAARFAAILDEEDADIVVGYDWHGGYGHPDHVRVHEVLHAAAALAARRPRVLESTM
                     NRDAMRRLAEGARAAGAGAGADDIFDPDQPMDDGNPLGEPESAIHWRVDVSDRLDQRR
                     AALQAHASQTSDVGMMLSMPPEIFSVFFGHEHYIEPGREPGMVDGWPFEAQPA"
     gene            complement(14203..15132)
                     /locus_tag="PL014_03840"
     CDS             complement(14203..15132)
                     /locus_tag="PL014_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT56252.1"
                     /translation="MEDTDVTTATAPTPDSAPAGAVLAALDGSPADGPILDWAADEAA
                     RLGAQLRLVTAVDPGIQLTPYDAIVTGAPSLSEQLEADAQRLLEAAAERVRARHPGLH
                     VATSAPWGPAAASVVRMAEGARLVVVGAPARGLLERILLGSVALPVVAHAPCPAAVIP
                     ADTEVMPPRAIVVGVDGSERSGRAVALAAEMAAASGAALTAVIAWHLEVVDGVVVTEP
                     GTPRWDAVEQRYAARAHQVVDPVAARHPGVTMDVRVVHGTAPVALHDVADELDADRVV
                     VGSRGVGGFRGLLLGSVSRRVVEQAGRVVVVSR"
     gene            15220..16362
                     /locus_tag="PL014_03845"
     CDS             15220..16362
                     /locus_tag="PL014_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOT56253.1"
                     /translation="MATDPAQPLSPSDRARAAEAGVTRWLTGRMPPTPADELRDLADV
                     VADLGAGTGSGVGADAGAWDRYGDHGPVAALEARLADLLGKPAVAVFPSGIMAQQSML
                     RVWCDTRGSRRVAIPALSHLLRSEDDGPRLLHGFRFEHLTDGPSVPGVDDLAAIPGRL
                     GAALLELPLRDAGFLLPRWDDLVAFSQACRDRGVPLHLDGARIWESAPALQHSPAQIA
                     ALADSVYVSFYKGLGGLAGAALAGPVDEIAEARRWRTRMGGTLFTLLPYAVAALRGLE
                     QELPRMAEYHERAVTIAAGLQAHGIRVSPSPPHTTAFRIHVERDVEDCTQRRLRAMED
                     ERVALTPRWAASEVPGWSWTEFGVGPASMAWEVEEIIDTLVRVFGR"
     gene            16385..17317
                     /locus_tag="PL014_03850"
     CDS             16385..17317
                     /locus_tag="PL014_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT56254.1"
                     /translation="MTHPSPRERTQPVDLTRFAWLAIAAAVLTIALKTIAWQITGSVG
                     LLSDAAESVVNLVAAIVALVALRVAIMPADKNHHFGHSKAEYFSAALEGLMIFIAAAV
                     ILVTSIERFLNPRPLENVGIGLAVSVVASVVNGLVAMVLLRAGAKHRSITLTADGKHL
                     MTDVWTSVGVVVGVLLVAVTGWERLDPVVAFAVGLNILITGWRLIRSASAGLMDVALP
                     KEDNDVIREVLGRFTTDEVTWHRLMTREAGHRRFMQFDLLVPGAWSVQRGHDLIEDIT
                     TALDAEFPDIDVVCHLEPIEDPRSYEDAADRPRE"
     gene            17364..17792
                     /locus_tag="PL014_03855"
     CDS             17364..17792
                     /locus_tag="PL014_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT56255.1"
                     /translation="MTHLVVHFEIHASEPQVLIDFYGELFGWTFSRFGEVPYWIIDTG
                     EGAAGVDTPGHGINGGLTQREGPAPQPGAPINGCNIVVGIETGVDEVFARALALGASE
                     ASPLEDMEGVGRGGYLFDPDGNLFGLMSAVMSDGTNAMAG"
     gene            complement(17789..18484)
                     /locus_tag="PL014_03860"
     CDS             complement(17789..18484)
                     /locus_tag="PL014_03860"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT56256.1"
                     /translation="MAEVLSMLDAPLLAEHHCWFGGGTAIVLANGEFRESLDIDFLVS
                     DQQSYRQLRQIVRDHGLGGLTTRELVLGRPPTVDVYGIRSSVLVAGAAIKFEIIHEGR
                     IDLDNPSPGTEICGVRTLTRTDQVASKLLANDDRWADTSTFSRDLIDLAMMKPDAVAL
                     KAGARKAVDAYGTSVGTSLDKAVAHLRDRPQRLDACIRALKIDAPRATVWQRIRDLSA
                     RSAKVEGLGQNWR"
     gene            complement(18504..18965)
                     /locus_tag="PL014_03865"
     CDS             complement(18504..18965)
                     /locus_tag="PL014_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT56257.1"
                     /translation="MPASSPYTSPEQQADLERLSGRLRARRKALGVTAVACAEAAGVS
                     RVTLHRIEAGSPSVTIGAYINVAAALGLHLVVPILDAEGAGSPTITVGEYPGLRMLAW
                     QTDAGVTITESEALNLYERGWRHLDQEALTDHEKAFIQHLADTYSNGRLLV"
     gene            19398..19655
                     /locus_tag="PL014_03870"
     CDS             19398..19655
                     /locus_tag="PL014_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOT56258.1"
                     /translation="MKTMSYTESRARYAEVLDGVANDREEVIITRAGHDPVVIVSLED
                     YESLRETAYLMRSPANARRLLDAMERLEAGRGEPHDMIDAD"
     gene            19657..19914
                     /locus_tag="PL014_03875"
     CDS             19657..19914
                     /locus_tag="PL014_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOT56259.1"
                     /translation="MLLVWDENAWQDYLWWQAQDRRVLKRINTLLADIERNGNEGIGK
                     PEALKHDFAGYWSRRITDEHRLVYKLVGQEVRIAACRYHYG"
BASE COUNT         2931 a         7137 c         6823 g         2937 t
ORIGIN      
        1 gccgtgacct tggcgccctt gacgcggccg gccatggggc tggtgttgat cccgatcgtc
       61 atggagatgg cgggctcgtc gacggtgatg agcggcaggg ggcgcgggtc gtcgacatcg
      121 gcgagggtct cgccgatggt gatctcgggg atgccggcga cggcgatgat gtcgccgggg
      181 cccgcgctct cggccggctt gcgctcgagg gcgtcggtca tcagcagttc ggtgatcttg
      241 accttctcga cggaaccgtc acggcggcac cacgccacct gctggccctt gcggatggtg
      301 ccgttgtgga cccgcaacag ggccaggcgc ccgaggaagt tcgaggcgtc gaggttggtg
      361 acgtgcgcct ggagcggcgc gtcggagtcg taggacgggg ccgggatggt ctcgatgatc
      421 gtgcggaaca gcggctcgag gtcctcaccg tcgggcaggc ccccgtcggc cgggcggttc
      481 atggacgcgc ggccggcctt ggccgaggcg tagacgatcg ggaactcgag ctggtcctgg
      541 tggtgctcgg cgtcgaccat gaggtccatg aagagctcgt agacctcgtc gacgacctcg
      601 gagatgcggg cgtcggggcg gtcgaccttg ttgatgcaca ggacgaccgg catcttcagg
      661 gcgagcgcct tgcgcagcac gaagcgggtc tggggcagcg gaccctcgct ggcgtcgacg
      721 agcagcacga caccgtcgac catcgacagg ccgcgctcga cctcgccacc gaagtcggcg
      781 tggccggggg tgtcgatgat gttgatcgtc atgccgtcgg tgagcccggc gtcgagcgcg
      841 gccttgcctg cgtagtgcac ggcggtgttc ttcgcgagga tggtgatgcc cttctcacgc
      901 tcgaggtcac cactgtccat cgcgcggtcg gcgatgtggt cgtgttcacc gaaggccccc
      961 gactgccaga gcatcttgtc gaccagggtg gtctttccgt ggtcgacgtg ggcgacgatg
     1021 gcgacgttac ggagatcagc gcgacgggat acaggcataa ccaccagtct ctcaggtagt
     1081 tcgccaccga ccgaatcgag tgcagcgcgc acggtctggt gcaggatctg cgcgcagatc
     1141 aggtctcact tggataacca ctggcgcaag gacgggtccg caggccgttt ttgccgttat
     1201 ggtcaccggc acggagggtg gggagactca tcacctcgcc tgccgggccc aaaactcgac
     1261 cgcccacccg ggcgacccga ggttcgcata accccaagcg aggttgatcg atgaagctca
     1321 agcggaaggc gccagttgtc ctgttgacgg cggccgccct caccgtgacc ggctgcgccc
     1381 agtcagaccg tgagtcggag tccaccgaca ccggcacggc cagcggcgag ttcaaggaca
     1441 cgttcacgtt cggcgccgcc ggcgcaccga agttgttcga cccgttctac gccacggacg
     1501 gtgagacgtt ccgggtcacc cgccagatgc accagggtct gctcggcgtc aagccgggta
     1561 ccgccgacgt ccagcccgaa ctggccgaga gctggacccc ctccgcagac ggcctgacct
     1621 gggagttcaa gctcaagcag ggggtgaagt tctccgacgg cgagccgttc aacgccgagg
     1681 ccgtctgcgc gaacttcacc cgcatgtacg agtccaaggg cgccgcgcag accgcagccg
     1741 agtactgggg ctacttcttc ggctcgttca gcgacaagcc cgagggctcg ctgtacaagt
     1801 cgtgcgaggc caaggacgag tccaccgcgg tcatcggcat cacccgcgcg acgtcgagct
     1861 tcccgacgat cctgtcgctc gactcgttct cgatgcagtc ccccaaggcc atggccgccg
     1921 gggatgccgg gaacgtccag gcagcgggcg agggcttcac ctacccgccc tacgtcatgg
     1981 cccccgtcgg catcggcccc tacaagctcg acaagtacga cgaggccaac aagacggtca
     2041 cgctcgtcgc caacgacatg ttctacgggg agaagccgaa gacggccaag ctcgtcttca
     2101 agatcatccc cgacgagagc acccgccgcc aggagctgca ggccggctcg atcgacggct
     2161 acgacctgcc caacccggtg gactggaagg gtcttgagga cgagggcaac agcgtcgagg
     2221 tccgcccggc gttcaacatc ctctacatgg gtctgaacgc gaagaagaac cccgtgctca
     2281 aggacctcaa ggttcgccag gcactctccc acgcgatcaa ccgtgaccag atggtcaaga
     2341 cgcagctgcc cgagggtgcg caggccgcga gccagttcat gcccgacacg gtctccggct
     2401 acaacacctc gttgcagccc gacgcctatg acccggagaa ggccaaggcc ctgctcaagg
     2461 aggccggcgc cgagggcatg accctgcagt tcgccttccc gaccgaggtc tcgcgtccgt
     2521 acatgccgga cccgcagaag atccacgacg ccttcaagaa ggacctcgag gccgtgggca
     2581 tcaaggtcga tgtcgtcagc aagccgtgga acggcggcta cctggacgac gccagcgcgg
     2641 gcaactacga cgcctggctg ctcggctgga ccggtgacta caacgccgcg gacaacttcc
     2701 tgggcacgtt cttcagcaac tacgagcaga acgacttcca gacaagcgcc tacccgtggg
     2761 gcaagaagct gtccgaggac ctcaaggccg cggacgcgat cgtcgacgag gcggcgcgca
     2821 aggctgccta cgagcagctc aacaagcaga tcgcgcagga gtacatcccg ggcctgccga
     2881 tcagccactc gccgccggcg ctcgtcgtga gctcgaaggt tcagggtctc gtcgcgagcc
     2941 cactgaccgc cgagatgttc gaccaggtca cggtcagcag caagtgagtc agggcgactg
     3001 aaacacgtcg ttcatcccgt gcggtggccg cctcgaacca ctagggtccg aggcggccac
     3061 tgctgtctca ccggagcatc aaggagcccc cgttggcgag attcatcatc agacgcctca
     3121 tccagatggt cggggtggtg ttcgtgctct cgctgctgct cttcctgtgg ctgcggtcac
     3181 tccccggcgg gccggtgtcc gccatcctcg gcgagcgcgc cacggacgag tcacgagccg
     3241 ccctcgagaa ggcgttcggc ctcgaccaac cggtgtacgt gcagtacctg aagttcctcc
     3301 agcgcgcctc ccagggtgac ttcggcgcct cgacggccgt ccttcccgga cgtgacgcgc
     3361 tcgagatctt cttcacccgg cttccggcga ccatcgaact gagcatcatc gcgatggcga
     3421 tcgccatcgt gctcgcgatc ccgctggggt acgtggcagc caagcgccac gggtcgagca
     3481 tcgacaacgg cgccgtggtg ctctccctca tcggggtcgc cgtgcccgtc ttcttcctcg
     3541 ccttcctcct caagtactgg ttcgccatca agacccagac cttccccgtc tctgggcgtc
     3601 aggatgccgc cctgggctgc acccgcgtga cgaacttctt cgtcctggac ggcttgctca
     3661 cccgggagtt cgactgctcc gccgacgcgc tgcgccacct cgtgctcccc gcgatcgctc
     3721 tcgcgaccat cccgttcgcc gtcatcttcc ggatcacccg cgcgtccgtg ctcgacgtcc
     3781 tcgacgagga ctacgtgcgc accgcggagg ccaagggcct gaccgcccag gtcatccgag
     3841 cccgacacgt catgcgcaac gccctgctcc ccgtcgtcac gacgatcggc ctgcagaccg
     3901 gcggcctgct cgccggcgcc gtgctcaccg agacggtgtt caacttcggc ggtatcggct
     3961 ccaccctcgc ccaggccttc acccagcgcg actacgcggt gctccaggtg ctgatcctgg
     4021 ccaccgccac gatctacgtc ctggtcaacc tgctcgtcga catctcctac gcgatcatcg
     4081 acccgagggt gcgtgcccga tgagcccccg caaccccatg aacgcccgca agcagcggat
     4141 cgacgacctc gccgcctcga gcgtgatcgt cgacgacgcc ggcgccgtcg acgaggcccc
     4201 cggagtctcc ctcatcgcga gcgcatggcg tcgccttcgt cgcgacccgg tgttcctcct
     4261 cggtgccacc atcacgggcc tgttcatcat cgtcgcgatc atcgcgccgt ggatcgcacc
     4321 gcacgacccc gacgcccgcc tgctcatcga ccaggtcagc cggcagtcca accccatccc
     4381 ggggcctcag gacgggttcc ccctcggtgc ggacgaccgg gggcgcgacc tgctctcccg
     4441 cctcatcgtc ggcagccgcc agaccctgct cgtcggcgtc gcggcaaccc tgttgggcct
     4501 gttcggtggt ctcgtcctcg gcatcctcgc cggcgccttc ggtggtgccg tcgacgcgtt
     4561 cgtcatgcgc gcggtcgacg tcatgctctc gatcccctcg ctcctgctcg ccgtctccat
     4621 cgcggcgctg gcacgtcagc ccagccagtg gacggtcatc atcgccatcg cggtggtgca
     4681 gatcccggtc ttcgccaggc tcctgcgcgg ctcgatgctg gcgcagcggt ccagcgatca
     4741 cgtgctcgcc gccagagcac tcggcgtcaa gcgcagcgcc atcatcttcc gccacatggt
     4801 gccgaactcg ctggggccgg tcatcgtcca gtcgaccctg gtgctggcca cctccatcat
     4861 cgacgccgcc gcgctgtcct tcctcggtct cggcaacccc gacgacgcca agcccgagtg
     4921 gggccagatg ctcggacagg cgcaggtgta catccaggac tacccgcacc tggccgtcta
     4981 cccggccctg tgcatcgtct tcgtcgccct cggcttcacc ctgatgggcg agtcgttgcg
     5041 cgaggccctc gaccccaaga gcaggaggtg acgaggtgac cgccacggca tcccctcccc
     5101 ccacgaccac ccgcggtgac gcgccgctgc tgtcggtccg tgacctgcgg gtgacgttca
     5161 cccgccgcgg cgagaagccg ttcatggccg tcgacggggt gagcttcgac gtgcgccccg
     5221 gccagacggt cggcctcgtc ggcgaatccg gctgcggcaa gtcggtcacg tcgctggcca
     5281 tcatgggtct gctgcccaag cgcggcaaca ccgtcgaggg tgaggcctcc ttcgagggcg
     5341 tcaacctgct gaccctggac gagaaggcca agcgcgaacg ccgcggacgc gacatcgcga
     5401 tgatcttcca ggacccgttg tcgtccctga accccgtcgt cccggtgggg ctccaggtca
     5461 ccgaggtgct cgagcggcac cgcggcatgt ctcgcaaggc cgccacgccc gtggcccgcg
     5521 acctgctgaa gaaggtcggc atccccgatc cggaccgtcg cctgaaggac tacccccacc
     5581 agatgagtgg cggaatgcgg cagcgggcgc tcatcgccat ggcggtcgcg tgcgcaccac
     5641 gactgctcat cgccgacgag ccgacgacgg ccctcgacgt gacgatccag gcgcagatcc
     5701 tcgcgctgct caaggaactc gtccaggaca ccggcaccgc cctcgtgatg atcacccacg
     5761 acctcggcgt cgtcgccggg ctgtgcgacg aggtcaacgt cctgtacgcc ggccgcatcg
     5821 tcgagcgcgg cgagcgccac gagctgttcg ccgacccccg gcatccgtac accaacggcc
     5881 tgctggcctc gattccccgc ctcgacgccc cgcgcggcga gcggttgacg ccgatccccg
     5941 ggtcggtggc cgacaacctg ccgtgggaca gcgcctgtgc gttcgcgccc cggtgctccc
     6001 agccggtcga cgcctgcgtc cacaccaccc cggccctcga cgagaccggc cgccggtccc
     6061 tgcgctgcca caaccccgtg aggtgacgtg atgaccgaca cgacgaccca ggcaacccct
     6121 ccctccggcg acgaacgcga cgtgctcgtc gacgtccgcg gcgtcaaggt gcacttcccc
     6181 atcaagcggg gcgtcatctt cgacaaggtg gtcggccacg tgtacgccgt cgacggcgtc
     6241 gacctccaga tccgccgcgg cgagacctac ggcctcgtcg gtgagtcggg ctgcggcaag
     6301 tccaccctcg gcaaggcgat cctcaacctc gaaccaccga ccgagggcag cgtcatcctc
     6361 gacggcaccg acatcgcatc cctgcgcggg gaggagctgc gtcggcgccg caaggacatc
     6421 cagatggtct tccaggaccc gatgagcagc ctggaccccc gccagtcggt ggagtcactg
     6481 ctcgtcgagg gcatgcgtgc ccacggcctg gacaccgacg gggcgagcac caagaagcgg
     6541 ctgcgggagc tgctcgcggc cgtcgggctc cctgctgcgg ccctgacgaa gtacccgcac
     6601 gagttctccg gtggccaacg ccagcgcatc ggcatcgccc gggcgctgtc cgtcgagccc
     6661 aagctcatcg tcgccgacga accggtgagc gccctcgacg tctcggtgca ggcccaggtc
     6721 atcaacctcc tcgaggagct gcaggacgag ttcgacctca cctacctcgt cgtcgcgcac
     6781 gacctcgccg tcgtgcggca catcagcgac cggatcgggg tcatgtacct cggtgggctc
     6841 gtcgaggagg ccggcgccga cgacctctac ggccagcccc tgcatccgta cacgcgcgcg
     6901 ctgctgtcgg ccgtgccggt accggacccg accatcgagg acacccgtga gcagatcctg
     6961 ctgacgggcg acctgccctc gccgtccaac ccgccacccg ggtgccggtt ccacacccgc
     7021 tgcccgtggc gccaggagac ccgctgcgac gacgagcggc ccccgctgcg cgtggtgcag
     7081 atcgacggcg tcgaccccag ccaccgggtg gcctgccact gggtcgagca gatcgccagc
     7141 ggcgagatcc gtccgcacga ggtggcaccg gtctacgtcg ctccgggtga cggcgacgcc
     7201 ggcgccatcg acgacgacac cttcaccggc ccggcatccg tgaccgaggt catctgaccc
     7261 gcccggcgca cgtcggatgc cgcccgcgcc ccccgcgcgg acggcatccg tcgtcgggtc
     7321 cacaatggcc ccatgagccg actcctttcc gctgacgagg tcgccaccca gctggccggg
     7381 ctgcccgggt ggtcccggcg cgacgagagc ctggtcgcca cgttcgaggc acccgacttc
     7441 cccgcggcga tccgcctcgt cgaggccgct gccgacgagg ccgagcagat gaaccaccac
     7501 ccggacatcg acatccgctg gcgcaccacg cactggctgc tgacgaccca cgacgccgga
     7561 ggcctgaccc agctcgacat cgagctggcc caccggatct cggcggccgc ggcggctcag
     7621 ggggcgtccg ctcgtgactg agacctcgcc gggcacggca cccgagcaca ccgaggccca
     7681 cgacgggtcg atcgccggcc gtctcaacgc gttgcgggct ggagtcctcg gcgcgaacga
     7741 cggcatcgtg tccaccgctg gcctcgtcat cggggtggcc gcggcgacca ccgaccgcgg
     7801 cgccgtgttc accgcgggca tggccgggct cgccgccggc gcgatgagca tggccgtcgg
     7861 cgagtacgtg tcggtgagca cgcagcgcga caccgagaag gccctgatcg ccaaggagat
     7921 ccgagagctc gacgaggagc ccgaggccga actgaacgaa ctggccggca tctacgccgc
     7981 caagggactg acccccgacc tggcccgcca ggtggccgaa cagctgaccg cccacgacgc
     8041 cctgggcgcc cacgccgagg ccgagctggg gatcgacccg acccagcacg ccaacccgtg
     8101 gcaggcggcc tgggcgtcga tgctcgcctt caccctgggg gccctgctcc ccctgctggc
     8161 catcctgctc ccgcccctgc cgtggagggt gcccgtcacc gtcgcggccg tggtgctcgc
     8221 actggccgcc acgggctggg tcagcgcccg cctcggcgat gccgacgcgc gccgcgcgac
     8281 ggtgcgcgtg gtcgccgggg gcctgctcgc catggcggtc acctacggca tcggagcgtt
     8341 ggtcggcacg cagctaggct gacccgccat gagctctcga ctgctcgtga tccagcacga
     8401 gaacgacgcc ccggcggcct ggctcgggca gtggtggaac gacctcggcc tggagctgac
     8461 ggtcgttcgc ggccacctcg gcgaggccgt gccgggcccg gggcagtggt ccgggtacga
     8521 cggcctcgtc gtcctcggcg gggcgatggg cgcctgcgac gacgccgtcg cgccgtggct
     8581 ggcccccacc cgcgacctca tcgcggatgc cgtggcgcgc gaggtcccca ccctcggggt
     8641 gtgcctgggc caccagctgg cctcggtggc cctcggtggg tcggtgggac gcaacccgtc
     8701 gggccgcacc accggtgtcg tgccggtggc cctcaccgat gccggggcga gcgacccgct
     8761 cctcgcgggc gtcgacggcg cgctggcggt gcactacaac gacgacgtcg tgctcgaggt
     8821 gcccaccgga gccaccgtgc tcgccaccct ccccgacggg cggccccagg ccctgcgctt
     8881 tgggccgagc gcctgggggg tgcagttcca cccggagacc tcccccgagg tgttcgcggc
     8941 ctggctgcgc tgggactcac ccgacggcac cacccccgag caggaggccc tgctcgccca
     9001 ggtgacggct gcccgcgagg acctgcagag cacctggcgg gtgttcgcac agcgcttcgc
     9061 cgccctgctc cccgcgcacc cgggcgtgcc tgcgtctccc gtcacgtcct gagtcccccg
     9121 ccgccgcacg ggagtaacgt cggcaccggc gggtcccgca ccccgcggtc gacccagccg
     9181 tgacggagca cgccatgagc acctcgacct cggcggatcc tcgcgaacgc atcctcatcc
     9241 tgggtgctgc gggacgcgac ttccacgact tcaacgtcct gtaccgcgac gacccccact
     9301 ccgaggtcgt cgcgttcacc gcgacgcaga ttcccgacat cgacggccgc acctacccgc
     9361 ccgcgctcgc cgggccgcac tatccgcacg gcatcccgat cgtcgacgag tccggtctgg
     9421 agtcgctcat cgcgcgtgag cgggtggaca ccgtggtgct cgcctactcc gacctggcgc
     9481 acacccaggt catgcacctc gcctcgcgcg tggtcgcggc aggtgccgac ttcgtcgtgc
     9541 cgggcgcccg ccgcacgacg ctgcgctcgc gtcgcccggt catcgccgtg accgccgtgc
     9601 gcaccggggt gggcaagagc cagaccaccc gctacgtggc cgcgttgttg cagtcgatgg
     9661 ggctgcgtgt cgtggcggtc cgccacccga tgccgtacgg cgacctcgct gcgcagacct
     9721 gccaacgctt cgagacccac gcggacctcg tgcggcacga ctgcaccatc gaggaacgcg
     9781 aggagtacga gccgcacatc gacgccgggg tcgtcgtcta cgccggggtc gactacgggg
     9841 ccatcctgcg cgaggccgag gccgaggcgg acgtcgtgct gtgggacggc ggcaacaacg
     9901 acctgccgtt ctacgtcccc gacctccaca tcgtgctcgc cgacccgcta cggcccggtg
     9961 acgaggccgc cttccaccct ggtgaggcga acgtgcggat ggccgacctg gtcatcatcg
    10021 cgaagtgcga ctcggcgacc gccggggaca tcgcggccgt cgaggcatcg gtgcaggcgc
    10081 tgaacccgag ggccggggtg ctgcgctgcg acagcccggt gaccctcgac gacccggcag
    10141 cggtcaccgg gcgacgggtc gtcgtcgtcg aggacggccc caccctcacc cacgggtcga
    10201 tgtccttcgg tgccggtgtg gtcggcgcga aggatgccgg ggcggccacg atcgtcgatc
    10261 cgacgccgtt cgcggtgggc gcgttggcgg cgacgtacga gcgctacccc aacgcgcgcg
    10321 gcatcctgcc ggccatgggc tacggcgagg cccagacccg cgacctcgag gccacgatcc
    10381 gcgccatggt cgacgccggc gtcgtcgacg cagtcgtgtc gggcacgccc atcgacctga
    10441 cccgggtcct caccgtcgac gtcccgctcg tgcgggcccg ttactcgttg cgcgagcagg
    10501 agcccggccg gatcgaggcg gcgctgcgca cgaccctggg tcgctgacgg ctcagtcgtg
    10561 ggccggccgg ccgtgcaggg cgcggaacag gggccctgcc aggtccgcgc ggcggacgtc
    10621 gtccagcgag acgacctccc cgtcgggccc ggcgagcggc acccgccagt tcgggtactc
    10681 gtcgctggtc ccgggctggt tgatcgcccg ccggtccccc accacgtccg cgagcgcgac
    10741 ggccaccatg cggctcggtg tgagcgccat ccaccggtgc aacgcggcga cgacggcgtc
    10801 gacgtcagcc acggcatccg gcgacagcaa cccgcgctcg atgcaggcgt cgcgcagccg
    10861 ggcgatggag gcctcctcga tcgcccgctc ctccgtgacc gggcgggtca gcagccccag
    10921 tgtctcgcgg atcgcgatgt gctcgagcag caggtagccc gctgacggcg gcaggtcgtg
    10981 cgtcgtcacg gtcgacaggc acaggtcgcg gtagtgctcg ggcggcttgg gccgctcacc
    11041 ctcccactcg aaccacagga tcgaggtgcc gatcaacccc cgctcggtca tgtagtcgcg
    11101 cacgtgcggg gcgacgacgc cgaggtcctc tccgatgacg acggccccgg cccgctgcgc
    11161 ctccagcacg aggatgccga gcagcgcctc gtggtcgtag tgcacgtagg tgccgtcagc
    11221 gggggtgccg cccgcaggga tccaccagag ccggaacagg ccgatgatgt ggtcgacccg
    11281 gatgccgccg gagtcgcgca ggacggtgcg caccatgtcg cggaacgggc cgtagcccag
    11341 ctccgcgagc cggtcgggac gccatggcgg ctggctccag tcctgaccga gctggttgaa
    11401 ctggtcgggc ggggcgccga cggtgacccc cgtggcgagt gcgtcgccca ggccccaggc
    11461 gtcggccccg accgggtgca cccccacggc gaggtcgtgc acgatgccga gcggcatccc
    11521 ggcctcttgg gccgtgcgct gggcgccggc cagctgctcc tcgagctgcc actgcagcca
    11581 ctggtggaag gtcaccgcct cgaggtggtc ctcgcggtag agggcgaccg cgacggagtc
    11641 ggggtcctgg agctcctcgg gccaggtcgt ccacggcagc ccgtggtcgt cggcgatcgc
    11701 gcaccacgtc gcgaaggtcg tgaggccgct gccctcgcgc gcgcagaact cggcgaaggc
    11761 ctggtcgcgc tccgccgtgc ggccggcgct gaacacgatc cacagggcct cgcgcttcgc
    11821 ggcccaggcg tcgtcgcggt tgatccggtc gacggagttc agcgccgtcg ccaccgcacc
    11881 gaacgcggcg accttcgcgg atgccgcgtc gtcgaggtcg gcggtctcgg gcacgtccgc
    11941 gacccgcagg tagagggggt tgacgaagcg ccgcgtggtc ggcagatagg gcgagggctc
    12001 catcggcgcc accggctgcg cggcgtgcag ggggttgatc aggacgaagt cggcgtcgtg
    12061 ctcggtggcg gcccaggcgg cgagctcagc gaggtcgccg aggtccccca ccccccagga
    12121 gccggcggag cggacctggt agagctgcgc catcaggccc acggcacggc cccgggccag
    12181 tgcggggggc agctcgagac ggtggggggt gaccacgagc gtggcatcga cgctgcgcgg
    12241 gtccatcggc gcggcatcca cgtcggccac gatgcggtgc cacccgagcg gcaggtcccc
    12301 gggaagctcg aaggtggcct cgccgacggc gacgccgtcg acgtcgcggt ccggcaccca
    12361 ccggtccacc tggcgcacct cgcgcaccga gccgtcctcg aggtggaccg tgaggcggat
    12421 gccggtgccc gccggcacgt gggcgaacac ccacggggtc cacccctcac gggccaccac
    12481 cgtggcgggg agggtgcgcc gccacggggc gaggtcgacc tcggtcagcg cccgttcgac
    12541 ctgctcgtcg ccgtcgctgt cgaccccgag cgcggtgagc acggcggtga tcgccggggc
    12601 cgtcaccgtg acgtgctggc cctgccagtc ccagtactcc gtcgcgacgc cgtgggcgtg
    12661 ggcgagctcg acgagtcggg gcgacggtgc ggcggggtcc atggccccat cctgccaagc
    12721 cggggcgggt gggtcagacc tcgccccgcc acgagtgttg cgggtcgtag ccgagaatcc
    12781 gtcgcgcctt gtcgatcgac aggagggtgt cgtgctcccc gaggtcaccg cggacctcga
    12841 cacccgggaa ctgctcggcg acgaggtcgg cgttgctgcg gctcatcacg gtgtcgggcg
    12901 aggcgatgat gaagcagtcg aagcccggca cggtgtacgc caacgacttc tccacggcct
    12961 gcgcaccgtc gcggccgtcg atgtagcccc agaggttcca gcggcgcagc gccgcatcgg
    13021 cgtcgaagga cgggaactcg gcgtagtcct cgacgtccat gacgttcgag aaccgcagcg
    13081 cggtgaccga cagctgcggg tgccaccggc acagctcgat cgccatcgtc tcctcgaggt
    13141 gcttgccgag ggagtagacg gactcgggac gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nagccccgca ggtgctccac gacgtgccga ccgagcttgc cggatcctcc
    13321 ggtgaccgcg atgcgcatgg aatggagcct aggcgctcgc gatggtgtgc acactgtccc
    13381 ggtgcacacc atcgtcttcc tccacgccca ccccgacgac gaggcctccc agacctccgg
    13441 ctcgatggct cgcgccgtcg ccgagggcag ccgcgtcgtc gtcgtcttcg cgaccaacgg
    13501 cgaccacggc gaggtcccgg ccgacctcgc ccccggcgag ggcgtggtcg accgccgacg
    13561 ggtcgaggca gcggcatccg ccgcggcgat cgggctgcac cgcgtcgagt ggctcggcta
    13621 cgccgactcg ggcatgagcg ggtgggagca gaactcccac gaggacgcct tcgtcgccgc
    13681 cccgctcgag gaggccgccg cacggttcgc cgccatcctc gacgaggagg acgccgacat
    13741 cgtcgtcggc tacgactggc acggcggcta cggccacccg gaccacgtcc gcgtccacga
    13801 ggtgctgcac gccgccgcgg ccctggcagc acgacggccg cgggtgctgg agtcgacgat
    13861 gaaccgtgac gcgatgcgcc gcctcgccga gggtgcgcgg gccgcgggcg cgggcgcggg
    13921 cgctgacgac atcttcgacc cggaccaacc gatggatgac gggaacccgc tcggcgagcc
    13981 ggaatcggcc atccactggc gggtcgacgt cagcgaccgg ctcgaccagc gccgcgcggc
    14041 cctccaggcg cacgcgagcc agaccagcga cgtcgggatg atgctgtcga tgccgccgga
    14101 gatcttcagc gtgttcttcg gacacgagca ctacatcgag ccgggccgcg agccgggcat
    14161 ggtcgacggc tggcccttcg aggcgcagcc cgcctgacgg ggtcagcgcg agacgacgac
    14221 cacgcgcccg gcctgctcga cgacgcggcg gctcaccgat ccgaggagca gcccccggaa
    14281 cccaccgacg ccacggctgc cgaccaccac ccggtcggcg tcgagctcgt cggcgacgtc
    14341 gtgcagagcc acgggcgccg tgccgtgcac gacgcgcacg tccatcgtca cgccggggtg
    14401 cctcgcagcg accgggtcga ccacctggtg cgcgcgcgcc gcataccgct gctccacggc
    14461 atcccaccgc ggcgtcccgg gctcggtgac gaccacgccg tcgacgacct cgaggtgcca
    14521 tgcgatgacg gccgtgagcg ccgcgcccga ggcggcggcc atctccgcgg cgagtgccac
    14581 ggcgcggcca ctgcgctcgc tgccgtcaac accgacgacg atggctctcg gcggcatgac
    14641 ctccgtgtcg gcggggatga cggcagccgg gcacggggcg tgggcgacga cgggcagggc
    14701 cacggacccc agcaggatac gttccagcag gcctcgggcc ggggccccga cgacgacgag
    14761 gcgagcgccc tcggccatcc gcacgaccga ggcggccgcg ggtccccacg gcgccgaggt
    14821 ggcgacgtgc agccccgggt ggcgagcacg aacacgctcg gccgctgcct cgagcagtct
    14881 ctgggcgtcg gcctcgagct gttcggacag gctcggtgcg ccggtgacga tggcgtcgta
    14941 cggcgtgagc tggatgccgg ggtcgacggc cgtgaccagc cgcagctgag cgcccagacg
    15001 ggcggcctcg tcagccgccc agtcaaggat cggcccgtcg gccggggagc cgtcgagggc
    15061 ggcgagaacg gcgccggcgg gggcgctgtc cggggtcggg gcggtggcgg tggtgacgtc
    15121 ggtgtcctcc atgaccccat cgtcctcctc gctggggctg ggcgcacccg tctgcgcccg
    15181 aggtgttggc cacaccccgg gcccgggcag gatggcgtca tggccaccga ccccgcgcag
    15241 cccctgtcac cttctgatcg tgcgcgcgcc gccgaggcgg gggtgacccg ctggctcacc
    15301 ggccggatgc cgcccactcc cgccgacgag ctgcgtgacc tcgccgacgt ggtcgccgac
    15361 ctgggcgcgg ggacggggtc aggcgtgggg gccgatgccg gcgcgtggga ccgctacggc
    15421 gaccacggcc cggtggccgc actcgaggcc cgcctcgcgg acctgctcgg caagccggcg
    15481 gtggctgtct tccccagcgg gatcatggcg cagcagtcga tgctgcgggt gtggtgcgac
    15541 acccgggggt cgcgacgcgt ggcgatcccg gcgctgtcgc accttctgcg cagcgaggac
    15601 gacggccccc ggctgctcca cgggttccgt ttcgaacacc tcaccgacgg gccgagcgtc
    15661 cccggtgtcg atgacctcgc ggcgatcccg gggcgcctgg gtgcggcgtt gctcgagctg
    15721 cccctgcgcg atgccgggtt cctcctgccc cgctgggacg acctcgtcgc cttcagccag
    15781 gcgtgccgcg accggggggt gccgctgcac ctcgacgggg cacgcatctg ggagtcggca
    15841 cctgcgttgc agcactcccc cgcgcagatc gcggccctgg ccgacagcgt ctacgtgtcg
    15901 ttctacaagg gcctcggcgg gctggccggg gccgcgctgg ccgggcccgt cgacgagatc
    15961 gccgaggcga gacggtggcg cacccggatg ggcggcaccc tgttcaccct gctgccgtac
    16021 gcggtcgcgg cgctgcgcgg cctggagcag gagctgccgc ggatggcgga gtatcacgag
    16081 cgggcggtga cgatcgccgc gggactgcag gcacacggca tccgggtcag tccgtctccg
    16141 ccgcacacga ccgcgtttcg catccacgtc gagcgcgacg tcgaggactg cacgcagcgg
    16201 cggttgcggg cgatggagga cgagcgcgtg gcgctgacgc cgcggtgggc ggcatccgag
    16261 gtgcccgggt ggtcgtggac cgagttcggt gtcgggccgg cgtcgatggc gtgggaggtc
    16321 gaggagatca tcgacaccct cgtacgggtc ttcgggcgct gaccgccggg aggacaatgt
    16381 ctgcgtgacg cacccgagcc cccgcgaacg cacccagccc gtcgacctca cccgcttcgc
    16441 gtggttggcc atcgcggcgg ccgtgctcac catcgccctg aagaccatcg cctggcagat
    16501 caccgggtcg gtcgggctcc tctcggatgc cgcggagtcg gtcgtcaacc tcgtcgccgc
    16561 catcgtggcg ctcgtggcac tgcgggtggc gatcatgccg gccgacaaga accaccactt
    16621 cgggcacagc aaggccgagt acttctccgc cgccctcgag gggctgatga tcttcatcgc
    16681 ggctgcggtg atcctcgtga cgtcgatcga gcggttcctc aacccgcgac cgctcgagaa
    16741 cgtcgggatc gggctcgcgg tgtcggtcgt ggcatcggtg gtcaacgggc tcgtcgcgat
    16801 ggtgctgctg cgggccgggg cgaagcaccg ctcgatcacc ctgacggccg acggcaagca
    16861 cctcatgacc gacgtgtgga cctcggtcgg cgtcgtcgtc ggggtgctgc tcgtcgcggt
    16921 caccgggtgg gagcggctcg acccggtcgt ggcgttcgct gtcgggctga acatcctcat
    16981 caccgggtgg cggctgatcc gcagcgcgtc ggcgggtctc atggacgtcg cccttccgaa
    17041 ggaggacaac gacgtgatcc gggaggttct cggccggttc accaccgacg aggtcacctg
    17101 gcaccggttg atgacccgcg aggccggtca ccgccgcttc atgcagttcg acctgctcgt
    17161 gccgggtgcc tggtcggtgc agcgcgggca cgacctcatc gaggacatca cgaccgcgct
    17221 ggatgccgag ttcccggaca tcgacgtcgt ctgccacctg gagccgatcg aggatccgcg
    17281 ctcctacgag gacgccgcgg accgcccccg cgagtaacgt ggccgctccc ggagcatcgc
    17341 cgctcccgcc ccgaggaggc gccatgaccc atctcgtcgt ccacttcgag atccacgcga
    17401 gcgaaccgca ggtcctcatc gacttctacg gtgagctctt cggttggacg ttctcacgct
    17461 tcggggaggt gccgtactgg atcatcgaca ccggcgaggg tgctgccggg gtcgacaccc
    17521 ccggccacgg catcaacggc gggttgaccc agagagaggg ccccgcaccg cagcccgggg
    17581 ctcccatcaa cgggtgcaac atcgtggtcg ggatcgagac cggcgtcgac gaggtcttcg
    17641 cgcgcgccct ggcgctgggg gcgagcgagg cgtcgccgct ggaggacatg gagggcgtcg
    17701 gccgtggcgg ctacctgttc gaccccgacg gcaacctgtt cgggctcatg tccgcggtga
    17761 tgtcggacgg caccaacgcc atggccggct agcgccagtt ttggcccaaa ccttcgacct
    17821 tcgcggatcg ggcggacagg tcgcggatcc tctgccagac ggttgcgcga ggtgcgtcga
    17881 tcttgagtgc ccggatgcat gcatcgagac gttgcgggcg atcccgcagg tgggcgactg
    17941 ccttgtcgag gctcgtgccg accgacgtgc cgtaggcatc gaccgccttc ctggcgccgg
    18001 ccttcagcgc gacggcgtcg ggtttcatca tggccaggtc gatgaggtcg cggctgaacg
    18061 tcgaggtgtc tgcccaacgg tcgtcattcg ccagcaattt gcttgcgacc tggtccgttc
    18121 tcgtgagggt ccgcacgccg cagatctcag ttcccgggga gggattgtcg aggtcgatgc
    18181 ggccttcatg gatgatctcg aacttgatcg cagcccccgc gacgaggacg gacgaccgga
    18241 tgccgtaaac gtcaaccgtg ggtggcctcc ccagcacgag ttcgcgcgtg gtgagcccgc
    18301 ccagaccatg gtccctgacg atctggcgaa gttgtcggta cgactgctgg tcggagacca
    18361 ggaagtcgat gtccaacgac tcgcggaact cgccgtttgc gaggacgatc gcggtgccgc
    18421 ccccgaacca acagtggtgc tctgccagga gcggtgcgtc gagcatcgac agcacctcgg
    18481 ccaccgcctg gtggtggtgc cgcctaaaca aggagcctgc cgttgctgta ggtgtctgcg
    18541 aggtgctgga tgaacgcctt ctcgtgatcg gtcagggctt cctggtcgag gtggcgccac
    18601 cctcgttcgt acaggttgag tgcctccgac tcggtgatcg tgacgccggc atcggtctgc
    18661 cacgccagca tgcgcaggcc tgggtactcc ccgaccgtga tcgtcggtga tcctgctcct
    18721 tcagcgtcga ggatcgggac gacgaggtgg agcccaagcg cggctgcaac gttgatgtag
    18781 gcaccgatcg tgaccgaggg acttcctgcc tcgatcctgt gcagggtgac acgcgacacg
    18841 cctgcagcct cggcacaagc gactgccgtg acaccgagcg ccttgcggcg ggcgcgcagg
    18901 cggccgctca ggcgctcgag atcggcctgc tgctcggggc tcgtgtacgg actggacgct
    18961 ggcatgtttc tcattttagg atacttccat caactgtcaa caatgagaaa cgcttgaatg
    19021 ccaggggtgt caggcgtagt cgggaacgga gaccagcctc aacccagggt gtcaggcccg
    19081 tctcggttct gatgggtcag tacgagctct ggcgcggcga gtggccggga ggcccggtga
    19141 ctcgcctcac cgagcgcgag acgcaggcgc tcgccagcga tgatgtggcg tggcacgatc
    19201 cagagcacgc aacactggtt gtctggattc gcgaagggcc ttgactgctc gatgaccgcg
    19261 ggagcggtca tcgatcccta ccgctctatc cgcggcttat ccggtaggca actcacgctg
    19321 attagcgtga aacgacgtcg ggacggggta gcgccacttg ccggactggc gtacagtttt
    19381 ccgtacagga ggtcaccatg aagacgatga gctacaccga gtcccgtgct cgctacgccg
    19441 aggtgctcga cggcgttgcc aacgaccggg aggaagtcat catcacccgg gccgggcacg
    19501 accccgtggt catcgtctcc ctcgaggact acgaatcttt gcgcgagacg gcgtacctga
    19561 tgcgctcgcc ggccaatgct cgtcgcctat tggatgccat ggagcgcctc gaggcgggcc
    19621 gcggcgagcc gcacgacatg atcgacgccg actgacgtgc tcctcgtctg ggacgagaac
    19681 gcctggcagg actacctctg gtggcaggcc caggaccgca gggtcctgaa acggatcaac
    19741 acgctcttgg cagacatcga gcgcaacggc aacgagggca tcggcaagcc ggaggcactc
    19801 aagcacgact tcgccggcta ctggtccaga cgcatcaccg acgagcaccg cctggtatac
    19861 aaactcgtcg gccaagaagt tcgtattgcc gcctgccgct accactacgg gtagcgcgat
    19921 ctgcggca
//
LOCUS       DAOSOC010000027        19020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_2905_length_19020_cov_10.359663, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000027 DAOSOC010000000
VERSION     DAOSOC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 19020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19020
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2905_length_19020_cov_10.359663"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="PL014_03880"
     CDS             complement(<1..104)
                     /locus_tag="PL014_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT56260.1"
                     /translation="MDEQAPHPVTGQLFASPVPPGRGWPGDPATAATPV"
     gene            162..1115
                     /locus_tag="PL014_03885"
     CDS             162..1115
                     /locus_tag="PL014_03885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT56261.1"
                     /translation="MAEVTVVGGGVVGLTSALELARAGHEVRCVRDQPVAETVSAVAG
                     GLWFPYHVEPRDRVVAWGAVALARFADLSGDPASGVVMSEGLLVERGPADRWWTQSVP
                     SWRDASGAELPEGATGGVVATVPLVTMPVFLPWLEMQCRDAGVAFEQAHVSTLDGLEG
                     DVVVVAAGLRSGALVDDDAGMPSRGQVALLENPGLTRWLVDDDHPDGLLYVLPHPGWV
                     VCGGTDVSGSWDTQPDPQVHDAIVRRVRDAVPALATARVIGPRVGLRPVAPEVSLTAS
                     DIGGRVVVTNHGHGGAGVTLSWGCAQEVVRLVGEAAGLSRP"
     gene            complement(1102..2331)
                     /locus_tag="PL014_03890"
     CDS             complement(1102..2331)
                     /locus_tag="PL014_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOT56262.1"
                     /translation="MESEQPASSRRHRTKEQVTALIRSGPPLTRADLGQATGLSRSAV
                     AAVVQELLDEGVIEEDLIPPGGRGSGRGRPSALLVPSAARGHVVAIDFGHNHLAVAVA
                     DAEGGVLAERWAQMDVDGQARASLDAAVEIVVELLDEAGVAVSDIRCAAAGIPAPIDA
                     HSHEIRSMSIMSDWVGVNPQQELARRLGWRVVVANDADMGAQGELRFGAARGIRDLVY
                     VKLGDGVGASLVLQGVPYPGADGLAGEVGHTQVSDQGDWCRCGNRGCLETVASTAFLR
                     TLMADAGLSPRADAYPLRDAAEHPAVARFVEETGRTVGRVLADLCNWLNPRGVILGGE
                     LSTVGAPLADGVRESIRRYAQPASAQAVEVRTAELGRRSELLGAVAVACRELGYEPWA
                     KPLPPIALDAARRVTAG"
     gene            2586..4166
                     /gene="gguA"
                     /locus_tag="PL014_03895"
     CDS             2586..4166
                     /gene="gguA"
                     /locus_tag="PL014_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT56263.1"
                     /translation="MTSPEGSGRGRPDYILEMRDITKEFPGVKALSNVDMAVRRGEIH
                     AICGENGAGKSTLMKVLSGVHPTGSYTGEIHFNGKLAEYSNVRDSERDGIVIIHQELA
                     LIPELSIAENIFLGNERASKSGVIDWFATRQMAAELMERVGLSEDPLTKTKDIGVGKQ
                     QLVEIAKALSKEVKLLILDEPTAALNEGDSQHLLQIMDGLRQRGITCIMISHKLNEIE
                     QISDAITIIRDGKTIETLRVDEGGVDENRIIRGMVGRDLESRFPEHTPKIGEVFFEVK
                     NWRVQHPTRADRLVCKDSSFVVRRGEIVGFAGLMGAGRTELMRSIFGRSYGTVLGGTI
                     TKDGKDLVLHHVADAIHEGIAYVTEDRKTLGVNLLDDIKTTTVSANLERITQGLVVNA
                     SEEYRYAEEYRVNLRTKAPNVDEGVSKLSGGNQQKVVLAKWLFTEPDLLILDEPTRGI
                     DVGAKYEIYGIIQRLADEGKGVIVVSSELPELLGLSDRIYTLFEGQITGVLDKAEANQ
                     ESLMKLMTKARTTASVAS"
     gene            4189..5481
                     /gene="gguB"
                     /locus_tag="PL014_03900"
     CDS             4189..5481
                     /gene="gguB"
                     /locus_tag="PL014_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT56264.1"
                     /translation="MNVIKKIVGGDSRQLGMIFALIALIAFFQWRTGGLTLTPDNVNN
                     IIQGNAYVLVLAIGMVLVIIAGHIDLSVGSIAAFVGIVVALAITDWGLPWPVAILLGI
                     GVGALCGAWQGLWVAYVGIPAFIVTLAGMLIFRGLNQWIGKSNTVPVPEGFQVIGAGS
                     LPEWGPNTGYNNLTLLLGLAGCAAIIVGALRSRAKLTKIGADVEEAWVMWTRLALISA
                     VIMGVMMIIASGRKGFPVSGLILVALVIGYGFLANKMILGRHIYAVGGNRHAAELSGV
                     KSKKVNFFVMANMAVLASLAGMMFVARSNASGPFDGVGWELDAIAAVFIGGAAVTGGV
                     GTVVGSIVGGLVMAVLNNGLQLLGIGADRTQVIKGLVLLIAVAFDVYNKSQGKPSITG
                     LLMRNKTAAAPAAPTAVDAGNPASAAVTDTSTVVKTEG"
     gene            5583..6689
                     /gene="chvE"
                     /locus_tag="PL014_03905"
     CDS             5583..6689
                     /gene="chvE"
                     /locus_tag="PL014_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT56265.1"
                     /translation="MRSLTKAVAVTAALGLALTGCGRTEEATPEASGSGSTAAAGGFP
                     ADSAIGIALPQKTSENWVLAENLFNEGLTAAGFKGDVQFANGGVSEQQNQIQAMITKG
                     AKVIVVGAIDGAQLGTQLKAAKDSGATVIAYDRLLTNTADVDYYIAYDNFKVGQLQGQ
                     ALLDGLAMKKPAGPYNIELFAGSPDDNNAKVFFDGAMEVLKPKIDDGTLKVVSGQTTF
                     EQAVTQGWKAENAQKRMDTLLAGSYTGSTALDGVLSPNDTLARAIITSVKAAGKPIPV
                     VTGQDSEVESVKSIMAGEQFSTINKDTRKLVEETIAMVVALQKGEEPTITDTTSYNNG
                     VKVVPANLLEPVIVTKANAAEAYANDPVLGPLTK"
     gene            complement(6815..7264)
                     /gene="arfB"
                     /locus_tag="PL014_03910"
     CDS             complement(6815..7264)
                     /gene="arfB"
                     /locus_tag="PL014_03910"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOT56266.1"
                     /translation="MMDLVVPPGPGVPQGLTIPAGEIVERFSRSQGPGGQGVNTADSR
                     VEIELDVVTCPAFTDTQRQRVLRALDSRLVGGRLLVVAASEHRQQRRNRVAARERMAD
                     LLRTAMAPPPAPRRATRPTRGSTERRLTAKRHRGETKAKRARPASDA"
     gene            complement(7261..8250)
                     /gene="ligD"
                     /locus_tag="PL014_03915"
     CDS             complement(7261..8250)
                     /gene="ligD"
                     /locus_tag="PL014_03915"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOT56267.1"
                     /translation="MASRKGEFGEPVVLDVEGFDVRVSSPQRVYFAARGETKLDLVNY
                     YLSVGDGIVRALRERPCMLHRFPDGVSGPKVHQKRLPRGAPPWVETVRLHFPRYGLHA
                     DELCVTRLADVAWAVQMSTVEFHPWNSRRADTEKPDEWRIDLDPMPDAPFSRVRRAAQ
                     VSHEVLDELGAVGWPKTSGGSGLHVYVRIAPDHGFGDVRRAALAFAREVERRLPDDVT
                     TTWWRKDRDPGAVFIDYNQNARDHTIAAAYSVRGNATGTVSTPIRWDEVDDVEPRELT
                     IATVPARFAALGDLHAGIDDAVFDLAPLLEWADRDEAQGALPPPEPEDDTGRP"
     gene            8298..10715
                     /gene="pcrA"
                     /locus_tag="PL014_03920"
     CDS             8298..10715
                     /gene="pcrA"
                     /locus_tag="PL014_03920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HOT56268.1"
                     /translation="MSTLFDGLDFGEFDPAKVDAAGVPTWAQAAAAGRGPDGGPDALE
                     RGPVRTDPEELLVGLNPQQREAVLHEGGPLLIVAGAGSGKTRVLTQRIAYLLAARGVQ
                     PGQILAITFTNKAAAEMRERVSALVGPRAKAMWVMTFHSACVRILRREADKVGMRSTF
                     SIYDAADSQRLMSMVLRDMDLDPKRYNPRSFSHQVSNLKNELVDEETFASRTGEASPH
                     QEQVLAEAYSMYQRRLRQANAMDFDDLIMTTVHMFQAFPDVAEHYRRRFRHVMVDEYQ
                     DTNHAQYQLIRELVGSESTRAEHGVEPSELVVVGDADQSIYAFRGATIRNIEEFEVDY
                     PDARTILLEQNYRSTQTILRAANAVIARNEKRREKNLWSQSGDGARIVGYVGDNEHDE
                     ASFVARTIDRLGDEHGIRPRDVAVFYRTNAQSRALEEVFVRVGLPYKVVGGTRFYERR
                     EVKDALAYLRVLSNPTDTVNLRRILNVPKRGIGDRAEACVSALAERERIPFVAALGRP
                     EDAPGIATRSVTAIKGFTTLLESLGHVRDDEDAGVADLLEAVLDRSGYVAELRESRDP
                     QDETRVENLAELVAVAQEFDEARRETGEVIALEDFLEQVSLVADADEIPDTEEAEAAG
                     VVTLMTLHTAKGLEFPVVFLTGMEDGTFPHLRSLADPKELEEERRLAYVGITRARERL
                     HLSRAAVRSAWGAPQYNPPSRFLDEIPAELLQWERELSGAAAVGRRDQRPAVATLAAR
                     PGVRSPGNRPVIALSAGDRVTHDSYGLGTVVRTLGEGDKTQAEVDFGGELGVKRFVLR
                     YAPLEKL"
     gene            complement(10729..11427)
                     /locus_tag="PL014_03925"
     CDS             complement(10729..11427)
                     /locus_tag="PL014_03925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT56269.1"
                     /translation="MLPTAAAAALMVTATGATMAESAVPVAAPLTLDLSGAQSETART
                     EQAADEATVIDLAVRRQDSSMQTAALQGRVEAKDRAARDAKRKAAAKAKARAEAERNG
                     KKWVKAIRTGRHTSDFGPRWGRNHNGIDVAAPVGTPLYAMSKGTVVHAAYSSVAGNKV
                     EIKYWDGTSSTYHHMSRMDVKAGQSVMPGDVVGAVGNTGRSFGAHLHLEIHPGGGDPI
                     NPFPWLKKKGILVG"
     gene            complement(11700..12641)
                     /locus_tag="PL014_03930"
     CDS             complement(11700..12641)
                     /locus_tag="PL014_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT56270.1"
                     /translation="MANSLRPVADPEQDTTPTGLSVMGRARATVDAVAGLSRAALDPA
                     VLAGCAVEAAWMTAHVATWPLGLVGSRASRTGPAYRLEHLPPVQRGLLVSNIEAAGTP
                     ILLVHGIVSNRSIFTLLRRGLTRRGFSNVFAMNYSTILTDVRSAAAGLGAEVEQIVAE
                     TGFERIHIIGHSLGGLIARYYVTRLGGDARVHTLVTLGTPHTGTYAAYAVPTRLMEQM
                     RPGSGLMRELARPVRGCRTRFISYWSDTDLAIVPQRAAALRHQDLSARNVRLHGAGHL
                     TLPMLGEVVHGISTALAHLDADGSTLRSGVTPLVRDE"
     gene            12820..13998
                     /gene="sucC"
                     /locus_tag="PL014_03935"
     CDS             12820..13998
                     /gene="sucC"
                     /locus_tag="PL014_03935"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOT56271.1"
                     /translation="MDLFEYQARDVFEKHGVPVLAGAVATTPEQAREAAERIGAASGG
                     VTVVKAQVKVGGRGKAGGVKVAKTADEAQEHASAILGMDIKGHTVHQVMIAQGARIDE
                     EYYFSILLDRANRTYLAMCSKEGGMDIEQLAVERPDALARVAVDPNVGIDDAKAREIV
                     EQAGFDAETAAKIEPVLKTLWAVYRDEDATLVEVNPLVRTEDGDIVALDGKVSLDANA
                     FFRHPEHEALEDKAETDPLEAAAKEKDLNYVKLDGSVGIIGNGAGLVMSTLDVVAYAG
                     EEFGGQKPANFLDIGGGASAQVMADGLHIILSDPQVKSVFVNVFGGITACDAVANGIV
                     GALDALGDEEDRPLVVRLDGNNVEEGRRILAERNHPRVIIEATMDGAARRAAELASQA
                     "
     gene            14033..14926
                     /gene="sucD"
                     /locus_tag="PL014_03940"
     CDS             14033..14926
                     /gene="sucD"
                     /locus_tag="PL014_03940"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOT56272.1"
                     /translation="MAIFLTADSKVIVQGMTGSEGMKHTQRMLRSGTDVVGGVNPRKA
                     GTTVEFEGGTTVPVFGSVAEAMAATGADVSVIFVPAAFTKAAALEAVDAGIPLAVVIT
                     EGVAVKDTAEFYNYAKDKGTTRIIGPNCPGLISPGAANAGIIPADISGPGRIGLVSKS
                     GTLTYQMMYELRDFGFSTGVGIGGDPIIGTTHIDCLEAFQNDPDTDAIVMIGEIGGDA
                     EERAAAYIKDHVTKPVVGYVAGFTAPEGKTMGHAGAIVSGSSGTAQAKKDALEAAGVK
                     VGKTPSEAAQLMREIMQSLQA"
     gene            14998..16275
                     /locus_tag="PL014_03945"
     CDS             14998..16275
                     /locus_tag="PL014_03945"
                     /inference="COORDINATES: protein motif:HMM:NF040070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HOT56273.1"
                     /translation="MTVVDRLRSAFPGSTDPGDDAEPTDGSTPDTLETAEPAPWWRAA
                     VVGLVTGLASLLVVLAPVVLAWLTDPIDGSTLWQAVGTGAAFWLLSSGAHLSVGPIAL
                     SLVPLLALALLVIVARRGAREALAEVTTEGEHWHGLLPAPLATALGAWWGGYAVAVAA
                     AVGLSIPGAFSVSALSLALPLVLVPLLSAALAVRPLVRDEPDVLGPVLNPDRVPDLVR
                     RGIRPGMAGVRALFAVAAVVVLSAVALSWGEVSTISAEVGAAGVGSVVLALAQLAYLP
                     NIAVWVISFLAGPGFQVVEGGAVTWSGSEGGLLPMVPVLAALPQPGAFPWFVALSVLL
                     VVGVGSFIGRRALREVARLSRLRTKAAAALSACAVTAVVVVALDLVAGGAAGQFRLSA
                     VGAPDLSLFVALFLELSLGALVAVLRDAWTLRR"
     gene            16272..16859
                     /gene="purN"
                     /locus_tag="PL014_03950"
     CDS             16272..16859
                     /gene="purN"
                     /locus_tag="PL014_03950"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOT56274.1"
                     /translation="MTHPLRLVVLVSGSGSNLQALIDAAAEPGAPFRVVAVGADRDGT
                     LGLERAVGNGIPTFVCRVPDYTTRADWDEALARLISDAVPDLVVSAGFMKVLGPTVLA
                     RHRVINTHPALLPSFPGAHGVRDAMAHGVRVTGCTCHWVDAGVDTGAIIDQRAVRVED
                     DDTVETLHERIKVLERDMLVEVVRSLAADRSWQGA"
     gene            complement(16870..17988)
                     /gene="galT"
                     /locus_tag="PL014_03955"
     CDS             complement(16870..17988)
                     /gene="galT"
                     /locus_tag="PL014_03955"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT56275.1"
                     /translation="MSATSTARRTVRNLADGREIIYYDDTPGAPPRTTDDERPLPERA
                     AAGHLRYDALVGDWVAVAGHRMKRTLLPPRDECPLCPTGRGSIATEIPDSDYDVVVIE
                     NRFPSYAPVAVDDAPGEAPFEHRPALGRTEVVCFTSEHDTSFRSLSPRRVRTVIDAWA
                     DRTARLSEIPEVEHVFCFENRGPEIGVTLHHPHGQIYAYPYLPTRSREILAQAQAHHT
                     RTGRLLGADLLRAEQADGSRIVISGTHWTAYVPYAARWPVEVHLAPHRDVPDLVALDD
                     AERDDLATVYLDLLDRLDRCHRTEDDGPVALPYIAAWHQAPVRQGRDVSRLHLQVVSV
                     LRAPGTLKFLAGSESGVGGWVNDARPEAIAARLRSLGG"
     gene            complement(17985..18815)
                     /locus_tag="PL014_03960"
     CDS             complement(17985..18815)
                     /locus_tag="PL014_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOT56276.1"
                     /translation="MLASQRRTAILALVEESGAARVSELVDQLGVSDMTVRRDIERLD
                     AEGLLERVHGGAIALLPRAADEPGFNAKSSLMTAEKQAIAQAAAALVEPGATIGISAG
                     TTTYEFARAIRNMPALTVVTNSVPVAQLLHEGSGNHVVVLTGGVRTPSDALVGPVAVS
                     ALKGLHVDRLFLGAHGIDRNAGLTTPNLVEAETNRALVGASRSVCVLADHTKVGIVGL
                     STFMALDEVDTLITDAGVPSRARGILEESVDHLLLAAVADAHLTSARQAGDVAKGGRQ
                     "
     gene            complement(18818..>19020)
                     /locus_tag="PL014_03965"
     CDS             complement(18818..>19020)
                     /locus_tag="PL014_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Beta-galactosidase C-terminal domain"
                     /protein_id="HOT56277.1"
                     /translation="VEPVVRTSPGIEVVRRRSDSGSWLFVLNHTDDECQVTASGHDLV
                     SSTDVGPTLHLSPRTAAVVREG"
BASE COUNT         2827 a         6724 c         6692 g         2777 t
ORIGIN      
        1 accggggtgg cggccgtcgc cggatcgccc ggccagcccc tgccgggtgg caccgggctg
       61 gcgaagagct gcccggtcac cgggtggggt gcctgctcgt ccatgggctc caccctgaca
      121 ccgagggcgc cgcgacggaa ccgatgcgcg aggatcaccc catggcagag gtgacggtcg
      181 tcggtggagg tgtcgtgggc ctgacctcgg ccctcgagct cgcgcgcgcc gggcacgagg
      241 tgcgctgcgt gcgcgaccag ccggttgccg agacggtgtc agcggtcgcc ggagggctgt
      301 ggttccctta ccacgtcgag ccgcgggacc gcgtggtcgc ctggggtgcc gtggcccttg
      361 ccaggttcgc cgacctgtcg ggcgatccgg catccggcgt ggtgatgagc gaggggctgc
      421 tcgtggagcg agggccggcg gatcggtggt ggacgcagag cgtgccgtcg tggcgcgacg
      481 cgtcgggtgc tgagctgccg gagggcgcca ccgggggagt ggtcgcgacc gttccgctcg
      541 tgacgatgcc cgtgttcctg ccgtggctcg agatgcagtg tcgcgacgcc ggggtcgcgt
      601 tcgagcaggc ccacgtgagc accctcgacg gcctcgaggg tgacgtggtc gtcgtcgccg
      661 ccgggctgcg gtcgggagct ctggtggacg acgacgcggg gatgccgtcg cgtggtcagg
      721 tcgccctgtt ggagaacccc gggctgacgc gctggctcgt cgatgacgac catccggacg
      781 ggctgctcta cgtgttgccg caccccgggt gggtcgtgtg cgggggaacg gacgtcagcg
      841 gttcgtggga cacccaaccc gacccgcagg ttcatgacgc gatcgtgcga cgggttcgtg
      901 acgccgtccc cgcgctcgcg acggcccggg tgatcggacc tcgcgtgggg ttgcgcccgg
      961 tcgcgcccga ggtgtcgctc acggcatccg acatcggggg gcgggtggtg gtgaccaacc
     1021 acggtcacgg cggggccggg gtcaccctgt cgtgggggtg tgcccaggag gtcgtccggc
     1081 tcgtcggcga ggcggcgggt ctcagccggc cgtgacccgg cgcgcggcgt ccagggcgat
     1141 gggcggcagc ggcttcgccc acggttcgta gccgagttcg cggcaggcca cggcaaccgc
     1201 tcccagcagc tcggagcggc gacccagctc ggccgtgcgg acctcgacgg cctgggcact
     1261 cgctggctgc gcgtagcggc ggatcgactc gcgcacgccg tcggccaggg gtgcgcccac
     1321 ggtgctcagc tcacccccga ggatcacgcc gcgcgggttg agccagttgc acaggtcggc
     1381 gagcacccgg cccacggtgc ggccggtctc ctcgacgaag cgcgcgacgg cggggtgctc
     1441 ggcggcatcg cgcagggggt acgcgtcggc gcgggggctc aggcctgcgt cggccatgag
     1501 ggtgcgcagg aaggccgtcg acgcgacggt ctcgaggcag ccacggttgc cgcagcggca
     1561 ccagtcgccc tggtccgaga cctgggtgtg accgacctcg ccggccaggc cgtcggcccc
     1621 cgggtagggc acaccctgga ggacgaggct ggccccgacc ccgtcgccga gcttgacgta
     1681 gacgaggtcg cggatgccgc gcgcggcgcc gaaacgcagc tcgccctgag cgcccatgtc
     1741 ggcgtcgttg gcgacgacga cccgccagcc gaggcggcgc gcgagttcct gctgggggtt
     1801 gaccccgacc cagtcggaca tgatcgacat cgagcggatc tcgtggctgt gggcgtcgat
     1861 cggggccggg atgccggccg ccgcgcaccg gatgtccgac acggcgacac cagcttcgtc
     1921 gagcagctca acgacgatct cgacggccgc gtcgagcgag gcccgggcct ggccgtcgac
     1981 gtccatctgg gcccagcgct cggcaaggac acctccctcg gcatccgcga cggccaccgc
     2041 caggtggttg tggccgaagt cgatggcgac cacgtgccct cgggccgcgc tcggcacgag
     2101 cagcgccgac gggcgtccgc gcccggagcc gcgtccaccc ggagggatga ggtcctcctc
     2161 gatgacgcct tcgtcgagaa gctcctggac gacggctgcg acagcgctgc gcgacaggcc
     2221 ggtcgcctgg ccgaggtcgg ccctggtgag gggcgggccc gagcggatca gcgccgtcac
     2281 ctgttccttg gtgcggtgcc gacggctcga cgcgggctgc tcggactcca tgtcaggact
     2341 ctagggacaa aaataggctc ttgacaagag ttattcccac cgatattgtc cggtaaaact
     2401 tcattagtcg tagaacccgg agtcagcgcc accgcgtccc agctccgggg caaggtgacg
     2461 atttgagaac gtgcgtggac acttcgctcg agtctctaca ggccgtcagg ccgctctggc
     2521 acactcggca accgacgctg gcgtcgtgct ggatcacccc cactcagctg tgcaaaggag
     2581 cacgcatgac atcaccagag ggatcgggcc gaggtcggcc cgactacatc ctcgagatgc
     2641 gggacatcac caaggagttc cccggggtca aggccctgtc caatgtcgac atggcggtga
     2701 ggcgtggtga gatccacgcg atctgcggtg agaacggcgc ggggaagtcc accctcatga
     2761 aggtcctgtc cggggtgcac cccacggggt cctacacggg cgagatccac ttcaacggca
     2821 agctggccga gtactccaac gtccgcgaca gcgagcgtga cgggatcgtc atcatccacc
     2881 aggagctcgc gctcatcccc gagctgtcga tcgccgagaa catcttcctc ggcaacgagc
     2941 gcgcctccaa gagcggtgtc atcgactggt tcgcgacccg ccagatggct gccgagctca
     3001 tggagcgggt ggggctgtcc gaggatccgc tcaccaagac caaggacatc ggcgtcggca
     3061 agcagcagct cgtcgagatc gccaaggcac tgtccaagga ggtcaagctc ctcatcctcg
     3121 acgagcccac cgccgccctg aacgaagggg actcgcagca cctgctgcag atcatggacg
     3181 ggctgcgtca gcgcggcatc acctgcatca tgatcagcca caagctcaac gagatcgagc
     3241 agatctccga cgccatcacg atcatccgcg acggcaagac gatcgagacc cttcgcgtcg
     3301 acgagggtgg tgtcgacgag aaccggatca tccgcggcat ggtcggccgc gacctcgagt
     3361 cgcgcttccc cgagcacacc ccgaagatcg gtgaggtctt cttcgaggtc aagaactggc
     3421 gcgtccagca cccgacccgg gccgaccgac tcgtctgcaa ggacagctcc ttcgtcgtcc
     3481 gccgcggtga gatcgtcggg ttcgccggcc tcatgggcgc tggccgcacc gagctgatgc
     3541 gcagcatctt cggtcgctcg tacggcaccg tgctgggcgg aacgatcacc aaggacggca
     3601 aggacctcgt cctgcaccac gtcgccgatg ccatccacga gggcatcgcc tacgtcaccg
     3661 aggaccgaaa gaccctcggt gtgaacctgc tcgacgacat caagacgacg acggtctcgg
     3721 ccaacctcga gcgcatcacc cagggactgg tggtcaacgc gagcgaggag taccgctacg
     3781 ccgaggagta ccgggtcaac ctgcgcacca aggctcccaa cgtcgacgag ggtgtctcga
     3841 agctgtccgg tggcaaccag cagaaggtcg tgctcgccaa gtggctgttc accgagcctg
     3901 acctgctcat cctcgacgag ccgacccgcg gcatcgacgt cggggcgaag tacgagatct
     3961 acgggatcat ccagcggctg gccgacgagg gcaagggcgt catcgtcgtg tcctccgagc
     4021 tgcccgagct gctcggcctc tcggaccgga tctacaccct cttcgagggg cagatcaccg
     4081 gtgtgctcga caaggcggaa gccaaccagg aatccctcat gaagctgatg acgaaggccc
     4141 ggaccaccgc gtccgtggcc tcctgacgac agacaggacc cgcgagacat gaatgtcatc
     4201 aagaagatcg ttggcggaga ctcccgccag ctgggcatga tctttgccct gatcgccctc
     4261 atcgcgttct tccagtggcg caccggtggc ctcaccctca ccccggacaa cgtcaacaac
     4321 atcatccagg gcaacgcgta cgtgctcgtc ctggccatcg gcatggtgct cgtcatcatt
     4381 gccggccaca tcgacctgtc ggtcgggtcg atcgccgcgt tcgtcggcat cgtcgtcgcc
     4441 ctggcgatca ccgactgggg cctgccctgg cccgtggcca tcctcctcgg catcggcgtc
     4501 ggtgccctgt gtggcgcctg gcagggcctc tgggtcgcct acgtcggcat ccccgcgttc
     4561 atcgtgaccc tggccggcat gctcatcttc cgcggcctca accagtggat cggcaagtcc
     4621 aacaccgtcc ccgtccccga gggcttccag gtcatcggtg ccggctcgct gcccgagtgg
     4681 ggcccgaaca ccggctacaa caacctcacc ctgcttctcg gccttgccgg gtgcgccgcg
     4741 atcatcgtcg gcgccctgcg cagccgcgcc aagctcacca agatcggcgc cgacgtcgaa
     4801 gaggcgtggg tcatgtggac gcggctggca ctcatctccg cggtgatcat gggcgtcatg
     4861 atgatcatcg cctccgggcg caagggcttc ccggtctccg ggctgatcct cgtcgcgctc
     4921 gtcatcggct acggcttcct ggccaacaag atgatcctcg gccggcacat ctacgccgtc
     4981 ggtggcaacc ggcacgcggc tgagctctcc ggtgtgaagt cgaagaaggt caacttcttc
     5041 gtcatggcca acatggcggt gctcgcctcg ctggccggca tgatgttcgt cgcccgctcc
     5101 aacgcctccg gtccgttcga cggcgtcggc tgggagctcg acgcgatcgc ggccgtcttc
     5161 atcggtggcg ctgccgtcac cggcggtgtc ggcaccgtcg tcgggtccat cgtcggtggc
     5221 ctcgtcatgg ccgtgctcaa caacggtctc cagctgctcg gcatcggcgc tgaccgtacc
     5281 caggtcatca agggtctggt gctgctcatc gccgtcgcgt tcgacgtcta caacaagtcg
     5341 cagggcaagc cctcgatcac cgggctgctc atgcggaaca agaccgcagc cgcccccgcc
     5401 gcacccaccg ccgtggatgc cggtaacccc gcgtccgctg cggtgaccga cacctccacg
     5461 gtcgtcaaga ccgaaggctg atgtaaccca cctcggtcgc cgcgacccgg catccgaccc
     5521 gcgcagtacc tgacaccaca tcacccgcgt tcacgcgaac gcaccccaca gaaagaagaa
     5581 ccatgcgatc actcaccaag gccgtcgccg tcaccgcggc gctgggtctg gctctcaccg
     5641 gctgcggccg caccgaggag gccacccccg aggcatcggg ttccggctcg accgcggcag
     5701 ccggcggttt ccccgctgac tccgccatcg gcatcgccct tccgcagaag acctcggaga
     5761 actgggtcct cgccgagaac ctgttcaacg agggcctcac cgcggccggc ttcaagggcg
     5821 acgtgcagtt cgccaacggc ggcgtctccg agcagcagaa ccagatccag gccatgatca
     5881 ccaagggtgc gaaggtcatc gtcgtcggtg ccatcgacgg cgcgcagctc ggcacccagc
     5941 tcaaggcggc caaggactcc ggcgccaccg tcatcgccta cgaccgtctc ctgacgaaca
     6001 cggccgacgt cgactactac atcgcctacg acaacttcaa ggtcggccag ctgcagggtc
     6061 aggccctgct cgacggtctc gccatgaaga agccggccgg cccgtacaac atcgagctgt
     6121 tcgccggttc gcctgacgac aacaacgcga aggtgttctt cgacggcgcc atggaggtgc
     6181 tcaagccgaa gatcgacgac ggcaccctga aggtcgtgtc cggccagacg accttcgagc
     6241 aggccgtcac ccagggctgg aaggccgaga acgcgcagaa gcgcatggac accctcctcg
     6301 ccggctccta caccgggtcc accgccctgg acggcgtcct gtcgcccaac gacaccctgg
     6361 cccgcgcgat catcacctcg gtcaaggccg ccggcaagcc gatcccggtc gtcaccggtc
     6421 aggactccga ggtcgagtcg gtcaagtcga tcatggcggg cgagcagttc tccacgatca
     6481 acaaggacac ccgcaagctc gtcgaggaga cgatcgcgat ggtcgtcgcc ctccagaagg
     6541 gtgaggagcc cacgatcacc gacaccacgt cctacaacaa cggcgtcaag gtcgtcccgg
     6601 ccaacctcct cgagccggtc atcgtcacca aggccaacgc cgccgaggcc tacgccaacg
     6661 acccggtgct cggcccgctg accaagtgag ccacggcctc acggccacct agcaccactc
     6721 gatcggcccg tctgctccgg caggcgggcc gatcgccgtc cggcgacgcg gcatccactg
     6781 ctcgttcccg tgcccggatg ccgggcggtg cgggtcaggc gtcggatgcc ggtcgcgcgc
     6841 gtttcgcctt cgtctcgccc cggtgccgct tggcggtgag gcggcgttcc gtcgagccgc
     6901 gggtggggcg ggtggcgcgg cgaggagccg ggggaggggc catcgcggtg cgcaggaggt
     6961 cagccatccg ctcgcgggcg gccacccggt tgcgccgctg ctggcggtgc tcggaggcgg
     7021 cgacgacgag caggcggccc ccgaccagtc gcgagtccag ggcccgcagt acccgttggc
     7081 gctgggtgtc ggtgaacgcc ggacaggtca cgacgtcgag ctcgatctcg acgcggctgt
     7141 cagcggtgtt caccccctgc ccgccgggcc cctgcgagcg ggagaaccgc tcgacgatct
     7201 caccggcggg gatcgtcaag ccctggggaa cgcccgggcc cggcgggacg acgaggtcca
     7261 tcagggccgg ccggtgtcgt cctcgggctc gggaggcggc aatgccccct gcgcctcgtc
     7321 acgatcggcc cactccagga gcggggcgag gtcgaacacc gcgtcgtcga tgccggcgtg
     7381 caggtcgccg agggctgcga accgggccgg gacggtggcg atggtgagct ctcgtggctc
     7441 gacgtcgtcg acctcgtccc agcggatcgg cgtcgacacc gtgccggtgg cgttgccgcg
     7501 aaccgagtac gcggcggcga tggtgtggtc gcgggcgttc tggttgtagt cgatgaacac
     7561 ggcgccgggg tcgcggtcct tgcgccacca ggtggtcgtc acgtcgtcgg gcaagcggcg
     7621 ttcgacctcg cgggcgaagg cgagggctgc tctgcgcacg tcgccgaacc cgtggtcggg
     7681 agcgatccgc acgtacacgt gcaggcccga cccgccactg gtcttgggcc accccacggc
     7741 gccgagctcg tcgagcacct cgtgcgagac ctgcgccgcg cgtcgcacgc gcgagaacgg
     7801 agcatccggc atcgggtcga ggtcgatgcg ccactcgtcc ggcttctcgg tgtcggcccg
     7861 ccggctgttc cacgggtgga actccacggt gctcatctgc acggcccagg ccacgtcggc
     7921 caggcgggtc acgcacagct cgtcggcgtg caggccgtag cgcgggaagt gcaaccgcac
     7981 cgtctcgacc cacggcgggg cgccccgggg caggcgcttc tggtggacct tcggcccact
     8041 caccccgtcc gggaagcggt gcaacatgca gggtcgctcg cgcagggccc gcacgatgcc
     8101 gtcgccgacg gacaggtagt agttcacgag gtcgagcttg gtctcgccac gggccgcgaa
     8161 gtagacccgc tgcggactgg acacccgcac gtcgaagccc tcgacgtcga ggacgacggg
     8221 ttccccgaac tcacccttgc gcgaggccat gaccgacacc gtatgccgtc agcgggggga
     8281 cgtagggttg acccgacatg agcactctct tcgacggcct cgacttcggt gagttcgacc
     8341 ctgccaaggt cgacgccgcc ggggttccga cgtgggccca ggccgccgct gccgggcgtg
     8401 ggcctgacgg cggccccgac gccctggagc gggggccggt gcgcaccgac cccgaggagc
     8461 tgctcgtcgg cctgaacccg cagcagcgcg aagccgtgct ccacgagggt gggccgctgc
     8521 tgatcgtcgc cggtgccggg tcgggcaaga cccgggtgct gacccagcgc atcgcgtacc
     8581 tcctggctgc ccgcggggtg caaccgggtc agatcctcgc gatcacgttc accaacaagg
     8641 ccgccgccga gatgcgcgag cgggtgtccg ctctcgtcgg gccacgggcc aaggcgatgt
     8701 gggtgatgac cttccacagt gcctgcgtgc gcatcctgcg ccgcgaggcc gacaaggtgg
     8761 gcatgcgctc gaccttctcg atctacgacg ccgcggactc gcagcgcctg atgtcgatgg
     8821 tcctgcgcga catggacctc gaccccaagc gctacaaccc gcgctcgttc agccaccagg
     8881 tgtccaacct caagaacgaa ctcgtcgacg aggagacctt cgcgagtcgc acgggcgaag
     8941 ccagcccgca ccaggagcag gtgctcgccg aggcctactc gatgtaccag cgccgcctgc
     9001 gtcaggccaa cgccatggac ttcgacgacc tcatcatgac cacggtccac atgttccagg
     9061 ccttccccga cgtggccgag cactatcgac ggcgcttccg gcacgtcatg gtcgacgagt
     9121 accaggacac caaccacgcg cagtaccagc tgatccgtga gctcgtcggc tccgagagca
     9181 cccgcgccga gcacggcgtc gaacccagcg agctcgtcgt cgtcggtgac gccgaccagt
     9241 cgatctacgc cttccggggg gcgacgatcc gcaacatcga ggagttcgag gtcgactacc
     9301 ccgacgctcg caccatcctc ctggagcaga actaccgctc cacccagacc atcctgcggg
     9361 ctgccaacgc ggtcatcgcc cgcaacgaga agcggcgcga gaagaacctg tggagccaga
     9421 gcggcgacgg cgcgcgcatc gtcggctacg tcggcgacaa cgaacacgac gaggcgtcgt
     9481 tcgtggcgcg caccatcgac cgactgggtg acgagcacgg catccggcca cgcgacgtcg
     9541 cggtcttcta ccgcaccaac gcccagtccc gggccctgga agaggtcttc gtgcgcgtcg
     9601 gcctgcccta caaggtggtc ggtggcacga ggttctacga gcgccgcgag gtcaaggacg
     9661 cgttggccta cctgcgggtg ctgtccaacc cgaccgacac ggtgaacctg cgccgcatcc
     9721 tcaacgtgcc caagcgcggc atcggcgacc gggccgaggc gtgtgtcagc gccctcgccg
     9781 agcgcgagcg gatcccgttc gtggcggcgc ttggccgccc cgaggacgcc cccggcatcg
     9841 ccacccggtc ggtgaccgcc atcaaggggt tcaccactct cctggagagc ctcgggcacg
     9901 tgcgtgatga cgaggatgcc ggcgtggccg acctgctcga ggccgtcctc gaccgctcgg
     9961 ggtacgtcgc cgagctgcgc gagagccgcg acccgcagga cgagacgcgc gtcgagaacc
    10021 tcgccgagct cgtcgccgtc gcccaggagt tcgacgaggc gcgccgcgag accggcgagg
    10081 tcatcgccct cgaggacttc ctcgagcagg tctcactcgt ggccgacgcc gacgagatcc
    10141 ccgacaccga ggaggccgag gcggccgggg tggtgaccct gatgaccctg cacacggcca
    10201 aggggctgga gttccccgtc gtcttcctca ccggcatgga ggacggcacc ttcccgcacc
    10261 tgcgcagcct ggccgacccc aaggagctcg aggaggagcg ccgactggcc tacgtcggga
    10321 tcacccgggc ccgtgagcgg ctgcacctgt cgcgggcagc agtgcgttcg gcgtggggag
    10381 cgccgcagta caacccgccg tcacgcttcc tcgacgagat ccccgctgaa ctgctccagt
    10441 gggagcgcga gctctccggg gcggctgccg tggggcgtcg ggaccagcgt ccggccgtcg
    10501 cgaccctcgc cgctcgtccg ggcgtgcgat cgccgggcaa ccggccggtc atcgcgttgt
    10561 cggcggggga ccgggtgacc catgacagct atggcctggg caccgtcgtg cgcaccctcg
    10621 gagagggtga caagacgcag gccgaggtgg acttcggcgg agagctcggg gtcaagcgct
    10681 tcgtgctgcg ctacgccccg ctcgagaagc tctaggagat catcctcgtc agccgacgag
    10741 gatgcccttc ttcttcagcc aggggaaggg gttgatcggg tcgccgccac cggggtggat
    10801 ctccaggtgc aggtgcgccc cgaacgaacg cccggtgttg cccacggcgc ccacgacgtc
    10861 gccgggcatg acgctctggc ccgccttgac gtccatccgg ctcatgtggt ggtacgtcga
    10921 cgacgtgccg tcccagtact tgatctcgac cttgttgccg gcgacggaac tgtaggcggc
    10981 gtggacgacc gtgcccttgg acatcgcgta cagcggggtg ccgaccggcg ccgccacgtc
    11041 gatgccgttg tggttgcggc cccagcgggg gccgaagtcg gaggtgtggc ggccggtgcg
    11101 gatcgccttg acccacttct tgccgttgcg ctctgcctcg gcccgggcct tcgccttggc
    11161 ggccgccttg cgcttggcgt cgcgggcagc gcgatccttg gcctcgacac gaccttggag
    11221 ggctgccgtc tgcatcgagg agtcctgtcg gcgcaccgcg aggtcgatga ccgttgcctc
    11281 gtcagcagcc tgctccgtgc gcgccgtctc cgactgcgct ccagagaggt cgagcgtcag
    11341 cggggccgcg accggaaccg ccgactcggc catcgtggcg ccggttgccg tcaccatgag
    11401 cgcggcagcc gcggccgtcg ggagcacgaa gctcgagctc agggctcgag gcaggcgcgc
    11461 actgcgagcc ggacggtgcc gaccggcata gtgacgccct tgataggacg aagaactcaa
    11521 gagggtcctc aagggtcagg ggaaactggg cttgcctcac cctaccgtga ccattccgtg
    11581 atacaaattc cgcgtccaca ccccggcccc ttcagttacg cgcgcgcggt cgccgcacac
    11641 ccctgacgtg ggcttttggc ccgcccgtga tccccgtcac gaccgtgggc gccgggccgt
    11701 cactcgtcac ggacgagggg ggtgacgccc gagcgcaacg tgctgccgtc ggcatccagg
    11761 tgcgccaggg cggtcgagat gccgtgcacg acctcgccga gcatcggaag cgtcaggtgc
    11821 cccgcgccgt ggaggcgcac gttgcgggcg ctcaggtcct ggtggcgcag ggccgcggcc
    11881 cgctgcggca cgatcgcgag gtcggtgtcc gaccagtagg agatgaacct ggtgcggcag
    11941 ccccgcacgg ggcgggcgag ctcgcgcatg agcccgctgc cgggccgcat ctgctccatc
    12001 agccgggtcg gcacggcgta ggcggcatac gtgccggtgt ggggcgtgcc cagggtgacc
    12061 agggtgtgca cccgggcgtc cccgccgagc cgggtgacgt agtagcgggc gatgagcccg
    12121 ccgaggctgt gcccgatgat gtggatgcgc tcgaagccgg tctcggcgac gatctgctcg
    12181 acctcggccc cgaggccggc tgccgcggac cgcacgtcgg tgaggatcgt cgagtagttc
    12241 atcgcgaaga cgttcgagaa cccccgacgg gtcagcccgc gccgcagcag cgtgaagatc
    12301 gaccggttgc tgacgatgcc gtggacgagc aggatcgggg tgccggcggc ctcgatgttg
    12361 gagacgagca ggccgcgttg cacggggggc aggtgctcga gccggtacgc cggcccggtg
    12421 cgcgacgcgc gactgccgac caggccgagc ggccacgtcg cgacgtgggc cgtcatccac
    12481 gcagcctcca cggcgcagcc ggcgagcacc gcggggtcca gcgccgcccg cgagagccct
    12541 gccacggcat ccacggtggc ccgcgcacga cccatcaccg acagccccgt gggggtggtg
    12601 tcctgctcgg ggtcggccac cggccgcagg gagttcgcca tggctcctcc actgtagggc
    12661 gcggatgccg cccgcaccgg tcgtcgcgga ccctgacctt ccagcgtgag aagcgtccca
    12721 cccagcgccc ggagcagacc tcggctgccc atgagggtgc tgaccccgtt aggctcccca
    12781 ccggaccacc tactgccaac agcaaggacg gatcgccccg tggatctctt cgagtaccag
    12841 gcccgtgacg tgttcgagaa gcacggtgtg cccgtgctcg ccggggccgt cgccacgaca
    12901 ccggaacagg cccgtgaggc ggccgagcgc atcggcgcgg ccagtggcgg ggtgaccgtg
    12961 gtcaaggccc aggtcaaggt cgggggccgc ggcaaggcag gcggcgtcaa ggtcgcgaag
    13021 accgccgacg aggcgcagga gcacgccagt gcgatcctcg gcatggacat caagggccac
    13081 accgtgcacc aggtgatgat cgcccagggc gcccgcatcg acgaggagta ctacttctcg
    13141 atcctgctcg accgcgcgaa ccgcacctac ctggccatgt gctccaagga gggcggcatg
    13201 gacatcgagc agctcgccgt cgagcgcccg gacgccctgg cccgcgtcgc cgtcgacccc
    13261 aacgtcggca tcgacgacgc caaggcccgc gagatcgtcg agcaggccgg cttcgacgcc
    13321 gagaccgccg cgaagatcga gcccgtgctc aagaccctct gggcggtcta ccgcgacgag
    13381 gacgccaccc tcgtcgaggt caaccccctc gtgcgcaccg aggacggcga catcgtggcg
    13441 ctggacggca aggtctcgct cgacgcgaac gccttcttcc gccacccgga gcacgaggcg
    13501 ctcgaggaca aggccgagac cgaccctttg gaggccgctg ccaaggagaa ggacctcaac
    13561 tacgtcaagc tcgacggctc ggtcggcatc atcggcaacg gtgcgggcct ggtcatgagc
    13621 accctcgacg tcgtggccta cgccggcgag gagttcggtg gccagaagcc cgccaacttc
    13681 ctcgacatcg gtggcggcgc gagcgcccag gtcatggccg acgggctgca catcatcctg
    13741 tccgacccgc aggtcaagag cgtcttcgtc aacgtcttcg gcggcatcac cgcctgtgac
    13801 gccgtcgcca acggcatcgt cggggcgctc gacgccctcg gcgacgagga ggaccgcccg
    13861 ctggtcgtcc gcctcgacgg caacaacgtc gaggagggtc ggcgcatcct cgcggagcgc
    13921 aaccaccccc gagtcatcat cgaggccacg atggacggcg ccgcgcgccg tgccgccgag
    13981 ctggcttccc aggcctgacc cacccgagcg cgaagagaga cgtagacaac ccatggccat
    14041 cttcctcacc gcagactcca aggtcatcgt ccagggcatg accggctccg agggcatgaa
    14101 gcacacgcag cggatgctgc gctccggcac cgacgtcgtc ggcggcgtca acccccgcaa
    14161 ggccggcacc acggtcgagt tcgagggcgg aacgaccgtc cccgtgttcg gcagcgtcgc
    14221 cgaggcgatg gcggccaccg gggctgacgt cagcgtcatc ttcgtgcccg ccgccttcac
    14281 gaaggccgcc gccctcgagg ccgtcgacgc cggcatcccg ctcgccgtcg tcatcaccga
    14341 gggcgtcgcc gtcaaggaca ccgcggagtt ctacaactac gccaaggaca agggcacgac
    14401 ccgcatcatc gggccgaact gcccgggtct gatcagcccc ggtgccgcca acgcgggcat
    14461 catcccggcc gacatctccg gccccggccg catcgggctg gtgtcgaagt cgggcaccct
    14521 gacctaccag atgatgtacg agctgcggga cttcgggttc tccacgggcg tcggcatcgg
    14581 cggtgacccg atcatcggca ccacccacat cgactgcctc gaggcgttcc agaacgaccc
    14641 cgacaccgac gccatcgtca tgatcggcga gatcggtggc gatgccgagg agcgggccgc
    14701 ggcctacatc aaggaccacg tcaccaagcc ggtcgtcggc tacgtcgcgg gcttcaccgc
    14761 ccccgagggc aagacgatgg gccacgcagg cgcgatcgtg tcgggctcgt cgggcacggc
    14821 ccaggccaag aaggacgccc tcgaggcggc cggggtcaag gtcggcaaga cgccgtccga
    14881 ggcggcccag ctgatgcgag agatcatgca gtccctccag gcctgattca cacgagttcg
    14941 agggccgctc ggcgttgcac tgcaactgtc gggcggccct cgcgcacact ggtgccgatg
    15001 actgtcgtcg accgcctccg ctccgccttc cccggctcca ccgaccccgg ggacgatgcc
    15061 gagcccaccg acggctcgac cccggacacc ctcgagaccg ctgagcccgc tccgtggtgg
    15121 cgcgcagccg tcgtcgggct ggtgacgggg ctggcctccc tgctcgtcgt gctcgcaccg
    15181 gtggtgctgg cctggctcac cgacccgatc gacggcagca ccctgtggca ggccgtcggc
    15241 accggcgcag cgttctggct gctcagttcg ggggcccacc tctcggtggg gccgatcgca
    15301 ctctccctcg tgccgctgct ggcactggcc ctgctcgtga tcgtggcccg acgcggcgcc
    15361 cgcgaggcgt tggcggaggt caccaccgag ggcgagcact ggcacggact gctccccgcc
    15421 ccgctcgcaa ccgcgctggg tgcgtggtgg ggcgggtacg ccgtcgccgt cgccgcagcg
    15481 gtcgggctca gcatcccggg cgcgttctcg gtctccgcgc tcagcctcgc cctcccgctg
    15541 gtcctcgtac cgctgctgtc cgccgccctg gccgtgcgcc ccctcgtgcg cgacgaaccc
    15601 gacgtcctcg gaccggtgct gaaccccgat cgcgtccctg acctggtccg tcgcggcatc
    15661 cgcccgggga tggcgggtgt gcgggcgctg ttcgccgtgg cagccgtcgt cgtcctgagc
    15721 gccgtggccc tgtcgtgggg ggaggtgtcc accatctccg cggaggtcgg tgctgcgggc
    15781 gtcggcagcg tggtgctggc cctggcccag ctcgcctacc tgcccaacat cgcggtgtgg
    15841 gtcatctcct tcctcgcagg cccggggttc caggtcgtcg aggggggcgc cgtcacctgg
    15901 tcgggctccg agggtggcct gctgccgatg gtcccggtgc tcgcagccct gccgcagccc
    15961 ggagccttcc cgtggttcgt ggcgctcagc gtgctcctcg tcgtgggcgt ggggtcgttc
    16021 atcggccggc gtgcgctgcg tgaggtcgcc cggctgtcac ggctgcgcac caaggccgcg
    16081 gccgcgctgt cggcgtgcgc ggtgacggca gtggtcgtcg tcgccctcga cctcgtcgcc
    16141 ggcggcgccg cggggcagtt ccggctctcg gccgtgggcg caccggacct gagcctgttc
    16201 gttgccctct tcctcgagct gtccctcggc gcactggtcg ccgtgctgcg cgacgcctgg
    16261 accctgcggc gatgacccac cccctgcgcc tggtcgtcct cgtctccggc tcggggagca
    16321 acctgcaggc cctcatcgac gccgccgcag agcccggcgc cccgttccgg gtggtcgccg
    16381 tcggggccga ccgcgacggc accctgggcc tcgagcgcgc ggtgggcaac ggcatcccga
    16441 cgttcgtctg ccgcgttccc gactacacga cccgcgccga ctgggacgag gcgctggcgc
    16501 gcctgatctc ggatgccgtc cccgacctcg tcgtgtcggc gggcttcatg aaggtgctgg
    16561 gccccacggt gctggcccgc caccgggtga tcaacacgca cccggcgctg ctgccgagct
    16621 tccccggcgc ccacggggtg cgtgacgcga tggcgcacgg cgtgcgggtc acggggtgca
    16681 cgtgccactg ggtcgacgca ggcgtcgaca cgggggcgat catcgaccag cgggcggtgc
    16741 gcgtcgagga cgacgacacc gtcgagaccc tgcacgaacg gatcaaggtg ctcgaacggg
    16801 acatgctcgt cgaggtcgtg cgctcgctcg ccgcagaccg ctcgtggcag ggcgcctgag
    16861 tcggtgggct cagccgccga gggagcgcag tcgcgcggcg atcgcctcgg gtcgggcgtc
    16921 gttgacccag ccgccgacgc cgctctcgga cccggcgagg aacttcagcg ttcccggcgc
    16981 gcgcagcacc gagaccacct ggaggtgcag gcgcgagacg tcgcggccct ggcgcaccgg
    17041 ggcctggtgc caggcggcga tgtagggcag ggcgacgggg ccgtcgtcct cggtgcggtg
    17101 gcagcggtcg aggcgatcca gcaggtcgag gtagaccgtg gcgaggtcgt cgcgctcggc
    17161 gtcgtcgagg gcgaccaggt cgggcacgtc gcggtgcggg gcgaggtgga cctcgaccgg
    17221 ccagcgggcc gcgtagggca cgtaggcggt ccagtgggtg ccactgatga cgatgcgcga
    17281 gccgtcggcc tgctccgcgc gcagcaggtc ggccccgagc aggcggccgg tgcgggtgtg
    17341 gtgcgcctgc gcctgcgcca ggatctcgcg gctgcgggtc ggcaggtagg ggtaggcgta
    17401 gatctgaccg tgcgggtggt gcagggtgac gccgatctcc gggccacggt tctcgaagca
    17461 gaacacgtgc tcgacctcgg ggatctccga gagccgggcc gtgcgatcgg cccacgcgtc
    17521 gatgaccgtg cgcacgcggc gcggtgacaa cgaccggaag ctcgtgtcgt gctcgctcgt
    17581 gaagcacacc acctcggtgc ggcccagcgc gggccggtgc tcgaaggggg cctcaccagg
    17641 ggcgtcgtcc accgcgacgg gggcgtagga cgggaagcgg ttctcgatga cgacgacgtc
    17701 gtagtcggag tcggggatct cggtcgcgat gctgccgcgg ccggtggggc acagcgggca
    17761 ctcgtcgcgc ggtggcagca gcgtgcgttt catccggtgg ccggcgaccg ccacccagtc
    17821 gccgacgagc gcgtcgtagc gcaggtgccc ggcggcggcc cgctcgggga gggggcgctc
    17881 gtcgtcggtg gtgcgcggcg gggcaccggg ggtgtcgtcg tagtagatga tctcgcggcc
    17941 gtcggccagg ttccgcacgg tgcgccgggc cgttgaggtg gcgctcactg ccggccaccc
    18001 ttcgcgacgt cgccggcctg gcgggcgctg gtcaggtggg catcggccac ggcggcgagc
    18061 aggaggtggt cgacgctctc ctcgaggatg ccgcgcgctc gcgacggcac tcccgcgtcg
    18121 gtgatgaggg tgtcgacctc gtccagggcc atgaacgtcg acaggccgac gatccccacc
    18181 ttggtgtggt ccgccagcac gcacaccgag cggctggcac ccacgagggc gcggttggtc
    18241 tcggcctcga cgaggttggg ggtggtgagc ccggcgttgc ggtcgatgcc gtgggcaccg
    18301 aggaagaggc ggtcgacgtg caggcccttc agcgcggaca cggcgaccgg cccgacgagg
    18361 gcgtcggacg gggtgcgcac cccacccgtg agcacgacca cgtggttgcc gctgccctcg
    18421 tggagcagct gggcgaccgg cacggagttc gtcaccaccg tgagggcggg catgttgcgg
    18481 atggcccggg cgaactcgta cgtcgtcgtc ccggcggaga tcccgatggt ggccccgggc
    18541 tcgacgaggg cggccgcggc ctgggcgatc gcctgcttct ccgcggtcat cagcgaggac
    18601 ttggcgttga agccgggctc gtcggcggcc cgcggcagca gggcgatggc gccaccgtgc
    18661 acccgctcga gcagtccctc ggcgtcgagg cgctcgatgt cgcgccgcac ggtcatgtcc
    18721 gacacgccga gctggtcgac gagttcggac acccgcgcgg cacccgactc ctcgacgagc
    18781 gcgaggatgg cggtgcggcg ctggctggcg agcacgctca cccctcccgc accacggctg
    18841 cggtgcgcgg cgacaggtgg agcgtcggcc cgacgtccgt gctcgacacg aggtcgtggc
    18901 cggatgccgt cacctggcac tcgtcgtcgg tgtggttcag gacgaacaac cagctgcccg
    18961 agtcggaccg gcgtcgtacg acctcgatcc ccggtgaggt gcgcacgacc ggctcgacgc
//
LOCUS       DAOSOC010000028        19006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_2908_length_19006_cov_8.938526, whole genome shotgun sequence.
ACCESSION   DAOSOC010000028 DAOSOC010000000
VERSION     DAOSOC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 19006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19006
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_19006_cov_8.938526"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="PL014_03970"
     CDS             complement(<1..1481)
                     /locus_tag="PL014_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT56278.1"
                     /translation="MASLPSSARCVIIGAGIVGNSLAYHLAELGWTDIVQLDKGPLPN
                     PGGSTGHASNFIFPVDHSREFADITLDSVRQYKELGVFTESGGYEVARTEERMEELRR
                     RMSSAKAWGIPAELVTPEQVVEKVPFLDPSVIIGAAWFPTVGVVDSLRAGTIMRERAV
                     EKGALTTVPNVEVTGIEVVEGRIRAVETTQGRIEAETVVIACGVWSPKIARMAGARIP
                     LTPAVHQMISVGPCEVLAERPGEISYPIVRDMDTFCYERQHGSDMEVGSYAHRAILWD
                     PEDIPSIEQSKLSPTELPFTEDDFDPQLEQALELMPELLGDERAEIRYAINGLLSLTP
                     DGYPILGETPEVKGLWSAAAVWIKEGPGTARAVAEWMTHGNPEIDLGHSDIARFHPHQ
                     KTRAHVKARTSESFIKTYGIVHPGEQYESNRNIRLAPMHASERELGAVFYETAGWERP
                     FWYESNAGLVEKFGDACMPRENEWDSRWWSPIINAEHLQMRETA"
     assembly_gap    1778..1877
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1878..3076)
                     /locus_tag="PL014_03975"
     CDS             complement(<1878..3076)
                     /locus_tag="PL014_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HOT56279.1"
                     /translation="MADTLSTERVDQHGNSRRRQGVDKVVFGVAAGLALVFLLYGALD
                     SEGFGETGGSILTWITTNFGWFFVLTSGAFLLFSVFLAATRYGNIKLGADDSVPEFST
                     FSWVSMMFATGMGIGLMFYGVAEPLTHLNAPPMGMAEPGSEEAAHLAMEYTFFHWGFH
                     PWSMYAVIGLSIAYFAYRKGGGNLISAAFRPLLGDRVDGPVGQGINILAILATLFGSA
                     TSLGLGALQITGGLSNVFGNDGYGTTAAALVIAVLTVCFVISAVSGVDKGIKWLSNAN
                     AIAAGLLVFFLFVVGPTVFILSTFTESIGGYLTHLPTMSFRTGVFGGSEWLNGWTIFY
                     WAWWVSWTPFVGMFIARISKGRTIRQFVVFVIAVPSLVSFVWFSILGGAAFDLQLNEG
                     KDMAALV"
     gene            complement(3250..5283)
                     /gene="betT"
                     /locus_tag="PL014_03980"
     CDS             complement(3250..5283)
                     /gene="betT"
                     /locus_tag="PL014_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HOT56280.1"
                     /translation="MSNGARARAMAGPPVRVPVFAASLIGVLVMAIWALAAPTSAESA
                     LGSVTAWVTEWFGWFYVLLATAVLVFVLYLGVSRYGHIRLGPDHSRPEFSTFAWASML
                     FAAGIGTDVMFYSVVEPASQYMAPPVLEAETVEAARDATVWTLFHYGITGWGMYALMG
                     MALGYFCHRMGLPLAVRSALQPILGRRIEGPIGHAVDTAAVLGTIFGVATSLGIGVVF
                     LNVGLNLLFGVSVGTGAQIALAALAVIMAAVSATTGVDKGIRFLSQLNVLLALGLAGW
                     ILVTGNTSFILNAVVANVGDFARSFPAKTMETFPFVDNAEWMSYWTLFFWAWWVAWAS
                     FVGLFLARISRGRTIRQFVAGTMIIPFLYIVMWISIFGNATIERIRGGDAEFATIAQD
                     FTGAGFYELLKGYPAANVVIALAVFVGLLFYVTSADSGALVMANLCSRLETGSEDAAA
                     WQRILWAAVTGLLTIAMLIVGGIVALQYATIIFGLPFAFVLVLVMLGLLKALRREGRR
                     VDSGAHTMSAKLSARGSDGDAQPASLWKTRLARLTNFVDRADAQTHLDDVVRPALDDV
                     AEELGRLGVDTEVTPDLVGPEGSEQPAVTLRTLSEDEPFIYRVVLTEGPVPTYGGRMM
                     QARDTHARLEVHLEAGGQDYDVMGYSRGQVIHDCLDNYEQHLQFLRLGSSTKS"
     gene            complement(5330..7066)
                     /gene="betA"
                     /locus_tag="PL014_03985"
     CDS             complement(5330..7066)
                     /gene="betA"
                     /locus_tag="PL014_03985"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HOT56281.1"
                     /translation="MKLRMPDRASEALARTTLGAAPDRVDVVVVGGGSAGCVIANRLS
                     ADAGTSVLVLEAGRADLKIDPFIHMPAALPFPIGSRFYDWKYESEPEPHMNGRRIYHA
                     RGKVLGGSSSINGMIFQRGNPMDYERWASDPGMESWDFAHCLPYFKKMETCRAGADEW
                     RGGSGPLILERGPATNPLFGAFFAAAVEAGYPLTDDVNGYRQEGFAKFDRNINRGRRW
                     SAARAYLHPVMNRPNLHVRTLAMTTRILFEGTRAVGVEYTQLGATHTVRAGEVVLCGG
                     AINTPQLLQLSGVGNADELKALGITPVADVKGVGEHLQDHLEVYIQYASKQPVSIAPG
                     LKYHNRPKLAADWLFFRKGLGATNHFEGGGFVRSNDDVDYPNLMFHFLPIAIRYDGSS
                     PIKGHGYQVHIGPMYSDARGTIKITSTNPMDKPAMRFNYLSTDQDRREWVESIRVARH
                     ILGQSAMDPFNDGEISPGPSVETDEEILDWVRKDAETALHPSCTARMGVDDMSVLDPL
                     TMAVHGTQGLRVVDASAMPYVTNGNIYAPVIMLAEKAADLIAGNTPLPPSDAPWYNHR
                     AGSPLHAPTKAT"
     gene            complement(7063..8559)
                     /locus_tag="PL014_03990"
     CDS             complement(7063..8559)
                     /locus_tag="PL014_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT56282.1"
                     /translation="MVQPVGGAVAELFIDGQWRPAADGATRDIRCPADGSFVATVAEA
                     SEVDTLAAIAAARAAFDDGDWSTTPMSTRGGVLAKVADLLERDAAQIARAEALDTGKR
                     YVEAEYDVADVVSVFRHYAKVASTASDAGRVADVGREGVESTIRKEPIGVCALITPWN
                     YPLLQASWKVAPALAAGDTFVLKPSEITPHSSVHLIRILDEAGVPAGVANLVLGTGAG
                     CGGPLSSDPRVDLVSFTGSLAVGRHLMAAAAPTVKRVALELGGKNPNIVFADADREAA
                     LDNAMTAVFLHSGQVCSAGARLIVEESIAEEFVDEIIARARGIRLGGPFEDAAQTGPL
                     TSEAHLRKVEAYVAAGIAEGARLRLGGERATEGDLADGYYFPPTVFDRCRSDMSIVQE
                     ESFGPVLTVETFTTDDEAVALANDSIFGLAGAIWTTDQSRADRIARRLRMGTVWINDY
                     HPYVAQAEWGGYKQSGIGRELGELGLEEYQETKHVWRNTAPAPSGWWT"
     gene            complement(8661..9443)
                     /locus_tag="PL014_03995"
     CDS             complement(8661..9443)
                     /locus_tag="PL014_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT56283.1"
                     /translation="MRTTNRPASGTVQSVDRAVTILELLAVHGTAGVTQLARELGVHK
                     STASRLVAALERRRLVEQVEDRGHYRLGVGVLKLAGATTARLDLAQEARPVCRRLAAD
                     TGETVNLAVLSGGAVLYVDQVSGTSTLSSYNWVGQHIPIHASSNGKVLVSELDGTELT
                     RMLGELVAYTPSTVTDRARLEEELAQVRQRGFAIATDELDLGLTAVAAPVRDAHGDVV
                     ASVSVSGPSFRFDEARIAEILPLVRTATTEISSRLAHVPPER"
     gene            9491..10378
                     /gene="purU"
                     /locus_tag="PL014_04000"
     CDS             9491..10378
                     /gene="purU"
                     /locus_tag="PL014_04000"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOT56284.1"
                     /translation="MTSTPATTATAPTTDAVLVLSCPDQRGIVAAVSALLFDHGANIE
                     ESQQFDDVRTDQFFMRIRFSVSAGGPDVGRWRELFADVAARFDMQWQIRGVADPYRAL
                     IMVSRFGHCLNDLLFRWRSGALTMDIPAVVSNHRDLEPLVTSYGIPFHHVPVTADTKS
                     QAEARLREIVAEHDVDLVVLARYMQVLSDDLSRDLSGRVINIHHSFLPSFKGAKPYHQ
                     AFARGVKLVGATAHYVTADLDEGPIIDQDVVRVDHRMAAEDLVRAGEEVESRVLARAV
                     RWHCESRVLLNGDRTVVFS"
     gene            complement(10430..10573)
                     /locus_tag="PL014_04005"
     CDS             complement(10430..10573)
                     /locus_tag="PL014_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56285.1"
                     /translation="MITPGIAASLTPTSVERTPVAPRPRRRRHLPHVRLHLPHLHLPH
                     RHA"
     gene            10702..11136
                     /locus_tag="PL014_04010"
     CDS             10702..11136
                     /locus_tag="PL014_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56286.1"
                     /translation="MSFHDLPENWSDLPLDTPGLAADVADLVVGDEDRANGCVGLILV
                     GPDRRMAQPCLVNEVDDTVDPQEFAPFLAQLCGMVADTDGSMIFVRGRSGPALLTDVD
                     RRWHQIATDACRNGGVTLLGAFLAADSGVRAFPQAWTDVRAS"
     gene            complement(11155..12540)
                     /locus_tag="PL014_04015"
     CDS             complement(11155..12540)
                     /locus_tag="PL014_04015"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOT56287.1"
                     /translation="MAISAFDLFSVGIGPSSSHTVGPMRAAHTFAEGLRTDGLLPRVG
                     RVRAELFGSLGATGHGHGSVKAVVLGLEGEQPEHTDPRGADARLDAVVRDGILQLAGE
                     HGIRCDVADDVVLHRRATLPFHANGMRFAAYASAEPAEDEAPLRQREYYSVGGGFVLD
                     EDEVGENKIVPDHTPVRYPFTTGDQLLARCAETGLPISGVMLANELSWRTEAEVREGL
                     LHLWQVMQECVDNGCRAEGVLPGGLKVQRRAPRLARQLRTEHPGDPLAAMDWVTLYAL
                     AVNEENASGGRIVTAPTNGAAGIIPAVLHYYTRFVPGADDDGVVSFLLTAAAIGSLYK
                     ENASISGAEVGCQGEVGSACSMAAGALAEVLGGTPDQVENAAEIGIEHNLGLTCDPVG
                     GLVQIPCIERNAVASVKAITAARIALRGTGRQVVSLDKAIKTMRDTGRDMKVKYKETS
                     RGGLAVNVIEC"
     gene            complement(12556..13995)
                     /locus_tag="PL014_04020"
     CDS             complement(12556..13995)
                     /locus_tag="PL014_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOT56288.1"
                     /translation="MFTNRMPRYEILSADALAQLDAGWRRLVTEIGVEFMSDRALDLF
                     RAAGQRVEDNTVFLDPEFVLEQVAKAPREFDVHARNAERSIHIGGDSMAFGAVYGPPF
                     VREGDVRRDGTLEDYRNFAKLSQSFSVLDSAGGVVCEPNDAPLDSRHLDMTLSLMTLT
                     DKVFMGNVVSGENARDVIAMSEILFGGREAIEARPASISLINCNSPLRWDDRMLDALF
                     EYCAAAQPVVLTPFILMGAMSPVTIPAALVQQMAEALSGIALAQLIRPGCPVIFGSFL
                     SNIDMQSGSPTFGTPESGIGLLCTGQIARHFNLPFRSGGGLTSSQVPDAQAGYEALMT
                     LMPTFLAGTNWVMHTAGWLEGGLVAGFEKFVIDCQIVEMLQHEFTPLEIDPGSLAFDA
                     HQEVGHGGHFLGSMHTMERFRTCFYRPFLNSSDNYERWMRNGAQDTATRAAGIWRKRL
                     EEYEEPTLDDGIRSELEAFVTRRRRELGD"
     gene            14133..14900
                     /locus_tag="PL014_04025"
     CDS             14133..14900
                     /locus_tag="PL014_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT56289.1"
                     /translation="MTTPTVGTGTQSIDRAADLLARVVRAPSPVTFTELAEVTGLARS
                     TTSRLLSALERADLLARDEQARWIPGALFDHYAARRSDDAHLVEAADSTMRALGDLTG
                     ETVNLGVPRRGMVVQVAQVDSSYYLGSRDWVGTDVPAHCSALGLVLYAHGALPAPSGK
                     LEALTPASLATGAALKARLPGIRHDGFSTTVDELEPGLTGVAAPVRLGGTVVAALGIS
                     GPTSRLAADLRSTGTLVAAHAKALSNRLDHHHQEGAA"
     gene            14897..15562
                     /locus_tag="PL014_04030"
     CDS             14897..15562
                     /locus_tag="PL014_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOT56290.1"
                     /translation="MTPDEILQGLYDQTLVGNGPAVLDLTHQALDGGMEPGTMLFDAL
                     IPALEEVGARFERGDFFVPEMLIAGRAMAGSMEVLRPLLAETGVETIGKFLMGTVKGD
                     VHDIGKNLVNIMLEGAGFEVIDLGVQVAPEKFVSAIEEHQPDIVGFSAFLTTTMPMFK
                     ANINALQKAGLRDSVIVMVGGAPVTQEYADAVGADGYASDASATVKRAKALLEARRTK
                     VPA"
     gene            15559..16482
                     /locus_tag="PL014_04035"
     CDS             15559..16482
                     /locus_tag="PL014_04035"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT56291.1"
                     /translation="MSAPLRRTVLRSASKEVVIAADQPFCVIGERINPTGRPVFQKAL
                     REGDLSIIEKDVAAQVAGGANVLDINMGVPLTDEADLLVRAIQLVQQVTDLPICIDSS
                     VVEALEAGLAAYQGRALVNSVTAEDERLEAILPLVKKYGAAIVALPNDEHEIPMEHQR
                     RLELTEKIVRVATQEYGIAIDDIVIDPLAMPIGADPTVGRETLLTIRGIVEQWGLNTT
                     CGASNVSFGMPGRHAINGAWLSLAMSAGMTSAIMDARTPHVVDAVRASDLLLGNDEWG
                     SAWITTHRARQAAAAAEAAAAEAAAASTASA"
     gene            16479..18449
                     /locus_tag="PL014_04040"
     CDS             16479..18449
                     /locus_tag="PL014_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOT56292.1"
                     /translation="MSDGRGPGAPFGDVVDGLRREGLLEAPPPAAEVGEPHEGTGRVQ
                     LTFEPAGRTFRVPPGVSVFDAASWNGIAIDSTCGGHGTCRKCLVRVTSGSAPVTRHDR
                     RTFSEDQIAEGWRLACLVKATHDLGLEVPPLVTRPKASTVGVGRQVILRPGVQKRYVE
                     LTDPTLADQRTDLQRLRDAVDDLTLNPDLHALRRLPTVLRQSDFKVTAVVVDEVLIDV
                     EPGDTTGSRHAIAYDLGTTTVVATLLDLETGTPVAVASMLNKQQPFGGDVITRISATM
                     MDPEALGRLTQLARQTLSELAGEVLDEGGISPQSVYEVALAGNATMTALLLGIDPEPL
                     GVAPFIQVSADWPVLHASDLGIDVHPGARATIFPALGAYVGGDIVAGMLASGLDRDKR
                     VRLFIDVGTNCEIALSDGERIVTTAAPAGPAFEGGAIRCGMRAADGAIEVITLNAGAS
                     GDDEAVTLGVIGDAEPRGICGSGLVDAVAELVRVGLLDESGRLISDEAAAASVPALAG
                     RLATIGDGERVFILHRPTPDAPTVECVYLSQRDVRELQFAKAAISTGWTLLLEELGVS
                     PRDVQQVLLAGSFGSYLSPSSAVRIGLVPKLPVLRIVSAGNVAGEGAKMVLLSARERA
                     GADAMLADVAYIELSDRPDFNDRFIDQLAFPV"
     gene            18452..>19006
                     /locus_tag="PL014_04045"
     CDS             18452..>19006
                     /locus_tag="PL014_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOT56293.1"
                     /translation="MGVPSGRVALVACGAIAQPAADAVARRAWPVDVHPLPPLLHNRP
                     ERIADAVEALVLQLRGRYDRVVVGYADCGTYGALDAVCERLHVDRLPGLHCYDLFAGS
                     DVVSRLSEDEPGTYLLTDFLVKSFARTVIQELGLDRHPELRDDYFRHYTRVVWLVQTA
                     DPVERAELRTLAQAAADRIGLPLQV"
BASE COUNT         2844 a         6408 c         6791 g         2863 t
ORIGIN      
        1 gcggtctcgc gcatctgcag gtgctcggcg ttgatgatcg gcgaccacca gcgcgagtcc
       61 cactcgttct cgcgcggcat gcacgcgtcg ccgaacttct cgacgagccc ggcgttcgac
      121 tcgtaccaga acgggcgctc ccagccggcg gtctcgtaga acaccgcgcc gagctcgcgc
      181 tcggaggcgt gcatgggggc caggcggatg ttgcggttgc tctcgtactg ctcgccgggg
      241 tgcacgatgc cgtaggtctt gatgaacgac tcggaggtgc gcgccttgac gtgcgcccgg
      301 gtcttctggt gcgggtggaa gcgcgcgatg tcggagtggc cgaggtcgat ctcggggttg
      361 ccgtgcgtca tccactcggc gacggcgcgg gcggtgcccg gaccctcctt gatccacacc
      421 gcggcggccg accacaggcc cttgacctcg ggcgtctcac cgaggatcgg gtagccgtcg
      481 ggggtcagcg agagcaaccc gttgatggcg tagcggatct cggcgcgctc gtcgccgagg
      541 agctcgggca tgagctcgag cgcctgctcg agctgcgggt cgaagtcgtc ctcggtgaac
      601 ggcagctcgg tgggggagag cttgctctgc tcgatggagg ggatgtcctc cgggtcccag
      661 aggatggcgc ggtgggcgta ggagccgacc tccatgtccg agccgtgctg gcgctcgtag
      721 cagaaggtgt ccatgtcgcg aacgatcggg tagctgatct caccgggacg ctcggcgagc
      781 acctcgcacg ggccgaccga gatcatctgg tgcaccgccg gggtgagggg gatgcgcgcg
      841 ccggccatgc gggcgatctt ggggctccag acaccgcagg cgatgacgac cgtctccgcc
      901 tcgatgcggc cctgggtggt ctcgaccgcg cggatgcgac cctcgaccac ctcgatgccc
      961 gtgacctcga cgttcgggac ggtcgtcagg gcgcccttct cgacggcgcg ctcgcgcatg
     1021 atggtgccgg cgcgcaggct gtcgacgacg ccgacggtcg ggaaccaggc agcaccgatg
     1081 atgacgctcg ggtcgaggaa cgggaccttc tcgacgacct gctcgggggt gaccagctcg
     1141 gcggggatgc cccaggcctt ggcgctgctc atccggcggc gcagttcctc catgcgctcc
     1201 tcggtgcgcg cgacctcgta gccgccggac tcggtgaaga cgcccagctc cttgtactgg
     1261 cgcacggagt ccagcgtgat gtcggcgaac tcgcgcgagt ggtcgaccgg gaagatgaag
     1321 ttgctcgcgt ggcccgtgga gccaccgggg ttgggcaacg ggcccttgtc gagctggacg
     1381 atgtcggtcc agccgagctc ggccaggtgg taggcgagcg agttcccgac gatgccggcg
     1441 ccgatgatga cgcagcgggc ggaagaagga agggaggcca tccgtggagt cctttctgac
     1501 ggcatccggg gtgcgtatga cgcaacctga cgctcgatgt gcaacgcggc caccagtgtg
     1561 gcagatcaca gggggtgtgc caacacctca cgcggccgcg ccgcccgagg gccggatggc
     1621 ggactcgggc ggcgcggtgg tgcggcggag gtgatgcggg ctgaccgcag gggatgccgg
     1681 tggctcagct accgtcgcga acgtcgtcgt ggtgtttggt cgcgacgacc accttgcgca
     1741 gcgggtcacg cagggttgcc tcgaaggtct cctcgcgnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnntcg acgagggccg ccatgtcctt gccctcgttg agctgcaggt
     1921 cgaaggcggc accaccgagg atcgagaacc acacgaagga cacgagcgac ggaacggcga
     1981 tcacgaagac gacgaactgg cggatcgtgc ggcccttgga gatgcgggcg atgaacatgc
     2041 cgacgaaggg ggtccaggag acccaccagg cccagtagaa gatcgtccac ccgttgagcc
     2101 actcgctgcc accgaacacg ccggtgcgga agctcatcgt cggcaggtgg gtgaggtagc
     2161 caccgatgga ctcggtgaag gtcgagagga tgaagacggt cgggccgacc acgaacagga
     2221 agaacacgag caggccggcg gcgatggcgt tggcgttgga gagccacttg atgcccttgt
     2281 cgacgccact gaccgcggag atgacgaagc acacggtcag gacggcgatg accagtgctg
     2341 cggcggtggt gccgtagccg tcgttgccga agacgttgga gaggccgccg gtgatctgca
     2401 gcgctccgag gccgagcgag gtggccgacc cgaacagggt ggccaggatc gcgaggatgt
     2461 tgatgccctg gccgacggga ccgtccaccc ggtcaccgag gaggggccgg aaggcggcgc
     2521 tgatgaggtt gccgccaccc ttgcggtagg cgaagtaggc gatcgacagg ccgatcacgg
     2581 cgtacatcga ccacgggtgg aagccccagt ggaagaacgt gtactccatc gcgagatggg
     2641 cggcttcctc gctaccgggc tcggccatgc ccatcggcgg ggcgttgagg tgcgtcaacg
     2701 gctccgcgac gccgtagaac atcaggccga tgcccatccc ggtcgcgaac atcatcgaca
     2761 cccacgagaa ggtcgagaac tccggcaccg agtcgtcggc gccgagcttg atgttgccgt
     2821 agcgggtcgc cgcgaggaac acgctgaaga gcaggaacgc gccgctggtg agcacgaaga
     2881 accacccgaa gttcgtcgtg atccacgtga ggatgctgcc gcccgtctcg ccgaagccct
     2941 cgctgtccag tgccccgtag aggaggaaga ccagggccag ccctgcggcg acaccgaaga
     3001 cgaccttgtc caccccctgg cgccgcctgc tgttgccgtg ttggtccacg cgttcggtgc
     3061 tcaaggtgtc tgccatgaac tctccctcgg tcgtggttgc ccacctcgca acccgtcgcg
     3121 tcctccgcaa ctgggatcga gtgtgacccc ggtcatgcgg cccgtcaacc gctgggttcg
     3181 cgacacgccg gatgccgggg cgccacgcga cgtggcaccc cggcatccgg ccccctcctg
     3241 tccggtgggt cagctcttgg tggaactgcc cagacggagg aactggaggt gctgttcgta
     3301 gttgtccagg cagtcgtgga tgacctgccc ccggctgtat cccatgacgt cgtagtcctg
     3361 accaccggcc tcgaggtgca cctcgaggcg ggcatgggtg tcgcgggcct gcatcatccg
     3421 tccgccgtac gtcgggacgg gcccctccgt gaggaccacc cggtagatga acggctcgtc
     3481 ctcggacagc gtgcgcaggg tgactgcggg ctgctcggag ccctcggggc cgaccaggtc
     3541 cggcgtgacc tcggtatcca ctccgagtcg cccgagttcc tcggcgacgt cgtccagggc
     3601 tggtcgaacg acgtcatcga gatgggtctg ggcgtccgct cggtccacga agttggtcag
     3661 ccgcgccaac cgggtcttcc acagggatgc cggctgggcg tcaccgtcac tgccgcgggc
     3721 ggacagcttc gccgacatgg tgtgggcgcc gctgtcgacc cggcgtccct cacgccgcag
     3781 ggccttgagg aggccgagca tcacgaggac caggacaaac gcgaagggca gaccgaagat
     3841 gatcgtcgcg tactgcaggg ccacgatgcc gcccacgatg agcatcgcga tcgtgagcag
     3901 accggtcact gccgcccaca ggatgcgctg ccacgcggcg gcgtcctccg acccggtctc
     3961 cagccggctg cacaggttcg ccatgacgag cgctccggag tcagccgagg tgacgtagaa
     4021 gagcagaccg acgaagacgg caagggcgat gacgacgttg gccgccgggt aacccttcag
     4081 gagctcgtag aatccggctc cggtgaagtc ctgcgcgatg gtggcgaact cggcatcccc
     4141 gccgcggatc cgctcgatgg tggcgttgcc gaagatcgag atccacatga cgatgtagag
     4201 gaacgggatg atcatcgttc cggcgacgaa ctggcggatc gtgcgcccgc gcgagatgcg
     4261 cgccaggaac agcccgacga acgatgccca ggcaacccac caggcccaga agaacagggt
     4321 ccagtagctc atccactcgg cgttgtcgac gaacgggaag gtctccatcg tcttggccgg
     4381 gaaggagcgg gcgaaatcgc cgacgttggc gacgacggcg ttgaggatga agctcgtgtt
     4441 gccggtgacg aggatccagc cggccagccc gagggccagc agcacgttga gctgggagag
     4501 gaaccggatg cccttgtcga cgcccgtcgt ggccgacacg gcggccatga tgacggcgag
     4561 cgcggcgagg gcgatctggg cgccggtgcc gaccgagacc ccgaacaaca ggttcaggcc
     4621 gacgttgagg aagacgacgc cgatcccgag ggaggtggcg acgccgaaga tcgtgccgag
     4681 cacagccgcc gtgtcgaccg cgtggccgat gggcccttcg atgcggcgac cgaggatcgg
     4741 ctggagcgcc gaacggacgg ccaggggtag ccccatgcgg tgacagaaat accccagggc
     4801 cattcccatg agggcataca tgccccaccc ggtgatgccg tagtggaaca gcgtccacac
     4861 cgtcgcgtca cgggcagcct cgacggtctc ggcctcgagg acgggaggag ccatgtactg
     4921 gctggccggc tcgacgacgg agtagaacat gacgtcggtg ccgatacccg ccgcgaacaa
     4981 catcgaggcc caggcgaagg tggagaactc ggggcgcgag tggtccgggc ccaaccggat
     5041 gtggccgtac cgcgacaccc cgaggtagag cacgaagacc aggacggcgg tcgcgagcag
     5101 gacgtagaac cagccgaacc actcggtgac ccaggccgtc accgacccga gggcgctctc
     5161 ggcagaggta ggggcagcca gtgcccagat cgccatgacg agcacgccga tcagggaggc
     5221 agcgaagacg ggaacacgga ccggcggtcc ggccatggcg cgggctcgag cgccgttgct
     5281 catgagacct ccagggattg tcgcggtggg gatgcccgtg agtggggact caggtggcct
     5341 ttgtgggggc gtggagcggg ctgcccgcgc ggtggttgta ccagggcgcg tcggatggcg
     5401 ggaggggggt gtttcccgcg atgaggtcag ccgccttctc ggcgagcatg atgaccgggg
     5461 cgtagatgtt gccgttggtc acgtagggca tggcgctggc gtcgacgacc cgcaggccct
     5521 gggtgccgtg caccgccatg gtgagcgggt cgagcaccga catgtcatcc acgcccatgc
     5581 gagcggtgca gctcgggtgc agggcggtct cggcgtcctt gcggacccag tcgaggatct
     5641 cctcgtcggt ctcgacgctc gggccgggag agatctcacc gtcgttgaac gggtccatcg
     5701 cgctctgccc gaggatgtgg cgggcgacgc ggatcgattc gacccactct cgacgatcct
     5761 ggtcggtgga caggtagttg aaccgcatcg ccggcttgtc catcgggttg gtgctcgtga
     5821 tcttgatggt gccgcgggcg tcggagtaca tcgggccgat gtgcacctgg tagccgtggc
     5881 ccttgatcgg gctcgacccg tcgtagcgga tggcgatcgg caggaagtgg aacatcaggt
     5941 tggggtagtc gacgtcgtcg ttgctgcgca cgaagccacc accctcgaag tggttggtcg
     6001 cgcccaggcc cttgcggaag aagagccagt cggcggccag cttgggccgg ttgtggtact
     6061 tcagccccgg tgcgatggac accggttgct tgctcgcgta ctggatgtac acctcgaggt
     6121 ggtcctggag gtgctcgccg acccccttga cgtcggccac gggcgtgatg ccgagtgcct
     6181 tgagctcgtc ggcgttgccg accccggaca gctggagcag ctgcggggtg ttgatcgcgc
     6241 cgccgcacag cacgacctcg cccgcgcgca cggtgtgggt ggccccgagc tgggtgtact
     6301 cgaccccgac cgcccgggtg ccctcgaaga ggatccgggt ggtcatggcc agggtgcgca
     6361 cgtgcaggtt cggacggttc atgaccgggt gcagataggc gcgggccgcg gaccaccgac
     6421 ggccgcggtt gatgttgcgg tcgaacttcg cgaagccctc ctgccggtag ccgttgacgt
     6481 catcggtgag gggatagccc gcctcgacgg ccgcggcgaa gaaggcaccg aacagcgggt
     6541 tggtggcggg tccacgttcc aggatgagcg ggccgctgcc accgcgccac tcgtcggcgc
     6601 cggcgcggca ggtctccatc ttcttgaagt agggcaggca gtgggcgaag tcccaggact
     6661 ccatgcccgg gtcggaggcc cagcgctcgt agtccatcgg gttgccccgc tggaagatca
     6721 tcccgttgat cgagctcgac ccaccgagca ccttgccgcg ggcgtggtag atccgacggc
     6781 cgttcatgtg cggctcgggc tcggactcgt acttccagtc gtagaaccgg ctgccgatgg
     6841 ggaacggcag cgccgccggc atgtggatga acgggtcgat cttcaggtcg gcccggccgg
     6901 cctcgaggac cagcaccgag gtcccggcgt cggccgacaa ccggttggcg atgacgcacc
     6961 cggcgctgcc gccgccgacg acgacgacgt cgacccggtc cggtgccgcg cccagcgtcg
     7021 tcctggccag cgcctcactg gcgcgatccg gcatccgaag tttcatgtcc accatcctga
     7081 gggggcgggc gcggtgttgc gccacacgtg tttggtctcc tggtactcct cgaggccgag
     7141 ctcaccgagc tcacgcccga tgccggactg cttgtagccg ccccactccg cctgggcgac
     7201 gtaggggtgg tagtcgttga tccagaccgt gcccatgcgc aggcggcggg cgatgcggtc
     7261 ggcccgggac tggtcggtgg tccagatggc gcccgcgagc ccgaagatcg agtcgttcgc
     7321 cagtgccacc gcctcgtcgt cggtggtgaa ggtctcgacg gtcagcaccg gcccgaacga
     7381 ctcctcctgc acgatgctca tgtcgctgcg gcaccggtcg aacacggtgg gcgggaagta
     7441 gtagccgtca gcgaggtcac cctcggtcgc gcgctcgcca cccaggcgca gccgggcgcc
     7501 ctcggcgatc cccgcggcga cgtaggcctc caccttgcgc aggtgggcct cgctcgtcag
     7561 cggcccggtc tgggccgcgt cctcgaacgg gccgccgagc cggatgccgc gggcgcgcgc
     7621 gatgatctcg tcgacgaact cctcggcgat ggactcctcg acgatcagcc gcgccccggc
     7681 ggagcagacc tggccggagt gcaggaagac cgcggtcatc gcgttgtcga gggctgcctc
     7741 gcgatcggcg tcggcgaaga cgatgttggg gttcttgccg ccgagttcca gcgccacccg
     7801 cttgaccgtc ggtgcggccg ccgccatgag gtgtcgcccg acggccaggc tgccggtgaa
     7861 cgagaccagg tcgacgcggg ggtcgctgga cagcggcccg ccgcagccgg cgccggtgcc
     7921 gagcacgagg ttcgcgactc ctgcgggcac gccggcctcg tcgaggatgc ggatcaggtg
     7981 cacgctggag tgcggggtga tctcgctggg cttgagcacg aaggtgtcgc cggccgcgag
     8041 tgccggggcg accttccagc tggcctgcag gagggggtag ttccacggcg tgatgagggc
     8101 acacacgccg atcggttcct tgcggatcgt gctctcgaca ccctcgcgcc cgacgtcggc
     8161 gacgcgaccg gcgtccgatg ccgtggacgc gaccttcgcg tagtggcgaa agaccgagac
     8221 gacgtcagcg acgtcgtact cggcctcgac gtagcgcttg ccggtgtcga gcgcctcggc
     8281 gcgggcgatc tgcgcagcat cgcgctcgag caggtcggcg accttggcga gcaccccgcc
     8341 tcgtgtcgac atcggcgtgg tcgaccagtc gccgtcgtcg aaggcggcgc gggctgcggc
     8401 gatggcggcg agggtgtcga cctcgctggc ctcggcgacg gtggcgacga acgagccgtc
     8461 tgcggggcag cggatgtcgc gtgttgcgcc gtccgcagcc ggtcgccact gaccgtcgat
     8521 gaacagttcc gccacggcac ctcccactgg ttgcaccatg tgcaacatcg atgtgatgtg
     8581 cgcaacacaa tggtgcagga gagggtggcg gtcaagccgc ttcgctgaat tgggcgatcc
     8641 gacgtgccgt cggcgtgccc tcagcgctcc ggggggacgt gcgcgaggcg gctgctgatc
     8701 tcggtcgtcg cggtgcgcac gagggggagg atctcggcga tccgggcctc gtcgaagcgg
     8761 aaggaggggc cggacacgct gaccgacgcg acgacgtcgc cgtgcgcgtc ccgcacgggt
     8821 gccgccaccg cggtcagacc gaggtcgagc tcgtcggtgg cgatggcgaa gccgcgctgg
     8881 cgtacctgcg ccagttcctc ttccagacgt gcccgatcag tgacggtcga cggggtgtag
     8941 gccacgagct caccgagcat gcgggtcagt tcggtgccgt cgagttcgct gacgaggacc
     9001 ttgccgttgc tcgacgcgtg gatcgggatg tgctggccga cccagttgta cgaggacagg
     9061 gtcgaggtgc ccgagacctg gtcgacgtag agaaccgcac cacccgacag gacggccagg
     9121 ttgaccgtct caccggtgtc ggccgcgagg cggcggcaca cggggcgggc ttcctgggcc
     9181 aggtcgagac gggccgtcgt ggcgcctgcg agcttgagca ccccgacccc gaggcggtag
     9241 tggccgcggt cctcgacctg ttcgacgagg cgccgccgct cgagggcggc gaccagccga
     9301 ctcgccgtcg acttgtggac cccgagttcg cgggcgagct gtgtcacgcc tgcagtgccg
     9361 tggacggcga gcagctcgag gatggtcacc gcgcggtcga cggactgcac cgtgcccgac
     9421 gccggtcggt ttgtggtgcg catggcggaa cagtatcggg tggttcggct cggccgtcta
     9481 gcgtgggggt atgaccagca cccccgcgac cactgcgacc gccccgacca cggatgccgt
     9541 tctcgtcctg tcctgccccg atcagcgcgg catcgtcgcc gcggtgtcgg cgctgctgtt
     9601 cgaccacggt gcgaacatcg aggagtcgca gcagttcgac gacgtgcgca ccgaccagtt
     9661 cttcatgcgg atccgcttct cggtctccgc cgggggcccg gacgtcgggc gctggcgcga
     9721 gctcttcgcc gacgtcgccg cgcgcttcga catgcagtgg cagattcgcg gcgtcgccga
     9781 cccctaccgt gccctgatca tggtcagccg tttcgggcac tgcctcaacg acctgctctt
     9841 ccgctggcgt tccggtgcgc tgacgatgga catcccggcg gtggtgtcca accaccgcga
     9901 cctcgaacca ctcgtgacgt cgtacggcat cccgttccac cacgtcccgg tcacggcgga
     9961 caccaagtcg caggcggagg cccgcctgcg cgagatcgtc gccgagcacg acgtcgacct
    10021 cgtcgtgctg gcccgctaca tgcaggtgct ctccgacgac ctgtcacgtg acctgtccgg
    10081 ccgggtcatc aacatccacc actcgttcct gccctcgttc aagggcgcca agccctacca
    10141 ccaggcgttc gcgcggggcg tcaaactcgt cggggcgacg gctcactacg tgacggcgga
    10201 cctcgacgag gggccgatca tcgaccagga cgtcgtgcgc gtggaccacc ggatggccgc
    10261 cgaggacctc gttcgtgccg gcgaggaggt cgagtcgcgg gtgctggcgc gagcggtgcg
    10321 ctggcactgc gagtcgcggg tgctgctcaa cggcgaccgg acggtcgtct tcagctgacg
    10381 cgcacgccgt cgggccggtg cccgactcct cgaccggggc cgggaggtct caggcgtggc
    10441 ggtgcggcag gtgcaggtgc ggcaggtgca gccgcacgtg cggtaggtgg cgacgccgtc
    10501 gcggccgcgg ggcgaccggg gtgcgctcga cggacgtggg ggtcagggat gcggcgatgc
    10561 cgggcgtgat catgtgcatg tccctcagga ggtcccggcg gcgcccctga taccggtgcc
    10621 cggcgtggtc tcgtggcgac ggtcaccgaa ccggcctgtg gacaactcgg agaggcccgc
    10681 cgggacgggc agggtggggc catgagcttc cacgacctcc ccgagaactg gtccgacctc
    10741 ccgctcgaca cgcccggcct ggcggcagac gtcgccgacc tggtcgtggg ggacgaggat
    10801 cgagccaacg gctgcgtcgg cctgatcctg gtgggccccg accgccggat ggcgcaaccc
    10861 tgcctggtca acgaggtcga cgacacagtc gatcctcagg agttcgcgcc gttcctcgcg
    10921 caactgtgcg gcatggtcgc ggacaccgac gggtcgatga tcttcgtgcg cgggcgctcg
    10981 ggtcccgccc tgctcaccga cgtcgatcga cgctggcacc agatcgccac cgacgcctgc
    11041 aggaatggcg gcgtcacgct ccttggtgcc ttcctcgccg ccgactcagg ggtgcgggca
    11101 tttccgcagg cgtggaccga cgtgcgcgca tcgtgatggg gtcggccccc gcgctcagca
    11161 ctcgatgacg ttgacggcga gcccgccgcg cgaggtctcc ttgtacttga ccttcatgtc
    11221 gcgaccggtg tcgcgcatcg tcttgatcgc cttgtccagt gagacgacct gacgcccggt
    11281 gccccgcagc gcgatccggg cggcggtgat cgccttgacc gacgccaccg cgttgcgctc
    11341 gatgcacggg atctgcacga gcccacccac ggggtcgcag gtgagcccga ggttgtgctc
    11401 gatgccgatc tcggccgcgt tctcgacctg gtcgggggtc ccgccgagca cctcggccag
    11461 ggcgccggcc gccatcgagc aggccgagcc gacctcgccc tggcagccga cctccgcgcc
    11521 ggagatcgac gcgttctcct tgtagaggct cccgatcgcg gcggcggtga gcaggaagct
    11581 cacgacgccg tcgtcgtcgg caccgggcac gaagcgggtg tagtagtgca ggaccgcggg
    11641 gatgatgccc gccgctccgt tggtcggggc ggtcacgatg cgcccgccgg acgcgttctc
    11701 ctcgttgacg gccagggcgt acagcgtgac ccagtccatg gccgcgagcg ggtccccggg
    11761 gtgctcggtg cgcagctggc gcgcgagccg cggggcccgg cgctgcacct tgagaccgcc
    11821 ggggagcaca ccctcggcgc ggcacccgtt gtcgacgcac tcctgcatga cctgccacag
    11881 gtgcagcagg ccctcgcgga cctcggcctc ggtgcgccac gagagctcgt tggcgagcat
    11941 cacgccgctg atcggcaggc cggtctcggc gcaccgggcg agcagctggt ccccggtggt
    12001 gaacgggtac cgcacggggg tgtggtcggg aacgatcttg ttctcgccca cctcgtcctc
    12061 gtccagcacg aatccgcccc cgacggagta gtactcgcgc tggcgcagcg gtgcctcatc
    12121 ctcggcgggc tcggcgctcg cgtacgccgc gaaccgcatg ccgttggcgt ggaagggcaa
    12181 cgtggcgcgg cggtgcagca ccacgtcgtc ggcgacgtcg cagcggatgc cgtgctcgcc
    12241 cgccagctgc aggatgccgt cgcgcaccac cgcgtccagg cgcgcgtcgg cgccccgtgg
    12301 gtcggtgtgt tcgggctgct cgccctccag gcccaggacc acggccttga ccgacccgtg
    12361 gccgtggccg gtggcgccca gggagccgaa aagctcggcg cgcacccggc cgacgcgggg
    12421 gaggaggccg tcggtgcgca ggccttcggc gaaggtgtgc gcggcccgca tcgggcccac
    12481 cgtgtgggag ctggacgggc cgatgcccac cgagaagagg tcgaacgcgc tgatggccat
    12541 ggcccagagc ctgcctcagt cgccgagctc ccggcggcgc cgggtcacga aggcctcgag
    12601 ctcggaccgg atgccgtcgt cgagcgtcgg ctcctcgtac tcctcgaggc gcttgcgcca
    12661 gatgccggcg gcgcgggtcg cggtgtcctg ggcaccattg cgcatccagc gctcgtagtt
    12721 gtccgaggag ttgaggaagg ggcggtagaa gcaggtgcgg aagcgctcca tcgtgtgcat
    12781 cgagcccagg aagtggccac cgtggcccac ttcctggtgg gcgtcgaacg ccagcgaacc
    12841 cgggtcgatc tccagcgggg tgaactcgtg ctggagcatc tcgacgatct ggcagtcgat
    12901 gacgaacttc tcgaagccgg cgaccagacc accttcgagc cagcccgcgg tgtgcatgac
    12961 ccagttggtg cccgcgagga acgtcggcat gagggtcatc agcgcctcgt aaccggcctg
    13021 cgcgtcaggg acctgggacg aggtgagccc gccaccggaa cggaacggca ggttgaagtg
    13081 gcgggcgatc tgcccggtgc acagcagccc gatgccggac tccggggtgc cgaacgtcgg
    13141 cgagcccgac tgcatgtcga tgttggacag gaacgagccg aagatcaccg ggcacccggg
    13201 acggatgagc tgcgccaggg cgatgccgga cagcgcctcg gccatctgct gcaccagggc
    13261 ggcggggatc gtcacgggag acatcgcacc catgaggatg aacggggtga ggaccacggg
    13321 ctgcgcggcg gcgcagtact cgaacagggc gtcgagcatg cggtcgtccc agcgcagggg
    13381 cgagttgcag ttgatcaggc tgatggaggc cgggcgggcc tcgatcgcct cgcggccgcc
    13441 gaagaggatc tcggacatcg cgatgacgtc gcgggcgttc tcgccggaga cgacgttgcc
    13501 catgaacacc ttgtcggtga gcgtcatgag cgacagcgtc atgtcgaggt ggcgcgagtc
    13561 caggggggcg tcattcggct cgcagaccac gccgcccgct gagtcgagca ccgagaagga
    13621 ctgggagagc ttcgcgaagt tgcggtagtc ctccagcgtg ccgtcgcgcc ggacgtcccc
    13681 ctcgcgcacg aagggcggac cgtagaccgc accgaaggcc atcgagtcgc cgccgatgtg
    13741 gatgctgcgc tcggcgttgc gggcgtgcac gtcgaactcg cgcggggcct tggcgacctg
    13801 ctcgagcacg aactcggggt cgaggaagac ggtgttgtcc tcgacccgct ggcccgcggc
    13861 ccggaacagg tccagggccc ggtcgctcat gaactcgacg ccgatctcgg tcacgagtcg
    13921 acgccacccg gcatccaact gggcgagggc atcggccgag aggatctcgt aacgcggcat
    13981 gcggttggtg aacatcgggc gtgcctttcg gtggcgatcg acattgacgg tgagccggct
    14041 cacatcctag cctcatcatg tgggactctg gttccacatc gtgaggcagt ttcttccggg
    14101 cgtgcccggc ccggagcagg agaggacgtc gcgtgaccac accaaccgtg ggtactggaa
    14161 cccagtcgat cgaccgtgcc gccgacctgc tcgcccgagt ggtccgcgca ccctcaccgg
    14221 tcaccttcac cgaactcgcc gaggtcaccg gcctcgcacg atcgacgacc tcccggctgc
    14281 tgtccgcgct cgagcgagcc gacctgctgg cgcgcgacga gcaggcacgc tggatcccgg
    14341 gtgccctctt cgaccactac gccgcccgac gttccgacga cgcacacctc gtcgaggccg
    14401 cggattccac gatgcgggcc ctgggggacc tgacgggcga gaccgtcaac ctcggtgtgc
    14461 cccgtcgcgg catggtcgtc caggtcgccc aggtcgactc ctcgtactac ctgggctcgc
    14521 gggactgggt cggcaccgac gtccccgccc actgctcagc cctgggcctg gtgctgtacg
    14581 cgcacggcgc cctgcccgcg ccctccggga agctcgaggc gctgactccc gcgtccctcg
    14641 cgaccggggc ggccctcaag gcgcgactcc ccggcatccg gcacgacggc ttctcgacca
    14701 ccgtcgacga gctcgaaccg gggctgaccg gggtggcggc accggtccgg ctcggtggca
    14761 cggtcgtcgc ggcgctcggc atctccgggc ccacctctcg tctcgcggcg gacctcaggt
    14821 ccaccggaac cctcgtcgcg gcacatgcca aggcgctctc gaaccgtctc gatcaccacc
    14881 accaggaagg cgcagcatga ctcccgacga aatcctccaa gggctctatg accagaccct
    14941 cgtcggcaac ggccctgccg tgctcgacct cacccaccag gcgctcgacg ggggcatgga
    15001 gcctggcacg atgctcttcg acgccctgat ccccgcgctg gaagaggtcg gtgcgcgctt
    15061 cgagcgcggc gacttcttcg tgcccgagat gctcatcgcc gggcgtgcga tggccgggtc
    15121 gatggaggtg ctgcggccgc tgctcgccga gaccggcgtc gagacgatcg gcaagttcct
    15181 catgggcacc gtcaaggggg acgtgcacga catcggcaag aacctcgtca acatcatgct
    15241 cgagggcgcc ggcttcgagg tcatcgacct cggcgtgcag gtggccccgg agaagttcgt
    15301 ctccgcgatc gaggagcacc agcccgacat cgtcggcttc tccgccttcc tcaccacgac
    15361 catgccgatg ttcaaggcga acatcaacgc cctccagaag gcggggctgc gcgactcggt
    15421 catcgtcatg gtcggcggcg ccccggtcac ccaggagtac gcggatgccg tcggtgccga
    15481 cggctacgcc tccgacgcct ccgcgaccgt caagcgcgcg aaggccctgc tcgaggcccg
    15541 tcggacgaag gtgcccgcat gagtgcgccc ctgcgccgga ccgtgctgcg ctcggccagc
    15601 aaggaggtcg tcatcgccgc cgaccagccg ttctgcgtca tcggcgagcg gatcaacccc
    15661 acgggccgcc cggtgttcca gaaggcgctg cgcgagggcg acctgtcgat catcgagaag
    15721 gacgtcgccg cccaggtggc cgggggcgcc aacgtcctcg acatcaacat gggggtgccc
    15781 ctcaccgacg aggccgacct gctcgtgcgg gccatccagc tcgtccagca ggtcaccgac
    15841 ctgccgatct gcatcgactc ctcggtcgtc gaagccctcg aggccgggct ggccgcctac
    15901 cagggccgtg ccctggtcaa ctcggtgacc gccgaggacg agcgactcga ggccatcctc
    15961 ccgctggtca agaagtacgg cgccgcgatc gtcgcgctgc ccaacgacga gcacgagatc
    16021 ccgatggagc accagcggcg cctcgagctc accgagaaga tcgtccgggt cgccacgcag
    16081 gagtacggga tcgccatcga cgacatcgtc atcgaccccc tcgccatgcc gatcggcgcc
    16141 gaccccaccg tgggtcgcga gacgctcctg acgatccgcg gcatcgtcga gcagtggggc
    16201 ctgaacacca cctgcggtgc ctcgaacgtc agcttcggga tgccgggtcg tcacgcgatc
    16261 aacggcgcct ggctctcgct cgccatgtcc gccggcatga ccagcgcgat catggacgcc
    16321 cgcacgccgc acgtcgtcga cgccgtgcgc gccagcgacc tgctgctcgg caacgacgag
    16381 tggggctcgg cgtggatcac cacgcaccgg gcgcgccagg ccgccgcagc cgccgaggcc
    16441 gcagccgctg aggcggccgc ggcatccacc gcctcggcgt gagcgacgga cgaggcccag
    16501 gagccccgtt cggcgacgtc gtcgacggtc tgcgccgcga gggcctgctc gaggccccgc
    16561 cgccggcggc ggaggtcggg gagccgcacg agggcaccgg ccgggtgcag ctgaccttcg
    16621 agccggccgg gcgcaccttc cgggtccccc cgggggtgtc ggtgttcgat gccgcctcgt
    16681 ggaacggcat cgccatcgac tccacgtgcg gagggcacgg cacctgccgc aagtgcctcg
    16741 ttcgggtgac ctccgggtcg gcgccggtga cccggcacga ccggcgcacc ttctccgagg
    16801 accagatcgc cgagggctgg cgcctggcgt gcctggtcaa ggcgacccac gacctcggcc
    16861 tcgaggtgcc accgctcgtc acgcgaccca aggcgtcgac ggtcggcgtc gggcgccagg
    16921 tcatcctgcg gccgggggtg cagaagcggt acgtcgagct gaccgacccg acgctggccg
    16981 accagcgcac cgacctgcag cgccttcgcg acgcggtcga cgacctcacc ctgaaccccg
    17041 acctgcacgc cctgcgccgg ctgcccaccg tgctgcgcca gagcgacttc aaggtcaccg
    17101 cggtcgtcgt cgacgaggtg ctcatcgacg tcgagcccgg tgacaccacc gggtcgcgcc
    17161 acgccatcgc gtacgacctc gggacgacga ctgtcgtcgc cacgctgctc gacctcgaga
    17221 ccggcacccc ggtggcggtg gcctcgatgc tcaacaagca acagcccttc ggcggtgacg
    17281 tgatcacccg gatcagcgcg acgatgatgg atccggaggc gctcgggcga ctcacccagc
    17341 tggcacgcca gaccctctcc gagctcgccg gggaggtgct cgacgagggt ggcatctcgc
    17401 cgcagagcgt gtacgaggtg gcgctggcgg gcaacgcgac gatgaccgcg ttgctgctcg
    17461 gcatcgaccc cgaaccgctg ggggtggcgc cgttcatcca ggtcagtgcc gactggccgg
    17521 tgctgcacgc cagcgacctc ggcatcgacg tgcaccccgg agcccgcgcg acgatcttcc
    17581 cggcgctcgg ggcctacgtc ggtggcgaca tcgtggcggg catgctcgcc agcggtctcg
    17641 accgcgacaa gcgggtgcgg ctgttcatcg acgtcggcac caactgcgag atcgccctca
    17701 gcgacggtga gcggatcgtc acgacggctg cccctgcagg gccggccttc gagggcggtg
    17761 ccatccgctg tggcatgcgg gcagccgacg gggccatcga ggtgatcacc ctgaacgcgg
    17821 gtgcgtccgg cgacgacgag gcggtcacac tcggcgtcat cggcgacgcg gaaccccgcg
    17881 gcatctgcgg ttcgggcctg gtcgatgccg tggccgagct cgtgcgcgtg ggcctgctcg
    17941 acgagtcggg tcgcctcatc agcgacgagg ccgccgccgc ctcggtgccg gcgctggccg
    18001 gtcggctggc caccatcggg gacggcgagc gggtgttcat cctgcaccgc ccgactcccg
    18061 atgcgccgac ggtcgagtgc gtctacctct cgcagcgcga cgtgcgcgag ctgcagttcg
    18121 ccaaggccgc gatctcgacg gggtggaccc tgctcctcga agagctcggc gtgtccccac
    18181 gggacgtgca gcaggtgctg ctcgccgggt cgttcggctc ctacctgtca ccgtcgtcgg
    18241 ccgtgcggat cggtctggtg cccaagctgc ccgtgctgcg catcgtctcg gcgggcaacg
    18301 tcgccggtga gggcgcgaag atggtgttgc tgtcggcgcg tgagcgggcc ggcgccgacg
    18361 cgatgctcgc tgacgtcgcg tacatcgagc tgtcggaccg gcctgacttc aacgaccgct
    18421 tcatcgacca gctcgcgttc cccgtctgag catgggcgtg ccgtctgggc gggtcgcgct
    18481 cgtcgcgtgc ggggccatcg cccaacccgc ggcggatgcc gtggcgcgcc gcgcgtggcc
    18541 cgtcgacgtc caccccctgc cgcccttgct ccacaaccgg cccgagcgca tcgcggatgc
    18601 cgtcgaggcc ctggtcctcc agctgcgggg gcgctacgac cgggtggtcg tgggctacgc
    18661 cgactgcggc acctacggag cgttggatgc cgtctgcgaa cggctgcacg tcgaccgcct
    18721 tccggggttg cactgttacg acctcttcgc cgggtcggac gtggtgagcc ggctctccga
    18781 ggacgagccg ggcacgtacc tgctcaccga cttcctcgtg aagtcgttcg cgcgcacggt
    18841 gatccaggaa ctcggcctcg accggcatcc cgaactgcgt gatgactact tccgccacta
    18901 cacccgggtc gtgtggctgg tgcagaccgc tgacccggtg gagcgggccg agctgcgcac
    18961 cctggcgcag gccgcggccg accgcatcgg gctgccgctc caggtg
//
LOCUS       DAOSOC010000029        18578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3004_length_18578_cov_10.397182, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000029 DAOSOC010000000
VERSION     DAOSOC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 18578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18578
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3004_length_18578_cov_10.397182"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /gene="rpsB"
                     /locus_tag="PL014_04050"
     CDS             complement(<1..325)
                     /gene="rpsB"
                     /locus_tag="PL014_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOT56294.1"
                     /translation="MAVVTMRQLLESGVHFGHQTRRWNPKMKRFIMTERNGIYIIDLQ
                     QSVTYINNAYEFVRETVAHGGTILFVGTKRQAQEPIAEQATRVGMPYVNHRWLGGMLT
                     NFQTIS"
     gene            complement(523..1929)
                     /locus_tag="PL014_04055"
     CDS             complement(523..1929)
                     /locus_tag="PL014_04055"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF009119.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT56295.1"
                     /translation="MDSQTLGTLDAVATAAAVREGRISARETVQAALARIEERNGPVN
                     AFTVVRAESALAEADELDAVGPSADRPLAGVPIVVKEEYDVTGDVTTLGGAGNSTPRT
                     ADSEVVRRLRAAGAVIVGRTTMPEFGQFPFTESLATGVTRNPFDLDRSPGGSSGGSAV
                     AVATGTVPVALGADGGGSIRIPASACGLVGLKPTRGRISVAPLSQHWYGLVVLGGMSR
                     SVRDSALLLDVISGSTPVDRWSVPPPREPFAAVAAREPGTLRIAWSTKCVTPGVDTDP
                     EITAATEQVARALVRLGHRVHRTAPRWPVATDAFLPQFYGGMAAEAGQVEHPERLEPR
                     TRQTARLARWATPRVVERAIRRGERVAAAVDARLLTDADVFLLPTMPKLPPPVGLLDG
                     MDAVRAQVATLPYVANTVLTNVSGHPALSLPAGLSATGLPIGVQLIARRGEEGLLLAV
                     AAQLEASRPDFWPGVATP"
     gene            1968..3122
                     /locus_tag="PL014_04060"
     CDS             1968..3122
                     /locus_tag="PL014_04060"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT56296.1"
                     /translation="MTRAPGNAALTPFGTTIFATMSALAVEHDAINLGQGFPDTDGPS
                     EVLEAAVAAIRGGANQYPPGAGVPELLDAIAAHQARFWGVSVDPRTEVLVTVGATEAI
                     AATVLALVAPGDEVVTFEPYYDSYAATIALAGGVRRTSVLRFPDFAVDESSLRAAFSS
                     RTRMVLLNTPHNPTGKVFTREEIELICELAREHDAWVVTDEVYEHLVFDGGQHVSVAS
                     LPGMAERTLTISSAGKTFSTTGWKVGWVSGPAAAVAAVRAVKQFLTYVGSGPFQPAVA
                     VGLGLPDSVFTGLARSLQGKRDRLVAGLRGAGLEVSVPAGGYFVVADAASLGAVDGLA
                     FCHELPVRSGVVGVPVSVFHDDPEAGRTLVRFAFCKRDEVLDEACRRLSS"
     gene            3240..3848
                     /locus_tag="PL014_04065"
     CDS             3240..3848
                     /locus_tag="PL014_04065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT56297.1"
                     /translation="MSLSSALAAMAGVLAAAVAASAPPDTRASLPGAQASGIHAVASA
                     VVGLLGAEVSGAGLGTPAAGERPRWQWPVEPRPEVARPFVAPLSPYGAGHRGLDLTAS
                     EGVAVLAVEGGVVTHAGVIAGRGTVSIEHADGLRSTYEPVTASVAGGDVVTAGQRIGT
                     LEATAGHCGPRGCLHLGARRGETYLDPLPLLAGGRIILLPFH"
     gene            complement(3849..4760)
                     /locus_tag="PL014_04070"
     CDS             complement(3849..4760)
                     /locus_tag="PL014_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOT56298.1"
                     /translation="MTVTSLEALPAFERHLRAERGRSVHTVRAYLRDVETFLSEAGVG
                     DDDGLREVSLADLRAWLGVLSRRGAARASIARTSASLRTFFGWLERTGRISTDPSLRL
                     TAPSRHRTLPPVLAQRSATALLDVAEVAADDDDPIHLRNRAALELLYATGIRVGELVG
                     LDVDDVDLASDVLRVVGKGDKERRVPFGVPARQAVAEWLTRGRPRLVGPQSGPALLLG
                     RRGRRADQRQIRSVVHDLLQHVPDAPDLGPHGLRHSAATHLLEGGADLRMVQELLGHA
                     SLATTQIYTHVSVERLKASYAQAHPRA"
     gene            complement(4867..6021)
                     /gene="dprA"
                     /locus_tag="PL014_04075"
     CDS             complement(4867..6021)
                     /gene="dprA"
                     /locus_tag="PL014_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT56299.1"
                     /translation="MSTSGVPGRRPSSLERDERWARVAWAFLAEPRETSVVAALGTFG
                     AVEALARFRAGHLLVRPGALERLPLLDVDDLAHAADRQGVHVVVPGDEGWPLGVDRLD
                     EPPYALFVRGYPDLDTLVERSVAVVGSRAATDYGMRTAADLAEGLGARGFTIISGAAY
                     GIDSAAHRAALASGGPTVAVLACGADRFYPAAHRSMLNEIARTGAVVSEVPVGAAPYR
                     SRFLARNRLIATLARATIVVEASQRSGSLTTARHARENHLPVGAVPGPVTSMASAGCH
                     ALIRDAEAVLVTDAAEVAELAGRIGEDVLPGFDELTEPRAPQDDLDPVSYAVWSAVPV
                     RSGASTEKIAAAAAVDARSLIGILAALEALGLVRRDAGTWRKVTGRRAAR"
     gene            complement(6059..7603)
                     /locus_tag="PL014_04080"
     CDS             complement(6059..7603)
                     /locus_tag="PL014_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOT56300.1"
                     /translation="MTFGLGLTRSVVLNGLDGVLVDVEAHIGQGLPYFAVGGTPDPAC
                     AQAPDRVKPAAATSGVPVPPHRITVNLSPASIPKRGAGFDLSIAVAVLSASGQIRGGR
                     ARDVVHLGELALNGRLRGVPGVLPAVLAAMRAGVRHVVVPLENVGEAQLVDGVVVHGA
                     ASLRDVIDWYTAAEEGVPLPPAPAPVPTVAPSCGGPDLADVVGQEEARLAIELSATGG
                     HHLLMSGPPGVGKTMLAERLVTVLPPLSREEALETHAIRSVTGQIGEVTRLDHTPPFV
                     APHHSATLAAIVGGGSGVALPGAISRAHHGVLFLDEAPEFKSSVLQTLRQPLESGEVI
                     IARARQMVRYPARFLLVLAANPCPCGRSYGKGADCTCKPMEIRTYVGRLSGPLLDRVD
                     LQVHVPPLQRAAIGDATGESSAVVAARVARARAAQAERWSGRGWATNGIVPGHVLRRA
                     PFRLPGAATAALDRSLDRGAVTLRGYDRVLRVAWSVADLLGRDSPGAADVAMALGLRS
                     GQLVAA"
     gene            complement(7600..7956)
                     /locus_tag="PL014_04085"
     CDS             complement(7600..7956)
                     /locus_tag="PL014_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOT56301.1"
                     /translation="MSRSTLGRVGEDLAVRWLVDHGMQVVERNWRCEHGEVDIVALDG
                     ACLVICEVKARRSLAFGEPVEAVTRAKAARLRRLAAVYARTHDTGAAHVRVDVLGILF
                     RANHPPLVRHVRGVGS"
     gene            complement(8191..8505)
                     /locus_tag="PL014_04090"
     CDS             complement(8191..8505)
                     /locus_tag="PL014_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HOT56302.1"
                     /translation="MSSEDLEKYESAAELALYREYRDVVGLFSHVVETERRFYLCNSV
                     DVKVRSDGGEVFFDVTMHDAWVWDIYRPARFAKTVRVVTFKDVNVEELAKSELELPKG
                     NF"
     gene            complement(8502..9290)
                     /locus_tag="PL014_04095"
     CDS             complement(8502..9290)
                     /locus_tag="PL014_04095"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT56303.1"
                     /translation="MRRTPSRRPSLRVERALQREGHRVLAGMDEVGRGALAGPVTVGV
                     VIIDESCGTAPQGVKDSKLLVPHARTAMVPRIERWAVAHGVGHASAAEIDDIGIIAAL
                     RVAGVRALAEAGIRPDLVILDGNHDWLTAPQDVGLFAFADDVPAATPPVSTMIKADLK
                     CSSVAAASVLAKVRRDAIMAGLGEQHPAYGWGENKGYAAPGHLDALRRLGPCELHRRS
                     WRLPGVMNDDGVVAADAAVPTDAPDVNDVDAMNAVDDLTRVGER"
     gene            complement(9287..10015)
                     /gene="lepB"
                     /locus_tag="PL014_04100"
     CDS             complement(9287..10015)
                     /gene="lepB"
                     /locus_tag="PL014_04100"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT56304.1"
                     /translation="MGSAVREVVVVIVMALLLSFVVKTWLLQAFFIPSGSMEDTLLVG
                     DRVVVSKLNPTPFAISRGDVVVFQDPGGWLDLPPPVERSGVSGAVHDALVFVGLLPAE
                     SEDHLIKRVIGLPGDRVTCCSDGGLLSVNGTPISEPYIKPGDVPSSLTFDITVPQGQV
                     WVMGDHRSDSEDSRFHDPSGNGSDGAVPIDAITGRAVAVVWPLGRAGWLSNYSSTFAP
                     VGEPTGALPDAPVGEPARTSAATP"
     gene            complement(10099..10650)
                     /locus_tag="PL014_04105"
     CDS             complement(10099..10650)
                     /locus_tag="PL014_04105"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOT56305.1"
                     /translation="MSARPQARPDADPAVAPVAAAGSRRHRPPFVLLLVAAVVLTALV
                     QGLLVQSYFVPSASLSPSLEPGDRALVWKVSPALDAGDVVVVDTTATASIDRATPVDD
                     GLAGRVLTPLANLLGVDTGRQDHLAVVGAADGDEVSLVAPVTGAVPASVSRDDIVGTA
                     VLRVWPLSRFGTIDTGTVAGVGS"
     gene            complement(10705..11064)
                     /gene="rplS"
                     /locus_tag="PL014_04110"
     CDS             complement(10705..11064)
                     /gene="rplS"
                     /locus_tag="PL014_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOT56306.1"
                     /translation="MQRFDELDSVSLKSDIPAFRAGDTVNVHVKVVEGTRSRIQVFKG
                     VVIRRHGGGIGETFTVRKVSFGVGVERTFPLHTPIIDHIEVVTRGDVRRAKLYYLRDL
                     RGKAAKIKEKRDTPVAR"
     gene            11249..12067
                     /locus_tag="PL014_04115"
     CDS             11249..12067
                     /locus_tag="PL014_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56307.1"
                     /translation="MKSADAVAYDRARLTAYGGRVTIDPGLPVALAVLLLLALTVTMY
                     RVGRLPSSGSTVVAAVRAIVQLSLAALVIAAVIRSLALSILLLIGMFTVAVVTTVRRV
                     EAPAAWPWATVAMLAGLLPVIAIILLTRTVPPTGAALVPVVGILAGNTMNGHTLTCRR
                     AFAALREEKGQYEAGLSLGLTRAQAIHEVIHRRVPEALIPGLDQVRTAGVVTLPGAFI
                     GVLLGGGSPAQAAAAQVLVLLGIMAAQTTTAVVAERLIGSARLLPADLRGSLQP"
     gene            complement(12071..13294)
                     /gene="trmD"
                     /locus_tag="PL014_04120"
     CDS             complement(12071..13294)
                     /gene="trmD"
                     /locus_tag="PL014_04120"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT56308.1"
                     /translation="MRLDVVTIFPGYLAPLDLSLIGKARRDGIVDVHVHDLRTFTDDR
                     HRTVDDSPLGGGAGMVMKPEPWARALDHVVDAGASEGAPVPHLIVPGPGGVPLTQALA
                     HQLAQEPWLAFACGRYEGIDERVYEHAGERMPVTIVSLGDYVLNGGEVAVLAIVEAVG
                     RLLPGVVGNAESLVEESHEDGLLEYPVYTRPAVWADGDVVREVPPVLLSGNHGAIAAW
                     RHEQRLERTAARRPDLLPVSATTGALADLDVRVATAADAPELRVLQLACWVPEGRASG
                     SWTIPPLDETLEEVTAGIGAWTTYAVRTASASGAPGRLVASVRGRVRPGDPTVWETGR
                     LMVAPDLQGRGLGRELLALAEAAAPASVTTFWINTGVVSKANQRRYQRAGYRQLPGEG
                     SFPGTVDLVKRRRTA"
     gene            complement(13300..13836)
                     /gene="rimM"
                     /locus_tag="PL014_04125"
     CDS             complement(13300..13836)
                     /gene="rimM"
                     /locus_tag="PL014_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591130.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOT56309.1"
                     /translation="MTERTVVARIGKPHGLHGEVTVQLHTDEPERRLAVGEALDTEAT
                     SGSGVPRVLTVRSTRVHNGIWLVAFEEIPDRTGAESLRGTRLVIDGDATLPAEDDEDV
                     FTEEQLRGLTVVDPAGTVLGQVGGLELGAAQDRLVVLLPDGREAQVPFVTAIVPTVDL
                     AGGKVVVDAPPGLLDLGV"
     gene            complement(13999..14241)
                     /locus_tag="PL014_04130"
     CDS             complement(13999..14241)
                     /locus_tag="PL014_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOT56310.1"
                     /translation="MLEEALEHLVTGIVDHKDQVQVRQKELRRGEILEVRVHPDDLGR
                     VIGRSGRTASALRTVMGALAGGRQVRIDIVDTDRVR"
     gene            complement(14244..14729)
                     /gene="rpsP"
                     /locus_tag="PL014_04135"
     CDS             complement(14244..14729)
                     /gene="rpsP"
                     /locus_tag="PL014_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT56311.1"
                     /translation="MAVKIRLKRMGKIRAPFYRVVVMDSRTKRDGRAIEEIGKYHPTE
                     EPSLIDIDSERAQYWLNNGAQPTEAVAALLKITGDWQKHKGLPGTEGTLKVKEAKTSK
                     RDLYEAALAAAGKSTAEDTSGATTAKKRAAKKVEEAPAAEAEATEAPAVEAEATDEAK
                     A"
     gene            complement(14938..15402)
                     /locus_tag="PL014_04140"
     CDS             complement(14938..15402)
                     /locus_tag="PL014_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT56312.1"
                     /translation="MDISARTEYAVRAMLALAQAHAAGEGPVSVDTLARQQDLPRKFL
                     EAIVGDLRVADLVVSTRGARGGYALARPASAISLGDVFRAVDGPLAEVRGLRPHETSY
                     EGVAQHLPTVWVAVRASLREVLDGTTLDQALTGALPPAVTSKAADPLAWQNR"
     gene            15565..16509
                     /locus_tag="PL014_04145"
     CDS             15565..16509
                     /locus_tag="PL014_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT56313.1"
                     /translation="MRKLVLLALVGLGAQLVDGSLGMAYGVTSTTLLLAIGTNPAAAS
                     ATVHLAEIGTTLASGASHWKFGNVDWKVVTRIGIPGAIGAFAGATFLSSLDTSVAAPV
                     MSLILLALGIYVLVRFTVKGLRRDRLGQPLRRRFLTPLGLVAGFVDATGGGGWGPVGT
                     PAILASGRLEPRKVIGSIDTSEFLVSVAASAGFLLALGSAGIDTAWVVALLVGGLIAA
                     PIAAWLVRHIPPRVLGSAVGGVIVLTNSRTLLRSDWIDASDSTRTLVYLVLAAVWAGA
                     VAWSVRAYRGELALERELADLEAELATDDARKGAAEPA"
     gene            complement(16607..17695)
                     /locus_tag="PL014_04150"
     CDS             complement(16607..17695)
                     /locus_tag="PL014_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT56314.1"
                     /translation="MASVLHLTGPVLLGPDDVRDQAWVLGGRITYEQPTGDHDVTTVP
                     GWVLPGLVDAHSHIGLDRHGATDAATAEQQALTDRSAGALLLRDAGSPADTRWVQDRE
                     DLPRLVRAGRHIARTRRYIRNYAWEVEPEQLVDQVRIEARSGDGWVKLVGDWIDRESG
                     DLAPCWPADVLAQAVAAAHEEGARVTAHCFGEESLRDLAAAGADCVEHACGLEPDTID
                     TFARQGIAIVPTLVNIDTFPQIAEPAREKFPDYHRHMIDLHARRFETIASAREAGIPV
                     YVGTDAGGSLPHGLVGDEVAHLGRAGFTPQQALDAACWGARAWLGWPGLGEGEEADLV
                     VYPADPRADLGVLAAPSLVVLRGIAHTP"
     gene            complement(17705..18529)
                     /locus_tag="PL014_04155"
     CDS             complement(17705..18529)
                     /locus_tag="PL014_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT56315.1"
                     /translation="MTAESLSTLAVGSAGPRVAFLHGLFGQGRNWTQIAKAIAGPDGS
                     AAQCLLVDLPDHGRSPWTQQFSYQTYAGRVAGTLSAAAPGEPWVLVGHSLGGKVAMVT
                     ALTNPELVRSLVVVDIAPKDYGDLDRFVHYIEAMRSLPLAELTGRADAEERFAQVEPD
                     AGVRGFLLQNLRRRGETWGWQANLDLIAADAALGQGSQIADFPETGAPPYEGPVVWLV
                     GGDSGYVRDEDTEQMRALFPRARPVVVKGAGHWVHSEAPEVVIETLRRVLRALPQH"
BASE COUNT         2543 a         6664 c         6767 g         2604 t
ORIGIN      
        1 tggagatcgt ctggaagttg gtgagcatgc caccgagcca gcggtggttg acgtacggca
       61 tgccgacgcg ggtggcctgc tcggcgatcg gctcctgcgc ctggcgcttg gtgccgacga
      121 acaggatcgt cccgccgtgg gccacggtct cgcggacgaa ctcgtacgcg ttgttgatgt
      181 aggtcaccga ctgctgcagg tcgatgatgt agatgccgtt gcgctcggtc atgatgaagc
      241 gcttcatctt ggggttccag cgacgggtct ggtgcccgaa gtggacgccg ctctcgagga
      301 gctggcgcat ggtgacgacg gccatgccgt gttctccttg tgcgttggtc agtttcgtcg
      361 gatgccgcgc gaaggtgcgg ggctcctgcc tggcgcctgc tacgcacctc accggatgcc
      421 gcggcatccg gactgccaag gtgcgccccg tcgtgtggtg gtccaggtga cggtgagaag
      481 gcgcgcgaag tcgcccgacc ggagtcggac gcacccgcca gtctacgggg tcgcgacccc
      541 cggccagaaa tcgggcctcg aggcctcgag ctgggcagcg acggcgagga gcaggccctc
      601 ctcgccgcgg cgggcgatga gctggacccc gatcggcagc ccggtggccg acagccccgc
      661 cggcaggctc agcgccgggt ggccggagac gttggtgagc acggtgttgg ccacgtaggg
      721 aagcgtcgcg acctgggcgc ggacggcatc catcccgtcc aggaggccga cgggcggggg
      781 cagcttcggc atcgtcggca ggaggaagac gtcggcgtcc gtgagcaggc gtgcgtcgac
      841 ggccgcggcg acccgctccc cccggcggat ggcgcgctcg accacccggg gcgtcgccca
      901 ccgggccagg cgcgcggtct gccgggtgcg gggttcgagc cgctcggggt gctcgacctg
      961 accggcctcg gcggccatgc caccgtagaa ctgcggcagg aacgcgtcgg tcgccaccgg
     1021 ccagcggggt gccgtgcggt gcacccggtg cccgagccgg accagcgccc tggcgacctg
     1081 ttcggtggcc gcggtgatct cggggtcggt gtcgaccccg ggcgtgacgc acttggtgct
     1141 ccacgcgatg cgcagggtgc ccggctcgcg agccgccacg gccgcgaagg gctcgcgcgg
     1201 cggcggcacc gaccagcggt cgacgggtgt cgagcccgag atgacgtcga gaagcagggc
     1261 gctgtcgcgc acgctgcgcg acatgccgcc gaggacgacg agcccgtacc agtgctgcga
     1321 caggggtgcc acggagatgc gcccccgggt gggcttgaga cccacgagcc cgcaggccga
     1381 ggcggggatg cggatcgacc cacccccgtc ggcgccgagc gccaccggca ccgtccccgt
     1441 cgcgactgcc acggccgacc cgccggacga gccacccggt gagcggtcga ggtcgaaggg
     1501 gttgcgggtg accccggtgg ccaggctctc ggtgaacggg aactggccga actcgggcat
     1561 cgtcgtgcgg ccgacgatga cggcaccggc ggcgcgcagg cgacgcacga cctcgctgtc
     1621 ggcggtgcgc ggcgtggagt tgccggcccc accgagagtc gtcacgtcgc cggtgacgtc
     1681 gtactcctcc ttgacgacga tgggaacccc ggcgaggggg cgatcggcac tggggccgac
     1741 ggcatccagc tcgtccgcct ccgcgagcgc gctctcggca cggaccacgg tgaaggcgtt
     1801 gacggggccg ttgcgctcct cgatgcgcgc cagggcggcc tgcaccgtct cgcgggcgct
     1861 gatgcggccc tcgcgcacgg cggcggcagt ggcgacggcg tcgagggtgc cgagggtctg
     1921 gctgtccatg cggtgcaggg tacgccgcac ggcatagcct ctcccccatg acccgagccc
     1981 ccggcaacgc ggcgctgacg ccgttcggta cgacgatctt cgcgaccatg tcggcgctgg
     2041 ccgtcgagca cgacgcgatc aacctggggc agggcttccc tgacaccgat ggtccgagcg
     2101 aggtgctcga ggcggccgtg gcggcgattc gcggcggggc caaccagtac ccgcccggtg
     2161 caggagtgcc cgagctgctc gatgccatcg ccgcacacca ggcgcgcttc tggggggtgt
     2221 cggtcgaccc gcgcaccgag gtgctcgtca ccgtgggggc gaccgaggcg atcgcggcga
     2281 cggtgctggc cctcgtggca ccaggtgacg aggtggtgac gttcgagccg tactacgaca
     2341 gctacgccgc gacgatcgcc ctggccggtg gggtgcgccg cacctcggtg ctgcgcttcc
     2401 cggacttcgc ggtcgacgag tcgtcgctgc gggcggcgtt ctcctcgcgg acccgcatgg
     2461 tgttgctcaa cacgccgcac aacccgacgg gcaaggtctt cacccgggag gagatcgagc
     2521 tgatctgcga gctggcgcgt gagcacgacg cgtgggtggt caccgacgag gtgtacgagc
     2581 acctcgtgtt cgacggtggg cagcacgtct ccgtggcatc gctgccgggg atggccgagc
     2641 gcacgctcac gatctcgtcg gccgggaaga cgttctcgac gacgggctgg aaggtcggct
     2701 gggtgagcgg tcctgctgcg gcggtggctg ccgttcgggc ggtcaagcag ttcctcacgt
     2761 acgtcgggtc ggggccgttc cagccggcgg tggcggtcgg gctggggttg ccggactcgg
     2821 tgttcaccgg gttggcgcgc tcgttgcagg gcaagcgcga ccggttggtc gccgggttgc
     2881 gtggtgccgg gctcgaggtg tcggtgcctg ccggcgggta cttcgtcgtc gcggatgccg
     2941 cgtcgctcgg ggccgtcgac gggttggcgt tctgccacga gctgccggtg cgctcgggcg
     3001 tggtcggggt gccggtgtcg gtgttccacg acgacccgga ggccgggcgc accctcgtgc
     3061 ggttcgcgtt ctgcaagcgt gacgaggtgc tcgacgaggc ctgccgccgc ctgtcgtcct
     3121 gaccccggcg gggcggcggt ggcggggtcg tcgtccacag gggtgccttt ggtcggatgc
     3181 cgtccacagg ctctcttggg cgactcgcct cgggtggggt ggctgggcac gctgggtgca
     3241 tgtccctttc ctcagccctg gccgcgatgg ctggtgtgct cgccgcagcg gttgccgcgt
     3301 ctgccccgcc tgacacgagg gcgagcctgc ctggcgcgca ggccagcggt atccacgccg
     3361 tggcctctgc cgtcgtcggg ctgctcgggg ctgaggtgtc gggtgctggg ctgggcaccc
     3421 cggcagctgg ggagcgtccg cggtggcagt ggccggtgga gccacgcccc gaggtggcgc
     3481 gcccgttcgt ggcgccgctc tcgccgtacg gtgcggggca ccgcggcctc gacctcacgg
     3541 catccgaggg tgtggcggtg ctggcggtcg agggtggcgt cgtgacgcat gccggggtga
     3601 tcgctggtcg ggggacggtc agcatcgagc acgcggacgg gttgcgcagc acctacgagc
     3661 cggtcactgc ttccgttgct ggtggtgacg tggtgacggc cggccagcgc atcggcaccc
     3721 tcgaagccac cgccggacac tgcggcccgc gaggctgcct ccacctgggc gcgcgccgcg
     3781 gcgaaaccta cctcgacccg cttccgctgc tcgcgggcgg ccggatcatc ctgctgccct
     3841 tccactgatc aggcgcgggg atgagcctgc gcgtacgagg ccttgagccg ctcgacggac
     3901 acgtgcgtgt agatctgcgt cgtcgcgagg gaggcgtgac cgagcagctc ctgcaccatg
     3961 cgcaggtcgg ctccgccctc gagcaggtgc gtcgcggcgc tgtgccgcag cccgtgtggg
     4021 ccgaggtcgg gtgcatccgg cacgtgctga agcaggtcgt gcaccacgga ccggatctgc
     4081 cgctggtcag cgcggcgccc tcggcgcccg agcagcaggg ccggcccgct ctgcggcccg
     4141 acgagccgcg ggcgaccgcg ggtgagccac tcggccacgg cctgacgagc cgggacgccg
     4201 aacggaacgc gacgctcctt gtcgcccttg ccgacgaccc gcaggacatc gctggccagg
     4261 tcgacgtcgt cgacgtcgag cccgacgagt tcaccgaccc ggatgccggt cgcgtagagc
     4321 agttcgagag cagcgcggtt gcgcaggtgg atcgggtcgt cgtcatcggc tgccacctcg
     4381 gcgacgtcga gcagggcggt ggccgaacgc tgggcgagaa ccggtggcag ggtccggtgc
     4441 ctgctcggcg cggtgaggcg cagcgagggg tcggtggaga tgcgaccggt gcgctcgagc
     4501 cagccgaaga acgtgcgcag cgacgcggag gtgcgggcga tgctggcgcg ggccgcgcct
     4561 cggcgcgaga gcactccgag ccacgcgcgc aggtcggcga gggacacctc gcgcagcccg
     4621 tcgtcatcgc ccacgcctgc ttcggacagg aacgtctcca cgtcgcgcag gtaggcccgc
     4681 acggtgtgca ccgagcgccc ccgctcggcg cgcaggtggc gctcgaacgc tggcagcgcc
     4741 tccaacgacg tgacggtcac ggcctcacgg tgtcacccca cccgtcacac ccgcaacacg
     4801 gcgcgcggca ccaggcgcgc ggtgtgcagc gccacccagc actcgacacc cagcaccttg
     4861 ccctcatcac cgagccgctc gtcgcccggt gaccttgcgc caggttccgg cgtcgcgccg
     4921 caccagcccg agcgcctcga gggcggcaag gatgccgatg aggctgcgcg cgtccaccgc
     4981 ggccgcagcg gcgatcttct ccgtcgatgc tcccgaccgc acaggaaccg ccgaccacac
     5041 ggcatacgac acggggtcga ggtcgtcctg cggcgcccgt ggctcggtga gctcgtcgaa
     5101 gccgggcagg acgtcttcac cgatacgccc tgcgagctcg gcgacctcgg ccgcatcggt
     5161 gacgagcacg gcttccgcat cacggatgag ggcgtggcag ccagcagacg ccatgctcgt
     5221 caccgggccg ggaaccgcac cgacgggcag gtggttctcg cgggcgtgtc tggccgtggt
     5281 caacgagccg gaccgttggc tggcctcgac gacgatcgtg gcgcgggcca aggtggcgat
     5341 gaggcggttg cgggcgagga agcggctgcg gtagggcgct gcgccgacgg gcacctcact
     5401 gacgaccgcc ccggtgcgag cgatctcgtt gagcatgctg cggtgggctg cggggtagaa
     5461 ccggtcggcc ccacaggcca ggacggcgac ggtgggccca ccggacgcga gggccgcgcg
     5521 atgggccgcc gagtcgatgc cgtaggccgc gccactgatg atcgtgaagc ctcgcgcgcc
     5581 gagcccctcg gccaggtctg cggccgtgcg catcccgtag tcggtggccg cccgcgaccc
     5641 cacgacggcc accgaccgct cgacgagggt gtcgaggtcg gggtagcccc gcacgaacaa
     5701 cgcgtacggc ggctcgtcga gccggtcgac accgagtggc cagccctcgt cgccgggcac
     5761 gacgacgtgg acgccctgtc gatcagcggc gtgcgcgagg tcgtcgacgt cgagcagtgg
     5821 tagtcgttcc aaggcgccgg ggcgaacgag caggtgcccg gcgcggaatc gggcgagggc
     5881 ctcgacggcg ccgaaggtgc ccagcgccgc gaccaccgag gtctcgcgcg gttcggccag
     5941 gaacgcccac gccacccggg cccaccgctc gtcacgctcc agcgaactcg gccgacgccc
     6001 gggcaccccg ctggtgctca tcggacgcac ccgtcgacag cgaacgcacg cggcgacgtc
     6061 atgccgccac cagctgacca ctgcgcagcc cgagtgccat cgcgacgtca gcagccccgg
     6121 ggctgtcccg accgagcagg tcggcaaccg accacgccac gcgcaggacg cggtcgtacc
     6181 cgcgcagcgt gaccgccccc cggtcgaggc tgcggtccag cgccgcggtc gctgccccgg
     6241 gcagccggaa gggcgcacgg cgcaacacgt ggccgggcac gatcccgttc gtggcccacc
     6301 cccgccccga ccaccgctcc gcctgggcag cccgggcccg ggccaccctc gctgcgacca
     6361 cggcactgct ctcccctgtc gcgtcgccga tggccgcgcg ctgcaacggc gggacgtgca
     6421 cctgcaggtc aacccggtcg agcaagggcc cggacaggcg acccacgtag gtgcggatct
     6481 ccatcggttt gcaggtgcaa tcggcaccct tgccgtagga acgaccacac gggcacgggt
     6541 tcgcggcgag gacgagcagg aaccgcgccg ggtaccgcac catctgtcgt gccctggcga
     6601 tgatgacctc gccggactcc agcggctggc gcaacgtctg gagcaccgaa ctcttgaact
     6661 cgggagcttc atccaggaac agcaccccgt gatgagcccg cgagatcgcc cccggaaggg
     6721 cgacgccgct tccaccaccg acgatcgcgg cgagggtcgc gctgtggtgg ggcgcgacga
     6781 acggcggggt gtggtcgagc ctggtgacct ccccgatctg gccggtcacc gagcggatgg
     6841 cgtgggtctc cagcgcctcc tcccggctga gtggcggcaa caccgtcacg agtcgctcag
     6901 cgagcatggt cttcccgacg ccgggtggcc cggacatcaa cagatgatgg ccgcccgtgg
     6961 ccgacagctc gatggccagc cgggcctcct cctgaccgac gacgtcagcg aggtcaggac
     7021 caccgcacga gggagccacc gttgggaccg gcgcaggtgc cggaggcagc ggcaccccct
     7081 cctccgcggc ggtgtaccag tcgatgacgt cccgcaggga tgccgccccg tgcacgacga
     7141 cgccgtcgac gagttgcgcc tcaccgacgt tctcgagcgg caccaccacg tgccggacac
     7201 ccgcccgcat cgccgcaagc accgcgggca ggacaccggg gaccccgcgc agccgcccgt
     7261 tgagcgccag ctcgccgagg tgcacgacat cgcgagcacg acccccacgg atctgccccg
     7321 acgccgagag caccgccacc gcgatcgaca ggtcgaagcc ggcgccacgc ttggggatgg
     7381 aggccggcga caggttcacc gtgatgcggt gcggcgggac cggcacccca ctcgtcgccg
     7441 cggcgggctt gacccggtcg ggcgcctggg cgcaggcggg gtccggggtg ccaccgacag
     7501 cgaagtacgg caggccctga ccgatgtggg cctcgacgtc gaccagcacc ccgtcgagcc
     7561 cgttcagcac cacggaacga gtcagcccca agccgaaggt catgacccca ccccccgcac
     7621 atggcgcacc aggggcgggt ggttcgcacg aaacaggatg ccgagcacgt caacccgcac
     7681 gtgggccgca ccggtgtcgt gggtgcgggc gtacaccgcg gccagtcggc gcaggcgcgc
     7741 cgccttggca cgggtcaccg cctcgaccgg ctcaccgaag gcgagggagc gacgagcctt
     7801 gacctcgcag atcaccaggc aggcgccgtc gagcgccacg atgtcgacct cgccgtgctc
     7861 acaccgccag ttccgctcga ccacctgcat cccgtgatcg acgagccagc gcaccgcgag
     7921 gtcctccccc acccgcccca gcgtgctcct cgacacgggt acgcccacca cgtcgaggcc
     7981 caccgactcc ggggtcggtg gctgatccgc ggggctcgcg gtgtctggtc gcccgggcgc
     8041 tcgttcgtga ctcgtcatga gcgcgagcca acccgccggc gggcggtgac gcgagcggca
     8101 tccgagcgcc tgtggacaat gccgcgcccc gacccgccct gtggacgatc acccccacaa
     8161 ccgcgcgggc agcggcgccg ccggtgggtg ctagaagttg cccttgggca gttcgagctc
     8221 gctcttggcg agctcctcga cgttgacgtc cttgaacgtc acgacccgca cggtcttggc
     8281 gaacctggcc ggtcggtaga tgtcccacac ccacgcgtcg tgcatcgtga cgtcgaagaa
     8341 gacctcgccg ccgtcgctgc gcaccttcac gtcgacgctg ttgcacaggt agaagcgccg
     8401 ctcggtctcg acgacgtggc tgaacagccc gacgacgtcg cggtactcgc ggtagagcgc
     8461 cagctcagcc gcgctctcgt acttctcgag gtcctccgag ctcatcgctc tcccactcgc
     8521 gtcaggtcgt ccacagcgtt cattgcgtcc acgtcgttca catcaggtgc gtcagtcggc
     8581 acggcggcat ccgccgccac cacgccatca tcgttcatca cgccgggcag ccgccacgag
     8641 cgccggtgca gctcgcacgg cccgagccgc cgcagcgcgt cgaggtgccc aggcgcggcg
     8701 taccccttgt tctcgcccca gccgtaggcg gggtgctgct cccccagccc ggccatgatc
     8761 gcgtcgcggc gcaccttggc cagcaccgag gccgcggcga ccgacgagca cttcaggtct
     8821 gccttgatca tcgtgctcac cggcggggtc gccgccggta cgtcgtcggc gaacgcgaac
     8881 agcccgacgt cctgcggagc cgtcaaccag tcgtggttgc cgtcgaggat gaccaggtca
     8941 gggcggatgc cggcctcggc cagggcgcgc acgcccgcca cccgcagcgc cgcgatgatc
     9001 ccgatgtcgt cgatctcggc ggcgctggcg tgaccgacgc cgtgcgccac cgcccagcgc
     9061 tcgatgcgcg gaaccatggc cgtgcgggcg tgggggacga gcagcttgga gtccttgacc
     9121 ccttggggtg ccgtgccgca ggactcgtcg atgatgacca cgcccaccgt gaccggcccg
     9181 gccaacgccc ctcgcccgac ctcgtccatc ccggcgagca cgcggtgtcc ctcgcgctgc
     9241 agggcgcgct cgacccgcag gctgggccgc cgcgacgggg tgcgcctcac ggtgtggccg
     9301 ccgaggtcct cgccggctca cccaccggcg cgtcaggcag cgcacctgtc ggctccccga
     9361 cgggagcaaa ggtcgacgag tagttcgaca accaccccgc ccggcccaac ggccacacca
     9421 cggcgaccgc gcgcccggtg atggcatcga tggggacggc gccgtcagaa ccgttgcccg
     9481 acgggtcgtg gaagcgtgag tcctcggagt cagagcggtg gtcgcccatg acccagacct
     9541 gcccctgcgg cacggtgatg tcgaaggtca ggctgctggg gacgtcaccg ggcttgatgt
     9601 acggctcgct gatgggcgta ccgttgacac tgagcaaccc accatcgctg cagcacgtga
     9661 cgcggtcacc cggcagaccg atgacccgct tgatgaggtg gtcctcggac tccgcgggca
     9721 acagcccgac gaacacgagg gcgtcgtgca cggcaccgga caccccggac cgctcgaccg
     9781 gtggaggcag gtcgagccac ccgccggggt cctggaagac gacgacgtca ccgcgcgaga
     9841 tcgcgaaggg ggtggggttc agcttggaga cgacgacccg gtccccgacg aggagggtgt
     9901 cctccatcga cccggagggg atgaagaacg cctggagcag ccaggtcttg acgacgaacg
     9961 agagcagcaa cgccatcacg atgacgacga cgacctcgcg gacagcgctg cccaccgccg
    10021 cgagcggccc ggaacggcgg tgcgagggtc cgtgaccggc atcctccgcg tcctcgggca
    10081 cgttgatgtc gggggcgctc acgaaccgac ccctgccacg gtccccgtgt cgatcgtgcc
    10141 gaaacggctg agcggccaca cccgcagcac ggccgtgccg acgatgtcgt cacggctcac
    10201 cgaggccgga acggcacccg tcaccggcgc caccagcgac acctcgtcgc cgtccgcagc
    10261 gccgaccacg gcgaggtggt cctgccgacc ggtgtcgacg ccgagcaggt tcgccagtgg
    10321 tgtcagcacc cggccggcga ggccgtcgtc gacgggcgtg gcgcggtcga tcgacgcggt
    10381 cgcggtggtg tcgacgacga cgacgtcacc ggcatccagt gccgggctga ccttccacac
    10441 gagggcgcga tctcctggtt ccaggctcgg cgacaggctg gccgagggga cgaagtagct
    10501 ctgcacgagc agcccctgca ccagcgcggt gagcacgacg gcagcgacga gcagcaggac
    10561 gaagggtggc cggtgacgac gagacccggc cgcggccacg ggggccactg ccgggtcggc
    10621 gtcaggacga gcctggggtc gtgcgctcac ccgcggagcc tagtcggcgg tgctgggtgc
    10681 ggcgcgcagg aggaccagat ctggtcagcg ggcgacgggg gtgtcgcgct tctccttgat
    10741 cttggcggcc ttgccgcgca ggtcgcgcag gtagtacagc ttggcgcggc ggacgtcacc
    10801 gcgggtcacg acctcgatgt ggtcgatgat cggggtgtgc agggggaagg tgcgctcgac
    10861 accgacgccg aaggacacct tgcgcacggt gaaggtctca ccgatgccgc cgccgtggcg
    10921 acggatgacg acgcccttga acacctggat gcgcgagcgc gtgccctcga cgaccttgac
    10981 gtggacgttg acggtgtcac cggcgcggaa ggccgggatg tcgctcttga gggagacgga
    11041 gtcgagctcg tcgaaacgct gcatgacgtg gtgtcgcttt cccacgggct gccacaggcc
    11101 atccgtgcat gatcggtact gcgtgatgtg atgggtgcga ccgcccggcc gcgtgctggt
    11161 cggaccgagg tccgggcact ccccctgtgg cgggggccga tggtgtcggc ggtgcagccg
    11221 cccagtctgc cagaggtgac cgttcgcggt gaaatcggcg gatgccgtgg cgtacgaccg
    11281 cgcccgcctc acggcatacg gtggtcgggt gacgatcgac cccggcctgc ccgtggccct
    11341 tgccgtgctg ctgctgctgg cgctgaccgt gacgatgtac cgcgtcggcc gcctgccgtc
    11401 gtcggggtcg acggtcgtgg cggcggtgcg tgccatcgtg cagctctcgc tcgccgcgct
    11461 cgtcatcgcc gcggtcatcc gctcgctcgc cctgtcgatc ctgctgctca tcgggatgtt
    11521 cacggtggcg gtcgtgacga ccgttcgccg ggtcgaggcg ccggcggcct ggccgtgggc
    11581 gaccgtggcg atgctcgccg ggctcctgcc ggtcatcgcg atcatcctgc tgacccgcac
    11641 ggtccccccg acaggtgccg ccctggtgcc ggtcgtcggc atcctcgccg ggaacacgat
    11701 gaacgggcac accctgacct gccggcgggc cttcgccgcc ctgcgcgagg agaagggcca
    11761 gtacgaggcc gggctctccc tcggcctcac ccgagcccag gcgatccacg aggtcatcca
    11821 ccgacgggtg cccgaggcgc tcatcccggg cctggaccag gtgcgcaccg ccggcgtcgt
    11881 caccctgccc ggggccttca tcggcgtgct cctcggcggc gggtcaccag cccaggccgc
    11941 ggcggcccag gtgctggtgc tgctcggcat catggctgcg cagacgacca cggccgtcgt
    12001 cgccgagcga ctcatcggca gcgcccggct gctgcccgcc gaccttcgag gctcgctgca
    12061 gccctagccg ctacgccgtg cgacgacgct tgacgaggtc gaccgtgccc gggaaggacc
    12121 cctcaccggg cagctgccgg tagccggccc gctggtaccg acgctggttg gccttgctca
    12181 cgacgccggt gttgatccag aacgtcgtca ccgacgcggg tgcggcagcc tccgcgagcg
    12241 ccaacagctc gcgccccaga ccccgcccct gcaggtcggg cgcgaccatg aggcgcccgg
    12301 tctcccacac cgtcgggtca ccgggccgca cgcgcccacg caccgaggcg accaaccgcc
    12361 ccggggcacc cgacgcggat gccgtgcgca cggcatacgt cgtccacgca ccgatgcccg
    12421 cggtcacctc ctcgagcgtc tcgtcgagcg gcgggatggt ccagcttccg gacgcgcgac
    12481 cctcgggcac ccagcacgcg agctggagca cccgcagctc aggcgcgtcg gcggcggtgg
    12541 cgacccgcac gtcgaggtcg gccagggccc cggtcgtcgc cgacaccggc aggaggtcgg
    12601 ggcggcgggc ggcagtgcgc tccaggcgct gctcgtggcg ccaggccgcg atggcgccgt
    12661 ggttgccgga gagcagcacc ggcggcacct cgcgcaccac gtcaccgtcg gcccacacgg
    12721 ccggcctggt gtagacgggg tactcgagca gcccgtcctc gtgggactcc tcgacgagtg
    12781 actcggcgtt gccgacgacc cccggcagca gccggccgac cgcctcgacg atcgccagca
    12841 ccgcgacctc tcccccgttg aggacgtagt ccccgagcga gacgatcgtg accggcatcc
    12901 gctcgccggc gtgctcgtag acgcgctcgt cgatgccctc gtagcgaccg cacgcgaagg
    12961 ccagccacgg ctcctgcgcg agctggtggg ccagcgcctg ggtgagcggc accccacccg
    13021 ggccggggac gatgaggtgc ggcaccggtg caccctcgct cgccccggca tccacgacgt
    13081 ggtccagcgc gcgcgcccag ggctcgggtt tcatgaccat gcccgcgccc ccaccgagcg
    13141 ggctgtcgtc gacggtgcgg tgccggtcgt cggtgaacgt gcgcaggtcg tgcacgtgga
    13201 cgtcgacgat cccgtcgcgc cgcgccttgc cgatgagcga gaggtcgagc ggcgcgaggt
    13261 agccagggaa gatcgtgacg acgtcgaggc gcatcgcggt cagaccccga ggtcgagcag
    13321 cccgggcggg gcatcgacga cgaccttgcc ccccgcgagg tcgaccgtcg ggacgatggc
    13381 cgtgacgaac ggcacctgcg cctcccggcc gtcggggagc aggacgacga gccggtcctg
    13441 ggcggcgccg agctcgagcc cgccgacctg cccgagcacc gtgcctgcgg ggtccaccac
    13501 ggtgaggccg cgcagctgct cctcggtgaa cacgtcctcg tcgtcctcgg cgggcagggt
    13561 tgcgtcaccg tcgatgacga ggcgggtgcc acgcagcgac tcggcgccgg tgcggtcggg
    13621 gatctcctcg aacgccacga gccagatgcc gttgtgcacc cgggtggagc gcacggtgag
    13681 cacgcgcgga acgccggagc cactcgtggc ctcggtgtcg agggcctctc cgacggcgag
    13741 ccgacgctcc ggctcgtcag tgtgcagctg gacggtcacc tcaccgtgca ggccgtgggg
    13801 tttgccgatg cgggcgacga cggtgcgctc ggtcacgggg gccatcctgt cgtggggtgc
    13861 tgggctgcac catgtggtgc gcggggtgag tcggggtggg gcgggacatt gcggcggcgg
    13921 ggtgccccgg ggagtttcct cccgggacac cccgccgtcg tggtggtcgc tctcggcgac
    13981 cgacgtcagc gactcgggtc agcggacccg gtcggtgtcg acgatgtcga tccgcacctg
    14041 gcgcccgccg gcgagcgcac ccatgacggt gcgcagtgcg ctggcggtgc ggcccgagcg
    14101 gccgatgacc cggccgaggt cgtcggggtg cacgcgcacc tcgaggatct cgccgcggcg
    14161 cagttccttc tggcggacct gcacctggtc cttgtggtcg acgatgcccg tgacgaggtg
    14221 ctcgagcgcc tcttcgagca cggtcaggcc ttcgcctcgt cggtggcctc ggcctcgaca
    14281 gccggagcct cggttgcctc ggcctcggcg gccggggcct cctcgacctt cttggcggcg
    14341 cgcttcttgg cggtggtcgc accggaggtg tcctcggcgg tggacttgcc ggcggcagcc
    14401 agggctgctt cgtacaggtc gcgcttggag gtcttggcct ccttgacctt cagggtgccc
    14461 tcggtgcccg gcaggccctt gtgcttctgc cagtcaccgg tgatcttcag cagggcggcg
    14521 acggcctcgg tcggctgggc gccgttgttg agccagtact gcgcgcgctc gctgtcgatg
    14581 tcgatgagcg agggctcctc ggtggggtgg tacttgccga tctcctcgat cgcccggcca
    14641 tcgcgcttgg tgcgcgagtc catgacgacg acacggtaga acggtgcacg gatcttgccc
    14701 atgcgcttga gacgaatctt gacggccacg gtggtggtca ctcctggtac gtgatgagcg
    14761 cggcaagacc gcgcacggat cgcgcgggga gccgggagct cggtgtaggc gtccggtccg
    14821 gcgatgcgca gacggcagcg accggacggg tacagccgtc cattctgcca gatgccgtgc
    14881 ccggtcccga ccacgccggg gtggggcgtc ggcccgggcg ccaggcccgg gcggagctca
    14941 ccggttctgc cacgccaagg ggtcggcagc cttcgaggtc accgcgggcg gcagggcgcc
    15001 cgtgagggcc tggtcgagcg tggtgccgtc gagcacctcg cgcaggctgg cccgcaccgc
    15061 cacccacacc gtcggcaggt gctgcgccac tccctcgtag ctcgtctcgt gagggcgcag
    15121 gccacgcact tcggcgagcg gaccgtcgac ggcacggaag acgtcaccga gggagatcgc
    15181 ggatgccgga cgcgcgagcg cgtacccgcc gcgggctccg cgggtgctca ccacgaggtc
    15241 ggccacgcgc aggtcaccaa cgatcgcctc gaggaacttg cgcggaagat cctgctgacg
    15301 ggcgagcgtg tcgacactga ccgggccctc accggccgcg tgggcctgcg cgagcgcgag
    15361 catcgcccga acggcgtact cggtgcgagc ggagatgtcc acgtcggcat cctcccccac
    15421 cgatgagggc gaggcgccgt cgcgggcggc gagcacccgg catccaccat gcgagacata
    15481 acttttcatt ggttgaccca taacaagacg ttggataaaa ctcctacttg tccggtcgga
    15541 ttaccactcc ctggagcctg tcccgtgcga aagctcgttc tcctcgccct cgtcggcctc
    15601 ggcgcccaac tcgtcgacgg gtcgctcggc atggcgtacg gggtgacgtc gacgacgctc
    15661 ctgctcgcca tcggcaccaa cccggcggcg gcctcggcga ccgtgcacct cgccgagatc
    15721 ggcacgacgc tggcctcggg tgcgtcccac tggaagttcg ggaacgtcga ctggaaggtc
    15781 gtcacccgca tcggcatccc cggcgcgatc ggagccttcg ccggcgcgac cttcctgtcc
    15841 agcctcgaca cctcggtcgc cgcccccgtc atgtccctga tcctgctggc gctgggcatc
    15901 tacgtgctcg tgcgcttcac cgtcaagggc ctgcggcggg acaggctcgg ccaaccgctg
    15961 cgtcggcgct tcctcacccc gctcggtctc gtcgccggct tcgtcgacgc gaccggtggc
    16021 ggtgggtggg gccctgtcgg gacgccggcc atccttgcga gcgggcgcct ggagccccgc
    16081 aaggtcatcg gctcgatcga caccagcgag ttcctcgtct cggtggccgc gagcgccggc
    16141 ttcctcctcg cgctcggatc cgccggcatc gacaccgcat gggtggtcgc gctgctcgtc
    16201 ggcgggctca tcgccgcccc gatcgccgcg tggctggtgc ggcacatccc gccccgggtg
    16261 ctcggctcag cggtgggcgg ggtcatcgtg ctcaccaaca gccgcaccct gctgcgcagc
    16321 gactggatcg acgcgtcgga ctccacccgc accctcgtct acctcgtgct ggccgcggtc
    16381 tgggcgggcg cggtcgcctg gtcggtgcgg gcctaccggg gcgaactcgc tctcgagcgt
    16441 gagctcgccg acctcgaggc agagctcgcc acggacgacg cccgcaaggg cgcggccgaa
    16501 cccgcctgag cacgccctca ccacaccccc tgaacccgac ttgttgcacg tgagccaccc
    16561 ggatccgggt ggctcacgtg caatgagtcg ggcagatgag ggcgggtcag ggggtgtggg
    16621 cgatcccgcg cagcaccacc agcgacgggg cagcgagcac tccgaggtca gcccgcgggt
    16681 cggccgggta cacgacgagg tcggcttcct ccccctcccc cagcccaggc catcccagcc
    16741 acgcccgcgc accccagcac gcggcatcca acgcctgctg cggcgtgaag ccggcccgcc
    16801 cgaggtgcgc gacctcgtcg ccgacgagtc cgtggggaag tgagcccccg gcatccgtgc
    16861 cgacgtagac cgggatgccg gcctcgcgcg ccgacgcgat cgtctcgaac cggcgcgcgt
    16921 gcaggtcgat catgtgccgg tggtagtcgg ggaacttctc gcgagccggc tccgcgatct
    16981 gcgggaaggt gtcgatgttg accagcgtgg gcacgatcgc gatgccctga cgggcgaacg
    17041 tgtcgatggt gtcgggttcg agcccgcagg cgtgctcgac gcagtcggca cctgccgcgg
    17101 ccaggtcacg cagtgactct tcgccgaagc agtgcgccgt gacccgggcc ccctcctcgt
    17161 gggccgccgc gaccgcctgc gccagcacgt cagccggcca gcacggcgcg aggtccccgc
    17221 tctcccggtc gatccagtcg ccgacgagct tgacccaccc gtcgcccgag cgcgcctcga
    17281 tgcgcacctg gtcgacgagc tgctcgggct cgacctccca cgcgtagttg cggatgtagc
    17341 ggcgcgttcg ggcgatgtgc cgcccggccc gcacgagccg tggcaggtcc tcgcggtcct
    17401 gcacccaccg ggtgtcggcg ggcgagccgg catcgcgcag gagcagggcc ccggcggatc
    17461 ggtcggtcag ggcctgctgc tcggcggtgg ccgcgtcggt cgccccgtgg cggtcgagcc
    17521 cgatgtggct gtgggcgtcg accaggcccg gcagcaccca gccgggcacg gtggtgacgt
    17581 cgtggtcccc ggtcggctgc tcgtaggtga tgcggccgcc gagcacccag gcctgatctc
    17641 ggacgtcgtc ggggccgagc aggacagggc cggtgaggtg caggaccgaa gccatgcccc
    17701 gaccctagtg ctggggtagc gccctcagca cgcggcgcaa cgtctcgatg acgacctcag
    17761 gcgcctcgga gtgcacccag tgccccgccc ccttgacgac caccggccgc gcacgcggga
    17821 acagcgcccg catctgctcg gtgtcctcgt cacgcacgta gccggagtcg ccgccgacga
    17881 gccacaccac cggtccctcg tacggcgggg cgccggtctc ggggaagtcg gcgatctgcg
    17941 agccctgacc gagcgccgcg tccgccgcga tgaggtcgag gttggcctgc cagccccagg
    18001 tctcaccccg acgccgaagg ttctgcagca ggaacccgcg caccccggca tccggctcca
    18061 cctgcgcgaa gcgctcctcc gcgtcggcgc gcccggtgag ctcggcgagc ggcagcgaac
    18121 gcatcgcctc gatgtagtgc acgaagcggt cgaggtcgcc gtagtccttg ggtgcgatgt
    18181 cgacgacgac gaggctgcgc acgagctcgg ggttggtcag ggccgtgacc atggcgacct
    18241 tgccgccgag cgagtggccg acgagcaccc acggctcccc cggcgctgcg gccgacagcg
    18301 tgccggcgac gcgcccggca tacgtctggt acgagaactg ctgcgtccac ggcgagcgtc
    18361 cgtggtcggg aaggtcaacg agcagacact gggcggcgga accgtcaggg ccggcgatcg
    18421 ccttggcgat ctgggtccag ttccggccct gcccgaacag tccgtggaga aaggccaccc
    18481 gcgggccggc gctgccgacc gcgagcgtcg agagcgactc tgcggtcact gccccaggaa
    18541 cttctcgaag cccttgggca ggcttgccgg gtcgatgg
//
LOCUS       DAOSOC010000030        18433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3032_length_18433_cov_9.259767, whole genome shotgun sequence.
ACCESSION   DAOSOC010000030 DAOSOC010000000
VERSION     DAOSOC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 18433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18433
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3032_length_18433_cov_9.259767"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PL014_04160"
     CDS             <1..342
                     /locus_tag="PL014_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT56316.1"
                     /translation="GLKGVDASLREAAAVDGAGEVKTFFHVILPVMRPINLIVLVIVV
                     IESLRAFDLVWIINKGRNGLELIGALVAQNVVGEATRFGFGSALAVIMMLISTVFIVI
                     YLRIVFREERR"
     gene            339..1265
                     /locus_tag="PL014_04165"
     CDS             339..1265
                     /locus_tag="PL014_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT56317.1"
                     /translation="MSATTTSPSTTGAAKPSVHTGAGGVMNKRRGTIATVVMLFLALL
                     WLFPLLWALYNSFRSYSYTQTNGYVSFGGWTLDNYQNAWSRGNFGLHLKNSLLITIPA
                     VALTLFLSSMVAFVLARFSYRFNLTLLGVFLAANLLPPQALLIPVFKMFREIPLPTFM
                     SESGTMLNSFWALILVNTAFQMGFCTFVLSNYMKTLPYEIYESAQLDGASVWRQYWQL
                     TMPLVRPALAALATLQVTWIYNEFFWATVLISRGDKLPVTSALNNLRGQFFADTNLVA
                     AGSIIVALPVLIVFFVLQKQFVAGLTLGSTKG"
     gene            1262..3424
                     /locus_tag="PL014_04170"
     CDS             1262..3424
                     /locus_tag="PL014_04170"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT56318.1"
                     /translation="MSTPLVIVRGAGSALVLTQDEGGALPHVVHWGTDLPDAVLGDLA
                     HLTGAPVPHNQPDAPWHVSVLPTSGQGWLGTPALEVHRAGGRTAPRWVATLEGGDHDA
                     TVRATASDIGLDVVVRYRLDEHGVLSVETELTNTAAEGEPVDVGALRAVLPLPTRADE
                     VLDLTGRWCRERAPQRRPLTDGTWVRTGRRGRTGHDATLLMAAGTTGFGFRSGEVWAT
                     HVAWSGNHEHLVERLPEGAGAHAAVLGGGEALTPGEVRLAPGETYRAPSVVFVHSTEG
                     LDGITRALVRHQRAQPGYRTTARPLVLNTWEAVYFQTDLDHLRRLADTAAAIGVERFV
                     LDDGWFGSRRDDHRGLGDWVVSHEVWPDGLSPLVDHVKGLGMEFGLWFEPEMVNLDSD
                     LVRANPHWVLGPVAGAPLPSRHQHVLDLANPDAFAHVLEAMSSLVAEYGIDFIKWDHN
                     RDLHEVVRTDGSGTDRPAVHAQTSATYRLVDELRRRHPGLEIESCSSGGARVDLGVLE
                     RTDRVWTSDCNDALERVAIQRWTGLLVPPERMGTHIGPPTAHTTHRHLDLSLRMLVAL
                     GGHAGLEWDITTCSADELDALRAWSALYRELRPLLHGGDAVRSDHPDPALVVGGVVAP
                     DRSAAVFSVVQAVSGRGVAPGVVPLPGLDAGRRYRVRVRSEAGLPAVIQVHPPAWWDA
                     ALGDGIVVSGAALGEVGLALPVLAPAQGFLLHLEGVDA"
     gene            3452..3877
                     /locus_tag="PL014_04175"
     CDS             3452..3877
                     /locus_tag="PL014_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HOT56319.1"
                     /translation="MAIPPSHLSFAALEFMTERHLATLTTLRADGSPHVVPVGFTWDD
                     DLLVARVIASRSSAKVVQARAGRRAALSQVQARRWITLEGSTRVLEDPASVRDAEQRY
                     AQRYRVPRENPERVVIVLDVDRVLGGDWPHPLPPSPYAT"
     gene            4014..4229
                     /locus_tag="PL014_04180"
     CDS             4014..4229
                     /locus_tag="PL014_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56320.1"
                     /translation="MKRFLVAGAVAAFAMVGFSSTAQAAPAQAACFGQVHKLVNSGGL
                     PGIDNVGQLVGALGGGQNKNATAKTFC"
     gene            complement(4333..5013)
                     /locus_tag="PL014_04185"
     CDS             complement(4333..5013)
                     /locus_tag="PL014_04185"
                     /inference="COORDINATES: protein motif:HMM:NF020119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HOT56321.1"
                     /translation="MGRHAAPLVRPRADAGGQRHPERACRRRRGRPRPLGPVGRLAQQ
                     GLPVRISGTPTVDLEVTQGRPVGQLSVQLVDYGAMDRVLTTNDGALTLTTESCWGAST
                     AVDDACYRDVTRRVGPTELQVLARGWIRLDGEGTKNVTVELQANDVVVPAGHQLGLVI
                     SGNRSGVTAIDTSAQTYSVNLATSRLNLPIEGPMASFAPGQIKPEKTADLREGTLPLL
                     DEFIVPQP"
     gene            complement(4932..5837)
                     /locus_tag="PL014_04190"
                     /pseudo
     CDS             complement(4932..5837)
                     /locus_tag="PL014_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
     assembly_gap    5962..6061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6062..6628)
                     /locus_tag="PL014_04195"
     CDS             complement(<6062..6628)
                     /locus_tag="PL014_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT56322.1"
                     /translation="MSIRVLVADDQPIVRSGLTMLLDAQPDIDVVGAAADGREAVRLA
                     RQLRPDVGLFDVRMPVMDGIEATRQLAGPDVEDPLPIIVITTFDLDEYVHGALKAGAR
                     GFLLKDAGPDLLTRAIHAAAEGDALIAPSVTVRLLATFATSRAEAARQPLEPLTAREE
                     EVLVTVARGRTNDEIASELFITTSTVKAH"
     gene            complement(6625..7773)
                     /locus_tag="PL014_04200"
     CDS             complement(6625..7773)
                     /locus_tag="PL014_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT56323.1"
                     /translation="MATDALRSLWVEPRPTNPPATSAADWALVAAFVGWAVNEAVLRD
                     GMAPAPVLLIATLAAVAPLPWRRSHPLPAVLVAFGTLIVVDLFRMATGTQGALTSSVS
                     ATLVLTYALFRWGSGRDAVRGLLVILTWLAITFVADVTTLTDTITGYAFFFFAAALGA
                     AVRYRARIRIRDIQEAKAREREQLARELHDVVAHHVSGIAIQAQAGRAVAATHPERAV
                     DALATIEEAATRALTELRAIVGVLRDARHSSLTPQPGMADIEQLATTGAAQPRVEVTL
                     TGELTDLSPSVAAAVYRLAQESVTNSRRHARHATRVTVQVTGETDHVRLTVDDDGSTG
                     SPHRSPDGYGLVGMRERVTLLGGTFAAGPTADRGWRVEANLPRAGVAR"
     gene            7943..8443
                     /locus_tag="PL014_04205"
     CDS             7943..8443
                     /locus_tag="PL014_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HOT56324.1"
                     /translation="MSTSRPDTALDDVRLPLKARLAAAWTSFMFLYVYVDHLSLYKPG
                     VLDELLAGRVHEFDTGPTFIGAGLTLVAVPSLMIVLSMTLPARVNRVVNLVVAALYLP
                     VTVYNVAGEPMSYAYFYGLSIGLEVLLLAYIAWSAWAWPRVAAPAAGAAGAGLPTGKS
                     ANSPVG"
     gene            8579..9196
                     /locus_tag="PL014_04210"
     CDS             8579..9196
                     /locus_tag="PL014_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56325.1"
                     /translation="MTTFAGDGGLFVSDLLVIGLLVLLAVVLGFGLTVTFIVWRLVRG
                     VRRWVARRRGVAAAQPRMVRKPGPARDVAKLRRELSDEVSAAELMLSRAKNGRVFVAD
                     AQTLLDDLKVGAASLDANMASVQNYSDAAQQRAALEALRPQVAKLIETSYLARQTLLQ
                     TEIADRERHLTDLSDHVERQAEALKIYRDESHSLNLDDLREHPAR"
     gene            9467..11095
                     /gene="purH"
                     /locus_tag="PL014_04215"
     CDS             9467..11095
                     /gene="purH"
                     /locus_tag="PL014_04215"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT56326.1"
                     /translation="MAVEVSTPGHTQDVRPVRRALISVFDKAGLDDLVRGLHEAGVAL
                     VSTGGSAALIESLGLPVTKVEELTGFPECLEGRVKTLHPLVHAGILADTRKPDHVAQL
                     AELGVEAFDLVVVNLYPFADTVASGAAADQIVENIDIGGPSMVRAAAKNHPSVAVVTD
                     PSTYGDVLDAVRGGGFTFAQRQRLAAQAFVHTATYDVAVASWMGNAYTDTSEGTGFPA
                     WAGATWDKAAVLRYGENPHQRAALYTNGFQPQPGLAQATQLHGKEMSYNNYIDADAAV
                     RAAYDHGDQPTVAVIKHANPCGIAVGTDIADAHRKAHECDPVSAFGGIIATNRPVTVE
                     MARTVADIFTEVVVAPGFDDDALEVLTAKKNVRLLRCAAPGRGGVETRPISGGLLMQQ
                     RDGVDAVVRGDDDTVSGGDDVVNWRLVSGDPADEATLADLQFAWRAVRAVKSNAILLA
                     RGGGSVGIGMGQVNRVDSCHLAVSRAGEDRARGAVAASDAFFPFADGLQVLLDAGVRA
                     VVAPGGSMRDAEVIEAAQAAGVTMYFTGTRHFAH"
     gene            complement(11159..12226)
                     /locus_tag="PL014_04220"
     CDS             complement(11159..12226)
                     /locus_tag="PL014_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HOT56327.1"
                     /translation="MRVSVTIPAAGHHGRRTRLDGLDAARGLAVVSMLVAHLSPVGGP
                     ATITEYLTAPLFAVIIGTSMGIHLRDRSPHPDRFLGDNLQRGLILILLGILLQRLHDQ
                     VIIVLPHLGVLVIVLAPLALLLRRMPVLTVGLAVGLAVVAPVVVDRARLAANENIATW
                     PGWLDDLMGWVATGDAYRLATLLPMALGGLALAAVLHQVTHLPQAWVVAGALFATSLA
                     AYRLGAGTASGSAAYSGTTAEVIGATLLATGSVVASFIMLALAERFSLRRVLEPVLAT
                     GRLALTAYTVQILWLALLSVLRDGAPDDAWWILGSSLVVVVGACWALDRWWGTGPLEW
                     LVHRLRPRRVPDTTPRHAATR"
     gene            complement(12312..13451)
                     /locus_tag="PL014_04225"
     CDS             complement(12312..13451)
                     /locus_tag="PL014_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HOT56328.1"
                     /translation="MPDAIEVRKVPLHTVSDASELGKLIDEGVFEASRVIAVIGKTEG
                     NGGVNDYTRIIADRAFREILVEKGADPAAVKEIPIVWSGGTDGVISPHATIFATVPAD
                     KAEQTDEPRLTVGFAMSEHLLPEEIGYTSMVSKVADAVKVAMERAGITDPADVHYVQT
                     KTPLLTIHTIRDAKARGKQVWTEHTHESMDLSNGCTALGIAVALGEIEMPTDADVMHN
                     RELFSSVASCSSGVELDQAQVVVVGNARGVGGRYRIGHSVMNDALDQDGIWAAIKDAG
                     LDLPERPHRSDLEGRLVNVFLKCEASLDGQVAGRRNAMLDDSDVHWHRQIKSCVGGVT
                     ASVTGDPAVFVSVSAAHQGPEGGGPVAAIVDLGSGEPTGYRPPTT"
     gene            complement(13512..14447)
                     /gene="arcC"
                     /locus_tag="PL014_04230"
     CDS             complement(13512..14447)
                     /gene="arcC"
                     /locus_tag="PL014_04230"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOT56329.1"
                     /translation="MRVVLALGGNAMTAPDGSATPAAQIAAVEVAMTAAARLVAAGHE
                     LVITHGNGPQVGNLLVKNELAAHVVPPVPLDWCGAQTQGTLGFTILDALDDALAATGT
                     RRPVAAIVTRTLVDRDDPGFTHPTKPIGRYLSHAQAQPMIDHGQVWQDRGEKGWRRVV
                     ASPEPLEVLDVPALLTLLEAGHVVVAAGGGGIPVVRDPAGRLRGVEAVIDKDLTAALL
                     ARTIDADVLVIATDVDHAAVDWGTPTQRDLGRVTVSQLRAHAADGQFASGSMGPKVDA
                     AIRFVAGGGPRSVITSLTHITDAVEGSFGTVVDPD"
     gene            14663..15604
                     /locus_tag="PL014_04235"
     CDS             14663..15604
                     /locus_tag="PL014_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT56330.1"
                     /translation="MTARRAADRTWLVALAASLWGLSALWRGPLAKEYPALAIVFWEH
                     LVLVVLVSPWIVPAVRRVLAASTRTKVSVLVIGAGSSALATTLFTAAFRLGDPITPQV
                     LQKLQPLIAIALAALVLGERLRRSYAVFALPAILGAWLLAFADPMGVSVSSAQAAALA
                     VGAAALWAAGTVLGRAASAELRFSDLTALRFTVGLVTLLAISGVTSTPLAIGVDAAPR
                     ILLLALFPGLLALVLYYRALGHTPASRATLAELAFPLTAAVVGIVAFDASLTGSQWVG
                     FALVLASVVGLALHEQRSSRPSVAVPDDAREALTAAR"
     gene            complement(15624..17117)
                     /locus_tag="PL014_04240"
     CDS             complement(15624..17117)
                     /locus_tag="PL014_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT56331.1"
                     /translation="MSERPRRPGAHELLAEVLDEGSFVSWDQAPLEVAPPGSTYAAEL
                     AAAAERAGTDESIITGEGRIHGHRVAVIVGEFTFLAGSIGRAAAERVTLAFERATRER
                     VPLFAAPASGGTRMQEGTPAFVTMVKISGAVAAFKAAQLPYITYLRHPTTGGVLASWG
                     SLGHVTVAEPGATIGFLGARVYEALYGEPFPADVQTAENLYEHGLLDAVLPVEALPPI
                     AGRFLDLVLAPHDTEPVPAVPREPLPDLPAWESVTRSRDPERPGVRALLKLGATDVLP
                     LFGTGAGEWDRSLFLALARFGGMPCVVLGQDRRAERAEAPLGPSGLRVARRGMKLATE
                     LGLPLVSVIDTAGAALSKEAEEGGMAGEIARCLAELVVLPVPTVCVLLGQGTGGGALA
                     LMPADRVVCAQHSWLSPLPPEGASAILHRTPDRAPELATQQRVRSLDLLEAGIVDRIV
                     AEHPDAAQEPEAYCRRMAQAIRHELGDLVALPPETRLQARHARWRAL"
     gene            complement(17114..18289)
                     /locus_tag="PL014_04245"
     CDS             complement(17114..18289)
                     /locus_tag="PL014_04245"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOT56332.1"
                     /translation="MAGPLDDVVVIDLTRALAGPHCAMMLGDMGARVIKVENPGGGDE
                     SRGWGPPFVGPDDEPVSTYFLSCNRNKESVTLDLKTDDGRETLARLVTHADVLLENFR
                     PGVLDRLGFSVERLHELNPGLVLLSISGFGHDGPQGGRAGYDQIAQGEGGLMSLTGAD
                     PEHPQRVGVPIGDLLAGMYGAYGVVSALHERNRTGKGRVVRTSLLASIVGVHAFQGTR
                     YTVAGEVGAAGGNHHPSICPYGLFTCADGMIQLACGSEGLWVKLAEGFGLDPFAPGFA
                     TNRERVGNRELVIETLNAAFADLPHATLMERLAELGIPAGEVRTLDRVYDWDQTRSQG
                     LVIDVEHPVLGQIEIPGPPIRFDDNTFAGGRSEHLHPPRLGEHNERVRAWLDEKDAQ"
BASE COUNT         2486 a         6407 c         6709 g         2731 t
ORIGIN      
        1 ggcctgaagg gcgtcgacgc gtcgctgcgt gaggcagcgg ccgtcgacgg ggccggcgag
       61 gtcaagacgt tcttccacgt catccttccg gtgatgcggc cgatcaacct gatcgtcctg
      121 gtcatcgtcg tcatcgagtc gctgcgagcg ttcgacctcg tctggatcat caacaagggc
      181 cgcaacgggc tcgagctcat cggggcactg gtggcccaga acgtcgtcgg ggaagcgacc
      241 cgcttcggct tcggctccgc gctcgcggtc atcatgatgc tcatctccac ggtgttcatc
      301 gtcatctacc tgcgcatcgt cttccgggag gagcgccgat gagcgccacg accacgtcac
      361 cctcgaccac gggcgcggcc aagccgtccg tccacacggg cgccggcggg gtgatgaaca
      421 agcgccgcgg gaccatcgcg acggtcgtca tgctcttcct cgcgctgctg tggctgttcc
      481 cgctgctgtg ggccctctac aactccttcc gcagctacag ctacacgcag acgaacggct
      541 acgtctcctt cggcggctgg acgttggaca actaccagaa cgcctggagc cgcggcaact
      601 tcgggctgca cctcaagaac tcgctgctca tcaccatccc ggcggtggca ctgacgctgt
      661 tcctgtcctc gatggtcgcc ttcgtcctgg cgcggttctc gtaccgcttc aacctcaccc
      721 tgctcggggt cttcctcgcc gccaacctgc tgccacccca ggccctgctc atccccgtgt
      781 tcaagatgtt ccgcgagatc ccgctgccga ccttcatgag cgagagcggc accatgctca
      841 acagcttctg ggcgctgatc ctggtcaaca ccgccttcca gatggggttc tgcaccttcg
      901 tgctgagcaa ctacatgaag acgttgccct acgagatcta cgagtcggcc cagctcgacg
      961 gcgccagcgt gtggcgccag tactggcagc tgacgatgcc cctggtgcgc ccggccctgg
     1021 cggcgttggc gaccttgcag gtgacgtgga tctacaacga gttcttctgg gccacggtcc
     1081 tcatctcccg cggcgacaag ctgcccgtca ccagtgccct gaacaaccta cgagggcagt
     1141 tcttcgccga caccaacctc gtcgcggccg ggtcgatcat cgtcgccctg cccgtgctca
     1201 tcgtcttctt cgtgctgcag aagcagttcg tggccggcct caccctcggc tcgaccaagg
     1261 gatgagcaca ccgctggtca tcgttcgcgg tgctggttcc gccctcgtcc tcacgcagga
     1321 cgagggcggt gccctgccgc acgtcgtgca ctggggaaca gacctcccgg atgccgtgct
     1381 cggcgacctc gcgcacctca ccggcgcgcc cgtcccgcac aaccagcccg atgccccgtg
     1441 gcacgtctcc gtcctgccca cgtcgggcca gggctggctc ggcacgccgg cgctggaggt
     1501 ccaccgtgcc ggcggacgga ccgctcctcg gtgggtggcc accctcgagg gcggcgacca
     1561 cgacgcgacg gtgcgtgcga cggcatccga catcggtctc gacgtggtcg tgcgctaccg
     1621 cctcgacgag cacggggtgc tcagcgtcga gaccgagctg accaacaccg ccgccgaggg
     1681 tgagcccgtc gacgtggggg cgctgcgcgc cgtgctgccc ctgccgaccc gcgccgacga
     1741 ggtgctcgac ctcacgggcc gctggtgccg cgagcgcgcg ccgcagcggc gcccgctcac
     1801 ggacggcacg tgggtgcgca ccggtcgtcg ggggcgcacc ggccacgacg cgaccctcct
     1861 catggccgct ggtaccaccg gtttcggctt ccgctccgga gaggtgtggg ccacccacgt
     1921 cgcgtggagc ggcaaccacg aacacctcgt cgagcgcctg cccgagggtg ccggcgccca
     1981 cgccgccgtg ctcggtggtg gtgaggcgct gacccccggt gaggtcaggc tcgcgccggg
     2041 ggagacctac cgggcaccga gcgtcgtgtt cgtccactcc accgaggggc tcgacggcat
     2101 cacccgcgcc ctcgtgcgcc accagcgcgc ccagccgggg taccgcacca ccgcgcggcc
     2161 gctcgtgctc aacacgtggg aggccgtcta cttccagacc gacctcgacc acttgcgccg
     2221 cctcgccgac acggctgcgg cgatcggcgt cgagcgcttc gtgctcgacg acggctggtt
     2281 cggatcgcga cgcgacgacc accgcggcct cggggactgg gtggtgtcgc acgaggtctg
     2341 gcccgacggg ctcagcccgc tcgtcgacca cgtcaagggt ctggggatgg agttcgggct
     2401 ctggttcgag cccgagatgg tcaacctcga ctccgacctc gtgcgcgcca acccgcactg
     2461 ggtgctcggc ccggtggcgg gcgcgccgct gccctcgcgc caccagcacg tgctcgacct
     2521 ggcgaacccc gacgccttcg cgcacgtcct ggaggcgatg agctcgctcg tggcggagta
     2581 cggcatcgac ttcatcaagt gggaccacaa ccgcgacctg cacgaggtgg tgcgcaccga
     2641 cggctccggc acggatcgac ccgccgtgca cgcccagacg agcgcgacct accggctcgt
     2701 cgacgagctc aggcggcggc atccgggtct ggagatcgag tcgtgctcca gcggtggggc
     2761 gcgtgtcgac ctcggcgtgc tcgagcgcac cgaccgggtg tggacctcgg actgcaacga
     2821 cgccctcgaa cgggtggcga tccagcgctg gaccgggctg ctcgtgccgc ccgagcggat
     2881 gggcacgcac atcggcccgc cgacggcgca caccacccac cgccacctcg acctgtcgct
     2941 gcgcatgctc gtcgccctcg gcgggcacgc gggactggag tgggacatca cgacgtgcag
     3001 cgccgacgaa ctcgacgcgc tgcgggcgtg gtcggccctc taccgcgagc tgcgaccgct
     3061 gctgcacggt ggggatgccg ttcgctcgga ccacccggac ccggccctcg tcgtcggcgg
     3121 ggtcgtggct cctgaccggt cggccgcggt gttcagcgtc gtccaggcgg tgtccggtcg
     3181 cggggtcgca cccggggtcg tgcccttgcc ggggttggat gccgggcgtc gctaccgggt
     3241 gcgggtgcgg tcggaagccg ggttgccggc cgtgatccag gtgcatccac ccgcgtggtg
     3301 ggatgccgcg ctgggcgacg ggatcgtcgt cagcggggcg gcactcggcg aggtcgggtt
     3361 ggccctgccc gtgctggcac cggcccaggg attcctcctc cacctggagg gcgtcgacgc
     3421 ctgagggccc ggctggtgac agactcggtc ggtggccatc cctccttcgc acctgtcgtt
     3481 cgccgctctg gagttcatga cggagcggca cctcgcgacg ttgaccaccc tgcgggctga
     3541 cggatcaccg cacgtcgtgc cggtggggtt cacctgggat gacgacctgc tcgtcgcgcg
     3601 cgtcatcgcg agtcggagca gcgccaaggt cgtccaggcc cgcgccggcc gccgggcggc
     3661 gctgtcgcag gtgcaggccc gtcgttggat caccctcgaa gggtcgacga gggtgctgga
     3721 ggacccggca tccgtgcgcg atgccgagca gcggtacgcg cagcgctacc gcgtgccgcg
     3781 cgagaacccc gagcgagtcg tcatcgtgct cgacgtcgac cgggtgctcg gtggggactg
     3841 gccgcacccg ctgccaccct cgccgtacgc gacctgagcc gggcagcgtc gaccagctct
     3901 ccccggaccg cgccgggaaa gattctttgg cacggtgcat ccggggtcgc ccccttcacg
     3961 gaatgactgt cagcgggtgc agagaggcac cccaccaact gaagggacgc atcatgaagc
     4021 gctttctcgt cgcaggggca gttgccgctt tcgccatggt cggcttctcc agcaccgccc
     4081 aggccgcacc ggcacaggcc gcgtgcttcg gccaggtcca caagctcgtg aacagtggtg
     4141 gcctccccgg tatcgacaac gtcgggcagc tcgtcggcgc cctcggtggc ggccagaaca
     4201 agaacgccac cgccaagacc ttctgctgac cgcccgccag acccggcgct gagcgctcgg
     4261 gcccacacgc cagaggcggc tcggaccacg tggtccgagc cgcctcttcg cgtgctgttg
     4321 tgccctggtg ccttacggct gcgggacgat gaactcgtcg agcaggggca gggtgccctc
     4381 gcgcaggtcg gcggtcttct ccggcttgat ctgacccggg gcgaagctgg ccatcgggcc
     4441 ctcgatcggc aggttcaacc gtgaggtggc gaggttgacg ctgtacgtct gcgccgaggt
     4501 gtcgatggcg gtgacgcccg agcggttgcc gctgatcacg aggccgagct ggtggccggc
     4561 ggggacgacg acgtcgttgg cctggagctc gacggtgacg ttcttcgtgc cctcaccgtc
     4621 gaggcggatc cagccgcggg cgaggacctg cagctcggtg ggcccgaccc ggcgggtcac
     4681 gtcgcggtag caggcgtcgt cgacggcggt cgaggcgccc cagcagctct cggtcgtgag
     4741 ggtcagggcg ccgtcgttgg tcgtgagcac acggtccatc gcgccgtagt cgacgagctg
     4801 caccgagagc tgcccgaccg ggcggccctg ggtgacctcg aggtcgacgg tgggggtgcc
     4861 cgagatccgg accgggagcc cttgctgggc cagacgtccg actggaccca gcggtcgggg
     4921 gcgacctcga cgtcgacggc aggctcgttc aggatgccgt tgtccacctg catcagctcg
     4981 tggtcgaacc agcggtgcag cgtgtcgacc caccggtcac ggtcggagtc gaacgggtcg
     5041 acgtgcccga gccgggtcag ccacatcttg cggtcgacgc cggcctcgcc gaggtcctgc
     5101 caccacgtcg acgcgttgcg catgcggacg ttgttgtcct gcagcccgtg catgatgaac
     5161 acgctggcct tgaccttgga gacgtcgtag aacgagccct cgcggtagtc ccgctcggcc
     5221 cagaagtcgg tgtacgcacc ggtctcgtcg ccgtcgttgg cgttcatgaa ggcgttggtc
     5281 gccacgcagt tcgtggggac cgtgcggcgc tgggcgatcg tcgtggacag gctgctcggg
     5341 tagttgttcg acttcacggc gttctggtag cggttgtagt cgtaccagga gctgatcgcg
     5401 ctgatcggga cgatggtctc gaggccctcg accccggtgg ccgcgacgcc gttggcgagg
     5461 gtgccgtcgt aggacttgcc gatcatgccg gtgcgtccgt tgctccacgt ggcctcgacg
     5521 acctcgccgg cggcgttgcg ggctaccgcg ttgccgttga gccactcgat gacgaccttg
     5581 atcgactcga tgtccgagcg tccgccctgg tcggcgcagc cggtcgagcg gccggtgccg
     5641 gccatgtccg gggcgacgaa cgcgtagccg cgtggcacga agtagttgtc gtagaacagt
     5701 ggcatcttcg ccggcttgcc gtcggcgtcg taggacttga actcgccgtc gttgccgcgc
     5761 ccgaggctga ggtagtaggg gctggcgtcc atgacgaccg ggatccgtgc ggtgccgtcg
     5821 agctcgcgcg ggcggatgat gtcggtggcg atgcgctcct gcgcgccgtc accgtcgagg
     5881 tccggggcag tgacccagac cgtctcgcgg atcgcatccg cgtactcgaa gaccggggcg
     5941 gtgacgccgt cgacgatcgt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 ngtgggcctt gacggtgctc gtggtgatga agagctcgct cgcgatctcg tcgttggtcc
     6121 ggccccgggc gacggtgacg agcacctcct cctccctcgc ggtgagcggc tccaggggct
     6181 gtcgggccgc ttccgctcgc gaggtggcga aggtggcgag cagccgcacg gtgacactcg
     6241 gtgcgatgag cgcatcaccc tcggcggccg cgtggatcgc ccgggtcagc aggtcggggc
     6301 cggcgtcctt gagcaggaag ccgcgggcgc cggccttcag ggcgccgtgg acgtactcgt
     6361 cgaggtcgaa ggtggtgatg acgatgatgg gcagcgggtc ctcgacgtcg gggccggcga
     6421 gctggcgggt ggcctcgatg ccgtccatca ccggcatccg gacgtcgaac agcccgacgt
     6481 cgggccgtaa ctgtcgtgcc agtcggacgg cttcgcgacc gtcggcggcc gcaccgacca
     6541 cgtcgatgtc gggctgggcg tcgagcagca tcgtcagccc gctccgcacg atgggctggt
     6601 cgtcggcgac gaggaccctg atgctcatcg ggccaccccc gcccggggga ggttcgcctc
     6661 gacgcgccaa ccgcggtcgg cggtgggacc ggcggcgaag gtgccgccga gcagggtcac
     6721 ccgctcgcgc atgccgacca ggccgtagcc gtcgggggag cggtggggcg agccggtgga
     6781 gccgtcgtcg tcgacggtca gccgcacgtg gtcggtctcg ccggtgacct gaacggtgac
     6841 gcgggtggcg tggcgggcgt gacgtctgga gttggtgacg gactcttggg ccagtcggta
     6901 gacggctgcc gcgacgctcg ggctgaggtc cgtgagctca ccggtgaggg tcacctcgac
     6961 acggggctgc gcggccccgg tggtggcgag ctgctcgatg tcggccatcc ccggctgggg
     7021 agtcaacgag ctgtgccgcg cgtcgcgcag gacgccgacg atggcgcgca gttcggtgag
     7081 ggcccgggtg gcggcctcct cgatcgtggc caaggcgtcg acggcacgtt cggggtgggt
     7141 cgcggcgacg gcccgacccg cctgcgcctg gatcgcgata cccgacacgt ggtgggcgac
     7201 gacgtcgtgc agctctcggg cgagctgctc gcgttcgcgg gccttggcct cctggatgtc
     7261 gcggatgcgg atccgggcgc ggtagcggac ggcggcgccg agagctgccg cgaagaagaa
     7321 gaacgcgtag ccggtgatcg tatcggtcag ggtggtcacg tcggcgacga aggtgatggc
     7381 cagccacgtg aggatcacga gcagcccgcg aacggcatcc cgacccgacc cccaccggaa
     7441 caacgcatag gtgaggacca acgtggccga cacgctcgag gtcagggcgc cctgagtgcc
     7501 cgtggccatc cggaagaggt cgacaacgat cagcgtgccg aaggccacga ggactgcggg
     7561 gagcgggtgg gagcgccgcc aggggagggg ggcgaccgcc gcgagcgtgg cgatgaggag
     7621 cacgggtgca ggcgccatgc cgtcacgaag gaccgcctcg ttgacagccc acccgacgaa
     7681 ggcggcgacg agggcccagt cggctgcact ggtcgccgga gggttcgtgg gtcggggttc
     7741 gacccacagt gagcgcagtg cgtcagtggc catccggcca gcgtaggcag cgggcgtcgc
     7801 ccgcgtatcc gccgaaaggc caaccgggtc ttgggccaac ggacagggca cgtcgggtct
     7861 gcgggctgat gtgggcgcgc acggcatccg tgagggttgt gaaggtcacc aaccccacga
     7921 cccgacgaca ccggaggccg ccatgagcac ttctcgaccc gacaccgcgc tggacgacgt
     7981 acgcctgccc ctgaaggcca ggctcgccgc agcctggacg agcttcatgt tcctctacgt
     8041 ctacgtcgac cacctctcgc tgtacaagcc cggagtcctc gacgagctgc tggccgggcg
     8101 ggtgcacgag ttcgacaccg ggccgacgtt catcggggca gggctcacgc tcgtggccgt
     8161 gccgagcctg atgatcgtgc tctcgatgac cctgccggcc cgggtgaacc gggtggtcaa
     8221 cctcgtcgtc gcggcgctgt acctgccggt caccgtgtac aacgtcgcgg gcgaaccgat
     8281 gtcgtacgcc tacttctacg ggctgtccat cgggctcgag gtgctgctgc tggcgtacat
     8341 cgcgtggtcg gcctgggcct ggccgcgcgt tgcggccccg gccgccgggg ccgccggggc
     8401 gggcctgcca accggcaaga gtgccaactc cccggttggc tgagcgtgag gcggggcggg
     8461 cgcgacggcc tttgccggtc ggtgagcccg cccgccccgg ccgtcatgac gggcggcgcg
     8521 aactcgcccc gtatcattgg gggtgacggc ggggatgccg ccgggtcagg aggccacgat
     8581 gacgacgttc gctggtgatg gcggcctgtt cgtgagtgat ctgctggtga tcgggctcct
     8641 ggtgctgctg gctgtcgtcc tggggttcgg cctgaccgtg acgttcatcg tgtggcggct
     8701 ggttcggggt gtgcgccgct gggtggcccg gcgacgggga gtggctgccg cgcaaccgcg
     8761 gatggtgcgc aagcccgggc ccgcgcgtga cgtcgccaag ctgcgtcgag aactgagcga
     8821 cgaggtgtcg gcggccgagc tgatgctgtc tcgcgccaag aacggcaggg tgttcgtcgc
     8881 cgacgcgcag accctgctcg acgacctcaa ggtcggcgcc gcatcgctcg atgccaacat
     8941 ggccagcgtc cagaactact cggatgccgc tcagcagcgc gcggccctgg aggcgctgcg
     9001 gccccaggtc gcgaagctca tcgagaccag ctacctggcg cggcagacct tgctgcagac
     9061 cgagatcgcc gaccgcgaac gacacctcac cgacctcagc gaccacgtgg agcggcaggc
     9121 cgaggccttg aagatctacc gcgacgagtc gcacagcctc aacctcgacg acctgcgcga
     9181 gcacccggcc cgctaggcgg gcacgagggg tgccaatcgc gcggtgggtc gcgcccttgg
     9241 catccctact ggagatgtcg cgcacgggct ggggtgccaa tcgcgcggtg ggccgcgccc
     9301 ttggcacccc ctcgactcgc cttggcacgg cccgtgccgc cgcgtcggta gactggcccc
     9361 agacgactgg cgagggtggg gcaccaccgg gaagtcacac gtcgggacgc atcgcccgcc
     9421 tgggtgcccc gcagcgtgac cgacccgacc ccgcaaggag cccaccatgg ctgtcgaagt
     9481 gtccaccccc ggccacacgc aggacgtccg gccggtgcgc cgcgccctga tctcggtgtt
     9541 cgacaaggca gggctggacg acctcgtccg cggcctccac gaggccgggg tcgccctggt
     9601 ctccaccggt gggtcggccg ccctcatcga gtcgctcggc ctgcccgtca ccaaggtcga
     9661 ggagctcacc ggtttccccg agtgcctcga gggccgggtc aagacgctgc acccgctcgt
     9721 gcacgccggc atcctcgccg acacccgcaa gcccgaccac gtcgcccagc tcgccgagct
     9781 cggtgtcgag gccttcgacc tcgtcgtcgt caacctctac ccgttcgccg acacggtggc
     9841 gtcgggggcc gcggctgacc agatcgtcga gaacatcgac atcggcggcc cctcgatggt
     9901 gcgcgccgcg gccaagaacc accccagcgt cgccgtggtc accgacccgt cgacctacgg
     9961 tgacgtgctg gatgccgtgc gcggcggcgg gttcacgttc gcccagcgtc agcgcctcgc
    10021 ggcccaggcg ttcgtgcaca ccgcgaccta cgacgtggca gtcgcgtcgt ggatgggcaa
    10081 tgcctacacc gacacctcgg agggaaccgg cttcccggcc tgggccggcg cgacgtggga
    10141 caaggcggcg gtgctgcgct acggcgagaa cccgcaccag cgggcggccc tctacaccaa
    10201 cggtttccag ccccagcccg gcctcgcgca ggcgacccag ctgcacggca aggagatgtc
    10261 gtacaacaac tacatcgacg ccgacgccgc ggtccgagcc gcctacgacc atggcgacca
    10321 gcccacggtg gccgtgatca agcacgccaa cccctgcggc atcgctgtcg gcaccgacat
    10381 cgccgatgcc caccgcaagg cccacgagtg tgacccggtc tcggcgttcg gcggcatcat
    10441 cgccaccaac cgccccgtga ccgtcgagat ggcccgcacc gtcgccgaca tcttcaccga
    10501 ggtcgtcgtc gcccccgggt tcgacgacga cgccctcgag gtgctcacgg cgaagaagaa
    10561 cgtgcgcctc ctgcggtgcg cggcccccgg ccgcggtggc gtcgagacgc gccccatcag
    10621 cggtgggctg ctcatgcagc agcgcgacgg cgtcgacgcc gtggtccgcg gcgacgacga
    10681 cacggtcagc ggcggcgacg acgtcgtgaa ctggcgcctg gtctccggcg atccggccga
    10741 cgaggcgacc ctggcagacc tccagttcgc ctggcgggcg gtgcgcgcgg tgaagtccaa
    10801 cgccatcctg ctcgcccgcg gcggtggctc ggtcggcatc ggcatgggcc aggtcaaccg
    10861 ggtcgactcc tgccacctcg ccgtctcccg cgccggtgag gaccgcgcgc gaggtgccgt
    10921 cgcggcatcc gacgcgttct tcccgttcgc cgacgggctc caggtgctgc tcgacgccgg
    10981 cgtgcgcgcc gtcgtcgccc ccggtgggtc gatgcgcgac gcagaggtga tcgaggctgc
    11041 tcaggccgcc ggcgtgacca tgtacttcac cgggacgcgg cacttcgccc actgaggcac
    11101 tgggcgtccc tgcggcgacc tatcggtcga tcggggggac gggcgcggcg catcggggtc
    11161 agcgggtggc ggcgtgccgg ggggtggtgt ccggcacgcg ccgcgggcgc agccggtgca
    11221 cgagccactc gagcggaccg gtgccccacc agcggtccag ggcccagcag gcaccgacca
    11281 cgacgacgag gctcgagccg aggatccacc acgcgtcgtc cggggcgccg tcacgcagga
    11341 ccgacagcag cgcgagccag aggatctgca cggtgtaggc cgtcagggcc agccgtccag
    11401 tcgccagcac cggctcgagc acccggcgca gcgagaaccg ttccgcaagg gcgagcatga
    11461 tgaacgacgc caccacgctg cccgtcgcca ggagggtggc cccgatgacc tcggcggtgg
    11521 tgccgctgta cgcggccgag ccggatgccg tccccgcgcc gagacggtag gccgccaggc
    11581 tcgttgcgaa cagcgccccc gccacgaccc aggcctgcgg caggtgcgtc acctggtgca
    11641 ggaccgccgc cagggccagc cctccgagtg ccatgggcag cagggtggcc agccggtagg
    11701 cgtcacccgt ggccacccac cccatgaggt cgtccagcca ccccggccac gtcgcgatgt
    11761 tctcgttcgc ggcgagtcgg gcccggtcga cgaccaccgg tgccacgacg gcgaggccaa
    11821 ccgcgagacc cacggtgagc accggcatcc ggcgcagcag cagggccagc ggtgccagca
    11881 cgatgacgag cacgccgagg tggggcaaca cgatgatgac ctggtcgtgc agccgctgca
    11941 gcaggatgcc gagcaggatg aggatgaggc cgcgctggag gttgtcgccg aggaaccggt
    12001 cggggtgggg ggagcggtcg cgcaggtgga tgcccatcga cgtgccgatg atgacggcga
    12061 agagcggcgc cgtgaggtac tccgtgatgg tggcagggcc gccgaccggg ctgaggtgcg
    12121 cgacgagcat cgacacgacg gccaggccgc gagcggcatc cagcccgtcg aggcgggtgc
    12181 ggcgcccgtg gtggccggcc gccgggatgg tcacggagac gcgcatgggc gaagtctgcc
    12241 ggtcgagagg aggcaacacg ccgtctgcac catgatgcag acggcgtgtt gcctcctctc
    12301 gacgaggtgg gtcaggtcgt cggggggcgg taccccgtcg gctcacccga gcccaggtcg
    12361 acgatcgccg cgaccgggcc gccaccctca gggccctggt gcgcggccga caccgacacg
    12421 aacaccgccg ggtcacccgt caccgaggcg gtgacgccac cgacgcacga cttgatctgg
    12481 cggtgccagt gcacgtcgga gtcgtcgagc atcgcgttgc ggcgaccggc gacctggccg
    12541 tcgaggctgg cctcgcactt gaggaagacg ttgaccaggc gcccctcgag gtcgctgcgg
    12601 tgcgggcgct cggggaggtc gaggccggca tccttgatcg ccgcccagat gccgtcctgg
    12661 tcgagggcgt cgttcatcac cgagtggccg atgcggtagc gaccgccgac accgcgcgcg
    12721 ttgccgacga cgacgacctg cgcctggtcg agctcgacgc ccgaggagca cgaggcgacc
    12781 gaggagaaca gctcgcggtt gtgcatgacg tcggcatcgg tcggcatctc gatctcgccg
    12841 agggcgacgg cgatgccgag cgcggtgcag ccgttggaca ggtccatcga ctcgtgggtg
    12901 tgctcggtcc agacctgctt gccgcgggcc ttcgcgtcac ggatggtgtg gatggtcagc
    12961 agcggggtct tggtctgcac gtagtgcacg tcggccgggt cggtgatgcc ggcgcgctcc
    13021 atggcgacct tcacggcatc cgcgaccttg gagaccatcg aggtgtaccc gatctcctcg
    13081 ggcagcaggt gctcggacat cgcgaacccg acggtcaggc gcggctcgtc ggtctgctcg
    13141 gccttgtcgg cgggcacggt cgcgaagatg gtcgcgtggg ggctgatcac gccgtcggtg
    13201 ccgcccgacc acacgatcgg gatctccttg acggcggccg ggtcggcacc cttctcgacg
    13261 aggatctcgc ggaaggcgcg gtcggcgatg atgcgggtgt agtcgttgac cccgccgttg
    13321 ccctcggtct tgccgatgac cgcgatgacg cggctggcct cgaacacgcc ctcgtcgatg
    13381 agcttgccca gctcggaggc gtcggacacg gtgtgcagcg ggaccttgcg gacctcgatg
    13441 gcgtcaggca cggtgcgggt tcctttcgtg gatgccgggg ggcggctcgg tgggtctggt
    13501 gcggttgggg gtcagtcggg gtcgacgacg gtgccgaagc tgccctcgac ggcgtcggtg
    13561 atgtgggtga gggacgtgat gacgctgcgc ggcccgccgc cggcgacgaa gcggatggcg
    13621 gcgtccacct tggggcccat cgaccccgac gcgaactggc cgtcggcggc gtgggcccga
    13681 agctgcgaga ccgtcacgcg gccgaggtcg cgctgggtgg gcgtgcccca gtcgacggcg
    13741 gcgtggtcga cgtcggtggc gatgacgagg acgtcggcgt cgatggtgcg ggcgagcagg
    13801 gcagcggtga ggtccttgtc gatgacggcc tcgacgccgc gcagccgacc ggcggggtcg
    13861 cgcacgacgg ggatgccgcc gccgccagcc gcgacgacga cgtggccggc ctcgagcagg
    13921 gtgagcaggg ccgggacgtc gagcacctcg aggggttcgg gggaggcgac gacgcggcgc
    13981 cagcctttct cgccgcggtc ctgccagacc tggccgtggt cgatcatcgg ctgggcctgc
    14041 gcgtggctga ggtagcgacc gatgggcttc gtggggtggg tgaacccggg gtcgtcacgg
    14101 tcgacgaggg tgcgggtgac gatggctgcg accgggcggc gagtgccagt ggcggccagg
    14161 gcgtcgtcga gggcgtcgag gatcgtgaag ccgagggtgc cctgggtctg ggcgccgcac
    14221 cagtcgagcg gcaccggcgg gacgacgtgc gcggcgagct cgttcttgac cagcaggttg
    14281 cccacctggg ggccgttgcc gtgggtgatg acgagctcgt gaccggcggc gacgagacgg
    14341 gctgccgcgg tcatcgcgac ctccaccgca gcgatctggg ctgccggggt cgcgctgccg
    14401 tcgggcgcgg tcatggcgtt gccgcccagc gcgagaacta cccgcatggc acgccctcct
    14461 tggctggctc tgctcgtggt tccggggggt cccggggtct cgacactaga agccgccgca
    14521 gaggcaggcg ttgtcaggac ctgcccaatg gggctccatc ggttggcaag ttgttgcgcc
    14581 ccctctcggc gatgagtgcg gaagtggctg ccccggcaac cacttccgca ctcacggcga
    14641 tggatgaggg atgctgtgcg gcatgactgc tcgccgtgct gctgaccgca cctggctcgt
    14701 cgcgctcgcg gcctcgctct ggggtctgtc ggccctgtgg cggggcccgc tcgccaagga
    14761 gtacccggcc ctggccatcg tcttctggga gcacctcgtc ctcgtcgtgc tcgtcagccc
    14821 gtggatcgtg ccggccgtgc gccgggtcct cgccgccagc acgcgcacca aggtgagcgt
    14881 gctcgtcatc ggcgcgggct cctcggccct cgccacgacg ttgttcaccg cggcgttccg
    14941 gctcggcgac cccatcacgc cgcaggtgct gcagaagctc cagccgctca tcgccatcgc
    15001 cctcgcagcg ctggtgctgg gggagcggct gcgccgcagc tacgccgtct tcgccctgcc
    15061 ggccatcctc ggtgcgtggc tgctggcctt cgccgacccg atgggcgtca gcgtgtccag
    15121 tgcccaggct gccgccctgg ccgtcggcgc cgccgccctc tgggccgccg gcaccgtgct
    15181 cggccgggcc gccagtgccg agctgcgctt ctccgacctc accgcgctgc gcttcaccgt
    15241 cggcctggtg accctgctcg ccatcagcgg tgtcacgtcg accccgctcg ccatcggggt
    15301 cgacgcggcg ccgcggatcc tcctgctcgc cctgttcccc ggtctgctgg cgctggttct
    15361 ctactaccgc gcgctcggac acacccccgc ctcgcgggcg accctggccg aactcgcctt
    15421 cccgctcact gcggccgtcg tcggcatcgt ggccttcgac gcgagcctca ccgggtcgca
    15481 gtgggtcggc ttcgcgctcg tcctggcctc cgtcgtcggc ctcgcactgc acgagcagcg
    15541 cagcagccgc ccgtccgtcg cggtgcccga cgacgcccgg gaggcgctca ccgccgcccg
    15601 ctgaccggca tccggatgcg cggctacagg gcgcgccagc gggcgtgccg ggcctggagc
    15661 cgggtctccg gtgggagggc gacgaggtcg ccgagctcgt gccggatcgc ctgcgccatc
    15721 cggcggcagt aggcctcggg ctcctgggcg gcgtccgggt gctcggcgac gatgcggtcg
    15781 acgatgccgg cctcgagcag gtcgagtgag cgcacccgtt gctgcgtcgc gagctccggg
    15841 gcgcgatcgg gcgtgcggtg caggatcgcc gaggcgccct cgggtggcag cggcgagagc
    15901 cacgagtgct gggcgcacac gacgcggtcg gcgggcatga gcgccagcgc cccaccgccg
    15961 gtgccctgcc cgagcagcac gcacaccgtc ggcaccggga gcacgacgag ctcggccagg
    16021 cagcgggcga tctcaccggc catgccaccc tcctcggcct ccttcgacag cgccgcaccg
    16081 gcggtgtcga tgacgctgac gagcggcagc ccgagctcgg tcgcgagctt catgccgcgc
    16141 cgggccaccc gcagacccga ggggccgagc ggcgcctccg cccgctcggc gcggcggtcc
    16201 tgcccgagga cgacacacgg catccccccg aagcgcgcca gcgcgaggaa gaggctgcgg
    16261 tcccactcac cggcgcccgt gccgaacagc ggcagcacgt cggtcgcgcc gagcttgagc
    16321 agggcgcgca cccccgggcg ctcggggtca cgcgagcggg tgaccgactc ccacgcgggc
    16381 aggtcgggca gcggctcgcg cggcaccgcg ggcacgggct cggtgtcgtg cggtgcgagc
    16441 acgaggtcga ggaaccgacc cgcgatcgga gggagcgcct cgacgggcag cacggcgtcg
    16501 agcagcccgt gctcgtacag gttctcggcg gtctgcacgt cggccgggaa cggctcaccg
    16561 tagagggcct cgtagacccg ggcgccgagg aagccgatcg tcgcgcccgg ctcggcgacc
    16621 gtgacgtggc cgagtgaccc ccacgaggcg agcaccccgc cggtcgtggg gtggcgcagg
    16681 taggtgatgt acggcagctg cgccgccttg aacgcggcca ccgcgcccga gatcttcacc
    16741 atcgtcacga acgccggggt gccctcctgc atgcgtgtcc cgccggatgc cggggcggcg
    16801 aacagcggga cccgctcgcg ggtggcccgc tcgaacgcga gcgtcacccg ctcggccgcc
    16861 gcgcgcccga tcgagccggc gaggaaggtg aactcaccga cgatcacggc gacccggtgc
    16921 ccgtggatgc gcccttcacc ggtgatgatc gactcgtccg tgccggcccg ctcagcggct
    16981 gctgcgagct cggcggcgta ggtgcttccg ggtggggcaa cctcgagcgg ggcctggtcc
    17041 cacgagacga acgagccctc gtcgagcacc tcggccagca gctcgtgcgc gccggggcgg
    17101 cgcgggcgct cgctcactgc gcgtccttct cgtccaacca ggcgcgcacg cgctcgttgt
    17161 gctcaccgag ccgtggcggg tgcaggtgtt cgctgcgccc gcccgcgaag gtgttgtcgt
    17221 cgaaccggat cggcggcccg gggatctcga tctgcccgag caccgggtgc tcgacgtcga
    17281 tgacgaggcc ttggctgcgg gtctggtccc agtcgtagac gcggtcgagg gtccgcacct
    17341 cgcccgcggg gatgccgagc tcggccaggc gctccatgag ggtcgcgtgg ggaaggtcgg
    17401 cgaacgccgc gttgagggtc tcgatgacga gctcgcggtt gccgacgcgc tcgcggttgg
    17461 tggcgaagcc gggggcgaac gggtcgaggc cgaaaccctc ggcgagcttg acccacaggc
    17521 cctcgctgcc gcaggcgagc tggatcatgc cgtcggcgca ggtgaacagg ccgtacgggc
    17581 agatcgacgg gtggtggttt cccccggccg ccccgacctc accggccacc gtgtagcggg
    17641 tgccctggaa ggcgtgcacc ccgacgatcg aggcgagcag cgaggtgcgc acgaccctgc
    17701 ccttgccggt gcggttgcgc tcgtgcaggg ccgagaccac cccgtaggcg ccgtacatgc
    17761 cggcgagcag gtcgccgatc ggcaccccga cgcgctgcgg gtgctcgggg tcggcgcccg
    17821 tgagggacat cagcccgccc tcgccctgcg cgatctggtc gtagccggcg cggccaccct
    17881 gcgggccgtc gtgcccgaaa ccgctgatcg agagcagcac aaggccgggg ttgagctcgt
    17941 ggaggcgctc gacgctgaag ccgagccggt cgagcacccc gggacggaag ttctcgagca
    18001 gcacgtcggc gtgggtcacc aggcgggcga gcgtctcgcg gccgtcgtcg gtcttcaggt
    18061 cgagggtcac cgactccttg ttgcggttgc acgacaggaa gtacgtcgac accggctcgt
    18121 cgtcgggccc gacgaagggt ggcccccagc cgcgggactc gtccccgcca ccggggttct
    18181 cgaccttgat gacccgagcc cccatgtcgc cgagcatcat cgcgcagtgt ggcccggcca
    18241 gtgctcgggt gaggtcgatg acgacgacgt cgtcgagggg tccggccacg gtggctcctg
    18301 gcgtcgctgg aaggagaggg ggatgccgtc aacctagcca cgcggcatcc ggcgtcgttc
    18361 ggcagggcgg gccgtcattg gccgtgaatg gctgtcaggt tgtgccagcg ggatcgtccg
    18421 tgacgtggcg ggt
//
LOCUS       DAOSOC010000031        17760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3212_length_17760_cov_10.464050, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000031 DAOSOC010000000
VERSION     DAOSOC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 17760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17760
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3212_length_17760_cov_10.464050"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..469
                     /gene="rpmH"
                     /locus_tag="PL014_04250"
     CDS             332..469
                     /gene="rpmH"
                     /locus_tag="PL014_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOT56333.1"
                     /translation="MSKRTFQPNNRRRAKTHGFRLRMRTRAGRAILAARRRKGRSELS
                     A"
     gene            478..843
                     /gene="rnpA"
                     /locus_tag="PL014_04255"
     CDS             478..843
                     /gene="rnpA"
                     /locus_tag="PL014_04255"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOT56334.1"
                     /translation="MLPAPNRLRERSDFTSAVRSFGGCRAGSRLLVVHATSTGARAGL
                     PPRVGFVVSKAVGGAVVRNRTKRRLRALVAQRLDGIPTSVDLVVRANPPAGQASSREL
                     QQALDPLLRRVVKELGGVA"
     gene            840..1208
                     /gene="yidD"
                     /locus_tag="PL014_04260"
     CDS             840..1208
                     /gene="yidD"
                     /locus_tag="PL014_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOT56335.1"
                     /translation="MSIGRVLASPLIGLIRLYQNVISPMRPPTCRYYPSCSAYAVTAL
                     QTYGVLRGSAMATRRLLRCHPWSPGGVDHVPLADPVTGRPIPGTTGPRDIGSHSAADL
                     RSSDAEAAANAAPRHPSHPH"
     gene            1268..2341
                     /gene="yidC"
                     /locus_tag="PL014_04265"
     CDS             1268..2341
                     /gene="yidC"
                     /locus_tag="PL014_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOT56336.1"
                     /translation="MGDAFNAILAPIEWLVAWIMYGFHTALTAIGMPSGSGWTWALSI
                     VGLVLVMRAAMIPLFVKQIMASRKMQMIQPELQKIQKKYKGKTDPDSRQAMTQETMAL
                     YKKEGTNPFSSCLPILVQSPFFFGLFRVLNGVDEIAAGTTDKIGPITQPVAAEFEKAS
                     IFGAQLSETFLGSDSLNVKIVTAVLIVLMSATTFLTQRQLMTKNMPASALDNPFAKQQ
                     KLLLYILPLVFAVSGVNFPIGVLIYWFTTNVWSMCQQFYVIRRMPAPGSAAEASYHAR
                     LAKKGKPIPGAAAAASATAAAEAEAIADETAKKSGQRQQPTGKKRKKAKGAGGSAPAG
                     GKPSGGATPKDTPPSDSAKPSTS"
     gene            2369..3049
                     /locus_tag="PL014_04270"
     CDS             2369..3049
                     /locus_tag="PL014_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOT56337.1"
                     /translation="MSENLTQDPARNDAEPEQPTDVESTSEHDESVSDVVEADVQAIT
                     GGDVDADTLDTASDVDVDVTPAASTSRQGQLEREGEVAADFLETLLDICDLDGDLEVD
                     IDGDRAAVSIVDSEDGRVPRRLVGQNGQVLDALQELTRLAVQSATGERSRLMLDVGGH
                     RAERRASLVTLAKEAIEEVRTSGERKPLTPMTAFERKVVHDEVLAAGLVSESEGAEPR
                     RYVVILPA"
     gene            3063..3806
                     /gene="rsmG"
                     /locus_tag="PL014_04275"
     CDS             3063..3806
                     /gene="rsmG"
                     /locus_tag="PL014_04275"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOT56338.1"
                     /translation="MKHDAGDAAPIASAPAAPEGAAGLFGDRMPLAEQFAAILADTGV
                     SHGLIGPREVPRLWERHLLNCAVIADALPREARLIDVGSGAGLPGVVLAIVRPDLEVH
                     LVEPMLRRTQWLEGVVADLGLASVTVHRGRAEEFVGVLAAPYVTARAVARLDKLARWC
                     VPLLEPQGVLVAMKGRSAATELEEDRKGLQRLGLTDAVVTEHVSALLEEPVLTVDLRF
                     APKPARTKAARREGARSSKQARAARKAPS"
     gene            4362..5384
                     /locus_tag="PL014_04280"
     CDS             4362..5384
                     /locus_tag="PL014_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT56339.1"
                     /translation="MGDVSRETSTPARTATAPSGLPREEEVAREQIAAVLPDADAQTP
                     LATAVANDARKRITLSGRTVPRPPRTRILTVANQKGGVGKTTTTVNVAAALSQAGSTV
                     LVIDLDPQGNASTALGIPHHAEVPSVYDVLVDGRPISEVVQECADLPGLVCVPATIDL
                     AGAEIELVSLVARETRLQRAVTTYVEERAAAGDPVDYVLIDCPPSLGLLTVNAFVAAS
                     EVFIPIQCEYYALEGLSQLLKNIELIRNHLNPSLHVSTILLTMYDGRTRLAAQVADEV
                     REHFPQQTLRAVVPRSVRISEAPSHGQTVMTYDPNSSGALSYLEAAIELAERAAATPN
                     TTEESA"
     gene            5381..6496
                     /locus_tag="PL014_04285"
     CDS             5381..6496
                     /locus_tag="PL014_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOT56340.1"
                     /translation="MSEKRRALGRGLGALIPSGPATDRPIDVFFRDQDPVTPPSTVPV
                     DDSGQSASESGPHSGDLSGANGAPSTDSDEPTVEAPPSDDGAAATDTGLRPVPGAEFA
                     EIPLDHIRPNPRQPRTVFDEDELAELVHSIREIGVLQPVVVRRIPTEAQDGEARFELI
                     MGERRWRASREAGKDVVPAIIKVTEEDDLLRDALLENLHRSQLNALEEAAAYQQLLDD
                     FGCTQEELARRIGRSRPQISNTLRLLKLPPLVQRRVAAGVLSAGHARALLGMSDSAAM
                     ERMAQRIVAEGFSVRTVEELVTLGDDDTAPVARRAPRAGARRPQLDDLAADLSDRLET
                     RVRIALGQRKGRLTVEFASVDDLNRILDAMHLRDPRD"
     gene            complement(6582..6905)
                     /gene="trxA"
                     /locus_tag="PL014_04290"
     CDS             complement(6582..6905)
                     /gene="trxA"
                     /locus_tag="PL014_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT56341.1"
                     /translation="MGAVSATSDATFEADVINQDRPVLVDFWAPWCGPCRAVAPILDE
                     IARDHDEVSIVKLNTDENPAITGQLGITSIPTMHVYVGGEIVKTIIGAMPKPKLLREL
                     EPFLG"
     gene            complement(7020..7958)
                     /gene="trxB"
                     /locus_tag="PL014_04295"
     CDS             complement(7020..7958)
                     /gene="trxB"
                     /locus_tag="PL014_04295"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOT56342.1"
                     /translation="MCPVTSDVRNVIIIGSGPAGWTAAVYAARANLEPLMFEGAVTAG
                     GALMNTTEVENFPGFRDGIMGPDLMENMRAQAERFGTEIITDDVESVRLEDDIKVVTD
                     SEGTEYRARAVILAMGSAYRELGLPEEKRLSGRGVSWCATCDGFFFRDQDIAVVGGGD
                     SAVEEATFLTKFARSVTIVHRRDELRASKIMAERAHANEKISFAWNSAVDAIHGDEKL
                     TGITLRDTVTGDTRELPVTGLFVAIGHDPRNELVKGVVELDAEGYVLVEGRTTRTNIP
                     GVFACGDLVDHTYRQAITAAGSGCAAALDAERFLAH"
     gene            complement(8058..8885)
                     /locus_tag="PL014_04300"
     CDS             complement(8058..8885)
                     /locus_tag="PL014_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56343.1"
                     /translation="MTHLHDDPTPVEHDPTGMRDLLASLPDPGPMPEDLVARITAALA
                     EESRAAQASFTAKKAGRVVDAPPTTLDAGNVVPLRPHRPTLRHLGVAAAVVTAIGLGG
                     AALGAWPSSLTGPIAALSEASSADSEAGAAPAAPGADESSKGGDAEAGAGVSDLSATD
                     RLDGVTIVMSGAEHSSDALAAAASALVTTTYPTVTPLTAEAPGIGPIATAIGARTCAD
                     ALGISPDAGILVDVSEVDGAPAAVLIATDGGARTAYAVSRSCTTRSPMLIAGPIALP"
     gene            complement(8968..9642)
                     /gene="sigM"
                     /locus_tag="PL014_04305"
     CDS             complement(8968..9642)
                     /gene="sigM"
                     /locus_tag="PL014_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HOT56344.1"
                     /translation="MSADQRPGSALSGEGDAVGDPALVDAADVGELTDRELMARHVRG
                     DAGVAFGELFRRHRDRMYAVAMRTTSNRELAADAVQEAFISAFRRADAYRGEAAVTTW
                     LHRIVVNACLDRLRREKVAVRRAGDLGELDLPDPRDHHSAAEARLIVHAALAQLPEGQ
                     RLALVLVDMQGLSVAEAAVVLGVAEGTVKSRCFRGREALAELVGGPDGSRFKNLGSEA
                     EPQGAP"
     gene            complement(9659..>10698)
                     /locus_tag="PL014_04310"
     CDS             complement(9659..>10698)
                     /locus_tag="PL014_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56345.1"
                     /translation="APVDVVEPVDAEAPVDHTVALPVVAGAAAEVRGDDLSGEAEPDD
                     EGGTDLEPIEGGSPRRTASGSAAAAGAAVAGAGAAVAGALGTVGGRVGDMAKRAVDKV
                     AELSPDTAPRTSADGLEAPAPLVPAEPLSKDESKLALGIVASFLVLALVIGVWGMSRI
                     GSSTREIFGGDAAAPFPSASAASPSPSASGSAPAPAESAGPPEPLAILQVEAYDPEGD
                     GEENNKLTPKIYDGDLTTGWYSENYRTEAFGGLKKGLGVVVDLGPNKKPQTVELVIPT
                     QTSVEVYVGPENRREGATKIGEKEMAQGRVTFDVPADVSGQYVVVWFTQLSTDGDGKR
                     RAWLNEVIVTG"
     assembly_gap    10699..10798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10799..11763)
                     /locus_tag="PL014_04315"
     CDS             complement(<10799..11763)
                     /locus_tag="PL014_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56346.1"
                     /translation="MRGVGPGTVLGGRYTVHQRLEERHDTERWSAGDTTLGRRVSVLC
                     IGIDDHRTAALLDAARRAGSLSHPVFVRILDVGHDDDVAYVVEEDLEDASDLAQLVDE
                     GGVPGDEVRRITGEVAAALESARHRGMHHLDLKPEDVLRTPDGDIRLRGLETAAVRIG
                     EESTDAEDAGRRDAVGVVALAYAGLTGLWPLGAGNSGLAAAPRAPGGVATPSEVAAGV
                     PRDLDALCRLTLNEDQGPTNPGDYARQVAPWPSRQVVGRPVARMAAAPAAAAAVAAAH
                     LGAGGASAPEPVTQAESVTEIMPTIDPAAPVDVVEPVDAEAPVDHT"
     gene            complement(11856..13496)
                     /locus_tag="PL014_04320"
     CDS             complement(11856..13496)
                     /locus_tag="PL014_04320"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOT56347.1"
                     /translation="MTSQPSPSLGRSGAIMASGSLVSRAVGLVRAALLTGVVGATGYA
                     ADGFNVANTLPNQFYLLLAGGALNAILVPQIVRAKMRPNGDEFVNRIITLTLAVLAVA
                     TVTLMLAAPLLVRVYAKFDDPAALRLTTIFAVICLPQLFFYGLFTILGQVLNANGRFA
                     GFMWAPALANVVAVAGLVWFMQAGYPSGASPADWTPAMIGILAGTATLSIAIQALALV
                     IPLRRIGFRYRPVWGVRGHGLGEVSQVAKWTFGSLVVSQLGYVVTSRVLTGATERGKV
                     LGESVSGLAAYQNAFLIVMLPHGLITVSLVTALYTSLSEAASRDDTRQVRRYHDQGLR
                     LPAVIIVPGCVFLAVLAPYITSTFFFSNSLDQTRAIAVVLSVIAWMAIPMSWTYLNDR
                     VFYAHEMTWMSFRLQCVTTGVATIGAVVALQVEPARTAMALGAGQVLAFVVSAAVGFW
                     VLRRMIGNLGLRSTARTYVLLLLPAIVTAVGLLLAIGRLFPDLGETRGASGLLAGGLV
                     LGAAGAIQLGVTWGVASLLGVREISTALQPVTRRLRRR"
     gene            complement(13528..14025)
                     /locus_tag="PL014_04325"
     CDS             complement(13528..14025)
                     /locus_tag="PL014_04325"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT56348.1"
                     /translation="MSHAAADHPGMSARRLPAVEERSAGGVVVDVHEGEARIAVIARR
                     NRAGRIEWCLPKGHIEGDETLPQTAAREVAEETGIEGKVLIELGTIDYWFAAGERRIH
                     KYVHHYLLEATGGHLTIENDPDHEAIDVAWLPLRDAHRHLTFPNERRIARLAWQRLSG
                     DAASC"
     gene            14075..15574
                     /locus_tag="PL014_04330"
     CDS             14075..15574
                     /locus_tag="PL014_04330"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOT56349.1"
                     /translation="MGAGRIPLVLVPTRQPSAALLRRAVAHLAPSIPALTDLGTRFAD
                     AGHDLALVGGPVRDALLGRSVSDFDFTTSARPDETEALLAAWGTATWDMGREFGTIGA
                     RRADMVVEVTTYRADAYDGQTRKPVVAFGDSLEDDLVRRDFTVNAMALRLPDLTFVDP
                     HGGLADLEDQVLRTPSAPEVSFGDDPLRMMRAARFVAQLAFVPTPEVFAAMRELAPSI
                     EIVSAERVRDELVKLVLARHPRAGLELLTESGLAALVLPELPALRLEVDEHHRHKDVY
                     DHSMIVLEQAIDLEGPADGPPESVPGPDLVLRLAALLHDIGKPATRRFESGGGVSFHH
                     HEVVGAKLVARRMRALRFDKETTKAVARLVELHLRFHGYGDGQWTDSAVRRYVTDAGP
                     LLPRLHRLTRADCTTRNVRKARRLAATYDDLELRIERLLEHEELKAVRPELDGNEIAA
                     VLGIPPGPVLGRAYKYLLSVRLDEGVIGPEAAAERLRAWWAEQPEAAGG"
     gene            15676..17154
                     /locus_tag="PL014_04335"
     CDS             15676..17154
                     /locus_tag="PL014_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56350.1"
                     /translation="MQAAQSRRRRERGAVGLEYGAVIAIAALVVGLIAFSVSSAGADV
                     RGHVCRAVANVFQLEASCGGDGGESAEEPPEEETFEPEKCMMHESGDEYSSVIRIGFI
                     EIGENAGFVVTEYSDGSVTMMATNGAEIGATGGFGADAAWGELEAGAKVDFGANVEFD
                     YGSTWHFEDMAQAESFREQLDDYLYDQWAMTHPACGMGVCMPRPTTGAQPPPVPTTKF
                     SGVALNVDVNAALGISRTTGTAPLTQASLTEQGIASTLSGSAHWTTTTDTGGTPDDPS
                     DDTRTYVTDLSLNSEVTGQVALATGGFGSMVGMSYAVKKDSEGNIIEIQIVSTSEVTG
                     GPGGNVDGGVTQGQGQNQTGGGGSVSGGTTQGDVVVTETTLAIDPDDAASQQTVRDWM
                     GGNGDFEWPGLISLAMVDPSTADPDNPFAMLLHNNATSSTIAYDKVTDVAGFALNVKF
                     GLAFGVDFSAENSETTASEATYLGAPRPDGTRPVLDYTECVS"
     gene            17199..17750
                     /locus_tag="PL014_04340"
     CDS             17199..17750
                     /locus_tag="PL014_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56351.1"
                     /translation="MPLLRTALRRTVLAATLALTVTAGAACSGGSESSSGDAPDGWTT
                     VTDERITVQHPSDWASEPPSGKPWTHRFVGDGMEMQVAGAFSDDETASFALSKLDLPA
                     MVELDGYVGGGATAIEVEGADSALRSDFTYQVDGTPMKGVWLIAGQWPNPATAVVTIT
                     GERLDDAVIEHVVDTMTFNRRTS"
BASE COUNT         2654 a         6257 c         6227 g         2522 t
ORIGIN      
        1 cccgaggtcg tccacatggt tgtccacagt ctgtgggtat gcaaggggcg atgttcgcgg
       61 cgtgtcgtgc cgcggtgggc gccccgcacc gtgagatcct aagtcttttc cggcgtgagg
      121 tgtgcgcatc cgccggtttg acccctgcgg gcgctctcgc gtaccgtaaa caggcctgtt
      181 gttcggctgc tttcgcatgc ccgtgacgtc gcgacccaac cggtcgcagc ctcactcccg
      241 gcttcgtccc gggcttccga ttgcagccga gcgccagcgc accccgacca cccgggcact
      301 ttccggccca ccccgtagat gagagaccaa cgtgagcaag cggaccttcc agccgaacaa
      361 ccgccgtcgg gccaagaccc acggcttccg tctgcggatg cgcacgcgcg ccggccgcgc
      421 gatcctcgcc gcccgccgtc gtaagggccg ctccgagctc tccgcctgag gtcggcggtg
      481 ctgccggcgc cgaacagact gcgagagcgg tctgacttca cgtcggcggt gcgctccttc
      541 ggagggtgcc gggccgggtc caggcttctc gtggtccacg ccacctcgac cggcgcgcgt
      601 gcgggtctcc cgccgcgcgt cggtttcgtc gtgtccaagg ccgtcggcgg agccgtcgtg
      661 cgcaaccgca cgaagcgacg tctgcgggcc ctggtcgctc agcgcctgga cggcatcccc
      721 accagcgtcg acctcgtggt tcgggccaac ccgcctgcgg gacaggcgtc ctcacgggaa
      781 ctgcagcagg ctctcgaccc gttgctccgg cgagtggtca aggaactggg aggcgtggcg
      841 tgagcatcgg acgggtgctc gcgagcccgc tcatcggact gattcgcctc taccagaacg
      901 tcatctcgcc catgcggccg ccgacctgcc ggtactaccc gtcctgctcg gcctacgccg
      961 tgactgccct gcagacctac ggggtgctgc gcggttcggc gatggccacc cgccgcctgc
     1021 tgcgctgcca cccctggtcg ccgggcggtg tggaccacgt gcccctggcc gacccggtca
     1081 ccggtcggcc gatcccgggc accaccggcc ctcgggacat cggcagccac agtgctgccg
     1141 acctgcgtag ctccgatgcc gaggcggcgg cgaacgccgc gccacggcat ccgtctcacc
     1201 cccactagac ctgtcacaca cgtgacacca ccccgtcggc cttcgccggc acagcactaa
     1261 ggacatcatg ggcgacgcct tcaacgccat cctggccccg atcgagtggc tcgtggcgtg
     1321 gatcatgtac ggattccaca ccgcgctgac cgcaatcggg atgccgtccg gctcaggttg
     1381 gacgtgggcg ctgtcgatcg tcggcctcgt gctcgtcatg cgggccgcga tgatcccgct
     1441 gttcgtcaag cagatcatgg cgtcgcgcaa gatgcagatg atccagcccg agctgcagaa
     1501 gatccagaag aagtacaagg gcaagaccga cccggactcc cgtcaggcga tgacgcagga
     1561 gacgatggcg ctgtacaaga aggaggggac caaccccttc agctcgtgcc tgccgatcct
     1621 cgtgcagtcc ccgttcttct tcggcctgtt ccgcgtgctc aacggcgtcg acgagatcgc
     1681 ggcgggcacg acggacaaga tcgggccgat cacccagccg gtcgcggccg agttcgagaa
     1741 ggcgtcgatc ttcggtgccc agctctccga gaccttcctc gggtcggact cgctcaacgt
     1801 caagatcgtc accgcggtgc tcatcgtgct catgtcggcg accacgttcc tcacccagcg
     1861 ccagctgatg accaagaaca tgccggcctc ggccctggac aacccgttcg ccaagcagca
     1921 gaagctgctc ctgtacatcc tgccgctcgt gttcgccgtg tccggtgtca acttcccgat
     1981 cggtgtcctc atctactggt tcaccaccaa cgtgtggtcg atgtgccagc agttctacgt
     2041 catccggcgc atgccggccc ccggctcggc cgcggaggcg tcgtaccacg cacggttggc
     2101 caagaagggc aagccgatcc ccggtgcagc cgcggcggcg tctgccactg ctgccgcaga
     2161 ggccgaggcc atcgccgacg agaccgccaa gaagtcgggg cagcgccagc agccgaccgg
     2221 caagaagcgc aagaaggcca agggtgcggg cggcagtgcg ccggccgggg gcaagccgtc
     2281 aggcggcgcc acccccaagg acaccccgcc gagcgactcc gccaagccct ccaccagctg
     2341 attcaccccc tgaccgcagg agacacccat gagcgagaac ctcacgcagg acccggcgcg
     2401 caacgacgcc gagcccgagc agccgaccga tgtcgagtcc accagcgagc acgacgagtc
     2461 ggtgtcggac gtggtggagg ccgacgtcca ggcgatcacc ggtggtgacg tcgacgcgga
     2521 caccctcgac acggcatccg acgtcgacgt cgacgtcacg ccggcagcct ccaccagccg
     2581 ccagggccag ctcgagcgtg agggcgaggt ggccgcggac ttcctcgaga cgctgttgga
     2641 catctgcgac ctcgacggtg acctcgaggt cgacatcgac ggcgaccggg ccgccgtgtc
     2701 catcgtcgac tccgaggacg gtcgggtgcc gcggcgcctc gtgggccaga acggacaggt
     2761 gctcgacgcg ctccaggaac tcacccgcct cgcggtgcag tcggcgaccg gcgagcgcag
     2821 ccgcctgatg ctcgacgtcg gcggccaccg ggccgagcgg cgcgccagcc tggtgacgtt
     2881 ggccaaggag gccatcgagg aggtccgcac cagcggggag cgcaagccgc tcacgccgat
     2941 gaccgccttc gagcgcaagg tggtgcacga cgaggtcctc gctgccggcc tggtgtcgga
     3001 gtccgagggt gccgagccgc gccgctacgt cgtgatcctt cccgcctgag acgacgtttc
     3061 acgtgaaaca tgacgctggg gatgccgccc cgatcgcgtc cgcgccagcc gcacccgagg
     3121 gtgcggctgg cttgttcggt gaccggatgc ctctcgccga gcagttcgca gcgatcctcg
     3181 cggacaccgg tgtcagccac gggctgatcg ggccgcgcga ggtgcctcgg ctctgggagc
     3241 ggcacctgct gaactgtgcc gtcatcgcgg atgctcttcc gcgtgaggcc cgactcatcg
     3301 acgtcgggtc cggtgccggt ctgccggggg tggtgctggc catcgtgcgg cctgacctcg
     3361 aggtgcacct cgtggagccg atgctgcgtc gcacccagtg gctcgagggc gtcgtcgccg
     3421 atctggggct cgcttcggtg acggtgcacc gtggccgcgc tgaggagttc gtcggcgtgt
     3481 tggcggcgcc ctacgtcacg gcgcgggctg ttgcccggct ggacaagctg gcccgctggt
     3541 gcgttccgct gctcgagccg caaggcgtgc tcgtggcgat gaagggccgc tctgctgcca
     3601 ccgagctcga ggaggaccgc aagggcctgc aacgcctggg cctgaccgac gcggtggtca
     3661 ccgagcacgt gtcggcgctg ctcgaggagc cggtgctcac cgtcgacctg cgcttcgcac
     3721 cgaagccggc ccgcacgaag gctgcccgca gggagggtgc tcgctcctcg aagcaagcac
     3781 gagctgctcg caaggctcct tcgtagcgca gcctcatcgg tcgtgctggc caaccccggc
     3841 gcacacgcca gggtcgaggt attaccgcca gcggatccgt gcgttggcga gtcgactgcc
     3901 cgtgtccggt cgtggggcgt ctgatccggc aggctggttc tcgcggtgtt tcacgtgaaa
     3961 catgctcgct ccgagccgtc tcagacctgg ctttggcgtg ttggtgcggt gtgaggttgg
     4021 atggcgtggt tgaccactcc atggacgaag gttttgcgtt gagcacacgg atcgacagcc
     4081 cggaccatcg gaccgcggcg cccggacttg ggtttccccg gctgaccggg atgccgacag
     4141 cccccttggg gtggcccggc cccgacgccg tttcacgtga aacacctgaa ccgggcgaat
     4201 ggcccacctc ggcggagacc accgttgcgg aggtcggcgg cgccgaccag acggccgcca
     4261 ccgacggcga cgacgtttca cgtgaaacac ctgaagaacc cgtagccagc gagcctcacg
     4321 acgccgcggg agacgccgag caccgcatcg agggcgccga ggtgggggat gtttcacgtg
     4381 aaacgtcgac acccgcccgc acagcgacgg cgccgtccgg gcttccccgg gaggaggagg
     4441 tagcgcgcga gcagatcgct gcagtcctgc ccgacgccga cgcgcagaca ccactggcga
     4501 cagccgtggc gaatgacgcc cgcaagcgga tcacgttgag cggccgaacc gtgcctcgcc
     4561 ccccacgcac ccggatcctg actgtcgcca accagaaggg cggcgttggc aagaccacca
     4621 ccacggtgaa cgtcgcagca gctctgtccc aggccggcag caccgttctc gtcatcgacc
     4681 tcgaccccca gggcaacgcc agcaccgcct tggggatccc gcaccacgcc gaggtgccga
     4741 gtgtctacga cgtcctcgtc gacgggcggc cgatcagcga ggtcgtgcag gagtgcgccg
     4801 acctgccggg tctggtctgc gtccccgcga ccatcgacct cgctggcgcg gagatcgagt
     4861 tggtgtccct cgtcgcgcgc gagaccaggc tccaacgggc cgtgaccacc tacgtggagg
     4921 agcgggctgc tgctggcgac cccgtcgact acgtgctcat cgactgtcct ccgagcctgg
     4981 gtctgctcac ggtgaacgcc ttcgtcgcgg cgagtgaggt cttcatcccc atccagtgcg
     5041 agtactacgc cctcgaaggg ctctcgcagc tgctgaagaa catcgagctc atccgcaacc
     5101 acctgaaccc gagcctgcac gtctcgacga tcctgctcac gatgtacgac ggccgcaccc
     5161 gcttggccgc tcaggtggcc gacgaggtgc gcgagcactt cccgcagcag accctgcgcg
     5221 ccgtggtccc tcggtcggtg cgcatctccg aggctcccag ccacggtcag accgtcatga
     5281 cgtacgaccc gaacagcagc ggcgccctgt cctatctcga ggcagccatc gagctcgccg
     5341 agcgtgcagc cgccaccccg aacaccactg aggagtcagc atgagcgaga agcgtcgagc
     5401 cctcggccgt ggactggggg cgctgatccc gtccggcccg gccaccgacc gcccgatcga
     5461 cgtgttcttc cgtgaccagg accccgtcac gccgccttcg accgtgccgg tggacgactc
     5521 gggccagagc gcctccgaga gcgggcccca ctccggcgac ctgtcgggcg cgaacggagc
     5581 accgagcact gacagcgacg agccgacggt tgaggcgccg ccgtccgacg acggtgccgc
     5641 ggccaccgac accggcctgc gccccgttcc cggggcggaa ttcgccgaga tcccgctcga
     5701 ccacatccgc ccgaaccctc gccagccgcg cacggtcttc gacgaggacg agctggccga
     5761 gctcgtgcac tcgatccgcg agatcggcgt cctccagccg gtcgtcgtgc gacggatccc
     5821 gaccgaggcc caggacgggg aggcccgctt cgagctcatc atgggcgaac ggcgctggcg
     5881 tgcctcgcgc gaggcgggca aggacgtcgt cccggcgatc atcaaggtca ccgaggagga
     5941 cgacctcctg cgagacgccc tcctggagaa cctccaccgc agccagctca acgccctcga
     6001 ggaggccgcg gcgtatcagc agctgctcga cgacttcggc tgcacgcagg aggagctggc
     6061 tcgccggatc ggccggtcgc ggccccagat ctccaacacc ctgcgcctgc tcaagctccc
     6121 cccgctcgtg cagcgtcggg tggcggccgg ggtgctcagc gccggccacg cgagggcgct
     6181 gctcgggatg tccgactccg ccgcgatgga gcggatggca cagcggatcg tggctgaggg
     6241 tttctcggtg cgcaccgtcg aggaactcgt caccctcggt gacgacgaca cggcgccggt
     6301 ggcccggcgg gccccccggg ccggtgcccg caggccccag ctcgacgacc tcgccgccga
     6361 cctctccgac cggctcgaga cccgggtgcg catcgcgctc ggccagcgca agggccgcct
     6421 caccgtggag ttcgcctcgg tcgacgacct caaccggatc ctggacgcca tgcacctgcg
     6481 cgacccgcgg gactgagccc agccgggtca ccacacacca caagaggcgc ggatgccgtt
     6541 gtccaccaac gaaatccgcg cctcttggcg tgtgcggagc ctcagccgag gaagggctcc
     6601 agctcgcgca gcagcttcgg cttgggcatg gcgccgatga tcgtcttgac gatctcgccg
     6661 ccgacgtaga cgtgcatcgt ggggatgctc gtgatcccga gctggccggt gatggccggg
     6721 ttctcgtcgg tgttcagctt gacgatcgag acctcgtcgt ggtcgcgggc gatctcgtcg
     6781 aggatggggg cgacggcgcg gcacgggccg caccaggggg cccagaagtc gacgaggacc
     6841 ggacggtcct ggttgatgac gtcagcctcg aaggtggcgt cggaggtggc ggagacggct
     6901 cccatgggga gctcctttcg tctggggcgg ttagggctgg tggggcgggg tgaacagggg
     6961 cgggcggtcg gtgaggtggc tgatcctgcc ggagacctcc gacacggacc ggggtgaggt
     7021 cagtgcgcga ggaaccgttc ggcatccagg gcagcggcgc agccggagcc cgcggccgtg
     7081 atggcctggc ggtaggtgtg gtcgacgagg tcgccgcagg cgaagacgcc ggggatgttg
     7141 gtgcgggtgg tgcggccctc gacgaggacg tacccctcgg catccagctc gacgacaccc
     7201 ttgacgagct cgttgcgcgg gtcgtgcccg atggcgacga acagccccgt gacaggcagc
     7261 tcacgcgtgt caccagtcac ggtgtcgcgc aacgtgattc ccgtcagctt ctcgtcaccg
     7321 tggatggcgt cgacggcgct gttccacgcg aacgagatct tctcgttggc gtgggcccgc
     7381 tccgccatga tcttcgaggc gcgcagctcg tcgcggcggt gcacgatcgt caccgagcgg
     7441 gcgaacttgg tgaggaaggt ggcctcctcg accgccgagt caccgccacc cacgaccgcg
     7501 atgtcctggt cacggaagaa gaacccgtcg cacgtggcgc accaggacac cccgcgcccg
     7561 gacaggcgct tctcttcggg cagcccgagc tcgcggtagg ccgagcccat cgccaggatg
     7621 accgcgcgcg cccggtactc ggtgccctcg gagtcggtca ccaccttgat gtcgtcctcg
     7681 agccgcacgg actcgacgtc atcggtgatg atctcggtgc cgaaccgctc ggcctgcgcc
     7741 cgcatgttct ccatgaggtc cgggcccatg atgccgtcgc ggaacccggg gaagttctcg
     7801 acctcggtgg tgttcatcag ggcgccgccg gcggtgaccg cgccctcgaa catcagcggc
     7861 tcgaggttgg cccgggctgc gtagacggca gccgtccagc cagcggggcc ggagccgatg
     7921 atgatgacgt tgcggacgtc cgaggtcaca ggacacatcc ccttgtggtg gtggcgggcg
     7981 acaaccgcaa gatgcggtgc ctgttggtga accgatccta ggcccggggt gttcccgtca
     8041 ccgactcgcc ccgcgggtca gggcagggcg atcggcccgg cgatgagcat gggggagcgc
     8101 gtcgtgcacg acctggacac cgcgtaggcg gtgcgtgccc cgccatcggt cgcgatgagg
     8161 acggctgccg gggcaccgtc gacctcactc acatcgacga ggatgccggc gtcgggcgag
     8221 atccccaggg cgtcggcgca cgtgcgcgca ccgatcgcgg tggcgatcgg cccgatcccc
     8281 ggagcctccg ccgtcagtgg ggtgacggtg gggtaggtgg tggtgacgag ggccgatgcc
     8341 gcggccgcca aggcatcgga ggagtgctcg gccccgctca tgacgatggt gaccccgtcg
     8401 agacgatcag tggcggacag gtcgctcacc ccggcacctg cctcggcgtc gccccccttg
     8461 ctggactcgt cggcaccggg cgccgctgga gcggcgccgg cctcggagtc ggcagagctg
     8521 gcttcgctga gggcggcgat ggggccggtg aggctcgacg gccaggcgcc gagggcagcc
     8581 ccgcccagac cgatggccgt gaccaccgcg gccgccaccc cgagatgccg cagcgtcggg
     8641 cggtgaggtc gcagcgggac gacgttgccg gcatccagcg tggtgggggg cgcgtcgacg
     8701 acgcgacctg ccttcttggc cgtgaacgac gcctgcgcgg cccgggactc ctcggccagg
     8761 gccgccgtga tgcgggcgac gaggtcttcg ggcatgggac ccggatcggg gagtgacgcg
     8821 aggaggtcgc gcatccccgt cgggtcgtgc tcgacggggg tggggtcatc gtgcaggtgc
     8881 gtcacgagtt cctccgggtg ggtccctgct ggggggcaag actgatgtgg gcgtggcgga
     8941 cgcaggggtc gagccgggtg tgggacgtca cggtgccccc tgtggttccg cttcggagcc
     9001 aaggtttttg aatcgtgaac catccggccc ccccacgagc tcggcaaggg cctcacggcc
     9061 gcggaagcac cgcgacttca ccgttccctc ggcgacaccg agaaccacag ccgcctccgc
     9121 gaccgagagc ccctgcatgt cgacgagcac cagggccagg cgctgaccct cgggcagctg
     9181 tgccagggcc gcgtgcacga tgagccgggc ctccgccgcc gagtggtggt cacgcggatc
     9241 ggggaggtcg agctcaccga ggtcccctgc gcgccgcacg gccaccttct cgcgacgcag
     9301 gcgatccagg caggcgttga cgacgatgcg gtgcagccac gtggtgaccg cggcctcgcc
     9361 ccggtaggcg tcggcccggc ggaaggctga gatgaaggcc tcctggacgg catccgccgc
     9421 cagctcgcgg ttggaggtgg tgcgcatcgc cacggcatac atgcggtcac ggtggcgcct
     9481 gaagagctcc ccgaaggcca cgcctgcgtc accgcgcacg tggcgcgcca tgagttcgcg
     9541 gtcggtcagc tcgccgacat cggcagcatc gacgagtgcg gggtcaccga cggcatcacc
     9601 ctcacccgag agggcactgc cggggcgctg gtcagcggac atcacgaatc gaccgggctc
     9661 agccggtgac gatgacctcg ttgagccagg cccggcgctt cccatcgccg tcggtggaca
     9721 gctgggtgaa ccacaccacg acgtactggc cgctgacgtc ggcgggaacg tcgaacgtga
     9781 cgcggccctg cgccatctcc ttctcaccga tcttcgtcgc gccctcgcgg cggttctcag
     9841 ggccgacgta gacctcgacc gaggtctggg tggggatgac gagctcgacg gtctgtggct
     9901 tcttgttcgg cccgaggtcg acgaccacac ccaggccctt cttcagccca ccgaacgcct
     9961 cggtgcggta gttctcggag taccagcccg tcgtgaggtc gccgtcgtag atcttcggcg
    10021 tcagcttgtt gttctcctcg ccgtcgcctt ccgggtcgta ggcctcgacc tggaggatgg
    10081 ccagcggctc gggagggccg gcggactcgg cgggggcggg ggccgaaccg ctcgctgagg
    10141 ggctcgggga ggccgccgag gcgctcggaa aaggagctgc ggcgtcgccg ccgaagatct
    10201 cgcgcgtgct cgacccgatc cgggacattc cccagacgcc gatgacgagc gccagcacga
    10261 ggaagctcgc gacgatgccg agcgccagct tggactcgtc cttggacagc ggctctgcgg
    10321 gcaccagcgg agcgggggcc tcgagcccgt cggcactggt gcgcggtgcg gtgtcggggc
    10381 tcagctcggc caccttgtcg accgcccgct tggccatgtc gccgacgcgt cccccgacgg
    10441 tgccgagcgc gccagccacc gcagcaccgg caccggcgac ggctgccccc gccgcagcgg
    10501 cggagccgga tgccgtgcgg cgcggtgagc cgccctcgat cggttcgagg tcggtgccgc
    10561 cctcgtcgtc aggctctgcc tcaccggaca ggtcgtcacc acggacctcg gctgcggccc
    10621 cggccaccac gggtagggcc accgtgtggt ccaccggcgc ctcggcatcg acgggctcca
    10681 cgacgtccac gggtgctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
    10801 gtggtccacc ggcgcctcgg catcgacggg ctccacgacg tccacgggtg ctgcggggtc
    10861 gatggtcggc atgatctcgg tgaccgactc ggcctgcgtg accggctcag gggccgaggc
    10921 ccctcccgca ccgaggtggg cagcggcgac agcagctgcc gctgccgggg cggctgccat
    10981 tcgggccact ggccggccca cgacctgccg ggagggccac ggagccacct gtcgtgcgta
    11041 gtcgccgggg ttggtggggc cctggtcctc gttgagcgtc agccggcaga gggcatcgag
    11101 gtcgcgcggc acgccggcag cgacctccga gggggtcgcg acgccaccgg gcgcccgcgg
    11161 tgccgcggcg aggcccgagt tcccggcgcc gaggggccag aggccggtga gcccggcgta
    11221 cgcgagggcg acgacaccga cggcgtcgcg ccgaccggca tcctcggcat ccgtgctctc
    11281 ctcaccgatc cggacggcgg cggtctccag gccccgcagg cgaatgtcgc cgtccggggt
    11341 gcgcaggacg tcctcgggct tgaggtcgag gtggtgcatg ccgcggtggc gggcggactc
    11401 cagcgccgcc gcgacctcac cggtgatgcg gcgcacctcg tcaccgggca ccccgccctc
    11461 gtcgaccagc tgggcgaggt cgctggcgtc ctcgaggtcc tcctccacga cgtaggcgac
    11521 gtcgtcgtcg tgcccgacgt cgaggatccg cacgaagacc ggatggctga gcgacccggc
    11581 gcggcgagcg gcgtcgagca gggcggcggt gcggtggtca tcgatgccga tgcacagcac
    11641 cgagacgcgt cgcccgaggg tcgtgtcgcc ggctgaccac cgctcggtgt cgtgacgctc
    11701 ctccagccgc tggtgaacgg tgtagcgacc gcccaggacg gtgccgggtc caacacccct
    11761 caccgctgct cctctcgctc gtgacctcga cgtgcttcac acctcacgcc ggatctgccg
    11821 acgggtgacc tcctgccatc ctaggcacgc gggggtcagc gacgtcggag ccgacgtgtg
    11881 acgggctgga gtgccgtcga gatctcgcgc acaccgagca gactggccac cccccacgtg
    11941 acgcccagct ggatggctcc ggccgcgccc agcacgagcc cgccggccag cagcccggac
    12001 gcaccgcgcg tctcgccgag gtcggggaag agccgcccga tcgccagcaa cagccccacg
    12061 gcggtcacga tcgcggggag gagcaacagg acgtaggtgc gggccgtgga gcgcagaccg
    12121 aggttgccga tcatgcgccg cagcacccag aagcccacgg cagcggagac gacgaacgcc
    12181 agcacctgac cggcgccgag cgccatggcg gttctcgcgg gctcgacctg gagggccacc
    12241 acggcaccga tcgtggcgac gccggtcgtg acgcactgca ggcggaacga catccacgtc
    12301 atctcgtggg cgtagaagac gcggtcgttg aggtaggtcc agctcatcgg gatcgccatc
    12361 caggcgatga ccgagaggac gaccgcgatg gcccgggtct ggtcgaggct gttgctgaag
    12421 aagaacgtcg aggtgatgta gggggccaga accgccagga agacacaccc cggcacgatg
    12481 atcacggcgg gcaggcgcag cccctggtcg tggtagcggc gcacctggcg ggtgtcgtca
    12541 cgactggctg cctcggacag gctcgtgtag agcgccgtga cgagcgagac cgtgatgagg
    12601 ccgtgcggca gcatgacgat gaggaaggcg ttctggtacg ccgccagacc cgagacgctc
    12661 tcaccgagca ccttgccccg ttcggtggcc ccggtgagga cccgcgaggt cacgacgtac
    12721 cccagctggc tgacgacgag tgacccgaag gtccacttcg cgacctggct cacctcgccc
    12781 agaccgtgac cccgcacccc ccacacgggt cggtaccgga agccgatccg gcgcagcggg
    12841 atgaccaggg ccagcgcctg gatggcgatc gacagcgttg ccgttccggc gaggatgccg
    12901 atcatcgccg gggtccagtc cgccggggac gcccccgacg ggtagccggc ctgcatgaac
    12961 cacacgagac cggccacggc cacgacgttg gccagggccg gggcccacat gaagccggcg
    13021 aagcgcccgt tggcgttgag cacctgcccg aggatggtga acagtccgta gaagaagagc
    13081 tgcggcaggc agatgacggc gaagatcgtg gtcaggcgca gggcagcggg gtcgtcgaac
    13141 ttcgcgtaca cccgcacgag gaggggggcg gcgagcatca gcgtcacggt ggccacggcg
    13201 agcaccgcca gggtcaacgt gatgatccgg ttgacgaact cgtcgccatt ggggcgcatc
    13261 ttggcccgca cgatctgggg cacgaggatc gcgttgagcg cgccccccgc gaggagcagg
    13321 tagaactggt tgggcagcgt gttggcgacg ttgaacccgt cagccgcata cccggtggcg
    13381 ccgacgacgc cggtgagcag cgctgcccgc acgagcccga cggcccggct caccagcgac
    13441 ccggacgcca tgatcgcccc ggaacggccg aggctcggtg atggttggga cgtcattggt
    13501 gtcggggctc tcgtcggggg agcgggctca gcaggatgcc gcgtcgccgg acagtcgctg
    13561 ccaggcgagt cgggcgatcc gccgctcgtt ggggaaggtc aggtggcggt gggcgtcccg
    13621 caggggcagc caggcgacgt cgatcgcctc gtggtcgggg tcgttctcga tggtcaggtg
    13681 cccacccgtc gcctcgagca ggtagtggtg cacgtacttg tggatgcggc gctccccggc
    13741 agcgaaccag tagtcgatgg tgccgagctc gatgagcacc ttgccctcga tgcccgtctc
    13801 ctcggccacc tcgcgggccg cagtctgggg cagggtctcg tcgccctcga tgtgcccctt
    13861 cggcaggcac cactcgatgc ggcccgcgcg gttgcgtcgg gcgatgacgg cgatcctggc
    13921 ctcaccctcg tggacgtcga cgacgacgcc ccccgcgctg cgctcctcga ccgccgggag
    13981 ccgacgagcg gacatcccgg ggtggtcggc ggcggcgtgg ctcatccggt cactgtagcc
    14041 agtggggtca ccccgggcgg ggtacgccgc ccccatcggc gcgggccgca tacccttggt
    14101 gctcgtgccc acccgtcagc cctccgccgc gctccttcgc cgggcggtgg cgcacctcgc
    14161 gccgtccatc ccggcgctga ccgacctcgg cacccggttc gcggatgccg gtcacgacct
    14221 cgcgctcgtc ggcgggcccg tgcgcgacgc gctcctcggg cggtcggtga gcgacttcga
    14281 cttcacgacc tcggcacgac cggacgagac cgaggcgctg ctcgccgcgt ggggcaccgc
    14341 gacgtgggac atggggcgcg agttcggcac gatcggcgcc cgccgcgccg acatggtcgt
    14401 cgaggtgacg acctaccggg ccgacgccta cgacggccag acccgcaagc cggtcgtcgc
    14461 gttcggcgac tccctcgagg acgacctcgt ccggcgtgac ttcaccgtca acgccatggc
    14521 gctgcggctg cccgacctca ccttcgtcga cccgcacggc gggctcgccg acctcgagga
    14581 ccaggtgctg cgcacgccct ccgcccccga ggtgtccttc ggtgacgacc cgctgcggat
    14641 gatgcgggcc gcacgcttcg tcgcccagct cgcgttcgtg ccgacccctg aggtgttcgc
    14701 cgcgatgcgt gagctcgcgc catccatcga gatcgtgtcc gccgagcggg tgcgcgacga
    14761 gctcgtgaag ctggtcctcg cccggcatcc gcgcgccggc ctcgagctgc tcaccgagag
    14821 cgggctcgcc gcgctggtgc tgcccgagct gcccgcgctg cgcctcgagg tcgacgagca
    14881 ccaccggcac aaggacgtct acgaccactc gatgatcgtg ctcgagcagg cgatcgacct
    14941 cgaggggccg gccgacggcc cgccggagtc ggtgcccggc cccgacctcg tcctgcgcct
    15001 cgcggccctg ctccacgaca tcggcaagcc ggcgacccgg cgcttcgaga gcggcggggg
    15061 ggtctccttc caccaccacg aggtcgtcgg cgcgaagctc gtggcccgcc ggatgcgcgc
    15121 gctgcgcttc gacaaggaga ccaccaaggc ggtggcccgc ctcgtcgagc tgcacctgcg
    15181 cttccacggg tacggcgacg ggcagtggac cgactcggcc gtgcgccgtt atgtcacgga
    15241 tgccgggccg ctcctgccgc gcctgcaccg gctcacgcgc gccgactgca ccactcgcaa
    15301 cgtgcgcaag gcgcgccgcc tggccgccac ctacgacgac ctcgagctgc gcatcgagcg
    15361 actgctggag cacgaggagc tcaaggccgt gcgccccgag ctcgacggca acgagatcgc
    15421 cgcggtgctc ggcatccccc cggggcccgt tctcgggcgg gcctacaagt acctgctctc
    15481 ggtgcgcctc gacgagggcg tgatcgggcc cgaggccgcg gcggagcggc tgcgcgcctg
    15541 gtgggccgag cagcccgagg ccgccggggg ctgacccgcc ttcttgggcc ctgatcaggg
    15601 cccacggacc catcgcggat gccgggcggc gacgtagcgt cgcaggcgtc agtgaggtct
    15661 cggttggggg aaacgatgca ggcagcgcag tcgcggcgtc ggcgtgagcg gggagcggtc
    15721 ggcctcgagt acggcgccgt catcgccatc gcggccctcg tggtgggcct gatcgccttc
    15781 tccgtctcct ccgccggggc ggacgttcgc ggacacgtct gtcgggccgt ggcgaacgtg
    15841 ttccagctcg aggcgtcgtg cggtggcgac ggcggcgaga gcgccgagga gcccccggag
    15901 gaggagacgt tcgagccgga gaagtgcatg atgcacgaga gcggtgacga gtacagctcg
    15961 gtcatcagga tcggcttcat cgagatcggg gagaacgccg gcttcgtcgt caccgagtac
    16021 agcgacggca gcgtgacgat gatggcgacc aacggggcgg agatcggggc caccggcggg
    16081 ttcggggccg acgcggcctg gggagagctc gaggccgggg ccaaggtcga cttcggcgcg
    16141 aacgtcgagt tcgactacgg cagcacgtgg cacttcgagg acatggcgca ggccgagagc
    16201 ttccgggaac agctcgacga ctacctgtac gaccagtggg ccatgaccca ccccgcctgc
    16261 ggcatgggcg tgtgcatgcc acggcccacg acaggagccc agccgccgcc ggttccgacc
    16321 acgaagttct ccggggtcgc gctgaacgtc gacgtcaacg cggcgctggg catcagccgg
    16381 accaccggca ccgccccgct gacccaggcc agcctgaccg agcagggcat cgcgagcacg
    16441 ctgtcgggga gtgcccactg gacgacgacc accgacaccg gcggcacccc tgacgacccc
    16501 agcgacgaca cccgcacgta tgtcaccgac ctctcgctga actccgaggt gaccggccag
    16561 gtggcgctgg caacgggtgg gttcgggtcg atggtgggga tgtcctatgc ggtcaagaag
    16621 gacagcgagg gcaacatcat cgagatccag atcgtctcga ccagtgaggt caccggcggc
    16681 cccgggggca acgtcgacgg cggggtcacc cagggccagg gccagaacca gaccggcggc
    16741 gggggctcgg tgagcggcgg caccacgcaa ggcgacgtcg tcgtcaccga gaccaccctg
    16801 gcgatcgacc ccgacgacgc tgccagccag cagacggtgc gggactggat gggcgggaac
    16861 ggtgacttcg agtggccggg gctcatctcc ttggccatgg tcgacccgtc gaccgctgac
    16921 ccggacaacc ccttcgccat gctcctgcac aacaacgcga cgagcagcac gatcgcctac
    16981 gacaaggtca ccgacgtcgc cgggttcgcc ctcaacgtca agttcggcct ggcgttcggt
    17041 gtcgacttct ccgccgagaa ctccgagacc acggcatccg aggccaccta cctgggagcg
    17101 ccccgccctg acgggacgag gccggtcctc gactacaccg agtgcgtgtc atgatcggca
    17161 gccctcgacc caccccatcc gcgacgaaag gtcctgccgt gcccctcctg cgaaccgcgc
    17221 tgcgacgaac cgtgctcgcc gcgaccctgg cgctcaccgt caccgcgggc gccgcatgct
    17281 ccggtggcag cgagtcgtcc tccggtgacg ccccggacgg ctggacgacg gtcaccgacg
    17341 agcgcatcac cgtccagcac cccagcgact gggcctccga accgccgtcg ggcaagccgt
    17401 ggacccaccg cttcgtcggc gacggcatgg agatgcaggt cgccggcgcg ttcagcgacg
    17461 acgagacggc atccttcgcc ctgtccaagc tcgacctgcc cgccatggtc gagctggacg
    17521 ggtacgtcgg tgggggagcc accgcgatcg aggtcgaggg ggccgacagc gcgctgcgca
    17581 gtgacttcac ctaccaggtc gacggcaccc cgatgaaggg ggtgtggctc atcgccggcc
    17641 agtggcccaa cccggcgact gccgtcgtca ccatcaccgg cgagcggctc gacgacgcgg
    17701 tcatcgagca cgtcgtcgac acgatgacct tcaaccggcg cacctcgtga cccgaccgcc
//
LOCUS       DAOSOC010000032        17725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3220_length_17725_cov_9.958121, whole genome shotgun sequence.
ACCESSION   DAOSOC010000032 DAOSOC010000000
VERSION     DAOSOC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 17725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17725
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3220_length_17725_cov_9.958121"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PL014_04345"
     CDS             complement(<1..363)
                     /locus_tag="PL014_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT56352.1"
                     /translation="MVQLLNPEGERVPHPEHDAVVDALTDEELRGFYRDLVLIRRFDA
                     EATALQRQGELGLWASLLGQEAAQVGSGRALRPQDYAFPTYREHGVAWCRGVDPVNLL
                     GMFRGVNHGGWDPNEKNFH"
     assembly_gap    1368..1467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1468..2470
                     /locus_tag="PL014_04350"
     CDS             <1468..2470
                     /locus_tag="PL014_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOT56353.1"
                     /translation="WRSADGGRTWELTKLTTGQMDDWAADDPEFAARIGWTGEPAPFG
                     DSFAQVWSLACANGRVHAGTKPATLLESDDDGRTFSQVKGLTDHPSATDWGPGAAGLT
                     LHTIVPDPADPERMWVGISAAGVFTTVDGGGTWERRNDLADPADGEGHDHPAAASDGH
                     VGLCVHHVEIAAPHAGTEPADGAGTVLYQQNHHGTWRSDDGGLTWHDITAGLPSTFGF
                     PLHVHPHDAQTIWTVPLNGDSDGRYPPDAAAAVWRSCDGGASWQALRAGLPQEACFFT
                     VLRQAMSGDRRSPAGVYFGTNTGSVFASHDEGETWAEIARHLPTVLSVEVLDRAPDA"
     assembly_gap    2551..2650
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2651..3085)
                     /locus_tag="PL014_04355"
     CDS             complement(<2651..3085)
                     /locus_tag="PL014_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOT56354.1"
                     /translation="MSASTDIHGTPKRVLMLASNPSVSPTTGWPIGFWWAELTHPYWE
                     FVEAGYEVTLASPDGGTLQADSFSDPGDASGYSASDLISRGFIASPTHAAMVENTPAL
                     ADVDLASFDAVFLVGGQAPMVTFRGDQRVKDLVGTALDAGKVV"
     gene            complement(3814..4902)
                     /gene="hisC"
                     /locus_tag="PL014_04360"
     CDS             complement(3814..4902)
                     /gene="hisC"
                     /locus_tag="PL014_04360"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT56355.1"
                     /translation="MSEDVVTLRSALAKLPAYTPGKPASAPPGVTAYKISSNENPFPP
                     LPSVLDAVQAAAGEMNRYPDMGVTELTATLAARLDVPPDRLAFGPGSVGVLGQLIAAT
                     CDAGDEVVFAWRSFEAYPILVTLAGAVPVQVPLGPGDRHDLEAMADAITERTRLVLVC
                     TPNNPTGTTVTDAELELFLERVPRDVVVVIDEAYLEFVTAPDAPDAVAVHRSRPNVVV
                     LRTFSKAYGLAGLRVGYAVAHEPVALALRQAAIPFGVNSLAQVAAVASLTASDELEVR
                     VKELVAERERVLAALAEQGWELSDSQANFVWFGLGEDTAAFAAACQDAGLTVRPYGTD
                     GARATIAEREANDRLIEVAGAFLAARRT"
     gene            5000..5386
                     /locus_tag="PL014_04365"
     CDS             5000..5386
                     /locus_tag="PL014_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOT56356.1"
                     /translation="MKNFLIRVGVNGVALWVAALVLPGINLAEGDVGWGRKLWTIFFV
                     AVIFGVVNAIIKPIAQLLSLPFIVLTLGLFIFIVNAFMLQLTEWVSGWLDLSFSIENF
                     FWDAIFGAIIITLVSWVLNVVLPEDD"
     gene            5415..6434
                     /gene="galE"
                     /locus_tag="PL014_04370"
     CDS             5415..6434
                     /gene="galE"
                     /locus_tag="PL014_04370"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOT56357.1"
                     /translation="MRVLVSGGAGYIGSHTVVQLVAAGHDVVVVDSFANAKPAVVGRL
                     EALTGKPLEVHSFDLTDHDKTEHLFANDRIDAVIHFAGFKAVGESVAKPLEYYENNLG
                     STFSLVRAMRRHGVTKLVFSSSATVYGADAPVPMREDLPTSATNPYGWTKVMQEQILR
                     DVAFSDPTWRIALLRYFNPVGAHASGTIGEDPSDIPNNLMPYLAQVAVGRREKLSVFG
                     GDYPTPDGTGVRDYIHVEDLAAGHIAALTRLDAVDDAVSTWNLGTGHGTSVLELVAAF
                     SRACGHDLPYEIVDRRPGDVAASYADATRAEAELGWRATRTVDDMCVDTWRWQSQNPH
                     GYPDA"
     gene            6601..8055
                     /locus_tag="PL014_04375"
     CDS             6601..8055
                     /locus_tag="PL014_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOT56358.1"
                     /translation="MDAVDVARWQFAITTVYHFLFVPITIGMSLIVAIFHTQWLRTRN
                     PEYLRLTKFYGKLFIINFALGLVTGIVQEFQFGMNWSDYSRFVGDIFGAPLAFEALLA
                     FFLESTFIGIWVFGWGRIPEKLHAACMWLVHIGTVLSAYFILAANSFMQNPVGYRFNP
                     ETGRAEMADFMAVLTNKVQLVTFPHVIFSAYMVGGAVVMGVGLWLMRKHASTGDEPMY
                     RKATRFGAVFALVASLGVIVTGDIQGKIMTEVQPMKMAAAEGLYETTSNAPFSVLSIG
                     SLDGSEATRIIEIPGLLSFLATGTWDGEVEGINELRVSERAMAEKVAEQYGPEVAALV
                     LVDDYTPMIPLAYWSFRFMMGLGFATMAAAAATLWAMRKNRVPQGSVWTWVAILTPLA
                     PVFANSWGWIFTEAGRQPWIVYGLMTTSTGVSPSVSATEVWISMIAYTLVYAVLAVIE
                     IKLFLTYVRRGADPFEDPADHADADEDAPLQFAY"
     gene            8078..9106
                     /gene="cydB"
                     /locus_tag="PL014_04380"
     CDS             8078..9106
                     /gene="cydB"
                     /locus_tag="PL014_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOT56359.1"
                     /translation="MDYALIWFGLIGLLWAGYLVLEGFDFGVGALLPVLGKGTSDADS
                     EKRRRVMLNTIGPHWDGNEVWLLTAGGATFAAFPEWYATMFSAFYLPLLILLVALIVR
                     NMGFEYRGKRDDDTWRARWDMAIIGGSIVAPLLVGVALTNVVRGVPLDADSEFVGNLF
                     TLLNPMSLLGGLVVLGLSLTHGAFFLALKTTGDIRRDARALGTTLGLVTAGLAVVLLL
                     WLGLTQGTVWSWITTAIAAVSLLAAIYANVKESEGWAFIGTAVTFASAVATYFLTLYP
                     NVMPTTLADGTSLTLENASSSEMTLKIMTVAALVFTPIVLLYTAWTYWTFRKRLGTHH
                     MPAPVSVK"
     gene            9121..10707
                     /gene="cydD"
                     /locus_tag="PL014_04385"
     CDS             9121..10707
                     /gene="cydD"
                     /locus_tag="PL014_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HOT56360.1"
                     /translation="MRPFDPRLLRAAPAARRPVAVLAVVGVLQGIATIGLAVALTALV
                     VAVVEGMPLRPPASWLAGLFVARAGLSWVAEKVAAWAGVEVTAQLREALLARWLASPA
                     ERRPDPDRAVTLAAQGAASVEPYAARFLPALVAGAVVPALALATLVWVDWISALIVVL
                     TLPLLPFFAALIGKTTQADTEKRWAALSSLSGHFLDVVRGLPTLVTYGRAQRQVEVIG
                     EVSQQHRRATMATLKLAFMSSAALELLASISVAIVAVSVGIRLTHGSMTLQAGLLAIL
                     LAPEAYWPVRRVGAEFHAAADGAEAIDGILAELDPATASPEAPRPGDELGVVLDGIHY
                     TYPESADAVLAGVTLDAGPGLTAVTGPSGVGKSTLLELAAGLRTPTAGTVRAGRAHLV
                     TQRPFLPAGTLREALALGNDADDQALWDALRLVGLEGFVAGLPLALATPLGDDGFGLS
                     AGQRARIALARATLSTAPVLLVDEPTAHLDEAAATLVHDVLAGLGERRTVIAVTHRSE
                     LVARADRHVALTRDGAEVLA"
     gene            10704..12383
                     /gene="cydC"
                     /locus_tag="PL014_04390"
     CDS             10704..12383
                     /gene="cydC"
                     /locus_tag="PL014_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HOT56361.1"
                     /translation="MSLRPTRATIIGGLLGGAATASGIALTATSGWLIVRASEQPVIL
                     TLLTAIVGVRAFGIARPLFRYVERLVSHDAALDDLARERTRVYSALVPLTPARLGRRS
                     RSRVLAGVVDDLTDVVDAQVRVTVPVLSSIVAGLVATLLVAWFSPAVGLVLAGLLVAV
                     AATCWLAWLLESRSRDELLEARAEVLRVSDLVARQAGELQAIGAQDTASGWLRDAHDA
                     LRGATRQQSRGRALVAATLLVATAAATIAAAFLADPAATGAPVAALLVVVPVAVGDAL
                     APLVDSMRALARAQGSAARLEALLDQEPAVSDPVSAPATPADVRQVGASSSQGAGRNA
                     VTGRHVSLEQVAASWTGTGDQLAPTDLTLEPGQRVAVVGPNGSGKSTLLAVLARHLDP
                     SRGRHTVDGVDVRDLPLDEVRRHVAVVDDEPHVFASSVAANVRLAAPEASDDGIRTAL
                     GRAGLGAWVDALPEGLETRLGTGGRGVSGGERTRLGVARALLADRPLVLLDEPTAHLD
                     HATATAVLDDVLDATDDRAVVLVSHRPEAAERFHRVLDLGVASKAATTLKE"
     gene            12386..12844
                     /locus_tag="PL014_04395"
     CDS             12386..12844
                     /locus_tag="PL014_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOT56362.1"
                     /translation="MPHSTSAPRLGDLERVVMEHLWEAASSGGADFEGATVRQVHDRF
                     EGEREIAYTTVMTVLDRLSKKSLVTRERDGRAWRYLPADTREALTAQTMRRTMTDMDL
                     TDRRAALLHFLDGATTDEIADLKAALAEIEGRAADAASPPAARRRGLRRP"
     gene            12966..13829
                     /locus_tag="PL014_04400"
     CDS             12966..13829
                     /locus_tag="PL014_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOT56363.1"
                     /translation="MIPLALATLALLLAWVAPGLMARQKQFRRAPRASLVAWQAVTLG
                     GVVAAVAVPLSALPLVMDRGEPLARAWVLVPAVLASTAIVARLLYAGHDVGTELRRVR
                     QDHRQLVDIIAGHDGEHAQLRILEHPTPTAYCIPGRHSRVVLSQGVLDSLPPAELSAV
                     LAHEQAHLRGRHDLLLEFFTVVHHAVPSRLRSAAALAEVRLLVEALADRAAVRRSGEV
                     ATARALMALAGSRTPAGETGATLGVGTTAPVRLRLLVDGPPHPALPAVAYLYAAGSAT
                     LPVALLVAAWL"
     gene            complement(13836..14282)
                     /locus_tag="PL014_04405"
     CDS             complement(13836..14282)
                     /locus_tag="PL014_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56364.1"
                     /translation="MSFFERAKAAANDLAAKADAEMRKAGISTPGGVPGTGGFGGGGA
                     ASAAGDRALRDLGVLTYLEATGRPSSAQDRDRVLAALRGLESSGQLGSLTLSAAPPPP
                     PGAQAGPPPPPGAAAAAAGSAPPPPPPPPPSDAGSPPPPPPPPSWA"
     gene            14409..15134
                     /locus_tag="PL014_04410"
     CDS             14409..15134
                     /locus_tag="PL014_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOT56365.1"
                     /translation="MTIHGSLFHEFKEKETQDPFALQNKKLLRIDMRYGEVWARTGSM
                     VAYQGDVRFVNKGSGGFGKMLKAAATGEGVKMMQCSGSGELFVADEASEIQIMYLEND
                     ALSVNGANVLAFSNSIQWDIHRIQARGATMTGGLYNVSLRGTGYVAVTTKGDPIALDV
                     ASSPTFADAQAVVLWTSGVSMDIRVDTGGLGSMIRGGTGELLQMAFGGQGYVLVQPSE
                     SVPQGGHQSGGQTPGGMLGQFMQ"
     gene            15225..16238
                     /gene="bioB"
                     /locus_tag="PL014_04415"
     CDS             15225..16238
                     /gene="bioB"
                     /locus_tag="PL014_04415"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOT56366.1"
                     /translation="MTAQVTGTDILDVAREQVLERGVPLTQGQIEQVLRTGDDQLQAL
                     LALAHEVRLKYQGPSVEVEGIVSLKTGGCPEDCHFCSQSGQFTSPVRSVWLNIPELVR
                     AAKATAATGASEFCIVAAVRGPDDKLMEQMREGVAAIRAAVDINVAASLGMLSREQVA
                     DLVDMGVHRYNHNLEAGRSYFPQVVTTHSFEERWDTCLMVKESGMELCCGGLVGMGET
                     LEQRAELAAQLSELEPHEVPLNFLNPRPGTPFGDLEPMETGDALRTIAAFRLALPRTI
                     LRYAGGRELTLGDLGTREGLLGGINAVIVGNYLTTLGRDPREDLALLDELEMPIKALN
                     DTF"
     gene            16809..17381
                     /locus_tag="PL014_04420"
     CDS             16809..17381
                     /locus_tag="PL014_04420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT56367.1"
                     /translation="MSLHVLVLVGSLRAGSTNAQLADAAIAHLPAGVDGTVFARLAEL
                     PHYSEDLDHDDSLPEVARDLREAVADADAVLLVSPEYNGSLPGVLKNAVDWVSRPRGA
                     AAIAGKPAAVIGASGSPRGAQWAREDGIKVLKVAGAAPFEDTVGVGSAFQAFHDGRLL
                     DSELDGALRTLVTELVDAAQRTSDSDRPAA"
     gene            17474..>17725
                     /locus_tag="PL014_04425"
     CDS             17474..>17725
                     /locus_tag="PL014_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     aminotransferase BioA"
                     /protein_id="HOT56368.1"
                     /translation="MTATPAPPPRGPDAGSVEELLAFDRDHLWHPYSSALTPQDPYLV
                     ESAHGIRLRLRDRAGESREVIDAMSSWWCAIHGYAVPELD"
BASE COUNT         2417 a         6402 c         6107 g         2599 t
ORIGIN      
        1 gtggaagttc ttctcgttgg ggtcccagcc gccgtggttg accccgcgga acatgccgag
       61 caggttgacc gggtcgacgc cgcggcacca cgcgacgccg tgctcgcggt aggtcgggaa
      121 ggcgtagtcc tgggggcgca gcgcgcggcc ggagccgacc tgggcggcct cctgcccgag
      181 caggctcgcc cacaggccca gctcgccctg ccgctggagc gcggtggcct cggcgtcgaa
      241 gcgccggatc agcacgaggt cgcggtagaa cccgcgcagc tcctcgtcgg tcagggcgtc
      301 gacgacggcg tcgtgctcgg ggtgcgggac ccgttcccct tcgggattga gcagctgcac
      361 catctcgggg ccaccgtcct gcacgaggcg gacggggttc tcgggggcgg tcgcgcggct
      421 gacgtggtgg tccacgccga acgcggtctc gaggggggcg acgtcgctgt cgctcacagg
      481 gcactccttg tccgggcgcc cgtggtgacg gatgccgcgt cgagccgacc gtaggcgtcg
      541 ccgggtgtgc gtcgtcgcgg tcgacagggg ttgggtggcc gcgatgctgg gtgtggctgc
      601 ggtcactgcc gatgtcggga ccaccgtacc ggtgacatgt ggccgcagag aaccacatgg
      661 ggcgagcagt gcgtggcccc gagtgcctgc gtcagcacca ccaggcgttc ggcgtcagga
      721 ccagggttgc gatgacgcca ccgcccggcc cctggtccgc agcgaagaga ttcgcctcga
      781 cacgacgccc accgaacacg gccccacggc ttcggtcacc cgtgcgccgg tggcggcggt
      841 gaaccacgcc gtgtctgccc gtgccgcact ccacgccgcc acatcgccgg ctctgaccct
      901 gtcgtccact ccgaaaagtc accgaggccg atcaccacca ccaccaccac cactgacaac
      961 cgcgcaacgc tcttcacaac tcccccctcc ggccgtcagc ggtcacgtgc cattccagca
     1021 cccaccaccc accaccgccg cggccgcaac tggggtggac ccaccaaccg ggattgcgtt
     1081 gtcccccgcc atcgccgcgc atggatcgcg ctgactgacc tgcttgaccg atccatttgc
     1141 cggttggcgg gcccggccag gcgcagccga ggctggaatc tgagagagca gggtgggggt
     1201 gcctgtgggg gtcgttgccc gccgcaagga ctcccttcac gagcgcaagg ggtcgtgggc
     1261 gcaccccgtg cggccggcgc accgagcgag caggtggcag caagctcgtc cgtcggcgcg
     1321 cgggcagcat tccgtgcccg tcaccttgcc ggatgccggg tggctggnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnngtg gcgctcggcc gacggcggcc ggacctggga
     1501 gctcacgaag ctgacgaccg ggcagatgga cgactgggca gcggatgacc ctgagttcgc
     1561 ggccaggatc ggctggaccg gggagcccgc acccttcggc gactcgttcg cgcaggtgtg
     1621 gtcgctggcg tgcgccaacg gccgcgtcca cgcaggtacc aagccggcga ccctgctcga
     1681 gagcgatgac gacggccgga cgttctcgca ggtcaagggc ctgaccgacc acccgtcagc
     1741 aaccgactgg ggcccgggag cggccgggct gacgctgcac acgatcgttc ccgacccggc
     1801 cgacccggag cgcatgtggg tcggcatctc cgccgccggg gtgttcacca cggtcgacgg
     1861 tgggggcacg tgggagcggc gcaacgacct cgccgaccct gccgacggtg agggacacga
     1921 ccaccctgcc gcggcatccg acgggcacgt gggcctctgc gtgcaccacg tcgagatcgc
     1981 cgctccccac gcaggcaccg aacctgccga cggggccggc accgtgctgt accagcagaa
     2041 ccaccacgga acctggcgct ccgacgacgg cggcctcacg tggcacgaca tcacggccgg
     2101 actgccctcg accttcggct tcccacttca cgtgcacccg cacgacgccc agaccatctg
     2161 gacggtcccg ctcaacggcg acagcgacgg ccgctatcca ccggatgccg cggcggccgt
     2221 ctggcgatcg tgcgacggtg gtgcgtcgtg gcaggcgctg cgggcggggc tgccgcagga
     2281 ggcgtgcttc ttcaccgtcc tgcgacaggc gatgagcggt gaccggcgct ccccggccgg
     2341 ggtgtacttc gggaccaaca ccgggtcggt cttcgccagc cacgacgagg gcgagacctg
     2401 ggccgagatc gcccggcacc tgccgaccgt gctgtcggtg gaggtcttgg accgggcccc
     2461 cgacgcctga gcgcgccgcc gcgcagacag cggcgcgctc agggtgcccc acccggagca
     2521 gatcagtcgc gagccgagcc tgtgcgcagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn gacgaccttg cctgcatcca gcgcggtgcc gacgaggtcc ttgacgcgct
     2701 ggtcgccccg gaaggtcacc atcggcgcct gcccgccgac gaggaagacc gcgtcgaagg
     2761 aggcgaggtc gacatcggcg agggcggggg tgttctcgac catcgccgcg tgggtggggc
     2821 tggcgatgaa cccccgggag atgagatcgc tggccgagta gccggaggcg tcgccggggt
     2881 cggagaacga gtcggcctgc agggtgccac cgtccgggct ggcgagggtg acctcgtagc
     2941 cggcctcgac gaactcccag taggggtggg tcagctcggc ccaccagaac ccgatcggcc
     3001 agccggtcgt cggggagacc gacgggttgg acgcgagcat gaggacgcgc ttgggcgtgc
     3061 cgtggatgtc ggtggatgcg ctcatgggtc ctggtactcg gtgcacatcg cgggccgccc
     3121 ggtgcccgac aactgggcct cgcgggtcgc gaccctggtg tggacggcct gcggagggca
     3181 accgcccgcg tgagcactgc gggccgagcc tgagcgctgc ctggcggacc caccaactgg
     3241 caatgccgac gtgcggccct gcgccgctgg acgaccaatg ccgcggagcg cggcgccagc
     3301 tgagcacgaa tcgcacgctc aacggcatcc tgccggggct tttgcacgtc cggcggcgca
     3361 gccaggatgc cgcccgcccg gattcgcgcg atgtggagat ggccctttgc cggttggcag
     3421 gcccgcgcac ctgaggtggg gaacggctga gggcagcaca cgggggaatc cgcacacagg
     3481 ggaccgcaaa ctggagtctg agagtgcaga ggttaggggt gcctgtggga gtcgttgccc
     3541 gccgcaagga cttccttcac gggggcaatg actcgtacag gtaccccctc tggtcggcgc
     3601 gcccgaccag cacgggtgga ccgggcaccc cagcgctgcc ttgatcgggg cccctgcgcc
     3661 gctggacgac caatggcgcg cagcgcggcg ccagttgagc acgaatggca ggctcagcag
     3721 cgcccagccc ggccttctgt acgtccggtg gcgcgaacag gatgccgggc ggggggtgac
     3781 gctcagacgt cggggtggcg gtgggggtgg cgctcaggtg cggcgggcgg cgaggaaggc
     3841 cccggccacc tcgatgagcc ggtcgttggc ctcacgctcg gcgatcgtgg cgcgggcccc
     3901 gtcggtgccg tacgggcgca ccgtcagccc ggcatcctga caggccgcgg cgaaggccgc
     3961 cgtgtcctca ccgagcccga accacacgaa gttcgcctgc gagtccgaga gctcccaccc
     4021 ctgctcggcc agcgccgcca gcacccgttc gcgttcggcc accagttcct tgacccgcac
     4081 ctcgagttcg tcggatgccg tgagcgacgc caccgcggcg acctgcgcca acgagttgac
     4141 cccgaacggg atcgcggcct gccgcagcgc cagggccacg ggctcgtggg ccacggcata
     4201 gccgacccgg agcccggcga ggccgtaggc cttggagaag gtgcgcagga cgacgacgtt
     4261 gggccgcgaa cggtggaccg cgacggcatc cggggcatcg ggcgcggtga cgaactcgag
     4321 gtacgcctcg tcgatcacca cgacgacgtc ccgcggcacc cgctcgagga acagctcgag
     4381 ctcggcgtcg gtgaccgtgg tgccggtcgg attgttcggc gtgcagacga gcacgaggcg
     4441 ggtgcgctcg gtgatcgcgt cggccatcgc ctcgaggtcg tgccggtcgc cggggcccag
     4501 cggcacctgc accggcaccg ccccggccaa ggtcaccagg atgggatacg cctcgaagga
     4561 gcgccacgcg aagacgacct cgtcgccggc gtcgcaggtc gccgcgatga gctgaccgag
     4621 cacaccgacc gaacccgggc cgaaggccag ccgatcgggg ggcacgtcga ggcgggccgc
     4681 gagcgtcgcg gtcagctcgg tgacgcccat gtcggggtag cggttcatct cgcccgcggc
     4741 agcctgcacg gcgtcgagca ccgagggcag cggcgggaac gggttctcgt tgctgctgat
     4801 cttgtaggcg gtgactcccg gcggggcgga ggcgggcttg cccggggtgt aggccggcag
     4861 cttggccagg gcggatcgca gggtcacgac gtcctcactc atgacagcca ctgtaggccg
     4921 ggcggacgag cggccacccg ccgcggcgag ttgacgccga aaccggccca cgcggcatcc
     4981 ggcctgtcag actgccagcg tgaagaactt cctgattcgt gtcggtgtca acggtgtcgc
     5041 cctgtgggtt gccgcgctgg tcctgccggg catcaacctc gcggaaggtg acgtcggctg
     5101 gggccgcaag ctctggacga tcttcttcgt ggccgtgatc ttcggcgtcg tcaacgccat
     5161 catcaagccg atcgcccagt tgctgtcgct gccgttcatc gtgctgacgc tcgggttgtt
     5221 catcttcatc gtcaacgcgt tcatgctgca gctgaccgag tgggtcagcg gctggctcga
     5281 cctgtcgttc agcatcgaga acttcttctg ggacgcgatc ttcggcgcca tcatcatcac
     5341 gctcgtgtcg tgggtgctga acgtcgtcct tcccgaagac gactgaaccc ggcgaccccg
     5401 ataggttggg cgccatgcgc gtcctcgtct ccggcggtgc cggctacatc gggtcccaca
     5461 cggtcgtcca gctcgtcgcg gcgggccacg acgtcgtcgt cgtcgactcc ttcgccaacg
     5521 ccaaacccgc ggtggtcggc cggctcgagg ccttgaccgg caagccgctc gaggtgcact
     5581 cgttcgacct caccgaccac gacaagaccg agcacctgtt cgcgaacgac cggatcgacg
     5641 ccgtcatcca cttcgcgggg ttcaaggcgg tcggtgagag cgtggccaag ccgctggagt
     5701 actacgagaa caacctcggc agcacgttct cgctggtgcg cgccatgcgc cggcacgggg
     5761 tgaccaagct ggtcttctcg tcctcggcca cggtctacgg cgccgacgcc cccgtgccga
     5821 tgcgtgagga cctgccgacc tcggcgacca acccgtacgg ctggaccaag gtcatgcagg
     5881 agcagatcct gcgcgacgtc gccttctcgg acccgacgtg gcggatcgcc ctgctgcgct
     5941 acttcaaccc ggtcggggca cacgcgtcgg ggaccatcgg cgaggacccg tccgacatcc
     6001 cgaacaacct catgccctac ctggcccagg tggccgtagg gcgccgcgag aagctgtcgg
     6061 tcttcggcgg cgactacccg acacccgacg gcacgggggt gcgcgactac atccacgtcg
     6121 aggacctcgc cgccgggcac atcgcggcgc tcacgaggct ggatgccgtg gacgacgccg
     6181 tctcgacgtg gaacctcggg accggccacg gcacatcagt gctcgagctc gtcgcggcct
     6241 tctcgcgggc gtgcggtcac gacctgccct acgagatcgt cgaccgccgc cccggtgacg
     6301 tggctgcgtc gtacgccgac gccacccgcg ccgaggccga gctcggctgg cgcgcgaccc
     6361 gcaccgtgga cgacatgtgc gtcgacacct ggcgctggca gtcgcagaac ccgcacggct
     6421 accccgacgc ctgaccggca ccagcgccca cggggcgtac ccagatcgac caataggtca
     6481 ctgggggtac aggtgtgcgc ggcttcacat cgtcgaaggc tggacagagg tcccggccct
     6541 ctcctacacg gagtagtttt tgacctacta cgtcttgtcg tttgacttgc aggagccttc
     6601 atggatgccg tcgacgtcgc caggtggcag ttcgccatca cgaccgtgta ccacttcttg
     6661 ttcgtgccga tcacgatcgg catgtcgctc atcgtggcga tcttccacac gcagtggctg
     6721 cgcacgcgca accccgagta cctgcgactg accaagttct acggcaagct cttcatcatc
     6781 aacttcgccc tcgggctggt caccggcatc gtgcaggagt tccagttcgg gatgaactgg
     6841 tcggactact cccgcttcgt cggtgacatc ttcggcgccc cgctggcgtt cgaggcgctg
     6901 ctcgccttct tcctggagtc gaccttcatc ggcatctggg tgttcggctg gggccggatc
     6961 cccgagaagc tgcacgccgc ctgcatgtgg ctcgtgcaca tcggcaccgt gctgtcggcc
     7021 tacttcatcc tcgcggcgaa ctcgttcatg cagaaccccg tcggctacag gttcaacccc
     7081 gagacgggcc gcgccgagat ggccgacttc atggccgtgc tcaccaacaa ggtgcagctc
     7141 gtgaccttcc cgcacgtcat cttctcggcg tacatggtcg gcggcgccgt cgtcatgggc
     7201 gtcggcctgt ggctgatgcg caagcacgcc agcaccggtg acgagccgat gtaccgcaag
     7261 gccacccgct tcggcgccgt cttcgccctg gtcgcgtcgc tgggcgtcat cgtcaccggt
     7321 gacatccagg gcaagatcat gaccgaggtc cagcccatga agatggccgc ggccgagggc
     7381 ctgtacgaga cgacgtcgaa cgccccgttc tcggtgctgt cgatcggctc gctcgacggc
     7441 agcgaggcca cccggatcat cgagatcccg ggcctgctct ccttcctcgc gaccggcacc
     7501 tgggacggcg aggtcgaggg catcaacgag ctgcgcgtca gcgagcgcgc gatggccgag
     7561 aaggtcgccg agcagtacgg ccccgaggtc gccgcgctcg tgctcgtcga cgactacacc
     7621 ccgatgatcc cgctcgccta ctggtcgttc cgcttcatga tgggcctggg cttcgccacg
     7681 atggccgcag ccgccgcgac cctgtgggcg atgcgcaaga accgggtccc gcagggctcg
     7741 gtctggacgt gggtcgcgat cctcacaccg ctcgcacccg tcttcgccaa cagctggggc
     7801 tggatcttca ccgaggccgg ccgtcagccg tggatcgtct acgggctgat gaccacctcg
     7861 accggggtct ccccctcggt cagcgccacc gaggtctgga tctcgatgat cgcctacacc
     7921 ctcgtgtacg ccgtgctggc cgtcatcgag atcaagctct tcctcaccta cgtccgccgc
     7981 ggcgccgacc ccttcgagga cccggccgac cacgcggatg cggacgagga cgccccgctc
     8041 cagttcgcct actgatcgac gtcaggactg aaacgccatg gactacgcac tcatctggtt
     8101 cggactcatc ggtctgctgt gggccggcta cctcgtgctc gagggcttcg acttcggtgt
     8161 cggcgccctg ctgcccgtgc tcggcaaggg caccagcgac gccgactccg agaagcgccg
     8221 ccgcgtcatg ctcaacacca tcggccccca ctgggacggc aacgaggtct ggctgctcac
     8281 cgccggtggc gccaccttcg cggccttccc cgagtggtac gcgacgatgt tctcggcgtt
     8341 ctacctcccg ctgctcatcc tgctcgtcgc cctcatcgtg cggaacatgg gctttgagta
     8401 ccgcggcaag cgcgacgacg acacctggcg cgcgcgctgg gacatggcga tcatcggcgg
     8461 ctccatcgtg gcgcccctgc tcgtcggcgt cgccctgacc aacgtcgtgc gcggtgtgcc
     8521 gctcgatgcc gacagcgagt tcgtcggcaa cctgttcacc ctgctcaacc ccatgagcct
     8581 gctcggtggc ctcgtcgtgc tcggcctcag cctcacccac ggcgcgttct tcctcgccct
     8641 gaagaccacg ggcgacatcc gccgcgacgc ccgggccctg ggcaccaccc tcgggctggt
     8701 caccgccggc ctcgcggtcg tgctgctgct ctggctgggc ctgacccagg gcacggtctg
     8761 gtcgtggatc accaccgcca tcgccgcggt gtcgctgctc gccgcgatct acgccaacgt
     8821 caaggagagc gagggctggg ccttcatcgg caccgccgtc accttcgcca gcgccgtcgc
     8881 cacgtacttc ctgacgctct acccgaacgt catgccgacg accctggccg acggcacgtc
     8941 gctcaccctc gagaacgcct cgagcagcga gatgacgctg aagatcatga ccgtggccgc
     9001 cctggtcttc acgccgatcg tgctgctcta cacggcgtgg acgtactgga cgttccgcaa
     9061 gcgcctgggc acccaccaca tgccggcacc ggtctcggtc aagtaacgcc acggcatccg
     9121 atgaggccct tcgacccacg actgctccgg gcggcgccag cagcgcgccg cccggtggcg
     9181 gttctggccg tcgtgggcgt gctccagggc atcgcgacga tcgggctggc ggtggcgctc
     9241 accgcgctcg tcgtcgccgt cgtcgagggg atgccgttgc gtccgccggc gtcgtggctg
     9301 gcgggtttgt tcgtggcgcg ggccgggctg tcctgggtcg cggagaaggt cgcggcctgg
     9361 gccggggtcg aggtcaccgc gcagctgcgc gaggccctgc tcgcccgctg gctggcctcg
     9421 ccggcggagc gccgccccga ccccgaccgg gccgtgaccc tggccgcaca gggcgccgcg
     9481 agcgtcgagc cgtatgccgc gcgcttcctg cccgcgctcg tcgccggagc ggtggtaccg
     9541 gcgctggccc tcgccacctt ggtgtgggtc gactggatca gcgcgctcat cgtcgtgctg
     9601 accctgcccc tgctgccgtt cttcgcggcg ctcatcggca agaccaccca ggccgacacc
     9661 gagaagcgct gggcggccct gtcgtcgctg tccgggcact tcctcgacgt cgtgcgcggc
     9721 ctgccgaccc tggtcaccta cggccgggcg cagcgccagg tcgaggtcat cggcgaggtc
     9781 agccagcagc accggcgcgc cacgatggcc accctgaagc tggcgttcat gtcgtcggcg
     9841 gcgctcgagc tgctcgcctc gatctcggtg gcgatcgtcg cggtcagcgt cggcatccgc
     9901 ctcacccacg ggtcgatgac cttgcaggcg gggctgctcg cgatcctgct cgcccccgag
     9961 gcgtactggc cggtgcgccg cgtcggtgcc gagttccacg ccgcggccga cggcgccgag
    10021 gcgatcgacg gcatcctcgc cgagctcgac cccgccaccg catcgcccga ggcaccgcgc
    10081 ccgggcgacg agctgggcgt ggtgctcgac ggcatccact acacgtaccc ggagagcgcg
    10141 gatgccgtgc tcgccggggt cacgctggat gccgggcccg gcctgaccgc cgtcaccgga
    10201 ccctcggggg tcggcaagtc caccctcctc gagctcgcgg ccgggctgcg cacgcccacg
    10261 gccggtaccg tgcgcgccgg tcgcgcccac ctcgtcaccc agcggccgtt cctgcccgcc
    10321 ggcaccctgc gcgaggccct cgcgctgggc aacgacgccg acgaccaggc cctctgggac
    10381 gccctgcgcc tcgtcggcct cgaaggattc gtcgccggcc tgccgctcgc gctcgcgacc
    10441 ccgctcggcg acgacgggtt cgggctgtcc gcagggcagc gggcccgcat cgccctggcc
    10501 cgggccaccc tgtccaccgc gccggtgctg ctcgtcgacg agcccaccgc gcacctcgac
    10561 gaggccgccg ccacgctcgt gcacgacgtg ctggccggcc tcggcgagcg gcgcaccgtc
    10621 atcgccgtga cgcaccgctc cgagctcgtc gcccgggccg accggcacgt cgccctgacc
    10681 cgcgacggtg cggaggttct ggcatgagcc ttcgaccgac acgggccacg atcatcggcg
    10741 ggctgctggg aggtgccgcg acggcatccg gcatcgccct gacggcgacc tcgggctggc
    10801 tcatcgtgcg agccagcgag caaccggtga tcctcaccct gctgaccgcc atcgtcgggg
    10861 tgcgggcctt cggcatcgcc cggccgctgt tccggtacgt cgagcggctc gtgtcgcacg
    10921 acgcggcgct cgacgacctc gcccgcgagc gcacccgcgt ctactcggcg ctcgtgccgt
    10981 tgacgccagc ccggctcggt cgacggtcac gctcgcgggt gctcgcgggg gtggtcgacg
    11041 acctcaccga cgtcgtcgac gcgcaggtgc gggtgaccgt gccggtgctg tcgagcatcg
    11101 tcgccgggct ggtggccacc ctgctcgtgg cgtggttctc ccccgccgtc ggactggtgc
    11161 tggccgggtt gctcgtggcc gtcgccgcca cgtgctggct cgcctggctg ctcgagtccc
    11221 gttcccgtga cgagctgctc gaggcccggg ccgaggtgct gcgggtcagc gacctcgtcg
    11281 cccggcaggc cggtgagctc caggcgatcg gggcgcagga cacggcatcc ggctggctgc
    11341 gcgacgccca cgacgcgctg cggggtgcca cgcggcagca gagccgcggc cgcgccctcg
    11401 tcgcggcgac cctgctcgtc gcgacggccg ccgcgacgat cgccgccgcc ttcctcgccg
    11461 accctgcggc gaccggcgca cccgtcgcgg cgctgctcgt cgtcgtgccg gtggccgtcg
    11521 gggacgcgtt ggcgcccctg gtggacagca tgcgggcgct ggcccgggcc cagggcagcg
    11581 ccgcccgcct cgaggccctg ctcgaccagg agcccgcggt cagcgacccg gtctcggccc
    11641 ctgccacccc cgctgacgtc cggcaggttg gtgcctccag cagccaaggt gccggacgca
    11701 acgcggtgac cgggcgacac gtctccctcg agcaggtcgc agcctcgtgg acgggcaccg
    11761 gagaccagct cgcccccacc gacctcactc tcgagccggg tcagcgcgtc gcggtcgtcg
    11821 ggcccaacgg cagtggcaag agcaccctgc tcgccgtgct cgcccgccac ctcgacccca
    11881 gccgggggcg gcacacggtc gacggcgtcg acgtgcgtga cctgcccctg gacgaggtcc
    11941 gccggcacgt cgccgtcgtc gatgacgaac cccacgtctt cgcctcctcc gtggcggcga
    12001 acgtgcgcct cgcggcaccc gaggcgagcg acgacggcat ccgcaccgct ctcggccgtg
    12061 ccggcctggg cgcctgggtc gacgccctac ccgagggcct ggagacccgt ctcggcaccg
    12121 gcggccgggg ggtgtccggc ggtgagcgca cccggctcgg ggtcgcccgg gcgctcctcg
    12181 ccgaccgccc gctcgtgctg ctcgacgagc ccaccgccca cctcgaccac gcgaccgcca
    12241 ccgcggtgct cgacgacgtc ctcgacgcca ccgatgaccg cgccgtggtg ctcgtcagcc
    12301 accgcccgga ggctgccgaa cgttttcacc gggtgctcga cctcggcgta gcctcgaagg
    12361 ctgccacaac cctgaaggag tagtcatgcc tcactcgacc tcggcacccc gcctcggtga
    12421 cctcgagcgt gtcgtcatgg agcacctctg ggaggcggcc agcagtgggg gcgcggactt
    12481 cgagggcgcc accgtgcgcc aggtccacga ccgcttcgag ggtgagcgcg agatcgccta
    12541 cacgaccgtg atgaccgtgc tcgaccgact ctccaagaag tcgctcgtca cccgagagcg
    12601 tgacggccgc gcctggcgct acctgcccgc cgacacgcgt gaggccctga ccgcgcagac
    12661 gatgcgccgc acgatgaccg acatggatct caccgaccgg cgagctgccc tgctgcactt
    12721 cctcgacggc gcgacgaccg acgagatcgc cgacctcaag gctgctctcg ccgagatcga
    12781 agggcgagcg gcggatgccg cgtcgccgcc ggccgctcga cgtcgcgggc tgcgtcggcc
    12841 ctagtttccg gggctcgggc ccctcgaccg gcggcgttcc ccccactccc cgcacttgtt
    12901 gccctgcagc caccggaccc gggtggctgc agtgcagtaa gtcgggtgct gtcaccattg
    12961 tcggcgtgat cccgctcgcc ctcgcgaccc tggccttgct gctggcctgg gtcgcgcctg
    13021 gcctcatggc ccgccagaag cagttccggc gcgcgccgcg agcatcgctg gtggcgtggc
    13081 aggccgtgac gctcggcgga gtggtggccg cggtggcggt ccccctgtct gccctgccgc
    13141 tggtgatgga ccgtggcgag cctctggccc gggcctgggt cctcgtcccc gcggtgctgg
    13201 ccagcacggc gatcgtcgcc cgactgctct acgcggggca cgacgtcggg accgagctgc
    13261 gccgggtgcg gcaggaccac cggcaactcg tcgacatcat cgccgggcac gacggtgagc
    13321 acgcgcagtt gcggatcctc gagcacccca ccccgacggc ctactgcatc ccggggcggc
    13381 actcgcgggt ggtgttgtcg cagggggtgc tcgactcgtt gccccccgcc gagttgtcgg
    13441 ccgtgctcgc tcacgagcag gcccacctgc gcggtcgcca cgacctgctg ctggagttct
    13501 tcacggtcgt gcaccacgcg gtcccgtccc ggctgcggtc ggcggcggcg ctggccgagg
    13561 tccggctgct cgtcgaggcc ctggccgacc gggccgccgt gcggcgcagc ggtgaggtgg
    13621 cgacggcccg ggccctgatg gcactggccg gaagtcgcac cccggcgggc gagaccgggg
    13681 cgaccctggg tgtcggcacc acggcgccgg tgcggctgcg gctgctggtc gacggtcccc
    13741 cgcacccggc gctgcccgcg gtggcgtacc tgtatgccgc agggtcggcg accctgccgg
    13801 tggccctgct cgtggcggcc tggctctaac gcgcgtcagg cccagctggg tgggggcggc
    13861 ggtggaggcg ggctcccggc atccgacggc ggtggcggtg gcggtggcgg gggcgcagac
    13921 ccggcggccg ctgccgcggc accgggcgga ggcggggggc ctgcctgagc gcccggaggg
    13981 ggcggaggcg cggcagacag ggtcagcgag ccgagctggc cggacgactc gaggccgcgc
    14041 agcgcggcca gcacccggtc acggtcctgc gccgacgacg gccgacccgt cgcctcgagg
    14101 taggtcagca ccccgagatc gcgcagggcc cggtcgccag cagccgacgc ggcgcctccc
    14161 ccgccaaaac caccggtgcc gggcacaccg ccgggggtgc tgatgccggc cttcctcatc
    14221 tcggcatcag ccttcgccgc caggtcgttg gccgcggcct tggctcgctc gaagaaactc
    14281 atggggaacc tccctgatgt ggacctgaat cctgctctga acactaggac cgcacccggg
    14341 ttcgcgcata gcgtgggggg tgatctcgag cgaggggccg ggacacgaca cggaaggtgg
    14401 cagcggcgat gacgattcac gggtccttgt tccacgagtt caaggagaag gagacccagg
    14461 accccttcgc gctgcagaac aagaagctgc tgcgcatcga catgcgctac ggcgaggtct
    14521 gggcccgcac cggctcgatg gtcgcctacc agggggacgt gcgcttcgtg aacaagggct
    14581 cgggcgggtt cggcaagatg ctcaaggccg ctgcgaccgg cgagggcgtg aagatgatgc
    14641 agtgctcggg cagcggcgag ctgttcgtcg ccgacgaggc ctccgagatc cagatcatgt
    14701 acctcgagaa cgacgccctg tcggtcaacg gggccaacgt gctggccttc tccaactcca
    14761 tccagtggga catccaccgc atccaggccc gcggcgccac gatgaccggc gggctgtaca
    14821 acgtgtccct gcgcggcacc gggtacgtcg cggtgacgac caagggtgac ccgatcgccc
    14881 tcgacgtcgc atcgtcgccg acgttcgccg acgcgcaggc cgtcgtactg tggacgtccg
    14941 gggtgtcgat ggacatccgg gtcgacaccg gggggctcgg gtcgatgatc cgcggcggca
    15001 ccggcgagct gctgcagatg gcgttcggcg ggcaggggta cgtgctggtg cagcccagcg
    15061 agtcggtgcc tcagggcggc caccagagcg ggggccagac acccggcggg atgctcggcc
    15121 agttcatgca gtgaccgcct gaccagcacc gcagcgtcct gcagggtggt cgttctggca
    15181 cccaacctgc cggacgcaag gctgcatgcg gaacaatcgg cgccatgacc gcacaggtga
    15241 ccgggaccga catcctcgac gtggcacgtg agcaggtgct cgagcggggc gtgccgctga
    15301 cccaggggca gatcgagcag gtgctgcgca ccggcgacga ccagttgcag gccctgctgg
    15361 cgctcgccca cgaggtgcgc ctgaagtacc agggaccatc ggtcgaggtc gagggcatcg
    15421 tgtcgctgaa gaccggcggc tgcccggagg actgccactt ctgttcccag agtgggcagt
    15481 tcacgagccc ggtgcgttcg gtctggctga acatccccga gctcgtgcgt gccgccaagg
    15541 ccaccgcagc cacgggcgcc agcgagttct gcatcgtcgc cgccgtgcgc gggcccgacg
    15601 acaagctcat ggagcagatg cgcgagggcg tggccgccat ccgcgccgcc gtcgacatca
    15661 acgtcgccgc gtcgctcggg atgctgtcgc gcgagcaggt cgccgacctc gtcgacatgg
    15721 gcgtgcaccg ctacaaccac aacctcgagg ccggccgttc gtacttcccc caggtggtga
    15781 cgacgcactc gttcgaggag cgctgggaca cctgcctcat ggtcaaggag tccggcatgg
    15841 agctgtgctg cggcgggctc gtcggcatgg gcgagaccct cgagcagcgg gccgagctcg
    15901 ccgcacagct cagcgagctc gagccgcacg aggtaccgct gaacttcctc aacccgcggc
    15961 cgggcacgcc cttcggcgac ctcgagccga tggagaccgg cgacgccctg cgcaccatcg
    16021 ccgcgttccg gctcgccctg ccgcgcacga tcctgcgtta cgccggcggc cgcgagctga
    16081 ccctgggcga cctcggcacg cgcgaggggc tgctcggcgg catcaacgcc gtcatcgtcg
    16141 gcaactacct cacgacgctc ggccgcgacc cgcgcgagga cctcgcgctg ctcgacgagc
    16201 tcgagatgcc gatcaaggcg ctcaacgaca cgttctgatc gtcaggtcgg gcgtggggtg
    16261 gtcttcgtga cgaacgggtc ggatgccgtc gggccggtgt cgcgcgccgc tcacccgggt
    16321 gcgtggtgcg gtcagtgcgg tctcgccatg gaggcgcggt cgagcgccga ctctggtgcc
    16381 gcgccgggtg gggacactct ggcgcccgct gacgcccacg cctcgtgtgc ccgccgagcc
    16441 gagctcgagc cgccgcggtt ctgcgcgcac tgccggcgcc ggatgaaggt acaggtcacg
    16501 ccgggtgcct ggacggcgac gtgcgtgcag cacggcaccg tcgagcagcc cacctggtcg
    16561 gcctgacctg aggctcggcc ctcacgcagc actgcagcag cacgcagcag gtcggccgcc
    16621 acgcggcggt ccccctggaa cgcagaagtt cggatcgcac gcagcacttc acctggaacg
    16681 cagcacttca actcgcacgc agcaattacc gcgttcgatc cgagcttgtg cgtgcgatcc
    16741 gaactggcgc gtcgtgacct gctgcggccg agcggaatac gcgtcgatgg ttgttggttc
    16801 aatcacctat gagccttcac gtcctcgttc tcgtcggcag cctgcgcgcc ggctccacca
    16861 acgcccagct cgccgacgcc gccatcgcgc acctgcctgc cggggtcgat ggcacagtct
    16921 tcgcccgact ggcagagctc ccgcactaca gcgaggacct cgaccacgac gactccctcc
    16981 ccgaggtcgc ccgcgacctg cgtgaagccg tcgccgacgc ggatgccgtg ctcctggtct
    17041 ctcccgagta caacggctcg ctgcccggcg tgctcaagaa cgccgtcgac tgggtctccc
    17101 gcccgcgcgg tgctgccgcg atcgcgggca agcccgcggc cgtcatcggc gccagcggct
    17161 cccctcgtgg cgcccagtgg gcccgtgagg acggcatcaa ggtgctcaag gtcgcgggcg
    17221 ccgccccgtt cgaggacacc gtcggtgtgg ggtcggcgtt ccaggccttc cacgacggcc
    17281 gcctcctcga ctccgagctc gacggcgccc tgcgcaccct cgtcacggaa ctcgtcgatg
    17341 ccgcgcagcg cacctctgac tcggaccgcc ccgccgcctg atctcccttg cgtccggcag
    17401 gtggggtgct ccagccacca ccctgccgga cacacgcgag agggcaggca ccccggcggc
    17461 acaatgggcg gcgatgactg ccacccccgc cccgcccccg cgcggccctg acgcgggctc
    17521 cgtcgaggag ctgctcgcgt tcgatcgcga ccacctctgg cacccgtact cctcggccct
    17581 gaccccgcag gatccgtacc tcgtggagtc cgcgcacggc atccggctgc ggttgcgcga
    17641 ccgcgcaggt gagtcgcgcg aggtcatcga cgcgatgtcg tcgtggtggt gcgcgatcca
    17701 cgggtacgcc gtgcccgagc tcgat
//
LOCUS       DAOSOC010000033        17645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3231_length_17645_cov_9.336782, whole genome shotgun sequence.
ACCESSION   DAOSOC010000033 DAOSOC010000000
VERSION     DAOSOC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 17645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17645
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3231_length_17645_cov_9.336782"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4577)
                     /locus_tag="PL014_04430"
     CDS             complement(<1..4577)
                     /locus_tag="PL014_04430"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOT56369.1"
                     /translation="MTDDVLDRFSPATAAWFRGSFSAPTTAQEGAWDAISSGRHALVV
                     APTGSGKTLSAFLWALDRLASEPVPEERLRRCRVLYVSPMKALAVDVERNLRSPLVGI
                     RHAATRLGLPEPDITVAVRSGDTPADERRAFARSPSDVLITTPESLFLLLTSAAREAL
                     AGVETVILDEVHAIAGTKRGAHLALSLERLDALLARPAQRIGLSATVRPVEEVARFLA
                     GGRPVDVVQPPSTKQWDLKVVVPVPDLAEIGQPTSDLSGAASGHEQRTSIWPHVEERI
                     VDLVAAHTSTLVFSNSRRLAERLTSRLNEIWQERTEAAADPGAADAAGPARTPAQVMA
                     QAGASAGAPAVLARAHHGSVSKEQRASIEEDLKSGHLPAVVATSSLELGIDMGAVDLV
                     VQVESPPSVASGLQRVGRAGHQVGAVSRGVLFPKFRGDLVQTAVVVERMLAGEIEALH
                     VPNTPLDVLAQQVVAMCALDEWTVDDVLALARRSAPYATLTRPVLESVLDMLAGRYPS
                     EEFAELRARITWDRLADTLTGRRGAQRLAVTSGGTIPDRGLYAVFLATGEGPGRRVGE
                     LDEEMVYESRVGDLFTLGTSTWRIEDITHDRVLVTPAPGQPGRLPFWKGDSLGRPAEL
                     GRAVGAFVREVEALTPDAARTRVMAAGLDDWAADNLLTYLREQREATGHVPDDRTIVV
                     ERFRDELGDWRVAIHSPFGAQVHAPWALAVSARMRDRFGVDVQAMHGDDGIVLRLPDL
                     EFEDLDGVRERGVGRELLDLVTLDPDDVRALVTEEIGGSALFAARFRECAARALLLPR
                     RQPGRRQPLWQQRQRASQLLEVASQYPSFPIVLEAVRECVQDVFDVPGLVDLMRDITS
                     RRVTVVDVESSTPSPFAKSLLFGYVAQFLYEGDSPLAERRAAALALDPSLLAELLGTS
                     EGLALRDLLDAEQIARTEAELQRLTPDRAARDADDVLDLLRSLGPLPSDGILLRCREG
                     TTHGDVTAWLADLASARQVIPVRVAGEDRWSAIEDASRLRDALGVSLPPGVPQTFLEP
                     VSDPLGDLVARYARTHVPFHAAEVARAYGLGIAVATTALARLVSTGRVVEGELLPTGG
                     GGTEFCDAEVLRMLRRRSLAALRAEVEPVTPVELSRFLLGWQGVGGRLRGREGLLRVV
                     EQLSGAVLPASAIETLVLPSRVADYSPGMLDELMATGEVLWCGHGSLPGDDGWVSLHL
                     VDSAHLTLPGPDPDLVPDEQGLAVLDALAGGGAHFFRTLSDQVGSTDDEALSTTVWGL
                     VWAGRVTGDTFTPVRALLAGGRTAHKRTAAGPRRTRYAGRRGPLGGLGSVPARAPLPS
                     RSGPARVAGRWSLLPPVDLDPTLRAYANAELLLDRHGILTRGATVAEDVPGGFAALYR
                     VLARAEEAGRVRRGYFVEGLGASQFGTTGAVDRLRSGTGGVGATRGDRASAPPAAVVL
                     AASDPANPFGAALPWPERALDPADASGKGTDKRRRHQPGRKAGAVVVLVDGELVLYVE
                     RGGKTLLTSSEDPDLLRAAAGALADAVRRGSL"
     gene            4692..5186
                     /locus_tag="PL014_04435"
     CDS             4692..5186
                     /locus_tag="PL014_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT56370.1"
                     /translation="MQPRWLDDEERAAWLRLVGVVELLPGVLDTQLRQDSGLSHFEYF
                     SLAMLSEAPDHTLRMGALAQRTNATLPRLSHVISRLESRGLVERSPSVKDRRATNARL
                     TAAGWDTVVAAAPGHVDAVRQFVLDPLTREQLRELTVIGDALLARLDPEGRMFGHVIE
                     RPAD"
     gene            complement(5232..5597)
                     /locus_tag="PL014_04440"
                     /pseudo
     CDS             complement(5232..5597)
                     /locus_tag="PL014_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     assembly_gap    5742..5841
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5858..6730
                     /locus_tag="PL014_04445"
     CDS             5858..6730
                     /locus_tag="PL014_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOT56371.1"
                     /translation="MDISRLRLLLELSRLGSMQDVAVELRTSTSTVSQGIAALARDVG
                     TPLIEPDGRRVRLTPAGHRLAEHAVTIIAAVDAARGDLDPSTEPVGAVRVAGFATAIR
                     RSLVPVIDDLATRHPGLQVRVHEYEPAEALDLLARDDVDLALVYDYTLAPASWRSEHD
                     VRPLWEDEWGLGVRVTERRTALADFSGDDWIVNSRHTADEEVLRTLASMAGFTPNVVH
                     RIDALELVDDLVVAGLGVALLPLARHTHPGVRVLPLDPSPLLRGHAVTRLGRDSWPPL
                     RAVLDRLRPAAAAG"
     gene            complement(6705..>7740)
                     /locus_tag="PL014_04450"
     CDS             complement(6705..>7740)
                     /locus_tag="PL014_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOT56372.1"
                     /translation="HWRMPAEWVPHERTWMAWPSSGYTLGESEAAADEARATWADVAL
                     AVSRFEPVTLVVDPDAVVVARRWIGEGRHGHYRVDLLEAPLDDAWMRDIGPTFVVSPT
                     GELGAVDWVFNGWGAQEWATWGNDARIGEFVTGRAGATRVASDLVNEGGGIHVDGLGT
                     VLLTETVQLDPGRNPGLTRADVEAEMARTIGATTCIWLPRGLTRDYDEFGTRGHVDIV
                     ATFASPGVVLLHWQGDPAHPDHEVSVELEGLLSSARDARGERLEVVRVPAPRTLEDED
                     GIVDWSYINHLVVNDGVVACTYDDPQDAASLAVLEKAYAGRQVVGVDARPLFARGGGI
                     HCITQQQPRV"
     assembly_gap    7741..7840
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7841..8541)
                     /locus_tag="PL014_04455"
     CDS             complement(<7841..8541)
                     /locus_tag="PL014_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOT56373.1"
                     /translation="MRLITAASVPPSLARVTEPTAPPLRVGLVQHAWDPDADALRATL
                     TDGIRTAADAGAQIVFLPELTLSRYPADTLPTGRPDTTAESLTDGPTITFAREAAAAT
                     GVLVHASLFERAPAPDGTDDGLGFNTAILVAPDGELLAHTRKTHIPVTAGYYEDKYFR
                     QGPAEGAYPVHEIRATQPPLRLGMPTCWDEWFPEVARAYGLGDAQVLVYPTAIGSEPD
                     HPDFDTQPLWQHTIVG"
     gene            8588..8809
                     /locus_tag="PL014_04460"
     CDS             8588..8809
                     /locus_tag="PL014_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004006267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HOT56374.1"
                     /translation="MRISRFWALAEDEFGAAYAHSLAGSTHLAVLGGRTALEALDAGE
                     SPREVWLALCDAMDVPEERRHGLDKPPKR"
     gene            8831..9796
                     /locus_tag="PL014_04465"
     CDS             8831..9796
                     /locus_tag="PL014_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56375.1"
                     /translation="MRPHRCTTVAAVSDDDVLLRARGLTKTFGDFTAVDGIDVEVRRG
                     EAFGFLGPNGAGKSSTMRMIGCVSPASGGELRILGLDPASDGPAIRARLGVCPQKDIL
                     DEDLSVEDNLHVYGRFFGLSRSEVRERTTALLDFAQLTERRHDRVEPLSGGMKRRLTI
                     ARSLINNPEVLLLDEPTTGLDPQARHVLWDRLFRLKRQGVTLIITTHYMDEAEQLCDR
                     LVVMDKGRIVAQGSPRGLIEEHSTREVLELRFDPDEQAGHLDALADVGERVEVLPDRV
                     LVYTADGDAALATVHARGIRPVSSLVRRSTLEDVFLHLTGRTLVD"
     gene            9796..10653
                     /locus_tag="PL014_04470"
     CDS             9796..10653
                     /locus_tag="PL014_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56376.1"
                     /translation="MSSPTLAEDAVAHPGGSGRLTPGVRPPLSRGERVSSAIAYHHRV
                     YLRTWRGTLIGRFASPLFFLASMGLGLGGLVDDRTGGVDGVPYLRYVAAGILAMQAMW
                     LAFGESTYPVMTYIRWNQMYAGMLATPLSVVEVLLGHLLYVGAHLAMTTAIFLVVAAF
                     FGAFTSAWVVLAVPICVLVGLAFAVPMFAFSAHVDNDNAFAILFRFLVTPVMLFSGVF
                     FPIEQLPSVLQPVAWVLPLGHGVELVRAVSGGLNLGVGDLGHLAVLLAYVGIGWLVAR
                     RAFTKRLAS"
     gene            10650..11468
                     /locus_tag="PL014_04475"
     CDS             10650..11468
                     /locus_tag="PL014_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56377.1"
                     /translation="MSVLDGALQVSRALPLPGALARSWFVVGRNITAFRRAWLLLVSG
                     VAEPIFYLFSIGIGLGALVRTVTTDAGSEVPYAVFVAPALLAVAAMNGAIADSTYNVF
                     FKLKHARLYDAILATPAGPRDIAVGEITWSLLRGSLYSALFLVVAVVAGVVQSWWAVL
                     ALPGAVLIGLAFGAVGMFATTYMKTWQHFEYINLVIQPMFLFSATFFPLSTYPGAVQW
                     LVQITPLYQGVALERALMLGDVGWSLLGHAAYLVAMGLVGVVGTSRRIERLLLT"
     gene            11516..12475
                     /locus_tag="PL014_04480"
     CDS             11516..12475
                     /locus_tag="PL014_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56378.1"
                     /translation="MNDVNELLILNRRDLEGLALTWPEIVDVIEDAFRQKAQGLVTNP
                     PKPKVTSREDAFIHAMPAYLGGSDRIGLKWVSGYEQNHAKGLPYIYGSVIMNDPETGR
                     PIALLDGGWITEMRTPAVSGVTMRHVPLSPKRLAIVGCGVQGRRHLEVALSEHPDIEE
                     VVVFDRSQDAAEVLTSMAGDRVTRIASSPTDAVDGATIAITTITVPLDPKLDCRNTDE
                     NALLLPVDYDDAMATVAFHEAVTYSVDDLGQYGSVAGRKYFFDFPEPVTHLAQVVAGE
                     FEVPEKGRRMFLNMGIAMNDIALSSLVLDRALERGVGTTVEFP"
     gene            complement(12472..13251)
                     /locus_tag="PL014_04485"
     CDS             complement(12472..13251)
                     /locus_tag="PL014_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOT56379.1"
                     /translation="MSETMTEPAAQIPAGGAPLPEAPAPGADAGLRAYEVVLRWVDDR
                     ILAGTLRVGDFLPPERELARMLDVSRSAVREAVRTLQARGVVRSSVGAGAAGGTRITA
                     VPSDALSRLLRQHVALANFPLDDVIEVRIALERLSVRLAATHATADDLAGMRALLDAM
                     ADPDIDRVQFNDRDTEFHVALASAVGNSLARDLTAAIRESMRRPILDRFHALESWAVV
                     APLLCRDHEGIYAAVARGDGTAAAALAEEHIRSAWHLLNPV"
     gene            13423..15165
                     /locus_tag="PL014_04490"
     CDS             13423..15165
                     /locus_tag="PL014_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOT56380.1"
                     /translation="MTATSLAPAAFQPDISPVADSLAISALVALLPLITVFLTLGVLR
                     WKAHWAGLTSVAMAIVVASVFFGMPLDLAVLAATQGAAFGLFPIMWIVFTAILLYQVT
                     VRSGRFEDLRATFHLISDDPRIQAIIIAFCFGGLLEALAGFGAPVAITGVMLMALGFS
                     PLRAAAVVLLANTAPVAFGAVGTPIITAGNLTGIPYQEIGAYVGHQTPILAAFVPLLL
                     VLMADGRRGVRQTWPIAVVVGLTFAIFQWVSATWISVELTDIIASLAGLAAAVLFLKV
                     WQPTGREQALETLHSEREREFAMAGGPETSSGSAGAGPVTDYVVSGAELEERARGLTR
                     GRITMALFPYLLVIVVFAIAKLIAPVKEWLTTTDIKIPWPGLSGEVLTIAGTPSTATT
                     YTLSWLSSPGSMLLICSLIVAFVYGIGLAGWGAEAWATAHKMRYAFLTVASMLALAYV
                     MNLSGQTITIGTWIAATGALFAYLSPTLGWIGTAVTGSDTSANALFATLQQSAAETAG
                     IDPTLLVAANTSGGVVGKMISPQNLTIAATAVGLVGRESDILRKVLPWSIGLIIAMCL
                     LVGLQSTVLAWMLP"
     gene            15150..15932
                     /locus_tag="PL014_04495"
     CDS             15150..15932
                     /locus_tag="PL014_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT56381.1"
                     /translation="MDAPVIAPARPLADLKVALFATCFNDTMWPETPKATVRLLERLG
                     CRVEFPMEQTCCGQMLTNTGYGSKAAPLVRRFVDVFEGYDAVVAPSGSCVGSVREQHR
                     GIVELVGDDRLASRVEHLAPRVYELSEFLVDVLGVTDVGAYFPHRVTYHPTCHSLRML
                     RVGDRPLQLLRAVRGIDLVELPGAQECCGFGGTFAMKNADVSIAMGSDKARHVVETGA
                     EVLVAGDNSCLAHIGGILGRQRSGVRRMHLAEILASTEEDQA"
     gene            15929..17398
                     /locus_tag="PL014_04500"
     CDS             15929..17398
                     /locus_tag="PL014_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HOT56382.1"
                     /translation="MSTTTPGPLQQTSPTFVGMPTFPKAARAALGNTQQRKNLRHATA
                     TIRGKRARVVAEVPEWEALRVAGAEAKDEALTHLGDYLEQLEASLVANGATVHWARDA
                     QEANRIVLSIVRSKEVDEVVKVKSMVTQEIELNEALEAAGIHAWETDLAELIVQLGHD
                     RPSHILVPAIHRNRSEIREIFLREMGTVGRPAPDDLTDDPGRLAEAARLHLREKFLRA
                     KVGVSGANFAVADTGTLVVVESEGNGRMCLTLPETLISVVGIEKVIPSWEDLAPMLQL
                     LPRSSTGERMNPYTTMWTGVHEGDGPQEVHVVLLDNGRSHVLADRVGRQALRCIRCSA
                     CLNVCPVYERTGGHAYGSVYPGPIGAVLNPQLRGTASTIDKSLPYASSLCGACFDACP
                     VRINIPELLVELRGQVVEQEGRTAESAAMRAAAWVLSDPKRLAGAQKAAAAAGRVVGT
                     RTVRSVPGLGAWTNARDLPTPPPESFRQWWARERGEDDR"
     gene            17395..>17645
                     /locus_tag="PL014_04505"
     CDS             17395..>17645
                     /locus_tag="PL014_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HOT56383.1"
                     /translation="MSARDEILARVRGATSDVVTTPDDRGPVPVVEETSPGGPETLEL
                     FAENVADYRAQVVRVGPSGVAGAVADALREHGCSTVVVP"
BASE COUNT         2416 a         6250 c         6282 g         2497 t
ORIGIN      
        1 aggctgccgc ggcgcacggc atccgcgagg gcacccgccg cggcccgcag caggtcaggg
       61 tcctcggagg aggtcagcag ggtcttgcca cccctttcga cgtagagcac gagctcaccg
      121 tcgacgagga cgaccacggc cccggccttg cgtccgggct ggtgacggcg ccgcttgtcg
      181 gtgcccttcc cgctggcatc ggccgggtcg agcgcccgct ctggccacgg cagcgccgca
      241 ccaaaggggt tcgccgggtc actcgccgcg aggacgaccg ccgcgggtgg ggcgctggca
      301 cggtcgccac gggtcgcacc caccccaccg gttccgctac gcagccggtc caccgcgccg
      361 gtcgtgccga actgggaggc acccagccct tcgacgaagt acccccgtcg cacccgaccg
      421 gcctcctccg ccctggccag cacgcggtag agcgcggcga aaccgccggg gacgtcctcg
      481 gccacggtcg cgccccgtgt gaggatgccg tgccggtcga gcagcagctc ggcgttggcg
      541 taggcccgca gcgtcgggtc gaggtcgacc gggggcagca gggaccaccg gccggcgacc
      601 cgtgctggcc cggatcggct gggcaacggc gcccgggcgg gcacggagcc gaggccgccg
      661 agcgggccgc ggcggccggc atacctcgtg cgccgcggac ccgcggccgt gcgcttgtgg
      721 gccgtgcgcc ccccggcgag cagggccctc accggagtga aggtgtcgcc ggtgacgcgc
      781 ccggcccaca cgaggcccca cacggtcgtc gacagcgcct cgtcatcggt ggaacccacc
      841 tggtcggaga gcgtgcggaa gaagtgggcg ccgccgccgg cgagggcgtc gagcaccgcg
      901 agcccctgct cgtccgggac gaggtcgggg tccggcccgg gcagggtgag gtgggcggag
      961 tcgaccaggt gcagcgagac ccagccgtcg tcgccgggca acgacccgtg gccgcaccac
     1021 agcacctcac ccgtggccat gagctcgtcg agcatccccg gggagtagtc ggcgacgcgg
     1081 gacgggagca cgagcgtctc gatcgcgctg gcgggcagca ccgccccgct cagctgctcg
     1141 accacgcgca gcaggccctc gcgaccacgc aggcgcccgc cgaccccctg ccacccgagg
     1201 aggaagcggg agagctcgac cggggtgacc ggctcgacct ccgcacgcag cgcggccagt
     1261 gaccggcggc gcagcatgcg cagcacctcg gcgtcgcaga actccgtgcc gcctccgccc
     1321 gtgggcagca gctcgccctc gacgacccga ccggtggaga ccagccgggc cagcgccgtg
     1381 gtggcgacgg cgatgccgag cccgtaggcg cgggccacct cggcggcgtg gaacggcacg
     1441 tgggtgcgtg cgtagcgggc caccaggtca cccagcgggt cactcaccgg ctcgaggaag
     1501 gtctggggaa ccccgggtgg cagggacacc ccaagggcgt cgcgcaggcg tgaggcgtcc
     1561 tcgatcgctg accagcggtc ctccccggcg acgcgcacgg ggatcacctg ccgggcgctc
     1621 gcgaggtcag cgagccaggc ggtcacgtca ccgtgtgtcg tgccctcccg gcagcgcagg
     1681 aggatgccgt cgctcggcag cgggccgagg gagcgcagca ggtcgagcac gtcgtcggcg
     1741 tcgcgcgcgg cgcggtcggg ggtgagacgc tgcagctcgg cctcggtgcg ggcgatctgc
     1801 tcggcgtcga gcaggtcacg cagggccagg ccctcgctcg tgccgagcag ctcggcgagc
     1861 aggctcgggt cgagggccag ggcagcggcg cgccgctcgg ccagggggga gtcgccctcg
     1921 tagaggaact gggcgacgta cccgaagagc agggacttcg cgaagggcga cggggtcgag
     1981 ctctcgacgt cgaccacggt gacccgtcgc gaggtgatgt cccgcatgag gtcgaccaac
     2041 cccggcacgt cgaagacgtc ctggacgcac tcgcgcacgg cctcgagcac gatggggaag
     2101 gacgggtact ggctggccac ctcgagcagt tgcgaggctc gctgccgttg ctgccagagc
     2161 ggttgccggc gacccggctg gcgacggggc agcagcagcg cacgagcggc gcactcgcgg
     2221 aagcgggcgg cgaacagggc cgatccgccg atctcctcgg tgaccagggc acgcacgtca
     2281 tcggggtcga gggtgacgag gtcgagcagc tcgcggccga cgccgcgctc gcgcaccccg
     2341 tcgaggtcct cgaactcgag gtcgggcagg cgcagcacga tgccgtcgtc gccgtgcatg
     2401 gcctgcacgt cgacgccgaa gcggtcacgc atccgggcgc tgacggccag ggcccagggg
     2461 gcgtgcacct gggcaccgaa gggggagtgg atcgccaccc gccagtcgcc gagctcgtcg
     2521 cggaagcgct cgacgacgat ggtgcggtcg tccgggacgt ggccggtggc ctctcgctgc
     2581 tcgcgcaggt aggtgagcag gttgtcggcc gcccagtcgt cgagcccggc cgccatgacg
     2641 cgggtgcgcg cggcatccgg ggtcaacgcc tcgacctcgc gcacgaaggc gccgaccgca
     2701 cggccgagct cggcggggcg gccgagcgag tcgcccttcc agaacggcag ccggccgggc
     2761 tgtcccggtg cgggggtgac gagcaccctg tcgtgggtga tgtcctcgat gcgccacgtg
     2821 ctcgtcccga gggtgaacag gtcaccgacc cgcgactcgt agaccatctc ctcgtcgagc
     2881 tcgccgaccc ggcggcccgg gccctcaccg gtggcgagga agacggcgta gaggccacga
     2941 tcggggatcg tcccgccgga cgtgaccgcg aggcgctggg cgccgcgccg gccggtgagc
     3001 gtgtcggcga ggcgatccca cgtgatccgg gcgcgcagct cggcgaactc ctcgctcggg
     3061 tagcgaccgg ccagcatgtc cagcaccgac tccagaacgg gccgggtgag ggtggcgtac
     3121 ggggcgctgc gacgcgcgag cgccaggacg tcgtcgacgg tccactcgtc gagggcgcac
     3181 atcgccacca cctgctgggc gaggacgtcg agaggggtgt tgggcacgtg cagcgcctcg
     3241 atctcaccgg cgagcatgcg ctcgacgacg acggcggtct ggacgaggtc gccccggaac
     3301 ttggggaaga gcaccccacg gctcacggcg ccgacctggt gcccagcacg gccgacgcgc
     3361 tggagccccg aggcgaccga cggcggcgac tcgacctgga cgacgaggtc gactgcgccc
     3421 atgtcgatgc cgagttcgag gctgctcgtc gcgaccaccg cgggaaggtg gccggacttg
     3481 aggtcctcct cgatgctcgc gcgctgctcc ttgctcaccg agccgtggtg ggcccgggcc
     3541 agcaccgccg gcgcgcctgc ggaggcgccg gcctgggcca tcacctgtgc cggtgtgcga
     3601 gcggggccag cagcatctgc cgcacccggg tcggcggccg cctcggtgcg ttcctgccag
     3661 atctcgttga gccgcgacgt gaggcgctca gcgagtcggc ggctgttgga gaacaccagg
     3721 gtcgaggtgt gggcggccac gaggtcgacg atgcgttcct cgacgtgggg ccagatcgac
     3781 gtgcgctgct cgtgccccga cgcggcaccg gacaggtcgc tggtgggctg gccgatctcg
     3841 gcgaggtcgg ggaccggcac gacgaccttg aggtcccact gcttggtcga gggcggctgg
     3901 acgacgtcga ccgggcgacc gccggcgagg aagcgggcga cctcctcgac ggggcgaacc
     3961 gtggcagaca acccgatgcg ctgtgcgggg cgggcgagca gggcgtcgag ccgttcgaga
     4021 ctgagggcga ggtgagcccc acgcttggtg cccgcgatgg cgtggacctc gtcgaggatg
     4081 accgtctcca cccccgcgag ggcctctcgg gccgcggagg tgaggaggag gaagagtgac
     4141 tcgggggtgg tgatgagcac gtcgctgggg gagcgcgcga aggcccggcg ctcgtcggcg
     4201 ggagtgtcgc cggagcggac cgccacggtg atgtcgggtt cgggcagccc caggcgggtc
     4261 gccgcgtggc ggatgccgac cagcgggctg cgcaggtttc gctcgacgtc gacggccaac
     4321 gccttcatgg gcgagacgta caggacccgg caccggcgca gcctctcctc gggcaccggt
     4381 tcgctcgcga ggcggtcgag ggcccacagg aacgcggaca gggtcttgcc ggagccggtg
     4441 ggtgcgacga cgagggcgtg gcggccgctg ctgatggcgt cccaggcccc ctcctgggcg
     4501 gtggtcggcg cgctgaagct tccccggaac cacgcagccg tggcggggga gaagcggtcg
     4561 aggacgtcat cggtcatgtc gggcacaacc ctctcacccg gctccgacag cggggccctc
     4621 gagagcaacc ggcacgaggg cgcatgccct tacgacgctt gcggcttcaa caatcctcta
     4681 tgctctggcg catgcaaccg cggtggctcg atgacgagga acgcgcggcg tggctgcgcc
     4741 tcgtcggtgt cgtcgagttg ctgcccggtg tcctggacac ccagttgcgc caggactccg
     4801 gcctgagcca cttcgagtac ttcagcctgg cgatgttgtc agaggcccct gatcacaccc
     4861 tgcgcatggg ggccctcgcc cagcgcacga acgccaccct gccgcgcctg tcccacgtca
     4921 tctcacgcct ggagagcaga gggctcgtcg agcgctcacc gagcgtcaag gaccggcgag
     4981 ccaccaacgc gcggctgacc gccgccggat gggacaccgt cgtcgccgcc gcgccgggcc
     5041 acgtcgacgc cgtgcgccag ttcgtcctcg accccctcac cagggagcag ctgcgcgagc
     5101 tgacggtgat cggggacgcg ctgctcgccc gcctcgaccc cgagggccgc atgttcggcc
     5161 acgtcatcga gcgacccgcc gactgaggcc cgcgcgccac tgcgctcggc accgatgccg
     5221 gcggcgacga ccagggcgat cccggcgacg gccagggcgc tcggcacctg ggccagggcg
     5281 aggaacccga tgaccagggc gaaggccggc tcgagtgcca tgagggtgcc gaacgcggcg
     5341 gtggtgagcc gccgcagggc cagcagctcg agcacgaagg ggatgacggg cagcaggatc
     5401 gccagcccca ggccgatgag gagcagctgc ggggtgaggt ccgcgacgac gcccggaccg
     5461 gccaccacgg tcgcgacgac cgcggccacc ggcatcgaga tcgccaatcc cgcgatgccg
     5521 ctcaccgagt cacctacccg ttgggtgagc aggatgtagg ccgcccagca cacggctgcg
     5581 ccgagggcgc aggccacgcc gaccgggtcg gccgtgccgg cccagggctc ggtgagcagc
     5641 aggacgccgg ctgaggccac cagggcccag gcccgtgcga ccccgcggct gcgggcgatc
     5701 gccacgccga gcgggccgag gaactcgatg gcgctggccg tnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nccgggacaa tcggtgggtg gacatctcac ggctgcggct
     5881 tctcctcgag ctctcgcggc tcggctccat gcaggacgtc gccgtcgagc tgcgcacgag
     5941 cacctcgacg gtctcgcagg gcatcgccgc cctcgcccgt gacgtgggga ccccgctcat
     6001 cgagcccgac ggccgacggg tacggctgac ccccgccggc caccggttgg ccgagcacgc
     6061 ggtgacgatc atcgccgccg tcgacgcggc tcgcggtgac ctcgacccga gcaccgagcc
     6121 ggtcggcgcc gtccgggtcg ccgggttcgc gacggcgatc cgccgctccc tcgtgccggt
     6181 catcgacgac ctcgcgaccc ggcatccggg cctgcaggtg cgggtccacg agtacgagcc
     6241 ggccgaggcc ctggacctgc tcgcccgtga cgacgtcgac ctggcgctgg tctacgacta
     6301 cacgctggct ccggcgtcgt ggcgaagcga acacgacgtc cgcccgctct gggaggacga
     6361 gtggggactc ggggtccggg tcaccgagcg ccgcacggca ctcgccgact tctccggtga
     6421 cgactggatc gtcaactcgc gccacaccgc cgacgaggag gtgctgcgca cgctcgcctc
     6481 gatggcgggc ttcaccccga acgtcgtcca ccggatcgac gccctcgagc tcgtcgacga
     6541 cctcgtcgtc gcaggcctgg gggtggcact tctcccgctc gcacggcaca cccacccggg
     6601 ggtgcgcgtg ctaccgctcg acccgtcgcc gcttctcagg ggacatgccg tgacccggtt
     6661 gggcagggac agctggccgc cgcttcgtgc cgtgctcgac cgcctcagac ccgcggctgc
     6721 tgctgggtga tgcagtggat gccgccgccg cgggcgaaca acggccgggc atccaccccg
     6781 acgacctgcc gaccggcata cgccttctcc agcacggcca gcgacgcggc atcctgcggg
     6841 tcgtcgtagg tgcaggccac gaccccgtcg ttgacgacca ggtggttgat gtaggaccag
     6901 tcgacgatgc cgtcctcgtc ctcgagggtg cgcggtgccg ggacgcgcac gacctcgagc
     6961 cgctcgccgc gggcgtcgcg ggccgaggac agcagaccct cgagctcgac ggacacctcg
     7021 tggtcggggt gggccgggtc gccctgccag tgcagcagca cgacgccggg ggaggcgaag
     7081 gtcgcgacga tgtcgacgtg cccgcgggtg ccgaactcgt cgtagtcgcg ggtcaggccg
     7141 cgaggcagcc agatgcacgt cgtcgcgccg atggtgcggg ccatctcggc ctcgacgtcg
     7201 gcccgggtca ggcccgggtt gcggcccggg tcgagctgca ccgtctcggt gagcagcacc
     7261 gtacccaggc cgtcgacgtg gatgccgccg ccctcgttca cgaggtcgct cgccacccgc
     7321 gtcgcgccgg cgcgcccggt gacgaactcg ccgatgcggg cgtcgttccc ccaggtcgcc
     7381 cactcctggg caccccagcc gttgaacacc cagtcgaccg cgcccagctc acccgtgggc
     7441 gacacgacga aggtcggccc gatgtcgcgc atccacgcgt cgtcgagcgg cgcctcgagc
     7501 aggtcgaccc ggtagtggcc gtgccgcccc tcgccgatcc agcggcgggc gacgacgacg
     7561 gcatccgggt cgacgacgag ggtgacgggc tcgaaccgcg acaccgcgag cgcgacgtcg
     7621 gcccacgtgg cccgcgcctc gtcggcggcg gcctcgctct ctccgagggt gtagcccgat
     7681 gacggccacg ccatccacgt gcgctcgtgc ggcacccact cggccggcat ccgccagtgg
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgacgatgg tgtgctgcca
     7861 cagcggctgg gtgtcgaagt cggggtggtc gggctccgag ccgatcgcgg tcgggtagac
     7921 cagcacctgc gcgtcgccga ggccgtaggc gcgggccacc tcggggaacc actcgtccca
     7981 gcacgtcggc atgcccaggc gcagcggagg ctgcgtggcc ctgatctcgt gcacggggta
     8041 ggcgccctcg gccgggccct gccggaagta cttgtcctcg tagtacccag ccgtcacggg
     8101 gatgtgggtc ttgcgggtgt gggcgagcag ctcgccgtcc ggggccacga ggatcgcggt
     8161 gttgaagccg aggccgtcgt cggtgccgtc gggggcgggc gcccgctcga acagtgaggc
     8221 gtgcacgagc accccggtgg ccgcggccgc ctcgcgcgcg aaggtgatgg tcggcccgtc
     8281 ggtgagtgac tcggcggtgg tgtcggggcg gccggtcggc agcgtgtcgg cggggtagcg
     8341 cgacagggtg agctcgggca ggaagacgat ctgggccccg gcgtcggcgg cggtgcggat
     8401 gccgtcggtc agggtcgcgc gcaacgcgtc ggcgtcgggg tcccaggcgt gctggaccag
     8461 cccgacccga agtgggggag ccgtgggctc ggtgacgcgg gcgagggacg ggggcaccga
     8521 agcggcggtg atcaggcgca tgcgggtcag tatgccgctg acctcgggca cagcctacga
     8581 tcgccggatg cgcatctccc ggttctgggc cctcgccgag gacgagttcg gtgctgccta
     8641 cgcgcactcc ctcgccggct cgacgcacct cgccgtgctc ggcggccgga cggcgctcga
     8701 ggcgttggat gccggtgagt cacctcgcga ggtctggctc gccctgtgcg acgcgatgga
     8761 cgttcccgag gagcggcggc acggcctcga caagccgccg aaacgctgac gcccgcggga
     8821 aatacggtcg atgcggccac accgctgcac taccgtcgct gcggtgagcg atgacgacgt
     8881 cctgctgcgc gcccgtgggc tgaccaagac cttcggcgac ttcaccgcgg tcgacggcat
     8941 cgacgtcgag gtgcgccgcg gcgaggcgtt cggtttcctc gggcccaacg gtgccggcaa
     9001 gtcgagcacg atgcggatga tcggctgcgt ctcgccggcc agtggtggtg agctgagaat
     9061 cctcggtctc gacccggcat ccgacgggcc ggcgatccgg gcacgcctgg gggtgtgccc
     9121 gcagaaggac atcctcgacg aggacctgag tgtcgaggac aacctgcacg tgtacggccg
     9181 gttcttcggg ctctcccgct ccgaggtgcg tgagcgaacc accgccctgc tggacttcgc
     9241 gcagctcacc gagcgccgcc acgaccgggt cgagccgttg tcgggaggga tgaagcggcg
     9301 cctgacgatc gcccgcagcc tcatcaacaa tccggaggtg ctgctcctgg acgagcccac
     9361 gaccgggctg gacccgcagg cgcggcacgt gttgtgggac cggctcttcc ggctcaagcg
     9421 tcagggcgtg accctcatca tcacgacgca ctacatggac gaggccgagc agctgtgcga
     9481 ccgcctcgtg gtcatggaca aggggcgcat cgtcgcgcag ggcagcccgc gcggcctcat
     9541 cgaggagcac agcacgcgcg aggtgctcga gctgcgcttc gaccccgacg agcaggcggg
     9601 ccacctcgat gccctcgccg acgtggggga gcgcgtcgag gtgctgcccg acagggtgct
     9661 cgtctacacc gccgacgggg acgcggcgct ggcgacggtg cacgcgcgcg gcatccggcc
     9721 ggtgtcctcg ctcgtgcgcc gctccacctt ggaggacgtc ttcctccacc tcaccggccg
     9781 aacgctggtc gactgatgag cagcccgacc ctcgccgagg atgccgtggc acaccccggc
     9841 gggtccggtc gcctcacccc gggggttcgt ccaccgctgt cgcgcggtga acgggtgtcc
     9901 tcggcgatcg cgtaccacca ccgggtctac ctgcgcacgt ggcgcggcac cctcatcggt
     9961 cgcttcgcgt cgccgctgtt cttcctggcc tcgatggggc tcgggctcgg tgggctcgtc
    10021 gacgaccgga cggggggcgt cgacggggtg ccgtacctgc gctacgtggc ggccggcatc
    10081 ctggcgatgc aggctatgtg gctcgcgttc ggggagtcga cgtacccggt gatgacgtac
    10141 atccggtgga accagatgta cgcgggcatg ctcgcgaccc cgctgtcggt ggtcgaggtg
    10201 ctgctcgggc acctgctgta cgtcggggcg cacctggcga tgaccaccgc gatcttcctc
    10261 gtcgtcgcgg cgttcttcgg ggccttcacc tcggcgtggg tggtcctcgc cgtgccgatc
    10321 tgcgtcctcg tcgggttggc gttcgcggtg ccgatgttcg ccttctcggc gcacgtcgac
    10381 aacgacaacg ccttcgccat cctgttccgg ttcctcgtca ccccggtcat gctgttctcg
    10441 ggcgtgttct tccccatcga gcagctgccg tcggtgctgc aaccggtggc gtgggtgctg
    10501 ccgctcgggc acggggtcga gctcgtgcgg gcggtctcgg gcgggctgaa cctcggtgtg
    10561 ggcgacctcg ggcacttggc cgtgctcctc gcgtacgtcg ggatcggctg gttggtggcg
    10621 cggcgcgcct tcacgaagcg gctcgcgtca tgagcgtgct cgacggcgcg ctgcaggtct
    10681 cgcgagccct gccgcttccg ggggctctcg cgcggtcctg gttcgtcgtg gggcgcaaca
    10741 tcaccgcgtt ccgccgggcc tggctgctgc tcgtcagcgg ggtggccgaa ccgatcttct
    10801 acctgttctc catcggcatc gggctcggtg cgctcgtgcg caccgtgacc acggatgcgg
    10861 gaagcgaggt gccctacgcg gtcttcgtcg ccccggcgct gctcgcggtc gcggcgatga
    10921 acggcgcgat cgccgacagc acgtacaacg tgttcttcaa gctcaagcac gcccgcctct
    10981 acgacgcgat cctcgccacc cccgcaggcc cgcgtgacat cgccgtcggc gagatcacct
    11041 ggtcgctgct gcgcgggtcg ctgtactcgg cgctcttcct ggtcgtggcg gtcgtcgccg
    11101 gggtcgtgca gtcgtggtgg gcggtgctcg cgctgcccgg ggcggtcctc atcggtctgg
    11161 ccttcggggc ggtcgggatg ttcgcgacga cgtacatgaa gacctggcag cacttcgagt
    11221 acatcaacct cgtcatccag ccgatgttcc tgttctcggc gacgttcttc cccctgtcga
    11281 cgtacccggg ggcggtgcaa tggctggtgc agatcacccc gctgtaccag ggggtcgcgc
    11341 tcgaacgcgc cctcatgctc ggtgacgtcg gctggtccct gctcggtcac gccgcgtacc
    11401 tcgtcgccat gggtctcgtg ggggtcgtcg gcacctcacg gcgcatcgag cggctgctgc
    11461 tcacctgagg tcgccctgac gagcgcgaac cgatggtgcg cgatacgttg cggccatgaa
    11521 tgatgtcaac gagcttctga tcctcaaccg ccgcgacctc gaaggtctcg ccctgacgtg
    11581 gccggagatc gtcgacgtca tcgaggacgc cttccggcag aaggcgcagg ggctcgtgac
    11641 gaacccgccc aagcccaagg tcacctcccg ggaggacgcc ttcatccacg ccatgccggc
    11701 ctacctcggg ggcagcgacc gcatcgggct gaagtgggtc tcgggctacg agcagaacca
    11761 cgccaagggc ctgccctaca tctacggctc ggtcatcatg aacgaccccg agaccggccg
    11821 cccgatcgcc ctgctcgacg gcggctggat caccgagatg cgcacacccg ccgtctcggg
    11881 cgtgacgatg cgccacgtgc cgctctcgcc gaagcggttg gcgatcgtcg ggtgcggcgt
    11941 ccagggtcgc aggcacctcg aggtggccct gagtgagcac cccgacatcg aggaggtcgt
    12001 cgtgttcgac cgctcgcagg atgctgccga ggtgctgacc tcgatggccg gtgatcgcgt
    12061 gacgcgcatc gcgtcgtcgc cgacggatgc cgtggacggg gcgaccatcg cgatcacgac
    12121 gatcacggtg ccgctggacc ccaagctcga ctgccgcaac accgacgaga acgcgctgct
    12181 gctgcccgtc gactacgacg acgccatggc gaccgtcgcc ttccacgagg cggtcacgta
    12241 ctcggtcgac gacctcgggc agtacggctc ggtggcgggc cgcaagtact tcttcgactt
    12301 ccccgagccg gtgacccacc tggcgcaggt cgtggcgggc gagttcgagg tgccggagaa
    12361 ggggcggcgg atgttcctga acatgggcat cgccatgaac gacatcgcgt tgtcctcgct
    12421 cgtgctcgac cgagcgctcg agcggggggt cgggaccacc gtcgagttcc cctagacggg
    12481 gttcaggagg tgccacgctg accggatgtg ctcctcggcc agtgctgcgg cggccgtgcc
    12541 gtccccgcgg gcgaccgccg cgtagatccc ctcgtggtcg cggcacagca ggggcgcgac
    12601 gacggcccat gactcgaggg catggaaccg gtcgaggatg ggacgccgca tcgactcgcg
    12661 gatggcagcg gtgaggtcgc gggcgaggct gttcccgacc gccgaagcca gtgccacgtg
    12721 gaactcggtg tcgcggtcgt tgaactgcac cctgtcgatg tccgggtctg ccatcgcgtc
    12781 cagcagggct cgcatcccgg cgaggtcgtc agcagtcgcg tgggtcgcgg cgagccgcac
    12841 actgagacgc tccagggcga tccgcacctc gatgacgtcg tccagcggaa agttcgccag
    12901 agccacgtgc tgacgcaaca gccggctcag cgcgtcgctc ggcacagcgg tgatcctcgt
    12961 gccgccggca gccccggcac ccaccgagga gcgcaccacg ccgcgcgcct ggagagtgcg
    13021 aacggcctct cgcaccgcgg accggctgac gtcgagcatc cgtgccagct cgcgctccgg
    13081 cggcaggaag tcacccaccc gcagcgtgcc tgcgaggatg cggtcgtcga cccagcgcag
    13141 cacgacctcg taggcccgga ggccggcgtc ggcgcccggt gcgggcgcct ccggcagggg
    13201 agccccacct gcaggaatct gggccgcggg ctcggtcatc gtctcgctca cgagtccagc
    13261 atggcacgac gaccgaccgc tagacccaga ggtcagacca ttcgttgcag gagtggtcag
    13321 accatttgac tcccgcctcg ggcgtcacta ccgttccccc aatcccgaca tggtcggacc
    13381 tattgtggga tcgagcccaa tgacgtgcgc ctcggaggac caatgaccgc tacatccctc
    13441 gcccccgctg ccttccaacc ggacatctca ccggtggcgg acagcctggc gatctcggcc
    13501 ctcgtggccc tgctccccct gatcaccgtg ttcctcacgc tcggtgtcct gcggtggaag
    13561 gcccactggg ccggcctgac gtcggtggcc atggccatcg tcgtggcctc cgtgttcttc
    13621 gggatgccgc tcgacctcgc cgtcctcgcg gccacccagg gcgccgcgtt cggtctcttc
    13681 ccgatcatgt ggatcgtctt cacggcgatc ctgctctacc aggtgacggt gcgatccggc
    13741 cggttcgagg acctccgtgc gacgttccac ctcatctccg acgacccccg catccaggcg
    13801 atcatcatcg ccttctgctt cggtggactc ctcgaggcct tggccggctt cggcgccccc
    13861 gtggcgatca ccggcgtcat gctcatggcc ctgggcttct cgcccctgcg cgccgccgcc
    13921 gtcgtgctcc tggccaacac cgcgcccgtc gcgttcggtg cggtcggcac cccgatcatc
    13981 accgcgggca atctcaccgg catcccttac caggagatcg gcgcgtacgt cgggcaccag
    14041 accccgatcc tcgcggcgtt cgtcccgctc ctgctcgtcc tcatggccga cggccgccgc
    14101 ggcgtgcgcc agacctggcc gatcgcggtc gtcgtcggcc tcaccttcgc gatcttccag
    14161 tgggtctccg cgacctggat ctccgtcgag ctgaccgaca tcatcgcctc cctcgcgggt
    14221 cttgccgcag ccgtgctctt cctcaaggtc tggcagccca cgggccgcga gcaggccctg
    14281 gagaccctgc actccgagcg cgagcgcgag ttcgccatgg ccggcggtcc cgagacgtcc
    14341 agcggctcgg ccggggccgg ccccgtcacc gactacgtcg tgtccggtgc cgagctcgag
    14401 gagcgggccc ggggcctgac ccgtggacgg atcacgatgg cgctgttccc ctacctgctc
    14461 gtcatcgtcg tcttcgccat cgccaagctg atcgctccgg tcaaggagtg gttgaccacg
    14521 accgacatca agatcccctg gccggggctg tcgggcgagg tcctcacgat cgccggtacg
    14581 cccagcacgg cgacgaccta cacgctgtcc tggctcagct cgccgggctc gatgctcctc
    14641 atctgctcgc tcatcgtcgc tttcgtctac ggcatcggcc tggccggatg gggagccgag
    14701 gcctgggcca ccgcgcacaa gatgcgctac gccttcctca cggtcgcctc gatgctcgcc
    14761 ctggcctacg tcatgaacct ctcgggccag acgatcacga tcggcacctg gatcgcggcg
    14821 acgggcgccc tgttcgccta cctcagcccg actctcgggt ggatcggtac cgccgtcacc
    14881 gggtcggaca ccagcgccaa cgccctgttc gccacgctcc agcagagcgc cgcggagacc
    14941 gccggcatcg acccgacgct gctcgtcgcc gccaacacct ccggcggagt cgtcggcaag
    15001 atgatcagcc cgcagaacct gaccatcgcg gccacggccg tgggtctggt cggccgcgag
    15061 tccgacatcc ttcgtaaggt cctgccatgg agcatcggcc tgatcatcgc catgtgtctc
    15121 ctcgtcggtc tccagtcgac cgtcctggcg tggatgctcc cgtgatcgcg ccggctcgcc
    15181 cgctggccga cctcaaggtc gccctgttcg cgacgtgctt caacgacacg atgtggcccg
    15241 agacacccaa ggccacggtc cggctcctcg agcggctcgg atgccgggtc gagttcccga
    15301 tggagcagac gtgctgcggg cagatgctca ccaacaccgg ctacggctcg aaggccgcgc
    15361 cgctcgtgcg ccggttcgtc gacgtcttcg agggctacga cgccgtcgtg gcgccgtcgg
    15421 gctcgtgcgt cgggtcggtg cgcgagcagc accgcggcat cgtcgagctg gtcggtgacg
    15481 accggctggc cagccgggtc gagcacctcg cgccgagggt ctacgagctg tcggagttcc
    15541 tcgtcgacgt cctcggcgtg accgacgtgg gcgcctactt cccgcaccgc gtcacctacc
    15601 acccgacgtg ccactcgctg cggatgctcc gggtcggaga ccgaccgctc cagctgctgc
    15661 gggccgtgcg cggcatcgac ctcgtcgagc tgcccggcgc gcaggagtgc tgcggcttcg
    15721 gcggcacgtt cgccatgaag aacgccgacg tctccatcgc gatgggttcg gacaaggcgc
    15781 ggcacgtcgt ggagaccggc gccgaggtgc tcgtcgccgg cgacaactcg tgcctggcgc
    15841 acatcggcgg gatcctcggc aggcaacggt ccggcgtgcg ccggatgcac ctggccgaga
    15901 tcctcgcctc caccgaggag gaccaggcat gagcaccacg actcccgggc cgctgcagca
    15961 gacctcgccg accttcgtcg ggatgccgac gttcccgaag gcggcccgcg ccgcgctggg
    16021 caacacccag cagcgcaaga acctgcgtca cgcgacggcc acgatccgcg gcaagcgggc
    16081 ccgggtcgtg gcggaggtcc ccgagtggga ggcgctgcgg gtggccggag ccgaggccaa
    16141 ggacgaggcg ctcacccacc tcggcgacta cctcgagcag ctcgaggcga gcctggtcgc
    16201 gaacggcgcc acggtgcact gggcgcgcga tgcgcaggag gccaaccgga tcgtcttgtc
    16261 catcgtgcga tccaaggagg tcgacgaggt cgtcaaggtc aagtcgatgg tgacccagga
    16321 gatcgagctc aacgaggcgt tggaggctgc cggcatccac gcgtgggaga ccgacctcgc
    16381 cgagctcatc gtccagctcg gacacgaccg cccgagccac atcctcgtcc cggcgatcca
    16441 ccgcaaccgc agcgagatcc gcgagatctt cctgcgcgag atgggcaccg tgggccgccc
    16501 cgcccccgac gacctcaccg acgaccccgg acggctcgcc gaggccgccc ggctgcacct
    16561 gcgcgagaag ttcctgcgcg cgaaggtcgg tgtctcgggg gccaacttcg cggtcgccga
    16621 cacgggcacg ctcgtcgtcg tcgagtccga gggcaacggc cggatgtgcc tgacgctgcc
    16681 cgagacgctc atctcggtcg tcggcatcga gaaggtcatc ccctcgtggg aggacctcgc
    16741 gccgatgctc cagctgcttc cccgttcgag cacgggggag cggatgaacc cgtacacgac
    16801 gatgtggacc ggggtccacg agggagacgg accccaggag gtgcacgtcg tgctcctgga
    16861 caacggccgc agccacgtgc tcgccgaccg cgtcggccgc caggcgctgc ggtgcatccg
    16921 ctgctccgcg tgcctgaacg tctgtcccgt gtacgagcgc accggcgggc atgcgtacgg
    16981 ctcggtctac ccggggccga tcggcgcggt gctcaacccg cagctgcggg gcaccgccag
    17041 cacgatcgac aagtcgctgc cctatgcctc gagcctgtgt ggagcgtgct tcgacgcgtg
    17101 ccccgtgcgg atcaacatcc ccgagctgct cgtcgagctg cgcgggcagg tcgtggagca
    17161 ggaagggcgc accgccgagt ccgccgcgat gcgggccgcg gcctgggtgc tgtcggaccc
    17221 caagcggctc gccggcgcgc agaaggcggc ggcggccgcg ggccgtgtcg tcggcacgcg
    17281 cacggtccgc tccgtgcccg ggctcggcgc ctggaccaac gccagggacc tgcccacacc
    17341 accacccgag tccttccggc agtggtgggc ccgcgaacgg ggagaggacg accgatgagc
    17401 gcccgcgacg agatcctcgc ccgggtcagg ggcgcgacct ccgacgtcgt cacgacgccc
    17461 gacgaccgag gccccgtgcc cgtggtcgag gagacctctc ccggcgggcc cgagacgctc
    17521 gagctgttcg ccgagaacgt cgccgactac cgcgcgcagg tcgtgcgggt tggcccgtca
    17581 ggcgtcgccg gtgccgtcgc cgatgcgttg cgcgagcacg gctgtagcac cgtcgtcgtg
    17641 ccgag
//
LOCUS       DAOSOC010000034        17633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3234_length_17633_cov_10.474627, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000034 DAOSOC010000000
VERSION     DAOSOC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 17633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17633
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3234_length_17633_cov_10.474627"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PL014_04510"
     CDS             complement(<1..329)
                     /locus_tag="PL014_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOT56384.1"
                     /translation="MSVEARPGADDPYVALQETDEFQNLRKKFRGFVFPMTAFFLTWY
                     FLYVLLSIFAPGFMNKPISGLVTVGLVFGLLQFVTTFAITFIYARWAGRTFDPAAEAL
                     GARMDAMS"
     gene            536..1576
                     /gene="tdh"
                     /locus_tag="PL014_04515"
     CDS             536..1576
                     /gene="tdh"
                     /locus_tag="PL014_04515"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOT56385.1"
                     /translation="MRALRKVTAGPGLELVEIPEPECGDKDVKIRVLRAGLCGTDLHL
                     EQWDEWAAATVRAPMTLGHEFFGEVVEVGADVDHVAVGDRVSGEGHLVCGTCRNCRAG
                     RRHLCINTVGVGVNRDGAFADYVVIPATNAWVQPPDLDPDLGALFDPLGNATHTALQW
                     PVVGEDVVITGAGPIGVMATAVVRHAGARRIVVTDMSAGRLALAAAAGADLVVDVSAG
                     QTLRDAQGQLGMAEGFDIALEMSGSPAAVEDLLANMNHGGRVAMLGLPKDRYAIDWGR
                     VITHMITLRGIYGREMFETWYLMGSMLATSPELRTAVSSVVTGRYAAAEWQAAFDETR
                     SGTGGKVVLDWS"
     gene            1688..2881
                     /locus_tag="PL014_04520"
     CDS             1688..2881
                     /locus_tag="PL014_04520"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOT56386.1"
                     /translation="MYGTVKDELAATLKEIEDAGLYKRERELTTPQAAHVATAGGSSL
                     NFCANNYLGFADHPDVVAASRNALDEWGFGMASVRFICGTQTQHTLLEQRLSQFLGME
                     ATILFGSCFDANGGVFEVLFGAEDAIVSDELNHASIIDGIRLSKAARFRYKNADMGDL
                     RAQLEAARAGGARRVCVVTDGVFSMDGSYAPLDQICDLADEFQAMVLVDDSHAVGFVG
                     EGGRGTPELFGVMDRVDIITGTLGKALGGASGGYVTSHQEVVDLLRQRARPYLFSNSV
                     APAVVAGSLAALDLVSGSSEARETLRANSALFRELMTDAGFELLPGSHPITPVMFPGE
                     DGARQAAEVADHMLAAGVYVVAFSFPVVPRGRARIRVQLSAAHSEADVRECVDAFVAA
                     RAAVG"
     gene            complement(2984..4513)
                     /locus_tag="PL014_04525"
     CDS             complement(2984..4513)
                     /locus_tag="PL014_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOT56387.1"
                     /translation="MNNPLSVAAVALVCLTTLIVGGLGLRMSRRTSDFYVAGRTVSPR
                     MNASAIGGEYLSAASFLGVAGLVYDRGVDMLWLPVGYTLGYLVLLVVVAAPMRRSGAY
                     TLPDFAEARLGSRAIRLVSSTLVIAIGWLYLVPQFQGASLTLTLVTGAPTWVGGFIVM
                     VVIALAVGAGGMRSITFVQAVQYWIKLTALAVPAFVLLALWFRSGSPAPQVDPSWHLP
                     LRPTGPDDHPWYRTASTVLALCLGTMGLPHVLVRYYTNPDGNGARRTTVTVLALLGAF
                     YVIPPVYGALGRIAYPTLPDGMGSDTIVLQLPETMVDGLAGQLLTAMLAGGAFAAFLS
                     TASGLAMSVTGVLDQELLRPRLARFAGGDVPGVRGFRIAAVLAVVLPYLVSRVSEPLG
                     LATTVGLAFAVAAATFAPLLMLGVWWRRLSTWGAMAGLLVGGVSAGGAILITVFAPPL
                     GGWPEALLANPAMWATPLALATSIAVSLATPDRIPPGTTRTMVRLHTPERVAVRMRTR
                     D"
     gene            complement(4510..4893)
                     /locus_tag="PL014_04530"
     CDS             complement(4510..4893)
                     /locus_tag="PL014_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56388.1"
                     /translation="MSVTGGEPPQRVRVTSSRRSAAPMHARPVTRDLDEQTGLGDVYL
                     AGLMRAQLRLSLAVLGLTIGGLAALPVVLTLVPATRTLTVLWVPFPWLALGVLVYPCA
                     WFLARWYTRQAERIEADFAEVVESS"
     gene            complement(4896..5729)
                     /locus_tag="PL014_04535"
     CDS             complement(4896..5729)
                     /locus_tag="PL014_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT56389.1"
                     /translation="MSTNEAGLIALVVDDELPALSDLEYLLERDERIAEVITASSGTE
                     ALQVLDGRDVDVVFSDISMPGLDGMALARVISRFAVRPQIVFVTAHDQHAVDAFALAA
                     TDYVMKPVRTERLSEAVRRVVRTRNELASAATSPPGTTPGPGGRPGQNQPPAPPSPPE
                     DETIPVELGGVTRFITRNQIRYAQAHGDYARLHTETGSHLVRVPLSVLEERWAEHGFV
                     RIHRSTLVALPHVSEVRMDHGRCSVVVGAIELQVSRRHTKELRDTLLRRPIGDRRPSG
                     G"
     gene            complement(5752..6948)
                     /locus_tag="PL014_04540"
     CDS             complement(5752..6948)
                     /locus_tag="PL014_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT56390.1"
                     /translation="MTGSIHWPTAVLVAGTLTLLWISWLLLRRSRRRGFLSEVDRATY
                     ATLHTASLASQHLADGLTPDAAERAGRHLLSILGAHAVSLSDTGGVLSWTGVGEHHRG
                     VAFDLGDETRATGRTVVHGSTRITCDDDACPIRAAVTAPLVMDDLVVGTLSAWTEQPS
                     PGLAKATEEVAAWVSSQLALAELARTRTRVMEAELRALRAQISPHFIYNSLAAIASFV
                     RTDPDRARELLIDFADFTRYSLRSGGAFTTLAEELRNVERYLILEQARFGDRLQTSLL
                     IAPEVLPVTVPYLAVQPLVENAVRHGLAGKEGIGRLTITAADRGPDAEISIEDDGIGA
                     DPQRIRTILDGAHATDSVGLGNVDARLRQVYGDEFGLVVETAPGAGTKVSFRVPKFAP
                     GIHADG"
     gene            7023..7871
                     /locus_tag="PL014_04545"
     CDS             7023..7871
                     /locus_tag="PL014_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT56391.1"
                     /translation="MPEFDEATAVRWDAVVPAVAGATGVAHGHLDEGWAIGHAINGGV
                     LMATATSAASEVLSSVGHPDVLTWGAHFLSAARPGPLQVDLEVLRVGRGASTATVRLR
                     QPEDDGLVERVHVTGTFGTLDRAEPVHRAPAPPSMPDPDSCVPALRDSSPVATAIVML
                     DRLDVRVDPATIGFAVGRPSQRGVIRAWLRMADGREPDAALLPYVVDAFMPVSFDLGV
                     PGWAPTMELTGQVLGRPAPGWLLAELTTDTVVGDLLIEDAAVWDSAGRLVARSRQLAG
                     VRVPAN"
     gene            7881..8741
                     /locus_tag="PL014_04550"
     CDS             7881..8741
                     /locus_tag="PL014_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT56392.1"
                     /translation="MPSESPIVAAVSRLAELPGIPEQVEAAREACTRLRWHQALRKRI
                     PEAAAESRVRGAHASGELEGASVPVDVLRDVVRGAATWSPDPDPVELTMRGVLAATAE
                     TEHLAGIVLTAPLQALARLHTAAAAGLVADGALGRPRRPGEESPELTDLGAAPPAQDV
                     AVRLRDVADVLVAVPTLPVVVAAAVVHAELATVRPFVRGNAVVARALDRAVVRAGGLD
                     PTGVAVIEGGHAAGGGAAYLGALAAYGRGDAVGVGLWISHCCEAVRAAATQGEAVADA
                     VLAGRLGPPT"
     gene            9211..9945
                     /locus_tag="PL014_04555"
     CDS             9211..9945
                     /locus_tag="PL014_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56393.1"
                     /translation="MSPHAVIAVVGASGGLGASTLTLAIARRLAATGPDAVVVDLDLM
                     RGGLEVTAGVEHLPGQRWNDLGDVRGRLPPGALASRLPTEDGVHVLSARGGAGPDLSQ
                     RAVQDVLGSLAEGPARLVVDLPLGSPALPAVLGHRPLVLVLAGLRTRALADADAAVAH
                     LLDRADQAHRPVDLRLVTRGPRSAARIVDEVVAHLGIAHLHHLRDDPHVPRDAERGLF
                     PGIARDEVRRCADAVVAVVDDAAKAS"
     gene            9942..11123
                     /locus_tag="PL014_04560"
     CDS             9942..11123
                     /locus_tag="PL014_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HOT56394.1"
                     /translation="MTGIPGPFDDAVEHSIRAGLPPTTVRVAQIAAGHEGTLGTDGAA
                     SARQELLDRLVGLGPLAAVVADPMVTDVLVNGDGAVWVDRGSGVERTARMVEPEDLRP
                     LAVRLAGLAGRRLDDAQPWVDGVLPGGVRLHAVLPPLAEAGPHLSLRLARHHPEGVDA
                     LVRLGAVSHDLAVVLRQLVTRRVSLVVTGGTGAGKTTVLAGLLAEVDPSQRIVVVEDV
                     RELDPSHPHVVRLQGRVGNVEGVGEVTLVDLVRQALRMRPDRLVVGEVRGAEVRELLA
                     ALNTGHDGGMSTLHANGPQEVPARFEALGSLAGLPRHAVHAQLRQALQAVVHVERRDG
                     VRRVGVIGVLVPDPSDPGCVRVVDAVVDRAGGLRAGPGRDRLLSLLPSLATQCAWARP
                     C"
     gene            11306..11845
                     /locus_tag="PL014_04565"
     CDS             11306..11845
                     /locus_tag="PL014_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56395.1"
                     /translation="MTAVGLAAGLDLASAASVAARSPGIEAAAPWLGPRIAESVASGG
                     AVLVLEPEPAALSSQDRRDLRVLEAAWHLTEEAGAASSVVTAAAAESIRARRAATDRA
                     GVVAAGPRASMWLLSALPVAGPLAALLVGLTPGQLYGTGPAQAAAAVGLLLTALGWSW
                     SRGLLRRALRAGRTDGSVG"
     gene            11842..12462
                     /locus_tag="PL014_04570"
     CDS             11842..12462
                     /locus_tag="PL014_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT56396.1"
                     /translation="MTGWLCVGPDAGGGVMVAMAMSLAAVSVLVWPSGARWAPPAGGR
                     PGEQAPSGDGVAREQPATVEEAAAALTLVAAALRSGMGSLEALEAVARLCPDAAGREL
                     AIVAAAHRWGEASDVAWARVGPGWQTAAVSWHAAQSAGAAPAGLLAAAARRMHDDEAR
                     RVEAAVQRAGVLLVLPLGACFLPGFVATTVAPVVLLLLGGLSGARG"
     gene            12630..12836
                     /locus_tag="PL014_04575"
     CDS             12630..12836
                     /locus_tag="PL014_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HOT56397.1"
                     /translation="MATQLVRRISRLARRVRDLGEAGMTTAEYAVGTVAAVAFAGLLL
                     AVVRSGPVKSALSDIIVGALGSGA"
     gene            12863..13192
                     /locus_tag="PL014_04580"
     CDS             12863..13192
                     /locus_tag="PL014_04580"
                     /inference="COORDINATES: protein motif:HMM:NF041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HOT56398.1"
                     /translation="MATAELAVAIPSLLVVLVVALSAVDLGLSQVRCVDAARLGARLV
                     ARAEPEPVVVREALRAAPDGANVAVSRSGGFGTVTVTAPGPAVLMAIGVVGPPRATAV
                     ASLEVTS"
     gene            13189..13572
                     /locus_tag="PL014_04585"
     CDS             13189..13572
                     /locus_tag="PL014_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56399.1"
                     /translation="MKPVPQVLRRERGSATVLAVAAIAVVLSMAAAALVVTATVRDVH
                     AARGAADLSALAAAGPLAVGADPDCGAARRVAAANSARVSTCLADGAGAVVVSVTVTR
                     SVTQGWITGPGVVSARSRAGVEPLP"
     gene            complement(13605..14024)
                     /locus_tag="PL014_04590"
     CDS             complement(13605..14024)
                     /locus_tag="PL014_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56400.1"
                     /translation="MVLLGVVLVLLAVLAGVLLVTGTASIDAPVAVDLPFGTLSLPPL
                     AFLITGMVVISTFWLGWAVLRAGLRRQKRLRAQATEDAKAAQAAREADAERMKAEVEA
                     RDKQLAEERRQHEAETAALRQQAVAPRDQSSPPTPGA"
     gene            complement(14044..16341)
                     /locus_tag="PL014_04595"
     CDS             complement(14044..16341)
                     /locus_tag="PL014_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT56401.1"
                     /translation="MSAAENHLAALAAGARSERLLHVERVPARAAQVSTWPETVVPAV
                     LSALLGAGIEAPWTHQRAAIDLATAGEHVVLATGTASGKSLGYLVPVLGAASEGATAA
                     GGRGATAIYLAPTKALAADQLARIDALAVPGVRAATYDGDTPPDERRWIRDHANVVLT
                     NPDLLHHSLLPGHERWSRFLRSLRYVVIDECHVYRGVFGSHVASVIRRLRRVAARYGA
                     SPTFVLASATVSDPATHASRLVGMPVTAVTQDGSPRGALTFALWEPSTEDEQRRSATT
                     EAAQIMAELVLRGVQVVAFARSRAGAESLASSARRRIEQDDPALAAGVSAYRGGYLPV
                     ERRAIEAKLRDGSVRGLAATNALELGIDISGLDAVVLAGWPGRRASLWQQAGRAGRSG
                     EESLAVFVAADDPLDTFLVRHPEAVFGAPVEATVLDPDNPHVLGPHLAAAAAELPLTE
                     GDLAMFGPDARRLVEALVARGILRRRPTGWFWAREDRPADHISLRGVTDVVAIVEART
                     GRVLGTVDEASAHGQVHTGAVHVHQGEAWVVTELDLEARAAQVVRGDPGWSTHAQSAS
                     RFDILSTDRAVERAHVQVCFGSVRVTTQVTGFMRRLPSGEVLGTHPLDLPERSLTTKA
                     VWWTVTDEALAAAGVDEATVPGALHAAEHAAIGMLPLVATSDRWDVGGVSTALHPDTG
                     MPTIMVFDGYPGGAGFAERGFSDFEEWVAATRDAVLACACATGCPACVQSPKCGNGNE
                     PLDKAGAVAVLDLALAPAAPGVSAP"
     gene            16438..16773
                     /locus_tag="PL014_04600"
     CDS             16438..16773
                     /locus_tag="PL014_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT56402.1"
                     /translation="MDLSVSTSQQDGVAVVTVSGEVDVYTAAKLRAALDEEIAAGRNR
                     LVVDLDAVTFLDSTGLGVLVGRLKVVRNHSGWLRLVCTQDRVLRVFRITGLDTVIGIH
                     DTLEDALSS"
     gene            16898..>17633
                     /locus_tag="PL014_04605"
     CDS             16898..>17633
                     /locus_tag="PL014_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOT56403.1"
                     /translation="MAPLALASAESGSALELAGTNLTLVVTVAAIAVVALVMGFVFRN
                     QVLSADPGTEKMQEIGAAVEEGAQAFLARQFKTLSVFVVLVFGLLLLLPADTAGVRWG
                     RSGFFILGALFSAAIGYLGMSLAVKANVRVASAARGENGRDAGMKIAFRTGGVVGMAT
                     VGLGLLGAAIVVILYEGDAPKVLEGFGFGAALLAMFMRVGGGIFTKAADVGADLVGKI
                     EAGIPEDDPRNAATIADNVGDNVGDCA"
BASE COUNT         2329 a         6125 c         6704 g         2475 t
ORIGIN      
        1 gacatggcat ccatccgggc gccgagggct tcagcggcgg ggtcgaaggt ccggccggcc
       61 cagcgcgcgt agatgaacgt gatggcgaac gtcgtgacga actgcagcag gccgaacacc
      121 aggcccacgg tgaccaggcc gctgatcggc ttgttcatga agccgggggc gaagatcgac
      181 agcagcacgt acaggaagta ccaggtgagg aagaacgcgg tcatcgggaa gacgaagccg
      241 cggaacttct tacgaaggtt ctggaactca tcggtctctt gcagcgcgac gtaggggtcg
      301 tcggcgcctg ggcgagcttc gacgctcacg gccacctcct gggtctgccc gggcatcgtt
      361 gctcgggatc gagtcccaga gtgggcgcgc ccggatgccg ttgtgcaccc cccgttgcga
      421 cccgcgcggc gggcgggtgg ctggcttcgg tgtgcggcgg ggcgctgcgc ggcgaacggc
      481 gcgcgtcgcg cgacgaacgg tgacccgccg gcatcccacg cggcatgatg gcgcaatgcg
      541 cgcactgcgg aaggtcaccg ccggccccgg tctggaactc gtcgagattc ccgaaccgga
      601 gtgtggcgac aaggacgtca agatcagggt gctgcgagcg ggcctgtgcg gcaccgacct
      661 gcacctcgag cagtgggacg agtgggcggc ggccacggtg cgcgccccca tgacgctcgg
      721 gcacgagttc ttcggcgagg tcgtcgaggt cggggccgac gtcgaccacg tggccgtcgg
      781 tgaccgggtc tcgggggagg ggcacctggt ctgcggcacc tgccgcaact gccgggccgg
      841 gcgccgtcac ctgtgcatca acaccgtcgg cgtcggtgtc aaccgcgacg gcgcctttgc
      901 cgactacgtc gtcatcccgg cgacgaacgc gtgggtgcag ccgcccgacc tggaccccga
      961 cctcggggcg ctgttcgacc cgctcggcaa cgccacgcac acggcgctgc agtggccggt
     1021 cgtcggtgag gacgtcgtca tcaccggggc cgggccgatc ggggtcatgg cgacggcggt
     1081 cgtgcgccac gcgggggcgc gccggatcgt cgtgaccgac atgtcggccg ggcgcctggc
     1141 gctggcagcc gcggcggggg cggacctcgt cgtcgacgtg tccgcaggcc agacgttgcg
     1201 cgacgcccag ggccagctcg gcatggcgga ggggttcgac atcgccctcg agatgtccgg
     1261 gtcacccgcg gccgtcgagg acctgctcgc gaacatgaac cacggcggcc gcgtcgcgat
     1321 gctcggcctg cccaaggacc gctacgccat cgactggggc cgggtcatca cgcacatgat
     1381 caccctgcgc ggcatctacg ggcgcgagat gttcgagacc tggtacctca tgggctcgat
     1441 gctcgcgacg tcgcccgagc tgcgcacggc ggtgtcgtcg gtggtgacgg ggcggtacgc
     1501 ggcggccgag tggcaggccg cgttcgacga gacccggtcg ggcaccggcg gcaaggtcgt
     1561 gctcgactgg agctgagggt gaccacggat gccggggggc ggcacccgtg accgcacacg
     1621 gcatacgctg cacgacagag gcggcgcagc cccgcgctgc cgctgacgac accacgctgg
     1681 aggcgacgtg tacggcaccg tcaaggacga gctcgcggcc accctcaagg agatcgagga
     1741 cgccggcctg tacaagcgcg agcgtgagct cacgacgccg caggccgctc acgtcgccac
     1801 cgcgggcggg tcgtcgctga acttctgcgc caacaactac ctggggttcg ccgaccaccc
     1861 cgacgtcgtg gctgccagcc gaaacgccct ggacgagtgg ggtttcggca tggcctcggt
     1921 gcgcttcatc tgcggcaccc agacccagca cacgctgctc gaacagcggt tgtcgcagtt
     1981 cctcggcatg gaggccacca tcctgttcgg ctcgtgcttc gacgccaacg gcggggtgtt
     2041 cgaggtgctc ttcggcgccg aggacgcgat cgtgtccgac gagctgaacc acgcgtcgat
     2101 catcgacggc atccggctca gcaaggccgc ccgcttccgg tacaagaacg ccgacatggg
     2161 tgacctgcgg gcccagctcg aggcggcccg ggcgggcggg gcgcggcgcg tgtgcgtcgt
     2221 caccgacggg gtcttctcga tggacgggtc gtacgccccg ctcgaccaga tctgcgacct
     2281 ggccgacgag ttccaggcga tggtgctggt cgacgactcg cacgcggtgg gcttcgtcgg
     2341 tgaggggggc cggggcacgc ccgagctgtt cggggtcatg gaccgcgtcg acatcatcac
     2401 cggcaccctc ggcaaggccc tcgggggcgc ctcgggcggg tacgtgacga gccaccagga
     2461 ggtcgtcgac ctgctgcgcc agcgagcccg gccgtacctg ttctcgaact cggtggctcc
     2521 cgcggtggtg gccggctcgc tggcagcgct cgacctcgtg tcgggctcga gcgaggcccg
     2581 cgagaccctg cgtgcgaact cggccctctt ccgtgagctc atgacggatg ccgggttcga
     2641 gctgttgccc ggatcgcacc cgatcacccc ggtcatgttc ccgggtgagg acggtgctcg
     2701 gcaggctgcc gaggtggccg accacatgct cgcggccggg gtctacgtcg tcgccttcag
     2761 cttccccgtg gtgccgcgcg gcagggcgcg catccgggtg cagttgtcag cggcccactc
     2821 ggaggccgac gtccgtgagt gcgtcgacgc gttcgtggcc gcgcgagcgg ccgtcggctg
     2881 acggcatccg cccgtcgaag gtagacgaat cgccgtctga gcgtccgccc ttgcggcgat
     2941 tcgcctcctc tcgactgctg ggggggcggg gcggtcggag ttctcagtcg cgggtgcgca
     3001 tcctcacggc gacgcgctcg ggggtgtgca gccgcaccat ggttcgggtc gtcccgggcg
     3061 ggatgcggtc cggggtcgcg agcgagacgg cgatcgaggt cgcgagggcc agcggggtcg
     3121 cccacatcgc ggggttcgcg agcagcgcct cgggccagcc gccgagcggt ggcgcgaaca
     3181 cggtgatgag gatcgcgccc ccggcgctga ctcctccgac gaggagcccg gccatcgcgc
     3241 cccacgtcga cagccggcgc caccacacgc cgagcatgag cagcggggcg aacgtcgccg
     3301 cggcgacggc gaaggccagc cccaccgtgg tcgcgagccc gagcggctcc gacacccggc
     3361 tcacgaggta gggcagcacc acggccagca ccgccgcgat ccggaaacca cggacaccgg
     3421 gtacgtcacc accggcgaag cgggcgagtc gtgggcgcag cagttcctgg tcgaggacgc
     3481 cggtcaccga catcgccagg ccggatgccg tggacaggaa cgcggcgaac gcgccccccg
     3541 cgagcatcgc ggtgagcagc tgcccggcca gcccgtcgac catggtctcg ggtagttgga
     3601 gcacgatcgt gtccgagccc atgccgtcgg gcagggtcgg gtaggcgatc cggcccagtg
     3661 ccccgtagac gggcgggatg acgtagaacg ccccgagcag cgccagcacg gtgacggtgg
     3721 tgcggcgggc accgttgccg tcggggttcg tgtagtagcg gacgaggacg tggggcaggc
     3781 ccatggtccc gaggcacagg gcgagaacgg tggatgccgt gcggtaccac gggtggtcgt
     3841 cgggcccggt ggggcgcagc ggcaggtgcc acgacgggtc gacctggggc gcaggtgacc
     3901 ccgaccggaa ccacagcgcc agcaggacga acgccggcac tgccagtgcg gtgagcttga
     3961 tccagtactg gacggcctgc acgaaggtga tcgaccgcat cccaccagcg ccgacggcga
     4021 gggcgatcac gaccatgacg atgaacccgc cgacccacgt gggggccccc gtgacgaggg
     4081 tgagggtcag gctcgctccc tggaactgcg gcacgaggta gagccagccg atggcgatga
     4141 ccagcgtcga cgacacgagc cggatcgcgc gcgaccccag ccgggcctcg gcgaagtcgg
     4201 gcagcgtgta ggcacccgac cggcgcatcg gggcggcgac gacgacgagc aggacgaggt
     4261 atcccagcgt gtagcccacg ggcagccaca gcatgtcgac accgcggtcg tagaccaggc
     4321 cggccacgcc gaggaagctg gcggccgaca ggtactcccc gccgatggcc gaggcgttca
     4381 tccgtggcga gaccgtgcgg ccggcgacgt agaagtcgct cgtgcggcgc gacatccgca
     4441 gcccgaggcc accgacgatg agcgtggtga ggcacacgag cgccaccgcg gcgacggaga
     4501 gggggttgtt cacgacgact cgacgacctc ggcgaagtcg gcctcgatcc gctcggcctg
     4561 acgggtgtac cagcgggcga ggaaccacgc gcacgggtag accagtactc cgagggccag
     4621 ccaggggaag ggcacccaga gcacggtgag ggtccgggtc gccgggacca gggtgaggac
     4681 gacgggcagg gcggcgagcc cgccgatggt caggccgagc acggcgagcg acagcctcag
     4741 ctgggcccgc atcaaccccg cgaggtagac gtcgccgagg ccggtctgct cgtcgaggtc
     4801 acgggtgacg gggcgggcgt gcatcggtgc ggcgctgcgg cgcgagctcg tcacccgcac
     4861 gcgttgcggt ggctccccgc cggtcacgct cacgctcagc ccccggacgg gcgccggtcg
     4921 ccgatcggcc gccgcagcag cgtgtcgcgc agttccttgg tgtggcggcg gctcacctgc
     4981 agctcgatgg cgcccacgac gaccgagcac cggccgtggt ccatgcgcac ctcgctcacg
     5041 tgggggaggg cgacgagggt gctgcggtgg atgcgcacga acccgtgctc ggcccagcgc
     5101 tcctcgagca cgctgagcgg cacgcgcacg aggtgcgacc cggtctcggt gtgcagccgg
     5161 gcgtagtcgc cgtgggcctg ggcgtagcgg atctggttgc gggtgatgaa ccgggtgacc
     5221 ccgcccagct cgaccgggat cgtctcgtcc tccggtgggg acgggggtgc cgggggctgg
     5281 ttctggccgg ggcgcccgcc gggaccgggc gtcgtgcccg gcggcgaggt ggccgcggac
     5341 gccagttcgt tgcgggttcg cacgaccctg cggaccgcct cggagagccg ttcggtgcgc
     5401 accggcttca tgacgtagtc ggtggctgcg agggcgaacg cgtcgacggc gtgctggtcg
     5461 tgggcggtga cgaagacgat ctgcgggcgc acggcgaacc gcgagatcac ccgggccagg
     5521 gccatcccgt cgagccccgg catcgagatg tcgctgaaca cgacgtcgac gtctcgcccg
     5581 tcgaggacct gcaacgcctc ggtgccactg gatgccgtga tgacctccgc gatgcgctcg
     5641 tcgcgctcga ggaggtactc gaggtcggac agcgcgggga gctcgtcgtc gacgacgagt
     5701 gcgatgagcc cggcctcgtt ggtggacatg gcggcagcct attgccctcg tctagccatc
     5761 tgcgtggatg ccgggtgcga acttcggcac acgaaagctc accttcgtgc ccgctcccgg
     5821 ggcggtctcg acgacgagcc cgaactcgtc accgtagacc tgtcgcagac gggcatcgac
     5881 gttgccgagg ccgaccgagt cggtggcgtg ggccccgtcg aggatggtgc ggatccgctg
     5941 gggatcggcg ccgatgccgt cgtcctcgat ggagatctcg gcgtcggggc cgcgatctgc
     6001 cgcggtgatg gtcagccggc cgatcccttc cttgccggcg agcccgtgcc gcacggcgtt
     6061 ctcgaccaag ggctgcacgg cgaggtaggg aacggtcacc ggtagcactt cgggcgcgat
     6121 gagcaacgag gtctggagcc ggtcgccgaa gcgggcctgt tcgaggatga ggtagcgctc
     6181 gacgttgcgc agctcttcgg ccagcgtcgt gaacgcgccc cctgagcgca gcgagtagcg
     6241 ggtgaagtcg gcgaagtcga tgagcagttc gcgggcgcgg tcggggtcgg tgcgcacgaa
     6301 cgacgcgatc gccgcgaggc tgttgtagat gaagtgcggg ctgatctgtg cgcgcagggc
     6361 ccgcagttcg gcctccatga cccgggtgcg agtgcgcgcg agctccgcga gcgcgagctg
     6421 gctggagacc caggcggcga cctcctccgt ggccttggcg agccccggtg acggctgctc
     6481 ggtccaggcg ctcagggtcc cgacgacgag gtcgtccatg acgagagggg cggtgaccgc
     6541 agcccggatc gggcaggcgt cgtcgtcgca ggtgatccgg gtcgagccgt gcacgaccgt
     6601 gcggccggtg gcccgggtct cgtcgccgag gtcgaaggcg accccgcggt ggtgctcgcc
     6661 gacgcccgtc caggagagca cccctccggt gtcggacagg gagacggcgt gcgcgccgag
     6721 gatggagagc aggtgccgac cggcgcgctc agcggcatcc ggggtgagtc cgtccgcgag
     6781 gtgctgggac gccagggatg ccgtgtggag ggtggcgtac gtggcgcggt cgacctcgga
     6841 gaggaacccg cgtcgccggc tgcgtcgcag cagcaaccag gagatccaca acagggtcag
     6901 ggtgcccgcg acgagcaccg ccgtcggcca gtggatgctc cctgtcatgc gctcaccata
     6961 gggtcgagcc cagccgacgg gacaggtggt cgcgacggcc cagggaccag ggaggcgcag
     7021 tgatgccgga gttcgacgag gcgacagccg tcaggtggga tgccgtggtg cccgcggtcg
     7081 cgggcgcgac cggagtggcc cacgggcacc tcgacgaggg gtgggccatc gggcacgcca
     7141 tcaacggtgg ggtgctcatg gcgaccgcga cgtccgcggc atccgaggtc ctgtcgtccg
     7201 tggggcaccc ggacgtgctc acctggggcg cgcacttcct ctcggcggcc aggccgggcc
     7261 cgctccaggt ggacctcgag gtgctgcggg tgggccgcgg cgcgtcgacg gcgacggtgc
     7321 gcctgcgcca accggaggac gacgggctcg tcgagcgggt gcacgtcacc ggcaccttcg
     7381 gcaccctcga ccgggccgag ccggtccacc gtgctccggc cccgccgtcg atgcctgacc
     7441 cggactcctg cgttcccgcg ctccgcgact cgtcaccggt cgcgacggcc atcgtcatgc
     7501 tcgaccgcct cgacgtgcgg gtggaccccg ccaccatcgg cttcgcggtc ggccggccca
     7561 gccagcgtgg ggtcatccgc gcctggttgc ggatggccga cggtcgcgag ccggatgccg
     7621 cgctgctgcc ctacgtcgtc gacgccttca tgccggtgtc cttcgacctc ggggtgcccg
     7681 gctgggcgcc gacgatggag ctcacgggac aggtgctcgg ccgaccggcg ccggggtggt
     7741 tgctcgccga gctcacgacc gacaccgtgg tgggtgacct gctcatcgag gatgccgcgg
     7801 tgtgggactc cgcgggtcgt ctcgtggcgc gttctcgcca gctcgctggc gtgcgggtgc
     7861 ccgcgaacta gtctgcacgg gtgccttccg agagcccgat cgtcgccgca gtctcccgac
     7921 tggccgagct gcccggcatc cccgagcagg tcgaggccgc ccgtgaggcc tgtacccgcc
     7981 tgcggtggca ccaggccctg cgcaagcgga tccccgaggc cgcggccgag tcccgcgtgc
     8041 gaggtgccca cgccagtggc gagttggagg gcgccagcgt gccggtcgac gtcctgcgcg
     8101 acgtggtgcg gggtgcggcc acctggtcac ccgatcccga cccggtcgag ttgacgatgc
     8161 ggggggtgct ggcggcaacc gcggagaccg aacacctggc gggcatcgtg ctcacggcgc
     8221 cgctccaggc gttggcccgg ctgcacacgg ccgcagcagc ggggctggtg gccgacggtg
     8281 ccctcgggcg gccacgacgg cccggcgagg agtcccccga gctgaccgat ctcggtgccg
     8341 ccccgccagc ccaggacgtg gcggtacggc ttcgtgacgt cgcggacgtg ctggtggccg
     8401 tgccgacgct gcccgtggtc gtggccgcgg cggtggtgca cgccgagctc gcgaccgtgc
     8461 ggccgttcgt gcgcggcaac gccgtggtgg cgagggcgtt ggaccgggcc gtggtgcggg
     8521 ccggcgggct cgatccgacc ggggtggccg tcatcgaagg cggtcatgcg gcaggtgggg
     8581 gagcggccta cctcggggcg ctcgccgcct acggccgagg ggatgccgtc ggtgtggggc
     8641 tgtggatctc gcactgttgt gaggcggtgc gcgcggcggc cacccagggc gaggcggtcg
     8701 ccgatgcggt gctggcgggg cgcctgggcc cgcccacctg agcgtcgcga tcgcgccgaa
     8761 tcgggctggg gccgagggat ctcggtggcc cgtgcgctcc cttgacacgg acctcgtcat
     8821 cgatcagcgc cacgcaggtc gtagagcgtt aaacagtttg ggcaaaacct tcggattcag
     8881 tggacctgag gatgtgctga aaatgttctc gatagttctg accaagggct tgcgtcggag
     8941 tccggtcgta gagcacgata tgactactcg ctccccttcg gggtcgagcg aacggcgaac
     9001 cggcttctcc cccccggagc tcggctcgcc gacggccccg gttgtccccc cgaccggggc
     9061 cgtcccatgt ccggaaacaa gtggcgcacc agcggtcaga cgatgggcgc accaggggtc
     9121 gttgtccaca gccagccgac ggcgcgatgg ttgtccacag ggtgtccgcc gcgcctcgcg
     9181 tgaccacgtg atcccggcga gggtcggtgc atgtcaccac acgccgtcat cgccgtcgtc
     9241 ggggcgtccg ggggcctggg cgcgagcacc ctgaccctcg ccatcgcccg ccgtcttgcc
     9301 gcgacaggcc ccgatgccgt cgtcgtcgac ctcgacctga tgaggggagg gctggaggtg
     9361 actgcgggcg tcgagcacct gcccgggcaa cgatggaacg acctgggtga cgtgcgtggg
     9421 cgcctgccgc cgggtgccct tgcgtcccgg ttgccgaccg aggatggcgt gcacgtgttg
     9481 tcggcccgcg gtggggccgg ccccgacctc tcgcagcggg ccgtccagga cgtcctcggc
     9541 tctctggccg agggccccgc ccggctggtc gtcgacctgc cgctggggag cccagcactc
     9601 cccgcggtgc ttggtcaccg gccactggtg ctcgtgctgg ccggcctgcg cacccgtgcc
     9661 ctggccgatg cggatgccgc cgtcgcccac ctgctggatc gggccgacca ggcccaccga
     9721 cccgtggacc tgcgcctggt cacccgaggc ccccgcagcg ccgctcgcat cgtcgacgag
     9781 gtcgtcgcgc acctcggcat cgcccacctg caccacctgc gcgacgaccc ccacgttccc
     9841 cgcgacgcgg agcgaggact gttcccgggg atcgcccgcg acgaggtgcg acggtgtgcc
     9901 gacgccgtgg tcgcggtcgt cgacgacgcc gcgaaggcgt catgaccggc atcccagggc
     9961 ccttcgacga cgctgtcgag cactccatcc gggcgggtct cccgccgacc accgtgcggg
    10021 tcgcccagat cgccgcgggc cacgagggaa ccctcggcac cgatggcgcg gcatccgccc
    10081 gtcaggagct gctcgatcga ctcgtcggac tggggccgct cgcagcggtc gtggccgatc
    10141 ccatggtgac cgatgtcctc gtcaacgggg acggggcggt ctgggtcgat cgggggtcgg
    10201 gcgtggagcg cacagcgcgg atggtggaac ccgaggacct gcgccccctc gccgtgcggc
    10261 tcgccggcct ggccggtcgg cggctcgatg acgcccaacc gtgggtcgac ggtgtgctgc
    10321 ctgggggtgt gcgtctccac gcggtcctcc cgccgctcgc ggaggcgggc ccacacctca
    10381 gcctgcggct ggccaggcac caccccgagg gtgtcgacgc gctcgtgcgg ttgggcgcgg
    10441 tgagccacga cctcgcggtg gtcctgcgcc agctcgtgac ccgcagggtc tccctcgtcg
    10501 tgaccggcgg caccggggcc ggcaagacca ccgtcctcgc cgggctgctc gccgaggtcg
    10561 acccgtccca gcggatcgtc gtggtcgagg acgtccgcga gctcgacccc agccatcccc
    10621 acgtcgtccg gctgcaaggg cgagtgggga acgtcgaagg ggtcggagag gtgacgctcg
    10681 tcgacctggt gcgacaggcc ctgcggatgc gccccgaccg cctcgtcgtc ggcgaggtgc
    10741 gcggtgccga ggtgcgcgag ctgctcgccg ccctcaacac cgggcacgac ggggggatga
    10801 gcacgctgca cgcgaacggc ccgcaggagg tccccgccag gttcgaggcg ctcggttcgc
    10861 tggcggggct gccacgccat gccgtgcacg cccaacttcg ccaggcactc caggcggtgg
    10921 tgcacgtgga gcgccgggac ggtgtgcgcc gtgtcggcgt catcggcgtg ctggtgcccg
    10981 acccgtcgga ccctggctgt gtccgggtgg tcgacgcggt cgtcgaccga gccggggggc
    11041 tgcgagcggg gcctggccgt gaccgactgc tgagcctgct gccctcactc gcgacacagt
    11101 gcgcctgggc ccggccgtgc tgatcgctgc gctgctggca gccctcgcgg tgtgttgctg
    11161 gccggggcga tggagccgag ccacagcgga tgccgcggcg cggggtgagc ctgtcggggg
    11221 cggaacgtgg gggcggtggc gcgcccgggt ggccggtggg gtgcggcggg ggcgtcagcc
    11281 accgtgggtt gccgacctcg ccgaggtgac ggcggttgga ctcgcggccg ggctcgacct
    11341 ggcctccgcg gcgtcggtcg cagcccggtc gcccggcatc gaggcggcgg ctccgtggct
    11401 ggggccgcgc atcgccgagt ccgtcgcctc cggtggggcc gtcctcgttc tcgaacccga
    11461 gccggccgcc ctgtcatcgc aggatcgtcg cgaccttcgt gtcctcgagg cggcctggca
    11521 cctcaccgag gaggcgggag ccgcgtcatc ggtggtcact gcggccgccg ccgagtcgat
    11581 tcgggcgcga cgggcggcaa cggatcgggc aggcgtcgtc gccgcgggtc cgcgggcctc
    11641 catgtggctg ctctcggccc tgccggtcgc agggccactc gccgccctgc tcgtcgggct
    11701 cacccccggc cagctctacg gaaccggacc ggcgcaggcg gcagcggccg tcggtctgct
    11761 gctcaccgcg ctcggctggt cctggtcgcg aggcctgctt cggcgggcac tgcgggcagg
    11821 ccgcacggac gggagcgtgg ggtgacgggc tggctctgcg tggggccgga tgcgggtggg
    11881 ggcgtcatgg tggccatggc gatgtcgctc gccgcggtgt cggttctcgt ctggccgtcg
    11941 ggagccaggt gggccccgcc cgcaggaggc aggccgggcg aacaggcgcc gagcggggac
    12001 ggcgtggctc gcgagcagcc ggccaccgtg gaggaggctg cggcagcact gaccctcgtc
    12061 gcagcagccc tgcgcagcgg catgggctcg ctggaggcgc tcgaggccgt cgcccggctc
    12121 tgccccgatg ctgctggtcg cgagctggcg atcgtcgcgg cggcgcaccg ttggggggag
    12181 gccagtgacg tcgcgtgggc gcgggtgggc ccggggtggc agacggcggc ggtctcgtgg
    12241 cacgccgccc agagcgccgg tgcggccccg gccgggctgc ttgccgccgc ggctcggcgg
    12301 atgcacgacg acgaggcccg ccgggtcgag gcagccgtgc agagagcggg ggtgttgctc
    12361 gtcctgccac tcggggcctg tttcctcccc ggattcgtcg cgacgacggt ggcgcccgtc
    12421 gtgctgctgc tgctcggcgg gctcagcggc gcccgtggct gagtggtgcc gccatcgccg
    12481 acggggccgg gggcggctgt ccacaggcgg gcacggacgg ggcggtcgtc cacagggtcg
    12541 tcggggcgat cagcgcgtac gggccgagtc gcgaagagtg ctcaccaggg ggcgcgagac
    12601 acgcgctcgt ggatgcagag gaggtcgcga tggcgacaca actggtacgc aggatcagcc
    12661 gactggcgcg acgggtccgt gacctgggcg aggcgggcat gacgacggcc gagtatgccg
    12721 tcggcacggt ggccgctgtg gccttcgccg ggttgctcct ggctgtggtg cggtcagggc
    12781 cggtcaagag cgccctgtcc gacatcatcg tcggggcgct cggcagcggg gcctgaccgt
    12841 gcggcgtcgg cgtgagcgcg ggatggcgac cgccgagttg gcggtggcga taccgagcct
    12901 gctcgtcgtc ctggtggtgg cgttgtctgc cgtcgacctg ggcctgtcgc aggtgcgctg
    12961 cgtcgacgcc gcccggctcg gggcgcgtct ggtggccagg gccgagcccg agccagtggt
    13021 ggtcagagag gctctgcgtg ctgctcctga cggggcgaac gttgcggtga gccgcagcgg
    13081 tgggttcggc accgtgacgg tcaccgcgcc cgggcccgcc gtgttgatgg cgatcggcgt
    13141 ggtcggtcca cctcgtgcga ccgccgtcgc gtcgctggag gtgacctcgt gaagccagtg
    13201 ccgcaggtac tgcggcgaga gcggggaagt gccacggtcc tcgccgtcgc ggccatcgcc
    13261 gtggtgctgt ccatggccgc agcagccctg gtcgtgacgg ccaccgtgcg tgacgtccat
    13321 gcggctcgag gtgccgccga cctgtcagcc ctcgctgccg ccggcccgct cgccgtcggc
    13381 gccgaccccg actgcggcgc ggcccggcgg gtggccgccg ccaactcggc gagggtgtcg
    13441 acgtgcctcg ccgatggtgc aggcgcggtc gtggtgtccg tgacggtcac gaggtcagtg
    13501 acccaaggtt ggatcaccgg ccccggcgtc gtctccgcac ggtcgcgggc aggtgtcgaa
    13561 ccgttgccgt gagcgggctc ccctgtgggg agagcccggg gtgttcaggc ccccggcgtg
    13621 ggcgggctgc tctggtcgcg aggtgccacg gcctgctgac gcaacgccgc ggtctcggcc
    13681 tcgtgctggc gccgctcctc ggcgagctgc ttgtcgcgcg cctcgacctc ggccttcatc
    13741 cgctcggcgt cggcttcgcg tgctgcctgg gcggccttgg cgtcctcggt ggcctgggca
    13801 cgcagccgct tctggcgccg cagacctgcg cgcagcaccg cccagccgag ccagaaggtc
    13861 gagatcacga ccatgccggt gatgaggaag gccagcgggg ggaggctgag ggtgccgaag
    13921 ggaaggtcca ccgcgaccgg ggcatcgatc gacgccgttc cggtgacgag aagcacgcca
    13981 gcgaggacgg caagcaggac gagaacaacc ccgagaagca ccatggcggc gactgtaccc
    14041 acctcagggc gcggagacgc ccggagcagc aggcgcaagc gcgaggtcca gaacagcgac
    14101 ggcaccggcc ttgtcgaggg gttcgttgcc gttgccgcac ttcggcgact gcacgcacgc
    14161 cgggcacccc gtggcgcacg cgcaggcgag caccgcatcg cgggtcgcgg ccacccactc
    14221 ctcgaagtcc gagaacccgc gttcggcgaa tcccgccccg cccgggtagc cgtcgaacac
    14281 catgatcgtc ggcatccccg tgtccgggtg cagagccgtc gagacaccgc cgacgtccca
    14341 ccggtccgag gtggcgacca gcggcagcat cccgatggcg gcgtgctcgg ccgcgtgcag
    14401 cgccccgggg acggtcgcct cgtcgacacc tgccgccgcg agcgcctcat ccgtcactgt
    14461 ccaccacacg gccttggtgg tcagtgatcg ttccggcagg tcgagcgggt gggtgccgag
    14521 cacctcaccc gacggcaggc gcctcatgaa cccggtgacc tgggtcgtca cccgcacgct
    14581 gccgaaacag acctgcacgt gagcgcgctc caccgctcgg tcggtggaga ggatgtcgaa
    14641 gcggctcgcc gactgcgcgt gcgtggacca cccggggtca ccgcgcacca cctgggcggc
    14701 ccgggcctcc aggtcgagct cggtcaccac ccacgcctcg ccctggtgca cgtgcacggc
    14761 ccccgtgtgc acctgcccgt gggccgacgc ctcgtcgacg gtgccgagga ccctgccggt
    14821 gcgcgcctcc acgatcgcga cgacgtcggt gaccccccgc agcgagatgt ggtcggcggg
    14881 gcggtcctcg cgtgcccaga accagccggt cgggcggcga cgaaggatgc cgcgggccac
    14941 cagggcctcg acgaggcggc gggcgtcggg cccgaacatc gccaggtcgc cctcggtgag
    15001 ggggagctcg gccgcggcgg ccgccaggtg aggccccagc acgtgcgggt tgtcgggatc
    15061 gagcacggtc gcctcgaccg gggcgccgaa cacggcctcg ggatgccgga cgaggaaggt
    15121 gtcgagtggg tcgtcagccg cgacgaacac ggccagtgac tcctcgcccg agcggccggc
    15181 acgccccgcc tgctgccaca ggctcgcccg gcgacccggc caccccgcca gcacgacggc
    15241 atccaacccg ctgatgtcga tgccgagctc gagcgcgttc gtggcagcca ggccacgcac
    15301 cgacccgtcg cgcagctttg cctcgatggc tcgacgctcc acggggaggt agccgccgcg
    15361 gtacgccgac accccggcgg ccagggccgg gtcgtcctgc tcgatgcggc gtcgcgcgct
    15421 gctcgcgagc gactccgccc cggcccggct gcgggcgaac gcgaccacct gcacgccgcg
    15481 caggacgagc tcggccatga tctgcgcggc ctcggtcgtc gccgaccggc gctgctcgtc
    15541 ctcggtcgac ggttcccaca acgcgaaggt gagggcaccg cgaggcgagc cgtcctgcgt
    15601 gacggccgtg accggcatcc ccacgaggcg cgacgcgtgg gtcgcggggt cgctcacggt
    15661 cgcggacgcg aggacgaacg tcggggacgc gccgtaccgg gccgcgaccc gccgcagccg
    15721 gcggatcacc gacgccacgt gggagccgaa caccccgcgg tagacgtggc actcgtcgat
    15781 gacgacgtaa cgcagcgacc gcaggaaccg cgaccagcgc tcgtggcccg gcaggaggga
    15841 gtggtggagc aggtcggggt tggtgagcac gacgttcgcg tggtcccgga tccaccgccg
    15901 ctcgtcgggc ggggtgtcgc cgtcataggt cgcggcccgc accccgggca ccgcgagggc
    15961 gtcgatgcgc gctagctggt cggccgcgag cgccttcgtg ggggcgaggt agatggcggt
    16021 cgcgccgcga cctcctgcgg ccgtggcgcc ctcgctcgca gccccgagca ccgggacgag
    16081 gtagcccagg ctctttccgg atgccgtacc cgtcgccagc acgacgtgct cacccgcggt
    16141 cgcgaggtcg atggcggccc gttgatgggt ccacggcgcc tcgatcccgg cgccgagcag
    16201 ggccgagagc accgcgggca caaccgtctc aggccacgtc gacacctggg ccgcccttgc
    16261 cggcacccgc tcgacgtgca gcagtcgctc gctgcgtgcc cccgctgcaa gtgctgcgag
    16321 gtggttctca gcggcggaca tgccggttac ggtaggcggt gcctccgacc cccatcagca
    16381 gccctgccgc gtgacgagtc cgtggacagc ggcaacgacc gaaagtggtg cctccccgtg
    16441 gacctctccg tgagcacctc ccagcaggac ggtgtcgctg tcgtcaccgt gagtggcgag
    16501 gtcgacgtct acaccgcggc caagctgcgt gcagccctcg acgaggagat cgccgccggc
    16561 cggaaccggc tcgtcgtcga cctcgatgcc gtcaccttcc tggactccac cggcctcggc
    16621 gtgctcgtcg ggcgcctgaa ggtcgtgcgc aaccactcgg gctggcttcg gctcgtctgc
    16681 acccaggacc gggtgctgcg ggtcttccgg atcaccgggt tggacaccgt gatcggcatc
    16741 cacgacacgc tcgaggacgc gctctcctcc tgagggtgac ctgagtcact ccggcgtcgg
    16801 ggtctgccga cggcgtgtcg gccggtaagg tgcgcccggg cgtcctgccg tcggcgtgac
    16861 tcgccccggg acgcacaaac ccgtcaagga ggatggaatg gcaccgcttg cgctcgcttc
    16921 ggctgagtca ggctcggccc tggaactcgc tgggacgaac ctcacgctcg tcgtcaccgt
    16981 cgcggccatc gcggtcgtgg cgctcgtcat ggggttcgtc ttcaggaacc aggttctgag
    17041 cgccgacccc ggcaccgaga agatgcagga gatcggcgct gccgtcgaag agggcgcaca
    17101 ggccttcctc gcgcggcagt tcaagaccct cagcgtcttc gtcgtcctgg tcttcggcct
    17161 gctgctcctg cttccggccg acacggccgg tgtgcggtgg gggcgctcgg gcttcttcat
    17221 cctcggcgcg ctgttctccg cggcgatcgg gtacctcggc atgagcctcg ccgtcaaggc
    17281 caacgtccgc gtggcctcag ccgcccgtgg cgagaacggc cgcgacgccg gcatgaagat
    17341 cgccttccgc accggtggcg tcgtcggcat ggcgacggtc gggctgggcc tgctcggcgc
    17401 agccatcgtc gtcatcctct acgaaggtga cgcacccaag gtcctcgagg gcttcggctt
    17461 cggtgccgcg ctgctcgcga tgttcatgcg cgtcggcggc ggcatcttca ccaaggccgc
    17521 ggacgtcggc gccgacctcg tcggcaagat cgaggccggc atccccgagg acgacccgcg
    17581 caacgccgcc acgatcgccg acaacgtcgg tgacaacgtc ggtgactgcg ccg
//
LOCUS       DAOSOC010000035        17187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3348_length_17187_cov_9.276967, whole genome shotgun sequence.
ACCESSION   DAOSOC010000035 DAOSOC010000000
VERSION     DAOSOC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 17187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17187
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3348_length_17187_cov_9.276967"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1135
                     /locus_tag="PL014_04610"
     CDS             119..1135
                     /locus_tag="PL014_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT56404.1"
                     /translation="MAGRSNDPAGPASPNAAGEAASPVARTGQSSYPIEDGRDGPGPA
                     PYVTIVLPCYNEGAHVLKEIDRITAAMTASELTYELLCIDDASTDDTLDVLRHAQQNY
                     PNLRILPFRRNGGSGTARRIGTQQARGEIVVWTDADMTYENERIPELVRILRDDASYD
                     QVVGARTTEEGTHKWARVPAKWLIRNIAQWLTKTKIPDLNSGLRAFRREVSLPYLRLL
                     PAGFSCVTTITIAFLANQHDIKYVETSYAKRAGVSKFHFVGDAYRYILQVLRMVMYFD
                     PLKVLMPPALWMIVLGVGKAVVDMVRHPFYFPASTVLLIVSGIMIASLALLSDLVVRS
                     RDGV"
     gene            1128..3056
                     /locus_tag="PL014_04615"
     CDS             1128..3056
                     /locus_tag="PL014_04615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT56405.1"
                     /translation="MSERDASSGSAALTVALVGPTHPFKGGVAAHTTTLAHELSEAGH
                     EVTLVSWSHLYPSALYPGEQSVPGGAPDVEPFPRTLRVLSWARPDTWLRTGRRLRDVD
                     VIVVVHVIPAVVPAHLALLRAAGAGRRTASGHGPRTILIAHNVLPHESHPGARQLVES
                     LVERVDAVVVHSDEQARLATTLGAHHVRRLDLPPHLPGGDPEPRVANDADTASTPVRV
                     LALGIVRDYKGVDLLVDAVRDVPGIHLTIAGELWGDAGRTVRDLANDPRVAGRVELHP
                     GYVPADRIAGLLAQHDVMALTYRTATASQNVLLAHKHGLPVLATTVGTFPEQIRDGVD
                     GLLVPPGDRGALVEALRRLAEPGVTEALRSAVRPPDLSGPWANYVGGIEALAAAPLGP
                     VDEADSEGGGAGLRLGPVARAKATVAGIRARRRPVVEIAPGDIPSRVRATDVLALDSD
                     AVDAVAIARELGLPRPGEPVAAWAALGALAAVVRIRDDGHRSSVIVDESGSGSPLGRW
                     ARAVGFAPVELELTGQRAAVAVLDIDTASLDVITRLHPGGCTASDVDVAIGQASWALR
                     SGGLLILTLPIGDDDPDFAVRPADVRGILARADDLGLRLVGDADGDVLDRMRDARAEA
                     TGDDAPVYAMLRLTFRRR"
     gene            3082..4038
                     /locus_tag="PL014_04620"
     CDS             3082..4038
                     /locus_tag="PL014_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOT56406.1"
                     /translation="MKTEVAGGRRRTPLDTLRWVALAVVLAAAGVALWRNWAQVSGEL
                     GQMGWATVALAFVLVVAAPVLTLMGWRVLLADLGTHLPAAPASSVFLVGQLGKYLPGS
                     VWSVVAQAEMGARLHVPRRRMAVVGILSIGLAVLTGCLLGVPALPRLVDRDADAFSWW
                     WIALAVGLGAVLLWPRLLNAAITRGLRLLRREPLEHELSGRAIALTSAWFVGAWVSTG
                     LGTFVLARSVAPEVPLGPLLVTSVCGFALASAAGMFSILVPAGVGVRDGVLALLLLTL
                     MPLPAATAVVVVARFLAVLADLVLAGAAWAWGRRHHLLGSSS"
     gene            4035..4844
                     /locus_tag="PL014_04625"
     CDS             4035..4844
                     /locus_tag="PL014_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT56407.1"
                     /translation="MTDDQGREEQLAYSETMAKMLDEQARRQKAAKLIAVVRHALGVQ
                     RLDGLRALDVGCSAGFIADELALAGATTTGVDIDEPGLAKARARFGERVDFRVARGEA
                     LPFDDDTMDIVVLNHIYEHVVDPEAVVADIHRVLRPGGVLYLGIGHRWQVIEPHHRLP
                     FLSWLPRGAADRYMRLTGKGEHYYERYYTPAGLRRLFADFDVWDYTVPVLSDPSAFAA
                     GDNVPAWSRRVPPAVFRAALPLVPTYVWAAFKGASTPSGPALRVAPRHLGS"
     gene            4867..5799
                     /locus_tag="PL014_04630"
     CDS             4867..5799
                     /locus_tag="PL014_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOT56408.1"
                     /translation="MPVQESRSRVVRTSRPVPLGAVVSVFRRGGGDPTSRRDHDAWWF
                     GWLTPDGAVTLRLSVSAAAGEVAAQAWGAGSGWALEKVPDLVGERDDPTGFEPLHAPV
                     GEAWRVFPGWRVPRSGLVLQALVPSVIEQKVTGQEAFSGYRRLVTRFGEPAPGPGSDL
                     GLRVPPTAAGWAGIPSWEWLAAGVDPARSRTVVRLVRHAGRIEECADLPVEAARARLQ
                     ALPGVGRWTWAEVAQRALGLADEVSFGDYHVAKNIGWVLTGTPVDDEGLAELLQPYAG
                     HRYRVQRLLELAGANRPRRGPRMSPRTHLPVRGR"
     gene            6234..7010
                     /locus_tag="PL014_04635"
     CDS             6234..7010
                     /locus_tag="PL014_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56409.1"
                     /translation="MQTVVVGNGEPTGRALLWRAVWETGAGSVLDGVSALVASGLTMY
                     EPSVIDVSVPKANRSRQVEGVRRHRRRIMPASRRSGLPRVDVPLAAINGAAWAVSDRQ
                     AVLILCLVLQQRLTTPERLSAAWNECRTHMSPARQALLRITLGDLCNGAHSLGELDFA
                     GLCREYGLPEPSRQVVRALPGGRVYLDAAWEDVGLVVEVDGGHHALALHVVDDALRQN
                     SVVLREERVLRVPVLGLRLVPDQFMAQVVEAHRLLSAAAA"
     gene            complement(7146..8285)
                     /locus_tag="PL014_04640"
     CDS             complement(7146..8285)
                     /locus_tag="PL014_04640"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOT56410.1"
                     /translation="MSGPRMPVRLDASGSPVTSVTVLGVRGIGEVVAGADVASLVVDA
                     LAHDGVPLGAGDCLVISSKVASKALGLTWSGSKEDAVAAGTVRVVAERWAEGRPTRVV
                     ESVAGPVMAAAGVDASNTGPSAALLVLPDDPDAVAARLRSDVLALLGLPEATPFAVVL
                     SDTAGRAWRGGLTDFALGSAGLLVLEDLRGGVDHDGRPLAVTMRAVADEVAASADLVK
                     GKANGIPAALVRGLDPACFDASADGARRLVRTGPGDWFALGHVEAVRAALGAAPGSDE
                     ALEVGVASAGGRDDVAARVGRVVALALLEVPDGSADVEVAGDAAEVTAGATDDYELGR
                     LVTRLEVAAHSEALRATVTARGPLCVTLTLSEPDPAVTSHASRQS"
     gene            complement(8282..9259)
                     /gene="cofD"
                     /locus_tag="PL014_04645"
     CDS             complement(8282..9259)
                     /gene="cofD"
                     /locus_tag="PL014_04645"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HOT56411.1"
                     /translation="MHITALAGGVGGARFIRGLRHHLDTTPGLADSTVSVIANTGDDI
                     TLFGLRVSPDVDTLLYTLGNGVHEGQGWGRADESHRVQGELAAYGALPQWFALGDLDF
                     GTHIVRSQWLGQGVPLSEVTARLAARWGLPERRITLLPMSDVPVETHVVVADGEDGAE
                     RAIHFQEWWVRHQASIPAQRFVVAGLDRATAAPGVLDAIRSADVVLLPPSNPVVSIGI
                     ILGVPGVRDALRGTTAPVVGISPLISGRPIRGHADACLSAIGVDSTAQAVAGLYEDFL
                     DGWLVDPADASTRARARVEAFDGPLLMTDVDATARIAAHALRLADAVRA"
     gene            9443..9718
                     /locus_tag="PL014_04650"
     CDS             9443..9718
                     /locus_tag="PL014_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT56412.1"
                     /translation="MNELYLIDGMLEESDADDGGLSWQERSLCAQTDPEAFFPEKGGS
                     TREAKKVCVGCDVRSECLEYALENDERFGIWGGLSERERRKLKKRAV"
     gene            9774..12887
                     /locus_tag="PL014_04655"
     CDS             9774..12887
                     /locus_tag="PL014_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56413.1"
                     /translation="MTLAPARPTTSPSDPLTPDGAPATVAAVVITRRDPGGLAELLTA
                     VLEQSLLPDAVLVLDRTPPGSPAAGTVSEVVDQARSSHRVPITIAPGDQRSPVRTVIH
                     RALLDQADPTTLAWLLPVGITPETGALVALVDDWRRSPSTGILGPKHVDAEHPNLLQG
                     LSIRTTRGGRLLPRPAPGEPDQGQYDRLSDVLAVPLPGSLVERDLLADLGGWERSFGD
                     VGADLDLGWRAHAAGRRVVVVPSARMHTEADLALVSAPTPSQRRAARRVALARAPWWT
                     VPAMALWIAVTSVLAALGLLLLKRPRAAWAEFSSLASLDPFRGMLARWRTRRRREVGR
                     RDLRTLFEPRRAVLTSWADAVHDAIVGPRQPVGEEAHEHTPRSWLSTALRTPGVLAGV
                     GAAIVAAGAGRSLGVGVLTGAGSGLVGGELVGTRADAATLWHSWVDGWSGAGLGGPDP
                     VAPSAALLALPTRVVDALPLVPQASSPAGLVVALLVIVGMPLAAVSAYLALRPVAGTR
                     WVRALAAFAWATTGVAAASVAQGRVGALVGLVLLPPLACGLWLIAARRSTATSAFATA
                     LAAVLLGAFAPVLLAPVVVLALVLFLVRSRVRLHALVVAVVPVAVLAPWLVRAAETSW
                     PALVAGSGLAQWGGQAPLPWQIALLSPGGAGTPLVWTAAPLVALAALSLVRGRSWGGS
                     GAVLAVLAPVLLALALIAPSVQLGTIPAGLEGAGEPITVWAGTMLLPFALVVVVALAR
                     GLDSMALGRSTSTRLAPVLARRAVASAGILAVLVSAGGVVWATLGSELAPWVDPRPAV
                     SIEQAEGAFATRALFVSPGERGAGYRFVGREAADVVRPLPVVTEADAAVASAVTSMIG
                     DASSGAELFSGTATDLLAVRGDVDLEVTRRLDATEGLQRIAPREGWQMWRVSPRTGEP
                     DERSLVAPPRVRLVSPEGVVLVPTTGLHAATEASLVAPSGGQLVVAAPAAWAEHAVVA
                     VDGAVLDPVDGAPTPTYEVPVGGSRLAITLTNPDRWWQVGQVVAVFVLAFLAVPFGRR
                     ESRVRAG"
     gene            12884..14455
                     /locus_tag="PL014_04660"
     CDS             12884..14455
                     /locus_tag="PL014_04660"
                     /inference="COORDINATES: protein motif:HMM:NF040160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HOT56414.1"
                     /translation="MNAVSRGLSRAGKALRAMNWAGITRVVVPAGVAVGLVAFTTANP
                     REFDLVQASGATESSVAGVSLATRVTQACPGPELSGIPGVPDATIPATVTAAAGPEEL
                     LPAPVTGDGRLVAASGGTEVLSLGTRPGVQSAPLGGAGAVVLTGEGPLAPAVAGTQEW
                     RVERPELRGLVSVPCGPGAAEHWLLGGGEGPGRQERLVLSNPGANPVTADVTVHGAAG
                     PLADPYVETVPPGGRVSLLLDAKAGEESLLAVHVRAAGGGVHATLTDTWIEGSRALGA
                     ETTSATAAPGEVQVVPGVAFGGGPTAVRVAVPGDQDAVVRVSVFGPEGLVPTTGESVL
                     SVVAGGVGQLEVRGVPAGTYAVALRADVPVVASVLSRTVASSAASTQAEGTSGDQATP
                     GDFAWATSVEGVASAVPDDQTAPVAAAAFDPTGEVDRTLHLISVGGNGTVEVLTVVDG
                     TARSRRIDLLGDRLVMVDLSGASSVWVRPVTGSGLVHGSVLSGVGEGESRLISSMPLQ
                     ASVVTSAVSRAFPLP"
     gene            complement(14459..14803)
                     /locus_tag="PL014_04665"
     CDS             complement(14459..14803)
                     /locus_tag="PL014_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOT56415.1"
                     /translation="MRSRAEHFDALVLDCADRVRPHLGTRYAALEFAVEEVPPQDPAP
                     WEEQTPPLGRLLRGTPDRDPRVVVYRRPIEARAHDDADLAEIVREVVTEQVAALLGVP
                     PHEIDPDRDPDA"
     gene            14974..15408
                     /locus_tag="PL014_04670"
     CDS             14974..15408
                     /locus_tag="PL014_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HOT56416.1"
                     /translation="MNVSRRCSKTACRQPAVATLTYVYADQAAVLGPLATYAEPHTYD
                     LCHQHATRLTAPRGWEVVRLVAEYPEPQPSTDDLLALADAVREAGRPRRSQPPAAPTA
                     APAAASVAAAGTASEGYDRGPTVVTGEVRRGHLRMLPSATDS"
     gene            15506..16939
                     /locus_tag="PL014_04675"
     CDS             15506..16939
                     /locus_tag="PL014_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOT56417.1"
                     /translation="MTATSLAHVVKAYDVRGTVPDQLDTRVTRALGAAFADVVVIPEG
                     AGACVVGHDMRDTSPELSSAFADGARSRGVDVTLIGLASTDGLYYASGALDLPGAMFT
                     ASHNPAAYNGIKLCRAGARPVGQDSGLSEIRVLAERILDGEVPDPDVTPGELDERDVL
                     ADYASFLRGLVDLSGIRPLRVVVDAGNGMGGFTVPAVLEAGAGLPALPLTIVPLYFEL
                     DGTFPNHEANPLDPKNLVDLQAAVVEHGADLGLAFDGDADRCFVVDERGEPVSPSAIT
                     ALIARREVARAVAQGATASDVAIVHNVICSAQVPEVIAETGARAVRTRVGHSFIKAEM
                     ARHDAVFGGEHSAHYYFRDFWFADTGMLAAMHTLAALGEQDAPLSVVMGDLHRYAASG
                     EINSTVSDAAAATVRVRAWAEGEGVVADELDGLTLTHAGDGAGDPMWWLNLRASNTEP
                     LLRLNVEAADVATMERVRDTALALIRA"
     gene            16990..>17187
                     /locus_tag="PL014_04680"
     CDS             16990..>17187
                     /locus_tag="PL014_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56418.1"
                     /translation="MSEQATPASAIEPWLREILRCPACRSELRDDTGPTGGPELVCTS
                     GECGLAYRIDDGVPVLLVDEAR"
BASE COUNT         2063 a         6356 c         6316 g         2452 t
ORIGIN      
        1 caccgtcccc acatccgcgg gggccgccgg ccgcaccgtc cccacgcctg ctggagccga
       61 cgaccacgca cacctgcacg ctgcacccac cgagaagatc tcggtgcgct ccggtgagat
      121 ggccgggcgc agcaacgacc cggccggccc ggcatccccc aacgccgccg gggaggcggc
      181 gtcacccgtg gcgcgcaccg gccagtcgag ctacccgatc gaggacggcc gcgacggccc
      241 cggccccgcg ccgtacgtca cgatcgtgct gccctgctac aacgagggcg cgcacgtgct
      301 gaaggagatc gaccgcatca ccgcggcgat gaccgccagc gagctcacct acgagttgct
      361 ctgcatcgac gacgccagca ccgacgacac cctcgacgtg ctgcgccacg cccagcagaa
      421 ctacccgaac ctgcggatcc tgccgttccg tcgcaacggc ggctcgggca ccgcccggcg
      481 catcggcacc cagcaggccc gcggcgagat cgtcgtgtgg accgacgccg acatgaccta
      541 cgagaacgag cgcatccccg agctcgtgcg gatccttcgc gacgacgcca gctacgacca
      601 ggtggtcggc gcccgcacca ccgaggaggg cacccacaag tgggcgcgcg tgcccgccaa
      661 gtggctcatc cgcaacatcg cccagtggct caccaagacc aagatccccg acctgaactc
      721 gggcctgcgg gccttccgtc gcgaggtgtc gctgccgtac ctgcgcctgc tgcccgccgg
      781 gttctcctgc gtcacgacga tcaccatcgc gttcctggcc aaccagcacg acatcaagta
      841 cgtcgagacc agctacgcca agcgcgccgg ggtgagcaag ttccacttcg tcggcgacgc
      901 ctaccgctac atcctccagg tgctgcgcat ggtcatgtac ttcgacccgc tcaaggtgct
      961 catgcccccg gccctgtgga tgatcgtcct cggcgtcggc aaggccgtcg tcgacatggt
     1021 gcgccacccc ttctacttcc cggcgagcac ggtgctgctc atcgtcagcg gcatcatgat
     1081 cgccagcctg gccctgctct ccgacctcgt ggtgagatcg cgagacggtg tctgagcgcg
     1141 acgcgtcgtc gggcagcgcc gcgctcaccg tcgcgctcgt cggccccacg caccccttca
     1201 agggtggggt ggcggcccac acgacgaccc tcgcgcacga gctgagcgag gccggccacg
     1261 aggtcaccct cgtgtcgtgg agccacctct acccgagcgc gctctatccc ggcgagcagt
     1321 ccgtgcccgg cggagccccc gacgtcgagc cgttcccgcg caccctgcgc gtgctctcct
     1381 gggcgcgccc ggacacctgg ctgcgcaccg gtcgccgcct gcgtgacgtc gacgtcatcg
     1441 tcgtcgtgca cgtcatcccc gccgtggtcc ccgcccacct ggccctgctg cgcgccgctg
     1501 gcgccggccg ccgcacggca tccgggcacg ggccgcgcac gatcctcatc gcccacaacg
     1561 tgctgccgca cgagagccac cccggggcgc gccagctcgt cgagtcgctc gtggagcggg
     1621 tggatgccgt cgtggtccac tccgacgagc aggcccgcct ggcgacgacc ctcggcgccc
     1681 accacgtgcg ccggctcgac ctcccaccgc accttcccgg gggagacccc gagccccgcg
     1741 tggcgaacga cgctgacaca gccagcactc ccgtgcgggt gctcgccctc ggcatcgtgc
     1801 gcgactacaa gggggtcgac ctcctcgtcg acgcggtgcg cgacgtgccc ggcatccacc
     1861 tcaccatcgc gggggagttg tggggggatg ccggtcgcac cgttcgcgac ctcgcgaacg
     1921 acccgcgcgt cgcgggtcgc gtcgagctgc acccggggta cgtgcccgcc gatcggatcg
     1981 ccgggctcct ggcccagcac gacgtcatgg ccctgaccta ccggacggcc accgcctcgc
     2041 agaacgtgct gctcgcccac aagcacggcc tgcccgtgct cgcaaccacc gtcggcacct
     2101 tccccgagca gatccgcgac ggcgtcgacg ggctgctcgt cccgccgggt gaccgggggg
     2161 ccctcgtcga ggcgctgcgc cgtctcgctg agcccggcgt caccgaggcg ttgcgctcgg
     2221 cagtgcgccc gcccgacctc tccgggccgt gggcgaacta cgtcgggggc atcgaggcgc
     2281 tcgccgcggc cccgctcggc cccgtcgacg aagccgactc cgagggcggc ggggccgggc
     2341 tccggctcgg cccggtggcg cgcgcgaagg cgaccgtcgc cggcatccgg gctcgccgtc
     2401 gccccgtcgt cgagatcgcc ccgggtgaca tcccgtcgcg ggtgcgagcg accgacgtgc
     2461 tggccctgga ctccgacgcc gtcgatgccg tcgccatcgc ccgggagctg ggcctgcccc
     2521 ggcccggcga gccggttgcg gcctgggcgg ccctcggggc gctcgccgcg gtggtgcgca
     2581 tccgggacga cggccaccgc tcctcggtga tcgtcgacga gtccggcagc ggcagcccgc
     2641 tcggccgctg ggcgagggcc gtcggcttcg cccccgtcga gctcgagctg accgggcaac
     2701 gcgctgccgt cgccgtgctc gacatcgaca ccgcgagcct ggacgtcatc acccggctgc
     2761 acccgggcgg ctgcacggca tccgacgtcg acgtcgccat cggtcaggcg tcgtgggcgc
     2821 tgcgctcggg tggcctgctc atcctcacgc tgcccatcgg cgacgacgac cccgacttcg
     2881 cggtgcgccc ggccgacgtg cgcggcatcc tcgcgcgcgc cgacgacctc gggctgcgcc
     2941 ttgtcggtga cgccgacggt gacgtgctcg accgcatgcg tgacgcccgc gccgaggcca
     3001 cgggtgacga cgccccggtc tacgcgatgc tccgcctgac cttccggcgg cgctgacatg
     3061 accgggcggg gcgacgaggt ggtgaagacg gaggtggccg gggggcgtcg gcgcaccccg
     3121 ctcgacaccc tgcgctgggt ggcgctcgcg gtggtgctcg cggcggccgg ggtggcgctc
     3181 tggcgcaact gggcccaggt gtccggcgag ctcggccaga tggggtgggc caccgtggcg
     3241 ctggccttcg tgctcgtcgt cgcggcgccg gtgctcaccc tcatggggtg gcgcgtgctg
     3301 ctcgcggacc tcggcaccca cctcccggcc gcaccggcgt ccagcgtctt cctcgtgggt
     3361 cagctcggca agtacctgcc ggggtcggtg tggtccgtcg tggcccaggc cgagatgggg
     3421 gcccggctgc acgtgccacg gcggcggatg gccgtcgtcg gcatcctgtc gatcgggttg
     3481 gccgtgctca ccgggtgcct gctcggagtg cccgccctgc cgcggctcgt cgaccgtgac
     3541 gccgatgcct tctcgtggtg gtggatcgca ctcgccgtgg gtctcggggc ggtgctgctg
     3601 tggccgcggc tgctcaacgc cgccatcacc cgggggctgc gactgctgcg gcgagaaccc
     3661 ctcgagcacg agctgtcggg gcgcgcgatc gccctgacct cggcctggtt cgtcggggcg
     3721 tgggtctcca ccgggctcgg gaccttcgtg ctcgcgcgca gcgtcgcccc cgaggtgccc
     3781 ctcggcccgc tgctcgtcac gagcgtgtgc ggcttcgcgc tcgcgtccgc agccggcatg
     3841 ttcagcatcc tcgtgccggc gggcgtcggg gtgcgcgacg gcgtgctcgc cctcctgctc
     3901 ctcacgctca tgccgctgcc cgcggccacc gcggtggtcg tcgtcgcccg gttcctcgcc
     3961 gtgctcgcgg acctggtgct cgcgggtgcc gcgtgggcct gggggcgtcg ccaccacctc
     4021 ctaggatcga gctcgtgacc gacgaccagg gccgtgagga gcagctggcc tactccgaga
     4081 cgatggccaa gatgctcgac gagcaggccc ggcgtcagaa ggcggccaag ctcatcgcgg
     4141 tggtgcgcca cgccctcggg gtccagcggc tcgacgggct gcgcgccctc gacgtcggct
     4201 gctcggcagg gttcatcgcc gacgagctcg ccctcgccgg ggcgacgacg accggcgtcg
     4261 acatcgacga gcccggcctg gcgaaggccc gggcccgctt cggcgagcgg gtcgacttcc
     4321 gggtggcccg cggcgaggcg ctgcccttcg acgacgacac catggacatc gtcgtgctca
     4381 accacatcta cgagcacgtg gtcgaccccg aagccgtcgt cgccgacatc caccgggtgc
     4441 tgcgcccggg cggggtgctc tacctcggca tcggccaccg ctggcaggtg atcgagccgc
     4501 accaccggct gccgttcctg tcctggctgc cgcgtggtgc cgcggaccgc tacatgcggc
     4561 tcaccggcaa gggcgagcac tactacgagc gctactacac cccggccggg ttgcgccgcc
     4621 tcttcgctga cttcgacgtg tgggactaca ccgtgccggt gttgtccgac cccagcgcct
     4681 ttgccgccgg cgacaacgtg cccgcgtggt cccgccgggt gccgccggcc gtgttccggg
     4741 cggcgctccc gctcgtgccg acctacgtgt gggctgcctt caagggtgcc tcgaccccga
     4801 gcgggcctgc cctgcgggtg gctccccgtc acctcggctc ctgagtcaag gaggcgagcc
     4861 gctcggatgc cggtgcagga gagtcgttcg cgggtggttc gcacctcccg cccggtgccg
     4921 ctcggggcgg tggtctcggt gttccgccgg ggtggcggag accccacgag tcggcgcgac
     4981 catgacgcct ggtggttcgg ctggctcacg cccgacggcg cggtgacgct caggctgtcg
     5041 gtctccgctg ctgcgggcga ggtggcggcg caggcctggg gggccggctc ggggtgggcc
     5101 ctcgaaaagg tgcccgacct cgtgggtgag cgtgacgacc ccacggggtt cgagccgctc
     5161 catgccccgg tgggggaggc gtggcgggtc tttccagggt ggcgggtgcc gcgctcgggc
     5221 ctggtgctcc aggcgctggt gccgtcggtg atcgagcaga aggtgaccgg gcaggaggcc
     5281 ttctcggggt accggcgact ggtgacccgc ttcggggagc ctgcccccgg gcctggatcc
     5341 gacctcggtc tgcgggtacc accgacggcg gcggggtggg ccggcatccc ctcgtgggag
     5401 tggttggccg ccggggttga tccggcgcgc tcgcgcaccg tggtgcggtt ggttcgtcat
     5461 gcggggcgga tcgaggagtg tgccgacctg ccggtggagg ctgcccgtgc tcggctgcag
     5521 gcgctgccgg gcgtgggccg ctggacgtgg gccgaggtgg cgcagcgggc gctggggctg
     5581 gccgacgagg tgagcttcgg cgactaccac gtggccaaga acatcggctg ggtgctgacc
     5641 ggcacgcccg tcgacgacga ggggctggcg gagttgcttc agccgtatgc cgggcaccgc
     5701 taccgcgtgc agcggctgct cgagctggcg ggagcgaacc ggcctcgacg tggcccgcgg
     5761 atgtcgccgc gcacccatct gccggtgcgc ggtcgctgat cggacctggt gtcgggtggc
     5821 tcgcggaggg ggcgcctggt ggggtggggt gtcaggggcg cggctggttg cgcggttggc
     5881 atcccttggg gtggggatgc cggcggagtg gggtgtcagg ggcgcggttg gttgcgcggt
     5941 tggcacccca tgagcagctc tgagcaagct catcggcacc cgcgacctgt ggatatcgcc
     6001 aatcagtcgt ggatccgcgc gagagttggc cgatggagag cgcgcaacct caggctggca
     6061 ccgccccaag gccccgacgg tcgagactgg ctcgaccgga tcgggcccga ctggccgagt
     6121 cggtcgcagg tgaccacgac ggggttgccc atcgatccga cctgcgatcc gtcggcatca
     6181 cccgtgccga tgtgcgaagt gaggtctcgg ccggccgatg gctcacgagg ggcgtgcaga
     6241 ccgtcgtcgt cggcaatggg gagcccacgg gcagagccct gctctggcgc gcagtctggg
     6301 agaccggtgc tgggtccgtc ctcgacggag tgtcagcgct ggtcgcctcc ggcctcacca
     6361 tgtacgagcc gtcggtcatc gacgtctccg tgccgaaggc gaatcggagc aggcaggtcg
     6421 agggagtgcg acgacaccgg cggcggatca tgcctgcctc ccgcaggtcg ggcttgccgc
     6481 gagttgacgt gccgttggcg gcgatcaacg gcgcggcgtg ggctgtgagc gaccggcagg
     6541 ccgtcctgat tctctgcctc gtgctgcagc agaggctcac cacgcctgaa cggttgtccg
     6601 cggcctggaa cgagtgcagg acccacatgt ctccggctcg ccaagcgctg ctgaggatca
     6661 cgcttggtga cctgtgcaac ggggcacact cgttgggtga gctcgacttc gctggcctct
     6721 gccgtgagta cggactcccc gagccgagcc gacaggtggt gagggctctc ccgggcggcc
     6781 gggtgtatct cgatgccgcg tgggaggacg tcggccttgt ggtggaggtc gatggagggc
     6841 accacgctct cgcgctccat gtcgtcgacg atgcgctgcg ccagaactcg gtcgtcctgc
     6901 gcgaagagcg cgtgctccgc gtccccgtgc tcgggctgcg gttggtacct gatcagttca
     6961 tggctcaggt cgttgaggcg caccgactcc tgagcgccgc ggctgcctga actcggccgg
     7021 cggggtgggg tgtcaggggc gcggctggtt gcgcggttgg catcccttgg tgctggagtc
     7081 gttgccggct gaaaggaatt ccttgcccgg ggtaacgacc ctgtacagca ccccctgagg
     7141 ctctctcagg attgccgcga agcgtgcgat gtgaccgctg gatcgggttc cgacagggtc
     7201 agcgtcacgc agagcggacc tctcgcagtg accgtggcgc gcagggcctc gctgtgggcc
     7261 gccacctcga ggcgggtcac gaggcggccc agctcgtagt cgtcggtggc gccagcggtg
     7321 acctcggccg cgtcgccggc gacctcgacg tcggccgacc cgtccgggac ctcgagcagg
     7381 gccagcgcga cgacgcgacc cacccgcgcg gccacgtcgt cgcggccacc ggccgatgcg
     7441 acgcccacct cgagggcctc gtcgctgccg ggcgcggcac ccagggcggc gcgcaccgcc
     7501 tcgacgtgtc ccagggcgaa ccagtccccg gggccggtgc gcacgagccg gcgagcgccg
     7561 tccgccgagg cgtcgaagca cgccgggtcc agtccgcgca cgagggcggc tgggatgccg
     7621 ttcgccttcc ccttcacgag gtctgcggac gcagcgacct cgtccgcgac cgcccgcatg
     7681 gtcaccgcga gcgggcgccc gtcgtggtcg acgccgccgc gaaggtcctc gagcacgagc
     7741 aggccggcgc tgcccagggc gaagtcggtg agccctcccc gccacgcacg gcccgcggtg
     7801 tcgctgagca cgaccgcgaa cggggtggcc tcgggcaggc cgagcagggc cagcacgtcc
     7861 gagcgcaacc gggctgcgac ggcatccgga tcatccggca gcaccagcag ggcggcggag
     7921 ggcccggtgt tcgacgcgtc gacgccggca gcggccatga ccggcccggc caccgactcg
     7981 acgacccggg tcggtcgacc ctcggcccac cgctcggcga cgacgcgcac ggtgccggcc
     8041 gccacggcat cctccttcga gccgctccag gtcaggccca gcgccttcga cgccaccttg
     8101 ctcgagatga cgaggcagtc gccggcaccg agggggaccc cgtcgtgcgc cagggcgtcg
     8161 acgacgaggc tggcgacatc ggccccggcc acgacctccc cgatgccacg aaccccgagc
     8221 accgtcaccg acgttaccgg tgaacccgac gcgtcgaggc gcaccggcat ccgcggacca
     8281 ctcacgcgcg caccgcgtcg gcgaggcgca gcgcgtgggc ggcgatgcgt gcggtcgcgt
     8341 cgacgtcggt catcagcagg ggcccgtcga acgcctcgac ccgggcgcgg gcgcgggtgg
     8401 aggcgtcggc gggatcgacg agccagccgt cgaggaagtc ctcgtacagg ccagcgaccg
     8461 cctgggccgt cgagtcgacg ccgatggcgc tcaggcaggc gtcggcgtgc ccgcggatcg
     8521 gccggcccga gatgaggggg gagatgccga cgacgggagc agtggtgccg cgcaacgcgt
     8581 cccgaacacc cggcacaccg aggatgatgc cgatcgacac caccgggttg ctcggcggca
     8641 gcagcacgac gtcggccgag cggatcgcgt cgagcacgcc cggggcggcg gtggcgcggt
     8701 cgaggccggc caccacgaac cgctgcgccg ggatcgacgc ctggtggcgc acccaccact
     8761 cctggaagtg gatcgcccgc tcagcaccat cctcaccatc ggccacgacc acgtgggtct
     8821 cgacggggac gtcggacatc ggcagcagcg tgatccgtcg ttcgggcagt ccccagcggg
     8881 ccgccaggcg cgcggtcacc tcgctgagcg gcaccccctg acccagccac tgggagcgca
     8941 cgatgtgcgt gccgaagtcg aggtcaccga gggcgaacca ctgcggcagc gcgccgtagg
     9001 ccgcgagctc gccctgcacg cggtggctct cgtcggcgcg gccccatccc tggccctcgt
     9061 ggacgccgtt gccgagggtg tagagcaggg tgtcgacgtc ggggctcacc cgcaggccga
     9121 acaaggtgat gtcgtcaccg gtgttggcga tgacgctcac cgtggagtcg gccaggccgg
     9181 gggtggtgtc gaggtggtga cgcagtccgc ggatgaaccg ggcgccgccg accccgcctg
     9241 cgagagcggt gatgtgcatg gcgccagtgt cccagcaggg ccgttgagtc ggcgattcgc
     9301 ccacgaaacg ccgcggatca cgcaaaaagg ggagcaataa caccaaccgg cttgacgcag
     9361 ggtgcatgac acgcgtgtaa ttccatgcat gtgctccggg tgcccgaccg gttcacatgg
     9421 gtcgaggggc gaggagatga cgatgaacga gctgtacctg atcgacggaa tgctcgagga
     9481 gagcgacgcc gacgacggcg ggctgtcctg gcaggagcgc tcgctgtgcg cccagaccga
     9541 ccccgaggcg ttcttccccg aaaagggtgg ctccacccgc gaggccaaga aggtctgcgt
     9601 cgggtgcgac gtgcgctccg agtgcctgga gtacgcgctg gagaacgacg agcgcttcgg
     9661 catctggggc ggcctgtccg agcgcgagcg ccgcaagctc aagaagcgcg cggtgtgacg
     9721 agcggccccc gcaggccgtc ctcacgccta ctcggaccgt cccgccagac cgcatgacct
     9781 tggcccccgc gcgccccacg acaagcccgt ccgaccccct caccccggac ggtgccccag
     9841 cgacggtggc cgcagtggtc atcacccgcc gcgaccccgg cgggctggcc gagctcctca
     9901 cggcagttct cgagcagtcg ctcctgccgg atgccgtgct cgtccttgac cgcacgcccc
     9961 ccgggtcgcc cgcggccggg accgtcagcg aggtcgtcga ccaggcccgc agcagccacc
    10021 gcgtgcccat cacgatcgcc cccggcgacc aacggtcccc ggtacgcacc gtcatccacc
    10081 gagccctgct cgaccaggcc gaccccacga ccctggcctg gctcctgccc gtcggcatca
    10141 ctcccgagac cggcgccctc gtcgccctcg tcgatgactg gcggcgttcg ccgtccaccg
    10201 gcatcctcgg cccgaagcac gtcgacgccg agcacccgaa cctccttcag ggcctgtcga
    10261 tccgcaccac ccgcggtggt cggctcctgc cgcgacctgc gccgggtgag cccgatcagg
    10321 gtcagtacga ccggctgagc gacgtcctcg ccgtgccgct gcccggctcc ctcgtcgagc
    10381 gtgacctgct cgccgacctc ggcgggtggg agcgcagctt cggcgacgtc ggggccgacc
    10441 tcgacctcgg gtggcgagcg cacgcggccg gacgccgcgt cgtcgtcgtg ccctccgcgc
    10501 gcatgcacac cgaggccgat ctggccctgg tgtcggcgcc cacccccagc cagcgccggg
    10561 ctgcccgccg agtggctctc gcccgggcgc cgtggtggac ggtgcctgcc atggcgttgt
    10621 ggatcgccgt caccagcgtg ctcgccgccc tggggctgtt gctgctcaag cggccccgtg
    10681 cggcgtgggc cgagttctcg tcgctggcct ccctcgaccc gttccgggga atgctcgccc
    10741 gctggcgcac gcgtcggcgc cgcgaggtgg gccgccgcga cctgcggaca ctgttcgaac
    10801 cgcgtcgcgc cgtgctcacg tcctgggcgg atgccgtgca cgacgccatc gtcgggccgc
    10861 gccagcccgt gggtgaagag gcccacgagc acaccccccg gtcatggctg tccaccgcgc
    10921 tgcgcacccc cggggtgctc gcgggcgtcg gcgcggccat cgtcgcggcg ggtgcagggc
    10981 gctctctcgg ggtcggggtg ctgaccggtg ccgggtcagg gctcgtcggc ggtgagctgg
    11041 tcggcacccg tgccgacgcc gccacgttgt ggcactcctg ggtcgacggc tggtcgggag
    11101 ccggcctcgg cggccccgac ccggtcgccc cgtcggcggc cctgctcgcg ctgcccaccc
    11161 gggtcgtcga cgcgctgcca ctggtgccgc aggcgtcgtc gccggccggg ttggtcgtcg
    11221 cactcctcgt catcgtgggg atgccgctcg cggccgtgag cgcctacctc gccctacggc
    11281 cggtggccgg cacccggtgg gtgcgggccc ttgctgcgtt tgcgtgggcc accacgggtg
    11341 tcgccgcggc gtcggtggcc caggggaggg tgggggccct cgtcgggctg gtcctgctgc
    11401 cgccgctggc ctgcgggttg tggctcatcg cggcccgccg gtcgacggcg acgagtgcct
    11461 tcgcgacggc gctggctgcc gtgcttctgg gggccttcgc gccggtgctg ctcgcccccg
    11521 tcgtggtgct cgccctcgtt ctgttcctcg tgcgcagccg ggtgcgcctg cacgcgctgg
    11581 tcgtcgccgt ggtcccggtc gcggtgctgg caccgtggct cgtgcgggcc gctgagacgt
    11641 cctggcccgc actcgtcgcc ggttccgggc tggcgcagtg ggggggtcag gcgcccctgc
    11701 cgtggcagat tgccctgctc agccccggcg gcgcagggac tccgctcgtg tggaccgcgg
    11761 cgccgctcgt ggcgctcgct gccctgtcgt tggtgcgggg ccgctcgtgg ggtggctcgg
    11821 gggccgtgct cgccgtgctc gcccccgtgc tgttggcgct cgcgctgatc gccccgtccg
    11881 tgcagctcgg cacgatcccc gccgggctgg agggcgcggg cgaaccgatc accgtgtggg
    11941 ccggcaccat gctcctgccg ttcgcgctcg tcgtcgtcgt cgccctggcc cggggcctcg
    12001 acagcatggc gctgggccgg tcgacgtcga cccgactcgc ccccgtcctg gcgcggcgcg
    12061 ccgtggcgag cgccggcatc ctcgccgtcc tcgtgtccgc cgggggagtg gtctgggcga
    12121 cgctcggcag cgagctcgcg ccgtgggtcg acccgcggcc agccgtatcc atcgagcagg
    12181 ccgagggcgc cttcgcgacc cgggcgctgt tcgtcagccc gggggagcgc ggtgcgggct
    12241 accggttcgt cggccgcgag gctgccgacg tcgtgcgccc gctgcccgtc gtgaccgagg
    12301 cggatgcggc ggtcgcctcc gcggtcacgt cgatgatcgg tgacgcgtcg tcgggggcag
    12361 agctgttctc cggcaccgcc accgacctgc tcgccgtgcg cggcgacgtc gacctcgagg
    12421 tcacccgccg gctcgacgcc accgaggggt tgcagcggat cgccccgcgc gaggggtggc
    12481 agatgtggcg ggtcagcccg cgcaccgggg agcccgacga acgctcgctc gtggccccgc
    12541 cacgggtgcg gctggtgagc cccgagggcg tcgtgctcgt gccgacgacg gggctgcacg
    12601 ccgccaccga ggccagcctc gtggccccct cgggcggaca gctcgtcgtg gccgcgcccg
    12661 ccgcctgggc cgagcacgcc gtcgtcgcgg tggacggggc cgtcctcgac cccgtggacg
    12721 gtgcaccgac cccgacctac gaggttccgg tcggcggcag tcgactggcc atcaccctga
    12781 ccaaccccga ccgctggtgg caggtcggcc aggtcgtggc ggtgttcgtc ctcgccttcc
    12841 tggccgtgcc cttcgggcgt cgcgagtcac gggtgcgtgc cggatgaacg cggtgtcacg
    12901 gggtctgtcc cgtgccggta aggcgctgcg ggcaatgaac tgggcgggga tcacgcgggt
    12961 cgtcgtcccg gccggggtcg ctgtcgggct cgtggcgttc acgaccgcga acccccgcga
    13021 gttcgatctg gtgcaggcct ccggtgccac tgagtcgtcg gtggccgggg tctccctggc
    13081 gacccgggtc acccaggcct gtccgggccc cgagctcagc ggcatccccg gcgttccgga
    13141 cgccacgatc ccggcgaccg tgaccgcggc ggccgggccc gaggagctgc tgcccgcccc
    13201 ggtgaccggc gacgggcggc tcgtcgcggc atccggaggc actgaggtgc tctcgctcgg
    13261 cacccgccca ggggtgcaga gcgccccgct cggaggggcc ggggccgtgg tgctgaccgg
    13321 tgaggggccg cttgctcccg ccgtcgccgg aacccaggaa tggcgggtcg agcgccctga
    13381 gctgcggggt ctggtcagcg tgccgtgcgg gcccggtgcc gcagagcact ggctgctcgg
    13441 cggcggcgag ggcccgggtc gccaggagcg gctcgtgctc tcgaaccccg gcgccaaccc
    13501 ggtgaccgcc gacgtcaccg tgcacggcgc cgccgggccg ctcgccgacc cgtacgtcga
    13561 gaccgtgccc cccggtggcc gggtcagcct gctgctcgac gccaaggctg gtgaggagtc
    13621 actgctcgcc gtgcacgtgc gcgccgccgg tggtggggtg cacgccacgc tgaccgacac
    13681 gtggatcgag ggcagccggg cgctgggtgc cgagaccacc tccgcgaccg cggcacccgg
    13741 tgaggtccag gtcgtgccgg gggtggcgtt cggtggcggg ccgaccgccg tgcgggtcgc
    13801 ggtgcccggt gaccaggatg ccgtggtccg ggtcagcgtg ttcggcccgg agggcctggt
    13861 cccgacgacg ggggagagcg tgctgagcgt cgtcgccgga ggggtcgggc agctcgaggt
    13921 gcgaggggtt cccgccggga cctatgcggt ggcgctgcgg gccgacgtgc ccgtggtggc
    13981 gtcggtgctc agccggacgg tcgcgtcatc ggccgcgagc acccaggctg agggcacgtc
    14041 gggcgaccag gccacaccgg gagacttcgc gtgggcgacg tccgtcgagg gggtcgcgtc
    14101 agcggtgccg gacgaccaga cggcgccggt ggcagcagca gcgttcgacc ccactggcga
    14161 ggtggaccgc accctgcacc tcatctccgt cggcgggaac ggcacggtcg aggtgctgac
    14221 ggtcgtcgac ggcacggcgc gctcgcgcag gatcgacctg ctcggcgacc gcctcgtgat
    14281 ggtcgacctc agcggcgcga gttcggtgtg ggtgcgcccg gtgaccggca gcggcctcgt
    14341 gcacggctcg gtgctcagcg gggtcggtga gggtgagtcg cggttgatct cgtcgatgcc
    14401 gctgcaggcc agcgtcgtca cctcggcggt cagccgcgcg ttccccctgc cctgacgctc
    14461 aggcgtcggg gtcgcggtcc gggtcgatct cgtggggcgg cacgccgagc agcgcggcca
    14521 cctgctcggt gaccacctcc cgcacgatct cggcgaggtc ggcgtcgtcg tgtgcacggg
    14581 cctcgatcgg cctgcggtag acgacgaccc gcgggtcgcg atcgggggtg ccccgcagca
    14641 gccgccccag cggtggggtc tgctcctccc acggggccgg gtcctgcggc ggcacctcct
    14701 cgacggcgaa ctcgagcgcg gcataccgcg tgccgaggtg ggggcgcaca cggtcggcgc
    14761 agtcgagcac gagcgcgtcg aagtgctctg cgcggctgcg catcgccggc accggcggcc
    14821 acgccatcgg cccccggatc cctcgcccgt gccggtccct gcgtcctgat gggctcacgg
    14881 ttggcatggt acggccagcg tgcaggcagt cgtccgaccc gccagcggcg tgcctgcgcc
    14941 gggtcgtcgg tgggtcggcc tagagtcgac gacgtgaatg tgtctcgtcg gtgttccaag
    15001 accgcgtgcc gccaaccggc ggtcgccacc ttgacgtacg tctacgccga ccaggctgcc
    15061 gtcctcggtc cgctcgcgac ctacgccgag ccgcacacct acgacctgtg ccaccagcac
    15121 gcgacccgcc tgaccgcgcc gcgaggctgg gaggtcgtgc gcctcgtcgc cgagtacccc
    15181 gaaccgcagc cgagcaccga tgacctcctc gccctcgcgg atgccgtgcg cgaggccggt
    15241 cgtccgcggc ggtcccagcc cccggcggcc ccgaccgctg cgccagcggc ggcgtccgtc
    15301 gctgcggcgg gtacggcatc cgagggctac gaccgcggcc cgaccgtggt caccggcgag
    15361 gtccgccggg gccacctgcg gatgctgccc agcgcaaccg acagctgagc accccacctc
    15421 gcgccacgag tgcgcacgtg gttgccaggg cagccacgtc cgcactcacg gcggattcgg
    15481 atgccgtcgc gtaggctcgc ctgccgtgac tgcaacctcg ctcgcccacg tcgtcaaggc
    15541 ctacgacgtc cgcggaacgg tccctgacca gctcgacacc cgggtcacgc gcgccctcgg
    15601 cgccgcgttc gccgatgtcg tcgtcatccc cgagggggcc ggggcgtgcg tcgtcggcca
    15661 cgacatgcgc gacacgagcc ccgagctgtc gtccgcgttc gccgacgggg cgcgcagccg
    15721 cggggtcgac gtcaccctca tcggcctcgc gagcaccgac ggcctctact acgcgagcgg
    15781 cgcgctcgac ctgcccggcg cgatgttcac cgccagccac aaccccgccg cgtacaacgg
    15841 catcaagctc tgccgcgccg gggcccggcc cgtcggccag gacagcgggc tctccgagat
    15901 ccgcgtgctc gccgagcgca tcctcgacgg tgaggtgccc gaccccgacg tgaccccggg
    15961 cgagctcgac gagcgtgatg tcctcgccga ctacgcgtcg ttcctgcgcg ggctcgtcga
    16021 cctgtccggc atccggccgc tgcgggtcgt cgtcgacgcc gggaacggga tgggcgggtt
    16081 caccgtgcca gcggtcctcg aggcgggtgc cgggctgccc gcactgccgc tgacgatcgt
    16141 cccgctgtac ttcgagctcg acggcacctt cccgaaccac gaggcgaacc cgctcgaccc
    16201 gaagaacctc gtcgacctgc aggccgccgt ggtcgagcac ggcgctgatc tcgggttggc
    16261 cttcgacggc gacgccgacc gctgcttcgt cgtcgacgag cgcggtgagc cggtcagccc
    16321 gagcgccatc accgccctca tcgcgcgccg cgaggtggcg cgcgcggtcg cgcagggggc
    16381 cacggcatcc gacgtggcga tcgtccacaa cgtcatctgc tcggcccagg tgcccgaggt
    16441 catcgcggag accggcgccc gggcggtgcg cacccgggtc ggccactcct tcatcaaggc
    16501 cgagatggcg cgccacgacg cggtcttcgg cggcgagcac agcgcgcact actacttccg
    16561 tgacttctgg ttcgccgaca ccggcatgct cgccgcgatg cacacgctcg ccgcgctcgg
    16621 ggagcaggac gcgccgctga gtgtcgtcat gggcgacctg caccggtacg ccgcgtccgg
    16681 cgagatcaac tccacggtct cggatgccgc ggccgccacc gtgcgcgtgc gcgcctgggc
    16741 ggagggggag ggcgtcgtgg ccgatgagct cgacggtctc acgctcaccc acgccgggga
    16801 cggcgcgggc gacccgatgt ggtggctgaa cctgcgggcc tcgaacaccg agccgctgct
    16861 gcggctgaac gtcgaggccg ccgacgtggc cacgatggag cgggtgcgcg acaccgcgct
    16921 cgccctcatc cgcgcctgac ggggtgtggg aggatcaggg ttgatcgaca ccacccccgg
    16981 aaggacctca tgagcgagca ggcaaccccc gcgtcggcga tcgagccgtg gctgcgcgag
    17041 atcctgcgct gccccgcgtg ccgcagcgag ctgcgcgacg acaccggccc caccggtggc
    17101 cccgagctgg tctgcacctc gggtgagtgc gggctcgcgt accgcatcga cgacggggtg
    17161 ccggtgctgc tcgtcgacga ggcccgc
//
LOCUS       DAOSOC010000036        16800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3445_length_16800_cov_10.243715, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000036 DAOSOC010000000
VERSION     DAOSOC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 16800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16800
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3445_length_16800_cov_10.243715"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(570..1604)
                     /locus_tag="PL014_04685"
     CDS             complement(570..1604)
                     /locus_tag="PL014_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT56419.1"
                     /translation="MSVTTILLTVALLAANAFFVGAEFAAMSARRSQLEPLAEAGSKR
                     AQTALDAMQHTGILLATCQLGITVCSVLLGAISEAALHHALTPIFEDLGLSSVVADVV
                     ALGLALGLIVFLHVVYGEMIPKNISIARPERSALALVPPLVMVSKVVAPIIHAMDWLS
                     KALIRLFGVEPKDELATAFTAEEVAHILNESRDEGLVEDEHYERLGAALEFSDKDAAD
                     VGVPVSSLVTVGPEATPADIERLVAKHGFSRFPVRDDAGDLGGYLHLKDVLYADESER
                     GEPVPAKRVRRMANVRRDDEVEMVLGVMQATGSHLARVVAADGSVVGVVFLEDVIEEL
                     VGEVSDASQR"
     gene            complement(1601..2965)
                     /locus_tag="PL014_04690"
     CDS             complement(1601..2965)
                     /locus_tag="PL014_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT56420.1"
                     /translation="MTEWLLLLLGVVLTVGTAFFVAAEFSLVALDRPTVQKAVDAGEK
                     GARSVLTSHRQLSTQLSACQLGITLTTLILGFIASPSIGALLTGPLSSLGLSEAVAAS
                     TASVLAMVMATLFSMIVGEMVPKTLAISLPLATAKISAAPVRWFGISMKPMIALLNGV
                     ANRTLRALGIEPQEELSAARSPAELASLIRSSAEAGTLDVGTARLVTASLGFADQTAA
                     DVMTPRSRGTSIDRVATAAELVALARRTGHSRFPVIGSDWDDIDGIVHVKKAIAVPYD
                     RRGDVPVSALMVPPVLVPETLRLDPLLVQLREGGMQLAVVVDEYGGTSGIVTLEDLVE
                     EIVGEVSDEHDRSQTTARALKDGSWTVPGLWRPDEVRSRVGAAVPEGAAYETIGGWVM
                     AELGRVPMVGDEVVVDGWSVRVLDMDGHRVDRLRLAPPVVPPGEDAVRSGSAAARTHA
                     GGAA"
     gene            3112..4089
                     /locus_tag="PL014_04695"
     CDS             3112..4089
                     /locus_tag="PL014_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT56421.1"
                     /translation="MSATTSDGARIEVRGLTKRFGSFVAVDDLSFDVEPGRITGFLGP
                     NGAGKTTSLRMILGLVNATSGTATIDGHRYVDLPNPLGTVGAALEATNFHPGRSGRDH
                     LRVLADAGSIPLTRVDELLELTGIPAAARKRAGEYSMGMRQRLGLAAALLGDPKVLVL
                     DEPSNGLDPEGIRWLRGFLRHLSSEGKTILVSSHLLTEVQQTVDEVVIIANGRLVRSG
                     SIADLEGTRGAVVRTSDAGRLAGALRVADVTSTIDADGTLLADTTDLRLIGDVALRAG
                     LPIYGLQPSKADLEALFFQLTEGTNRNEVHGVGAPAEQDAVIGREGANL"
     gene            4086..4883
                     /locus_tag="PL014_04700"
     CDS             4086..4883
                     /locus_tag="PL014_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT56422.1"
                     /translation="MIPAIRSEFRKFFTTRLWWGMAVGIFLAGAAFATLFGFLLTSEA
                     ALAGTPGAPTGDATQIANSVYLGGLGVGYLLLLTIGVLQIGGEYRHKTITSTLLSTPK
                     RLKAMLAKVVALLGIGVVYGLISLLGSVSVGAIILNLRGQDPFPSTAVLRALALSLLV
                     LGLWALIGLGIGILIPNQVAALLIGIGVAWIVEPLLNFAMTFWEFGRENIAQYMPTSA
                     TNAVVNAVQSSPDEVRLEWWAAALVLAGYAALLSGFGIWRTTRADIS"
     gene            5047..8922
                     /locus_tag="PL014_04705"
     CDS             5047..8922
                     /locus_tag="PL014_04705"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HOT56423.1"
                     /translation="MAEQSPSSDPLTTFGPNEWLVDELYEQYQKDKNSVDRAWWDFFK
                     DYAPGEAALGRGAAAAATRAAPAAAAPAAPAAAAPAPAAPPAAPPANGSAPAAKAATP
                     PPAAAPAPAAAAAPAASAPAPAPARSEPAAAASTPKPREAAAPQASGPLGDAAQTPIR
                     GASARVVTNMESSLEVPTATSVRAVPAKLLIDNRIVINNHLARSRGGKVSFTHIIGYA
                     LVKALGVVPAMNNGFGHDAAGKPVLITPAHVNLGLAIDLAKPDGTRSLLVPSIKAAET
                     MDFAHFWTAYEEMVKKARAGKLTVEDFQGTSISLTNPGTIGTVHSVPRLMQGQGTIIG
                     VGALEYPAEWAGASLETLNRNAVSKILTLTSTYDHRIIQGAQSGDFLRIVHSLLLGEH
                     GFYDEIFESLRIPYEPIRWVQDVDTSHDDDINKVARVQELIHAYRVRGHLMADTDPLE
                     YKQRRHEDLDVTTHGLTLWDLDRTFATGGFGGQPFLKLRKILGILRDSYCRTIGVEYM
                     HIQDPEQRTWIQQKIEVGYAKTTREEQLRILRRLNAAEAFETFLQTKFVGQKRFSLEG
                     GESVIALLDRVLSRAAEDKLHEVCIGMPHRGRLNVLANIAGKSYGQIFREFEGKQDPR
                     SVQGSGDVKYHLGTEGTFTGEDGSTTKVYLAANPSHLEAVNPVLEGIVRAKQDRLNLA
                     GEDFTVLPVLLHGDAAFAGQGVVAETLNLSQLRGYRTGGTVHVVINNQVGFTTSPSSS
                     RSSTYSTDVARMIQAPIFHVNGDDPEACVRVAELAYEFRKEFRKDVVIDMVCYRRRGH
                     NEGDDPSMTQPLMYNLIEAKRSVRKLYTESLIGRGDITVEDAGVVLRDYQQQLERVFV
                     ETKAALKEAEAEPADTAYSGTDVEGHTGLEPPAAQTRGADRTKHSSTHTAISPEMLTR
                     LGDAFIDIPAGFTVHPKLKQMLEKRAASTREGGIDWGTGELLAFGSLLIDGTPVRLAG
                     QDSRRGTFVQRHAVLIDKENAAEWTPLLYLGEGQGKFWVYDSLLSEFAAMGFEYGYSV
                     ERPDALVLWEAQFGDFFNGAQTIVDEFISSSEQKWGQRSSVVLLLPHGYEGQGPDHSS
                     ARIERFLTMCAEGNMTVAYPSTPASYFHLLRRQAYARPRRPLIVFTPKSMLRLKAASS
                     APEDFTTGGFRPVMGDTPGATSGEVTRVLIAAGKVVYDLLAERDQRGDTSTAILRLEQ
                     YYPLPAADLAEELAKYPGAEVVIVQDEPKNQGAWPFLALNLPEALQALGENRMPTVVC
                     RPASASPATGSTKKHQIEQADLIARAFDR"
     gene            8994..9170
                     /locus_tag="PL014_04710"
     CDS             8994..9170
                     /locus_tag="PL014_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002518904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HOT56424.1"
                     /translation="MYFTDRGIEELQTRRGDEEVTFDWLAEQLRTFVDLHPDFEVPVE
                     RLATWLARLDDEDE"
     gene            9178..9849
                     /locus_tag="PL014_04715"
     CDS             9178..9849
                     /locus_tag="PL014_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT56425.1"
                     /translation="MSYELPDEGQAPIEFRPSAEAQVVAEGPRDLALVFLGGSLVAGV
                     GDPKGQGWVTRVVGRTQHPDLELTAYNLGVRGDTSADVLHRWTTEVPSRWSGRSERRI
                     VVSVGTNDVLQGISLARHRLNLANLLDDAATAGVGTFVVSPPPTDDADLNSRLEVLVE
                     AQADVCGRRDIPFVDSFTPLAGHDQWRTDLAASRVAHHPGQAGYGLIAWLVLHHGWGP
                     WLQIS"
     gene            complement(9859..10710)
                     /locus_tag="PL014_04720"
     CDS             complement(9859..10710)
                     /locus_tag="PL014_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT56426.1"
                     /translation="MNATSTEHTVTLHGHTFSYSDSGSGPALLFIHGLLGSQRQWTHL
                     VDRLDEDHRVIVPDLFGHGESAKPMGDYSLGAHAATLRDLLDRLEIEQVTLVGHSLGG
                     GIAMVFSYLFPERVDRLVLVASGGLGREVSPLLRSATLPGAEYVLPVIASGWMRDRLA
                     SAGRALQKVGVHPGSDITQVWHGFTSLGDADTRRAFLATTRAVIDPGGQSVTAHDYLP
                     EVAPTPTLVVWGTKDRMIPAWHAASATTSIPECRVELFQGAGHFPHLDDPDRFADLVR
                     EFVTLPA"
     gene            complement(10788..11756)
                     /locus_tag="PL014_04725"
     CDS             complement(10788..11756)
                     /locus_tag="PL014_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT56427.1"
                     /translation="MLAVTCTSQSTSDPLSGLTVGERPEPSVPDGWTTVRLRTAALNH
                     HDVWSLRGVGLPADRLPMVLGCDGAGVDADGNEVLVHAVVSSPGWAGDETLDPRRSLL
                     SEVYDGTLAEVVAVPARNLVPKPAGLSWEHAACLPTAWLTAYRMLFTNADVQPGSTVL
                     VQGAAGGVATALVQLGSAAGLRMWVTSRDEARGEQALALGADQAFSSGARLPERVDAV
                     METVGAATWSHSVNSLRPGGTIVISGATSGDAPAKAELTKIFFKQLRVVGSTMGTRDE
                     LERLSRFVVDAGIEPLVDSALPLADAREGFEAMVAGRVSGKVVFTI"
     gene            complement(11816..12667)
                     /gene="rfbD"
                     /locus_tag="PL014_04730"
     CDS             complement(11816..12667)
                     /gene="rfbD"
                     /locus_tag="PL014_04730"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOT56428.1"
                     /translation="MARWFVAGAAGMLGQDLCAVLEDAGHVVTRGDLPGLDILDPAQC
                     AEQVAGHDVVVNCAAYTAVDAAESDEARAFAVNAVGAANLATAAERAGAAMVQISTDY
                     VVAGSGDEPYAADAPVAPASAYGRTKAAGEWAVRANCSRSWIVRTAWLYGAGGKNFPA
                     TMQRLAGERETLTVVADQVGQPTWTVDLAEAIVRIVDAGAPFGIWHGTSSGECSWFDL
                     ARAVFEELGLDPQRVTPIATEDFPLPAPRPAYSVLSHDMWETAGVQPLPHWRDALHRS
                     APTVLAP"
     gene            complement(12667..13674)
                     /gene="rfbB"
                     /locus_tag="PL014_04735"
     CDS             complement(12667..13674)
                     /gene="rfbB"
                     /locus_tag="PL014_04735"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOT56429.1"
                     /translation="MKVLVTGGAGFIGSNFVHLTLATRPDVQVTVLDALTYAGSTDSL
                     SGVGDRVTFVEGDVADAAVVDPLVADADVVVHFAAESHNDNSLEDPSPFVRTNLIGTF
                     TLLEAARRHDVRYHHISTDEVYGDLELDDPKKFTEDTPYNPSSPYSSTKAGSDLLVRA
                     WVRSFGLRATISNCSNNYGPRQHVEKFIPRQITNVLDGVRPKLYGAGLNVRDWIHVDD
                     HNEAVWTIIDKGVIGETYLIGADGEVNNRDVVRMILELMGQEPDAYDHVTDRAGHDMR
                     YAIEAGRLRSELGWSPRYESFRDGLAATIEWYRENEAWWRPQKDATEARYARTQKVVA
                     P"
     gene            13730..14608
                     /gene="rfbA"
                     /locus_tag="PL014_04740"
     CDS             13730..14608
                     /gene="rfbA"
                     /locus_tag="PL014_04740"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOT56430.1"
                     /translation="MKGIILAGGSGTRLHPITRGISKQLMPVYDKPMVYYPLSTLMMA
                     GIREVLLITTPQDLEQFQRLLGDGSAWGIELTYAVQPSPDGLAQAFVIGADFIGSDSV
                     ALVLGDNIFHGTGLGTALRSLTDVRGGHIFAYHVSEPSAYGVVEFAADGTVLSIEEKP
                     ENPRSEYAVPGLYFYDNDVVEIARSLKPSPRGELEITAVNETYLRRGDLTVTVLPRGT
                     AWFDTGTFEGLMDASQFVHVVEARQGYKIGCVEEIAWRAGWIDDEAFAALGDDLLKSG
                     YGTYLHACLARGRSGS"
     gene            14608..15216
                     /locus_tag="PL014_04745"
     CDS             14608..15216
                     /locus_tag="PL014_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOT56431.1"
                     /translation="MHIEPLSIAGAFVVTPRQFPDDRGVFLESFRGDRLAEHLGHRPD
                     IVQTNVSVSARGTVRGIHFADVPPGQGKYVTALTGSLVDYVIDVRVGSPTFGQWEAVT
                     LDTTDRRAVYLSEGLGHAFCALEDDTTAMYLCTATYNPTGEHGIHPLDAEVGLTLPDG
                     IEPLLSAKDEAAPTLADAAASGLLPTYAACTAWAATLAERPR"
     gene            complement(15273..16442)
                     /locus_tag="PL014_04750"
     CDS             complement(15273..16442)
                     /locus_tag="PL014_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOT56432.1"
                     /translation="MAASPLVPFVDPESPVFRAHEGGKLGVHATKPLRDRDDLSLLYT
                     PGVAEVSLAIAADPSLSSRYTARGNTVAVISDGTAVLGLGDIGPLGAMPVMEGKAVLF
                     KHFAGIDAVPVVMETGTVDELVDAIARIAPSYGGINLEDISAPRCFDIEEQLKERLDI
                     PVFHDDQHGTAIVVLAGLLNAVRVLDRRLPEQRVVVSGAGAAGVAVTKLLQRAGVRDV
                     VVCDSRGIISPSRQDLTGHKSRLAASTNPRGLSGRISTALTGADVYIGVSGGTVPEDE
                     VALMAPGGMIFALANPTPEVHPDVAHRHAAIVATGRSDFPNQINNVLAFPGIFRGALD
                     SGAPRITEAMKLAAAHAIADLVEEPSAECIVPSVFQEGVTDSVASAVVALARSGR"
BASE COUNT         2586 a         6039 c         5805 g         2370 t
ORIGIN      
        1 cgggaggggg cgcaacttgc cgaaaccgcg ccgggacgcc ctccgcgctc gtggtggcat
       61 gctccgccgt gcctctcatc gtgccctaac cgtgccccaa cagtgtccta ggttgggttg
      121 ttgtgtccat cagtgtacct aggctagagt cggcatcagc gcagtacaga gggtgtttcg
      181 gcaggtcagc agcaccgacc acggttcatc ggtagagagt ttcgaatcct gccgggggca
      241 ccagccgcac acgcgccagc gcgggcggag gggaggaccc cggcagagat catgggagcc
      301 ggagcagaac gcgacgaggg agcgggctgc gacggcggct cctgccgggg gcaccacacc
      361 gccattgtcg gctggcaggc ccgccgatca ccgccggcgc ccggccggct gcgccgtcgg
      421 acgtgcaaaa gggctccctc ctgcccaggt aagcccgcgt tgcacgctca gtcgacgacc
      481 ctgcatcggg cattgcacgt ccggcggagc gccccgcccg gctgcgcagc aggaagccgg
      541 gcggtgggcc cggaccgcgg cgggaaggat cagcgctgcg aggcgtccga gacctcgccg
      601 acgagctctt cgatgacgtc ctcgaggaac accaccccca cgaccgagcc gtcggccgcg
      661 acgacccggg ccaggtgcga gccggtggcc tgcatgaccc cgagcaccat ctcgacctcg
      721 tcgtcgcggc ggacgttggc catccggcgc acgcgcttgg ccggaaccgg ctcgccccgc
      781 tccgactcgt cggcgtacag cacgtccttg aggtgcaggt agccaccgag gtcgcccgcg
      841 tcgtcgcgca ccgggaagcg ggagaacccg tgcttggcca cgagccgctc gatgtcggcc
      901 ggggtcgcct cggggcccac ggtcaccaac gacgacacgg gcacaccgac gtcggcggca
      961 tccttgtcgc tgaactccag cgcagcgccg agccgctcgt agtgctcgtc ctcgaccagc
     1021 ccttcgtcgc gcgactcgtt gaggatgtgc gcgacctcct cggcggtgaa ggccgtggcc
     1081 agctcgtcct tgggttcgac gccgaacagc cggatcaacg ccttggacag ccagtccatc
     1141 gcgtggatga tcggcgcgac gaccttggag accatcacca gcggtggcac gagggccagg
     1201 gcggagcgct ccgggcgggc gatcgagatg ttcttgggga tcatctcgcc gtagacgacg
     1261 tgcaggaaga cgatgaggcc gagggccagg ccgagagcca cgacgtccgc gacgaccgac
     1321 gagaggccga ggtcctcgaa gatcggcgtc agcgcgtggt gcagcgcagc ctccgagatg
     1381 gcaccgagca gcacggagca cacggtgatg ccgagctggc acgtggcgag caggatgccg
     1441 gtgtgctgca tggcgtccag tgccgtctgt gcccgcttgc tccctgcctc ggcgaggggc
     1501 tcgagctggc tgcggcgggc ggacatcgcg gcgaactcgg caccgacgaa gaaggcgttg
     1561 gcggcgagca gcgccacggt gagcaggatc gtggtcacac tcatgccgca ccccccgcgt
     1621 gggtgcgggc ggcagcggag ccgctgcgga cggcatcctc acccggtggc accacgggtg
     1681 gcgccaggcg aagtcggtcg acccggtgcc cgtccatgtc gaggacgcga acgctccagc
     1741 cgtcgacgac gacctcgtca ccgaccatgg gcacgcggcc gagctcggcc atgacccacc
     1801 cgccgatggt ctcgtaggcc gcaccctcgg gcacggcggc cccgacccgc gagcgcacct
     1861 cgtcggggcg ccagaggccg ggaacggtcc acgagccgtc cttcagggcc cgcgccgtgg
     1921 tctgggagcg gtcgtgctcg tcgctgacct caccgacgat ctcctcgacg aggtcctcga
     1981 gcgtcacgat gcccgacgtg ccgccgtact cgtcgacgac gacggcgagc tgcatgccgc
     2041 cctcccgaag ctggacgagc aacgggtcga ggcgcagcgt ctcggggacg agcacgggtg
     2101 ggaccatcag cgccgagacg ggcacgtcgc cgcgacggtc gtacggcacg gcgatggcct
     2161 tcttcacgtg cacgatgccg tcgatgtcgt cccagtccga cccgatgacg gggaagcggg
     2221 agtggccggt gcgacgggcc agggccacca gctcagccgc ggtcgccacc cgatcgatcg
     2281 aggttccccg cgagcgcggc gtcatgacgt cggcggcggt ctgatcggca aaaccgagcg
     2341 atgccgtgac gagcctcgcg gtgccgacgt cgagcgtgcc cgcctcggcg gaggaccgga
     2401 tgagcgatgc cagctcggca gggctgcggg cggcggagag ctcttcctgc ggctcgatgc
     2461 cgagcgcgcg cagggtgcgg ttcgcgaccc cgttgagcag ggcgatcatc ggcttcatcg
     2521 agatgccgaa ccaccgcacc ggcgcggcgg agatcttcgc ggtcgccagc ggcagggaga
     2581 tggcgagcgt cttgggcacc atctcgccga cgatcatcga gaacagcgtc gccatgacca
     2641 tggccagcac cgaggccgtc gacgcggcga cggcctcgct gaggccgagg ctgctcagcg
     2701 gcccggtgag cagggcaccg atggacgggc tcgcgatgaa gccgaggatg agcgtggtca
     2761 gcgtgatgcc gagctggcac gcggacagct gcgtggagag ctgccggtgc gaggtgagga
     2821 cggaccgggc ccccttctca ccggcgtcga cggccttctg cacggtgggc cggtcgaggg
     2881 cgacgagcga gaactccgcc gccacgaaga aggcggtgcc gaccgtgagg acgacgccga
     2941 ggagcagcag caaccattcg gtcatagtcg cgccatcgta ggcgagcagg gaccctcgcc
     3001 cggaatcccg ggacaccgct ccaccgcgag gtggacccgg ttccaccgcc gggttgaccc
     3061 cctcgccacg tgagcctgac aggatcgagg catgacacca cgagtgcgcg gatgagcgcc
     3121 accaccagcg acggtgcacg aatcgaggtc cgggggctca cgaagcgctt cgggtccttc
     3181 gtcgccgtcg acgacctcag cttcgacgtc gagcccggcc ggatcaccgg cttcctcggg
     3241 cccaacggcg ccggcaagac caccagcctg cgaatgatcc tcggcctcgt gaatgccacc
     3301 agcggcacgg ccacgatcga cgggcaccgg tacgtcgacc tgccgaaccc cctgggcacc
     3361 gtgggggcgg cgctcgaggc cacgaacttc cacccgggcc gcagcggccg cgaccacctg
     3421 cgcgtgctgg ccgacgccgg ctcgatcccc ctcacgcgcg tcgacgagct gctcgagctc
     3481 accggtatcc ccgccgcggc ccgcaagcgc gcgggtgagt acagcatggg catgcgccag
     3541 cgcctcggcc tggcagccgc gttgctcggc gacccgaagg tgctcgtcct cgacgagccg
     3601 tccaacggcc tggaccccga gggcatccgc tggctgcgcg ggttcctgcg ccacctcagc
     3661 agcgagggca agacgatcct cgtctccagc cacctgctga ccgaggtgca gcagaccgtc
     3721 gacgaggtcg tcatcatcgc caacgggcgg ctcgtccgct cgggctcgat cgctgacctc
     3781 gagggcaccc ggggcgccgt cgtgaggacc tcggatgccg gccggctcgc cggcgcgctt
     3841 cgcgtcgccg acgtcacgag caccatcgac gccgacggga cgctcctcgc cgacaccacg
     3901 gacctgcgcc tcatcggtga cgtcgccctg cgggccgggt tgccgatcta cggtctccag
     3961 ccgagcaagg ccgacctcga ggccctgttc ttccagctca ccgagggcac caaccgcaac
     4021 gaggtccacg gggtcggggc acctgccgag caggatgccg tcatcggccg ggaaggggcg
     4081 aacctgtgat ccccgcgatc cgcagcgagt tccgcaagtt cttcacgacc cgtctctggt
     4141 ggggcatggc cgtcggcatc ttcctcgccg gcgccgcctt cgccaccctg ttcgggttcc
     4201 tcctgacgag cgaggccgcg ctcgcgggga cccccggcgc cccgaccggc gacgccaccc
     4261 agatcgcgaa ctcggtctac ctgggcgggc tgggtgtcgg ctacctgctc ctgctcacca
     4321 tcggcgtgct gcagatcggt ggcgagtacc gccacaagac gatcacgtcg accctgctgt
     4381 ccacgcccaa gcgcctcaag gccatgctcg ccaaggtggt ggccctgctc ggcatcggcg
     4441 tcgtctacgg cctcatcagc ctgctcggat cggtcagcgt cggcgcgatc atcctcaacc
     4501 ttcgcgggca ggacccgttc ccgtccaccg ccgtgcttcg ggctcttgcc ctgagcctgc
     4561 tcgtgctcgg gttgtgggcg ctgatcgggc tggggatcgg catcctcatc cccaaccagg
     4621 tcgcggcgct gctcatcggc atcggtgtcg cctggatcgt ggagccgttg ctgaacttcg
     4681 cgatgacctt ctgggagttc ggccgcgaga acatcgccca gtacatgccg acgtccgcca
     4741 ccaatgccgt ggtcaacgcg gtgcagtcat cacccgacga ggtccggctc gagtggtggg
     4801 cagccgcgct ggtactggcc gggtacgcgg cgctgctctc cgggttcggt atctggcgca
     4861 cgacccgggc cgacatcagc tgagacccgt cagcgccggt gcgacggccg gtatgccgcc
     4921 cgtgcggcat accggccgtc gcacgtggtc aaccgctcat cctttcgggc cgctgtcgcc
     4981 ccgggcgcgg ggatacccta ggccggacca ccctcagagc cgagcccaga aagaggcgca
     5041 tcctccgtgg ccgagcagtc cccgtccagc gaccccctca cgaccttcgg acccaacgaa
     5101 tggctggtcg acgagctgta cgagcaatac caaaaggaca agaacagcgt cgaccgagcg
     5161 tggtgggact tcttcaagga ctacgcaccg ggtgaggccg cgctgggtcg gggcgccgcg
     5221 gccgcggcca cccgtgccgc gccggctgct gctgctcccg ctgctccggc tgccgccgca
     5281 ccggctcctg cggccccgcc cgccgcaccg cctgccaacg gttcggcgcc ggctgccaag
     5341 gccgccactc ccccacccgc agcggcgccc gcgcctgcag cggcagccgc gcccgcggca
     5401 tccgcgcctg cccccgcgcc cgcccgcagc gagcccgcgg ctgcggcatc cacccccaag
     5461 cctcgcgagg cggccgcgcc ccaggcctct ggtccgctcg gcgacgccgc ccagaccccg
     5521 atccgcgggg cgagcgcccg ggtcgtcacc aacatggagt cctcgctcga ggtgcccacc
     5581 gcaacgtccg tgcgagcggt cccggcgaag ctgctcatcg acaaccgcat cgtcatcaac
     5641 aaccacctcg cccgcagccg cggcggcaag gtgagcttca cccacatcat cggctacgca
     5701 ctcgtcaagg ctctgggtgt cgtgcccgcg atgaacaacg gcttcggcca cgacgccgcg
     5761 ggcaagccgg tgctgatcac cccggcgcac gtcaacctcg gccttgccat cgacctggcc
     5821 aagcccgacg gcacccgcag cctgctcgtg ccgtcgatca aggccgccga gacgatggac
     5881 ttcgcccact tctggaccgc ctacgaggag atggtcaaga aggcccgtgc gggcaagctg
     5941 acggtcgagg acttccaggg cacgtcgatc tcgctgacca accccggcac catcggcacg
     6001 gtgcactcgg tgccgcggct catgcagggc cagggcacga tcatcggcgt cggggcactc
     6061 gagtacccgg ccgagtgggc cggcgcgagc ctggagacgc tcaaccgcaa cgccgtgagc
     6121 aagatcctca cgctgaccag cacctacgac caccgcatca tccagggcgc ccagtccggc
     6181 gacttcctgc gcatcgtgca ctcactgctg ctcggcgagc acggcttcta cgacgagatc
     6241 ttcgagagcc tgcgcatccc ctacgagccg atccgctggg tgcaggacgt cgacacctcg
     6301 cacgacgacg acatcaacaa ggtcgcgcgg gtccaggaac tcatccacgc ctaccgcgtg
     6361 cgcggccacc tcatggccga caccgacccg ctggagtaca agcagcgccg ccacgaggac
     6421 ctcgacgtca cgacccacgg actcacgctg tgggatctcg accgcacctt cgcgaccggc
     6481 ggcttcggcg gccagccgtt cctgaagctg cgcaagatcc tgggcatcct ccgcgactcg
     6541 tactgccgca ccatcggcgt ggagtacatg cacatccagg acccggagca gcgcacgtgg
     6601 atccagcaga agatcgaggt gggttacgcc aagaccaccc gtgaggagca gctgcgcatc
     6661 cttcgccgcc tgaacgcggc cgaggcgttc gagaccttcc tgcagaccaa gttcgtcggg
     6721 cagaagcgct tctccctcga aggtggcgag tccgtcatcg ccctgctcga ccgggtgctc
     6781 tcacgagccg ccgaggacaa gctgcacgag gtctgcatcg ggatgccgca ccgcggccgc
     6841 ctgaacgtcc tcgccaacat cgcgggcaag tcctacgggc agatcttccg tgagttcgag
     6901 ggcaagcagg acccccgctc ggtgcagggt tcgggtgacg tgaagtacca cctcggcacc
     6961 gagggcacct tcaccgggga ggacggctcg accaccaagg tctacctcgc cgccaacccc
     7021 tcccacctcg aggccgtcaa cccggtgctc gagggaatcg tgcgggccaa gcaggaccgc
     7081 ctcaacctcg cgggtgagga cttcaccgtc ctgcccgtgc tgctgcacgg tgacgccgcc
     7141 ttcgctggcc agggagtcgt cgccgagacg ctcaacctct cgcagctgcg cggctaccgc
     7201 accggcggaa cggtgcacgt cgtcatcaac aaccaggtcg gcttcaccac ctcgccgagc
     7261 tcgtcacggt cctcgacgta ctccaccgac gtggcccgga tgatccaggc gccgatcttc
     7321 cacgtcaacg gcgacgaccc cgaggcgtgc gtgcgcgtgg ccgagctcgc ctacgagttc
     7381 cgcaaggagt tccgcaagga cgtcgtcatc gacatggtct gctaccgccg ccgcggtcac
     7441 aacgagggcg acgacccgtc gatgacccag ccgctgatgt acaacctcat cgaggccaag
     7501 cgcagcgtcc gcaagctcta caccgagtcc ctgatcggcc gtggcgacat cacggtcgag
     7561 gatgccggcg tggtgctgcg cgactaccag cagcagctcg agcgggtctt cgtcgagacc
     7621 aaggcggcgc tgaaggaagc cgaggccgag ccggccgaca cggcgtactc gggcaccgac
     7681 gtcgagggcc acaccggtct ggagccgccg gcggcacaga cccgcggcgc cgaccgcacc
     7741 aagcactcct cgacgcacac cgcgatcagc ccggagatgc tcacccgcct cggcgacgcc
     7801 ttcatcgaca tcccggccgg gttcacggtg caccccaagc tcaagcagat gctcgagaag
     7861 cgcgccgcga gcacccgaga gggtgggatc gactggggca ccggtgagct gctcgccttc
     7921 ggttccctcc tcatcgacgg cacccccgtg cgcctcgctg gccaggacag ccgccgcggc
     7981 accttcgtcc agcgccacgc ggtgctcatc gacaaggaga acgccgccga gtggactccg
     8041 ctgctctacc tcggtgaggg ccagggcaag ttctgggtct acgacagcct gctgtcggag
     8101 ttcgcggcga tgggcttcga gtacggctac tcggtcgagc ggcccgacgc cctcgtgctg
     8161 tgggaggccc agttcggcga cttcttcaac ggcgcccaga cgatcgtcga cgagttcatc
     8221 tcgagctcgg agcagaagtg gggccagcgg tcctcggtgg tcctgctgct cccccacggc
     8281 tacgagggac agggtccgga ccactcctcg gcccgcatcg agcgcttcct cacgatgtgc
     8341 gccgagggca acatgacggt ggcctacccc tcgaccccgg cgtcctactt ccacctgttg
     8401 cgtcgtcagg cctacgcccg tccccgtcgc ccgctcatcg tcttcacgcc gaagtcgatg
     8461 ctgcgcctga aggccgcgag ctcggccccg gaggacttca ccaccggcgg cttccggccg
     8521 gtgatgggcg acacgccggg ggcgacctcg ggcgaggtga cccgcgtgct catcgccgcc
     8581 ggcaaggtcg tctacgacct gctcgccgaa cgcgatcagc gcggtgacac ctcgacggcg
     8641 atcctgcgtc tggagcagta ctacccgctg cccgccgccg acctcgctga ggagttggcg
     8701 aagtacccgg gcgccgaggt cgtcatcgtc caggacgagc cgaagaacca gggcgcctgg
     8761 ccgttcctcg cgctgaacct gcccgaggcc ctgcaggcac tcggtgagaa ccggatgccc
     8821 acggtggtgt gccgcccggc atccgcctct ccggcgaccg ggtcgacgaa gaagcaccag
     8881 atcgagcagg ccgacctgat cgcccgcgcc ttcgaccgct gagggtcgac acgccggatg
     8941 ccgtgcgccg cccgggcgca cggcgtccgg ctgcgggcgg gtaacctcac agcgtgtact
     9001 tcaccgaccg tggtatcgag gagctccaga cccggcgcgg cgacgaggag gtcacgttcg
     9061 actggctggc ggagcagttg cgcacgttcg tcgacctcca cccggacttc gaggtgcccg
     9121 tggagcggct ggccacctgg ctggcccgac tggacgatga ggacgagtga cccgctgatg
     9181 tcgtacgaac tgcccgacga gggccaggcc cccatcgagt tccggccgag cgccgaggcc
     9241 caggtggtcg ctgaaggtcc gcgcgacctc gccctggtct tcctcggtgg ctcgctcgtc
     9301 gcgggtgtcg gggacccgaa aggtcagggg tgggtgaccc gcgtggtcgg ccgcacgcag
     9361 catcccgacc tcgagctcac ggcctacaac ctgggggtgc gcggcgacac ctccgccgac
     9421 gtcctgcacc ggtggaccac cgaggtcccg tcacggtggt cgggtcgcag cgagcggcgc
     9481 atcgtcgtct ccgtcggcac caacgacgtg ctccagggca tctccctcgc ccggcacagg
     9541 ctcaacctgg cgaacctgct cgatgacgcg gcgaccgcgg gggtcggcac cttcgtcgtc
     9601 agccccccgc cgaccgacga tgccgacctc aactcccgac tcgaggtgct cgtcgaggcg
     9661 caggccgacg tgtgcggtcg acgcgacatc ccgttcgtcg actcgttcac cccgctggcc
     9721 ggccacgacc agtggcgaac cgacctggct gcgagccggg tcgcccacca cccggggcag
     9781 gccggttacg gcctcatcgc gtggctcgtg ctgcaccacg gctggggccc gtggctgcag
     9841 atctcctgac gctcgcgctc acgcaggcag ggtgacgaac tcgcgcacca ggtcggcgaa
     9901 ccggtcgggg tcgtcgaggt gcgggaagtg ccccgccccc tgaaacagct cgacccggca
     9961 ctcggggatg gacgtcgtcg ccgatgccgc gtgccacgcg gggatcatcc ggtccttggt
    10021 gccccagacc acgagggtgg gcgtgggggc gacctcaggc aggtagtcgt gggccgtgac
    10081 gctctggcca ccggggtcga tgaccgcgcg ggtcgtggcg aggaaggccc ggcgggtgtc
    10141 agcgtcaccg agggaggtga aaccatgcca cacctgggtg atgtcggagc ccgggtgcac
    10201 cccgaccttc tgcagggcgc ggcccgccga ggccaggcgg tcgcgcatcc acccggaggc
    10261 gatgaccggc agcacgtact cagcgcccgg gagggtggcc gagcgcagca gggggctgac
    10321 ctcgcgcccg agaccaccac tggccacgag cacgagccga tcgacccgct cggggaacag
    10381 gtaggagaac accatggcga tgccgccgcc cagcgagtgc ccgacgagcg tcacctgctc
    10441 gatctcgagg cgatccagaa ggtcgcgaag ggtcgcagca tgcgccccga gggagtagtc
    10501 ccccatcggc tttgccgact caccgtggcc gaacaggtcc gggacgatga cgcggtggtc
    10561 ctcgtcgagc cggtcgacga ggtgggtcca ctggcgctgg ctcccgagca ggccgtggat
    10621 gaacagcagt gccgggccgc tgccgctgtc gctgtaggag aaggtgtgcc cgtgcagggt
    10681 caccgtgtgc tcggtggacg tcgcgttcac gatggctcct gatggcaggc atggggtggg
    10741 aacgcctcag gttaccgctg agtagcctcg cgtgtgaccg ggtgtgatca gatcgtgaag
    10801 acgaccttgc cgctcacccg cccggccacc atcgcctcga aaccttcccg ggcatccgcg
    10861 agcggcagcg cggagtccac gagcggctcg atgccggcgt cgacgacgaa ccgggacagc
    10921 cgttcgagct cgtcacgcgt gcccatcgtc gacccgacga cgcggagctg cttgaagaag
    10981 atcttggtca gctcggcctt ggccggtgcg tcccccgagg tcgcgccgga gatgacgatg
    11041 gtgcccccgg ggcgcaggct gttgacggag tgcgaccacg tcgcggcgcc gacggtctcc
    11101 atgacggcgt cgacccgctc ggggaggcgg gcgccgctcg agaacgcctg gtcggcaccc
    11161 agggcgagcg cctgctcacc gcgggcctcg tcgcggctgg tgacccacat gcgcagtccg
    11221 gcagccgagc ccagctgcac cagagcggtg gccacgccgc cggcggcccc ctggacgagc
    11281 acggtcgaac cgggctggac gtctgcgttc gtgaacagca tccggtaggc cgtgagccac
    11341 gcggtgggca ggcaggccgc atgctcccag gacagccctg cgggtttggg gacgaggttg
    11401 cgggctggca ccgcgacgac ctccgcgagc gtgccgtcgt acacctcgga gagcaggctg
    11461 cgccgcgggt cgagcgtctc gtcgccggcc cacccggggg aggagacgac cgcgtgcacg
    11521 agcacctcgt tgccgtcggc gtcgaccccc gcaccgtcac acccgagcac catcggcagc
    11581 cggtcggcag gcagccccac cccgcgcaag gaccacacgt cgtggtggtt gagcgccgca
    11641 gtgcgcagcc gcaccgtggt ccacccgtcg gggacactcg gctcgggccg ttcaccgacg
    11701 gtgaggccgc tcagagggtc ggacgtcgac tggctggtgc aggtcacggc gagcatgacc
    11761 tgaccctacg accccgccgc atcctgcgcc ccgttccggg tcacacggaa cgcgttcagg
    11821 gcgccaggac cgtcggggcc gaccggtgca gggcatcgcg ccagtgcggc aagggctgca
    11881 cgccggccgt ctcccacatg tcgtgcgaca gcacgctgta cgccggtcgc ggcgccggca
    11941 gcgggaagtc ctcggtcgcg atcggtgtca cccgctgcgg gtccaggccg agttcctcga
    12001 acaccgcacg cgccaggtcg aaccacgagc actcgccgga cgacgtcccg tgccagatgc
    12061 cgaagggggc acctgcgtcg acgatccgca cgatggcctc agcgaggtcg acggtccacg
    12121 tcggttgccc gacctggtcg gccacgacgg tcagggtctc gcgctcaccg gccagtcgct
    12181 gcatcgtcgc cgggaagttc ttgccgccgg cgccgtagag ccaggcggtg cgcacgatcc
    12241 acgagcgcga gcagttcgcg cgaaccgccc actcccccgc ggccttggtg cggccgtagg
    12301 cggatgccgg ggcgacgggt gcgtcggcgg cgtacggttc gtcccccgac ccggcgacga
    12361 cgtagtcggt cgagatctgg accatggcgg cgcccgcccg ctcggccgcg gtggcgaggt
    12421 tcgcggcacc gaccgcgttg acggcgaagg ctcgggcctc gtcgctctca gcggcatcca
    12481 ccgcggtgta cgcggcgcag ttcacgacga cgtcgtgtcc ggccacctgc tcggcacact
    12541 gggcggggtc gaggatgtcg aggccgggaa ggtcacctcg ggtgaccacg tgcccggcgt
    12601 cctcgaggac cgcgcacagg tcctgaccga gcatgcctgc ggcaccggcg acgaaccagc
    12661 gagccatcag ggggccacga ccttctgggt gcgggcatac ctggcctcgg tggcgtcctt
    12721 ctgggggcgc caccacgcct cgttctcgcg gtaccactcg atcgtcgcgg caagaccgtc
    12781 gcggaagctc tcgtagcggg gcgaccagcc gagttcggag cgcagccgac ccgcctcgat
    12841 ggcgtatcgc atgtcatgcc cggcccggtc ggtcacgtgg tcgtacgcgt cgggctcctg
    12901 gcccatcagc tcgaggatca tccgcacgac gtcgcggttg ttgacctcac cgtcggcccc
    12961 gatgaggtag gtctcgccga tgacgccctt gtcgatgatc gtccagaccg cctcgttgtg
    13021 gtcgtcgacg tggatccagt cgcgcacgtt gagtccggcg ccgtagagct tgggccggac
    13081 cccgtcgagg acgttggtga tctggcgcgg gatgaacttc tcgacgtgct ggcgcgggcc
    13141 gtagttgttg gagcagttcg agatcgtcgc gcgcagaccg aacgagcgca cccacgcccg
    13201 cacgagcagg tcggagccgg ccttggtcga ggagtagggc gagctcgggt tgtacggggt
    13261 gtcctcggtg aacttcttcg ggtcatcgag ctccaggtcg ccgtagacct cgtcggtgga
    13321 gatgtggtgg tagcgcacgt cgtgccggcg ggctgcctcg agcaaggtga aggtgccgat
    13381 gaggttggtg cgcacgaacg ggctcgggtc ctcgagcgag ttgtcgttgt gcgactcggc
    13441 ggcgaagtgc acgaccacgt cggcgtcggc gacgagcggg tcgacgacag cggcatccgc
    13501 gacgtcaccc tcgacgaacg tcacccggtc gccgaccccc gacagcgagt ccgtcgaccc
    13561 ggcgtaggtg agggcatcga gcaccgtgac ctgcacgtcg ggacgggtcg cgagggtgag
    13621 gtggacgaag ttgctgccga tgaacccggc gccgccggtg acgaggacct tcatggcgcc
    13681 aagggtagtg gcccgcgcgc cgtcggcccc gccgactagg ctcgggccca tgaagggcat
    13741 catcctcgcc ggcggatccg gcacgcgact gcacccgatc acccggggca tctcgaaaca
    13801 gctcatgccg gtctacgaca agccgatggt gtactacccg ctgtcgacgt tgatgatggc
    13861 cgggatccgc gaggtcctgc tcatcacgac cccgcaggac ctcgagcagt tccagcgcct
    13921 cctcggcgac ggcagcgcct ggggcatcga gctgacgtac gccgtgcagc cgagccccga
    13981 cgggctcgcc caggccttcg tcatcggcgc ggacttcatc ggcagcgact cggtcgccct
    14041 cgtgctcggc gacaacatct tccacggcac cggcctgggc accgcgctgc gctcgctcac
    14101 cgacgtgcgc gggggccaca tcttcgctta ccacgtgagc gagccctcgg cctacggcgt
    14161 cgtcgagttc gccgccgacg gaaccgtgct ctccatcgag gagaagcccg agaacccccg
    14221 ctcggagtac gccgtgccgg gcctgtactt ctacgacaac gacgtcgtcg agatcgcgcg
    14281 cagtctcaag cccagccccc gaggtgaact ggagatcact gcggtcaacg agacctacct
    14341 gcgccgcggc gacctcaccg tgaccgtgct gccccgcggt acggcgtggt tcgacaccgg
    14401 caccttcgag ggcctgatgg acgccagcca gttcgtccac gtcgtcgagg cccggcaggg
    14461 gtacaagatc ggctgtgtcg aggagatcgc ctggcgggcc ggctggatcg acgacgaggc
    14521 cttcgccgcc ctcggtgacg acctcctgaa gtcggggtac gggacctacc tgcacgcctg
    14581 cctcgcgcgg ggacggtcgg gctcctgatg cacatcgagc cgctgtccat cgctggcgcc
    14641 ttcgtcgtca ccccacggca gttccccgac gaccgggggg tcttcctcga atccttccgt
    14701 ggcgaccgcc tcgccgagca cctcgggcac cggccggaca tcgtgcagac caacgtctcg
    14761 gtctcggcgc ggggcacggt ccgtggcatc cacttcgccg acgtcccccc cgggcagggc
    14821 aagtacgtga cggccctgac cgggtccctc gtcgactacg tcatcgacgt gcgggtgggc
    14881 tcccccacgt tcgggcagtg ggaagcggtc accctcgaca ccaccgaccg gcgggcggtg
    14941 tacctctccg aggggctggg gcacgcgttc tgcgcgctcg aggacgacac cacggccatg
    15001 tacctctgca cggccaccta caacccgacc ggcgagcacg gcatccaccc gctggacgcc
    15061 gaggtcggcc tcaccctgcc cgacggcatc gagccgctgc tgtcggccaa ggacgaggcg
    15121 gccccgaccc tcgccgacgc ggcggcatcc ggcctcctcc cgacctacgc cgcgtgcacg
    15181 gcctgggcgg ccaccctcgc cgaacgcccc cgctgacggc ggacggacgg catccctggt
    15241 ccggatgccg tccgctcacc acccgggtca gctcacctgc cgctcctggc cagggccacc
    15301 acggctgagg caacggagtc ggtgacgccc tcctggaaca ccgagggcac gatgcactcg
    15361 gcactcggct cctcgacgag gtcagcgatc gcgtgggcgg ccgcgagctt catcgcctcg
    15421 gtgatccggg gggctccgga gtccagggcg ccacggaaga tcccggggaa ggccagcacg
    15481 ttgttgatct ggttcgggaa gtccgagcgg ccggtggcca cgatggccgc gtgccggtgc
    15541 gcgacgtccg ggtgcacctc aggcgtcggg ttggcgagcg cgaagatcat gccgcccggc
    15601 gccatgagag ccacctcgtc ctccgggacg gtgccaccgg acaccccgat gtagacgtcg
    15661 gcgccggtca gggcggtgct gatccggccc gagaggccgc gtgggttggt cgaggccgcc
    15721 agccggctct tgtggccggt gaggtcctgg cgggacggtg agatgatgcc gcgcgagtca
    15781 cagacgacga cgtcgcgcac gccggcacgc tggagcagct tggtcacggc gaccccggcc
    15841 gcacccgccc cggagacgac gacccgctgc tcgggcaggc gacggtccag cactcgcacg
    15901 gcgttcagca gccccgcgag gacgacgatc gccgtgccgt gctggtcgtc gtggaagacg
    15961 gggatgtcga ggcgctcctt gagttgctcc tcgatgtcga agcagcgcgg ggccgagatg
    16021 tcctcgaggt tgatgcctcc gtatgacggc gcgatgcggg cgatggcgtc gacgagctcg
    16081 tcgaccgtgc ccgtctccat gaccaccggc acggcgtcga tgccggcgaa gtgcttgaac
    16141 agcaccgcct tgccctccat gaccggcatg gccccgagcg ggccgatgtc gccgagaccg
    16201 agcaccgcgg tgccgtcgct gatgacggcc acggtgttgc cgcgtgcggt gtaccggctc
    16261 gacagggagg ggtcggccgc gatggccagc gacacctcgg cgacgccggg cgtgtagagc
    16321 agcgagaggt cgtcccggtc gcgcaacggc ttggtcgcgt gtaccccgag cttgccgccc
    16381 tcgtgagcac gaaagaccgg gctctccggg tcgacgaagg ggacgagggg cgacgcagcc
    16441 atgggaggca gctccttcgg ggcgacgcgt cagaagcaga cgcgcggggg gtggaagcgc
    16501 ccacgacgac ggtgggcggc gcggcatccg gggaaggggt gaggttccga acggctcgca
    16561 gggcgcgggg gtggctgcgc tgcgcgccat catgacacgc tcgccgggtc cagcacaggg
    16621 tccacggtgt gacccccgtc gcgtccccgg accacggaat ccgttgcgca tgttgttacc
    16681 gaggcgtgac gtgcgcaggg cccggcggtg gcagtatcgc cctcgtaccc ccgtgccgcg
    16741 tcgcacccct gcggcacgcc cccgtgaaag gacgcacatg ttccgcaccc gcgcgctggc
//
LOCUS       DAOSOC010000037        16602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3508_length_16602_cov_9.782438, whole genome shotgun sequence.
ACCESSION   DAOSOC010000037 DAOSOC010000000
VERSION     DAOSOC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 16602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16602
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3508_length_16602_cov_9.782438"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /gene="gatA"
                     /locus_tag="PL014_04755"
     CDS             <1..1220
                     /gene="gatA"
                     /locus_tag="PL014_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOT56433.1"
                     /translation="PILGKTNMDEFAMGSSTEHSAYGPSHNPWDLSRIPGGSGGGSSS
                     VVASFQAPLAIGTDTGGSIRQPAAVTGTVGTKPTYGGVSRYGLVALASSLDQAGPCTR
                     TVLDAALLHEVIGGHDPLDSTSIDAPVPPVVEAARRADVAGMKIGIIRELTGDGFQAG
                     VQARFDEAVQHLVDAGAEVVEVSCPSFSYALAAYYLILPSEASSNLAKFDAMRYGLRV
                     APDGIDAPSAEQVMAATRDAGFGDEVKRRIILGTYALSSGYYDAYYGSAQKVRRLIAD
                     DFAAAFETADVLVSPTAPTTAFRLGDKLDDPMAMYLNDIATIPANLAGIPGMSVPSGL
                     ATEDGLPAGFQILAPAMQDQRLYAVGAALEARLTAAWGGPLLDRAPDLLGNVRHLGPQ
                     DSHLAGRNAGTEA"
     gene            1224..2750
                     /gene="gatB"
                     /locus_tag="PL014_04760"
     CDS             1224..2750
                     /gene="gatB"
                     /locus_tag="PL014_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOT56434.1"
                     /translation="MTQTHAEATLPFDDAIAAFDPVMGLEVHVELNTATKMFCGCPTE
                     FGAEPNTQTCPVCLGLPGALPVVNAVAVESAIRIGLALNCEIAQWCRFARKNYFYPDM
                     PKNFQTSQYDEPIAFEGYLDVELDDGTVYRVEIERAHMEEDTGKSLHVGGSTGRIHGA
                     THSLVDYNRAGIPLIEIVTKPLTGAGERAPEIARAYVATLRDLLKALDVSDVRMEQGS
                     MRCDVNLSLMPKGTEVFGTRSETKNVNSLRSVERAVRYEVGRHAAVLSSGGTIVQETR
                     HWHEDTGVTTSGRAKSDADDYRYFPEPDLVPVAPSRELVESLRATLPEPPAARRKRLQ
                     GEWGFADLEMRDVVNAGLVDLVEETVAAGASPAGARKWWSGEVARRANAEGVDVPTYA
                     AGLGVGAASVVELEGLVTSGRLNDSMARQVWEGVLAGEGTPTQVADARGLELVQDTGA
                     LEAAVDAVIAANPDVADKIRDGKVAAAGALIGQVMKAMKGQADAGAARAIIMERLGQS
                     "
     gene            2993..3556
                     /gene="ahpC"
                     /locus_tag="PL014_04765"
     CDS             2993..3556
                     /gene="ahpC"
                     /locus_tag="PL014_04765"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HOT56435.1"
                     /translation="MSLVNTTIKPFEATAFVNGSFVPVSSADIEGKWAIFFFYPADFT
                     FVCPTELGDLADHYEELKALGVEVYSVSTDTHFTHKAWHEASDTIRKVTYPMIGDPTQ
                     RISKNFKILRKAEGLADRGTFLVDPDGVIQFHEVTAEGIGRNAAELVRKVKAAQYVRN
                     HPGEVCPAKWEEGEDTLAPSLDLVGKI"
     gene            3561..5153
                     /gene="ahpF"
                     /locus_tag="PL014_04770"
     CDS             3561..5153
                     /gene="ahpF"
                     /locus_tag="PL014_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HOT56436.1"
                     /translation="MLDPALAKQLSAYLTKVVDPVELVSSLDESATSGQLADLLDEIA
                     SLSAGRITHVRGGDAARRPSFRVVRVGTAVEVEFAGMPLGHEFTSLVLALLQVGGHPA
                     TAATELLDAVRGLTGEHRFETYFSQSCQNCPDVVQALNLMSILNPGISHVAIDGAVFP
                     GEVEERGVLAVPMVFRNGELFGQGRMTLEEIVARLDEGAAARDAQRLDELEPFEVLVV
                     GGGPAGSTAAIYTARKGIRTGLLAERFGGQLLDTMAIENYPSVEHTEGPRMAADLERQ
                     VRGLGVEVMTGQVAVRLVPASREPQGSGLHEVHLASGAVLRARAVVAASGARWRTLDI
                     PGEDEYRNRGVTFCPHCDGPLFAGKRVAVIGGGNSGVEAAIDLAGVVAHVTLVEFADE
                     LKADDVLQRKVHSLGNVDVLVGTATTEVVGDGSKVVGLRHAPRAGGASSVLDVDGVFV
                     QIGLLPTTEWLDGVVDLTPHGEVVVDARGATGIPGVFAAGDCTTAPWKQIVAAAGSGA
                     GAALSAFDHLIRTTSPASSPSA"
     gene            complement(5167..5844)
                     /locus_tag="PL014_04775"
     CDS             complement(5167..5844)
                     /locus_tag="PL014_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOT56437.1"
                     /translation="MSALDDILGALPADAISQQVGASPDEVRAAAAAVLPALLGGLQA
                     NASDPSGAGSILEALGQHQDDLLSGGADLSAINESDGQAITNHIFGDREEDVVNRLGE
                     VPAMGGSGMGGALVKKLLPILAPMVLSWLAKQVLGGAGGGGGGLGGGGAAPQRTDTAP
                     SLPGSTGGAAGGPGSLDDMLRDVLGGATGGAATAPSGGGAAAPGGLDAGSIIGDILGG
                     MLGGGRR"
     gene            5966..6496
                     /locus_tag="PL014_04780"
     CDS             5966..6496
                     /locus_tag="PL014_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT56438.1"
                     /translation="MTRAAQDRTLVLLRHAKAEQQNWDNDHERELTARGRRDAAAAGR
                     WLIEHGIGIDEVLCSTSERTQQTTEAVWSSGCPEGEVHLDRRIYLASPERLLEVIHEA
                     DEDADVLMVVGHAPGIPSLAELLCDGEGSVEGHRKMAEGFPTCAVAVLHYAGPWAGLQ
                     FGDATLERFHVCRDDD"
     gene            6561..6767
                     /locus_tag="PL014_04785"
     CDS             6561..6767
                     /locus_tag="PL014_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56439.1"
                     /translation="MSPTSRALVTTVAVVDAGLRAWALVDLRNRPADQVKGPKAVWGL
                     ALSVVSSAGVLPAAYLVLGRRPRG"
     gene            complement(6769..8163)
                     /locus_tag="PL014_04790"
     CDS             complement(6769..8163)
                     /locus_tag="PL014_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56440.1"
                     /translation="MSRPTGAAVTQPLSTDERAELERLRALVAAQATADPTDPATPAG
                     TAGGGPRSTRGRWAGSIVLMLLAALIAPLSALATWSNATLLDTDEYVAMVAPLAQDPA
                     IQDEISRRITDAVFEALQVEERTATTLAAIAERPRLEGVTDTLPLDITSFAVPISNAV
                     RGFTSEQVSRLVASDGFQDAWIAANREAHADLVAALTGQTSAGVSVTDGRVSVNLAAF
                     IEAIKPVLVERGVPFADRIPTVNAQFVVLDSANLGQAQTAVRLLDLLHTVLPVSAVLL
                     LVAGVALAPSRRRALVVGSSMAVGSLVMLLLGVQVLRALYLGDLEQALLVPEAGEVLF
                     DAVAMPLRLWVRASAVLFLLIAVGAWLAGPSSAARAARTLPSRLAGSTRQPTGALATG
                     ARFAAHNVSGLRAAVGGLAVLAILVGDRPSARGVLVIAVLVALALTAIEVLRRWGDDV
                     TVEDDAAEEAAAVG"
     gene            complement(8241..9260)
                     /locus_tag="PL014_04795"
     CDS             complement(8241..9260)
                     /locus_tag="PL014_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT56441.1"
                     /translation="MTGLTTAAARALDSTLDRTIAPGFTRLGYAVRRRLPTWPADPTP
                     GILTGHHVAVTGASSGLGERTAHDLAALGATVHLVVRDAGRGGRVAAEIESAVGRAGA
                     ARVWVCDVADLDSVRTFCSAFLAAGLPLRALVHNAGALPATRTESPQGHELTMALHVL
                     GPVLMTELLLPLLGADAGRVVFVTSGGMYSQPLPVDDPEFLRGEYSGTTAYARSKRGQ
                     IELLPVLALRWLPAGVSVSATHPGWAATPGVQTSLPRFNALLGPSLRTAGEGADTTTW
                     VVAADPAPASGQLWHDRRPRPTSIMRRTRPTAEQRGRFWEWVQEATGTGGSPERGEEG
                     RARRP"
     gene            complement(9257..9691)
                     /locus_tag="PL014_04800"
     CDS             complement(9257..9691)
                     /locus_tag="PL014_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT56442.1"
                     /translation="MVPGYRQSPSLVALQELVDVAGQVPHEVARQAGLSVSELHSLRH
                     LMATPMGPVELARELGVTSAASSGIVDRLVGRGHAQRRPHPDDGRRTEVVITDSGRVE
                     VLTRLAPMFRGLAEIDASLTDDERVVVERYLRGAVTAMKALM"
     gene            9822..11906
                     /locus_tag="PL014_04805"
     CDS             9822..11906
                     /locus_tag="PL014_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT56443.1"
                     /translation="MGNLAHAITRKWVAGILALVAILGAGAIIGVVGQAEGPPTAVAA
                     LPDGTDSKAAAELRAELPEAEGSAAVVLYSSDAALTPDQLAVVQERARTLPGATGAPP
                     AVAEDGTAATVFIPVNTSDAVETAEVVGDLREAAKADLPDGLTAQVTGPAAIQADLAA
                     VFDGANFRLLAATASVVALLLVITYRSPVLWLVPLTVVGVADQLAAVAATHTLAAFDV
                     LWDESTIGILSVLVFGAGTDYALLLISRYRDQLRVTEDRREAMAIALRRTTEAIVASS
                     STVVIGLLTLLLSLFPATRGLGLACAVGVVVAAFFVLVVLPAALVVFGRWIFWPKVPK
                     VGQAGLADANSLWRRIGDAVAKRPAGFVTVTLLALAVMAGGITQIKTGLDGPDQFLQK
                     PEAIAAAERLGESFPAGAADPTIVVTRGDGEATATAVAAVDGISDARVVASGNGVSEI
                     DAVIDAAPGSDEAEATVTALREALAGQADTHVGGTEAEAMDASASAARDRLVIFPIIL
                     ALVLVSLAALLRSVVAPIILVATVVATYMASLGLSWVLFTQVFGFERLDDGVPLLAFV
                     FLVALGVDYNIFLITRAAEEAKDHGSRSGMLRALAATGGVITSAGILLAAVFAVLGVL
                     PLVVLAQLGVIICVGVLLDTLVVRTVLVPAIGLILGDRFWWPRRVDTRPSVADAADST
                     DRAEGPVPVGGA"
     gene            11903..12382
                     /locus_tag="PL014_04810"
     CDS             11903..12382
                     /locus_tag="PL014_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOT56444.1"
                     /translation="MTLTRFGLGVTLAALIGLYAVLAVRWTGAGSAWATTLVRPAWQP
                     PDVVFGVVWPLNFIAMAVVGTAVTVGATPREGVLWMVLFGASVALALGWARAFYLRHR
                     LGRSAALLSLAAAVTWALVALTASTVGWPAWVLGPYAVWLTVASSLAVGYWRLNESR"
     gene            12559..13134
                     /locus_tag="PL014_04815"
     CDS             12559..13134
                     /locus_tag="PL014_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT56445.1"
                     /translation="MRAEEQVEAVVVDDIAERLRDGSREALEEAYARWASLVHTLAVR
                     SLGNHHDAEDVTQQVFVSAWNSRHTLRPDKGTVPGWLVGITRHKVADVHAKRARLARD
                     AQAVAENTLPDESAPPPDDLLATRLVLHDELERLGEPRGTIVRMAFLEDRTHDEIAER
                     LAMPLGTVKSHVRRGLLHLRTRLEEVDRDPS"
     gene            13121..13915
                     /locus_tag="PL014_04820"
     CDS             13121..13915
                     /locus_tag="PL014_04820"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOT56446.1"
                     /translation="MTHPDDDMLAALALGESTPNDVAAHVGGCAPCSREVTALRSTLS
                     VLREPVPALIAPPASVWDAVLAEIDAPETAAAPPTATVAALPAAPTAVDHAVAAPATA
                     QPPTDELATRRENRRRGGIPLAWVAGAAAAGILLGIAGGRLLGGEDPAPAPITVASTS
                     LDTLDTGQVKGSADIVRLDGQLDLAVRTEPIDPRDGYLEVWLINKDLERMVSIGVLRP
                     DAPEQTFAIDQALIDQGYVIVDISREGFDAAPQHSGDSVVRGTLAT"
     gene            13943..15643
                     /gene="ilvD"
                     /locus_tag="PL014_04825"
     CDS             13943..15643
                     /gene="ilvD"
                     /locus_tag="PL014_04825"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOT56447.1"
                     /translation="MTSTPDIKPRSRDVTDGLEKAAARGMLRAVGLGDDDFAKPQIGV
                     ASSWNEITPCNLSLDRLAKAVKEGVHAAGGYPLEFGTISVSDGISMGHEGMHFSLVSR
                     EIIADSVETVMMAERLDGSVLLAGCDKSLPGMLMAAARLDLSSVFLYAGSILPGIAKM
                     SDGSERTVTIIDAFEAVGACARGLMSREDVDAIERAICPGEGACGGMYTANTMASVAE
                     AIGMSLPGSAAPPATDRRRDGFARKSGEAVVELLRRGITARDIMTKEAFENAIAVVMA
                     FGGSTNAVLHLLAIAHEADVDLSLSDFARIGAKVPHLADVKPFGSYVMTDVDRVGGVP
                     VVMRALLDAGLLHGDCLTVTGRTMAENLAHINPPDVDGKVLRAMSEPIHGTGGITILT
                     GSLAPEGAVVKSAGFDSDVFEGTARVFDGERAAMDAVEDGTMTAGDVVVIRYEGPKGG
                     PGMREMLAVTGAIKGAGLGKDVLLLTDGRFSGGTTGLCVGHVAPEAVDEGPIAYVRDG
                     DGIRLDVANGRLDLLVDDDELERRRAEGVTHPEAKYTRGVLAKYRRLVGSASQGAVCD
                     "
     gene            15636..16406
                     /locus_tag="PL014_04830"
     CDS             15636..16406
                     /locus_tag="PL014_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56448.1"
                     /translation="MTDPPGDRPALELAGLVKEFRGRRVVHGLSLAVPRGSLYGLVGP
                     NGAGKTTTLSMATGLLRPDGGTAHVLGHDVWRDPASAKEVMGVAPDGMRLFEQLSGRE
                     LLSYVGALRRMPRDVTSARADELLAVLDLADDASTVVADYSAGMVKKISLAAALIHAP
                     RLLVLDEPFEAVDPVSGQTIRTILRRYVAGGGTVVMSSHVMELVEGLCDRVAVIAGGR
                     VLAEDTVAGLTRGGSLHDRFLELVGASDVGGDELSWLG"
     gene            16417..>16602
                     /locus_tag="PL014_04835"
     CDS             16417..>16602
                     /locus_tag="PL014_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56449.1"
                     /translation="MTWLIVRLRLAMWRTAMSRSTLQLTSSIIGGLAALAVVGTLGPG
                     LVLLVAQPLRITALVVPL"
BASE COUNT         2321 a         6039 c         5840 g         2402 t
ORIGIN      
        1 tgcccatcct cggcaagacg aacatggacg agttcgccat gggctcctcg accgaacaca
       61 gcgcctacgg cccgagccac aacccgtggg acctctcgcg catccccggc ggttctggcg
      121 gtgggtcgag ctcggtcgtc gcctcgttcc aggcaccgct ggcgatcggc accgacaccg
      181 gtggctcgat ccgccagccc gcagccgtca ccggcaccgt cgggaccaag ccgacctacg
      241 gcggggtctc gcgctacggg ctcgtggcgc tcgcgtcctc gctcgaccag gccggcccgt
      301 gcacccgcac ggtgctcgac gccgccctgc tccacgaggt catcggtggg cacgaccccc
      361 tcgactcgac gtcgatcgac gccccggtgc cgcccgtcgt cgaggccgcc cgccgcgccg
      421 acgtcgccgg catgaagatc ggcatcatcc gcgaactgac cggcgacggg ttccaggcgg
      481 gggtgcaggc gcgtttcgac gaggccgtcc agcacctcgt cgacgccggg gcagaggtcg
      541 tcgaggtgtc ctgcccgagc ttcagctacg ccctggccgc gtactacctc atcctcccga
      601 gcgaggcctc gagcaacctc gcgaagttcg acgccatgcg ttacgggctg cgcgtcgccc
      661 ctgacggcat cgacgcaccg agcgccgagc aggtcatggc cgccacccgc gacgccggct
      721 tcggcgacga ggtcaagcgg cgcatcatcc tgggcacgta cgccctgtcc agcggctact
      781 acgacgccta ctacgggtcg gcccagaagg tgcgtcgcct catcgccgac gacttcgcag
      841 cggccttcga gaccgccgac gtcctggtct caccgacggc gccgacgacg gccttccggc
      901 tcggtgacaa gctcgacgac ccgatggcga tgtacctcaa cgacatcgcg accatccccg
      961 ccaacctcgc cggcatcccc ggcatgtcgg tgccgagcgg gctcgcgacc gaggacgggt
     1021 tgccggccgg cttccagatc ctcgcccccg cgatgcagga ccagcggttg tatgccgtcg
     1081 gtgcggccct cgaggccagg ctcaccgccg cgtggggtgg gccgctgctc gaccgggcgc
     1141 ccgacctgct cggtaacgtc cggcaccttg ggccccagga cagccacctt gccggacgca
     1201 acgcaggaac ggaagcctga gccatgaccc agacccacgc cgaggcgacc cttcccttcg
     1261 acgacgcgat cgccgcgttc gaccccgtca tgggcctcga ggtgcacgtc gagctcaaca
     1321 ccgcgacgaa gatgttctgc ggctgcccga ccgagttcgg cgccgagccc aacacccaga
     1381 cctgcccggt gtgcctgggc ctgcccggtg ccctgccggt cgtcaacgcg gtggccgtgg
     1441 agtcggcgat ccgcatcggg ctcgcgctca actgcgagat cgcgcagtgg tgccggttcg
     1501 cccgcaagaa ctacttctac ccggacatgc cgaagaactt ccagacctcg cagtacgacg
     1561 agccgatcgc cttcgagggc tacctcgacg tcgagctcga cgacggcacg gtctaccggg
     1621 tcgagatcga gcgggcccac atggaggagg acaccggcaa gtcgctgcac gtcggcggct
     1681 cgacgggtcg catccacggc gcgacgcaca gcctcgtcga ctacaaccgc gccggcatcc
     1741 cgctcatcga gatcgtcacc aagcccctga ccggggctgg ggagcgggcc cccgagatcg
     1801 cccgcgccta cgtggccacc ctgcgcgacc tgctcaaggc cctcgacgtc tcggacgtgc
     1861 ggatggagca gggctcgatg cgctgcgacg tgaacctctc gctcatgccc aagggcaccg
     1921 aggtgttcgg cacccgctcg gagaccaaga acgtcaactc gctgcgctcg gtcgagcggg
     1981 cggtgcgcta cgaggtgggg cggcacgccg cggtgctcag ctcgggcggc accatcgtgc
     2041 aggagacgcg gcactggcac gaggacaccg gggtcacgac ctcggggcgc gcgaagtccg
     2101 acgccgacga ctaccgctac ttccccgagc ccgacctcgt gccggtggcg ccctctcgtg
     2161 agctggtcga gtcgcttcgg gcgacgttgc ccgagccgcc ggcagcccgg cgcaagcgac
     2221 ttcagggtga gtggggcttt gccgacctcg agatgcgcga cgtcgtcaac gccgggctgg
     2281 tcgacctcgt ggaggagacc gtcgccgcag gtgcgtcccc ggcgggtgcg cgcaagtggt
     2341 ggtccggtga ggtggcgcgg cgcgccaacg ccgagggtgt cgacgtgccg acgtacgccg
     2401 ctgggctggg ggtcggcgcg gcatccgtcg tcgagctcga gggcctcgtg acgtctggtc
     2461 gactcaacga ctcgatggct cgtcaggtct gggagggcgt gctggccggg gagggcactc
     2521 cgacgcaggt cgcggacgcc cgtgggctcg agctggtgca ggacaccggc gccctcgagg
     2581 cggccgtgga cgccgtcatc gcggccaacc ccgacgtggc cgacaagatc cgtgacggca
     2641 aggtcgcggc cgcgggcgcg ctcatcggtc aggtcatgaa ggcgatgaag ggccaggcgg
     2701 atgccggtgc ggcgcgggcg atcatcatgg agcggctcgg ccagtcctga ccccggggcc
     2761 gggcctgcct gcccgacgtc cctgcatcga ccaataggtc gatgcaggga cgtcgccggc
     2821 tgttcgtgag gtgacctatt ggtcgatgtg gggtccgttc cgcggtgcgg aaccccgctg
     2881 cgtggacccc acgggggctc tgatagtctg gaatcactcc agttcggccg gcggcaatgg
     2941 tgacgccccc tggaggaacc ccaccaccct cacaaggaga agtgacgtcg tcatgtccct
     3001 cgtcaacacg accatcaagc ctttcgaggc caccgccttc gtcaacggct cgttcgttcc
     3061 ggtgtcctcg gcggacatcg aggggaagtg ggcgatcttc ttcttctacc cggccgactt
     3121 caccttcgtc tgcccgaccg agctcggcga cctcgccgac cactacgagg agctcaaggc
     3181 cctcggcgtc gaggtctact cggtgtccac cgacacgcac ttcacccaca aggcgtggca
     3241 cgaggcgtcc gacaccatcc gcaaggtcac gtacccgatg atcggcgacc cgacacagcg
     3301 catctccaag aacttcaaga tcctgcgcaa ggccgagggc ctggccgacc gtggcacctt
     3361 cctcgtcgac cccgacggtg tgatccagtt ccacgaggtc accgccgagg gcatcggccg
     3421 caacgccgcc gagctcgtgc gcaaggtcaa ggccgcccag tacgtccgca accacccggg
     3481 tgaggtctgc cccgccaagt gggaagaggg cgaggacacc ctggccccgt cgctcgacct
     3541 cgtcggaaag atctgatcgc atgctcgatc cggctctcgc caagcagctg tcggcctacc
     3601 tgaccaaggt ggtcgacccc gtcgaactcg tcagcagcct cgacgagtcg gccacctcgg
     3661 gccagctcgc cgacctcctc gacgagatcg cgagcctgtc cgccggccgg atcacccacg
     3721 tgcgcggtgg ggatgccgcg cgccgcccct cgttccgggt cgtgcgcgtc ggcaccgccg
     3781 tcgaggtgga gttcgccggg atgccgctcg ggcacgagtt cacctcgctc gtgctcgcgc
     3841 tgcttcaggt gggcgggcac cccgcaaccg ctgccacgga actcctggat gccgttcgcg
     3901 gcctcacggg ggagcaccgg ttcgagacgt acttcagcca gtcgtgccag aactgcccgg
     3961 acgtcgtgca ggcgctcaac ctgatgagca tcctcaaccc cggcatcagc cacgtcgcca
     4021 tcgacggcgc ggtcttcccg ggtgaggtcg aggagcgcgg cgtgctcgcg gtgcccatgg
     4081 tctttcgcaa cggcgagctc ttcggccagg ggcggatgac cctcgaggag atcgtggcgc
     4141 gtctggatga gggcgccgca gctcgcgacg cccagcggct cgatgagctg gagccgttcg
     4201 aggtgctcgt cgtcggtggt gggcccgcgg ggtcgaccgc ggcgatctac accgcacgca
     4261 agggcatccg caccgggctg ctcgccgagc ggttcggtgg gcaactgctc gacacgatgg
     4321 cgatcgagaa ctacccgtcg gtggagcaca cggaaggtcc gcggatggcc gccgacctcg
     4381 agcgtcaggt ccgtggactc ggcgtcgagg tcatgaccgg gcaggtcgcc gtgcggctcg
     4441 tcccggcatc gcgggagccg caggggtcgg ggctgcacga ggtgcacctg gcctcgggtg
     4501 cggtgctgcg ggcccgcgcg gtcgtggcgg cgagcggggc ccggtggcgc accctcgaca
     4561 tccccggtga ggacgagtac cgcaaccgcg gtgtcacgtt ctgcccgcac tgcgacgggc
     4621 cgctgttcgc tgggaagcgg gtcgccgtca tcggcggagg caactcgggg gtggaggcgg
     4681 ccatcgacct cgcgggtgtg gtcgcccacg tcaccctcgt ggagttcgcc gacgagctga
     4741 aggccgacga cgtcctccag cgcaaggtgc actcgctggg caacgtcgac gtcctggtcg
     4801 gcactgccac gaccgaggtc gtcggtgacg gctcgaaggt cgtcggtctt cggcacgccc
     4861 cgcgtgctgg cggagcgtcg agcgtgctcg acgtcgacgg ggtcttcgtc cagatcggcc
     4921 tgctgccgac gaccgagtgg ctcgacgggg tcgtcgacct gacaccccac ggtgaggtgg
     4981 tcgtcgacgc acggggagcg accggcatcc ccggggtctt cgccgctggt gactgcacga
     5041 ccgcgccgtg gaagcagatc gtcgctgccg ccggctcggg tgctggcgcc gcgctgtcgg
     5101 cgttcgacca cctcatccgc accacgtcgc cagcatcgag cccgtcggcc tgacgcggct
     5161 caccgatcag cggcggccgc cgcccagcat cccgccgagg atgtcgccga tgatgctgcc
     5221 ggcgtccagg ccaccggggg cagccgcgcc accacccgat ggggccgtcg cggctccgcc
     5281 cgtcgcacca ccgaggacgt cacgcagcat gtcgtcgagg gagcccgggc cgccggccgc
     5341 gccacccgtg gagcccggca ggctgggggc ggtgtcggtc cgctgcggag cggcaccgcc
     5401 tccgcccagg cccccaccgc cgccgccagc accgcccagc acctgcttgg ccagccagga
     5461 caaaaccatc ggcgcgagga tgggcagcag cttcttgacg agcgccccgc ccatgcccga
     5521 gccgcccatc gcgggcacct cgccgagccg gttgacgacg tcctcctcgc ggtcgccgaa
     5581 gatgtggttg gtgatggcct gaccgtcgct ctcgttgatc gcggagaggt cggcaccgcc
     5641 gctcagcagg tcgtcctggt gctggccgag ggcctcgagg atcgagccgg cacccgaggg
     5701 gtcgctggcg ttggcctgca gaccacccag cagtgccggc agcacggcgg ccgctgcggc
     5761 gcgaacttcg tcgggggagg cgccgacctg ctgggagatg gcgtcggccg gcagcgcgcc
     5821 gaggatgtcg tcgagtgcgg acattgagat cccttctccg gcggcggaac ggctccgccc
     5881 gttgcgcccc aacctagcct caggtcgcga cggtcgggag ggcattgagc gacagggcgc
     5941 ccctcgtggg tgcatgatct ggggcatgac ccgtgcagcg caggaccgaa ccctcgtcct
     6001 ccttcgccac gcgaaggccg agcagcagaa ctgggacaac gaccacgaac gcgagctcac
     6061 cgctcgcggt cgtcgagatg ccgcggccgc gggccggtgg ctcatcgagc acggcatcgg
     6121 gatcgacgag gtgttgtgct cgacctccga gcgcacccag cagaccaccg aggcggtgtg
     6181 gtccagcggc tgccccgagg gtgaggtgca cctcgaccgg cgcatctacc tcgcgtcacc
     6241 ggagcggctc ctcgaggtga tccacgaggc cgacgaggac gccgacgtgc tcatggtcgt
     6301 cggccacgca cccggcatcc cgtccctggc cgagctgctc tgcgacggtg agggcagtgt
     6361 cgagggtcac cgcaagatgg ccgagggctt ccccacctgc gcagttgccg tcctgcacta
     6421 cgcgggtccg tgggccgggc ttcagttcgg cgatgccacc ctcgagcgct tccacgtctg
     6481 ccgcgacgac gactgacccc tgaccccttg tgccgatcga gaggtgtgcg gtgagcatgt
     6541 ggcgaacacg atgggacgag atgtcgccga cgtcgcgggc cctggtgacc acggtggccg
     6601 tcgtcgacgc aggccttcgg gcgtgggcac tggtcgacct gaggaaccgg cccgctgacc
     6661 aggtcaaggg gcccaaggcc gtctgggggc tggcgctgtc cgtcgtcagc tcggccgggg
     6721 tcctgccggc ggcctacctc gtgctcggcc gccgtccccg cggctgagct agccgaccgc
     6781 tgcagcctcc tcggccgcgt cgtcctcgac tgtcacgtcg tcgccccagc gccgcagcac
     6841 ctcgatcgcg gtcagtgcca aggccaccag gacggcgatg acgagcacgc cgcgggcact
     6901 gggtcggtct ccgacgagga tggccaggac cgccaacccc cctactgccg cccgcaggcc
     6961 ggagacgttg tgggcggcga agcgggcgcc ggtggccagc gcaccggtcg gttgccgggt
     7021 cgacccggcg agtcggctcg gcagggtgcg tgcggcgcgc gcggcgctcg acggcccggc
     7081 cagccaggcc ccgaccgcga tgagcaggaa cagcactgcc gaggctcgca cccacaggcg
     7141 caggggcatc gcgacggcgt cgaagagcac ctcgccggcc tcggggacga gtagcgcctg
     7201 ctcgaggtcg ccgaggtaga gggcccgcag cacctgcacc cccagcagga gcatcacgag
     7261 tgagcccacc gccatcgatg acccgacgac cagcgccctt cggcgggacg gggccagcgc
     7321 caccccggcc acgagcagca gcaccgcgct caccggcagc acggtgtgga gcaggtccag
     7381 cagccggacg gccgtctgtg cctggccgag attcgcgctg tcgagcacga cgaactgggc
     7441 gttcacggtg gggatgcggt ccgcgaacgg cacgccgcgc tcgacgagca cgggcttgat
     7501 cgcctcgatg aaggccgcca ggttgaccga gacccgcccg tccgtgacgg acacgccagc
     7561 gctggtctgc ccagtgaggg cggcgacgag gtcagcgtgg gcctctcggt tcgcggcgat
     7621 ccaggcgtcc tggaacccgt cggacgccac gagccgcgag acctgttcgg aggtgaaccc
     7681 gcgcacggcg ttggagatgg ggacggcgaa cgacgtgatg tccaggggca gggtgtcggt
     7741 gacaccctcg agccggggtc gctccgcgat ggctgccagg gtcgtggcgg tgcgctcctc
     7801 gacctgcagt gcctcgaaca cggcatcggt gatccggcgg gagatctcgt cctggatcgc
     7861 cgggtcctgc gcgagtggcg ccaccatggc gacgtactca tccgtgtcga gcagggtcgc
     7921 gttcgaccac gtcgccaggg ccgacagggg ggcgatcagg gcggcgagga gcatcaggac
     7981 gatcgacccg gcccaccggc cacgggtgga gcgcggcccg cccccggccg taccggcagg
     8041 ggtggccggg tccgtcggat cggcggtggc ctgcgcggcg accagcgcgc gcagccgctc
     8101 gagctctgcg cgctcgtcgg tggacagggg ctgggtcact gcggcaccgg tgggtcggct
     8161 catggtcatc cttcgtcgcg cccgcggcgg cggggccgca gccgggccgg ccggcgaccg
     8221 tgccgcgtca cccctcgaat ctagggacgg cgggcgcggc cctcttcacc ccgctcgggt
     8281 gaccctcccg tgcccgtcgc ctcctggacc cactcccaga aacggccccg ctgctcagcc
     8341 gtgggtcgcg tgcggcgcat gatgctcgtc ggccggggtc gacggtcgtg ccacagctgg
     8401 ccggatgccg gtgccgggtc ggccgcgacg acccacgtcg tcgtgtcggc accttcaccc
     8461 gccgtgcgca gcgacgggcc cagcagtgcg ttgaaccggg ggagtgaggt ctgcacgccg
     8521 ggagtcgccg cccaccccgg gtgcgtcgcg ctgacgctga ctcccgcggg gagccagcgt
     8581 agtgccagta cggggagcag ctcgatctgg ccgcgcttgc tgcgcgcgta ggccgtggtg
     8641 ccggagtact cgccacgcag gaactcgggg tcgtccacgg gcaggggctg gctgtacatg
     8701 ccgccggagg tcacgaacac cacccggccc gcatcggccc cgagcagggg cagcagcagc
     8761 tcggtcatga gcaccggccc gagcacgtgc agggccatcg tcagctcgtg gccctgtggg
     8821 gactcagtgc gggtggcggg cagcgccccg gcgttgtgca cgagcgctcg caacggcaac
     8881 ccggcagcga ggaaggccga gcagaaggtg cgcaccgagt cgaggtcggc gacgtcgcac
     8941 acccagaccc gggccgcgcc ggccctgccg accgccgact cgatctcggc ggccacccga
     9001 ccaccgcggc cggcatcccg gacgacgagg tgaacggtgg cacccagtgc tgcgaggtca
     9061 tgggcggtgc gttcgcccag gccggacgat gcgccggtca cggccacgtg gtgcccggtg
     9121 aggatgccgg gggtcgggtc ggcgggccag gtcggcaacc gtctccgcac ggcgtacccg
     9181 agccgggtga atcccggggc gatggtgcgg tcgagcgtgc tgtccagggc gcgggccgct
     9241 gccgtcgtca gccctgtcac atcagcgcct tcatcgcggt gaccgcgccc cgcaggtacc
     9301 gctccacgac cacgcgctcg tcgtcggtca gggaggcgtc gatctcggcg agccctcgga
     9361 acatcggggc gagccgggtg aggacctcga cgcgacccga gtcggtgatg acgacctcgg
     9421 tgcgtcggcc gtcgtccggg tggggtcggc gctgggcgtg gccgcgcccg acgaggcgat
     9481 cgacgatccc cgaggatgcc gcggaggtca ccccgagttc acgtgccagc tcgaccggac
     9541 ccatgggggt ggccatcagg tggcgcagcg agtgcagttc ggagaccgac agaccagcct
     9601 ggcgggccac ctcgtggggc acctgcccgg cgacgtcgac gagttcctgg agggcgacga
     9661 gggaaggtga ctgccgataa cccggcacca ccgacgtgac gtcgggagca acgttagaca
     9721 aacttgcatc aagggcttgg ggctccgtaa tctcactcac ctacttagca acatggtgaa
     9781 tcacctactg aatcagcacg gtatcaaggg agagcggaca catgggcaac cttgcacacg
     9841 ccatcacgcg caagtgggtg gccggcatcc tcgcgctggt ggccattctc ggcgccgggg
     9901 cgatcatcgg tgtggtcggg caggccgagg ggccgcccac cgcggtggcc gcgttgccgg
     9961 acggcaccga cagcaaggcc gccgccgagc ttcgagccga gctgcccgag gcagaggggt
    10021 ccgcggccgt cgtcctctac agcagcgacg cagccctgac ccctgatcag ctggccgtcg
    10081 tccaggaacg ggcacgcacg ctgccggggg cgaccggtgc tccccccgcc gtggcggagg
    10141 acggaactgc cgccacggtg ttcatccccg tgaacacgag tgacgcagtc gagaccgccg
    10201 aggtcgtcgg cgacctgcgg gaggcagcca aggcagacct cccggacggc ctgacagccc
    10261 aggtcaccgg ccccgccgcc atccaggccg acctcgcggc cgtcttcgac ggggcgaact
    10321 tccgcctgct cgccgcgacg gcatccgtcg tcgccctcct gctcgtcatc acctaccgca
    10381 gcccggtcct gtggctcgtg ccgctcactg tcgtcggtgt cgccgaccag ctcgccgcgg
    10441 tggcggcgac ccacaccctg gcggccttcg acgtcctgtg ggacgagtcg acgatcggca
    10501 tcctctccgt cctcgtgttc ggtgcgggca ccgactacgc cctgctgctc atcagccggt
    10561 accgcgacca gctgcgggtc accgaggacc gtcgtgaggc catggcgatc gccctgcgcc
    10621 gcaccaccga ggcgatcgtc gccagctcca gcacggtcgt catcggcctg ctcaccctgc
    10681 tcctctcgct cttccccgcg acccgggggc tcggcctggc ctgtgccgtc ggtgtcgtcg
    10741 tcgcggcctt cttcgtgctc gtcgtgctcc ccgcagccct ggtcgtgttc ggtcgctgga
    10801 tcttctggcc caaggtcccg aaggtgggcc aggccggtct cgccgacgcc aactcgctgt
    10861 ggcgccgcat cggtgacgcc gtcgcgaagc ggcccgccgg tttcgtcacc gtcacgctgc
    10921 tcgcgctggc cgtcatggcc ggtggcatca cccagatcaa gaccggcctc gacggccccg
    10981 accagttcct gcagaagccc gaggccatcg ccgcggccga gcgcctcgga gagtcgttcc
    11041 ccgcaggtgc tgccgacccg accatcgtgg tgacccgcgg tgacggggag gccacggcca
    11101 cggccgtcgc cgccgtcgac ggcatcagcg acgcccgggt cgtcgcctcc ggcaacgggg
    11161 tgagcgagat cgacgcggtc atcgacgccg cccccgggtc cgacgaggcc gaggccacgg
    11221 tcacggcact gcgtgaggcc ctggccggtc aggcggacac ccacgtcggt ggcaccgagg
    11281 ccgaggccat ggacgcctcg gcatccgcgg cccgagaccg tctggtgatc ttcccgatca
    11341 tcctcgcgct cgtgctcgtc tcgctcgctg cgctgctgcg ctcggtcgtg gcgccgatca
    11401 tcctcgtcgc cacggtggtc gcgacctaca tggccagcct cggcctgtcg tgggtgctgt
    11461 tcacccaggt gttcggcttc gagcgactcg acgacggtgt gccgttgctc gcgttcgtct
    11521 tcctcgtcgc cctcggcgtg gactacaaca tcttcctcat cacccgagcc gccgaggagg
    11581 ccaaggacca cggctcacgc agcggcatgt tgcgcgccct cgcggcgacc ggtggtgtca
    11641 tcaccagcgc cggcatcctg ttggccgcgg tgttcgcggt cctgggtgtg ctgccgctcg
    11701 tcgtgctcgc ccagctcggc gtcatcatct gcgtgggcgt gctcctcgac accctcgtgg
    11761 tgcgcaccgt gctcgtcccg gcgatcgggc tcatcctcgg tgaccgcttc tggtggccgc
    11821 ggcgcgtcga cacccgcccc tcggtcgcgg atgccgcgga ctcgactgat cgtgctgaag
    11881 gcccggtgcc cgtgggtggg gcgtgaccct cacccggttc ggcctcgggg tcaccctggc
    11941 cgcactgatc ggcctgtacg ccgtcctcgc ggtgcggtgg accggagccg ggtccgcctg
    12001 ggctaccacg ctggttcgtc cggcgtggca gccgccggac gtggtgttcg gggtggtctg
    12061 gccgctgaac ttcatcgcga tggcggtggt cggcacggcc gtcaccgtcg gggccacccc
    12121 gcgcgaggga gtgctctgga tggtgctgtt cggcgccagc gtggcgctgg cgctcgggtg
    12181 ggcgagggcc ttctacctcc ggcaccggct gggtcggtcc gccgcgctgc tcagcctggc
    12241 tgccgcggtc acctgggccc tcgtggcgct cacggcgagc acggtcggct ggccggcttg
    12301 ggtgctcggg ccgtacgcgg tgtggctcac ggtcgcctcg agcctcgcgg tggggtactg
    12361 gcggttgaac gaatctcggt gacggtgcat ccacagggcc ggtcggcagc gaaccactgt
    12421 cgagacagcc atgaccccgt gagaagccga gaggagtgca gcatgaccca gctcaccatg
    12481 gatccgccgc gctttccatg gcaggcggtg cgtgcgccgg gtcggcctga ccggccgatc
    12541 ccgtttatcg tggggtccgt gcgagcagag gagcaggtcg aggcggtcgt cgtggacgac
    12601 atcgccgaac ggctgcgcga cgggtctcgg gaggcgctgg aggaggccta cgctcgctgg
    12661 gcgtccctcg tccacaccct ggccgtgcgc tccctcggca accaccacga cgcggaggac
    12721 gtcacccagc aggtgttcgt ctccgcgtgg aacagccggc acaccctgcg gcccgacaag
    12781 ggcaccgtgc cggggtggct cgtcggcatc acccgccaca aggtcgccga cgtccacgcc
    12841 aagcgcgctc ggctggcgcg cgacgcccag gcggtcgcgg agaacaccct gcccgacgag
    12901 agcgccccac cgccggacga cctgctcgcg acccggctcg tgctgcacga cgagctcgag
    12961 cgcctcgggg agccccgggg gacgatcgtg cgcatggcct ttctcgagga ccgcacccac
    13021 gacgagatcg ccgagcgtct cgcaatgccc ctcggcaccg tcaagagcca tgtccgccgc
    13081 ggactgctgc acctgaggac ccgcctggag gaggtggacc gtgacccatc ctgatgacga
    13141 catgctggcc gccctggccc tcggcgagtc gacgccgaac gacgtcgccg cacacgtcgg
    13201 tggctgtgcc ccgtgctcac gtgaggtgac ggcgctgcgc tccaccctga gcgtgctgcg
    13261 cgagcccgtc cctgctctca tcgcgccccc cgccagcgtg tgggatgccg tgctcgccga
    13321 gatcgacgcc cccgagaccg ccgccgcgcc cccgacggcg acagtcgccg ctctgccggc
    13381 cgcccctacg gcggtcgacc acgcggtcgc cgctcccgcg accgcgcagc caccgaccga
    13441 cgagctcgcg acgcgccgcg agaaccgcag gcgaggcggc atccccctcg cgtgggtggc
    13501 cggtgccgcc gccgccggaa tactcctcgg catcgccggt ggtcgcctgc tcgggggcga
    13561 ggacccggcg cccgcgccga tcacggtggc cagcaccagt ctcgacacgc tcgacaccgg
    13621 gcaggtcaag gggtcggccg acatcgttcg cctcgacggc cagctcgacc tcgccgtgcg
    13681 caccgagccg atcgaccccc gcgacgggta cctcgaggtg tggctgatca acaaggacct
    13741 cgagcgcatg gtgtcgatcg gggtgctgcg tccagacgcc cccgagcaga ccttcgccat
    13801 cgaccaggcc ctgatcgacc agggctacgt catcgtcgac atctcccgtg agggcttcga
    13861 cgcagccccg caacactcgg gcgacagcgt cgtgcgcgga accctcgcga cctgacccga
    13921 gtgcgctccc taggatgggt gcatgacctc gaccccggac atcaagcccc gtagccgcga
    13981 cgtcaccgac ggcctcgaga aggccgcagc gcgcggcatg ttgcgcgcgg tgggtctcgg
    14041 cgacgacgac ttcgccaagc cgcagatcgg ggtcgcgagc tcgtggaacg agatcacccc
    14101 gtgcaacctc tcgctcgacc gcctggccaa ggcggtcaag gagggggtgc acgccgctgg
    14161 cggctacccg ctcgagttcg gcaccatctc ggtgagcgac ggcatctcga tgggccacga
    14221 gggcatgcac ttctccctcg tcagccgcga gatcatcgcc gactcggtcg agacggtcat
    14281 gatggccgag cgcctcgacg gttcggtgct gctcgccggg tgcgacaagt cgctgcccgg
    14341 catgctcatg gccgccgccc gcctcgacct gtcctcggtg ttcctctacg ccgggtcgat
    14401 cctgcccggc atcgcgaaga tgtccgacgg ctccgagcgc accgtgacga tcatcgacgc
    14461 gttcgaggcc gtcggcgcct gtgcccgggg gctgatgagc cgcgaggacg tcgacgccat
    14521 cgagcgcgcg atctgccccg gtgaaggcgc ctgcggtggc atgtacaccg ccaacacgat
    14581 ggcctccgtg gccgaggcga tcggcatgtc cctgcccggc tcggccgccc cgccggccac
    14641 cgaccgccgc cgtgacgggt tcgcccgcaa gtccggtgag gccgtcgtcg agctgctgcg
    14701 ccgcggcatc accgcccgcg acatcatgac caaggaggcg ttcgagaacg ccatcgccgt
    14761 cgtcatggcc ttcggtggct cgaccaacgc cgtgctccac ctgctcgcga tcgcccacga
    14821 ggccgacgtc gacctctcgc tctccgactt cgcgcggatc ggcgccaagg tgccccacct
    14881 cgccgacgtc aagcccttcg ggtcctacgt catgaccgac gtcgaccggg tcggtggcgt
    14941 gcccgtggtc atgcgcgccc tgctcgacgc gggtctgctg cacggcgact gcctcaccgt
    15001 caccggcagg acgatggccg agaacctcgc ccacatcaac ccgccggacg tcgacggcaa
    15061 ggtgctgcgg gcgatgtccg aacccatcca cggcaccggc ggcatcacca tcctcaccgg
    15121 ctcgctggcc cccgagggtg ccgtcgtgaa gtcggccggc ttcgactccg acgtcttcga
    15181 gggcaccgcg cgcgtcttcg acggtgagcg ggccgccatg gatgccgtcg aggacggcac
    15241 catgacggcc ggcgacgtcg tcgtcatccg ttacgaaggc cccaagggcg gccccgggat
    15301 gcgcgagatg ctcgccgtca ccggcgcgat caagggtgcc ggcctgggca aggacgtcct
    15361 gctcctcacc gacggtcggt tctccggcgg caccaccggc ctgtgcgtcg gccacgtcgc
    15421 ccccgaggcc gtcgacgagg gcccgatcgc gtacgtgcgt gacggcgacg gcatccgact
    15481 cgacgtcgcg aacggtcgcc tcgacctgct cgtcgacgac gacgagctcg agcgccggcg
    15541 tgccgagggc gtgacgcacc ccgaggccaa gtacacccgc ggagtgctcg cgaagtaccg
    15601 gcgcctggtc ggcagcgcca gccagggcgc cgtgtgtgac tgacccgccg ggggaccggc
    15661 cggcgctcga actggccggc ctggtcaagg agttccgggg gcgtcgcgtc gtccacggac
    15721 tctccctcgc ggtgcctcgt ggctcgctct acggcctcgt ggggcccaac ggtgcgggca
    15781 agacgacgac gctgtcgatg gcgaccgggc tgctgcgccc cgacgggggc accgcgcacg
    15841 tcctgggcca cgacgtgtgg cgcgacccgg catccgcgaa ggaggtcatg ggtgtcgcac
    15901 ccgacggcat gcggctgttc gagcagctgt cagggcgcga gctgctgtcc tacgtcggtg
    15961 cgttgcgccg gatgccgcgc gacgtgacct ccgcccgtgc cgacgaactg ctggcggtgc
    16021 tcgacctcgc cgacgacgcg agcaccgtcg tcgccgacta ctcggctggc atggtcaaga
    16081 agatcagcct ggcggcggcg ctgatccacg cgccgcgcct gctcgtgctc gacgagccgt
    16141 tcgaggccgt cgaccccgtg tccggtcaga cgatccgcac gatcctgcgc cgctacgtcg
    16201 ccggtggcgg cacggtcgtc atgtcgagcc acgtcatgga gctcgtcgag ggcctgtgcg
    16261 accgggtcgc cgtcatcgcc ggcgggcgag tgctggccga ggacaccgtc gcaggcctga
    16321 cccggggtgg gtcgctgcac gaccgcttcc tcgagctcgt cggtgcctcc gacgtcggcg
    16381 gagacgaact gtcgtggctg ggctgaggcg gatgccgtga cgtggctcat cgtgcggctg
    16441 cgcctcgcga tgtggcgcac ggcgatgagc cgcagcaccc tgcagctcac gtcctcgatc
    16501 atcggtggcc tcgcggcgct cgccgtcgtc ggcacgctcg ggccgggcct cgtgctgctc
    16561 gtggcccagc cgctgcggat caccgccctc gtcgtccccc tg
//
LOCUS       DAOSOC010000038        16511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3539_length_16511_cov_9.637214, whole genome shotgun sequence.
ACCESSION   DAOSOC010000038 DAOSOC010000000
VERSION     DAOSOC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 16511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16511
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3539_length_16511_cov_9.637214"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..494)
                     /locus_tag="PL014_04840"
     tRNA            complement(421..494)
                     /locus_tag="PL014_04840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(458..460),aa:Pro,seq:ggg)
     gene            complement(575..2047)
                     /gene="der"
                     /locus_tag="PL014_04845"
     CDS             complement(575..2047)
                     /gene="der"
                     /locus_tag="PL014_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT56450.1"
                     /translation="MSTEDPTTATADPEAVERALRVGLEDFELSDEDRALLDRDDDDF
                     EAEAEAGPLPVVAVIGRPNVGKSTLVNRIIGRREAVVEDVPGVTRDRVAYDAEWLGHR
                     FTLVDTGGWAIDARGIHLRVAEQAEIAVDLADVVLFVVDATVGATDDDENCVRLLRRA
                     GKPVVLIANKVDDQRTEADAAALWNLGLGQPWPVSALHGRGSGDALDALLEVLPEFSA
                     MGGAYERGGPRRVALVGRPNVGKSSLLNKLVGHQRVVVDDVAGTTRDPVDELVTLGGR
                     DWRFVDTAGIRRRVHQTRGSDFYASLRTQTALEKAEVAVVLVDASEKISEQDVRVIQQ
                     VIDAGRALVIAYNKWDLTDEERRHFLEREIERDLVQIPWAPRANVSARTGRHMDRLVP
                     ALDVALESWGTRISTGRLNAFLGEVVAGHPHPVRGGKQPRILFATQAGTQPPRFVLFA
                     SGFIEAGYRRFIERRLREEFGFVGTPIEISVRVREKRSRR"
     gene            complement(2078..2764)
                     /locus_tag="PL014_04850"
     CDS             complement(2078..2764)
                     /locus_tag="PL014_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT56451.1"
                     /translation="MDDVSTPGPARARLGHRVGRILFRLLYAARAVDADHVPRDGAVI
                     LAANHTGYLDGPLVVSMAPRPSHFLVLAKTFQGFTGHLLRWSGQIPIEQGRGDRTALA
                     QALAVLARGDVLGIFPEGGRGRGDLASAGKGVAWLALQSGAPVVPTACLGTRRTGELA
                     ASWPRLRSRLVVDFGEPVVLDLPDGLPGRARLDLATEQIRTALVAHVHAASARHGIPL
                     PTDVPPDLVD"
     gene            complement(2775..3464)
                     /gene="cmk"
                     /locus_tag="PL014_04855"
     CDS             complement(2775..3464)
                     /gene="cmk"
                     /locus_tag="PL014_04855"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOT56452.1"
                     /translation="MSAPLTIAIDGPSGSGKSSVSRAVARALGVGFLDTGAMYRALTW
                     WCLERDIDLTDADAVAAAARDLPLAVGTDPDAPTVSVAGTDISQAIRTTRVSTSVSAV
                     ATNLEVRAELQQRQRDLMAQIAQETGGVVAEGRDITTVVAPDASVRVLLTASEEARLR
                     RRSAELHGSTDAAAVEATRDQVVRRDRDDSTVSTFTEAAPGVVLVDTSDLDFDQSVAA
                     VLAVVTAETSA"
     gene            complement(3487..4584)
                     /locus_tag="PL014_04860"
     CDS             complement(3487..4584)
                     /locus_tag="PL014_04860"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOT56453.1"
                     /translation="MTRVHVIGTGLLGTSLGLALRRVGEDVTLEDISPTHAALARDLG
                     AGRLAEGPVEDDAGIDPDLVVVATPPDVTAVVVAGALTRWPTAFVTDLASVKGAVVNG
                     LRDVGTDLTRYCGSHPMAGRERSGAVSGRHDLFDGRAWVLTPTEETDPAAIALVTAVA
                     RSVGAAVSTLAPERHDAAVAAVSHVPQLAASLVAARLEPLDDAAVALAGQGLRDVTRI
                     AASDPGLWTQILAGNAPAVRDVLTALVADLERVVDALDDLSESTDAPGARGALAHAIA
                     AGNAGHARIPGKHGAAPTTYTTVRVLIPDEPGQLGRLFAHIGEIGANVEEFHLDHGLG
                     QAFGVAEIDVVPGAAAGLAEGLTERGWHLHG"
     gene            complement(4581..5495)
                     /locus_tag="PL014_04865"
     CDS             complement(4581..5495)
                     /locus_tag="PL014_04865"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT56454.1"
                     /translation="MTPPNPRGPHRPSKGKPSGQGKPAGKGKATGAGKAKRPTSGTTR
                     PSQPTAVPQWPDRPTVDVHDPDGVRLQKLLAAAGVGSRRVCENLITQGRVEVDGQVVT
                     ELGVRIKATQNVHVDGIRIQLDESRVYLAFNKPLGVVTSMSDDLGRVDIGDFVANRKE
                     RLFHVGRLDADTEGLLILTNDGELAHRLQHPRYGVLKTYLAQIQGQVPRDLGRRLREG
                     VMLEDGPVKVDSFRVVDSAPGKALVEVVLHEGRKHIVRRMLAEAGHPVLTLVRTKVGP
                     IHLGDTKPGKWRNLTSTEVGLLYKAAEL"
     gene            complement(5492..6121)
                     /gene="scpB"
                     /locus_tag="PL014_04870"
     CDS             complement(5492..6121)
                     /gene="scpB"
                     /locus_tag="PL014_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOT56455.1"
                     /translation="MTDEHESADAVAPPPEDQLAFDVADFPGGIHAALEAVLMVVDEP
                     VSVMALASAIEQPAADVEAALRELEDGYAAQQRGFTLRQVGGGWRMYSRHEYAPVVER
                     FVLDGQQARLTQASLETLAVIAYRQPVSRQRVAAVRGVNVDGVIRTLLSRGLIEELGH
                     DEESTAILYGTTSYFLERLGLSDLDDLPALAPYLPEVDLLDEIAEGGRS"
     gene            complement(6114..7028)
                     /locus_tag="PL014_04875"
     CDS             complement(6114..7028)
                     /locus_tag="PL014_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOT56456.1"
                     /translation="MATAPEEVGGAAPTDLTPGGVLTRRAPATTFEVSLDNFTGPFDL
                     LLGLISKHKLDVTEIALARVTDDFIAHIKAERAKPTGWDLSQATEFLLVAATLLDLKA
                     ARLLPQSGPEDEEDLALIEARDLLFARLLQYRAFKDIAHTFAQRMATAGRTQPRTASL
                     EPQFAALLPELIMTVTPEQLAMIAARAMAPKQVPTVGLGHLHAPQVSVREQASIIGAR
                     LRRDRVASFRALVADADSTLVVVARFLALLELFRESSIAFEQAEALGELTVRWTGAEE
                     GDIEVDDEFDDDEDQSLTTTEPAESTDD"
     gene            complement(7033..7869)
                     /locus_tag="PL014_04880"
     CDS             complement(7033..7869)
                     /locus_tag="PL014_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT56457.1"
                     /translation="MGPTGRPLPDFPEPAPLASHGPARIIAMCNQKGGVGKTTTTINL
                     GAALAEVGRRVLLVDFDPQGALSVGLGIPTHELDVTIYNLLTERGHDVRDVIAHTKVE
                     GLDVLPANIDLSAAEVQLVGEVAREQILARVLRPVIDEYDVILIDCQPSLGLLTVNAL
                     TAAHGVIIPLECEFFAMRGVALLVETIEKITDRLNPRLQVDGILATMYDGRTLHSREV
                     VASVVEHFGDRVFHTVISRTVKFPDASLAAEPITTYDSAHKGAEAYRQLARELIARGG
                     AA"
     gene            complement(7979..8890)
                     /gene="xerD"
                     /locus_tag="PL014_04885"
     CDS             complement(7979..8890)
                     /gene="xerD"
                     /locus_tag="PL014_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOT56458.1"
                     /translation="MGRAVDAWLTHLDVERGVSANTLAAYRRDLRRWSTYLEQAGVST
                     PADVTEAHVTEFLTRLREGDEHTTPLAATSAARTLVAVRGLHRFLALEGVLDADPARR
                     VSPPKAPSRLPKAIPVDEVERLLEAASVGDTPAALRDRALLEVLYGTGARISEAVGLD
                     VDDVLGEDGVVRLRGKGGKDRIVPLGSYAAQALSAWLVRGRPALAARGGSTPALFLNT
                     RGARLSRQSAWAVLRSCAERAHLNGHLSPHTLRHSFATHLLEGGADVRVVQELLGHAS
                     VTTTQIYTLVTVQRLREVYAQSHPRAR"
     gene            complement(8929..10044)
                     /gene="ald"
                     /locus_tag="PL014_04890"
     CDS             complement(8929..10044)
                     /gene="ald"
                     /locus_tag="PL014_04890"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOT56459.1"
                     /translation="MKVGIPSEVKNNEFRVGITPVGVHELVRRGHEVLIEKGAGLGST
                     ITDDEYVSQGARILDDADDVWGEADMVMKVKEPVAQEYHRLREGLTLFTYLHLAADEP
                     LTRELVDKKVTSIAYETVQLPSGLLPLLYPMSEVAGCLAPQVGAYSMMKAQGGRGVLM
                     GGIGGVANSKVVVIGAGVAGQNAANIALGMGADVTLLDTDLDKLRMSFWRYDNRVAQI
                     ASSSMSIKEQVLGADLVIGTVLIPGAKAPKLVTDEMVAQMKPGSVLVDVAIDQGGCFE
                     HSRPTTHADPTFPVHNSIYYCVANMPGAVPHTSTWALTNATLSYATKLADRGWKDALR
                     ADPALALGLNTDAGHITYGAVAEAFGMDSIGIDEALS"
     gene            10172..10786
                     /locus_tag="PL014_04895"
     CDS             10172..10786
                     /locus_tag="PL014_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56460.1"
                     /translation="MKLYSDIPARRLVQVASDVGIVLWVTLWVRVAGRVHDTTMELAQ
                     PGRDLAGAGSSFRGTMTSAGDGVDNLPLLGDRVATPFRSASGVGTEIEQAGTALVSAV
                     EQLSLLLALTTALVPILIVGLFWFALRLRFARRASAAQRFIDAAPDLDLFALRAMAKQ
                     PMPRLAAVSDDPAGAWRRGDDEIIRALALLELREVGLRPPVVSG"
     gene            complement(10776..12113)
                     /gene="hisS"
                     /locus_tag="PL014_04900"
     CDS             complement(10776..12113)
                     /gene="hisS"
                     /locus_tag="PL014_04900"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT56461.1"
                     /translation="MSTPTKVTPISGFPEFLPAERLLELHFLDVIREVFELHGFAPLE
                     TRAVEPVERLLGKGGDADKEIYGVTRLAGETSVKDAAVGLHFDLTVPFARYVLENAGK
                     LAFPFRRYQIQKVWRGERPQEGRFREFTQADIDVVDVGELAPHFEAEMPLVIAEAFAR
                     LPIGQFRIQVNNRKIPEGFYLGIGLTDVARTLRIVDKLDKIGPQKVTALLIEAGATPE
                     QAEHALALAAISSTDLSFVDAVRALGVEHPTLDEGLDALAAVITAGRAQAPGVLVADL
                     KIARGLDYYTGTVYETQLVGSESWGSVCSGGRYDSLASDGRTTYPGVGISIGLTRLVH
                     LLVTRRGLTATRSTPAAVLVAVNDEDSRDASRDVAAALRARGIPCEIAPSAARFGKQI
                     RYADRRGIPFVWFPGAAGASDQVKDIRSGEQVEADAASWQCPEADLRPGVITR"
     gene            complement(12110..12811)
                     /locus_tag="PL014_04905"
     CDS             complement(12110..12811)
                     /locus_tag="PL014_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT56462.1"
                     /translation="MLAVAFPAAAFATNCYVLATGPGQECLVVDPGIGVEDTLRDVLA
                     EHRLRPAAVLLTHGHLDHVYSVTPVCGGDTAAYIHCDDRYRLTDPIASLAPGTLAMME
                     QQFGQRATWREPETVVEVTDRQTLTLAGLEVEVLHAPGHTEGSVMFGLGVVPDGLPAD
                     EGLDRTMVSGDVLFAGSIGRTDLPGGDHAAMQRSLRDVVLPLPDSTLVLTGHGPATTM
                     RRERGTNPYLQGLTA"
     gene            12922..13722
                     /locus_tag="PL014_04910"
     CDS             12922..13722
                     /locus_tag="PL014_04910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT56463.1"
                     /translation="MTKEQERARARRRHERQLKAIAQREADGARRNRVLAIIATVVAV
                     LGALALLGTVFGSQDPDPSPSAAATTLAGCQPPPEILGTGAELDLPDAATAKGKTYVA
                     TIATNCGDIELTLDGEKAPQAVASFVQLAERDYWRSAPCHRLTTSPTLKVLQCGDPTG
                     TGQGNPGYGFGVENAPADGSYPRGTVAMARTQDPEQGNGGQFFIVYGDSSLPDPNGYT
                     VFGTVTAGLDIVDKIVAAGVGPGGSSPDDGFPAAPISILSVAVTEKKA"
     gene            13725..15086
                     /locus_tag="PL014_04915"
     CDS             13725..15086
                     /locus_tag="PL014_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOT56464.1"
                     /translation="MSDDQPTPRPAIPSPAALASRLQARPVAAPAAPVSHSESARFGR
                     VDDTGTVFVTVGEEEREVGSYPGASAEEALQYFARKYDELAASADLLEARLRNPEVAS
                     KEVADGLTTLKEHIASIGVVGDLAALGATVARVEEGLAAKRAEEAQVRAAARAVAAEQ
                     REALVAEAEKIAAQPAGSTQWKTSGERMRALLDEWKQHQRSGPKLDKTTESALWTRFS
                     HARNTFDKGRRAWFADLETSRADARSTKEALVTEAEKLATSTDWAPTARAFKQLMDRW
                     RTAGRASRAEDDALWERFRSAQDAFFTAKDEVVAAEDESFRANLVVKEALLVEAEALL
                     PVSDVEAAKAALRGIQDRWEAAGKVPRADLERVEKRMRRVEATIREADEQRWKRTNPE
                     VAARARSMVDQLEASVAATQADLAKAEAKGDTKAAAAATSKLAAQQQWLDQARAGLDE
                     FSG"
     gene            complement(15138..>16511)
                     /locus_tag="PL014_04920"
     CDS             complement(15138..>16511)
                     /locus_tag="PL014_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOT56465.1"
                     /translation="RFKDYIAMPKFNMYQSLHTTVIGPGGKPVEIQIRTHTMHRRAEY
                     GVAAHWKYKEEPNAKGAAARGAQGGAQARDGQTGPINDMAWLRQLLDWQKETSDPGEF
                     LESLRFEITASEVYVFTPKGQLVGLPAGSTPVDFAYAVHTEVGHHCIGARVNARLVPL
                     ESRLDNGDVVEVLTSKADSAGPSRDWLNFVRSARARNKIKHWFTKERREEMIDEGKDA
                     LAKVMRKQGLPLQRLTSVESLTGVAEELRLAGIDGLYAAIGEGHVSAQHVVSRLVASV
                     GGEEGASEDIAEAASPTARRTHHRRSGDPGVVVKGMSDVWVKLARCCTPVPGDDILGF
                     VTRGTGVSVHRRDCTNADSLLSQPEKIIEVEWAPTSGSLFLVQLQVDALDRSRLLSDV
                     TRVISDNGVNILSAAVTTSRDRMAKLHFTFEMGDPAHLGHVVRAVQSVDGVFDAHRVT
                     GGKRSDH"
BASE COUNT         2342 a         5737 c         6050 g         2382 t
ORIGIN      
        1 cgtaggagcg ccggtgacca cgcggatcac ccacatatca agcatgagcg cgaaccctgc
       61 gagcgctcgt atccgccagt gcgccaagct tcggtcagcc ccggtccaca atcgttgact
      121 cgcggcggcg gcactgaccg ccacaggccc agccggcacg aactcgtcca aggctgccgc
      181 tgcgtatctc aggtgatgag accagagggt cacacggaca gctatccgat ggcggctaac
      241 gaggccgtcc ttcgcgtttc cccagttcag aaggggtggc ccgtcccctc ggctggacag
      301 ccaccctgag gcggtcccgg cctcgccctc gacgacgtcc aacggttccc ttgttgcacg
      361 tatgttgcac gaaaccggat ttcgtgaccc ccctaggtct ctgacctgca tcaacatcag
      421 tcgggctgac aggatttgaa cctgcgaccc cttgaccccc agtcaagtgc gctaccaagc
      481 tgcgccacag cccgtggctt gccccctcgc gggagcgtcg aaaccatatc ccatggcggc
      541 cggggcgacc caatcgcccc ggcgcgcccc ggggtcagcg gcgcgagcgc ttctcgcgca
      601 cccgcaccga gatctcgatc ggcgtgccga cgaagccgaa ctcctcgcgc aggcggcgct
      661 cgatgaagcg ccggtagccg gcctcgatga agcccgacgc gaagaggacg aaccgcggcg
      721 gctgggtgcc cgcctgggtc gcgaagagga tgcggggctg cttgccacca cgcacggggt
      781 gcgggtggcc ggcgacgacc tcaccgagga aggcgttgag tcgcccggtg gagatgcgtg
      841 tgccccagga ctccagcgcc acgtcgagcg ccggcacgag ccggtccatg tgccgaccgg
      901 tgcgggccga gacgttggcc cgcggcgccc aagggatctg cacgaggtcg cgctcgatct
      961 cgcgctcgag gaagtgccgg cgctcctcgt cggtgaggtc ccacttgttg taggcgatga
     1021 ccagggcccg cccggcgtcg atgacctgct ggatgacccg cacgtcctgc tcggagatct
     1081 tctcggaggc gtcgacgagc accaccgcga cctcggcctt ctcgagcgcc gtctgggtgc
     1141 gcagggaggc gtagaagtcg gagccacgcg tctggtggac ccggcggcgg atgccggcgg
     1201 tgtcgacgaa gcgccagtcg cgcccgccga gggtgacgag ctcgtcgacg gggtcgcggg
     1261 tcgtgcccgc gacgtcatcg acgacgaccc gctggtggcc cacgagcttg ttgagcaggg
     1321 aggacttgcc gacgttgggc cggccgacca gggccacccg ccgcgggcca ccgcgctcgt
     1381 aggccccacc catggcggag aactccggca gcacctcgag cagggcgtcg agggcgtcac
     1441 cgctcccccg cccgtgcagt gccgagaccg gccagggctg cccgagaccc aggttccaca
     1501 gggccgcggc gtcggcctcg gtgcgctggt cgtcgacctt gttcgcgatg agcacgacgg
     1561 gcttgccggc gcggcgcagc aggcgcacgc agttctcgtc gtcgtcggtg gcacccacgg
     1621 tggcgtcgac gacgaacagc acgacgtcag cgaggtcgac ggcgatctcg gcctgctcgg
     1681 ccacccgcag gtggatgccg cgggcgtcga tggcccagcc gccggtgtcg acgagggtga
     1741 agcggtggcc gagccactcg gcgtcgtagg cgacgcggtc gcgggtcacg ccgggaacgt
     1801 cctcgacgac ggcctcgcgc ctgccgatga tgcggttgac gagggtggac ttgccgacgt
     1861 tcgggcgccc gatcacggcg acgaccggca gcgggcccgc ttcggcctcg gcctcgaagt
     1921 cgtcgtcgtc acggtcgagc agcgcacggt cctcgtcgct gagctcgaag tcctcgagac
     1981 cgacgcgcag ggctcgctcg acggcttcgg ggtcggcggt cgcggtggtc gggtcctcgg
     2041 tactcatccc accattgtcc cccacgtcag gggtgcatca gtccacgagg tcgggcggca
     2101 cgtccgtcgg cagcgggatg ccgtggcggg cggaggcggc gtgcacgtgc gccacgaggg
     2161 cggtgcggat ctgttcggtc gcgaggtcga ggcgggcccg gccgggcagc ccgtcgggga
     2221 ggtcgaggac gaccggctcc ccgaagtcga cgacgagtcg ggagcgaagg cgtggccagg
     2281 atgccgcgag ctcacccgtg cgtcgcgtcc ccaggcacgc ggtgggcacg acgggagcac
     2341 cggactgcag ggccagccac gccaccccct tgccggcgga cgcgaggtca ccacgcccgc
     2401 gcccaccctc ggggaagatg ccgaggacgt cgcctcgagc gagcacggcg agcgcctggg
     2461 ccagagccgt gcggtcccca cgaccctgct cgatggggat ctggccactc caccgcagca
     2521 ggtgtccggt gaacccctgg aacgtcttcg cgagcacgag aaagtggctc ggacgcggcg
     2581 ccatcgacac gacgaggggg ccgtcgaggt agccggtgtg gttcgcggcg aggatcaccg
     2641 cgccgtcgcg cgggacgtgg tcggcatcca ccgcgcgggc ggcatagagc aggcggaaga
     2701 ggatccgtcc cacccggtgc cccaggcgtg cccttgcggg gccgggagtg ctgacgtcgt
     2761 ccacgagccc tgcgtcaggc agaggtctct gcggtgacga ccgcgagcac ggcagccacg
     2821 ctctggtcga agtccaggtc ggaggtgtcg acgagcacga ccccgggtgc ggcctcggtg
     2881 aacgtcgaga ccgtcgagtc gtcgcggtcg cgccgcacca cctggtcgcg cgtcgcctcg
     2941 accgccgctg cgtcggtgct gccgtgcagc tcggccgagc gccgtcgcag ccgcgcctcc
     3001 tcggatgccg tcagcagcac ccgcacgctc gcgtccgggg ccacgacggt ggtgatgtcg
     3061 cggccctcgg ccaccactcc cccggtctcc tgcgcgatct gagccatgag gtcgcgctgg
     3121 cgctgctgga gctcggcccg cacctcgagg ttggtggcca cggccgacac cgaggtgctg
     3181 acccgcgtcg tgcggatggc ctgcgagatg tccgtgccgg cgacggacac cgtcggggcg
     3241 tccgggtcgg taccgaccgc gagcgggagg tcacgcgcgg cggcggccac ggcatccgca
     3301 tcagtgaggt cgatgtcgcg ctcgaggcac caccacgtca acgcccggta catcgccccc
     3361 gtgtccagga aaccgacgcc gagggcacgc gccacggccc tcgagacgct gctcttgccc
     3421 gagccggagg gtccgtcgat cgcgatcgtc aggggtgcac tcatgggccg gaagcctact
     3481 ggcggctcag ccgtgcaggt gccagccccg ctcggtgagc ccctccgcca ggcccgcagc
     3541 ggcacccggg acgacgtcga tctcggcgac cccgaaggcc tggccgagtc cgtggtcgag
     3601 gtggaactcc tcgacgttgg caccgatctc gccgatgtgg gcgaacaacc gaccgagctg
     3661 gcccggctcg tcggggatga gcacgcgcac cgtcgtgtac gtcgtggggg cggcaccgtg
     3721 cttgccgggg atgcgcgcgt ggcccgcgtt gcccgcggcg atggcgtggg ccagggctcc
     3781 acgtgcaccg ggtgcgtcgg tggactcgct gaggtcgtcg agggcgtcga cgacccgctc
     3841 gagatcggcc accagcgccg tcagcacgtc acggacggcg ggtgcgttgc ccgcgaggat
     3901 ctgggtccac agcccggggt cgctcgccgc gatccgggtg acgtcccgca gcccctgccc
     3961 ggccagggcc accgccgcgt cgtcaagggg ttccagccgc gccgccacga ggctcgccgc
     4021 cagctggggg acgtgcgaga ccgcggcgac ggccgcgtcg tgccgctcgg gggccagcgt
     4081 gctcaccgca gcccccaccg agcgggccac ggccgtgacg agtgcgatcg cagcggggtc
     4141 ggtctcctcg gtcggggtca gcacccatgc tcgcccgtcg aacaggtcgt gacggccgga
     4201 cacggcaccg gagcgctcgc gccccgccat cgggtgcgat ccgcagtagc gggtgaggtc
     4261 ggtgccgacg tcgcgcagcc cgttcaccac ggcgcccttg accgaggcga ggtcggtgac
     4321 gaacgccgtc ggccaacggg tcagcgcccc ggccacgacc acagccgtca cgtccggagg
     4381 tgtcgcgacg acgacgaggt cgggatcgat gccggcatcg tcctcgaccg ggccctcggc
     4441 gagccgtccc gcgccgaggt ctcgcgccag ggccgcgtgg gtggggctga tgtcctcgag
     4501 ggtcacgtcc tcgccgacgc ggcgcagcgc cagcccgagg ctcgtgccga gcagcccggt
     4561 gccgatgacg tggacccgcg tcacagctcc gcagccttgt agagcaggcc cacctcggtg
     4621 ctcgtgaggt tgcgccactt gcccggcttg gtgtcgccga ggtggatggg cccgaccttg
     4681 gtgcgcacca gggtgagcac ggggtggccg gcctcggcga gcatgcgccg cacgatgtgc
     4741 ttgcgcccct cgtgcagcac cacctcgacc agggccttgc cgggtgcgga gtcgacgacg
     4801 cggaacgagt cgaccttgac cgggccgtcc tcgagcatga cgccctcgcg cagtcgccgc
     4861 ccgaggtcgc ggggcacctg gccctggatc tgcgcgaggt aggtcttcag cacgccgtag
     4921 cgcgggtgct ggagccggtg cgccagttcg ccgtcgttgg tgaggatgag caggccctcg
     4981 gtgtcggcgt ccagccggcc gacgtggaag agccgctcct tgcggttggc gacgaagtca
     5041 ccgatgtcga cacggcccag gtcgtcgctc atggaggtga ccaccccgag cggcttgttg
     5101 aacgccaggt acacgcggct ctcgtcgagc tggatgcgga tgccgtcgac gtggacgttc
     5161 tgggtcgcct tgatccgcac cccgagctcg gtgacgacct ggccgtcgac ctcgacccgg
     5221 ccctgggtga tcaggttctc gcagacccgc cgcgagccca cgccggcggc cgcgagcagc
     5281 ttctgcaggc gcaccccgtc ggggtcgtgg acgtcgacgg tgggacgatc gggccactgg
     5341 ggcacggccg tgggctggct ggggcgcgtc gtgccggagg tcgggcgctt ggccttgccc
     5401 gcaccggtcg cctttccctt gccggctggc tttccttgac cagagggctt gcccttgctg
     5461 ggtcggtggg gtccacgggg gttcggcggc gtcatgagcg gcctccttcg gcgatttcgt
     5521 cgagcaggtc gacttcgggc aggtacgggg cgagggcggg aaggtcgtcg aggtcggaga
     5581 ggccgaggcg ctccaggaag tacgacgtcg tgccgtagag gatggccgtc gactcctcgt
     5641 cgtgaccgag ttcctcgatg aggccgcgac tgagcagggt gcggatgacg ccgtcgacgt
     5701 tgaccccgcg cacggcggcc acccgttggc gcgacaccgg ctggcggtag gcgatgacgg
     5761 cgagggtctc gagggaggcc tgggtcaggc gggcctgctg cccgtcgagg acgaatcgct
     5821 cgaccacggg cgcgtactcg tggcggctgt acatccgcca ccctcccccg acctggcgca
     5881 gcgtgaaccc acgctgctgg gccgcgtacc cgtcctccag ctcgcgcagg gcggcctcga
     5941 cgtcagcggc cggctgctcg atcgccgacg ccagcgccat gaccgacacg ggctcgtcga
     6001 cgaccatgag caccgcctcg agggcagcgt ggatcccgcc ggggaagtcg gccacgtcga
     6061 aggccagctg gtcctcggga gggggcgcga cggcatccgc gctctcgtgc tcgtcagtca
     6121 tcggtgctct ccgctggttc tgtggtggtg agtgactggt cctcgtcatc atcgaactcg
     6181 tcgtcgacct cgatgtcgcc ctcctcggcg ccggtccagc gcaccgtgag ctcaccaagc
     6241 gcctcggcct gctcgaacgc gatcgaggac tcccggaaca gctcgagcag ggcgaggaag
     6301 cgcgcgacga cgacgagggt ggagtcggcg tcggcgacca gggcccggaa cgaggccacc
     6361 cggtcgcggc gcaaccgggc gccgatgatg gacgcctgct cgcgcacgct cacctgcggg
     6421 gcgtgcaggt gacccaggcc cacggtcggc acctgcttcg gggccatggc ccgcgccgcg
     6481 atcatcgcga gctgttccgg ggtcacggtc atgatcagct cggggagcag cgcggcgaac
     6541 tgcggttcga gggatgccgt gcgcggctgg gtgcgcccgg cggtcgccat ccgctgcgcg
     6601 aaggtgtggg cgatgtcctt gaaggcccgg tactgcagca ggcgcgcgaa cagcaggtct
     6661 cgcgcctcga tgagggcgag gtcctcctcg tcctccgggc cggactgcgg aagcagccgc
     6721 gccgccttga ggtcgagcag ggtcgcggcg acgaggagga actcggtggc ctgcgacagg
     6781 tcccagcccg tgggtttggc ccgttcggcc ttgatgtggg cgatgaagtc gtcggtgacc
     6841 cgcgccaggg cgatctcggt gacgtcgagc ttgtgcttgc tgatcaggcc cagcagcagg
     6901 tcgaagggcc cggtgaagtt gtccagggag acctcgaagg tcgtcgccgg agcccggcgg
     6961 gtgagcaccc caccgggggt gaggtcggtc ggagcggctc ccccgacctc ctcgggggcc
     7021 gtcgccatcg gttcaggccg ccccgccgcg cgcgatgagc tcgcgggcaa gctggcggta
     7081 ggcctcggca cccttgtgag cggagtcgta cgtcgtgatc ggctcggccg cgagcgaggc
     7141 gtcggggaac ttcacggtcc gggagatcac cgtgtggaag acccggtcac cgaagtgctc
     7201 gacgacgctg gccacgacct cgcgcgagtg cagggtgcga ccgtcgtaca tcgtcgcgag
     7261 gatgccgtcg acctgaagtc gcgggttgag ccggtcggtg atcttctcga tggtctcgac
     7321 gagcagggcg acgccgcgca tcgcgaagaa ctcgcactcg agcgggatga tgacgccgtg
     7381 cgcggccgtc agggcgttga ccgtgagcag cccgagggag ggctggcagt cgatgaggat
     7441 gacgtcgtac tcgtcgatga cggggcgcag cacccgcgcg aggatctgct cacgggcgac
     7501 ctccccgacg agttgcacct cggccgccga caggtcgatg ttcgccggca gcacgtcgag
     7561 gccctcgacc ttggtgtggg cgatgacgtc ccgcacgtcg tggccgcgct cggtgagcag
     7621 gttgtagatc gtgacgtcga gctcgtgggt ggggatgccg aggccgacgg acagggcccc
     7681 ctgcgggtcg aagtcgacga gaagcacccg gcggccgacc tcggccaggg cggcaccgag
     7741 gttgatggtg gtcgtcgtct tgccgacccc acccttctgg ttgcacatcg cgatgatgcg
     7801 cgccggcccg tgggaggcca ggggcgcggg ttccgggaag tcgggaaggg ggcggccggt
     7861 gggccccatg gtgccggggg cctcgctgcg caacgccgtg tcggaatcca actctctact
     7921 ccccggtaca tggtctggtg ctcgacggtc agcatcgcgc tgatcggggc cgaccctatc
     7981 agcgggcgcg gggatgactc tgggcgtaca cctcgcgaag tcgctgcacg gtgaccaacg
     8041 tgtagatctg cgtggtcgtc accgacgcgt ggcccagcag ttcctgcacc acgcgtacgt
     8101 cggcgccacc ctcgagcagg tgggtcgcga acgagtgccg cagggtgtgc ggcgacaggt
     8161 gaccgttcag gtgcgctcgt tcggcgcagg aacgcagcac ggcccacgcg ctctggcgcg
     8221 agaggcgggc accgcgcgtg ttgaggaaga gagcaggggt gctgccacca cgggctgcga
     8281 gcgccggccg gccgcgaacg agccacgccg acagggcctg cgccgcgtag gagccgagcg
     8341 gcacgatgcg gtccttgccg cccttgcccc gcaggcgcac cacgccgtcc tcccccagca
     8401 cgtcgtccac gtcgaggccc accgcctcgc tgatccgggc cccggtgccg tagagcacct
     8461 cgagcagggc ccggtctcga agggctgccg gggtgtcgcc caccgacgcg gcctcgagca
     8521 gtcgctcgac ctcgtcgacc gggatggcct tgggcagcct cgagggagcc ttgggcgggc
     8581 tcacccggcg tgccgggtcg gcgtcgagca ccccctccag cgccaggaag cggtgcaggc
     8641 cgcgcacggc gacgagggtt cgggccgcgg acgtcgccgc gagcggtgtg gtgtgctcgt
     8701 caccctcgcg cagcctcgtc aggaactcgg tgacgtgcgc ctcggtgacg tcggcggggg
     8761 tgctcacgcc ggcctgctcg aggtaggtgc tccagcgtct gaggtcgcgg cggtaggcgg
     8821 ccaacgtgtt ggccgacacc ccgcgctcga cgtccaggtg ggtcagccac gcgtcgacgg
     8881 cccggcccac cgcagtgggt cgggccgtcg gctcggtgga tcccttggtc aggacagggc
     8941 ctcgtcgatg ccgatgctgt ccatgccgaa ggcctcggcg acggcaccgt aggtgatgtg
     9001 cccggcatcc gtgttcaggc cgagggcgag cgcggggtcg gcgcgcaagg cgtccttcca
     9061 gccgcggtcg gcgagcttcg tcgcgtacga gagcgtggcg ttggtgagcg cccacgtcga
     9121 ggtgtgcggc acggcaccgg gcatgttcgc gacgcagtag tagatcgagt tgtgcaccgg
     9181 gaaggtcggg tcggcgtggg tcgtgggacg ggagtgctcg aagcagccac cctggtcgat
     9241 ggcgacgtcg acgagcaccg aaccgggctt catctgggcg accatctcgt cggtgacgag
     9301 cttgggcgcc ttggcacccg ggatgagcac cgtgccgatg acgaggtcgg cccccaggac
     9361 ctgctccttg atcgacatcg acgacgaggc gatctgggcc acccggttgt cgtagcgcca
     9421 gaagctcatc cgcagcttgt cgaggtcggt gtcgagcagg gtgacgtcag cacccatgcc
     9481 gagggcgatg ttggcggcgt tctgcccggc cacaccggca ccgatgacga cgaccttgga
     9541 gttggcgacg ccgccgatgc cgcccatgag cacgccgcgg ccaccctggg ccttcatcat
     9601 cgagtacgcg cccacctggg gggccagaca gccggcgact tccgacatcg ggtacagcag
     9661 cggcagcaga cccgagggga gctggaccgt ctcgtaggcg atcgaggtga ccttcttgtc
     9721 gacgagctcg cgggtcagcg gctcgtccgc ggcgaggtgc aggtaggtga acagggtcag
     9781 gccctcgcgc aggcggtggt actcctgcgc gaccggttcc ttgaccttca tgaccatgtc
     9841 ggcctcgccc cacacgtcat ccgcgtcgtc gaggatgcga gcgccctggg acacgtactc
     9901 gtcgtcggtg atcgtcgaac cgagcccggc ccccttctcg atgagcacct cgtgcccgcg
     9961 gcggacgagc tcgtggacac ccacgggggt gatgccgacg cggaactcgt tgttcttgac
    10021 ttcgctgggg atcccgacct tcacggttat ctggccctgt ctgtctcgac gaaaacccac
    10081 ggcaccgttg ccgtggcggt gcgctgcgtg ggcgcgcggt gcgagtctag acccgcgcca
    10141 cctgcgtgcc cccgccctcg aggagccctt cgtgaagttg tacagcgaca tccctgcccg
    10201 ccgtctcgtg caggtcgcct ccgacgtcgg catcgtgctc tgggtgacgt tgtgggtgcg
    10261 cgtcgccggc cgcgtgcacg acacgacgat ggagctcgcc cagcccgggc gtgacctcgc
    10321 aggggccggg tcgagctttc gcgggacgat gaccagtgcg ggcgacggcg tcgacaacct
    10381 cccgctgctc ggcgaccggg tcgccacgcc gttccgttcg gcatcgggcg tcggcacgga
    10441 gatcgagcag gccggcacgg cactggtcag cgccgtcgaa cagctctccc tgctgctcgc
    10501 cctcacgacc gctctggtgc cgatcctcat cgtcgggctg ttctggttcg ccctgcgctt
    10561 gcgcttcgcg cgccgggcga gcgccgctca gcgcttcatc gacgccgccc ccgacctcga
    10621 cctgttcgcc ctgcgtgcca tggccaagca gccgatgccg cggctggccg ccgtctccga
    10681 cgaccccgcc ggggcgtggc gtcgcggtga cgacgagatc atccgggcgc tggccctgct
    10741 cgagctgcgg gaggtcgggc tccgcccacc cgtggtcagc gggtgatgac gcccgggcgc
    10801 aggtcggcct ccgggcactg ccacgacgcg gcatccgcct cgacctgctc gcctgatcgg
    10861 atgtccttga cctggtcgct agcgccagca gcgccgggga accagacgaa ggggatgccg
    10921 cgacggtcgg cgtagcggat ctgcttgccg aagcgggccg cgctcggagc gatctcgcac
    10981 gggatgccgc gggcccgcag tgccgcggcg acgtcgcgtg acgcgtcgcg tgagtcctcg
    11041 tcgttgaccg cgaccaggac ggcggccggg gtcgaccggg tggccgtgag gccgcggcgg
    11101 gtgacgagca ggtgcacgag ccgggtcagg ccgatcgaga tgccgacgcc ggggtaggtg
    11161 gtgcgcccgt cgctggcgag cgagtcgtag cggccgccgg agcagaccga accccacgac
    11221 tccgacccca cgagctgggt ctcgtagacc gtgccggtgt agtagtcgag cccacgggcg
    11281 atcttcaggt cggcgacgag cacgccgggc gcctgcgccc tgccggccgt gatcacggcg
    11341 gcgagcgcgt ccagcccctc gtccagggtg gggtgctcga cgccgagggc gcgtacggca
    11401 tccacgaacg acaggtcggt gctgctgatg gccgccagcg ccagcgcgtg ctcggcctgc
    11461 tccggggtgg caccggcctc gatgagcagc gccgtgacct tctgcgggcc gatcttgtcg
    11521 agcttgtcga cgatgcgcag cgtcctggcg acgtcggtca gcccgatgcc gaggtagaac
    11581 ccctcgggga tcttgcggtt gttgacctgg atgcggaact gcccgatcgg cagccgcgcg
    11641 aacgcctccg cgatgaccaa cggcatctcg gcctcgaagt gcggcgcgag ctcgccgacg
    11701 tcgacgacgt cgatgtcggc ctgggtgaac tcccggaagc gcccttcctg gggccgctca
    11761 ccacgccaca ccttctggat ctggtagcgc cggaacggga aggcgagctt gccggcgttc
    11821 tccagcacgt agcgcgcgaa gggcacggtg aggtcgaagt gcaggcccac agcggcatcc
    11881 ttgaccgagg tctcccccgc gaggcgggtg accccgtaga tctccttgtc ggcgtcgccg
    11941 cccttgccga gcagacgctc gaccggctcg acggcccggg tctccagcgg cgcgaaaccg
    12001 tgcagctcga agacctcacg gatgacgtcg aggaagtgca gctcgaggag ccgctcggca
    12061 ggcaggaact cggggaaccc ggagatgggg gtgaccttgg tgggagtgct cacgcggtca
    12121 atccttgcag gtacgggttg gtgccccgct cgcggcgcat cgtcgtggcc gggccgtggc
    12181 cggtgaggac gagggtggag tcgggcagcg gcaggacgac gtcgcgcagg ctgcgctgca
    12241 tggcggcgtg gtcgcctccg ggcaggtcag tgcgcccgat cgagccggcg aacaggacgt
    12301 cccccgacac catggtgcgg tccaggccct cgtcggcggg caggccgtcg ggaacgactc
    12361 cgagaccgaa catcaccgaa ccctcggtgt gcccgggcgc gtgcagcacc tcgacctcga
    12421 ggccggccag ggtgagggtc tggcggtcgg tcacctcgac gacggtctcg ggctcacgcc
    12481 acgtcgctcg ctgaccgaac tgctgctcca tcatcgcgag ggttccgggg gccaggctcg
    12541 cgatcgggtc ggtgagccgg taccggtcgt cgcagtggat gtaggccgcc gtgtctcccc
    12601 cacacaccgg cgtcaccgag tagacgtgat cgaggtggcc gtgggtgagc agcaccgccg
    12661 cggggcgcag ccggtgttcg gcgagcacgt cgcgcagggt gtcctcgacc ccgatccccg
    12721 ggtcgacgac gaggcactcc tgccccgggc cggtcgcgag gacgtagcag ttggtggcga
    12781 aggcggcagc ggggaaggcg acggcgagca cggtgcgagc gtagcccgcc gcatcctgcc
    12841 tagactgggc cgccgatacc ggctccgtcg ccggatgccg gaaccatgcc gatgccggca
    12901 accaggaagg gaccgcgagc catgaccaag gagcaggagc gcgctcgggc gcgccgccgc
    12961 cacgagcgcc agctcaaggc catcgcccag cgcgaggccg acggtgcgcg gcgcaaccgc
    13021 gtgctcgcca tcatcgccac ggtcgtcgcc gtactcggcg cgttggctct cctcggcacc
    13081 gtcttcggct cgcaggatcc ggacccctcg cccagcgccg ccgccaccac gcttgcgggc
    13141 tgccagccgc cgccggagat cctcggcacc ggtgccgagc tcgacctccc ggatgccgcg
    13201 acggccaagg gcaagacgta cgtcgccacc atcgcgacca actgcggcga catcgagctc
    13261 accctggacg gtgagaaggc tccccaggcc gtggcgtcgt tcgtccagct cgccgagcgc
    13321 gactactggc gcagcgcgcc gtgccaccgc ctcacgacgt cgccgacgct gaaggtgctc
    13381 cagtgcggcg acccgaccgg caccggtcag ggcaaccccg gctacggctt cggcgtcgag
    13441 aacgcgccgg ctgacggcag ctacccgcga ggaacggtcg ccatggcccg cacccaggat
    13501 cccgagcagg gcaacggcgg tcagttcttc atcgtgtacg gcgactcgtc actaccggac
    13561 cccaacgggt acaccgtgtt cggcacggtc accgcagggt tggatattgt cgacaagatt
    13621 gttgccgccg gggttggccc cggcggttcg agccccgatg acggcttccc cgccgcaccg
    13681 atcagcatcc tcagcgttgc tgtcaccgag aagaaggcct gaccgtgtcc gacgaccagc
    13741 ccaccccccg ccccgccatc ccctcgcccg cggccctggc ctcccggctc caggcccgcc
    13801 cggtggcggc tcccgcagca ccggtgagcc actcggagtc agcccgcttc ggtcgggtcg
    13861 acgacaccgg caccgtcttc gtcaccgtcg gtgaggagga gcgcgaggtg gggtcctacc
    13921 cgggggcgtc cgccgaggag gccctgcagt acttcgcgcg caagtacgac gagctggccg
    13981 cgtcggcaga ccttctcgag gcgcggctgc gcaaccccga ggtcgcctcc aaggaggtcg
    14041 ccgacgggtt gacgacgctc aaggagcaca tcgcctccat cggggtcgtg ggtgacctcg
    14101 ccgccctggg ggccaccgtg gcccgggtcg aggagggctt ggccgccaag cgtgccgagg
    14161 aggcgcaggt gcgggcagca gcccgcgcgg tggccgccga gcagcgcgag gcactcgtcg
    14221 ccgaggccga gaagatcgcg gcccagccgg ccggctccac ccagtggaag accagcggtg
    14281 agcgcatgcg agccctgctc gacgagtgga agcagcacca gcgcagcggc cccaagctcg
    14341 acaagaccac cgagtccgcc ctgtggaccc gcttcagcca cgcccgcaac accttcgaca
    14401 agggccgccg cgcctggttc gccgacctcg agacgagccg cgcggacgcg cgctcgacca
    14461 aggaggccct cgtcaccgag gcggagaagc tcgcgacgag caccgactgg gccccgaccg
    14521 cgcgcgcgtt caagcagctg atggaccgtt ggcgcactgc cggtcgcgcc tcgcgcgccg
    14581 aggacgacgc cctctgggag cggttccgca gcgcgcagga cgccttcttc accgccaagg
    14641 acgaggtcgt cgccgccgag gacgagtcct tccgggccaa cctcgtcgtc aaggaggcgt
    14701 tgctcgtcga ggccgaggcg ctgttgccgg tctccgacgt cgaggcggcc aaggcagcgc
    14761 tgcgcggcat ccaggaccgt tgggaggccg ccggcaaggt cccgcgcgcc gacctcgagc
    14821 gggtcgagaa gcggatgcgc cgggtcgagg ccacgatccg cgaggccgac gagcagcgct
    14881 ggaagcgcac caaccccgag gtggcggccc gcgcccgctc gatggtcgac cagctcgagg
    14941 cctcggtggc cgcgacccag gctgacctgg ccaaggccga ggccaagggc gacaccaagg
    15001 cggccgcagc cgcgaccagc aagctggcgg cgcagcagca gtggctggac caggcgcgcg
    15061 ccggcctcga cgagttcagc ggctgacggt cgctgcgtcc ggcagggtgg tcgctcctgc
    15121 gaccacctcg ccggacgcta gtggtctgag cgctttccgc cggtgacccg gtgtgcgtcg
    15181 aagactccgt cgacgctctg cacggctcgc acgacgtggc ccaggtgcgc cgggtcaccc
    15241 atctcgaagg tgaagtgcag cttggccatc cggtcgcgcg aggtggtcac ggcagcggac
    15301 aggatgttga ctccgttgtc ggagatcacc cgggtgacgt cggagagcag ccgcgaccgg
    15361 tcgagggcgt cgacctggag ctggacgagg aaaaggctgc ccgaggtggg ggcccactcg
    15421 acctcgatga tcttctcggg ctgggagagc agcgagtcgg cgttggtgca gtcgcggcgg
    15481 tggaccgaga cgccggtgcc gcgcgtgacg aagcccagga tgtcgtcgcc gggcaccggg
    15541 gtgcagcacc gcgcgagctt gacccacacg tccgacatgc ccttgacgac gaccccgggg
    15601 tcgcctgagc gtcggtggtg ggtgcgccgg gcggtggggc tggctgcctc ggcgatgtcc
    15661 tcgctggcac cctcctctcc cccgaccgag gccacgagcc gcgacacgac gtgctgggcc
    15721 gagacgtggc cctcgccgat ggcggcatag agaccgtcga tcccggccag gcgcagctcc
    15781 tcggcgacgc ccgtgaggga ctcgaccgag gtgagtcgct gcaggggcag gccctgcttg
    15841 cgcatcacct tcgcgagggc gtccttgccc tcgtcgatca tctcctcgcg gcgttccttg
    15901 gtgaaccagt gcttgatctt gttgcgggcc cgtgcggagc gcacgaagtt cagccagtca
    15961 cgcgacgggc cggcggagtc ggccttggac gtcagcacct cgacgacgtc gccgttgtcg
    16021 aggcggctct ccaacggcac gagacgggcg ttgaccctgg cgccgatgca gtggtggccc
    16081 acctcggtgt gcacggcata ggcgaagtcg accggcgtcg acccggccgg cagaccgacg
    16141 agctgtccct tcggcgtgaa gacgtagacc tcgctggcgg tgatctcgaa gcgcagcgac
    16201 tccaggaact cacccgggtc ggaggtctcc ttctgccagt cgagcagctg gcgcaaccaa
    16261 gccatgtcgt tgatcggacc ggtctggccg tcgcgggcct gcgccccgcc ctgagccccc
    16321 cgggcggccg cacccttggc gttgggctct tccttgtact tccagtgggc cgcgacgccg
    16381 tactcggccc ggcggtgcat cgtgtgcgtg cggatctgga tctcgacggg cttgcccccg
    16441 gggccgatga ccgtcgtgtg caacgactgg tacatgttga acttcggcat cgcgatgtag
    16501 tccttgaacc g
//
LOCUS       DAOSOC010000039        16436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3559_length_16436_cov_9.109395, whole genome shotgun sequence.
ACCESSION   DAOSOC010000039 DAOSOC010000000
VERSION     DAOSOC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 16436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16436
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3559_length_16436_cov_9.109395"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PL014_04925"
     CDS             complement(<1..460)
                     /locus_tag="PL014_04925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT56466.1"
                     /translation="MSATVRSVLLAGGGTAGHVSPLLALADCLRRRDPDIAITALGTA
                     TGLEARLVPERGYPMIEVPKVPLPRRPSGDLLRLPGNLRRAVRAAEDAIESTSAEVVV
                     GFGGYVSTPAYLAARRHGIPIVVHEQNARPGIANRLGARIASAVGVTFPDT"
     gene            complement(457..1731)
                     /gene="ftsW"
                     /locus_tag="PL014_04930"
     CDS             complement(457..1731)
                     /gene="ftsW"
                     /locus_tag="PL014_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOT56467.1"
                     /translation="MSSTSAPARARSAGADAGTTTHRGWWGRFESPVTTYYLLVGVTA
                     ALVVFGLIMVLSASAIVSIQETKGASAYSIFISQAVFATIGAVALTVASRLPMRSWKA
                     LAFPILFVGVALQVLVLFTGTAVNGNQNWLKVGPITVQPSEIIKLGLVLTGAVVLSKK
                     RQHLHSFAHVVVPYLVPIVAVGVGAVALGHDLGTVLILGAIVAGVLFAAGVPLRWFAF
                     AGVPFIGGVLWFVLSSANRLSRFDVWLGRDTDEFGTARQPIHGRYALADGGWLGVGLG
                     QSREKWGLLSEPHNDFIFAIIGEELGLPGSLGVLALFAALALACYRLVTRTDDFFVRV
                     ATAGIMTWVIIQAVINIGAVINLLPVIGVPLPLVSSGGSALITTMFALGILLSFARSE
                     PGCAEALSARPSAIRRTLAVLPGVGRRRRARS"
     gene            complement(1734..3203)
                     /gene="murD"
                     /locus_tag="PL014_04935"
     CDS             complement(1734..3203)
                     /gene="murD"
                     /locus_tag="PL014_04935"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT56468.1"
                     /translation="MSERFATLTHRDANWAGLRVVVTGLGVSGFAAADALLERGADVL
                     VVDGAHPDGILAERANILDILGARLAFGPDAVARMPDTPVDLVVTSPGWRPDQPLLAA
                     AAAAGIPVWGEVELAWRMRPEHGGAPWLAVTGTNGKTTTVQMLTSILRAAGLRACSAG
                     NVGTPILEAVVHPDPFDVIAVELSSFQLHWSHSLAPQASVCLNVAPDHVDWHGSLEEY
                     ALAKGKVYANTEVACIYNVQDERTRRLVEEADVQEGCRAVGFTLDLPAVSEVGLVGDV
                     LADRAFVEQRRTAAAELATLDDLANGGPPPAPHLVANALAAAAVARAHGVPAGAVRAG
                     LRAFVPEPHRIAEAATIDGIRFVDDSKATNPHAARASLTAYEHVVWVAGGLLKGADVD
                     DLVAAAAGHLRAVVLIGQDRAAIAQALARHAPDVPVVDIDRTDTGAMDEVVKQARALA
                     RVGDVVLLAPAAASMDMFTDYGARGDAFVGAVDRLAAGE"
     gene            complement(3200..4282)
                     /gene="mraY"
                     /locus_tag="PL014_04940"
     CDS             complement(3200..4282)
                     /gene="mraY"
                     /locus_tag="PL014_04940"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT56469.1"
                     /translation="MRAVLIAGVVSLVISLLGTPLFIRFLVRKGYGQFIRDDGPTSHH
                     TKRGTPTMGGAVIVLASLIAYAVAHLVTGQSPSVSGLLVLFLMTGLGFIGFLDDFIKI
                     SKQRSLGLRSGEKLAGQTLVAVIFAILALQFPNDAFRTPASTSISFVRDTGLDLAFSG
                     ALVGVILFVIWANIIVAGTSNGVNLTDGLDGLATGASVMVFAAYVLIGIWQFNQNCQW
                     NPGTKCYEVRDPHDLAIVAACGMGACFGFLWWNASPAKIFMGDTGSLALGGGLAGLAI
                     LTRTELLIVILGGLFVTITLSVIIQVASFKTTGKRVFRMAPLQHHFELLGWAEVTIVI
                     RFWIIAGLFVALGLGLFYAEWVVGAG"
     gene            complement(4279..5724)
                     /gene="murF"
                     /locus_tag="PL014_04945"
     CDS             complement(4279..5724)
                     /gene="murF"
                     /locus_tag="PL014_04945"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT56470.1"
                     /translation="MIALTLREVAQAVSGVLVAPADVDVDTLVIDGPVVTDSREAGPG
                     GLYVARVGEQADGHDFIPDAVARGAVAALVTRAVDGVPCVVVADTQDAFAALSLEVVR
                     RLPDLEVVGITGSSGKTSTKDLLGSVLATVGPTVAPVGSYNSEVGVPLTVCRVAADTR
                     FLVVEMGARGIGHITYLTTMAPPRIGVVLNVGTAHVGEFGSRAAIATAKSELVAALPA
                     HGLAVLNADDPAVRAMAEVTPARVCLVGEADDAQVRATDVVLDDGGRAAFTVHTPAGT
                     RAIHLGLVGRHHVGNALSVIAVATELGLPLDDVVAALEAARPVSRWRMEVIERRDGVT
                     LVNDAYNANPDSMHAALGALARMGQGRRTWAVLGTMLELGPESDSLHAEVGADVVAHG
                     IGELLVVGAAAASLAAGARAATSGTSGAGGANLPDATTTRVREVPHAEAAQRLLRAEL
                     APGDVVLFKSSRDAGLRLLGDRMAQDEEELT"
     gene            complement(5721..>6766)
                     /gene="murE"
                     /locus_tag="PL014_04950"
     CDS             complement(5721..>6766)
                     /gene="murE"
                     /locus_tag="PL014_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HOT56471.1"
                     /translation="TCVMEVSSHALAQHRVDGVVYDVALFTNLSQDHLDFHDGMEDYF
                     AAKASLFTPQRARRGLVCVDDAWGRRLASEATIPVQTIATLHAGQEATEGSADAAPAA
                     GTGGAGSADWLITVDAADPAAFHLTGPGVDLHLRSRLPGDFNVTNTAMAAAALILLGE
                     DVDAVGAAVLTDPHVPGRMEAVTGPQGSPRALVDYAHTPEAIRAALHALRPSTPGTLV
                     VVTGAGGDRDRDKRHAMGEAAAEVADLVVVTDDNPRSEDPSSIRTALLGGVEAARAGG
                     RHGIRVLTIGDRREAIREAVAAVWSEGGAATVAVVGKGHETGQEIAGVVHPFDDRAEL
                     AHALDLAASSATR"
     assembly_gap    6767..6866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6908..8767)
                     /locus_tag="PL014_04955"
     CDS             complement(6908..8767)
                     /locus_tag="PL014_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT56472.1"
                     /translation="MSSAPPRPVTAPANPRRRMRALTVASLFVLSVFGAQLIRIQGFD
                     AHAVAEEARSKRAGTQVIPAMRGQILAADGTVLAASAVREVVVADQQAVCTYRTKKNT
                     CSPERSAQAVQEAAAGLAPLLGTTVAQLVPKLTGINRYVILSKDVTPVTWNAIADLGI
                     PGIYRDRRETRSERTYPQGTTTAALVGYVTADGEPGGGVELMVKKHLQGTPGKQTFEQ
                     GRDGTIIPVGERSLTPAVDGNDVHLTISSSLQWYAQNSLAQRIKDVGAKSGTVVVMNA
                     KNGNLLSVASYPTFNPNTDVGKKGAKLGNVAFSDVFEPGSTSKIMTVAAALEEGTVTP
                     STPVVIPNRLNRFDASFKDSHDHPTLYRTVAGTLAESSNIGTVLVSETMTPKTLETYF
                     RKFGVGKRSGTGFPGESAGLLAPSEQWSGTQTKTVAFGQGLSVTAIQAASVFQTIANG
                     GVRVAPRLVESLTAADGTVTPAPASTSTRVVSEQTASDVSKMLEGVVSGEGTAKEAMI
                     PGYRVAGKTGTADYYDAAAKRYSGRTASFIGYAPADDPQIVVAVIVHKPIQPFFGGPV
                     AGPVFKDVATYALQELKIPPTGAKPPKITVKVSPEEAQADPTLLRDGRKRSAG"
     gene            complement(8932..9507)
                     /locus_tag="PL014_04960"
     CDS             complement(8932..9507)
                     /locus_tag="PL014_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56473.1"
                     /translation="MSQLAPARPLPRTAARRTTAATPRQLKVVAPPEIRGNGAFLALC
                     VLLLLGGFVGVLMLNTAMAKGSYTLRDLQHRSDELTDTQDQLRHSLDAVSGPGPLAQR
                     ARELGMVKAPTPAFLRLSDGAVLGVAQKAEADTTFSVVTETSVTSSTPARRPAPAATT
                     TTAPDAPKPTASAATPTTSQATPTPTTTESP"
     gene            complement(9504..10484)
                     /gene="rsmH"
                     /locus_tag="PL014_04965"
     CDS             complement(9504..10484)
                     /gene="rsmH"
                     /locus_tag="PL014_04965"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT56474.1"
                     /translation="MSSPGDAAQRHVPVLRDRIVDLLEPALSVEGAICVDATLGMGGH
                     AEAILDRCPSARVIGVDRDEQALALAGERLARFGDRFLGVHAVYDEIADVVEAHTDGV
                     AHGILFDLGVSSLQLDEPDRGFAYRHDAPLDMRMDQSSGITAAEVLNTYSHGDLARIL
                     KTYGEERFAGRIASAVLREREKEPFTTSARLVDVLRAAIPAASQRSGGHPAKRTFQAL
                     RIEVNAELSVWGRAIDAAIDVLAPGGRIAVLSYHSLEDRITKQAFARGATSRTPAGMP
                     VELPELAAYLRLLTRGAEVPDEQEQHDNPRSASARLRAAERLTSSNGAPA"
     gene            complement(10681..11124)
                     /gene="mraZ"
                     /locus_tag="PL014_04970"
     CDS             complement(10681..11124)
                     /gene="mraZ"
                     /locus_tag="PL014_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOT56475.1"
                     /translation="MQEFLGTHTPRLDDKGRLFLPARYRADLSEGLVITTGQENCLYV
                     FPTAEFHRISAQMAAAPSSSKNVRDLVRVFRARAHSDTPDRQGRVTIPAPLRAYAGLE
                     RECTVIGNGSRLEVWDTARWDAYLDEVMPAYSSYSVETEEVVPGL"
     gene            11476..12408
                     /locus_tag="PL014_04975"
     CDS             11476..12408
                     /locus_tag="PL014_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT56476.1"
                     /translation="MQVSASLAAAMNAVIEGKQSVVRTAITVLLAEGHLLVEDVPGVG
                     KTLLAKTLARTIDCRVSRVQFTPDLLPSDITGVSVFNQDVRDFEFRPGAIFANVVVGD
                     EINRASPKTQSALLESMEEGQVTVDGTTYALPRPFIVMATQNPIEMEGTYPLPEAQRD
                     RFMARISMGYPSARAELDMLDLHGGASPLDALQPVTDAATVSAMVRAVREVHTSPSVR
                     QYIVDLTHATRSSSALRLGASPRATIHLLRASRAHAALAGRDHVLPDDVQQVAVPVLA
                     HRLIPTGETQMAHRSTADVVQDLLQRVRVPSPTR"
     gene            12417..13682
                     /locus_tag="PL014_04980"
     CDS             12417..13682
                     /locus_tag="PL014_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT56477.1"
                     /translation="MLPLRRSLTTRGASFVGAAVVLLACGILLGQPDVTRIGVLLLAM
                     TAMGLVLVRRHGLRLQVVRTAVPSRIAIDERAVVTVRVRNGESTASPVVMAEESIDYA
                     LGDRPRFLLRSLRPGETQEVQYTVRSHSRGVHRIGPLGVRVRDPFGLTLRTAALSGDA
                     EVVVLPRVLPLPGARSLGSGIGSEGSVPHMVALHGEDDQTVREYRDGDDLRRIHWPAS
                     ARTGELMVRQEDRPAKRRAVVILDSRSTAHAGSGRASSLEWCVTMAASVTAHVVGAGY
                     AVHLLTADAGSDTGVRHDDSHEEGLETLARVTLQSPESLRAVLHAASVLTSQGGLVVF
                     IGGPIDDVDGRSLAALRQPGSTGAAIVIDPASFDGHPRRGGGDDVASASASVAALHSN
                     GWLATVVDAQTTASLAWGAVVGAQSVGTR"
     gene            13679..15985
                     /locus_tag="PL014_04985"
     CDS             13679..15985
                     /locus_tag="PL014_04985"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HOT56478.1"
                     /translation="MNRARPIEALLAVLATIAVTIPVTTLFTPNTWMRPSLVLVAVVA
                     VTGAGLRTLTSQRPLVVLGQGTALVYAAALLHGRGHLWADLVPTPETGRAFGILLTQA
                     GETVTISAAPAPSNRGTILAISLLVGLTALAVDAIGVTYRSPALAGIPLLAAFLGSAT
                     NSGDGLASWYVIPTALCWLALMGRQSVGSLRAWGTPAPRAAGRAGADPSTSFATTGRI
                     VGVAALAAAVVVPTLIPHLPTTFIADGLGQSSNGRGGGGSSIQLSSTVDIARDLESRS
                     DNPVLRYRSSSPDLQPLRVGVLDDYRRGQWETRQEFSFVPIDGQIPPPVAGPEVPRRV
                     ERIEVSDNAVGAPQVALPAGTISTSLPDGTWNVTVAGVVQATAAADSYTAEFIELDPE
                     PAQFATDADAAQLGVDPSTLALDRRSEDEVRALLAELTDDGDSALEVARTVQAYLRSS
                     QFTYSLELAEDDGTLPADPLLRFLETRRGYCVQFSTAMIMLTRAAGIPSRLAVGFLPG
                     VPDGDDRVVRVSDAHAWPELYFPQLGWVRFEPTPATQSNAAPDYSLQPSDPGAEASPT
                     PSATTPTASAAPSAAPTRDLTDNGGMGAADSGGFSPVRFVADHALTLLGILLVVLAVS
                     AVPFGAWLSRRRAARAARDDAERVEAQWQSLLLRLQDIGYVPPDGTTPRQASRQITKA
                     AYLTPDENEALGRVVATVELARYARPGTELNDVADDAQTVWRGALGRRRRVDKLRALL
                     LPAEGRGLWRSVLRFGRPAPPDRDTHDD"
     gene            complement(16117..>16436)
                     /locus_tag="PL014_04990"
     CDS             complement(16117..>16436)
                     /locus_tag="PL014_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT56479.1"
                     /translation="HTPLGEDGDSEFGDLIEDSEAVVPADAVSFTLLQEQLHSVLDTL
                     SEREAGVVSMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSKLRHPSRSQALRD
                     YLD"
BASE COUNT         2315 a         5695 c         5985 g         2341 t
ORIGIN      
        1 gggtgtccgg gaaggtcacg cccaccgcgg atgcgatccg ggcgccgagg cggttcgcga
       61 tgcccgggcg ggcgttctgc tcgtggacga cgatggggat gccgtgccgt cgcgccgcga
      121 ggtaggccgg cgtcgagacg tagccgccga acccgacgac gacctcggct gaggtgctct
      181 cgatcgcgtc ctcggcggct cgaacggcgc gacgcaggtt gccgggcagc cgcagcaggt
      241 cacctgaggg tcgacgaggc aggggcacct tgggcacctc gatcatcggg tacccgcgtt
      301 cggggaccag ccgggcctcg agcccggtcg cggtaccgag cgccgtgatc gcgatgtcgg
      361 ggtcgcggcg gcgcaggcag tcggccaggg cgagcagcgg cgagacgtga cccgccgtgc
      421 ctcccccggc gagcaggacc gagcgcacgg tggcgctcac gagcgggccc ggcgccggcg
      481 gcccacgccg ggaagaacgg caagggtccg gcggatcgcc gaaggccggg cggacaacgc
      541 ctcggcgcag ccgggctcgg accgggcgaa ggagagcagg atgccgaggg cgaacatcgt
      601 cgtgatgagg gccgagccac cggaggagac cagcggcagg gggaccccga tgacgggcag
      661 gaggttgatg accgcgccga tgttgatgac cgcctggatg atgacccacg tcatgatccc
      721 ggccgtggcg acgcggacga agaagtcgtc ggtgcgcgtg accagccggt agcaggcaag
      781 cgccagcgcc gcgaacaggg ccagcacccc gagcgagccg ggcaggccga gctcttcacc
      841 gatgatcgcg aagatgaagt cgttgtgcgg ctcggacagc agcccccact tctcgcggct
      901 ctgcccgagg ccgacgccga gccacccgcc gtcagcgagt gcgtaacgac cgtggatcgg
      961 ctggcgcgcg gtgccgaact cgtcggtgtc gcgccccagc cagacatcga accgactgag
     1021 ccggttggcg ctgctcagca cgaaccagag cacgccaccg atgaacggca ccccggcgaa
     1081 ggcgaaccag cgcagcggaa ccccagcggc gaagagcacg ccggcgacga tcgccccgag
     1141 gatgaggacg gtgccgaggt cgtgcccgag agccacggcg ccgaccccga cggccacgat
     1201 ggggacgagg tagggcacga cgacgtgcgc gaaggagtgc aggtgctggc gcttcttgga
     1261 cagcacgacg gcgccggtca gcaccaggcc cagtttgatg atctccgagg gctgcacggt
     1321 gatcgggccg accttgagcc agttctggtt gccgttgacg gcggtaccgg tgaacagcac
     1381 cagcacctgc agggccacgc cgacgaacag gatcgggaac gccaacgcct tccaggagcg
     1441 catcggcaac ctcgaggcga cggtgagcgc gacggcaccg atcgtggcga agacggcctg
     1501 gctgatgaag atcgagtagg ccgaagcgcc cttggtttcc tggatgctga cgatcgccga
     1561 ggccgagagc accatgatga ggccgaagac cacgagggcg gcggtcaccc cgacgagcag
     1621 gtagtaggtc gtcaccgggg actcgaaccg cccccaccag ccacggtgcg tcgtcgtccc
     1681 ggcatccgca ccggcgctgc gagcgcgggc cggtgcgctc gtgctgctca cggctactcc
     1741 cccgcggcga gccggtcgac cgcgccgacg aaggcgtcac cgcgagcgcc gtagtccgtg
     1801 aacatgtcca tcgacgcggc agcgggcgcg aggagcacga cgtctcctac ccgtgcgagc
     1861 gcgcgggcct gcttcaccac ctcgtccatg gcaccagtgt cggtgcggtc gatgtcgacg
     1921 acggggacat ccggcgcgtg tcgggccagt gcctgtgcaa tggctgcccg gtcctgaccg
     1981 atgagcacga cggcgcgaag gtgcccggcc gccgcggcga cgaggtcgtc gacgtcggca
     2041 cccttgagca ggcccccggc cacccagacg acgtgctcgt aggcggtgag gctggcgcgg
     2101 gccgcgtgcg ggttggtggc cttggagtcg tcgacgaacc ggatgccgtc gatcgtcgcg
     2161 gcttcggcga tgcgatgggg ttcgggcacg aacgcacgca gcccggctcg caccgcaccg
     2221 gcagggacgc cgtgtgcccg ggccacggcg gcggcggcga gggcgttcgc gacgaggtgc
     2281 ggggccggcg gtgggccacc gttcgcgagg tcgtcgaggg tggcgagctc ggcggccgcg
     2341 gtgcggcgct gctcgacgaa ggcccggtcg gcgaggacgt cgccgacgag cccgacctcc
     2401 gagaccgcgg gcaggtcgag ggtgaagccg acggcgcgac acccctcctg gacgtcggcc
     2461 tcctcgacga gtcggcgggt gcgctcgtcc tgcacgttgt agatgcaggc gacctcggtg
     2521 ttggcgtaga ccttgccctt ggccagggcg tactcctcga gggacccgtg ccagtcgacg
     2581 tggtcggggg cgacgttgag gcacaccgac gcctgcggtg ccagggagtg cgaccagtgc
     2641 agctggaagc tcgagagctc gacggcgatg acgtcgaacg ggtcggggtg gaccacggcc
     2701 tcgaggatcg gggtgccgac gttgccggca ctacatgccc gcagccccgc cgcccgcagg
     2761 atcgaggtga gcatctgcac cgtggtcgtc ttgccgttgg tgccggtcac ggcgagccac
     2821 ggggcgcccc cgtgctccgg cctcatccgc caggcgagct cgacctctcc ccagacgggg
     2881 atccccgccg cagcggcggc cgcgagcagc ggctggtcgg gccgccaccc cggggaggtg
     2941 acgacgaggt cgaccggggt gtccggcatc cgagcgacgg catccgggcc gaaggcgagg
     3001 cgcgcgccga ggatgtcgag gatgtttgcc cgctcggcga ggatgccgtc ggggtgggca
     3061 ccgtcgacga cgagcacgtc ggcgccgcgt tcgagcaggg cgtccgcggc cgcgaaaccg
     3121 ctgaccccga gcccggtgac gacgacgcgc aggcctgccc agttcgcgtc gcggtgggtc
     3181 agggtggcga accgctcgct catccggcgc ccacgaccca ctctgcgtag aacaacccga
     3241 gcccgagcgc gacgaagagg ccggcgatga tccagaaccg gatcacgatc gtgacttctg
     3301 cccagccgag gagttcgaag tggtgttgca gcggtgccat gcggaacacc cgtttaccgg
     3361 tggtcttgaa gctggcgacc tggatgatga ccgagagcgt gatggtgacg aacaggccgc
     3421 cgaggatgac gatgagcagc tcggtgcggg tgaggatcgc gagcccggcc agcccaccgc
     3481 cgagggccag cgacccggtg tcgcccatga agatcttggc gggtgaggcg ttccaccaca
     3541 ggaaaccgaa gcaggcgccc atgccgcagg ccgcgacgat ggccaggtcg tgggggtcac
     3601 gcacctcgta gcacttggtg cccgggttcc actggcagtt ctggttgaac tgccagatgc
     3661 cgatgagcac gtaggccgcg aacaccatga ccgacgcccc ggtggcgagg ccgtcgagcc
     3721 cgtcggtgag gttcacgccg ttcgacgtgc ccgcgacgat gatgttggcc cagatgacga
     3781 agaggatgac gccgaccagg gctccactga acgcgaggtc gagcccggtg tcgcgcacga
     3841 aggagatcga cgtggaggcc ggggtgcgga acgcgtcgtt ggggaactgc agcgcgagga
     3901 tggcgaagat gacggcgacg agggtctggc cggcgagctt ctcacccgag cgcaagccga
     3961 ggctgcgctg cttggagatc ttgatgaagt cgtcgaggaa gccgatgaag cccagcccgg
     4021 tcatcaggaa caggacgagc aggccgctga cgctggggct ctgaccggtg accaggtggg
     4081 ccacggcgta ggcgatgagc gaggcgagca cgatgacggc cccacccatg gtgggggtgc
     4141 cgcgcttggt gtggtgcgag gtgggcccgt cgtcgcggat gaactggccg tagcccttgc
     4201 gcacgaggaa gcggatgaac agcggggtgc cgagcagcga gatgacgagc gacacgacgc
     4261 cggcgatgag cacagccctc acgtcagctc ctcctcgtcc tgcgccatcc ggtcaccgag
     4321 caaccgtagt cccgcgtcgc ggctggactt gaacaacacc acgtctccgg gcgcgagctc
     4381 ggcccgcagc agccgctgcg cggcctcggc gtggggcacc tcgcgcaccc tcgtggtcgt
     4441 tgcgtccggc aggttggcac ccccagcacc cgaggtgccg gacgtagcgg ctcgggcgcc
     4501 ggccgccagg gatgccgcgg cggcgccgac gacgagcagc tcaccgatgc cgtgggcgac
     4561 gacgtcggcc ccgacctcgg cgtgcagtga gtccgactcc ggcccgagct cgagcatcgt
     4621 gccgaggacg gcccaggtgc gccggccctg ccccatcctg gcgagggcac cgagggcggc
     4681 gtgcatggag tccgggttgg cgttgtaggc gtcgttgacc agggtgaccc cgtcgcggcg
     4741 ttcgatgacc tccatgcgcc accggctcac cggtcgggct gcctcgaggg cggccacgac
     4801 gtcgtcgagg ggcagtccca gctcggtcgc gacggcgatc actgacaggg cgttgccgac
     4861 gtggtggcgt ccgaccagcc ccaggtggat cgcgcgggta ccggccgggg tgtgcaccgt
     4921 gaacgcggcc cggcccccgt cgtcgaggac gacgtcggtg gcccgcacct gcgcgtcgtc
     4981 agcctcgccg acgaggcaga cccgggcggg cgtcacctcg gccatggccc gcactgcggg
     5041 gtcgtcggcg ttgagcacgg cgaggccgtg agccggcagg gccgcgacga gctcggactt
     5101 cgcggtggcg atcgctgctc gtgagccgaa ctccccgacg tgcgcggtgc cgacgttgag
     5161 cacgaccccg atgcgcggcg gcgccatcgt cgtcaggtac gtgatgtggc cgatcccgcg
     5221 ggcccccatc tcgacgacga ggaagcgggt gtcggcagcg acgcggcaca cggtcagcgg
     5281 cacgccgacc tcggagttgt acgacccgac gggggcgacc gtgggaccga cggtcgcgag
     5341 gacgctgccg aggaggtcct tcgtgctcgt cttgccggac gagccggtga tgccgacgac
     5401 ctcgaggtcg ggaaggcgac gcacgacctc gagggacagc gcggcgaagg cgtcctgggt
     5461 gtcggcgacg acgacacacg ggacgccgtc gacggcccgc gtgacgaggg ccgcgacggc
     5521 accgcgcgcc acggcatccg ggatgaagtc gtggccgtcg gcctgctcac cgacgcgtgc
     5581 gacgtagagg ccgccggggc ctgcctcgcg cgagtcggtg acgaccggcc cgtcgatcac
     5641 gagcgtgtcg acgtcgacgt cagcgggtgc gacgagcacg ccgctgacgg cctgggcgac
     5701 ctcgcgcagg gtgagggcga tcatcgggtg gcgctcgatg ctgcgaggtc gagcgcgtgg
     5761 gcgagctcgg cccggtcgtc gaaggggtgc acgaccccgg cgatctcctg accggtctcg
     5821 tggcccttgc cgacgacggc gaccgttgcc gcaccgccct cggaccacac cgccgcgacc
     5881 gcctcgcgga tcgcctcccg acggtcgccg atggtgagca cccggatgcc gtgtcgcccg
     5941 ccggcacgcg ccgcctcgac ccccccgaga agggctgtgc ggatcgacga agggtcctcg
     6001 gagcgcgggt tgtcgtcggt gacgaccacg aggtcggcca cctcggcagc cgcttcaccc
     6061 atggcatggc gcttgtcgcg gtcgcggtct cccccggctc cggtgacgac gacgagggtg
     6121 cccggggtgc tcgggcgcag cgcgtggagg gcggcgcgga tggcctcggg ggtgtgggcg
     6181 tagtcgacga gcgcgcgcgg cgaaccctgc gggccggtga ccgcctccat ccggccgggg
     6241 acgtgcggat cggtgagcac tgcggcgccg accgcgtcga cgtcctcacc gagcaggatg
     6301 agcgcggcgg cggccatggc ggtgttcgtg acgttgaagt cacccggaag tctcgacctc
     6361 aggtggaggt ccaccccggg gccggtcagg tggaacgcgg ccgggtcggc ggcgtcgacg
     6421 gtgatcagcc agtccgcgga ccccgctccc cctgtacccg cggcgggcgc tgcgtccgct
     6481 gagccctcgg tggcctcctg gccggcatgg agggtggcga tcgtctggac ggggatcgtg
     6541 gcctcgctcg ccagtcgccg accccacgcg tcgtccacgc agaccagacc gcgccgggcc
     6601 cgctgcggcg tgaacagcga cgccttggcg gcgaagtagt cctccatgcc gtcatggaag
     6661 tccaggtggt cctggctgag gttggtgaac agggcgacgt cgtagacgac cccgtcgacc
     6721 cggtgctggg ccagggcgtg gctggagacc tccatgacgc aggtgcnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnngcgc agcggtgtcg gtcgaggtac ggccacgtcg
     6901 cagcacccta cccggcgctg cgcttgcggc cgtcgcgcag cagggtcggg tcggcctgcg
     6961 cctcctccgg ggacaccttg acggtgatct tgggcggttt ggcacccgtc ggcgggatct
     7021 tcagctcctg cagcgcgtag gtggcgacgt ccttgaacac ggggccggcg accgggccac
     7081 cgaagaacgg ctggatgggc ttgtgcacga tgaccgcgac gacgatctgc gggtcgtcgg
     7141 ccggggcgta cccgatgaag ctcgcggtgc gcccgctgta ccgcttcgcg gccgcgtcgt
     7201 agtagtcggc cgtccccgtc ttgccggcca cgcggtagcc cgggatcatc gcctccttcg
     7261 ccgtgccttc gccgctgacg actccttcga gcatcttgct gacgtcgctc gcggtctgct
     7321 cggagacgac ccgcgtcgag gtcgatgcgg gtgccggtgt caccgtgccg tccgcggcgg
     7381 tgaggctctc gacgagtcgc ggtgcgaccc gcacgccgcc gttggcgatg gtctggaaca
     7441 ccgaggcggc ctggatcgcc gtgaccgaca acccctgtcc gaaggcaacc gtcttggtct
     7501 gcgtcccgct ccactgctcc gaaggggcga gcaggcccgc tgactccccg gggaagccgg
     7561 tgcccgaacg cttgcccacg ccgaacttgc ggaagtaggt ctcgagggtc ttcggcgtca
     7621 tggtctcgct gacgaggacc gtgccgatgt tgctggactc ggcgagggtt ccggcgaccg
     7681 tgcggtacag ggtggggtgg tcgtggctgt ccttgaagga ggcgtcaaag cgattgagcc
     7741 ggttggggat gacgaccggc gtcgacggcg tgaccgtgcc ctcctcgagg gccgcggcga
     7801 cggtcatgat cttcgaggtc gaccccggct cgaacacgtc ggagaaggcg acgttgccga
     7861 gcttggcacc cttcttcccg acatcggtgt tcgggttgaa cgtggggtag gacgcgacgc
     7921 tgaggaggtt gccgttcttg gcgttcatga cgacgacggt gcccgacttc gccccgacgt
     7981 ccttgatccg ctgcgccagc gagttctggg cgtaccactg caggctcgag ctgatggtca
     8041 ggtgcacgtc gttgccgtcg accgcggggg tgagcgacct ctcccccacg gggatgatgg
     8101 tgccgtctcg tccctgctcg aaggtctgct tgcccggggt gccctgcagg tgcttcttga
     8161 ccatgagctc gacgccgccg ccgggttcgc cgtctgccgt gacgtacccg acgagcgccg
     8221 ccgtggtcgt gccctgcggg taggtgcgct cggaccgggt ctcgcggcgg tcccggtaga
     8281 tgccggggat gccgaggtcg gcgatggcgt tccacgtcac cggggtgacg tccttgctga
     8341 ggatgacgta gcggttgatc ccggtgagct tcggcacgag ttgggccacg gtggtgccga
     8401 ggaggggcgc cagccccgcg gcggcttcct ggacggcctg cgccgaccgc tccgggctgc
     8461 acgtgttctt cttcgtgcgg tacgtgcaca ccgcctgctg gtcggcaacg acgacctctc
     8521 gcaccgcgct ggcagcgagg acggtgccgt cggcggcaag gatttggccg cgcatggcgg
     8581 ggatgacctg cgtgccggcg cgcttgctcc gggcctcctc ggccacggcg tgggcgtcga
     8641 agccctggat cctgatgagc tgggcaccga agacactgag cacgaacagg ctcgccaccg
     8701 tcagtgcgcg catccgccgg cgcgggttcg ccggagcggt gacggggcgc ggcggggcac
     8761 tgctcacggg cggcttccgg cgaggtgccg acgaccgggg ctgcgcgggt cggggctgag
     8821 caggtcgtgg cgcggacgac cggggagcag cggggcgtgc tgccgtgcgt cggggagccg
     8881 gagtgccgcc gggtcgcccg ggccctgagg gcggtggcgt cctgcgctgg gtcacgggct
     8941 ctccgtcgtg gtcggcgtgg gtgtcgcctg gctggtcgtg ggggtggctg ccgaagcggt
     9001 gggcttcggc gcatcggggg cagtggtcgt ggtggccgcc ggagcaggcc tgcgcgccgg
     9061 ggtgctggag gtgaccgagg tctcggtgac cacgctaaac gtggtgtcgg cctcggcctt
     9121 ctgcgccacg ccgagcacgg ccccgtcgga caaccggagg aaggcgggcg tgggggcctt
     9181 gaccatcccg agctcccggg cccgttgggc gagcgggccg ggaccggaga ccgcgtcgag
     9241 ggagtggcgc agctggtcct gggtgtcggt gagctcatcg gaacggtgct ggaggtcgcg
     9301 cagcgtgtag gaccccttgg ccatcgccgt gttgagcatc agcacaccga cgaagccccc
     9361 gagcagcagc agcacgcaca gcgccaggaa cgcaccgttg ccacggatct ccggcggcgc
     9421 caccaccttg agctggcgag gggtcgccgc cgtggtccgc cgggcagcag tgcgcgggag
     9481 aggcctggcc ggtgcgagct ggctcatgcg ggggctccgt tcgaggaggt cagtcgttcg
     9541 gctgcgcgca ggcgggccga ggcggatcgg gggttgtcgt gctgttcctg ctcgtccggg
     9601 acctcggccc cgcgggtcag cagtcgtaga taggcggcga gctcgggcag ttcgacgggc
     9661 atcccggccg gggtgcggct cgtggcgccg cgggcaaacg cctgcttggt gatgcggtcc
     9721 tccagcgagt ggtaggacag caccgcgatc cgcccgcccg gagcaagaac gtcgatcgcg
     9781 gcgtcgatcg cgcgccccca caccgagagc tccgcgttca cctcgatgcg cagggcctgg
     9841 aacgtccgct tggccgggtg tccgccgctg cgctgcgagg cggctgggat cgccgcccgc
     9901 aacacgtcga cgagacgggc cgaggtggtg aacggctcct tctcccgctc gcgcaggacc
     9961 gcagaggcga tgcggccggc gaagcgctcc tcgccgtagg tcttcaggat cctggcgagg
    10021 tctccgtggg agtaggtgtt gagcacctcg gcggccgtga tgcccgacga ctggtccatc
    10081 cgcatgtcca ggggcgcgtc gtgccggtag gcgaaacccc ggtcgggttc gtcgagctgg
    10141 agggaggaga ccccgaggtc gaacaggatg ccgtgggcca cgccgtcggt gtgcgcctcg
    10201 acgacgtcgg cgatctcgtc gtagaccgcg tggacgccga ggaagcggtc accgaagcgg
    10261 gccagccgct cccccgccag ggccagggcc tgctcgtcac ggtcgacacc gatgactcga
    10321 gccgacgggc agcggtcgag gatcgcctcg gcgtggcctc ccatgccgag ggtggcgtcg
    10381 acgcagatcg cgccctcgac ggacagagcc ggctcgagga ggtccacgat gcggtcccgc
    10441 agcacaggaa cgtgccgctg tgcggcatct cctgggctgc tcatcgtggt ccttcgtcgt
    10501 cgatcggtgt gcggtggggg agcgatgtgg tggtgcgaag tggtggtgtg cggtggtgcg
    10561 aggtggtggt gcgggtcgac ccggctactg gtccccatcc gatccgggcc cggcgcgggg
    10621 gaagtcgcgt cgggagcggg tcggctggag gccggcggtc gggtcggtgc gggcgagccg
    10681 tcagagtccg gggaccacct cctcggtctc gaccgagtag ctggagtacg cgggcatgac
    10741 ctcgtcgagg taggcgtccc agcgggcggt gtcccagacc tcgagccggc tgccgttgcc
    10801 gatgaccgtg cactcgcgct ccagcccggc gtaggcgcgc agcggcgccg gaatcgtgac
    10861 gcggccctgg cggtcggggg tgtcggagtg cgctcgcgcg cggaacacgc gaacgaggtc
    10921 acgcacgttc ttcgacgagc tgggcgccgc ggccatctgc gcggagatgc ggtggaactc
    10981 ggcggtgggg aagacgtaga ggcagttctc ctgccccgtg gtgatgacca gaccctccga
    11041 gaggtccgca cggtagcggg cagggaggaa gagccgcccc ttgtcgtcga gtcggggcgt
    11101 gtgggtgccc aagaactcct gcactggcgc cacctcctcg acccttcact gcgcccccgg
    11161 atccaccggt ttcccccaca ttactccacc aaggctccac aaggcaccga aattcggctc
    11221 tggatgtgca cagcttcgac atctgcccag ttcagatggg gtggtggaaa gtggaggact
    11281 tttggtggcg gatccccgcg ccgacgacat cggcgactcg tggggcaaga tcgagggcac
    11341 ccggagcacc aactgatcgt tggcaacacc accgacgacg aagggatcac cgtgagcgaa
    11401 ctggacgacc accgacgaac ctcgaccgat tcggatgcgc cggcaccggc gcggggagcc
    11461 tccctcgaca cggtcctgca ggtgtcggcg tcgctggcgg ccgccatgaa cgccgtcatc
    11521 gagggcaagc agtccgtggt ccggaccgcc atcaccgtgc tgctcgccga gggccacctt
    11581 ctcgtcgagg acgtccccgg cgtgggcaag accctgctcg cgaagaccct cgcccgcacc
    11641 atcgactgcc gggtgagcag ggtgcagttc acccccgacc tgctccccag cgacatcacc
    11701 ggggtctcgg tcttcaacca ggacgttcgt gacttcgagt tccggcccgg cgcgatcttc
    11761 gccaacgtcg tcgtcggcga cgagatcaac cgcgcctcgc ccaagaccca gagcgcactc
    11821 ctggagtcca tggaggaggg ccaggtcacc gtcgacggca cgacctacgc gctcccgcgg
    11881 cccttcatcg tcatggccac ccagaacccc atcgagatgg agggcaccta ccccctcccc
    11941 gaggcgcaac gcgaccgctt catggcacgg atctcgatgg ggtacccctc ggcccgggcc
    12001 gagctcgaca tgctcgacct ccatggcggg gcctcccccc tcgacgcgct ccagccggtc
    12061 accgatgccg ccaccgtctc ggcgatggtg cgggccgtgc gcgaggtgca cacctccccc
    12121 tccgtgcgcc agtacatcgt cgacctcacc cacgcgaccc gctcgagcag cgccctgcgc
    12181 ctcggcgcct cgccccgcgc gacgatccac ctgctgcgcg cgtcacgggc ccacgccgcc
    12241 ctggccgggc gtgaccacgt cctgcccgac gacgtccagc aggttgccgt cccggtgctg
    12301 gcccatcggc tcatccccac cggtgagacc cagatggccc accgcagcac cgccgacgtc
    12361 gtccaggacc tgctccagcg agtccgggtc ccctccccga cccgctgagg accacggtgc
    12421 tcccgctgcg acgctccctg accacccgcg gtgcgtcctt cgtcggcgcg gccgtggtgc
    12481 tgctcgcctg cggcatcctg ctcgggcaac ccgacgtgac ccgcatcggg gtgttgctgc
    12541 tcgccatgac ggccatgggt ctggttctcg tgcgccgtca cgggttgcgc ctgcaggtcg
    12601 tgcggaccgc cgtccccagc cgcatcgcca tcgacgagcg tgccgtggtg acggtgcgcg
    12661 tgcgcaacgg cgagtccacg gcatcccccg tcgtcatggc cgaggagtcg atcgactacg
    12721 ccctcggcga ccggcctcgg ttcctgctgc gctccttgcg gcccggtgag acccaggagg
    12781 tgcagtacac cgtgcgctcc cacagtcggg gggtgcaccg catcggtccc ctcggcgtgc
    12841 gagttcggga cccgttcggc ctgaccctgc gcaccgcagc cctgagcgga gacgccgagg
    12901 tcgtcgtgct cccccgcgtg ctgcccctgc ccggggcacg ctcgctcggc agcggcatcg
    12961 gctccgaggg ctcggtgccc cacatggtcg ccctgcacgg agaggacgac cagaccgttc
    13021 gcgagtaccg cgacggcgac gacctgcgcc gcatccactg gccagccagc gcccgcaccg
    13081 gtgagctcat ggtgcgccag gaggaccgac cggccaagcg gcgagccgtc gtcatcctcg
    13141 actcacgctc aaccgcgcac gcggggtcgg gccgagccag ctcgctcgag tggtgcgtca
    13201 cgatggccgc ttcggtcact gcgcacgtcg tcggggccgg gtatgccgtc cacctcctca
    13261 ccgccgatgc cgggtccgac accggggtcc ggcacgacga ctcccacgag gaagggctcg
    13321 agaccctggc tcgcgtcacc ctgcagtcac cggagagcct gcgtgcggtg ctgcacgccg
    13381 cgagcgtgct gacctcgcag ggaggtctgg tcgtcttcat cggcggcccg atcgacgacg
    13441 tcgacggacg atctctcgcg gccctgcggc aaccgggttc caccggcgcg gcgatcgtca
    13501 tcgacccggc atccttcgat ggccacccgc ggcgcggcgg gggcgacgac gtcgcctccg
    13561 cgtcagcgtc cgtcgcggcg ctgcactcca acgggtggct ggccaccgtc gtcgacgcgc
    13621 agaccaccgc gtcgctcgca tggggtgccg tcgtcggcgc ccagagcgtg ggaacccggt
    13681 gaaccgggcc cggcccatcg aggccctgct cgcggtgctg gccacgatcg ccgtcaccat
    13741 cccggtgacc acgctgttca ccccgaacac gtggatgcgc ccgagcctgg tcctcgtcgc
    13801 cgtcgtggcc gtcaccggcg cggggttgcg cacgctgaca agccagcgcc ccctcgtggt
    13861 gctcggccag ggcacggcac tggtctacgc cgccgcactt cttcacggtc gtgggcacct
    13921 gtgggcagat ctggtcccca cgcccgagac gggcagggcc ttcgggatcc tgctcaccca
    13981 ggcaggcgag accgtgacga tctcagcggc tccggcaccc tcgaaccgcg gcacgatcct
    14041 cgcgatcagc ctgctcgtcg gcctgacggc acttgcggtc gacgccatcg gtgtgaccta
    14101 tcgcagtccg gcgctggccg gcatcccctt gctcgcggcc ttcctcggtt cggcaaccaa
    14161 ctccggcgac gggctggcgt cctggtacgt catccccacc gccctctgct ggctcgcgct
    14221 catgggccgg cagtcggtgg ggtcgctgcg ggcgtggggc accccagcgc cgcgcgcggc
    14281 cgggcgggcc ggcgcggacc cgagcacctc attcgcgacc actggccgca tcgtcggcgt
    14341 cgctgccctg gccgctgccg tggtggtgcc gaccctcatc ccccacctgc cgacgacgtt
    14401 catcgccgac ggcctcgggc agagcagcaa cggccgtggt gggggtggat cgagcatcca
    14461 gctgtcgtcg acggtcgaca tcgcccgtga cctcgagagc cggtcggaca acccggtgct
    14521 gcgctaccgc tcgtcctcgc cggacctgca accacttcgg gtgggagtgc tcgacgacta
    14581 ccgcaggggc cagtgggaga cccgccagga gttctcgttc gtcccgatcg acggtcagat
    14641 cccgccgccc gtcgcagggc ccgaggtgcc gcgtcgcgtc gaacgcatcg aggtcagcga
    14701 caacgccgtg ggggcgccgc aggtggccct tcccgccggc accatctcga cctcgctgcc
    14761 ggacggcacg tggaacgtca ccgtcgccgg ggtcgtccag gccaccgcag ccgctgactc
    14821 ctacaccgca gagttcatcg agctcgaccc cgagccagcc cagttcgcca ccgatgccga
    14881 tgctgcgcag ctcggcgtcg atccctcgac cctcgcgctg gaccgccggt ccgaggacga
    14941 ggtgcgggcg ctgctcgccg aactgaccga cgacggcgac tccgccctcg aggttgcccg
    15001 gaccgtgcag gcctacctgc gcagctcgca gttcacctac tcccttgaac tcgccgagga
    15061 cgacggcacg ctccccgccg acccactgct gcgcttcctc gagacccgcc gcgggtactg
    15121 cgtgcagttc agcaccgcga tgatcatgct gacccgcgcc gccggcatcc cgtcacgcct
    15181 ggcagtgggc ttcctccccg gagtcccgga cggtgacgac cgggtggtgc gggtcagcga
    15241 tgcccacgcg tggcccgagc tgtactttcc ccagctcggc tgggttcgct tcgagccgac
    15301 tcccgcgacg cagagcaacg cggcccccga ctacagcctc cagccttctg accccggtgc
    15361 cgaggccagc cccacgccga gcgccaccac ccccacggca tccgcagcgc cctcggcggc
    15421 tccgacgcgt gacctcaccg acaacggcgg gatgggcgcc gcagacagcg gtggcttctc
    15481 ccccgttcgg ttcgtcgccg atcacgccct gacgctgctc ggcatcctgc tcgtcgtcct
    15541 ggccgtctcg gcggttccgt tcggggcgtg gctcagccgg cgacgggccg cgcgagccgc
    15601 ccgcgacgat gccgaacggg tcgaggccca gtggcagtcc ctgcttctgc ggctccagga
    15661 catcgggtac gtgccgcctg acgggacgac cccgaggcag gcctcgcgcc agatcaccaa
    15721 ggccgcctac ctcaccccgg acgagaacga agctctgggc cgcgtcgtcg ccacggttga
    15781 gctggcccgc tatgcccgcc ccggcaccga gctcaacgac gtcgccgacg acgcacagac
    15841 cgtgtggcgc ggggcgctgg gtcgccgccg acgggtcgac aagctgcgag ccctgctgct
    15901 gccagccgag gggcgcggcc tgtggcgcag cgtcctgcgg ttcggacggc ctgctccgcc
    15961 ggacagggac acccacgacg attagcccgc ggtctcaacg cagtcgaacc ccggacgcac
    16021 gcacctgcgg gcggtatcac ctcgaccccg cacacggcga cggccccgcc atcgctcgga
    16081 tggtggggcc gtcgtggtgt cgcgacaggg acgacctcag tcgaggtagt cgcgcagcgc
    16141 ctggctacgg ctcgggtggc gcagcttgct catcgtcttg gattcgatct ggcgaatccg
    16201 ctcgcgggtc acgccgtaga ccttgccgat ctcgtcgagc gtcttgggct ggccgtcggt
    16261 cagcccgaag cgcatcgaca cgaccccggc ctcgcgctcg gagagcgtgt ccagcaccga
    16321 gtgcagctgc tcctgcagga gcgtgaagct cacggcgtcg gcaggcacga ccgcctcgga
    16381 gtcctcgatg aggtctccga actcgctgtc gccgtcctcg ccgagcgggg tgtgca
//
LOCUS       DAOSOC010000040        16255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3610_length_16255_cov_9.210432, whole genome shotgun sequence.
ACCESSION   DAOSOC010000040 DAOSOC010000000
VERSION     DAOSOC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 16255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16255
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3610_length_16255_cov_9.210432"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..2154)
                     /locus_tag="PL014_04995"
     CDS             complement(4..2154)
                     /locus_tag="PL014_04995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOT56480.1"
                     /translation="MTNPPDAVPSPTAPAAPSAALTEAARTALRRLVGRDDADFRDGQ
                     LEAISALVADRSRVLVVQRTGWGKSAVYFVSTALRRAEGAGPTVIISPLLALMRDQIA
                     AAERAGIRAVTMNSANAQDWDEVRGALAADAVDVLLVSPERLNNPRFRDEQLPDLARR
                     CGLLVVDEAHCVSDWGHDFRPDYRRIRDLLAGLPADTPVLATTATANARVVTDVVEQL
                     EVGGRSVLTVRGGLARDSLRLGVLRSMSPEQRLGWLVAHLHELPGSGIVYTLTVSAAE
                     DVAAALSDAGIAVASYTGRTDPADRERLEGALRRNEVKALVATSALGMGFDKPDLGFV
                     VHLGAPSSPVAYYQQVGRAGRATERADVLLLPGPEDKDIWAYFASVSMPREEQAQQVL
                     DTLAAAGRALSTAALETAVDVRRTRLELLLKVLDVDGAVQRVSGGWVSTGVPWTYDAE
                     RYERVAAARRAEQQLMIDYERSDSCRMAFLQETLDDDTAVACGRCDGCAGPWYPTSVP
                     DAVVGAARERLTRAGVDLEPRVQWPTGMDRIGVPVKGKISADESLQPGRALARLSDLG
                     WGQRLREVLRDDSPASAELLRACVPVLAEWPWAQRPVAVVAVPSRSHPTLVTSLAAGL
                     AQIGRLPLLGSLDLAHGGPIGEPGGNSAFRLSGVWERFVVGPELAAALAGVNGPVLLV
                     DDLVSSRWTLTVAGRALRQTGVPAVLPFALAVDG"
     assembly_gap    2300..2399
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2400..2676)
                     /locus_tag="PL014_05000"
     CDS             complement(<2400..2676)
                     /locus_tag="PL014_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56481.1"
                     /translation="MGLADTLAYRITAPNVAQRGVRRAASTRVGSRLLARHLPAMDRT
                     VARLSRGRTTAIELLAGLPIIELTTTGRRSGAGDGRDDLRRLPEVPDP"
     gene            complement(2682..4085)
                     /locus_tag="PL014_05005"
     CDS             complement(2682..4085)
                     /locus_tag="PL014_05005"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT56482.1"
                     /translation="MPLLPSTVTRLDHVLATAQRTGRLPSVTAALVRGGELVWSGAVG
                     TVDGRADGRPADADTQYRMGSITKTFIAVGVLRLRVAGRLDLTDRFGAHVPGSALDDV
                     TIEQLLTHASGVQAETNGPWWERTQGGDWDSLAAATGPRFRAGRRFHYSNVGYAALGR
                     LLEVHHGRGWFDVVQDELLEPLGMTRTTTRPTGSTAQGLAVHPFADVLLPEPEHDAGA
                     MAPAGQLWTTATDLARWAAFLGGNTHGLLEADTLSEMVEPHHVVDEPGQPWSGAHGLG
                     WQVWNANGIRYAGHGGSMPGFLAGLRVQLTTDSRTTASGCRPRAVGASGDGVVVLTNT
                     TASAVTKTIGPDLLSTLAELEPAPVTPWSAAGDASLLELVGTWHWGPAVEVVTIVGEH
                     LVLGEPGAGRGSRFRRTGPDAWEGLDGYHTGEPLRVVRHPDGSVSHLDLASFRFTRTP
                     YDPAADVPGGVDDSGWR"
     gene            4682..5188
                     /locus_tag="PL014_05010"
     CDS             4682..5188
                     /locus_tag="PL014_05010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT56483.1"
                     /translation="MSDPAQPASAHPAAEPPLAPDAEPYRVDVPLRWSDMDAYGHVNN
                     VQFLRLLEDARVVAIREWFLGRPSMLDEGIVVSRHAIEYRAPLTFRPAPVEVAMWVSH
                     VHGAGFDLGYVVRDPAAVGDAVYAVAETGLVLYDFSTARPRRLSPEVRAELTRHSGEP
                     VSFRWAGR"
     gene            5185..5862
                     /locus_tag="PL014_05015"
     CDS             5185..5862
                     /locus_tag="PL014_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56484.1"
                     /translation="MTDTLEFVDEQSLEDLGRYAARARAVDAEGGMRLQAAGGVLAAW
                     VGVLPGSGILAEGTVLGLRTFALATPADLDVIVPLGAISDRTHRGAGREFPIPPTRAL
                     APWSALTPPRGPWEPAGALPGDLLAAVARDGLVEVSDAVRERGAAGGFVRDRVWARDV
                     REASGPHPDLLTVDEGMPDASTVVRAGGAFAAYALGFLAPGEPVSVFRSNRWTRLTAP
                     GGHVLLR"
     gene            complement(5915..6730)
                     /locus_tag="PL014_05020"
     CDS             complement(5915..6730)
                     /locus_tag="PL014_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOT56485.1"
                     /translation="MRASDFAYLDAPTPVALAHRGGAAYEPNRGVENTMAAFRRAVEM
                     GYRYLETDVHATKDGQLVAFHDTVLDRVSDVSGAVEDLPYNALREVRIGGTEPIPLLR
                     ELFEAFPGVRVNIDIKADGALEPTVREVLAHDARDRVCIGSFSPRRLRAARSALGPRV
                     ATAAGQVGTALMRLTPAVVSRLLHTPAPVLQIPSQHRVGGRTITLVTPGLVRRAHDLG
                     KHVHVWFHSWSREDATEMHRLLDLGVDGIVTDHIDVLRDVLAERGHPLTAPTH"
     gene            complement(6732..8525)
                     /locus_tag="PL014_05025"
     CDS             complement(6732..8525)
                     /locus_tag="PL014_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOT56486.1"
                     /translation="MTASTPKPLTTPGDVVHPADSPDAPWWRDAVIYQIYPRSWADAD
                     GDGIGDLPGITARLPYLRDLGVDAVWLSPFYVSPMHDAGYDVADYRDIDPRFGTLADA
                     DAMIAQAHDLGLKVLVDLVPNHTSSEHAWFQAALAAGPGSPERDRYVFREGRGEHGEL
                     PPNNWPSVFGGQGWTRVTEADGSPGQWYLHIFDTTQPDLNWHHPEVRQEFLDILRFWC
                     DRGVDGFRVDVAHGLVKRDGLPDWDGPVGLYDEVGDDAGDGTSPAQVHSSGGEVLDHT
                     ATGAAPMWDQDGVHEIYRDWRAVLDSYGAPDRILCAEAWVTPESRLARYVRPDEMHQA
                     FNFDFLDARWDATAISAVIEDSLRSNDNVGAPTTWVLSNHDVVRHASRLGLDQNLPRP
                     NGITADDPQPDAVLGLRRARAATALMLALPGGAYVYQGEELGLPEHTTMPADVRQDPT
                     FHRTEGKVTGRDGCRVPMAWAKDEPSFGFGPGSTPWLPQPQVYGDLAVDQQQGVEGST
                     LELYRDLLAYRRKHRFGHGSLTWDALNSSSVIAFRNTSGDGERTLLVVTNVGDSPVTL
                     PEGDVLISSGPLADDGSLPSDTTAWVRLPVA"
     gene            complement(8614..9021)
                     /locus_tag="PL014_05030"
     CDS             complement(8614..9021)
                     /locus_tag="PL014_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HOT56487.1"
                     /translation="MSLYDEIGGEETFERLVHAFYEGVAADAELRALYPEADLGPAER
                     RLRMFLIQYFGGPTTYSQERGHPRLRMRHAPYPVTLDMRDRWMRHMLAAMDTLDLPEP
                     QAEQMRDYFLRAAHMLVNSDDGIPPTAGHGIPS"
     gene            complement(9018..9938)
                     /locus_tag="PL014_05035"
     CDS             complement(9018..9938)
                     /locus_tag="PL014_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOT56488.1"
                     /translation="MDPVAPVLPTWEQTQQWLLTDGARILLTIVIAMTALWALRRVID
                     RVVATMTSRSARRLAESGRAGRVLASATGLDNERQRQRVETVGSLLRSVVTLTVATLA
                     ILTIMALLGIPLGPLLASAGVAGVALGFGAQSLVKDFLSGVFMIIEDQYGVGDVIDTG
                     EAIGTVEEVTLRITRLRDANGVTWYVRNGEIIRIGNRSQGVATATVDMPVSYAENVDR
                     VVGIIREAAAELGADPDWQEQLIEPPTVLGVESIIGTTMTVRTVITCAPGEQFAVQRE
                     LRERLKNALDSAGVMGPPAAPFIGPTGGKP"
     gene            complement(9945..12497)
                     /gene="pepN"
                     /locus_tag="PL014_05040"
     CDS             complement(9945..12497)
                     /gene="pepN"
                     /locus_tag="PL014_05040"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOT56489.1"
                     /translation="MPGTNLTREEAAARAALVTVDTHEVELDVTTGPETFATRSTIRF
                     ACAEPGSETFVDFVGDAVTGLTLNGTELDPAAHCADSRITLPRLAADNLVVITATGRY
                     TNTGEGLHRFVDPVDDEVYLYSQFEVPDSRRMYPVFEQPDLKAAFTFTVTAPAHWQVV
                     SNSPTPDAEPTGEGVATWRFSPTPRISSYITALVAGPYDVVRDTLTSRRGEVPLGIFC
                     RKSLTPYLDADNLFDLTKRGFAFFEAEFDCAYPFEKYDQIFTPEYNMGAMENAGAVTF
                     NEVYVFRSRVTEALVERRALTLLHELAHMWFGNLVTMRWWNDLWLNESFAEWASTTCQ
                     AEATAWTDAWTTFGTHEKDWAYRQDQLSTTHPIVAEIRDLRDVEVNFDGITYAKGASV
                     LKQLVAHVGREPFRDGLRAYFAKHAWGNTTLDDLLVELEATSGRDLRAWSRLWLETAG
                     VSTLRPVLEVDDRGLIASAVIEQTCAPGFETVRPHTLNVGLYAVRDGALHRTDVIAVD
                     VDGAVTEVAELVGREQPDLLLVNDDDLAYAKIRLDERSLATALTHPRGFTSSLPRSLV
                     LACLWDMTRDAEMGARAFVDVVLATLPGETDSTLLRTLIAQLQTSVLSYTDPEQREAT
                     RASTRDRLWEIARASEPGTDAQLQLVSAACALTTAGDDVASVRALLDGTEVLDGLKVD
                     FEMRWTLLTALAAAGEADDAQIDAELASEDTATGRERAARAKAARPTVQAKEAAWAAA
                     VEGSGLPNAVVDAIGQGFSRPGTPADLLRPYVERYHCMLDTVEERGSHALVESVVYGF
                     YPRPLVDRDLHDATQAWLDTHPDAPAALRRLVVENRDPVARALAAQARDAGA"
     gene            12716..13366
                     /locus_tag="PL014_05045"
     CDS             12716..13366
                     /locus_tag="PL014_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOT56490.1"
                     /translation="MPDTTEKTALTTSAERASAEFWFDPLCPWAWMSSRWLGEVQQVR
                     PVDVTWSVMSLAVLNEGRDLPPQYAELMEQGWGPVRVVIAAREAHGEQVVKPLYDALG
                     EHIHHREEDYSTAIAGALADVGLPAELAEAATSDALDDVLRASHQRAIDLVGDEVGTP
                     VIAVDGVAFFGPVVSPAPRGEAAGRLWDGCVLVAGTDGFFELKRSRTREPIFHTEA"
     gene            13368..13841
                     /locus_tag="PL014_05050"
     CDS             13368..13841
                     /locus_tag="PL014_05050"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HOT56491.1"
                     /translation="MRVHLGGDHAAHDLLLDLVAFLREQGHDVTNHGPHEYDALDDYP
                     VFVLRAAEAVAADPGSFGVVLGGSGNGEQMAANKVTGIRAALCYNDELASLAREHNDA
                     QVISIGARMNTVEQARSMVQTFLATPFSGDPRHVRRITMVADYDESGTLPPLPSA"
     gene            13981..15087
                     /locus_tag="PL014_05055"
     CDS             13981..15087
                     /locus_tag="PL014_05055"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOT56492.1"
                     /translation="MPNDVIVRPRPRAYPAMRWLGGSRRAGSVSERALLVSFVGGLLA
                     LGAAIARWPHAVPISFLFPTVVVAGVVLRPRPLVMVYGIALVLVLGWVPHSGVGLTRS
                     MFVAVSVGAVMAVMLIASVSRARIGTRGFRGDRMFAELRDRIALGGQLPDLPPGWQVS
                     SCIRSAHGEHFSGDFLVAHRSPSGDRLEIVLVDVSGKGTRAGTRSLLLSGAFGGLLGA
                     VPSDQFLATANDYLARQGWVEGFATAVHLEVDLRTGEYAIGNAGHPSAALFTFGKGRW
                     EVIAGGRGPLLGVVPGAHFPRQRGWLGHGDTLLLYTDGIVEARGRDLVDGVDRMLGLA
                     TTTLLSQGDVAREVCSAARSGEDDDRAALAVRRL"
     gene            15120..15830
                     /locus_tag="PL014_05060"
     CDS             15120..15830
                     /locus_tag="PL014_05060"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HOT56493.1"
                     /translation="MPETEPSIDAAAEADPLPAPPNGIRTFTPRWRNSPLTDERMARL
                     MPARALPEGPLVPEVAFGRTGPVVVEIGSGHGAAAIAFCAAHPEADLIAVEVHVPGVA
                     RMLALAEDAGVENLWVHPGDALPFLTDRVAPGALAAVHLFFPDPWPKNKHAKRRFVQQ
                     HTLALIASRLEVGGQLLIATDIDSYAAHSRAQLAEHGRWDVVEGVRPPWRPDAGFEAK
                     GVRAGRVVSELRATLRPL"
     gene            complement(15998..16074)
                     /locus_tag="PL014_05065"
     tRNA            complement(15998..16074)
                     /locus_tag="PL014_05065"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16038..16040),aa:Pro,seq:tgg)
BASE COUNT         2171 a         5653 c         5973 g         2358 t
ORIGIN      
        1 cgctcagccg tcgacggcga gcgcgaaggg cagcacggcg gggaccccgg tctgacgcag
       61 cgcccgcccg gcgacggtga gggtccaccg cgacgagacg aggtcgtcga cgagcaggac
      121 cgggccgttg acaccggcca gggccgccgc cagctctggt ccgacgacga agcgctccca
      181 gaccccggag agccggaagg cgctgttgcc gccgggctcc ccgatgggcc ctccgtgggc
      241 gaggtccaac gacccgagca gcggcaaccg accgatctgc gccagcccag cggcgagcga
      301 ggtcaccaat gtcgggtgtg agcgcgacgg gacggccacg acggcgaccg gccgctgcgc
      361 ccacggccac tcggccagca cgggcacgca cgcccgaaga agctcagcgc tggccgggct
      421 gtcgtcacgc agcacctcac gcaggcgctg accccacccc aggtcggaca gccgagccag
      481 ggccctcccc ggctggagcg actcgtcggc gctgatcttg cccttgaccg gcaccccgat
      541 gcggtccatc cccgtcggcc actgcacccg cggctcgagg tcgacgcccg cgcgcgtgag
      601 gcgttcacgc gccgcgccga caacggcatc cggcacgctc gtcgggtacc acggcccggc
      661 gcagccgtcg cagcgcccgc acgcgacggc ggtgtcgtca tcgagggtct cctggaggaa
      721 ggccatccgg caggagtcgc tgcgctcgta gtcgatcatc agctgctgct cggcccggcg
      781 ggcagcagcg acccgttcgt agcgctcggc gtcgtaggtc cacggcaccc ccgtggacac
      841 ccacccgccc gacacgcgct gcacggcacc gtcgacgtcg agcaccttga gcagcagctc
      901 caggcgggtg cgtcgcacgt cgaccgcggt ttccagcgcg gccgtcgaca gggcccgccc
      961 cgctgcggcc aaggtgtcga gcacctgctg cgcctgctcc tcacgcggca tcgacaccga
     1021 cgcgaaatag gcccagatgt ccttgtcctc ggggccgggc agcagcagca cgtcggcacg
     1081 ctcggtggca cgtccggcgc ggccgacctg ctggtagtac gccaccggcg agctcggtgc
     1141 cccgaggtgc acgacgaagc cgaggtcggg cttgtcgaac cccatcccga gcgcggacgt
     1201 cgcgacgaga gccttgacct cgttgcggcg cagcgcgccc tcgaggcgct cgcgatcggc
     1261 ggggtcggtg cgaccggtgt acgacgcgac cgcgatcccg gcatccgaca gcgccgctgc
     1321 cacgtcctca gccgcggaga cggtcagcgt gtagacgatg ccgctgcccg gcagctcgtg
     1381 caggtgggcg acgagccagc ccagtcgctg ctcgggggac atcgatcgca gcaccccgag
     1441 gcgcagtgag tcacgagcca gaccgccgcg cacggtgagc acggagcggc caccgacctc
     1501 gagctgctcg acgacgtcag tcaccacgcg cgcgttggcg gtggcggtcg tcgccagcac
     1561 cggggtgtcg gccgggagcc cggcgagcag gtcgcggatg cgccggtagt cggggcggaa
     1621 gtcgtgcccc cagtcgctga cgcagtgcgc ctcgtcgacc acgagcagac cgcaccggcg
     1681 cgcgaggtcg ggcagctgct cgtcgcggaa cctggggttg ttcagccgct cggggctgac
     1741 gagcagcacg tcgacggcat cggccgcgag tgccccacgc acctcgtccc agtcctgggc
     1801 gttggccgag ttcatcgtca cggcgcggat gccggcccgc tcggctgccg cgatctggtc
     1861 tcgcatgagc gccaggagcg ggctgatgat gacggtgggc ccggcgccct ccgcccgtcg
     1921 cagcgcggtc gacacgaagt agacggccga cttgccccac ccggtgcgct ggacgacgag
     1981 cacccgtgac cggtcggcga ccagcgccga gatggcctcg agctgcccgt cgcggaagtc
     2041 ggcgtcgtcg cgcccgacga gccggcgcag cgctgtgcgc gcggcctcgg tgagcgcggc
     2101 ggacggtgcg gcgggagcgg taggggacgg gacggcatcc ggggggttcg tcatgatcac
     2161 cccaccgtag gcgcagtcac cgacagcggc gtcccgtcat ccacagggct cgaggacgaa
     2221 gatccgcacc gtccgcccgg agatgcgggt caggtacttc gggtagccga cgtagatcgt
     2281 cgcggccgtc gccagcaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     2401 cgggtcaggt acttcgggta gccgacgtag atcgtcgcgg ccgtcgccag caccggagcg
     2461 ccgacccgtc gtcgtcagct cgatgatcgg caggcccgcg agcagctcga tcgccgtggt
     2521 gcggccccgg ctgaggcggg cgacggtgcg atccatggcc ggcaggtggc gcgccagcag
     2581 tctggacccg acacgtgtgg atgccgctcg ccgcaccccg cgctgagcca cgttcggtgc
     2641 ggtgatgcgg taggcgaggg tgtccgccag gcccacgcgc gtcaccgcca gcccgagtcg
     2701 tccacgccac cgggcacgtc ggctgccggg tcgtagggcg tgcgggtgaa tcggaacgac
     2761 gcgaggtcca ggtgcgagac gctgccgtcg gggtggcgga ccacgcgcag cggttcaccc
     2821 gtgtggtacc cgtcgaggcc ctcccacgcg tcgggcccgg tccgccggaa ccgcgatccc
     2881 cgcccggcac cgggctcgcc gagtacgagg tgctcgccga cgatggtcac gacctcgacc
     2941 gccggccccc agtgccacgt ccccacgagc tcgagcaggc tggcatcgcc cgcggctgac
     3001 cacggcgtca ccggtgccgg ctcgagctcg gccagcgtgc tcagcagatc agggccgatc
     3061 gtcttcgtga cggcgctcgc cgttgtgttg gtgagcacga cgaccccgtc gccggaggca
     3121 cccacggcgc gcggacggca tccggatgcc gtggtgcggc tgtcggtggt cagctggacg
     3181 cgcagccccg cgaggaaccc cggcatcgac cctccgtggc cggcgtaccg gatgccgttg
     3241 gcgttccaga cctgccaccc caggccgtgt gcccctgacc acggctggcc cggctcgtcg
     3301 acgacgtggt gtggctcgac catctcggac agcgtgtccg cctcgagcag cccgtgggtg
     3361 ttgccgccga ggaaggcggc ccaccgtgcc aggtcggtgg cggtggtcca cagctggccg
     3421 gccggggcca tcgcgccggc gtcgtgctcg ggctcgggaa ggaggacgtc ggcgaagggg
     3481 tgcaccgcga ggccctgcgc ggtggaaccg gtcggacggg tcgtcgtgcg cgtcatgccg
     3541 agtggctcga gcagctcgtc ctggacgacg tcgaaccagc cgcggccgtg gtggacctcg
     3601 agaaggcgcc cgagggcggc gtacccgacg ttggagtagt ggaaccggcg cccggcccgg
     3661 aaccgcggac cggtcgcggc ggccagcgaa tcccaatcac cgccttgggt gcgttcccac
     3721 cacggaccgt tcgtctcggc ctggactccg ctcgcgtggg tgagcagctg ctcgatggtg
     3781 acgtcgtcca gggcgctgcc gggcacgtgg gcgccgaagc ggtcggtgag gtcgaggcgg
     3841 ccggcaaccc gaaggcgcag gacgcccacg gcgatgaacg tcttggtgat ggaacccatc
     3901 cggtactgcg tgtcggcgtc ggccgggcgt ccgtcggcgc gaccatcgac cgtgccgacg
     3961 gccccgctcc agacgagctc gccaccacgc acgagcgcgg cggttaccga gggcaggcgg
     4021 ccggttcgct gggctgtggc gagcacgtgg tcgagacggg tcacggtcga ggggagcaac
     4081 ggcatccgca cagcgtaggg cggcaggtgc gacctggcag ccgggaagag agggctggcg
     4141 cggtggtgct gttggccgcg gggaggaggg gggattctgt gggggagggg ctgggggtgc
     4201 cgcgggggga cgtccggatg cttcacgtgg tgagccaccc cccgacacgt ggcgagccac
     4261 cccccgacac gtggcgagct gggttggtgt gccgcgcatc gacctgggcg ccgccgcagc
     4321 ccggctcctg atccgcacac cggtgcaacc tggcctgtga cgtgaaccat cccaaagttg
     4381 catcgcgggg tgagccagtt ggcggcggga ggtgcgcctg ggcagtgtgc ctcggccatg
     4441 cgctgggatg gcgcgtcggg gtggctcacc atgtgaagca tccggacgtc tccccagacc
     4501 acccgcgcac ccgctctcag cattcagggc gatccgacct cagtccgacg ggtgtccacg
     4561 tccaggctgg ctcgtccgac ccggaggcag ggccgccacg tccggcgctg agccggttgt
     4621 ccacccccca agccagtcga ccactcggcc acgaccctgc gcgggctgcg aggatggggg
     4681 catgtcagac ccagcgcagc ccgcctcggc gcaccccgcc gcagagccgc cccttgcccc
     4741 ggatgccgag ccctaccggg tcgacgtccc cctgcgctgg tcggacatgg acgcctacgg
     4801 ccacgtcaac aacgtgcagt tcctccgcct gctcgaggac gcccgggtcg tcgcgatccg
     4861 cgagtggttc ctcggccgcc cctcgatgct cgacgagggc atcgtcgtct cgcgccacgc
     4921 catcgagtac cgcgccccgc tgaccttccg ccccgctccg gtcgaggtgg cgatgtgggt
     4981 cagccacgtg cacggcgccg gcttcgacct cggctacgtc gtgcgggacc cggcagcggt
     5041 cggtgacgcc gtgtatgcgg tcgccgagac cggcctcgtg ctctacgact tctcgacggc
     5101 ccgcccccgg cgcctctcac ccgaggttcg tgccgaactg acccgccatt ccggcgagcc
     5161 cgtgagcttc cggtgggccg gccgatgacc gacacgctgg agttcgtcga cgagcagtcg
     5221 ctcgaggacc tcgggcgcta cgcggcccgc gcccgggccg tcgatgccga gggcggcatg
     5281 cgcctccaag ccgccggggg agtcctggcc gcgtgggtgg gggtgcttcc cggctccggc
     5341 atcctcgccg agggcaccgt cctgggtctg cgcaccttcg ccctcgcgac ccccgccgac
     5401 ctcgacgtca tcgtcccgct cggggcgatc agcgaccgca cccaccgcgg tgctggccgc
     5461 gagttcccga tccccccgac ccgagccctc gcgccgtggt cggcgctgac cccgccgcgc
     5521 ggcccctggg agccggccgg cgccctgccc ggtgacctcc tcgccgccgt ggcccgcgac
     5581 gggctcgtcg aggtgtcgga tgccgttcgc gagcgcggtg ccgccggcgg tttcgtgcgt
     5641 gatcgggtct gggcgcgtga cgtccgggag gcgtccggcc cgcaccccga cctgctcacc
     5701 gtcgatgaag ggatgccgga cgcgagcacc gtggtgcgcg ccggtggcgc gttcgctgcg
     5761 tacgctctgg gcttcctggc gcccggggag ccggtgagcg tcttccggtc gaaccggtgg
     5821 acccggctca ccgcacccgg cggtcacgtc ctgctccgct gacctgacct tgcgtccggc
     5881 acggtggctg cttcagccac caccctgccg gacgtcagtg ggtgggtgcg gtgagcgggt
     5941 ggccgcgctc ggccaggacg tcacgcagca cgtcgatgtg gtcggtgacg atgccgtcga
     6001 ccccgaggtc gagaaggcgg tgcatctcgg tcgcgtcctc gcgcgaccac gagtggaacc
     6061 acacgtgcac gtgcttgccc aggtcatggg cccgccgcac caggcccggg gtgaccaggg
     6121 tgatcgtgcg cccaccgacc cggtgctggc tggggatctg cagcaccggg gcgggcgtgt
     6181 gcagcagccg agacacgacc gcaggggtga gccgcatcag ggccgtgccc acctgcccgg
     6241 cggccgtggc gaccctcggc ccgagagccg accgggcggc ccgcagccgc cgcggcgaga
     6301 acgacccgat gcacacgcgg tcgcgcgcat cgtgggccag cacctcgcgc accgtcggct
     6361 cgagcgcacc gtcggccttg atgtcgatgt tcactcgcac gccggggaag gcctcgaaca
     6421 gctccctgag cagcggtatc ggttcggtgc cgccgatccg cacctcgcgc agcgcgttgt
     6481 acggcaggtc ctcgacggcg cccgacacat cggagacccg gtccaggacg gtgtcgtgga
     6541 aggccacgag ctgcccgtcc ttggtcgcgt gcacgtcggt ctcgaggtag cggtagccca
     6601 tctcgacggc ccgccggaag gccgccatcg tgttctcgac gccgcggttg ggctcgtagg
     6661 ccgccccgcc ccggtgcgcg agggcgaccg gggtgggggc gtccaggtag gcgaagtcgc
     6721 tggcacgcac ctcaggccac cggcaaccgg acccacgcgg tggtgtcgct cggcaggctc
     6781 ccgtcgtcag cgagcggacc cgaggagatg agcacgtcgc cctcgggcag ggtgacgggg
     6841 gagtcgccga cgttggtcac gacgagcagg gtgcgctcgc cgtcacccga ggtgttgcgg
     6901 aacgcgatga cgctcgacga gttcagggcg tcccacgtca ggctgccgtg cccgaagcgg
     6961 tgcttgcggc gataggcgag caggtcgcgg tacagctcga gcgtcgaacc ctcgaccccc
     7021 tgctgctggt ccacggccag gtcgccgtac acctgcggct gcggcagcca cggtgtcgaa
     7081 cccgggccga acccgaagct cggctcgtcc ttggcccagg ccatcggcac gcggcagccg
     7141 tcgcgcccgg tcacctttcc ctcggtgcgg tggaacgtcg ggtcctggcg gacgtcagcc
     7201 ggcatggtcg tgtgctcggg caggccgagc tcctcaccct ggtagacgta ggcgccaccg
     7261 ggcagggcca gcatcaacgc ggtcgccgcg cgcgcccgac gaaggccgag cacggcatcc
     7321 ggttgcgggt cgtccgccgt gatgccgttg gggcgcggca ggttctggtc gagcccgagc
     7381 cgcgaggcgt gccgcacgac gtcgtggttg gagagcaccc acgtcgtcgg ggcgccgacg
     7441 ttgtcgttgc tgcgcagcga gtcctcgatg accgcgctga tcgccgtggc gtcccagcgg
     7501 gcgtcgagga agtcgaagtt gaaggcctgg tgcatctcgt ccgggcggac gtagcgggcc
     7561 aggcgtgact cgggtgtcac ccaggcctcc gcgcacagga tgcggtcggg ggcgccgtag
     7621 gagtccagca ccgcccgcca gtcacggtag atctcgtgga caccgtcctg gtcccacatc
     7681 ggggcggcac cggtggcggt gtggtcgagc acctcgccac cgctggagtg cacctgcgca
     7741 gggctggtgc cgtcaccggc gtcgtcgccc acctcgtcgt acagaccgac cggcccgtcc
     7801 cagtccggca ggccgtcgcg cttgacgagt ccgtgcgcga cgtcgacccg gaagccgtcg
     7861 accccccggt cgcaccagaa gcgcaggatg tcgaggaact cctggcggac ctcggggtgg
     7921 tgccagttca ggtcgggctg ggtggtgtcg aagatgtgca ggtaccactg gccgggcgag
     7981 ccgtcggcct cggtgaccct ggtccagccc tgcccaccga acaccgacgg ccagttgttc
     8041 ggcggcagct cgccgtgctc accgcggccc tcgcggaaga cgtaccgatc gcgctcgggg
     8101 ctgcccggcc cggccgcgag ggcggcctgg aaccacgcgt gctcactgct cgtgtggttg
     8161 ggcacgaggt cgacgagcac cttgagcccg aggtcgtggg cctgcgcgat catcgcgtcg
     8221 gcgtcggcca gggtgccgaa acgggggtcg atgtcgcggt agtccgccac gtcgtagccg
     8281 gcgtcgtgca tcggtgagac gtagaacggg ctcagccaca cggcatccac cccgaggtcg
     8341 cgcaggtacg gcaggcgcgc cgtgatgccg ggcaggtcac cgatgccgtc gccgtcggcg
     8401 tccgcccacg agcgcggata gatctggtag atcaccgcat cgcgccacca gggggcgtcg
     8461 ggggagtccg ccgggtggac gacgtcgccg ggggtggtca ggggcttcgg ggtgctcgca
     8521 gtcaccggca ccatgtttcc ctacctcgcg ccgatggcga agaccaacgc gcgatccggg
     8581 cgcaggagtg cggcccggat cgtcgtcggc gcgtcaggag gggatgccgt ggccggcggt
     8641 cggggggatg ccgtcgtcgg agttcacgag catgtgcgcc gcccgcagga agtagtcacg
     8701 catctgctcg gcctggggtt cgggcaggtc gagggtgtcc atcgcggcga gcatgtgccg
     8761 catccagcgg tcacgcatgt cgagggtgac ggggtagggc gcgtggcgca tccgcagacg
     8821 ggggtggccg cgctcctggc tgtaggtcgt cgggccgccg aagtactgga tgaggaacat
     8881 ccgcaggcga cgctcggcgg ggccgaggtc ggcctcgggg tagagcgccc gcagctcggc
     8941 gtcggcggcc acgccctcgt agaaggcgtg gacgagtcgc tcgaaggtct cctcgccgcc
     9001 gatctcgtcg tacaggctca tggcttccct ccggtcgggc cgatgaacgg tgcggccggc
     9061 ggccccatga ccccggcgga gtcaagggca ttcttcagcc gctcccgcag ttcgcgctgc
     9121 acggcgaact gctcgccggg agcgcacgtg atgacggtgc gcacggtcat cgtcgtgccg
     9181 atgatcgact cgaccccgag cacggtcggc ggttcgatga gctgctcctg ccagtcgggg
     9241 tcggccccca gttccgctgc ggcttcgcgg atgatgccga cgacgcggtc gacgttctcg
     9301 gcgtaggaga ccggcatgtc caccgtggcg gtggcaaccc cctgcgaacg gttgccgatg
     9361 cggatgatct cgccgttgcg cacgtaccac gtgaccccgt tggcgtcccg gagccgggtg
     9421 atgcgcaggg tgacctcctc cacggtcccg atggcctcgc cggtgtcgat gacgtcgccc
     9481 acgccgtact ggtcctcgat gatcatgaac accccggaca ggaagtcctt gaccaggctc
     9541 tgcgccccga aaccgagcgc gacacccgcg acacccgccg aggcgagcaa cggcccgagg
     9601 gggatgccca gcagcgccat gatcgtgagg atggccagcg tcgcgacggt cagggtcacg
     9661 acgctgcgca gcagcgagcc gacggtctcg acccgctggc gctggcgctc gttgtcgaga
     9721 ccggtcgcgc tggcgagcac tcgcccggcc cgtccggact cggccaggcg gcgggccgag
     9781 cgcgaggtca tcgtcgcgac cacccggtcg atgacccgcc gcagggccca cagcgccgtc
     9841 atggcgatga cgatggtgag caggatgcgg gcaccgtcgg tgagcagcca ctgctgggtc
     9901 tgctcccacg tcgggagcac gggcgctacg gggtccacgg tgcgtcaggc tcccgcgtcg
     9961 cgggcctggg cggccagggc ccgggccacg gggtcacggt tctcgacgac gagccggcgc
    10021 agcgcggccg gggcatcggg gtgggtgtcg agccaggcct gggtggcgtc gtgcaggtcg
    10081 cggtcgacga ggggacgcgg gtagaacccg tagaccaccg attccaccag ggcgtgcgac
    10141 ccgcgctcct cgacggtgtc gagcatgcag tggtagcgct cgacgtaggg gcgcagcagg
    10201 tcggcgggtg tgcccgggcg gctgaacccc tgaccgatgg cgtcgacgac ggcgttcggc
    10261 aggccggagc cctcgacggc cgcggcccag gcggcttcct tcgcctgcac cgtggggcgg
    10321 gcggccttcg cgcgagcggc gcgctcgcga ccggtggcgg tgtcctcact ggccagctcc
    10381 gcgtcgatct gcgcgtcgtc ggcctcaccg gcggccgcga gggcggtgag cagggtccag
    10441 cgcatctcga agtcgacctt gagcccgtcg aggacctcgg tcccgtcgag cagggcgcgc
    10501 accgatgcga cgtcgtcacc ggctgtcgtc agcgcacagg cggcgctcac gagctggagc
    10561 tgtgcgtcgg tgccgggctc gctggcccgg gcgatctccc acagccggtc ccgcgtcgag
    10621 gcgcgggtgg cctcgcgctg ctcggggtcg gtgtaggaga gcaccgaggt ctgcagttgg
    10681 gcgatgaggg tgcgcagcag ggtcgagtcg gtctcaccgg gcagcgtcgc cagcacgacg
    10741 tcgacgaacg cgcgagcacc catctcggcg tcgcgggtca tgtcccacag gcaggccagg
    10801 accagcgagc gggggagcga ggaggtgaac ccgcgcgggt gggtgagggc cgtggccagc
    10861 gagcgctcgt cgaggcggat cttcgcgtag gccaggtcgt cgtcgttgac caggagcagg
    10921 tcgggctgct cgcggccgac gagctcggcg acctcggtga ccgcaccgtc gacgtcgacg
    10981 gcgatgacgt cggtgcggtg cagcgcaccg tcgcgaacgg catacagccc gacgttgagc
    11041 gtgtgcgggc gcaccgtctc gaagccggga gcgcaggtct gctcgatcac cgccgaggcg
    11101 atgagcccac ggtcgtcgac ctccaggacg gggcgcaggg tgctgacccc ggcggtctcg
    11161 agccacagcc gtgaccaggc gcgcaggtcg cggcccgagg tcgcctcgag ctcgacgagc
    11221 aggtcgtcga gggtcgtgtt gccccatgcg tgcttggcga agtaggcccg cagaccgtcg
    11281 cggaacggct cgcggccgac gtgggcgacg agctgcttga gcaccgaggc gcccttggcg
    11341 taggtgatgc cgtcgaagtt gacctcgacg tcgcgcaggt cacggatctc ggcgacgatc
    11401 gggtgggtgg tcgagagctg gtcctggcgg taggcccagt ccttctcgtg ggtgccgaag
    11461 gtcgtccagg cgtcggtcca ggcggtggcc tcggcctggc aggtggtcga ggcccactcg
    11521 gcgaacgact cgttgagcca gaggtcgttc caccagcgca tcgtcacgag gttgccgaac
    11581 cacatgtggg cgagttcgtg cagcagggtc agggcgcgcc gctcgaccag cgcctcggtg
    11641 acgcgggagc ggaagacgta gacctcgttg aaggtcaccg ccccggcgtt ctccatcgcc
    11701 cccatgttgt actcgggcgt gaagatctgg tcgtacttct cgaacgggta ggcacagtcg
    11761 aactcggcct cgaagaaggc gaagccgcgc ttggtgaggt cgaagaggtt gtcggcgtcg
    11821 aggtagggcg tcagcgactt gcggcagaag atgccgaggg gaacctcgcc ccgacggctg
    11881 gtcagggtgt cgcgcacgac gtcgtagggc ccggcgacga gtgcggtgat gtagctcgag
    11941 atgcgcgggg tgggggagaa ccgccacgtg gcgacgccct cgccggtggg ttcggcgtcg
    12001 ggggtggggg agttggagac gacctgccag tgcgccggcg ccgtcaccgt gaaggtgaag
    12061 gcggccttga ggtcgggctg ctcgaacacc gggtacatgc gccggctgtc gggaacctcg
    12121 aactggctgt agaggtagac ctcgtcgtca accgggtcca cgaagcggtg caggccctca
    12181 ccggtgttgg tgtagcgacc cgtggcggtg atcacgacga ggttgtcggc ggcgaggcgg
    12241 ggcagggtga tccggctgtc ggcgcagtgc gcggccgggt cgagctcggt gccgttcagg
    12301 gtgaggccgg tgaccgcgtc acccacgaag tcgacgaagg tctcgctacc cggctcggcg
    12361 caggcgaagc ggatcgtgct tcgcgtggcg aaggtctcgg ggccggtcgt gacgtcgagc
    12421 tcgacctcgt gggtgtcgac ggtgacgagg gccgcacggg ctgcggcctc ctcacgggtg
    12481 aggttggtgc cgggcacggg ttctccttgt gtgggcgtgc aggtgggcgt ggtggtgttc
    12541 acgcctgacg aacggtcgga tgccggcggt cgtctcgggc ggccggtccg ggtcgtcagc
    12601 catcctgtca cggggcgctg acgtcgcgtg gtcgctctgg tgtgcccatc gggctcgggc
    12661 cgtgggacgg gtgggtgttc tcgtcggtgt cgggcccggg tggcaggatg tgcgggtgcc
    12721 tgacaccact gagaagaccg ccctgaccac gtccgcagag cgcgcgagcg ccgagttctg
    12781 gttcgacccg ctctgcccgt gggcctggat gtcctcgcgc tggctcggtg aggtgcagca
    12841 ggtgcgcccc gtcgacgtca cctggtcggt gatgagcctg gcggtgctca acgagggccg
    12901 tgacctcccg ccgcagtatg ccgagctcat ggagcagggc tggggcccgg ttcgggtcgt
    12961 catcgcggcc cgcgaggcgc acggcgagca ggtcgtcaag ccgctctacg acgccctcgg
    13021 cgagcacatc caccaccgcg aggaggacta ctccacggcg atcgcgggtg cgctggctga
    13081 tgtgggtctg cccgcggagc tggccgaggc ggccacctcc gacgcactcg atgacgtcct
    13141 gcgcgccagc caccagcggg ccatcgacct cgtcggtgac gaggtcggga ccccggtgat
    13201 cgccgtcgac ggcgtggcgt tcttcggccc ggtcgtctcg cccgctcctc gcggcgaggc
    13261 cgcgggtcgc ctctgggacg ggtgcgtcct cgtcgccggc actgacggtt tcttcgagct
    13321 caagcgttcg cgcacccgcg agcccatctt ccacacggag gcctgacatg cgcgttcacc
    13381 tcggcgggga ccacgccgcc cacgacctgc tcctcgacct cgtcgcgttc ctccgggagc
    13441 aggggcacga cgtcaccaac cacggccccc acgagtacga cgccctcgac gactaccccg
    13501 tgttcgtcct gcgcgccgcg gaggccgtgg ccgcggaccc cggctcgttc ggtgtcgtgc
    13561 tcgggggctc cggcaacggc gagcagatgg cggccaacaa ggtcaccggc atccgtgcgg
    13621 ccctgtgcta caacgacgag ctggcctcgc tcgcccgtga gcacaacgac gcccaggtca
    13681 tctccatcgg cgcccggatg aacaccgtcg agcaggcccg ttcgatggtg cagaccttcc
    13741 tcgcgacccc attcagtggc gacccgcgcc acgtgcgacg gatcacgatg gtcgccgact
    13801 acgacgagtc cgggacgctc ccgccgctgc cttccgcgtg atcggtgggc gcgccacacc
    13861 acgtctggcg gtctgaggcg agcaccggca tccttgctga ggatgcccca cagcaaatgg
    13921 tcgcaatttt aaccttttgc cggttagggt gtgcccgctg gtctgaccgg aaggggtgcg
    13981 atgcccaatg acgtgatcgt gcgtccgcga ccgcgtgcgt accccgcgat gcgatggctg
    14041 ggcggatcac gacgcgccgg gtcggtctcc gagcgcgccc ttctcgtgtc gttcgtcggg
    14101 gggttgttgg ccttgggtgc cgccatcgcg cggtggccgc acgccgtgcc gatctcgttc
    14161 ctcttcccca cggtcgtcgt cgccggtgtg gtcctgcgcc cccgtcccct ggtgatggtc
    14221 tatggcatcg cgctcgtcct cgtcctcggc tgggtgcccc actccggcgt cgggctgacc
    14281 cgctcgatgt tcgtggcggt ctcggtcgga gccgtgatgg ccgtcatgct catcgcgtcg
    14341 gtgtcgcgcg cgcggatcgg gacccgaggc tttcgcggcg accgcatgtt cgccgaactg
    14401 cgcgatcgca tcgccctcgg cggccagctg cccgatctgc ccccgggctg gcaggtgtcg
    14461 tcgtgcatcc gctcggccca cggcgagcac ttctccggcg acttcctcgt cgcccaccga
    14521 tcgccctcgg gtgatcgcct ggagatcgtg ctcgtcgacg tcagcggcaa gggcacccgc
    14581 gccggcaccc gctcgctgct gctgtccgga gccttcggcg gcctgctcgg ggcggtgccc
    14641 tcggaccagt tcctggccac cgccaacgac tacctcgcgc gacagggctg ggtcgagggc
    14701 ttcgccacgg ccgtgcacct cgaggtcgac ctgcgcacgg gggagtacgc gatcggcaac
    14761 gcagggcatc cctcggcggc gctcttcacc ttcggcaagg gccgctggga ggtcatcgcc
    14821 ggtggtcggg gtccactcct cggtgtggtc cccggcgctc acttcccgcg ccagcgaggc
    14881 tggctcggcc acggtgacac cctgctgctc tacaccgacg gcatcgtcga ggcccgagga
    14941 cgcgacctcg tcgacggcgt cgaccgcatg ctcggcctcg ccacgacgac gcttctgagc
    15001 cagggcgacg tcgcgcgcga agtgtgcagc gctgcccgat cgggggagga tgacgaccgg
    15061 gcggcactgg ccgtccgccg tctgtgacca cgcgtgagag gaccccctga gtgggagccg
    15121 tgcccgagac cgagccgtcg atcgatgctg ccgctgaggc cgacccgcta cccgcgccac
    15181 cgaacggcat ccgcacgttc accccgcgct ggcgcaacag cccactcacc gacgagcgga
    15241 tggcccggct catgcccgca cgggcactgc ccgagggtcc gctcgtgccc gaggtggcct
    15301 tcgggcgcac gggcccggtc gtcgtcgaga tcggctccgg gcacggggcc gcggccatcg
    15361 cgttctgtgc cgcccacccg gaggccgacc tcatcgccgt cgaggtgcac gtgcccgggg
    15421 tggcccggat gctggcgctc gccgaggacg ccggggtcga gaacctctgg gtgcacccgg
    15481 gtgatgccct gcccttcctg accgaccggg tggcgccggg ggcgctcgcg gccgttcacc
    15541 tgttcttccc cgatccctgg cccaagaaca agcacgccaa gcgtcggttc gtgcagcagc
    15601 acaccctggc gctcatcgcc tccaggctcg aggtcggtgg gcagctgctc atcgcgaccg
    15661 acatcgactc ctacgcggcg cactcgcgcg cccagctggc cgagcacggg cgctgggacg
    15721 tcgtcgaggg cgtgcgcccg ccgtggcggc cggatgccgg gttcgaggcc aagggggtgc
    15781 gggccgggcg ggtcgtcagc gagctccggg caacgctgcg gccgctctga gcggcctcac
    15841 gcccgctgcg aacgcagaag ttcaggtctc gaccggtaat tgctgcgtgc gatccgaact
    15901 tctgcgttgc tgggtccgag ccgaacgcag aagttcgggt ctcgaccggt aattgctgcg
    15961 tgcgatccga acttctgcgt tgcaggccgt gcgttgctgg tcggggtagc cggattcgaa
    16021 ccggcggcct tccgctccca aagcggacgc gctaccaagc tgcgccatac cccgcagccc
    16081 acggacggca tccgagaacc tcgggccaga agtcagccat gaggtgagcc tcggatttcg
    16141 gatcccgcga gcgcggccac taggctagcc gctgcactcg gggtccttga ccacgaggtg
    16201 tgcgggcgta gctcaatggt agagccccag tcttccaaac tggctacgcg agttc
//
LOCUS       DAOSOC010000041        16020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3684_length_16020_cov_8.417288, whole genome shotgun sequence.
ACCESSION   DAOSOC010000041 DAOSOC010000000
VERSION     DAOSOC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 16020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16020
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3684_length_16020_cov_8.417288"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(418..750)
                     /locus_tag="PL014_05070"
     CDS             complement(418..750)
                     /locus_tag="PL014_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT56494.1"
                     /translation="MVRFRVLGPVDVFVDGAPVRLRPQERRLLALLLCRRNRPVPTED
                     LVDELWNGSPPATARTAIRVHVERTRAEGVEVPEDQRTLTSSAHEDLDDARAHGVLPR
                     ARRLLHAL"
     gene            complement(849..2213)
                     /locus_tag="PL014_05075"
     CDS             complement(849..2213)
                     /locus_tag="PL014_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT56495.1"
                     /translation="MSTLAFLLGVLFMATGIAASIALHEVGHLVPAKRFGVRVTQYMV
                     GFGPTMWSRRRGETEYGVKAIPLGGYIRMIGMFPPRKGDDPGTVRVSSTGRFSQLVDE
                     ARAASLEEVRPGDENRVFYRLSVPKKVIVMLGGPTMNLLIGVFLLTLLVTVHGVATAQ
                     PGAVVASVSQCVVPVTEAGDATTCTNQPDTPALAAGILPGDLILSIDGQPIDDTRDIS
                     TLIRPRVDQAVTIVLERDGEQLTVTATPIRNVVPVLDADGVPVTTADGTEQTTEAGFL
                     GVRTTAPVEYVQQPVTAVPGIIGTYVSRTAEAIVHIPSRMVGVWQAAFGGAEREIDSP
                     MSVVGVGRVAGEATEGKYDAFVGEGVGNKIWFLVGLLAALNFMLFIFNLIPLLPLDGG
                     HVAGALWEGLKRQIAKLRGRPDPGYVDVAKALPVAYTVSMVLLGFTALLIYADLVKPV
                     NLGG"
     gene            complement(2210..3430)
                     /gene="dxr"
                     /locus_tag="PL014_05080"
     CDS             complement(2210..3430)
                     /gene="dxr"
                     /locus_tag="PL014_05080"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOT56496.1"
                     /translation="MVSVTSRSVAVLGSTGSIGTQALEVIAAHPGAFTVAALTAGGNV
                     ELLARQAVEFDVPLVGVARGQVEDVRAAIAAAAADAGRPASVTDVVVGADAATIAAGC
                     GADVVLNGITGSIGLRPTLAALEAGSVLALANKESLIIGGPLVKAAAAPDQIVPVDSE
                     HSAIAQSLRGGRADEVRRLVVTASGGPFRGRSRAELADVTPQQALAHPNFSMGRVITT
                     NSATLVNKGLEVIEAHLLFDIPFDRIDVVVHPQQQIHSMVEFHDGSTIAQLGPPRMLV
                     PIALGLSWPQRLTDVDVPCDWSLAQSWEFHPLDDDAFPAVRLARRVGEAGSTFPAVYN
                     AANEVCVDAFHDGVISFTDIVDTVERVVEAHTGSELDASSVEGVLAADRWARHRAADL
                     IGIAVDDDQIQENR"
     gene            complement(3433..3780)
                     /locus_tag="PL014_05085"
     CDS             complement(3433..3780)
                     /locus_tag="PL014_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HOT56497.1"
                     /translation="MIRHLRPPVDVDALVANAVARGRVVRLVVAGETKSATLAAFADA
                     LDFPGWFGGNLDALADLLEHLARHEDGEAELVVDGVLALQRDDPFAAAQLNGVLGEVA
                     LAHPRFHVCVIER"
     gene            complement(3863..4927)
                     /locus_tag="PL014_05090"
     CDS             complement(3863..4927)
                     /locus_tag="PL014_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOT56498.1"
                     /translation="MSHSPDAAAPATRSARHVDRRRSVKDLLDEADASLRRGARPGAT
                     VWPTGFDLLDATLDGGLRSGELVLLGGSEGSGKTTIALQMVRNAVRTGRHAVVFSFEH
                     EAGTLIQRLIALEASCLAVEAGQHPAAAADVHAVRGVFEAPDPDRRGLAEALAGIPYG
                     ADALAAVLDYAPRLHIHESTSDTTPAEVARVLAEVREEADEPPMVMVDYLQKVPLDGA
                     AGDEVARVTVVTETLKDMALELECPVVCISAADRESLGAGHRMRTRDLRGSSALAYEA
                     DLVLILSSKENIVSREHLVYDLGSVQRFRRWAVITVEKNRHGLGHVELEVEKDFEHGR
                     FHPQARVVTERLIEERIFTT"
     gene            complement(4984..6249)
                     /locus_tag="PL014_05095"
     CDS             complement(4984..6249)
                     /locus_tag="PL014_05095"
                     /inference="COORDINATES: protein motif:HMM:NF039738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6357 family protein"
                     /protein_id="HOT56499.1"
                     /translation="MRDVVFTRQGWFPAVVRGESGLSIELAAGADANHDPRVFRFPVD
                     EVRAAVIEADLRRHLLLWSAVLPLCTDAGSHGDLDVEAAVALLDPTLLGTPDEVDALF
                     RGIRWDWRLLMAHGGDIDLLDDGKVWAALSEATVTPDRRRVEEYEANRRRAQRGVTLA
                     PLDTAILRFTGQYVHGATLPKRLPDEVDPALLPDVLRVIATAERATAGMRIGPDPRHG
                     RGAADKAAWERVATAAEEAVRAAHPELADDAVATVRFLMCSEVRARSKNLPEEDDAEG
                     VGDRAGGPGGVRTAGLTFTDDKEVAQPWAPGDSRTAGAAFWEFVASRSAPDNEVFTIE
                     DEQLVEGLQLHFYADSVARITTVTRGEGASEPEYRVEYTLVDGLDGFRTRVRAFVDGG
                     CAALDPHGPWTADLAELERARTRRREANS"
     gene            complement(6285..7745)
                     /locus_tag="PL014_05100"
     CDS             complement(6285..7745)
                     /locus_tag="PL014_05100"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOT56500.1"
                     /translation="MAFTQKSLVESVPTRLLIGGSWREASGGARFEVSDPASGAVLTT
                     CADGTPEDGLAALAAAHEAQPTWAATPPRERSEILRRAFDALVARTDEFATLMSLEMG
                     KTVAEARGEVAYGAEFFRWFAEEAVRIHGRYSVAPNGASRLLTMKQPVGPTLMITPWN
                     FPLAMGTRKIGPAVAAGCTMVVKPAHETPLTMLLLAEVMMEAGLPDGVLNVVTTTHSG
                     AVCEPLIRDERLRKLTFTGSTPVGKMLVEQSAEQLLRLSMELGGNAPFLVFEDADVDA
                     AVDGAMLAKMRNIGEACTAANRFFVHESLAEEFATKLAARMGALVVGKGTKKGVDVGP
                     LITGKARDKVDELVQDAVGKGARVLTGGAPVPGKGFFYAPTVLADVPADSRCLGEEIF
                     GPVAPVCTFTDDADAVAKANGTEYGLVGYIYTNDQNRMIRVAEAMEFGMIGVNTGIVS
                     NPAAPFGGVKQSGFGREGGFEGIDEYLETKYVGIAL"
     gene            complement(7814..9097)
                     /locus_tag="PL014_05105"
     CDS             complement(7814..9097)
                     /locus_tag="PL014_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOT56501.1"
                     /translation="MRVLMVGAGGVGDAAARIAAERSFFEQWVVADYDLTRSERTVAS
                     VIERNPGDTRFRAARVDASDAGAVEALAREVGATHVFNAVDPRFVLPIFEGARAAGAD
                     YLDMAMSLSSRHPEQPYAKVGVKLGDDQLAQAEQWEADGRLALVGIGVEPGLSDVFAR
                     YAADHLFSHIDELGTRDGANLVIRDEDGNEVFAPGFSMWTIIEECLNPPVVWEKARAV
                     GSDGGYDVEAGFFTLPPFSEPEVFDFPEGIGPVECVHVEHEEVLLMPRWVDAEKVTFK
                     YGLGEEMITILRTLHTLGLDSTDPVTVKGVQVSPRDVVAAVLPDPATIGPRMEGKTCA
                     GLWVTGTGKDGMPRRTYLYHVSDNADTMRDYGAQCVVWQTAINPVVALELLANGTWSG
                     AGVLGPEAFDAVPFLDLLAAPKPQGYGSPWGLEDR"
     gene            complement(9189..9962)
                     /locus_tag="PL014_05110"
     CDS             complement(9189..9962)
                     /locus_tag="PL014_05110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56502.1"
                     /translation="MITALLVLFYLFLPVAYTFAFSFNNYKKSNISWNPEGSPTLKHW
                     KDPCGAPGVCESLVTSIQIGVIATVIATVLGTMLAFAMVRHRFRGRGTSNVLVFVPMA
                     TPEIVLGASLLTIFVQGFSNVGLQLGFWTIVIAHIMFCVSFVVVTVKARLQSLDPRLE
                     EAAQDLYAGPGSTFWKITFPLVLPGIVGAALLAFSLSFDDFIITNFVSGNETTFPKFV
                     YISYLRGIPAQANVIGFSMFVIALALVIGGQLIGNARKK"
     gene            complement(9989..10903)
                     /locus_tag="PL014_05115"
     CDS             complement(9989..10903)
                     /locus_tag="PL014_05115"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56503.1"
                     /translation="MSALAHVSSAGGTTPPPAPQPNRKGRSWVPYLLLLPGALWLLVF
                     FVIPLGQLFSVSLQSQFPGYPGYYYRDLNFGNYVTALTDFAPHFGRSILFATLATFFA
                     FCLAYPLAYAMAFKAGRWRNVMLIAVIAPFFTSFILRTVAWRQILADEGPVASTLNTL
                     HLLPGGRITETWIAVVAGLTYNFLPFMVLPIYASLERADSRVIEAGGDLYANGFTTFR
                     TVTLPISMPGVLAGTLLTFIPAAGDYVNAELLGSDNTKMIGNVIESQFFRVPGGFPTA
                     AALSFTLMALILAMVFVYVRRFGTEELV"
     gene            complement(10900..12048)
                     /locus_tag="PL014_05120"
     CDS             complement(10900..12048)
                     /locus_tag="PL014_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56504.1"
                     /translation="MARKNASGFRDAQGDLRLENVTKSFAEFTAVDDLSLVVPRGSFF
                     ALLGPSGCGKTTTLRMVAGLEQPTSGRLLIGDTDLTGSRSYERPVNTVFQSYALFPHL
                     TIRDNVAFGPKRRGQADAAALAEDALKLVQMDHLAARKPAQLSGGQQQRVALARAIVN
                     RPEVLLLDEPLGALDLKLRRQMQVELKRIQTEVGLTFIHVTHDQEEAMTMADTVAVMN
                     HGRIEQMGPPAQMYDLPRTAFVANFVGQSNLGSGRVIDRDGTHLVAEIQGTKVKIPIA
                     RSAVTDGDITFGVRPEKVRVMRRPSEGAGDDVKGIVLDVSFTGVATQYLVQLPSGTVW
                     SCYEQNLDVEPIDLRPGDDVWLSWNPHHAFGVPVDEAEARAAAEAGLT"
     gene            complement(12048..13286)
                     /locus_tag="PL014_05125"
     CDS             complement(12048..13286)
                     /locus_tag="PL014_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT56505.1"
                     /translation="MALRRRPPADPMTRGLISAARSSALTRRSLFGTAALGGAAAALS
                     ACAPPPPPSGGVASLVLPEDLSDTDKVVRWANWTAYLDYDEETKKYPTLERFIAETGI
                     RAEYTEDIEDNDAYFNKIAPQLRAGQDIGRDIFTFTDWMANRIIRDQLCQPLELITMP
                     NAPNLLPALQNVSFDPGRNFSLTWQSGFAGIGYDKSKVSKEIKTLDDLWTDELKGKIV
                     VLSEFRDTVGIIMQSQGVDITSEFGKAEFEAAVAEIEKRMDEGYIRRIKGNSYLEDLK
                     SGNAVAGIVWSGDLFILRAETENENWEFVIPESGGTIWSDNMMVPITSQHRRNAQTLM
                     NFYYDPEVAAEVAAWVNYVCPVVGAREVLAKTDPELAESPFIFPSEAFIADNNIQGFR
                     ALSPEEDQEYSAIWQKVMGN"
     gene            complement(13299..>13814)
                     /locus_tag="PL014_05130"
     CDS             complement(13299..>13814)
                     /locus_tag="PL014_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT56506.1"
                     /translation="SAPVGLPDDEDALFGPVNNSRQLAHVSGFIDRLPSHATVSAGGN
                     RRTDLGEGYYLEPTVLSGLRQDDEAIQNEIFGPVITVQKFTDEAEAIRWANGVDYGLA
                     SSVWTKDFGRAMRMSKALDFGCVWINTHIPLVAEMPHGGYKKSGYGKDLSMYGFEDYT
                     RIKHVMANIES"
     assembly_gap    13815..13914
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13915..14576)
                     /locus_tag="PL014_05135"
     CDS             complement(<13915..14576)
                     /locus_tag="PL014_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT56507.1"
                     /translation="MSGQRHLRNFIGGEYVDGRTDSTTEIINPATGSVVATAPVSGQE
                     DVDAAYAAAEQAFAGEWGATTPSDRQQALLKFADAVEAHADELIALESENTGKIKALT
                     ASEEIPPMVDQLRFFAGAARVLEGRASAEYMAGHTSWIRREPIGVVGQVTPWNYPLLM
                     AVWKIGPALAAGNSVVLKPSDTTPESTLRLAELASQFLPAGTLNVITGDRETGRMLVE
                     HRT"
     gene            14817..15257
                     /locus_tag="PL014_05140"
     CDS             14817..15257
                     /locus_tag="PL014_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT56508.1"
                     /translation="MDDIAKHIIELLQEDGRQSYAAIAKAVGLSEAAVRQRVQRLLDA
                     DVMQIVAVTDPLQVGFSRQAMIGIRVSGDLLAVGDALTAMPEVDYVVTTAGSFDILAE
                     VVCEDDDHLLDLLNQQIRTLPGVTSTETFVYLKLNKQHYNWGTR"
     gene            15254..>16020
                     /locus_tag="PL014_05145"
     CDS             15254..>16020
                     /locus_tag="PL014_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT56509.1"
                     /translation="MSTYPSDVWEPSSDRHTATGALYSDAARDHLWMHFTRHSTFEGH
                     GLGHSVPIITKGQGWTIWDDRGNEYIDALAGLFVTNVGHGRTEIAEAMAKQASELAFF
                     PLWSYAHPRAIELAERLAHHAPGDLNRVFFTTGGGEAVETAWKLAKQYFKLTGKPTKH
                     KVISRSIAYHGTPQGALSITGIPAAKSMFEPLVPGGHKVPNTNIYRAPEHLRDDEKAF
                     GRWAADRIAEAIEFEGPETVAAVFLEPVQNSGGCFPPP"
BASE COUNT         2455 a         5399 c         5640 g         2426 t
ORIGIN      
        1 gattgcccac agggttgctc cacggtctgt cgccagcctg tcgcagacct gacactgcgc
       61 tccaccgcac cgcgcgttca ctggtttcca ggtcgaaaac tgccgtgtcg atcgcatcca
      121 gtcatgcggc aattccccgt tgtcgcgatg cagcgaagac tgcatggtct cgtaacgacc
      181 acgtcgcaga gatcggccct ttcggtgaag tggtggaaat cccccgagcg gtcggaaggc
      241 gctatctccg ctatgcggta aacgaagaac gctcttatgc accctgcctt ggggcgggac
      301 gttatggagg cttccgtccg ggcctgtgca tcgagctcgg tatcgcgtgc tgggcaggcg
      361 tgcgatctgc ccgaatccgc gcttgatcaa tgcagcccag gcaggccaac cagcagatca
      421 caacgcgtgc agtagccgcc gcgcacgcgg cagcacaccg tgggctcggg catcgtcgag
      481 gtcctcgtga gcagaagatg tgagagttct ctgatcctcg gggacctcga ccccttcggc
      541 cctcgtgcgc tcgacgtgga cccggatcgc ggtgcgggcg gtcgcgggag gactgccgtt
      601 ccacagctcg tcaaccaggt cctcggtcgg gaccggacgg ttccggcggc agagcaacag
      661 cgcgagcagc cgtcgctcct gtggccgcag ccggacgggg gcgccatcga cgaacacgtc
      721 gaccggcccg aggacccgga aacggaccat gagcccacct cctgcccggg tcgatccgcc
      781 gacgcttcgc tcagggtagc gaggcaccac cgaggcagat ggtcatcgac ggcgtcccgc
      841 cccaggcgtc agccgccgag gttgaccggc ttgacgaggt cggcgtagat gagcagcgcc
      901 gtgaagccga gcagcaccat cgacacggtg tacgcgacgg gcagcgcctt cgcgacgtcg
      961 acgtagccgg ggtccggccg cccgcgcagc ttcgcgatct ggcgcttgag cccctcccac
     1021 agggcccccg cgacgtgacc accgtcgagg gggagcagcg ggatgaggtt gaagatgaag
     1081 agcatgaagt tcagcgcggc gagcaggccg acgaggaacc agatcttgtt ccccacccct
     1141 tcaccgacga acgcgtcgta cttgccctcg gtggcctccc ccgcgacccg gcccacgccg
     1201 acgacggaca tcgggctgtc gatctctcgc tcggccccgc cgaacgcggc ctgccagacc
     1261 ccgaccatcc gcgacgggat gtggacgatg gcttcggcgg tgcggctcac gtaggtgccg
     1321 atgatgccgg gcacggccgt gaccggctgc tgcacgtact cgacgggggc ggtcgtgcgc
     1381 acgccgagga agccggcctc ggtggtctgc tcggtgccgt ccgcggtggt gaccggcaca
     1441 ccgtcggcgt cgaggaccgg cacgacgttg cggatcggcg tcgccgtgac ggtcagctgc
     1501 tcgccgtcgc gctcgaggac gatcgtcact gcctggtcga cgcgcgggcg gatcagggtg
     1561 ctgatgtccc gggtgtcgtc gatgggctgg ccgtcgatgg acaggatcag gtcgccgggc
     1621 aggatgccgg ccgcgagcgc cggcgtgtcg ggctggttcg tgcaggtggt cgcgtccccc
     1681 gcctcggtca ccgggacgac gcactgcgac accgacgcga cgaccgcacc gggctgggcc
     1741 gtggccaccc cgtgcacggt gacgagcagg gtcagcagga agacaccgat gagcaggttc
     1801 atggtcgggc cgccgagcat gacgatgacc ttcttgggca ccgacagccg gtagaagacc
     1861 cggttctcgt cgccgggacg cacctcctcc aggctggccg cgcgcgcctc gtcgacgagc
     1921 tggctgaacc ggcccgtgct cgacacgcgc acggtgccgg ggtcgtcgcc cttgcgcggg
     1981 gggaacatgc cgatcatccg gatgtaccca cccagcggga tcgccttgac cccgtactcc
     2041 gtctcgccgc gacggcgtga ccacatcgtc gggccgaagc cgaccatgta ctgcgtcacg
     2101 cggacgccga agcgcttggc cggcacgagg tgccccacct cgtgcagggc gatcgaggcg
     2161 gcgatcccgg tggccatgaa gagcacaccg agaaggaagg cgagcgtgct caacggttct
     2221 cctggatctg gtcgtcgtcg acggcgatgc cgatgaggtc ggcggcgcgg tgacgggccc
     2281 accggtcggc ggcgagcact ccctcaacgc tcgaggcgtc gagttcgctc ccagtgtgcg
     2341 cctcgacgac gcgttccacc gtgtcgacga tgtcggtgaa cgagatcacc ccgtcgtgga
     2401 aggcgtcgac gcacacctcg ttggcggcgt tgtacacggc cgggaaggtg ctgccggcct
     2461 cgccgacccg gcgggcgagg cgcacggcgg ggaaggcgtc gtcgtcgagc gggtggaact
     2521 cccagctctg ggccaggctc cagtcgcacg gcacgtcgac gtcggtcagc cgctgcggcc
     2581 aggacagccc gagcgcgatc ggcacgagca tgcgcggcgg gccgagctgg gcgatggtcg
     2641 agccgtcgtg gaactcgacc atcgagtgga tctgctgctg cgggtgcacg acgacgtcga
     2701 tgcggtcgaa ggggatgtcg aagagcaggt gggcctcgat gacctccagc cccttgttga
     2761 ccagcgtcgc ggagttcgtc gtgatgaccc gccccatcga gaagttgggg tgggccagcg
     2821 cctgctgcgg ggtgacgtcg gcgagctcgg cgcggctgcg cccgcggaac gggccaccgc
     2881 tggccgtcac gacgaggcgg cgcacctcgt cggcgcgccc gccgcgaagg ctctgcgcga
     2941 tggcgctgtg ctcggagtcg accggcacga tctggtcggg cgcggcggcc gccttgacga
     3001 gcggcccccc gatgatcagc gactccttgt tcgccagcgc cagcaccgag cccgcctcga
     3061 gggctgccag ggtcggccgc agaccgatcg acccggtgat gccgttgagc acgacgtcgg
     3121 cgccacaccc cgcggcgatc gtcgcggcat ccgcgccgac cacgacatcg gtcacggatg
     3181 ccggccggcc ggcatccgct gccgccgcgg cgatcgcggc gcgaacgtcc tcaacctggc
     3241 cgcgtgcgac accgacgagc ggcacgtcga actcgacggc ctgacgtgcg agcagctcga
     3301 cgttgccccc ggcggtgagg gcggccacgg tgaaggcacc cgggtgcgcg gcgatgacct
     3361 cgagggcctg ggtgccgatc gagccggtgg agccgaggac ggcgacggag cgggaagtca
     3421 cggaaaccat tgtcagcgct cgatgacgca cacgtgaaac cggggatggg ctagggccac
     3481 ctcaccgagc accccgttca gctgtgccgc cgcgaacggg tcgtcgcgct ggagggccag
     3541 gacgccgtcg acgacgagct cagcctcccc gtcctcgtgc ctggcgaggt gctcgagcag
     3601 gtcggcgagg gcgtcgaggt tgccgccgaa ccacccgggg aagtccaggg cgtcggcgaa
     3661 ggccgcgagg gtggcggact tggtctcccc cgcgacgacg agacgcacga cgcgaccgcg
     3721 cgccacggca ttcgcgacga gggcgtcgac gtcgaccggc ggccggaggt ggcggatcac
     3781 acccccatcc tcccccgact tcttgcgctc cagccacctg ccgccccggc agatcgggtg
     3841 gctggcgtgc aagaagtcgc ggtcaggtgg tgaagatgcg ctcctcgatg agtcgttcgg
     3901 tgacgacccg cgcctgcggg tggaagcgcc cgtgctcgaa gtccttctcg acctcgagct
     3961 cgacgtgccc gagcccgtgc cggttcttct cgacggtgat gacggcccag cgccggaagc
     4021 gctgcacgct gccgaggtcg tagaccaggt gctcgcgcga gacgatgttc tccttgctcg
     4081 acaggatgag cacgaggtcg gcctcgtagg ccagggccga cgagccgcgc aggtcgcggg
     4141 tgcgcatccg gtgcccggca cccagactct cgcggtcggc cgcggagatg cacacgacgg
     4201 ggcactcgag ctcgagggcc atgtccttga gcgtctcggt gacgacggtg acccgcgcga
     4261 cctcatcgcc cgccgcgccg tcgagcggca ccttctggag gtagtcgacc atgaccatcg
     4321 ggggctcgtc ggcctcctcc cgcacctccg ccagcacccg ggccacctcg gcgggggtcg
     4381 tgtcgctcgt cgactcgtgg atgtgcagcc gtggtgcgta gtcgagcacg gccgcgaggg
     4441 cgtcggcgcc gtacgggatg ccggcgagcg cctcggcgag gccgcggcgg tcggggtcgg
     4501 gcgcctcgaa gacgccccgg acggcatgga cgtccgcggc tgccgccggg tgctggccgg
     4561 cctcgacggc aaggcacgac gcctcgaggg cgatgaggcg ctggatgagg gttcctgcct
     4621 cgtgctcgaa ggagaagacc accgcgtggc ggccggtgcg caccgcgttg cgcaccatct
     4681 ggagcgcgat cgtcgtcttg cccgacccct cgctgccccc gagcaggacc agttcgccgg
     4741 agcgcaggcc gccgtcgagc gtggcgtcga gcaggtcgaa cccggtgggc cagaccgtgg
     4801 ccccgggtcg cgctcctcgg cgcagcgacg cgtcggcctc gtcgagcagg tccttcaccg
     4861 agcggcggcg gtcgacgtgg cgggcgctac gggtggccgg ggcagcggca tccgggctgt
     4921 ggctcatgga ggacatgaca ccactgacgc gcggctgcgc gtgacgtttc ggcgggccac
     4981 gtcctaggag tttgcctcgc gacgccgcgt gcgagcgcgc tcgagctccg caaggtcggc
     5041 cgtccagggg ccgtgtggat cgagcgcggc acaaccgcca tcgacgaagg cgcgcacccg
     5101 tgtccgaaac ccgtcgagcc cgtcgacgag cgtgtactcg acccggtact cgggttccga
     5161 ggcgccctcg cccctcgtca ccgtcgtgat ccgggcgacc gagtccgcgt agaagtgcag
     5221 ctggagccct tcaaccagct gctcgtcctc gatcgtgaac acctcgttgt ccggggccga
     5281 acggctggcg acgaactccc agaaggcggc gccagcggtg cggctatcgc caggagccca
     5341 cggctgcgcc acctccttgt cgtcggtgaa cgtcagcccc gccgtcctca ccccgcccgg
     5401 accacctgca cggtcaccca ccccctcggc gtcgtcctcc tccggaaggt tcttcgaacg
     5461 agccctgacc tccgagcaca tgaggaatct cacggtggcc accgcgtcgt ccgccagttc
     5521 agggtgggcg gcacgcacgg cctcctcggc cgcggtcgcc acccgctccc atgctgcctt
     5581 gtccgcggcc ccccttccat ggcgtgggtc cgggccgatg cgcatccccg cggtcgcccg
     5641 ctccgccgtg gcgatcaccc gcagcacgtc gggcagcagt gcaggatcga cctcgtccgg
     5701 aaggcgtttc ggcagcgtcg cgccgtgcac gtactgtccg gtgaaccgaa ggatggcggt
     5761 gtcgagtggt gccaacgtga cgccacgttg cgcgcgccgc cggttcgcct cgtattcctc
     5821 gacccggcgc cggtcggggg tcacggtcgc ttctgacagg gccgcccaga ccttgccgtc
     5881 gtcgagcagg tcgatgtcgc cgccgtgggc catgagcagg cgccagtccc accggatgcc
     5941 ccggaacagg gcgtcgacct cgtcgggcgt gcccaggagg gtggggtcca gcagcgcgac
     6001 ggcagcctcc acgtcgagat cgccatggct cccggcgtcc gtgcacagcg gtagcacggc
     6061 actccacagc agcaggtgcc tcctcaggtc cgcctcgatc acagcggccc gcacctcgtc
     6121 gaccgggaat ctgaagaccc gaggatcatg gttggcgtcc gccccggcgg cgagctcgat
     6181 ggacagcccg ctctcgcccc ggaccacggc agggaaccag ccctgacggg tgaacacgac
     6241 gtcgcgcacg gatctgctcc tcgttcgctc cccgaccagc agcctcagag ggcgatgccg
     6301 acgtacttcg tctcgaggta ctcgtcgatg ccctcgaaac cgccctcgcg gccgaagccc
     6361 gactgcttga cgccaccgaa cggcgccgca gggttggaca cgataccggt gttgacgccg
     6421 atcatcccga actccatggc ctcggccacc cggatcatcc ggttctggtc gttggtgtag
     6481 atgtaaccga cgagtccgta ctcggtgccg ttcgccttgg ccacggcatc cgcgtcgtcg
     6541 gtgaacgtgc acaccggcgc gaccggcccg aagatctcct caccgaggca ccggctgtcg
     6601 gccggcacgt cggcgagcac ggtcggtgcg tagaagaacc ccttgcccgg cacgggcgcg
     6661 ccaccggtga gcacgcgggc ccccttgccg acggcgtcct ggacgagctc gtcgaccttg
     6721 tcgcgggcct tgccggtgat gagcgggccg acgtcgaccc ccttcttggt gcccttgccg
     6781 acgacgagcg cgcccatgcg ggccgcgagc ttggtcgcga actcctcggc gagcgactcg
     6841 tggacgaaga accggttcgc cgccgtgcag gcctcaccga tgttgcgcat cttcgcgagc
     6901 atcgccccgt cgacggccgc gtcgacgtcg gcgtcctcga agacgaggaa gggggcgttg
     6961 ccgccgagct ccatcgacag gcgcagcagc tgctcggccg actgctcgac gagcatcttg
     7021 ccgaccgggg tggagccggt gaaggtcagc ttgcgcagcc gctcgtcgcg gatgaggggc
     7081 tcgcacacgg caccggagtg ggtcgtggtg acgacgttga gcacaccgtc gggcaggccc
     7141 gcctccatca tgacctcggc gagcagcagc atcgtcagcg gcgtctcgtg ggcgggcttg
     7201 acgaccatcg tgcaccccgc ggcgacggcc gggccgatct tgcgggtgcc catcgcgagc
     7261 gggaagttcc acggcgtgat catcagcgtg ggcccgaccg gctgcttcat cgtcagcagt
     7321 cgcgaggcgc cgttgggagc caccgagtac cggccgtgga tgcgcacggc ctcctcggcg
     7381 aaccagcgga agaactccgc accgtacgca acctcaccgc gcgcctcggc cacggtcttg
     7441 cccatctcca ggctcatcag ggtggcgaac tcgtccgtgc gggcgacgag ggcgtcgaag
     7501 gcccggcgca ggatctcgct gcgctcgcgg ggcggggtcg ccgcccaggt cggctgggcc
     7561 tcgtgggcgg ctgccagcgc ggcgagcccg tcctccgggg ttccgtccgc acacgtcgtg
     7621 agaacggcgc cggatgccgg gtcgctcacc tcgaagcgcg cgccaccgct cgcttcccgc
     7681 caggaccccc cgatgagcag acgggtggga acggactcga cgagcgactt ctgggtgaac
     7741 gccatgggtg tgtccttctg ggtccgggcg gcggtgatcg gtggcggtac agcggtggtg
     7801 gcgagcctac gactcagcgg tcctcgaggc cccacgggga gccgtagccc tgcggcttcg
     7861 gcgcggcgag caggtcgagg aacggcacgg cgtcgaaggc ctccgggccg agcacgcccg
     7921 cgcccgacca ggtgccgttc gcgagcagct cgagggcgac gacggggttg atcgccgtct
     7981 gccagacgac gcactgggcg ccgtagtcgc gcatcgtgtc cgcgttgtcg gagacgtggt
     8041 agaggtaggt gcgccgcggc atcccgtcct tgccggtgcc ggtcacccac agtccggcgc
     8101 aggtcttgcc ctccatgcgc gggccgatgg tcgctgggtc gggcagcacg gcggcgacga
     8161 cgtcgcgggg gctgacctgc acccccttga ccgtgaccgg gtcggtgctg tcgagcccga
     8221 gggtgtgcag cgtgcgcagg atcgtgatca tctcctcgcc gaggccgtac ttgaaggtca
     8281 ccttctcggc gtcgacccag cgcggcatga ggagcacctc ctcgtgctcg acgtgcacgc
     8341 actccaccgg gccgatgccc tcggggaagt cgaagacctc gggctcgctg aacggcggca
     8401 gcgtgaagaa tccggcctcg acgtcgtagc cgccgtcgga gcccaccgct cgggccttct
     8461 cccacaccac gggcgggttc aggcactcct cgatgatcgt ccacatcgag aagcccgggg
     8521 cgaagacctc gttgccgtcc tcgtcgcgga tgacgaggtt ggcaccgtcg cgggtaccga
     8581 gctcgtcgat gtggctgaac aggtggtcgg cggcgtagcg cgcgaagacg tcggagagcc
     8641 cgggctcgac cccgatgccg accagggcca ggcgcccgtc ggcctcccac tgctcggcct
     8701 gggccagctg gtcgtcgccg agcttgacgc cgaccttggc gtacggctgc tcggggtgac
     8761 ggctcgacag gctcatcgcc atgtcgaggt agtccgcacc ggcggcgcgg gcgccctcga
     8821 agatcggcag cacgaagcgg gggtccacgg cgttgaacac gtgcgtggca cccacctcgc
     8881 gcgccagcgc ctcgaccgcg ccggcatccg acgcatcgac gcgggcggca cggaaccggg
     8941 tgtcccccgg gttgcgctcg atgaccgagg ccaccgtgcg ctcggagcgg gtgaggtcgt
     9001 agtcggcgac cacccactgc tcgaagaagg accgttcggc ggcgatgcgg gcggcggcgt
     9061 caccgacgcc gccggcaccg accatgagga cgcgcatggt gttcctgtct cagggtgtca
     9121 ccgggggtac cggtggtggt ggaggtccgg cccgccgggc tctcgcccgg cgggccggac
     9181 caggtcgttc acttcttgcg ggcgttgccg atcagctggc cgccgatgac cagcgccagg
     9241 gcgatgacga acatcgagaa accgatgacg ttggcctgag ccgggatgcc gcgcaggtag
     9301 gagatgtaga cgaacttcgg gaacgtcgtc tcgttgccgg agacgaagtt ggtgatgatg
     9361 aagtcgtcga aggacaacga gaacgcgagc agcgccgcac cgacgatgcc cgggagcacc
     9421 agcgggaagg tgatcttcca gaacgtgctg cccggcccgg cgtacaggtc ctgggcggcc
     9481 tcctcgaggc gcgggtccag gctctgcagg cgggccttga ccgtgacgac gacgaagctg
     9541 acgcagaaca tgatgtgggc gatgacgatc gtccagaacc ccagctgcag accgacgttg
     9601 gagaagccct ggacgaagat cgtcagcagg ctcgcgccga ggacgatctc gggtgtggcc
     9661 atcggcacga agaccaggac gttgctcgtg ccgcgcccgc ggaagcggtg gcgcaccatc
     9721 gcgaacgcga gcatcgtgcc gagcaccgtc gcgatgacgg tggcgatgac gccgatctgg
     9781 atcgacgtca cgagcgactc gcagacaccg ggggcaccgc aggggtcctt ccagtgcttc
     9841 agggtcgggc tcccctcggg gttccacgag atgttggact tcttgtagtt gttgaacgag
     9901 aaggcgaacg tgtaggcgac gggcaggaac aggtagaaca gcacgagcaa cgccgtgatc
     9961 atggcgaagc ggtttccgat ccagcggatc acaccagttc ctccgttccg aagcggcgca
    10021 cgtagacgaa caccatggcg aggatgaggg ccatcaacgt gaacgagagc gctgcggccg
    10081 tcgggaagcc accgggcacc cggaagaact ggctctcgat gacgttgccg atcatcttgg
    10141 tgttgtccga cccgagcagt tcggcgttga cgtagtcacc ggctgcgggg atgaaggtca
    10201 gcagcgttcc ggcgagcacg cccggcatgc tgatcggcag cgtcaccgtg cggaaggtcg
    10261 tgaacccgtt ggcgtacagg tctccccctg cctcgatgac ccgggagtcc gcgcgctcga
    10321 gcgaggcgta gatcggcagc accatgaagg gcaggaagtt gtacgtcaga cccgcgacga
    10381 ccgcgatcca ggtctcggtg atccggccac cggggagcag gtgcagggtg ttcagcgtcg
    10441 acgcgaccgg accctcgtcc gcgaggatct ggcgccaggc cacggtgcgc aggatgaacg
    10501 aggtgaagaa cggcgcgatg accgcgatga gcatgacgtt gcgccagcga ccggccttga
    10561 acgccatcgc gtaggccagc gggtaggcca ggcagaacgc gaagaacgtc gcgagggtgg
    10621 cgaacaggat cgagcggccg aagtgcgggg cgaagtcggt cagggccgtg acgtagttgc
    10681 cgaagttcag gtcgcggtag tagtacccgg ggtagccggg gaactgcgac tggaggctga
    10741 ccgagaacag ctggccgagg gggatgacga agaacacgag cagccagagc gcaccgggca
    10801 gcaggagcag gtagggaacc cagctccggc ccttgcggtt gggctgcggt gccgggggcg
    10861 gcgtggtgcc acccgcggaa ctcacgtggg caagggcgct catgtcaggc cagcctccgc
    10921 cgcagcccgg gcctcggcct cgtcgacggg gacgccgaac gcgtggtggg ggttccagga
    10981 cagccacacg tcgtcacccg gacgcaggtc gatcggctcg acgtcgaggt tctgctcgta
    11041 gcagctccac accgtgccgc tcggcagctg cacgaggtac tgggtggcga ccccggtgaa
    11101 cgacacgtcg agcacgatgc ccttgacgtc gtcgccggca ccctcggagg ggcggcgcat
    11161 gacgcgcacc ttctcggggc gcacaccgaa cgtgatgtcg ccgtcggtga ccgcgctgcg
    11221 cgcgatgggg atcttgacct tggtgccctg gatctcggcc acgaggtggg tgccgtcgcg
    11281 gtcgatgacc cggccgctgc cgaggttgga ctgacccacg aagttggcga cgaaggccgt
    11341 gcgcggcagg tcgtacatct gcgcgggcgg gcccatctgc tcgatcctcc cgtggttcat
    11401 cacggccacg gtgtcggcca tggtcatggc ctcctcctgg tcgtgcgtga cgtggatgaa
    11461 cgtcaggccc acctcggtct ggatgcgctt gagctcgacc tgcatctgac gccgcagctt
    11521 gaggtcgagg gcgccgagtg gctcgtcgag caggagcacc tcggggcggt tgacgatggc
    11581 ccgggccagg gccacccgct gctgctgacc acccgagagc tgggcgggct tgcgcgcggc
    11641 gaggtgatcc atctgcacga gcttgagggc atcctcggcg agcgcggcgg catccgcctg
    11701 tccccggcgc ttgggaccga acgcgacgtt gtcgcggatg gtcaggtgcg ggaagagggc
    11761 gtagctctgg aacaccgtgt tgaccggacg ctcgtaggag cggctgccgg tgaggtcggt
    11821 gtcgccgatg agcaggcgcc cgctcgtcgg ctgttcgagg ccggccacca tgcgcagggt
    11881 cgtcgtcttg ccgcagccgg acgggccgag cagggcgaag aacgagcccc gcgggacgac
    11941 caggctgagg tcgtcgacgg cggtgaactc ggcgaaggac ttcgtgacgt tctccagacg
    12001 caggtcgccc tgtgcgtcgc ggaaaccgct tgcgttcttg cgagccatca gttgcccatc
    12061 accttctgcc agatggcgct gtattcctgg tcctcctcgg gcgagagtgc ccggaaccct
    12121 tggatgttgt tgtccgcgat gaacgcctcg ctcgggaaga tgaacgggct ctcggcgagc
    12181 tcgggatcgg tcttcgcgag cacctcccgg gcgccgacga cggggcacac gtagttgacc
    12241 caggccgcga cctcggcagc gacctcgggg tcgtagtaga agttcatcag ggtctgggcg
    12301 ttgcggcggt gctgcgaggt gatcggcacc atcatgttgt cgctccagat cgtgccgccc
    12361 gactccggga tgacgaactc ccagttctcg ttctcggtct cggcgcgcag gatgaacagg
    12421 tcaccgctcc agacgatgcc ggcgacggcg ttgcccgact tgaggtcctc gaggtaggag
    12481 ttgcccttga tccggcggat gtagccctcg tccatgcgct tctcgatctc ggccacggcc
    12541 gcctcgaact ccgccttgcc gaactcggag gtgatgtcga cgccctggct ctgcatgatg
    12601 atgcccacgg tgtcgcggaa ctcgctgagc acgacgatct tgcccttgag ctcgtcggtc
    12661 cacaggtcgt cgagcgtctt gatctccttg gagaccttgg acttgtcgta gccgatgccg
    12721 gcgaagccgg actgccaggt cagcgagaag ttgcggcccg ggtcgaagct gacgttctgc
    12781 agcgccggca gcaggttcgg tgcgttgggc atggtgatca gctcgagcgg ctggcacagc
    12841 tggtcgcgga tgatccggtt tgccatccag tcggtgaagg tgaagatgtc gcgcccgatg
    12901 tcctggcccg cgcgaagctg gggcgcgatc ttgttgaagt acgcgtcgtt gtcctcgatg
    12961 tcctcggtgt actcggcacg gatgccggtc tcggcgatga accgctcgag ggtcgggtac
    13021 ttcttggtct cctcgtcgta gtcgaggtag gccgtccagt tggcccagcg caccaccttg
    13081 tcggtgtcgg agaggtcctc gggcagcacg agcgaggcga ccccgcccga gggtgggggc
    13141 ggtggtgcgc acgcgctgag cgccgccgcc gcgccaccga gggcggccgt gccgaacagg
    13201 gagcgccgcg tcagcgccga ggaccgtgcg gcgctgatca gcccgcgggt catgggatcg
    13261 gccgggggcc gccgtcgaag tgccatgtgg tgtgttcctc agctctcgat gttcgccatg
    13321 acgtgcttga tgcgcgtgta gtcctcgaac ccgtacatcg acaggtcctt gccgtagccc
    13381 gacttcttgt acccgccgtg gggcatctcg gcgacgagcg ggatgtgggt gttgatccac
    13441 acgcagccga agtccagggc cttgctcatc cgcatggccc ggccgaagtc cttggtccac
    13501 accgaggacg cgaggccgta gtcgacgccg ttggcccaac gaatcgcctc ggcctcgtcg
    13561 gtgaacttct gcacggtgat gaccgggccg aagatctcgt tctggatggc ctcgtcgtcc
    13621 tggcgcagcc cggagagcac ggtgggctcg aggtagtagc cctcaccgag gtcggtgcgg
    13681 cggttgccgc ccgccgagac cgtcgcgtgg gagggcagtc ggtcgatgaa gccggagacg
    13741 tgagccagct gacgggagtt gttgaccggg ccgaagaggg cgtcctcgtc gtcggggagg
    13801 ccgaccgggg ccgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtgcgg
    13921 tgctcgacga gcatccggcc ggtctcgcgg tcaccggtga tgacgttcag ggtgcccgcc
    13981 gggaggaact gcgaggccag ctcggccagg cgcagggtgc tctcgggggt ggtgtcgctc
    14041 ggcttgagga ccacgctgtt gcccgccgcc agggccgggc cgatcttcca gaccgccatg
    14101 agcagcgggt agttccacgg ggtgacctgc ccgacgacgc cgatcggttc gcggcggatc
    14161 cacgaggtgt gaccggccat gtactcggcc gaggcccggc cctcgaggac gcgggccgcg
    14221 ccggcgaaga agcgcagctg gtcgaccatc ggcgggatct cctcactcgc ggtgagggcc
    14281 ttgatcttgc cggtgttctc ggactccagc gcgatgagct cgtcagcgtg cgcctccacg
    14341 gcatccgcga acttcagcaa cgcctgctgg cggtcgctgg gggtcgtggc gccccactca
    14401 cccgcgaagg cctgctcggc ggcggcgtag gccgcgtcga cgtcctcctg accgctgacc
    14461 ggggcggtcg cgacgaccga tcccgtcgca gggttgatga tctcggtggt ggaatctgtc
    14521 cgcccgtcga cgtactcgcc gccgatgaag ttgcgcaggt gtcgctgacc gctcatgggt
    14581 gatggctccc aaaggggtgg ggggtgggtt cggtgctacc ggactctaag cgcacgacaa
    14641 ccgaaatcgc tagggattgc gcgacagatg tgatgaaaag ttacacaccg gacacacaat
    14701 gcatacggaa tccgttccag gggtgcttgc aaaccgtcgg gatccgcgac acgatggcgc
    14761 gcgtgacctc cacctctcga ccccccaggg tgacgcgtgc atcgtccgag acccgtctgg
    14821 acgacatcgc caaacacatc atcgaactgc tccaggaaga cggccggcag tcctacgccg
    14881 cgatcgccaa ggcggtcggc ctctccgagg ccgctgtgcg ccagcgggtg cagcgcctgc
    14941 tcgacgccga cgtcatgcag atcgtcgccg tgaccgaccc cctccaggtg ggcttcagcc
    15001 gccaggcgat gatcggcatc cgggtatccg gggacctgct cgccgtcggc gacgccctca
    15061 cggcgatgcc cgaagtcgac tacgtcgtga ccacggccgg cagcttcgac atcctcgccg
    15121 aggtcgtctg cgaggacgac gaccacctcc tggacctgct caaccagcag atccgcaccc
    15181 tccccggcgt cacgagcacc gagaccttcg tctacctgaa actcaacaag cagcactaca
    15241 actggggtac ccgatgagca cctacccgtc cgacgtctgg gagcccagct ccgatcgcca
    15301 caccgccacc ggcgccctgt actccgacgc cgcccgcgac cacctgtgga tgcacttcac
    15361 ccggcactcc accttcgagg ggcacggcct cggccactcc gtgccgatca tcaccaaggg
    15421 tcagggctgg acgatctggg acgaccgggg caacgagtac atcgacgccc tcgccggtct
    15481 gttcgtcacc aacgtcggcc acggacgcac cgagatcgcc gaggcgatgg ccaagcaggc
    15541 cagcgagctc gcgttcttcc cgttgtggtc ctacgcccac ccgcgggcca tcgagctcgc
    15601 cgagcggctg gcgcaccacg ccccgggtga cctcaaccgg gtgttcttca ccaccggcgg
    15661 cggcgaggcc gtcgagacgg cgtggaagct ggccaagcag tacttcaagc tgaccggcaa
    15721 gccgaccaag cacaaggtca tctcgcgctc gatcgcctac cacggcaccc cccagggggc
    15781 gctgtcgatc accggcatcc cggcggcgaa gtcgatgttc gaaccgctcg tccccggcgg
    15841 ccacaaggtg cccaacacca acatctaccg cgcccccgag cacctgcgcg acgacgagaa
    15901 ggcgttcggt cgctgggccg ccgaccgcat cgccgaggcc atcgagttcg agggcccgga
    15961 gaccgtcgcg gccgtcttcc tcgagcccgt gcagaactcc ggcggctgct tcccgccgcc
//
LOCUS       DAOSOC010000042        15270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3907_length_15270_cov_9.480315, whole genome shotgun sequence.
ACCESSION   DAOSOC010000042 DAOSOC010000000
VERSION     DAOSOC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 15270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15270
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3907_length_15270_cov_9.480315"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PL014_05150"
     CDS             complement(<1..218)
                     /locus_tag="PL014_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56510.1"
                     /translation="MSIFKRSKKSDTAGRADEVDGVETLEGQGSVDTDADGTQGADGD
                     AAGDDAVTRTASVVLDRSEGPFDASEVDG"
     gene            complement(256..720)
                     /gene="dut"
                     /locus_tag="PL014_05155"
     CDS             complement(256..720)
                     /gene="dut"
                     /locus_tag="PL014_05155"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT56511.1"
                     /translation="MRSLSTVAVRRLHPHARLPLYATQGDAGADLTSVAQVTLAPGER
                     ALVPTGIALALPPGTVGLVHPRSGLAARHGITVVNAPGTIDSGYRGEVHVNLVNLDPR
                     ESFTVQVGDRIAQLVVQHYVHATFEVADSLPESDRGDTGHGSTGGFGPHQPD"
     gene            complement(708..1007)
                     /locus_tag="PL014_05160"
     CDS             complement(708..1007)
                     /locus_tag="PL014_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HOT56512.1"
                     /translation="MATDYDAPRKTDDELSEDSIEELKSRRVDKRASSVDVDETEQAE
                     GFELPGADLSGEELSVRVLPRQADEFTCSRCFLVHHRSQLATDKGGVLVCRECAA"
     gene            complement(1155..2030)
                     /locus_tag="PL014_05165"
     CDS             complement(1155..2030)
                     /locus_tag="PL014_05165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT56513.1"
                     /translation="MTTHPAATASDLPVGLAAVLEEIACTVARAAGRLVVDERPVDLG
                     VSSKSSATDPVTVMDQRSQELIIELLAAARPDDAVLGEEEGGSTGTSGLTWVIDPIDG
                     TVNYLYGIPAYAVSVAVVVGDPAVAGAWQPVAGAVLNPESGELFHAHLGGGARLTTDA
                     GTRTLRTTAATDVAHALVGTGFGYAADLRRRQAELLVDLLPRVRDIRRHGGAALDLCG
                     LAAGRLDAYYESALNPWDRAAGELVAREAGAVLGGPVGDVLTRDLVWGAAPGLAAQFG
                     PLVRELSARHVGSVD"
     gene            complement(2030..3139)
                     /locus_tag="PL014_05170"
     CDS             complement(2030..3139)
                     /locus_tag="PL014_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOT56514.1"
                     /translation="MPDSASPLAPYDAVLLVSFGGPEAPEEVVPFLRRVTAGRDIPDA
                     RLEEVGEHYFARGGRSPINDLNRDLLARLRAELDRREISTPLLWGNRNSEPFLAGAVH
                     EALDQGASRVLALVTSAYSCYSSCRQYREDLAAAVDEVGPAADGLVIDKVRPYSGHPG
                     VGRAWRRALVAALSELPEPASARILYVTHSIPTAMDDTSGPGDGEGNLYVDQHVRLGR
                     RLTDEVNAELGLDVEGELVFCSRSGRPGQPWLEPDVNDRIEELAAEGGVSAVVVAPIG
                     FVSDHMEVVHDLDTEAAETAQKVGMPFVRVATPGTDDLFVEGLVDLLLERAAEARGEV
                     VLPETWVPGDVRPSVCAPGCCPNLRAVKPALCGSD"
     gene            3249..>3355
                     /gene="sepH"
                     /locus_tag="PL014_05175"
     CDS             3249..>3355
                     /gene="sepH"
                     /locus_tag="PL014_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HOT56515.1"
                     /translation="MQDLRLIGVHEDGQHLLLANADGGRFRLPLDEALRA"
     assembly_gap    3356..3455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3456..4307
                     /gene="sepH"
                     /locus_tag="PL014_05180"
     CDS             <3456..4307
                     /gene="sepH"
                     /locus_tag="PL014_05180"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HOT56516.1"
                     /translation="ADRVADRLKDRGVERDAVDWDSARDEEGTWQLSMTFSAGGRQRT
                     ATWRYEPLGGSVTATNDEARWLSEDATPGAIPTPHRAVLEGDLDVYDIDADGGLERAP
                     RERGPRERGPHDPIDLMAAMREQSARGRKRRRSSPTHTPGGDGLHGDALPLEDLHLDP
                     TFVPPPPPPVAVPAEAVSTETESHADARGDTPEAEDDVTSDAEEFIVHDLPVDDAGEG
                     DPIADDAASDTDLASSEAEHTADDPASERSGPKRPPMRPTPSRKGRPSVPSWDDIVFG
                     TKGSGQG"
     gene            complement(4325..5014)
                     /locus_tag="PL014_05185"
     CDS             complement(4325..5014)
                     /locus_tag="PL014_05185"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOT56517.1"
                     /translation="MAELVFFSGTMDCGKSTLALQMDHNNSARGRQGLIFTKLDRMGE
                     SVLSSRLGLSTPAIEVSDHLDFWDAVVERLTQGVRVDYLICDEAQFYTPVQVEQLARL
                     VDELGIDVHAFGITADFRTELFPGSRRLIELADRVQLLQVEALCWCGRKATHNARIVD
                     GVMVVEGEQVVVGDTASTSVGLVEYEVLCRRHYMRRMNSHAARAAALSPDTLPFDLDV
                     CAVPPQPPSRA"
     gene            complement(5026..>5736)
                     /locus_tag="PL014_05190"
     CDS             complement(5026..>5736)
                     /locus_tag="PL014_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT56518.1"
                     /translation="GVEVVRIGPAYFDGSGLTTAALRGGRFVAATSLEARVDAALAAV
                     RAAPRTLVHLYWGELDKVGHVHGCDSWQWGEELEAVDRELARLAARLPADCSLTVTAD
                     HGMVDSPHELRLDVAHEPVLAHGVRHVSAEARAPQLYVEPGAADDVRAAWAETLGERA
                     LVRSRAEAVAAGWFGAVLPENLPRIGDVVVAMRGRFAVEDSRRARPELLALIGLHGSL
                     SDDEVSVPVVHVPPAVVA"
     assembly_gap    5737..5836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5972..6571)
                     /locus_tag="PL014_05195"
     CDS             complement(5972..6571)
                     /locus_tag="PL014_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HOT56519.1"
                     /translation="MDPMSPAPDAPVLPADLTAAIVRAGYYPALVADVVAAALVHEPI
                     HSHLVHLETTFDRDVVRRHITVLVLTPTRLLIAHADDHADEPPAPQDVATATTESIPL
                     SAVRGVMLTHVVESPSTYVPGSLGREITLTLGWGTVARLDLVPASCADPDCDADHGYE
                     GTITGDDIALRISAAADGEDALAEALSFARDLSAALGQA"
     gene            complement(6620..9286)
                     /locus_tag="PL014_05200"
     CDS             complement(6620..9286)
                     /locus_tag="PL014_05200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT56520.1"
                     /translation="MDDDPLPPGYPSEWEADVVLRDGTVAHVRPIRPSDTDGIHRFHS
                     GQSDESIYLRFFAPLRRLSDADVHRFTHVDYVDRVALVVTLREDIVGIGRYDRIDARS
                     AEVAFNISDHYQGKGIGSVLLEHLAAIARDVGITRFTAEVLPQNRKMLSVFSDAGYDV
                     TRHIEDGVVEVGFDIEPTDSSKAVAMSREHRAEALSVAAILHPRTIAVIGASRRRNSI
                     GSQLLERIVAAGFTGEIHPVNPNVRTLRRRTAYPSVAAVPGRVDLAVIAVPAHAVLEV
                     VDECAEAGVKALLVVSGGFAEEGRDGARLQAELLRRARGAGMRVVGPNSFGLINNDPS
                     LRLNASLAMTLPPVGRVGLFAQSGALGIAVLASAARRNLGISTFGSAGNRVDVSGNDF
                     MQYWIDDDNTEVVGLYLESMGNPRKFSRIARNLALIKPVIVVKSGVSRFGVPPGHRVR
                     RTKARPAVFKAMLKQAGVLRVENVHQLFDIAQLLATQPLPRGDRVAIVGNSTALGTLT
                     ADACTSWGLKVAHGPVSLPSEATAAQFRTALAAAFADPKVDSVLTCFIPPLVTNDEDV
                     AAAVRDMAHGADKPCAATFLGMRGVDDGHSSVTGTGGSTQAIPVYAMPEDAVRALAAA
                     TRYGAWRSKDHGVPVAPAGINRRIAEDIVETVLAMEPKGRRLTSDESTALLQAYGIDV
                     WTKQEVSTVDEAVAAAEQVGYPVVLKSTAPMVRHQGGTSGVRVDLRTEAALRAAWESL
                     TERLAPLDADRLVVQRMATPGVPCVVTSDEDPLFGPVIGFSVAGLPTELLGDIGYRIP
                     PLTDVDVSELISSVKASPVLHGHRGATPVNRAALADLIARVSVLADDLAEVASLVLNP
                     VNAHPGGVEVLGAEIVLAPAPRRTDPGRRSLT"
     gene            9355..9984
                     /locus_tag="PL014_05205"
     CDS             9355..9984
                     /locus_tag="PL014_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT56521.1"
                     /translation="MELRDAVLRRRMVRRFDPSVPVEMEVVRDLVALAVRAPSAGFSQ
                     GWDFVALLNPQDRAAFWAAADDGGPADAWLRGVSAAPALVLCLSDAGAYLDRYAQADK
                     GWVDRSPDRWPIPYWDTDTAMAAMIILLGAQDAGLGALFFGIPAQRHDAVREAHGIPE
                     DRRLVGVIALGTPAARAAHTGGSPRTRPRRPLEDVLHAGRFGGWSGASA"
     gene            10073..12526
                     /locus_tag="PL014_05210"
     CDS             10073..12526
                     /locus_tag="PL014_05210"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOT56522.1"
                     /translation="MAKRTTTPPPGETPQDGVERVVDIDVEDEMRSAFLEYSYSVIYS
                     RALPDARDGLKPVQRRILFGMSELGLRPDRPHVKSSRVVGEVMGKYHPHGDQAIYDAL
                     VRMAQPFTMRLPLVDGHGNFGSLDDGPAASRYTEARMAPAALLMVSGLDEDTVDYVPN
                     YDDSLLQPGVMPAAYPNLLVNGASGIAVGMATNMAPHNLVEVIGAARHLIAHPDCSLD
                     DLMKFVPGPDLPGGGRIVGLDGIRDAYLTGRGSFRTRAAARVEKLTPRRMGIVVTELP
                     YLVGPEKVIERVKTLVQAKKLQGISDVKDLTDRKNGLRLVFEVKNGFNPDAVLEQLYK
                     LTPMEDSFGINNVALVDGQPRTLGLKELLRTYVDFRIEVVRRRTEFRLRKRQDRLHLV
                     EGLLIAIVDIDEVIQLIRTSDDAATAKARLMSVFDLSEAQAEYILELQLRRLTKFSLI
                     ELESERDELRRQIEELEAILADEKLLHRTVSGELADVAKAHGTPRRTVLLESAGVPAS
                     VGAPLEVADDPCWVLLSSTGLLARTSSADPVPADGGRAKHDAIIGAVRTTARGEFGLV
                     TSAGRMLRMSALELPTLPPLGGAPSLSGGAPLAVFADLPAGEEPLTIVGLDAEGPGIA
                     LGTAHGVVKRVTPDHPARANWEVIALKDGDRVVGAAALRDGEEDLVFVTSDAQLLRFS
                     ASSVRPQGRAAGGMAGIRLNPGASVVFFGAVDPTADAVVVTSSGASDALPGTQPGAIK
                     VTPYAEYPPKGRGTGGVRAHRFLRGEDALILAWVGAQPPRASGAAGVALELPEASGRR
                     DGSGTAYPAVIGGIGSPVR"
     gene            12736..14103
                     /locus_tag="PL014_05215"
     CDS             12736..14103
                     /locus_tag="PL014_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT56523.1"
                     /translation="MALYTKRRIAALSGVLAVALTATACGSSSDDGGDTAESGSTSSI
                     DCAPYTAFGDLKGKTVTVYTSITAPEDAPHIASYQPFVDCTGVEIKYEGSKEFEAQLP
                     VRVKGGNAPDIAYLPQPGLLNTLVQAGAVKAAPPEVIANVDKNMPDWKNYGTVDGTFY
                     AAPLGANVKSFVWYSPSAFADKGYEVPTTWADLLALSEKMAAEGGDVKPWCAGIESGD
                     ATGWPATDWVEDVMLRTAGAETYDKWVNHEIPFNDPAVATALAEVGKILKNDKFVNGG
                     FGDVKTIASTSFQDAGQPILDGTCYMHRQASFYAANWPEGTEVGPEGEVWAFYLPSVD
                     SSTKPVLGGGEFTAAFADRPEVKAFQTYLSSVEWADERAKTCGSGGCVTANKNADPSL
                     LKNPVDKLSAEALTDSAATFRFDGSDLMPGAVGAGTFWKGMTDWIIGKDDKATLDFIE
                     QSWPK"
     gene            14309..15238
                     /locus_tag="PL014_05220"
     CDS             14309..15238
                     /locus_tag="PL014_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT56524.1"
                     /translation="MFFAIFFFALVFGLVLFLASLFKGKNGERVQGALFVGPAVVLLA
                     IGLMYPAILTINQSLRGRSGEGGLTLSNYSAMFTQPELLLVLRNTALWVILVPILATS
                     IGLIYAILIDRARGEAFAKALIFLPMAISMVGAGIIWKFVYQYKQTSRPQIGLLNAIL
                     KLLGFETQQFLINAPLNTFMLIIVMVWIQAGFAMTVLSAAIKAIPDEIIEAAKLDGVQ
                     AFKMFRYITLPSIRAALVVVVTTIGIGTLKVFDIVRTMTGGQFDTSVVANEFYSQSFR
                     FNAPGLGAALAVLLFILVIPIVTYNIIQMRKEA"
BASE COUNT         2203 a         5359 c         5368 g         2140 t
ORIGIN      
        1 ccgtcgacct cgctcgcgtc gaacgggccc tcgctgcggt cgagcaccac ggatgccgtg
       61 cgcgtcaccg cgtcgtcgcc cgccgcgtca ccgtcggcgc cctgcgtgcc gtccgcgtcg
      121 gtgtcgacgg agccctggcc ctccagcgtc tcgacgccgt cgacctcgtc ggcacgacct
      181 gcggtgtcgg acttcttgga acgcttgaag atgctcacgg gggtcgtcct cgaatctggt
      241 ctggagtgct gcgggtcagt cgggctggtg cgggccgaag ccgcccgtgg acccgtgccc
      301 agtgtccccc cggtcactct ccggaagcga atccgcgacc tcgaaggtcg cgtgcacgta
      361 gtgctgcacg acgagctggg cgatgcggtc accgacctgc accgtgaacg actcgcgcgg
      421 gtcgaggttc acgaggttga cgtgcacctc gccgcggtat cccgagtcga tggtgcctgg
      481 agcattgacc acggtgatgc cgtgccgggc cgcgaggccc gagcgcgggt gcaccagccc
      541 gaccgtgccc ggcgggagcg ccagcgcgat gccggtgggc accagcgccc tctcccccgg
      601 ggccaacgtc acctgcgcga cgctcgtgag gtcggcaccg gcatccccct gagtggcgta
      661 gagcggcagg cgggcgtgcg ggtgcagccg gcgaacggcg acggtgctca ggctgcgcac
      721 tcccggcaca cgagcacgcc acccttgtcg gtcgcgagct ggctgcggtg gtgcacgagg
      781 aagcaccgcg agcaggtgaa ctcgtcagcc tggcgcggca ggacccgcac cgagagctct
      841 tcgccggaca ggtcggcacc gggcagttcg aagccctcgg cctgctcggt ctcgtcgacg
      901 tcgacactcg aggcgcgctt gtcgacgcgc cggctcttca actcctcgat ggagtcctct
      961 gacagctcgt cgtcagtctt gcgcggtgcg tcgtagtcgg ttgccattgc ctgtcgtcca
     1021 cccttcgtcg tgtcccggga ccctcagaac gcatcaggtt ccgattttgt gcccgggctg
     1081 cgccgtgcgc ggcgattgtg caccatcgac ggcacaaaag ccatgtcgtc ccagcgggca
     1141 ggaagaaacc ggcctcagtc caccgagccg acgtgccggg ccgacagctc acgcacgagc
     1201 ggaccgaact gcgccgccag ccccggagcg gccccccaca cgaggtcgcg cgtgaggacg
     1261 tcaccgaccg ggccgccgag cactgccccg gcctcacggg cgacgagctc cccggcggcg
     1321 cggtcccacg ggttgagggc gctctcgtag taggcgtcga ggcggcccgc ggcgaggcca
     1381 cacaggtcga gggcggcccc gccgtggcgg cggatgtctc gcacccgcgg caggaggtcg
     1441 acgagcagtt cggcctgtcg ccggcgcagg tcggcggcgt agccgaaccc cgtgccgacg
     1501 agggcgtgcg cgacgtcggt ggcggccgtg gtgcgcaggg tgcgcgtccc ggcatccgtc
     1561 gtcagtcgtg ctcccccgcc gaggtgcgca tggaagagct cgcccgactc agggttcagc
     1621 accgcgcccg ccaccggctg ccacgcaccg gccacggccg ggtcccccac gacgacggcg
     1681 acggacacgg catacgccgg gatgccgtag aggtagttga cggtgccgtc gatcgggtcg
     1741 atcacccagg tcagcccgct ggtgccggtc gagccgccct cctcctcacc gaggacggcg
     1801 tcgtcggggc gggccgcggc gagcagctcg atgatgagct cctggctgcg ctggtccatg
     1861 acggtcacgg ggtcggtcgc gctcgacttc gacgacacgc ccaggtcgac cggccgctcg
     1921 tcgacgacga gtcgcccggc ggcgcgcgcg accgtgcagg cgatctcctc gaggacggcg
     1981 gcgaggccga cgggcaggtc ggatgccgtg gcggcgggat gcgtggtcat cagtcgctgc
     2041 cgcacagggc cggcttcacg gcgcgcaggt tggggcagca gcccggggcg cacaccgagg
     2101 gacgcacgtc accgggcacc cacgtctcgg gcaggacgac ctcgccgcgg gcctcggccg
     2161 cgcgctcgag cagcaggtcg accagaccct cgacgaacag gtcgtcggtg cccggcgtcg
     2221 cgacccgcac gaacggcatg ccgaccttct gcgcggtctc ggccgcctcg gtgtcgaggt
     2281 cgtggacgac ctccatgtgg tcggagacga acccgatcgg tgcgacgacg acggcgctga
     2341 ccccaccctc ggcggcgagc tcctcgatgc ggtcgttgac gtcgggctcg agccacggct
     2401 ggccggggcg acccgaccgg gagcagaaga cgagctcacc ctcgacgtcc aggccgagct
     2461 cggcgttgac ctcgtcggtg aggcggcgcc ccagccgcac gtgctggtcg acgtagaggt
     2521 tgccctcgcc gtcgcccggg ccggacgtgt cgtccatggc cgtcgggatg gagtgggtga
     2581 cgtagaggat gcgcgcgctc gccggctcgg gcagctcaga cagggccgcg acgagggccc
     2641 ggcgccacgc cctcccgaca cccgggtgcc cggagtacgg gcgcaccttg tcgatgacga
     2701 ggccgtcggc ggccgggccg acctcgtcga ccgctgccgc gaggtcctcg cggtactgac
     2761 ggcacgagga gtagcacgag taggcgctgg tcaccagggc gaggaccctg gacgccccct
     2821 ggtccagggc ttcgtggacc gctccggcga ggaacggctc ggagttgcgg ttgccccaga
     2881 gcagcggggt ggagatctcg cggcgatcca gttccgcccg caggcgcgcg agcaggtcgc
     2941 ggttgaggtc gttgatcggg ctgcgcccgc cgcgggcgaa gtagtgctca ccgacctcct
     3001 cgaggcgggc gtcggggatg tcacggccgg ccgtgacgcg gcgcaggaag ggcacgacct
     3061 cctcgggggc ctcggggccg ccgaaggaga cgaggaggac ggcgtcgtac ggggcgagtg
     3121 gggacgcgga gtcaggcatg cgacaagcgt aagcgggcaa tccggcgagc ctcggcgtgg
     3181 gctgcggcat ctggccgtcg ggcagtggca cagtcgtcct gaccacgagc accaccggga
     3241 gcccccacat gcaggacctt cgactcatcg gcgtccacga ggacggccaa cacctgctgc
     3301 tggcgaacgc cgacggcggg cgcttccggc tgcccctcga cgaggccctg cgcgcnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccga tcgggttgcc gatcgcctca
     3481 aggaccgcgg ggtcgagcgg gatgccgtgg actgggactc cgcgcgcgac gaggagggca
     3541 cctggcagct gagcatgacc ttcagcgccg gaggacgcca acgcaccgcg acctggcgct
     3601 acgaaccgct cggcgggtcg gtgacggcca ccaacgacga agcacggtgg ctgagcgagg
     3661 acgccacacc cggggcgatc cccacccccc accgggccgt cctcgagggt gacctggacg
     3721 tctacgacat cgacgccgac ggtggcctcg agcgtgctcc tcgggagcgc gggccccgtg
     3781 aacgcggccc gcacgacccg atcgacctca tggcggccat gcgcgagcag agtgcccggg
     3841 gtcgaaagcg ccgacggtcc tcacccacgc acacccccgg aggtgacggg ctgcacggcg
     3901 atgccctgcc gctggaggac ctccacctcg acccgacgtt cgtgccgccc ccgccgcctc
     3961 ccgtcgccgt gcccgcagag gccgtgtcca ccgagaccga gtcgcatgcg gatgcccgcg
     4021 gggacacacc ggaggccgag gacgacgtga cttcggacgc cgaggagttc atcgtccacg
     4081 acctgccggt cgatgacgcc ggcgagggcg atcccatcgc tgacgacgcg gcatccgaca
     4141 ctgacctggc gtcttccgag gccgagcaca ccgccgacga cccggcatcc gagcgatccg
     4201 ggcccaagcg tccgccgatg cgtccgacgc cgtcgcgcaa ggggcgcccg agcgtgccga
     4261 gctgggacga catcgtcttc ggcaccaagg gcagcggcca gggctgaccc ggccgaccgc
     4321 cgtgtcaggc gcgagaaggc ggctggggcg gcacggcgca cacgtcgagg tcgaacggca
     4381 gcgtgtccgg cgagagggca gcggcccgcg ccgcgtggct gttcatccgc cgcatgtagt
     4441 ggcggcggca gagcacctcg tactcgacga gcccgaccga agtgctcgcg gtgtcgccga
     4501 cgacgacctg ctccccctcg acgaccatca ccccgtcgac gatgcgggcg ttgtgggtgg
     4561 ccttgcgccc gcaccagcac agcgcctcga cctgcagcag ctgcacccgg tcggcgagtt
     4621 cgatgagccg gcgcgacccg gggaagagct cggttcggaa gtcggcggtg atgccgaagg
     4681 cgtgcacgtc gatgccgagc tcgtcgacga gccgggccag ctgctcgacc tgcacggggg
     4741 tgtagaactg cgcctcgtca cagatgaggt agtccacccg gacaccctgg gtgagccgtt
     4801 cgacgacggc gtcccagaag tcgaggtggt cggagacctc gatcgccggg gtcgacaggc
     4861 ccagccgcga ggagagcacc gactccccca tgcggtcgag cttggtgaag atcagcccct
     4921 gccgtccgcg ggcggagttg ttgtggtcca tctgcagggc aagggtcgac ttgccgcagt
     4981 ccatcgttcc ggagaagaac accagttcag ccacgaggcg acaccctagg cgacgacggc
     5041 cggtggcacg tgcacgacgg gaaccgacac ctcgtcgtcg ctgagcgacc cgtgcaggcc
     5101 gatgagcgcg agcagttcag ggcgggcgcg gcgggagtcc tcgacggcga accggccccg
     5161 catcgcgacg acgacgtcgc cgatccgcgg caggttctcg ggcagcaccg cgccgaacca
     5221 cccggcggcg acggcctccg cccgactgcg gacgagagcc cgctcgccga gcgtctcggc
     5281 ccaggcagcc cggacgtcgt cagcggcgcc gggttcgacg tagagctgtg gggcccgggc
     5341 ctcggcactg acgtggcgca ccccgtgggc cagcaccggc tcgtgggcga cgtcgaggcg
     5401 cagctcgtgc ggggagtcga ccatgccgtg gtcggccgtc acggtgagcg agcagtcagc
     5461 cggcagccgg gcggcgagcc tggccagttc ccggtcgacg gcctcgagct cctcgcccca
     5521 ctgccaggag tcgcagccgt gcacgtggcc gaccttgtcg agctcacccc agtacaggtg
     5581 caccagggtg cgcggcgcag cgcggacggc ggcgagcgcg gcgtcgaccc gggcctcgag
     5641 ggaggtcgcg gcgacgaaac ggcctccacg cagggcggcc gtggtcagcc cggatccgtc
     5701 gaagtacgcc gggccgatgc gcacgacctc gacgccnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnggtc acctcccgct cgcggcgtgc cccacgcgac acccaggctc
     5881 ctggcgacgc tgggcagcac gtcggccagc gagggcgcgg acccgacggg tgcggccgcc
     5941 gacgggtccc cggtggacgg catccggggg ctcaggcctg gccgagggcc gcggacaggt
     6001 ccctcgcgaa cgacagcgcc tccgccaggg cgtcctcacc atcggctgcg gccgagatgc
     6061 gcagggcgat gtcgtcaccg gtgatggtgc cctcgtagcc gtggtcggcg tcgcagtcgg
     6121 ggtcggcgca ggaggccggc acgaggtcga gccgggccac cgtgccccac ccgagggtga
     6181 gggtgatctc gcggccgagg ctgcccggga cgtacgtcga cgggctctcg acgacgtggg
     6241 tcagcatgac gccgcgcacc gcggacagcg ggatcgactc ggtggtcgcg gtggcgacgt
     6301 cctgcggggc gggtggctcg tcggcgtggt cgtcggcgtg ggcgatgagc agccgggtgg
     6361 gggtcagcac gagcaccgtg atgtggcggc gcacgacgtc gcggtcaaag gtggtctcga
     6421 ggtggacgag gtggctgtgg atcggctcgt gcaccagggc tgcggcgacg acgtcggcga
     6481 ccagggctgg gtagtagccg gcccgcacga tcgcggcggt gaggtccgcc gggaggacgg
     6541 gggcgtcggg ggcagggctc atggggtcca ttgtgcccgc tgccggtgac agccacgtca
     6601 gcgaacggcg tggcctgcgc tacgtcagcg accggcgccc cgggtcggtg cggcggggtg
     6661 cgggggcgag cacgatctcg gcgccgagca cctcgactcc cccggggtgg gcgttgacgg
     6721 ggttgagcac gagcgaggcc acctcggcga ggtcgtcggc gagcaccgag acccgcgcga
     6781 tgaggtcggc cagggcagcc cggttcaccg gggtcgcccc gcggtggccg tggagcaccg
     6841 gggacgcctt caccgacgag atgagctcgg agacgtcgac gtcggtgagt ggcgggatgc
     6901 ggtagccgat gtcaccgagc agctcggtgg gcagcccggc gacgctgaag ccgatgaccg
     6961 gcccgaagag cgggtcctcg tcggaggtga cgacgcaggg cacccccggg gtcgccatgc
     7021 gctgcacgac caggcgatcc gcgtccaggg gtgcgagccg ttcggtgagc gactcccacg
     7081 cggcccgaag ggcggcttcg gtgcgcaggt cgacgcgcac cccgctggtg ccgccctggt
     7141 ggcggaccat cggcgcggtc gacttcagca ccaccgggta gccgacctgc tcggccgccg
     7201 cgaccgcctc gtcgacggtg ctgacctcct gcttggtcca gacgtcgatg ccgtaggcct
     7261 gcaggagggc ggttgactcg tccgaggtga ggcgccgccc cttgggctcc atcgcgagca
     7321 ccgtctcgac gatgtcctcc gcgatccgcc ggttgatgcc ggccggcgcc acgggcaccc
     7381 cgtggtcctt ggagcgccag gcaccgtacc gggtggccgc ggccagggcg cgcacggcat
     7441 cctcgggcat cgcgtagacg gggatcgcct gggtcgagcc acccgtgccg gtgacggagg
     7501 agtgcccgtc gtccaccccg cgcatgccga ggaaggttgc ggcgcagggc ttgtcggcac
     7561 cgtgcgccat gtcgcgcacg gccgcggcga cgtcctcgtc gttggtcacc aggggcggga
     7621 tgaaacaggt cagcacggag tcgaccttgg ggtcggcgaa cgcggccgcg agcgccgtgc
     7681 ggaactgggc cgcggtggcc tcgctcggca ggctcaccgg gccgtgggcg accttcaggc
     7741 cccagctggt gcaggcatcg gcggtcagcg tgccgagcgc ggtcgagttg ccgacgatgg
     7801 cgacgcggtc gccgcgggga agcggttgcg tggcgagcag ctgggcgatg tcgaagagct
     7861 ggtggacgtt ctcgacgcgc agcaccccgg cctgcttgag catggccttg aacaccgccg
     7921 ggcgggcctt ggtgcggcgg acccggtgcc ccgggggcac cccgaagcgc gagacgccgg
     7981 acttcacgac gatgaccggc ttgatgaggg cgaggttgcg ggcgatccgc gagaacttgc
     8041 gcgggttgcc catcgactcg aggtacaggc cgacgacctc ggtgttgtcg tcgtcgatcc
     8101 agtactgcat gaagtcgttg cccgagacgt cgacgcggtt gccggccgag ccgaacgtcg
     8161 agatgccgag gttgcggcgc gcggccgagg cgagcacggc gatccccagg gccccggact
     8221 gcgcgaacag accgacccgc cccaccggtg gcagcgtcat cgcgagcgag gcgttgagcc
     8281 gcagcgacgg gtcgttgttg atcagcccga aggagttcgg cccgacgacc cgcatccccg
     8341 ccccgcgggc ccggcgcagc agttctgcct ggaggcgggc gccgtcacgg ccctcctccg
     8401 cgaacccgcc cgagacgacg agcagcgcct tgacccccgc ctcggcgcac tcgtcgacga
     8461 cctcgagcac cgcgtgggcc gggacggcga tgaccgcgag gtcgacccgt ccgggcacgg
     8521 ccgccaccga ggggtaggcg gtgcgccggc gcagggtgcg cacgttgggg ttgaccgggt
     8581 ggatctcgcc ggtgaacccg gccgcgacga tgcgctcgag cagctggctg ccgatggagt
     8641 tgcgccgccg gctggcgccg atgacggcga tcgtgcgggg gtgcaggatc gcggcgacgc
     8701 tcagggcctc ggcccggtgt tcgcgtgaca tggcgacggc cttcgaggag tcggtcggtt
     8761 cgatgtcgaa cccgacctcg acgaccccgt cctcgatgtg ccgcgtcacg tcgtacccgg
     8821 cgtcggagaa caccgagagc atcttgcggt tctgcggcag cacctccgcg gtgaagcggg
     8881 tgatgccgac gtcgcgcgcg atcgcggcga ggtgctcgag gagcaccgat ccgatgccct
     8941 tgccctggta gtggtcactg atgttgaagg cgacctcggc gctgcgggcg tcgatgcggt
     9001 cgtagcgacc gatcccgacg atgtcctcgc gcagcgtgac gaccagggcg acccggtcga
     9061 cgtagtccac atgggtgaag cggtggacgt cggcgtcgct cagccgccgc agcggggcga
     9121 agaatcgcag gtagatggac tcgtcggact ggccgctgtg gaaacggtgg atgccgtcgg
     9181 tgtccgaggg tcggatcggc cggacgtggg cgacggtgcc gtcgcgcagg acgacgtcgg
     9241 cctcccactc gctggggtaa cccggaggaa gcgggtcgtc gtccatgggc accatcgtgc
     9301 caccacgagg gacccgtcgg acacactccc ggcacccacc ggcacactgg ggtcgtggag
     9361 ctacgcgacg cagtgctgcg gcggcggatg gtgcgccggt tcgacccgtc cgtcccggtc
     9421 gagatggagg tcgtgcgcga cctggtcgcg ctcgcggtgc gggccccgag cgccgggttc
     9481 agccaggggt gggacttcgt cgcgctgctg aacccgcagg accgcgcggc cttctgggcc
     9541 gctgccgacg acgggggccc ggccgatgcg tggctacgcg gggtgtccgc cgctcccgcc
     9601 ctggtgctgt gcctgtccga tgccggcgcc tacctcgacc ggtacgcgca ggcggacaag
     9661 gggtgggtcg accgctcccc cgatcgctgg ccgatcccct actgggacac cgacaccgcc
     9721 atggcggcga tgatcatcct cctgggcgca caggatgccg ggctgggcgc cttgttcttc
     9781 ggcatcccgg cccagcgcca cgacgcggtt cgtgaggcgc acggcatccc cgaggaccgg
     9841 cgcctggtcg gggtcatcgc cctgggcacc ccggccgctc gggccgcgca caccggcggg
     9901 tcaccgcgca cgaggccacg tcgccccctg gaggacgtgc tgcacgcggg acggttcggc
     9961 gggtggtccg gcgcgtcggc gtgaacggcc gctgaccgat gggtgaggat gacggggtcc
    10021 cctgccgcgg catccgcggt ccgagccctc acgtagagca gagcccgacc ccatggccaa
    10081 gcgcaccacc accccaccgc ccggggagac cccgcaggac ggcgtcgagc gggtcgtcga
    10141 catcgacgtc gaggacgaga tgcgcagtgc gttcctcgag tactcctaca gcgtcatcta
    10201 ctcccgtgcc ctgcccgatg cgcgcgacgg gctcaagccc gtgcagcgcc gcatcctgtt
    10261 cgggatgagc gagctgggcc tgcgccctga ccggccccac gtgaagtcca gccgggtcgt
    10321 cggtgaggtc atgggcaagt accaccccca cggcgaccag gcgatctacg acgccctggt
    10381 gcgcatggcg cagccgttca cgatgcgcct gccgctcgtc gacggccacg gcaacttcgg
    10441 ctccctcgac gacggcccgg ccgcctcgcg gtacaccgag gcccggatgg ccccggcagc
    10501 actgctcatg gtgtccgggc tcgacgagga caccgtcgac tacgtcccca actacgacga
    10561 ctcgctcctg cagcccggcg tcatgcccgc tgcctacccc aacctgctcg tcaacggcgc
    10621 gtccgggatc gccgtcggta tggccacgaa catggccccg cacaacctcg tcgaggtcat
    10681 cggtgccgcc cgccacctca tcgcccaccc ggactgttcc ctggacgacc tgatgaagtt
    10741 cgtcccgggc cctgacctgc cgggcggcgg gcggatcgtg gggctcgacg gcatccgcga
    10801 cgcctacctc accgggcggg gctcgttccg cactcgcgcc gccgcacgcg tggagaagct
    10861 caccccgcgg cggatgggca tcgtcgtcac cgagctgccc tacctcgtcg gcccggagaa
    10921 ggtcatcgag cgggtgaaga ccctggtgca ggccaagaag ctccagggca tctccgacgt
    10981 caaggacctc accgaccgca agaacggcct gcggctggtc ttcgaggtca agaacggctt
    11041 caaccccgac gccgtgctcg agcagctgta caagctgacc ccgatggagg actcgttcgg
    11101 gatcaacaac gtcgccctcg tcgacgggca accccgcacc ctgggcctga aggagctgct
    11161 gcgcacctac gtcgacttcc gcatcgaggt cgtgcgccgc cgcaccgagt tccggctgcg
    11221 caagcgccag gaccgactgc acctcgtcga gggcctgctc atcgccatcg tcgacatcga
    11281 cgaggtgatc cagctgatcc gcacctccga cgacgcggcg accgccaagg cacggttgat
    11341 gtccgtcttc gacctctccg aggcccaggc cgagtacatc ctcgagctcc agctgcgccg
    11401 gctgacgaag ttctccctca tcgagctcga gagcgagcgc gacgagctgc gccgccagat
    11461 cgaggagctc gaggcgatcc tcgccgacga gaagctgctg caccgcaccg tctccggcga
    11521 gctcgccgac gtcgccaagg cccacggcac cccacgacgg accgtgctgc tggagtcggc
    11581 gggggtgccc gcatcggtgg gagcgccgct cgaggtggcc gacgacccgt gctgggtgct
    11641 gttgtcgtcc accggcctgc tggcgcgcac gtcctcggcc gacccggtgc ccgctgacgg
    11701 tggccgcgcc aagcacgacg ccatcatcgg tgccgtgcgc accacggccc gcggcgagtt
    11761 cggcctcgtc acctcggcag ggcggatgct gcggatgtcg gccctcgagc tgccgacgct
    11821 ccccccgctc gggggcgcgc ccagcctgtc cggcggtgct ccgctcgcgg tcttcgccga
    11881 cctgcccgcc ggggaggaac cgctgaccat cgtcgggttg gatgccgagg ggccggggat
    11941 cgcgctcggc accgcccacg gtgtcgtcaa gcgcgtcacc cccgaccacc ccgctcgggc
    12001 gaactgggag gtcatcgccc tcaaggacgg tgaccgggtc gtcggtgcgg ccgccctgcg
    12061 cgacggcgag gaggatctcg tcttcgtcac gagtgacgcg cagctgctgc gcttctcggc
    12121 gtcgtcggtg cggccccagg gccgagcggc cggtgggatg gccggcatcc gcctcaaccc
    12181 cggcgcctcc gtcgtcttct tcggtgccgt cgacccgacg gcggatgccg tcgtcgtcac
    12241 ctcctcgggc gcctccgacg cgctgcccgg cacgcagccc ggtgccatca aggtgacccc
    12301 gtacgccgag tacccaccga agggtcgcgg gacgggcggg gtgcgagcac accggttcct
    12361 gcgtggcgag gacgccctga tcctggcctg ggtcggtgcc caaccgccgc gggccagcgg
    12421 tgcggccggc gtggcgctcg agcttccgga ggcgtccggc aggcgagacg gctccggcac
    12481 ggcctatccg gccgtcatcg gcgggatcgg ctcaccggtt cgctgaggtc ccccgccctc
    12541 aggcggagat cgcgctctct cgccggcgga tcgaaccgtc cgccacctca gcgttgccgc
    12601 cgtgttacgc aatcgttacc gcctcgatgc gtggagacgt tgcgaagcat gtgtaaacgc
    12661 ttttaccttg gtcccggttc cctcatcgcc gaggccacag cgtctcggcg cgaccagtga
    12721 aatggaggcg ggcacatggc cctctacacc aagcgtcgca tcgcggcgtt gtccggagtc
    12781 ctcgcggtgg cactcaccgc gacggcctgc ggaagcagca gcgacgacgg tggcgacacc
    12841 gccgaaagcg gcagcaccag cagcatcgac tgcgcgccct acacggcgtt cggcgacctc
    12901 aagggcaaga cggtcaccgt ctacacctcg atcaccgctc ccgaggacgc accgcacatc
    12961 gcgtcctacc agcccttcgt ggactgcacg ggcgtcgaga tcaagtacga aggctccaag
    13021 gagttcgagg cccagctgcc ggtgcgtgtc aagggtggca acgcccccga catcgcctac
    13081 ctgccccagc cgggtctgct caacaccctc gtccaggcgg gcgccgtcaa ggccgccccg
    13141 ccggaggtca tcgccaacgt cgacaagaac atgccggact ggaagaacta cggcaccgtc
    13201 gacggcacct tctacgccgc gccgctcggt gcgaacgtga agtccttcgt ctggtactcg
    13261 ccgtcggcct tcgccgacaa gggctacgag gtccccacca cgtgggccga cctgctcgcc
    13321 ctgtccgaga agatggccgc cgagggtggc gacgtcaagc cctggtgtgc aggcatcgag
    13381 tccggtgacg ccaccggctg gccggccacc gactgggtcg aggacgtcat gctccgcacc
    13441 gccggcgccg agacctacga caagtgggtc aaccacgaga tcccgttcaa cgacccggcc
    13501 gtcgccacgg cgctcgccga ggtcggcaag atcctcaaga acgacaagtt cgtcaacggt
    13561 ggcttcggcg acgtcaagac gatcgcctcg acgtccttcc aggacgccgg tcagccgatc
    13621 ctcgacggca cctgctacat gcaccgtcag gcgtcgttct acgcggccaa ctggcccgag
    13681 ggcaccgagg tcggtcccga gggtgaggtc tgggcgttct acctgccctc cgtcgactcc
    13741 tccaccaagc cggtcctcgg tggtggcgag ttcaccgcgg ccttcgccga ccgtcccgag
    13801 gtcaaggcct tccagaccta cctctcctcg gtcgagtggg ctgacgagcg cgccaagacc
    13861 tgcggttcgg gtggttgcgt gacggccaac aagaacgccg acccctcgct gctgaagaac
    13921 cccgtcgaca agctctctgc cgaggcgctc accgacagtg cagccacctt ccgcttcgac
    13981 ggttccgacc tcatgccggg tgccgtgggt gcgggtacgt tctggaaggg catgaccgac
    14041 tggatcatcg gcaaggacga caaggcaacg ctcgacttca tcgagcagtc ctggccgaag
    14101 tgacaaccct gacctgattc accccaggtc aacgagcagg ggtggggtgg gactccccca
    14161 ccccacccct gctctcttgg tttcccctgt cctgacgtaa tcctctcgga ggtgatgtcc
    14221 agtggtatcg acggtcatcg gcgcagttcc cgccgcgatt cccgagccgt tcctgcgggc
    14281 gcaaagcgtg cccggcaagt tcttcctgat gttcttcgcg atcttcttct tcgcgctcgt
    14341 gttcggcctc gtgctcttcc tcgcctccct gttcaagggc aagaacggcg agcgggtcca
    14401 gggcgccctg ttcgtcggcc cagccgtggt cctgctcgcg atcggcctga tgtaccccgc
    14461 gatcctcacg atcaaccagt cgctgcgcgg gcgctccggt gagggtggac tcaccctcag
    14521 caactactcg gcgatgttca cccagcccga gctgctgctc gtgctgcgca acacggccct
    14581 gtgggtcatc ctcgtcccga tcctcgcgac cagcatcggg ctgatctacg cgatcctcat
    14641 cgaccgcgcg cgcggcgagg ccttcgccaa ggccctgatc ttcctgccga tggcgatctc
    14701 gatggtcggc gccggcatca tctggaagtt cgtctaccag tacaagcaga cgtcccgccc
    14761 gcagatcggc ctgctcaacg cgatcctgaa gctgctcggg ttcgagaccc agcagttcct
    14821 catcaacgcg ccgctcaaca ccttcatgtt gatcatcgtc atggtctgga tccaggccgg
    14881 gttcgcgatg acggtcctct ccgccgccat caaggccatc cccgacgaga tcatcgaggc
    14941 cgccaagctc gatggcgtcc aggcgttcaa gatgttccgc tacatcacgc tgccgagcat
    15001 ccgggccgcc ctcgtcgtcg tcgtgacgac gatcggcatc gggacgctca aggtgttcga
    15061 catcgtgcgc accatgaccg gtgggcagtt cgacacctcg gtcgtcgcca acgagttcta
    15121 cagccagagc ttccgcttca acgctccggg actcggcgcg gccctcgcgg tgctgctgtt
    15181 cattctcgtc atcccgatcg tgacgtacaa catcatccag atgcgaaagg aggcctgagc
    15241 catgagcacc ctcgaaccgg tggccgtgcc
//
LOCUS       DAOSOC010000043        15166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3940_length_15166_cov_10.002515, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000043 DAOSOC010000000
VERSION     DAOSOC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 15166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15166
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3940_length_15166_cov_10.002515"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4567)
                     /gene="dnaE"
                     /locus_tag="PL014_05225"
     CDS             complement(<1..4567)
                     /gene="dnaE"
                     /locus_tag="PL014_05225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT56525.1"
                     /translation="MSSANDSFVHLHVHTEYSMLDGAARIGDLFERAAEMGMPALATT
                     DHGYLFGAYEFWKKAQGTGVKPIIGLEAYVTPGTHRTDKTRVKWGDERSRPGDDVSGS
                     GAYTHMTLLARDNVGLHNLFRMDSQASLDQVWAKWPRIDRELLDQYGKGLIATTGCPS
                     GEVQTRLRLGQYDQAKQAASDLRDLFGAENFFCELMDHGLDIERRVQKDLLRLARELG
                     LPLVATNDLHYTSPEDAKAHSALLCVQSGSTMMDPNRFKFDADDFYLKSPAEMRHTWR
                     ELPEACDNTLLIAEMCDISFTVDEGRYMPQFPCPPGEDEASWFVKEVQRGLHERFPAG
                     IPDYAQKQAEFETDVIVSKGYCFPPETPVLMGDGGWKAISDVVEGDVVTSVDTHALRG
                     RNAIVEKVLTRSVSEDLHVIHPYGQAFPLKATAEHPFLVAHRGWVEAKDVRPGDEVLL
                     PQPERAVYQDLDVVALMEAAGWTIGQSPDGRVHRMGAGLKRAARPNVGTLPSKLEWST
                     DLLWLFGLWVAEGHLYDPEGGVNPGQIGWTLHEDEPAVQRLCRVIDDLDLGTARVYSK
                     ASPSVPGHHGVVVMLTNHPLALVLTRLFGSGVRNKRVPEWVLSTPDEGLRAFVRGWHE
                     GDGHVDARGKWMVTTVNDVLARQLRSILLSLGQWCTLQRSPSQRSYKVLWVPQSQKQP
                     YNARCVDGRWWVRVRAVSREAYSGTVFNLTVSGDHTYVAEGVAVHNCGYFLVVADFIT
                     WAKNNGIRVGPGRGSGAGSMCAYAMKITDLDPVPHGLIFERFLNPERPSLPDFDVDFD
                     ERRRGEVIRYVTEKYGSERVAQIVTYGTIKAKQAVKDAARVMGHPFAVGEQLTKAMPP
                     DIMGKGVPLSKMYDETHDRYGEGKEFRDLVAGEPHLQEVVETAKGLEGLKRQWGVHAA
                     GVIMSSEPLIDVIPIMRRLQDGQVITQFDYPSCESLGLVKMDFLGLRNLTILDDAIAN
                     VQSNRGEEIDLDALSKDMTDRATFDLLARGDTLGVFQLDGGGMRTLLKLMQPDNFEDI
                     SAALALYRPGPMGVNAHTNFALRKNGKQALIPLDPQLEGKLQPEMVSALEPILGTTYG
                     LVIYQEQVMEIAQKLAGYSLGNADLLRRAMGKKKKEVLDAEYVPFSEGMKAKGFNEAS
                     IAALWGVLVPFSDYAFNKAHTAAYGLVSYWTAYLKANYPAEYMAALLTSVGDDKDKSA
                     LYLGECRHMGIKVLPPDVNDSLGQFAAVGADIRFGLQAIRNVGHNVVEAIIAARAEKG
                     PFTSFRDFMSKVPAVVCNKRTIESLIKGGAFDGLGEPRMGLLQVHEEYVDAFVAVKRQ
                     EAIGQDSLFGSFGDDSGGDSGGDLMGLASVPAVEWDKSALLSFEREMLGLYVSDHPLF
                     GVEHVLSQHADTQIAALTADEAGRPEGSMVTIAGLITGLQVKRTKKGDLWAIATVEDL
                     AGSIECLFFPSAYMTVGPMLRQDTVAVVRGKINRRDDTVSIYAQELTLPDVSEGPRGP
                     VVVTMETARATTERVEELKSVLTNHPGT"
     gene            complement(4635..5558)
                     /locus_tag="PL014_05230"
     CDS             complement(4635..5558)
                     /locus_tag="PL014_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT56526.1"
                     /translation="MTDARTVLVPDGLEGERVDAAIARLFGLSRTRAADLAAEGGVVV
                     NTASVGKSHRVSAGDVLEVTLPASPVSPTLEVVAEPVPGMTVIHDDDDIVVVDKPVGV
                     AAHPSVGWSGPNVLAGLKAAGYRISTSGASERQGIVSRLDVGTSGLMVVCKSERGYSV
                     LKRAFKERRVDKTYHTLVQGLPDPLEGTIDAPIGRHPGHDYKFAVMDSGKHAVTHYEL
                     LEAFRYASLLEIKLETGRTHQIRVHMAAVRHPCVGDLTYGADPTLAQRLGLERQWLHA
                     VGLGFEHPGSGEWVTFTSSYPADLQGALDAL"
     gene            complement(5551..6042)
                     /gene="lspA"
                     /locus_tag="PL014_05235"
     CDS             complement(5551..6042)
                     /gene="lspA"
                     /locus_tag="PL014_05235"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT56527.1"
                     /translation="MTTLVLGLALAVLVVDQGTKVWALNTLTPGEKIDLIGSLISLNL
                     IRNPGAAFSIGDGATWILTIVSLAILGWVLVAARKVGTRAWAVALGLLLGGALGNLGD
                     RFFREPGPGRGHVVDFIDYFGWFIGNVADIAIVVGAGVIMVLAYRGTPVSGPPHHPEH
                     AHD"
     gene            complement(6102..6539)
                     /locus_tag="PL014_05240"
     CDS             complement(6102..6539)
                     /locus_tag="PL014_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOT56528.1"
                     /translation="MVARKTPTAKKATSSRTLAVREDESPWTAKELRDLRGELEQEIA
                     QLRSELLEAESDLVDLMRDSGDGAGDDQADAGAKTYEREQEISLANNARLMLEQSEHA
                     LERFDGGTYGICESCGDPIGKLRLQAAPRATLCMPCKKKQERR"
     gene            complement(6711..8216)
                     /locus_tag="PL014_05245"
     CDS             complement(6711..8216)
                     /locus_tag="PL014_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT56529.1"
                     /translation="MALTPEDVLNKTFTQTQFRRGYDEREVDDFLDDIVAEMRRMTKE
                     SDDLRGELNAVREGNGLAPVAARQDPDTSAGDLDELRSKNATLAARVSELESTRSDLT
                     ARLGEYEGKSGDTEARNAELQGQLAQLQHRGGELEAQAAEATARADELEAANAALQER
                     ATELETKVAQLEGELEASRNDLAAVQAEREQDAAARQEAAARQDAAAAEDNDQVAALR
                     ARIDEVEQDARARIAEATARAEEAEQRAAAAAQRGDGDGAVGAVALAGAGGGASAAGV
                     IALAQRLHDEHVAAGEARHEELVTEGQRRHDELVSTGQARHDELIGTAQSRHDELIST
                     GQARHDELISTAQAKHDELISTGQAQHDEMIATGTSTKDELISTATAQRDEMIATATA
                     QRDEMIATATAQRDELLTEGRERSTGMVAEAQQKKAGILEELGRERGLLERKIEELSG
                     FERDYRSRLHAYIQGQLADLKNTGVDHSDEESGDAEQHEGAAAEPQPEHQG"
     gene            complement(8360..8635)
                     /locus_tag="PL014_05250"
     CDS             complement(8360..8635)
                     /locus_tag="PL014_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOT56530.1"
                     /translation="MNAVREVLGFVVFLYFLVLLGRVVFDWIRIFARDWRPRGAALLV
                     AEPIYTLTEPPLRALRKVIPPLRLGAVNLDLSFMVLFFVVYLLMILL"
     gene            complement(8682..9179)
                     /gene="sepF"
                     /locus_tag="PL014_05255"
     CDS             complement(8682..9179)
                     /gene="sepF"
                     /locus_tag="PL014_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOT56531.1"
                     /translation="MAGALRKTMVYLGLAEDQGRYDDDYDEMYDDHVDPVEDHAAPER
                     AERRSAEVTHLPTRHSVAHVVSSPEVGGLSRITTIHPRTYNEAKTVGENFRDGIPVIM
                     NLTDMSDADAKRLVDFAAGLVFGLRGSIERVTSKVFLLSPETVEVNAEEGPIAASGRG
                     VFNQS"
     gene            complement(9236..9991)
                     /locus_tag="PL014_05260"
     CDS             complement(9236..9991)
                     /locus_tag="PL014_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010143377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT56532.1"
                     /translation="MITPEPPRPIDPAGPGSLPDLEQRRRELELNLSAVHDRIERARH
                     DAGRTDELTLVVVTKFFPASDVDLLAGLGVTDIGENRDQEASAKLESVAHRSGLRVHF
                     IGQLQSNKAGSVARYADVVQSVDRAKVVRALDRGASAVGRVLDVTVQVALADGEGRGG
                     VPVDQVEALADTVAGSTSLRLRGVMAVAPLGADPHPAFARLREVSDGIRASHPDATWI
                     SAGMSGDLEAAVAHGATHLRVGSAILGSRLSHR"
     gene            complement(9988..10692)
                     /locus_tag="PL014_05265"
     CDS             complement(9988..10692)
                     /locus_tag="PL014_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOT56533.1"
                     /translation="MFAWHEDRSGVVRAVTDRSSGVSTGAYAGLNLGGHVGDDPEAVS
                     RNRDALAERLGRPVAYMDQCHGAGVAVIDSVPEVAPTCDALVTASPDLALAVLVADCV
                     PVLLAGEGVVAAVHAGRPGLVAGVVPAAVAAMRDLGADTIDAVVGPSVCGRCYEVPEG
                     MRSDIAAVVPVAATVSWSGTPAVDVAAGVVTQLREAGVTVRWVPGCTRERPDLYSYRR
                     DASTGRFAGVVARSHP"
     gene            complement(10781..11992)
                     /gene="ftsZ"
                     /locus_tag="PL014_05270"
     CDS             complement(10781..11992)
                     /gene="ftsZ"
                     /locus_tag="PL014_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT56534.1"
                     /translation="MAAPQNYLAVIKVVGIGGGGVNAINRMIEVGLKGVEFIAVNTDA
                     QALLMSDADVKLDVGRELTRGLGAGADPEVGKKAAEDHAEEIEEVLRGADMVFVTAGE
                     GGGTGTGGAPVVARIARGLGALTIGVVTRPFTFEGRRRANQAETGIAQLREEVDTLIV
                     IPNDRLLSISDRGVSMLDAFRSADQVLLSGVQGITDLITTPGLINLDFADVKSVMQGA
                     GSALMGIGSARGEDRAVQAAELAISSPLLEASIDGAHGVLLSIQGGSDLGLFEINEAA
                     RLVQEAAHPEANIIFGAVIDDALGDEVRVTVIAAGFDGGAPVRRADDRALGSVQGRSA
                     PAAAAPVAAPAAQRQPAAPVETAPQEGAVMTEHAQAPVEPVRPAQPVQAPPRTVTFDE
                     NDDLDVPDFLK"
     gene            complement(12217..12969)
                     /locus_tag="PL014_05275"
     CDS             complement(12217..12969)
                     /locus_tag="PL014_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOT56535.1"
                     /translation="MSLRTEYPSGAAASSSAARFRERELANRRRPWRRAFAVLVAVTV
                     VAVLVWVLGWSSILGVSKVEVSGATGAEASAVAELVDVPIGTPLARVDTDAAAARVRE
                     RITVAEVSVRRAWPSTLAVDIVLRTPAIVVRNPQGQLEVADAEGVSFGVVKAAPKGVP
                     VVKAVGAKGATREALQSALALLNALPSDLSSQVSGITVSSANLVTFTLGKRTVVWGSG
                     ADSERKVAILTALLPTKAKVIDVSAPETPVTR"
     gene            complement(12966..14444)
                     /gene="murC"
                     /locus_tag="PL014_05280"
     CDS             complement(12966..14444)
                     /gene="murC"
                     /locus_tag="PL014_05280"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT56536.1"
                     /translation="MRFDVTAPATPLADLGRVHLIAIGGAGMSAVARLLLARGVSVSG
                     SDAADGPALQALRTLGARVHLGHDAGHLAGVDTVVVSSAIREDNVELAAARAAGLRVL
                     HRSQALASLMAGSRRVAVAGANGKTTTTSMLVVALDAAGAHPSYACGAEIAQLATNAA
                     IGDGSVFVVEADESDGSFLAYRPDVAVVTNVQPDHLDFYGTAERVEEAYAAFVATVPA
                     DGLVVACFDDPGSRRLAQAVRSCGGAVVTYGYEPGADLRVLSTEPDGLGTHSRFVLDG
                     VEHSLVLLVPGPHNVMDACAAYLAAVHGVGAQPDAVLAGLASFSGARRRFEVRGEAGG
                     VTVVDDYAHNAPKVAAVVSTGADLVRRAGSGALHVIFQPHLYSRTRDFAVEFAHALAP
                     ADHVVLLDVYGAREAPVDGISSALIGDPLRELQGERSVLVGPGREDAVAAVARSARPG
                     DLVLVVGAGDVTGLAPLLVQALSRAASATSTDSSTTSDGGAR"
     gene            complement(14456..>15166)
                     /locus_tag="PL014_05285"
     CDS             complement(14456..>15166)
                     /locus_tag="PL014_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT56537.1"
                     /translation="NRLGARIASAVGVTFPDTPLPRATVTGMPLRREIATLDRGALRE
                     QARAHFGLSDAPTVLVTGGSLGAQRLNDAFAAAAARLSRAGVQVLHVAGTGKDFVPEG
                     VTGAPYVVLAYCDRMDFAYAAADLVVARSGANTVCELTAVGLPAIYVPLPIGNGEQRL
                     NAANVIAAGGGLLVEDSAVTESWVDDVVRPLALDGPRLGVMASASASVGERGGDELLA
                     DLVMAAAAGATTTAGHAR"
BASE COUNT         2242 a         5267 c         5348 g         2309 t
ORIGIN      
        1 tggtgcccgg gtggttggtc agcacgctct tgagctcctc gacccgttcg gtcgtggccc
       61 tggcggtctc catcgtcacg acgacggggc cgcgcggccc ctcgctgaca tcgggcaggg
      121 tcagctcctg cgcatagatg gagacggtgt cgtcacgccg gttgatcttg ccgcgcacca
      181 ccgccacggt gtcctggcgc agcatcggac cgacggtcat gtaggcgctg gggaagaaca
      241 ggcactcgat cgagcccgcg aggtcctcga cggtcgcgat cgcccacagg tcgcccttct
      301 tcgtgcgctt gacctgaaga ccggtgatca ggccggcgat ggtgaccatc gagccctcgg
      361 gtcgaccggc ctcgtcagca gtcaacgccg cgatctgcgt gtcggcatgc tggctcagca
      421 cgtgctcgac cccgaacagc gggtggtcgg agacgtagag gcccaacatc tcgcgctcga
      481 aggacagcag cgccgacttg tcccactcga cggcgggcac gctcgcgagc cccatgaggt
      541 caccgccgct gtcgccaccg gagtcgtcac cgaacgagcc gaacagactg tcctggccga
      601 tcgcctcctg gcgcttgacc gcgacgaacg cgtcgacgta ctcctcgtgg acctgcagca
      661 accccatccg gggctcgccg aggccgtcga acgcgccgcc cttgatgagc gactcgatgg
      721 tgcgcttgtt gcacaccacc gccggcacct tggacatgaa gtcccggaag gaggtgaagg
      781 gccccttctc cgctcgtgcc gcgatgatcg cctcgacgac gttgtggccg acgttgcgga
      841 tcgcctgcag gccgaagcgg atgtcggcgc ccaccgccgc gaactgcccc aaggagtcgt
      901 tgacgtcggg gggcagcacc ttgatgccca tgtggcggca ctcaccgagg tagagggccg
      961 acttgtcctt gtcgtcgccg accgaagtga gcagggccgc catgtactcg gcggggtagt
     1021 tcgccttgag gtacgcggtc cagtacgaga ccaggccgta ggcggcggtg tgggccttgt
     1081 tgaaggcgta gtcggagaag gggacgagca caccccacag ggccgcgatc gacgcctcgt
     1141 tgaagccctt ggccttcatg ccctcggaga agggcacgta ctcggcgtcg agcacctcct
     1201 tcttcttctt gcccatcgct cgccggagca ggtcggcgtt gccgagggag tagcccgcga
     1261 gcttctgcgc gatctccatg acctgctcct ggtagatcac caggccgtag gtcgtgccga
     1321 ggatcggctc gagcgcggac accatctcgg gctggagctt gccctcgagc tgggggtcga
     1381 gcgggatgag cgcctgcttg ccgttcttgc gcagggcgaa gttggtgtgg gcgttcacac
     1441 ccatcggccc ggggcggtag agcgcgagcg ccgccgagat gtcctcgaag ttgtccggct
     1501 gcatgagctt gagcagggtg cgcatgccgc cgccgtcgag ctggaacacc ccgagggtgt
     1561 ccccgcgggc cagcaggtcg aaggtggcgc ggtcggtcat gtccttggac agcgcgtcga
     1621 ggtcgatctc ctcgccacgg ttggactgca cgttggcgat ggcgtcgtcg aggatggtga
     1681 ggttgcgcag gccgaggaag tccatcttga ccaggccgag agactcacag ctcgggtagt
     1741 cgaactgggt gatgacctgc ccgtcctgaa ggcggcgcat gatcgggatg acgtcgatga
     1801 gcggctcgct gctcatgatg acgccggccg cgtgcactcc ccactggcgc ttgaggccct
     1861 cgaggccctt ggcggtctcg acgacctcct gcaggtgcgg ctcgccggcg acgaggtcgc
     1921 ggaactcctt gccctcgccg tagcggtcgt gggtctcgtc gtacatcttc gacaggggca
     1981 cgcccttgcc catgatgtcc gggggcatcg ccttggtcag ctgctcgccg accgcgaagg
     2041 ggtggcccat cacccgggcg gcgtccttga cggcctgctt ggccttgatc gtgccgtacg
     2101 tgacgatctg cgcgacccgc tcgctgccgt acttctcggt gacgtagcgg atcacctcac
     2161 cgcgccggcg ctcgtcgaag tcgacgtcga agtcgggcag cgaggggcgc tcggggttga
     2221 ggaaccgctc gaagatcagg ccgtggggca ccgggtcgag gtcggtgatc ttcatcgcgt
     2281 acgcacacat cgaccccgca cccgagccac gacccgggcc gacccggatg ccgttgttct
     2341 tggcccaggt gatgaagtcg gcgacgacca ggaagtagcc gcagttgtgc accgcgacgc
     2401 cctcggcgac gtaggtgtgg tcaccggaca ccgtgaggtt gaacaccgtc cccgagtacg
     2461 cctcccgaga caccgctcgc acccgaaccc accatcgacc gtccacgcaa cgggcgttgt
     2521 agggctgctt ctgcgactgc ggcacccaca gcaccttgta ggaccgttga ctgggagagc
     2581 gctgcagcgt gcaccactga ccgagtgaca acaggatgga ccgcagctgc cgcgccagga
     2641 cgtcgttgac ggtggtcacc atccacttgc cgcgagcgtc gacatgaccg tcaccctcgt
     2701 gccagcccct cacgaatgcc cgcagccctt cgtccggggt cgagagcacc cattcgggga
     2761 cgcgcttgtt gcggacccct gagccgaaga gcctcgtcag cacgagcgcc aacgggtggt
     2821 tcgtcagcat gacgacgacc ccgtgatgcc ccggcacgga cggcgaggct ttcgaataca
     2881 cgcgcgcggt gccgaggtcg aggtcgtcga tgacgcggca gagtcgctgc acggcgggct
     2941 cgtcctcgtg cagcgtccac ccgatctgtc ccgggttgac gcctccctcc gggtcgtaga
     3001 gatgcccctc cgcaacccac aacccgaaca accacagcag atccgtggac cactccagtt
     3061 tcgagggcag ggtgccgacg ttcgggcggg ccgcacgctt gagacctgcc cccatgcggt
     3121 ggactcgacc gtccggcgac tgtccgatgg tccacccagc agcctccatc agggccacga
     3181 cgtcgaggtc ctggtagacc gcgcgctctg gctgaggcag gagcacctcg tcgccgggcc
     3241 gcacgtcctt ggcctccacc cacccgcggt gagccaccag gaaggggtgc tcggcagtgg
     3301 ccttgagcgg aaaggcctga ccgtacgggt ggatgacgtg caggtcctcc gacaccgaac
     3361 gtgtcaggac cttctcgacg atcgcgttgc gtccgcgcag ggcgtgggtg tcgaccgagg
     3421 tcacgacgtc gccctccacg acatcgctga tcgccttcca cccaccatca cccatgagaa
     3481 ccggtgtctc gggcgggaag cagtacccct tggagacgat gacgtccgtc tcgaactcag
     3541 cctgcttctg ggcgtagtcg gggatgccgg cggggaagcg ttcgtgcagc ccgcgctgca
     3601 cctccttgac gaaccagctc gcctcgtcct cccccggcgg gcacgggaac tggggcatgt
     3661 agcgcccctc gtcgaccgtg aacgagatgt cacacatctc ggcgatcagc agcgtgttgt
     3721 cgcaggcctc gggcagctca cgccacgtgt ggcgcatctc ggccgggctc ttgaggtaga
     3781 agtcgtcggc gtcgaacttg aaccggttcg ggtccatcat cgtcgatccc gactgcacgc
     3841 acagcagggc cgagtgggcc ttggcgtcct cggggctcgt gtagtgcagg tcgttcgtcg
     3901 ccaccagggg cagaccgagc tcacgggcca gccgcaggag gtccttctgc acgcggcgct
     3961 cgatgtcgag gccgtgatcc atcagctcac agaagaagtt ctcggccccg aacaggtcac
     4021 gcaggtcgga tgccgcctgc ttggcctggt cgtactggcc cagtcgcagc cgcgtctgga
     4081 cctcaccgga cgggcagccg gtggtcgcga tgaggccctt gccgtactgg tcgagcagtt
     4141 cgcggtcgat gcgcggccac ttcgcccaga cctggtccag cgaggcctgc gagtccatcc
     4201 ggaacaggtt gtgcagcccg acgttgtccc tggccagcag ggtcatgtgc gtgtacgcgc
     4261 ccgaccccga gacgtcgtcg ccggggcggg agcgctcgtc gccccacttg acgcgggtct
     4321 tgtccgtgcg gtgggtgccg ggagtgacgt acgcctccag cccgatgatc ggcttgacgc
     4381 ccgtgccctg ggccttcttc cagaactcgt aggccccgaa gaggtacccg tggtcggtcg
     4441 tggcgagtgc cggcatcccc atctcggcgg cacgctcgaa gaggtcaccg atgcgcgccg
     4501 caccgtcgag catcgagtac tcggtgtgga cgtgcaggtg gacgaaggaa tcgttcgcgg
     4561 aggacatcgc cgaccatcct aggtcgccgg gccgacaccc cttcggcgtg tcgtcgaccg
     4621 tgtcgacgca gcgctcagag cgcgtcgagg gcgccctgca gatcagcggg atagctgctc
     4681 gtgaaggtga cccactcccc cgatccgggg tgctcgaagc cgagcccgac cgcgtgcagc
     4741 cactgccgtt ccaggccgag ccgctgcgcg agggtggggt ccgcaccgta ggtcaggtca
     4801 ccgacgcagg ggtggcgcac ggccgccatg tgcacgcgga tctggtgcgt gcgcccggtc
     4861 tcgagcttga tctcgagcag cgaggcatag cggaacgcct cgagcagctc gtagtgggtg
     4921 accgcgtgct tgcccgagtc catgaccgcg aacttgtagt cgtggccggg gtggcgcccg
     4981 atgggtgcgt cgatggtgcc ctcgagcggg tcaggaaggc cctggacgag cgtgtggtac
     5041 gtcttgtcga cccggcgctc cttgaacgcg cgcttgagca ccgagtaccc gcgctcgctc
     5101 ttgcacacga ccatcaggcc cgacgtgccg acgtcgagcc gggacacgat cccctggcgc
     5161 tcgctggccc cgctcgtgga gatgcggtac ccggcggcct tcagcccggc caggacgttc
     5221 ggcccgctcc agccgacact ggggtgggcc gccacgccca cgggcttgtc gacgaccacg
     5281 atgtcgtcgt cgtcgtggat gacggtcatg ccgggaacgg gttcggcgac gacctcgagg
     5341 gtcgggctga ccggggatgc cgggagcgtc acctccagga cgtcgccggc gctcacccgg
     5401 tggctcttgc ccacgctggc ggtgttgacg acgacgcccc cctcggcggc gaggtcggcc
     5461 gcccgggtgc gggagaggcc gaagagccgc gcgatggcgg cgtcgacccg ttcgccttcg
     5521 aggccgtcgg gaacgaggac ggtacgggca tcagtcatga gcgtgctccg ggtggtgggg
     5581 tgggccggag acgggagtgc cccgataggc caggaccatg atgacgccgg cacccacgac
     5641 gatcgcgatg tccgcgacgt tgccgatgaa ccacccgaag tagtcgatga agtcgacgac
     5701 gtgcccccga ccggggccgg gctcccggaa gaaacggtcc ccgaggttgc ccagggcgcc
     5761 gccgaggagc agcccgagcg cgaccgccca ggccctcgtg ccgaccttgc gcgcggcgac
     5821 gaggacccag ccgaggatcg ccagcgacac gatcgtgagg atccacgtgg cgccgtcgcc
     5881 gatcgagaag gccgccccgg ggttgcggat gaggttcagc gagatcaacg acccgatgag
     5941 gtcgatcttc tcccccggcg tcagcgtgtt cagggcccac accttggtgc cctggtcgac
     6001 gacgaggacc gcgagggcaa gccccagcac aagggtcgtc aaccgtcgct gacgccccgg
     6061 cggaaccggg ggcggcgtgg acggggcggg gtgactcgag gtcagcggcg ttcctgcttc
     6121 ttcttgcatg gcatgcacag ggtcgcacga ggagcggcct ggagcctaag tttgccgatc
     6181 gggtcgccgc acgactcgca gattccgtac gtccctccgt cgaaccgctc gagggcgtgc
     6241 tcactctgct cgagcatcaa gcgggcgttg ttggccagcg agatctcctg ctcgcgctcg
     6301 taggtcttcg ccccggcatc cgcctggtcg tcgccggcgc cgtcaccgga gtcacgcatg
     6361 aggtcgacga ggtcgctctc ggcctccagg agctcgctgc gcagctgggc gatctcctgc
     6421 tcgagttcgc cgcgcaggtc acgaagctcc ttggcggtcc acggcgactc gtcctcgcgc
     6481 acggccaagg tccggctgga cgtggccttc ttcgccgtcg gcgtcttcct ggcaaccatg
     6541 cgtacccact tcctgagggt cggcgggccg tggtcgaccg tcgacagtgg ggtgaggata
     6601 cggcgtgagg ggccttgcct caacgacaaa acgccggcgt ggacgacgag gtcgctcaca
     6661 caacgaggga agggcccggc cgatggccgg acccttccct cgtgcgtgcg tcagccctgg
     6721 tgctcgggct gcggctcggc agccgcgccc tcgtgctgct cggcgtcgcc ggactcctcg
     6781 tcggagtggt cgacaccggt gttcttcagg tccgccagct gcccctggat gtaggcgtgc
     6841 aggcgcgagc ggtagtcgcg ctcgaagcca ctcagctcct cgatcttgcg ctcgagcaga
     6901 ccacgctcgc gaccgagttc ctcgaggatc ccggccttct tctgctgggc ctcggccacc
     6961 atgcccgtgg aacgctcgcg cccctcggtg agcagttcgt cacgctgggc ggtggcggtc
     7021 gcgatcatct cgtcacgctg ggccgtcgcg gtcgcgatca tctcgtcacg ctgggccgtc
     7081 gcggtggaga tgagctcgtc cttcgtgctc gtaccggtgg cgatcatctc gtcgtgctgc
     7141 gcctgaccgg tgctgatgag ctcgtcgtgc ttggcctgcg ccgtgctgat gagctcgtcg
     7201 tggcgggcct gaccggtgct gatgagttcg tcgtggcggg actgcgccgt gccgatgagc
     7261 tcgtcgtggc gggcctgacc ggtgctgacc agctcgtcgt gacggcgctg gccctcggtg
     7321 accagctcct cgtgacgggc ctcaccagca gccacgtgct cgtcgtgcag gcgctgggcg
     7381 agggcgatga caccggcggc gctggcgcca ccaccggcac cggccagggc gacggcaccc
     7441 acggcaccgt caccgtcacc gcgctgggcc gctgcggcgg cgcgctgctc ggcctcctcg
     7501 gcgcgggcag tcgcctcggc gatgcgggcc cgggcgtcct gctcgacctc gtcgatgcgg
     7561 gcgcgcagag ccgccacctg gtcgttgtcc tcggcggcag ccgcgtcctg gcgggcagcg
     7621 gcctcctggc gggcggcggc gtcctgctcg cgctcggcct gcacggcggc gaggtcgtta
     7681 cggctggcct cgagctcacc ctcgagctgc gcgaccttgg tctcgagctc ggtggcgcgc
     7741 tcctgaaggg ctgcgttggc ggcctccagc tcgtcggcac gcgcggtggc ctcggcggcc
     7801 tgggcctcga gctcgccacc ccggtgctgg agctgggcga gctgaccctg cagctcggcg
     7861 ttgcgggcct ccgtgtcgcc ggacttgccc tcgtactcgc ccaggcgtgc cgtgaggtcg
     7921 ctgcgggtgc tctcgagctc ggagacacgg gcggcgagcg tggcgttctt cgagcgcagc
     7981 tcgtcgaggt ccccggcgga ggtgtccggg tcctggcggg ccgcgaccgg tgccaacccg
     8041 ttgccctcgc ggacggcgtt gagctcgccg cgcaggtcgt cagactcctt ggtcatccga
     8101 cgcatctcgg cgacgatgtc gtcgaggaag tcgtcgacct cccgctcgtc gtacccacga
     8161 cggaactggg tctgggtgaa cgtcttgttg agaacgtcct cgggcgtgag agccatggtc
     8221 aactcctgtg gtggggcatc ggtgtcgagt cccgctcccg gcatccacgc gcagcgcgac
     8281 accgtgtagg gaccggtctc gccagactac gcgatcaacg gggccaaccg tcatcccaga
     8341 cggcgagcgg accttgccct cacagcagga tcatgagtag gtacaccacg aagaagagca
     8401 ccatgaacga caggtcgagg ttgacggctc cgaggcgcag cggggggatg accttgcgca
     8461 gggcccgcag cggcggctct gtgagggtgt agatcggctc ggcgacgagc aacgcggcac
     8521 cacggggacg ccagtcgcgg gcgaagatcc tgatccagtc gaagacgacc cgcccgagca
     8581 gcacgaggaa gtacaggaag acgacaaagc ccaggacctc acgcaccgcg ttcatggcag
     8641 gagtgtgact gacgaggagg tgatttcccg aaacgttcgc gtcagctctg gttgaacact
     8701 ccgcgaccgg acgcggcgat cggcccttcc tcggcattga cctcgacggt ctcgggcgag
     8761 agcaggaaca ccttggacgt cacccgctcg atcgatccgc gcaggccgaa cacgagaccg
     8821 gcggcgaagt cgacgaggcg cttggcgtcg gcgtcgctca tgtccgtgag gttcatgatc
     8881 accgggatgc cgtcgcggaa gttctcgccg acggtcttgg cctcgttgta ggtgcgcggg
     8941 tggatcgtcg tgatgcggga gagcccgccc acctccgggc tgctcaccac gtgcgccacc
     9001 gagtgacgag tgggcaggtg ggtgacctcg gcacttcgac gctcggcgcg ctcgggggcg
     9061 gcgtggtcct cgaccgggtc gacgtggtcg tcgtacatct cgtcgtagtc gtcgtcgtag
     9121 cgaccctggt cctcggcgag gcccaggtag accatcgtct tgcgcagcgc gcctgccatt
     9181 gagtgctcct tggtcgtcgg ccccgcctga tcggcaccgt ccatcgcgac tgaagttacc
     9241 ggtgtgacag ccgagatccg aggattgccg atccgacacg caggtgtgtc gcgccgtgtg
     9301 ccacggcggc ctcgagatca ccgctcatgc cggcggagat ccacgtggcg tccgggtgag
     9361 atgctcggat gccgtcgctc acctcccgca ggcgggcgaa ggcggggtgc gggtcagccc
     9421 cgagcggggc gaccgccatc acgccgcgaa ggcgcaacga ggtcgagccg gcgacggtgt
     9481 ccgcgagcgc ctcgacctgg tcgacgggca ctcctccccg accctcgccg tcggccaacg
     9541 ccacctgaac cgtcacgtcc aggacccggc ccaccgcgct ggctccccgg tccagggcgc
     9601 gcaccacctt ggcccggtcg accgactgca cgacatccgc gtaccgcgcg accgaccccg
     9661 ccttgttgct ctgcagctgg ccgatgaagt ggacgcgcag accactgcga tgtgccactg
     9721 actccagctt cgcggaggcc tcctggtcgc ggttctcccc gatgtcggtg acgccgagac
     9781 cggccagcag gtcgacgtcc gacgccggga agaacttcgt gacgacgacc agggtcagct
     9841 cgtcggtgcg gccggcgtcg tggcgggccc gctcgatgcg gtcgtggaca gcgctcaggt
     9901 tcagctcgag ctctcggcgt cgctgctcca ggtcgggaag ggagcccgga ccggcgggat
     9961 cgatcgggcg cgggggttca ggcgtgatca cgggtgactc cgcgcgacga ctccggcgaa
    10021 gcggccggtg cttgcgtccc ggcggtacga gtagagatcc gggcgctccc tggtgcagcc
    10081 gggcacccag cgcaccgtga cgccggcctc ccgcaactgc gtcaccacac cagctgccac
    10141 gtcgacggcc ggtgtccccg accacgacac ggtcgccgcc acgggcacca cggcggcgat
    10201 gtccgaacgc atcccctcgg gcacctcgta gcaccgtcca cacacggagg gcccgacgac
    10261 ggcgtcgatc gtgtcggcgc cgaggtcgcg catcgccgcg acggctgccg gaaccacacc
    10321 agccaccagg ccgggtcgcc cggcgtgcac ggccgccacc accccctctc cggccaacag
    10381 cacggggacg cagtcggcga cgagcacggc cagtgccagg tcaggtgacg cggtcacgag
    10441 ggcgtcgcag gtcggggcga cttccgggac gctgtcgatg acggccaccc cggcaccgtg
    10501 gcactggtcc atgtacgcca ccggccgacc caggcgctcg gccagtgcgt cgcggttgcg
    10561 gctcaccgcc tcggggtcgt cgccgacgtg accgccgagg ttcagcccgg catacgcccc
    10621 tgtgctcacg cccgacgagc gatccgtgac agcacggacg acgccgctgc ggtcctcatg
    10681 ccaggcgaac acgctcccac cttaggcgcg cgggccgcag acgtcagaag cgggccggtg
    10741 tgaaccgacc cgcttctcac gtgcagtgct gcgcggagac ctacttgagg aagtcaggca
    10801 cgtcgagatc gtcgttctcg tcgaaggtca ccgtgcgcgg cggcgcctgc acgggctgcg
    10861 ccgggcggac cggctcgacc ggagcctggg cgtgctcggt catgacggca ccctcctgcg
    10921 gggcggtctc gaccggggct gcgggctgac gctgcgcggc gggcgctgcc accggcgccg
    10981 cggccgcggg cgcggaccgt ccctgcaccg agccgagcgc ccggtcgtcg gcgcgccgca
    11041 ccggagcccc gccatcgaac ccggcagcga tgaccgtgac gcgcacctcg tcgccgaggg
    11101 cgtcgtcgat gacggcaccg aagatgatgt tggcctcggg gtgggcggcc tcctgcacca
    11161 gtcgcgccgc ctcgttgatc tcgaacagac cgaggtcgct gccaccctgg atggagagca
    11221 ggacaccgtg ggcgccgtcg atgctcgcct cgagcagtgg gctggagatg gcgagctcgg
    11281 cagcctggac ggcacggtcc tcaccgcggg ccgagccgat gcccatgagc gctgagccgg
    11341 ccccctgcat caccgacttg acgtcggcga agtcgaggtt gatcagaccc ggcgtcgtga
    11401 tgaggtcggt gatgccctgc acaccggaca ggagcacctg gtcggcgctg cggaacgcat
    11461 cgagcatcga caccccgcga tcgctgatgg acagcaggcg gtcgttcggg atgacgatga
    11521 gggtgtcgac ctcttcgcgc agctgggcga tgccggtctc ggcctggttg gcgcggcggc
    11581 gaccctcgaa ggtgaagggg cgcgtgacga cgccgatggt cagggcaccg agaccgcgag
    11641 cgatgcgggc cacgacgggt gcgccacccg tacccgtgcc gccgccctcg ccggcggtga
    11701 cgaagaccat gtcggccccg cggaggacct cctcgatctc ctccgcgtgg tcctccgcag
    11761 ccttcttgcc gacctcgggg tcagcaccgg cgccgagccc gcgggtcagc tcccggccga
    11821 cgtcgagctt gacgtccgcg tcgctcatga gaagcgcctg ggcgtcggtg ttcacggcga
    11881 tgaactcgac ccccttgagg ccgacctcga tcatccggtt gatggcgttg acaccaccgc
    11941 cgccgatgcc gacgaccttg atgacggcga ggtagttctg gggtgctgcc acgatgcggg
    12001 ccttccactg ggtctgaact cgggttcgtt gatcgaaaaa ctctcaacgt gaggttgaca
    12061 gtcacacttt ggtgcggttc tatggcatga cgctaggtcg gtccccgacg tcaggcaaag
    12121 acgccgcggg cgtgtcgcga catcccctgg ggaaatcctc ccacgcgagg actcaggggc
    12181 cacatcagcc ccaccagtca cacgagtaac tccacctcag cgggtcaccg gggtctcggg
    12241 cgcgctcacg tcgatgacct tggccttggt gggcagcagc gcggtgagga tcgccacctt
    12301 gcgctcggag tccgctccac tgccccacac cacggtgcgc ttgccgaggg tgaaggtcac
    12361 caggttggca ctgctcacgg tgatgccgga cacctggctc gagaggtcgg agggcaaggc
    12421 gttcagcagc gcgagcgccg attgcagggc ctcccgagtg gcacccttcg cccccaccgc
    12481 cttcaccacg ggaaccccct tcggggccgc cttgacgaca ccgaaggaga ccccttcggc
    12541 atccgcaacc tcaagctgac cttgagggtt cctcacgacg atggcaggag ttcgcaggac
    12601 gatgtcgacc gcgagcgtgc tcggccaggc tcggcgcacc gacacctcgg ccacggtgat
    12661 ccgctcacga acccgggcgg ccgcggcatc ggtgtccacg cgtgccaacg gggtgccgat
    12721 cgggacgtcg acgagttcgg cgacggcgct ggcttcggcg ccggtcgctc cagagacctc
    12781 gaccttcgag acgccgagga tgctgctcca ccccagcacc cagacgagca cggccacgac
    12841 ggtgacggcc accagaaccg cgaacgcgcg ccgccacggg cggcgccggt tggcgagctc
    12901 acgctctcgg aaccgcgcgg cggaggagga tgccgcggct cccgacgggt actcggtgcg
    12961 cagactcatc gggcccctcc gtcgctggtc gtgctgctgt cggtcgacgt ggcactcgcg
    13021 gcgcgtgaca gggcctgcac caggagtggc gccagcccgg tgacgtcccc ggccccgacg
    13081 acgaggacga ggtccccggg ccgggcgctg cgcgcgaccg ccgccacggc atcctcgcga
    13141 ccagggccga cgagcacgct ccgctccccc tgcagctcgc gcagcgggtc cccgatgagg
    13201 gccgagctga tcccgtcgac gggggcctcg cgcgcgccgt agacgtcaag cagcaccacg
    13261 tggtcggcgg gagccagcgc gtgggcgaac tcgacggcga agtcccgcgt gcgggagtac
    13321 aggtgcggct ggaagatcac gtgcagcgcc ccggatcctg cgcggcgcac gaggtctgcc
    13381 ccggtgctga ccaccgccgc caccttcggt gcgttgtggg cgtagtcgtc gacgaccgtg
    13441 acgccacccg cctcaccacg gacctcgaag cgacggcggg cgcccgagaa gctcgcgagg
    13501 ccggccagca cggcatccgg ctgcgcgccc actccgtgca ccgcggcgag gtaggcggcg
    13561 caggcgtcca tcacgttgtg cggtcccggg acgaggagga ccagggagtg ctcgaccccg
    13621 tcgagcacga aacgcgagtg cgtcccgaga ccatcgggct cggtggagag cacccgcagg
    13681 tcggcccccg gctcgtagcc gtaggtcacg acggccccac cgcatgagcg caccgcctgc
    13741 gcgaggcggc gcgacccggg gtcgtcgaag caggcgacga cgaggccgtc ggcgggcacc
    13801 gtggcgacga acgcggcgta cgcctcctcg acccgctcgg cggtgccgta gaagtcgagg
    13861 tggtccggct ggacgttggt cacgacggcg acgtccgggc ggtaggcgag gaaggagccg
    13921 tcgctctcgt ccgcctcgac gacgaacacc gagccatccc cgatcgcggc gttggtggcg
    13981 agctgggcga tctcggcccc gcaggcgtag gacgggtgcg cgcccgcggc gtcgagcgcg
    14041 acaacgagca tcgacgtcgt cgtcgtcttg ccgttcgccc ccgcgacggc gacgcgtcga
    14101 gagccggcca tcagggacgc cagggcctgc gagcggtgca gcacccgcag cccggcagcg
    14161 cgcgccgcgg caagttcgac gttgtcctcc cggatcgccg acgacacgac gacggtgtcg
    14221 accccggcga ggtgcccggc gtcgtggccg aggtgcacgc gggcgccgag ggtgcgcagg
    14281 gcctgcaggg ccggcccgtc ggcggcatcc gacccgctga cgctcacccc acgggcgagg
    14341 agcaggcggg ccaccgcgga catcccggca ccaccgatcg cgatgaggtg cacccggccg
    14401 aggtcggcga gcggtgtggc cggcgccgtg acgtcgaacc tcatgtcggg cagggtcatc
    14461 gggcgtgccc cgccgtcgtg gtggcccccg cagcagcggc catcacgagg tccgcgagca
    14521 gctcgtcgcc gccgcgctcc cccaccgagg ccgaggccga ggccatgaca cccagtcgcg
    14581 ggccatcgag cgcgagcggg cgcacgacgt cgtcgaccca tgactcggtg accgcggagt
    14641 cctcgacgag caggccccca ccggcggcga tgacgttcgc cgcgttcagg cgctgttccc
    14701 cgttcccgat gggcaggggc acgtagatgg caggcaggcc gaccgctgtc agctcacaga
    14761 cggtgttcgc gccggagcgg gcgaccacga ggtcggccgc ggcgtaggcg aagtccatgc
    14821 ggtcgcagta cgcgagcacg acgtagggcg caccggtgac gccctcgggc acgaagtcct
    14881 tgccggtgcc cgcgacgtgc agcacctgca cccctgcgcg ggacaggcgt gccgccgcgg
    14941 cggcgaaggc gtcgttgaga cgctgcgccc cgagcgaccc accggtgacg agcacggtcg
    15001 gggcgtcgct cagaccgaag tgggccctgg cctgctcgcg cagggcaccc cggtccaggg
    15061 tcgcgatctc gcgccgcaac ggcatcccgg tgacggtggc cctcggcagc ggggtgtccg
    15121 ggaaggtcac gcccaccgcg gatgcgatcc gggcgccgag gcggtt
//
LOCUS       DAOSOC010000044        15136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_3956_length_15136_cov_8.373583, whole genome shotgun sequence.
ACCESSION   DAOSOC010000044 DAOSOC010000000
VERSION     DAOSOC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 15136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15136
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3956_length_15136_cov_8.373583"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PL014_05290"
     CDS             <1..517
                     /locus_tag="PL014_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HOT56538.1"
                     /translation="ESSAPGEGFLTDVVLAWEASAAPAVDAGASVALARTGIVLARQG
                     GAMERLLQLARFGLAGPLGSGKQYWPWITLDDEVGALLHLLDRDDLTGPVNLSAPEPA
                     RQRDIVAAIGRALNRPAVLPAPSPALRIVLGEFATEILGSQRIVGEVLATSGYDFIHP
                     DLEGAARWLVA"
     gene            complement(463..3030)
                     /gene="mgtA"
                     /locus_tag="PL014_05295"
     CDS             complement(463..3030)
                     /gene="mgtA"
                     /locus_tag="PL014_05295"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HOT56539.1"
                     /translation="MEPATPYWSLEIEAVLDAVGSSPAGLGDVEARTRALAQGHGAAA
                     PSRARTIGRELGRQFSQPVVVILLVATGLSLVLGDRVDALIITAIVLLSGLLGFWQEH
                     GAAVTVARLLDRVQVHVEVRRAGRVVSVRPDEVVPGDVVVLNAGDIVPCDCRVLTAEA
                     LQTDESALTGETYPRHKHPDPAPADAALAERHSALLQGSHVVSGRGEAVAVLTGSHTE
                     LGRMSSTLTDRPTRTGFEQGTTRLGLLLARVTLGLTAVILVVNVVLGRPFIDSVLFSI
                     ALAVGVTPQMLPAIVAVSLSTGARRMADASVIVRRLDAIEDLGSMDLLCTDKTGTLTR
                     GVLTLAATVGPDGGHSDLVAGRAALNAALQTGFTNPLDDAVLAGAAPDQRWRAVDEVP
                     FDFDRKMLSVLVDGPEGRLLVTKGAYASVLARCSTVVGPGGAPEALGPRRMLLDEQFR
                     GLSEQGARVLAVAERPLPGRAEVTVDDERDLTLLGLLAFEDPPKDGLAATLAELEALG
                     IRLCMLTGDNALVATHVARSILAAEPTVVTGSEVDALDDIALRALAPTVDAFAELTPT
                     QKERIIKAVRAGGSVVGYLGDGINDAGPLHLADVGISVDTAVDVAKSAAAMVLLDKDV
                     DVVVSGVRLGRQTFTNTLKYVYTTVSANFGNTASMAAASAFLPFLPLLPRQILLLNFL
                     SDLPSITLAGDRVDPEDVQRPRRWNLHQVRDFMVVFGLLSSAFDLLTFAVLLQVFDAG
                     ATLFRTGWFVGSTLTELAVLFVLRTRRPVWRSHPGHALVLTSLLVGGVTVSLPFVSAA
                     LPALGLARPPVGLVVTLVGITGCYIVAAELTKRAFFRAGGQATSQRAAPSRSGWMKS"
     gene            complement(3211..4779)
                     /locus_tag="PL014_05300"
     CDS             complement(3211..4779)
                     /locus_tag="PL014_05300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT56540.1"
                     /translation="MDDEGTQPPEVPGFEVGELLARGGTSEVWAGVSVPEGRRVAIKV
                     VQAGLGEVEAAAREAAVSAAAASAHIVEIHSVIDLADGRVALVMPHLRGGSLDALVRT
                     RGHLTAGEVVTALAPVASALGRLHGLGVVHGDVSPGNVLLDLDGRPVLGDLGLGHVVG
                     DISPGVWGTDGYVAPEVVMGGDPSPASDVYGVGALGWLCLTGSVPGAPGLRPALADVS
                     RAGPSAAGLVEALEAALSPDPADRPDAHELGWLLFQAATATPLDLVRGGDEVSAVTYR
                     LRAAAGEAPVLEATPRWRRVWARLAGRGAAPPTSIPVERRRGAGRHSEPATRAGVGVA
                     RVAVTAAGAAALAVALVVAATVLGRDSGSAEARAAPSTVQPVGGPAQVRPTFVPDPRV
                     DPVSPRERPQELLTALAEARAGAFREATPAMLHGAVAPGSTAAARDAAAVSEVARAGL
                     RYSGLRYDVVDARHVSGDARAAVVQARIDASAYSVVGRSGAQPQPAQEGRVVVVDLVF
                     TQAGWRISAIRPVS"
     gene            4859..5599
                     /locus_tag="PL014_05305"
     CDS             4859..5599
                     /locus_tag="PL014_05305"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HOT56541.1"
                     /translation="MTADAPTILATSGGFRYGHRTDVEFAALVHHAVDLSGTHGRRPR
                     VTYVGTASGDPRFMNYRVIEAGRVAGFDVTCLDLFPMPNLDDVPGHLLEADVVWVGGG
                     SVANLLAVWDVHDLRPAMRAAWEAGVVLGGVSAGSICWYLGGCTDSFGPDLRAVTNGL
                     GLLPYGSGVHYDSEESRRPTIHALVGDGTLPTTHCTDDGVGLVYRGTELVEAVSEVDG
                     KGAYIVTRDGEGGGIREERIEPRRLPSP"
     gene            complement(5663..6694)
                     /locus_tag="PL014_05310"
     CDS             complement(5663..6694)
                     /locus_tag="PL014_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT56542.1"
                     /translation="MMRVALAGFGSSGQTIHAPLLHEAGCDVVAVSTRDPERAAAARA
                     EFPGTSVVDDLTALLAVPGVDLVVLATPSGAHAAQVRTVIDAGIACLVDKPLAVDAVS
                     AADVVRHAEASAVPLTVFHNRRYDPEHATVARVVADGLIGTPFRYEMRWERWRPVPKD
                     RWRENAGPADGGGILLDLHSHLVDAAVQLFGPIASVFATVAAHTTLAEDDAFLVCRHE
                     SGVVSHLGATALSGAPGPRVRMLGTTGAYVMADFTGETHVWTAQADADPGQSGWVYRG
                     DEREPVARGTSTQADLYRAVAAALESPEPQAAMPVDPWDAVHTLAVIDAARESAAQER
                     VVSVRTPAR"
     gene            complement(6705..>7046)
                     /locus_tag="PL014_05315"
     CDS             complement(6705..>7046)
                     /locus_tag="PL014_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56543.1"
                     /translation="ASDTGATASGMPSTSPPAQPSPSPAPRRLTPEADGSHYSMATGS
                     TTALVVREPGADEPEVDGASVILIPVVNVTGSGVREWEVRAVEPGTSVITSTTPAYTI
                     TLTVQASSGTP"
     assembly_gap    7047..7146
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7221..10514)
                     /locus_tag="PL014_05320"
     CDS             complement(7221..10514)
                     /locus_tag="PL014_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HOT56544.1"
                     /translation="MASLEDVMRARRSLSLGLGAVLVLSLAAPVAAAAAAPDRKPGAS
                     SASPPGKGDLVRRNAFGYVESTSSPAGLTKPSKAPAEDIAVAYAKSAGSDFGLGSGAG
                     KTLVVTKAQRLTTGATAVHVGQRVEGLRVTDAVLTVVVSPDGRVMSASGRLAKGTTSR
                     GPSAGLTARQALDVSAAKQGAKPDRPLREADVRSTGKKTFPNVYGQGSASERPVTAEL
                     AWYPSADGQSLRLAWVTDIESSGQQWWESVVDADTGAVLERTSRYAHAGPEGTVWREQ
                     HPEAAGATRQVTAFTGLDGSWVNGTTTSGNNVNAYLDRNDDDANNEYQPQTPASGDPG
                     YQHFNYAFGDTWRSTADVDSLAALDADRDAAITQLFYYTNVMHDWLYGHGFDEASGNF
                     QIDNYGRGGSGNDPVLAEAQDGWDFGCVDDKGTNDTSDDDPIRCLNNANFGTPVDGQS
                     PRMQMYMWAPTRPYRDGDVDGDVIAHEYGHGVSSRLVGGGDLGYNDGDQRGALGEGWS
                     DVISYLKWGDAVIGEYVTGNASTGIRSVAYNASNLDFQDYDVNASSGHGPGQIWASAL
                     YDIRDQLPGGVEAMASLVLDGMKATTANPTFLQARDGLLAADGNANRCLIWSAFAGRG
                     LGTGSTGSLDTVPTFSDDIPDQCLPTADAGGPYVTPEGTDVSLDGTGSAAGSDPSAGA
                     IATYEWDLDNDGAYDDATGANPTFSTVGQDGVFPIGLRVTDTFGNTATATSQVTVTNV
                     APTVSITAITAIDEGGTVTVSGTVTDPGWLDPLTATISFDDGASPVALAGTLENVRPN
                     ATLTFSVDHQYGDDGAFTVQVCAADDDTTGNCDSEVATVGNIDPTAVIDTSGEQVYDG
                     VSAFILEAGENLTVPVGSVDPGSDDLTAKWAWGDGSSDTQTSLVNPPGTDPAKSPSNQ
                     PRDVDFSKTHAYVDACAYTLDVTVTDDDLGSAADSALVLITGNADVSKGHGWWLNQYR
                     TKAPNDFTPAELQCYLDIVGALSMVFDEKTDASTRAKATKVLNSPAKSPANVIFDQQL
                     LGAWLNFANGSVKLATPVDTDGNGSKDSTFGAAVFTAESVRINPASSSAQIKAQKDII
                     ERVALQSGN"
     gene            complement(10591..>12675)
                     /locus_tag="PL014_05325"
     CDS             complement(10591..>12675)
                     /locus_tag="PL014_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT56545.1"
                     /translation="QTNDNSTFLPENAESTLVSEAVGRFASSDSLPYLVVFTRADGAP
                     LAAPDLESVGRFAAGVPALALPELGDGVVLADFLVGDAPLVPIPSQDGAAALLSVPLD
                     GVTGTAASGETSALVEGADALRASIDETLVPAGLSANVGGPGGLIADFAEAFAGIDGI
                     LLAVALSVVLVILLIVYRSPILPFAVLLSAVFGLSAASLVVYQLAVNDVITLSGQSQG
                     ILFILVVGAATDYALLLVSRFKEALHDEASSWVALKRAWFGAVEPIVASAATVILGLL
                     CLLLAELRSTSGLGPVGALGIAGAMLASLTFLPAVLLLFGRKVFWPFVPKLDHVHAED
                     VVGTRGVWGRVADLVGKHPRRTWVLTLAALLAAAAFAPTFTASGSTIEETFLDDTDAI
                     VAQRVIGEHFPAGSATPVQVVVPQADAEAALAVVTADDGVESAFIGMVPPMPGAPAVP
                     PPVVDGTVLIQATTTAASDTADARDTVERLRSDLDEVSSQALVGGNAATNLDVLDASA
                     RDLKVLVPTILLVIFLVLSLLLRSIVAPLLLVAANLVSFAATIGIAALVFEHGFGFTG
                     GDPAIPLYGFVFLVALGIDYSIFLMTRVREEATVRGTRPGILVGLAVTGGVITSAGIV
                     LAATFSALAVLPLVFLVQIAFIVAFGVLLDTFVVRSLLVPAAAYDLGPPVWWPKFLRA
                     DLRKDEELSPIP"
     assembly_gap    12676..12775
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12776..13786
                     /gene="lipA"
                     /locus_tag="PL014_05330"
     CDS             <12776..13786
                     /gene="lipA"
                     /locus_tag="PL014_05330"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOT56546.1"
                     /translation="EGRRMLRVEARNAQTPIERKPEWIRTTAKMGPEYTAIHSLVKGE
                     GLHTVCQEAGCPNIFECWEDREATFLIGGDTCTRRCDFCDIATGRPQALDLDEPRRVA
                     ESVRTMGLRYSTVTGVARDDLPDGAAGLYAETIRQIHALNPTTGVEILPPDFGAQPEL
                     VGQVFDARPEVFAHNLETVPRIFKRIRPAFTYDKSLKVLSMAREASLVTKSNLILGMG
                     EEDHEIEEAIRDLHDAGCDILTITQYLRPSPKHHPIDRWVKPEEFVHWSALAEDIGFK
                     GVMAGPLVRSSYRAGRLWATAMHRWGRPIPEHLAHLEAASGDPARQEAASLVAAAART
                     VS"
     gene            13851..14576
                     /locus_tag="PL014_05335"
     CDS             13851..14576
                     /locus_tag="PL014_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HOT56547.1"
                     /translation="MSEQPEKKTGRIAQARQAYSAIKSLDPKLGWWMLFAALGTAALL
                     LAGGFLLGGWWRWYAVLVAIPSALLAAVIVMNRRGNTAMYKALDGQVGATGATLTGLG
                     RRGWYAAQEPVALEGARGTRPSDMVDAAMVFRALGRPGVVLLGEGPPARVNKLLKQEE
                     KKVARVAPGVPVHLWVVGDGEGQVPVKKVAGKLTRMRPVLTKAETAVVNKRLKSLGGM
                     RPPIPKGVDPTRARVDRKAMRGK"
     gene            complement(14718..>15136)
                     /locus_tag="PL014_05340"
     CDS             complement(14718..>15136)
                     /locus_tag="PL014_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase LigA"
                     /protein_id="HOT56548.1"
                     /translation="RGGFVKLSGIIIAVIGAIMFVGGASTWVLVQNQLSAENIVVAED
                     ASFLAGDNVNGPFSAYAQAQIINEHALDASGGKTYAELDREDPVRAVVMNGSFLRASL
                     FTSVLAFGVSAMAMGLGLVQVLGGVALHKLGRHEDL"
BASE COUNT         2161 a         5316 c         5408 g         2051 t
ORIGIN      
        1 cgagagcagc gcgccgggcg aggggttcct caccgatgtc gtcctcgcgt gggaggccag
       61 cgcggctccc gcggtggatg ccggggcgtc cgtcgcgctg gcccgcaccg gcatcgtcct
      121 ggcccgtcag ggcggcgcga tggaacggct gctgcagctt gcccggttcg ggctggccgg
      181 gccgctcggc tccgggaagc agtactggcc gtggatcacg ctggacgacg aggtcggcgc
      241 actgctgcac ctgctcgacc gcgatgacct caccggcccg gtcaacctgt cggcgcccga
      301 accggcgcgt cagcgcgaca tcgtcgcggc catcgggcgc gccctcaacc ggccggcagt
      361 cctgccggca ccgtcgcccg ccctgcgcat cgtgctcggc gagttcgcca ccgagatcct
      421 cgggagccag cgcatcgtcg gcgaggtgct ggccacgagc ggttacgact tcatccaccc
      481 cgacctcgag ggcgcggcgc gctggctggt cgcctgacct ccggcccgga agaaggcccg
      541 cttggtgagc tcggcggcca cgatgtagca cccggtgatg cccacgaggg tgacgaccag
      601 gccgaccggc gggcgggcca ggcccagtgc gggaagcgcc gcggagacga acggcagcga
      661 gacggtgacg ccgccgacga gcagcgaggt gagcaccagg gcatggcccg ggtggctgcg
      721 ccacacgggt cggcgggtgc gcagcacgaa cagcacggcc agctcggtga gggtcgaccc
      781 gacgaaccag ccggtgcgga acagcgtggc gcccgcgtcg aagacctgga gcagcacggc
      841 gaaggtcagc aggtcgaagg cgctgctgag cagcccgaac accaccatga agtcgcgcac
      901 ctggtgcagg ttccaccggc gggggcgctg cacgtcctcg gggtcgacgc ggtcgccggc
      961 gagggtgatg ctcggcaggt cggagaggaa gttgagcagc aggatctgcc ggggcagcag
     1021 cgggaggaac ggcaggaagg ccgaggccgc cgccatgctc gcggtgttgc cgaagttcgc
     1081 gctgaccgtc gtgtacacgt acttcagcgt gttggtgaag gtctgccggc cgagccggac
     1141 gccgctcacg acgacgtcca cgtccttgtc gagcaacacc atggccgccg cgctcttcgc
     1201 cacgtcgacc gccgtgtcga ccgagatgcc cacgtcggcg aggtgcagcg ggccggcgtc
     1261 gttgatgccg tcgccgaggt agccgaccac cgatcccccg gcgcgcaccg ccttgatgat
     1321 tcgctccttc tgcgtcggcg tgagctcggc gaacgcgtcg acggtcggcg cgagcgcccg
     1381 cagtgcgatg tcgtccaggg catcgacctc gctgccggtc acgacggtgg gttcggccgc
     1441 gaggatgctg cgcgcgacgt gggtggcaac cagggcgttg tcccccgtca gcatgcacag
     1501 ccggatgccg agcgcctcaa gctctgccag ggtcgccgcg agcccgtcct tgggtgggtc
     1561 ctcgaaggcc agcaggccca ggagggtgag gtcgcgctcg tcgtcgacgg tgacctcggc
     1621 gcgaccgggc agcgggcgct cggccaccgc cagcacgcgg gcaccctgct cgctcagccc
     1681 acggaactgt tcgtcgagca gcatccgtcg tggcccgagc gcctcgggtg cgcctccggg
     1741 gccgaccacg gtggagcacc tcgccagcac cgacgcgtag gcacccttgg tcaccaggag
     1801 gcgcccctcg gggccgtcga cgagcaccga cagcatcttg cggtcgaagt cgaagggcac
     1861 ctcgtcgacg gcgcgccacc gctggtcggg tgccgcgccg gccaggaccg cgtcgtccag
     1921 cgggttcgtg aagccggtct gcagggccgc gttgagcgcg gcccgccccg ccacgaggtc
     1981 gctgtggccg ccgtcggggc ccacggtcgc cgcgagggtc agcacaccgc gggtgagggt
     2041 tccggtcttg tcggtgcaca gcaggtccat gctgccgagg tcctcgatgg cgtccaggcg
     2101 gcgcacgatc accgaggcgt ccgccatccg gcgcgccccg gtcgacaggg agaccgcgac
     2161 gatggccggc agcatctgtg gagtcacgcc gaccgcgagg gcgatcgaga agagcacgga
     2221 gtcgatgaac gggcgcccga gcaccacgtt gaccacgagg atgaccgcgg tgagcccgag
     2281 cgtcacccgg gcaagcagca ggcccaggcg cgtggtgccc tgctcgaagc cggtgcgcgt
     2341 cgggcggtcg gtgagggtgc tcgacatccg cccgagctcg gtgtgcgagc ccgtgaggac
     2401 cgccacggcc tcaccgcgac cgctcacgac gtggctgccc tgcaggaggg cgctgtgccg
     2461 ctccgcgagc gcggcgtcgg cgggcgcggg gtcggggtgc ttgtggcgcg ggtaggtctc
     2521 gccggtcagc gcggactcgt ccgtctgcag ggcctcggcg gtgagcaccc ggcagtcgca
     2581 cggcacgatg tcgccggcgt tcagcaccac gacgtcgccc ggcaccacct cgtcggggcg
     2641 caccgacacc acccgccctg ctcgccggac ctcgacgtgc acctgcaccc ggtcgagcag
     2701 ccgggcgacg gtcacggcgg ccccgtgctc ctgccagaac cccaggaggc cgctgagcag
     2761 cacgatggcg gtgatgatca gcgcgtcgac ccggtcgccg agcacgagcg agagcccggt
     2821 ggccacgagg aggatgacca ccaccggttg ggagaactgc cggcccagtt cacgcccgat
     2881 cgtcctggca cggctcggcg cagcggcgcc gtgcccctga gccagggcgc gggtgcgggc
     2941 ctcgacgtca ccgagacccg cggggctgct ccccaccgcg tcgagcaccg cctcgatctc
     3001 gagcgaccag tagggcgtcg ccggctccac gcccggagcc gggacgaagg cacctcgacg
     3061 cgccgagtgc ctcaccgtgg gcgaccaccc tccatgaccc ccagtgtcct cccgccgagc
     3121 cgcgcccata cgggcctgcg tcagatgtca cggcacggca ccgacgtcga cgatgcgccc
     3181 ccggcctcga gggcgcagcg cgctggctcg tcagctgacc gggcggatgg cgctgatccg
     3241 ccaccctgcc tgcgtgaaga ccaggtcgac gaccacgaca cggccctcct gcgcgggttg
     3301 cggctgcgcc ccggagcgac cgacgaccga gtaggcactg gcgtcgatgc gagcctgcac
     3361 gacggcggct cgtgcatccc ccgagacgtg ccgggcatcg acgacgtcgt accgcaggcc
     3421 cgagtagcgc aggccggccc gcgcgacctc cgacaccgca gcagcgtccc gcgcggccgc
     3481 ggtcgatccc ggcgccacgg ccccgtgcag catcgccggg gtcgcctccc gaaaggcccc
     3541 ggctctggcc tcggcgagcg cggtgagcag ttcctgcggg cgctcccgcg gggacaccgg
     3601 gtctaccctg gggtcaggga caaacgtcgg cctcacctga gctgggccgc ccaccggctg
     3661 cacggtcgac ggtgccgctc gcgcctcggc tgatccgctg tccctgccga gcacggtggc
     3721 agccacgacg agcgccacgg cgagggccgc ggcaccggcg gcggtcacgg cgacccgggc
     3781 gacacccacc cccgcccggg tcgccggctc gctgtgccga ccggccccac ggcgccgctc
     3841 gaccgggatg gacgtcggcg gcgcggcacc gcgtcccgcg aggcgtgccc acacgcgccg
     3901 ccaccgcggg gtcgcctcga gcaccggggc ctcgccggcg gcagctcgca gccgataggt
     3961 cacggcgctg acctcgtcac ccccgcggac gaggtcgagc ggtgtcgcgg tggccgcctg
     4021 gaacagcagc cagccgagct cgtgagcatc cgggcggtcg gccggatccg gggacagcgc
     4081 cgcctcgagg gcctccacca gccccgctgc agacggcccc gcgcggctca cgtcggcgag
     4141 tgccgggcgc aaccctggtg caccgggcac gctgccggtg aggcagagcc agcccagggc
     4201 gccgaccccg tagacgtccg atgccggtga cgggtcaccg cccatgacga cctcgggggc
     4261 gacgtacccg tcggtgcccc acactcccgg ggagatgtca cccaccacgt ggcccagacc
     4321 gaggtcaccg agcaccggcc ggccgtcgag gtcgagcagc acgttgcccg gggagacgtc
     4381 accgtggacg acaccgagac cgtgcaggcg ccccagggct gaagccaccg gggccagcgc
     4441 cgtcacgacc tcccccgccg tcaggtgccc gcgcgttcgc acgagcgcgt cgagggaccc
     4501 accgcgcagg tgcggcatga ccagcgccac ccggccgtcc gccaggtcga tgaccgagtg
     4561 gatctcgacg atgtgcgctg acgccgcggc ggccgacacc gccgcctcgc gcgccgccgc
     4621 ctccacctcc ccgaggccgg cctgcaccac cttgatcgcg acccgccgac cttcgggcac
     4681 cgacacgccc gcccagacct cgctcgtgcc gccccgcgcc aggagttccc ccacctcgaa
     4741 ccccggcacc tccggcggct gcgtcccctc gtcgtccatg cccccactgt gccgcgttcc
     4801 acgaaagcgc ccggagagtt atccacaggc agcccagcgg gcggctaccg tcgtgcccat
     4861 gactgccgac gccccgacca tcctcgccac ctcgggtggc ttccgctacg gacaccgcac
     4921 ggatgtcgag ttcgccgcgc tcgtgcacca cgcggtcgac ctctcaggca cccacgggcg
     4981 ccgaccacgc gtcacctacg tcggcacggc atccggcgac ccccgcttca tgaactaccg
     5041 cgtcatcgag gccgggcgcg tggccgggtt cgacgtcacg tgcctggacc tgttccccat
     5101 gcccaacctc gacgacgtcc ccgggcacct gctcgaggct gatgtcgtgt gggtcggtgg
     5161 cggctcggtc gcgaacctgc tcgcggtgtg ggacgtccat gacctgcgac cggcgatgcg
     5221 cgccgcgtgg gaggcgggcg tcgtgctcgg tggcgtgtcc gcggggtcga tctgctggta
     5281 cctcggcggc tgcaccgact ccttcggccc cgacctgcgc gcagtgacca acggtctcgg
     5341 cctgctgccc tacggcagcg gcgtgcacta cgactcggag gagagccgcc gcccgacgat
     5401 ccacgccctc gtgggtgacg gcacgctccc cacgacccac tgcaccgacg acggcgtggg
     5461 cctggtctac cgcggcaccg agctcgtcga ggcggtcagc gaggtcgacg gcaagggtgc
     5521 gtacatcgtg acccgcgacg gtgagggcgg cggcatccgg gaggaacgca tcgaaccgcg
     5581 acgcctgccc tccccctgac cacgacttct tgcactccgg ccacctcccg caggtggctg
     5641 gagtgcaatg agtcggatgc cgtcagcgtg cgggggtgcg cacgctcacc acgcgctcct
     5701 gcgccgccga ctcgcgcgcc gcgtcgatga cggccagcgt gtgcacggca tcccacgggt
     5761 ccacgggcat tgcggcctgg ggctccgggg actccagcgc tgccgccacc gcgcggtaga
     5821 ggtcggcctg ggtgctcgtg ccgcgggcca ccggctcacg ctcgtcgccg cggtacaccc
     5881 agccgctctg gccggggtcg gcgtcggcct gagcagtcca cacgtgggtc tcccccgtga
     5941 agtccgccat gacgtaggcc ccggtcgtgc cgagcatccg cacccgcggc cccggcgcac
     6001 cggacagcgc ggtcgccccc aggtgcgaga ccacccccga ctcgtggcgg cagacgagga
     6061 acgcgtcgtc ctcggcgagg gtcgtgtgcg cggccacggt ggcgaacacc gaggcgatcg
     6121 gcccgaacag ctgcacggca gcgtcgacga ggtggctgtg caggtcgagc aggatgccgc
     6181 cgccgtcggc ggggccggcg ttctcgcgcc accggtcctt gggcaccggc cgccagcgct
     6241 cccaccgcat ctcgtagcgg aacggcgtgc cgatcagccc gtcggcgacc accctcgcga
     6301 cggtggcgtg ctcggggtcg tagcgccggt tgtggaagac ggtgaggggc actgcgctgg
     6361 cttcggcatg gcgcacgacg tcggccgccg acacggcatc caccgccaag ggtttgtcga
     6421 ccaggcaggc gatccccgcg tcgatgacgg tgcgcacctg cgcggcgtgg gcgccgctgg
     6481 gggtggccag caccaccagg tcgacgcccg gcaccgcgag cagcgcggtc aggtcgtcga
     6541 cgacgctcgt tccggggaac tcggccctgg cggccgcagc gcgttcagga tcgcgggtgg
     6601 acacggcgac gacgtcacac ccggcctcgt ggagcagcgg ggcgtggatg gtctgaccgg
     6661 aggaaccgaa cccggcgagg gcgacgcgca tcatggcgcc caatctaggg cgtgccggac
     6721 gacgcctgaa ccgtcagcgt gatcgtgtac gccggggtcg tgctcgtgat gacgctggtg
     6781 ccgggctcga cggcgcgaac ctcccactcg cgtacgccgg agccggtgac gttgacgacc
     6841 gggatgagga tcacggacgc cccgtcgacc tcgggctcgt cggcacccgg ctcccgcacg
     6901 accagcgcag tggtcgagcc cgtcgccatc gagtagtggc tgccgtcggc ctcgggcgtg
     6961 agccggcgag gggccggcga cggcgacggt tgcgcgggtg gtgacgtgga cggcatcccg
     7021 gatgccgtgg cgccggtgtc ggatgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnngacg agcagcacca ccgcgagcgg cccggtgacg gcccggacgg ccgtcaccgg
     7201 gacctgtgca cccatcgcga tcagttgccg ctctggagcg cgacccgctc gatgatgtcc
     7261 ttctgcgcct tgatctgggc actggaggac gccgggttga tgcgcaccga ctcggccgtg
     7321 aacaccgctg cgccgaaggt gctgtccttg ctgccgttgc cgtcggtgtc gaccggtgtc
     7381 gcgagcttga ccgacccgtt ggcgaagttc agccaggccc cgagcagctg ctggtcgaag
     7441 atgacgttcg ccggggactt ggccggggag ttcagcacct tggtggcctt ggcccgcgtc
     7501 gaggcgtcgg tcttctcgtc gaacaccatg ctgagcgctc cgacgatgtc gaggtagcac
     7561 tgcagctccg ccggggtgaa gtcgttcggg gccttggtgc ggtactggtt cagccaccag
     7621 ccgtgcccct tggagacgtc ggcgttgccg gtgatgagca cgagcgcgct gtcagccgcc
     7681 gagccgaggt cgtcgtcggt caccgtcacg tccagggtgt aggcgcacgc gtcgacgtag
     7741 gcgtgggtct tgctgaagtc gacgtcgcgc ggctggttgc tcgggctctt cgccgggtcg
     7801 gtgccgggcg ggttgaccaa cgaggtctgc gtgtcgctcg acccgtcgcc ccaggcccac
     7861 ttggcggtga ggtcatcgct gcccgggtcc acggatccga cgggcacggt caggttctca
     7921 ccggcctcga ggatgaacgc cgagacgccg tcgtagacct gctcgcccga ggtgtcgatg
     7981 accgccgtcg ggtcgatgtt gccgacggtc gcgacctcgc tgtcgcagtt gccggtggtg
     8041 tcgtcgtcgg cggcgcacac ctgcacggtg aacgcgccgt cgtcgccgta ctggtggtcg
     8101 accgagaacg tcagcgtcgc gttcgggcgc acgttctcga gggtgccggc gagggcgacc
     8161 ggggacgcgc cgtcgtcgaa ggagatcgtc gccgtgagcg ggtcgagcca gcccgggtcg
     8221 gtgacggttc ccgacacggt gaccgtgccg ccctcgtcga tcgccgtgat cgcggtgatg
     8281 ctcacggtcg gggcgacgtt cgtcacggtc acctgcgacg tcgcggtcgc ggtgttgccg
     8341 aaggtgtcgg tcacccgcag cccgatcggg aagacgccgt cctgcccgac cgtggagaac
     8401 gtcgggttcg caccggtcgc gtcgtcgtag gcgccgtcgt tgtcgaggtc ccactcgtag
     8461 gtggcgatgg cgcccgcaga cgggtcgctt ccggcggccg agccggtgcc gtcgagcgac
     8521 acgtcggtgc cctcaggcgt cacgtacgga ccgcccgcgt cggcggtggg aaggcactga
     8581 tccgggatgt cgtcactgaa ggtcggcacg gtgtccaggc ttccggtgga ccccgtcccg
     8641 agacctcggc cggcgaacgc cgaccagatg aggcaccggt tggcgttgcc atcggcggcg
     8701 agcagcccgt cgcgcgcctg caggaaggtc gggttggccg tggtggcctt catgccgtcg
     8761 agcacgaggg aggccatagc ctcgacaccg ccgggcagct ggtcgcggat gtcgtagagc
     8821 gcgctcgccc agatctggcc cggcccgtga ccgctgctgg cgttgacgtc gtagtcctgg
     8881 aagtcgaggt tggaggcgtt gtaggccacc gaccggatgc cggtgctcgc gttgcccgtc
     8941 acgtactcac cgatgacggc gtcgccccac ttcaggtagg agatgacgtc gctccagccc
     9001 tcgccgagcg cgccgcgctg gtcgccgtcg ttgtagccga ggtcgccacc accgacgagt
     9061 cggctggaca cgccgtgccc gtactcgtgg gcgatgacgt caccgtcgac gtcaccgtcg
     9121 cggtagggcc gggtcggcgc ccacatgtac atctgcatac gcgggctctg cccgtcgacc
     9181 ggggtgccga agttcgcgtt gttcaggcac cggatgggat cgtcgtcact ggtgtcgttg
     9241 gtgcccttgt cgtcgacgca gccgaagtcc cagccgtcct gggcctcggc gagcacgggg
     9301 tcgttgcccg aaccgccccg gccgtagttg tcgatctgga agttgccgct ggcctcgtcg
     9361 aagccgtggc cgtagagcca gtcgtgcatg acgttggtgt agtagaagag ctgggtgatc
     9421 gcggcgtccc ggtcggcatc cagggccgcg agggagtcga cgtcggcggt gctgcgccag
     9481 gtgtcgccga aggcgtagtt gaagtgctgg tacccagggt ctccgctcgc gggggtctgc
     9541 ggctggtact cgttgttcgc gtcgtcgtcg ttgcggtcga ggtaggcgtt gacgttgttg
     9601 ccgctcgtcg tcgtcccgtt gacccacgat ccgtcgaggc cggtgaacgc ggtgacctgc
     9661 cgggttgccc ccgcggcctc cgggtgctgt tcgcgccaca ccgtgccttc tggcccggcg
     9721 tgcgcgtacc ggctcgtgcg ctccagcacc gctccggtgt cggcgtcgac gacgctctcc
     9781 caccactgct gcccggagga ctcgatgtcg gtcacccagg ccaggcgcaa cgactggccg
     9841 tccgcgctgg ggtaccaggc gagctcggcc gtcacgggcc gctcgctggc cgagccctgc
     9901 ccgtagacgt tcgggaaggt cttcttcccg gtgctgcgca cgtcggcctc acgcagcggc
     9961 cgatcgggct tcgcgccctg cttggcggcc gagacgtcaa gggcctggcg ggcggtcaga
    10021 cctgccgacg ggcccctgga cgtggtcccc ttggccagcc gcccggatgc cgacatcacc
    10081 cgaccgtcgg gtgacacgac caccgtgagc acggcatccg tcacccgaag tccctcgacg
    10141 cgctgtccca cgtgcacggc ggtcgcgccg gtcgtcaggc gctgcgcctt ggtgaccacg
    10201 agtgtcttcc ccgcccccga gcccagaccg aagtcgctcc ccgccgactt ggcgtaggcc
    10261 acggcgatgt cctcggcggg tgccttcgag ggcttggtca gccccgcggg tgacgaggtc
    10321 gactccacgt acccgaaggc gttgcggcga acgaggtcgc ccttacccgg tggtgaggcg
    10381 ctcgacgctc ctggtttgcg atccggggct gcagccgccg cggcgacggg cgccgccagg
    10441 ctgagcacga gcacggcacc gagaccgagg gagagagatc gacgagcgcg catgacgtcc
    10501 tccagtgagg ccacctcgcc ccaacccggc cctgcgtcgg gctgtgagct acctcacgcc
    10561 ccgtcacgat cctctcgatc accaccgctg tcagggaatc ggcgaaagtt cctcgtcctt
    10621 cctgagatcg gctctcagga acttcggcca ccagaccggc gggccgaggt cgtaggccgc
    10681 ggcgggcacc agcagggaac ggacgacgaa ggtgtcgagc agcacgccga aggcgacgat
    10741 gaacgcgatc tgcacgagga agacgagcgg cagcacggcc agggccgaga acgtcgccgc
    10801 cagcacgatg ccagcgctcg tgatcacgcc tcccgtcacg gcgaggccga cgaggatgcc
    10861 gggtcgcgtc cccctgaccg tggcctcctc acggacgcgg gtcatgagga agatcgagta
    10921 gtcgatgccg agcgccacga ggaacacgaa cccgtagagc gggatcgcgg ggtctccccc
    10981 cgtgaagccg aagccgtgct cgaagaccag ggcggcgata ccgatggtcg ccgcgaagga
    11041 gacgaggttg gccgcgacga gcagcagcgg cgcgacgatg gagcgcagca gcagggacag
    11101 gacgaggaag atgacgagga ggatcgtcgg cacgaggacc ttgaggtcac gggcgctcgc
    11161 gtcgaggacg tcgaggttcg tggcggcgtt gccaccgacg agcgcctggc tcgacacctc
    11221 gtcgaggtcg gagcgcagtc gctccacggt gtcgcgtgcg tccgcggtgt ccgaggccgc
    11281 cgtggtcgtc gcctggatga ggacggtccc gtcgacgacc gggggcggaa cggcgggtgc
    11341 ccccggcatc ggcgggacca tcccgatgaa cgcgctctcg accccgtcat cggcggtgac
    11401 caccgcgagc gcggcctcgg catcggcctg cggcacgacg acctgcaccg gggtggccga
    11461 gcctgccggg aagtgttcgc cgatgacccg ctgcgcgacg atcgcgtcgg tgtcgtcgag
    11521 gaaggtctcc tcgatggttg acccgctcgc cgtgaaagtc ggggcaaagg ccgccgccgc
    11581 gagcagcgcg gccagcgtga gcacccacgt gcgccgcggg tgcttcccga ccagatcggc
    11641 cacccggccc cagacccccc gggtgccgac gacgtcctcg gcgtgcacgt ggtcgagctt
    11701 gggcacgaac ggccagaaga ccttgcggcc gaagagcagc agcacggcgg gcaggaaggt
    11761 caatgaggcg agcatggcgc cggcgatccc cagcgcaccg accggcccga gccccgacgt
    11821 gctgcgcagc tcggcgagca ggaggcagag cagaccgagg atcaccgtgg cggccgaggc
    11881 gacgatcggt tcgaccgctc cgaaccaggc ccgcttgagg gcgacccagg aactggcctc
    11941 gtcgtgcagg gcctccttga agcggctgac gaggagcagc gcgtagtcgg tcgcggcccc
    12001 cacgacgagg atgaacagga tgccctgcga ctgccccgac agcgtgatga cgtcgttgac
    12061 cgcgagctgg tagacgacca acgacgcggc gctcaacccg aacacggcgc tcagcagcac
    12121 ggcgaacggc aggatcgggc tgcggtagac gatgagcagg atgacgagca ccacgctgag
    12181 ggccacggcg agcaggatgc cgtcgatgcc ggcgaacgct tcggcgaagt cggcgatgag
    12241 gccacccggc cctccgacgt tggccgacaa tccggcgggg accagggtct catcgatgct
    12301 cgcccgcagg gcatccgccc cctcgaccag ggctgacgtc tcaccgctgg ccgccgtgcc
    12361 ggtgaccccg tcgagcggca cgctgagcag ggcggcggcg ccgtcctgcg aggggatcgg
    12421 gacgaggggg gcgtcgccga cgaggaagtc agcgagcacc acgccgtcac cgagttcggg
    12481 cagggccagg gctggcacac ccgcggcgaa gcggcccacg gactccaggt cgggggcggc
    12541 gagcggcgca ccgtcggccc gggtgaagac gacgaggtag ggcagcgagt cactgctcgc
    12601 gaagcggccc actgcctcgc tgaccagtgt ggactcggcg ttctcgggca ggaacgtcga
    12661 gttgtcgttg gtctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaggg
    12781 acgccgcatg ctgcgggtgg aggcgcgcaa cgcgcagacc ccgatcgagc gcaagccgga
    12841 atggatccgc acgacggcca agatggggcc cgagtacacg gccatccact cgctggtcaa
    12901 gggtgagggg ctgcacaccg tctgccagga agccggctgt ccgaacatct tcgagtgctg
    12961 ggaggaccgt gaggccacgt tcctcatcgg tggggacacc tgcacccggc ggtgcgactt
    13021 ctgcgacatc gccaccggcc gcccccaggc cctggacctc gacgaaccgc gccgcgtcgc
    13081 ggagtcggtg cgcacgatgg gcctgcgcta ctcgaccgtg accggtgtcg ctcgcgacga
    13141 cctgcccgac ggcgccgccg gcctctacgc cgagacgatc cgccagatcc acgcgctgaa
    13201 ccccaccacc ggggtcgaga tcctgccccc cgacttcggg gcccagcccg agctcgtcgg
    13261 tcaggtcttc gacgcgcggc ccgaggtgtt cgcgcacaac ctcgagacgg tgccgcgcat
    13321 cttcaagcgg atccgcccgg cgttcacgta cgacaagtcc ctcaaggtgc tgtcgatggc
    13381 gcgggaggcc tcgctggtca ccaagtccaa cctcatcctc gggatgggcg aggaggacca
    13441 cgagatcgag gaggccatcc gcgacctgca cgacgccggc tgcgacatcc tcacgatcac
    13501 ccagtacctg cgcccctccc ccaagcacca cccgatcgac cgctgggtca agcccgagga
    13561 gttcgtgcac tggtccgcac tggccgagga catcggcttc aagggcgtca tggcgggccc
    13621 gctcgtgcgc tcgtcctacc gcgccggccg gttgtgggcc acggccatgc accgctgggg
    13681 ccgtccgatc cccgagcacc tggcgcacct cgaggcggca tcgggcgacc cggcgcgtca
    13741 ggaggcggca tcgttggtcg cggcggcggc gcgcaccgta tcctgatctg cgacgcccgc
    13801 ggcatcccgc ggattccgcc cccctgactt catccaggag cacgactccc gtgtccgaac
    13861 agcccgagaa gaagaccggc cgcatcgccc aggcccggca ggcgtacagc gcgatcaaga
    13921 gcctggaccc caagctgggc tggtggatgt tgttcgccgc cctcggcacg gcggcgctgt
    13981 tgctcgctgg cggcttcctc ctcggtggtt ggtggcgctg gtacgccgtg ctcgtggcga
    14041 tcccgtctgc gctgctcgca gcagtgatcg tgatgaaccg acgcggcaac acggcgatgt
    14101 acaaggccct cgatggtcag gtcggcgcca ccggggccac gctcaccggg ctcggccgcc
    14161 gcgggtggta cgccgcccag gagcccgtcg ccctcgaggg cgcgcgcggc acccgaccga
    14221 gcgacatggt ggatgccgcg atggtcttcc gcgcgctggg acgccccggc gtcgtgctcc
    14281 tcggtgaggg cccgccggcc cgggtcaaca agctgctcaa gcaggaggag aagaaggtcg
    14341 cccgtgtggc tcccggcgtt ccggtgcacc tgtgggtcgt cggtgacggc gagggccagg
    14401 tgccggtgaa gaaggtggcc ggcaagctga cccggatgcg cccggtattg accaaggccg
    14461 agacggccgt ggtcaacaag cggctgaagt cgctcggcgg catgcgcccc ccgatcccca
    14521 agggtgtcga ccccacgcgg gcccgcgtcg accgcaaggc catgcgcggt aagtaactcg
    14581 ccacaccctc gtcccgcgac cccacggcat ccggatgccg cctcgaccgg gtggcagagg
    14641 acgaccgggt ggcagaggac agtgaacggc cccggctctg gtgagccggg gccgttctgg
    14701 tgcgtgtcgc tgaaggatca gaggtcttcg tggcggccga gcttgtgcag ggccactcca
    14761 ccgaggacct ggaccagacc gagacccatc gccatcgcgc tgacaccgaa ggccaggacg
    14821 ctggtgaaga gcgaggcgcg aaggaacgag ccgttcatga ccacggcgcg caccgggtct
    14881 tcacggtcga gctcggcgta ggtcttgcca ccggaggcgt caagggcgtg ctcgttgatg
    14941 atctgggcct gggcgtaggc gctgaagggg ccgttgacgt tgtcgccggc gaggaacgag
    15001 gcgtcctcgg cgacgacgat gttctccgcg gagagctggt tctggacgag cacccaggtg
    15061 ctcgcgccgc cgacgaacat gatcgcgccg atgaccgcga tgatgatgcc ggagagcttg
    15121 acgaatccgc cacgct
//
LOCUS       DAOSOC010000045        14704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4112_length_14704_cov_9.389173, whole genome shotgun sequence.
ACCESSION   DAOSOC010000045 DAOSOC010000000
VERSION     DAOSOC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14704
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4112_length_14704_cov_9.389173"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PL014_05345"
     CDS             complement(<1..439)
                     /locus_tag="PL014_05345"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOT56549.1"
                     /translation="MRRSRVPGSTTDQRLLDSRGSADWVHTDPWRVMRIQSEFVTGFG
                     ALAELGPAVSVFGSARTKPHDPVYELGCRVGAALVDAGYAVITGGGPGAMEAANKGAL
                     EAGGTSVGLGIELPFEAGLNEFVDLGVNFRYFFARKTMFVKYAQ"
     gene            complement(535..1629)
                     /gene="dapE"
                     /locus_tag="PL014_05350"
     CDS             complement(535..1629)
                     /gene="dapE"
                     /locus_tag="PL014_05350"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HOT56550.1"
                     /translation="MPLTPLALDLADDVTSLTAAVCDIESVSLDERALADAVEAALRP
                     LPHLTVARIGNTVVARTDLGRAERVVLAGHIDTVPLTVEPANLPTRRVQGQGGEVLWG
                     RGTVDMKGGVAVMLRIAHEVREPSRDLTFVFYEAEEIDSVHNGLLLVQQQAPDLLADA
                     DFAVLLEPTDARIEGGCKGTLRAEVSTKGIAAHSARPWKGHNAIHDAAEVLARLAAYE
                     PVTRVVDELEFREALGAVLISGGTATNVIPDRCVVTVNYRYAPSLTEEDAEAHVREVF
                     SGFDVVVVDNAGGARPGLHLPAAKAFVEALGLTVEAKQGWTDVARFSALGVPAVNFGP
                     GDPNLAHMDDEQCPVEQYTACEQALLRWLA"
     gene            1685..2617
                     /gene="dapD"
                     /locus_tag="PL014_05355"
     CDS             1685..2617
                     /gene="dapD"
                     /locus_tag="PL014_05355"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOT56551.1"
                     /translation="MTRTAWGHALITLTDDATVLDAWFPAPALGEPGGAGELAAPAGL
                     EALAVPDAVRGVRRELRLVSVDLDAPPADAPDGWLRLHLLSHRLCAPNSINLDGLFGV
                     LTNVVWTSQGPCAVDGFELTRARLLARGPVQVFGIDKFPRMTDYVVPAGVRIADADRV
                     RLGAHLASGTTVMHEGFVNFNAGTLGTSMVEGRISQGVVVGDGSDIGGGASTMGTLSG
                     GGTERVSIGERCLLGAESGLGIALGDDCVVEAGLYVTAGTKVSLPDGSVTKASELSGQ
                     SNLLFLRNSVTGAVQARPRDGHGIVLNSALHAND"
     gene            2639..3529
                     /locus_tag="PL014_05360"
     CDS             2639..3529
                     /locus_tag="PL014_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56552.1"
                     /translation="MPRTAKGAIALAEHEPRSRARRRLGALVVLAMLGVVAVGGYAAV
                     GYLRMLWTPEQCEVTAAGSTFQWAPDQASNAAAITAIAIKRDLPPRAATIAIATAMQE
                     SKVRNITYGDRDSLGLFQQRPSQGWGTPEQILNPEYSTNKFYDALVKVESYTDMEITD
                     AAQEVQRSAAGKAYAQHEAQARATASVLSGQTHAGMVCLLEDPAAPGDARSVADLIEQ
                     DFGVTADVDGDTVTLSTDDEDLAWAVGSWGVARAVDTGAVRVVVADREWQRGMGDSAR
                     QWSTTDAPSAGAPAVTIHLT"
     gene            complement(3580..4785)
                     /locus_tag="PL014_05365"
     CDS             complement(3580..4785)
                     /locus_tag="PL014_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOT56553.1"
                     /translation="MEPLTVAVHAGREDLAALGAHVPPVDQSSTYPLPSVELGGEAYE
                     RMATGGRPQQDDSLVYQRLWNPNVDRFERALAQLEGAPAGVAFASGMAAITACLLAAV
                     AAGRPHVVAVRPLYGGTDHLLATGLLGTRVTWARTDEVDAAIRPDTGLVIVETPANPS
                     LDLVDIADVVARAGTVPVLVDNTFATPVLQQPLAHGAALSVHSATKFIGGHGDLLGGV
                     VATDEEWAVRLRTVRAITGGLLAPQSAYLLHRGLQTLPVRVLTQQENATVVARWLEGR
                     PEVERVLHPSLAQCDPMGLVGRQMSGPGSVLSFALRGGYAAAAAVADNCRLVTHAVSL
                     GGVDTLIQHPAALTHRPVAPEARPDAGLLRLSVGLESPQDVVADLARALAVAHEQPTV
                     VERELSLAT"
     gene            4875..5369
                     /locus_tag="PL014_05370"
     CDS             4875..5369
                     /locus_tag="PL014_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT56554.1"
                     /translation="MAPHPTTDHRIPRRPLDDVDRALVELLSTDGRMPNAELARRVGI
                     AESTCAGRVKALRDGGVVLAIRGEVDLAALGRPIEAMVALRFTGHARESMDSFRDRVA
                     SIPGVVAAYHVAGADDFLVHISGATANDLRDLVLDHLTSVEGVAHAQTSLIFERVRGR
                     RPLG"
     gene            complement(5391..6677)
                     /locus_tag="PL014_05375"
     CDS             complement(5391..6677)
                     /locus_tag="PL014_05375"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOT56555.1"
                     /translation="MTDGATLSAAGKQLDLSLVRATEGNGGYDISSLLKETGNVTLDG
                     GFTNTASCTSAITYIDGDAGILRYRGYPIEQLAKQSSFIETSYLLIYGELPTPSQLAE
                     FEGRISQHTMLHEDLKGFFQGFPRDAHPMPVLSSAVSALSTFYQDSLDPFDKEQVEIS
                     TIRLLAKLPTIAAYAYKKSIGQPFLYPDNNLSLVENFLRMTFGVPATDYEIDPDLVRA
                     VDLLLVLHADHEQNCSTSTVRLVGSSQANLFASVSAGINALFGPLHGGANQAVLEMLE
                     RIEKADFSTDEFMTKVKNKEDGVRLMGFGHRVYKNYDPRAAIIKDTAHEILAKTAGGD
                     HLLEIAMRLEEIALADEYFIERKLYPNVDFYTGLIYKSMGFPTRMFTVLFALGRLPGW
                     IAQWREMIEDPTTKIGRPRQVYIGEGERNYVPVGER"
     gene            complement(6772..7920)
                     /gene="dapC"
                     /locus_tag="PL014_05380"
     CDS             complement(6772..7920)
                     /gene="dapC"
                     /locus_tag="PL014_05380"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HOT56556.1"
                     /translation="MLSLPDFPWDSLAATKQRASSFSDPTIDGPAGSGLVDLSVGTPV
                     DPTPAVVRDALAAASDAHGYPLTWGTPDLREAVATWFAARRGVPDVDPGGVLPTIGSK
                     ELVAWLPLLMGIGPGDVVAFPRVAYPTYDVGARLAGATPVAVDALTALGPLTAATTPK
                     LLWLNTPGNPTGKVLGIEHLAKVVSWAREHGVVVASDECYAELDWRPRAAGSREPPTT
                     PSILDPRVTGGSHEGLLCVYSLSKQSNLAGYRGAFVAGDPVLVGQLLEVRKHAGMIVP
                     WPVQRAMLAALSDADHVVAQKQRYAARRAALLPAVESFGLRVDDSEAGLYLWASAGED
                     CRVSIERLADRGILAAPGDFYGPAGRQHVRIALTATDERIEAAVRRLG"
     gene            complement(7937..8263)
                     /locus_tag="PL014_05385"
     CDS             complement(7937..8263)
                     /locus_tag="PL014_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOT56557.1"
                     /translation="MTYVIAQPCVDLKDKACIEECPVDCIYEGERSLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDVPEQWAEYYKANVEFFDDLGSPGGAAKLGMIPKDHPIIAALP
                     PQEHDE"
     gene            8285..9316
                     /locus_tag="PL014_05390"
     CDS             8285..9316
                     /locus_tag="PL014_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT56558.1"
                     /translation="MDERVELPEREPRVGSRVVVRHTLPEADPLTGATLTDVVGDLVE
                     TDAATLVVRTRRGLVTVPRHLVTALKEIPPTPSRRGSPHRALSIEDLQRVMVGAWPAM
                     ESERLGDWVLRASRGFTQRANSVVTAGSPARPLPEALDAVERWYAARDLPANLTIAGP
                     VGFDPASDLVGAEALRRGYRGRVATVTLTAPTRLIARQPPRRADNEVAGVQEVEVGRD
                     LTDDWFAAYRSYRAVDDRAARVVLTGSPDQVFATTRDAGGRVVGIGRLGLASAWGGIA
                     AMWVEPTMRRRGVADAMLTALARAAEGAGVASLHLQSDSDNHVALAVYGQRGFDRHHE
                     YVNLVSTTA"
     gene            complement(9326..11056)
                     /locus_tag="PL014_05395"
     CDS             complement(9326..11056)
                     /locus_tag="PL014_05395"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOT56559.1"
                     /translation="MTDLFAQTSSADDHGSAQPSSRRRWPFLLAGALVALVAAYVAAA
                     VVLGDRVPRGTTVAGVDVGGKDVDGARDALAAGLADVGTEPVTLTSPVGEVAVQPSDL
                     GVSVDIDTTVDGLVGFSLAPAHVWRNLFGGGEENAVVTVDEAVFTTAVEGARSDLDSE
                     PVEGSISVAGGKVALKEPTTGTETDVAGTVAAVQRWWPTQSTIKVAARSLEPKVSAQE
                     LARVKTEFADVAVSGPVTVKAGEKSFTLTPKAFAPAIVLAADDSGTITPKADAKKLSA
                     IVSAAAKKAGAEVVAKDAVVTFSGSTPTVTPDVPGVGLDDASVQTEVWKAITTPTRTA
                     TVTTKAVAPKFTTAIAKATLPKEKISSFTTYYPAGQPRVTNIKVASRVLNGTYIPPGG
                     QFSMNAILGKRTPDKGYVKAGIISGGRAASAYGGGISQVSTTIFNAAFFSGMELDAWT
                     PHYYYISRYPEGREATISWPDLHNKFTNTTDGGVRMEVIATNSSITVNFWGTKKYDVT
                     ATKSDRFDIVQPRRFTDDSPDCLDQSPVPGFKVTVGRIIKEKGKVVKTEKFTTNYRPE
                     DDVTCTNPRP"
     gene            complement(11053..11550)
                     /locus_tag="PL014_05400"
     CDS             complement(11053..11550)
                     /locus_tag="PL014_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT56560.1"
                     /translation="MSHHDLDPAQFRLAMGRLASGVTVLTTTSDGHDHAMTADTLTSV
                     SLDPILVLVCVENETRWLAAVAQAGVFGVSVLAADQRALAQWFATPGRPLHGQLDRAP
                     HRRGPHTDMALLDGALMHLECRVTTSHAAGDHMIVVAEVLSITLPDDVGPALVHFRGS
                     YGSIA"
     gene            complement(11547..12425)
                     /gene="mshB"
                     /locus_tag="PL014_05405"
     CDS             complement(11547..12425)
                     /gene="mshB"
                     /locus_tag="PL014_05405"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HOT56561.1"
                     /translation="MKGLLDDVAIRGQRPRLLFVHAHPDDETLATGVALAHHVARGDD
                     VHVLTCTLGEEGEVIPAPLAHLEGAAGDPLAHHRRGELTAAMRSLGVVHHLLGASSGD
                     AAATYRDSGMAGSAAAEHPRAWVGADLEEAAAAIRAVIDEVRPDVVVTYDATGGYGHP
                     DHVRTHDATVRAVAGTAVRPVLYVTLTPQSWVLQDREWLAEHVPGDRGLIVPGPDDPF
                     ASSVVPDAVVTHAVLDPSVVAAQSQALRCHETQVVVGDGWYALSNHVVSRLAGREGYG
                     RLDPLTGQLPEQEESA"
     gene            complement(12422..14215)
                     /locus_tag="PL014_05410"
     CDS             complement(12422..14215)
                     /locus_tag="PL014_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT56562.1"
                     /translation="MPVAHRHAGLAAVVLGTLVVAALVVAGVFVLRDEPTVDPDRATA
                     TSAVGERVNRDGGTIRALALGPVLTWDPQRLASRDDIAFAGRVFARTLTAYAPNVDPD
                     AQDRLVGDLATDTGTASDDLKTWSFTLREGILWQDGTPVTCADVAYGISRSFASKDLP
                     GGSTDALAVLAIPRQANGTSTYAGPYATGPAAAAGQAAFDKAVSCVGQKLTFTLSTPL
                     GDFNELVSQPAFGPVKKSVDKGPEGVYEVFSAGPYVLEKPWVPGEGGTFVRNPHWARS
                     ADPVRRAHPDRIEYVEGMDIQAITQQVMADTDGGRTSVSLSSAPPAIQQNITAVQSLR
                     ERSVNPRTGVVDYLVPNTRSAVFKDPLVRRALAAATNREAYVTAIGGPTAADPARSLI
                     PRSLAAAHEGDPVGAGNRGDAATAKALLAQAKRTEPVPFTVAYRSSATSDKAMAALVA
                     GWRLAGFEPTTRPITDDYFTAISDPKIAGEVDVFWSNWAPAWASASTILPPLFDSAIN
                     ITASGPGRDYGYWADPKLNSRMAQIQQIDDRAEREAAWVVVDQSLLEQGAYIGLAERR
                     ALYVAGSDIRNLSANTIAGGVVEFADIAVGP"
     gene            complement(14297..>14704)
                     /locus_tag="PL014_05415"
     CDS             complement(14297..>14704)
                     /locus_tag="PL014_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOT56563.1"
                     /translation="YEPWVGEISTRISGSLVADRSGPATAYAMMNLQERGTLFIAPTT
                     EVYEGMIVGENSRVDDMDVNITKEKKLTNVRSAGADVLERLAPPRALSLEQSLEFCRE
                     DECVEVTPEAVRIRKVELDQTLRARAAARARKS"
BASE COUNT         2104 a         5003 c         5390 g         2207 t
ORIGIN      
        1 cctgcgcgta cttcacgaac atcgtcttgc gggcgaagaa gtagcggaag ttcaccccga
       61 ggtcgacgaa ctcgttgagc ccggcctcga acggcagctc gatgcccagc cccacggacg
      121 tgccaccggc ttcgagggca cccttgttgg cggcctccat cgcccccggc ccaccgccgg
      181 tgatgacggc gtagccggca tccacgaggg cggcgcccac gcggcatccg agctcgtaga
      241 ccgggtcgtg gggcttggtg cgggccgagc cgaacacgct gacggccgga ccgagctcgg
      301 ccagggcgcc gaacccggtg acgaactcgg actggatgcg catgacccgc cacgggtcgg
      361 tgtgcaccca gtcggcggag cctcgactgt cgagcagccg ttggtcggtg gtcgagccgg
      421 gcaccctgga gcggcgcatg accaccgagc cctggtagta gtcgcggtgg ggactggcct
      481 gtgtcggggt cgggtccacg gcgccactgt atgcggcgcc cttcaccact cgcgtcaggc
      541 gagccagcgc agcagggcct gctcgcacgc ggtgtactgc tcgaccgggc actgctcgtc
      601 gtccatgtgg gcgaggttgg ggtcgcccgg cccgaagttc acggccggca cgccgagggc
      661 ggagaagcgg gccacgtcgg tccagccctg cttggcctcg accgtgaggc ccagggcctc
      721 gacgaacgcc ttggccgcgg gaaggtgcag ccccgggcgg gcaccgccgg cgttgtcgac
      781 caccaccacg tcgaaaccgg agaagacctc gcgaacgtgc gcctcggcat cctcctcggt
      841 gagcgagggc gcgtagcggt agttcaccgt gaccacgcac cggtcgggga tgacgttggt
      901 cgccgtgccg ccggagatga gcacggcgcc gagggcttcg cggaactcga gctcgtcgac
      961 gacccgggtg accggctcgt aggccgcgag gcgcgcgagc acctcggcgg cgtcgtggat
     1021 ggcgttgtgg cccttccagg gacgtgccga gtgcgccgcg atgcccttgg tgctcacctc
     1081 ggcgcgcagc gtgcccttgc agccaccctc gatgcgggcg tcggtgggct cgagcagcac
     1141 cgcgaagtcg gcgtcggcga gcaggtcggg agcctgctgc tgcacgagga gcaggccgtt
     1201 gtggaccgag tcgatctcct cggcctcgta gaagacgaag gtgaggtccc ggcttggctc
     1261 gcgcacctcg tgggcgatcc gcagcatgac ggcgacgccg cccttcatgt cgaccgttcc
     1321 gcgaccccag agcacctcgc cgccctgacc ctgcacgcgt cgggtgggca ggttggccgg
     1381 ctcgacggtg aggggcacgg tgtcgatgtg accggcgagc acgacccgct cggcgcggcc
     1441 gaggtcggtg cgggccacga cggtgttgcc gatgcgcgcc acggtgaggt ggggcagcgg
     1501 gcgcagggcc gcctcgacgg catccgcgag ggcgcgctcg tccaggctca ccgactcgat
     1561 gtcgcagacg gcggcggtga gcgaggtgac gtcgtcggcc aggtcgagcg caaggggtgt
     1621 gagcggcatg gcgtcagcgt aatcaccgca cgggcgtgtc cgtcggccac cgtaggcttg
     1681 tcgcatgaca cgcaccgcct ggggtcacgc cctgatcacg ctcaccgacg acgcgacggt
     1741 gctcgacgcc tggttccccg ccccggccct gggtgagccc ggcggcgccg gcgagctcgc
     1801 ggccccggcc ggtctggagg ccctcgcggt gccggatgcc gtgcgtggcg ttcgtcggga
     1861 gctacggttg gtgagcgttg acctcgacgc gcccccggcc gacgccccgg acggctggct
     1921 gcgcctgcac ctgctctccc accggctgtg cgctccgaac agcatcaacc tcgacgggct
     1981 cttcggggtg ctcaccaacg tcgtgtggac ctcacagggc ccgtgcgccg tcgacggctt
     2041 cgagctgacc cgggcccgcc tgctggcgcg cggccccgtg caggtgttcg gcatcgacaa
     2101 gttcccccgg atgaccgact acgtcgtgcc cgccggggtg cggatcgcgg atgccgaccg
     2161 cgtgcgtctg ggggcccacc tcgcgagcgg caccaccgtc atgcacgagg gcttcgtcaa
     2221 cttcaacgcc ggcacgctcg ggacctcgat ggtcgagggt cggatcagcc agggagtcgt
     2281 cgtcggcgac gggtccgaca tcggtggcgg cgcgtcgacc atgggcacgt tgtccggcgg
     2341 cgggacggaa cgcgtgagca tcggcgagcg ttgcctgctc ggagccgagt ccgggctcgg
     2401 catcgcactc ggtgacgact gcgtggtcga ggccgggctc tacgtcaccg caggcacgaa
     2461 ggtctcgctc cccgacggga gcgtcaccaa ggccagcgag ctgtccggtc agagcaacct
     2521 gctcttcctg cgcaactcgg tgacgggcgc ggtgcaggcc cggccgcgtg acgggcacgg
     2581 catcgtgctg aactctgcgc tccacgccaa cgactgacac ctgaaggact cctccaccat
     2641 gccccgcacc gccaagggcg ccatcgccct tgccgaacac gagccccggt cgcgggcgcg
     2701 acgccgcctc ggcgccctcg tggtgctcgc gatgctcggg gtggtcgccg tcggggggta
     2761 cgccgccgtc ggctacctcc gcatgctgtg gacgcccgaa cagtgtgagg tgaccgcggc
     2821 cggttcgacg ttccagtggg ctccggacca ggcgagcaac gcggccgcca tcaccgccat
     2881 cgcgatcaag cgcgacctgc ccccgcgggc ggccaccatc gcgatcgcca ccgccatgca
     2941 ggagtccaag gtccgcaaca tcacctatgg cgaccgcgac tcgctcggcc tgttccaaca
     3001 gcggccctca cagggctggg gtacgccaga gcagatcctc aacccggagt actcgacgaa
     3061 caagttctac gacgcgttgg tgaaggtcga gagctacacg gacatggaga tcaccgacgc
     3121 cgcccaggag gtgcagcgct cggccgcggg caaggcctac gcccagcacg aggcgcaggc
     3181 gcgggccacg gcatccgtgc tgtccggcca gacccatgcc gggatggtgt gtctgctcga
     3241 ggacccggca gcaccgggcg acgcccgctc ggtcgcggat ctcatcgagc aggacttcgg
     3301 tgtcacggcc gacgtcgacg gtgacacggt gacgctgagc accgacgacg aggacctcgc
     3361 gtgggcggtc ggctcgtggg gggtggcccg ggccgtcgac accggggcgg tgcgcgtcgt
     3421 cgtcgcggac cgggagtggc aacgcggcat gggcgactcg gcccgccagt ggagcaccac
     3481 cgatgcgccg tcggctggtg ctccggcggt cacgatccac ctcacctgat ccgtcgttcg
     3541 ggcgctcgag ggctcgagca ctcgagcgcc acggtcggct caggtggcga ggctgagctc
     3601 tcgctccacg acggtgggct gttcgtgcgc caccgcgagc gcgcgggcga ggtccgcgac
     3661 gacgtcctgc gggctctcga ggccgacgga gagtcgcagg agccccgcgt cgggccgggc
     3721 ctcgggtgcg accggtcggt gggtcagggc ggccggatgc tggatgaggg tgtcgacacc
     3781 gccgagtgag accgcgtggg tgaccaggcg acagttgtcg gcaacggcgg ccgccgcggc
     3841 gtacccaccg cgcagagcga acgacaggac ggagccgggg ccgctcatct gtcggcccac
     3901 gagccccatc gggtcgcact gcgccaggct ggggtggagc acgcgctcga cctcgggtcg
     3961 gccctccagc catcgtgcga cgacagtcgc gttctcctgc tgggtgagca ctcgcaccgg
     4021 cagcgtctgc agcccccggt gcaggaggta ggccgactgg ggtgccagga ggccgccggt
     4081 gatcgcccgg accgtgcgca gcctcacggc ccactcctca tcggttgcca ccacgccacc
     4141 gagcaggtcc ccgtgaccac cgatgaactt ggtggccgag tgcacgctga gtgcggctcc
     4201 gtgcgccagc ggctgttgca gcaccggggt cgcgaaggtg ttgtcgacga gcacgggaac
     4261 ggtgcctgcc ctggcgacga cgtcggcgat gtcgacgagg tcgagggagg ggttggccgg
     4321 ggtctccacg atgacgagcc cggtgtcggg gcggatcgcg gcatccacct cgtcggtcct
     4381 cgcccaggtg accctggtgc cgagcagccc ggtggccagg aggtggtccg tcccgccgta
     4441 cagcggtcgc accgcgacga cgtggggacg gccggcggcg acggccgcga gcaggcaggc
     4501 cgtgatcgcg gccatcccgg aggcgaaggc caccccggcg ggcgctccct cgagctgggc
     4561 aagggcccgc tcgaaccggt cgacgttggg gttccacagt cgctggtaga ccagcgagtc
     4621 gtcctgctgg gggcggccac cggtggccat ccgttcgtag gcctcaccgc cgagctcgac
     4681 gctcgggagg ggataggtgc tcgactggtc gacgggtggg acgtgcgcac cgagggcggc
     4741 gaggtcctcc cgcccggcgt ggactgcgac agtgaggggc tccatgcctc gatggtggtg
     4801 ctttcaccgg atggggtgca acagttccgt ggaatcctcg acagatcgtg tgctgatggg
     4861 ggattcttcc gtacatggcc cctcacccga ccaccgacca ccggattccg cgccgccccc
     4921 tcgatgacgt cgaccgggcc ctggtcgagc tgctgtccac ggacgggagg atgccgaacg
     4981 ccgagctggc gcgacgggtg ggcatcgcgg agtcgacgtg cgccggacgg gtgaaggccc
     5041 tgcgtgatgg cggggtcgtg ctcgcgatcc gcggtgaggt cgacctcgcc gcactcggtc
     5101 gcccgatcga ggcgatggtg gcgctgcgct tcaccgggca cgcccgggag tcgatggact
     5161 ccttccgcga ccgggtggcg tccatcccgg gggtcgtcgc cgcctaccac gtcgccgggg
     5221 ccgacgactt cctcgtgcac atcagcggcg ccaccgccaa cgacctgcgt gacctcgtcc
     5281 tggaccacct cacctccgtc gagggggtgg ctcacgccca gacgagcctg atcttcgagc
     5341 gggtgcgcgg ccgtcggccg ctcgggtgag agcgaccggt cagcggtcgg tcagcgctcg
     5401 ccgacgggca cgtagttgcg ctcgccctcg ccgatgtaga cctggcgcgg ccgcccgatc
     5461 ttggtcgtcg ggtcctcgat catctcgcgc cactgggcga tccagccggg caggcggccc
     5521 agcgcgaaga gcaccgtgaa catgcgcgtg gggaagccca tcgacttgta gatcaggccg
     5581 gtgtagaagt cgacgttcgg gtagagcttg cgctcgatga agtactcgtc ggccagggcg
     5641 atctcctcga ggcgcatggc gatctcgagc aggtggtcac cgcccgccgt cttggcgagg
     5701 atctcgtgcg cggtgtcctt gatgatcgcg gcgcgggggt cgtagttctt gtagacccgg
     5761 tggccgaagc ccatgaggcg gacgccgtcc tccttgttct tgaccttggt catgaactcg
     5821 tcggtggaga agtcagcctt ctcgatgcgc tcgagcatct cgaggacggc ctggttggca
     5881 ccgccgtgca gcgggccgaa cagcgcgttg atcccggccg agaccgaggc gaagaggttg
     5941 gcctgcgagg agccgacgag gcgcacggtc gacgtcgagc agttctgctc gtggtcggcg
     6001 tgcagcacga gcagcaggtc gaccgcccgg acgaggtcgg ggtcgatctc gtagtccgtg
     6061 gccgggaccc cgaacgtcat ccgcaggaag ttctcgacga gggagaggtt gttgtccggg
     6121 tagaggaacg gctggccgat cgacttcttg taggcgtagg ccgcgatcgt cggcagcttg
     6181 gccagcaggc ggatcgtcga gatctcgacc tgctccttgt cgaacgggtc gaggctgtcc
     6241 tggtagaacg tcgacagggc ggagacggcc gaggagagca ccggcatcgg gtgagcgtcg
     6301 cgcgggaagc cctggaagaa gcccttgagg tcttcgtgca gcatcgtgtg ctggctgatc
     6361 cggccctcga actcggcgag ctggctcggc gtgggcagct cgccgtagat gagcaggtag
     6421 gaggtctcga tgaagctcga ctgcttggcg agctgctcga tggggtaccc gcggtagcgc
     6481 aggatgccgg cgtcgccgtc gatgtaggtg atggcgctgg tgcagctggc cgtgttggtg
     6541 aagccaccgt ccagcgtgac gttcccggtc tccttgagca aggacgagat gtcatacccg
     6601 ccgttgcctt cagtggcccg tacgagggac aggtcgagct gcttgccagc agcggacagg
     6661 gtggcgccat cagtcatgtc gtcgtccttc acgaggggca gggaaccatt cgaccttaca
     6721 gtccgtgggc gggagcagaa accgcacgcc cgtcagcctt gtctcgggag gtcacccgag
     6781 gcgacgaacg gcggcctcga tgcgctcatc ggtcgccgtg agggcgatgc gcacgtgctg
     6841 acgtccggcg gggccgtaga agtcaccggg ggcggcgagg atgccgcggt cggccagccg
     6901 ctcgatgctc acccggcagt cctcccccgc gctcgcccag aggtacaacc cggcttcgga
     6961 gtcatcgacc cgaaggccga aggactccac ggccggcagg agcgcggccc ggcgagcggc
     7021 gtaccgctgc ttctgcgcca cgacgtggtc agcgtcggac agggctgcga gcatggcgcg
     7081 ctgaaccggc cacggaacga tcatcccggc gtgcttgcgg acctcgagga gctgcccgac
     7141 cagaacgggg tcacccgcca cgaaggcacc gcggtacccg gcgaggttcg actgcttgga
     7201 cagcgagtac acgcacagca gcccctcgtg ggacccaccc gtcacgcgcg ggtcgaggat
     7261 cgacggcgtg gtcggcggct cgcgtgaccc cgcggcgcga gggcgccagt cgagctccgc
     7321 gtagcactcg tcggaggcga ccaccacccc gtgctcgcgg gcccaggaca ccaccttcgc
     7381 gaggtgctcg atgccgagca cctttccggt ggggttgccg ggggtgttca gccacagcag
     7441 cttcggcgtt gtcgctgccg tcagcgggcc cagtgccgtg agggcgtcga ccgccaccgg
     7501 tgtggccccc gcgaggcgcg cccccacgtc gtacgtcggg tacgccacgc gcgggaaggc
     7561 cacgacgtcg ccgggcccga tgcccatgag caacggcagc caggcgacga gctccttcga
     7621 gccgatcgtc ggcagcaccc ccccggggtc gacgtcgggc accccccgac gggccgcgaa
     7681 ccacgtggcc acggcttcgc gcaggtcagg agttccccag gtgagcgggt agccatgggc
     7741 atcggaggcg gcagcgaggg cgtcgcggac cacggcaggg gtgggatcga cgggggtgcc
     7801 caccgacagg tcgaccagcc cggagccggc cgggccgtcg atggtggggt cggaaaagga
     7861 gctcgcccgc tgcttggtcg cagcgagcga gtcccaggga aagtcgggca gggagagcac
     7921 cggcggtggc cgcgtcctac tcgtcgtgct cctgcggggg cagcgccgcg atgatcgggt
     7981 ggtccttggg gatcatgccg agcttggcgg ccccacccgg gctgccgagg tcgtcgaaga
     8041 actcgacgtt ggccttgtag tactccgccc actgctcggg gacgtcgtcc tcgtagtaga
     8101 tcgcctcgac ggggcagacc ggctcgcatg caccgcagtc gacgcactcg tcggggtgga
     8161 tgtagaggct gcgctcaccc tcgtagatgc agtcgacggg acactcctcg atacaggcct
     8221 tgtccttgag gtcgacacag ggctgcgcga tcacgtacgt catgacgccc atactatgcg
     8281 ggccatggac gagcgtgtcg aactgcccga gcgtgaaccc cgggtcgggt ctcgcgtcgt
     8341 ggtgcgacac accctgcccg aggcggaccc gctcaccggg gcgaccctca ccgacgtcgt
     8401 cggcgacctc gtcgagaccg atgccgcgac cctcgtggtc cgcacccgcc ggggcctggt
     8461 caccgtgccc cgccacctcg tgaccgccct caaggagatc ccaccgaccc cgtcgcgccg
     8521 gggcagcccc caccgggcgc tgtcgatcga ggacctgcaa cgcgtcatgg tcggcgcctg
     8581 gcccgcgatg gagagcgaac gcctcggcga ctgggtgctg cgcgcatccc gcgggttcac
     8641 ccagcgagcc aactcggtcg tgaccgccgg ctcccccgca cgaccgctgc ccgaggcact
     8701 ggatgccgtc gaacgctggt acgccgcccg tgacctgccc gccaacctca cgatcgccgg
     8761 ccccgtgggg ttcgacccgg catccgacct cgtgggtgcc gaggcgctga gacgcgggta
     8821 ccgcggccgg gtcgccaccg tgacgctcac cgcccccacc cgactcattg cacgccagcc
     8881 accccggaga gcggacaacg aggtggctgg cgtgcaagaa gtcgaggtgg ggcgtgacct
     8941 caccgacgac tggttcgccg cctaccgctc ctaccgcgcc gtggacgacc gcgccgcccg
     9001 ggtcgtgctc accggctcac ccgatcaggt gttcgcgacg acgagggatg ccgggggtcg
     9061 ggtcgtcggc atcggtcgcc tcggcctggc gtcggcctgg ggcggtatcg cggccatgtg
     9121 ggtcgagccg acgatgcgcc gccgcggggt cgcggacgcg atgctgaccg ccctcgccag
     9181 agcggccgag ggtgccggtg tcgcgtccct gcacctgcag agcgactccg acaaccacgt
     9241 ggccctggcg gtctacgggc agcgggggtt cgaccggcac cacgagtacg tcaacctcgt
     9301 gagcaccacc gcctgaacgg ctccgtcagg ggcgggggtt cgtgcaggtg acgtcatcct
     9361 cggggcggta gttcgtcgtg aacttctcgg tcttcacgac cttgcccttc tccttgatga
     9421 tccggccgac cgtgaccttg aagccgggga cagggctctg gtcgaggcag tcggggctgt
     9481 cgtcggtgaa ccgcctgggc tgcacgatgt cgaagcggtc gctcttggtc gcggtgacgt
     9541 cgtacttctt ggtgccccag aagttcacgg tgatcgagga gttggtcgcg atgacctcca
     9601 ttcgcacgcc gccgtcggtg gtgttggtga acttgttgtg caggtccggc cacgagatgg
     9661 tggcctcccg cccctccggg tagcgcgaga tgtagtagta gtgcggagtc caggcgtcga
     9721 gttccatgcc cgagaagaac gcagcgttga agatcgtcgt cgacacctgc gagatcccgc
     9781 cgccgtaggc gctcgcggcg cgcccaccgc tgatgatgcc tgccttgacg tagcccttgt
     9841 caggagtgcg cttgccgagg atggcgttca tgctgaactg cccccccggc gggatgtacg
     9901 tgccgttgag gactcgcgac gcgaccttga tgttcgtgac gcgcggctgc cctgccgggt
     9961 agtaggtggt gaaggaggag atcttctcct tcggcagggt ggccttggcg atcgcggtcg
    10021 tgaacttcgg cgcgacggcc ttggtggtga ccgtggcagt gcgggtcggc gtcgtgatcg
    10081 ccttccacac ctcggtctgg accgaggcgt cgtcgagacc gacgccggga acgtccgggg
    10141 tcaccgtggg cgtgctgccg gagaaggtca cgacagcgtc cttggccacc acctcggcgc
    10201 cggccttctt cgccgcagca ctcacgatcg cggacagctt cttggcgtct gccttggggg
    10261 tgatggtgcc cgagtcatcg gctgccagga cgatggcagg agcgaaggcc ttcggggtga
    10321 gggtgaagga cttctccccc gccttcacgg tcaccgggcc ggacacggcc acgtcggcga
    10381 actcggtctt gacccgggcc agctcctgcg cggagacctt cggctccagg ctgcgggcgg
    10441 caaccttgat ggtgctctgg gtcggccacc agcgctgcac ggcggccacc gtgcccgcga
    10501 cgtcggtctc ggtgcccgtc gtcggttcct tcagggcaac cttgccccct gcgacggaga
    10561 tcgacccctc gacgggctcg gagtccaggt cggaacgggc cccctccacc gccgtggtga
    10621 acacggcctc gtcgaccgtg acgacagcgt tctcctcgcc accaccgaag aggtttcgcc
    10681 acacgtgggc cggggcgagg gagaagccca cgagcccgtc gaccgtggtg tcgatgtcga
    10741 cgctcacgcc gagatcggaa ggctggacgg cgacctcacc cacgggcgag gtcagggtga
    10801 ccggctcggt gccgacatcg gccaagcccg cagccaaggc gtcccgagcg ccatcgacgt
    10861 ccttgccgcc cacgtcgacc cccgcgaccg tggtgccccg tggcacgcgg tcaccgagaa
    10921 ccacggccgc agccacgtag gcggccacga gcgccacgag ggcgccggcg agcaggaatg
    10981 gccaccggcg ccgggacgac ggctgcgcac tcccgtggtc atccgccgag gaggtctgcg
    11041 cgaacaggtc ggtcatgcga tgctcccgta gcttccgcgg aagtggacca gggctggccc
    11101 cacgtcgtcc ggcaaggtga tggacaggac ctcggcgacg acgatcatgt ggtcgccggc
    11161 agcgtggctg gtggtgacgc ggcactccag gtgcatgagt gcgccgtcga gcagcgccat
    11221 gtccgtgtgt ggaccacgac ggtgcggtgc ccggtcgagc tgcccgtgca gcgggcgacc
    11281 gggggtggcg aaccactgcg ccagtgctcg ttggtcggcc gcgaggacgc tcacgccgaa
    11341 gacccccgcc tgcgcgaccg cagccagcca ccgggtctcg ttctcgacac agacgagcac
    11401 caggatcggg tcgagcgaca ccgaggtgag ggtgtctgcg gtcatggcgt ggtcgtggcc
    11461 gtcgctggtc gtcgtgagca ccgtgacgcc agacgcgagg cggcccatcg ccaggcggaa
    11521 ctgggctgga tcgaggtcgt ggtggctcac gctgattcct cctgctcggg gagctgtccg
    11581 gtcaagggat cgagccgacc gtatccctcg cgtcccgcga gccgggacac gacgtggttc
    11641 gacaaggcat accagccgtc gccgacgacg acctgggtct cgtggcagcg cagggcctgg
    11701 ctctgggccg cgacgacgga ggggtcgagc accgcgtggg tcaccacggc atccgggacg
    11761 accgacgacg cgaaggggtc gtccggcccg ggcacgatga gtccgcgatc gcccggcacg
    11821 tgctccgcga gccactcccg gtcctgcagg acccaggact gcggcgtcag cgtgacgtag
    11881 aggacgggac gcacggccgt gcctgcgacc gcgcgcaccg tcgcgtcgtg ggtgcgcacg
    11941 tggtcgggat gaccgtagcc accggtcgcg tcgtaggtca cgacgacgtc ggggcgtacc
    12001 tcgtcgatca cggcccggat ggcggcggcc gcctcctcga ggtcggcgcc gacccaggcg
    12061 cgcgggtgct cggcagcagc ggatcctgcc atgccggagt cgcggtaggt cgcagcggca
    12121 tcgccggacg aggcgccgag caggtggtgg acgacgccca gggaacgcat ggctgccgtg
    12181 agctcaccgc ggcggtggtg ggcaagcggg tcccccgccg caccctcgag gtgggccagg
    12241 ggtgcgggga tgacctcgcc ctcctcgccg agggtgcacg tgaggacgtg gacgtcatcg
    12301 ccgcgcgcga cgtggtgggc caaggccacc ccggtcgcga gggtctcgtc atccgggtgg
    12361 gcgtgcacga agagcagccg ggggcgctgc ccgcggatcg ccacgtcgtc gaggaggcct
    12421 ttcacgggcc caccgcgatg tccgcgaact cgacgacgcc tccggcgatg gtgtttgccg
    12481 acaggttgcg gatgtccgat cccgcgacgt agagcgcgcg ccgctccgcc agcccgatgt
    12541 acgccccctg ctcgagcagt gactggtcga cgacgaccca cgccgcctca cgctcggccc
    12601 ggtcgtcgat ctgctggatc tgcgccatcc ggctgttcag cttggggtcg gcccagtagc
    12661 cgtagtcgcg cccgggcccg gacgccgtga tgttgatcgc gctgtcgaac aggggcggca
    12721 ggatcgtgga tgcagacgcc caggccggag cccagttgct ccagaagaca tcgacctcac
    12781 cggcgatctt ggggtcgctg atggcggtga agtagtcatc ggtgatgggt ctggtcgtgg
    12841 gctcgaaccc agccagccgc cagccggcga ccagggccgc catcgccttg tccgaggtgg
    12901 cgctgctgcg gtaggccacg gtgaacggca ccggctcggt ccgcttggcc tgggcgagca
    12961 gcgccttggc cgtggcagcg tcgccacggt tgcccgcgcc cacggggtcg ccttcgtgcg
    13021 cagccgccag ggagcgcggg atgagcgagc gtgccgggtc tgccgccgtc ggcccaccga
    13081 tcgccgtcac gtaggcctcc cggttggtcg cggcggcgag cgcccggcgg accagggggt
    13141 ccttgaagac cgcgctcctg gtgttgggaa ccaggtagtc gacgacccct gtccgcgggt
    13201 tgaccgaccg ctcgcgcagg ctctggaccg cggtgatgtt ctgctggatc gccggcgggg
    13261 ccgaactcag ggagaccgag gtgcgaccac cgtcggtgtc ggccatcacc tgctgggtga
    13321 tggcctggat gtccatgccc tcgacgtact cgatgcgatc gggatgggct cggcgcaccg
    13381 ggtcggctga cctggcccag tgcgggttgc gcacgaacgt gccgccctcg ccgggcaccc
    13441 acggcttctc gagcacgtag ggaccggcgg agaacacctc gtacacaccc tcggggccct
    13501 tgtcgacgga cttcttgacc ggcccgaagg caggttggct gaccagttcg ttgaagtcgc
    13561 cgagcggagt gctcagcgtg aacgtgagct tctgcccgac gcacgacacg gccttgtcga
    13621 atgccgcctg ccccgccgcc gcggccgggc ccgtggcata gggcccggca tacgtgctcg
    13681 tgccgttggc ctgcctcggg atggcgagga cggccagggc atccgtggag ccacccggca
    13741 ggtccttgct cgcgaagctg cgggagatgc cgtaggcgac gtctgcacag gtcacggggg
    13801 tgccgtcctg ccagaggatg ccctcgcgca gggtgaacga ccaggtcttc aggtcatcgc
    13861 tggcggtgcc ggtgtcggtc gcgaggtcac cgaccaggcg atcctgtgcg tcggggtcga
    13921 cgttgggtgc gtacgccgtc agcgtgcggg cgaacacccg accggcgaac gcgatgtcgt
    13981 cgcgcgaggc cagccgttgc gggtcccagg tgagcaccgg gcccagggcg agtgcccgga
    14041 tggtgccgcc gtcccggttg acccgctcac cgacggcgga ggtcgccgtc gcccgatccg
    14101 ggtcgaccgt gggctcgtca cgcaggacga acaccccggc cacgacgagg gccgccacga
    14161 ccagggtgcc gagcacgacg gccgcgaggc cagcgtgccg gtgagcaacc ggcatccgaa
    14221 gccgccgttg cgcgtgccgt tcggcacgcg tgcgtctcgg ttcctgcgct ccgccgtcat
    14281 tcgcggacac gttcggtcag ctcttcctcg cgcgggcggc ggcgcgggcg cgcagcgtct
    14341 ggtcgagctc gaccttgcgg atgcgaacag cctccggggt cacctcgacg cactcgtcct
    14401 cgcggcagaa ctccagcgac tgctcgaggg agagcgcccg cggcggggcg agccgctcga
    14461 ggacgtcagc accggcggag cgcacgttgg taagcttctt ctccttggtg atgttgacgt
    14521 ccatgtcgtc cacgcgggag ttctcgccga cgatcatgcc ctcgtagacc tccgtcgtcg
    14581 gggcgatgaa gagggtgccg cgctcctgga ggttcatcat cgcgtaggcg gtcgccgggc
    14641 cggagcggtc ggcgacgagc gaaccgctga tccgggtgga gatctcgccg acccagggct
    14701 cgta
//
LOCUS       DAOSOC010000046        14202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4309_length_14202_cov_10.251361, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000046 DAOSOC010000000
VERSION     DAOSOC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4309_length_14202_cov_10.251361"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2627)
                     /gene="asnB"
                     /locus_tag="PL014_05420"
     CDS             complement(<1..2627)
                     /gene="asnB"
                     /locus_tag="PL014_05420"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOT56564.1"
                     /translation="MCGIAGYHGLPADPALLERMNAHQQHRGPDGDGICVDGPCGLAH
                     RRLSIIDIAHGQQPMDTADGRYAIAYNGEVYNYLDLRGELEALGRTFTTDSDTEVVLQ
                     AFAQWGADAFDRFNGMFGLAIWDRHEQRLTLARDHFGIKPLYVAMVPNPSGEGEALLF
                     SSEIKPILASGLYEKRVNDRSVYRYLRFRAHEDGTETFFEGIERLAPGEMLEADANGI
                     RRRMFTRLKEELLELASVQRPYDAAAAAEYKRRLVESVRLRLQSEVPVGTSLSGGLDS
                     SAVAVIINQLLNEGDSLSLSAVGARQNTFSAIFPGSINDEEKYVDDVLDICTGHVEAH
                     KILPTADEFKADLLDFIRTQEEPIISSGPYAQYQVMREATKHVTVLLDGQGADEMMAG
                     YIPYYFVYLRQLRAQGATLAAAELAKSLDVLYRLGRFKLKAKVKLKKSIPTTHLLNRD
                     FVAAHRGERYSSEGANLKKRLVEDLFHNSLPSLLRYEDKNTMRFSLEGRVPFLDKEVL
                     KFIFSLSDEAIIKDGWNKRVLRDATRGLLPESINRRRNKIGFTTPQGEWFMRLKNHFY
                     NIFLSESFANRPYVNQTEVIAAFEGWIKGANDVDTMTFWRLINLELWMREFIDEHEDA
                     GDATDATARVKTDYEANEGQNLDLVTSLGENVRRYPVRTELFSRDDDLDARVLEHIDA
                     FFAGLPAAGPDHEAATRGPWWFFLSEKVVAITQGRSYFVWDIKVGRPARVLSRYVTRT
                     PAGIGLGSPFTMQLAIQEAGLPRVLYASAGGAIGKVIGRKGLFYELVGNQINAIDGPT
                     EYSAYPSNVSAKLAPKDPDAVAARLSAAIRERVPEPWRSTFGGTVIMDANDIGRNVLG
                     SDVPGDWTRFEEMFADNPLG"
     gene            complement(2740..3663)
                     /locus_tag="PL014_05425"
     CDS             complement(2740..3663)
                     /locus_tag="PL014_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOT56565.1"
                     /translation="MQSTTTTVTASDGTALHTHRWVPDGEPKAVVQIAHGMAEHSARY
                     ARLAQALTEHGYAVYAHDHRGHGSTASEIDHGYFADENGWDAVVADMRAVTRLAQEEN
                     PGLPVFLLGHSMGSFLARTYVIENSRDLAGLVLSGTGGDPGPLGKVGLGVARLEKRVR
                     GPRHVSALLDKLTFGQFNAAFKPNRTGFDWLSRDDAEVDAYVDDALCGRTFTSGFFVD
                     LFGGIQRINDRKQVARVRRDLPILLIAGDKDPVGDGGRGPRAVAEQYSSVGVVDVTCT
                     LYPGARHEIFNETNRDEVTADVVAWLDSHLP"
     gene            complement(3998..4753)
                     /locus_tag="PL014_05430"
     CDS             complement(3998..4753)
                     /locus_tag="PL014_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HOT56566.1"
                     /translation="MKVTSTTRRVLAAAAVVAAAVATQAAAVPASATSSTLTCANVIT
                     IVARGSDEAAGTGGTTNVYSSGGLGLMSGVAGKVTGGTSKSVRTVGLKYPATIGFGGL
                     AYFSSLDTGKARLAAELNRLATLCPSSRTVLIGYSQGAHVVGDVTSNSNPNALSTGAK
                     SRIAAVYLTGDPVRRPYESHNRGTGIGGGVLVDRGAGQMSGLGSRILSYCYKGDWACD
                     APHKDPTRSAAIHTSYGNSTLNTTYGTWIVGKL"
     gene            5028..6209
                     /locus_tag="PL014_05435"
     CDS             5028..6209
                     /locus_tag="PL014_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56567.1"
                     /translation="MTDAQTGTDAATLPRAQRAIGLFVAGLRLATLAQMLPSLVAALD
                     QGEHRALTALTWAVASAMALATAAVCVVRRRPPGTAWACLDTAVAVGLLFVGLVTVPV
                     ELRTGSWIGFQSAYSLSVAGSLVGVRDVRIWGALVGTLVAARAVYVLPIISTAADVPT
                     VVGELFTMLGLAPLAWFGVGTVLRIAQDADESRAYAARLAREDEERKARAAIHNGTAL
                     MKILVDQGALGAVDSRTPSQVWVQAATELNRMRAYLAGGPGQTGEEPHDLAELVENIA
                     TEFTDLPLDVVPDLARGTTLGAEATDLATALRSILINVRQHANATRVVLHAEELEDTP
                     GWAVTVHDDGVGFDTRETTYGVGIRTLTLDQLSPHGIETTIDSVPGVGTTVTLTREGD
                     R"
     gene            6206..6901
                     /locus_tag="PL014_05440"
     CDS             6206..6901
                     /locus_tag="PL014_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HOT56568.1"
                     /translation="MTDPDQSSDVRFSLVDDATAVRESLAVLLPDLTFVSSHPSLEDF
                     LAAAPRADVVILDLHLVNLRQPDAAQGVAAIHAAVDAGYRVCVYTQEERRFVLASCLA
                     AGATGVVSKSSTLAETRTAFLAVSRGELVAPPSVTGLIEALVRRGRVTVLNKRQREVL
                     AGRARGLTYAELSRTLYLSESTLRGYWRDLSGVVSSYLQDSSPGDIERALGLGPGDLV
                     DHWPTRSPADDLD"
     gene            complement(6916..8523)
                     /gene="guaA"
                     /locus_tag="PL014_05445"
     CDS             complement(6916..8523)
                     /gene="guaA"
                     /locus_tag="PL014_05445"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT56569.1"
                     /translation="MSESPSTQPESTHLADRPVLVVDFGAQYAQLIARRVREANCFSE
                     VVPHSMPVAEMLAKDPAAVILSGGPSSVYEDGAPSLDPALLQAGVPVFGMCYGFQAMA
                     QALGGTVERTGLSEFGATPATILDSTSTLFNGQPVEQSVWMSHGDSVSAAPEGMTVTA
                     TTSGAPVAGFEDDERHLYGVQWHPEVLHSTFGQQVLENFLLRGAGLTGEWTAANVVDD
                     LVSSVREQIGDARVICGLSGGVDSSVAAALVQRAVGNQLTCVFVDHGLLRSGEAEQVE
                     QDFVAATGVDLVVVDAKERFLTALAGVSDPEEKRKIIGREFIRVFEQAARDVVGSHAG
                     SDDHPVEFLVQGTLYPDVVESGGGTGTANIKSHHNVGGLPDDLQFKLVEPLRLLFKDE
                     VRQVGLELGVPEAIVWRQPFPGPGLGIRIVGEVTGERLDILRAADLIAREELTAAGLD
                     RSIWQCPVVLLGDVRSVGVQGDGRTYGHPIVLRPVSSEDAMTADWTRVPYEVLAKIST
                     RITNEVREVNRVVLDITSKPPGTIEWE"
     gene            complement(8533..8901)
                     /locus_tag="PL014_05450"
     CDS             complement(8533..8901)
                     /locus_tag="PL014_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOT56570.1"
                     /translation="MTTPDSRTHTIDDVEGARSRLTFFKVMAFIVGVGLLVLVVEMVL
                     SYGFGLKGEDNPLYWWPQPHGFIYMVYLVATALLGFKIGWSLTKMVLVMLAGCVPFLS
                     FWVERRVARQVESELAAARQ"
     gene            complement(8888..9643)
                     /locus_tag="PL014_05455"
     CDS             complement(8888..9643)
                     /locus_tag="PL014_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOT56571.1"
                     /translation="MLRTALRPRFLGLLGLVVVVGIAFAFLGRWQLGVAESRSYREAV
                     EQARAQEPAALTSVLEPHAPFRGDLSSRPVTAQGSYAPDGQLLVPDRRLDGVSGSWVL
                     TPFVVEGSGATIAVVRGFVTNPADAGAPPSGPLTIAGGLAPGESPSSVPVAAGEIGSI
                     DLSLLVNTWPGELYNAFVFLESETPQAAGQPTRVPTPLPDTSIQWRNAAYAVQWWVFA
                     LFALWVWFRMVREETRREALLEAPGDTGSRDDA"
     gene            9715..11451
                     /locus_tag="PL014_05460"
     CDS             9715..11451
                     /locus_tag="PL014_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56572.1"
                     /translation="MSPHPWPYATAPDAAPPLDADRLILWLARHQWRTLLGGVLFGVP
                     WMLSIALVPAAIGKAVDDGLVARDPRALVTWAAAIMGLGLVSAVTTNGRHWFAVRNWL
                     ISSFRTAVVTEGAVRGAGPAVTRQMPAGEVVTSFASDFWRMGEVFDVTARLAGAIVSF
                     IVVSAILLRGSVLLGVIMLVGGPLLLASLALVVRPLHRRQQAQREEAGQLTALGADTV
                     AGLRVLRGIGGEDTFLARYAAQSERVRLAGVRLSPVQATLDAAQVLLPGIFVVVVTGI
                     GAHLAVGGEITPGQLVAFYGYTAFLTMPLRTATEFVDKLTRTRVAARRIVKILAIEPD
                     HAPAGRTSGDLLVDGGTPVVDVSADLVDPASGVVVVPGQFTALVSADPEDTAQIAQRL
                     GRLLPGRHGVRWGEVDLDDLPIDRVRSLVVVSGADPHLFSGPVRDALGGADDAAREVA
                     VHVASASDAIDSLDGGLDGELEERGRALSGGQRQRLALARAVLRDPEVLVLVEPTSAV
                     DAHTEARIAQRLAARRAGRTTVVVTASPLLLDTADTVLFVQDGAATATGRHRDLLRTH
                     PAYRDVVLRGED"
     gene            11451..13226
                     /locus_tag="PL014_05465"
     CDS             11451..13226
                     /locus_tag="PL014_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56573.1"
                     /translation="MSPLLGRTDHAARTLPIATTATVRAHSKELLRHHRGPLTWVLLW
                     HGIAALVALVGPWVVGQVVQEVTTGSPSLRRVDVLIGLLVAAILTQTVLTWVARRASF
                     VLSERIFARLREDFITSSVRLPLSTVERAGTGDLVARTTNDIDSLAHIIRFGIPAIVV
                     AVTTVTATAVAALLTSPLAALALLTAVVVHVPTSRWYLRRAGDGYLWEHASYARLNGV
                     ASETIEGAGTMDALGLQTARGRRFQEAMDECGDAEAYTLGLRLRWFPWLEIGFFLPIA
                     TSVLWGGWLAWEGHISIAAATAVTLYVQQLLDPLGELISWLDEIQFAATSLARILGVA
                     EVPPDRVASGAQPVGEQMEVDDVRYSYRQGRDVLHGVSLRLEPGERLAIVGPSGAGKS
                     TLGRLLAGIDGPRTGRVTGGGVPLVDLELPVLRGEVALVTQEHHVFVGTVAENLRLPR
                     PEATDDELHEALAAVDAAGWAAALPHGLDTVVGSGGHPLTEAQGQQLALARLVLADPH
                     TLVLDEATSLLDPRAARHLERSLAAVVDGRTVVAIAHRLHTAHDADRVAVVEDGVISE
                     LGSHDELIARDGSYAALWRSWRDEA"
     gene            complement(13243..13926)
                     /locus_tag="PL014_05470"
     CDS             complement(13243..13926)
                     /locus_tag="PL014_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016996070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOT56574.1"
                     /translation="MQAVTEPVIDLIESVGELGVGALLALETVLPPIPSEVILPFAGY
                     AAATGRLDPVLAWAAATVGSVVGAFILYGLGRALGYDRLLDLSRRRWFFLLSTADLNR
                     GHRFFAKHGRAVVFFGRFIPLVRSVVSVPAGIERMPAPRFAALTAAGSGIWNALFIAA
                     GYQLGENYAVIEQWIAPVSTGVVVALAAGLVWLVVRRRRAVDGESSDVVHSDSSSKPA
                     EPELELTSR"
     gene            complement(13996..>14202)
                     /locus_tag="PL014_05475"
     CDS             complement(13996..>14202)
                     /locus_tag="PL014_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HOT56575.1"
                     /translation="VDELVGDATGGFGSVRVEVTVGSSTWLTSLFPSTEQGAYILPVK
                     KAVRRAEGVGDDDPFEVTIRLVGR"
BASE COUNT         2032 a         5092 c         4994 g         2084 t
ORIGIN      
        1 ccgagcgggt tgtcggcgaa catctcctcg aaccgggtcc agtcacccgg cacgtcagag
       61 ccgaggacgt tgcggccgat gtcgttggcg tccatgatca ccgtgccgcc gaaggtcgag
      121 cgccacggct cggggacccg ctcgcggatc gcggcactga ggcgcgccgc cacggcatcc
      181 gggtccttcg gggccagctt cgccgagacg ttcgacgggt aggcggagta ctccgtcggt
      241 ccgtcgatgg cgttgatctg gttgccgacg agctcgtaga acaggccctt tcgcccgatc
      301 accttgccga tcgccccccc ggccgatgcg tagagcaccc gcggcaggcc ggcctcctgg
      361 atcgcgagct gcatcgtgaa cgggctgccg aggccgatcc ccgcgggggt gcgggtcacg
      421 tagcgcgaca gcacgcgcgc cggtcgaccg accttgatgt cccagacgaa gtaggagcgg
      481 ccctgcgtga tcgcgacgac cttctcggac aggaagaacc accacggtcc gcgcgtcgcg
      541 gcctcgtggt cggggccggc ggctggcagc ccggcgaaga aggcgtcgat gtgctcgagc
      601 acccgtgcgt cgaggtcgtc gtcgcggctg aacagctcgg tgcgcaccgg gtagcggcgc
      661 acgttctcgc ccagggaggt cacgaggtcg aggttctgcc cctcgttcgc ctcgtagtcg
      721 gtcttgaccc gggcggtcgc gtcggtggcg tcgccggcat cctcgtgctc gtcgatgaac
      781 tcacgcatcc acagctcgag gttgatcagg cgccagaagg tcatcgtgtc gacgtcgttc
      841 gcgcccttga tccacccctc gaacgccgcg atgacctcgg tctggttgac gtacggccgg
      901 ttggcgaagg actcgctgag gaagatgttg tagaagtggt tcttcagccg catgaaccac
      961 tcaccctggg gggtggtgaa gccgatcttg ttgcggcggc ggttgatcga ctcggggagc
     1021 aggccgcggg tcgcgtcgcg cagcacccgc ttgttccagc cgtccttgat gatcgcctcg
     1081 tcggacaggc tgaagatgaa cttcagcacc tccttgtcga ggaacggcac gcgcccctcg
     1141 agggagaagc gcatcgtgtt cttgtcctcg tagcgcagca ggctcggcag cgagttgtgg
     1201 aacaggtcct cgacgaggcg cttcttgagg ttggctccct ctgagctgta gcgctcgccc
     1261 cggtgcgcgg cgacgaagtc acggttcagc aggtgcgtgg tggggatcga cttcttcagc
     1321 ttgaccttgg ccttgagctt gaagcggccc agccggtaga gcacgtcgag gctcttcgcg
     1381 agctcggcgg ccgcgagcgt ggcgccctgg gcgcgcagct gacgcaggta gacgaagtag
     1441 tacgggatgt acccggccat catctcgtcg gcgccctgcc cgtcgagcag gacggtgacg
     1501 tgcttcgtgg cctcgcgcat cacctggtac tgcgcgtagg gcccggagga gatgatcggc
     1561 tcctcctggg tgcggatgaa gtcgagcagg tcggccttga actcatcggc cgtcggaagg
     1621 atcttgtgcg cctcgacgtg cccggtgcag atgtccagga cgtcgtcgac gtacttctcc
     1681 tcgtcgttga tcgacccggg gaagatcgcg gagaaggtgt tctgccgggc gccgaccgcg
     1741 ctgagggaga gcgagtcacc ctcgttcagc agctggttga tgatgacggc gaccgcactc
     1801 gaatccaggc caccggacag gctcgtgccc acgggcacct cggactgcag gcgcagccgc
     1861 accgactcca cgaggcggcg cttgtactcc gcggccgcgg ccgcgtcgta ggggcgctgc
     1921 acggaggcga gctcgagcag ctcctccttc agccgggtga acatgcgacg tcggatgccg
     1981 ttcgcgtcgg cttccagcat ctcgcccgga gcgaggcgct cgatgccctc gaagaaggtc
     2041 tccgtgccgt cctcgtgggc gcgaaagcgc aggtagcggt agaccgagcg gtcgttgacc
     2101 cgcttctcgt acaggccact ggccaggatc ggcttgatct cgctggagaa cagcagcgcc
     2161 tcgccctcac cgctcgggtt cgggaccatg gcgacgtaga gcggcttgat gccgaagtgg
     2221 tcgcgggcca gggtcagccg ctgctcgtga cggtcccaga tggcgaggcc gaacatgccg
     2281 ttgaaccggt cgaacgcgtc ggcgccccac tgcgcgaagg cctgcagcac gacctcggtg
     2341 tccgagtcgg tcgtgaaggt gcgaccgagc gcctcgagct cgccgcgcag gtcgaggtag
     2401 ttgtagacct cgccgttgta ggcgatcgcg taccggccgt cggcggtgtc catcggctgc
     2461 tggccgtggg cgatgtcgat gatcgacagc cgccggtgcg ccaggccgca gggcccgtcg
     2521 acgcagattc cgtccccgtc agggccacgg tgctgctggt gggcgttcat gcgctcgagc
     2581 agcgcggggt cggcggggag cccgtggtat ccggcgatgc cacacatgcg tctggtcggt
     2641 cctcacgtat cggggctggc ggtggtgccc acccagcctg ccacggatgc cgcccgtgtc
     2701 cgattccatc ggctccctcg gggcgagccg gcgcctctct cagggcaggt gggagtccag
     2761 ccaggcgacg acgtccgccg tgacctcgtc acggttggtc tcgttgaaga tctcgtggcg
     2821 agcacccggg tacagggtgc aggtgacgtc gacgaccccg accgagctgt actgctcggc
     2881 cacggcgcgc gggccccttc cgccgtcacc gacggggtcc ttgtcgccgg cgatgagcag
     2941 gatcggcagg tcgcggcgca cccgtgcgac ctgcttgcgg tcgttgatcc gctggatccc
     3001 gccgaagagg tcgacgaaga agcccgaggt gaaggtgcgc ccgcagagcg cgtcgtcgac
     3061 gtaggcgtcg acctccgcgt catcgcgcga gagccagtcg aacccggtgc ggttgggctt
     3121 gaaggccgcg ttgaactggc cgaaggtcag cttgtcgagc agcgcgctca cgtggcgggg
     3181 gccacgcacc cgcttctcga gccgggccac accgagaccg accttgccga gggggcccgg
     3241 gtcgccgccc gtccccgaga gcacgaggcc cgcgaggtcc cggctgttct cgatgacgta
     3301 cgtgcgcgcg aggaaggagc ccatcgagtg cccgaggagg aagaccggca ggccggggtt
     3361 ctcctcctgg gcgaggcggg tcactgcacg catgtccgcg acgacggcat cccacccgtt
     3421 ctcgtcggcg aagtacccgt ggtcgatctc gctcgccgtc gagccgtgcc cgcggtggtc
     3481 gtgggcgtag acggcgtacc cgtgctcggt gagggcctgc gcgagccgcg cgtagcgggc
     3541 cgagtgctcg gccatgccgt gggcgatctg gacgacggcc ttgggctcac cgtccggcac
     3601 ccaccggtgg gtgtgcaggg cggtcccgtc gctcgccgtg accgtggtcg tcgtgctctg
     3661 catcggtgcc gcctttggtt acctgtgagt atgccgggga cagcctgcca cgtcgcgggc
     3721 gcgaggggca gtgacccacg cctcagcggg acggcccacc agctcggcgg cagtcgacgc
     3781 agacggcgag cggcggtggg actggggcac ggcggaggtg gcgggaccct gcaccgttgg
     3841 gcacggaccg gccaccggca ggatctgccg gtggccgggc cagcactcac ccctcctgcc
     3901 tcaccagcga accggcgagg agtcctgagg accatcgtgg tgcacgggtg ggcgtcacgg
     3961 catccaacga atccgcggag ttgcccatct cccaggctca gagcttgccg acgatccagg
     4021 tgccgtaggt cgtgttgagg gtggagttgc cgtagctggt gtggatcgcg gcgctgcggg
     4081 tggggtcctt gtggggcgcg tcgcaggccc agtcgccctt gtagcagtag gagaggatgc
     4141 gcgatcccag accactcatc tgccccgctc cgcggtcgac gaggaccccg ccaccgatgc
     4201 cggttccccg gttgtgcgac tcgtaggggc gccgcaccgg gtctcccgtg aggtagaccg
     4261 cggcgatccg gctcttggcg ccggtgctga gcgcgttcgg gttgctgttc gacgtgacgt
     4321 caccgacgac gtgtgcaccc tgcgagtacc cgatgagcac ggtcctggag gaggggcaca
     4381 acgtggccag ccggttgagc tccgctgcga gacgggcctt gccggtgtcc aggctggaga
     4441 agtacgcgag gccaccgaac ccgatcgtgg ccgggtactt cagcccgacc gtgcgcaccg
     4501 acttcgaggt gccacccgtc acctttcccg caacccccga catcaggccg aggccacccg
     4561 acgagtacac gttcgtcgtg ccgccggtcc cggccgcctc gtccgacccg cgcgcgacga
     4621 tcgtgatgac gttggcgcag gtgagcgtgg agctggtggc ggatgccgga acggcagcgg
     4681 cctgggtcgc caccgctgcg gcgacgacgg ctgcagcggc caggacgcgt cgcgtcgtgc
     4741 tggtgacctt catggtgtcc tccttcgaac gaccggtgcc ggcccggtgt cgggcagtca
     4801 tcacggttcg ccgcccatcg ggccggcgag tggccagcgt cgccgcgcgc ggcccgcacc
     4861 ggcatcccac gaaaccgcgg aaacggactc ctggatccaa cgaaaacgcg gaactcgagc
     4921 ggattgcccc tctgtgcccc cgcactgccc tgagcaccct gcggcgtggt ggaggatgca
     4981 gaggacgacc gaacgcacgg gacccgggga ggtgagcgat gaccgtcatg accgatgccc
     5041 agacaggcac cgacgcggcg accctgccac gggcccaacg ggccatcggc ctcttcgtcg
     5101 ccggcctgcg cctcgccacg ctcgcccaga tgctcccgtc cctggtcgcc gccctcgacc
     5161 agggtgagca ccgggcactc accgcgctga cgtgggcggt cgccagcgcg atggcgctgg
     5221 ccaccgcggc cgtgtgcgtg gtgcgacgcc gcccaccggg tacggcgtgg gcgtgcctgg
     5281 acaccgccgt cgccgtcggc ctgctcttcg tcgggctcgt cacggtgccg gtcgaactgc
     5341 gcacggggag ctggatcggc ttccagagcg cctactcgct ctcggtcgcc ggctcgctcg
     5401 tcggggtgcg cgacgtgcgc atctgggggg cactggtcgg taccctcgtg gccgcccgcg
     5461 ccgtgtacgt gctgccgatc atctccactg ctgctgacgt ccccacggtc gtcggtgagc
     5521 tgttcaccat gctcggcctc gcgccactgg cctggttcgg tgtcggcacc gtgctgcgca
     5581 tcgcgcagga tgccgacgag tcccgggcgt acgcggcccg cctcgcgcgg gaggacgagg
     5641 aacgcaaggc ccgggccgcg atccacaacg gcaccgcgct gatgaagatc ctcgtcgacc
     5701 agggcgcgct cggcgccgtc gacagccgca ccccctcgca ggtctgggtc caggccgcca
     5761 ccgagctcaa ccgcatgcgt gcctacctgg ccggcggccc cgggcagacc ggggaggagc
     5821 cccacgacct cgccgaactc gtcgagaaca tcgcgaccga gttcaccgac ctgcccctcg
     5881 acgtcgtccc ggacctggcc cggggaacca ccctgggcgc ggaggcgacc gatctcgcga
     5941 cggcgctgcg cagcatcctc atcaacgtgc gccagcacgc caacgcgacc cgggtcgtgc
     6001 tgcacgccga ggagctcgag gacacccccg gctgggcagt caccgtgcac gacgacgggg
     6061 tcggcttcga cacccgtgag acgacctacg gagtcggcat ccgcaccctg accctcgacc
     6121 agctgagccc gcacggaatc gagaccacga tcgacagcgt ccccggagtg ggcaccaccg
     6181 tcaccttgac gcgagagggt gaccggtgac cgaccccgac cagagttccg acgtccgctt
     6241 cagcctcgtc gacgacgcca ccgccgtgcg cgagtcgctg gccgtcctgc tgcccgacct
     6301 caccttcgtc tccagccacc ccagcctcga ggacttcctc gcagcggccc cgcgcgccga
     6361 cgtcgtcatc ctcgacctgc acctggtcaa cctgcgccag cccgacgccg cgcagggagt
     6421 ggctgccatc cacgccgcgg tggatgccgg gtaccgcgtg tgcgtctaca cccaggagga
     6481 gcgccgcttc gtgctggcct cgtgcctggc ggccggcgcc accggggtgg tgtcgaagtc
     6541 gtcgaccctg gccgagacgc gcacggcgtt cctcgccgtg tcgcgcggcg agctcgtggc
     6601 accgccgagc gtcaccggcc tcatcgaggc actcgtgcgg cgcggacggg tcacggtgct
     6661 caacaagcgc cagcgcgaag tgctggccgg gcgggcccgc gggctcacgt acgccgagct
     6721 gagccggacg ctgtacctgt cggagtcgac gctgcgcggc tactggcgcg acctctccgg
     6781 tgtggtgtcg agctacctgc aggactcctc gccgggcgac atcgaacgag ccctggggct
     6841 cggccccggt gacctcgtcg accactggcc gacgcgctca ccggccgacg acctcgactg
     6901 agtggaggct gccggtcact cccactcgat ggtgcccggc ggcttggacg tgatgtccag
     6961 gaccacccgg ttgacctcac gcacctcgtt ggtgatgcgg gtcgagatct tggccagcac
     7021 ctcgtaggga accctggtcc agtcggccgt catggcgtcc tccgacgaca ccggccgcag
     7081 cacgatcggg tgtccgtagg tgcgcccgtc accctgcacc ccgaccgagc ggacgtcgcc
     7141 gagcagcacg accgggcact gccagatcga gcggtccagc cccgccgcgg tcagttcctc
     7201 gcgggcgatg aggtcggccg cgcgcaggat gtccaggcgc tcgccggtga cctcaccgac
     7261 gatgcggatg cccaggccgg ggccggggaa cggctggcgc cagacgatcg cctccggaac
     7321 ccccagttcc agcccgacct gacggacctc gtccttgaac agcagccgca gcggctcgac
     7381 gagcttgaac tgcaggtcgt caggcaggcc gccgacgttg tggtgcgact tgatgttggc
     7441 cgtgcccgtg cccccaccgg actccacgac gtcgggatac agggtgccct gcacgaggaa
     7501 ctcgacgggg tggtcgtccg agcccgcgtg cgagccgacg acgtcgcgtg cggcctgctc
     7561 gaagacccgg atgaactcgc ggccgatgat cttgcgcttc tcctcggggt cggacacccc
     7621 ggccagggcc gtgaggaagc gctccttggc gtcgacgacg acgaggtcga cgcccgtggc
     7681 cgcgacgaag tcctgctcga cctgctcggc ctcacccgag cgcagcagcc cgtggtcgac
     7741 gaacacgcag gtcagctggt tgccgacggc acgctgcacg agggctgcgg cgaccgagga
     7801 gtcgacccca ccggacaggc cgcagatgac gcgcgcgtca ccgatctgct cccgcaccga
     7861 cgacacgagg tcatcgacga cgttggcggc cgtccactca cccgtgagac cggcgccccg
     7921 cagcaggaag ttctcgagca cctgctggcc gaacgtcgag tgcagcacct cggggtgcca
     7981 ctgcaccccg tagaggtgac gctcgtcgtc ctcgaagccc gcgacggggg ctcctgaggt
     8041 cgtggccgtc accgtcatcc cctccggggc ggccgagacg gagtcgccgt ggctcatcca
     8101 caccgactgc tcgacgggct ggccgttgaa cagtgtggac gtgctgtcga ggatggtggc
     8161 gggggtggcg ccgaactcgg agagcccggt gcgctccacc gtgccgccga gggcctgagc
     8221 catcgcctgg aagccgtagc acatgccgaa gaccggcacc ccggcctgta gcagcgccgg
     8281 gtccaggctc ggtgcgccgt cctcgtagac cgacgacggc ccgccggaga ggatcacggc
     8341 ggcagggtcc ttggccagca tctcggccac cggcatcgag tgcggcacga cctcgctgaa
     8401 gcagttggcc tcacgcaccc ggcgggcgat gagctgggcg tactgcgccc cgaagtcgac
     8461 gacgagcacc ggccggtcgg cgaggtgggt gctctcgggc tgcgtgctgg gggactcgct
     8521 cacccgcaca ggttactggc gcgccgccgc cagctcggac tcgacctgtc gggcaacacg
     8581 gcgctcgacc cagaacgaca ggaagggcac acagccggcg agcatgacga gcaccatctt
     8641 cgtcagcgac cagccgatct tgaacccgag cagggccgtg gcgacgaggt agaccatgta
     8701 gatgaagccg tgcggctggg gccaccagta cagcgggttg tcctcaccct tgagcccgaa
     8761 gccgtacgac agcaccatct cgacgacgag cacgagcagc ccgacgccga cgatgaaggc
     8821 catgaccttg aagaaggtca gccgcgagcg ggccccctcg acgtcgtcga tggtgtgggt
     8881 gcgggagtca ggcgtcgtca cgggacccag tatctcccgg cgcctcgagc agagcttcac
     8941 ggcgggtctc ctcgcgcacc atccggaacc acacccagag ggcgaacaac gcgaagaccc
     9001 accactgcac ggcgtacgcg gcgttgcgcc actggatgct cgtgtccggc agcggcgtcg
     9061 gcaccctggt cggctggccg gcagcctgcg gggtctcgga ctcgaggaag acgaaggcgt
     9121 tgtacagctc ccccggccag gtgttgacga gcaacgacag gtcgatcgac ccgatctcgc
     9181 cagccgccac cggcacggag gacggcgact cccccggcgc caacccaccc gcgatggtga
     9241 gggggcccga ggggggcgcc ccggcatccg ccgggttggt cacgaatccc cgcaccaccg
     9301 cgatcgtggc accggacccc tcgacgacga agggtgtgag cacccacgac ccgctcaccc
     9361 cgtcgagccg gcggtcgggc acgagcagct gaccgtcggg ggcgtagctg ccctgcgccg
     9421 tcaccgggcg tgaggacagg tcgccgcgaa acggcgcgtg cggctccagc accgaggtca
     9481 gcgccgccgg ctcctgggca cgggcctgct cgacggcctc acggtacgag cggctctcgg
     9541 cgaccccgag ctgccagcgg ccgaggaagg cgaacgcgat gcccacgacc accacgagac
     9601 cgaggaggcc gagaaaccga ggccgaaggg cggtccgcaa cacgccatcg aggctacgtc
     9661 accgcggaaa ctcgctggcc cgaggggggc tggcgcacct agcgtgggcg ggcgatgtct
     9721 cctcacccct ggccctacgc gaccgccccc gacgcggccc cgccgctgga cgccgaccgc
     9781 ctcatcctgt ggctggcgcg gcaccagtgg cgcaccctgc tcggcggggt gctcttcggc
     9841 gtgccctgga tgctgtcgat cgccctggtg ccggcggcga tcggcaaggc cgtcgacgac
     9901 ggtctggtcg cccgcgaccc gcgtgcgctc gtgacgtggg ctgccgcgat catggggctc
     9961 gggctggtga gcgcggtgac gacgaacggc cggcactggt tcgccgtgcg caactggctg
    10021 atctcgtcgt tccgcacggc ggtcgtcacc gagggcgcgg tgcgcggggc cgggccggcc
    10081 gtcacccggc agatgccggc cggtgaggtc gtcacgagct tcgcgagcga cttctggcgg
    10141 atgggcgagg tcttcgacgt caccgcccgc ctggccggcg ccatcgtcag cttcatcgtc
    10201 gtctcggcga tcctgctgcg cggatcggtg ctgctcgggg tcatcatgct cgtcggcggg
    10261 ccgctgctgc tggcctcact cgccctcgtc gtgcgaccgc tgcaccggcg ccagcaggcc
    10321 cagcgggagg aggccgggca gctcacggcg ctcggtgccg acaccgtcgc cgggctgcgg
    10381 gtgctgcgcg gcatcggcgg tgaggacacc ttcctcgccc ggtacgcggc gcagtcggag
    10441 cgggtgcgcc tcgccggagt tcgcctctcg ccggtgcagg cgacgttgga tgccgcccag
    10501 gtgctgttgc ccgggatctt cgtcgtcgtg gtcaccggca tcggcgccca cctggccgtc
    10561 ggcggtgaga tcacccccgg tcagctggtg gccttctacg ggtacacggc cttcctgacg
    10621 atgccgctgc gcacggcgac ggagttcgtc gacaagctga cccgcacccg ggtcgccgcc
    10681 cgccggatcg tgaagatcct cgcgatcgag cccgaccacg cgccggccgg gcgcacctcc
    10741 ggtgacctgt tggtcgatgg ggggacgccg gtggtggacg tgagcgcgga cctggtcgac
    10801 ccggcatccg gcgtcgtcgt cgtgcccggg cagttcaccg ccctggtgag cgccgacccc
    10861 gaggacaccg cgcagatcgc ccagcgcctc ggccggctgc tgcccgggcg ccacggcgtg
    10921 cgctggggcg aggtcgacct cgacgacctg ccgatcgacc gggtccgctc gctggtcgtc
    10981 gtctccggcg ccgaccccca cctgttcagc gggccggtgc gcgacgccct cggcggcgcc
    11041 gacgacgctg cgcgcgaggt cgcggtgcac gtcgcgagcg cctcggatgc catcgactcg
    11101 ctcgacggcg gtctggacgg cgagctcgag gagcgcggcc gtgccctgtc gggcgggcag
    11161 cgccaacggc tcgccctggc gcgggcggtg cttcgtgacc ccgaggtgct cgtgctcgtc
    11221 gaaccgacca gtgccgtcga cgcccacacc gaggcccgca tcgctcagcg acttgccgcg
    11281 cgccgtgcgg gacgcaccac tgtcgtcgtc acggcgagcc cgctcctgct cgacaccgct
    11341 gacaccgtgc tgttcgtgca ggacggggcg gccaccgcca ccgggcgcca ccgtgacctc
    11401 ctgcgcacgc atccggccta ccgcgacgtc gtgctgcgag gtgaggactg atgtcaccgc
    11461 tcctcggccg caccgaccac gcggcacgca ccctgccgat cgcgaccacc gcgacggtcc
    11521 gagcccacag caaggagctg ctgcgccacc accgcggccc gctgacctgg gtcctgctct
    11581 ggcacggcat cgccgccctc gtggccctgg tcggcccgtg ggtggtcggc caggtggtgc
    11641 aggaggtcac gaccgggtcg ccgagcctgc gccgggtcga cgtcctcatc gggctgctcg
    11701 tcgccgcgat cctcacccag acggtgctca cctgggtggc cagacgcgcc tcgttcgtgt
    11761 tgtccgagcg catcttcgcc cgcctgcgcg aggacttcat cacctcgtcg gtgcgccttc
    11821 ccctgtcgac ggtcgagcgc gccgggaccg gcgacctcgt ggcgcgcacg accaacgaca
    11881 tcgactcgtt ggcgcacatc atccgcttcg gcatcccggc catcgtcgtg gcggtgacga
    11941 ccgtgacggc caccgcggtc gcggccctgc tcacctcccc gctcgccgcg ctcgcgctgc
    12001 tgacggcggt tgtcgtgcac gtgccgacgt cacgctggta tctgcgccgc gccggcgacg
    12061 gctacctgtg ggaacacgcc tcctacgccc ggctcaacgg cgtcgcgtcg gagacgatcg
    12121 agggtgccgg cacgatggat gccctcggcc tccagaccgc ccgcggtcgt cgtttccagg
    12181 aggcgatgga cgagtgtggc gacgccgagg cctacacgct cggactgcga ctgcgctggt
    12241 tcccgtggct ggagatcggc ttcttcctgc ccatcgccac ctcggtgctc tggggtgggt
    12301 ggctggcctg ggagggtcac atctccatcg cggcagccac ggccgtgacg ctctacgtgc
    12361 agcagctgct cgacccgctc ggcgagctca tctcctggct cgacgagatc cagttcgcgg
    12421 cgacgtcact ggcccgcatc ctcggggtgg ccgaggtgcc gcccgaccgg gtcgcctccg
    12481 gggcccaacc cgtcggtgag cagatggagg tcgacgacgt ccgctactcc taccgccagg
    12541 gccgcgacgt gctgcacggc gtctcactgc gtctcgagcc cggcgaacgg ctggccatcg
    12601 tcggcccgtc gggggcgggc aagtcgaccc tcgggcggct gctcgccggc atcgacgggc
    12661 cgcgcaccgg ccgggtcacc ggtggcggcg ttcccctggt cgacctcgag ctgccggtgc
    12721 tgcgcggcga ggtggccctc gtgacccagg agcaccacgt cttcgtcggc accgtcgccg
    12781 agaacctgcg gctgccccgc ccggaggcaa ccgacgacga gcttcacgag gcgctggccg
    12841 cggtggatgc cgcggggtgg gccgccgccc tcccccacgg tctggacacc gtcgtcggct
    12901 ccggtgggca cccgctcacc gaggcccagg ggcagcagct cgcgctggcg cggctcgtgc
    12961 tggccgaccc gcacaccctc gtgctcgacg aggcgacgtc gctgctcgac ccgcgcgcgg
    13021 cccgccacct cgagcggtcg ctcgcggcgg tcgtcgacgg tcgcacggtg gtcgccatcg
    13081 cccaccggtt gcacaccgcc cacgacgccg accgggtcgc ggtcgtcgag gacggcgtca
    13141 tcagcgagct cggctcgcac gacgagctca tcgcgcggga cggctcctac gcggcgctgt
    13201 ggcgttcctg gcgcgacgag gcctgaccag gcaacgaccc ggtcagcgcg aggtgagctc
    13261 caactcaggc tcagcgggct tgctcgaact gtcgctgtgc accacatcgg aggactctcc
    13321 gtccacggct cgtcggcggc gcacgacgag ccacaccagc ccagccgcga gggccacgac
    13381 gacacccgtg ctcaccggcg cgatccactg ctcgatgacc gcgtagttct caccgagctg
    13441 gtagccggca gcgatgaaca gcgcgttcca gatcccgctc cccgccgcgg tcagcgcagc
    13501 gaaccgcggc gccggcatcc gctcgatccc tgcgggcacc gagaccaccg accgaacgag
    13561 cgggatgaag cgcccgaaga acaccacggc acggccgtgc ttggcgaaga accggtgccc
    13621 gcggttgagg tcagccgtgc tcagcaggaa gaaccagcgg cgacgcgaca ggtcgagcag
    13681 ccggtcgtac ccgagcgccc gccccaggcc gtagaggatg aacgcaccga cgacacttcc
    13741 cacggtcgcg gccgcccacg cgagcaccgg gtcgagccga cccgtcgcag ccgcgtagcc
    13801 cgcgaacggc aggatcacct cggaggggat cggcggcagc acggtctcga gcgccagcag
    13861 ggccccgacg ccgagttcgc caacggactc gatgaggtcg atgaccggtt cggtgacagc
    13921 ctgcatacga cacaacggta cgtaggtctc aaacggcccg ccaaatcccg agacacgaca
    13981 cgccgtcgga tgccgtcagc ggcccacgag ccggatcgtc acctcgaacg ggtcgtcgtc
    14041 gccgaccccc tcggccctgc ggaccgcctt cttcaccggg aggatgtagg ccccctgctc
    14101 ggtcgacggg aacagcgagg tgagccaggt gctcgacccc acggtgacct cgacgcgcac
    14161 cgacccgaag ccccccgtgg cgtccccgac gagctcgtcg ac
//
LOCUS       DAOSOC010000047        13793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4466_length_13793_cov_8.423788, whole genome shotgun sequence.
ACCESSION   DAOSOC010000047 DAOSOC010000000
VERSION     DAOSOC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4466_length_13793_cov_8.423788"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2247)
                     /gene="metH"
                     /locus_tag="PL014_05480"
     CDS             complement(<1..>2247)
                     /gene="metH"
                     /locus_tag="PL014_05480"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOT56576.1"
                     /translation="NAKAAIYALETLFEETDRRWPVMISGTITDASGRTLSGQVTEAF
                     WYSVRHARPLAIGLNCALGAGELRPYVAELSRIADCFVSAHPNAGLPNAFGEYDETPE
                     AMARVVGEFAASGLVNIVGGCCGTTPDHIAAIADAASQGVPRPPADVPPALRLAGLEP
                     CVIDEDSLFVNVGERTNITGSARFRRLITEEDYPTALGVARQQVEAGAQVIDVNMDEG
                     MIDGVAAMDRFLKLIATEPDICRVPLMIDSSKWEVIEAGLKVTQGKPIINSISMKEGE
                     AAFLDQARLARKYGAAVVVMAFDEQGQADNLERRREICGRAYRLLVDEVGFPAEDIII
                     DPNIFAVATGIEEHAAYGIDFISATAWIKENLPHALVSGGVSNVSFSFRGNNVVREAI
                     HAVFLFHAIKAGMDMGIVNAGALQVYDTVDERLRDAIEDVVLNRRPDAADRLLEIAEE
                     YRGSGEVADAAAEQWRSLPVRERITHALVKGLDEFVVDDTEELRLEIAAAGGRPLEVI
                     EGPLMDGMNVVGDLFGDGKMFLPQVVKSARVMKKAVAHLIPYIEAEKDEAARLKSKGL
                     VVTATVKGDVHDIGKNIVGVVLQCNNYDVIDLGVMVPTQKILDTAKEVGADIIGLSGL
                     ITPSLDEMVRVAAEMERQGFEIPLLLGGATTSRAHTAVKVDGQYHGPVVWVKDASRSV
                     PVVSQLLSEDQRGAFLDGVTADFDAIRARHAAKAGERPLASLEQARANRPVLDWASYR
                     PMRPHLLAQ"
     assembly_gap    2248..2347
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2348..2634)
                     /locus_tag="PL014_05485"
     CDS             complement(<2348..2634)
                     /locus_tag="PL014_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOT56577.1"
                     /translation="MTAAHRPDATADLTAALRERVLVLDGAMGTMIQGYGLSEADYRG
                     DRFADHPDDQQGNNDLLALTQPDIITAIHRAYLEAGSDLLETNTFNAQRISL"
     gene            2706..3818
                     /locus_tag="PL014_05490"
     CDS             2706..3818
                     /locus_tag="PL014_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT56578.1"
                     /translation="MPDPQAPSGSIRLGRVAGVPVYLDRTWLILGAFIAWTGWQAGRD
                     LGTTTGIAYAGWLVLGILTAVLGHEVAHAVAGRMLGFRVHRIVATLWGGHTSYDGTGS
                     TPGRAAAIAVSGPLANLALAGIGWIGLLVLTGPVAQFVLWWFVAINLLLAAFNLLPGL
                     PLDGGQVVHSLVWKVSGRRVTALLVAGWLGRLLAIAVVVFFAVLPVVQGVLDPFRLLL
                     VAVMAWVLWSGATAALRRAPFERLLDRLDAEEVIEPVGIVRADTPVGHLIGADRRLLA
                     IDDRGLPILLVPTASADVPDLTTLDPATPVSSLLVRLPDTSLIELAPGASVEPLLRAM
                     ALSGNGVVVVVSNGAVRGLVTADRLNAVAERHLRRN"
     gene            3830..4816
                     /locus_tag="PL014_05495"
     CDS             3830..4816
                     /locus_tag="PL014_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOT56579.1"
                     /translation="MTSPTGAELRRGPFTAGERVQLTDPKGRMHTIALAPGKQFHTHR
                     GYLAHDDLIGAPDGSTIKNTAGVEYLALRPLLADYVMSMPRGAAVIYPKDASQIVMMA
                     DMFPGAVVVEAGVGSGALSMSLLRAVGETGRVHSFERREDFAEIAKANAREYFGGDHP
                     AWTVTVGDLVEALPRTVENASVDRVVLDMLAPWECLEVVADALAPGGVLLCYVATATQ
                     LSKVAEAMRDADLFTEPQAWETLVRGWHLEGLAVRPQHRMHGHTGFLITTRRLAPGVT
                     PPLRKRRPGKGYVTGEEATEQDWTPEAIGERVPSDKRVRRVVRSVTDTSPLP"
     gene            4839..6590
                     /gene="arc"
                     /locus_tag="PL014_05500"
     CDS             4839..6590
                     /gene="arc"
                     /locus_tag="PL014_05500"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HOT56580.1"
                     /translation="MSTDEPTAEQAGTRESLEAQRESLEAEITALRAQGQASPAQLRT
                     LERDLAGLRSQLATMSAQNERLVRTLRDAREQIVTLKSEVDRLAQPPAAYGIVIETFE
                     DGTADILTSGRKMHVAVSPSLDADTLQAGREVMLNEAMNVVAVHGYETVGEIVLCKEV
                     LEDGRVLVVAQADEERVCRMAASLDGETVRAGDALLLESRSGFVFERIPKAEVADLVL
                     EEVPDIDYTDIGGLSRQIEAIRDAVELPYLHPDLYTEHQLKPPKGVLLYGPPGCGKTM
                     IAKAVAASLARKVAEKEGREPGKSFFLNIKGPELLNKYVGETERHIRLIFQRAREKSN
                     EGYPVVVFFDEMESLFRTRGSGVSSDVETTIVPQLLSEIDGVEGLEHVIVIGASNRED
                     MIDPAILRPGRLDVKIKIERPDAEAAREIFAKYLVETLPLHPDDVAQNGGSEQATVQA
                     MIDAVVLRMYTEEEENRFLEVTYAGGDKEILYFKDFNSGAMIQNIVDRAKKMAIKDFL
                     DTGQRGLRVEHLLQSCTDEFRENEDLPNTTNPDDWARISGKKGERIVYIRTLIKTKEG
                     TEPGKSINTVANTGQYL"
     gene            complement(6633..6980)
                     /locus_tag="PL014_05505"
     CDS             complement(6633..6980)
                     /locus_tag="PL014_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HOT56581.1"
                     /translation="MSRALPAVLDVALVVVFAALGRRSHAEGLDALGVLRTAAPFVAG
                     TAAGWLVASLTMDAGPRDLAFGAVVLVCTVVVGMLLRRVIGDGTAASFVIVATTVLSL
                     MLLGWRLMARLVA"
     gene            complement(6977..7969)
                     /locus_tag="PL014_05510"
     CDS             complement(6977..7969)
                     /locus_tag="PL014_05510"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOT56582.1"
                     /translation="MREVVVFSGSAHEPLAHRICDELGVELSQTALSRFSNDCLQAQL
                     LANCRQRDVYIVQPLVPPTQDSLMELLLMIDAAKGASAGMITAVIPHYAYARSDKKDA
                     SRISLGGRLVADLISTAGADRVLTMNLHAPQVHGFFSVPVDHLTALGVLADHFREQDL
                     DNTVVVSPDLGNAKDATQFSRLLGLPVAAGSKRRLGDDTVVIDSIVGDVEGKRAIVLD
                     DEIATGGSIIELLDRLADHGVTEAAVATTHGLFAGRAVERLRGHAMITEVVTTDTVPA
                     PKGWPELRVRSVAGLFAEAIKRIHEGESVSSLFDGVDPTHAPPRQSTLFDRVGS"
     gene            complement(7986..8969)
                     /locus_tag="PL014_05515"
     CDS             complement(7986..8969)
                     /locus_tag="PL014_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT56583.1"
                     /translation="MRSYRGVSPLIAAVSFAATHRGPPIEALDHHGIHRARAAVRPTH
                     PLLSWVTGRPDPAVGITFGTAPARDDHEIPLRIYRPRALRDSRTDVPVIMWFHGGGWV
                     LGNVVDYDPICTFLAAQVGAVVVSVDYRMAPEHRAPVAVNDCVDATTWVGVHGDVLHA
                     DTSRMAVAGDSAGGNLAAVVAQVLRDHGDTRIRHQALIYPATDATMSSPSVQQNAHAP
                     ILTRRSMVTFRDHYLGPGHDRRDPLVSPLFGRLDGLPPALVQTADLDPLRDDGIRYAE
                     ALQAAGVSARLTNYLKVPHGFASMPGAAPAVGRQHRWELATEVRSALSASG"
     gene            8990..10519
                     /gene="dop"
                     /locus_tag="PL014_05520"
     CDS             8990..10519
                     /gene="dop"
                     /locus_tag="PL014_05520"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HOT56584.1"
                     /translation="MTVRRVMGIETEYGIVVPGDPSANPMVASGDVVTTYATSRGIRP
                     ARASWDYADEAPLRDARGFDMGRGIAHPSQLTDVEDPTLANVVLTNGARLYVDHAHPE
                     YSSPEVTTPRDAVTWDRAGELVMREAVARLATMPPGINLYKNNTDGKGSSYGTHENYL
                     MSRATPFANIVRHLTPFFVVRQVMCGSGRVGIGVDSRTAGYQLAQRSDFFEVEVGLET
                     TLKRPIINTRDEPHAVADRYRRLHVIIGDANHADVANLLKMGTTSLVLAMIEADAVGA
                     DLSVRRPVATLHAVSHDPTLKTTIELVDGRRMTALDLLWEYHDRAAAFVEHTSGGEPD
                     PDTVEVMHWWGTVLDRLSRDPMEARREVDWVAKLAVLEGYRGRDSLGWGDPRLKAVDL
                     QWSDVRADRGLAHRLAATGRLEVLVEEDAVRAAVTQAPTDTRAWFRGECLRRFPDDVV
                     AASWDSVIFDVRGQRTLQRVPMLEPLRGTEAGVGDLVRNAPDAAALLAALARSSQGSG
                     D"
     gene            10556..10747
                     /locus_tag="PL014_05525"
     CDS             10556..10747
                     /locus_tag="PL014_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043806102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HOT56585.1"
                     /translation="MAGQEQIKPQRRDDGGPDDAPEAASAAPVISTSEIDDILDEIDG
                     VLESNAEEFVRGFVQKGGQ"
     gene            10744..11571
                     /gene="prcB"
                     /locus_tag="PL014_05530"
     CDS             10744..11571
                     /gene="prcB"
                     /locus_tag="PL014_05530"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HOT56586.1"
                     /translation="MTRDADGGRLPTAYLVPGSSSFTEFLSAHAPSLLPSGRGLPAGA
                     AIDAPHGTTIVSLTYDGGVVMAGDRRATMGNLIANRDMDKVFATDEFSLVGIAGTAGL
                     AIELVKLFQVELEHYEKIEGALMSLEGKANRLASMIRGNLGMAMQGLAVVPLFAGFDP
                     AAGTGRIFSYDVTGGCYEEHDHHSVGSGSLFARGALKKLYRRSGTVDDAVRCAVEALY
                     DAADDDSATGGPDVGRRIWPSVGIVDDSGARFLLDDEIAPVVDAIIAARVNNPGGAP"
     gene            11568..12374
                     /gene="prcA"
                     /locus_tag="PL014_05535"
     CDS             11568..12374
                     /gene="prcA"
                     /locus_tag="PL014_05535"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HOT56587.1"
                     /translation="MTAGMPFYVSPEQLMKDRADFARKGIARGRSVIVLAYDNGIAFV
                     AENPSRALHKVSEIYDRIAFAAVGKYNEFENLRVAGVRYADLRGYSYDRSDVTARGLA
                     NAYAQTLGTVFTQESKPYEVEIVVAEVGTSPDTDQVYRLTYDGSVADEHGFVAMGGGA
                     EQIETGLREQWRAGLHLSEALTLAVGLLGTDAAGGPARELSPSQLEVAVLDRHRPRRT
                     FRRITGPLLERLLSSEDPTRDVPAADDASSVRDASTNPQTQRPEQDGTPT"
     gene            12371..13750
                     /gene="pafA"
                     /locus_tag="PL014_05540"
     CDS             12371..13750
                     /gene="pafA"
                     /locus_tag="PL014_05540"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HOT56588.1"
                     /translation="MSTPGGRRIFGIENEYGITCSAGGQRTLTPDEVARYLFRKVVAW
                     GRSSNVFLTNGSRLYLDVGSHPEYATAECDSVRQVVAHDKAGERIVEGLVVDAQQRLR
                     EDGVVGDIYVFKNNTDSAGNSYGCHENYLVRRAGEFSSISDTLLPFLISRQITCGAGK
                     LVATSKGATYAVSQRADHIWEGVSSATTRSRPIINTRDEPHADAEHYRRLHVIVGDSN
                     MSETTTMLKVGSADLVLRMIEAGVVQRDLTLENPIRAIREISHDRTGRRLVRLANGRQ
                     MSALELQGEYYAKACEFIEREGATDPLHERVLELWGRTLRAVESDDLSLVDTEIDWVI
                     KYRLLDDYATRNGLPLEHPRLAQIDLAYHDIDRSRGLFYLLQRRGRAARVVSDPEVFS
                     AKVNPPPTTRAKLRGDFIRAAQANRRDFTVDWVHLKLNDQAQRTVLCKDPFLAQDARV
                     ERLIEGMTA"
BASE COUNT         2086 a         4900 c         4740 g         1967 t
ORIGIN      
        1 ctgcgccagc aggtggggcc gcatcggacg gtaggaggcc cagtcgagca cgggacggtt
       61 cgcccgcgcc tgctccagcg aggccagtgg ccgctcgccg gccttggcgg cgtgccgggc
      121 ccggattgcg tcgaagtcgg ccgtcacacc gtcgaggaag gcaccccgct ggtcctcgga
      181 cagcagctgg ctgaccacgg gcaccgaccg cgacgcgtcc ttcacccaga ccaccggccc
      241 gtggtactgc ccgtcgacct tgaccgccgt gtgggcccgg gaggtcgtcg ccccgccgag
      301 cagcagcggg atctcgaagc cctggcgctc catctcggcc gcgacccgca ccatctcgtc
      361 caggctcggg gtgatcagac cggacaaccc gatgatgtcg gccccgacct ccttcgcggt
      421 gtcgaggatc ttctgggtcg gcaccatgac cccgaggtcg atgacgtcgt agttgttgca
      481 ctgcagcacc acacccacga tgttcttgcc gatgtcgtgc acgtcgccct tgaccgtggc
      541 cgtgacgacc aggcccttgc tcttcaggcg ggcggcctcg tccttctccg cctcgatgta
      601 cgggatgagg tgggccaccg ccttcttcat gacccgcgcc gacttcacga cctgcggcag
      661 gaacatcttg ccgtcgccga agaggtcacc gacgacgttc atgccgtcca tgagcggccc
      721 ctcgatcacc tcgagcggac gcccacccgc agcggcgatc tcgaggcgca actcctcggt
      781 gtcgtcgacg acgaactcgt cgagcccctt gaccagcgcg tgcgtgatcc gttcgcgcac
      841 cggcagcgag cgccactgct cggccgccgc atcggccacc tccccgctgc cgcggtactc
      901 ctcggcgatc tcgagaagcc ggtccgcggc atccggacgg cggttgagca cgacgtcctc
      961 gatcgcgtcg cgcaacctct cgtcgacggt gtcgtacacc tggagggccc cggcgttgac
     1021 gatccccatg tccatcccgg ccttgatggc gtggaagagg aacacggcgt ggatggcctc
     1081 gcgcacgacg ttgttgccgc ggaagctgaa cgagacgttg gagacaccgc cggacaccag
     1141 ggcgtgcggc aggttctcct tgatccacgc cgtggccgag atgaagtcga tgccgtaggc
     1201 cgcgtgctcc tcgatgcccg tggccaccgc gaagatgttc gggtcgatga tgatgtcctc
     1261 ggccgggaag ccgacctcgt cgacgagcaa ccggtaggcg cggccacaga tctcgcggcg
     1321 ccgctcgagg ttgtcggcct gcccctgctc gtcgaacgcc atgacgacca ccgcggcgcc
     1381 gtacttgcgc gcgagcctgg cctggtcgag gaacgccgcc tcgccctcct tcatggagat
     1441 cgagttgatg atcggcttgc cctgggtgac cttgagcccg gcctcgatga cctcccactt
     1501 gctggagtcg atcatcagcg gcacccggca gatgtcgggc tcggtcgcga tgagcttgag
     1561 gaagcggtcc atcgccgcga ccccgtcgat catcccctcg tccatgttga cgtcgatgac
     1621 ctgggccccg gcctccacct gctggcgggc gaccccgaga gcggtggggt agtcctcctc
     1681 ggtgatcagc cgccggaacc gcgccgaccc cgtgatgttg gtgcgctcgc cgacgttgac
     1741 gaacaggctg tcctcgtcga tgacgcacgg ttcgagcccg gccaggcgca gggcgggcgg
     1801 cacgtccgcg ggcgggcgcg gtacgccctg ggatgccgcg tcggcgatcg ccgcgatgtg
     1861 gtccggtgtg gtgccgcagc acccaccgac gatgttgacc agtccggatg ccgcgaactc
     1921 accgacgacg cgcgccatcg cctcgggcgt ctcgtcgtac tccccgaagg cgttgggcag
     1981 cccggcgttg gggtgcgcgg agacgaagca gtcggcgatc cgggagagtt cggcgacgta
     2041 ggggcgcagt tcaccggcgc cgagggcaca gttgaggccg atggccagcg ggcgggcatg
     2101 gcgcaccgag taccagaagg cctcggtgac ctgacccgag agggtgcgcc cggacgcgtc
     2161 ggtgatggtc cccgagatca tcaccggcca gcgccggtcg gtctcctcga acagggtctc
     2221 gagggcgtag atcgccgcct tggcgttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnagc gagatccgct gcgcgttgaa ggtgttggtc tcgagcaggt ccgagcccgc
     2401 ctcgaggtag gcccggtgga tggcggtgat gatgtcgggc tgggtgagcg ccaacaggtc
     2461 gttgttgccc tgctggtcgt ccgggtggtc ggcgaaccgg tcgccccggt agtcggcctc
     2521 ggacagccca tacccctgga tcatcgtgcc catcgccccg tcgagcacga ggacccgctc
     2581 gcgcagggcg gcggtgaggt cggcggtggc gtccgggcgg tgggcagcag tcacgtcgag
     2641 tcgtctcagt cagggttgaa cgggcaagga caagtatggc gcgagcctca cgtagggtcc
     2701 gtggcatgcc cgacccgcaa gcaccatccg gctcgatccg gctcggccgg gttgccgggg
     2761 tgccggtcta cctcgaccgc acctggctga tcctcggcgc cttcatcgcg tggaccggct
     2821 ggcaggcggg ccgcgacctc gggacgacca cggggatcgc gtacgccggg tggctcgtcc
     2881 tcggcatcct gaccgcggtg ctggggcacg aggtcgccca cgccgtggcc ggccggatgc
     2941 tgggtttccg ggtgcaccgc atcgtcgcca ccctgtgggg tggtcacacg tcgtacgacg
     3001 gcaccggctc gaccccgggg agggctgccg cgatcgcggt gagcggcccg ctggccaacc
     3061 tcgccctggc ggggatcggg tggatcggtc ttctggtgct gaccgggccg gtggcgcagt
     3121 tcgtgctctg gtggttcgtc gcgatcaacc tcctgctcgc cgccttcaac ctcctgcccg
     3181 gccttccgct ggacggtggg caggtcgtgc actccctggt gtggaaggtc agcggacgcc
     3241 gggtcaccgc gctgctcgtc gccggctggc tgggccgcct cctcgccatc gcggtggtcg
     3301 tgttcttcgc agtcctgccc gtggtgcagg gcgtcctcga cccgttccgg ctgctcctcg
     3361 tcgccgtcat ggcctgggtc ctgtggtccg gtgccacggc ggcgctgcgc cgagccccct
     3421 tcgagcggct gctcgaccgg ctcgacgccg aggaggtcat cgagccggtc gggatcgtgc
     3481 gcgccgacac gcccgtcggc cacctgatcg gggctgaccg ccgtctgctc gccatcgacg
     3541 accgcgggct gccgatcctg ctggtgccga cggcatccgc cgacgtcccc gacctcacca
     3601 ccctcgaccc ggccaccccg gtgtcctccc tcctcgtgcg gctgcccgac acctcactca
     3661 tcgagctcgc ccccggtgcc agcgtcgagc cgctgctgcg tgcgatggcg ctgtccggca
     3721 acggggtggt cgtcgtcgtc tcgaacggtg cggtgcgggg cctcgtcacc gctgaccgcc
     3781 tcaacgccgt ggccgaacgc cacctgcgtc gcaactagac tccccgccca tgacctcacc
     3841 gaccggcgcc gagctgcggc gtggcccctt caccgcgggc gagcgcgtcc agctgaccga
     3901 ccccaagggc cgcatgcaca ccatcgccct ggccccgggc aagcagttcc acacccaccg
     3961 ggggtacctc gcccacgacg acctgatcgg tgcccctgac ggctcgacga tcaagaacac
     4021 cgccggcgtc gagtacctcg cgctgcgccc gctgctcgcc gactacgtga tgtcgatgcc
     4081 ccggggcgcc gcggtgatct atcccaagga cgccagccag atcgtcatga tggccgacat
     4141 gttcccgggt gcggtcgtcg tcgaggccgg ggtcggctcc ggagcgctgt cgatgtcgct
     4201 gctgcgggcc gtcggcgaga ccggtcgggt gcactcgttc gagcgtcggg aggacttcgc
     4261 cgagatcgcc aaggccaacg cccgcgagta cttcggcggt gaccaccccg cgtggaccgt
     4321 caccgtcggt gacctcgtcg aggcgctgcc gcgcacggtg gagaacgcaa gcgtcgaccg
     4381 ggtcgtgctc gacatgctgg cgccgtggga gtgcctcgag gtcgtcgccg acgcgctcgc
     4441 cccgggcgga gtgctgctct gctacgtggc gacagccacc cagttgtcca aggttgccga
     4501 ggccatgcgc gacgccgacc tgttcaccga gccacaggct tgggagacgc tggtgcgcgg
     4561 ctggcacctc gaaggcctgg ccgtccgtcc gcaacaccgg atgcacgggc acaccgggtt
     4621 cctcatcacc acccgccgcc tcgcaccggg ggtcacgccc ccgctgcgca agcgccgccc
     4681 gggcaagggg tacgtcacgg gcgaggaggc gaccgagcag gactggaccc ccgaggccat
     4741 cggcgagcgg gtcccctcgg acaagcgcgt gcgccgcgtc gtgcgttcgg tgaccgacac
     4801 ctcaccgctt ccgtgacgca cgcgggttat cgtcgaccat gagcaccgac gagcccacgg
     4861 ccgagcaggc gggcacccgc gagagcctcg aggcccagcg ggagtcactc gaggccgaga
     4921 tcaccgccct gcgcgcccag gggcaggcca gtcccgccca gctgcgcacc ctcgaacgtg
     4981 atctcgccgg gctgcggtcc cagctcgcga cgatgtccgc gcagaacgaa cgactggtgc
     5041 gcacgcttcg ggacgcccgg gagcagatcg tcacgctgaa gtccgaggtc gaccggctcg
     5101 cccagccccc cgccgcctac ggcatcgtca tcgagacctt cgaggacggc accgccgaca
     5161 tcctgacgag cgggcgcaag atgcacgtcg ccgtcagccc ttcgctcgac gcggacaccc
     5221 tccaggccgg ccgcgaggtc atgctcaacg aggcgatgaa cgtcgtcgcg gtgcacgggt
     5281 acgagaccgt gggcgagatc gtgctctgca aggaggtcct ggaggacggt cgagtgctcg
     5341 tcgtcgccca ggccgacgag gagcgtgtct gccggatggc ggcctcgctc gacggtgaga
     5401 ccgtgcgcgc cggtgacgcc ctgctgctgg agtcgcgctc ggggttcgtc ttcgagcgga
     5461 tccccaaggc cgaggtcgcc gacctcgtgc tcgaggaggt ccccgacatc gactacaccg
     5521 acatcggtgg gctcagcagg cagatcgagg ccatccggga tgccgtcgag ctgccctacc
     5581 tgcaccccga cctctacacc gagcaccagc tcaagccccc gaagggggtg ctgctctacg
     5641 gcccgcccgg ctgtgggaag acgatgatcg ccaaggcggt cgcggcctcg ctggcccgca
     5701 aggtcgccga gaaggagggc cgcgaacccg gcaagtcctt cttcctcaac atcaagggcc
     5761 ccgagctgct caacaagtac gtcggcgaga ccgagcgcca catccgcctc atcttccagc
     5821 gggcccgcga gaagtccaac gagggctacc cggtcgtcgt gttcttcgac gagatggagt
     5881 cgctgttccg cacccgcggg tcgggtgtgt cctccgacgt cgagacgacg atcgtgccgc
     5941 agctgctctc cgagatcgac ggcgtcgaag ggctcgagca cgtcatcgtg atcggtgcct
     6001 cgaaccgcga ggacatgatc gacccggcca tcctgcgacc gggacgcctg gacgtgaaga
     6061 tcaagatcga gcgccccgac gccgaggctg cccgtgagat cttcgcgaag tacctcgtcg
     6121 agaccctgcc gctgcacccg gacgacgtcg cccagaacgg cggctcggag caggcgaccg
     6181 tgcaggccat gatcgatgcc gtggtgctgc gcatgtacac cgaggaggag gagaaccgct
     6241 tcctcgaggt gacctacgcc ggtggtgaca aggagatcct gtacttcaag gacttcaact
     6301 ccggcgcgat gatccagaac atcgtcgacc gcgccaagaa gatggccatc aaggacttcc
     6361 tcgacaccgg tcagcggggt ctgcgggtcg agcacctgct ccagtcgtgc accgacgagt
     6421 tccgcgagaa cgaggacctc ccgaacacga cgaaccccga cgactgggcg cgcatctccg
     6481 gcaagaaggg ggagcggatc gtctacatcc gcacgctgat caagaccaag gagggcaccg
     6541 agcccggcaa gtcgatcaac acggtcgcca acaccggcca gtacctgtga ttcgcggtgc
     6601 ctgaccgctg acccccgggg tcgggccgca cgtcaggcga cgaggcgggc catcagtcgc
     6661 cagccgagga gcatcagcga cagcacggtg gtcgcgacga tcacgaacga ggcagcagtg
     6721 ccgtcgccga tgacccggcg cagcagcatt ccgacgacga cggtgcacac gagcaccacg
     6781 gcaccgaacg ccaggtcgcg cggccccgca tccatggtca ggctggcgac caaccagccg
     6841 gccgctgtcc cggcgacgaa gggcgcggcg gtgcgcagca ccccgagcgc gtcgagaccc
     6901 tcggcgtgcg agcgccggcc gagggcagcg aagacgacga cgagggcgac gtcgaggacg
     6961 gctggtaggg cccggctcac gagccgaccc ggtcgaacag cgtgctctgc cgcggcgggg
     7021 cgtgcgtggg gtcgacgccg tcgaacaggc tcgacaccga ctcgccctcg tggatgcgct
     7081 tgatggcctc ggcgaacagc ccggccaccg agcgcacccg gagctccggc cagcccttgg
     7141 gagcgggcac cgtgtcggtg gtgacgacct cggtgatcat cgcgtgaccg cgcagccgct
     7201 cgacggcccg gccggcgaac aggccgtggg tcgtcgcgac ggccgcctcg gtgacgccgt
     7261 ggtcggccaa ccggtcgagc agctcgatga tcgagccccc cgtggcgatc tcgtcatcga
     7321 gcacgatggc ccgcttgccc tcgacgtcgc cgacgatgga gtcgatgacg acggtgtcgt
     7381 cgccgagccg gcgcttggac ccggcagcga cgggcagccc gagcagccgc gagaactggg
     7441 tggcgtcctt ggcgttgccg aggtcggggg agacgacgac ggtgttgtcg aggtcctgct
     7501 cgcggaagtg gtcggcgagc acgccgagcg cggtgaggtg gtcgacgggc acggagaaga
     7561 agccgtgcac ctgcggggcg tgcaggttca tcgtgagcac gcggtcggca cccgcggtcg
     7621 agatcaggtc agccacgagc cggcccccga gcgagatgcg cgaggcgtcc ttcttgtcgc
     7681 tgcgggcgta ggcgtagtgg gggatgaccg cggtgatcat gcccgcggag gcgcccttgg
     7741 cggcatcgat catgagcagc agctccatga gcgagtcctg cgtcgggggc acgagcggct
     7801 ggacgatgta gacgtcgcgc tggcggcagt tcgccagcag ctgggcctgg aggcagtcgt
     7861 tgctgaaccg gctgagcgcc gtctgcgaca gctccacccc gagttcgtcg cagatgcggt
     7921 gggccagggg ctcgtgggcc gacccggaga agacgacgac ctcgcgcatg tccgcagcct
     7981 agtgctcagc ccgatgccga cagggcgctt cgcacctcgg ttgcgagctc ccaccggtgc
     8041 tgtcgcccca ccgccggtgc cgcacccggc atcgacgcga agccgtgcgg caccttgagg
     8101 tagttcgtca gccgggccga gacaccggct gcctgcagtg cctcggcgta ccggatgccg
     8161 tcgtcgcgca gcggatcgag gtcggccgtc tgcaccagtg cgggcgggag accgtccagc
     8221 cgaccgaaca gcggtgacac gagcgggtcg cgccggtcgt ggcccgggcc gaggtagtgg
     8281 tcgcgaaagg tcaccatcga ccgcctggtg aggatgggcg cgtgcgcgtt ctgctgcacc
     8341 gagggtgagc tcatcgtggc gtcggtcgca ggatagatca gggcctggtg tcgaatcctg
     8401 gtgtcgccgt ggtcgcgcag cacctgggcg accacggccg cgaggttgcc gcccgccgag
     8461 tcaccggcca cggccatgcg cgaggtgtcg gcgtgcagga cgtcaccgtg cacccccacc
     8521 caggtcgtgg cgtccacgca gtcgttcacc gccaccgggg cgcggtgctc gggcgccatc
     8581 cggtagtcga ccgagacgac gaccgccccc acctgcgccg cgaggaaggt gcagatcggg
     8641 tcgtagtcga cgacgttgcc gagcacccat ccgccgccgt ggaaccacat gatgaccggg
     8701 acgtcggtgc ggctgtcccg cagggctcgg ggtcggtaga tccgcagcgg gatctcgtgg
     8761 tcgtcgcggg caggtgccgt gccgaacgtg atgccgaccg ccgggtcagg gcgccccgtc
     8821 acccacgaca ggaggggatg ggtgggccgc accgcggcgc gcgcccggtg gatgccgtgg
     8881 tggtccagcg cctcgatggg tgggccacgg tgggtcgccg cgaacgagac cgcggcgatg
     8941 aggggggaga ctccgcggta gctgcgcacg cggtcagtat ggtcgagcca tgacggtgcg
     9001 cagggtgatg gggatcgaga ccgagtacgg aatcgtcgtc ccgggggatc cgagcgcaaa
     9061 tcccatggtc gcctcgggcg atgtcgtcac gacgtacgcg acctcacgcg gcatccgacc
     9121 ggcgagggcc agctgggact acgccgacga ggcgccgctg cgcgacgccc gcggtttcga
     9181 catgggccgc ggaatcgccc accccagcca gctgacggac gtggaggacc cgacgctggc
     9241 gaacgtcgtg ctcaccaacg gcgcccggct gtacgtcgac cacgcccacc ccgagtacag
     9301 ctcgcccgag gtgacgactc cccgggacgc cgtcacgtgg gaccgcgccg gtgagctcgt
     9361 catgcgcgag gccgtcgccc gcctggcgac gatgccgccc ggcatcaacc tctacaagaa
     9421 caacaccgac ggcaagggct cgtcctacgg cacgcacgag aactacctca tgtcacgggc
     9481 gaccccgttc gcgaacatcg tgcggcacct gacgccgttc ttcgtcgtcc ggcaggtcat
     9541 gtgcggttcg ggccgcgtcg gcatcggggt cgactcccgt accgccggct accagctggc
     9601 ccagcgcagc gacttcttcg aggtcgaggt cggcctggag acgaccctga agcgcccgat
     9661 catcaacacc cgcgacgagc cgcacgcggt ggccgaccgc taccgccgcc tgcacgtcat
     9721 catcggcgac gccaaccacg ccgacgtggc gaacctgctc aagatgggca cgacgtccct
     9781 cgtgctcgcc atgatcgagg cggatgccgt gggcgccgac ctctccgtgc gccgcccggt
     9841 ggccacgctg cacgccgtgt cccacgaccc gacgctgaag accacgatcg agctcgtcga
     9901 cggccgccgg atgaccgccc tcgacctgct gtgggagtac cacgaccgtg cggcggcctt
     9961 cgtcgagcac acctctggtg gcgagcccga cccggacacc gtcgaggtga tgcactggtg
    10021 gggcacggtg ctcgaccggc tctcgcgcga tcccatggag gcccgccgcg aggtcgactg
    10081 ggtcgccaag ctcgccgtgc tcgaggggta ccgcgggcgc gactccctcg ggtggggtga
    10141 cccgcggctc aaggccgtgg acctccagtg gtccgacgtg cgggcggacc gggggctggc
    10201 gcaccgactc gccgccaccg gccgtctcga ggtgctcgtc gaggaggatg ccgtgcgcgc
    10261 ggcggtcacg caggccccca ccgacacccg ggcgtggttc cggggtgagt gcctgcggcg
    10321 cttcccggac gacgtcgtgg cggcctcctg ggactcggtg atcttcgacg tccggggcca
    10381 gcgcaccctc cagcgggttc ccatgctcga accgctgcgc ggaaccgagg ccggggtggg
    10441 cgatctggtc cgcaacgctc cggacgccgc cgcgctgctg gcggccctgg cacgcagcag
    10501 ccagggctct ggcgactagg ctcgaaccag tctcacgcac acacgaggga gcaccatggc
    10561 cggccaggag cagatcaagc cacagcgtcg cgatgacggc ggccccgacg acgcccccga
    10621 ggcggcgagc gcggcacccg tcatcagcac gagcgagatc gacgacatcc tcgacgagat
    10681 cgacggggtg ctggagtcca acgccgagga gttcgtgcgc ggtttcgtcc agaagggcgg
    10741 ccagtgaccc gcgacgccga cggtggccgc ctccccacgg catacctcgt gcccgggtcg
    10801 tcgtcgttca ccgagttcct gtccgcccac gcaccgagcc tgctgccctc cgggcggggc
    10861 ctgcccgcag gtgccgcgat cgacgccccg cacgggacga cgatcgtctc gctgacctac
    10921 gacgggggcg tcgtcatggc cggtgaccga cgggccacga tgggcaacct catcgcgaac
    10981 cgcgacatgg acaaggtctt cgcgaccgac gagttcagtc tcgtcggcat cgccggcacc
    11041 gccggactgg ccatcgagct cgtcaagctg ttccaggtcg agctcgagca ctacgagaag
    11101 atcgagggcg ccctgatgtc cctcgagggc aaggccaacc ggctcgcgtc gatgatccgc
    11161 ggcaacctcg gcatggcgat gcaggggctg gccgtcgtgc cgctgttcgc cggcttcgac
    11221 cccgcagcgg gcacgggccg gatcttctcc tatgacgtga ccgggggctg ctacgaggag
    11281 cacgaccacc acagcgtcgg ctcgggctcg ctgttcgccc gcggggccct gaagaagctc
    11341 taccgccgct ccggcacggt cgacgacgcg gtgcgctgcg ccgtcgaggc cctctacgac
    11401 gccgccgacg acgactccgc caccggtggc ccggacgtcg gacgccgcat ctggccctcc
    11461 gtcggcatcg tcgacgactc cggcgcccgc ttcctgctcg acgacgagat cgccccggtc
    11521 gtcgacgcca tcatcgccgc gcgcgtcaac aacccgggag gtgccccatg accgccggga
    11581 tgccgttcta cgtctcgccc gagcagctca tgaaggaccg ggccgacttc gcgcgcaagg
    11641 gcatcgcgcg cggccgctcg gtcatcgtgc tcgcctacga caacggcatc gcgttcgtcg
    11701 ccgagaaccc gagccgggcg ctgcacaagg tctccgagat ctacgaccgg atcgccttcg
    11761 ccgccgtcgg caagtacaac gagttcgaga acctgcgggt ggccggggtg cggtacgccg
    11821 acctgcgggg gtactcctac gaccgctcgg acgtcaccgc ccgtgggctg gccaacgcct
    11881 acgcgcagac gctgggcacg gtgttcaccc aggagtccaa gccctacgag gtcgagatcg
    11941 tcgtcgccga ggtcggcacc agcccggaca ccgaccaggt gtaccgcctc acctacgacg
    12001 gttccgtcgc cgacgagcac gggttcgtcg ccatgggcgg cggcgcggag cagatcgaga
    12061 ccggcctgcg ggagcagtgg cgcgccgggc tgcacctgtc ggaggcgttg accctggccg
    12121 tcggcctgct cggcaccgac gccgcagggg gcccggcccg tgagctcagc ccgtcccagc
    12181 tcgaggtcgc cgtgctcgac cggcaccggc cgcggcgcac cttccgacgg atcaccggcc
    12241 cgctgctcga gcgcctgctg agcagcgagg accccacccg tgacgtcccc gccgccgatg
    12301 acgccagttc ggtgcgcgat gcgagcacca acccacagac ccagcgcccc gagcaggacg
    12361 gcacgcccac gtgagcaccc ccggcgggcg ccgcatcttc ggcatcgaga acgagtacgg
    12421 catcacctgc tcggccgggg gccagcgcac cctgacgccc gacgaggtgg cgcgctacct
    12481 cttccgcaag gtcgtcgcct ggggccggtc gagcaacgtc ttcctcacca acgggtcgcg
    12541 gctctacctc gacgtcggtt cccaccccga gtacgccacc gccgagtgcg actcggttcg
    12601 ccaggtcgtg gcccacgaca aggcggggga gcggatcgtc gaagggcttg tcgtcgacgc
    12661 ccagcagcgg ctgcgcgagg acggcgtcgt cggcgacatc tacgtcttca agaacaacac
    12721 cgactccgcg ggcaactcct acggctgcca cgagaactac ctcgtgcgcc gggccgggga
    12781 gttctcctcg atctccgaca ccctgctgcc gttcctcatc agccgccaga tcacctgtgg
    12841 tgcaggcaag ctcgttgcca cctccaaggg cgcgacctat gccgtcagcc agcgggccga
    12901 ccacatctgg gagggcgtgt cctcggccac gacacgcagc cgccccatca tcaacacccg
    12961 cgacgaaccg cacgccgacg ccgagcacta ccgccggctg cacgtcatcg tcggcgactc
    13021 caacatgagc gagaccacga cgatgctcaa ggtgggctcg gccgacctgg tgctgcggat
    13081 gatcgaggcg ggtgtcgtcc agcgtgacct caccctggag aaccccatcc gcgcgatccg
    13141 cgagatctcc cacgaccgca cggggcggcg gctggtccgc ctggccaacg gccgccagat
    13201 gagcgccctg gagctccagg gggagtacta cgccaaggcc tgcgagttca tcgagcgtga
    13261 gggcgcgacc gacccgctgc acgaacgagt cctcgagctg tgggggcgca cgctgcgggc
    13321 cgtggagagc gacgacctca gcctcgtcga caccgagatc gactgggtca tcaagtaccg
    13381 gttgctcgac gactacgcga cccgcaacgg actgcccctc gagcaccccc ggctggccca
    13441 gatcgacctc gcctaccacg acatcgatcg cagcaggggg ctgttctacc tgctccagcg
    13501 gcgcggccgg gccgcccggg tcgtcagcga ccccgaggtc ttctcggcca aggtcaaccc
    13561 gccgccgacg acccgggcca agctgcgcgg cgacttcatc cgggcggcgc aggccaaccg
    13621 tcgggacttc accgtcgact gggtgcacct caagctcaac gaccaggctc agcgcacggt
    13681 cttgtgcaag gacccgttcc tcgcgcagga cgcccgcgtc gagcgcctca tcgagggcat
    13741 gaccgcctga tcgacgcggc cggtgagcgg aggtgacgcg aacggcatcc agc
//
LOCUS       DAOSOC010000048        13652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4526_length_13652_cov_10.718247, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000048 DAOSOC010000000
VERSION     DAOSOC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_13652_cov_10.718247"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PL014_05545"
     CDS             complement(<1..311)
                     /locus_tag="PL014_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT56589.1"
                     /translation="MSTHPRQRRLHRIAMVTVHTSPLAQPGEGDAGGMNVYVLEVARQ
                     LARRDIAVDLFTRTTCAAQSPVVEVEPGVVVRHLAAGPYEGLSKEDLPGQLCAFAAGM
                     MR"
     gene            complement(326..1069)
                     /locus_tag="PL014_05550"
     CDS             complement(326..1069)
                     /locus_tag="PL014_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56590.1"
                     /translation="MSLSMLALVVSLSGALVLGLTSWVPRVMARDIGIVDVEPGLLEV
                     AQGARGVQSLGAGLSVSLFTSGFRLARGSEVLADTVTRGAPVSAMFGTVTEGGDHPRE
                     TVSGVLSTVRIDAVRRVGDVVEYLGVVRDSSTSRPLRIKFEPTDGSIRMDVLVPAVDA
                     LAIHLDWRPATTGIAPALPERNLREQAYWVDPTVIAQPAFTWVLGTDVSIGPVQVPRA
                     VDLRQDGRIAIHVWAPRAVVTVTDTPRMP"
     gene            1175..1975
                     /locus_tag="PL014_05555"
     CDS             1175..1975
                     /locus_tag="PL014_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT56591.1"
                     /translation="MTRQRPHGVITRGTTNPNRLRRCDRWLAGPQGWRLRAHPGPPVV
                     VDLGYGASPVTAVELHDRLSAVRPDVEVVGIEIDPSRVAAARPLERPGLRFAVGGFEV
                     PLPQGRRPVIVRAFNVLRQYDEGEVAAAWDATASRLAPDGLLVDGTCDELGRVAAWVA
                     LTASGPVSLTISLRLRDLERPGIVAERLPKALIHRNVPGERIHRLLTELDEAWARQAP
                     HAAYGVRQRFIATAGALRDAGWPVLDGPARWRVGELTLAWEAVAPRAL"
     gene            complement(2002..>2121)
                     /locus_tag="PL014_05560"
     CDS             complement(2002..>2121)
                     /locus_tag="PL014_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HOT56592.1"
                     /translation="GFLAIIAIVAAGVYLADVKPALDQVMGRGSGNQGPYGPW"
     assembly_gap    2122..2221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2222..2418)
                     /locus_tag="PL014_05565"
     CDS             complement(<2222..2418)
                     /locus_tag="PL014_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT56593.1"
                     /translation="MRAVLQRVSSARVVVAGEVVGELTRPGLVALVGVTHDDGPAQVD
                     TLARKIADLRILHGERSVLDEG"
     gene            complement(2426..3484)
                     /locus_tag="PL014_05570"
     CDS             complement(2426..3484)
                     /locus_tag="PL014_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOT56594.1"
                     /translation="MPQQTSSSAHPSGPPVSTVTPPALAEAPVLVHVTRGGVVESVHR
                     ASVAVTASDGSVLQAWGDPHDPVFPRSSNKPLQSVAMVRAGLDLPPHHLALASASHSA
                     EPFHLDAVRDMLAAAGLTEADLQNTADLPYDPDERDVWIAAGRAARSLAQNCSGKHAA
                     MLATCRVNGWDIATYRDPRHPLQQLMAQTIADLAGEPVAAMAVDGCGAPVMAISLAGL
                     ARAFGRVAAADPGTHEGRVADAIRQHPEYLGGTRRDVTALIQGTQGVIAKDGAEAVYA
                     VGLADGRGIALKIADGGQRARPVVLAAVLRRLGVESSAYERLESAPILGHGQPVGAVV
                     AADLDGTMSVGGSTTHAG"
     gene            complement(3663..4493)
                     /locus_tag="PL014_05575"
     CDS             complement(3663..4493)
                     /locus_tag="PL014_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56595.1"
                     /translation="MSVMTDIKNTVDATPFFAAVGATDLAVEKVREARIRAEKVRVEL
                     SADLAPAKVQARATTLVDGVKEIPAVVLNETMVVGGKVAEGYDELAVRGKNLVNRIRN
                     QKSTKDLFAQAETSVAQAKGAVTSARNAAADIERSAKATITTTRKEAVKLAEVVTGSV
                     TEGLETVEAEVTESAKRTRTAAKRTSTTTKNASKKAVSSAKAATTSARKTAVAAEKAT
                     EKAAEKVGDVPAAKRTPAKRTVKVSTKPAAKRTSAPAAKPAAKTADTPAASATVTVTA
                     "
     gene            complement(4547..4909)
                     /locus_tag="PL014_05580"
     CDS             complement(4547..4909)
                     /locus_tag="PL014_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT56596.1"
                     /translation="MSKNPLNDLGSSLGDYLREQRTAAKLSLRQLSELAGVSNPYLSQ
                     IERGLKRPSAEILQQLAKGLEVSAESLYVKAGILDAEVTPHSETPDVRGAIAADPALT
                     ERQRKTLLDIYESYVEDN"
     gene            complement(5015..5518)
                     /locus_tag="PL014_05585"
     CDS             complement(5015..5518)
                     /locus_tag="PL014_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOT56597.1"
                     /translation="MADVRTPFEFPAVVNEKAARAVAGLVVLVAAVALLTGWLWLSAV
                     LAVGFALRVASGPRFDPFGQLATKVIAPRLGEPVLVPGPPKRFAQAIGLVLTLVATGF
                     LVAGLPLVTTALLGILLVFATLESAFGFCAGCYAFGLLMRAGLVPEETCAACNDISLR
                     QRQPAGV"
     gene            complement(5622..6476)
                     /locus_tag="PL014_05590"
     CDS             complement(5622..6476)
                     /locus_tag="PL014_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOT56598.1"
                     /translation="MATSSTPVGTLITVAPTGAEHAKADLPQLPTTLDELVETARRCE
                     AAGAALIHLHIRDADHAPTLDRGLLKASVDAVRSETDLIVQLSTGGSVHDPLETRLTV
                     LDAEPDSCSLTCGTTNFGDDVFLNPWGFMSDLYVQAQEREVVPEFELFDLGHVHALRR
                     LIDTHGLPFGGKVHVDFVTGVPGGMPATTAALMAGVAMLPAEVTSWSATGIGRGHLPI
                     MAAALSAGGHLRVGMEDNVVFARGQVVQHNDELVVRAAELATLMQRPPLGTEAARELL
                     GVKDRRTR"
     gene            complement(6519..7544)
                     /locus_tag="PL014_05595"
     CDS             complement(6519..7544)
                     /locus_tag="PL014_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOT56599.1"
                     /translation="MTTSTVSSPLLDRPGAVAGEGADAGVAAHYGDPMREQRLLAEGL
                     AVVDLSHRGVVTVTGPDRLTWLHSMTTQHLTGLAPRTSRESLVLSPKGHIEHALHLVD
                     DGETTWITVEPGSAPALVAWLDSMRFMLRVEVADVTADWAVLGEPIGAESAPGEPLAW
                     VDPWPSLVGDTAAYGPVEGHPGEGRAWRELIVARPALEAAVGDRPLAGTWAAEALRVM
                     AWRPRLGAETDHRTIAHEVDWLRTAVHLHKGCYRGQETIARVHNLGRPPRRLVFLHLD
                     GSGHVLPDAGAPVRFGDREVGRITTVARHHEDGPVALAVIKRSIPEDVDLLVGETAAS
                     QTVVVSG"
     gene            complement(7541..8044)
                     /locus_tag="PL014_05600"
     CDS             complement(7541..8044)
                     /locus_tag="PL014_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOT56600.1"
                     /translation="MSDLGERLRARGKRLTSQRERVLGAVGRLGHATPDDIVDAVASD
                     GGGQVAASTVYRALDALQELGLVGHTHVDHRAPSYHLAEHATHLHVVCRGCGSVGEAP
                     LTMADELVARLDESLGFAAEVSHAAVHGLCRKCRAADPAEPHRAGDHPASDVPLSAHP
                     ESPKATP"
     gene            8163..9194
                     /locus_tag="PL014_05605"
     CDS             8163..9194
                     /locus_tag="PL014_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT56601.1"
                     /translation="MSEHGVGSLIEGLPDTQESRREQKRRDRRGSRRALIVMLSIFGL
                     IIGTGVAYVGYLAFTVGNNIDQGIELGERQPVTAPDGEPVAETGAGTNFLVLGSDTRP
                     GDAGRSDVIVLVHVPEDNSAVQMIHFPRDLYVDIPGHGKNKINAAYAFGREPLLVQTM
                     EQLLGVRIHHVARTDFEGFKNMTDAVGGVRVYAEEGGADGIVKGWNDLDGEQALAFVR
                     ERYTLSEGDISRGRRQLAFIKALMLEATKPENIANPITVARFANAATENLVVDKGLTT
                     RAMTDYAIALRNVRGGDVVFATAPFSGYGTSPQGASIDIVDEAGMAELGEALRTDSMD
                     TYLDVFVTP"
     gene            complement(9211..9720)
                     /locus_tag="PL014_05610"
     CDS             complement(9211..9720)
                     /locus_tag="PL014_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HOT56602.1"
                     /translation="MVFHLDTTLHPDVAPLAWLVGRWAGAGLVGYPTIESARFGQEIV
                     CSHDGRPFLEWRSQTWLLDDDGNQVRPLATEVGFWRMVPVHDDGTNAELLLTHHTGFV
                     EMYAGVASPAKVELRTDGVMRSPFAKEYTAGHRMYGNVNSKLMWVMDMAAMGQPLQSH
                     ISAELKRVE"
     gene            9733..10104
                     /locus_tag="PL014_05615"
     CDS             9733..10104
                     /locus_tag="PL014_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOT56603.1"
                     /translation="MDTPAASLVVKVTCGTEALERLNQGFTVAATALAAGVEVSLWLT
                     GDATFMAVPGRAELVDLPDAVPLAELRDLLLDGATVTVCGQCAARRGLTVDDLSSGAG
                     IRGAAAFVEEVMAPGARSLVY"
     gene            complement(10126..10992)
                     /locus_tag="PL014_05620"
     CDS             complement(10126..10992)
                     /locus_tag="PL014_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56604.1"
                     /translation="MPRVDEDSSDVTAAPEPETRAARRARRDAVLHEPVLPALATVPR
                     PVTVAATVVLAGLLVLALVADPVLLAAGLAWAGIVVAWGWPALHGSSSRFGSSLAIGV
                     PAVLAPAAGVASSEQPYLRLVPVALIIGLAIMFGHQILRRDGRPRLTDSIGVTSFGLA
                     VIALGTTWLPLSRGSFTTEVAVVAFVAIATASFADLGAGMSALRPWMLPAAMLLGGLG
                     AIIAASVIGGPGIAPAALVGFACAAVSHAMRRVHSVLPAITTVKSQMSSAAAALLVPG
                     VVAYGLSLLIIG"
     gene            complement(11000..11272)
                     /locus_tag="PL014_05625"
     CDS             complement(11000..11272)
                     /locus_tag="PL014_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOT56605.1"
                     /translation="MHTSGPGTDPAEMVTVRYWAGARAAAGVAEERLAGVATVDDLVT
                     HLAESRPGLLAVLPVCSVLVGGRASSGGDQIAPGAVVEVLPPFAGG"
     gene            11294..11992
                     /locus_tag="PL014_05630"
     CDS             11294..11992
                     /locus_tag="PL014_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT56606.1"
                     /translation="MAHLLLLTNTLAPSAEVVPALGLLSHHVRILPAEASVLVDAPQA
                     DVVIVDARRELAMAKSLCRVICTTGISIPLLVVVTEGGLAGLTSDWGIDDVILDTAGP
                     AEIEARLRLVMGRTSEAAEDETGPITAGALVIDEVTYSVRLRGRLLDLTYKEFELLKY
                     LAQHPGRVFTRSQLLQEVWGYDYFGGTRTVDVHVRRLRAKLGTDYEVLIGTVRNVGYR
                     FVPEVADSFSEIES"
     gene            11994..12974
                     /gene="mshD"
                     /locus_tag="PL014_05635"
     CDS             11994..12974
                     /gene="mshD"
                     /locus_tag="PL014_05635"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HOT56607.1"
                     /translation="MTRPPRTTRLSAIDDATASEIRALLALAEDADGVEAVSESFRLA
                     IGPAREGVVHLVRLDEDDAVVGYAQVAQAGGPDAVAELVVDPRHRRRGHGRALLDEVV
                     ALGARSVWAHGMLPAATALAAAAGLVVTRELYVMSRPLTADDAVDPVLPQGFSVETFA
                     DRGDVEELRALNAAAFAHHPEQGRLTRSDLEERIASEWFDANGLFYLLDENAPMDDPP
                     VAFHWTKVEPTTPPAVPRGEVYVVGVDPAYQGRGLGGPVTSLGLAHLARLGLAEVELY
                     VDGDNTAARRTYTRLGFRDTAVHAQYGTQPGTPSADAPSADLTEPDRIAP"
     gene            12971..>13652
                     /locus_tag="PL014_05640"
     CDS             12971..>13652
                     /locus_tag="PL014_05640"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851808.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOT56608.1"
                     /translation="MTAETEVTHEDVSLATPITARDAAADASADASIVSEVSIVSAAA
                     EETRNHQPRGSNGRFVGRPAGADGIPDAEDEALPADRFLDREISWLQFNERVLQLAGD
                     DNIPLLERCRYLAIFGSNLDEFFMVRVAGLKRRIATGIAVRSASGLEPREVLEQISLV
                     ASELMSMQARVYRDQVRPALATEGITIVRWDELSQEEHERIGEMFTSRVFPVLTPLAV
                     DPAHPFPYI"
BASE COUNT         1991 a         4835 c         4851 g         1875 t
ORIGIN      
        1 cgcatcatgc cggccgcgaa ggcgcacagc tgaccgggca ggtcctcctt ggacagaccc
       61 tcgtacggcc cggccgcgag gtggcgcacg acgacccccg gctccacctc gaccacgggg
      121 gactgggccg cacacgtcgt ccgggtgaac aggtccacgg cgatgtcacg ccgggccagc
      181 tgccgggcca cctcgagcac gtagacgttc atgccgccgg catccccctc tccgggctgg
      241 gcgagcggag aggtgtgcac ggtcaccatc gcgatgcggt gcaggcggcg ctgccgtggg
      301 tgcgtgctca tgaccaccga gtgtgtcacg gcatccgcgg ggtgtccgtg acggtcacca
      361 ccgcccgggg ggcccagacg tggatcgcga tgcgcccgtc ctggcgaaga tcgacggcac
      421 gaggcacctg gaccggaccg atcgacacgt cggtgccgag cacccaggtg aacgcgggtt
      481 gggcgatgac ggtcgggtca acccagtacg cctgctcgcg caggttgcgt tccggcaggg
      541 caggggcgat gccggtcgtc gccggccgcc agtcgaggtg gatcgccagg gcatcgaccg
      601 ccggcacgag gacgtccatg cggatcgacc cgtcggtcgg ctcgaacttg atccgcagcg
      661 gcctgctcgt cgaggagtcg cgcaccacgc ccaggtactc gaccacgtcg ccgacccgac
      721 gcaccgcgtc gatgcgcacg gtggacagga ccccactcac cgtctcgcgc ggatgatccc
      781 caccctcggt caccgtcccg aacatcgccg acaccggggc accgcgcgtc accgtgtccg
      841 cgagcacctc gctcccgcgg gcgagccgga accccgacgt gaagagggac accgacaacc
      901 cggccccgag cgactgcacc ccgcgcgccc cctgcgcgac ctcgagaagc cccggttcga
      961 cgtcgacgat cccgatgtcg cgagccatga cacgcggcac ccacgaggtg agtccgagca
     1021 ccagcgcgcc gctcagcgag acgaccaggg ccagcatcga caggctcact cgcccgtcac
     1081 ggctccgtcg aacccaccag tccagcaccg tctcagccta cgtccggcag gttggcggct
     1141 cgagccaccg aactgccgga cgtacgctga cccggtgaca cggcagcggc cacacggggt
     1201 gatcacccgg ggcacgacca accccaaccg gttgcggcgc tgtgaccgct ggctcgccgg
     1261 tccgcagggg tggcggctgc gcgcccaccc gggcccgccg gtcgtcgtcg acctcggcta
     1321 cggagcctcc cccgtcaccg cggtggaact ccacgaccgg ctgagcgcgg ttcgcccgga
     1381 cgtcgaggtc gtcggcatcg agatcgatcc ctcgagggtg gccgccgccc gccccctcga
     1441 gcgccccggt ctgcggttcg ccgtcggcgg cttcgaggtt cccctgccgc agggtcgccg
     1501 gccggtcatc gtgcgggcgt tcaacgtgtt gcggcagtac gacgaaggtg aggtggccgc
     1561 cgcgtgggac gccacggcat ccagactcgc ccccgacggc ctgctcgtcg acggcacgtg
     1621 cgacgagctc gggcgggtcg cggcgtgggt ggcgctcacg gcatccggac cggtgagcct
     1681 cacgatcagc ctgcgcctgc gtgacctgga gcgacccggg atcgtcgccg aacggctccc
     1741 caaggcgctc atccaccgca acgtgccggg cgaacggatc caccggctgc tgaccgagct
     1801 cgacgaggcg tgggcgcggc aggcaccaca tgcggcctac ggggtgcgcc agcggttcat
     1861 cgcgacggcc ggggcactcc gggatgccgg gtggcccgtt ctcgacggac ccgcacgctg
     1921 gcgggtcggc gagctcacgc tcgcgtggga agccgtggca ccgcgcgcgc tctgaggccc
     1981 tcgccccgcg cggcgaccgg gtcaccaggg gccgtagggc ccctggttgc cgctgccgcg
     2041 acccatgacc tggtcgagtg ccggcttgac gtcggcgagg tagaccccgg cggcgacgat
     2101 ggcgatgatc gcgaggaagc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nccctcgtcg agcaccgatc gctcgccgtg caggatgcgc aggtcggcga tcttgcgggc
     2281 cagggtgtcg acctgggccg gcccgtcgtc gtgggtgacc ccgacgaggg cgacgaggcc
     2341 ggggcgggtc agctcgccga cgacctcgcc ggcgacgacg acccgggcgc tggacacccg
     2401 ctggagaacg gctctcatcg cggcgtcacc ccgcgtgggt ggtcgagccg ccgacgctca
     2461 tggtgccgtc gaggtccgcc gccacgacag cgcccaccgg ttgcccgtga ccgaggatcg
     2521 gggccgactc gagccgctcg tacgcgctgg actccacgcc caggcggcgc aggaccgcgg
     2581 ccagcacgac cgggcgggcc cgctggccac cgtcggcgat cttcagggcg attccgcggc
     2641 cgtcggccaa cccgacggcg tacacggctt cggccccgtc cttggcgatg acgccctgcg
     2701 tgccctggat cagcgccgtg acgtcgcgcc gggtgcctcc caggtactcg gggtgctggc
     2761 ggatcgcgtc ggcgacgcga ccctcgtgcg tgcccgggtc ggccgcggcc acgcgcccga
     2821 acgcccgcgc cagcccggcg agcgagatcg ccatcacggg tgcaccgcag ccgtcgacgg
     2881 ccatcgccgc gaccggctcg cccgcgaggt cggcgatggt ctgcgccatg agctgctgaa
     2941 gcggatgccg tgggtcgcga taggtcgcga tgtcccagcc gttgacccgg caggtcgcga
     3001 gcatggccgc gtgcttgccc gagcagttct gcgccagcga gcgggccgcg cgcccggcgg
     3061 cgatccacac gtcgcgctcg tccgggtcgt agggcaggtc ggcagtgttc tgcaggtcgg
     3121 cctcggtgag tccggctgcg gcgagcatgt cgcgcacggc gtcgaggtgg aagggctcgg
     3181 ccgagtggct cgcgctggcc agggccaggt ggtgcggcgg caggtcgagc ccggcccgca
     3241 ccatcgccac ggactgcagt ggcttgttcg aggagcgcgg gaacaccggg tcgtgcgggt
     3301 cgccccacgc ctggagcacc gagccgtcgg atgccgtcac ggcgacgctg gcgcggtgca
     3361 ccgactccac caccccgccg cgggtcacgt ggaccagcac cggcgcctcg gcgagcgcgg
     3421 gtggtgtcac cgtggagacc gggggacctg acggatgggc tgaactcgag gtctgctggg
     3481 gcacgaacgt gaccctatcc gcgggaccgg gtggacggca tccaccgacc gcaccggtcg
     3541 tgcctgcgac cgcagacacg aacgaccggg gccggcaggg ccggccccgg tcgtcggggt
     3601 gtcaccggga aggggtggac cggtgacggt caggtcgggc gtggccgacc acgtggtgtc
     3661 gctcaggcgg tgacggtgac ggtcgcggac gcggccggcg tgtcagcggt cttcgcggcg
     3721 ggcttcgcag ccggcgcgga ggtgcgcttg gcggcgggct tcgtgctcac cttgacggtg
     3781 cgcttggccg gggtgcgctt ggccgcgggg acgtcgccga ccttctcggc agccttctcg
     3841 gtcgccttct ccgcagcgac agcggtcttg cgagcgctgg tcgtggcggc cttggccgag
     3901 ctgacggcct tcttggaggc gttcttggtc gtggtcgacg tgcgcttggc cgcggtgcgg
     3961 gtgcgcttgg cggactcggt gacctcggcc tcgacggtct cgagaccctc ggtgacggag
     4021 ccggtgacga cctcggccag cttcacggcc tccttgcggg tggtcgtgat ggtggccttg
     4081 gcggagcgct cgatgtcggc cgccgcgttg cgggcggagg tgacggcacc cttggcctgc
     4141 gcgaccgagg tctcggcctg cgcgaagagg tccttggtgg acttctggtt gcggatgcgg
     4201 ttcacgaggt tcttgccgcg cacggcgagc tcgtcgtagc cctcggcgac cttgccgccg
     4261 acgaccatcg tctcgttgag gacgaccgca gggatctcct tgacgccgtc gaccagggtg
     4321 gtcgcacggg cctggacctt ggccggggcc aggtcggcgg agagctcgac gcggaccttc
     4381 tcggcgcgga tgcgggcctc gcggaccttc tcgacggcca ggtcagtggc gccgacagcg
     4441 gcgaagaagg gggtggcgtc gacggtgttc ttgatgtcgg tcatgactga catgggattc
     4501 tccttggtgg tggtgcgtga gttcaggggg gtcggcatca ttgccgtcag ttgtcctcga
     4561 cgtaggactc gtagatgtcc aggagggtct tgcgctgccg ctcggtcagg gccgggtcgg
     4621 cggcgatggc gcctcggacg tcgggggtct cggagtgcgg ggtgacctcg gcgtcgagga
     4681 ttccggcctt gacgtagagg ctctcggccg agacctcgag acccttcgcg agctgctgga
     4741 ggatctcggc actcggccgc ttgagccccc gctcgatctg ggacaggtag gggttggaca
     4801 ccccggcgag ctcggacagc tgacgcaggg acagcttggc ggcggtgcgc tgctctcgca
     4861 ggtagtcgcc gaggctgctg cccaggtcgt tcagcggatt cttgctcacg tgacaagtat
     4921 gcttgctcta gttagcaaaa tgcaaactgg agcaagcgtg agcccgctca caccgttgag
     4981 cgctcaactc tcagcgctca cgcggctcgc gggatcagac cccggcaggc tggcgctggc
     5041 gcagcgagat gtcgttgcag gccgcgcagg tctcctcggg caccaggccc gcgcgcatga
     5101 gcaggccgaa cgcgtagcag ccggcgcaga agccgaaggc cgactccagg gtggcgaaca
     5161 cgagcaggat gccgagcagc gcagtcgtca cgagaggcag gccagccacg aggaagcccg
     5221 tggcgacgag ggtgagcacc aggccgatgg cctgggcgaa ccgcttcggc gggccgggga
     5281 cgagcacggg ctcaccgagg cggggggcga tcaccttggt ggcgagctgc ccgaaggggt
     5341 cgaaccgcgg gccgctggcc actcgcaggg cgaacccgac ggcgagcacg gcactcagcc
     5401 agagccagcc ggtgagcagc gccaccgcag cgacgagcac cacgagtccc gccacggccc
     5461 gggcagcctt ctcgttgacg acggccggga attcaaaagg agtgcgcacg tcagccataa
     5521 ccagtgatta tgcgccagac attcccgatg gctagacacc cgtccgaggc tcggacgggg
     5581 cggaacgtct ccgtcgtcac tggtgcgacg gagaaccgtc gtcagcgagt gcggcggtcc
     5641 ttgaccccga gcagctcacg ggccgcctcg gtgccgagcg gcgggcgctg catgagcgtg
     5701 gccagctcag cagcccgaac caccagctcg tcgttgtgct ggacgacctg tccgcgggcg
     5761 aacaccacgt tgtcctccat acccacgcgc aggtgaccac cggccgacag ggccgcagcc
     5821 atgatcggca ggtgcccccg cccgatgccc gtcgccgacc acgaggtgac ctcggcgggc
     5881 aacatggcga caccggccat cagggcagcc gtcgtcgccg gcatcccacc cggcacgccg
     5941 gtcacgaagt cgacgtgcac cttcccgccg aacggcaggc cgtgggtgtc gatgaggcgc
     6001 cgcagcgcgt gcacgtggcc gaggtcgaac agctcgaact cgggcaccac ctcgcgctcc
     6061 tgggcctgca cgtacaggtc gctcatgaac ccccacgggt tcaggaagac gtcgtcaccg
     6121 aagttggtgg tcccacacgt gagcgagcac gagtccggct cggcgtcgag caccgtcagc
     6181 cgcgtctcga gcgggtcgtg caccgaaccc cccgtcgaga gctggacgat gaggtcggtc
     6241 tccgagcgca cggcatccac cgacgccttc agcaacccac ggtccagggt cggggcgtgg
     6301 tcggcgtcgc ggatgtgcag gtggatcagc gccgccccgg ccgcctcgca gcgccgagcg
     6361 gtctcgacga gctcgtcgag ggtcgtcggc agctggggca ggtccgcctt ggcgtgctcg
     6421 gcgcccgtgg gggcgacggt gatgagggtc ccgaccgggg tcgatgaggt ggccatggac
     6481 cccatcatgt caggtcgcta ccgaagattc ttcggggatc agccactcac gacgacggtc
     6541 tgcgatgccg ccgtctcccc gacgagaaga tccacgtcct ccgggataga gcgcttgatg
     6601 accgcgagcg cgaccgggcc gtcctcgtgg tgccgagcga ccgtggtgat ccgccccacc
     6661 tcccggtcgc cgaagcgcac cggcgccccg gcatccggga gcacgtgtcc cgacccgtcg
     6721 aggtgcagga agaccaggcg ccgcggcggc cgcccgaggt tgtgcacgcg ggcgatggtc
     6781 tcctggccgc ggtagcagcc cttgtgcagg tgcaccgcag tgcgcagcca gtcgacctcg
     6841 tgcgcgatgg tgcggtgatc ggtctcggcg ccgagccgcg ggcgccaggc catgacgcgc
     6901 agcgcctcgg ccgcccaggt accagccagg ggtcggtcgc cgaccgcggc ctcgagagca
     6961 ggccgcgcca cgatgagctc acgccacgca cgcccctcgc cggggtgccc ttcgaccggt
     7021 ccgtaggccg ccgtgtcacc cacaagggag ggccaagggt cgacccacgc gagcggctcc
     7081 cccggcgccg actcggcgcc gatgggctca ccgagcacgg cccagtccgc cgtgacgtcg
     7141 gcgacctcga cccgcagcat gaagcgcatc gagtccagcc aggccacgag cgcaggcgcg
     7201 ctcccgggct cgaccgtgat ccacgtcgtc tccccgtcgt cgacgaggtg cagggcgtgc
     7261 tcgatgtgcc ccttgggcga gagcacgagc gactcgcgcg acgtgcgagg tgccagcccg
     7321 gtgaggtgct gcgtggtcat cgagtgcaac cacgtgagcc ggtcgggccc ggtgacggtg
     7381 acgacccccc ggtgcgacag gtcgaccacg gcgagcccct cggccagcag tcgctgctcg
     7441 cgcatcgggt cgccgtagtg ggcagccacc ccggcgtcgg cgccctcacc cgcgaccgcg
     7501 cccgggcggt cgagcagggg ggaactcacg gtggacgtgg tcatggtgtg gccttcgggg
     7561 actcggggtg tgcggacaac ggcacgtcgg atgccgggtg gtcaccggcg cggtgcggtt
     7621 cggctgggtc ggccgcgcgg cacttccggc acagtccgtg cacggcggcg tgcgacacct
     7681 cggcggcgaa tcccaacgac tcgtcgaggc gggccaccag ttcgtccgcc atggtgagcg
     7741 gggcctcgcc caccgagcca cacccccggc agacgacgtg caggtgcgtg gcgtgctcgg
     7801 cgaggtggta gctcggcgca cggtggtcga cgtgggtgtg accgaccagg cccagctcct
     7861 gcagggcgtc cagtgcgcgg tacaccgtcg atgcggcgac ctgtccaccc ccgtcgctcg
     7921 ccacggcatc cacgatgtcg tccggggtcg catgaccgag ccgccccacg gcgccgagca
     7981 ctcgctcacg ctgcgaggtc agccgcttgc cgcgggcccg cagtcgctcg ccgagatcgc
     8041 tcaccccacc aatgtatgcc gccacgacgg cgggccacgc atccctgcac ccggcggaga
     8101 tgcaccggtg caggctgcgg agcgtccagc gggcgtccag agaaggctcg tagactggtg
     8161 gagtgagtga acacggcgtg gggtccctga tcgaagggct tcctgacacc caggagtccc
     8221 gccgcgagca gaagcggcga gatcgtcggg gctcccgacg ggcgctcatc gtcatgctca
     8281 gcatcttcgg cctcatcatc ggaaccggcg tggcctacgt cggctacctc gcgttcacgg
     8341 tcggcaacaa catcgaccag ggcatcgagc tcggcgagcg ccagccggtc accgcacccg
     8401 acggcgagcc ggtcgccgag accggcgccg gcaccaactt cctcgtcctc ggctccgaca
     8461 cccgccccgg cgacgccggg cggtccgacg tcatcgtgct cgtgcacgtg cccgaggaca
     8521 acagcgcggt gcagatgatc cacttccccc gcgacctcta cgtggacatc cccgggcacg
     8581 gcaagaacaa gatcaacgcc gcctacgcct tcggccgcga gcccctgctc gtccagacca
     8641 tggagcagct gctcggcgtg cgcatccacc acgtcgcccg caccgacttc gagggcttca
     8701 agaacatgac ggatgccgtg ggcggcgttc gcgtgtacgc ggaggagggc ggcgccgacg
     8761 gcatcgtcaa gggttggaac gacctcgacg gcgagcaggc cctggctttc gtccgcgagc
     8821 gctacaccct cagcgaaggt gacatctcgc gtggtcgacg ccagctggcc ttcatcaagg
     8881 cgctgatgct cgaggcgacc aagcccgaga acatcgcgaa cccgatcacc gtcgccaggt
     8941 tcgcgaacgc tgcgaccgag aacctggtcg tcgacaaggg cctgaccact cgggcgatga
     9001 ccgactacgc gatcgcgctg cgcaacgtgc gtggtgggga cgtcgtcttc gcgaccgccc
     9061 cgttctccgg gtacggcacc tcgccccagg gtgcatcgat cgacatcgtc gacgaagccg
     9121 ggatggccga gctcggcgag gcactgcgca ccgactccat ggacacctac ctcgacgtct
     9181 tcgtcacccc ctgacgccca gcccgcgggg tcactcgacg cgcttgagct ccgcggagat
     9241 gtgcgactgc agcggctgcc ccatggcggc catgtccatg acccacatga gcttgctgtt
     9301 gacgttgccg tacatgcggt gacccgccgt gtactccttg gcgaaggggc tgcgcatgac
     9361 gccgtcggtg cgcagctcga ccttggcggg tgaggccacc ccggcgtaca tctcgacgaa
     9421 cccggtgtgg tgggtgagca gcagctcggc gttcgtgccg tcgtcgtgca ccggcaccat
     9481 tcgccagaag ccgacctccg tcgcgagcgg gcgcacctgg ttgccgtcgt cgtcgagcag
     9541 ccaggtctgg gaccgccact cgaggaacgg gcggccgtcg tggctgcaga cgatctcctg
     9601 tccgaaacgg gccgactcga tcgtcgggta gccgacgaga ccggcgccag cccagcggcc
     9661 gaccagccag gcgagcgggg cgacgtccgg gtgcaaggtg gtgtcgaggt ggaagaccat
     9721 acgctgcatc ctatggacac cccggccgct tccctcgtcg tcaaggtcac ctgcggcacc
     9781 gaggccctcg aacggctcaa ccagggtttc accgtggcgg cgaccgctct ggccgccggt
     9841 gtcgaggtca gcctctggct caccggcgac gcgacgttca tggcggtgcc gggccgagcc
     9901 gagctggtcg acctcccgga tgccgtgccg ctggctgagc tgcgcgacct gctgctcgac
     9961 ggggcgacgg tcacggtgtg cggtcagtgc gcggcccgcc gtgggctcac ggtcgatgac
    10021 ctctcgagcg gggccggcat ccgtggggcg gccgccttcg tcgaggaggt catggcgccc
    10081 ggcgcccgat ccctcgtcta ctgaagcgcc ggggcgcgca ggccgtcagc cgatgatcag
    10141 cagggacaac ccgtaggcca cgacgccggg cacgagcagc gcagcggcgg cgctggacat
    10201 ctggctcttg accgtcgtga tggccggcag cacgctgtgg acccggcgca tcgcgtgcga
    10261 gaccgcggcg caggcgaacc ccacgagcgc tgccggagca atgcccgggc ccccgatgac
    10321 ggacgcggcg atgatggctc ccagcccccc gaggagcatg gcagccggca gcatccacgg
    10381 ccgaagcgct gacatcccgg cgccgaggtc ggcgaaggac gcggtggcga tggccacgaa
    10441 cgccaccacc gcgacctcgg tggtgaacga accgcgcgac aacggcaacc acgtcgtccc
    10501 cagcgcgatg acggccagac cgaacgaggt gaccccgatc gagtcggtga gccgcggtcg
    10561 cccgtcacga cgcaggatct ggtgcccgaa catgatggcg agcccgatga tgagggcgac
    10621 cgggacgagc cgcaggtagg gctgctcaga gctggcgacc ccagctgcgg gtgcgagaac
    10681 ggccgggaca ccgatcgcca gcgacgaccc gaaccggctc gacgagccgt gcagcgccgg
    10741 ccagccccag gcgacgacga tcccggccca ggccaacccg gcagccagca gcacggggtc
    10801 ggcgacgagg gcgagaacga gcagacccgc caggacgacg gtcgcggcga cggtgacggg
    10861 ccgcgggacg gtggccaggg cgggtagcac cggctcgtgg agcaccgcgt cccggcgggc
    10921 gcgacgggcc gcacgggtct ccggctcggg ggccgcagtc acgtcgctcg aatcctcgtc
    10981 cacgcgcggc atcctcccat catcccccgg caaagggcgg gagaacctcg acgaccgcgc
    11041 cgggcgcgat ctggtcacca cccgagctcg cccgaccgcc gacgagcacc gagcacaccg
    11101 gcaggaccgc gagcaggccg gggcgggatt cggccaggtg agtgacgagg tcgtcgacgg
    11161 tggcaacccc cgccagccgc tcctccgcga ctcccgcggc agcccgggcc cccgcccagt
    11221 agcggaccgt gaccatctca gccggatcgg tgccgggacc tgacgtgtgc atgaccacct
    11281 atcctgatgg gcaatggccc acctgctgct gctgacgaac accttggcgc ccagcgccga
    11341 ggtcgttcct gcgctcgggc tcctgagcca ccatgttcgc atcctccctg cggaagcctc
    11401 cgtcctggtc gacgcgccac aggctgacgt cgtcatcgtc gacgcccgtc gcgagctcgc
    11461 gatggccaag tccctgtgcc gggtgatctg cacgacgggc atctcgatcc cgctgctcgt
    11521 cgtcgtcacc gaggggggcc tggccgggct gacgagcgac tggggcatcg acgacgtcat
    11581 cctcgacacc gccggaccgg cagagatcga ggcccgcctg cgcctggtca tggggcgcac
    11641 gagcgaggcc gccgaggacg agaccgggcc gatcaccgcc ggcgccctcg tcatcgacga
    11701 ggtgacctac tcggtgcggc tgcgggggcg gctgctcgac ctcacgtaca aggagttcga
    11761 gctgctgaag tacctcgcgc agcacccggg gcgggtcttc acgcgatcgc agctgctcca
    11821 ggaggtgtgg ggctacgact acttcggggg cacccgcacg gtcgacgtcc acgtgcggcg
    11881 gctgcgcgcc aagctcggca ccgactacga ggtgctcatc gggaccgtcc gcaacgtcgg
    11941 gtaccgcttc gtccccgagg tcgccgactc cttctcggag atcgagtcct gacgtgacgc
    12001 gccccccgcg cacgacgcgc ctgtccgcga tcgacgacgc cacggcatcc gagatccgtg
    12061 ccctgctggc gctggccgag gacgccgacg gcgtcgaggc cgtctccgag tcgttccggt
    12121 tggcgatcgg tccggcccgc gagggcgtgg ttcacctggt gcgcctggac gaggacgacg
    12181 ccgtggtcgg gtatgcgcag gtggcgcagg cgggcgggcc ggatgccgtg gccgagctgg
    12241 tcgtggaccc gcggcatcgt cgtcgcgggc acggtcgcgc cctgctcgac gaggtggtgg
    12301 cgctgggtgc acgcagcgtc tgggcccacg gcatgctgcc cgcggcgacg gccctggccg
    12361 ctgctgccgg gctggtcgtc acgcgtgagc tgtacgtcat gagccgaccg ttgacggccg
    12421 acgacgcggt tgatccggtt ctgccacagg gtttctccgt ggagaccttc gctgaccgag
    12481 gagatgtcga ggagctcagg gccctcaatg ccgccgcctt cgcgcaccac cccgaacagg
    12541 gccgcctgac caggtccgac ctcgaggaac gcatcgcgtc cgaatggttc gacgccaacg
    12601 ggctcttcta cctcctcgac gagaacgcgc cgatggacga cccaccggtt gccttccact
    12661 ggaccaaggt cgagcccacc accccgcccg ctgtcccgcg gggggaggtt tatgtcgtcg
    12721 gggtcgaccc tgcgtaccag gggcgcgggc tcggcgggcc ggtgacctcg ctcgggttgg
    12781 cccacctcgc gcgcctgggc ctggccgagg tcgagctcta cgtcgacggc gacaacaccg
    12841 cggcccgtcg gacgtacacg cggctgggat tccgggacac cgcggtccac gcccagtacg
    12901 gcacccagcc cggcaccccc tctgctgacg ctcccagcgc cgacctcacc gaacctgaca
    12961 ggattgcccc atgacggcag agaccgaagt cacacacgag gacgtttcgc tggcgacgcc
    13021 gatcaccgcg cgcgatgccg ccgccgacgc ctcggccgac gcgagcatcg tcagtgaggt
    13081 ctcgatcgtg agcgccgccg ccgaggagac gcgaaaccac cagccccgtg ggtccaacgg
    13141 gaggttcgtc ggccgccccg ccggcgcgga cggcatcccg gatgccgagg acgaggccct
    13201 gccggccgac cggttcctcg accgcgagat ctcgtggctc cagttcaacg aacgggtcct
    13261 gcagctcgcc ggtgacgaca acatcccgct gctcgagcgc tgccgctacc tggcgatctt
    13321 cgggtccaac ctcgacgagt tcttcatggt gcgcgtcgcc ggcctcaagc gccggatcgc
    13381 gaccggcatc gccgtgcggt cggcatccgg tctggaacct cgcgaggtgc tcgagcagat
    13441 ctcgctcgtc gcgtccgagc tgatgtcgat gcaggcccgc gtctaccgcg accaggtgcg
    13501 cccggccctc gcgaccgagg gcatcacgat cgtgcgctgg gacgagctct cgcaggagga
    13561 gcacgagcgc atcggcgaga tgttcaccag ccgcgtgttc ccggtgctca ccccgctcgc
    13621 cgtcgacccc gcgcacccgt tcccgtacat ct
//
LOCUS       DAOSOC010000049        13248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4711_length_13248_cov_11.145229, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000049 DAOSOC010000000
VERSION     DAOSOC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4711_length_13248_cov_11.145229"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1422
                     /locus_tag="PL014_05645"
     CDS             16..1422
                     /locus_tag="PL014_05645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOT56609.1"
                     /translation="MSDQVTPLDAIFLMGETPETLMHVASLLHFTYPEGVRRTTFLRQ
                     LVNDLRAAPIESPWFYRLQTRTLMRQPVHRWVEDSEFDIDYHVRHSALPSPGGERELG
                     VLVSRLHSNQLDFRRPPWEMHVIEGLSGNRFAIYTKIHHSLVDGFTGMRILQRGLATT
                     PDDLEHPFFFSTRKPSRPEGARRQADPVGDVTSILRSLASSVGTTPSVLKALVETQVG
                     RGRTPTKAVTSYQAPDSILNSRTGRSRRFATQEYDLGRLRAIAKARKATLNDVLMAIC
                     AGGLRRWLDDLEALPERPLIAFIPVNVRPPESEGGGNAVGATLVSLATDIEDPLERLS
                     AITASSRAAKARMRGMSADAVMAYSALLLAPAGLQVAKAMSGLPGPVPHTLNVCISNV
                     PGPKEHLYLRGARLEATYPVSIPGHSMALNITAHSYAGTLDLGFIGDREALPHLQRLA
                     VRAGEALEELEAAVAGLD"
     gene            complement(1457..1876)
                     /locus_tag="PL014_05650"
     CDS             complement(1457..1876)
                     /locus_tag="PL014_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT56610.1"
                     /translation="MAIALNPYLNFPGTTREAMTFYQSVLGGELQISTFGDFGAQGPE
                     ANGVMHSHLETEAGMTFMASDMPPGQEDSYVAGNTITMSISGDDSEAMRRYWDGLAEG
                     GTVQMPLERQMWGDDYGMLVDKFGVAWMVNIAGEQQG"
     gene            complement(1936..2397)
                     /locus_tag="PL014_05655"
     CDS             complement(1936..2397)
                     /locus_tag="PL014_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56611.1"
                     /translation="MTRRHLMLIALIWLPFLMLPAFAAFGDTSYPHIAFHVLAIPMLV
                     GAAVLVRRLLAQTESTAQRRICKALLLFVPLAVLGHLAELATALARFADDGFVNRDTA
                     DVWVSGPHFHAANVTIPSMMLAMVTVLVLAVTVAVQGRSRKESSPSVGTRL"
     gene            complement(2472..3884)
                     /locus_tag="PL014_05660"
     CDS             complement(2472..3884)
                     /locus_tag="PL014_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT56612.1"
                     /translation="MTRRTSPTPVEVTAATVMIAVAGLTVTAGVVAEPGPPGASPWVL
                     VWALVTAVVLLLAHRGLDPGWVAWRRATLGLVGVALAYPAVAATLALAVASDSTGGSW
                     IRLLAALATVGHVPVIAALSLLPLLAVRYIGNGLPPRLITVVLALATVDAVALALLAP
                     PMWPAGLTPLVESDTAVAVGLVVNLVFLASTLVGPVVATRAARDAVGEARPRLVLVAL
                     AALSGPVLVLGCGVLGALAAGPMGAEAPAVMVLCGMYAALATVSAGTSRALRSPLALR
                     TRTISWTAVVLVALIAALVAVTAVVLVDADGTLGSVLAALLAVAVMVGTRPLESRVAR
                     ALVSPALDRSSLDRSSLDPPPWDPPALVPLALDGALDEPEPEPSDAIDGRPPVPGPSE
                     SQPLPVLSPREREVLALLAEGLSNAGIAARLVLSERTVDAHLRSVFSKLALPESPHDN
                     RRVHAVLTWHDAHREGIRAS"
     gene            complement(4019..5539)
                     /locus_tag="PL014_05665"
     CDS             complement(4019..5539)
                     /locus_tag="PL014_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HOT56613.1"
                     /translation="MSAPPVTAGAPSGVARRMVRVVYPHQLFAAHLDAPPGTRFVLVE
                     DDLLFRHQPFHAQKLVLHRASSRHFVERLRDAGFDVDVLETDAVRPSGGRLAAHLAGL
                     RPDALSVVDPVDDWMLRDLRRSVADAGVGFGPHDVLESPNFLTTRPDLVDWFGDRRAR
                     MQTFYQWQRRRLDVLVEDGQPVGGRWSFDTENRKKLPRGHVVPSWSGAERTAEVDEAI
                     SWVQGEFPAAPGRAASFAWPVTHEQARAALQEFVDERLAQFGPYEDAVSTQHPVLFHA
                     AITPALNCGLLDPREVLDAVLTGAAEQGVELASLEGFVRQLIGWREYMRGTYHLFGRR
                     MRVANHLGHTRSLAPGWWGGTTGLDPVDHAIEGVLERGWAHHIERLMVLGNAMCLLRT
                     DPVEVYEWFMAMFVDAYDWVMVPNVHAMSQFAAGAAVTTKPYVSGSAYLRRMTDHGPG
                     PWREDWDALYWTFVEDHREVFEGNPRSRMVTRTWDGMDAVKRDSLTDRGRAVIARL"
     gene            complement(5536..6798)
                     /locus_tag="PL014_05670"
     CDS             complement(5536..6798)
                     /locus_tag="PL014_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT56614.1"
                     /translation="MDADVVVVGAGLAGLRCAHVLAQAGRAVVMLETAHAVGGRQRTD
                     EVDGFLLDRGFQVLNPAYPAVQRWVDLEALVLRPFGAGLQVRRERGLVELTHPLRAPG
                     GLAATLRSGLLSPRAVAALARWAGPAVVAPRRVIAGALAADRSLREGLDRAGVRGPLR
                     TEVIEPFLAGVLAEDRGETSEAFARLLVRMFALGVPGVPANGIRALPEQLAGRARAAG
                     VDVRLGERVTSLSADAGRTVLTLAGGSRVSARAAVVAVGPDAVAGLLPLPAPATKGLQ
                     TWWFDAPQAPTASTLLMVDGRRRGPIVNAAVMSNAAPSYAPAGRHLVQVTCLLAPDGT
                     GGTEAEVRRQAGEMFGASPADWGLLRRDDILHALPTQPAPLRTVSRARVSPGVYVAGD
                     HRDTASIQGALVSGDRVARTVLADLQGT"
     gene            6965..>7176
                     /locus_tag="PL014_05675"
     CDS             6965..>7176
                     /locus_tag="PL014_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56615.1"
                     /translation="MSAETTALKLDAPETTASVESTLTRADSLLETTFLELASALDQA
                     AADNAPDEASPTAGPLGVDLVGELAER"
     assembly_gap    7177..7276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7277..8247
                     /locus_tag="PL014_05680"
     CDS             <7277..8247
                     /locus_tag="PL014_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT56616.1"
                     /translation="SPTAGPLGVDLVGELAERSRNHGKRLRPTLAHWGWVVGGAEPGS
                     HTDLVRIAAALELLHLFALIQDDVMDRSDSRRGRPTLHVVAAQRHRDEQGLGDDTLFG
                     DSVATLVSDLALSEATLLVAPTAATVRAAWRLMAVELVEGQLLDVTHTAGRRRDHATS
                     RRIARFKSGRYTITRPLQLGALVGGADEHTVRGLIAWGDLIGDAFAVRDDVLGVWGDP
                     TQTGKPAGDDLRSGKPTELLTWAAELLPPSDRPLLDACDAGTLDDAGVDALQQAMVAA
                     GVRDRAEQAVADLSRRAHARLDALAVEPRADAALRGLADAIAWRCS"
     gene            8244..9752
                     /gene="crtI"
                     /locus_tag="PL014_05685"
     CDS             8244..9752
                     /gene="crtI"
                     /locus_tag="PL014_05685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOT56617.1"
                     /translation="MKVLVVGAGLSGLAAACHLTAAGHDVTVLEREDVVGGRAGLLRL
                     GDFRLDPGPVVMTMPELLHDPIRAVGGDPDSLITMRHLDPAYRAVYPDGSELHIRADM
                     DDLREEIRTKVGPADAAGFDRFLAWLEQLYDTEFDTFVDHNYTSPLDLVRSPATALKL
                     LRLGGLGALGPSVDRFFTDDRLTRIFSFQALYAGISPAKARAVLAVITYMDTVRGVFY
                     PEGGMHAVPRAMAQALTDAGVPIHLDVEVSEILRRGDGAVAGVATTGGERLAADAVVA
                     TVDLPVVYDRLLPGIRAPRTIRTGSYSPSAVVWHVGASGTPGPHLRHHNIHFGTDWDG
                     AFEAIIDRGELMPDPSRLVTVPSVTDPTAAPAGSTAMYVLEPVPHTGAGLDWRREAGP
                     MRERLLRFLEDGGYPTDIREEAFITPDDWRAQGMHLGTPFALAHTFRQSGPFRPSNVD
                     RRVPGLVFAGSGTQPGVGIPMVLVSGKLAAQRVQQYAGGARPVARTPRTVAR"
     gene            9749..11290
                     /gene="idi"
                     /locus_tag="PL014_05690"
     CDS             9749..11290
                     /gene="idi"
                     /locus_tag="PL014_05690"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOT56618.1"
                     /translation="MTEPTPATSPEVLAAGYARCAEITREHGTTYYWGARLLPAASRR
                     HVHAVYTLARLADDIVDLAGPEPGPETAAELDAFEASFWQALADGTYTDPVMAAVATS
                     VRECGIADECFTRFFGAMRSDLTQRTYETWADLLAYMDGSAAVIGEMMLPVLNPTTDA
                     VVPARALGLAFQLTNFLRDVGEDLDRGRVYIPQEDLRRFGADPHARVVTPQWRELMAF
                     QIERNRRLYREADAGIPILPGASRRCVATARVLYARILEGIEAADYDVFTSRARVPTA
                     RKAALAARMVTSRDPMRLVARDRAAVDPFGGAWVSDDPVVLVDEGGAPIGQGRKSAIH
                     HDDTPLHLAFSCYVLDPAGRLLVTTRAASKPSFPGVVTNTVCGHPTPGEDLAQAVRRR
                     ARSELGVEVGDLRLVLPEFRYRAQMNGIVENEVCPVFVGWATSDSTAPDPEEVDDVQW
                     VPWEQFRDEVLEGVRTVSPWATEQVPLLAALGPDPRSWPAGSRAALPPAATLGPGADA
                     LAPAS"
     gene            11356..12111
                     /locus_tag="PL014_05695"
     CDS             11356..12111
                     /locus_tag="PL014_05695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT56619.1"
                     /translation="MSTSAPSTQGWVLADAFDRAAPTYDAMVALSPGYHDQLLGAAGA
                     LLDRLPAPDGSALKVLDLGCGSGASTQALLEQWRTRGGAADLLSVTGVDMSEGMVAQA
                     RAKSWATGVDLVVSDAVTYLEALPDGSVDGILACYLLRNVPDRERLVREMARVLRPGG
                     GVVIHDYSVQGSTRAKAIWATICHGVIIPLAVIKRSDVPLHRYLYTSVRDFDSVARIC
                     ERLLGAGMVDVRHRTYPGWQRDVIHTVGGSAPS"
     gene            12162..>13248
                     /locus_tag="PL014_05700"
     CDS             12162..>13248
                     /locus_tag="PL014_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT56620.1"
                     /translation="MFHPPPAPTTPVDAVPASARHVVVVGGGIAGLTSALALAERGVR
                     VTVLERDQRLGGRVRAWPVDGPGSDAWMSRGFHAFFRQYYNLRAVLRRTDPTLSRLRP
                     LDDYPLVRDGGGQDSFAKIPRTPPWNLAAFVARSPSFDLRGLAGVDVEAALGLLDVDF
                     PATYTALDGVSAADVLDRLQFPEAARHLALEVFARSFFADPREFSGAELVAMFHMYFV
                     GSAEGLLFDVPADDYDSTLWGPLGAHLHSLGVAVHTGRSVESVTDSAEGVSVLHTAAA
                     GGGDAHWEQADAVVLALDPIGLRRVVDSSPGLGGDDPAGVAWRESVAGIRSAPAFAVW
                     RLWFSDLVAPERAPFLGTSGYGPLDNIS"
BASE COUNT         1897 a         5064 c         4473 g         1714 t
ORIGIN      
        1 acgtaccgtg acggcgtgag cgaccaggtc acaccgctcg acgcgatctt cctcatgggg
       61 gagacccccg agaccctgat gcacgtcgca tcgctgctgc acttcaccta cccagagggc
      121 gtgcggcgca ccacgttcct gcgccagctc gtgaacgacc tgcgggccgc ccccatcgag
      181 tcgccgtggt tctaccgcct gcagacccgc accctgatgc gccaacccgt gcaccgctgg
      241 gtcgaggact ccgagttcga catcgactac cacgtccgcc actcggccct gccgagcccc
      301 ggcggcgagc gcgaactcgg tgtcctggtg tcgcgactgc actccaacca gctcgacttc
      361 cgtcgccccc cgtgggagat gcacgtcatc gagggcctgt cgggcaaccg gttcgcgatc
      421 tataccaaga ttcaccattc gctcgtcgac ggcttcaccg gcatgcgcat cctccagcgc
      481 gggctggcca ccacccctga cgacctcgag caccccttct tcttcagcac ccgcaagcca
      541 tcgcgacccg agggcgcccg ccgccaggcc gacccggtgg gagacgtcac ctccatcctg
      601 cgctcgttgg cctcgtcggt cggcaccacg ccatcggtgc tgaaggcgct cgtggagacg
      661 caggtcgggc ggggccgcac cccgaccaag gccgtgacga gttaccaggc acccgactcg
      721 atcctcaact cccgcaccgg caggtcgcgg cgcttcgcga cccaggagta cgacctcggt
      781 cgactgcgag ccatcgccaa ggcccgcaag gccaccctca acgacgtgct catggcgatc
      841 tgcgccggtg ggctgcgacg gtggctcgac gacctcgagg cactgccgga gcgtccgctc
      901 atcgcgttca tccccgtcaa cgtccgtccc ccggagagcg agggtggcgg caacgccgtc
      961 ggcgcaaccc tggtctcgct ggcgaccgac atcgaggacc ccctggagcg gctgagcgcc
     1021 atcacggcat cgtcacgggc ggccaaggcc cggatgcgcg gcatgagcgc cgatgcagtc
     1081 atggcctaca gcgcgctgct gctcgccccc gccgggcttc aggtcgccaa agccatgagc
     1141 ggtctgcccg gcccggtgcc ccacaccctc aacgtctgca tctcgaacgt ccccggcccc
     1201 aaggaacacc tctacctgcg cggtgcgcga ctcgaggcga cctaccccgt gtcgatcccg
     1261 ggccactcga tggcgttgaa catcaccgcg cactcgtacg cgggcacgct cgacctcggc
     1321 ttcatcggcg accgcgaggc gctgccacac ctccagcgcc tagccgtacg cgccggtgag
     1381 gcgctcgagg aactcgaggc cgccgtggcc gggctcgact gactcaggac ccccttccag
     1441 ccaccgcgtg acgggctcag ccctgctgct ccccggcgat gttgaccatc catgcgacgc
     1501 cgaacttgtc gacgagcatg ccgtagtcgt caccccacat ctggcgctcc aacggcatct
     1561 gcacggtgcc gccttcggcg agcccgtccc agtagcggcg catggcctcg gagtcgtcgc
     1621 cgctgatcga catcgtgatg gtgttgccag ctacgtagct gtcctcctgg cccgggggca
     1681 tgtcggaggc catgaaggtc atgccggcct cggtctcgag gtgcgagtgc atgaccccgt
     1741 tcgcctcggg cccctgcgca ccgaagtcac cgaaggtgga gatctgcaac tcaccgccga
     1801 gcacggactg gtagaaggtc atcgcctcgc gcgtggttcc ggggaagttc aggtaagggt
     1861 tcagggcaat cgccatgacg ggctccttcg acgtcgaact ggatcggacc cgctcgggcc
     1921 cgccttcagc ggacgctaca accgggtgcc gacagacggg ctggattcct tcctggacct
     1981 gccctgcaca gccaccgtga ccgcgaggac cagcacagtg accatggcca gcatcatcga
     2041 cgggatcgtg acgttcgccg cgtggaagtg cggacccgac acccagacgt cggccgtgtc
     2101 gcggttgacg aagccgtcgt ccgcgaagcg cgcgagcgcc gtcgcgagct cggcgaggtg
     2161 cccgagcacg gcgagcggga cgaacagcag gagggccttg cagatgcgac gctgagccgt
     2221 ggactcggtc tgggcaagca gccgacgcac gagcacggcg gcgccgacga gcatggggat
     2281 ggccaggacg tggaacgcga tgtgcggata gctcgtgtcg ccgaaggcgg cgaatgcagg
     2341 aagcatgagg aagggcagcc agatgagcgc gatgagcatc aggtgtcgac gggtcacggg
     2401 ggaacctccg ggctgggggt ggacccttcg acgctaggaa aggcaccgcg ccacggcatc
     2461 ggcgaaaccg cctaactcgc gcggatcccc tcccgatggg cgtcgtgcca cgtgaggacg
     2521 gcgtgcacgc ggcggttgtc atgcggcgac tcgggcaggg ccagcttgct gaagaccgac
     2581 cgcaggtgcg cgtcgaccgt ccgctccgag agcaccagtc tggccgcgat acccgcgttg
     2641 gacagtccct cggcgagcag ggcgagcacc tcgcgctccc gtggcgacag caccggcagc
     2701 ggctgtgact cggatgggcc cgggaccggc ggcctcccgt cgatcgcatc gctgggctcg
     2761 ggctcgggct cgtcgagagc gccgtccagc gccagcggga ccaaggctgg cgggtcccac
     2821 ggtggcgggt ccagtgacga acggtccagt gaggaccggt ccagagcagg cgagaccaac
     2881 gccctggcca cccgcgactc gagggggcgg gtccccacca tgacggcgac ggccagcagg
     2941 gccgcgagca cgctgccgag cgtgccatcg gcgtccacca acaccacggc agtgactgcg
     3001 acgagggccg cgatgagcgc caccagcacc accgccgtcc aggagatggt gcgggtccgc
     3061 agggcgagcg ggctccgcag cgcccgcgac gtccctgccg acaccgtcgc gagggcggcg
     3121 tacatgccac acaggaccat caccgccggc gcctcggccc ccatcggacc ggccgcgagg
     3181 gcgccgagca ctccgcagcc gagcacgagc accgggccgc tgagggcggc gagcgccacg
     3241 aggaccagcc gcggtcgcgc ttcgcccacg gcatcccggg ctgcccgcgt cgcgaccacg
     3301 ggaccgacga gggtggaggc aaggaagacg aggttcacca cgaggcccac cgcgaccgcc
     3361 gtgtcggact ccacgagggg cgtgagaccg gccggccaca tgggtggggc caacagggcg
     3421 agcgccacgg catccacggt ggctagcgcg aggaccaccg taatcaggcg tggcggcagg
     3481 ccgttgccga tgtagcggac cgcgagcagc ggcagcaggg acagggccgc gatcaccggc
     3541 acgtgcccga ccgtggccag ggcagccaga agccggatcc acgaaccacc ggtggagtcg
     3601 ctcgcgacag ccagcgcgag cgtggcggcc acggccgggt aggcgagcgc gacgccgacc
     3661 aggccgagcg ttgcccgccg ccaggccacc caccccgggt cgaggccacg gtgggcgagc
     3721 agcaggacca ccgcggtcac gagggcccac accagcaccc agggggaagc acccggcggt
     3781 ccgggttcgg caacgacccc tgccgtcacg gtgaggccgg ccacggcgat catcaccgtg
     3841 gcagccgtga cctccacggg cgtcggtgac gtccggcgcg tcatccatcc agtgtcaggg
     3901 aagggacccc gacgtcgcca gtgcccgaac gggtcggacc tcggtggggc atgcccaccc
     3961 ggttgtgaca tgcagagcgc gcggaatgtc gcgcccactg catgtcacga gccgtcaact
     4021 acaacctggc gatgaccgcg cgaccgcgat cggtcagcga atcccgcttc acggcatcca
     4081 tgccgtccca cgtacgcgtg accatccgcg agcgcgggtt gccctcgaag acctcccggt
     4141 ggtcctcgac gaaggtccag tacagggcgt cccagtcctc gcgccacggg ccggggccat
     4201 ggtcggtcat ccgcctcagg taggcgctgc ccgagacgta cggcttggtc gtgaccgccg
     4261 ccccggccgc gaactgcgac atcgcgtgga cgttgggcac catgacccag tcgtaggcgt
     4321 cgacgaacat cgccatgaac cactcgtaca cctcgaccgg gtcggtgcgc agcaggcaca
     4381 tcgcgttgcc gagcaccatg agccgctcga tgtggtgcgc ccagccgcgc tcgagcaccc
     4441 cctcgatcgc gtggtccacg gggtcgagcc cggtcgtgcc gccccaccag cccggcgcca
     4501 aggaccgggt gtggcccagg tggttcgcga ccctcatccg gcgcccgaac aggtggtagg
     4561 tgccccgcat gtactcgcgc cacccgatga gctggcgcac gaagccctcg agcgacgcga
     4621 gctcgacccc ctgctcggcc gcgccggtga gcaccgcgtc gagcacctcg cgcgggtcga
     4681 gcaggccgca gttcagcgcc ggggtgatcg cggcgtggaa gagcaccggg tgctgcgtgg
     4741 agacggcgtc ctcgtacggg ccgaactgcg ccaaccgttc gtcgacgaac tcctgcaggg
     4801 ccgcccgggc ctgctcgtgg gtcaccggcc acgcgaagga tgccgcgcgg ccaggtgccg
     4861 cggggaactc gccctgcacc cacgagatcg cctcgtcgac ctcggcggtg cgctcggcac
     4921 cgctccagct gggcaccacg tggccgcggg gcagcttctt gcggttctcg gtgtcgaagg
     4981 accaccgccc accgacgggc tgcccgtcct cgaccagcac gtcgagccgg cggcgctgcc
     5041 actggtagaa ggtctgcatc cgggcccgcc gatcgccgaa ccagtcgacg agatcgggcc
     5101 tcgtggtgag gaagttcggc gactcgagca cgtcgtgcgg cccgaagccc accccggcgt
     5161 cggccacgga acggcgcagg tcacgcagca tccagtcgtc aaccgggtcg acgacgctga
     5221 gcgcatcagg tcgcagcccg gcgaggtggg cggcgagacg cccgcccgag ggccgcacgg
     5281 catccgtctc caggacgtcg acgtcgaaac ccgcgtcgcg caaccgctcc acgaagtggc
     5341 gagatgacgc ccggtgcagc acgagcttct gagcgtggaa cggctggtga cgaaagagca
     5401 ggtcgtcctc gacgagcacg aaacgcgttc cgggcggggc gtcgaggtgg gccgcgaaga
     5461 gctggtgcgg gtacacgacc cgcaccatgc gccgggcgac cccggacggc gcaccggccg
     5521 tcaccggcgg tgcgctcacg tgccctgaag gtcggcgagc accgtgcggg cgacccggtc
     5581 gccggagacc agggccccct ggatggaggc ggtgtcccgg tggtcgccag cgacgtagac
     5641 ccccggcgag acccgcgccc ggctgaccgt gcgcagcggt gcgggctggg tcggcagcgc
     5701 gtgcaggatg tcgtcgcggc gcagcagacc ccagtcggcg ggcgaggcac cgaacatctc
     5761 ccctgcctgt cgccgcacct cggcctcggt gccgccggtg ccgtccggcg ccagcaggca
     5821 ggtcacctgg acgaggtgtc gtccggccgg ggcgtacgac ggcgccgcgt tgctcatcac
     5881 cgcggcattg acgatgggcc cccgccgacg gccgtcgacc atgagcaggg tggatgccgt
     5941 cggcgcctgc ggggcgtcga accaccaggt ctgcaacccc ttggtcgcgg gggccggcag
     6001 cggcagcagc cccgccacgg catccggccc gacggcgacg acggcggcgc gggcactgac
     6061 ccgtgaaccg ccggcgagcg tgagcaccgt gcgcccggca tccgcgctga gcgaggtgac
     6121 gcgttcgccg agtcgtacgt ccacgcccgc cgcgcgcgct cgcccggcga gctgttccgg
     6181 cagggcccgg atgccgttcg cgggcacccc cggcacgccg agcgcgaaca tgcgaaccaa
     6241 caggcgtgcg aacgcctcgg aggtctcgcc ccggtcctcg gcgagcaccc cggcgaggaa
     6301 gggctcgatc acctcggtgc gcagcggacc gcgcaccccg gcgcggtcga gcccttcgcg
     6361 cagtgaccgg tcggcggcca aggccccggc gatgacccgg cggggcgcga ccacggccgg
     6421 cccggcccac cgggccagcg cggcgacggc gcgcggggag agcaggccgc tacgcagcgt
     6481 ggcggcgaga ccaccgggcg cccgcaacgg gtgggtgagt tcgacgaggc cacgctcacg
     6541 tcgcacctgc agaccggccc cgaaggggcg caggacgagg gcctcgaggt cgacccagcg
     6601 ctgcacggcg ggataggcgg gattgagcac ctggaagccg cggtcgagga ggaagccgtc
     6661 gacctcgtcg gtgcgttggc gcccacccac ggcatgcgcc gtctccagca tgaccacggc
     6721 ccgaccggcc tgcgcgagca cgtgcgcaca cctcagccca gccagcccgg cacccacgac
     6781 cacgacatcc gcatccacgg ccgcaaccct aggctgagca cacccgaacc acgtctcact
     6841 tgacaatact ttgtctaagg ttgtggacca ctccggggcc gttgtctagg ctttgcgcac
     6901 agcggtcagc cgccccggga cacatcccgt ccgaccagac cagcgaggca ggaggcgcac
     6961 gtcgatgagt gccgagacga ccgcactgaa gctcgatgcg ccggagacca cggccagcgt
     7021 cgaatccacc ctcacgcgcg ccgactccct gctcgagacg accttcctcg aactcgcgag
     7081 cgcgctcgac caagccgcgg ccgacaacgc ccccgacgag gcgagcccga ctgccggccc
     7141 gctcggggtc gacctggtcg gtgagctggc cgagcgnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnncgag cccgactgcc ggcccgctcg gggtcgacct ggtcggtgag
     7321 ctggccgagc gctcgcgcaa ccacggcaag cgcctgcgcc cgaccctggc ccactggggc
     7381 tgggtggtcg gcggcgccga gcccggcagc cacaccgacc tcgtgcggat cgcggcggcg
     7441 ctcgagctgc tgcacctgtt cgcgctgatc caggacgacg tcatggatcg ctccgactcg
     7501 cggcgtggtc gccccacgct gcacgtcgtg gcggcgcagc gccaccgtga cgagcagggc
     7561 ctcggcgacg acaccctctt cggcgacagc gtggccaccc tcgtctccga cctggcgctg
     7621 agcgaggcca cgctgctcgt cgcccccacc gccgccaccg tgcgggccgc ctggcgactc
     7681 atggcggtcg agctcgtcga gggccagctg ctcgacgtca cccacaccgc gggccgccgc
     7741 cgcgaccacg cgacctcgcg ccggatcgcc cggttcaaga gcggccgcta cacgatcacc
     7801 cgtcccctcc agctcggcgc actggtgggt ggggccgacg agcacaccgt gcgcgggctc
     7861 atcgcgtggg gtgacctcat cggggacgcc ttcgccgtgc gcgacgacgt cctcggcgtc
     7921 tggggcgacc cgacccagac cggcaaaccc gccggtgacg acctgcggtc gggcaagccc
     7981 accgagctgc tcacctgggc cgccgagctc cttcccccga gcgaccgtcc cctgttggac
     8041 gcctgcgacg ccggcaccct cgacgacgcc ggggtcgacg ccctccagca ggcgatggtc
     8101 gctgccgggg ttcgcgaccg ggccgagcag gccgtcgccg acctcagccg tcgcgcccac
     8161 gcacgcctcg acgccctcgc cgtcgaaccc cgggcggatg ccgcgctgcg cggcctcgcc
     8221 gacgccatcg cctggaggtg ctcatgaagg ttctcgtcgt cggggccggc ctctccgggc
     8281 tggccgccgc ctgccacctg accgccgcag ggcacgacgt caccgtgctc gagcgcgagg
     8341 acgtcgtcgg tggccgcgcc ggcctgctgc gcctcggcga cttccgcctc gacccggggc
     8401 cggtcgtcat gaccatgccc gagctgctcc acgacccgat ccgggccgtc ggcggcgacc
     8461 ccgactcact gatcaccatg cgtcacctcg accccgccta ccgcgcggtc taccccgacg
     8521 gcagcgagct gcacatccgc gccgacatgg acgacctgcg cgaggagatc cgcaccaagg
     8581 tcggcccggc cgacgccgcg ggcttcgacc gtttcctggc gtggctggag cagctctacg
     8641 acaccgagtt cgacaccttc gtcgaccaca actacacctc accgctcgac ctcgtgcgct
     8701 ccccggcgac cgcactcaag ctgttgcggc tcggcggcct cggcgccctc ggcccgtccg
     8761 tcgaccggtt cttcaccgac gaccggctca cccgcatctt cagcttccag gccctgtacg
     8821 ccggcatctc gcccgccaag gcccgcgcgg tgctcgccgt catcacctac atggacaccg
     8881 tgcgcggcgt gttctacccc gagggtggca tgcacgccgt gccccgggcc atggcccagg
     8941 ccctcaccga cgcgggggtc ccgatccacc tcgacgtcga ggtcagcgag atcctgcgcc
     9001 gcggcgacgg agccgtcgcc ggggtcgcca ccaccggcgg tgagcgcctc gcggccgacg
     9061 ccgtggtcgc gaccgtcgac ctcccggtgg tctacgaccg cctgctcccc ggcatccgcg
     9121 ccccccgcac catcaggacc ggcagctact ccccctccgc cgtcgtgtgg cacgtcggcg
     9181 cctcgggcac gcccggcccg cacctgcgcc accacaacat ccacttcggc accgactggg
     9241 acggtgcgtt cgaggcgatc atcgaccgcg gcgagctcat gcccgacccg tcgcgcctgg
     9301 tcaccgtgcc ctcggtgacc gacccgaccg ccgcaccggc cggctccacc gccatgtacg
     9361 tgctcgaacc ggtgccgcac accggtgccg ggctggactg gcgccgtgag gccgggccga
     9421 tgcgtgagcg gctcctgcgc ttcctcgagg acggcggcta cccgaccgac atccgcgaag
     9481 aggccttcat cacccccgac gactggcgcg cccagggcat gcacctcggc accccgttcg
     9541 ccctcgccca caccttccgc cagtccggcc ccttccgccc ctcgaacgtc gaccgccggg
     9601 tgcccggcct ggtgttcgcc ggctccggca cccagccggg cgtcggcatc cccatggtgc
     9661 tcgtcagcgg caagctcgcc gcgcagcgtg tccagcagta cgccgggggc gcgcgtccgg
     9721 tcgccaggac accacgtacg gtggcccggt gaccgaaccc accccggcca cctcacccga
     9781 ggtgctggcc gccggctacg cccgctgcgc cgagatcacc cgtgagcacg ggacgacgta
     9841 ctactggggc gcccggctcc tgcccgccgc ctcgcgccgc cacgtgcacg ccgtctacac
     9901 cctggcccgc ctcgccgacg acatcgtcga cctcgcgggc cccgaacccg gcccggagac
     9961 cgccgcggag ctcgacgcct tcgaggcgtc gttctggcag gcgctcgccg acggcaccta
    10021 caccgacccc gtcatggccg ccgtcgcgac gtcggtgcgc gagtgcggca tcgccgacga
    10081 gtgcttcacc cgcttcttcg gtgccatgcg cagcgacctg acccagcgca cctacgagac
    10141 ctgggctgac ctgctcgcct acatggacgg cagcgccgct gtcatcggcg agatgatgct
    10201 gcccgtgctc aaccccacga cggatgccgt cgtgccggct cgcgcgctcg gcctcgcctt
    10261 ccagctgacg aacttcctgc gcgacgtcgg cgaggacctc gaccgcgggc gcgtctacat
    10321 cccccaggag gacctgcgcc ggttcggcgc cgacccccac gcccgcgtcg tcaccccgca
    10381 gtggcgcgag ctgatggcct tccagatcga gcgcaaccgc cgcctctacc gcgaggccga
    10441 cgccggcatc cccatcctgc ccggggcatc ccgccgctgc gtcgccacgg cacgggtgct
    10501 ctacgcccgc atcctcgaag gcatcgaggc cgccgactac gacgtgttca cctctcgcgc
    10561 acgagtcccc accgcgcgca aggccgcgct cgcggcgcgc atggtgacct cgcgcgaccc
    10621 gatgcgactc gtggcccgcg accgcgccgc cgtcgacccc ttcggcgggg cctgggtgtc
    10681 ggatgacccc gtcgtgctgg tcgacgaggg cggcgccccg atcggtcagg gccgcaagtc
    10741 cgcgatccac cacgacgaca cgccgctgca cctggcgttc tcctgctacg tcctggaccc
    10801 ggcaggtcgg ctgctcgtga cgacgcgggc ggcgagcaag ccgagcttcc ccggggtcgt
    10861 caccaacacc gtgtgcggcc acccgacccc cggcgaggac ctcgcgcagg ccgtgcgacg
    10921 gcgggcccgc agcgagctgg gcgtcgaggt gggcgacctg cgtctcgtgc tgcccgagtt
    10981 ccgctaccgg gcgcagatga acggcatcgt cgagaacgag gtgtgcccgg tgttcgtcgg
    11041 gtgggcgacc agcgactcca ccgcccccga ccccgaagag gtcgacgacg tgcagtgggt
    11101 gccctgggag cagttccgtg acgaggtgct cgagggagtg cgcaccgtct cgccctgggc
    11161 caccgagcag gttccgttgc tggccgcgct cggccccgac ccccgctcct ggcccgccgg
    11221 gtcccgcgcg gcactgcccc cggcggctac cctcggcccc ggcgccgacg cactggcccc
    11281 ggcgtcctga gcgccgcgac cgttgaaccc ccgagccgcc gacacccacg tcgaggacac
    11341 catcaaggag accgtgtgag cacgtccgca ccgagcacgc agggctgggt ccttgccgac
    11401 gccttcgacc gggccgcccc cacgtacgac gccatggtgg ccctgtcccc cggctaccac
    11461 gaccagctgc tcggagccgc cggcgcgctg ctcgaccggc tgcccgcccc ggacggctcg
    11521 gccctgaagg tgctcgacct cgggtgcggg tcaggcgcct cgacacaggc cctgctcgag
    11581 cagtggcgca cccgcggggg cgccgccgac ctgctgagcg tcaccggcgt cgacatgtcc
    11641 gaggggatgg tcgcgcaggc ccgcgccaag tcctgggcca ccggggtcga cctcgtcgtc
    11701 tcggatgccg tgacctacct cgaggcgctc ccggacggct cggtcgacgg catcctcgcc
    11761 tgctacctgc tgcgcaacgt gcccgaccgc gagcgcctcg tgcgcgagat ggcccgcgtc
    11821 ctgcgcccgg gtggcggcgt cgtcatccac gactactccg tgcagggcag cacccgcgcc
    11881 aaggccatct gggcgacgat ctgccacggc gtcatcatcc cgctggccgt catcaagcgt
    11941 tccgacgtgc ccctgcaccg ctacctctac acgagcgtgc gcgacttcga ctcggtcgcc
    12001 cggatctgcg agcggctgct cggcgccggc atggtcgacg tgcggcaccg cacctacccc
    12061 ggctggcagc gcgacgtcat ccacaccgtg ggcggatcgg caccgtcgtg aacccgctac
    12121 gccgcacccc gacgtggcgg ccgggacggg acgcgcgagc cgtgttccac ccgccgccgg
    12181 cacccacgac ccccgtggat gccgtacccg cctccgcgcg ccacgtcgtg gtcgtcggtg
    12241 ggggcatcgc ggggttgacc tccgccctcg cgctcgcgga gcgaggggtg cgggtcaccg
    12301 tgctcgagcg cgatcagcgc ctcggcggcc gcgtgcgtgc gtggccggtc gacgggcccg
    12361 ggagcgacgc ctggatgagc cggggtttcc acgccttctt ccggcagtac tacaacctgc
    12421 gcgccgtgct gcgccgcacc gaccccacgt tgtcgcggct tcgccccctg gacgactacc
    12481 cgctggtgcg cgatggcggg ggtcaggact ccttcgcgaa gatcccgcgc acacccccgt
    12541 ggaacctcgc tgccttcgtc gcccgcagcc ccagtttcga cctgcgcggg ttggccggtg
    12601 tcgacgtcga ggccgccctc ggcctgctcg acgtcgactt cccggcgacc tacacggcgc
    12661 tcgacggggt cagcgccgcc gacgtgctcg atcggctgca gttccccgag gccgcgcgcc
    12721 acctcgccct cgaggtcttc gcccgcagct tcttcgccga cccgcgcgag ttctccggcg
    12781 ccgaactcgt cgcgatgttc cacatgtact tcgtcggatc ggccgagggg ctgctcttcg
    12841 acgtaccggc cgacgactac gacagcacgc tgtggggccc gctcggcgcc cacctgcact
    12901 cgctcggggt cgccgtgcac accgggcggt cggtcgaatc ggtgaccgac agcgccgagg
    12961 gggtgtccgt gctgcacacc gctgccgctg gtggcgggga cgcccactgg gagcaggcgg
    13021 atgccgtggt cctggccctg gacccgatcg gcctgcggcg ggtcgtcgac tcctcccccg
    13081 ggttgggggg cgacgacccg gccggcgtcg cgtggcgcga gtccgtggcc ggcatccggt
    13141 ctgctccggc gttcgccgtc tggcggctgt ggttctccga cctcgtggcg cccgagcggg
    13201 caccgttcct cgggacgagc ggctacgggc ccctcgacaa catctcgg
//
LOCUS       DAOSOC010000050        13158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4740_length_13158_cov_9.581928, whole genome shotgun sequence.
ACCESSION   DAOSOC010000050 DAOSOC010000000
VERSION     DAOSOC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4740_length_13158_cov_9.581928"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PL014_05705"
     CDS             complement(<1..421)
                     /locus_tag="PL014_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT56621.1"
                     /translation="MLQTTKQIFVTGGVASSLGKGLTASSLGHLLRARGLRVTMQKLD
                     PYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDVALKGSANVTTGQVYNRVI
                     QRERRGEYLGDTVQVIPHITNELKERMRAQAAGSPGVS"
     gene            complement(510..1610)
                     /locus_tag="PL014_05710"
     CDS             complement(510..1610)
                     /locus_tag="PL014_05710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT56622.1"
                     /translation="MKVLMVLGTSAGGVGAHVKGLIGSLTGQGHTVVVACPPEVEEHF
                     HFARVATTTASLEVADRPHPTRDARAVRDLARLSAKADVVHAHGLRAAALAVIATARS
                     GVPVVATLHNAAPSGALTSAVYGGLERVVARGSDLVLGVSADLVERMRRLGARRTGLA
                     VIAAPPRREAERDRYAVRSELGIGGRTSLGVVVARLAPQKGLPLLLDAHHELSTQGLD
                     LLTVIAGDGPLRESLQARIDAENLPVRLLGHRSDVPDLLRAADVVISSAVWEGQPVGI
                     QEALHAGAAVVATDVGGTAAVTGGAALLVPPDDPLSLSRAIRDVVLHGSVRDDLRSKA
                     VERSGELPTDADAVAAALGAYASVLGQGPDGR"
     gene            complement(1607..3238)
                     /locus_tag="PL014_05715"
     CDS             complement(1607..3238)
                     /locus_tag="PL014_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOT56623.1"
                     /translation="MTGRRFASGIAGAAGLIAVTTLLARVVGFGRILVFADAVRAGGV
                     GGIYSSVNALPNVMFEVAAGGVLAAVAVPLIAHRLGAGERDRADQTASVLLSWAMLVL
                     LPVAALLWLLAGPIAGWLIRSPDPAAEDVAATLLRIFSVQVPLYGLGIILTGLLQAHR
                     RFLAAALAPLVSSVVVLVSYLWYGSIVDGETAPSLVSAEAIRVLGWGTTMGVVVLSVP
                     LVVPALRTGWRWHPGLRLPSDDARRLRVLAGAGMLALLAQQGAVLVTMWLANHTGDDG
                     TFTVYLYLQAVYLLPYAVLAVPVATSAFPALATGTGAGQDVSGTLARSLRAVLVLTGL
                     SVGVLIAAAPAIGPFFAILDARRSDGVASTAALAALPGALNAYAPGLVGFGLIALLTR
                     ALYVRGKPMDAALAAAIGWSIAALLPLAVLGSGQDPGLTLRWLGLSSTIGMTVAAVAL
                     LLLVRRTWGPAVTQGIGRTTGALVVSVALAVAAGDVVTRGRALDDLVETMVVGAGAGL
                     LALMIGVVALMVGDRAMMSEVLTRGRARRRGRGQE"
     gene            complement(3235..4050)
                     /locus_tag="PL014_05720"
     CDS             complement(3235..4050)
                     /locus_tag="PL014_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56624.1"
                     /translation="MIARTAAIGGLAAGAAHGAFRFLTSAVTSRRWARTNHAGHDVTL
                     LEGPAYVVGATVGAAAGALTAGPAGVVAAIGSGAFGALDDLGGDGSSKGLKGHLGAAA
                     RGQITTGFIKIVGIGATGVAAAILADRSRHDVDAVDALVGGAVIAGSANLANLLDLRP
                     GRALKVTLLAALPLMVRRGPGASAATAAAGAALGVIGPDLAGTAMLGDTGANAAGALV
                     GTALVEATGRRGRVAALVVLAGLTLASERVSFTKVIESTPGLREFDAWGRPAR"
     gene            complement(4047..5006)
                     /locus_tag="PL014_05725"
     CDS             complement(4047..5006)
                     /locus_tag="PL014_05725"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HOT56625.1"
                     /translation="MIDFRYHLVSIVSIFLALAVGIVLGAGPLQGELGDTLTNEVAGL
                     REDKAQLNAQLSDATAEIDAREDYIGETNPAILADSLDGQRVAVVVLPGTDTALVEST
                     ATSLASSGAAVVSTTTIGQNWVSTDEAVVTARDAAVRDAAAGAGIDVSDTGSVSARDV
                     LLAALLSTSARTLPNTIDAQQARSALATLASADLIEVAGEDVQIADLVVVVAGTITEG
                     NPAAQQAAADRWVDLVIALDARSAGLVTAGATTTATDAIDVVASIRNDATATEGVSTV
                     DNGGSTLGQASIVHALVEQRAGEVGQYGFAEGADAPFAPVPAP"
     gene            complement(5007..6182)
                     /gene="steA"
                     /locus_tag="PL014_05730"
     CDS             complement(5007..6182)
                     /gene="steA"
                     /locus_tag="PL014_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HOT56626.1"
                     /translation="MSIRLPSRRGRGPLPGLTGPARVDRRTKNLTKRLRPGDIAVIDH
                     EDLDRVSAEALVACRPAAVVNAAASISGKYPNLGPEILVAAGIPLIDSAGPDAMQIKD
                     GVLLTIDGGDLIADERTIVTGERLDAEAVQAMMAVARAGMSDQLEAFAENTMDYLRQE
                     RDLLFDGVGVPDITTELEGRHVLIVVRGYHYKEDLQILRHYIREYKPILIGVDGGADA
                     LIEARHKPDLIVGDMDSVSDDALRSGAEVVVHAYRDGKAPGLERVRSLGVEPVVFPAT
                     GTSEDVAMLLADDKGAQLIVAVGTHVTLVEFLDKGRKGMASTFLTRLRVGSKLVDAKG
                     VSQLYRSRISLWSLFLMLAVGLLALAAALWSTDGGRALIQVFGARLDDLWSSIQGVF"
     gene            complement(6206..7897)
                     /gene="recN"
                     /locus_tag="PL014_05735"
     CDS             complement(6206..7897)
                     /gene="recN"
                     /locus_tag="PL014_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT56627.1"
                     /translation="MIEEIRIRDLGVIDEAVLPLHPGLTVLTGETGAGKTMVVTGLGL
                     LLGGRGDSALVRAGADRVVVEGVLSLPEGHRALGRAADAGADVRDGLVLVRSVASDGR
                     SRAHVGGRAAPVGLLAELAEHLVAVHGQADQWRLRRADQHREVLDGFGGGDVATALTD
                     YRAVHGELIGAEAELGDLRAAAAARAREAETLRVGLERIEALDPQPGEDDALRVESQR
                     LGHAEELRDGAGAAHDALTGGDDAGGPANPGVLGALATASARLDQVSASDPALAEIGT
                     RVDELRYLAADVAADLGSYLADADIDPARLEQVESRRAALSELTRLYGPGVDDVLAWA
                     RDAAVRVAELESTTDRIDELAARVEVLTAERERAAATLTARRTAAASTLGDRITEELT
                     HLAMGSATVTVDVADAGRFGAEGRDDVEIRLASGSGTAPRSVTKAASGGELSRVMLAL
                     EVATATETASVPTFVFDEVDAGVGGRAALDVGARLAALARHAQVIVVTHLAQVAAHAD
                     RHLVVERSDDGHVTRSGVHEVTGEDRVAELARMLGGSGTDAALEHARDLMARARG"
     gene            complement(7894..8841)
                     /locus_tag="PL014_05740"
     CDS             complement(7894..8841)
                     /locus_tag="PL014_05740"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOT56628.1"
                     /translation="MTSTPSRRILLVTHLWRTDAQELAAGVATRLIAAGVGVSASRDE
                     LVDTPLARVPGVLGVDTDTDESAAAGCELVCVLGGDGTILRGAELSRGSGVPLLGVNL
                     GHVGFLAEAEREDLDATVERIVAREYHVEERTTLEVRAEEDGRTVFQSWALNEVTVEK
                     ASRERMLELTVEIDGRPLSTWGCDGVVMATPTGSTAYAFSAGGPVVWPDVEALLLVPI
                     SAHALFARPVVVGPQSRFVVEVLAQTDAHGVVWCDGRRAVGLPPGARIHVRRSDEPVR
                     LARLNSGGFTDRLVEKFDLPVEGWRGAARRQLAQGDGSP"
     gene            complement(8844..9707)
                     /locus_tag="PL014_05745"
     CDS             complement(8844..9707)
                     /locus_tag="PL014_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOT56629.1"
                     /translation="MRPSTAPCRGGSPTSAADIARLDAELVRRGLARSRGEAQEFVRA
                     GQVTIGGSVATKPSLPVADDDVLVVEVAGPRWVGRAAHKLDAALQEWGPVGLTVKGRR
                     CVDVGASTGGFTQVLLAADAAHVAAVDVGRGQLVPDVASDPRVTEHSGTTIRGLQPET
                     IGGPADVVVADLSFISLTLVLADLARLMGERGDLVVLVKPQFEVGRGRLNRQGVVTDP
                     RERTAALQRVVESTLDAGLFVHGIRESPIAGTTGNIEYLLWARSDPSDTMSADRVRDI
                     VREITTEARGG"
     gene            complement(9655..9849)
                     /locus_tag="PL014_05750"
     CDS             complement(9655..9849)
                     /locus_tag="PL014_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56630.1"
                     /translation="MRLPDIDDAAQQDQQAGPPETGDIVIDAALRDLANTPAGDLDGQ
                     LQAGEAVHRTLQGRLADLGG"
     gene            9865..>10199
                     /locus_tag="PL014_05755"
     CDS             9865..>10199
                     /locus_tag="PL014_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56631.1"
                     /translation="MLPDAGADGACVAGESLDAVLFAAGADFVAAFFAGAFAAALAVA
                     DVVAAFFAGALAAGAFAAAFAGPDVVAVALLAGAFAAALAAGALAAAFAVVALAFFAG
                     AAFVAAFFAG"
     assembly_gap    10200..10299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10300..10805)
                     /locus_tag="PL014_05760"
     CDS             complement(<10300..10805)
                     /locus_tag="PL014_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56632.1"
                     /translation="MAKNSIQGYVELASGLGDLTRSRAKEAAQEIIALASQDTSRKKL
                     AKQASALADDLLKAAEANRKQIVRLVRREVDSAVGKLDVDRLTREVQGLGATVATLAS
                     QVEELARSAASVEIRGAATVVSDIVEVAPEPVKAAAKKAPARVAKKAPAKKATTKAAP
                     AKAAAKAPA"
     gene            complement(10855..11808)
                     /locus_tag="PL014_05765"
     CDS             complement(10855..11808)
                     /locus_tag="PL014_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOT56633.1"
                     /translation="MSGPQTLAARYAAIVCDLDGVVYRGPTAVPHAIEALTSSNLPVI
                     YATNNASRTPEDVAAHLRDLGLDCEPGDVATSSQAGARLVADNVPPSSRVLAVGGPGV
                     SAALVQVGLVPVVPADGLGHDVVAALQGYGAGVTASDLAEVAYAVQAGATWVATNTDA
                     TLPTNRGVAPGNGSLIGAVQRAVGHAPDMVAGKPSAPLYLMCADRLAIPAERVLAVGD
                     RLDTDIEGAVAAGMDSLLVLTGVDDLAACLEAPPHRRPTWVAPDLRALNVDPDTEGDW
                     LHALSRAVVAVHRARDAGSPASDIAALSESAESVLAAVRFR"
     gene            complement(11805..12524)
                     /locus_tag="PL014_05770"
     CDS             complement(11805..12524)
                     /locus_tag="PL014_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56634.1"
                     /translation="MTGKELDRAVHQQLRTLSKENAEGVAQHLVMVAATLEADNLEAA
                     LAHAETAVRRAGRVPAAREALGMVAYRLGDFARALTEFRTVRRLSGSSHLLPLMVDCE
                     RGLGRHSRALELAATPEARTLTEDERQELAIVVSGIRRDLGQDDAALVALQIPALRRA
                     AGPSVCRLHYAYADVLLGLGRDEEARAWFLKAVEGDVDAVTDAVERLEELDGIVVDLG
                     EDDEDDCAELSEVTAVAPEDV"
BASE COUNT         1845 a         4721 c         4705 g         1787 t
ORIGIN      
        1 ccgagacccc gggcgagccg gcggcctggg cgcgcatccg ctccttgagc tcgttggtga
       61 tgtgcgggat cacctggacg gtgtcgccga ggtactcccc gcgccgctcg cgctggatga
      121 cccggttgta gacctgaccg gtcgtgacgt tggcgctgcc cttgagcgcg acgtcgagga
      181 atcgctcgta gtggccgatg tcgaggtcgg tctcggcacc gtcgtcggtg acgaacacct
      241 caccgtgctg gaaggggttc atcgtcccgg ggtcgacgtt gagatagggg tcgagcttct
      301 gcatcgtcac ccgcagcccg cgcgcccgga ggaggtgacc gagggaggag gccgtgagcc
      361 ccttgccgag ggaggaggca actcctccgg tcacgaagat ctgtttcgtc gtctgaagca
      421 ccacgggatt caacggtacg tcatggcgcg ggggttgcgg gacggcttgg gtcggcgcgt
      481 cgcgaggtcc cacgtttcgg accggtggtt caccgtccgt cgggcccctg cccgagcact
      541 gacgcgtagg cgccgagcgc cgccgccacg gcatccgcat cggtgggcag ctcgccggag
      601 cgctcgacgg ccttggagcg caggtcatca cgcaccgatc cgtggagcac cacgtcacga
      661 atcgcgcgcg acagcgacag ggggtcatcc ggtggcacga ggagcgctgc accacccgtg
      721 accgcggccg ttcccccgac atccgtcgcg acgacggccg caccggcgtg cagggcttcc
      781 tggatgccga ccggttggcc ttcccagacc gcgctggaga tgacgacgtc ggcagctcgc
      841 agcaggtccg gcacgtcgct gcggtggccg agcaggcgca cggggaggtt ctcggcgtcg
      901 atgcgcgcct ggagggactc gcgcagcggc ccgtccccgg cgatgacggt cagcaggtcc
      961 agcccctggg tcgagagctc gtggtgggcg tccaggagca gcggcagccc cttctggggc
     1021 gccagtcgcg cgacgacgac cccgagggag gtgcgtccgc cgatcccgag ctcggatcga
     1081 acggcgtacc ggtcgcgctc ggcctctcgt cgcggcggtg ccgcgatgac ggcgaggccg
     1141 gtgcggcggg caccgaggcg ccgcatgcgc tcgacgaggt cggccgacac cccgagcacc
     1201 aggtcactcc cccgagcgac gacccgctcc agccccccgt agaccgccga cgtgagcgcc
     1261 cccgacggcg cggcgttgtg cagggtcgcg acgacgggaa cccccgagcg ggcggtggcg
     1321 atcaccgcga gggcggcagc ccgcagtccg tgcgcgtgca cgacgtcggc cttcgccgac
     1381 agtcgcgcca ggtcgcgcac ggctcgggcg tcgcgggtcg ggtgcggtcg gtcagcgacc
     1441 tcgagggatg ccgtggtcgt cgccacccgg gcgaagtgga agtgctcctc cacctctggc
     1501 ggacaggcga cgacgacggt gtgtccctgc ccagtgaggg acccgatgag ccccttgacg
     1561 tgcgcaccga ctcctccggc gctcgtcccg agcaccatga gaaccttcac tcctgccccc
     1621 ttccacggcg cctggccctc ccgcgcgtga ggacttccga catcatcgcg cggtccccga
     1681 ccatcagcgc gacgaccccg atcatcaggg ccagcaaccc cgcgcccgcc ccgacgacca
     1741 tggtctccac gaggtcgtcg agggccctcc cccgcgtcac gacgtcgcca gcggcgaccg
     1801 cgagcgcgac cgagacgacg agtgcccccg tcgttcgacc gatgccctgg gtcactgcag
     1861 gaccccacgt tcgccgcacc aggagcagta gtgccaccgc ggccacggtc atcccgatcg
     1921 tcgaggacag gcccagccac cgcagggtga gccccgggtc ctgcccgctg cccagcaccg
     1981 cgagcggcag cagcgcggcg atgctccagc cgatggcggc ggccagcgcg gcatccatgg
     2041 gtttgccgcg cacgtacaac gcccgcgtca gcagcgcgat gagaccgaaa cccacgagtc
     2101 cgggggcgta ggcattgagc gcgccgggca gggccgccag ggccgcggtg ctcgccacgc
     2161 cgtcactgcg gcgcgcgtcg aggatggcga agaaggggcc gatggccggg gccgcggcga
     2221 tgaggacgcc gacggagagc cccgtcagca cgagcacggc acgcagcgag cgggcgaggg
     2281 tcccgctgac gtcctgcccg gcgccggttc cggtggccag tgccgggaac gcgctcgtcg
     2341 cgacgggaac ggcgagcacg gcgtacggca gcaggtagac cgcctggagg tagagataca
     2401 cggtgaaggt gccgtcgtcg ccggtgtggt tggccagcca catcgtgacg agcacagccc
     2461 cctgctgtgc gagcagggcg agcatcccgg cgccggcgag cacgcggagg cgacgggcgt
     2521 cgtcgctggg caggcgcagt cccggatgcc agcgccaacc ggtgcgcagg gcgggaacga
     2581 ccagtggcac ggagagcacg acaacaccca tcgtcgtgcc ccaccccagc acgcgaatgg
     2641 cctcggcgct cacgagtgag ggtgccgtct cgccgtcgac gatcgagccg taccagaggt
     2701 aggacacgag gacgacgacc gaggacacca gaggcgccag cgccgcggcg agaaagcggc
     2761 ggtgggcctg gagcaacccg gtgaggatga tgcccagacc gtagagcggc acctgcacgg
     2821 agaagatccg cagcagggtc gcggcgacgt cctcggcggc cgggtcgggt gacctgatga
     2881 gccagccggc gatcggcccg gcgagcagcc agagcagggc cgcgaccggg aggaggacga
     2941 gcatcgccca cgagagcagg acggatgccg tctggtcggc ccggtcacgc tcccccgccc
     3001 cgagtcggtg ggcgatgagc ggcaccgcga cggcggccag cacaccaccg gcggccacct
     3061 cgaacatgac gttgggcagg gcgttgacgc tggagtagat gccgcccact ccgccggcgc
     3121 gcacggcatc cgcgaacacc aggatgcggc cgaagccgac gacacgggcg agcagggtgg
     3181 tgacggcgat cagcccggcg gccccggcga tgcctgaggc gaagcggcgt cccgtcatcg
     3241 cgccggccgg ccccaggcgt cgaactcacg caggccgggc gtcgactcga tgaccttggt
     3301 gaagctcacc cgctccgagg ccagggtcag gccagccagc accaccagtg cagccacccg
     3361 tccccgtcga ccggtggcct cgaccagagc cgttccgacc agtgccccgg cggcgttggc
     3421 gccggtgtcc ccgagcatgg ccgtccccgc cagatcggga ccgatcacgc cgagagccgc
     3481 ccccgcagca gcagttgcgg cggaggcgcc cggaccacga cgcaccatca gcggcagggc
     3541 ggcgagcagg gtcaccttca gagcgcgccc cggccgcagg tcgagcaggt tcgcgaggtt
     3601 ggcagatccg gcgatgacgg ctccaccgac gagagcgtcc acggcatcga cgtcgtggcg
     3661 cgaacggtcg gcgaggatcg ccgcagccac accggtggcc ccgatcccca cgatcttgat
     3721 gaagcccgtc gtgatctggc cacgggcggc agccccgagg tgccccttga ggcccttgct
     3781 cgagccgtcg ccgcccaggt cgtccaacgc gccgaacgca ccggagccga tggctgccac
     3841 cacgcccgca gggcccgccg tgagggcacc tgcggcggcc cccaccgttg ccccgacgac
     3901 gtaggccggg ccctcgagga gggtgacgtc gtgccccgca tggttcgtgc gcgcccaccg
     3961 gcgggacgtc accgcgctgg tgaggaaccg gaaggcaccg tgggccgccc ccgcggccag
     4021 gccgccgatg gcggcggtgc gagcgatcac ggcgccggga cgggagcgaa cggggcgtca
     4081 gcaccctcgg cgaagccgta ctgcccgacc tcgccagcac gctgctcgac cagggcgtgc
     4141 acgatgctgg cctggccgag cgtcgacccg ccgttgtcca ccgtggacac gccctcggtg
     4201 gccgtcgcgt cgttgcggat gctggccacc acgtcgatgg cgtccgtggc cgtggtcgtc
     4261 gcgccagcgg tgaccagacc tgccgatcgc gcgtccaggg cgatgacgag gtcgacccac
     4321 cggtcggcgg cggcctgctg ggcagcagga tttccctcgg tgatcgtccc ggcgaccacg
     4381 acgacgaggt cggcgatctg cacgtcctca ccagcgacct cgatgaggtc tgccgacgcg
     4441 agcgtggcca gggcagacct ggcctgctgt gcgtcgatgg tgttgggcag ggtgcgggcg
     4501 gatgtgctga gcagtgctgc gagcagcacg tccctcgccg acaccgaccc ggtgtcgctg
     4561 acgtcgatgc cggcaccggc agcggcgtca cgcaccgcgg cgtcgcgagc cgtcacgacc
     4621 gcctcgtcgg tgctgaccca gttctggccg atggtggtgg tcgagacgac agccgcgccg
     4681 gaggaggcca ggctcgtcgc ggtggactcg acgagcgcgg tgtccgtgcc gggcagcacg
     4741 acgacggcca cccgctggcc gtccaggctg tcggccagga tggcggggtt ggtctcaccg
     4801 atgtagtcct cgcgggcatc gatctcggcg gtcgcgtcgg acagttgtgc gttgagctgg
     4861 gccttgtcct cccgcaggcc ggcgacctcg ttggtcaagg tgtccccgag ctcgccctgg
     4921 agcgggccgg caccgagcac gatccccacc gcgagcgcga ggaagatcga gacgatggag
     4981 acgaggtggt agcggaagtc gatcacctag aaaactcctt ggatggacga ccagaggtcg
     5041 tcgaggcgtg caccgaagac ctggatgagc gcgcggccgc cgtcggtgga ccacagcgcg
     5101 gctgcgagcg cgagcaggcc cacggccagc atgaggaaaa ggctccagag ggagatccgg
     5161 gaccggtaga gctggctgac ccccttggcg tcgacgagct tgctccccac ccgcagccgg
     5221 gtgaggaagg tgctggccat gcccttgcgg cccttgtcga ggaactccac gagcgtgacg
     5281 tgggtgccga cggcgacgat gagctgggcg cccttgtcgt cggcgagcag catggcgacg
     5341 tcctcgctgg tcccggtcgc cgggaagacc accggctcga cgccgagcga gcgcacccgc
     5401 tcgagcccgg gcgccttgcc gtcgcggtag gcgtgcacca cgacctcggc gccgctgcgc
     5461 agggcgtcgt cggacaccga gtccatgtcg ccgacgatga ggtcgggctt gtgcctggcc
     5521 tcgatgaggg cgtcggcacc gccgtcgacc ccgatgagga tcggcttgta ctcacggatg
     5581 tagtggcgaa ggatctgcag atcctccttg tagtggtagc cgcgcacgac gatgaggacg
     5641 tgccggccct cgagctcggt ggtgatgtcg gggaccccga cgccgtcgaa gagcaggtca
     5701 cgctcctggc gcagatagtc catcgtgttc tcggcgaagg cctcgagctg gtcggacatc
     5761 ccggcgcggg cgacggccat catcgcctgg acggcttctg cgtcgagccg ctccccggtg
     5821 acgatggtgc gctcgtcggc gatcaggtcg ccaccatcga tggtgagcag caccccgtcc
     5881 ttgatctgca tcgcgtccgg gccggcgctg tcgatcaacg ggatgccggc cgccacgagg
     5941 atctccgggc cgaggttggg gtacttgccc gagatcgacg cagcggcgtt cacgacggcg
     6001 gcggggcgac aggcgacgag ggcctcggcg ctcacccggt cgaggtcctc gtggtcgatc
     6061 acggcgatgt caccgggccg aagccgcttg gtcaggttct tcgtgcgacg gtccactcga
     6121 gcagggccgg tcagccccgg gaggggacca cggccacggc gggagggtag gcgaatcgac
     6181 atcggcacca tcgtgccacg tgacgctagc cgcgagcgcg agccatcagg tctcgggcgt
     6241 gctccagggc ggcgtcggtg cccgagccac ccagcatgcg cgcgagttcg gccacgcggt
     6301 cctcccccgt gacctcgtgc acgccgctgc gggtcacgtg gccgtcgtcc gagcgctcga
     6361 cgacgaggtg tcgatcggcg tgggccgcca cctgcgccag gtgcgtcacg acgatcacct
     6421 gggcgtggcg ggccagagcg gccagccggg ccccgacatc gagggcggca cgcccaccga
     6481 ccccggcgtc gacctcgtcg aagacgaacg tcgggacgct ggcggtctcg gtcgccgtgg
     6541 cgacctcgag ggcgagcatc acccgggaga gctcaccacc ggatgccgcc ttggtcaccg
     6601 atcgcggtgc cgtgcccgac ccggacgcga gccggatctc cacgtcgtcg cgcccctcgg
     6661 cgccgaagcg accggcatcc gcgacgtcca cggtcacggt ggcggacccc atggccaggt
     6721 gcgtgagctc ctcggtgatg cggtccccga gggtggatgc agcggcggtg cggcgcgccg
     6781 tgagggtggc ggcagcccgc tcacgctcgg cggtgagcac ctcgacgcgc gcggcgagct
     6841 cgtcgatgcg atcggtggtc gactccagct cggctacccg cacggctgcg tcacgcgccc
     6901 aggcgagtac gtcgtcgacg cctggcccgt agaggcgggt cagttcggac agtgcggctc
     6961 tgcgcgactc gacctgctcg agccgggccg ggtcgatgtc ggcgtcggcg aggtaggagc
     7021 cgaggtcggc cgcgacgtca gcggcgaggt agcgcagctc gtcgacccgg gtgccgatct
     7081 cggccagggc cggatcgctg gcgctgacct ggtcgaggcg cgccgatgcc gtcgcgagcg
     7141 cccccaggac gcccgggttc gcgggaccgc cggcgtcgtc gccgccggtg agcgcgtcgt
     7201 gcgccgcacc ggcaccgtcg cgcagctcct cggcgtggcc gagccgctgc gactccacgc
     7261 gcagggcgtc gtcctcgccc ggttggggat ccagggcctc gatgcgctcg agcccgaccc
     7321 gcagggtctc ggcctcgcgc gcacgggcgg cggccgcggc gcgcaggtcg cccagctcgg
     7381 cctcggcccc gatcagctcg ccgtggaccg cccggtagtc ggtgagggcc gtcgcgacgt
     7441 cgcccccgcc gaagccgtcg aggacctcac ggtgctggtc cgcgcggcgc agccgccact
     7501 ggtcggcctg cccgtggacg gcgacgaggt gctcggcgag ctcggccagc agtccgacgg
     7561 gcgcggcccg accaccgacg tgtgcccggg acctgccgtc cgacgcgacc gagcgcacga
     7621 ggacgaggcc gtcgcgaacg tccgccccgg catccgcggc ccggcccagc gcccggtggc
     7681 cctcgggcag ggacagcacc ccttcgacga cgacgcggtc ggcaccggcg cggaccaggg
     7741 cagagtctcc gcggccgccg aggagcaggc ccagcccggt gacgaccatg gtcttccccg
     7801 cgcccgtctc ccccgtcagc acggtcagcc cggggtgcag cgggagcact gcttcgtcga
     7861 tgacaccgag gtcgcggatg cggatctcct cgatcacggt gagccgtccc cctgggccag
     7921 ctggcggcgg gcggcaccgc gccacccctc gacggggagg tcgaacttct cgacgagtcg
     7981 gtcggtgaag cccccggagt tcaggcgggc caggcgaacc ggttcatcgg aacggcgcac
     8041 gtggatccgc gcgcccgggg ggaggccgac cgcccggcgc ccgtcgcacc acaccacccc
     8101 gtgggcgtcg gtctgcgcga gcacctcgac gacgaagcgc gactgcgggc cgacgaccac
     8161 ggggcgcgcg aacagggcgt gcgcactgat cggcacgagc agcagggctt cgacatcggg
     8221 ccagaccacg gggccacccg ccgagaacgc gtacgccgtg gacccggtcg gggtggccat
     8281 gacgaccccg tcgcagcccc acgtcgacag cgggcgcccg tcgatctcga cggtcagctc
     8341 gagcatccgc tcccgagacg ccttctcgac cgtgacctcg ttgagcgccc acgactggaa
     8401 gaccgtcctg ccgtcctcct ccgcgcgcac ctcgagggtc gtgcgctcct ccacgtggta
     8461 ctcacgagcc acgatgcgtt ccacggtcgc gtcgaggtcc tcacgctcgg cttcggcgag
     8521 gaagcccacg tgcccgaggt tgaccccgag aagcgggacg cctgacccgc gggacagctc
     8581 ggccccccgc aggatcgtgc cgtccccacc gaggacgcag accagttcac agccggctgc
     8641 tgccgattcg tcggtgtcgg tgtcgacgcc gagcacgccc ggcacccgcg ccagtggggt
     8701 gtcgacgagc tcgtccctgg acgcgctcac ccccacgccg gcggcgatga gccgggtggc
     8761 caccccggct gcgagttcct gggcgtcagt ccgccagagg tgcgtgacga gcaggatgcg
     8821 acgtgacggg gtgctcgtca tgctcatcct ccgcgcgcct cggtggtgat ctcgcgaacg
     8881 atgtcgcgga cccggtcagc gctcattgtg tccgacgggt ccgaccgggc ccacaggagg
     8941 tactcgatgt tgccggtggt gcccgcgatc gggctctcgc ggatgccgtg cacgaacagc
     9001 cccgcgtcga gggtcgactc gacgacccgc tggagggccg ccgtccgctc gcgcgggtct
     9061 gtcaccacgc cctgccggtt cagccggccc cgcccgacct cgaactgtgg cttcacgagc
     9121 acgacgaggt cacctcgctc gcccatcagt cgagcgaggt cggcgagcac gagggtcaac
     9181 gagatgaagg acaggtcggc cacgacgaca tccgcggggc cgccgatggt ctccggctga
     9241 agtccgcgga tcgtcgtccc gctgtgctcg gtgacgcgtg ggtcgctcgc cacgtcgggc
     9301 acgagctgcc ctcgtccgac gtcgacggca gcgacatgcg ctgcgtcggc ggcgagcagc
     9361 acctgggtga agcccccggt ggaggcaccc acgtcgacac accgacgccc cttcacggtg
     9421 aggccgacgg gtccccattc ctgcagcgcc gcgtcgagct tgtgggcagc cctgcccacc
     9481 caacgggggc cggcgacctc gaccacgagg acgtcgtcgt cggccacggg cagcgacggc
     9541 ttggtcgcca ccgagccacc gatcgtgacc tgtccggccc tgacgaactc ctgcgcctcc
     9601 ccccgagagc gtgccagccc ccgccgtacg agctcggcgt ccagacgtgc gatgtcagcc
     9661 gccgaggtcg gcgagccgcc cctgcagggt gcggtggacg gcctcaccgg cctggagctg
     9721 accgtcgagg tcacccgccg gggtgttcgc gagatcgcgc agtgcagcgt cgatgacgat
     9781 gtcgccggtt tccggcggcc cggcctgctg gtcctgctgt gctgcgtcgt cgatgtccgg
     9841 caacctcacg cgtcactcgc gggagtgctg ccggatgccg gtgccgacgg agcctgcgtc
     9901 gcgggcgagt cacttgatgc cgtgctcttc gcagccggtg cagacttcgt ggcggccttc
     9961 ttcgccggcg ccttcgcggc cgccttggcg gtagccgacg tcgtggcagc cttcttcgcg
    10021 ggtgccttgg cagccggagc cttcgcggcg gccttcgccg gacccgacgt cgttgcggtc
    10081 gccctcctcg ccggcgcctt cgcggcggcc ttggccgccg gagccttagc ggccgccttc
    10141 gccgtggtgg cgctcgcctt cttcgccgga gccgccttcg tggcggcctt cttcgccggn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccggagcctt ggcggccgcc
    10321 ttggcgggag ccgccttcgt ggtggccttc ttcgctggcg ccttctttgc cacccgtgcg
    10381 ggagccttct tcgccgcggc cttcaccggc tcaggggcga cctcgacgat gtcggagacc
    10441 acggttgccg caccgcggat ctcgacggag gcggcgcttc gggccagctc ctccacctgg
    10501 gaggccaggg tagccaccgt tgcgcccagt ccctggacct cacgggtgag gcggtcgacg
    10561 tcgagcttgc cgacggcgga gtcgacctcg cgccgcacga ggcgcacgat ctgcttgcgg
    10621 ttggcctcgg ccgccttgag caggtcgtct gccagcgcac tggcctgctt ggcgagcttc
    10681 ttgcgcgagg tgtcctggct ggcgagagcg atgatctcct gagccgcctc cttcgcccgg
    10741 cttcgggtga ggtcacccag cccggaggcg agttcgacgt agccctggat ggagttcttg
    10801 gccatggatc ctcctcgatc tggcagcccc gtcggccacc cgctgccgcc cacgctaccg
    10861 aaagcggacc gctgcgagca cggactcggc cgactcactc agtgcggcga tgtccgaggc
    10921 agggcttcca gcgtcccgtg cccggtggac ggcgaccact gctcgtgaca atgcgtggag
    10981 ccagtcaccc tctgtgtccg ggtccacgtt cagggcgcgc aggtcgggcg cgacccaggt
    11041 ggggcgccgg tggggaggcg cctcgaggca ggcagcgaga tcgtcgacac cggtcaggac
    11101 gagcagggag tccatgcccg cggcgacggc cccctcgatg tcggtgtcga gccggtcacc
    11161 gaccgccagg acacgctctg ccggaatcgc cagccggtcg gcgcacatga gatacagcgg
    11221 cgctgacggc ttgccggcga ccatgtccgg agcgtgaccg accgcacgct gcaccgcgcc
    11281 gatgagcgag ccgttccccg gtgcgacacc acggttggtc gggagtgtcg catcggtgtt
    11341 cgtcgccacc caggtcgcgc cggcctgcac ggcgtaggca acctccgcga ggtctgacgc
    11401 agtgacccct gcgccgtacc cctgaagcgc ggccacgacg tcgtgcccga gtccgtcggc
    11461 cggaacgacc gggaccagtc cgacctggac cagtgcggca gacactcccg ggccgcccac
    11521 ggcgaggact ctgctggatg ggggcacgtt gtccgccacc agccgtgcgc cggcctgcga
    11581 gctggttgcg acgtcaccgg gctcacagtc caggcccagg tcgcgcaggt gagcggcgac
    11641 gtcctccgga gtgcgcgacg cgttgttcgt ggcgtagatg acggggaggt tgctgctcgt
    11701 cagggcctcg atcgcatgcg ggaccgccgt cgggccccgg tagacgaccc cgtcgaggtc
    11761 gcagacgatg gcggcgtagc gcgcagccag ggtctgcggc ccgctcacac gtcctccgga
    11821 gcgacagccg tcacctcgga gagctcagca cagtcgtcct cgtcgtcctc accgaggtcg
    11881 acgacgatcc cgtcgagctc ctcgagtcgc tccacggcat ccgtcaccgc gtcgacgtct
    11941 ccctcgacgg ccttgaggaa ccaggcgcgc gcctcctcgt cgcggccgag tccgagaagg
    12001 acgtcggcgt aggcgtagtg gagccggcac acggacggac ccgcagcacg gcgcagcgca
    12061 gggatctgga gcgccaccag tgccgcgtca tcctggccga ggtctcgccg gatacccgac
    12121 accacgatcg ccaactcctg ccgctcgtcc tcggtgagcg tgcgggcttc gggcgtggct
    12181 gccagctcaa gggcacgcga gtgccggccc agcccgcgct cgcagtccac catcagcggc
    12241 agcaggtggc tggaaccact gagacggcgc accgtccgga actcggtcag ggcgcgcgcg
    12301 aagtcaccga gcctgtaggc caccatgccg agcgcctcgc gggctgccgg cacgcgccca
    12361 gcacgccgca cggccgtctc ggcatgggcc agggccgctt cgaggttgtc agcctcgagc
    12421 gttgcagcaa ccatgacgag gtgctgggcc accccctccg cgttctcctt ggacagggtg
    12481 cgcaactgct ggtgcacagc acggtcgagt tccttgcccg tcacctcgtc cggaatgcgg
    12541 ggctcgggca gccgggggcc cttgggtgcg agtcggctcc caccacggtc atcgcgctcg
    12601 cccgctcgag cgggcttccc ctcacgaccg gtgtcgcgcc cagcacggcc ttgcggacgc
    12661 gccccggaac cactgcgatc gtcacggcga cggtcgggac cagcgccccg atggccgccg
    12721 gtgcgacggt cttcaccact gcgcgaccca cctcgacggt cagccccgcc gcggctgggc
    12781 gcaccaccct ggcggggcgc cctgtcgtag gacgatgacc gagggggtcg gctctggtca
    12841 cgccgaggtt ccgacacacc gtcccgcgag ggctcgccct ccgggcgctc gatgttgctg
    12901 tcctcggtca cgccatcatc tcctgttgtt cgtggccgcg cgttgactgc tgtcaccggc
    12961 cgacctcgtg tgccacgcac tccgcacatg acgcccgctc actgtgctga caccgcgtcg
    13021 cgcgccgacc aagtctacgg accttcctcg cccctgcgga cacagccact gcctggcgtc
    13081 gcacgagctc atcgacaatg tgccggggaa tgcggaagag ggcccaccaa tcatggtggg
    13141 ccctcttcgg taatggtt
//
LOCUS       DAOSOC010000051        13106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4764_length_13106_cov_10.153245, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000051 DAOSOC010000000
VERSION     DAOSOC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 13106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13106
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4764_length_13106_cov_10.153245"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(445..939)
                     /locus_tag="PL014_05775"
     CDS             complement(445..939)
                     /locus_tag="PL014_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOT56635.1"
                     /translation="MADSSFDIVSKFDKQEVANAVNTAAKEISNRYDFKGVGATVELA
                     GEVIKIEANSDERCKAVLDVVETWLIKRGVSLKHLDIPEAGPRLSGKEYKLDVPLKEG
                     ITSENAKKITKIIRDEGPKSVKTQIQGDEVRVTSKSRDDLQEVQRLLKAADLDIALNF
                     TNRR"
     gene            1003..1083
                     /locus_tag="PL014_05780"
     tRNA            1003..1083
                     /locus_tag="PL014_05780"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1037..1039,aa:Tyr,seq:gta)
     gene            1240..2472
                     /locus_tag="PL014_05785"
     CDS             1240..2472
                     /locus_tag="PL014_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT56636.1"
                     /translation="MHTEAATAAPAPSLARRVWLVVGVLALTAALVFMLWRGASTFYY
                     VIMAWFAALAMEPVVSRLAKRMHRGLATGLVMLLAGISLVVFLGLFGTLFVEQLTSFI
                     TALPGMAEDTLRWFNRVSGSTFTFETILDEIGLSPTDLTAYADDLAFGVLGLVAKVLS
                     GFFGVFILVFFTFYMSAGMPTLRGWVARRLRPSMQVPFLTAWDLTGIKVGGYIAARIV
                     LASINAVLSGVVFALIGLPYWLPLALWTGLVAQFVPNIGTYIAIALPVLVGLTSGDPM
                     VGVWVLAWGIGYQQVENLAIEPRISSRAVNLHPAVSFGSALLGAQLFGLSGALLGVPV
                     AATVMAMLEIYQRRYELSPQTEELVAELVAPRATQRDAGGEGSENGDVDAVPPPLAGL
                     APPVAAEVSHPPDEIRTD"
     gene            2485..3447
                     /locus_tag="PL014_05790"
     CDS             2485..3447
                     /locus_tag="PL014_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56637.1"
                     /translation="MGSRSRRILAITLLVLAGLLVPVAVVATWTARTVTDTESFVSRV
                     GPVASQPEVQALVSTQLADQVTGAVIDDRVAPRVGEAIDELAAPELVKGLLRDVAASL
                     GGAVEARVQNVADKIVTSPEFQSAFDTALDEAHAELVATLEGDEPQGTVITEGTTVSI
                     SLATIAGAVRAELVNSGYTFVDRLPQLEATVPIATVEQLEKWQGYYSLLKVMVWLGPL
                     LVVVLLAAGAWLLRDAAVSALWFAGSGFLALVAVTVGVRAAVGSATSDIADPLAADAA
                     RVIVATLTTTLVRNATVVGLLLVIALAAAAYVAARRRLTPTTAS"
     gene            complement(3473..4447)
                     /locus_tag="PL014_05795"
     CDS             complement(3473..4447)
                     /locus_tag="PL014_05795"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOT56638.1"
                     /translation="MKTPNPNPQPSTDRPSDTGPTAETAAPSTAAARTAARLAPLVAW
                     ADRNPVPGTTLRIVRQLLAVDVRDRVFGMAGQSFLAVIPLLIISSSWLSQSDGRALAT
                     ALNTRLGLTGVTADTVTLLFSRPEGSAEVSTASGLSLALLFFSVNSFTRTLRRSIEGP
                     WELPKVGWKGQLSGLLGVVLLIVMQVTLAVIAVEWVSTSAAQGFLEGLVRTVVATAFW
                     IAIGRALTHGRLSVARLWPGALVGGVGSTAIAIWTVTVLPGIFERDAARYGVIGVALA
                     LVTWLLAFSGLIVLVGVAGAQIARAAGWFTPREDPLVQPLFEQPDPQA"
     gene            complement(4444..4815)
                     /locus_tag="PL014_05800"
     CDS             complement(4444..4815)
                     /locus_tag="PL014_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56639.1"
                     /translation="MRIAAVAAALVALFAPVVRLVEQVSKCGPGEPFTRPTTYARVAT
                     APGREGPALQYWLYHPFTAVNNKHESDWEMIRLEFAASDAASALEQDPTCLGYGHHEG
                     VEVAEWSSVKLERLPAEVTLS"
     gene            5067..10601
                     /locus_tag="PL014_05805"
     CDS             5067..10601
                     /locus_tag="PL014_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT56640.1"
                     /translation="MFQRIAIVNRGEAAMRLIHAVRDLNAQSGPGGHRIETVALHTTA
                     ERSAMFVREADAAYDLGPASERPYLNHEVLERALRETGADAAWVGWGFVAEDPAFAEV
                     CARAGVTFIGPSPEAMRRLGDKIGSKLIAEEVGVPVAPWSRGGVDTLEDAIAAAGRIG
                     YPLMLKATAGGGGRGIRMVATDADLADAYERTRDEAQRAFGSGVVFLEKLVTGARHVE
                     VQVIADGQGTAWAIGVRDCSVQRRNQKVIEESASPVLDAEQMRELKDAAERLAIAVDY
                     AGAGTVEFLYHPGERFFAFLEVNTRLQVEHPITEVVTSTDLVRLQIHVASGGRLEGER
                     PSEVGHAVEARLNAEDPDRDFAPAPGRIAHLELPAGPGIRVDTGVAEGDSIPADFDSM
                     IAKIIAHGRDRDEALARLRRAMRETTVVIEGGATNKSFILDLLDQPEVIDGSADTGWI
                     DRVRSQGRLVASEHSGVALVVAGIEAYEEAESVERTRLLHTSRGGRPQVQHDVGRAVD
                     LKLRGTAYKVTVFRTGPARFRVAVADPTGHTVTVDADFERGGPYASRIVVGGRSHRLV
                     TAAHGAVQLVEVDGVTHRVSRDEGGVMRSPAPALVVATPVAVGDEVAAGAPVLVLESM
                     KMETVLPAPFAGRVKELLVAAGSQVETGAALVRLEPTGDPADAGAAADVDAPDLDLPD
                     EVTDREPTERAARGRADLAAMLLGYDLDPSQEQRTLTGYLEARDEIEASGASPINDEV
                     VLLGVFADFAELSRNRPVGEEAHLENRVHSPREHFHTYLQTLDPERGALPADFVGRLE
                     RVLSHYGVTSASRDDAGDAAAADALEGAVFRVFLAQQRSAPEVAMATAILQRWLGEPA
                     PEPPADVAARELLDRLVSATQLRFPIVGDLARSVRFRWFDQPLVDADRASVLGSVGDE
                     LAEIDALPDGPERTARIDTLAAIPERIVRFLAERLRTGIQAREPMLAVLVKRHYREYD
                     LVDLREETIDGRGVASANYVLDNRVSHLITTTGTVDELTDGSPLSTLLSGLVAQRPVG
                     HQGVVDLYVHWGDLPADKEAASAALAEKIARMPFAAQVRRVAVGVVPDDEREVGYFTF
                     RPQGDGSLVEDRPVRDVHPMVGRRLNLWRLRDFDLTRIDAPEDVLLLHAVATGNPQDQ
                     RLVALAQVRQLVVVRDAAGKVTGLPHIERALANCLEAIRRARATLGAGARLDTNHVWL
                     HVWPPVEADLDQLTALQGKVAPMTAGAGIEEVRVEGAIPGEDGTTVPICARFAYQPGS
                     GVTFTVDQPPTTRVAPLDEYAEKVVRARRRGLVYPYELVSMVAGSGGSVQELDLDESG
                     RLVPVERPYGENTAGLICGLVTTPTPLHPEGVSRVLLCGDPLRALGAVAEPECARVIA
                     ALDLAEEWGLPVEWFALSAGARISMDSGTENMDWVAKALKRIIEFTQAGHEINVVVAG
                     INVGAQPYWNAEATMLMHTKGILVMTPDSAMVLTGKQSLDFSGGVSAEDNFGIGGYDR
                     VMGPNGQAQYWAKDLVDAYGILLAHYENTWVAPGESGPRRAPTSDPHDRDVTTHPHTL
                     AGSDFTTVGDIFSSATNPDRKKPFDIRTLMRAVADQDHGTLERWAGMADAETAVVLDA
                     RIGGIPVCLLGIESKAVARRGVPPTDGPDVYTAGTLFPRSSKKAARAINAASGNRPLV
                     VLANLSGFDGSPDSMRALQLEYGAEIGRSVVNFDGPIVFVVVSRYHGGAFVVFSKALN
                     PRMTVLAVEGSFASVIGGAPAAAVVFARDVDKRTAADPRVADLEARVAAASGAERAAL
                     ATELIAVRTAVRAEKLGQVASEFDRVHDIHRAVQVGSVDAVISASEIRPRIIAAIEAT
                     TA"
     gene            10701..11327
                     /locus_tag="PL014_05810"
     CDS             10701..11327
                     /locus_tag="PL014_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT56641.1"
                     /translation="MTTVGLLLAAGAGRRMGGPKALLRLTPTGPTLIETAVERLWQAG
                     CDRVVVVLGAGASQASDAVAHLRVDVVTVPDWAEGMGASLRAGLADLSSVAPQEVAAD
                     IALVTLVDLPDVTAEVMARVLAAAQPQGAAALVRAAYGGVPGHPVAIGRDHWEAVRTT
                     ARGDRGARDHLRTTEHDLVECGDLASGRDVDTVADLPGPHRWRDMMGP"
     gene            11324..11695
                     /locus_tag="PL014_05815"
     CDS             11324..11695
                     /locus_tag="PL014_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HOT56642.1"
                     /translation="MSAPEHRWPGSVYDHGSDPDPRFSLANERTFLAWIRTALALVAG
                     AAAVDALPLPMPDAVQRALAAGLALAALLTAFAAWRGWAGTERAMRQGKSLPSNPAMV
                     VVLVAVAVTAVVLGITSVTGA"
     gene            11749..12084
                     /locus_tag="PL014_05820"
     CDS             11749..12084
                     /locus_tag="PL014_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HOT56643.1"
                     /translation="MSRPVGDPAAPQERTTLAWRRTGLALLIGALTIGRLTLDALGPA
                     VVVPTALAAALAGWVIIAALRTRRLARAHPGEPGFSVLADGRLPAVVAALVGALAVGE
                     LLAAVAHLL"
     gene            12178..13068
                     /locus_tag="PL014_05825"
     CDS             12178..13068
                     /locus_tag="PL014_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT56644.1"
                     /translation="MDPRTTFDSAADLEGALGATGYLTDEALATVTWLALRLGRPLLM
                     EGEPGTGKTALAEALAQAMDLPLIRLQCYEGIDATQALYDWDFPRQILHVRALEAVED
                     GPRDVRDVEEGLFDERFLLARPVLRALRESPAVLLIDEIDRADDEFEAFLLEVLSTWQ
                     VTIPELGTVTASVPPVVILTSNRTRELHDALKRRCLYHWLEHPGIARELAIVRSRAPE
                     VPEALADQVVRLVHGIRTDRELLKPPGVAETLDWARALNELGTRELDTATAAATLGVA
                     VKYREDAERVRRALDIMLTT"
BASE COUNT         1780 a         4840 c         4539 g         1947 t
ORIGIN      
        1 ggtcagtgcc atgcacccga cgtcgctggt cgaggacggc acgctcgagg cgtcggcggc
       61 catgctggcc cgggctgacc tgacgcctgg tgtgcggcgc gcgctggtcg acgcgaacca
      121 cgagctgcgc gaggcactgg ccagtcgccg acgcttcgac tgagccccgg ctggctgccc
      181 gggtggcgcc cggctggtct ggctgcctcc ggcggctggg gggcgcgcgg cacttctccg
      241 tcatcccggt gatggcggag aagttccctc atggcgcgga tatatgcccg ccaagcctgg
      301 agttccccgt cgtcccagtg atgacgggga acttcaggtc gtcaggagct ctgctcggac
      361 ccggcccaac gagaccggat gccgtccgcc cgaggtgggc ggacggcatc cggtctcgcg
      421 tcggcccccg atgggggcgc cggctcagcg gcggttggtg aagttcagcg cgatgtcgag
      481 gtcggcggcc ttgagcaggc gctgcacctc ctggagatca tcgcgcgact tgctcgtcac
      541 ccgcacctcg tcaccctgga tctgggtctt cacgctcttg ggcccctcgt cgcggatgat
      601 cttcgtgatc ttcttcgcgt tctcggaggt gatgccctcc ttcagcggca cgtcgagctt
      661 gtactccttg cccgacaggc gggggccggc ctcggggatg tcgaggtgct tgagcgagac
      721 gccacgcttg atcagccagg tctcgacgac gtcgaggacg gccttgcagc gctcgtcgct
      781 gttcgcctcg atcttgatga cctcgccggc cagctcgacg gtggccccca cgcccttgaa
      841 gtcgtagcgg ttcgagatct ccttggccgc ggtgttcacg gcgttggcga cctcctgctt
      901 gtcgaacttg ctgacgatgt cgaacgagct gtccgccacg ggtcctcctg gagtcgattc
      961 ggtgtgccgg gttgcggctt gctatccttc catgcgcacc acggcaggtt gcccgagcgg
     1021 ccaatgggag cggactgtaa atccgtcgcg aaagcttcga aggttcgaat ccttcacctg
     1081 ccacccgtgc tgaaaaggcc cggacgaccc tgtcgtccgg gccttttcgc acccggcgca
     1141 ccgcgcccga accgacttct tgcgctccag ccacctggtt gtccacaggc atccgcggcc
     1201 ggcacgcgac cagcgggtcc acgacataga ttgagcgcca tgcacaccga ggccgccacg
     1261 gccgcccccg caccatccct cgcgcgccgg gtctggctcg tcgtcggggt cctggcgctg
     1321 accgccgccc tggtcttcat gctctggcgg ggcgcctcga cgttctacta cgtgatcatg
     1381 gcgtggttcg cggcgctggc catggagccg gtggtctcgc ggctcgcgaa gaggatgcac
     1441 cgcggtctgg ccaccggcct cgtcatgctg ctggccggca tctccctcgt ggtcttcctc
     1501 ggcctgttcg gcaccctgtt cgtcgagcag ctcacgagct tcatcaccgc actcccgggg
     1561 atggccgagg acaccctgcg ctggttcaac agggtgtccg ggtcgacgtt cacgttcgag
     1621 acgatcctcg acgagatcgg cctgagcccc accgacctca cggcgtacgc cgacgatctg
     1681 gcgttcgggg tgctcggcct cgtcgcgaag gtgctctccg gcttcttcgg cgtcttcatc
     1741 ctcgtgttct tcacgttcta catgtccgcg gggatgccca cactgcgggg ctgggtcgct
     1801 cgccgcctgc gcccgagcat gcaggtcccg ttcctcacgg cctgggacct caccggcatc
     1861 aaggtcggtg gctacatcgc cgcgcgcatc gtgctcgcgt cgatcaatgc cgtgctcagc
     1921 ggcgtcgtgt tcgccctcat cggcctgccg tactggttgc ccctcgccct gtggaccggc
     1981 ctggtcgccc agttcgtacc caacatcggc acctacatcg ccatcgcgct cccggtgctc
     2041 gtcgggctga cgtcgggcga cccgatggtg ggggtctggg tgctcgcgtg gggcatcggg
     2101 taccagcagg tcgagaacct cgccatcgag ccgcgcatct cctcgcgcgc ggtcaacctc
     2161 catccggccg tgtcgttcgg ctcggccctg ctcggtgccc agctgttcgg gctgtcgggt
     2221 gcgctgctcg gggtgcccgt cgccgccacc gtcatggcga tgctcgagat ctaccagcgc
     2281 cgctacgagc tgagccctca gaccgaggag ctcgtcgccg aactcgtcgc gccacgcgcc
     2341 acgcagcgcg atgccggggg agaaggcagt gagaacggcg acgtggatgc cgtccccccg
     2401 cctctcgcgg gcctggcacc ccccgtcgcg gccgaggttt cccatccgcc ggacgaaatc
     2461 cgcaccgact gagaagatcg cgccatgggc tctcgttccc gccgcatcct cgccatcacc
     2521 ctgctcgtcc tcgctgggtt gctcgtgccc gtggccgtgg tcgccacctg gacggcccgc
     2581 acggtgaccg acaccgagtc gttcgtgtcc cgggtcgggc cggtggcctc gcagcccgag
     2641 gtgcaggcgt tggtctccac gcagctcgcc gaccaggtga cgggtgccgt gatcgatgac
     2701 cgggtggcac cgagggtggg ggaggccatc gacgagctgg cggcgccaga actcgtcaag
     2761 ggcctgctgc gtgacgtcgc ggcctcgctc ggcggtgccg tcgaggcacg ggtgcagaac
     2821 gtcgccgaca agatcgtcac gtcgccggag ttccagtcgg cgttcgacac cgcgctcgac
     2881 gaggcccatg ccgagctggt ggcgaccctc gagggggatg agccgcaggg caccgtcatc
     2941 accgagggca ccacggtgag catcagtctg gccacgatcg ctggagccgt gcgggccgag
     3001 ctcgtgaact ccgggtacac cttcgtcgac cggcttccgc agctggaggc caccgtgccg
     3061 atcgccaccg tcgagcagct cgagaagtgg cagggctact acagcctgct caaggtcatg
     3121 gtgtggctcg ggccgctgct cgtcgtcgtg ctgctcgccg ccggagcgtg gctgttgcgc
     3181 gacgccgccg tgtcagccct gtggtttgcc gggtccggct tcctggccct ggtcgccgtc
     3241 acggtgggtg tgcgcgcagc cgtgggctca gcgacctccg acatcgcaga cccgttggcg
     3301 gccgatgcgg cccgggtcat cgtggccacc ctgacgacca ccctcgtccg caacgcgacc
     3361 gtcgtcgggc tcctgctcgt catcgcactc gccgctgcgg cctacgtcgc ggcccggcgc
     3421 cgactcaccc ccaccaccgc ttcctgagca gtcaggtcag cggctgggag gatcaggcct
     3481 gcgggtcggg ttgctcgaac agtggctgca cgagcgggtc ctcacgcggc gtgaaccacc
     3541 cggcggcccg ggcgatctgg gcgccggcca cgcccaccag gacgatgagg ccactgaagg
     3601 ccagcagcca ggtgacgagt gcgagggcca cgccgatgac gccgtagcgc gcggcatccc
     3661 gttcgaagat gccgggcagc acggtgaccg tccagatggc gatggcggtg ctgccgacac
     3721 cgccgacgag cgcgcccggc cacagccgtg cgaccgacag gcgcccgtgg gtcagggccc
     3781 gcccgatcgc gatccagaac gcggtcgcca ccaccgtgcg gaccagccct tcgaggaacc
     3841 cttgagcggc cgacgtgctg acccactcca ccgcgatcac cgccagggtc acctgcatca
     3901 cgatgagcag caccacgccg agcaggccgc tcagctggcc cttccacccg accttcggca
     3961 gctcccaggg cccttctatg ctgcggcgca gggtgcgggt gaagctgttc accgagaaga
     4021 acaacagcgc caggctgaga ccggatgccg tgctgacctc cgcgctgccc tccggtctcg
     4081 agaacaacag cgtcacggtg tcggcggtga cgcccgtgag ccccagccgg gtgttgaggg
     4141 ccgtggcgag tgcccgcccg tcgctctgcg agagccagct cgagctgatg atgagcagcg
     4201 ggatgacggc caggaacgac tgccccgcca tgccgaagac gcggtcgcgc acgtccaccg
     4261 cgagcagctg gcgcacgatg cgcaaggtgg tgcccgggac ggggttgcgg tcggcccagg
     4321 cgacgagcgg ggccagccgg gcagcggtcc gggcggcagc ggtgctgggc gcggcggtct
     4381 cggcggtggg gccggtgtcg gagggtcggt cggtggacgg ctgcgggttc gggttcgggg
     4441 tcttcacgac agggtgacct cagcaggtag gcgctcgagc ttgaccgagc tccactcggc
     4501 gacttccaca ccctcgtgat ggccgtaccc gaggcaggtc gggtcctgct cgagcgcgct
     4561 cgcggcgtct gacgccgcga actcgagccg gatcatctcc cagtcgctct cgtgcttgtt
     4621 gttgacggcg gtgaacgggt ggtacagcca gtactgcagc gccggaccct cgcggcccgg
     4681 cgcggtcgcg acccgggcgt acgtcgtcgg cctggtgaac ggctcaccgg gcccgcactt
     4741 actcacctgt tccacgaggc gcaccacggg ggcgaaaagt gccacgagcg cggcggcaac
     4801 ggcagcgatg cgcatccggc cacgatacgg ctcaaatttc cagcccgatg ccacatcatt
     4861 caccttggaa cccttccttt ctggtgctga gatgaacctc ccggtgtgaa ggtcgcgcac
     4921 cgacgactgg cgagctcgat cgcagccatg gcgcgcgcag tctgttcatt actcgcgcgt
     4981 cacggcggac aagtgacagg ctcggacgac cagcgggatc gccccgccga gacgacgcgc
     5041 acaccggacc cccgggagga ccaccgatgt ttcagcggat cgccatcgtc aatcgcggag
     5101 aggccgcgat gcggctcatc cacgcggtac gcgacctcaa cgcgcagtcc ggcccgggcg
     5161 gccaccgcat cgagaccgtg gccctgcaca ccaccgccga gcggtcggcg atgttcgtgc
     5221 gcgaggccga cgcggcctac gacctcggcc cggcatccga gcgtccctac ctcaaccacg
     5281 aggtgctcga gcgggcgctg cgtgagaccg gtgccgacgc ggcctgggtg ggctggggct
     5341 tcgtcgccga ggaccctgcc ttcgccgagg tctgcgcccg tgccggggtg acgttcatcg
     5401 gcccgagccc cgaggcgatg cgacgcctcg gcgacaagat cggctccaag ctgatcgcgg
     5461 aggaggtcgg ggtcccggtc gctccgtgga gtcgcggcgg ggtcgacacc ctcgaggacg
     5521 ccatcgccgc ggccggccgg atcggctacc cgctcatgct caaggcgacc gcgggcggtg
     5581 ggggccgcgg catccgcatg gtggcgaccg acgccgatct cgccgacgcc tacgagcgca
     5641 cccgcgacga ggcgcagcgg gccttcggct cgggagtggt gttcctggag aagctcgtca
     5701 ccggcgcacg ccacgtcgag gtccaggtca tcgccgacgg ccagggcacg gcgtgggcga
     5761 tcggggtgcg cgactgctcg gtgcagcgcc gcaaccagaa ggtgatcgag gagtccgcct
     5821 cgcccgtgct cgatgccgag cagatgcgcg aactcaagga tgccgcggag cggctcgcga
     5881 tcgccgtgga ctacgcgggg gctggcacgg tggagttcct ctaccacccg ggtgagcgct
     5941 tcttcgcgtt cctcgaggtc aacacccgcc tgcaggtcga gcacccgatc accgaggtcg
     6001 tgacgagcac cgacctcgtg cggctccaga tccacgtggc gtcgggcggc cgcctcgagg
     6061 gtgagcgccc gagcgaggtc gggcacgccg tcgaggcccg gctcaacgcc gaggaccccg
     6121 accgcgactt cgccccggca ccggggcgca tcgcccacct cgaactgccc gcaggccccg
     6181 gcatccgggt cgacaccggt gtggccgagg gcgattcgat ccccgcagac ttcgactcga
     6241 tgattgcgaa gatcatcgcc cacggtcgtg accgggacga ggcgctggcc cggctgcgtc
     6301 gtgccatgcg cgagacgacc gtcgtcatcg agggtggggc gacgaacaag tcgttcatcc
     6361 tcgacctgct cgaccagccc gaggtcatcg acggctcggc cgacaccggg tggatcgacc
     6421 gcgtccgctc gcagggtcgg ctggtcgcga gcgagcactc gggtgtggcg ctcgtggtcg
     6481 ccggcatcga ggcctacgag gaggccgagt cggtcgagcg cacccgactg ctgcacacct
     6541 cgcgcggcgg gcgcccccag gtgcagcacg acgtcgggcg cgccgtcgac ctcaagctgc
     6601 gcggcaccgc ctacaaggtc accgtcttcc gcaccggccc tgcccggttc cgggtcgcgg
     6661 tggccgaccc caccggacac accgtcaccg tcgacgccga cttcgagcgc ggcggcccct
     6721 acgcgagccg catcgtcgtg ggcggccgtt cccaccgact cgtcaccgcc gcccacggtg
     6781 cggtgcagct cgtcgaggtc gacggggtca cccaccgggt cagccgtgac gagggtggcg
     6841 tgatgcgttc ccccgcaccg gctctggtcg tcgccacccc ggtcgccgtc ggcgacgagg
     6901 tggccgcggg tgccccggtg ctcgtcctcg agtcgatgaa gatggagacc gtgctgccgg
     6961 cgccgttcgc gggccgggtc aaggagctgc tcgtcgccgc gggcagccag gtcgagaccg
     7021 gcgccgccct ggtgcggctc gagccgacgg gtgacccggc agacgccggg gctgccgccg
     7081 acgtcgacgc ccccgacctc gacctgcccg acgaggtcac cgaccgcgag cccaccgagc
     7141 gggctgcccg gggccgcgcc gacctcgcgg ccatgctcct gggctacgac ctcgacccca
     7201 gccaggagca gcgcacgctc accggctacc tcgaggcccg cgacgagatc gaggcgtcgg
     7261 gcgcatcgcc gatcaacgac gaggtcgtcc tgctcggggt cttcgccgac ttcgccgagc
     7321 tgagccgcaa ccgacccgtc ggggaggagg cgcacctgga gaaccgggtg cacagcccgc
     7381 gcgagcactt ccacacctac ctgcagacgc tggatcccga gcgaggcgca ctgcctgccg
     7441 acttcgtcgg tcgtctcgag cgtgtgctca gccactacgg ggtcacctcc gcctcgcgtg
     7501 acgacgccgg tgacgcggct gccgccgacg ccctcgaggg tgcggtgttc cgggtgttcc
     7561 tggcccagca gcgctctgct cccgaggtcg cgatggccac cgcgatcctg cagcgctggc
     7621 tgggtgagcc ggcacccgag ccgccggccg acgtcgccgc ccgtgagctg ctcgaccgcc
     7681 tcgtgagcgc cacccagctg cggtttccga tcgtcggaga cctcgcccgc agtgtgcgct
     7741 ttcgctggtt cgaccagccc ctcgtcgacg ccgaccgggc gtccgtgctc ggctccgtcg
     7801 gcgacgagct cgccgagatc gacgccctcc cggacgggcc cgagcgcacc gcccgcatcg
     7861 acaccctcgc cgcgatcccc gagcggatcg tgcggttcct cgccgagcgg ctgcgcaccg
     7921 gcatccaggc tcgtgagccg atgctcgccg tcctcgtcaa gcggcactac cgcgagtacg
     7981 acctcgtcga cctgcgcgag gagaccatcg acggtcgcgg cgtcgccagc gcgaactacg
     8041 ttctcgacaa ccgcgtctcg cacctcatca ccacgaccgg caccgtcgac gagctcaccg
     8101 acggcagccc gctgtcgacg ctgctgagtg gcctggtcgc ccagcgcccg gtcggtcacc
     8161 agggcgtcgt cgacctctac gtccactggg gcgacctgcc cgccgacaag gaggccgcct
     8221 ccgcagcgct ggccgagaag atcgcccgga tgccgttcgc ggcccaggtg cggcgcgtcg
     8281 ccgtgggcgt ggtccccgac gacgagcgcg aggtcggcta cttcacgttc cggccgcagg
     8341 gcgacggatc cctggtcgag gaccggccgg tgcgtgacgt gcaccccatg gtggggcgtc
     8401 ggctcaacct gtggcggctg cgcgacttcg acctcacccg catcgacgcc cccgaggacg
     8461 tcctgctcct gcacgcggtc gcgacgggca acccgcagga ccagcggctc gtggccctgg
     8521 cccaggtgcg ccagctcgtc gtcgtgcgcg acgctgccgg caaggtcacc ggcctgccgc
     8581 acatcgagcg ggcgctcgcg aactgcctcg aggcgatccg tcgggcccgg gccaccttgg
     8641 gcgccggggc gcggctggac accaaccacg tgtggctgca cgtctggccg cccgtcgagg
     8701 ccgacctcga ccagctgacg gcgctccagg gcaaggtcgc gccgatgacc gcaggtgcgg
     8761 gcatcgagga ggtgcgcgtc gagggtgcca tccccggcga ggacggcacc accgtgccga
     8821 tctgtgcccg cttcgcgtac cagcccgggt caggggtgac cttcaccgtc gaccagcccc
     8881 cgaccacccg cgtcgcaccg ctggacgagt acgccgagaa ggtcgtgcgg gcccggcgcc
     8941 gcgggttggt ctacccgtac gagctggtct cgatggtggc cggctccggt ggttcggtgc
     9001 aggagctcga cctcgacgag agcggtcggc tcgtgccggt cgagcggccc tacggcgaga
     9061 acaccgcggg cctgatctgc ggcctggtca cgaccccgac ccccctgcac cccgaggggg
     9121 tcagccgggt gctgctctgc ggcgacccgc tgcgggccct tggtgcggtg gccgagccgg
     9181 agtgcgcccg ggtcatcgcg gccctggacc tcgcggagga gtggggcctg ccggtcgagt
     9241 ggttcgccct gtccgccggt gcccgcatct ccatggactc gggcaccgag aacatggact
     9301 gggtcgccaa ggcgctcaag cggatcatcg agttcaccca ggccgggcac gagatcaacg
     9361 tcgtcgtcgc cggcatcaac gtcggggcgc agccgtactg gaacgccgag gcgacgatgc
     9421 tcatgcacac caagggcatc ctcgtcatga cgcccgactc ggcgatggtg ctcaccggaa
     9481 agcagtcgct cgacttctcc ggtggcgtct cggccgagga caacttcggc atcggtggct
     9541 acgaccgggt catgggcccc aacggccagg cacagtactg ggccaaggac ctcgtcgacg
     9601 cctacggcat cctcctcgcg cactacgaga acacctgggt cgcgccgggc gagagcggcc
     9661 cgcgccgggc cccgacgagc gacccgcacg accgtgacgt cacgacgcac ccgcacaccc
     9721 tcgcgggcag cgacttcacg accgtcggcg acatcttctc gtcggcgacc aaccccgacc
     9781 gcaagaagcc gttcgacatc cgcacgctga tgagggccgt cgccgaccag gaccacggga
     9841 cgctcgagcg ctgggccggg atggccgatg ccgagaccgc cgtcgtgctc gacgcccgca
     9901 tcggcggcat cccggtgtgc ctgctcggga tcgagtccaa ggcggtggcc cggcgtgggg
     9961 ttccgccgac tgacgggccg gacgtctaca cggccggcac gctcttcccg cgctcgtcga
    10021 agaaggcggc ccgggcgatc aacgcggcga gcggcaaccg gcccctcgtc gtgctggcca
    10081 acctgtcggg cttcgacggc tcacccgact cgatgcgcgc cctgcagctc gagtacggcg
    10141 ccgagatcgg ccggtcggtc gtgaacttcg acggcccgat cgtcttcgtc gtggtgtcgc
    10201 gctaccacgg tggcgcgttc gtcgtgttct ccaaagcgct caacccccgg atgacggtgc
    10261 tcgcggtgga gggctcgttc gcgtcggtca tcggcggggc cccggccgca gcggtggtgt
    10321 tcgcgcgcga cgtcgacaag cgcacggcag ccgacccgcg ggtcgccgac ctcgaggcgc
    10381 gggtcgccgc cgcgagcggg gccgaacggg cggcgctcgc gaccgagctg atcgcggtgc
    10441 gcaccgccgt tcgcgccgag aagctcgggc aggtggcgtc ggagttcgac cgggtgcacg
    10501 acatccaccg ggccgtgcag gtcgggtcgg tcgatgcggt catctcggcc tcggagatcc
    10561 gtcccaggat catcgccgcc atcgaggcca ccaccgcctg accccccttt cccgacttcg
    10621 tgcgacttga gtgcggctct gggccacaaa tcggcccaaa ccggtaccca agtcgcacga
    10681 agtcggagag ggtggggcgg gtgaccaccg tggggctgct gctcgccgct ggggccggcc
    10741 gccggatggg cggacccaag gccctgctgc gcctgacccc aacgggcccg accctcatcg
    10801 agacggccgt cgagcgcctg tggcaggccg gctgcgaccg ggtcgtcgtc gtcctgggtg
    10861 ccggggcgtc gcaggcgtcg gatgccgtgg cgcaccttcg cgtcgacgtc gtcaccgtgc
    10921 ccgactgggc cgagggcatg ggcgcatcgc tgcgtgccgg gctcgcagac ctcagctcgg
    10981 tggcacccca ggaggtggca gccgacatcg ccctcgtgac cctcgtcgac ctgcccgacg
    11041 tcacggccga ggtcatggcc cgggtcctcg ccgccgcgca accgcagggg gcagcggccc
    11101 tggtgcgggc ggcatacggc ggcgtgcccg gccacccggt cgccatcggg cgtgatcact
    11161 gggaagccgt gcgcaccacc gcccgaggtg accgcggcgc ccgcgaccac ctgcgcacca
    11221 ccgagcacga cctcgtcgag tgcggcgacc tcgccagcgg ccgcgacgtc gacaccgtcg
    11281 ccgacctccc cgggccccac cggtggcgcg acatgatggg gccgtgagcg caccagagca
    11341 ccgctggccc ggcagcgtct acgaccacgg cagcgacccc gatccccgct tctcgctggc
    11401 gaacgagcgc accttcctcg cctggatccg cacggcgctg gcgctcgtcg ccggagccgc
    11461 ggccgtcgat gccctcccgc tccccatgcc ggatgccgtc cagcgcgccc tcgctgccgg
    11521 gctggccctc gccgccctgc tcaccgcctt cgccgcctgg cgcgggtggg cagggaccga
    11581 gcgggcgatg cgccagggca agtcactgcc gtcgaacccg gcgatggtcg tcgtgctcgt
    11641 ggccgtggcg gtcactgccg tggtcctcgg catcacgagc gtgaccggtg cctgagacgg
    11701 atctgaccaa caagtcagag ctgtcccagt ggtggtgggc tccgggtggt gagccggccc
    11761 gtgggtgatc cggctgcccc acaggagcgc accaccctgg cgtggcgccg caccgggctg
    11821 gcgctgctca tcggagccct gacgatcggc cgcctcaccc tcgacgccct cggcccggcc
    11881 gtcgtcgtcc ccacagcgct ggctgctgcc ctcgcgggct gggtgatcat cgcggcgctg
    11941 cgcacccgcc ggctggcccg agcccacccc ggcgagcccg gtttctcggt gctcgccgac
    12001 ggccgcctgc ccgccgtcgt cgcggccctc gtcggggcac tggccgtcgg cgaactgctc
    12061 gccgctgtgg cccacctgct ctgaccacct gccctgcggc agcgcaagcc gaccacacgg
    12121 catccgtcgg ggaggggagt gcttccgatc gggcggcgcg tcagggatgc tggcgccatg
    12181 gacccccgca cgacgttcga ctcggcagcc gacctcgagg gcgcgctcgg cgcgacgggg
    12241 tacctcaccg acgaggccct ggccaccgtc acctggctgg cgctgcgcct cggccggccg
    12301 ctgctcatgg agggcgagcc cggcaccggc aagaccgccc tggccgaggc cctggcgcag
    12361 gccatggacc tgcccctcat ccgcctccag tgctacgagg gcatcgacgc cacgcaggcc
    12421 ctctacgact gggacttccc ccgccagatc ctccacgtgc gcgcgctcga agccgtcgag
    12481 gacggcccca gagacgtccg tgacgtcgag gaagggctgt tcgacgagcg cttcctgctc
    12541 gcccgccccg tcctgcgcgc cctgcgcgag tcgccggcgg tgctgctcat cgacgagatc
    12601 gaccgggccg acgacgagtt cgaggcgttc ctgctcgagg tgctctcgac gtggcaggtg
    12661 accatccccg aactcggcac ggtcacggca tccgtcccgc cggtcgtcat cctcacctcc
    12721 aaccgcaccc gcgagctgca cgacgccctc aagcgtcgct gcctctacca ctggctcgag
    12781 caccccggca tcgcgcgtga gctcgcgatc gtgcgcagcc gcgcccccga ggtgcccgag
    12841 gcgctcgccg accaggtcgt gcgactcgtc cacggcatcc gcaccgatcg cgagctgctg
    12901 aagccccccg gcgtcgccga gaccctcgac tgggcccgcg ccctgaacga actcggtacc
    12961 cgcgagctcg acaccgcgac ggcggccgcg accctgggcg ttgccgtgaa gtaccgcgag
    13021 gatgccgaac gcgttcgccg cgccctcgac atcatgctca cgacatgacg tcagatgccg
    13081 tccgcccggg ttcggatgcc gtccgc
//
LOCUS       DAOSOC010000052        13080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4776_length_13080_cov_11.323301, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000052 DAOSOC010000000
VERSION     DAOSOC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4776_length_13080_cov_11.323301"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..703)
                     /locus_tag="PL014_05830"
     CDS             complement(2..703)
                     /locus_tag="PL014_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT56645.1"
                     /translation="MNGASAAPVRVAIIDDDPLVRAGLAMILGGDPTLSVVGEAGDGA
                     DAVALVREVAPDVVLMDIRMPRRDGLAATGDLLALREPPKVLVLTTFDADEMVLAALR
                     VGAHGFLLKDTPPARLVEAVHAVAAGLPILSPHATSALIAEVATRDAAGPSSADRRRV
                     AARAALEALTERELEVAAALGRGLSNQEIGSALYLSVATVKAHVGRILTKLALENRTQ
                     VAILVHDADLGDDEN"
     gene            complement(700..1896)
                     /locus_tag="PL014_05835"
     CDS             complement(700..1896)
                     /locus_tag="PL014_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT56646.1"
                     /translation="MDTDGMTPPPPLRWWEHAWRLGLAALVSLVAFLVTVGPLAEADY
                     EPSGAEAFWILLADPVLGLAAFVLVLQRRRWPFVVAALTAAFSAVSLLALGPATLALA
                     SLATRRRPRELAPVVALTIAATVSMETLYPSIERESWWVGVLSGVLGGAVVIAVVVAI
                     GYAVGADREGRHTLRERAETNEREQQARVAAARAEERTRIAREMHDVLAHRISLVSMH
                     AGALTYRDDLPREQQVAVARTIEDNAQLAMSDLRTVLGVLRSDAAAEGMPEPPQPGTE
                     DLPALVAEAEVAGMRVLLDNQLTQAPPATTGRTLYRIVQECLTNARKHAPACSVTVSI
                     EGGPGEGVEVVVSNPLPVGAASPERLRGLGLLGLDERVALLGGSLDHGETGGRFVVRA
                     SLPWAA"
     gene            2039..2932
                     /locus_tag="PL014_05840"
     CDS             2039..2932
                     /locus_tag="PL014_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT56647.1"
                     /translation="MIRVENLTKRYGSVTAVDGATFTVRPGTVTGFLGPNGAGKSTVM
                     RMLTGLTPPTSGHATVLGRAYRDLPNAGSHVGVMLDASAQHPGRTGREALTLASIAIG
                     VPRTRVDEVLRLVGLTDDEAGRRVRTYSLGMRQRLGLAGAFLGEPRVLVLDEPANGLD
                     PQGIHWMRGLLRDFADRGGAVLLSSHLLAEVQAVADDIVVIGRGRIVAQGPVAELVDG
                     LGAIARSLDDAALVAALEAAGHAAAATASGVVVEAEPVDVARIALAAGIVVTDLRPHG
                     AQGLEDLFLRLTADDAREVAA"
     gene            2929..3738
                     /locus_tag="PL014_05845"
     CDS             2929..3738
                     /locus_tag="PL014_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56648.1"
                     /translation="MTTTMTPASAPTTPDAVHAPLEGSPGIPFRRLLEVERRKIVDTR
                     SGAWLLGITALLTALTLAVMLIWGESDGLGFMGLLSVATMPLSLLLPILGILTATTEW
                     SQRTALVTFTLEPRRGRVVVAKLLAVVGLSLTVLVLTILASALFYLASTTVRDGPADW
                     SYEPAVVAKLLLLLTIYLVQGLAFGLLFLNTPAAIVTSLLLPMVFTVVTTIVGSLERF
                     TAWVDLLVASEPLMAGSMGGAEWAHLGTASLLWLGLPFAVGTWRVLTKEIA"
     gene            3854..6718
                     /gene="topA"
                     /locus_tag="PL014_05850"
     CDS             3854..6718
                     /gene="topA"
                     /locus_tag="PL014_05850"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT56649.1"
                     /translation="MNPLAGRKLVIVESPAKARTIAGYLGPDWDVEASVGHIRDIPTP
                     SEMPAEMKKGPFGRFGVNIDADFEPFYVVDADKKKKVRELKALLKGADELYLATDEDR
                     EGEAIAWHLLETLQPTVPVKRMVFHEITKEAIQRAANTTRDVDTHLVDAQETRRILDR
                     LYGYEVSPVLWRKVKAGLSAGRVQSVATRLVVERERERMAFRSASYWDVEADVTPDTE
                     ANLFTARLTGVDGNRVATGRDFADDGTLTNAKAVQVDESAARGIADALTASDVVVTEV
                     SEKPYTRRPSAPFTTSTLQQEASRKLRLNSRNAMRVAQRLYENGYITYMRTDSTSLSD
                     AALSAARQQARELYGAEYVPDSPRRYEKKVKNAQEAHEAIRPAGDRFRTPAQVKGELR
                     GEEFALYELIWKRTVASQMADARGSTASVRLGATLPDGAPAASVELSASGTVITFRGF
                     LAAYEEGRDEKQTTKRKDEAQEERRLPRLGKGATLRTVRAEALGHETSPPPRYTEATL
                     VKAMEERGIGRPSTYASTIGTIQDRGYVSNRGSALIPSWLAFAVTRLMEEHFPRLVDY
                     GFTASMEEDLDAIARGDARRAEWLRRFYFGDEAKSSQGLRDLVEDLGDIDARAISTID
                     IGDGIVVRVGRYGPYIEEIVPAGIDPKTGEVTDAAALGEDGAPAAPRRATINDDVAPD
                     ELTPAMARELLATAADDGRVLGQDPDTGRDILAKAGRYGPYVTEVLPDEAPAAGATKT
                     RGKAVKAKPRTASLFKDMDLATIDLDTALKLLSLPRVVGADPESGEEITAQNGRYGPY
                     LKKGTDSRSLETEQQLFDITLEQALAIYAQPKQRGRAAAKPPLAELGNDPVSGRPVVV
                     KDGRFGPYVTDGETNATLRKDDDPEQISPERGYELLADKRARGPVKRTAKKAPAKKAT
                     TRKTAVKKTAVKKTATKSATKTAAKTTAKTATRKSTATKE"
     gene            6725..8056
                     /locus_tag="PL014_05855"
     CDS             6725..8056
                     /locus_tag="PL014_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT56650.1"
                     /translation="MSRVLVVTVPVPAHTRNALPIVARLVERGHEVAWFASRHFHDDV
                     EAVGATPVPYGRTRDFDGERLLEEFPQLAGLRGPRAIGRAYADVFVGEARNRIADLSA
                     LLAEQPVDVILCDGLSYGVSLLAEVHGVPYATIGDGPIAHAHGPTPAFGPGFQPMSGP
                     LSRLRNHAVRTATRRWILAQAQATHDALRADLGLAPDGRSVLEVSMSPLLHLQGCTPS
                     FEYPTPNMPASVRWIGALRPDPPANWMPPPWWDELLTSRRPVVHVTQGSIRPDMRELV
                     VPALRALADEDVLVVVTTGRVAEPDLVAALGAPLPANARVCEFVPYDLLLPHVDVCVA
                     NGGFTGVTTALHHGVPLVQVGSTEEKAEIGARIRWSGVGVRMRTTSPSPRRLRAAVRR
                     VLHDPGYRAAAQRIGVEMRSHDAGREGADLVERLCAPGAARGALAPTVTRQ"
     gene            8110..8562
                     /locus_tag="PL014_05860"
     CDS             8110..8562
                     /locus_tag="PL014_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOT56651.1"
                     /translation="MPLNPETLASLSPDGMFALIKGMSDADIRSDLAGEHRQEILEKV
                     FAHFPAQFLPDKAGDRNARIDFRITGGPGDSSDTYAVVVENGTCRIEPGASDAPDLSL
                     MLGPVEFFKLITGSGNPAMMFMMGKIKARGDIGLATALANWFDNTPKG"
     gene            8615..9406
                     /locus_tag="PL014_05865"
     CDS             8615..9406
                     /locus_tag="PL014_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT56652.1"
                     /translation="MTATSQHTASVDLTVGQVSQTFGVTVRTLHHYDAQGLVVPSART
                     HAGYRLYTDADLARLATVVTYRRLGLPLTEVRDLLDGQGTAAEHLRRQREVVLTRLGE
                     LTDLVSAIDAALEAEMNDRPATTEELKGLFGDGFSEDHQAEAEQRWGETTAWKQSASR
                     TKRYTPADWEQVKAEMDAVNAAFVAALTSGAAPDSDEAMAVAEAHREHIAQRFYDLDH
                     TFHRGLADMYVADPRFTKTYEDLAPRLAQYVHDAIHANADRHDVG"
     gene            9439..10083
                     /gene="tmk"
                     /locus_tag="PL014_05870"
     CDS             9439..10083
                     /gene="tmk"
                     /locus_tag="PL014_05870"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT56653.1"
                     /translation="MTGHDGLFIAFEGGDGAGKSTQVRLLAQALRARGRTVTVTRQPG
                     GTELGAAIRDLVLHGDHVSPRAEALLFAADKAHHVDQLVRPALERGEVVLTDRYTDSS
                     VAYQGAGRDLGAAEVHDLQMWAVRGLVPDLTVIVDITAAEGRRRRGGTHDRLESEADP
                     FHEAIRHHFLTMAAEHPDRYLVLDGTQPPERLHEQVLARLEGMGLVGADPGAAP"
     gene            10080..11219
                     /locus_tag="PL014_05875"
     CDS             10080..11219
                     /locus_tag="PL014_05875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOT56654.1"
                     /translation="MSVWRDVIGQEVAVATLEHAVTTPAAMTHAWLFTGPPGSGRSVA
                     ARAFAAALLCPDGGCGTCRECRTALEATHADVDVIATEGLTIKVEQARDLVQLAARSP
                     SVGRYRIIIVEDADRLTERAADALLKALEEPTPRTVWMLCAPSVEDVIITIRSRSRHV
                     RLRTPSVEAVADLLTRRDGVDPPMALYAARAAQSHVGLARRLARDEHARIRRHDVVAL
                     AAKISSVGDAVGAAGDLVTIATEESAAASSERDAAERVRLMEVLGADPSARTQPPHIR
                     SQLSSLEKEQKTRATRFTRDVIDRSLVDLLSVYRDALVLRTGAGTPLINEDSRQVVQR
                     VSEVFEAEALLRAMDAIGTARERIGANVNPLLALEAMALQLRLPR"
     gene            11346..12884
                     /locus_tag="PL014_05880"
     CDS             11346..12884
                     /locus_tag="PL014_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT56655.1"
                     /translation="MRRRRFAALAGVTAAALMASGCTIPGIGGSGDERTSVAPQTPPP
                     GAEGLSAFYSQQLAWSDCEGAQCATLTVPLDYAQPQGDTIEVSVIKVPAARESRRIGS
                     LVVNPGGPGGSGVDYARAADFIVGKGVRDAYDVVGFDPRGVGRSAPIDCLTDAELDTF
                     LGSDPTPDDPAEEQAFAKTAEGLGQSCANTAGPLLGHVSTQDAARDMDILRAALGEEK
                     LTYLGKSYGTYLGTIYADLFPAQVGRMVLDGVVAPDLTAEEINLGQAKGFERATREWA
                     AYCVEEGDCPLGTSVDEVMEGLRAFLLAVDSNPLPRTGDNAVPQLTEGWASLGIAAAM
                     YDQGQWRTLVDSMSEALKGDGTSLMQLANQYADRNPGGQYAGNIMEVIYAVNCLDKPE
                     PNDVAERARLAQESVADAPTWGPFLMWSSLPCGFWPIEPTGEPKTVAAQGAAPIVVIG
                     TTRDPATPYEWSVRLRDQLADASLITFDGDGHTAYTRSNDCVDKAVDAYYLKGTVPED
                     GFSC"
     gene            13006..13079
                     /locus_tag="PL014_05885"
     tRNA            13006..13079
                     /locus_tag="PL014_05885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13040..13042,aa:Thr,seq:cgt)
BASE COUNT         1945 a         4863 c         4508 g         1764 t
ORIGIN      
        1 gtcagttctc gtcgtcgccc aggtcggcgt cgtgaaccag gatcgccacc tgggtgcggt
       61 tctcgagcgc gagcttggtc aggatgcgtc cgacgtgggc cttgaccgtc gccacgctga
      121 ggtacagcgc gctcccgatc tcctggttgc tcagccctcg gccgagcgcg gcggccacct
      181 cgagttcacg ctcggtcagc gcctcgagag cagcacgggc agcgacccgg cggcggtcag
      241 cggaactcgg cccagcggca tcccgcgtgg ccacctcggc gatcagggct gacgtcgcgt
      301 gcggcgacag gatcggcagc ccggcagcca ccgcgtgcac ggcctcgacg agccgggccg
      361 gcggggtgtc cttgagcagg aacccgtggg cccccacccg cagcgccgcc agcaccatct
      421 cgtcggcatc gaaggtcgtc agcacgagca ccttcggtgg ctcacgcagt gccagcaggt
      481 cgccggtggc ggccaggccg tcgcggcgtg gcatccggat gtccatgagc acgacgtcgg
      541 gggcgacctc gcgaacgagc gccacggcat ccgccccgtc accagcctcg ccgacgaccg
      601 acagcgtcgg atcgcccccg aggatcatcg ccagcccggc ccgcaccagc gggtcgtcgt
      661 cgatgatggc gacccgcacc ggggccgcgg atgccccgtt catgccgccc acggtagcga
      721 cgcgcgcacg acgaagcggc caccggtctc gccatggtcc agcgacccgc cgagcagcgc
      781 aacgcgctca tcgagcccga gcagccccag cccgcgcagc cgctcggggc tcgcggcgcc
      841 gaccggcagc gggttgctca cgacgacctc gacgccttcg cccgggccgc cctcgatgct
      901 caccgtcacc gagcacgcag gggcgtgctt gcgggcgttt gtcaggcact cctggacgat
      961 ccggtagagg gtgcgccccg tcgtcgccgg cggagcctgc gtcagctggt tgtcgagcag
     1021 cacccgcatg cctgcgactt ccgcctcggc gaccagcgcc ggcaggtcct ccgtccccgg
     1081 ctggggtggt tcgggcatgc cttcggcagc ggcgtcgctg cgcagcaccc cgagcaccgt
     1141 gcgcaggtcg gacatcgcga gctgcgcgtt gtcctcgatc gtgcgggcga cggcgacctg
     1201 ctgctcccgc ggcaggtcat cgcggtaggt cagcgcgccg gcgtgcatcg acaccagtga
     1261 gatgcggtgg gccaggacgt cgtgcatctc gcgggcgatg cgggtgcgct cctcggcgcg
     1321 ggctgcggcg acgcgggcct gctgctcgcg ctcgttggtc tcggcgcgct cgcgcagggt
     1381 gtgccggccc tcgcggtcgg cgccgaccgc gtagccgatc gcgacgacga cggcgatgac
     1441 gacggcgccg cccaggacgc cggacaggac gccaacccac cacgactctc gctcgatgct
     1501 ggggtacagc gtctccatcg acacggtcgc cgcgatggtc agggcaacca cgggggccag
     1561 ctcacggggg cgtcgccgcg tcgccaagga cgccagggcc agggtcgccg ggccgagcgc
     1621 caacaaggag accgccgaga aggcagcggt gagcgcggcc accacgaagg gccagcggcg
     1681 acgctggagg acgaggacga aggccgcgag ccccagcacg ggatctgcca gcaggatcca
     1741 gaacgcctcg gccccgctcg gttcgtagtc ggcctccgcc aacggaccga cggtgacgag
     1801 gaacgcgacc aggctcacga gcgcagcgag tcccaggcgc caggcgtgct cccaccagcg
     1861 cagcggtggg ggtggcgtca tgccgtcagt atccatgccc cgacgctacg agcgcgccga
     1921 cctcgtgcgc gtcggctgaa ggtcgccccg gcagcgccga gacatagacc aaggtcgggg
     1981 gtggatgccg tccgctggcc gatgtgcgcc ccgcaccctc gcttgcaggc tggttcccat
     2041 gatcagagtc gagaatctca ccaagcgcta cggcagcgtc accgccgtcg acggcgccac
     2101 cttcaccgtg cggcccggga cggtcaccgg cttcctcggc cccaacggcg ccggcaagtc
     2161 aacggtcatg cggatgctga ccgggctgac cccacccacc agcggacacg ccaccgtgct
     2221 gggccgcgcc taccgcgacc tgcccaacgc cggctcccac gtcggcgtca tgctcgacgc
     2281 ctcggcgcag caccccggac gcaccggtcg cgaggcgctg accctggcct cgatcgccat
     2341 cggcgtcccg cgcacccgcg tcgacgaggt gctgcgcctc gtcgggctca ccgacgacga
     2401 ggcggggcgc cgcgtgcgca cctactccct cggcatgcgc cagcgactcg ggttggccgg
     2461 ggccttcctc ggggaacccc gggtgctcgt gctcgacgag ccggccaacg gcctggaccc
     2521 ccagggcatc cactggatgc gcggcctcct gcgcgacttc gccgaccgtg ggggagcggt
     2581 gctgctctcc tcgcacctgc tcgccgaggt gcaggccgtc gccgacgaca tcgtcgtcat
     2641 cgggcgcggc cggatcgtcg cgcagggacc ggtggccgag ctggtcgacg gcctcggcgc
     2701 catcgcccgc tcgctggacg atgccgcact cgtcgccgcg ctggaggccg ccggtcacgc
     2761 cgcggcggcc acggcatccg gcgtcgtcgt cgaggccgaa ccggtcgacg tcgcccgcat
     2821 cgccctcgcc gcggggatcg tcgtcaccga cctccgcccg cacggggccc agggcctcga
     2881 ggacctcttc ctcagactca ccgccgacga cgcccgggag gtggcggcat gaccaccacg
     2941 atgacccccg cttccgcacc caccacaccg gatgccgttc acgcacctct cgaggggagc
     3001 cccggcatcc ccttccgtcg gctcctcgag gtggagcggc gcaagatcgt cgacacccgc
     3061 tcgggagcgt ggctgctggg gatcactgcc ctgctgacgg cgctcaccct ggcggtcatg
     3121 ctcatctggg gggagtccga cggcctcggg ttcatggggc tgctcagcgt ggcgaccatg
     3181 ccgctcagcc tgctcctgcc gattctcggc atcctcacgg ccacgaccga gtggagccag
     3241 cggacggcgc tcgtgacctt caccctggag ccgcgacgcg ggcgggtcgt cgtggccaag
     3301 ctgctggccg tcgtgggcct ctccctgacg gtcctcgtcc tgacgatcct cgcgagtgcg
     3361 ctgttctacc tcgcgtcgac caccgtgcgc gacggccctg ccgactggtc gtacgagccc
     3421 gcggtcgtcg ccaagctctt gctcctgctg acgatctacc tcgtccaagg gcttgcgttc
     3481 ggtctgctct tcctcaacac cccggcagcg atcgtcacct cgttgctgct gccgatggtg
     3541 ttcaccgtgg tgacgacgat cgtcggctcc ctcgagcggt tcaccgcctg ggtcgacctg
     3601 ctcgtcgcca gtgaaccgct gatggcgggc tcgatgggcg gcgcggagtg ggcgcacctg
     3661 ggcacggcct ccctgctgtg gctggggctg ccgttcgcgg tcggcacgtg gcgggtgctc
     3721 accaaggaga tcgcctgacc ccgcgcagga ggggccgttt gtgcgtctcc cggggcgggg
     3781 cgctacggtc agggcgttcc cacgtcggag cagcgacgaa tccgctgcac gtggggaaac
     3841 ggaaacggaa caggtgaacc ccttggcagg acgcaagctc gtcatcgtcg agtccccggc
     3901 aaaggcccgc acgatcgcgg ggtacctcgg ccccgactgg gacgtcgagg cctcggtcgg
     3961 ccacatccgc gacatcccga cccccagcga gatgcccgcc gagatgaaga agggcccgtt
     4021 cggccgcttc ggtgtcaaca tcgacgccga cttcgagccc ttctacgtcg tcgacgccga
     4081 caagaagaag aaggtccgcg aactgaaggc gctgctcaag ggcgccgacg agctctacct
     4141 ggccactgat gaggaccgcg agggcgaggc catcgcgtgg cacctgctcg agactctcca
     4201 gcccactgtt ccggtcaagc gcatggtctt ccacgagatc accaaggaag ccatccagcg
     4261 cgccgccaac accacccgcg acgtcgacac ccacctcgtc gacgcccagg agacccgccg
     4321 catcctcgac cgcctctacg gctacgaggt cagccccgtg ctctggcgca aggtcaaggc
     4381 cggactgtcc gccggccgtg tgcagtcggt cgcgacccgc ctcgtcgtcg agcgcgagcg
     4441 tgagcggatg gccttccgct ccgccagcta ctgggacgtc gaggccgacg tcacccccga
     4501 caccgaggcc aacctgttca ccgcgcgcct caccggcgtc gacggcaacc gggtcgccac
     4561 cggtcgcgac ttcgccgacg acggcaccct gaccaacgcc aaggcggtcc aggtcgacga
     4621 gtccgcggcg cgcggcatcg ccgacgcgct cacggcatcc gacgtcgtcg tcaccgaggt
     4681 ctccgagaag ccgtacacgc gccgcccgag cgccccgttc acgacgagca ccctccagca
     4741 ggaggcctcg cgcaagctgc gcctgaacag ccgcaacgcc atgcgcgttg cccagcggct
     4801 gtacgagaac ggctacatca cgtacatgcg aaccgacagc acctcgctct cggatgccgc
     4861 gttgtcggct gcgcgtcagc aggctcgtga gctctacggc gccgagtacg tgccggactc
     4921 gccgcgccgc tacgagaaga aggtcaagaa cgcccaggag gcgcacgagg ccatccgccc
     4981 ggcgggtgac aggttccgca cgcccgccca ggtcaagggt gagctgcgcg gcgaggagtt
     5041 cgccctctac gaactgatct ggaagcgcac ggtcgcgtcc cagatggccg acgcgcgcgg
     5101 ctcgaccgcg tcggtccggt tgggggcgac cctgcccgac ggtgcgccgg cagcgtccgt
     5161 cgagctgtcg gcatccggca cggtcatcac cttccgcggc ttcctcgccg cgtacgagga
     5221 gggtcgcgac gagaagcaga cgaccaagcg caaggacgag gcgcaggagg agcgccgcct
     5281 tccccggctc ggcaagggcg ccacgctgcg caccgtgcgg gccgaggcgc tgggtcacga
     5341 gacctcgccc ccgccgcgct acaccgaggc caccctcgtc aaggcgatgg aggagcgcgg
     5401 gatcggccgc ccctcgacct acgcctcgac gatcggaacc atccaggacc gcgggtacgt
     5461 gtcgaaccgc gggtcggcgc tcatcccgag ctggctcgcg ttcgcagtga cccggctcat
     5521 ggaagagcac ttcccgcgcc tggtcgacta cggcttcacc gcctccatgg aggaggacct
     5581 cgacgccatc gcccgcggcg acgcccgccg cgccgagtgg ctgcgccggt tctacttcgg
     5641 cgacgaggcg aagtcctcgc agggcctgcg tgacctcgtc gaggacctcg gcgacatcga
     5701 cgcccgagcc atctccacca tcgacatcgg cgacggcatc gtcgtgcggg tcggccggta
     5761 cgggccgtac atcgaggaga tcgtcccggc ggggatcgac cccaagaccg gcgaggtcac
     5821 ggatgccgcc gcgctgggtg aggacggcgc acccgccgcg ccgcgccgcg ccaccatcaa
     5881 cgacgacgtc gccccggacg agctgacgcc cgcgatggct cgcgagctgc tcgcgaccgc
     5941 ggccgacgac ggccgggtac tgggccagga cccggacacc gggcgcgaca tcctcgccaa
     6001 ggcagggcgc tacggcccct acgtcaccga ggtgctgccc gacgaggctc cggccgcggg
     6061 ggcgaccaag acgcgaggca aggccgtcaa ggcgaagccg cgcacggcat ccctgttcaa
     6121 ggacatggac ctcgcgacca tcgacctcga caccgccctg aagctgctgt cgctgccgcg
     6181 cgtcgtcggt gccgaccccg agtcgggcga ggagatcacc gcgcagaacg ggcgctacgg
     6241 gccgtacctg aagaagggca ccgactcgcg ctcactcgag accgagcagc agctgttcga
     6301 catcaccctc gagcaggcgc tcgcgatcta cgcgcagccc aagcagcgcg ggcgggccgc
     6361 ggccaagccg ccgctggccg agctcggcaa cgaccccgtg tcggggcggc cggtcgtcgt
     6421 caaggacggc cggttcggcc cgtacgtcac cgacggcgag acgaacgcga ccctgcgcaa
     6481 ggacgacgac cccgagcaga tctcgcccga gcgcgggtac gagctgctcg ccgacaagcg
     6541 ggcccgcggg ccggtcaagc gcaccgcgaa gaaggcaccg gcgaagaagg ccacgacccg
     6601 caagaccgcg gtgaagaaga ccgcggtgaa gaagacggcc acgaagtccg cgaccaagac
     6661 ggcagcgaag accacggcga agacggcgac gcgaaagtcg accgcgacca aggagtgacc
     6721 cgcggtgtct cgtgtcctcg tcgtgacggt ccccgtaccg gcccacaccc gcaatgccct
     6781 gccgatcgtg gcgcggctcg tcgagcgcgg gcacgaggtg gcgtggttcg ccagccgcca
     6841 cttccacgac gacgtcgagg ccgtcggcgc gacgcccgtg ccgtacgggc gcacccgtga
     6901 cttcgatggg gaacggctgc tcgaggagtt cccccagctg gccgggctgc gcgggccccg
     6961 ggcgatcggc cgggcctacg cggacgtgtt cgtcggtgag gcccgcaacc gcatcgccga
     7021 cctgtccgcc ctgctggccg agcagccggt cgacgtcatc ctctgtgacg ggctctccta
     7081 cggagtgagc ctgctggccg aggtgcacgg ggtgccctac gcgaccatcg gggacgggcc
     7141 gatcgcgcac gcccacgggc cgaccccggc gttcgggccg gggttccagc ccatgtccgg
     7201 tccgttgtcc cggctgcgca accacgccgt gcgcacggcc acccgacgat ggatcctcgc
     7261 gcaggcgcag gccactcacg acgcgctgcg tgctgacctg gggctggcgc ctgatggccg
     7321 cagcgtgctc gaggtgtcga tgtcgccgct gctgcacctc caggggtgca ccccctcgtt
     7381 cgagtacccg accccgaaca tgccggcatc cgtgcggtgg atcggtgcgc tgcgaccgga
     7441 cccgccggcg aactggatgc ccccgccgtg gtgggacgag ctgctgacct cgaggcgccc
     7501 ggtggtccac gtgacacagg gcagcatccg gccggacatg cgcgagctgg tcgtgccggc
     7561 cctgcgcgcc ctggccgacg aggacgtcct cgtcgtcgtc accacggggc gtgtggccga
     7621 acccgacctc gtcgccgctc tgggggcgcc gttgcccgcg aacgcccggg tgtgcgagtt
     7681 cgtcccctac gacctgctcc tgccacacgt cgacgtgtgc gtcgccaacg gcggattcac
     7741 gggggtcacg accgcccttc accacggggt gcccctggtc caggtcgggt cgaccgagga
     7801 gaaggccgag atcggtgccc ggatccggtg gagcggtgtc ggggtgcgga tgcgcacgac
     7861 gagcccgtca ccgcgccggc tgcgtgccgc tgtgcgtcgg gtgctgcacg acccggggta
     7921 ccgtgccgcc gcgcagcgca tcggggtgga gatgcgcagc catgacgcgg ggcgcgaggg
     7981 tgccgacctc gtggagcgac tctgcgcccc gggggcggcg cgtggcgccc ttgccccgac
     8041 ggttactcga cagtaggttc tggggacagc ccgggccgtc catgaccggg gcacgacccc
     8101 ggagttgtca tgccgctgaa ccccgagacc ctcgcatcgc tgtcgcccga tggcatgttc
     8161 gccctcatca agggcatgtc cgacgccgac atccgctccg acctcgccgg tgagcaccgt
     8221 caggagatcc tcgagaaggt cttcgcgcac ttccccgcgc agttcctccc cgacaaggcc
     8281 ggcgaccgca acgcgcggat cgacttccgc atcaccgggg gcccgggcga cagcagcgac
     8341 acctatgcgg tcgtcgtcga gaacggcacc tgccgcatcg aaccgggcgc gagcgacgca
     8401 cccgacctgt cgctcatgct cggccccgtg gagttcttca agctcatcac cgggtccggg
     8461 aacccggcaa tgatgttcat gatgggcaag atcaaggccc gtggggacat cggtctggcc
     8521 accgccctgg cgaactggtt cgacaacacc ccgaagggct gacctgcggc atccggcttg
     8581 accctcacgc gacgtgaggc gtcatggtgg tgacatgacc gcaacgagcc agcacactgc
     8641 atccgtggac ctcacggtgg gccaggtgtc acagaccttc ggcgtcacgg tgcgcaccct
     8701 gcaccactac gacgcgcagg gtctcgtcgt gccgagcgcg cgcacccacg ccgggtaccg
     8761 cctctacacg gacgccgacc tggcccggct cgcgaccgtc gtcacctacc gtcggctcgg
     8821 cctgccgctc accgaggtgc gcgacctgct cgacgggcag gggacggcag ccgagcacct
     8881 gcgacgccag cgcgaggtgg tcctgacccg gctgggcgag ctcaccgacc tcgtctcggc
     8941 catcgacgca gcactggagg cagaaatgaa cgaccgaccc gcgacgaccg aggagctcaa
     9001 gggcctgttc ggcgacggct tctccgagga ccaccaggcc gaggccgagc agcgctgggg
     9061 tgagaccacc gcctggaagc agtcggcgtc acgcaccaag cgctacaccc cggccgactg
     9121 ggagcaggtc aaggccgaga tggatgccgt caacgcggcg ttcgtggccg ccctcacctc
     9181 gggtgccgcc cccgactcgg acgaggcgat ggctgtggcc gaggcgcacc gcgagcacat
     9241 cgcgcagcgg ttctacgacc tggaccacac gttccaccgc ggcctggccg acatgtacgt
     9301 cgccgacccg cggttcacca agacctacga ggacctcgcg ccgcggctcg cgcagtacgt
     9361 tcacgacgcg atccacgcca acgccgaccg gcacgacgtg ggctgagcct gtcgccgcac
     9421 tggcctaggc tcgcctgcgt gacaggacac gacgggttgt tcatcgcctt cgagggtggc
     9481 gacggcgcgg gcaagtcgac ccaggtgcgg ctgctcgcgc aggcgctgcg tgcccgcggg
     9541 cgcacggtca cggtcacccg ccagcccggc ggcaccgaac tcggtgcggc gatccgcgac
     9601 ctcgtcctgc acggtgacca cgtctcaccg cgggccgagg ccctgctctt cgccgcggac
     9661 aaggcccacc acgtcgacca gctggtccgc ccggcgctcg agcggggcga ggtggtgctg
     9721 accgaccgct acaccgattc ctccgtcgcc tatcaggggg cggggcgcga cctcggcgcg
     9781 gcagaggtgc acgacctgca gatgtgggcg gtgcgcgggc tcgtgcccga cctcacggtc
     9841 atcgtcgaca tcaccgctgc cgagggccgc cgccgccggg gcggcaccca cgaccggctc
     9901 gagtccgagg ccgacccctt ccacgaggcc attcgccacc acttcctcac gatggcggcc
     9961 gagcacccgg accgctacct cgtcctcgac ggcacgcagc ccccggagcg cctgcacgag
    10021 caggtgctcg cccgactcga ggggatgggc ctcgtgggcg cagacccggg tgctgcgccg
    10081 tgagcgtctg gcgagacgtc atcggtcagg aggtggccgt cgcgacgctg gagcacgcgg
    10141 tgacaacccc cgcggcgatg acccacgcct ggctgttcac cggcccgccc gggtcgggtc
    10201 gctcggtggc ggcccgcgcc ttcgctgccg ccctgctctg cccggacggt ggctgcggca
    10261 cctgtcgcga gtgccgcacg gcgctggagg ccacccacgc cgacgtcgac gtcatcgcga
    10321 ccgaagggtt gacgatcaag gtcgagcagg cgcgcgacct cgtgcagctc gccgccagga
    10381 gcccgtccgt cggccgctac cggatcatca tcgtcgagga tgccgaccgc ctcaccgagc
    10441 gcgcggccga cgcactgctc aaggctctcg aggagcccac tccccggacg gtgtggatgc
    10501 tgtgcgctcc ctccgtcgag gacgtcatca tcacgatccg ttcgcgctcg cggcacgttc
    10561 gcctgcgcac cccgtcggtc gaggccgtcg ccgacctgct cacccggcgc gacggggtcg
    10621 acccgccgat ggcgctgtac gccgcgcggg ccgcccagtc gcacgtcggt ctggcccgca
    10681 ggctcgcccg tgacgagcac gcgcgcatcc gtcgccacga cgtcgtggct cttgcagcga
    10741 agatctcgag cgtcggggat gccgtggggg cggccggcga cctcgtcacc atcgcgacgg
    10801 aggagtcggc cgcggcctcc agcgagcggg acgccgcaga gcgggtccgg ctgatggagg
    10861 tgctcggggc cgacccgtcg gcacgcaccc agccgccgca catccgctcg cagctgtcgt
    10921 cgctggagaa ggagcagaag acccgggcca cgcgcttcac ccgcgacgtc atcgaccgct
    10981 cgctggtcga cctgctgtcg gtgtaccgcg acgcgctcgt gctgcgcacc ggcgccggga
    11041 cgccgctcat caacgaggac agtcgtcagg tggtgcagcg agtcagcgag gtcttcgagg
    11101 ccgaggccct gctgcgggcc atggacgcga tcggcacggc ccgcgagcgc atcggcgcca
    11161 acgtcaaccc gctcctggcc ctcgaggcga tggccctgca gctgcggctg ccccgctgag
    11221 gcgacccgcc ggcatccgac cccctgccag caggactcga tggcggtcac ccgacgtgcc
    11281 gccgcgaccg gatcgtgacc tgctcgattc cgccctgcgc agcttgctcg cttatcgtgc
    11341 gagccatgag acgccgtcgg ttcgctgccc ttgccggtgt cacggccgcc gccctgatgg
    11401 cgtccggctg cacgatcccc gggatcggcg gctccggcga cgagcgcacc tcggtggcgc
    11461 cgcagacccc accgcccggt gccgaagggc tctccgcctt ctacagccag cagctcgcgt
    11521 ggagcgactg cgaaggggcg cagtgcgcca cgctcacggt tccgctcgac tacgcgcagc
    11581 cgcagggcga caccatcgag gtctcggtca tcaaggtccc ggcagcgcgt gagtcgaggc
    11641 ggatcggctc gctcgtcgtg aacccgggtg gccccggcgg atccggggtg gactacgcgc
    11701 gggcggctga cttcatcgtc ggcaagggag tgcgcgacgc ctacgacgtc gtcggcttcg
    11761 acccgcgcgg ggtcggccgg tcggctccga tcgactgcct caccgacgcc gagctcgaca
    11821 ccttcctcgg gtcggacccc accccggacg acccggcaga ggagcaggcg ttcgccaaga
    11881 ccgccgaggg cctgggccag agctgcgcca acactgcggg gccgctgctg gggcacgtgt
    11941 ccacgcagga cgccgcacgc gacatggaca tcctgcgagc ggcgctcggc gaggagaagc
    12001 tcacctacct cggcaagtcc tacggcacct acctcggcac catctacgcc gacctcttcc
    12061 cggcgcaggt cggccggatg gtgctcgacg gtgtcgtcgc cccggacctc acggctgagg
    12121 agatcaacct cggtcaggcc aagggcttcg aacgagccac ccgcgagtgg gctgcgtact
    12181 gcgtcgagga gggggactgc ccgctcggga cctccgtcga cgaggtcatg gaggggctgc
    12241 gcgccttcct gctcgcggtg gacagcaacc cgctgccgcg caccggcgac aacgccgttc
    12301 cccagctcac cgagggctgg gcctcgctgg gcatcgctgc cgccatgtac gaccaggggc
    12361 agtggcgaac cctcgtggac tcgatgtccg aggcgctcaa gggcgacggc acctcgctca
    12421 tgcagctcgc gaaccagtac gcggaccgca accccggggg ccagtacgcc ggcaacatca
    12481 tggaggtcat ctacgcggtc aactgcctcg acaagccgga gccgaacgac gtggccgagc
    12541 gggcccgcct ggctcaggag tcggtggcgg acgcgcccac gtgggggccg ttcctcatgt
    12601 ggagctcgct gccgtgcggg ttctggccga tcgagcccac cggtgagccg aagaccgtcg
    12661 cggcgcaggg tgcggcgccg atcgtcgtca tcggcacgac ccgtgacccc gccaccccct
    12721 acgagtggtc ggtgcggctg cgcgaccagc tcgccgacgc ctcgctcatc accttcgacg
    12781 gtgacgggca cacggcctac acgcgctcga acgactgtgt cgacaaggcg gtcgatgcct
    12841 actacctcaa gggcaccgtg cccgaggacg gcttttcctg ctgacgaggt gggatgccgt
    12901 ccgggctggt tggtcctccc tcgcgccctg agcaggggcg ggattcgcgc cgaccgcggt
    12961 cgactcgtat actcgtgcgg cgtgcctgag cctgctcggg cgcacgccgc cttagctcag
    13021 tcggccagag cgattcactc gtaatgaata ggtcatcggt tcgattccga taggcggctc
//
LOCUS       DAOSOC010000053        12997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4816_length_12997_cov_8.954180, whole genome shotgun sequence.
ACCESSION   DAOSOC010000053 DAOSOC010000000
VERSION     DAOSOC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4816_length_12997_cov_8.954180"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..2017
                     /gene="arsA"
                     /locus_tag="PL014_05890"
     CDS             134..2017
                     /gene="arsA"
                     /locus_tag="PL014_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HOT56656.1"
                     /translation="MAEARAVELTCVEQAARVDESLTRLTFLEAPPRFLFFTGKGGVG
                     KTSLACASAVRLTTRGARVLLVSTDPASNIGQVFGLPVGNTRTPIPDVPGLSALEIDP
                     QQAADAYRARIIDPVRTQLPPDEVDAMTEQLSGACTTEIASFSEFTSLLANPDVTAGY
                     DHVLFDTAPTGHTIRLLQLPGEWTTYLSDGKGDVSCLGPVAGLDRFRHDYRGALDALT
                     DPTRTRLVIVSRPQRSALDEASRTYAELLRVGMSDAHLVLNGGMPPVDATDPLACAVR
                     AREAAALTGMPANLRPLPQTTTPLMPFNTVGVAALKALLEPSRAPLPDTADDHHPPVP
                     PEGFVDLRSLIDGLEAAGPALVMCMGKGGVGKTTIAAAIALELAHRGNDVLLTTTDPA
                     AHLTDTLDGEHENLTVSRIDPAHAISEYRERVMAGKGASLDDAGRAALAEDLKSPCTD
                     EVAVFQQFSHVVFQSRRRFVVLDTAPTGHTLLLLDATGSYHREIARQMSDGTRYTTPL
                     MRLQDPAQTKVLLITLPETTPVQEAEELQGDLARAGITPYAWVVNNSLVAAAPTSPFL
                     QARARAERPSLNRVAGLCARVGIVPMLTREPVGAASLTALASASVPGPWSPESGGPGP
                     RRQ"
     gene            2031..4562
                     /locus_tag="PL014_05895"
     CDS             2031..4562
                     /locus_tag="PL014_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOT56657.1"
                     /translation="MSSSPPGARPRSVTIVAVLAALLALADIVSGVVVVLGAPSADPL
                     GGMTQEQSVLTLRVVGGWFIALGVAQAVIAWLITRRSTAARLALSVLLVLRLAQSWFL
                     VSQLGTEAIQGFWGLALAVLLVYLLWNSAASRWFDGGRARTLADSLDDDPGTSAPSGA
                     RVLDFISRLAVLGATIALMPGVAASSSFSLLAAVVMIALVGWVLQPVFLRIALVFGWF
                     GAVLLALFANALVIGLGLYLTPGITVASPLSAFFAAWVYAFVITLLTWAFSINGRDYL
                     TVHAVRMASRGARPEPSDVPGVIFIQLDGVPAPLLENEIRAGNIPTISRWVRSGSHTW
                     TEWTARVPSTTPVSQAGLLHGHNDGIPAFRWFDRDLGRLLVANRPADAAVIEARISDG
                     RGLLADDGVSISNLFSGDAPTSLLTMSGLKQGRAGLGPSQSYAAFFTHPAGLLRAVIL
                     TIGEMGKEIFQARRQVRRGVEPRIHRGGAYVALRAATNVFLRDLNVALVVEAMMRGSK
                     SIYVDFVDYDEIAHHAGVTRSESLAAFYGLDDALRSIEQIIDSGVAPRPYEIVLVSDH
                     GQSQGATFRQRYGQTLEEVIREHLADGQSVAAATDEVETWGPVNMLLGQLSGQDSVSG
                     RLTKKVLSDRDPQAPVGPAGSGGPVGGDATSDAGPVETPADLTVVGSGNLGGVWFSQH
                     PTRLTAEDIERLHPGLLSALAAHPGIGFVVVLTAGGPVALGANGTHDLTTGVVVGDDP
                     LAPFGPDAVSDFVHVSGFPNAPDIYLNSLYDPVLDEVAAFEELVGCHGGLGGWQTRPL
                     LVHPAGWTVDEDLLDERGRLRGADTVHHQLVRWLERLGHRENLAA"
     gene            4896..6944
                     /locus_tag="PL014_05900"
     CDS             4896..6944
                     /locus_tag="PL014_05900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT56658.1"
                     /translation="MLARTVRQVEAAAQRGKVRPSQRTAYQVVALLVREERARVKADT
                     TISESERTAMLTRLEGIATILAKTAARDTSLLELLTDGAVVSSAAIDLKNDLLRAAGR
                     EPEPEPTPVVSPTAPFASASPRGVVPKTVIARQLANPFLAPDFSALPAPKPRTNRLGG
                     WELLSPLFRAFEYGGDATCMPLPEPTRVVHGIKGLELMHHQAQVVAAAAEGHRTFLLA
                     DEPGLGKTAQALLAADAAGAFPLLVVVPNVVKANWAREAELWTPRRAATVIHGDGDTV
                     DGFADIVIVNYEILDRHVGWLGAHGFRGMVVDEAHFIKNKSSQRSQHVLELSERIRSK
                     HARALFMALTGTPLINDIEDFKAIWQFLGWIDSTKPRHALMAALEETGLTPADHGFYA
                     AARTSVINQGIVRRRKVDVASDIPARRVADLPVELDDEASRSIRAAEQELAARLVKRY
                     DAALAARSVSVVVEGIDHDMVRKVAQWERKNATTATTGDNVFTVMRKIGQAKAGLAAD
                     YAAQLARNVGKVVFFAKHIDVMDIAEQTLAKRGIGYASIRGDQTPAVRKANIEAFLTD
                     PEISVVVCSLTAAGVGLNLQVASNVVLAELSWTSAEQTQAIDRVHRIGQTEPVTAWRI
                     IAAQTIDPRLAQLIDDKAGLAARALDGSDEEVGSSADLQLEALMAMLTDALEARGFPA
                     "
     gene            complement(6945..10490)
                     /locus_tag="PL014_05905"
     CDS             complement(6945..10490)
                     /locus_tag="PL014_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HOT56659.1"
                     /translation="MLLLGDDARLVISASDLRTASACEFAVVRQLDVLLGRAERVEEP
                     RDPMLDRVKALGDQHEQAELRRLSREHPGRVRHLPTPSNTPHDLAAAMTTTLETLSSG
                     AEVVSQATVFDGGFVGRADFLELTPAGWLVSDTKLARHATVSALLQVGAYAALLLDAG
                     APVAPIVRLVLGDGATRDDPLGEVLPVYRSRRARLDGILADHRADEAPAAWGDERWLA
                     CGSCATCVAEVEAARDLLLVAGMRRPTRRRLHEAGVRTIDDLASSTGPVADVRAQSLD
                     RLRAQARLQLVQEADPTGAVHHEVTDVAALSRLPAPSTGDIFFDFEGDPLWQEPGSGI
                     WGLEYLFGMIEVDSGEERFRAFWAHDRREEKQALVSFVDHVNARLATWPDLHVYHYAP
                     YEPAALLRMAARHGVYEDEVDDLLRRRVFVDLYAVVKAGVRISQRSYSIKKLEPLYMP
                     AREGEVQGGAESIVVYHQFSAATNEGRTDEAASLLAEIADYNELDCVSTLRLRDWLLA
                     RCRDAGGSPAGPVDDDGASAAPVSDKRAAALALEAAVRALVDDIPPAQWSDEQRAMAM
                     IASSVLYHAREDKPAWQEHYERLRLPVGDWRRADGVFVVDRAEVVEPWAQQSARHNPR
                     RVLRLTGEAAGGAPRVGECHVVYAAPGPAGFEAPPMDANTVNPSKGTVLSIDEELSAD
                     GVARLVVTLSEQHTRSLGDHDDLPVALTPGGPIKTDSIDRALAEVAEEVLASGAVPVS
                     AGTDVLLRRAPRLRGGAALPPVADAGDDRVINAIEAALLAMDDSYVAAQGPPGTGKTH
                     VGGHVIARLAAWGWRIGVCAQSHAAVENVLSAVVRAGLDPAQVGKVAKATPDPAWTSL
                     PKADDLAAFAAQHDQVGRGYVIGGSAWDLTNERRVARDQLDLLVIDEAGQFSVAKTLA
                     VGVCARRLLLLGDPQQLPQVSTGVHAQPVDESALGWLAAGEPVLPAHLGYFLETTRRM
                     HPDLTTVVSDLAYDGALTSYEQVTAARRLDGIRPGLHVVVVDHLDNSTESLEEAEAVC
                     SLAQTLIGRMWHDPSATDGPRAPAPGGPGAPAPGRPLTARDLIVITPYNRQADRIRAV
                     LRGAGLEDVRVGTVDKFQGQEAPVAILSMAASSHSEVSRGMGFLLDRHRLNVGISRGQ
                     YAAYVVRSRVLTDFSPGSPDELIALGAFLRLHPRP"
     gene            complement(10608..12179)
                     /locus_tag="PL014_05910"
     CDS             complement(10608..12179)
                     /locus_tag="PL014_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOT56660.1"
                     /translation="MNRTDELRLRLLGPRPVSLMTDKLWGWLGPGLIALVGGFLRFWN
                     LDHPRAMVFDETYYVKQAASMLRFGVEMRVLSTLKEPDKVFTGGNPDVFSTVDGDMVV
                     HPPVGKWMLAAGQWVFGSDSGFGWRVSAAVVGTLSILILGRVARRMFGSTALGCVAAF
                     LLAFEGSHFVMSRTGILDIFVSFFALCGFAALLIDRDRSREILAARVGALPRGTWPQG
                     MGPWLGWRPWRWVAGLSLGLATSTKWSGLFFLAAFGLMTVWWDMGARRAAGVRRWGLG
                     AVLKDGPFAALAMVGTTAVVYVASWIGWFRSDVGYHRQWNTFHPGEGVMWLPPVLRNW
                     VKYHQDAFAFNTTLQTPHPYQSDPWTWILQLRPTSFYYESPTRGVAGCTVDQCSQAIN
                     PVGTITVWWLGVLALAVVVWWWLVRRDWRAGAIAAGIVGGYLPWFAFADRTIYAFYAV
                     AFEPWVVLAVTFVIALFVGRREDDPQRWRTRWFIVGGYLLLTLAMFAFFHPIYVGETI
                     PYDHWRWRMWLPSWI"
     gene            12265..>12997
                     /gene="rsmI"
                     /locus_tag="PL014_05915"
     CDS             12265..>12997
                     /gene="rsmI"
                     /locus_tag="PL014_05915"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT56661.1"
                     /translation="MSDHSGSSAGSGASSGGTGGSGVLVLAATPIGDPRDAAPRLAEE
                     LRTADVVAAEDTRRLRRLCQALEVEPTGRVLSYHEHNEAQRTPELVEQLLAGRRVVVV
                     TDAGMPSVSDPGYRLVSAAVAGGVRVTCVPGPSAVLMALAVSGMPVDRFCFEGFLPRR
                     AGERRTALGVLGDERRTMVFFEAPHRLERTLTTMAEVFGAQRRAAVCRELTKTYEEVR
                     RGTLAELVTWAADGVRGEITVVVEGA"
BASE COUNT         1915 a         4727 c         4488 g         1867 t
ORIGIN      
        1 tccaccgaca gcagaaggac ccagcatgct ttttgctggg tccttctgct ttgcgttgtt
       61 gacgggggtc tcgtcggcgt cagcctgctc acaggctgcg cgtcacgcgg tggccggtcc
      121 gtacgattga cctgtggctg aggcacgcgc ggtggaactg acctgcgtgg agcaggccgc
      181 gagggttgac gaatccctga cccggctgac gttcctcgag gcacctccac ggtttctctt
      241 cttcaccggc aagggtggcg tcggaaagac gtccttggcc tgcgcgagcg ccgtgcggct
      301 cacgacccga ggcgcgcggg tcctgctcgt gagcaccgac ccggcatcca acatcggaca
      361 ggtcttcggt ctcccggtcg gcaacacgcg cacgccgatc ccagacgtcc ccggcctgtc
      421 tgctctggag atcgatccgc aacaggctgc cgatgcctac cgggcccgga tcatcgaccc
      481 cgtgcgcacc cagctgcctc ccgacgaggt ggacgccatg accgaacagc tgtcgggtgc
      541 gtgcaccacc gagatcgcgt ccttcagcga gttcacctcc ctcctcgcga acccggacgt
      601 gaccgctggc tacgaccacg tcctgttcga caccgccccc accggccaca cgatccggct
      661 ccttcagctg cccggcgagt ggaccaccta cctctccgac ggcaagggcg acgtctcgtg
      721 cctcggcccg gtcgcgggcc tggaccggtt ccgacacgac taccgcggag ccctggacgc
      781 cctgaccgac cccacccgca cccgtctggt catcgtcagt cgcccccagc ggtccgctct
      841 cgacgaggcc tcccgcacct acgccgagct gcttcgggtc gggatgagcg acgcccacct
      901 cgtcctcaac ggtggcatgc cccccgtgga cgccactgac ccgctcgcgt gtgccgtcag
      961 ggcccgggaa gcggctgcac tcacggggat gccagccaac ctgcgacctc tcccacagac
     1021 cacgaccccc ctgatgccgt tcaacaccgt cggtgtggcg gcactgaagg cgctgctgga
     1081 accgtcccgc gcaccgcttc cggacaccgc cgacgaccac cacccacccg tgcctcccga
     1141 gggattcgtc gacctgcgct ccctcatcga cggcctcgag gcagccgggc cggcgctggt
     1201 catgtgcatg ggcaagggcg gcgtcggcaa gacgaccatc gccgccgcca tcgcgctcga
     1261 gctggcgcac cggggcaatg acgtgctgct gacgacgacc gatccggcgg cccacctgac
     1321 cgacaccctc gacggcgagc acgagaacct cacggtctcc cgcatcgacc ccgcacacgc
     1381 catctccgag taccgggaac gggtcatggc aggcaagggc gcctcgctgg acgacgccgg
     1441 cagggccgcg ctcgccgagg acctgaagtc gccctgcacc gacgaggtcg ccgtcttcca
     1501 gcagttctcg cacgtggtgt tccagtcccg caggaggttc gtcgtcctcg acaccgcgcc
     1561 caccgggcac accctgttgt tgctcgatgc gaccggctcc taccaccgcg agatcgctcg
     1621 gcagatgagc gacgggacgc ggtacaccac tccactgatg cgcctccagg accccgccca
     1681 gaccaaggtt ctcctcatca ccctgcccga gacgaccccg gtccaggagg ccgaggagct
     1741 gcaaggcgat ctcgcgagag ctgggatcac cccgtacgcc tgggtggtga acaacagcct
     1801 cgtggcggcc gcgcccacgt cacccttcct gcaggcgcga gcccgggccg agcggccgtc
     1861 cctgaatcgg gttgccgggc tctgtgcccg ggtggggatc gtgccgatgc tcacccgcga
     1921 gccggtcggg gctgcttccc tgactgctct cgcgtccgct tctgtgccgg gcccgtggtc
     1981 acccgagtcg ggcggacccg ggccgcggcg acagtgacag gctcgttcgc atgagctcga
     2041 gcccacccgg cgcgcgcccc cgttcggtga cgatcgtcgc tgtgctggcg gccttgctgg
     2101 cgctggccga catcgtgtcc ggggtcgtcg tggtgctcgg ggcacccagc gcggaccctc
     2161 tcggagggat gacccaggaa cagagcgtcc tgaccctgcg cgtggtcggg ggctggttca
     2221 tcgcgctcgg ggtggcgcag gcggtcatcg cctggctcat cacacggcgg tcgaccgcgg
     2281 cccggctggc gctgagcgtg ctgctcgtgc tccggctggc gcagtcgtgg ttcctcgtct
     2341 cccagctcgg aaccgaggcc atccagggat tctgggggct ggcgctggcg gtgctgctcg
     2401 tctacctgct gtggaactcg gcagcctctc ggtggttcga cggcggtaga gcacgcaccc
     2461 tggccgactc cctcgacgac gaccccggca cctcagcgcc atcaggtgcc cgggtgctcg
     2521 acttcatctc ccgcctcgcc gtgctggggg cgaccatcgc cctcatgccg ggtgtcgcgg
     2581 catcctcatc gttctccctc ctcgccgccg tggtcatgat cgcgctcgtc gggtgggtgc
     2641 tgcagccggt gttcctgcgg atcgccctgg tcttcgggtg gttcggcgcg gtgctgctcg
     2701 ccctgttcgc caacgccctc gtcatcgggc tcgggctcta cctcacgccg ggcatcacgg
     2761 tggcgagccc gctcagtgcg ttcttcgccg cgtgggtcta tgcgttcgtc atcacgctgc
     2821 tcacctgggc attcagcatc aacgggcgtg actacctcac ggtgcacgct gtgcggatgg
     2881 cgtcgcgcgg ggcacggcca gagccctccg acgtgcccgg ggtcatcttc atccagctcg
     2941 acggtgtgcc ggcgccgctg ctggagaacg agatccgcgc cggcaacatc cccacgatca
     3001 gccggtgggt gcgctcgggc agccacacgt ggaccgagtg gaccgcccgc gtcccctcga
     3061 cgaccccggt gagccaggcc ggcctgctcc acggtcacaa cgacggcatc cccgccttcc
     3121 gctggttcga ccgcgacctc gggcgcctcc tcgtcgccaa ccgcccggcg gatgccgcgg
     3181 tgatcgaggc gcgcatcagc gacggtcgcg ggttgctcgc cgacgacggc gtgagcatct
     3241 ccaacctgtt ctccggcgac gccccgacgt cgttgctcac gatgagcggg ctcaagcagg
     3301 gcagggcagg gctggggccc tcacagtcgt acgccgcgtt cttcacgcac ccggccggcc
     3361 tcctgcgggc ggtcatcctc accatcggcg agatgggcaa ggagatcttc caggcccgac
     3421 gccaggtgcg ccggggagtc gagccgcgca tccaccgggg aggggcgtac gtcgccctgc
     3481 gcgcggccac caacgtgttc ctgcgtgacc tcaacgtcgc gctggtcgtc gaggcgatga
     3541 tgcgcgggtc gaagtccatc tacgtcgact tcgtcgacta cgacgagatc gcccaccacg
     3601 ccggggtgac ccgctcggag tcgctcgccg ccttctacgg cctcgacgac gcgctgcgca
     3661 gcatcgagca gatcatcgac tcgggggtgg cgccgcggcc ctacgagatc gtgctcgtct
     3721 ccgaccacgg gcagagccag ggggcgacgt tccgccagcg ctacggccag accctcgagg
     3781 aggtcatccg cgagcacctc gccgacggcc agagcgtcgc cgcggcgacc gacgaggtgg
     3841 agacgtgggg gccggtcaac atgttgctcg ggcagttgtc ggggcaggac tcggtgtccg
     3901 ggcggctcac caagaaggtg ctgtcggatc gggatccgca ggcacccgtg ggccctgccg
     3961 ggtcgggcgg cccggtcggg ggcgacgcca cgtcggatgc cggccccgtc gagacccccg
     4021 cggacctcac cgtcgtcggg tcgggcaacc tcggtggggt ctggttcagc cagcacccga
     4081 cccgactcac ggccgaggac atcgagcgcc tgcaccccgg cctgctcagc gccctggccg
     4141 cccaccccgg catcgggttc gtcgtcgtgc tcacggcggg tgggccggtg gccctcgggg
     4201 cgaacggcac ccacgacctc acgacgggtg tcgtcgtcgg tgacgacccc ctggcgccct
     4261 tcgggccgga tgccgtgagc gacttcgtgc acgtgagcgg gttccccaac gctcccgaca
     4321 tctacctcaa ctcgctctac gaccccgtgc tcgacgaggt cgcggccttc gaggagctcg
     4381 tgggctgcca cggagggctc ggcgggtggc agacccgtcc gttgctggtc caccccgccg
     4441 ggtggaccgt cgacgaggac ctgctcgacg agcgcgggcg cctgcgcggg gctgacaccg
     4501 tgcaccacca gctggtgcgc tggctcgagc gactcggcca ccgcgagaac ctcgctgcct
     4561 gaggagggac ggcgacggca tccgcgcgtt ggctaccagt gacgccgatg acgccgtacg
     4621 cttggggtgc cgcgtcggtc gacgcgggcg acatgcagtg gcacggagat ctgggcatgg
     4681 tcccacgcac gcgttcggcg tgagtcccga tgccgggtgg tcagcgcgga gggtcttccc
     4741 cgtcggacgc actcgacacc gacgccaacc cgtcgtgcct gactgaggag aaggatcgct
     4801 tgtcacagcg agctccgcgc cgtggaggtg cgacccgcac cgcaccgcgc gaccatgcgc
     4861 gttccggcac ccccgacggc gagggcgtca tccccgtgct ggcccggacc gtccgccagg
     4921 tcgaggctgc ggcccagcgc ggcaaggtcc gccccagcca gcgcaccgcc taccaggtcg
     4981 tggcgctgct cgtgcgtgag gagcgcgccc gcgtcaaggc agacacgacc atcagcgagt
     5041 ccgagcgcac cgcgatgctg acccgtctcg agggcatcgc cacgatcctc gcgaagaccg
     5101 cggcgcgcga cacctcgctg ctcgagctgc tcaccgacgg cgccgtcgtg tcatccgccg
     5161 ccatcgacct gaagaacgac ctgctgcgtg cggccggccg cgaacccgag cccgaaccca
     5221 ccccagtggt ctcgccgacc gccccgttcg catcggcgtc gccgcgcgga gtcgtgccca
     5281 agaccgtcat cgcccggcag ctcgcgaatc cgttcctcgc gccggacttc tcggctctcc
     5341 ccgcgcccaa gccgcggacg aaccgactcg gcgggtggga gctgctctcg cccctgttcc
     5401 gcgccttcga gtacggcggc gacgccacgt gcatgccgct gccggagccg acgcgagtcg
     5461 tgcacggcat caagggtctc gagctgatgc accaccaggc ccaggtggtc gccgccgctg
     5521 ccgaaggaca ccgcaccttc ctgctggccg acgagcccgg tctcggcaag acggctcagg
     5581 cgctgttggc tgccgatgct gctggtgcct ttcccctgct cgtcgtcgtg cccaacgtcg
     5641 tcaaggcgaa ctgggcgcgc gaggcggagc tctggacgcc gcgacgtgcg gcgacggtca
     5701 tccacggtga cggtgacacc gtcgacggct tcgcggacat cgtcatcgtc aactacgaga
     5761 tcctcgaccg gcacgtcggc tggctcgggg cgcacggctt ccgcggcatg gtcgtcgacg
     5821 aggcccactt catcaagaac aagtcctcgc agcgttccca gcacgtgttg gagctctccg
     5881 agcgcatccg gtccaagcac gcgcgagccc tgttcatggc gctgaccggc accccgctga
     5941 tcaacgacat cgaggacttc aaggccatct ggcagttcct cgggtggatc gactccacga
     6001 agccgcgtca cgccctgatg gcagcgctcg aggagaccgg gctgaccccg gccgaccacg
     6061 ggttctacgc cgccgcccga acgagcgtca tcaaccaggg catcgtgcgt cgccgcaagg
     6121 tcgacgtcgc ctccgacatc ccggcacgac gggtcgcgga cctgcccgtc gagctcgacg
     6181 acgaggcgag ccgttccatc cgggccgccg agcaggaact cgcggcgcga ctggtcaagc
     6241 ggtacgacgc ggcgctggcc gctcgctcgg tctccgtcgt cgtcgagggc atcgaccacg
     6301 acatggtccg caaggtggcg cagtgggaac gcaagaacgc caccacggcg accaccggcg
     6361 acaacgtgtt caccgtcatg cgcaagatcg gacaggccaa ggccggcctc gccgcggact
     6421 acgccgcgca gctcgcgcgc aacgtcggca aggtggtctt cttcgccaag cacatcgacg
     6481 tgatggacat cgccgagcag accctggcca agcggggcat cggctacgcg tcgattcgtg
     6541 gcgaccagac accggccgtg cgcaaggcca acatcgaggc tttcctcacc gaccccgaga
     6601 tctccgtcgt ggtgtgctcg ctcacggctg cgggcgtcgg cctcaacctg caagtggcct
     6661 ccaacgtcgt gctcgccgag ctgtcgtgga cctcggccga acagacgcag gcgatcgacc
     6721 gggtgcaccg gatcgggcag accgagcccg tgacggcctg gcggatcatc gcggcgcaga
     6781 ccatcgaccc ccgcctggcc cagctcatcg acgacaaggc cggtttggcc gctcgggcgc
     6841 tagacgggtc ggatgaggaa gttggttcct ctgctgacct tcagctcgag gcgctcatgg
     6901 cgatgctcac ggacgcgctc gaggcccgcg ggttcccggc ctgatcaggg cctggggtgc
     6961 aggcgcagga aggctccgag cgcgatgagc tcgtcgggtg agcccggcga gaagtcggtg
     7021 agcacccgcg agcgcacgac gtaggccgcg tactgccccc gcgagatgcc cacgttgagc
     7081 cggtgtcggt cgagcaggaa gcccattccg cgcgatacct cggagtgcga cgacgccgcc
     7141 atcgacagga tcgccaccgg cgcctcctgc ccctggaact tgtcgacggt gcccacgcga
     7201 acgtcctcca gcccggcacc acgaagcact gctcggatgc ggtcagcctg gcggttgtac
     7261 ggcgtgatga cgatgaggtc cctcgctgtc agtggccggc caggcgctgg cgcacccgga
     7321 ccaccaggcg ctggcgcacg ggggccgtcc gtcgcggagg ggtcgtgcca catccgcccg
     7381 atgagcgtct gtgccagcga gcagaccgcc tcggcctcct ccagcgactc ggtggagttg
     7441 tcgaggtggt cgacgacgac gacgtgcagg ccggggcgga tgccgtccag gcgccgggcg
     7501 gcggtcacct gttcgtatga ggtcagcgcc ccgtcgtagg cgaggtcgga gaccacggtg
     7561 gtgaggtcgg ggtgcatccg gcgggtggtc tcgaggaagt agccgaggtg agcaggcagc
     7621 actggttcac ccgcggccag ccacccgagc gccgactcgt cgaccggttg ggcatgcacc
     7681 ccggtcgaca cctggggcag ttgctgcggg tctcccagca gcagcagccg ccgggcgcag
     7741 acccccacgg ccaaggtctt ggccacgctg aactgaccgg cctcgtcgat gacgagcagg
     7801 tcgagctggt cgcgagccac gcgacgctcg ttggtgaggt cccaggcgct tccgccgatg
     7861 acgtaaccac gccccacctg gtcatgttgc gcggcgaagg ccgcgaggtc gtcggccttg
     7921 ggcagggagg tccacgccgg gtcgggcgtc gccttggcga ctttgcccac ctgggcagga
     7981 tcgagcccgg cgcgcaccac cgctgacagc acgttctcga cggcggcatg gctctgggcg
     8041 cagaccccga tccgccatcc ccaggcggcg agtcgtgcga tgacgtgccc gccgacgtgg
     8101 gtcttgcccg tgccgggcgg gccctgggcc gcgacgtagg agtcgtccat ggcgagcagc
     8161 gctgcctcga tggcgttgat gacgcggtcg tcgcccgcgt cggccaccgg cggcaaggca
     8221 gcgccacccc tcaaccgcgg tgcccgacgc agcagcacgt cggtgcccgc cgacaccgga
     8281 accgcccccg acgccagcac ctcctcggcg acctcggcca gggcccggtc gatcgagtcg
     8341 gtcttgatgg ggccacccgg ggtgagggcc acggggaggt cgtcgtggtc acccaacgac
     8401 cgggtgtgct gctcgctgag ggtgacgacg aggcgcgcca ctccgtcggc ggacagctct
     8461 tcatcaatgg acagcacggt gcccttgctg gggttgaccg tgttggcatc catgggcggt
     8521 gcctcgaacc ctgcaggccc gggcgccgcg tagacgacgt ggcactcgcc cacgcgtggc
     8581 gctccgccag cggcctctcc ggtgaggcgc aggacccgac gggggttgtg ccgggcggac
     8641 tgctgcgccc acggctcgac gacctccgcg cggtcgacga cgaagacgcc gtcggcgcgg
     8701 cgccagtcac cgacgggcag gcgcagccgc tcgtagtgct cctgccacgc cggtttgtcc
     8761 tcacgggcat ggtagagaac cgacgaggcg atcatcgcca tcgcgcgctg ctcgtcgctc
     8821 cactgcgccg gggggatgtc gtcgaccagc gctcgaacgg ccgcttcgag agcaagagca
     8881 gccgcccgct tgtcgctgac cggtgctgcg gaggcaccgt cgtcgtcgac cgggcccgcc
     8941 ggggaaccac ccgcgtcccg gcaccgagcg agcagccagt cgcgcagcct cagggtggag
     9001 acgcagtcga gctcgttgta gtccgcgatc tcggccagca gtgacgcggc ttcgtcggtg
     9061 cggccctcgt tggtggcagc ggagaactgg tggtagacca cgatgctctc ggccccgccc
     9121 tgcacctcac cctcgcgtgc cggcatgtag agcggctcga gcttcttgat ggagtacgac
     9181 cgctgcgaga tgcgcacccc ggccttgacc accgcgtaga ggtcgacgaa gacgcggcgg
     9241 cgcaggaggt catcgacctc gtcctcgtag acaccgtgcc tggcggccat ccgcagcagg
     9301 gcagccggct catacggcgc gtagtggtag acgtgcaggt ctggccaggt cgccaggcgc
     9361 gcgttgacgt ggtcgacgaa ggagacgagg gcctgcttct cctcgcgccg gtcgtgggcc
     9421 cagaacgccc ggaaccgttc ctccccgctg tcgacctcga tcatcccaaa gaggtactcc
     9481 aacccccaga tgcctgaccc cggctcctgc cacagcgggt cgccctcgaa gtcgaagaag
     9541 atgtcgcccg tgctcggtgc gggcagtcgg ctcagggccg cgacgtcggt cacctcgtgg
     9601 tgcaccgcgc cggtggggtc ggcctcctgc acgagctgca gccgggcctg ggcccgcaac
     9661 cggtcgagcg actgcgccct gacgtcggcc accggcccgg tgctcgatgc caggtcgtcg
     9721 atggtgcgca ccccggcctc gtgcagccgt cgacgggtgg gccgtcgcat cccggcgacg
     9781 agcagcaggt ctcgtgctgc ctcgacctcg gccacgcacg tcgcgcagga cccgcaggcc
     9841 aaccagcgct catcccccca cgccgcgggt gcctcgtcgg cacggtggtc ggcgaggatg
     9901 ccgtcgagcc gggctcggcg gctgcggtag accgggagca cctcaccgag cgggtcgtca
     9961 cgcgtggccc cgtcgccgag caccaaccgc acgatcggcg cgaccggtgc cccggcatcc
    10021 agcaggaggg cggcgtaggc gccgacctgg agcagggcgg agacggtggc gtggcgggcc
    10081 agcttggtgt cactgaccag ccagccggcc ggggtgagct cgaggaagtc ggcgcggccg
    10141 acgaacccgc cgtcgaagac ggtggcctgg ctgaccacct cggctcccga gctcagcgtc
    10201 tcgagggtgg tcgtcatcgc ggcggccagg tcgtgcggtg tgttcgacgg tgtcggcagg
    10261 tggcggaccc ggcccgggtg ctcgcgactc agccgtcgca gctcggcctg ttcgtgctgg
    10321 tcgccaaggg ccttgacccg gtcgagcatc gggtcgcggg gctcctcgac ccgctcggcg
    10381 cggccgagca gcacgtcgag ctggcgcacg acggcgaact cgcacgcgga tgccgtgcgc
    10441 aggtcgctgg cgctgatgac gagccgggcg tcgtcgccga ggaggagcat gcaccgaacc
    10501 taacaacggc gaccgacacg gcctgcggac ggcatcccca ctgcggagtc gacgcgcagg
    10561 tgcacggttt cgcgggcggt ctcacctcga ccgcgacccc cctgacgtca gatccagctc
    10621 ggcagccaca tccgccagcg ccagtggtcg taggggatgg tctccccgac gtagatgggg
    10681 tggaagaacg cgaacatggc cagggtcagc aggaggtagc ccccgacgat gaaccagcgc
    10741 gtgcgccatc gctgcgggtc gtcctcgcgc cgcccgacga acagtgcgat gacgaacgtg
    10801 accgcgagca ccacccacgg ctcgaacgcc acggcgtaga aggcgtagat cgtgcgatcg
    10861 gcgaaggcga accacggcag gtagccgccg acgatgccgg ccgcgatcgc ccctgcccgc
    10921 cagtcccggc gcaccagcca ccaccacacg accaccgcga gggcgagcac gccgagccac
    10981 cacaccgtga tcgtgccgac ggggttgatg gcctgcgagc actggtcgac ggtgcacccg
    11041 gcgaccccgc gcgtcgggct ctcgtagtag aaggacgtcg gccgcagctg caggatccag
    11101 gtccaggggt cggactggta ggggtgcggg gtctgcagcg tcgtgttgaa ggcgaaggcg
    11161 tcctggtggt acttcaccca gttgcgcagc acagggggca gccacatgac gccctcgccg
    11221 ggatggaagg tgttccactg ccggtggtag ccgacgtcgc tgcggaacca cccgatccac
    11281 gacgccacgt agaccacggc ggtggtgccg accatggcca gcgccgcgaa ggggccgtcc
    11341 ttgagcaccg ccccgagacc ccagcggcgc accccggctg cgcgtcgagc gcccatgtcc
    11401 caccacacgg tcatcagccc gaacgccgcg aggaagaaca gccccgacca cttcgtgctt
    11461 gtcgcgaggc ccagcgacaa cccggcgacc cagcgccacg ggcgccagcc cagccacggc
    11521 cccatcccct gcggccacgt gccccgcggc agcgccccca cgcgcgcggc gaggatctcg
    11581 cggctgcggt cgcggtcgat gagcagcgcc gcgaacccgc acagtgcgaa gaacgacacg
    11641 aagatgtcga ggatgccggt gcgtgacatc acgaagtgcg acccctcgaa ggccagcagg
    11701 aaggccgcga cacagcccag cgccgtggag ccgaacatcc gccgggccac ccgcccgagg
    11761 atgaggatgg acagcgtccc gacgaccgcg gccgacaccc gccacccgaa ccccgaatcg
    11821 gacccgaaga cccactggcc ggccgccagc atccacttcc cgacgggagg gtgcacgacc
    11881 atgtcgccgt cgacggtgga gaagacgtcc gggttgccgc cggtgaagac cttgtccggc
    11941 tccttcaacg tgctgagcac ccgcatctcg acgccgaagc gcagcatcga ggccgcctgc
    12001 ttgacgtagt acgtctcgtc gaagaccatc gcccgcgggt ggtcgaggtt ccagaaccgc
    12061 aggaacccgc cgacgagggc gatcaggccc gggccgagcc acccccacag cttgtcggtc
    12121 atcagtgaca ccggccgtgg gccgagcagg cgcaggcgca gctcgtcggt ccggttcacc
    12181 gggtcatcct aggtcggggc ggccggatcg gccgacctgg gcagaccggg gctgaacgct
    12241 cggcaccgtg cgaggatcgc gcccatgagc gaccacagcg gcagcagcgc cgggagcggt
    12301 gcgagcagcg gcggcaccgg tggcagtggt gtcctcgtcc tggcagccac ccccatcggc
    12361 gatccgcgcg acgcggcgcc ccgtctggcc gaagagctgc gcacggccga cgtcgtcgcc
    12421 gccgaggaca cccgtcggtt gcgtcgcctc tgccaggccc tcgaggtcga gcccaccggc
    12481 cgcgtgctgt cctaccacga gcacaacgag gcgcagcgca cgcccgagct cgtcgagcag
    12541 ctgctcgcag gtcgccgcgt cgtcgtcgtg acggatgccg ggatgccgtc ggtctccgac
    12601 cccgggtacc gcctcgtgag cgccgcggtc gcgggcgggg tgcgtgtgac gtgcgttccg
    12661 gggccgtccg cggtgctcat ggcgttggcg gtgtccggga tgccggtcga ccggttctgc
    12721 ttcgagggtt tcctcccacg tcgcgcgggg gagcgccgga ccgcgctcgg ggtgctcggc
    12781 gacgagcggc gcaccatggt cttcttcgag gccccacacc gtctcgagcg caccctgacg
    12841 acgatggccg aggtgttcgg ggcgcagcgc cgtgccgcgg tgtgccgcga gctgaccaag
    12901 acctacgagg aggtcaggcg gggcacgctc gcggagctgg tcacctgggc tgccgacggg
    12961 gtgcgcggag agatcaccgt cgtcgtggag ggggcca
//
LOCUS       DAOSOC010000054        12986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4820_length_12986_cov_9.948032, whole genome shotgun sequence.
ACCESSION   DAOSOC010000054 DAOSOC010000000
VERSION     DAOSOC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4820_length_12986_cov_9.948032"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1390
                     /locus_tag="PL014_05920"
     CDS             59..1390
                     /locus_tag="PL014_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT56662.1"
                     /translation="MPSSAGTLPQDPAARVRDLEQVVAHQAALLEREDEVHRILVRVV
                     LAGGSLHDVCDAVAGFFGGAALVTTTDGRVLAIAGDESEVERVRALPCFDRTGRLLVE
                     SEPVGERDGSPTGADPTRALVKIAAGGSDHGLLGAFSPDAALTPTDVHMLERAATVAA
                     LAITKEQAVAAVEGKYRAEFLRDALAGRAGESGDAVSHAASLGWNLARPVVVVVAETD
                     ENDEQTTRSPEEVRGLQERFVRAWVQAVRARDASVPVAGFSQEVVALLPADPTASADA
                     VMRTVADIAKVVRGDGGGGRRSFSTGVSRPITSVGGLPAAYAEARKAVHVGRQMHGES
                     ALTHFDALGIYRLLALIPDGGDLRRFVDESLGELATDDSPENEDLRRTLSVLIDTNIN
                     VAETARQLFFHYNTLRYRIAKLERMLGPFTTDAQLRLTLALALRIHQMRGI"
     gene            1541..3016
                     /locus_tag="PL014_05925"
     CDS             1541..3016
                     /locus_tag="PL014_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOT56663.1"
                     /translation="MALGLGWKVKDAAEVAAGGVVAPDERLSWGKTVGLGAQHVVAMF
                     GATFVFPLVMGLNAQLAIMMSGIATILFLLIVQGKVPSYLGTSAAFVGAVAAIRAQEG
                     ATSADVTGAILVAGLVLAAVGLLIHVAGPSILHKVLPPAVTGAVVMLIGFNLAPVVAN
                     IYWPQDQWVALATMLFTVVVAVAFRGFIGRIAIFLALIFGTALSWLLDTVSGPITSVL
                     GGATEATEHLRWNTAGLGDAAWFGLPAKTMMGADGKEIVGWHFPSFSMTAIILVIPAV
                     IALVAENTGHVKAVAEMTGHDLDPVMGRAIAGDGIGTAVAATFGGSPTTTYAENIGVM
                     AATRVYSTAAYYVAAAVAILFGLSPKFGALVASIPGGVLGGITVVLYGMIGLLGAKIW
                     KENGVDFANPINLVPLAAGIVIGIGDVKMKVSDDFTLSGIALGTIVAIAGYHLARVIA
                     PSELREHADGTALAVGDYVYGDADQIDDLYQDGYPGSTDRK"
     gene            3108..4010
                     /locus_tag="PL014_05930"
     CDS             3108..4010
                     /locus_tag="PL014_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOT56664.1"
                     /translation="MKPGPFVHHAPTTVDEAVAVLGEVGHDGKVLAGGQSLIPVLSMR
                     LATPGHLVDINGVAGLDAVHVTDTAVRIGALVRHAGLEQHDGAATALPLLRQALRNVA
                     HPAIRNRGTTVGSIAHADPAGEMPSVLVLTDGVVEAVGPGGRREIAATDFFLGPLETS
                     LADDELVEAVRFTRFGPGTRTAFLESSRRHGDYALAGVAVAVDVADGVVVSARAGFVS
                     VTPVPLVLDLTPVLRGRDAGAPVPDEVTDAVREFVDPEGDIHATADYRRMLAAELTRR
                     ALATATHPTPAADPAPTTAQESVA"
     gene            4007..4585
                     /locus_tag="PL014_05935"
     CDS             4007..4585
                     /locus_tag="PL014_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOT56665.1"
                     /translation="MSEQTTDTPPEQLLSISLRVNGVTRTASAPARRLLSDFLRHDLG
                     LTGTHVGCEHGVCGACTVLVDGQPMRACLMFAVSAQRHEVTTIEGIGNDPEKLSPVQQ
                     AFTECHALQCGFCTPGFVTTITAYLEDNPDPTPEEAREAISGNLCRCTGYQNIVSAVC
                     RAAEIRRDRADRTEIDQSVDLGTTDGEGEGSA"
     gene            4582..6993
                     /gene="cutA"
                     /locus_tag="PL014_05940"
     CDS             4582..6993
                     /gene="cutA"
                     /locus_tag="PL014_05940"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HOT56666.1"
                     /translation="MSTRQFGERVQRREDPRLLTGNGRYLDDLGLGALTAAFVRSPHA
                     HARIVDIDIDDAWEVDGVEAIYTWEDLTGRVAEPLPVLIPHPALTHPRTGYCLAKDEV
                     NHVGEAIVMVIARDRYLAEDAAQRIRVTYDSLPVVVGVEAARAAQTLVHDDAPGNIAA
                     HLLQEVGDVTAAMAAAPNTLTLDLEIERSACMPMEGKGVFARWDADEQSLRIYSSTQT
                     STGVRAAVAAKLGMPLAKVECIAPDVGGGFGVKIMHPWPEEVLVPWAARLLGRDVKWT
                     EDRREHFISSAHERGQLQQVTVGFDDEGHLLALDVQFWHDNGAYTPYGIIVPIITATQ
                     LLGPYKPGAYRVEFWSLFTNTVLVTPYRGAGRPQGCYAMERTMDAIAAHLGLDRAEVR
                     SRNFIQPEEMPYDHGLMFQDGRPLKYDSGDFPASLAKLKALVGWDDFAAYREEAAAEG
                     RRVGLGIGCYVEGTGVGPYEGGHVHVETSGRVNVATGLTTQGQGHQTVLAQIVADELG
                     VRIEDVHVTTGDTRRMAYAVGTFASRGAVMSGNAVALAAKAVRAKALRIAADALEVSA
                     DDLQIEDGVISVKGAPGTSIALSTVAVLSNPLRYAFDESSKAATQFAGTFDPDKPPVA
                     DDEEPGLEGQDFYSPTQATFANGMHAAIVETDPVTAEIRILRYCVIHDCGVMINPMIV
                     EGQVHGGVAQGVGGALYERMAYDESGQLLNASFMDFLMPYASEVPHVETDHLETPSPL
                     NPLGLKGAGEAGTIPVTAVIASAIEHAEGFPVRSMPISPSELWELRRKHAESPVPVPA
                     QEGSR"
     gene            6990..7667
                     /locus_tag="PL014_05945"
     CDS             6990..7667
                     /locus_tag="PL014_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HOT56667.1"
                     /translation="MKISGGSTLDAPVEQVWEAIQDPAVLARCIPGCEALRTVGEDRY
                     AMSVTAGVAAIKGTYAGEVSLHDKVAPNSLRLKATGAGAPGTIDATVMVHFAPSAGGG
                     TDLTYDADASVGGAIGGVGQRMLAGVTKKMAGQFFTALDQDIAGVLVPAEATTALGAP
                     VAGGAPTASGVPAAAEGVFPGRQATANANASISMDSATTFGAGALVGGLLVAIGVLIG
                     SRLGRRH"
     gene            7667..>8514
                     /locus_tag="PL014_05950"
     CDS             7667..>8514
                     /locus_tag="PL014_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT56668.1"
                     /translation="MRPFTAAAVQLAPAPGPLTPESVAANLEAMVDITRRCHAATGAE
                     LIVLPESATTGFTPACSIEQLWELMSELPGPVIDPLRAVAAELGVHLVAGTYERGPTP
                     DIVYNSSVLIDPAGELLGVYRKTHPFCSEAVSGGGWVTPGDTVTVCDTDLGRIGMIIC
                     FDGDYPELSRIQAVQGAEVICRPSALLRSADIWELTSRARAYDNHVYVIGANAIGADP
                     AGVLYFGNSHIVTPVGHIVAKAATHKGWVSARLDPTEALASLTPGSNIGQGFDHLRDR
                     NLDLIRR"
     assembly_gap    8515..8614
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8804..9322
                     /locus_tag="PL014_05955"
     CDS             8804..9322
                     /locus_tag="PL014_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56669.1"
                     /translation="MLTALLVIGGIGILMLLISFVAGEALDGMLESVGPDFLSGLAVA
                     GFLAAFGFVGALVLDLTDSNGAALGAGLVAGALVGVGAGKASQVLMRGGDESTVRSSG
                     LVGLTGSVIDAIPADGLGQVSVVAAGHITRLNARADEPLPNGTAVTVTAVLSPTSVRV
                     ARTGGSSRPADA"
     gene            9439..11010
                     /locus_tag="PL014_05960"
     CDS             9439..11010
                     /locus_tag="PL014_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT56670.1"
                     /translation="MFGLEIPVALISVLGLVTLLFLLGLIIASRYKVAGPNEAFIVTG
                     RPGKEVRNPETGIMSTDLSGQKVVMGGGVFVVPFVQRLHVLDLSSRRIMIQIRSAVSG
                     QGVKLNLDGVAIVKVGGNEDSIRAAAQRFLTQQGEIETFTQETLAGSLRSIVGSLSVE
                     QIIRDRAAFAQRVADEAESSLTGQGLILDTFQIQDITDDGSYLSDLGRPESARVGQTA
                     AIAEANARQAAEQARLAAEQEIAVANRQLALKQSEIKAETDAAAATAAAAGPLAQADR
                     EQVILTEQEKVAVRQAALKERQLDTEVRKPADAERYKVEQEAEGARNAAIAEADARRQ
                     ASIAAAQAEAEKARLTGEAEKSRRTALADAEAIEGERRGQADKALRVAQAEALRAEGE
                     AQASAILATGQAEAEAMNKRADAFARYNEAAVLQMLIEVLPKVAHELAAPMGSIDQLT
                     VLSTEGAGALPKQVTDNLTQTMAMLKNTTGIDIQSIVQGFGDGSSTKTTTTTERPVKG
                     SAVSAPSATAASTDE"
     gene            11105..>12986
                     /locus_tag="PL014_05965"
     CDS             11105..>12986
                     /locus_tag="PL014_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOT56671.1"
                     /translation="MATAQTSTSNRRIRVGIDTGGTFTDVVAFDEDTGELVTTKTPST
                     PANPADGFIAGIEKILGVLGATGTDITAVSHGTTVATNKLLEGKVERLGFITTEGYEF
                     MLEIARQAVPDGYGNSYFWVKPPRIVEADHVRTIGGRMDFEGNELRPFDEADAVAAAR
                     WFRERGITTIGVCLLHSYANDTHELAMREVLRREHPEAVVSISSQVLREYREYERSMT
                     TLVDAAVKPNVSRYVRNIHERLDGFTGGRPIPFYVMKSNGGVLSADEVVHQPITTVLS
                     GPAAGALGAALIARRAGFDRVLTCDGGGTSTDVSVVLDGEPTLTTEGTVGAYPSKIPM
                     IDVVTVGAGGGSIAWVTPEGTLKVGPQSAGADPGPLCYGKGGTEPTITDAHVVLGRIP
                     PHLLGGEIPLDVDAARAGIRDLAARLGLDEERCATGILEISAWNQANALRQVSVKRGL
                     DVRDFMLTTFGGSGSLLACRLVDILDLAGVVVPQNPGNVSAFGLLTVDVKNDYVQTHV
                     ARHAGLDRSAVEKVLDELTALATAALDTEGFPREQHEHVRTADLRYFGQAYEVRVPIG
                     AGPLDEAAAEAVAQRFHDEHRQLYGYDFRDDPRQQVEWVNLRVTGIGPITRPELREIA
                     PRD"
BASE COUNT         1904 a         4800 c         4391 g         1791 t
ORIGIN      
        1 ggtctacagt gtccggatgg tcattgttga caacgatcgg ccgcgagacg cacagacctt
       61 gccatcgtcc gccggcaccc tcccgcagga cccggccgcg cgcgtccggg acctcgagca
      121 ggtcgtcgcc caccaggccg ccctcctcga gcgcgaggac gaggtgcacc gcatcctggt
      181 ccgggtggtc ctcgccggcg ggtctctcca cgacgtgtgt gacgccgtcg ccgggttctt
      241 cggtggcgcg gcgctcgtga ccaccaccga tggccgcgtg ctcgccatcg ccggcgacga
      301 gagcgaggtc gagcgggtgc gtgccctgcc ctgcttcgac cgcaccgggc gactgctcgt
      361 cgagtccgag cccgtcggcg agcgcgacgg cagcccgacc ggggccgacc ccacccgcgc
      421 cctggtcaag atcgccgcgg gcggttccga ccacgggctg ctcggcgcct tctcccccga
      481 tgccgccctc accccgaccg acgtgcacat gctcgaacgt gccgcgaccg tcgccgccct
      541 ggccatcacc aaggagcagg cggtcgccgc cgtcgagggc aagtaccgcg ccgagttcct
      601 gcgcgacgcc ctcgccggtc gcgccgggga gtcgggggat gccgtgtcgc acgccgcgtc
      661 cctgggctgg aacctcgcgc ggccggtcgt cgtcgtcgtg gccgagaccg acgagaacga
      721 cgaacagacc acccgctccc ccgaggaggt ccggggcctg caggaacgct tcgtgcgggc
      781 gtgggtgcag gccgtgcgcg cgcgcgacgc gagcgtgccc gtcgcgggct tcagccagga
      841 ggtcgtcgcc ctgctcccgg ccgaccccac ggcatccgcg gatgccgtga tgcggaccgt
      901 cgcggacatc gccaaggtgg tgcgcggcga cggcggtggc ggccggcgca gcttctcgac
      961 cggggtgtcg cgaccgatca cgtcggtggg tggcctgccg gccgcctacg ccgaggcccg
     1021 caaggcggtg cacgtcgggc gacagatgca cggggagtcg gccctgaccc acttcgacgc
     1081 gttgggcatc taccgcctgc tcgccctcat ccccgacggg ggcgacctgc gccggttcgt
     1141 cgacgagtcc ctcggcgagc tcgccactga cgactccccg gagaacgagg acctgcgccg
     1201 caccctgagc gtgctcatcg acaccaacat caacgtcgcc gagaccgccc ggcagctgtt
     1261 cttccactac aacacgctgc gctaccggat cgccaagctc gagcggatgc tcggcccgtt
     1321 caccaccgac gcgcagcttc ggctcaccct cgccctggcc ctgcggatcc accagatgcg
     1381 cggcatctga gggcacctgc gcacgattgc catgcatcac ggcatccggc tgtcaatgat
     1441 tgccgagcat gtcgcgcacg gggggtcact accttgcctg tatcactgct aggtgagccg
     1501 cgtcactggt gccgcggtca gacaagggag gtccccggaa atggcactcg gtctcggttg
     1561 gaaggtcaag gacgccgctg aggtggcggc cgggggcgtc gtcgcccccg acgaacgcct
     1621 ctcgtggggc aagacggtcg gtctgggcgc gcagcacgtc gtcgccatgt tcggggcgac
     1681 gttcgtcttc ccactcgtca tgggcctgaa cgcccagctc gcgatcatga tgagcggcat
     1741 cgcgacgatc ctgttcctcc tcatcgtgca gggcaaggtc cccagctacc tcggcacctc
     1801 ggcggccttc gtcggtgccg tggccgcgat ccgggcccag gagggcgcga cgagcgcgga
     1861 cgtcaccggc gccatcctcg tcgccggcct cgtgctggcg gccgtgggcc tgctcatcca
     1921 cgtcgccggc ccgtcgatcc tgcacaaggt gttgccgccg gcggtcaccg gtgcggtcgt
     1981 catgctcatc ggcttcaacc tcgccccggt cgtcgcgaac atctactggc cccaggacca
     2041 gtgggtcgcc ctcgcgacga tgctcttcac ggtcgtcgtc gccgtcgcgt tccgcggctt
     2101 catcgggagg atcgcgatct tcctcgcgct gatcttcggc acggcgctgt cctggctgct
     2161 cgacaccgtg tccggcccga tcacctccgt gctcggcgga gccaccgagg ccaccgagca
     2221 cctgcggtgg aacaccgccg ggctcggtga cgccgcctgg ttcgggctgc ccgccaagac
     2281 gatgatgggc gccgacggca aggagatcgt cgggtggcac ttcccgagct tctcgatgac
     2341 ggccatcatc ctcgtcatcc ccgcggtcat cgccctggtc gccgagaaca ccggccacgt
     2401 caaggccgtc gccgagatga ccggccacga cctcgacccc gtcatgggtc gcgccatcgc
     2461 cggtgacggc atcggcaccg ccgtcgccgc gaccttcggc ggctccccga cgacgaccta
     2521 cgccgagaac atcggtgtca tggcggcgac ccgggtctac tccaccgccg cctactacgt
     2581 ggccgcggcg gtcgcgatcc tgttcggtct ctccccgaag ttcggcgccc tcgtggcctc
     2641 catccccggt ggcgtcctcg gcggcatcac ggtcgtgctc tacggcatga tcggcctgct
     2701 cggcgccaag atctggaagg agaacggggt cgacttcgcg aacccgatca acctcgtgcc
     2761 gctcgctgcg ggcatcgtca tcggcatcgg cgacgtcaag atgaaggtca gcgacgactt
     2821 caccctgagc ggcatcgccc tcggcaccat cgtggccatc gccggctacc acctggcccg
     2881 cgtcatcgcg ccgtccgagc tgcgcgagca cgccgatggc acggcgctgg ccgtcggcga
     2941 ctacgtctac ggcgacgcgg accagatcga cgacctctac caggacggct acccgggttc
     3001 caccgaccgc aagtaacccc cacgaccacc ccgcccgggt cgacccctcg agcacccggg
     3061 cggggtgcct ctttcccgac caacccccga caggcaggat cgaccaggtg aaacccggac
     3121 cgttcgtgca ccacgccccc acgaccgtcg acgaggccgt cgccgtgctc ggcgaggtcg
     3181 gccacgacgg caaggtcctc gccggcggcc agagcctgat cccggtgctg tcgatgcgcc
     3241 tggccacccc gggccacctc gtcgacatca acggcgtggc ggggttggat gccgtgcacg
     3301 tcaccgacac cgcggtgcgc atcggtgcgc tcgtccggca cgccgggctc gagcagcacg
     3361 acggggcagc caccgccctg cccctgctgc gccaggccct gcgcaacgtc gcccacccgg
     3421 cgatccgcaa ccgcggcacc accgtcggct cgatcgccca cgccgacccg gccggtgaga
     3481 tgccctccgt gctcgtgctg accgacggtg tcgtcgaggc ggtcgggccg ggtggccgcc
     3541 gcgagatcgc tgcgaccgac ttcttcctcg gcccgctcga gacgagcctg gccgacgacg
     3601 agctcgtcga ggcggtgcgg ttcacccgct tcggccccgg cacccgcacg gccttcctcg
     3661 agtccagccg ccgccacggc gactacgccc tggccggggt ggccgtggcc gtcgacgtcg
     3721 cggacggtgt cgtggtcagc gcccgcgccg gcttcgtgtc cgtgacgccc gtgccgttgg
     3781 tgctcgacct gaccccggtg ctccgcggtc gggatgccgg tgctccggtg cccgacgagg
     3841 tcaccgatgc cgtgcgcgag ttcgtcgacc ccgagggcga catccacgcc accgccgact
     3901 accgccggat gctcgccgcc gagctcaccc gccgcgccct ggcgacggcc acccacccga
     3961 cacccgcagc cgacccggca cccaccaccg cccaggagtc cgtcgcatga gcgagcagac
     4021 gaccgacacg ccccccgagc agctgctgag catcagcctg cgcgtcaacg gggtgacgcg
     4081 cacggcatcc gcccccgccc gccgcctgtt gtcggacttc ctgcgccacg acctcggcct
     4141 caccggcacc cacgtcggct gcgagcacgg cgtgtgcggg gcgtgcacgg tgctcgtcga
     4201 cgggcagccg atgcgcgcgt gcctgatgtt cgccgtcagc gcgcaacgcc acgaggtgac
     4261 gacgatcgag ggcatcggca acgaccccga gaagctcagc ccggtccagc aggccttcac
     4321 cgagtgccac gccctgcagt gcgggttctg cacccccggg ttcgtcacga cgatcacggc
     4381 ctacctcgag gacaaccccg accccacccc cgaggaggcc cgcgaggcga tctcgggcaa
     4441 cctgtgccgc tgcacggggt accagaacat cgtgtcggcg gtctgccgcg ccgccgagat
     4501 ccgtcgggac cgcgcagacc gtaccgagat cgaccaatcg gtcgatctcg gtacgaccga
     4561 cggcgagggc gagggcagcg catgagcacg cgtcagttcg gcgagcgcgt ccagcgccgg
     4621 gaggaccccc gtctgctcac cggcaacggc cgctacctcg acgacctcgg gctcggggca
     4681 ctgaccgccg cgttcgtgcg cagcccgcac gcccacgcgc ggatcgtcga catcgacatc
     4741 gacgacgcgt gggaggtcga cggcgtcgag gccatctaca cgtgggagga cctgaccggc
     4801 cgggtcgccg agccgttgcc ggtgctcatc ccgcacccgg ccctgactca cccgcgcacc
     4861 ggttactgcc tggccaagga cgaggtcaac cacgtcggcg aggcgatcgt catggtcatc
     4921 gcccgcgacc gctacctcgc cgaggacgct gcccagcgga tccgggtgac ctacgacagc
     4981 ctgcccgtcg tcgtcggcgt cgaggccgct cgcgccgcgc agaccctcgt gcacgacgac
     5041 gccccgggca acatcgccgc ccacctgctc caggaggtcg gcgacgtcac ggccgcgatg
     5101 gccgccgcgc cgaacaccct caccctcgac ctcgagatcg agcgcagtgc gtgcatgccc
     5161 atggagggca agggcgtctt cgcccgctgg gacgccgacg agcagtcact gcgcatctac
     5221 tcctcgacgc agacctccac gggggtgcgg gcggctgtcg ccgccaagct ggggatgccg
     5281 ctcgcgaagg tcgagtgcat tgcccccgac gtgggcggcg ggttcggcgt gaagatcatg
     5341 cacccgtggc ccgaggaggt gctcgtcccg tgggcggccc ggttgctcgg ccgcgacgtg
     5401 aagtggaccg aggaccggcg cgagcacttc atctccagcg cgcacgagcg cgggcagctc
     5461 cagcaggtca ccgtcgggtt cgacgacgag ggccacctgc tcgccctcga cgtgcagttc
     5521 tggcacgaca acggcgccta caccccgtac ggcatcatcg tcccgatcat cacggccacc
     5581 cagctgctcg gcccgtacaa gcccggcgcc taccgggtgg agttctggtc gctgttcacc
     5641 aacaccgtcc tcgtgacgcc gtatcgcggc gccgggcgcc cgcaggggtg ctacgcgatg
     5701 gaacgcacga tggatgccat cgcagcgcac ctgggcctcg accgggccga ggtgcggtct
     5761 cgcaacttca tccagcccga ggagatgccc tacgaccacg gcctgatgtt ccaggacggg
     5821 cgaccgctga agtacgactc aggtgacttc ccggcatccc tggccaagct caaggccctc
     5881 gtcggctggg acgacttcgc ggcctaccgc gaggaggccg ccgccgaggg gcgccgggtc
     5941 gggctcggga tcggctgcta cgtcgagggc accggggtcg gcccctacga gggcgggcac
     6001 gtgcacgtcg agacgagcgg ccgggtgaac gtggccaccg gcctgaccac ccagggccag
     6061 ggccaccaga cggtgctcgc gcagatcgtc gccgacgagc tcggcgtgcg gatcgaggac
     6121 gtccacgtca ccaccggtga cacccggcgg atggcgtacg cggtcggtac cttcgcctcg
     6181 cgcggggcgg tgatgtccgg caatgccgtg gcgctggccg cgaaggccgt gcgggccaag
     6241 gcgctgcgga tcgccgccga cgccctcgag gtgtcggcgg acgacctgca gatcgaggac
     6301 ggggtcatct cggtcaaggg cgcacccggc acctcgatcg ccctgtcgac cgtggccgtg
     6361 ctctccaacc cgctgcggta cgcgttcgac gagtcgtcca aggccgcgac ccagttcgcc
     6421 ggcaccttcg accccgacaa gccgccggtc gccgacgacg aggaaccggg gctcgagggg
     6481 caggacttct actccccgac ccaggccacc ttcgccaacg gcatgcacgc ggcgatcgtc
     6541 gagaccgacc cggtgaccgc cgagatccgc atcctgcggt actgcgtcat ccacgactgc
     6601 ggggtcatga tcaacccgat gatcgtcgag gggcaggtgc acggcggggt ggcccagggc
     6661 gtcggcggcg ccctctacga gcggatggcc tacgacgagt ccgggcagct gctcaacgcc
     6721 tcgttcatgg acttcctcat gccctacgcc tccgaggtcc cgcacgtcga gaccgaccac
     6781 ctcgagaccc ccagcccgct caacccgctc ggcctcaagg gcgccggtga ggcgggcacg
     6841 atccccgtca cggcggtcat cgcctcggcg atcgagcatg ccgaggggtt ccccgtgcgg
     6901 tccatgccga tcagcccgtc cgagttgtgg gagctgcggc gcaagcacgc cgagtccccc
     6961 gttcccgttc ctgcgcagga aggttcacga tgaagatctc cggtggttcg acactcgacg
     7021 cacccgtcga gcaggtgtgg gaggccatcc aggacccagc cgtgctcgcc cgctgcatcc
     7081 ccgggtgcga ggcgctgcgc accgtcggtg aggaccgcta cgcgatgtcg gtcaccgccg
     7141 gggtcgccgc gatcaagggc acctacgccg gggaggtgtc cctgcacgac aaggtcgccc
     7201 cgaactcgct gcggctgaag gccaccggcg ccggcgcgcc cggcaccatc gacgcgacgg
     7261 tcatggtgca cttcgcgccg tcggccggtg gcggcaccga cctcacgtac gacgcagacg
     7321 cctcggtggg cggcgcgatc ggtggcgtcg ggcagcggat gctggccggg gtcacgaaga
     7381 agatggccgg gcagttcttc accgctctcg accaggacat cgccggggtg ctcgtgcccg
     7441 cggaggcgac gaccgcgctc ggtgcgccgg tcgcgggagg tgcgccgacg gcatccggtg
     7501 tgcccgcggc agccgagggg gtcttcccgg gacggcaggc gacggcgaac gcgaacgcct
     7561 cgatctcgat ggacagcgcc acgaccttcg gcgcgggcgc gctcgtcggc gggctgctcg
     7621 tcgcgatcgg cgtactcatc ggcagcaggc tcgggcggcg gcactgatgc gccccttcac
     7681 cgcggcagcc gtccagctcg ccccggcgcc ggggccgctg acaccggagt cggtggcggc
     7741 caacctcgag gcgatggtcg acatcacccg gcgctgtcac gccgccacgg gtgccgagct
     7801 catcgtgctg ccggagtcgg cgacgacggg tttcacgccc gcgtgctcga tcgaacagct
     7861 ctgggagctc atgtcggagc tgcccgggcc ggtcatcgac ccgctgcgcg cggtggccgc
     7921 cgagctcggc gtgcacctcg tggccggcac gtacgagcgt ggccccactc ctgacatcgt
     7981 ctacaactcc tcggtgctga tcgacccggc cggcgagctg ctcggggtgt accgcaagac
     8041 gcacccgttc tgctcggagg cggtgtccgg cggtggctgg gtcacgccgg gtgacacggt
     8101 caccgtgtgc gacaccgacc tcggccggat cggcatgatc atctgcttcg acggcgacta
     8161 ccccgagctg tcgaggatcc aggcggtgca gggggccgag gtcatctgcc ggccgtcggc
     8221 cctgctgcgc tcggccgaca tctgggagct cacctcgcgg gcgcgggcct acgacaacca
     8281 cgtctacgtc atcggcgcca acgccatcgg ggccgacccg gcgggggtgc tgtacttcgg
     8341 caactcgcac atcgtcacgc ccgtcggcca catcgtcgcg aaggcggcga cccacaaggg
     8401 ctgggtgtcg gcgcgtctcg accccaccga ggcgttggcc tcgctgaccc cggggtcgaa
     8461 catcggccag ggcttcgacc acctgcgcga ccgcaacctc gacctcatcc ggcgnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacatcg gccagggctt cgaccacctg
     8641 cgcgaccgca acctcgacct catccggcgc caccgggccg agctggaggg gcccgccgag
     8701 acctccttcc cgcacgcctg agagcgggta ggctcgctgg acgaggcggc cacgcgggcc
     8761 gccgccgacg accggcagcc actggccggc cacggagggg accatgctga ccgccctgct
     8821 cgtcatcgga ggcatcggca tcctgatgct gctcatctcg ttcgtcgcgg gcgaggccct
     8881 cgacgggatg ctggagagcg tcggccccga cttcctctcc ggcctggcgg tggcgggctt
     8941 cctcgcggcg ttcggcttcg tcggtgccct ggtgctcgac ctcaccgact ccaacggtgc
     9001 ggcgctcggc gccgggctcg tcgcgggcgc cctggtcggg gtgggcgccg gcaaggccag
     9061 ccaggtcctg atgcgcggcg gcgacgagtc gaccgtgcgc tcgtccggac tggtcgggct
     9121 caccggatcc gtgatcgacg ccatcccggc tgacggtctg ggccaggtca gcgtcgtcgc
     9181 ggccggacac atcacccggc tcaacgcccg cgccgacgaa ccgctgccca acggcaccgc
     9241 cgtcaccgtg accgcggtgc tgagcccgac ctcggtgcgg gtggcccgca cgggtggctc
     9301 gtcccgcccg gctgacgcgt agggcaggat cgggcagcga cgcgggggga accgggtagg
     9361 ggccgtcgac gtcgactgag cacacgacca agagcagtgg agcgccgcgc cgacgcgacg
     9421 cacgaaaggg cagacaacat gttcggactc gagattcccg tcgcactcat ctcggtactg
     9481 gggctggtga ccctgctctt cctcctgggg ctgatcatcg cctcgaggta caaggtcgca
     9541 gggcccaacg aggcgttcat cgtcaccggc cgcccgggca aggaggtccg caaccccgag
     9601 accggcatca tgtcgaccga cctgtcgggc cagaaggtcg tcatgggtgg tggcgtgttc
     9661 gtcgtcccgt tcgtgcagcg cctgcacgtg ctcgacctgt ccagccgtcg catcatgatc
     9721 cagatccgca gcgccgtgtc cggccagggc gtcaagctca acctcgacgg cgtcgcgatc
     9781 gtcaaggtcg gcggcaacga ggactcgatc cgcgccgcgg cccagcgctt cctcacccag
     9841 cagggcgaga tcgagacctt cacccaggag accctcgccg gttcgctgcg atccatcgtc
     9901 ggctcgctga gcgtcgagca gatcatccgt gaccgggccg cgttcgcgca gcgggtcgcc
     9961 gacgaggccg agtcctcgct caccggtcag ggcctgatcc tcgacacctt ccagatccag
    10021 gacatcaccg acgacggctc ctacctgtcc gacctcggcc gccccgagtc cgcccgggtg
    10081 ggccagaccg cggcgatcgc cgaggccaac gcccgccagg ccgccgagca ggcccgcctc
    10141 gccgccgagc aggagatcgc cgtcgcgaac cgccagctgg cgctcaagca gtccgagatc
    10201 aaggccgaga cggatgccgc ggccgccacc gctgccgccg ccgggccgct ggcccaggcc
    10261 gaccgcgagc aggtcatcct caccgagcag gagaaggtgg ccgttcgtca ggcggccctg
    10321 aaggagcgcc agctcgacac cgaggtgcgc aagcccgccg acgccgagcg ctacaaggtc
    10381 gagcaggagg ccgagggtgc ccgcaacgcg gccatcgccg aagcggacgc ccgccgtcag
    10441 gcctccatcg ccgccgcaca ggccgaggcc gagaaggcgc gcctcaccgg tgaggccgag
    10501 aagtcacgac gcaccgcgct ggccgatgcc gaggccatcg agggtgagcg tcgcggacag
    10561 gccgacaagg cgctgcgtgt cgcccaggcc gaggccctgc gcgccgaggg tgaggcccag
    10621 gccagcgcca tcctcgccac cggccaggcc gaggccgagg cgatgaacaa gcgtgccgac
    10681 gccttcgccc ggtacaacga ggcggccgtg ctccagatgc tcatcgaggt gctgccgaag
    10741 gtggcccacg aactcgctgc accgatgggt tccatcgacc agctcaccgt gctgtcgacc
    10801 gagggtgcgg gtgccctgcc caagcaggtg accgacaacc tcacccagac gatggcgatg
    10861 ctgaagaaca ccaccggcat cgacatccag agcatcgtgc agggcttcgg tgatggctcg
    10921 tcgaccaaga ccacgaccac gacggaacgc cccgtcaagg gttcggccgt gtcggcgccg
    10981 tcagccactg ccgcctcgac cgacgagtag gcccctgcag cgggctagtt ctgaggtgat
    11041 atccccggct cgttggcaga cattgccaac gagccggggc tgtatccgag attgaatctg
    11101 acgcatggcg acggcgcaga cgagcacttc gaaccggcgg atccgggtgg gcatcgacac
    11161 cggagggacg ttcaccgacg tcgtcgcctt cgacgaggac accggcgagc tcgtgacgac
    11221 gaagaccccg agcacgccag caaacccggc ggacggcttc atcgccggca tcgagaagat
    11281 cctcggggtg ctcggcgcga cgggcacgga catcaccgcc gtcagccacg gcacgacggt
    11341 ggcgacgaac aagctcctcg agggcaaggt ggagcgcctc gggttcatca ccaccgaggg
    11401 ctacgagttc atgctcgaga tcgcccgcca ggccgtgccc gacggctacg gcaactccta
    11461 cttctgggtc aagcccccgc gcatcgtcga ggccgaccac gtgcgcacca tcggcggccg
    11521 gatggacttc gagggcaacg agctgcgccc gttcgacgag gcggatgccg tggcggccgc
    11581 ccgctggttc cgtgagcgcg gcatcacgac gatcggggtg tgcctcctgc actcctacgc
    11641 caacgacacg cacgaactgg cgatgcgcga ggtgctgcgc cgcgagcacc cggaggccgt
    11701 ggtgtcgatc agctcgcagg tgctgcgcga gtaccgcgag tacgagcgct cgatgaccac
    11761 cctggtcgac gcggcggtca agccgaacgt cagccggtac gtgcggaaca tccacgagcg
    11821 gctcgacggc ttcaccggtg gtcggccgat cccgttctac gtcatgaagt ccaacggtgg
    11881 ggtgctgtcg gccgacgagg tcgtgcacca gccgatcacc accgtgctct ccggcccggc
    11941 cgcgggggcc ctgggggcgg cgctcatcgc ccgtcgcgcg gggttcgaca gggtgctcac
    12001 ctgcgacggc ggtggcacct ccaccgacgt ctcggtcgtg ctcgacggtg agccgacgct
    12061 caccaccgag gggacggtcg gcgcctaccc gagcaagatc ccgatgatcg acgtcgtcac
    12121 cgtcggcgcg ggcggtggct cgatcgcctg ggtcactccc gagggcaccc tcaaggtggg
    12181 gcctcagtcg gccggggccg accccggacc gctctgctac ggcaagggcg gcacggagcc
    12241 gacgatcacc gacgcccacg tcgtgctcgg gcgcatccct cctcacctgc tcggtggcga
    12301 gatcccgctc gacgtggatg ccgcgcgcgc cggcatccgt gacctcgccg cccgcctcgg
    12361 gctcgacgag gaacggtgcg ccaccggcat cctcgagatc tccgcgtgga accaggcgaa
    12421 cgcccttcgt caggtgtcgg tgaagcgcgg gctcgacgtg cgcgacttca tgctgaccac
    12481 cttcggcggg tcggggtcgc tgctcgcgtg ccggctcgtc gacatcctcg acctcgccgg
    12541 ggtcgtcgtg ccgcagaacc ccgggaacgt gtcggcgttc gggctgctca ccgtcgacgt
    12601 caagaacgac tacgtgcaga cccacgtggc ccggcacgcc ggcctggacc ggtccgccgt
    12661 cgagaaggtg ctcgacgagc tcaccgccct ggccactgcc gccctcgaca ccgagggttt
    12721 cccccgcgag cagcacgagc acgtgcgcac cgccgacctg cgctacttcg gccaggccta
    12781 cgaggtgcgg gtgcccatcg gcgccggccc gctcgacgag gcagcggccg aggcggtcgc
    12841 gcagcggttc cacgacgagc accgccagct ctacggctac gacttccgcg acgacccgcg
    12901 ccagcaggtc gagtgggtca acctgcgggt gaccggcatc ggcccgatca cccggcccga
    12961 gctgcgcgag atcgccccgc gcgatg
//
LOCUS       DAOSOC010000055        12767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4906_length_12767_cov_9.544525, whole genome shotgun sequence.
ACCESSION   DAOSOC010000055 DAOSOC010000000
VERSION     DAOSOC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12767
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4906_length_12767_cov_9.544525"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..1522)
                     /locus_tag="PL014_05970"
     CDS             complement(314..1522)
                     /locus_tag="PL014_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT56672.1"
                     /translation="MDPVRNPYAPGAGQRPPELAGRDEQLDAFDIVLERVARGRPERS
                     IVLTGLRGVGKTVLLNALRSAAVRAGWGTGKLEARPDQSLRRPLASAVHQAVRELGPA
                     GGAGDHALGVVKAFAQREPAGGGTARGAAKLRDRWNPGITVPAVTGRADSGDIEIDLV
                     ELLSDLGGMASDTGRGIAIFIDEMQDLMPDDVSALCAACHELSQSRLPVIVVGAGLPH
                     LPAVLSASKSYSERLFRYSRIDRLDRDAADAALTLPAEDEGATFTPEALDAMYDATGG
                     YPYFIQAYGKAVWDRATASPMTAADVAVAAPEAEAELAVGFFGSRFERATPGEREYLR
                     AMADVAVELGDDSDAVPTADVAAHLGRKPQSLSPARDALLKKGLIYSGERGRIAFTVP
                     HFGRYLRENA"
     gene            1770..2552
                     /locus_tag="PL014_05975"
     CDS             1770..2552
                     /locus_tag="PL014_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HOT56673.1"
                     /translation="MFEAAFWGFVGGVSLIIGAVLGLVFKVTHRTIGLVMGFGAGVLI
                     SAAAFELTMEAYEAAGGPAAAAGLIAGSATFFAGDWLIDRAGGHRRKSPVHGGMPHPR
                     GLDTPPAATAGAVTASSGGAALVLGALLDGIPESAAIGISLLGGAGVSTAMVVAVFLS
                     NIPESMSASTGLRASGRSTTWILALWAGVTAASTISAVLGYTLLGDAPPATLGFIQTF
                     AAGAILTMVADTMVPEAVEHAGRLVGIVTVLGFAVSFFLSAA"
     gene            complement(2637..3497)
                     /locus_tag="PL014_05980"
     CDS             complement(2637..3497)
                     /locus_tag="PL014_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOT56674.1"
                     /translation="MPELPEVQALVDFLRVRTEGLVVTGVELGSISVLKTFSPPPQAL
                     VGAPVDGVARHGKFLDLDCGGTHLVFHLARAGWLRWSDAIPATVLRPGKSPIALRVRF
                     DDGSGFDLTEAGTKKSLAAYIVGDVTAVPRIVSLGPDPLGEDFTLEVFSQIAASHRMQ
                     VKGMLRDQSIIAGVGNAYSDEVLHVAKLSPFALAANLSPDEVERLYEALRGTLAAAVA
                     TASGKPAKELKDAKRAGMRVHGRTGEVCPECGDVVREVSFADSSLQYCATCQTGGKPL
                     ADRRTSKFLK"
     gene            complement(3507..4601)
                     /locus_tag="PL014_05985"
     CDS             complement(3507..4601)
                     /locus_tag="PL014_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HOT56675.1"
                     /translation="MPRPHAAAVLEDAYNERKSGLLRSRGWSTRIVPLTGYGSESQLR
                     VFARVIMTRGEQDDPEEPTHAARLRAVELETRGWRAFFTTPAIDEVVTIRINERITTA
                     RTDRGGFIDLTVRGHGLTPGWHRIFVGSPNAQTIDVPVLVIGNEVTHGIVSDIDDTVL
                     STSLPRPMIAAWNTFLRSEGARKAVPGMATMYRELLAEHPEAPVVYLSTGAWNTAPWL
                     TRFLRRNGYPKGPMLLTDWGPTNTGWFRSGQEHKHAQLHRLARELPHVKWLLIGDDGQ
                     HDPKIYTEFTSRRPGHVRGIAIRELSPGEQVLSHVIPVANDDLVPAPTEELDAPVVRA
                     PDGYGLAPQVRAIWGSASGGGSSAAGGSRG"
     gene            complement(4611..5747)
                     /locus_tag="PL014_05990"
     CDS             complement(4611..5747)
                     /locus_tag="PL014_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT56676.1"
                     /translation="MDDVVSPRLRRWGTRSWLILGVLGMLAAGVWVLAQVSGFVVPLI
                     IAAVLGALFVPVVQWLADRGVPTWGGALAVLLFLAGVVLGSIWLVLAGIVGQYPTIAD
                     QVTAGLGTLDTWLEERGVDVGSGDTLVDSLQAGAGDALGGLGSVLPGVFSGTVSLAIG
                     VAMGAFLVYYVLVDWHSLTTWVGRHMGLDPDTGSAIVGDAVMSVRRYFGSLTLSAVVT
                     AVLIGGAAFAMDVPLAFTIAVITLVTSYIPYLGAIVSGAFATIVALGSNGLTTALILL
                     AVILVVQNVIQTVILARLASTALSLHPIVVLGSTIIGAALAGMLGAALSSPAFAVGVL
                     VHRRLTAASAQEALADGRADAVDPATAPGGPDALGAPPASESVL"
     gene            complement(5770..6051)
                     /locus_tag="PL014_05995"
     CDS             complement(5770..6051)
                     /locus_tag="PL014_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOT56677.1"
                     /translation="MDLATLQDVIERTYGERDRERGVPSTVAWLAEEVGELAQAVRKG
                     TQAQREHEFADVLAWVASLANQMDVDLTAAVQRYATGCPRCAAIPCACS"
     gene            complement(6087..6767)
                     /locus_tag="PL014_06000"
     CDS             complement(6087..6767)
                     /locus_tag="PL014_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOT56678.1"
                     /translation="MATLPLFPLGTVLLPGARLPLQLFEPRYLDLARALAELPDGERC
                     FGVTLIRSGREVGADAVADLYEVGCEARVDAMALAESPIGTVVHLVATGVRRYRLDSL
                     DPDAGTSWTTGHVTFLGEPLPGQPGHGDDPVVADLAARARAEHAAYLRDLGAEAVDLE
                     APTGHLAYRIVEAMVLDPSDRQRVLDGGDPATRLRTVLALLRRERAIVGRFGAVPTPP
                     MPLGPSLN"
     gene            7084..>8388
                     /locus_tag="PL014_06005"
     CDS             7084..>8388
                     /locus_tag="PL014_06005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT56679.1"
                     /translation="MTLSSTTFGALGVPAPLVEILAQREITAPTPIQAATLPDSLAGR
                     DVLGRGRTGSGKTMAFVLPLLTRLSRSTTQRQSRRPRALILAPTRELATQIEDALTPL
                     AAPLGLTSRTVFGGVGQNPQVTALRKGVDIVVACPGRLEDLIGQGHVSLDAVEVTIID
                     EADHMADLGFLPAVRRLLDATPARGQRMLFSATLDAGIGVIAKRYLSNPVTHEADSAQ
                     SPVSTMAHHVLHVTPDGHGQVLLDLVAAPGRTVVFTRTKHRAKKLARQLNGQGVPAVE
                     LHGNLSQNARTRTMEAFHSGAARTLVATDIAARGIHVDDVALVIHADPPVEHKAYTHR
                     SGRTARAGASGTVVTLMTDDQVRDVRDLTRKAGVTATTTRVHATHPLLQELAPGDRSF
                     EGGFAAPVAQASPRQGSGQRRSGSGSGQGSRPASGQGQRRGGP"
     assembly_gap    8389..8488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8613..9329
                     /locus_tag="PL014_06010"
     CDS             8613..9329
                     /locus_tag="PL014_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOT56680.1"
                     /translation="MDWFSEVWARTLPESGVALSGAPVVAVLVAALVAVAFEPLWRIV
                     RLVVTLVHELGHASVGILAGRRFTGFVLRGDMSGHAVTVGPAHGPGRVLTTWAGYPAP
                     AVIGAAAIVLAVRGWSAPVLTVVLAVLVAAALRARSVLTAVVVAVAVIGGGALWWAGS
                     PQLQAQVLIAVGVVLLVGAWRHLGAVITAPDAAASDPGVLARLTRVPRTLWNLTFVIV
                     LAGSTWVAGRAVVTASGLLT"
     gene            9399..9599
                     /locus_tag="PL014_06015"
     CDS             9399..9599
                     /locus_tag="PL014_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HOT56681.1"
                     /translation="MAYQYKVVEVREKMIGGKVSGDRVEKLLNEWAAQGWQLKAITAT
                     DVKGRVGPGGVEGLLVTFERQV"
     assembly_gap    9626..9725
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9837..10295)
                     /locus_tag="PL014_06020"
     CDS             complement(9837..10295)
                     /locus_tag="PL014_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56682.1"
                     /translation="MNTPATPAATTPQGSPGAGLARAVVRWALVLLLVTAGVGHFASA
                     DTFRAQVPPWMPWPEAVIAVSGVIELVLAAALAVAPARLRPTVGWVVAAFFVAIFPGN
                     ISQYLTGTAAFGLDSDAARLVRLLFQPVLVVAALWCTAAWATWRSRRART"
     gene            complement(10307..>12767)
                     /locus_tag="PL014_06025"
     CDS             complement(10307..>12767)
                     /locus_tag="PL014_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HOT56683.1"
                     /translation="ISEYADFSTSFTVAELCVAPTVTIGSVQGSGDATPVGGQSSTVR
                     GVVVGDYEGNTPGLRGFYLQDEGDSDPATSDGIFIFNGGNQNLVEIGDRVAVKGTAGE
                     NQGQTQISTSAANITVCGAGTGSVEPVGITLPMASPTDFEKYEGMLVETTQPLYVTEH
                     FQLGRFGQVVVSSGGRLQQPTNVVAPGPDAAALQAQNNLNRLIIDDASQGQNPDPIVF
                     ARGGNPLSATNTLRGGDLLTEAVGVMTYTWGGNAASPNAYRLRPVGALDASFDVEAAN
                     ERPAAPGEVGGEVRVAAMNLLNYFNTFDGRPDTGPGAVDNCTLGVNGNSTDCRGADDQ
                     EEFDRQWPKTVAAILAVDADVLGVNEIENDGYGGDSSLAHLVGKLNEKAGAGTYAYID
                     ADANIGQTNALGTDAIKVGMLYKPAAVTPIGQTAALNTPEFVGGGDTAPRSRPSLAQA
                     FQVNATGGSFVADVNHLKSKGSACTDPDAGDGQGNCNASRTVSAKALATWLDTDPTGT
                     GEKDVVILGDLNSYAKEDPVKALEAAGYTNLVSSFLGEDAYSYVFDGQWGYLDHALGS
                     ADLFGKNQVAGVAEFHINADEPSVLDYNTDFKTANLQDVLYAPDMFRVSDHDPIIVGL
                     TPNSPATVEAAFDDTSVSCGEGSASLTVDIADRDDADTHEVKIAWGDGTTETVNTASD
                     SLTRTHTYAAAGRYTATVTVTDSHGHVTTTTADVAVEFTTTGVLAPFKKGSVTAKQGS
                     TVPVKVELADCDGSEPTDLDPTLTVSQGSTVARTATLAYVDGKWQHNLSTAGLAPGSY
                     TVTITVPDTGQTITATLVVRR"
BASE COUNT         1785 a         4518 c         4440 g         1824 t
ORIGIN      
        1 cgccgctgaa gtggccgatc tgtcccacct gtcccgaact cgcgagtatt tcccagaatc
       61 gctccccgcc gacaggaagg gctggttcag taagaccgcc gcctacctcg gcaacgcggg
      121 tcgcgacctc gctgtcgaga tcggagcgac cgcgatcaac cggcagatgg ggatgtgaca
      181 ggtcggccgc gtctcaattt ctgcggctgg tgtctcaaag tccaccgctg aagtcgcgat
      241 ctgtctcacg ccgtctcgta ctcccgggta aatctcaaaa tggctcgccg ttgacacgcc
      301 gacagacacg tggtcaggcg ttctcacgca ggtagcgccc gaagtggggc acggtgaacg
      361 cgatccgccc gcgctccccc gagtagatca gccccttctt cagcaatgcg tcacgcgccg
      421 gcgacaacga ctgcggtttg cgcccgaggt gcgccgcgac gtcggccgtg ggcacggcat
      481 ccgaatcgtc gccaagttcg accgcgacat cagccatcgc gcgcaggtac tcgcgctccc
      541 ccggcgtcgc ccgctcgaag cgcgagccga agaacccgac ggccagctcg gcctcggcct
      601 cgggagccgc caccgcgacg tcggccgccg tcatcgggga tgccgtagcg cggtcccaga
      661 ccgccttgcc gtacgcctgg atgaagtagg ggtagccgcc ggtcgcgtcg tacatcgcgt
      721 cgagcgcctc gggagtgaag gtcgccccct cgtcctcggc cgggagcgtg agggcggcgt
      781 ccgcggcatc ccgatcgaga cggtcgatgc gcgagtagcg aaagagacgc tccgagtacg
      841 acttcgacgc cgacaagacc gccggcaggt gcggcagccc ggcgccgacg acgatgaccg
      901 gcagccgcga ctgcgacagc tcgtggcagg ccgcgcacag ggcggagacg tcgtcgggca
      961 tgaggtcctg catctcgtcg atgaagatcg cgatgccccg gccggtgtcg gacgccatgc
     1021 cgccgaggtc gctcagcagc tcgacgaggt cgatctcgat gtcgccggag tcggcccgcc
     1081 cggtgaccgc gggaacggtg atgccggggt tccaccggtc acgcagcttc gcggcccccc
     1141 gggcggtgcc cccgcctgcg ggctcgcgct gggcgaacgc cttgacgacg ccaagggcgt
     1201 ggtcccccgc cccaccggcc gggccgagct cgcgcaccgc ttggtgcacg gccgaggcga
     1261 gcggccgacg cagcgactgg tcgggccggg cctcgagctt gccggtgccc cagccggcgc
     1321 gcaccgcggc cgagcgcagg gcgttgagca gcacggtctt gccgacaccg cgcagcccgg
     1381 tgagcacgat cgagcgctct ggccgacctc gggccacccg ctcgagcacg atgtcgaacg
     1441 cgtcgagctg ctcgtcacgg ccggcgagct cgggcggtcg ctgaccggcg ccgggggcgt
     1501 aggggttcct gaccgggtcc atgatcggag ggtatacggc tctctagcgt tccccgcata
     1561 catcgggata gagcggtatc gcgtgtcgcc gcgagcggcc ggtcgcctcc agcggaccgg
     1621 ccgacaggca atggcgaggg catggcttcc gcgcgagcac gggccgcgcc ggtggcgggc
     1681 cacggggccc ggcactgcct gtccgtgggt acgcccctga ccctggccac ccggcccgcg
     1741 ccgagaccga gcgcagtagc gtgacggacg tgttcgaggc tgcgttctgg ggtttcgtcg
     1801 gtggtgtctc gctcatcatc ggcgcggtgc tcgggctcgt cttcaaggtc acgcaccgca
     1861 cgatcgggct cgtcatgggc ttcggcgccg gggtgctcat cagtgccgcc gccttcgagc
     1921 tcaccatgga ggcgtacgag gcggcaggtg gcccggccgc ggcagcgggt ctcatcgccg
     1981 gctcggccac cttcttcgcg ggtgactggc tcatcgaccg cgcgggcggt caccgccgca
     2041 agagccccgt gcacggcggg atgccgcacc cgcgcggcct ggacactcct ccggcagcca
     2101 cggccggtgc cgtgacggcg agctcgggcg gcgccgctct ggtcctggga gccctgctcg
     2161 acggcatccc ggagtccgcg gccatcggca tcagcctgct cggcggcgcc ggggtgagca
     2221 cggccatggt cgtcgcggtc ttcctctcca acatccccga gtcgatgtcc gcgtcgacgg
     2281 gactgcgcgc ctcaggccgg tcgaccacct ggatcctcgc cctgtgggct ggggtgaccg
     2341 cggcatcgac gatctcggcg gtgctcggct acaccctgct cggcgacgcg ccgccggcga
     2401 ccctggggtt catccagacc ttcgccgcag gggcgatcct gacgatggtc gccgacacca
     2461 tggttcccga ggccgtcgag cacgcgggcc ggctcgtcgg catcgtcacc gtgctggggt
     2521 tcgccgtgtc gttcttcctc agcgccgcct gacccggtcg gcaccggccc tgtctagcgc
     2581 ccaggacata cagcctgata cgccgacatc gacagtccgg tcggtgagcc gaccggctac
     2641 ttgaggaact tgctcgtgcg ccgatcggcc agcggcttgc ccccggtctg gcaggtcgcg
     2701 cagtactgca gcgacgagtc ggcgaacgac acctcgcgca ccacgtcgcc gcactcgggg
     2761 cagacctcac cggtgcgccc gtgcacccgc atccccgcgc gcttggcgtc cttgagctcc
     2821 ttggcaggct tgccggatgc cgtggccacc gcggcggcca gcgtcccccg aagtgcctcg
     2881 tacaacctct cgacctcgtc cggcgacagg ttggccgcga gggcgaacgg cgacaacttc
     2941 gccacgtgca gcacctcgtc ggagtaggcg ttgccgacgc cggcgatgat cgactggtcg
     3001 cgcagcatcc ccttgacctg catgcggtgc gacgccgcga tctgtgagaa gacctcgagc
     3061 gtgaagtcct caccgagcgg gtcaggcccg agcgagacga tgcgcggcac cgcggtgacg
     3121 tcgccgacga tgtaggccgc gagcgacttc ttcgtgcccg cctcggtgag gtcgaagccc
     3181 gagccgtcgt cgaagcgcac ccgcagcgcg atcggtgact tgcccggtcg cagcaccgtg
     3241 gccgggatcg cgtcggacca gcgcagccac ccagcccggg ccaggtgaaa caccaggtgc
     3301 gttccgccgc agtcgaggtc gaggaacttg ccgtgccggg cgaccccgtc gaccggggcg
     3361 ccgacgaggg cctgcggcgg tgggctgaac gtcttcagca ccgagatgga gccgagctcg
     3421 accccggtga cgaccaggcc ctcggtgcga acacgcagga agtcgacgag ggcctgcacc
     3481 tcgggaagct cgggcatagc cggaacctac ccccggctgc cgccggccgc actcgaccca
     3541 ccaccagacg cgctccccca gatggctcgc acctgcgggg ccaacccgta gccgtcgggc
     3601 gctcgcacca cgggcgcgtc gagctcctcg gtcggcgccg gcacgaggtc gtcgttggcg
     3661 acggggatga cgtggctgag cacctgctcg cccggcgaca gctcacggat ggcgatgccc
     3721 cgcacgtgcc cgggtctgcg cgacgtgaac tcggtgtaga tcttcgggtc gtgctggccg
     3781 tcgtccccga tgagcagcca cttcacgtgc ggcagctccc gagccaaccg gtgcagctgg
     3841 gcgtgcttgt gctcctgccc cgagcggaac caaccggtgt tcgtcgggcc ccagtcggtg
     3901 agcagcatcg gccccttcgg gtagccgttg cgccgcagga accgggtcag ccagggcgcg
     3961 gtgttccacg cgcccgtcga caggtagacg accggcgcct ccgggtgctc ggcgagcagc
     4021 tcccggtaca tcgtcgccat gcccggcacc gccttgcggg caccctcgga acgcaggaag
     4081 gtgttccacg ccgcgatcat cggccgcggc agcgacgtcg agagcacggt gtcgtcgatg
     4141 tcggacacga tcccgtgcgt cacctcgttg ccgatcacga gcaccggcac gtcgatggtc
     4201 tgcgcgttcg gcgacccgac gaagatgcgg tgccaccccg gcgtcaggcc gtggccacgc
     4261 acggtgaggt cgatgaatcc tccgcggtcg gtgcgggccg tggtgatccg ctcgttgatc
     4321 cggatcgtca cgacctcgtc gatcgccggt gtcgtgaaga acgcccgcca cccgcgcgtc
     4381 tcgagctcga ccgcccgcag ccgcgccgcg tgcgtcggct cctcggggtc gtcctgctca
     4441 ccgcgggtca tgatcacccg ggcgaacacc cgcagctgcg actcgctgcc gtacccggtg
     4501 agcggcacga tccgcgtgct ccacccccgt gaccggagca ggccggactt gcgctcgttg
     4561 taggcgtcct cgagcactgc tgcggcgtgt ggtcgcggca tgccgaaagc ctagaggacg
     4621 ctctcggatg ccggtggcgc gccgagcgca tccggcccac cgggcgcggt ggcagggtca
     4681 acggcatccg cccgtccgtc ggcgagcgcc tcctgggctg acgccgcggt gagccgacgg
     4741 tgcaccagga cgccgacggc gaatgccggg gacgacagcg ccgccccgag catgcccgcc
     4801 agggctgcgc cgatgatcgt ggagccgagc acgacgatcg ggtgcaggct gagcgcggtg
     4861 ctcgccaggc gcgcgaggat gaccgtctgg atgacgttct gcacgacgag gatgacggcg
     4921 agcaggatca gcgcggtggt gaggccgttc gagcccaggg ccacgatcgt cgcgaacgcc
     4981 ccggagacga tcgccccgag gtaggggatg tacgacgtca cgagggtgat gacggcgatg
     5041 gtgaaggcga gcggcacgtc catggcgaag gcggcaccgc cgatgagcac ggccgtgacg
     5101 acggccgaca gcgtcaggct gccgaagtag cggcgcaccg acatcacggc atccccgacg
     5161 atggccgacc cggtgtcggg gtccaggccc atgtgacggc cgacccaggt ggtcagggag
     5221 tgccagtcga cgagcacgta gtacacgagg aaggcgccca tcgcgacacc gatggccagc
     5281 gacaccgtgc ccgagaagac cccgggcagc accgacccga gcccgccgag cgcgtcaccg
     5341 gcacccgcct ggagcgagtc gacgagggtg tcgccggagc cgacgtcgac cccccgctcc
     5401 tcgagccagg tgtcgagcgt gccgaggccg gcggtgacct ggtcggcgat cgtcgggtac
     5461 tgcccgacga tgcccgcgag gacgagccag atcgacccga gcaccacccc ggcgaggaag
     5521 agcagcaccg cgagcgcccc gccccacgtg ggcactcccc ggtcggccag ccactgcacg
     5581 acggggacga acagcgcacc gagcaccgcg gcgatgatga gcggaacgac gaaaccggac
     5641 acctgcgcga gcacccacac cccggcagcg agcatgccga gcaccccgag gatcagccag
     5701 gaccgggtgc cccatcggcg caggcggggc gagacgacgt cgtccacggg ggtgcctctc
     5761 gatgggtggt caggagcagg cacagggtat cgctgcgcag cgcgggcacc cggtggcata
     5821 gcgctgaacg gcagcggtca ggtcgacgtc catctggttc gcgagcgagg ccacccaggc
     5881 cagcacatcg gcgaactcgt gctcgcgctg ggcctgcgtg cccttgcgca cggcctgggc
     5941 gagctcaccg acctcctcgg cgagccaggc caccgtgctg ggaaccccgc gctcgcggtc
     6001 gcgctcgccg taggtgcgct cgatgacgtc ctggagggtg gcgaggtcca tgcgcccgaa
     6061 cctacccggg gcttctccag ctggggtcag ttcagggagg ggccgagcgg catcggcggg
     6121 gtcggcacgg cgccgaaacg accgacgatc gcccgctccc gccgcagcag ggccagcacg
     6181 gtgcgcagcc gagtcgccgg gtcgccaccg tcgagcacgc gctgccggtc actcgggtcg
     6241 agcaccatcg cctccacgat ccggtacgcc aggtgcccgg tcggcgcctc gaggtcgacc
     6301 gcctcggcac cgaggtcacg caggtatgcc gcgtgctcgg ctcgcgctcg tgccgccagg
     6361 tcggcgacca ccgggtcatc gccgtgccct ggctgcccgg gaagcggctc accgaggaag
     6421 gtgacgtgac ccgtggtcca cgacgtgccg gcatccggat cgagcgaatc caaccggtag
     6481 cgccgcaccc cggtggcgac gaggtggacc accgtgccga tcgggctctc cgcgagggcc
     6541 atcgcgtcga cgcgcgcctc gcagccgacc tcgtagaggt cagcgacggc atccgccccg
     6601 acctcgcgcc ctgatcggat gagggtgacc ccgaagcagc gttcgccgtc gggcagttcg
     6661 gcgagcgcgc gggcgaggtc gaggtagcgc ggctcgaaca gctgcagggg cagtcgtgcg
     6721 ccggggagca gcaccgttcc gagggggaag agcggcaagg tcgccatgcc ccactgtagg
     6781 caccgacccc cacctctccc cgactcattg cactccagcc acctcaccca ggtggctgac
     6841 gtgcaagaag tcgcgcagga ggccgatgcg gaggttcgac agctcacccg ctacagtgga
     6901 cgtgctggcc cgccttctgc ctcgggccgc aatctttgat gtcgatgcct tcgcgtcggg
     6961 gccgcaagcc ctcgactcgg cgccgcggcc cagagccccg cccggcagaa catcccgtgt
     7021 cgctgacacc gttgatggtc aatgccgacc gcagcactgc gcgaccgccc tgaactcaaa
     7081 ggaatgacct tgtccagcac cactttcggc gcgctcggcg tgcccgcccc cctcgtcgag
     7141 atccttgccc agcgcgagat caccgccccc acccccatcc aggccgcgac cctgcccgac
     7201 tccctcgcgg gtcgcgacgt cctcggccgt ggccgcaccg gctcgggcaa gacgatggcg
     7261 ttcgtcctgc ccctgctgac ccgcctgtcg cgcagcacga cgcagcgcca gtcgcgtcgc
     7321 ccgcgcgccc tcatcctggc cccgacccgc gagctcgcga cccagatcga ggacgccctc
     7381 accccgctcg cagcccccct cggcctgacc tcgcgcaccg tcttcggtgg cgtcggccag
     7441 aacccccagg tcacggccct gcgcaagggt gtcgacatcg tcgtcgcctg ccccggccgc
     7501 ctcgaggacc tcatcggcca gggtcacgtc tccctggatg ccgtcgaggt cacgatcatc
     7561 gacgaggccg accacatggc cgacctcggc ttcctgcccg ccgtgcgccg cctgctcgac
     7621 gccacccccg cgcgcgggca gcggatgctg ttctccgcca cgttggatgc cgggatcggc
     7681 gtcatcgcga agcgttacct ctcgaacccg gtcacccacg aggccgactc cgcgcagtcg
     7741 ccggtgtcga ccatggccca ccacgtgctg cacgtgacgc ccgacggtca cggccaggtg
     7801 ctgctcgacc tcgtcgccgc ccccggccgc accgtggtct tcacccgcac caagcaccgc
     7861 gccaagaagc tggcccgcca gctcaacggc cagggcgtcc ccgcggtcga gctgcacggc
     7921 aacctcagcc agaacgcgcg cacccgcacg atggaggcct tccactcggg tgcagcccgc
     7981 accctggtcg ccaccgacat cgcggcgcgc ggcatccacg tcgacgacgt cgccctcgtc
     8041 atccacgccg acccgccggt cgagcacaag gcctacacgc accgctcggg ccgcaccgcc
     8101 cgcgccggtg cctccggcac ggtcgtcacg ctgatgaccg acgaccaggt gcgcgacgtg
     8161 cgcgacctca cccgcaaggc cggggtcacg gccacgacca cccgcgtgca cgccacgcac
     8221 ccgctgctcc aggagctcgc ccccggcgac cgctccttcg agggtggctt cgctgctccc
     8281 gtggctcagg cgtcgccgcg ccaggggtcg ggccagcgcc gctccggctc cggctcgggt
     8341 cagggctctc gcccggcctc gggtcagggt cagcgtcgtg gcggcccgnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta ggtcgatctc ggtacgctcc cgcccaccaa
     8521 gccgtccccc gagtgaccta ttggtcgatc ggggtacgtg tgtgtcgacc cggcactgac
     8581 gtccacaggg tcaccgaata cggtctcgcg gcatggactg gttcagcgag gtgtgggccc
     8641 gcaccctgcc cgagagcggg gtggcgctgt ccggtgcccc cgtcgtcgcc gtgctcgtcg
     8701 cagcgctggt ggccgtggcg ttcgagccgc tctggcgcat cgtgcgcctt gtcgtgaccc
     8761 tcgtgcacga actcggccac gcgagcgtcg gcatcctcgc cgggcgccgg ttcaccgggt
     8821 tcgtcctgcg cggcgacatg tcgggccacg ccgtgaccgt cggcccggcg cacggccccg
     8881 gccgcgtgct cacgacctgg gccggctacc cggcaccagc cgtcatcggg gcggccgcga
     8941 tcgtgctcgc cgtgcgcggc tggtcggccc cggtgctcac ggtggtcctc gcggtgctgg
     9001 tcgccgcggc cctgcgggcc cgatcggtgc tgaccgccgt cgtcgtcgcc gtcgccgtca
     9061 tcggcggcgg agccctgtgg tgggccgggt ccccgcaact ccaggcccag gtgctcatcg
     9121 ctgtcggggt ggtgctcctc gtcggggcgt ggcgccacct cggggccgtc atcaccgccc
     9181 cggatgccgc cgcgagcgac cccggcgtgc tcgctcgcct cacgcgcgtc ccccggaccc
     9241 tgtggaacct gacgttcgtc atcgtgctgg ccggctccac gtgggtcgcg ggccgggccg
     9301 tcgtgacggc atccggcctg ctcacctgac gcagtagggg gggcagacgc gcgccctgcc
     9361 gcgcccccgg catcccgctg cgggtctacg gtgggggcat ggcataccag tacaaggtcg
     9421 tcgaggtccg tgagaagatg atcggcggca aggtctcagg tgaccgcgtc gagaagctgc
     9481 tcaacgagtg ggctgcgcag gggtggcagc tcaaggccat caccgcgacg gacgtcaaag
     9541 gccgcgtcgg cccgggcggt gtcgaggggc tcctcgtcac cttcgagcgc caggtctgac
     9601 ccgtacccag atcgaccaat aggtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnntcctc gtcaccttcg agcgccaggt ctgacccgta cccagatcga ccaataggtc
     9781 accacaggta catcaccgcc cgaaccggcc gtacccagat cgaccaatag gtctccttag
     9841 gtacgcgccc gccgcgagcg ccaggtcgcc cacgcagccg tgcaccacag ggcggcgacg
     9901 acgagcaccg gctggaagag caggcgaacc aggcgtgcgg catccgagtc gaggccgaag
     9961 gccgctgtgc ccgtgaggta ctggctgatg ttgcccggga agatcgccac gaagaacgcc
    10021 gccaccaccc aacccacggt ggggcgcagc cgcgcgggag cgacggcgag cgcagcggcg
    10081 aggaccagct cgatcacccc actgacggcg atcaccgcct cgggccacgg catccacggc
    10141 ggtacctggg cgcgaaaggt gtcagccgac gcgaagtgac ccacccccgc cgtcacgagg
    10201 agcagcacga gcgcccagcg caccaccgca cgcgcgaggc ccgcacccgg cgacccctgc
    10261 ggcgtcgttg ccgcaggggt cgccggggtg ttcatggacg agtccgtcaa cgccgcacga
    10321 caagcgtggc ggtgatggtc tgaccggtgt cgggaacggt gatcgtcacc gtgtagctac
    10381 ccggtgccag gcccgcggtc gacaggttgt gctgccactt gccgtcgacg taggcgaggg
    10441 tcgcggtgcg cgccacggtg ctgccctggc tcacggtgag cgtcgggtcg aggtcggtcg
    10501 gctcggaccc gtcgcagtcg gcgagctcga ccttgaccgg caccgtcgaa ccctgcttgg
    10561 ccgtgacgct gcccttcttg aacggggcga gcacgccggt cgtcgtgaac tcgacggcga
    10621 cgtcggcggt cgtcgtcgtg acgtggccgt ggctgtcggt gaccgtgacg gtcgccgtgt
    10681 agcgcccggc tgcggcgtag gtgtgcgtgc gggtgagcga gtcggatgcc gtgttcaccg
    10741 tctcggtggt gccgtcaccc caggcgatct tcacctcgtg ggtgtcggcg tcgtcccggt
    10801 cagcgatgtc gaccgtgagg ctggcgctgc cctcaccaca gctcaccgac gtgtcgtcga
    10861 aggctgcctc gacggtggcc ggggagttcg gcgtgaggcc gacgatgatc gggtcgtggt
    10921 cggagacgcg gaacatgtcc ggggcgtaga gcacgtcctg gaggttggcc gtcttgaagt
    10981 cggtgttgta gtccagcacc gacggctcgt cggcgttgat gtggaactcc gcgacaccgg
    11041 cgacctggtt cttgccgaac aggtcggccg aaccgagggc gtggtcgagg tagccccact
    11101 ggccgtcgaa gacgtaggag taggcgtcct caccgaggaa gctgctcacg aggttggtgt
    11161 accccgcggc ctcgagcgcc ttgaccgggt cctccttggc gtaggagttc aggtccccga
    11221 ggatgacgac gtccttctcg cccgtgcccg tcgggtcggt gtccaaccac gtggccaggg
    11281 ccttggccga gacggtgcgc gaggcgttgc agttgccctg cccgtcaccg gcgtcggggt
    11341 cggtgcaggc cgagcccttg gacttcaggt ggttgacgtc ggcgacgaac gagccgccgg
    11401 tggcgttgac ctggaaggcc tgggccagtg aggggcggct gcgcggggcg gtgtcgccac
    11461 ccccgacgaa ctcgggggtg ttgagcgccg cggtctgccc gatcggggtg acggccgccg
    11521 gcttgtagag catgccgacc ttgatggcgt cggtgccgag ggcgttggtc tgaccgatgt
    11581 tggcgtcggc gtcgatgtag gcgtaggtgc ccgcgccggc cttctcgttg agcttgccga
    11641 cgaggtgcgc cagcgagctg tcgccgccgt agccgtcgtt ctcgatctcg ttgactccga
    11701 ggacgtcggc gtcgaccgcg aggatcgctg ccaccgtctt gggccactgc cggtcgaact
    11761 cctcctggtc gtcggcgccg cggcagtcgg tggagttgcc gttcacgccg agggtgcagt
    11821 tgtcgaccgc gccgggaccg gtgtccggcc gtccgtcgaa ggtgttgaag tagttgagca
    11881 ggttcatcgc cgcgacgcgg acctcaccac cgacctcgcc cggggcggcc gggcgctcgt
    11941 tggccgcctc gacgtcgaac gaggcgtcga gggcaccgac ggggcgcagg cggtaggcgt
    12001 tggggctcgc cgcgttgcct ccccaggtgt aggtcatgac cccgacggcc tcggtgagca
    12061 ggtcgccacc gcgcagggtg ttggtcgcac tgagcgggtt gccgccgcgg gcgaagacga
    12121 tcgggtcagg gttctgcccc tgggaggcgt cgtcgatgat gaggcggttg aggttgttct
    12181 gcgcctgcag agccgccgcg tccgggccgg gggcgacgac gttggtcggc tgctggaggc
    12241 ggccaccgct ggagacgacg acctggccga agcggccgag ctggaagtgc tcggtgacgt
    12301 acagcggctg ggtggtctcg acgagcatcc cctcgtactt ctcgaagtcc gtggggctcg
    12361 ccatcggcag ggtgatgccg accggctcaa cgctgcccgt gcccgcgccg cagaccgtga
    12421 tgttggccgc gctcgtcgag atctgggtct ggccctggtt ctcaccggcc gtgcccttga
    12481 cagcgacccg gtcgccgatc tcgacgaggt tctggttgcc gccgttgaag atgaagatgc
    12541 cgtcggaggt ggcggggtcg ctgtcgccct cgtcctggag gtagaagccg cgcaggcccg
    12601 gggtgttgcc ctcgtagtcg ccgacgacga caccgcgcac ggtgctcgac tggccgccga
    12661 ccggggtggc gtcgccggaa ccctgcaccg agccgatggt cacggtgggg gccacgcaca
    12721 gctcggccac ggtgaaggat gtggagaagt cggcgtactc cgagatg
//
LOCUS       DAOSOC010000056        12765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4907_length_12765_cov_8.185995, whole genome shotgun sequence.
ACCESSION   DAOSOC010000056 DAOSOC010000000
VERSION     DAOSOC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4907_length_12765_cov_8.185995"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="PL014_06030"
     CDS             <1..1436
                     /locus_tag="PL014_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56684.1"
                     /translation="EVTDWVASNGAASSYGREYRYDGAGRLALARDNAATVDPATADL
                     TSTCTDRAYTFNTNGNRTQLATTTRPGGDCATAGTTTTVTTTGWDTADRATTGRNGTG
                     SYVYDLFGRQTTLPSSDAPNPAGGNITLGYFDDDLPRTVTQGGTSTTFTLDANGRRST
                     QTTVDGSGTTTTVRRYTDGSDNPAWVDTTPPGAGTTTTRFTEAIGGDLSASIASDGGL
                     IISLGNPHGDVVTTITIPDNQEADPPATAISGWATYDEYGNTTTADAVDGTLGYGWLG
                     TKQRSTSTATAGLTLMGVRFYNAARGLFTSLDPIPGGNDTPYTYPTDPINMFDLDGRK
                     SRWRTFWKRAATATGILAAGACIVATAGLCAGAAVLAAGASAGWAVYQKRKGTRKERA
                     RSWRSYGASVALDVASVFFRPVRSVKYLRHVSRHRGATRYGRYYGRRARTQTRSLNWH
                     VHNRRRSTAFWSGVNVGMMHRSYRNGW"
     gene            1955..2248
                     /locus_tag="PL014_06035"
     CDS             1955..2248
                     /locus_tag="PL014_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT56685.1"
                     /translation="MALDVELSETRDFLACHEPFDALPQGVLDRLPGRMSVQYFRRGT
                     RLIEKGRDNHHLYVLRSGAADVHDPQGTLVDRGGEGACFGSITLTEGNTRRSR"
     gene            complement(2343..3578)
                     /locus_tag="PL014_06040"
     CDS             complement(2343..3578)
                     /locus_tag="PL014_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT56686.1"
                     /translation="MVDLELRERLTSIGAVLIEGPKACGKTATATQVAATMHRLDVDQ
                     AARNTATYAPDVLLDSPGPVLLDEWQRAPALWDHVRRAVDDRSPEKGLFIMTGSATPR
                     DDVNRHSGAGRIAVVQMRPMSLWESGHSSGTASLASLMDGGRVAGRDSGPSLPDIIDR
                     ICIGGWPALLDATATEAGRWLRDYLNQIVLVDIQELGTRRRDPQNIRRLLTSLARNVA
                     TSARVTALAKDVGGADGPVARATVDGYLDSLHRLRLIENSSAWGPHMRSATPLQASPT
                     RYFIDPSLAVAALGQGPQQLLRDLNATGYLFENLAVRDMRVYAQRLGGEVHHWRDQNQ
                     NEVDIVVTLPDGRWGAFEVKMNPEDADKAAASLLSFSEKVDQAKAGAPVALGVITTTG
                     FAYRRDDGVSVLPIGAMGP"
     gene            3894..5765
                     /locus_tag="PL014_06045"
     CDS             3894..5765
                     /locus_tag="PL014_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HOT56687.1"
                     /translation="MALDVELTETRDFLARHEPFDALPQSVLDRLPARMTVQYFRRGS
                     RLIEKGRDNHHLYIVRSGAADVHDPQGTLVDRGGEGACFGSITLTQGNPSTFDVTARE
                     DTLALVLPADDFHRLCADQPEFDAFFDAQRASRMSGAVASVQLSSTGGAILKTTVRDL
                     IAREPIGVDARVSVRDAARLMSTERVSALLVTRDGRLAGILTDRDLRTRVLAVDVDPA
                     TPVADVMTADPVTGSADALAFEVLLEMVGRHIHHLPILDAHDQPVGLVTTTDLLRLEQ
                     SNPVYLAGDIAKQADVAGVARAASRLPQVVLSLVEQDASADDIGRVVTAVGDAVERRL
                     LALAEAELGPPPVPYCWVSLGSRARLEQALAADQDTAMIIDDAMLPEHEPWFAALAER
                     VTADLAACGYPRCTGDVMATNPRWRQPLASWRRQFTSWLTEPVPDAILGASIFFDMRP
                     VHGDATLEARLQRHVLTASPDAKVFLAHLAKQATANEPPLGFFRGFVLEKAGSHENTL
                     DIKRGGIGAVVELARVHALSLGSPAVNTRARLDAAHAAGVLGDDRAADLRDAFEFISY
                     VRLRHQAAQVRAGLPPDNHVAPDDLSSFDKRHLREAFAIVRQAQTALAHRYPTHYIS"
     gene            5762..6475
                     /locus_tag="PL014_06050"
     CDS             5762..6475
                     /locus_tag="PL014_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOT56688.1"
                     /translation="MSLFRRTPDERRQRALRSAAPGPLRDYLGVPFPAPSVPLDELPL
                     LAVDIETTGLDPRVDRVLSIGWVPVDGGSVVLGGAGRAVVRDQVGVAGEAGVGSSATI
                     HGLTDDALAGGEVMADAVARLLGALTGRVLLAHFARIETTFLSGACERLWGAPLPCVV
                     VDTLDLEHRVVTGRGQRDPEPGALRLWSARERHGLPVYPAHEALVDALACAELYLAQR
                     AELRAARPETTLTLRQVMA"
     gene            complement(6544..6711)
                     /locus_tag="PL014_06055"
     CDS             complement(6544..6711)
                     /locus_tag="PL014_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56689.1"
                     /translation="MNAASLSLQKARSLASALRTSRHHGRGWTEHVDRDLVRESQELY
                     LLAQSRSPRMF"
     gene            6793..7704
                     /locus_tag="PL014_06060"
     CDS             6793..7704
                     /locus_tag="PL014_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT56690.1"
                     /translation="MLDPHRLRVFRSVVASGSVQAAADNLGLTSSAVSQHVAALQRET
                     GLTLFHRAGRGIVPTEAALALVERTDEVMSRWGELDQAVADLREGRSGRLAIGYFASA
                     GAAWMPALVKRLTHEYPDLVLELVLTEAEARGSSTGRGPDIDLVIDPPDTPVRSGYRR
                     IELTTDPFVVIVPAGHPLIGQGPVSLSDLRRETWVSNDYPRSIGHRMVVAHCNAVGFR
                     PRFTVQAQDHYTAIRFVEAGIGVSVLPGLAARDLPASVVRIPLAPPAPVRHLAALVRD
                     DGTPDEAADRAVALLTDLIHHPSSRLR"
     gene            complement(7718..10297)
                     /locus_tag="PL014_06065"
     CDS             complement(7718..10297)
                     /locus_tag="PL014_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT56691.1"
                     /translation="MSTALYRLGRWCATHARRVLVLWLVVLVGAGALAATISQPLGNG
                     FTIPGANFEKVRQQLGEEIPKAAGGFGTVVLSSDTAFTPAQRAAVEDVFAAWGEVPNV
                     SRVINPFEAQDQLDAGQAQLADAKVQLDAGQKGIDEGQAKLAEGEGQLAAGEAVLAQL
                     EETNPQDPSIPGLRAQLTQGRAELEKGRAELEKGIAELAAGRASYEDGVAISRATEGT
                     RLVSEDGRYAVAQVQFDTDAQSVPVENRAMIPDTAKDALAAAGVTADYSVEITQDTAL
                     IGAGEIIGLTVALLVLVAVLGSLIAAGLPLLVALLGVGVGIGGAIAFTAVTELNQMTP
                     ALALMLGLAVGIDYALFIVTRHRTSLLHGQELVESISRAVGTAGSAVVFAGLTVVIAL
                     TALVLSGLPILAEMGLVAAFTVAVTVLVAVTVSPALLRLMGTRVISRRGWRAAGFATP
                     GDPTSRTTPDDGTDEEHGGWFVRLVTLRPWLTVLAVVGVLGVLAIPVGSIQLGLPDGG
                     REPTGTTAHTAYTTVGEQFGPGINGPIVAVTTLPEELRPTDEASLANVQAMIATRLKV
                     IEGVRSVVPFGVSPDQATLAFQVVPTTGPADPATAETFHRVEFTASQLERRENIKLGL
                     TGQTVANIEVSERLAAALPAYLAVVVGLSVIILLLVFRSIVVPLIATGGFLLSIAAAF
                     GATVAVHQWGWLGALFGVHQAGPLLSVMPIIIIGVLFGLAMDYQMFLVSGMHEARSHG
                     EDSRTAVRTGFVHGAKVVTAAAIIMTSVFLGFAFSHLAMVRPIGFALAVGVLLDAVLI
                     RMTLTPALMHLLGDRAWYLPRWLDRILPDLDVEGVRLAERLDSGALGSVTPAPPTPVP
                     TTT"
     gene            complement(10297..>10728)
                     /locus_tag="PL014_06070"
     CDS             complement(10297..>10728)
                     /locus_tag="PL014_06070"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56692.1"
                     /translation="FTASVAAFFDSVGERLDARPDDEDVLDSAVAVITDPGDVELLQR
                     IGVLSAAGESSPHARGLILVEAHTWLDWLEEWLRRRLPPGTDDLLVATFAATMLGAAE
                     AAFRVWAKAPSGELSAAVARALDHLRTGLDPATPTPTPEDH"
     assembly_gap    10729..10828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10829..12097
                     /locus_tag="PL014_06075"
     CDS             <10829..12097
                     /locus_tag="PL014_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT56693.1"
                     /translation="PYEHHSNEIPWRESIADVVTIREDADGGVCRDDLRARLEEYADR
                     PLKIGSFSAASNVTGIVSDTHGVSALLHEHGALAFWDFAAAAPYVDIEMTAPVSGDPA
                     AYKDAIFLSPHKFIGGPSTPGVLIARRELFGNRVPVVPGGGTVAYVNPEDHAFVTDAA
                     HREEGGTPAIIESVRAGLVFQLKEAVGVETIRAHEERLLSRAVEAWRAEPAIEILGNL
                     DAPRLSIVSFVVRAPSGHWLHHNFVVALLNDLFGIQARGGCSCAGPYGHRLLGIDIER
                     SHEFEREITGGCEGIKPGWVRVNFNYFISDTVADYVVDAVRLVARDGWRLLGDYIFDP
                     ETGRWRHRDGVVEPPIRLGAISYADDGSMRVPRTDNRGGEELLAQHLADGAAILAAAI
                     PPDLGSHASGLSDDFEHLLWFDLPTGALGS"
     gene            12121..>12765
                     /locus_tag="PL014_06080"
     CDS             12121..>12765
                     /locus_tag="PL014_06080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT56694.1"
                     /translation="MQPVGAPVTGWTARPLPAGVVLTGRTCLLEPLSSAHVAGLAEAW
                     AGTDDAHWTYLPMPRPHTPDAVAHLVDSMVGDPTTVAYCVLVDALAAGVLSLMRIDPA
                     NGVVEIGAVILGTSLVRTLASTEAQRLLMGHVFDDLGYRRLEWKCDALNAPSRRAALR
                     LGYTFEGVFRQALVVKGRNRDTAWFSVIDGEWPAVRERLDAWLDPANFDAAGRQR"
BASE COUNT         1872 a         4466 c         4440 g         1887 t
ORIGIN      
        1 ccgaggtcac cgactgggtg gcatccaacg gtgccgcgtc ctcgtacggg cgtgagtacc
       61 gctacgacgg tgcgggccgg ctggcactgg cccgggacaa cgccgccacg gtcgacccag
      121 ccaccgcaga cctgaccagc acgtgcacgg accgcgcgta cacgttcaac accaacggca
      181 accgcaccca actcgccacg accacccgcc cgggcggtga ctgtgccacc gcgggcacca
      241 ccaccacggt caccacgact gggtgggaca ccgccgaccg ggccaccacc ggccgtaacg
      301 ggaccgggtc gtacgtgtac gacctgttcg ggcggcagac caccctgccg agctctgacg
      361 cgcccaaccc cgccggcggg aacatcaccc tgggctactt cgacgacgac ctcccccgca
      421 cggtcaccca gggcggaacg tcgacgacgt tcaccctcga cgctaacggg cgccgctcca
      481 cccagaccac cgtcgacggt tcgggcacca cgaccacggt gcgccggtac accgacggtt
      541 cggacaaccc cgcctgggtc gacaccacgc cccccggtgc cggcacgacc acgacacggt
      601 tcaccgaagc catcggtggc gacctgtccg ccagcatcgc ctccgacggc ggcctcatca
      661 tctccttggg taacccccac ggcgacgtcg tcaccaccat caccatcccc gacaaccagg
      721 aagcggaccc ccccgccacc gccatcagcg ggtgggccac ctacgacgaa tacggcaaca
      781 ccaccaccgc ggacgccgtc gacggcaccc tcggctacgg ctggctcgga accaaacaac
      841 gatccaccag caccgcaacc gccggtctga cgctgatggg agtgcggttc tacaacgcgg
      901 cgaggggact gttcacctcc ctcgacccca tccccggagg caacgacacc ccctacacct
      961 accccaccga ccccatcaac atgttcgacc tcgatggaag gaaatcgagg tggcggacgt
     1021 tctggaagag ggcggccacc gcgacgggca ttctggcggc cggggcctgc atcgtcgcaa
     1081 cagctggcct atgcgctggg gccgcggtcc ttgctgcggg cgccagcgcg ggttgggcgg
     1141 tgtaccagaa gagaaagggc acgcgaaagg agcgggcaag aagttggcgt tcatacggtg
     1201 cgagcgtcgc cctagatgta gcttcggtgt tctttcgccc cgtgcgtagc gtcaagtact
     1261 tgagacatgt atcgcgacac cgaggtgcga cgcgctacgg ccgctactac ggaaggcggg
     1321 caaggacgca aacccgctcg ctcaattggc acgttcacaa tcggcggcgc agcactgcgt
     1381 tctggtcggg agtgaacgtt gggatgatgc ataggtccta cagaaatggc tggtagtcat
     1441 tgaggtggca gacgcatgta cctccgttga gaagggtggt taggaaatgc caacattgga
     1501 cgcgcttgtc gttgccaatg tcttcgtgac aactgctcta ggcgttttgt accgttttcg
     1561 ccgtgacgtg ggtggcccgc aactcactcg gcccgggtcg aggctggcgc tgcttggcgt
     1621 cggtgccttt gtggggtggt acttggctgt tgccatcgcg cccaactcga gccattggtt
     1681 gctcaacgcc cctggggcgg cgattttgct ggccgtggaa cttgtgctct tcacgctcct
     1741 cgcgatggac accgtccggt ccgggcggag tgtgggcaag catgtgaggg cgccagaggg
     1801 gaacccagac gttcacaatc aaactctgga ctcccgaatg gatcggtgac gacacgccag
     1861 tgccaacgcc cgttcggcgc gatccctgac gcgttggccg cacaccgctg ccgacggccg
     1921 tccggcatcc gatctgcgct gccacgatga cggcatggcc ctggatgtcg agctgagcga
     1981 gacgcgcgac tttctcgcgt gccacgagcc cttcgatgcg ctcccgcagg gcgtgctcga
     2041 ccggctcccg gggcggatga gcgtgcagta cttccgccgc ggcacccgcc tcatcgagaa
     2101 ggggcgcgac aaccaccacc tgtacgtcct gcgctcgggc gccgccgacg tccacgaccc
     2161 tcagggcacg ctcgtcgacc gcggcggtga aggggcgtgc ttcggctcga tcacgctcac
     2221 cgagggcaac actcgacgtt cgaggtgacc gccctcgagg actgcctcgc gctcgtgctg
     2281 ccggccgagg acttccaccg gcaccgtgca gctttggctc tgtctgtgca gccggaggcg
     2341 cctcagggcc ccattgctcc gatcggcagc acgctgacgc cgtcgtccct ccggtacgcg
     2401 aagcccgtgg tggtgatgac tcccagtgcg acgggcgcac cggctttggc ctggtccacc
     2461 ttctcggaga atgacagcag tgacgcggcg gccttgtcgg cgtcttcggg attcatcttc
     2521 acctcgaagg cgccccaccg cccgtccggc agcgtgacca cgatgtccac ctcgttctgg
     2581 ttctggtctc gccagtggtg cacctcgccg ccaagcctct gggcgtacac gcgcatgtcg
     2641 cgcaccgcga gattctcgaa gaggtacccg gtcgcattca gatccctcag caactgctgg
     2701 gggccctgtc caagggccgc caccgccaat gatggatcga tgaagtagcg cgtgggcgag
     2761 gcctgcagcg gcgtcgcaga tcgcatgtgc ggtccccacg ccgacgagtt ctcgatcagt
     2821 cgcagtcggt ggagggaatc gaggtatccg tctacggtgg ccctcgccac cggcccatca
     2881 gccccgccga catccttggc cagtgcggtg acccttgccg acgtcgcaac gttccgtgcg
     2941 agggaggtga gcaggcggcg gatgttctgc ggatctcggc gacgcgtgcc tagttcctgg
     3001 atgtccacga ggacgatctg gttcaggtag tcccgcagcc aacgaccagc ctccgttgcg
     3061 gtggcgtcca ggagggccgg ccagccaccg atgcagatgc ggtcgatgat gtcgggcaag
     3121 gaggggccag aatcgcgacc agcaacgcgg cccccgtcca tcagtgaggc caacgaggcc
     3181 gtacccgatg agtggccgga ctcccagagc gacatgggcc gcatctggac aacggcgatc
     3241 cgacccgcgc cagaatgccg gttgacgtcg tcgcgcggtg tggcggagcc cgtcatgatg
     3301 aacagcccct tctcgggtga tcggtcgtcc acggctcgcc gcacgtgatc ccagagggcc
     3361 ggagcacgct gccactcgtc cagcaacacc ggacctggac tgtccagcag cacatctggg
     3421 gcgtaggtgg cagtgttgcg ggccgcttgg tcaacatcga gccggtgcat cgtcgcggcc
     3481 acctgagtgg cagtcgcggt cttaccgcat gccttggggc cttcgatgag cactgcgccg
     3541 atggacgtca gccgctcccg aagctcaagg tcaaccactc gcctgcggta accagccgtt
     3601 tccgtcatcg agtgcaccct tctgcttcca ttctccatcc ctctaacttt gcataaatcg
     3661 taacctaaat cttgcatatt tcataacgtt gacatggcgg aacccgcaat gtcaaagtcg
     3721 agtcgtctcg gcggccgtgg atctgggaga gcccttcgtg gtgtctgggg actgctgggc
     3781 ggaggtgacc gcacgctggt gtcgcaggcc gctcggcgcg ttcccccgcg cgttggtcgc
     3841 agagcggaac cggagggcgc ccggcatccg aggtgcgctg ccacgatgac gacatggccc
     3901 tcgatgtcga gctgaccgag acgcgcgact tcctcgcgcg tcacgagccc ttcgacgctc
     3961 tcccgcagag cgtcctcgac cgcctcccgg cacggatgac ggtgcagtac ttccgccgcg
     4021 gcagccgcct catcgagaag ggccgcgaca accaccacct gtacatcgtg cgctcgggcg
     4081 ccgccgacgt gcacgacccg cagggcaccc tggtcgaccg gggcggcgag ggcgcctgct
     4141 tcggctcgat caccctcacc caaggcaacc cgtcgacctt cgacgtcacg gcccgggagg
     4201 acactctcgc gctcgtcctg cccgctgacg acttccaccg cctgtgcgcc gaccagcccg
     4261 agttcgacgc cttcttcgac gcccagcgcg cgagccggat gagcggcgcg gtcgcctcgg
     4321 tgcagctgtc gagcaccggc ggggccatcc tcaagaccac cgtgcgtgac ctcatcgcgc
     4381 gcgagccgat cggtgtcgat gcccgcgtca gtgttcgtga cgcggcacgc ctgatgtcga
     4441 ccgagcgggt gtccgccctg ctcgtgaccc gcgacgggcg cctggccggc atcctcaccg
     4501 accgtgacct gcgcacccgc gtgctcgccg tcgacgtcga ccccgccacc cccgtcgccg
     4561 acgtcatgac cgccgacccc gtcaccggct ccgccgacgc cctcgccttc gaggtgctcc
     4621 tggagatggt cggccggcac atccaccacc tgccgatcct cgacgcccac gaccagcccg
     4681 tcgggctcgt cacgacgacc gacctgctgc gcctcgaaca gtccaacccc gtgtacctgg
     4741 cgggcgacat cgccaagcag gccgacgtcg cgggggtcgc ccgggccgcg agccggctgc
     4801 cgcaggtggt gctctcgctc gtcgagcagg acgcctcggc cgacgacatc ggccgcgtgg
     4861 tcacggcggt cggggatgcc gtggagcgtc gccttctcgc cctggccgag gccgagctcg
     4921 gcccgccacc cgtgccgtac tgctgggtgt cgctcggctc gcgggcacgc ctcgagcagg
     4981 cgctggccgc cgaccaggac acggcgatga tcatcgacga cgccatgctc cccgagcacg
     5041 agccgtggtt cgcggcgctg gcagagcggg tgaccgccga cctcgccgcc tgcggctacc
     5101 cccggtgcac cggcgacgtc atggccacca acccgcgctg gcgccaaccg cttgcgtcat
     5161 ggcgtcggca gttcacgtcc tggctcaccg aaccggtgcc cgacgccatc ctcggcgcga
     5221 gcatcttctt cgacatgcgc cccgtgcacg gtgacgcgac gctcgaggcc aggctccagc
     5281 gtcacgtgct cacggcatcc cccgacgcga aggtcttcct ggcgcacctc gcgaagcagg
     5341 ccaccgccaa cgagccgccc ctggggttct tccgcgggtt cgtgctcgag aaggcgggca
     5401 gccacgagaa caccctcgac atcaagcgcg gcggcatcgg cgcggtggtc gagctggccc
     5461 gggtgcacgc cctgtcactc ggcagtccgg cggtcaacac ccgggcccgg ctggacgcgg
     5521 cacacgcggc cggggtcctg ggcgacgacc gggccgctga cctgcgggac gcgttcgagt
     5581 tcatctcgta cgtccgcctg cgtcaccagg ccgcccaggt gcgggcgggg ctgccgccgg
     5641 acaaccacgt cgcccccgat gacctctcga gcttcgacaa gcgccacttg cgcgaagcgt
     5701 tcgccatcgt ccgccaggcg caaaccgctc tggcgcaccg gtacccgacc cactacatct
     5761 cgtgagcctc ttccggcgca cccccgacga acggcgccag cgggcgctgc gatcggccgc
     5821 gccggggccg ctgcgcgact acctcggcgt gccgttcccg gcgccgtcgg tgccgctgga
     5881 cgagttgccg ctgctcgccg tcgacatcga gaccaccggc ctcgacccac gcgtggaccg
     5941 cgtcctgtcg atcgggtggg tgcccgtcga cggcgggtcg gtggtgctcg gcggcgccgg
     6001 gcgggccgtc gtgcgcgacc aggtgggcgt ggccggggaa gccggggtgg ggtccagtgc
     6061 gaccatccac ggcctcaccg acgacgccct cgcgggtggg gaggtgatgg cggatgccgt
     6121 ggcgcgcctt ctcggcgccc tcaccggtcg tgtgctgttg gcacacttcg cgcggatcga
     6181 gacgaccttc ctctcggggg cgtgcgagcg gctctggggc gcgccgctgc cctgcgtcgt
     6241 cgtcgacacc ctggacctcg agcaccgggt cgtcacgggc cgggggcaga gggaccccga
     6301 gccgggggcg ctgcggttgt ggtcggcgcg ggagcggcac gggctgcccg tgtaccccgc
     6361 gcacgaggcg ctggtcgacg ccctggcctg cgccgagctc tacctcgcgc agcgcgccga
     6421 gctccgggcg gctcgtccgg aaacgacgct gaccctccgc caggtcatgg cgtgagggtc
     6481 aggtgatggt gctgcgcagg ctggggtgca gccggtgcac ggggtgagct gccggggggc
     6541 ggctcagaac atgcgagggc tgcggctctg cgcgagcagg tagagctcct gggactcgcg
     6601 aacgaggtcg cggtcgacgt gctcggtcca gccgcggccg tggtgacggc tcgtgcggag
     6661 ggcggaagcg agggagcggg ccttctgcaa ggagagagat gcggcgttca tgtcatccat
     6721 gatgcatcgc taaaccattc agcacaacgc gactttactt accagtaatg aagtatcgct
     6781 taagattgct tcatgcttga tccccaccgc ctcagggtct ttcgttccgt cgtcgccagt
     6841 ggctcggtcc aggccgcggc cgacaacctg gggttgacgt cgtcggcggt cagccagcac
     6901 gtcgcggctc tccagcgcga gaccgggctg accctgttcc accgggccgg gcgcggcatc
     6961 gtgccgaccg aggcggcgct ggccctcgtc gagcgcaccg atgaggtgat gagccggtgg
     7021 ggcgagctcg accaggccgt ggccgatctg cgcgagggcc gctcgggccg gttggcgatc
     7081 ggctacttcg cctccgcggg ggcggcgtgg atgccggcgc tcgtcaagcg gctgacccac
     7141 gagtaccccg acctcgtcct cgaactggtg ctgaccgagg ccgaggcgcg ggggtcgagc
     7201 accgggcgcg gccccgacat cgacctcgtc atcgacccgc cggacacccc ggtgcgatcg
     7261 gggtaccgcc gcatcgagct gacgaccgac ccgttcgtcg tcatcgttcc ggccggccac
     7321 ccgctcatcg ggcagggccc ggtgtcgctg tcagacctgc gccgcgagac ctgggtgagc
     7381 aacgactacc cgcgctcgat cgggcaccgc atggtggtgg cgcactgcaa cgcggtgggc
     7441 tttcgccccc gcttcaccgt ccaggcccag gaccactaca ccgccatccg gttcgtcgag
     7501 gccggcatcg gggtgtcggt gctgccgggg ctcgcggcgc gagacctccc ggcatccgtg
     7561 gtgcgcattc ctctggcccc gccggcaccg gtgcggcacc tggcggcgct cgtgcgtgat
     7621 gacggcaccc ccgacgaggc ggccgatcgg gcggtggccc tgctgaccga cctcatccac
     7681 cacccgagct cccgcctccg ctgaccccac accccgctca ggtggtggtg ggcacggggg
     7741 tggggggtgc gggggtgacg ctgcccaggg cgccggagtc gaggcgctcc gccaggcgaa
     7801 ccccctcgac gtcgaggtcg ggcaggatcc ggtcgagcca ccgcggcagg taccaggcac
     7861 ggtcgccgag caggtgcatg agagccggcg tgagcgtcat ccggatgagc acggcatcca
     7921 agagaacacc gaccgccagc gcgaacccga tcggccgcac catcgccagg tgcgagaacg
     7981 cgaacccgag gaagaccgag gtcatgatga tcgccgcggc cgtcacgacc ttggcgccgt
     8041 ggacgaagcc ggtgcgcacg gcggtgcgtg agtcctcgcc gtgggagcgg gcctcgtgca
     8101 tgccggagac gaggaacatc tggtagtcca tggccagccc gaacagcacg ccgatgatga
     8161 tgatcggcat caccgacagc agcggcccgg cctggtgcac cccgaacaga gcgccgagcc
     8221 acccccactg gtgcaccgcg acggtggcgc cgaaggccgc ggcgatcgac agcaggaagc
     8281 cgccggtggc gatgagcggc accacgatgg accggaagac gagcagcagg atgatgaccg
     8341 acagccccac gacgacggcg aggtaggccg gcagcgccgc ggcgagtcgc tccgagacct
     8401 cgatgttcgc cacggtctgg ccggtcaggc cgagcttgat gttctcgcgc cgctcgagct
     8461 gggatgccgt gaactcgacg cggtggaacg tctcggccgt cgcggggtcg gcgggcccgg
     8521 tggtgggcac gacctggaag gccagggtcg cctggtcggg gctgaccccg aacggcacga
     8581 cgctgcgcac gccctcgatg accttcaggc gggtggcgat catcgcctgc acgttggcca
     8641 gcgacgcctc atcggtcggt cgcagctcct cgggcagggt ggtcaccgcg acgatcgggc
     8701 cgttgatgcc ggggccgaac tgctctccga cggtggtgta cgcggtgtgc gccgtggtgc
     8761 cggttggttc gcgaccgcca tcgggcaggc cgagctggat cgagccgacc gggatggcca
     8821 gcaccccgag cacgcccacg acggcgagca ccgtgagcca cgggcgcagc gtcacgaggc
     8881 gcacgaacca cccgccgtgc tcctcgtccg ttccgtcgtc gggcgtcgtg cgagaggtcg
     8941 ggtcaccggg cgtggcgaac ccggcggcgc gccagccgcg cctggagatg acccgcgtgc
     9001 ccatgagtcg cagcagcgcc ggtgacaccg tcaccgcgac cagcacggtg acggccacgg
     9061 tgaaggcagc gacgaggccc atctcggcga ggatcggcag cccggacagc acgagggcag
     9121 tcagggcgat gacgacggtc agcccggcga agaccaccgc actgcccgca gtgccgaccg
     9181 cccgggagat cgactcgacg agctcctgcc cgtgcagcag tgacgtgcgg tgccgggtca
     9241 cgatgaacag cgcgtagtcg atgccgaccg cgaggccgag catgagcgcg agggcggggg
     9301 tcatctggtt gagctcggtc accgccgtga aggcgatggc gccgccgatg ccgacaccga
     9361 cgccgagcag cgcgacgagc aggggcaggc cggcggcgat gaggctgccg agcacggcca
     9421 cgagcacgag gagggcgacg gtgaggccga tgatctcgcc cgcaccgatg agcgcggtgt
     9481 cctgggtgat ctcgacgctg tagtcggcgg tgaccccggc ggcggccagg gcgtccttgg
     9541 cggtgtcggg gatcatcgcc cggttctcca ccggaacgct ctgcgcgtcg gtgtcgaact
     9601 gcacctgcgc gacggcgtag cggccgtcct cgctgaccag gcgggtgcct tcggtggctc
     9661 ggctgatcgc gacgccgtcc tcgtaggacg cccggccggc agccagctcc gcgatgccct
     9721 tctcgagctc ggcccgaccc ttctcgagct ctgcacggcc ctgggtcagc tgggcgcgca
     9781 gccccgggat gctcgggtcc tgcgggttgg tctcctcgag ctgcgccagc accgcctcgc
     9841 cggcagccag ctgcccttca ccctcggcca gcttggcctg cccctcgtcg atgcccttct
     9901 ggccggcgtc gagctgcacc ttcgcgtcgg cgagctgggc ctggccggcg tcgagctggt
     9961 cctgcgcctc gaacgggttg atgacccggc tgacgttcgg gacctcaccc catgccgcga
    10021 agacgtcctc gacggcggcc cgctgggcgg gggtgaaggc ggtgtccgag ctgagcacga
    10081 ccgtgccgaa cccgccggca gccttcggga tctcctcacc gagctgctgg cggaccttct
    10141 cgaagttggc tccggggatg gtgaacccgt tgcccagcgg ctgactgatc gtggctgcca
    10201 gggccccggc acccacgagg acgacgagcc agaggacgag cacgcgccgg gcgtgggtgg
    10261 cgcaccagcg gccgagtcgg tacagggcgg ttgacatcag tggtcctcag gggtgggggt
    10321 cggggtggcc gggtcgagcc cggtacgcag gtggtcaagg gctcgggcga ccgcggcgga
    10381 gagctcgccg gacggggcct tggcccagac gcggaaggcg gcctcggcgg cgccgagcat
    10441 cgtggcggca aaggtggcga cgagcaggtc gtcggtgccc ggtggcaggc ggcggcgcag
    10501 ccattcctcg agccagtcca gccaggtgtg ggcctcgacg aggatcagcc cgcgggcgtg
    10561 cggcgacgac tcacccgcgg ccgacagcac gccgatccgc tggagcagtt cgacgtcacc
    10621 ggggtcggtg atgacggcca cggcgctgtc gagcacgtcc tcgtcgtccg ggcgggcgtc
    10681 gaggcgttcg ccgacggagt cgaagaaggc ggcgacgctc gccgtgaann nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ctacgagcac cactccaacg agatcccgtg
    10861 gcgcgagtcg atcgccgacg tcgtgaccat ccgcgaggac gccgacggcg gggtgtgccg
    10921 cgacgacctg cgcgcccggc tggaggagta cgccgaccgg ccgctgaaga tcggctcgtt
    10981 cagcgcggca tccaacgtca ccggcatcgt ttcggacacc cacggggtgt cggcgctgct
    11041 gcacgagcac ggggcgctcg cgttctggga cttcgcggcg gccgcgccct acgtcgacat
    11101 cgagatgacc gcgccggtca gcggcgaccc ggccgcgtac aaggacgcga tcttcctgtc
    11161 gccgcacaag ttcatcgggg gcccgtcgac gcccggggtg ctcatcgccc gacgcgagct
    11221 gttcggcaac cgggtgccgg tcgtgcccgg cggcggcacg gtcgcctacg tcaaccccga
    11281 ggaccacgcg ttcgtcaccg acgccgcgca ccgcgaggag ggtggcaccc cggcgatcat
    11341 cgagtcggtg cgggccgggt tggtgttcca gctcaaggag gcggtcgggg tcgagacgat
    11401 ccgcgcccac gaggagcggc tgctctcgcg cgcggtcgag gcctggcggg ccgagccggc
    11461 gatcgagatc ctgggcaacc tcgacgcgcc gcggctgtcg atcgtctcgt tcgtcgtgcg
    11521 ggcgccctcg ggtcactggc tgcaccacaa cttcgtcgtc gccctgctca acgacctgtt
    11581 cggcatccag gcccgcggtg ggtgttcgtg cgcggggccc tacggccacc ggctgctcgg
    11641 catcgacatc gagcgctcgc acgagttcga gcgtgagatc accggcggct gcgagggcat
    11701 caaacccggc tgggtgcggg tgaacttcaa ctacttcatc tccgacaccg tcgcggacta
    11761 cgtcgtggat gccgtgcgtc tcgtcgcgcg cgacggctgg cggttgctgg gcgactacat
    11821 cttcgacccg gagaccgggc ggtggaggca ccgcgacggg gtcgtggagc cgcccatccg
    11881 gctgggggcg atctcgtacg ccgacgacgg gtcgatgcgg gtgccgcgca cggacaaccg
    11941 cggtggggag gagctgctgg cgcagcacct cgctgacggg gcggcgatcc tggcggcggc
    12001 gatcccgcct gatctgggtt cgcacgcgtc ggggctgtcg gatgacttcg agcacctgct
    12061 gtggttcgac ctgccgaccg gggctctcgg ctcgtgagct ctggtggttc tggtggttcc
    12121 gtgcagcccg tcggtgcgcc ggtgaccggg tggactgcgc gcccgctgcc ggcgggggtg
    12181 gtgctgacgg ggcgcacctg cctgctcgag ccgttgtcga gcgcgcacgt ggctggtctg
    12241 gccgaggcgt gggccggcac ggatgacgcg cactggacgt acctgccgat gccgcgcccg
    12301 cacaccccgg atgccgtggc gcacctcgtc gactccatgg tcggcgaccc cacgacggtg
    12361 gcctactgcg tgctcgtcga cgcgctggct gccggggtgc tgtcgttgat gcgcatcgac
    12421 ccagccaacg gggtggtgga gatcggggcg gtcatcctcg gcacgtcgct cgtgcggacc
    12481 ctggcgtcca ccgaggcgca gcgactgctc atggggcacg tgttcgacga cctcggctac
    12541 cgccgcctgg agtggaagtg cgacgcgctc aacgccccgt cgcggcgggc cgcgctgcgc
    12601 ctgggctaca ccttcgaggg agtcttccgc caggccctcg tcgtcaaggg gcgcaaccgg
    12661 gacacggcgt ggttctcggt gatcgacggc gagtggccgg cggtgcgcga gcggctcgac
    12721 gcgtggctgg acccggcgaa cttcgacgcg gccgggcgcc agcgc
//
LOCUS       DAOSOC010000057        12732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4925_length_12732_cov_7.691489, whole genome shotgun sequence.
ACCESSION   DAOSOC010000057 DAOSOC010000000
VERSION     DAOSOC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4925_length_12732_cov_7.691489"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PL014_06085"
     CDS             <1..406
                     /locus_tag="PL014_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOT56695.1"
                     /translation="TRHRHDLEMSTYEGPTGIVGVLADAATQVGIPSVSAWAAVPHYA
                     GHSPSPKATLALVSRLEELLDAPIEHADLDEDSRAWERGINELASSDSEVAEYVESLE
                     EAQDTADLPEASGDAIAKEFERYLRRRGTDED"
     gene            complement(413..1666)
                     /gene="mshC"
                     /locus_tag="PL014_06090"
     CDS             complement(413..1666)
                     /gene="mshC"
                     /locus_tag="PL014_06090"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HOT56696.1"
                     /translation="MKSWPSPFVPAVPGQGEPLALYDTQSRSVRPVQAGPVARVYVCG
                     ITPYDATHLGHAATYVTFDLVGRALRDAGHEVEYVQNVTDVDDPLLERAARDGRDWRE
                     LATSEIDLFREDMTALGVIPPMAYIGVVEAMPEIRTAVRSLIESGAAYGVPVPAAEGT
                     GQDVYLDLGQAPSFGSVSGWTEEQMDAVFGDRGGDPERVGKRQRFDPLLWRAAREGEP
                     SWDDEVLGAGRPGWHIECTAISLGHLGHPFDIKGGGSDLVFPHHEMSAVQALALTGGD
                     VFARHYVHQAMVGYQGEKMSKSKGNLVLVSRLRAQGVDPMAIRLVLLDQHYRTEWEYT
                     PALLEAAVTRLARWREALSVNEGADPASTIAAVRAAVADDLDTRAALGAVDAWADATL
                     AGQWDDAAAPGVLARSIDAILGIRL"
     gene            complement(1690..2499)
                     /locus_tag="PL014_06095"
     CDS             complement(1690..2499)
                     /locus_tag="PL014_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HOT56697.1"
                     /translation="MPTATDAELVDLLTAGTLTPVGRLVNSSNGALLCSVQGPDCDAE
                     PLLAIHKPESSERPLWDYPDGTLVARERAAYLVSTAGGFGVVPPTVLREGPFGPGSVQ
                     LWVGPLEGEREVLVDVTAAEAVPPGWLVVLEGETPEGEPVVVSHSPEPALRTVAVLDA
                     VLNNSDRKGSHLMREGSIVRGCDHGVSLGVEPKLRTVLWGWAGDEILDADLARLDALG
                     QALAGSLATDLAALLTVVEVAALRDRVSTLLATRRHPLPTPGWPAIPWPAL"
     gene            complement(2477..3037)
                     /locus_tag="PL014_06100"
     CDS             complement(2477..3037)
                     /locus_tag="PL014_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HOT56698.1"
                     /translation="MPTTVFDPPERFVAGTVGPPGGRTFFLQARGEGRLYSVSLEKVQ
                     VTVLADRINDVLDEQADGLATEPFGDGDVDPLDTPIEDEFRVDTLSLAWDPNRRVIII
                     ECHDQDPAELEGLDTTELEELSGLAPLVDQQVTSLRVVLTPVQARAFARRCARVVSAG
                     RPACPFCGQPLDPTGHICPRANGYRR"
     gene            complement(3048..3779)
                     /locus_tag="PL014_06105"
     CDS             complement(3048..3779)
                     /locus_tag="PL014_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56699.1"
                     /translation="MSVPVLDLRGVAYSYPGGHQALFGVDLHVHRGERVALLGPNGAG
                     KTTLVLHLNGILVPGAGTVTVSGLPVSHENLLEVRRRVGIVFQDPDDQLFMPTVRDDV
                     AFGPANLGLRGPELESRVVEALTLVGVADLADRAPHHLSFGQRRRVAIATVLAMRPEI
                     LVLDEPSSNLDPASRRELAEILRSLDVTVLMVTHDLPYAVELCERSVVLSEGAVVADG
                     ATRTVLADADLMARHRLELPTGFSI"
     gene            complement(3776..4537)
                     /gene="cbiQ"
                     /locus_tag="PL014_06110"
     CDS             complement(3776..4537)
                     /gene="cbiQ"
                     /locus_tag="PL014_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOT56700.1"
                     /translation="MGGGHSRHLHVAGHTPLHRMPAHAKLVGLLTFVLAVVSLPRGAH
                     WPLLGMLAVAVGVLASTRVPFSLLLPRMVVEVPFVVFAMVMPFVALGPRVQLGPLEVS
                     GPGLEAAVTLLLKGTTGVLAAIAFAVTTRPRDLVLALQHLRCPDQLVLIVAFMVRYTD
                     VVIDQMRRMRVARESRGFVARSPRAWPALASSSGALFIRSYERGERVHLAMLSRGWSG
                     RHPATVPLAATPAQWALALTPATIALITSVLVWAR"
     gene            complement(4539..4889)
                     /locus_tag="PL014_06115"
     CDS             complement(4539..4889)
                     /locus_tag="PL014_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HOT56701.1"
                     /translation="MSTADSSTDTRPGRAPRSARAVVVVGVVISLLLAGLVSSYASSH
                     PDGLDYVGQSLGFGESARDSAVAGSPLADYAVSGVDDARLSGGLAGVVGVLVTGVLMA
                     GVLLLVRRSSRRRR"
     gene            complement(4886..5572)
                     /locus_tag="PL014_06120"
     CDS             complement(4886..5572)
                     /locus_tag="PL014_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOT56702.1"
                     /translation="MHVPDGFLDAPTSVATAAVAVVAVGASLRGARRELDDRTAPLAG
                     LVAVFIFAAQMLNFPVGVGTSGHLIGATLAAVLVGPWTATLCMTVVLTVQALLFADGG
                     VSALGTNIVLMGVVAVWVGYGVFRLVLALLPRILASVSAAAGVGALLSVPASALAFVG
                     LYAVGGATPLPLGAMAAAMGGWHLVIGLGEAAITLLAVSSILAARPDLVHGAAGRLPA
                     LELRTTAVSA"
     gene            complement(5647..6390)
                     /locus_tag="PL014_06125"
     CDS             complement(5647..6390)
                     /locus_tag="PL014_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HOT56703.1"
                     /translation="MAIVLLLRHGHSTANEQGILAGWTPGVGLTEQGRRQASDVARRL
                     SSVTLARAVASPLQRCAETAALALAGADTTPETDDRLGECHYGAWTGLHLAEAAKDDL
                     WRVVQDDPAAATFPPHDVFRAESLLTMAQRVVGAVRELDAEIEAAHGSDAVWLAVSHG
                     DPIKALVAEAVGAGLAGLQRVRVDPGSLSVVQITPTRMVLLASNTTQGDLAPLVSVPR
                     HTPAGDGAVGGGDGGSRPEGPEQNGQHGT"
     gene            6437..>6807
                     /locus_tag="PL014_06130"
     CDS             6437..>6807
                     /locus_tag="PL014_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOT56704.1"
                     /translation="MIVDQAIYVGGARRPCMDLSDELATLREEGAPGDFLWVGLKDPT
                     QAEFDEVNDELGLHSLAVEDAIKGRQRAKVELYDNTVFVVLKPLRYLEESSDIETGEL
                     MCFIGDHFLVTVRRGEASPLAG"
     assembly_gap    6808..6907
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6908..7299
                     /locus_tag="PL014_06135"
     CDS             <6908..7299
                     /locus_tag="PL014_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOT56705.1"
                     /translation="HSLHADAASRVTEAELRLLFRDVSDHLQAVIDHADSYDRLLTDV
                     LSAHLAQVSVQQNNDMRRISAWVAMAAVPTMIAGIYGMNFDFMPELGASVRIGDGEFR
                     YGYFVVLGVMASVVATLYRAFKRSGWL"
     gene            complement(7308..8573)
                     /locus_tag="PL014_06140"
     CDS             complement(7308..8573)
                     /locus_tag="PL014_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HOT56706.1"
                     /translation="MSLAAPAFDVVMLGGDIGVYSLARAFHEAFGVRSTVISRIATGP
                     VDSAIIDNVLLGAEASNEDHIAELLRRGRERTAAEAALGREATPVILLANTDGYSRLF
                     AAHRAELDAFYRISLVDVATLDQISDKGRFAEACTEIGVPTPITHIEDFSGAGEPGWA
                     PSPMTIPFPVVAKAAASSVHELVDYPGKKKVNFIQTPAELDDLWTRLKDAGFRDRFIV
                     QELVPGDDTWMRSVTAYRDHRGSVTLMCTAQVLLEEHTPSALGNPCAMITTPFPEAMA
                     HARSLLDHLDYRGFANFDAKVDPRDGSFRFLEVNPRIGRNNYYVTGAGANVARFIVAD
                     LIEDRAAEPVTVTNEIVYSVVPKKLLLRYITDETLRRHVKQVMRKGIVHPLRYRAEGP
                     KRRAYVLAAKANHVKKFLTYYPRPTESGF"
     gene            complement(8614..9708)
                     /locus_tag="PL014_06145"
     CDS             complement(8614..9708)
                     /locus_tag="PL014_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT56707.1"
                     /translation="MDLAITCGYAGMGLGTDTLELVQVAESLGYRQAWVAEAYGSDAP
                     TVLAWLAAQTSTIGLGAGVMQIPARTPAMTAMTAATLDTLSQGRFHLGLGVSGPQVSE
                     GWHGVRFTSPLQRTREYVDVVRMALRREQVTYAGEHVTLPLPDGPGKALRLTIRPPRP
                     DLPIYLAAVGPRNLRLAGEVADGWLGIFFSPEHSADMLDAVRAGREVRAASEGAPAGD
                     VMAGFDVVVTVPVVVTDDLEAGRAAVADYTALYIGGMGSRKQNFYNALARRMGFDDAA
                     DEIQDLYLAGRPRDAASAVPFELADATALIGSPERIAERIGRYADAGVSTLSVAPFAA
                     STAQRAEVLRTVKTALGASARGGGTSRVGR"
     gene            9752..10696
                     /locus_tag="PL014_06150"
     CDS             9752..10696
                     /locus_tag="PL014_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT56708.1"
                     /translation="MLTRRLGASGLAVSRLGLGTMAWGTRTSPEDARDLLGLFRAAGG
                     TLIDTAHGYAGGASEELLGDLLQGPDRDEVVIVTKSGISRATGTRVVDCSRRAMMHQL
                     ETSLRRLRTDHVDVWLAHSWDEHTPIEETVSALLWAVESGRARYVGVSNHVGWQAART
                     MSLLTASGVPLVANSVEYSLVARAPEHELVDAGTALGFGLLPWSPLGRGVLAGRYRTT
                     VAADSRLASEEFGRFASKHVGEHTRGVVEAVRTAAAGLGVAPAVVALAWVRDRQAVAA
                     PIIGPRTPTQLAALLESEDVDLPEQIVAALDEVSIEHT"
     gene            complement(10713..11006)
                     /locus_tag="PL014_06155"
     CDS             complement(10713..11006)
                     /locus_tag="PL014_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HOT56709.1"
                     /translation="MSADPRAALNTLVAALERHLEASSARRGEDDPAVIAAYDDLADA
                     FEDYDGALFDAYGEMTPLDIYVDDDAHVDEDGDDAHLYSGLDDDIDLDDDVER"
     gene            11044..11241
                     /locus_tag="PL014_06160"
     CDS             11044..11241
                     /locus_tag="PL014_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HOT56710.1"
                     /translation="MVEYEYLTLTFGRETARGDIRRLLTEHAEYGHWELDRTRIYVGG
                     RTRTRLRRRIIRARRAVSTSR"
     gene            complement(11225..12550)
                     /locus_tag="PL014_06165"
     CDS             complement(11225..12550)
                     /locus_tag="PL014_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT56711.1"
                     /translation="MRETTTAAQDEVVQICQDLIRIDTSNFGDGSGPGEAEAAAYVVQ
                     SLREVGLEPQTFESDPGRVSVVVRIPGADRERGALCIHGHLDVVPANAADWSVDPFSG
                     ELRDGCVWGRGAVDMKDMDAMILACVRDLARTGTVPPRDLVIVFFADEEAGGRKGSHF
                     LVEQHPEVFDGVTEAISEVGGYSVTVTDRHGDERRTYLLQTAEKGICWLRLTAHGRAG
                     HGSVPNDENAIVRLAEAISRIAAHTWPREFIPSVRTLLEGLGELTGTSWSDEDLEDLL
                     DHLRGAQGFVRGTLQDTSNVTMLDAGYKHNVIPQHASANVDCRFLPGHEEQLLATITE
                     LAGEHVSVEVLHRDIALDAPFEGDLVEAMVAALRTEDPDAVVLPYCLSGGTDNKALSL
                     LGITGYGFAPLRLPADLDFAPMFHGVDERVPVDALQFGARVLGRLIATC"
BASE COUNT         1883 a         4328 c         4674 g         1747 t
ORIGIN      
        1 cacccgccac cgccacgacc tcgagatgag cacgtacgag gggccgaccg gcattgtcgg
       61 cgtgctcgcg gatgccgcga cccaggtcgg catcccgtcg gtgtcggcgt gggccgcggt
      121 cccgcactac gccggccact ccccctcacc caaggccacg ctcgccctgg tcagccgcct
      181 cgaggaactg ctcgacgccc ccatcgagca cgccgacctc gacgaggact cacgcgcctg
      241 ggaacgtggc atcaacgagc tcgcgtcgtc ggacagcgag gtcgccgagt acgtcgagag
      301 cctcgaggag gcgcaggaca ccgccgacct gcccgaggcc agcggtgacg ccattgccaa
      361 ggagttcgag cgctacctgc gccgacgggg caccgacgag gactgagtcc cctcagaggc
      421 ggatgccgag gatcgcgtcg atgctgcgcg cgagcacccc gggggctgcg gcgtcgtccc
      481 actggccggc cagggtggcg tcggcccacg catcgaccgc acccagggct gcccgcgtgt
      541 cgaggtcgtc ggccaccgct gcgcgaaccg ccgcgatcgt ggatgccggg tcggcacctt
      601 cgttgaccga gagcgcctca cgccaccggg ccaaccgcgt cacggcggcc tcgagcaggg
      661 ccggggtgta ctcccactcg gtgcggtagt gctggtcaag cagcacgagg cggatggcca
      721 tcgggtcgac gccctgtgca cgcagccgcg agacgagcac gaggttgccc ttggacttgc
      781 tcatcttctc gccctggtag cccaccatcg cctggtggac gtagtggcgg gcgaacacgt
      841 cgccaccggt gagcgccagt gcctgcacgg cgctcatctc gtggtgcggg aacacgaggt
      901 cgctgccgcc gcccttgatg tcgaacgggt gtccgaggtg cccgagcgag atcgccgtgc
      961 actcgatgtg ccagccgggg cggcctgcgc ccagcacctc gtcgtcccag gagggctcac
     1021 cctcgcgggc cgcgcgccag agcagcgggt cgaagcgctg gcgcttgccg acgcgctcgg
     1081 ggtcgccgcc gcggtcgccg aacacggcat ccatctgctc ctcggtccac cccgagacgc
     1141 tgccgaatga cggggcctgc ccgaggtcga ggtagacgtc ctgcccggtg ccctcggccg
     1201 ccgggaccgg gaccccgtag gcggcgccgc tctcgatgag ggagcgcacg gcggtcctga
     1261 tctcgggcat ggcctcgacg acaccgatgt aggccatcgg ggggatgaca cccagcgcgg
     1321 tcatgtcctc ccggaagagg tcgatctcgg aggtcgcgag ctcgcgccag tcgcgcccgt
     1381 cgcgagcggc ccgctcgagc agcgggtcgt cgacgtcggt gacgttctgc acgtactcga
     1441 cctcgtgccc ggcgtcgcgc agggcacggc cgacgaggtc gaaggtcacg tacgtcgcgg
     1501 cgtgcccgag gtgggtcgcg tcgtacgggg tgatgccaca gacgtagacg cgggccaccg
     1561 ggccggcctg caccggtcgc accgagcgac tctgggtgtc gtagagggcg agaggctcac
     1621 cctgaccggg aacggcggga acgaacggcg agggccacga cttcaccggg caagactacg
     1681 tgccgctggc tacagcgcag gccacgggat cgccggccac cccggagtcg gcagcggatg
     1741 ccgtcgcgtg gccagcaggg tgctgaccct gtccctcagt gcggcaactt cgacgaccgt
     1801 gagcagcgcc gccagatcgg tggccagtga gcctgccagg gcctgcccga gggcgtcgag
     1861 gcgcgcgagg tcggcgtcga ggatctcgtc gccggcccac ccccacagga ccgtgcgcag
     1921 cttgggttcc accccgaggc tgacaccgtg gtcgcacccg cgcacgatcg acccttcccg
     1981 catcaggtgg ctgcccttgc ggtcggagtt gttgagcacc gcatcgagca cggccacggt
     2041 gcgcagcgcc ggctccgggc tgtgcgacac gacgaccggt tcgccctccg gggtctcgcc
     2101 ctcgagcacg acgagccaac ccgggggtac cgcctcggct gcggtgacgt cgacgagcac
     2161 ttctcgctcc ccctccagcg gccccaccca cagctggacc gagccggggc cgaacgggcc
     2221 ctcgcgcagc accgtcggcg ggacgacccc gaaacctccg gcggtgctga cgaggtaggc
     2281 agcccgttca cgggcgacga gcgtgccgtc cgggtagtcc cacaggggcc gctcgctgga
     2341 ctccggcttg tggatggcga gcagcggttc ggcgtcgcag tccgggccct gcaccgagca
     2401 gagcagggca ccgttcgagg agttgacgag ccggccgacg ggagtgagcg tgcctgccgt
     2461 cagcaggtcg acgagctcag cgtcggtagc cgttggcacg aggacagatg tgaccggtcg
     2521 ggtccagcgg ctgaccgcag aacgggcagg ccgggcggcc ggcgctcacc acgcgggcgc
     2581 accggcgggc aaaggcacga gcctgcaccg gcgtgaggac gacccgcagg ctcgtgacct
     2641 gctggtccac gagcggcgcg agaccgctca gctcctcgag ctcggtggtg tcgagaccct
     2701 cgagctcggc agggtcctgg tcgtggcact cgatgatgat gacgcggcgg ttggggtccc
     2761 aggccaggga cagggtgtcg acgcggaact cgtcctcgat cggggtgtcc agcgggtcga
     2821 cgtcaccgtc gccgaagggt tcggtggcca ggccgtcggc ctgttcgtcg aggacgtcgt
     2881 tgatccggtc ggcgagcacg gtcacctgga ccttctccag ggacaccgag tagagccggc
     2941 cctcccccct ggcctggagg aagaaggtgc ggccaccggg tggccccacg gtcccggcga
     3001 cgaagcgctc gggtgggtcg aagacggtgg tgggcatgaa caccacccta gatcgagaag
     3061 cccgtcggca gctcgaggcg gtgtcgagcc atgaggtcgg cgtctgcgag gacggtgcgg
     3121 gtggcgccgt cggcgacgac cgcgccctcg gagagcacga cggagcgttc acacagctca
     3181 acggcatacg gaaggtcgtg ggtgaccatg agcacggtga cgtccagcga acgcaggatc
     3241 tcggcgagct ccctgcggga agcggggtcg aggttggacg acggttcgtc gaggacgagg
     3301 atctcgggtc gcatcgccag cacggtggcg atcgcgacgc ggcggcgttg cccgaaggag
     3361 aggtggtgcg gggcccggtc ggcgaggtcc gcgaccccca cgagggtgag cgcctcgacg
     3421 acacgtgact cgagctcggg gccgcgcagc ccgaggttgg ccgggccgaa ggcgacgtcg
     3481 tcgcgcaccg tgggcatgaa cagctggtcg tccgggtcct ggaagacgat gccgacgcgt
     3541 cgacggacct cgaggaggtt ctcgtgggac accggcagcc ccgaaaccgt gaccgtcccg
     3601 gcaccgggga cgaggatgcc gttgaggtgc aggaccaggg tggtcttgcc ggcaccgttg
     3661 ggaccgagca gggccacgcg ttcgccgcgg tgcacgtgca ggtcgacccc gaacagtgcc
     3721 tgatggcccc ccgggtagga gtaggcgacc ccgcgcaggt cgaggacggg cacgctcatc
     3781 gcgcccacac cagcacggag gtgatgagcg cgatcgtggc cggggtgagg gccagggccc
     3841 actgcgctgg cgtggcggcg aggggcacgg tggcggggtg ccgtccggac cacccgcggc
     3901 tcagcatcgc caggtggacc cgttcgccgc gctcgtagct gcggatgaac agggcacccg
     3961 agctcgaggc gagcgcgggc caggcccggg gtgagcgcgc cacgaagcct cgggactcgc
     4021 gggccaccct catgcggcgc atctggtcga tgacgacatc ggtgtagcga accatgaacg
     4081 ccacgatcag cacgagctgg tccgggcagc ggaggtgctg cagggccagc acgaggtcac
     4141 gagggcgggt cgtcacggcg aacgcgatcg ccgcgaggac acccgtggtg cccttgagca
     4201 gcagggtcac ggccgcttcg aggccgggcc cggacacctc cagcggcccc agctgcaccc
     4261 gcgggccgag cgcgacgaac ggcatcacca tggcgaagac gacgaacggg acctcgacca
     4321 ccatccgagg aagcaggagc gagaacggga cccgggtcga ggccagcacc ccgacggcca
     4381 ccgcgagcat gccgagcagc ggccagtgcg caccgcgcgg cagggagacg acggcaagga
     4441 cgaacgtgag cagaccgacc agcttggcgt gcgccggcat ccggtgcagt ggtgtgtgcc
     4501 cggcgacgtg caggtgtcgc gagtgccccc cgcccacctc agcgacggcg gcgcgacgag
     4561 cgccggacca gcagcaggac gcctgccatg agcacaccgg tcacgagcac gccgaccact
     4621 ccggcgaggc cacccgagag ccgcgcgtcg tcgaccccgg agacggcgta gtccgcgagc
     4681 ggcgacccgg cgacggcgga gtcacgggcg gactcgccga aaccgaggct ctgccccacg
     4741 taatcgaggc cgtcggggtg gctggaggcg taggacgaga cgagcccggc gaggagcagg
     4801 ctgatgacga ctccgacgac gaccacggcc cgggcggacc gcggcgcgcg cccggggcgg
     4861 gtgtcggtgg acgagtctgc ggtactcatg cgctcaccgc cgtcgtcctg agctcgagcg
     4921 ccggcagccg gcccgcggcg ccgtgcacga ggtcgggcct cgcggccagg atgctgctca
     4981 ccgccaacag cgtgatggcg gcctcaccca gaccgatgac gaggtgccag ccacccatcg
     5041 cggcggccat cgcgccgagc ggcaggggtg tggccccacc gacggcgtac aggccgacga
     5101 acgccagcgc tgacgcgggc acgctgagca gggcgccgac accggccgcg gcgctgaccg
     5161 aggcgaggat tcgtggcagc agcgccagga cgagacggaa gaccccgtag cccacccaga
     5221 ccgcgacgac ccccatgagc acgatgttcg tgcccagggc gctcactccc ccgtcggcga
     5281 agagcagcgc ctgcacggtg aggacgaccg tcatgcacag cgtcgccgtc cacgggccga
     5341 cgaggaccgc ggcgagggtc gccccgatga ggtggccgga ggtgccgacc cccacgggga
     5401 agttgagcat ctgcgcggcg aagatgaaca ccgcgaccag gccggccagc ggggcggtgc
     5461 ggtcgtcgag ctcacggcgg gcaccgcgca gcgacgcgcc gacggcgacg acggccaccg
     5521 cagcggtggc caccgaggtc ggcgcgtcga ggaatccgtc ggggacgtgc atgcaggctc
     5581 ctgcccgggc agttgttgcg acttgtttgc aacaagccta acgaccggcg gggccgccgg
     5641 ccaccatcag gtgccgtgct ggccgttctg ctcggggccc tcgggcctcg acccaccgtc
     5701 gccgccaccc acagcaccgt ctccggccgg ggtgtgccgc ggcacggaca cgaggggggc
     5761 gaggtcgccc tgagtggtgt tgctcgccag caggaccatc cgcgtgggcg tgatctgcac
     5821 gacgctcaga ctgcccgggt cgacgcgcac gcgctgcagg ccggccagcc cggcgcccac
     5881 ggcctcggcg acgagtgcct tgatggggtc accgtggctc acggccagcc acacggcatc
     5941 cgagccgtgg gccgcctcga tctcggcatc gagctcccgc acggcaccca cgacgcgctg
     6001 cgccatggtg agcagcgact cggcccggaa gacgtcgtgc ggtgggaagg tcgcggcggc
     6061 ggggtcgtcc tgaaccaccc gccagaggtc gtccttggcc gcctcggcca ggtggaggcc
     6121 ggtccaggct ccgtagtggc actcgccgag gcggtcgtcg gtctcgggtg tggtgtcggc
     6181 acccgcaagg gccagggcgg cggtctcggc acagcgttgc agcggactgg ccacggcccg
     6241 cgcgagcgtc accgaggaca gccggcgggc gacgtccgag gcctggcgcc ggccctgctc
     6301 ggtgaggccg acaccgggag tccagcctgc gaggatcccc tgctcgttgg cggtggagtg
     6361 cccgtggcgc aggagcagga cgatggccac gggatgactg tatgccggcg cgcccccatc
     6421 ggacacaatg cgaggcgtga tcgtggacca ggccatctac gtcggcggtg cccggcggcc
     6481 ctgcatggac ctcagtgacg agctggcgac ccttcgcgag gagggcgcgc cgggtgactt
     6541 cctgtgggtg ggcctcaagg accccaccca ggccgagttc gacgaggtca acgacgagct
     6601 gggcctgcac tcgctggccg tcgaggacgc catcaagggg cgccagcggg cgaaggtcga
     6661 gctgtacgac aacaccgtct tcgtcgtcct gaagccgctg cggtacctcg aggagtcctc
     6721 ggacatcgag accggtgagc tgatgtgctt catcggggac cactttctcg tcaccgtgcg
     6781 ccggggcgag gccagcccgc tcgccggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnntgc actcactgca cgcggatgcc gcgtccaggg tcaccgaggc ggagctgcgg
     6961 ttgttgttcc gcgacgtctc ggaccacctg caggcggtca tcgaccacgc ggactcctac
     7021 gaccgcctcc tcaccgacgt gctgagcgcg cacctggcgc aggtgagcgt gcagcagaac
     7081 aacgacatgc ggcgcatctc ggcgtgggtg gccatggccg ccgtccccac catgatcgcc
     7141 ggcatctacg gcatgaactt cgacttcatg cccgagctcg gggccagcgt ccgcatcggt
     7201 gacggggagt tccgctacgg ctacttcgtg gtgctcgggg tcatggcgtc agtggtcgcc
     7261 accctctacc gggcgttcaa gcgcagtggc tggctgtaga ccaggcctca gaagccgctc
     7321 tcggtgggcc gcgggtagta cgtgaggaac ttcttcacgt ggttggcctt cgcggccagg
     7381 acgtaggcgc ggcgcttggg cccctccgcc cggtagcgca gcgggtggac gatgcccttg
     7441 cgcatcacct gcttgacgtg gcgtcggagc gtctcgtcgg tgatgtagcg cagcaggagc
     7501 ttcttgggca ccacggagta cacgatctcg ttggtgacgg tcaccggttc ggcggcgcgg
     7561 tcctcgatca ggtcggccac gatgaatcgg gccacgttgg cgccggcgcc ggtcacgtag
     7621 tagttgttcc gcccgatgcg ggggttgacc tcgaggaagc ggaaggagcc gtcgcggggg
     7681 tcgaccttgg cgtcgaagtt cgcgaagccg cggtagtcca ggtggtccag gaggctgcgc
     7741 gcgtgcgcca tcgcctcggg gaacggggtc gtgatcatcg cgcagggatt gcccagggcc
     7801 gacggggtgt gttcctcgag gagcacctgg gcggtgcaca tgagcgtgac cgatccgcgg
     7861 tggtcgcggt acgcggtgac cgaccgcatc cacgtgtcgt caccgggcac gagctcctgg
     7921 acgatgaagc gatcgcggaa cccggcatcc ttcagtcggg tccacaggtc gtccagctcg
     7981 gccggggtct ggatgaagtt gaccttcttc ttgcccgggt agtcgacgag ctcgtggacg
     8041 ctcgatgccg cggccttggc gacgacggga aacgggatgg tcatcggcga cggggcccag
     8101 cccggctccc cggccccgga gaagtcctcg atgtgggtga tcggggtggg gaccccgatc
     8161 tcggtgcagg cctcggcgaa gcggcccttg tcggagatct ggtcgagggt ggcgacgtcg
     8221 accagggaga tgcggtagaa cgcatcgagc tcggcccggt gggccgcgaa caggcgcgag
     8281 tagccgtcgg tgttcgcgag caggatgacc ggagtcgcct cgcgaccgag cgcagcctcg
     8341 gctgcggtcc gctcccggcc ccggcgcagc agctcggcga tgtggtcctc gttgctggcc
     8401 tcggcaccga gcagcacgtt gtcgatgatc gccgagtcga ccgggccggt cgcgatcctc
     8461 gagatgaccg tcgaacgcac cccgaatgcc tcgtggaagg ctcgagccag cgagtagacc
     8521 ccgatgtccc ctccgagcat gacgacgtcg aaggccggtg cggcgaggga cattgatctc
     8581 cttgaacggt gtgcggggac ggacctgccg atcctatcgg ccgacgcggg acgtcccgcc
     8641 gccgcgcgcg ctggcgccca acgccgtctt gaccgtcctc aggacctcgg ctcgctgcgc
     8701 ggtgctggcc gcgaacgggg cgacactcag cgtgctcacc ccggcgtccg cgtaccgacc
     8761 gatgcgctcg gcgatgcgtt cgggtgagcc gatgagcgcc gtggcgtcgg cgagctcgaa
     8821 gggaacggcc gacgcggcat cccggggacg accggcgagg tagaggtcct ggatctcgtc
     8881 cgccgcgtcg tcgaagccca tcctgcgagc cagcgcgttg tagaagttct gcttccggct
     8941 gcccatgccg ccgatgtaga gcgcggtgta gtcggccacg gcggcgcgcc ctgcctcgag
     9001 gtcatcggtg acgacgacgg gcacggtcac gacgacgtcg aaaccggcca tcacgtcgcc
     9061 cgctggcgct ccctcggatg ccgcgcggac ctcccgtccc gcgcggacgg catccagcat
     9121 gtcggcgctg tgttccggcg agaagaagat gccgagccag ccgtcggcga cctcaccagc
     9181 gaggcggagg ttgcgcggtc cgacggcggc gaggtagatc ggcaggtcgg gccgcggtgg
     9241 cctgatcgtc aggcgcaggg cctttccagg cccgtccggc aacggcagag tcacgtgctc
     9301 accggcgtag gtgacctgtt cgcggcgtag ggccatccgc acgacgtcga cgtactcgcg
     9361 ggtgcgctgc agcggtgagg tgaagcgcac cccgtgccag ccctcgctca cctgcggccc
     9421 ggagaccccc agccccaggt ggaaccggcc ctgcgagagg gtgtcgaggg tggcggccgt
     9481 catcgcggtc atcgccgggg tgcgcgccgg gatctgcatg acgccggcac cgagaccgat
     9541 ggtgctcgtc tgcgcggcca accaggcgag cacggtcggt gcgtcggacc cgtacgcctc
     9601 ggccacccac gcctgcctgt accccaggct ctcggccacc tggacgagct cgagggtgtc
     9661 ggtgccaagt cccatgccgg cgtagccgca ggtgatcgcg aggtccatgg cctccacggt
     9721 agcgagggcc tcgggcgata aggtgcccgc catgctcacc cgccgcctcg gtgcctccgg
     9781 tctggccgtc tcccggctcg gcctcggaac gatggcgtgg ggcacccgca cctcccccga
     9841 ggacgctcgc gacctgctcg gccttttccg ggcagcgggc gggacgctca tcgacacggc
     9901 ccacggctac gcgggagggg cctccgagga gctgctcggg gatctgctcc agggcccgga
     9961 ccgcgacgag gtcgtgatcg tcacgaagtc ggggatcagc cgggcgacgg gcacgcgggt
    10021 cgtggactgc tcccggcggg ccatgatgca ccagctcgag acctcgcttc gccggctgcg
    10081 caccgaccac gtcgacgtgt ggctcgccca ctcctgggac gagcacaccc cgatcgagga
    10141 gaccgtcagc gccctgctgt gggccgtgga gtccgggcgg gcgcgctacg tcggggtgtc
    10201 gaaccacgtg ggctggcagg cggcccgcac gatgtccctg ctcacggcat ccggggtgcc
    10261 gctcgtcgcc aactccgtcg agtacagcct cgtcgcgagg gcccccgagc acgaactcgt
    10321 ggatgcgggc accgcgctgg ggttcgggct cctcccgtgg tcaccgctcg ggcgcggggt
    10381 gctggccggt cgctaccgca ccaccgtggc cgccgactcg cgcctggcct ccgaggagtt
    10441 cgggcggttc gcctccaagc acgtgggcga gcacacccgc ggtgtcgtcg aggccgtgcg
    10501 caccgcggcc gccgggctcg gggtcgcgcc agccgtcgtc gcgctcgcct gggtgcggga
    10561 tcgtcaggcg gtggctgcgc cgatcatcgg gccgcgcacc cccacccagc tcgcggcgct
    10621 gctggagtcg gaggacgtcg accttccgga gcagatcgtg gcggcgctcg acgaggtgtc
    10681 gatcgagcac acctgagtcg gatgccggtc gctcaccgct cgacgtcgtc gtccaggtcg
    10741 atgtcgtcgt ccagccccga gtacaggtgg gcgtcgtcgc cgtcctcgtc gacgtgcgcg
    10801 tcgtcgtcga cgtagatgtc gagcggtgtc atctcgccgt acgcgtcgaa cagcgccccg
    10861 tcgtagtcct cgaaggcgtc ggcgaggtcg tcgtaggcgg cgatgacagc cgggtcgtcc
    10921 tccccgcgac gagcgctgct tgcctccagg tgcctttcga gggcggcgac gagggtgttc
    10981 agggcggcac gcgggtcagc gctcatggtg tcgacggtac tcgccgcttg gcacaatgtg
    11041 gtgatggtcg agtacgagta cctcaccctg acgttcgggc gggagaccgc gcggggtgac
    11101 atccgccgtc tgctgaccga gcacgccgag tacgggcact gggagctcga ccgcaccagg
    11161 atctacgtcg gcggtcggac ccgcaccagg ttgcgccgcc ggatcatccg ggcacggcgg
    11221 gccgtcagca cgtcgcgatg aggcgcccga ggaccctggc tccgaactgg agcgcgtcga
    11281 ccgggacacg ctcgtcgacc ccgtggaaca tgggggcgaa gtcgagatcg gccggcaggc
    11341 gcagtggggc gaacccgtag ccggtgatgc ccagcagcga gagtgccttg ttgtcggtgc
    11401 cgccggacag gcagtacggc aacacgacgg catccgggtc ctcggtgcgc agcgcggcga
    11461 ccatcgcctc gacgaggtcg ccctcgaagg gagcgtcaag ggcgatgtcg cgatggagga
    11521 cctcgacgct cacgtgctca ccggcgagct ccgtgattgt ggcgagcagc tgctcctcgt
    11581 gaccgggcag gaaccggcag tcgacgttgg cgctggcgtg ctgcgggatg acgttgtgct
    11641 tgtagcccgc gtcgagcatg gtgacgttgc tcgtgtcctg aagggtgccg cgcacgaagc
    11701 cctgtgcccc gcgaagatgg tcgagcaggt cctcgaggtc ctcgtcggac cagctcgtgc
    11761 ccgtcagctc acccaggccc tcgagcaagg tgcgcaccga cgggatgaac tcgcggggcc
    11821 aggtgtgggc ggcgatgcgg gagatggcct cggccagccg cacgatggcg ttctcgtcgt
    11881 tggggaccga accgtggccc gcgcggccgt gagcggtcag gcgaagccag cagatgccct
    11941 tctcggccgt ctggagcagg taggtgcgac gctcgtcgcc atggcgatcg gtcacggtga
    12001 cgctgtagcc gccgacctcg ctgatcgcct cggtgacccc gtcgaagacc tcggggtgct
    12061 gctcgacgag gaagtgcgaa cccttgcgcc cgccggcctc ctcgtccgcg aagaacacga
    12121 tgacgaggtc gcgcggcgga acggtgccgg tgcgggcgag gtcgcggaca caggcgagga
    12181 tcatcgcgtc catgtccttc atgtcgacgg cgccgcgccc ccagacgcag ccgtcacgca
    12241 gctcacccga gaacgggtcg accgaccagt cggcggcgtt ggccggaacg acgtcgaggt
    12301 ggccgtggat gcacagggcg ccgcgctcac ggtcggcgcc ggggatgcgg accaccacgc
    12361 tgacccgccc cgggtcgctc tcgaacgtct gcggttccag ccccacctct cgcaggctct
    12421 gcacgacgta ggcagcggcc tcggcctcgc cgggtcccga accgtcgccg aagttgctcg
    12481 tgtcgatccg gatcaggtcc tggcagatct ggacgacctc gtcctgggct gctgtcgtgg
    12541 tctcacgcat gaggacacac taggccgggg ccgcgatcgg gcggcggcag aggacgtccg
    12601 gggatcggat ttcggtccga cgcacgatgc gtgtaatgtt ccgggtcgca ggacggcctc
    12661 cccgggccgg gcccgcacca ctcgtccggg tggcggaatt ggcagacgcg ctagcttgag
    12721 gtgctagtgc cc
//
LOCUS       DAOSOC010000058        12728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_4928_length_12728_cov_9.502486, whole genome shotgun sequence.
ACCESSION   DAOSOC010000058 DAOSOC010000000
VERSION     DAOSOC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12728
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4928_length_12728_cov_9.502486"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..686
                     /locus_tag="PL014_06170"
     CDS             72..686
                     /locus_tag="PL014_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56712.1"
                     /translation="MSATRETVTLPCTKCGGSGVLPEFRGVDGGRCWACGGTRQRTIL
                     AASARARRRRQAARAVERAAHAAANATDREQSWARAREALRPLVGDRVDLADNSGITV
                     AGYPDGEARLRGHLFDAILRVRDGADPAAEAAWIRDQVEGAVLWINRVRRQCIACEVA
                     VGRGQGIVLEVGNDQWVLCSHHRPTGLAEASEVDSSRPSSSAWR"
     gene            772..999
                     /locus_tag="PL014_06175"
     CDS             772..999
                     /locus_tag="PL014_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOT56713.1"
                     /translation="MTTSIRIPEETQRRLDDLAARTHRSRSFYLREAIERGLPQVEWE
                     YDIAQRAGEARSGAVATETLAEVVAELGLDR"
     gene            983..1255
                     /locus_tag="PL014_06180"
     CDS             983..1255
                     /locus_tag="PL014_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT56714.1"
                     /translation="MAWTVELHPDASKSLRTLARKDRQMAKRVTHFLAEIERSEDPRA
                     RGKGLSGPLTGLWRYRVGDWRVVVDIYDDRLVILALDIEHRSRAYR"
     gene            1404..1829
                     /locus_tag="PL014_06185"
     CDS             1404..1829
                     /locus_tag="PL014_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56715.1"
                     /translation="MLSRPDISLADRALGQGQPAAVTNIDQRVGVLAHTVTNHGALQV
                     GDNNTQTVTLTREQSSQLLSQLPELRELISAVNLPREISDRLESDIHELEQVAEHGGG
                     VGRARALLGDMVEASAVAASTDAGQRLGALLFSLMGGLA"
     gene            complement(2033..2863)
                     /gene="fdhD"
                     /locus_tag="PL014_06190"
     CDS             complement(2033..2863)
                     /gene="fdhD"
                     /locus_tag="PL014_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOT56716.1"
                     /translation="MGRVTTRVRRTRLDVVSGARVERTDTVAVEEPLEIRVDNTSLTT
                     TMRTPGDDFDLAIGHLLTEGLLHGADEVATLMHCTDLDESGSPTFNVVDVTLAPGAVL
                     HRAPRERTQGMTSACGVCGSASVDAVRTSSRYAVAGDPLSLSAEVVAGLPDVLRQQQP
                     VFERTGGVHAAGLATASGDLLHVREDVGRHNAVDKVVGAAARVGSLPLGGHVLVVSAR
                     ASFEIIQKASVVGIPAVVAVGAPTSLAVDLAVDRGITLVAFTRHPSMSIYSRPDRLAT
                     "
     gene            2954..3772
                     /locus_tag="PL014_06195"
     CDS             2954..3772
                     /locus_tag="PL014_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT56717.1"
                     /translation="MDTNPEIGLSIDVDGITTNYHRAGSGPVVLLVHGSGPGVSAWAN
                     WRLTIPALAEHFTVIAPDIVGFGFTDRPEGFDYSLDTWRAHLLGFADALGLDRFSIVG
                     NSFGGALALSMAAHQPERVDRLVLMGAAGVPFPITDGLDRVWGYEPSLETMRGLLDVF
                     AHDPAFATDELAALRYEASVRPGFQESFAAMFPAPRQRWVDALATPDDVIAALPHRAL
                     VVHGRDDQVIPLANAHHLLELIDDSQLHVFGRCGHWTQIEHAAEFNALVARFLR"
     gene            3787..4305
                     /locus_tag="PL014_06200"
     CDS             3787..4305
                     /locus_tag="PL014_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT56718.1"
                     /translation="MDDPDPATSVSPVQVTLHGGDLPWQPVSPNLTRVRLITLALFAV
                     PAIIATVVVGVLVTRWALLGTAAVVLVALWVAWLISRQVSAISWVELDEEIVIRKGRL
                     FRSLVSVPYGRLQYVDIQSGPLMRAHGIASCEIHTASPESGGSLPGLPVAEAEALRER
                     LSARGEAQRAGL"
     gene            4302..5894
                     /locus_tag="PL014_06205"
     CDS             4302..5894
                     /locus_tag="PL014_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT56719.1"
                     /translation="MTEAVIRDEEWQRLHPLSPLLRGGVVLIAVIGYVASQFFDRFLS
                     SINLDGILPGPDIGIGPAGEDGALEQVVAHPFLALLALVLLVAGVAGVNWVAWRFSRF
                     RVAGGQVELRTGVLFRQHRQVRLERVQAVELSRPILARLFGLASVVVQSAGGSDSQLA
                     LSFIDLRRAEALREHLLTLAGQSEVVLAPTDDSMATPPTMGDAVPSPRAPVVAVPVVE
                     VPNGRLFVATILHGSTIFLGAVLFGGLITAGVLSVGPLALAGVPAMLPAAFFIVLGRG
                     KEMLTHGNFRLVDVGTGVRVTEGLTDLRTMTIPLNRVQALEVIQPIWWRPLGWWRVRV
                     NVAGVGSSGSGDGEGERETTLLPVGTLDDALRVLTLLAPRVPASRWNDAVLGEGPQRG
                     WQTSSARAKLFDPLSWRRNGWSDTDGAVLLRSGRLTRRAIAVPHARIQSLTLQQGWLQ
                     ASRRVATLHVIPAPGPISPVIDHLDTEAAHDAFDRLNERARDARRGAGPIDPPLAPDP
                     AGLVDWTQHPSGVPEPGRPDPL"
     gene            5891..6334
                     /locus_tag="PL014_06210"
     CDS             5891..6334
                     /locus_tag="PL014_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HOT56720.1"
                     /translation="MNTPAPPPVDPGLNGSTAVARLGFTRDQVVQEFGYDSDVDDEFR
                     FAVEDLCGAEMEDEDYTGGADAVLLWFRDGDGDLGDALVDMVGVLDDGGFVVLLTPKA
                     SAEEVDASDIDEAAVTSGLHPAGTFTASEHWRAHKLVAPKAPAKR"
     gene            6410..6889
                     /locus_tag="PL014_06215"
     CDS             6410..6889
                     /locus_tag="PL014_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT56721.1"
                     /translation="MPLSTLNPLAVGLRAPDFALRDQNGVEVTLSGLTTEKNVVIVFY
                     PFAFSGICTGELDEIRDHLERFVSDDLQVVCISSDPVFSQRAWADQQGYFFPLLSDFW
                     PHGEVAQAYGVLNERTGAPVRGTFLVGRDGFVKWSLINGPGDRRDFSDLPAEVAALP"
     gene            6972..7044
                     /locus_tag="PL014_06220"
     tRNA            6972..7044
                     /locus_tag="PL014_06220"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7005..7007,aa:Val,seq:tac)
     gene            7046..7897
                     /locus_tag="PL014_06225"
     CDS             7046..7897
                     /locus_tag="PL014_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT56722.1"
                     /translation="MSDDADLTLREVLDVIEGLYPPHTAQSWDRVGLVTGDPHQLVRR
                     IHLAVDPTLDVIAEARELGADLLLTHHPLLMRGVHSLATTSAKGASVTDLIVGDVALY
                     VAHTNADVAADGVGFALARACGLTDTEPLTLAEGQPLGRVGDLPDAVSLRALVVALAA
                     ALPAAAGGIRVAGPADAVVRRVALLGGSGDDLFDAVRASGADVYVTADLRHHPALEAR
                     EEARGGTPYLVDAGHWASEFVWLAAAERALLAALGERATRVETHISTVRTDPWTFVVG
                     ADPGGTP"
     gene            7894..8634
                     /locus_tag="PL014_06230"
     CDS             7894..8634
                     /locus_tag="PL014_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOT56723.1"
                     /translation="MNAETARQLRLLDLQAIDTRLDQIEHARTHLPQLAELADLSGKA
                     QLIDDQLVRSRTELDDISREVAKADADVQQVRDRVARDQARLDAGTGSAKDLTAIQHE
                     LVSLARRQAELEDIELEVMERAENAQSDVSELERGRGELTVRIQALEAARDDALRVLD
                     AEAAEVAGPRGTVAAEAGDDLVALYEKIRSANGGIGAAALRQRRCGGCQLELNPVEIQ
                     RIRVAPEDEVLRCEECRRILVRTPESGL"
     gene            8631..9578
                     /locus_tag="PL014_06235"
     CDS             8631..9578
                     /locus_tag="PL014_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HOT56724.1"
                     /translation="MRRRLTVEADGGSRGNPGVAGYGALVRDTETGALLAERAEPLGK
                     ASNNVAEYRGLIAGLEAAHAIDPGAEVLARMDSKLVVEQMSGRWKIKHEDMRRLALQA
                     RDIAARITSAGGTVRYEWIPREINKDADALSNVAMDGRTVNRTFAESESAGQEPAGEL
                     DAAGDAAAAAGGEADAGEAVRVVLVAAAASRDAAGATASAVLSVVGTGGIEVVGGVAD
                     AVDVTARAIGDALGTEVEVDPAWDSAGPGARAAWARAVARGGTVVAVCPVDVVRGVLA
                     DLLGVPGERQDRLAVAPGSLAGVEVWGERRVSVAFTNRT"
     gene            9623..10036
                     /locus_tag="PL014_06240"
     CDS             9623..10036
                     /locus_tag="PL014_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT56725.1"
                     /translation="MSYAGDVTPEQAHAAVTGPEGAMLVDVRTRAEWSFVGVPVIDGA
                     ADAVAFVEWSTFPAGTVNERFVDEVVAAGLAPGRPVYLLCRSGVRSKAAADALTAAGL
                     GPAYNILDGFEGGHDADGHRTVSGWKNAGLPWRQG"
     gene            10033..11250
                     /locus_tag="PL014_06245"
     CDS             10033..11250
                     /locus_tag="PL014_06245"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HOT56726.1"
                     /translation="MNDPVAHWHPQTRAVRGGLSRSGFDETAEALYLTSGFVYESAEQ
                     AEAAFKDEVEHYIYSRYGNPTVSTFEERMRQLEGAEACFATASGMAAVFTAMAALCGA
                     GDRVVASRALFGSCFVILDEILPRWGVETVFVDGADLDQWREALSVPTTAVFFETPSN
                     PMMELVDIAAVSELAHAVGATVVVDNVFGTPVFSRPLEHGADVVIYSTTKHIDGQGRA
                     LGGAILGTAEFVNGPVKNLMRHTGPAMSPFNAWVMTKGLETLGLRVRAMADSALTVAQ
                     TLEEWQHSGRGITRVVYPHLASHPQHELAVRQLEGGGGTVVTFDVAGGKAAAFAFMNA
                     LTLVDISNNLGDSKSLTTHPATTTHRRLAPEARAAVGITDGTIRVSIGLEDERDLIAD
                     IDTALAAADAVAR"
     gene            11273..11737
                     /locus_tag="PL014_06250"
     CDS             11273..11737
                     /locus_tag="PL014_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT56727.1"
                     /translation="MSDVLSVQRLIPSPPEPIFDLLVDPAGHSEIDGGGTVKGARSGG
                     RRLVLGDRFGMDMKLGVAYSTRNTVIELEENRRIAWQTTASGPAGSLLGGRIWRYVLE
                     PVEGGTLVTESWDLTQERVTSKFVVKATMTDATKRNMERTLARIEELVTSVG"
     gene            complement(11744..12634)
                     /locus_tag="PL014_06255"
     CDS             complement(11744..12634)
                     /locus_tag="PL014_06255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HOT56728.1"
                     /translation="MLPVTDLSRRAQQRLGAALRARVAGDDAAQRAAVIWGKPGQRWF
                     TPQDPVWRVHADASMFPGGVAALLLQSLHPLAMAGVAGHSGYRGDPWGRLQRTSHFLA
                     TTTFGTIADAEQTIARVRSIHERVRGRDERGRPYRASDPDLLSWVHVAEAYSFLTAYQ
                     RFAVEPLTATEADTYVEQAAVVARLLGATEVPTTVAGLERALTAYRPDLEATDAARDA
                     ARFLLLQPPLPLLARPGYSLITTGGVAVLPGWARSSLGLPMARPLAPVATGIGRFGTR
                     AVRWAMAGVAQERQLAADLT"
BASE COUNT         1769 a         4521 c         4542 g         1896 t
ORIGIN      
        1 ctcgtcgacg acgggcggca cgagtgctgg ctcgatacgg ccccgcggca tctagctttg
       61 gacccagcct catgagcgcc actcgtgaaa ccgtgaccct gccgtgcacg aagtgcggcg
      121 ggtcgggtgt cctgcccgag ttccggggcg tcgacggcgg gcgctgctgg gcgtgcggcg
      181 gcaccaggca acgcaccatc cttgccgcct ccgcacgggc ccgtaggcgc cgccaggctg
      241 cacgcgctgt cgagcgcgca gctcacgccg cggccaacgc aacggaccgt gagcagtcct
      301 gggcccgggc tcgagaggcg ttgcgccccc tggttggcga ccgggtcgac ctcgcggaca
      361 actccggcat cacggtcgcg gggtacccgg acggagaggc gcgcctcagg ggccatctgt
      421 tcgacgcgat cctccgggtt cgagatggag cagaccctgc tgccgaggcc gcgtggatcc
      481 gcgaccaggt cgaaggagcc gtgctgtgga tcaaccgggt ccgccgccag tgcatcgcct
      541 gtgaggtggc cgtgggccgc ggccagggca tcgtcctcga ggtgggtaac gaccagtggg
      601 tgctgtgctc ccaccaccgc cctacgggac tggccgaggc gtcagaagtt gattcatcga
      661 ggccctcgtc gagcgcgtgg cgctgatgtg gcaccccggg tgagcgaggc cctggccttg
      721 ccagtgtgac tacactgtac tcatccgaat acatggcttt caggagtggt gatgaccacg
      781 agcattcgca tccccgagga gacgcagcgc cggctcgacg accttgccgc caggacgcac
      841 cggtcccggt cgttctacct gcgcgaggcg atcgagcgcg gactgccgca ggtcgagtgg
      901 gagtacgaca tcgcgcaacg agccggcgag gctcgatccg gtgcggtggc caccgagacc
      961 ctggccgagg tggtggcaga acttggcttg gaccgttgag ctgcacccag acgcctccaa
     1021 gtccttgcgc accctggccc gcaaggaccg gcagatggcc aagagggtca cccacttcct
     1081 ggccgagatc gaacgctccg aggacccccg ggcacgcggg aagggactct ccggcccgct
     1141 gacaggcttg tggcggtacc gagtcggaga ctggcgcgtt gtggtcgaca tctacgacga
     1201 ccgcctggtc atcctggccc tcgacatcga acaccgatca cgggcgtacc gttgaccact
     1261 gctcaagggt ggcgcaccgg tcgttgctga ggacgagagg atccgagcgc tcgccgcgct
     1321 cgagcgcttg ggttgcctca cagcgagaca ttcatttcag gatgagtact tccaatgtga
     1381 ggtgaccccg ctgggaagtg gacgtgctct cgcgtccgga catctccctg gcggatcggg
     1441 ctctgggcca gggccagccg gcagccgtca cgaacatcga ccagcgggtc ggtgtcctcg
     1501 cgcacaccgt cacgaaccat ggcgcacttc aggtgggaga caacaacact cagaccgtga
     1561 cgctcacgcg agagcagtcc tcccagctcc tctcgcagct gccggagttg cgggagttga
     1621 tctccgccgt gaacctgccg cgcgagatct ccgacaggct tgagtccgat atccatgagc
     1681 ttgagcaggt cgcggagcac gggggtgggg tcgggagggc gagggctctt ctgggggaca
     1741 tggtggaggc gtctgcggtc gcagccagca ccgacgcggg tcagcgactg ggggcgctcc
     1801 ttttcagcct catgggtggc ctcgcttgat ccaccgccgt gggggcgggc gggtcatcgg
     1861 cggcgttttg agcgcaggcg gatgcggatg cggatggtga ggtttgcggt gattccgcag
     1921 gggcactcga cgagtgcaag catcgcacaa ggtctccact gttttcgggc tcatcccaat
     1981 cgagggtgta gcggtcgtgg tatagatcct tacgcccccg ctgcgtgacg cgtcacgtag
     2041 ccaggcgatc ggggcggctg tagatcgaca tgctcggatg ccgggtgaac gccacgagcg
     2101 tgatgccgcg gtccacggcc aggtcgaccg ccaacgacgt cggggccccc accgcgacga
     2161 cggccgggat gccgacgacg ctcgccttct ggatgatctc gaagctcgcc cgggcgctga
     2221 cgacgagcac gtgcccgccc agcggcagtg accccacgcg ggccgcggcc ccgacgacct
     2281 tgtcgacggc gttgtgccgc ccgacgtcct cgcgcacgtg cagcaggtca ccggatgccg
     2341 tcgcgagacc ggccgcgtgg accccgccgg tgcgctcgaa gacgggttgc tgctggcgca
     2401 ggacatccgg gagcccggcc accacctccg ccgagaggga gagcgggtcg cccgccacgg
     2461 cataccgact gctcgtgcgc acggcatcca cggacgccga cccgcagacc ccgcacgcgc
     2521 tggtcatgcc ctgcgtgcgc tcacgcgggg cgcggtgcag cacggcgccc ggtgccaggg
     2581 tgacgtcgac gacgttgaag gtgggggatc cgctctcgtc gaggtccgtg cagtgcatga
     2641 gcgtcgcgac ctcgtcggcg ccgtggagca ggccctccgt gagcaggtgc ccgatcgcga
     2701 ggtcgaagtc gtcgccgggt gttcgcatcg tcgtcgtgag tgaggtgttg tcgacgcgga
     2761 tctccagcgg ctcctcgacg gcgacggtgt ccgtgcgctc gacccgggca ccgctgacga
     2821 cgtcgagtcg tgtccgtcga acgcgcgtcg tcacccggcc catgggcgtc atccaaacac
     2881 acggacaccg cggtgttacg gggtgcggaa cggtggcagc gctccacacc tgaccgggcc
     2941 taccgtcagc cgcatggaca ccaaccccga gatcggcctg tcgatcgacg tcgacggcat
     3001 cacgacgaac taccaccggg cgggcagcgg ccccgtcgtg ctgctcgtgc acggttcggg
     3061 cccgggggtc tccgcgtggg cgaactggcg gctgacgatc ccggccctgg ccgagcactt
     3121 cacggtcatc gcccccgaca tcgtcggttt cggcttcacc gaccggcctg aggggttcga
     3181 ctactccctg gacacgtggc gggcccacct gctcggcttc gccgacgccc tcgggctcga
     3241 ccgcttctcg atcgtcggca acagcttcgg cggcgcgctg gccctgtcga tggccgccca
     3301 ccagcccgag cgggtcgacc gactcgtgct catgggtgcc gccggggtgc ccttccccat
     3361 caccgacggt ctcgaccggg tctggggcta cgagccgtcg ctggagacga tgcgtgggct
     3421 gctcgacgtg ttcgcgcacg acccggcctt cgccaccgac gagctcgcgg cgctgcggta
     3481 cgaggcctcg gtgcggccgg gcttccagga gtccttcgcc gcgatgttcc ccgcgccgcg
     3541 gcaacgctgg gtcgacgccc tcgccacccc ggatgacgtc atcgcggccc tgccccaccg
     3601 cgccctggtc gtccatggcc gcgacgacca ggtcatcccg ctcgccaacg cccaccacct
     3661 gctcgagctc atcgacgaca gccagctgca cgtcttcggc cgctgcgggc actggaccca
     3721 gatcgaacac gccgccgagt tcaacgctct ggtggcgcgc ttcctgcgct gaggcagact
     3781 ggggccatgg atgaccccga tcccgcgacg tccgtgagcc cggtgcaggt cacccttcac
     3841 gggggtgacc tgccgtggca gccggtgtcc ccgaacctga cccgggtgcg tctcatcacc
     3901 ctcgccctgt tcgcggtgcc ggcgatcatc gccacggtcg tcgtcggggt gctggtgact
     3961 cgctgggcgc tgctcggcac ggcagccgtc gtgctcgtcg cgctctgggt ggcgtggctc
     4021 atcagccgac aggtgagtgc gatcagctgg gtcgagctcg acgaggagat cgtcatccgc
     4081 aagggccggc tcttccgctc gctcgtcagc gtgccctacg gccgcctcca gtacgtcgac
     4141 atccagtcgg ggccgttgat gcgcgcccac ggcatcgcct cgtgcgagat ccacaccgcc
     4201 tcgccggaga gcggtgggtc gctgcccggc ctgccggtcg ccgaggccga ggccctgcgc
     4261 gagcgcctgt ccgcgcgcgg cgaggcgcaa cgggccggcc tgtgaccgag gccgtcattc
     4321 gggacgagga gtggcagcgg ctgcacccgc tgtcgcccct gttgcgcggc ggcgtcgtgc
     4381 tcatcgcggt catcgggtac gtcgccagcc agttcttcga ccgcttcctg agctccatca
     4441 atctggacgg catcctgccg gggccggaca tcgggatcgg gccggctggg gaggacggcg
     4501 ccctcgagca ggtcgtcgcc cacccgttcc tcgccctgct cgccctcgtg ctgctcgtcg
     4561 ccggggtcgc cggcgtcaac tgggtcgcct ggcgcttctc ccgcttccgg gtggcagggg
     4621 gccaggtcga gctgcgcacc ggcgtgctct tccgccagca ccgacaggtg cggctcgagc
     4681 gggtgcaggc cgtcgagctg tcccgcccga tcctggcccg gctgttcggg ctggccagcg
     4741 tcgtcgtgca gtcggccggt ggttcggact cccagctcgc cctgtcgttc atcgacctca
     4801 gacgtgccga ggcgctgcgt gagcacctcc tgaccctcgc gggccagtcc gaggtggtgc
     4861 tggctccgac tgatgactcg atggcaaccc ccccgaccat gggggatgcc gtgccgtcgc
     4921 ctcgagcgcc cgtcgtcgcc gtcccggtcg tcgaggtgcc gaacgggcga ctgttcgtcg
     4981 cgacgatcct gcacggctcg acgatcttcc tcggggccgt gctgttcggt gggctgatca
     5041 ccgcgggggt gctcagcgtc ggcccgctgg ccctcgccgg ggtgccggcc atgctgccag
     5101 cggcgttctt catcgtcctc ggccgaggca aggagatgct gacccacggc aacttccgcc
     5161 tcgtcgacgt cggcaccggg gtccgggtga ccgagggact gacggacctg cggacgatga
     5221 ccatcccgtt gaaccgggtc caggcgctcg aggtcatcca acccatctgg tggcgcccgc
     5281 tcggctggtg gcgggtgcgc gtcaacgtcg ccggggtggg ctcctcgggc agcggcgacg
     5341 gcgaagggga gcgcgagacg acgttgctgc ccgtcggcac gctcgacgac gccctgcggg
     5401 tgctcaccct gctcgctccg cgggtgcccg cctcgcggtg gaacgacgcg gtcctcggcg
     5461 aggggccgca gcggggctgg cagaccagct ccgcacgggc caagctgttc gatccgctct
     5521 cgtggcggcg caacggctgg tcggacaccg acggggccgt cctgctgcgc tccgggcggc
     5581 tcacgcggcg cgccatcgcc gtgccgcacg ctcgcatcca gtcgctgacg ctgcagcagg
     5641 ggtggctgca ggcgagtcgc cgcgtggcga ccctgcacgt catccctgct cccggcccca
     5701 tcagccccgt catcgaccac ctcgacaccg aggccgccca cgacgccttc gatcgcctca
     5761 acgagcgggc ccgtgacgcc cgccgagggg cgggaccaat tgaccccccg cttgcacccg
     5821 acccggccgg gttggtggac tggacccagc acccgagcgg tgtccccgaa ccaggaaggc
     5881 ccgacccact gtgaacactc ccgccccgcc gccggtcgac ccggggctga acggctccac
     5941 cgccgttgcg cgcctcggct tcacccgcga ccaggtggtg caggaattcg gttacgacag
     6001 tgatgtcgac gacgagttcc gcttcgccgt cgaggacctc tgtggcgccg agatggagga
     6061 cgaggactac accggtggtg cggatgccgt gctgctgtgg ttccgcgacg gcgacggcga
     6121 cctgggcgac gccctcgtcg acatggtcgg cgtgctcgac gacggcgggt tcgtcgtcct
     6181 gctcaccccc aaggcgtcgg ccgaggaggt cgacgcgagc gacatcgacg aggccgcggt
     6241 cacctcggga ttgcaccccg cagggacctt caccgcctcc gagcactggc gtgcgcacaa
     6301 gctcgtggca cccaaggcgc cggccaagcg gtagcccgcg cacggcatcc gtgcgtctgt
     6361 cccgtctcag tgggtctccg gtggggcagg tcgctggcag gatggaccca tgccgttgtc
     6421 gaccctgaac cccctagccg tcggcctgcg cgccccggac ttcgccctgc gtgaccagaa
     6481 cggtgtcgag gtgaccctct cggggctgac gacggagaag aacgtcgtca tcgtgttcta
     6541 ccccttcgcg ttctcgggca tctgcaccgg cgagctcgac gagatccgcg accacctcga
     6601 gcggttcgtc tccgacgacc tccaggtggt ctgcatctcc agcgaccccg tgttctccca
     6661 gcgtgcctgg gccgaccagc agggctactt cttcccgctg ctctccgact tctggccaca
     6721 cggcgaggtc gcccaggcct acggcgtgct caacgagcgc accggcgccc ccgtgcgggg
     6781 gaccttcctc gtcggccgcg acgggttcgt caagtggagc ctgatcaacg ggcccggcga
     6841 ccgccgcgac ttcagcgacc tccccgccga ggtcgccgcc ctgccctgac ggcacgcctg
     6901 acacgggtgc gcgcccacct gccacaatgt gcgcggacat cagtgctcct tcgggggtcg
     6961 agggtgtcca ggggcctgta gctcagctgg tagagcgccg cgtttacacc gcgaaggtcg
     7021 tcggttcgat cccggccggg cccacatgag tgacgacgcc gacctgaccc tgcgggaggt
     7081 gctcgacgtc atcgagggcc tctacccgcc gcacaccgcg cagtcgtggg accgggtcgg
     7141 gctggtcact ggggacccgc accagctcgt gcggcgcatc cacctggcgg tcgacccgac
     7201 cctggacgtc atcgccgagg cccgcgagct gggcgccgac ctgctgctca cccaccaccc
     7261 cctcctgatg cggggggtgc acagcctcgc cacgaccagt gccaaggggg cgagcgtcac
     7321 cgacctcatc gtcggcgacg tcgcgctgta cgtcgcccac accaatgccg acgtcgcggc
     7381 agacggcgtg ggcttcgccc tcgcgcgggc ctgcggcctg accgacaccg agccgctcac
     7441 gctggccgag gggcagccgc tcggacgggt gggggatctc cctgatgccg tgtcgcttcg
     7501 cgctctcgtc gtggcgctcg ccgccgccct tccggcggcc gcgggcggca tccgggtggc
     7561 cggaccggcg gatgccgtgg tgcgccgggt cgcgctgctc ggcgggtcgg gtgacgacct
     7621 gttcgacgcg gtgcgcgcca gtggtgccga cgtgtacgtg acggcagacc tgcgccacca
     7681 ccccgccctc gaggcccggg aggaagcccg cggaggaacc ccgtacctcg tggatgccgg
     7741 gcactgggcc agtgagttcg tctggttggc agctgcggag cgggcgctgc tcgcagccct
     7801 gggggagcgc gccactaggg tggagaccca catcagcaca gtgcgcaccg acccctggac
     7861 cttcgtcgtc ggtgccgacc ccggaggtac cccgtgaacg ccgagaccgc ccggcagctg
     7921 cggctgctcg acctccaggc catcgacacc cggctggacc agatcgagca cgcccgtacc
     7981 cacctaccgc agctggccga gctcgccgac ctgtcgggca aggcccagct catcgacgac
     8041 cagctggtgc gctcgcgcac cgagctcgac gacatcagcc gcgaggtggc caaggcagac
     8101 gccgacgtcc agcaggtgcg cgaccgcgtc gcccgtgacc aggcccgcct cgacgccggc
     8161 accgggtcgg ccaaggacct caccgccatt cagcacgagc tcgtctcgtt ggcccgccga
     8221 caggctgagc tggaggacat cgagctcgag gtcatggagc gtgccgagaa cgcgcagtcc
     8281 gacgtctccg agctcgagcg cggccgcggc gagctgacgg tgcgcattca ggctctcgaa
     8341 gctgcccgcg acgacgccct tcgcgtgctc gacgcggagg ccgccgaggt cgccgggccg
     8401 cgcggcactg tcgcagcgga ggcgggtgac gacctggtcg ccctctacga gaagatccgg
     8461 tccgcgaacg ggggcatcgg cgccgctgcg ctgcgccagc gcaggtgcgg aggctgccag
     8521 ctcgagctca accccgtcga gatccagcgc atccgggtcg cgcccgagga cgaggtgctg
     8581 cgctgtgagg agtgccggcg catcctcgtc cgcacccctg agtccggctt gtgaggcgac
     8641 ggctcaccgt cgaggccgac ggcgggtcgc gcgggaaccc gggcgtcgcc ggatacggcg
     8701 ctctcgtgcg cgacaccgag accggtgcgc tgctcgccga gcgcgccgaa ccgctcggca
     8761 aggcctcgaa caacgtcgcc gagtaccgcg ggctcatcgc cggcctggag gccgcccacg
     8821 cgatcgaccc cggggccgag gtgctcgccc gcatggactc caagctcgtc gtcgagcaga
     8881 tgtcgggccg gtggaagatc aagcacgagg acatgcgtcg cctcgcgctc caggcccgtg
     8941 acatcgccgc gcggatcacc tcggccggcg gcaccgtgcg ctacgagtgg atcccgcggg
     9001 agatcaacaa ggacgccgac gccctgtcca acgtcgccat ggacggccgc accgtcaacc
     9061 ggacgttcgc cgagtcggaa tcggctggcc aggaaccggc cggggagctg gatgcggctg
     9121 gtgacgccgc cgccgccgcc ggtggcgagg ccgacgccgg cgaggcggtt cgcgtggtgc
     9181 tcgtcgccgc ggcggcgagc cgggacgcgg cgggggccac ggcatccgcg gtgctgtcgg
     9241 tcgtcggcac cggcggcatc gaggtcgtcg gtggggtcgc ggatgccgtc gacgtcaccg
     9301 cccgcgcgat cggtgacgca ctcggcaccg aggtcgaggt cgatcccgcg tgggactccg
     9361 ccgggcccgg tgccagggcc gcctgggctc gagcggtcgc ccgtggcggg acggtcgtgg
     9421 ccgtctgccc cgtcgacgtc gtgcgtgggg tgctggccga ccttctcggc gtgcctggcg
     9481 agcggcagga ccgccttgcc gtcgcgccgg ggtcgctggc cggggtcgag gtctggggtg
     9541 agcggcgggt ctccgtggcg ttcaccaacc gcacctgaga cacggtgtcg aggggggtgg
     9601 caccccgcct acgctgaagg gcatgagcta cgcaggtgat gtcacacccg agcaggccca
     9661 cgcagcggtg accggtcccg agggggccat gctcgtcgat gtccggaccc gggccgagtg
     9721 gtcgttcgtc ggcgtgccgg tgatcgacgg cgctgcggat gccgtggcgt tcgtcgagtg
     9781 gtcgacgttt cccgccggca cggtgaacga gcgcttcgtc gacgaggtcg tcgcggccgg
     9841 gctcgccccg ggacgcccgg tgtacctgct ctgccgctcg ggcgtgcgat ccaaggcggc
     9901 ggcggacgcc ctgaccgcgg ccggcctggg ccctgcgtac aacatcctcg acgggttcga
     9961 gggcggccac gacgccgacg ggcaccgcac ggtgtcgggg tggaagaacg ccggcctgcc
    10021 gtggcgccag ggatgaacga ccccgtggcg cactggcacc cacagacgcg cgctgtgcgc
    10081 ggtgggttga gtcggagcgg gttcgacgag acggccgagg cgctctacct gacctcgggc
    10141 ttcgtctacg agtcggccga gcaggccgaa gccgcgttca aggacgaggt cgagcactac
    10201 atctacagcc gttacggcaa ccccaccgtc tcgaccttcg aggagcggat gcgccagctc
    10261 gagggcgcgg aggcgtgttt cgcgacggcc tcggggatgg ctgccgtgtt cacggcgatg
    10321 gcggccctgt gcggggcggg ggaccgggtg gtggcgtccc gagcgctgtt cgggtcgtgc
    10381 ttcgtcatcc tcgacgagat tttgccgcgg tggggtgtcg agacggtctt cgtcgacggc
    10441 gctgacctcg accagtggcg cgaggcgctg tcggtgccga cgacggcggt cttcttcgag
    10501 acgccgagca acccgatgat ggagctcgtc gacatcgcgg ccgtctccga gctggcgcac
    10561 gcagtcgggg ccaccgtcgt cgtcgacaac gtcttcggca ccccggtgtt ctcgcgccct
    10621 ttggagcacg gcgccgacgt cgtcatctac tcgacgacca agcacatcga cggccagggg
    10681 cgcgccctcg gcggtgcgat cctcgggacg gccgagttcg tcaacggacc ggtgaagaac
    10741 ctcatgcggc acaccgggcc ggccatgtcg ccgttcaacg catgggtcat gaccaagggg
    10801 ctcgagaccc tcggcctgcg ggtgcgagcc atggcggact cggcgctgac ggtggcgcag
    10861 acgctcgagg agtggcagca ctccggtcga ggcatcaccc gggtggtcta cccgcacctg
    10921 gcgagccacc cccagcacga gctggcagtg cgtcagctcg agggcggggg cggcaccgtc
    10981 gtgacgttcg acgtcgcagg cggcaaggcg gcggcgttcg ccttcatgaa cgccctcacc
    11041 ctggtcgaca tctcgaacaa cctcggggac tcgaagtcgc tgaccaccca cccggccacg
    11101 accacccacc gtcgcctcgc ccccgaggcc cgggcggcgg tcggcatcac cgacggcacc
    11161 atccgggtgt ccatcggtct cgaggacgag cgcgacctca tcgccgacat cgacacggca
    11221 ctggcggcag ccgatgccgt ggcccgctga cgcgcctgtt agcgtcgccg gcatgagtga
    11281 tgtcctgtcc gtccagcgcc tgatcccctc acctcccgag ccgatcttcg accttctcgt
    11341 cgacccggcc ggtcactcgg agatcgacgg tggcggcacc gtcaaggggg cccgcagcgg
    11401 cgggcgtcgg ctcgtgctcg gcgaccgctt cggcatggac atgaagctcg gtgtggccta
    11461 ctcgacgcgc aacaccgtca tcgagctcga ggagaaccgg cggatcgcct ggcagacgac
    11521 ggcatcgggc ccggccgggt cgttgctggg cgggcgcatc tggcgctacg ttctcgagcc
    11581 ggtcgagggc ggcacgctcg tcacggagtc ctgggacctg acccaggagc gggtcacgag
    11641 caagttcgtc gtcaaggcga cgatgaccga cgccaccaag cgcaacatgg agcgcacgct
    11701 ggctcgcatc gaggagctcg tgacctcggt cgggtgaccg tcctcaggtg aggtcggcgg
    11761 cgagctggcg ttcctgcgcc acccctgcca tggcccagcg caccgctcgc gtcccgaagc
    11821 ggccgatgcc cgtcgcgacc ggtgcgagcg ggcgcgccat cggcaggccg agcgagctcc
    11881 gggcccagcc gggcagcacg gcgactcctc ccgtggtgat caggctgtac cccggtcgcg
    11941 ccagcaacgg cagcggaggt tgcagcagca ggaagcgggc ggcatcacgt gctgcatcgg
    12001 tggcctcgag gtcggggcgg tatgccgtga gcgcacgttc cagcccggcc accgtcgtcg
    12061 gcacctcggt ggccccgagc aggcgtgcga ccacggcggc ctgctcgacg taggtgtcgg
    12121 cctcggtggc cgtgagcggc tccacggcga agcgctggta ggcggtgagg aacgagtaag
    12181 cctcggcgac gtgcacccac gacaggaggt cggggtcgct cgcgcggtag ggccgacccc
    12241 gctcgtcacg accgcgcacc cgctcgtgga tggagcggac tcgcgcgatg gtctgctcgg
    12301 cgtccgcgat ggtgccgaac gtcgtcgtgg ccaggaagtg ggaggtgcgc tggagccgac
    12361 cccacgggtc tccgcggtag ccgctgtgac cggcgacccc ggccatggcc aggggatgca
    12421 gcgactggag gagcagcgcg gcgaccccac cggggaacat cgacgcgtcg gcgtgcaccc
    12481 gccacaccgg gtcctgcggg gtgaaccacc gctgaccggg tttgccccag atcacggcag
    12541 cccgctgtgc cgcgtcatcg ccggcgaccc tggcgcgcag ggctgcgccg agtcgctgct
    12601 gagcgcgacg ggacaggtcg gtgacgggca gcatgaggct ccgatcggcg aggggacagg
    12661 gtccgcacga tccaacgtcg tgcgacccgg catccgcgcg cctgtctaac cgtgacgccc
    12721 gtcacacc
//
LOCUS       DAOSOC010000059        12506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5036_length_12506_cov_7.800257, whole genome shotgun sequence.
ACCESSION   DAOSOC010000059 DAOSOC010000000
VERSION     DAOSOC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5036_length_12506_cov_7.800257"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PL014_06260"
     CDS             <1..786
                     /locus_tag="PL014_06260"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOT56729.1"
                     /translation="GNFIFRTREFEQMEMEFFVKPGEDEEWHKYWIDERTRWYTDLGI
                     NPDNLRHYEHPVEKLSHYSKGTTDIEYRFNFTGSEWGELEGIANRTDFDLSTHTQHSG
                     TDLVYFDQASGEKYTPYVIEPAAGLSRSLMTFLVDAYTEDEAPNTKGGVDKRVVLKLD
                     PRLAPVKAAVLPLSRNADLSPKARDLAAMLRKNWNIEFDDAGAIGRRYRRQDEIGTPF
                     CLTVDFDTLDDQAVTIRERDTMAQERVALDQVEGWLAARLVGA"
     gene            complement(801..3593)
                     /locus_tag="PL014_06265"
     CDS             complement(801..3593)
                     /locus_tag="PL014_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56730.1"
                     /translation="MSQWDQVSEQFARLSLAADEAPIGMERVRAHEMALRLAQSNQSF
                     AEEFVARIDLTQALYYVPDDPHTLVHFTWLRRALDPVHELEEDDRHAVLWRLKWAIDL
                     IEALPEVPLSALVAAIDDVEEVYRAEGAALRPVYAARARLAQRLGDRDAVARELSAWL
                     AEPRDRHSDCLACEHRGQSRLVAPDDPARALDLLAPVIDGDLTCGEEPRTSLADAALL
                     RLHLGDVEGAVSAFRRSWHLAQDDPTAALNVGTCLRVLLRLGNTDRAIDLLLPRLGWL
                     DDLPSPWARMWFSATAAHVLDRAAAVGLAPDDIDGRPVADVVRDLTSTSDDIAAAFDA
                     RYSSTVVSRSLAAAHDPSLVPTEPTLPPTRLPTTAAPTGGRRSATRATASTDVLERAR
                     ALSEALLTLDPATEDHVAAWLRDRAALLPVETPEQWAAVGLLDRVSSVDAPGDEHRAM
                     LESAREASQRGGDDVGVIRCDGELALLDDDGAERAREIAAHLESIGAHTEAGGLWRRL
                     FHRPRGGELRDLERAADAYRAAQSRERALLCEVETAMALASTDPDPVAADARFSALMP
                     RIQDVPVLRSMALDARSRIANAAGDSDSALTHLRSALEGRGLPERARIPLLLGLCEVL
                     VEQNTYDELEEPAADLVAAATRLRDPVLLAHGQRFLGLAYVETGRPVEAAELLEAALP
                     VLRETSPSLVGPAAWALGNALIGLGQWAPGRTAFATASTAFEAEARIMESAHAQWRAG
                     NCAWDAGDLTAAAVHYESAAERARETATVDLYLEALRSRAALRADTEDLTAGIADLDA
                     AIGLAQVLADEAGVDDDEFDGEVMEPHVLRQGAHLLAKHGEVDAAVARFAAAQALVGA
                     EFEVLLRAEGAMVLADHDRLAEAEPVLREAITSLHALGLVDERVNAAGALARALDRDG
                     RGEEAEQVWQRYGPSA"
     gene            complement(3590..>4896)
                     /locus_tag="PL014_06270"
     CDS             complement(3590..>4896)
                     /locus_tag="PL014_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56731.1"
                     /translation="GTDVALRPRGSAADLLSAEVVERLASSYASYLPVDLVVETAGGP
                     VIAAGRRFPWEGGGRDAALSLGEVVVGARPLDVVDLSDPVSGVRGQAFVLPHPTGTRG
                     GHRLYAKRMLVGESVPDVLPDWAFFVRAVLDTERLALTASRESLADDDELAQTRARLG
                     DQLKRWLLRMARTDRARAQEFFGVHHLAVKAAATTDDDMLEVVAELLPWETTVGQMTL
                     AQFTAIDRVITYVDGVEDFQQVAAIARAEDIAVLNAGYAYDRTILERWVARTDGLEAV
                     RLEPEHLADRFSDPDPDEAAAFAPLLDVARQVLGRSEVRPVVRSFRPATMHAVLLVGR
                     DAHRERDRRDVAAAVDDPWSAALAEIAEPDAVARFVLNAEHHAVRRLADCGDAELQRV
                     ALEALYAHALLAGRHPLTPFDSALVARALPALIDRAVDGGPA"
     assembly_gap    4897..4996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4997..5402)
                     /locus_tag="PL014_06275"
     CDS             complement(<4997..5402)
                     /locus_tag="PL014_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT56732.1"
                     /translation="MTGATDSPFHVDLRGVVDLLSHHLYSGPRVYLRELLQNSLDAVT
                     ARTQDDPQAPRRVRIVPADLSPDGCLHVDDSGIGLDEDGIRAVLATIGASTKRDALGL
                     ARESFLGQFGIGLLSCFLVTDEIRVTTRRAGTD"
     gene            5444..7219
                     /locus_tag="PL014_06280"
     CDS             5444..7219
                     /locus_tag="PL014_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56733.1"
                     /translation="MDGSNGLSEAEAEFVAFAQSRQPELLRVAHLVCGDVGIAQGVLR
                     HVFVTLARQWGRVEEERPDAVVRHLVYRDAVASWRRRDLPSPRAGEHGIRWQDGGLDE
                     DEDAADDERRRAEVMAALDSLTPAQRAVIVACHLEDHGEDAAAPLLGRPLRVVRRDDE
                     AVLALLGWTPDDARENLELASDDLPETDLVGDAWAQAGRQRRSVVRRSLLGAGLALAG
                     GAVAAVALREPDAPPGPRPVPTSSSSAAVADGRLPLLQVQGVRVYLAPDPRSEANLPV
                     YPLREELALPETLDAGPVEARAVLGEQGLTGVANAVRGVFMVATSGDRSSPVLYAPNA
                     STPYVLVPSVQMPLSSLRSGPLGPRSIADDRHRVVFHRPAAVVILDARDGSVIEVPVP
                     DPTIAAAGWARDGRTVVARGRDAGWLIDPENYGVRRAENAVSPDWSDLVDSGGATVLR
                     TFSGEGELTGTKDLRGPPLVPHGTSVSNTEGWVSTGAWLPETYQRAVQRSQGLMAAQG
                     DLPPIPRVLAATEGPLVPAQCYRALGWGPSAVVFLESRSERAGFQGTVRRVLAWDVTR
                     ARLWRVADIGPASLMLGDFTGSFAI"
     gene            complement(7233..8063)
                     /locus_tag="PL014_06285"
     CDS             complement(7233..8063)
                     /locus_tag="PL014_06285"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOT56734.1"
                     /translation="MSALVTAADLSDDLSRRAVKVIDVQYNLRGTPGRELYAAAHLPG
                     APHLDLDAALAGPPGSGGRHPLPDPGVLEDALRSCGIDDDDEVVVYDQSTSLSAGRAW
                     WVLRWAGHPQVKVLDGGLAAWRQAGLPVSDAVPSPSRGDLTVRAGSVPVLDALGAADM
                     ARAGVLLDARTPERFRGESEPIDRVAGRIPGSTNLPIGDLLNTDGTFRSPEEIRRVAA
                     VAGVHRDTPVGTTCGSGVTAAQLALALHTAGIDAIPYVGSWSEWIEDPNRPIATGPTV
                     "
     gene            8113..8844
                     /locus_tag="PL014_06290"
     CDS             8113..8844
                     /locus_tag="PL014_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT56735.1"
                     /translation="MAGQVRVVLVHGSQLSAAQWTRYATLLGPDVDLAVPDLPAHGTR
                     RDEAFTWERALEVVGESVEGGVAGVPVVLVGHSLGGYLAMAYAAAHPQRLSGLGLLGS
                     SAVPAGPGAALYRSIASAEQRLGVDRMSRALDRQFAAMLPEELAAVIKEAGYGFDGIP
                     AAWAGVMGNCRPAMLGEVTCPVLLVNGQLDQLRVDVRRYARAAVSARWVRVVTVRHGL
                     HVFPLTHPHETAAALWELVSAARGS"
     gene            complement(8822..9601)
                     /locus_tag="PL014_06295"
     CDS             complement(8822..9601)
                     /locus_tag="PL014_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT56736.1"
                     /translation="MSPADGVRATWLGHASVLLDLGGVRLLTDPLLRPRLGPLKRRHD
                     LPVHRHVDDVDAVLLSHLHHDHADLPSLRRLGDIPVVTERSNVPWLDKNRLGIGDAAS
                     DDWHPIAPGVEVLLVRADHEARPMPHRPNGATGMLVRGGGVVVWFAGDTSLHPDMDLL
                     PQLAGAPIDLALLPVGGWGPRLSPGHMGPAEAAEAAVRSGARHVLPIHYGTLHPSGWP
                     TSRLAWTTDPGHRFADDLRAVSDAVAHVPAVGAAVTIRAPR"
     assembly_gap    9650..9749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9750..10742
                     /gene="dusB"
                     /locus_tag="PL014_06300"
     CDS             <9750..10742
                     /gene="dusB"
                     /locus_tag="PL014_06300"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT56737.1"
                     /translation="VERSTESMRLISHDPQEHPRSVQLYSVDPVTAGLAARMLVTEDR
                     ADHIDLNFGCPVPKVTRKGGGAALPWKTGLFRSIVAAVVREASPYSVPVTVKMRVGID
                     AEHITYLDAGRIAEAEGVAAVALHGRTASQAYSGQADWSAIARLKEAVTSIPVLGNGD
                     IWSAEDALEMVRQTGCDGVVVGRGCLGRPWLFADLAAAFAGSEVRVRPGLREVTATLR
                     RHTEYLVDFYDGDEQRACRDIRKHIAWYLKGFPAGSTVRHQLALVDSLQALDDLIGTM
                     DLDVPWPGDAAEGQRGRAGSPRVVALPENWLRSRELDEAQAAQVAQAELSVSGG"
     gene            10803..>11350
                     /locus_tag="PL014_06305"
     CDS             10803..>11350
                     /locus_tag="PL014_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOT56738.1"
                     /translation="MTDRIGTPGGTPERPALFFADAAEFGRWLERHHATETELWMGLY
                     RKAHPDAGLQWPEAVREALCWGWIDSMAQGIDEHTRRQRWTPRKKGSNWSAVNIKAVA
                     ELTEAGRMRPAGLAAFALRREDRSGVYTYETRELELTPAYRAMLEADVVANAFWGAAT
                     NGYRKLAVNWVMTAKQEKTRDSR"
     assembly_gap    11351..11450
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11451..>12506
                     /locus_tag="PL014_06310"
     CDS             <11451..>12506
                     /locus_tag="PL014_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOT56739.1"
                     /translation="LTHSLEVAQIGREFGAALGCNADVVDTACLAHDLGHPPFGHNGE
                     TALDAVAAGIGGFEGNAQTLRLLTRLEAKRTRPDGRSAGLNLTRASLDATTKYPWRRG
                     AGPRPTVKFGVYDDDLDVFEWFRDGVEPGRRSAEAEVMDWSDDVAYSVHDVEDAIASG
                     WLDPQRLRDPSDIEAVLAVAGEIYEPDLSADELGAALERLLATGAVPSTYDGSRPALA
                     ALKDMTSHLIGRFVTAVEQATRAEHGPQPLTRHTGRLVVPRGSRAECAVLKAVAAHFV
                     MHAAERVALYARQRDVIAGLVAAYESDPHRLDPDLRGDWEAAGDDGAALRVLVDQVAS
                     LTDVRALTLHRRWCRDT"
BASE COUNT         1668 a         4344 c         4569 g         1625 t
ORIGIN      
        1 ggcaacttca tcttccgcac ccgcgagttc gagcagatgg agatggagtt cttcgtcaag
       61 cccggcgagg acgaggagtg gcacaagtac tggatcgacg agcgcacccg ctggtacacc
      121 gacctcggca tcaaccccga caacctgcgc cactacgagc acccggtcga gaagctctcg
      181 cactactcca agggcaccac cgacatcgag taccgcttca acttcaccgg cagcgagtgg
      241 ggtgagctcg aggggatcgc gaaccgcacc gacttcgacc tcagcaccca cacgcagcac
      301 tcgggcaccg acctggtcta cttcgaccag gcgagtggcg agaagtacac gccgtacgtc
      361 atcgagccgg ccgccgggct ctcgcgcagc ctgatgacgt tcctcgtcga cgcgtacacc
      421 gaggacgagg cccccaacac caagggtggg gtcgacaagc gcgtcgtgct caagctcgac
      481 ccgcgcctgg ctccggtcaa ggccgccgtg ctgccgctgt cgcgcaacgc cgacctgtcg
      541 ccgaaggccc gcgacctcgc cgcgatgctg cgcaagaact ggaacatcga gttcgacgac
      601 gccggtgcca tcggccgccg ctaccgccgc caggacgaga tcggcacccc gttctgcctc
      661 accgtcgact tcgacaccct cgacgaccag gccgtgacga tccgcgagcg cgacaccatg
      721 gcgcaggagc gggtcgccct cgaccaggtc gagggctggc tcgcggcccg cctggtcggc
      781 gcctgatcag gctgcactga tcaggccgac gggccgtagc gctgccagac ctgctcggcc
      841 tcctcaccgc ggccgtcgcg gtccagcgcc cgggccagcg ccccggcggc gttgacccgc
      901 tcgtcgacca ggcccagtgc gtgcagcgac gtgatcgcct ctcgcagcac gggttcggcc
      961 tcggcgagcc ggtcgtggtc ggcgagcacc atcgcgccct cggcacgcag caacacctcg
     1021 aactcggcac cgacgagagc ctgggcggcc gcgaaacggg cgacggcggc atccacctcg
     1081 ccgtgcttgg cgagcaggtg tgcgccctgg cgcagcacgt gcggctccat gacctcaccg
     1141 tcgaactcgt cgtcgtccac gccggcctcg tcagcgagca cctgggccag cccgatcgcg
     1201 gcgtcgaggt cggcgatccc ggcggtgagg tcctcggtgt cggcgcgcag cgcagcgcgc
     1261 gagcgcagcg cctcgaggta gaggtcgacc gtcgcggtct cgcgggcgcg ctcggccgcg
     1321 ctctcgtagt gcaccgctgc cgcagtgagg tcgccggcat cccaggcgca gttgcccgcg
     1381 cgccactggg cgtgcgcgga ctccatgatc cgtgcctcgg cctcgaaggc ggtggatgcc
     1441 gtggcgaacg ccgtgcgtcc cggcgcccac tggccgagcc cgatgagagc gttgcccagg
     1501 gcccaggcag cagggccgac gagcgagggg ctggtctcgc gcaggacggg cagggctgcc
     1561 tccagcagtt cggccgcctc gaccggtcga ccggtctcga cataggccaa gccgaggaag
     1621 cgttgtccgt gggcgagcag cacggggtcg cgcaggcggg ttgctgcggc gaccaggtcg
     1681 gcggccggct cctccagctc gtcgtaggtg ttctgctcga cgaggacctc gcacagcccg
     1741 agcaggagcg ggatgcgggc gcgctcgggc aggccgcgcc cctccagggc cgagcgcagg
     1801 tgggtcaggg ccgagtcgct gtcaccggcg gcgttcgcga tccgactgcg cgcgtccagg
     1861 gccatcgagc gcaggaccgg gacgtcctgg atccgcggca tgagggcgct gaaccgggcg
     1921 tcagcggcaa ccgggtcagg gtcggtcgag gcgagcgcca tcgccgtctc gacctcgcag
     1981 agcagggcgc gctcgcggga ctgtgcggcg cggtaggcgt ccgcggcgcg ttcgaggtcg
     2041 cgcagctcgc cgcctcgcgg gcggtggaac agccgccgcc acaggccgcc ggcctcggtg
     2101 tgtgccccga tggactccag gtgggccgcg atctcacggg cccgttcggc cccgtcgtcg
     2161 tcgaggaggg cgagttcgcc gtcacagcga atcacgccga cgtcgtcgcc accacgctgc
     2221 gaggcctccc tcgcggactc gagcatcgcg cggtgctcgt caccgggggc gtccacggaa
     2281 ctgacccggt cgagcaggcc cacggcggcc cactgctcgg gggtctcgac cggtagcagg
     2341 gcggcccggt cgcgcagcca ggcggcgacg tggtcctccg tggccggatc gagggtcagc
     2401 agcgcctcgc tgagggcccg cgcccgctcg agcacgtctg tggatgccgt ggcacgcgtc
     2461 gcgctgcggc gcccgcccgt gggcgcggcc gtcgtgggaa ggcgtgtggg cggcaacgtc
     2521 ggctcggtcg gcaccagcga ggggtcatgg gcggccgcca gagaccgtga caccaccgtc
     2581 gaggagtagc gcgcgtcgaa ggccgcagcg atgtcgtccg acgtggaggt caggtcgcga
     2641 acgacgtcag cgacggggcg cccgtcgatg tcgtcagggg ccagacccac ggcggccgcg
     2701 cggtcgagca cgtgtgcagc ggtggcggag aaccacatcc gggcccacgg ggaggggagg
     2761 tcgtcgagcc agccgaggcg cgggagcagg aggtcgatcg cgcggtcggt gttgcccagg
     2821 cgcagcagca cccgcaggca ggtgccgacg ttcagggcgg cggtggggtc gtcctgggcg
     2881 aggtgccacg accggcggaa ggcgctgacg gcgccttcga cgtcgccgag gtgcaggcgc
     2941 agcagcgcgg cgtcagcgag gctggtgcgc ggctcctcgc cgcaggtgag gtcaccgtcg
     3001 atcaccgggg cgagcaggtc gagggcccgg gccgggtcat cgggggcgac cagccggctc
     3061 tgcccacggt gctcgcaggc caggcagtcg ctgtgcctgt cccgcggctc ggcgagccac
     3121 gcactgagct cgcgggcgac ggcatcccgg tcgccgaggc gctgcgccag gcgggcgcga
     3181 gcggcgtaca cgggacgaag cgccgcaccc tcggcgcggt agacctcctc gacgtcgtcg
     3241 atggccgcca ccaaggccga caacggcacc tcggggaggg cctcgatgag gtcgatcgcc
     3301 cacttcaggc gccacagcac cgcgtggcgg tcgtcctcct ccagctcgtg gacggggtcc
     3361 agggcccgtc gcagccacgt gaagtggacg agggtgtgtg ggtcgtcggg cacgtagtac
     3421 agggcttggg tgaggtcgat gcgggcgacg aactcctcgg cgaacgactg gttggactga
     3481 gccaggcgca gcgccatctc gtgggcgcgc acacgctcca tcccgatggg tgcctcgtcc
     3541 gccgccagcg acagcctggc gaactgctct gacacctgat cccactggct catgctggtc
     3601 ctccgtcgac ggcccggtcg atcagtgccg gcagcgctcg ggccacgagg gcgctgtcga
     3661 acggggtgag ggggtgccgc ccggcgagca gggcgtgggc gtacaacgct tcgagggcga
     3721 cccgttgcag ctcggcgtca ccacagtccg cgaggcggcg caccgcgtgg tgctcggcgt
     3781 tgaggacgaa gcgcgccacg gcatccggct cggcgatctc ggcgagggcg gccgaccagg
     3841 ggtcgtcgac cgcggcagcc acgtcacgtc ggtcgcgttc gcggtgcgca tcgcggccga
     3901 ccaacaggac ggcgtgcatc gtggcggggc ggaacgagcg caccaccggc ctcacctcgg
     3961 atcgcccgag cacctggcgg gcgacgtcca gcagcggcgc gaaggcggcg gcctcgtcgg
     4021 gatcgggatc gctgaaccgg tcggcgaggt gttcgggttc caggcgcacg gcctccaacc
     4081 cgtcggtgcg ggcgacccat cgttcgagga tggtgcggtc gtaggcgtac ccggcgttga
     4141 gcaccgcgat gtcctcggcc cgtgcgatgg cggccacctg ctggaagtcc tcgaccccgt
     4201 cgacgtaggt gatcacccga tcgatcgccg tgaactgcgc caaggtcatc tggccgaccg
     4261 tggtctccca cggcagcagc tcggcgacga cctccagcat gtcgtcgtca gtcgtcgcgg
     4321 cggccttgac ggccaggtgg tgcaccccga agaactcctg cgcgcgggcc cggtcggtgc
     4381 gggccatccg cagcagccag cgcttgagct ggtcgccgag ccgggcgcgg gtctgcgcca
     4441 gctcgtcgtc gtccgccagc gactcgcgcg aggcggtcag ggcaagccgc tcggtgtcga
     4501 gcacggcccg cacgaagaac gcccagtcgg gcagcacgtc cggcaccgac tcgccgacga
     4561 gcatgcgctt ggcgtagagc cggtgcccac cgcgcgtgcc ggtggggtgc ggcaggacga
     4621 aggcctggcc ccgaaccccc gacacggggt cggagaggtc cacgacgtcc agtggccggg
     4681 ccccgaccac cacctcgccg agggagagcg cggcatcccg cccgcccccc tcccacggga
     4741 agcgccgtcc tgctgcgatc accggcccgc ccgcggtctc gacgacgaga tccaccggca
     4801 ggtaggaggc gtaggacgac gcgagccgtt cgacgacctc ggcgctgagc aggtcggctg
     4861 cgctccctcg gggtcgcagg gccacgtcgg tgccggnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnncgtc ggtgccggcc cgccgcgtcg tcacccggat ctcgtcggtg
     5041 acgaggaagc acgacaacag cccgatgccg aactgaccga ggaagctctc gcgcgccaac
     5101 cccagggcgt cgcgtttggt ggaggcgccg atggtcgcga gcaccgcccg gatgccgtcc
     5161 tcgtcgagcc cgataccgga gtcgtcgacg tgcaggcagc cgtccgggga gaggtccgct
     5221 ggcacgatgc gcacccggcg cggggcctgc ggatcgtcct gcgtgcgggc ggtcacggcg
     5281 tcgagcgagt tctggaggag ctcacgaagg tagacccggg gcccggagta caggtggtgc
     5341 gacaacaggt cgaccacgcc tcgcaggtcg acgtggaatg gtgagtcagt cgcccccgtc
     5401 acgggacgat ctaagcagac cgaacgcgag gaggtggtgg ggcgtggacg ggtcgaacgg
     5461 gctgtccgag gccgaggccg agttcgtcgc gttcgcgcag tcgcgccaac ccgagctcct
     5521 gcgggtggcc cacctcgtgt gtggtgacgt cgggatcgcg cagggggtgc tgcgccacgt
     5581 cttcgtgacg ctcgcgcggc agtggggccg ggtcgaggag gaacgaccgg atgccgtggt
     5641 gcgccacctg gtgtaccgcg acgccgtcgc gtcgtggcgg cgtcgggacc tgccgtcccc
     5701 gcgggccggt gagcacggca tccggtggca ggacggtggc ctcgacgagg acgaggacgc
     5761 agccgacgac gaacgccgtc gcgccgaggt catggcggca ctggactcgc tcaccccggc
     5821 ccaacgggcc gtcatcgttg cctgccacct cgaggaccac ggtgaggacg ctgctgcccc
     5881 cctgctcggt cggccgctgc gggtggtgcg ccgcgacgac gaggccgtcc tcgcgctgct
     5941 cgggtggacg ccggatgacg cccgcgagaa cctcgagctg gccagcgacg acctgcccga
     6001 gaccgacctc gtcggtgacg cctgggcgca ggccgggcgg cagcggcgct cggtggtgcg
     6061 gcgcagcctg ctcggcgcag gccttgccct cgccggcggc gcagtggccg ccgtggcgct
     6121 gcgcgagccc gatgcaccac cggggccacg cccggtgccc acgtcgtcgt cgtccgcggc
     6181 ggtcgccgac ggtcggctgc cgctgctcca ggtccagggc gtcagggtct acctcgcgcc
     6241 ggaccctcgc tcagaggcca acctgccggt gtaccccctg cgcgaagagc tcgcgctccc
     6301 cgagaccttg gacgccggtc cggtggaggc gcgagcggtt ctgggcgagc agggcctgac
     6361 cggggtggcc aacgccgttc ggggtgtgtt catggtggcg acctcgggcg atcgctcgtc
     6421 gccggtgctc tacgccccca acgcgtcgac accgtacgtt ctcgtgccgt cggtgcagat
     6481 gccgttgtcg tccctgcgct caggaccgct cggcccacgc tcgatcgccg acgaccgaca
     6541 ccgggtggtg ttccaccgac cggccgcggt cgtcatcctc gatgcccggg acggctccgt
     6601 catcgaggtg ccggtgcccg acccgaccat cgccgcggcc ggctgggcgc gtgacgggcg
     6661 caccgtcgtg gcccgtgggc gcgatgccgg atggctcatc gaccccgaga actacggcgt
     6721 ccggcgtgcc gagaacgccg tgagccccga ctggtcagac ctggtggact ccggcggcgc
     6781 caccgtgctg cgcaccttct ccggtgaggg ggagctgacc ggcaccaagg acctgcgcgg
     6841 cccaccgctc gtgccgcacg gcacgtcggt ctccaacacc gagggctggg tcagcacggg
     6901 cgcgtggctt ccggagacct accagcgtgc ggtccagcga tcgcaggggc tgatggccgc
     6961 ccagggggac ctgcccccga tcccgcgggt gctggccgcg acagagggcc cactcgtccc
     7021 ggcccagtgc taccgagccc tcggatgggg cccgtccgcc gtggtgttcc tcgagtcccg
     7081 gtccgagcga gcggggttcc agggaacggt gcgccgggtg ctcgcctggg acgtcacccg
     7141 ggcccgactg tggcgggtgg ccgacatcgg accggccagt ctcatgctcg gcgacttcac
     7201 cgggtccttc gcgatctgag tgctgtcagg cctcagaccg tcgggccggt cgcgatcggc
     7261 cggttcgggt cctcgatcca ctcgctccac gagccgacgt aggggatcgc gtcgatcccg
     7321 gccgtgtgca gcgccagagc cagctgcgct gcggtgacgc ctgaaccgca cgtcgtgccg
     7381 accggggtgt cgcggtgcac ccccgccacg gccgccaccc ggcggatctc ctccggcgag
     7441 cggaacgtgc cgtcggtgtt cagcaggtcg ccgatgggca ggttcgtcga cccggggatg
     7501 cgtccggcga cgcggtcgat gggctcgctc tcaccgcgga accgctccgg ggtgcgggcg
     7561 tcgagcagca cgccggcgcg ggccatgtct gcggcgccga gtgcatcgag gacggggacc
     7621 gagcccgcgc gcacggtgag gtcaccgcgc gacggcgacg gcacggcatc cgagacgggc
     7681 aagccggcct gccgccacgc ggccaggccg ccgtcgagca ccttcacctg cgggtggccc
     7741 gcccagcgca gcacccacca ggcccgaccc gcggacaacg aggtgctctg gtcgtagacg
     7801 acgacctcgt cgtcgtcgtc gatcccgcag gagcgcagcg cgtcctcgag cacccccggg
     7861 tcggggagcg gatgccgccc gcctgacccc ggtgggccgg cgagggccgc gtcgaggtcg
     7921 aggtggggtg cacccggaag gtgcgccgcg gcgtacagct cgcggcccgg ggtcccccgc
     7981 aggttgtact gcacgtcgat gaccttcacg gcccgtcggg acaggtcgtc ggaaaggtca
     8041 gcggcggtca ccagggcgct catcccgcca tccaagcacc gatcggatgc cgcgctgggg
     8101 gaggatggtc gggtggctgg tcaggtgcgt gtcgtcctcg tccacggttc ccagctgagc
     8161 gcggcgcagt ggacccgcta cgccaccctg ctcgggcccg atgtcgacct cgccgtgccc
     8221 gacctgccgg cacacggcac gcgtcgggac gaggcgttca cctgggaacg cgcgctcgag
     8281 gtcgtggggg agtccgtcga gggtggcgtt gccggcgtgc ccgtcgtcct cgtggggcac
     8341 agcctcgggg gttacctggc gatggcgtat gccgcggccc acccgcagcg cctgtccggg
     8401 ctcggcctgc tcgggtcgtc ggcggtaccg gcggggccgg gggccgcctt gtaccggagc
     8461 atcgcctcgg ccgagcagcg cctgggggtc gaccggatgt cgcgggcgct ggaccggcag
     8521 ttcgccgcga tgctgcccga ggaactggcg gccgtcatca aggaggccgg ctacggcttc
     8581 gacggcatcc cggcggcatg ggccggtgtg atgggcaact gccggccggc gatgctcggc
     8641 gaggtgacct gcccggtgct gctcgtcaac ggccagctcg accagcttcg cgtcgacgtc
     8701 cggcgctacg cgcgggccgc ggtcagcgcc cggtgggtgc gcgtggtcac cgtgcgccac
     8761 gggctgcacg tcttcccgct gacccacccg cacgagacgg cggcagcact gtgggagctg
     8821 gtcagcgcgg cgcgcggatc gtgacggcgg cgccgaccgc cgggacgtgc gcgacggcat
     8881 ccgagacggc ccgcaggtcg tcggcgaagc ggtggccggg gtcggtggtc caggccaggc
     8941 gtgaggtcgg ccagccggag gggtgcagcg tgccgtagtg gatcggcagc acgtggcggg
     9001 caccgctgcg cacggctgcc tcggccgcct ctgccggtcc catgtgaccg ggggagagcc
     9061 gcggtcccca cccgccgacg gggagcaggg ccaggtcgat cggggcgccg gcgagctggg
     9121 ggagcaggtc catgtcgggg tgcaacgagg tgtcaccggc gaaccacacg acgaccccgc
     9181 cgccacgcac gagcatcccg gtggcgccgt tcggccggtg cggcatggga cgggcctcgt
     9241 ggtcggccct gacgaggagc acctcgaccc cgggggcgat ggggtgccag tcatcgctgg
     9301 cggcatcccc gatccccagc cggttcttgt ccagccaggg cacgttgctc cgctcggtga
     9361 ccaccgggat gtcgcccagc cgccgcagcg agggcaggtc ggcgtggtcg tggtgcaggt
     9421 ggctgagcag cacggcgtcg acgtcgtcga cgtggcggtg cacgggcagg tcgtgccggc
     9481 gcttcagcgg tcccagacgc ggccgcagca gcggatcggt gagcaggcgg accccgccga
     9541 ggtcgagcag cacgctcgcg tgcccgagcc aggtggctcg cactccgtcg gctgggctca
     9601 cccgatccac tctagagggc gctgagccca cccccgcgtg agcgcgctcn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tcgagcgcag cacggagtcg atgcggctga
     9781 tctcccacga cccgcaggag cacccgcggt cggtgcagct gtactccgtc gacccggtga
     9841 ccgcggggct ggctgcccgc atgctcgtca ccgaggaccg ggccgaccac atcgacctca
     9901 acttcgggtg cccggtgccc aaggtgaccc gcaagggtgg cggggcggcc ctgccgtgga
     9961 agaccgggct gttccggtcg atcgtcgcgg ccgtcgtgcg tgaggcgtcg ccgtactcgg
    10021 tgccggtcac cgtgaagatg agggtcggga tcgacgccga gcacatcacc tacctcgacg
    10081 ccgggcggat cgccgaggcc gagggggtgg cggcggtcgc cctccacggg cggacggcat
    10141 cccaggcgta ctcggggcag gccgactggt cggcgatcgc ccgcctcaag gaggccgtga
    10201 cctcgatccc cgtgctcggc aacggcgaca tctggtcggc ggaggacgcc ctcgagatgg
    10261 tgcgccagac cgggtgcgac ggggtggtcg tgggccgcgg atgcctgggg cggccatggc
    10321 tgttcgccga cctcgcggct gccttcgccg ggtccgaggt ccgggtgcgc cccggcctgc
    10381 gcgaggtgac agccaccctg cgacgccaca ccgagtacct cgtcgacttc tacgacggcg
    10441 acgagcagcg ggcctgccgc gacatccgca agcacatcgc ctggtacctc aaggggttcc
    10501 cggcgggatc gaccgtgcgc caccagttgg ctctcgtcga cagcctccag gcgctcgacg
    10561 acctcatcgg caccatggac ctcgacgtcc cctggcccgg ggatgcagcc gagggccagc
    10621 ggggtcgggc cgggtcgccg cgggtggtgg ccctgccgga gaactggctg cgctcgcgcg
    10681 agctcgacga ggcccaggcc gctcaggtgg cgcaggccga gctgagcgtc tccggcggct
    10741 gagccctcgg tggggcggca tcgggccggt ggccgtcagg agggatcgct atcgtcgcgc
    10801 gcatgactga tcggatcggg acaccggggg gcacgcccga gcggccggcc ctgttcttcg
    10861 ccgatgccgc ggagttcggc cggtggctgg agcggcacca cgccaccgag accgagctgt
    10921 ggatggggct gtaccgcaag gcgcacccgg atgccgggct gcagtggccc gaggcggtgc
    10981 gtgaggcgct gtgctggggc tggatcgaca gcatggcgca gggcatcgac gagcacaccc
    11041 gccgccagcg ctggacgccg cgcaagaagg gcagcaactg gtcggccgtc aacatcaagg
    11101 ccgtcgccga actcaccgag gccggccgga tgcggccggc ggggctcgcg gcgttcgccc
    11161 tgcgccgaga ggaccgctcc ggcgtctaca cctacgagac gcgcgagctc gagctgactc
    11221 cggcctaccg ggcgatgctc gaggccgacg tggtggcgaa cgctttctgg ggtgcggcca
    11281 ccaacggcta ccgcaagctc gccgtcaact gggtcatgac cgccaagcag gagaagaccc
    11341 gcgactcgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggttgacgca
    11461 ctcgctcgag gtcgcccaga tcggccgcga gttcggcgcc gctctcggct gcaacgccga
    11521 cgtcgtcgac accgcgtgcc tggcccacga cctcgggcac cccccgttcg ggcacaacgg
    11581 cgaaacggcg ttggatgccg tggcagccgg gatcggcggt ttcgagggca acgcgcagac
    11641 cctgcggctg ctcactcgcc tcgaggccaa gcgcacccgg cccgacggtc gctccgccgg
    11701 gctgaacctc acgcgagcca gcctcgacgc cacgacgaag tacccgtggc ggcggggcgc
    11761 gggcccgagg cccaccgtca agttcggggt ctacgacgac gatctcgacg tcttcgagtg
    11821 gttccgcgat ggtgtggagc ccggtcgccg gtccgccgag gccgaggtga tggactggtc
    11881 cgacgacgtg gcgtactccg tgcacgacgt cgaggacgcc atcgcctccg ggtggctcga
    11941 cccacagagg ctgcgcgacc ccagcgacat cgaggcggtg ctggccgtcg cgggcgagat
    12001 ctacgagccc gacctgtcgg cggacgaact cggtgcggcc ctggaacgcc tcctcgccac
    12061 cggcgccgtg ccctccacct acgacgggtc acgcccggcc ctggcagccc tgaaggacat
    12121 gacctcacac ctcatcgggc ggttcgtgac cgccgtcgag caggccaccc gggctgagca
    12181 cggcccgcag ccactgaccc gccacaccgg ccggctcgtc gtgccccgcg gttctcgggc
    12241 cgagtgcgcg gttctcaagg ccgtggcggc acacttcgtc atgcacgccg ctgagcgggt
    12301 ggccctctac gcccgtcagc gtgacgtcat cgcgggtctg gttgccgcgt acgaaagcga
    12361 tccccaccgg ctcgatccgg accttcgcgg ggactgggag gctgcgggcg atgacggggc
    12421 cgccctgcga gtcctcgtcg accaagtcgc ctcgctcact gacgtccggg cgctcaccct
    12481 gcaccgcagg tggtgtcgcg acacgc
//
LOCUS       DAOSOC010000060        12487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5046_length_12487_cov_10.246219, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000060 DAOSOC010000000
VERSION     DAOSOC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5046_length_12487_cov_10.246219"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1235)
                     /gene="ychF"
                     /locus_tag="PL014_06315"
     CDS             complement(150..1235)
                     /gene="ychF"
                     /locus_tag="PL014_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOT56740.1"
                     /translation="MALTIGIVGLPNVGKSTMFNALTKNNVLAANYPFATIDPNIGVV
                     PLPDDRLNRLAEIFGSEKILPATVSFVDIAGIVRGASEGEGLGNKFLANIREADAICQ
                     VVRAFEDGDVVHVDGKVSPSSDIETIHTELILADLQTLEKAVARLEKESRIKKESRPA
                     FENAVAAQKVLEAGETLFAAGEKAGVDLDLAAGLGLLTTKPFIYVFNLDEDQLADADL
                     HASLAELVAPADAVFLNAKLEMDLMEMSAEDALEMLQSFGAEESGLDQLARVGFHTLG
                     LQTYLTAGPKESRAWTIHQGWTAPQAAGVIHTDFQRGFIKAEIVSFADLDAAGSMAAA
                     RAAGRVRIEGKEYVMADGDVVEYRFNV"
     gene            complement(1393..1899)
                     /locus_tag="PL014_06320"
     CDS             complement(1393..1899)
                     /locus_tag="PL014_06320"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT56741.1"
                     /translation="MARSETEAERLKRIAILNARLPRTRAIAQGVLRDPKGRVLLCEL
                     SYKREWDLPGGVVDPGESPATCVEREIFEELGLTVKVGRLLAVNWLPPWRGWDDALLY
                     LFDLGVHHAGELDPALFLRREIAGAHWRSIADAAAHVAPYTARMLEVAADAEHPLYLE
                     NSEPGRTR"
     gene            complement(1909..2916)
                     /locus_tag="PL014_06325"
     CDS             complement(1909..2916)
                     /locus_tag="PL014_06325"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT56742.1"
                     /translation="MSEIRTSGDAALAARAAHHLGRHHDRFVVARVLRGQVHIAQVGL
                     ETGAQVEIGSVSKAVTGLLFDDSRRRGETSDDTPLGALLPVAGTPVAQVTLGELATHR
                     SGLPRLAKGTASVRTTLDLWRHGTNPYGETLDELLVAARATPVGRKRPSYSNLGFMLL
                     GHAVAAAAGTTYADLLTQRIAEPLGLVTTTVPATAAELRPAAVMGTSRRGKVMAPWVG
                     EALGPAGGIRSTPGDLGRLVQTLLDGSAPGTGALEPVQRLSGGVRIGAAWLVLEHRGA
                     VVTWHNGGTGGFRSILALNRDAGAGVALVSATARSVDSAGFGLLAECVAGADGSVAAD
                     G"
     gene            2969..4174
                     /locus_tag="PL014_06330"
     CDS             2969..4174
                     /locus_tag="PL014_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOT56743.1"
                     /translation="MNTWVWGLLGLAVGVAATIVVTRLVDAARSAAVVAERDLLRERV
                     LDLETSLAEDLETAALLAPLKDALVRVEHQVGTLERDRMQQFGAIRGLLARVEGETQH
                     LGRQTASLAGSLRSSTVRGAWGEVQLRRVLEASGMLARCDFDEQVTAVSAHDRGVRPD
                     VVVRLPGDKVLVVDAKAPMAAFLDAQGEDLTDDERGDLLREHAASLGRHVSALATKEY
                     WSAFREGPEMVVCFVPSDAMLGAALAAQPSLHDQALSQRVVLVGPGSLMALLRTVAFT
                     WQQDALSSSARELLDLGRDLHRRLATLGTHTAKVGRSLQSSVEAYNAMVGALESRVMV
                     TARRMNDLDLVDDELPVLTPVESAPRPLTAQELIDDVTAQDSRPELLLDLDVGEPARP
                     RAHGIREVG"
     gene            complement(4220..>5986)
                     /locus_tag="PL014_06335"
     CDS             complement(4220..>5986)
                     /locus_tag="PL014_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT56744.1"
                     /translation="ALTVPGYSMDGWYGRLFEGMGLPMDTPIADFTAKQIDTMLFAPP
                     TKIKVEGVNLTFEGIIPKIQKSMLSKDPEAMQPHVRRFVERAVTFQACPECSGTRLTA
                     EARASLINGKSIADLCAMQISDLAQWVRDLDEPSVAPLLTGLQHLLDSFTEIGLGYLS
                     LDRPAGTLSGGEAQRTKMIRHLGSSLTDVTYVFDEPTVGLHPHDIERMNTLLLQLRDK
                     GNTVLVVEHKPETIAIADHVVDLGPGAGSGGGTICFEGSVDGLRASDTITGRHLDDRA
                     TLKPSVRAATGAVEVRGASTHNLRGVDVDIPLGVLCVVTGVAGSGKSSLIHGSVAKRD
                     DVVVVDQGAIKGSRRSNPATYTGLLEPIRKAFAKVNGVKPALFSSNSEGACPTCNGAG
                     VIITDLGIMATMESPCEDCEGKRFQASVLEYTLGGKDIAEVLALSVDEAEVFFASGEA
                     RTPAAHTILARLADVGLGYISLGQPLSTLSGGERQRLKLAVQMADKGEIYVLDEPTTG
                     LHLADVGNLLGLLDRLVDSGKSVIVIEHHQAVMAHADWIIDLGPGAGHDGGTIVFEGA
                     PSDLVAARSTLTGQHLAQYVGA"
     assembly_gap    5987..6086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6087..6314)
                     /locus_tag="PL014_06340"
     CDS             complement(<6087..6314)
                     /locus_tag="PL014_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56745.1"
                     /translation="MTTPHPADSHDLIRVLGARENNLKGVNVELPKRRLTVFTGVSGS
                     GKSSLVFATIAAESKRMINETYSAFLQGFMPS"
     gene            complement(6348..7364)
                     /locus_tag="PL014_06345"
     CDS             complement(6348..7364)
                     /locus_tag="PL014_06345"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOT56746.1"
                     /translation="MADSGKRVLLAAPRGYCAGVDRAVVTVEKALDLYGSPVYVRKEI
                     VHNKHVVTTLQKRGAIFVDETDEVPEGATVVFSAHGVAPVVHEEARALSLRTIDATCP
                     LVTKVHREAVRFADEDYDILLIGHEGHEEVIGTAGEAPEHIQLVDGPDDVDNVTVRDP
                     DKVVWLSQTTLSVDETMETVRRLRERFPSLQDPPSDDICYATQNRQLAVKQMAADCDL
                     MLVIGSRNSSNSVRLVEVALEHGTPAGHLVDYADEIDESWLDGVSTVGVTSGASVPEI
                     LVREVLEYLEARGYGDVSPVVAAEESIMFSLPAEIRRDLKAAGKADTMRHDASFADST
                     SGLH"
     gene            7495..8730
                     /gene="xseA"
                     /locus_tag="PL014_06350"
     CDS             7495..8730
                     /gene="xseA"
                     /locus_tag="PL014_06350"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT56747.1"
                     /translation="MTTLPERAADTTAEQPWPVRLLSMKIGEYVEKMSTLWVEGQVVQ
                     LNRRPGARTAFLTLRDPDVDMSLSVSIHVNALDAMPTPLGEGARVVLQAKPTFWAQRG
                     SLMLDARQIRPVGVGELLARLEYLKRHLAQEGLFDRERKKPLPFLPRRIGLVCGRASA
                     AERDVVENARRRWPSAVFEIRQVAVQGTDAVPQVCAALAELDAHPEVDVIVIARGGGG
                     VEDLLPFSNETMVRAVAAALTPVVSAIGHDVDTPLLDLVADHRASTPTDAAKAIVPDA
                     AAERSAVAAARARGHRALTARLHHERRRLVELRSRPVLTDKGAFVRSQRSVVDALRDR
                     SRRRVEAQLHRARDHAAHLRTQVRTLSPQSTLDRGYAIVQHADGAVVSDPASVVVGEL
                     LRVRVARGDFGVKPVGHDG"
     gene            8741..8989
                     /locus_tag="PL014_06355"
     CDS             8741..8989
                     /locus_tag="PL014_06355"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT56748.1"
                     /translation="MATTADSATPEQGPAPDGAQHADIAGLTYEQARDELVDIVARLE
                     NGQSGLEESMGLWQRGEALAAHCATWLDRAEAKITVQE"
     gene            complement(9005..9562)
                     /locus_tag="PL014_06360"
     CDS             complement(9005..9562)
                     /locus_tag="PL014_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HOT56749.1"
                     /translation="MTTPAPARSRYSMGSAKNLVYSLAAVLGMVAVLVLIVPRVSSVS
                     GPPVDVHATAVDVQERTGWPIVEPVGLPEGWTATSARYTVTTGGFSTWHAGYQTPSGT
                     YVAVEQTLDPNREWIASQTNRAPKVGTLEAAGRTWTMYERDTKVQNSLVDAPEGEGVL
                     TTLITGTATFDEMKQFVETLQPVTP"
     gene            9671..10618
                     /locus_tag="PL014_06365"
     CDS             9671..10618
                     /locus_tag="PL014_06365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOT56750.1"
                     /translation="MNPSTADRAPGRAVAVIGELLVDIVHTPDGNTAEHVGGSPANVA
                     LGLSRLGHETWFATLVGTDDRGIRCAEHVESGGVHLLPGSISASHPTSTAAATIDESG
                     AASYVFDLHWDLAPVDLPADAGHLHIGSIGTTLRPGDEEVTAAVHAIRATGTVSYDPN
                     VRPSIMGSPDEVRPRIEELVALSDVVKCSEDDIAWLYPGQSPAEVMAHWAGLGSTLTL
                     VTLGGDGVTWRAGTGDVDHLPALADDVVDTVGAGDSFMAGLVSGLLDAGLLGDTAARE
                     RLQHAGLPDVRPAIDRALATSGITVTHAGAYAPTREELA"
     gene            10615..12333
                     /locus_tag="PL014_06370"
     CDS             10615..12333
                     /locus_tag="PL014_06370"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOT56751.1"
                     /translation="MTDFAYEDLLPIGDDETPYRLLTTDGVSVVDGPGGRSFLQVEPE
                     ALRLLTDTAMHDIAHYLRPAHLQQLANILDDPEASNNDKFVALDLLKNANIAAGGILP
                     MCQDTGTAIVMGKRGQHVLTPGRDEEAVSRGVYDAYTRLNLRYSQMAPVTTWDERNTG
                     SNLPAQIELYADTAPGHESTYKFLFMAKGGGSANKSFLFQETKAVLNPDAMMRFLDEK
                     LRSLGTAACPPYHLAIVIGGTSAEFALKTAKYASAKYLDQLPKAGDAATGHGFRDTEL
                     EEKVLELTRQFGIGAQFGGKYFCHDVRVVRLPRHGASLPIAIAVSCSADRQVLGKITP
                     EGVFIEQLETDPARFLPDQTHAALAGEDDVDAVGAASTGAGAVVSIDLNQPMADILAE
                     LTKHPVKTRLSLTGPLVVARDIAHAKIKERLDAGEPMPDYLRNHPVYYAGPAKTPEGM
                     PSGSFGPTTAGRMDSYVDQFQAAGGSMVMLAKGNRSQQVTDACKAHGGFYLGSIGGPA
                     ARLAQDCIRSVEVLEYPELGMEAIWKIEVEDFPAFIVVDDKGHDFFASVTKPVAFTIE
                     KRTGLL"
     gene            12333..>12487
                     /locus_tag="PL014_06375"
     CDS             12333..>12487
                     /locus_tag="PL014_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOT56752.1"
                     /translation="MPSTFDDLLEANRAYAAENPILGFDGVAHAGVAVVTCMDSRIDP
                     LRMIGLKP"
BASE COUNT         1869 a         4425 c         4270 g         1823 t
ORIGIN      
        1 gggtcacgtc gtgggtggat cacatggccg caggggtctg gacataggcc ctcagactgg
       61 cagatcgagc acatcgcgct gaacttcttg cggactgtgt gcggactggg agaagtcgca
      121 cccactctga cctgcgcaaa cgtgccgatt cagacgttga agcggtactc cacgacgtct
      181 ccgtcagcca tgacgtactc cttgccctcg atgcgcaccc gcccagccgc gcgagcagcg
      241 gccatcgagc cggcggcatc cagatcggcg aacgagacga tctcggcctt gatgaagccg
      301 cgctggaagt cggtgtggat gacaccggca gcctgggggg cggtccaacc ctggtgaatc
      361 gtccaggccc gcgactcctt ggggccggcc gtcaggtagg tctgcagccc gagggtgtgg
      421 aacccgacac gagcgagctg gtcgagaccg gactcctccg ccccgaagga ctggagcatc
      481 tcgagggcgt cctcggcgga catctccatg aggtccatct cgagcttggc gttgaggaac
      541 acggcgtcgg ccggcgcgac gagctcggcc aggctggcat gcaggtcggc gtcggcgagc
      601 tggtcctcgt cgaggttgaa gacgtagatg aacggcttgg tggtcagcag cccgagcccg
      661 gcagcgaggt cgaggtcgac gccggccttc tcaccggcgg cgaacagcgt ctcgcccgcc
      721 tcgagcacct tctgcgcggc gacggcgttc tcgaacgccg gacgcgactc cttcttgatc
      781 cggctctcct tctcgaggcg ggccaccgcc ttctcgaggg tctggaggtc ggcgaggatc
      841 agctcggtgt ggatggtctc gatgtccgag ctcggcgaga ccttgccgtc gacgtggacg
      901 acgtcgccgt cctcgaaggc gcgcacgacc tggcagatgg cgtcggcctc acggatgttg
      961 gcgaggaact tgttgccgag cccctccccc tcggaggcac cacgcacgat gccggcgatg
     1021 tcgacgaacg acaccgtcgc gggcaggatc ttctccgacc cgaagatctc ggcgagtcgg
     1081 ttcagccgat catcgggcag cgggacgacc ccgatgttcg ggtcgatggt cgcgaacggg
     1141 tagttcgcgg cgaggacgtt gttcttcgtc agcgcgttga acatcgtcga cttgccgacg
     1201 ttggggagac cgacgattcc gatggtgagt gccacgagga caggagttta gccgggttgg
     1261 gcgcctcccc ggtccacggc gtcggtcacc cggcgaaggc ggagaccacg atccagccga
     1321 ggatgccggc actcaccagc acgaccgcgc cgaggaacgc gagcgccacg cggctctcgt
     1381 cgtcggggat cttcatctgg tcctcccggg ttcgctgttc tcgaggtaga gcggatgttc
     1441 ggcgtcagcg gccacctcca gcatccgtgc ggtgtacggc gcgacgtgcg ccgcggcatc
     1501 cgcgatgctc cgccagtggg cgcccgcgat ctcgcgccgc aggaacagtg ccgggtcgag
     1561 ctcaccggcg tggtggaccc ccaggtcgaa caggtacagg agcgcgtcgt cccaccctcg
     1621 ccacggcggg agccagttga cggcgagcag tcgcccgacc ttcacggtga gccccagctc
     1681 ctcgaagatc tcacgctcga cgcaggtcgc cggcgactcc cccgggtcga cgaccccacc
     1741 gggcaggtcc cactcccgct tgtagctgag ctcgcagagc agcacccggc ccttcgggtc
     1801 gcgcagcacc ccctgggcga tggcccgggt ccgcggaagg cgggcgttga ggatcgcgat
     1861 gcgcttcagg cgctcggcct cggtctcact gcgtgccatc acccgaccct aaccgtcagc
     1921 cgcgacactg ccgtcggcac ccgccacgca ctcggccagc agtccgaagc ccgcagagtc
     1981 cacggagcgc gccgtggcgc tcaccagcgc gaccccggca ccggcatccc ggttcagggc
     2041 gaggatgctg cggaacccgc cggtcccacc gttgtgccac gtcacgacgg ccccgcggtg
     2101 ctcgagcacc agccacgccg cgccgattcg caccccgccg gagagccgct gcaccggctc
     2161 gagggcgccc gtcccgggag ccgagccgtc gagcagcgtc tggaccagac gaccgagatc
     2221 gccgggcgtg gaccggatgc cgcccgctgg gccgagtgcc tcgcccaccc acggcgccat
     2281 caccttgcct cggcgtgagg tgcccatgac ggcggccgga cgcaactcgg cagccgtggc
     2341 gggcacggtc gtcgtcacga gacccagcgg ttcggcgatc cgctgggtga gcaggtcggc
     2401 gtaggtcgtg ccggcggcgg ctgcgactgc atggccgagg agcatgaacc cgaggttgga
     2461 gtacgacggc cgctttcgac cgacgggtgt ggcgcgggcc gcaacgagca actcgtcgag
     2521 ggtttcgccg tacggattgg tgccgtgtcg ccagaggtcg agcgtggtgc gcacggatgc
     2581 cgtgcccttg gccaggcgcg gcagtccgga gcggtgggtg gcgagctcgc ccagcgtgac
     2641 ctgcgcgacc ggcgtccccg ccacgggcag gagggcaccg agcggcgtgt cgtcgctggt
     2701 ctcgccccgg cgtcgcgagt cgtcgaagag cagcccggtc accgccttgg agaccgatcc
     2761 gatctcgacc tgcgctcccg tctcgaggcc gacctgggcg atgtgcacct gtccccgcag
     2821 caccctggcg acgacgaagc ggtcatggtg ccgcccgagg tggtgggcgg cccgggccgc
     2881 gagggcggca tccccggagg tgcggatctc ggacatgtcc gcccaacggt tctcccgcgc
     2941 tgtcggtgcc ccgtgccaga gtcgcaccat gaacacatgg gtgtggggtc tgctggggct
     3001 ggccgtcggc gtcgctgcga cgatcgtcgt gactcgcttg gtggatgccg cgcgctcggc
     3061 cgccgtcgtc gccgagcgtg acctgctgag ggaacgggtg ctcgacctcg agacctcgct
     3121 cgccgaggac ctcgagaccg ccgccctgct cgcgccgctc aaggacgccc tggtccgtgt
     3181 cgagcaccag gtcggcacgc tcgaacgcga ccggatgcag cagttcggcg ccatccgggg
     3241 cctgctcgcc cgcgtcgagg gcgagaccca acacctgggt cgccagaccg cctcgctggc
     3301 gggctcactg cggtcctcga cggtgcgcgg tgcgtggggt gaggtgcagc ttcgccgggt
     3361 gctcgaggcc tcggggatgc tggcgcgctg cgacttcgac gagcaggtca ccgcggtcag
     3421 tgcccacgac cggggggtgc gcccggacgt cgtcgtgcgc ctgcccggcg acaaggtgct
     3481 cgtggtcgac gccaaggccc cgatggccgc cttcctggac gcccagggtg aagatctcac
     3541 cgatgacgag cgcggcgacc tgctgcgcga gcacgcggcc tcgctgggca gacacgtgtc
     3601 ggcgctggcc accaaggagt actggtcagc gtttcgcgag ggccccgaga tggtggtctg
     3661 cttcgtcccg agtgacgcca tgctcggtgc tgccctcgcg gcccagccgt cgctgcacga
     3721 ccaggcgctc tcgcagcggg tggtgctcgt cggcccgggc tcgctgatgg ccctgctgcg
     3781 caccgtggcg ttcacctggc aacaggacgc cctgtcctcc agtgcccgcg aactgctcga
     3841 cctcgggcgc gacctgcacc gacggctcgc gacgctcggc acccacaccg ccaaggtggg
     3901 gcgcagcctg cagtccagcg tcgaggccta caacgcgatg gtcggagccc tggagtcccg
     3961 cgtcatggtc accgcccgtc ggatgaacga cctcgacctc gtggacgacg agctgccggt
     4021 cctcacaccc gtggagagcg ccccccggcc gctgacggcg caggagctca tcgacgacgt
     4081 caccgcgcag gactcgcgcc ccgaactgct gctcgacctc gacgtcggtg agccggcacg
     4141 gccgcgggcg cacggcatcc gcgaggtggg ctgagccgcc agccgggacg gtcggttcgg
     4201 gagcgctggg ctggcccggt caggcaccca cgtactgcgc caggtgctgc ccggtcaggg
     4261 tcgagcgggc ggcgacgagg tctgacgggg ccccctcgaa gacgatcgtg ccgccgtcgt
     4321 gaccggcccc ggggccgagg tcgatgatcc agtcggcgtg cgccatcacg gcctggtggt
     4381 gctcgatgac gatgaccgac ttgcccgagt cgacgaggcg gtcgagcaac ccgaggaggt
     4441 tgccgacgtc ggcgaggtgc aacccggtcg tgggctcatc gagcacgtag atctcgccct
     4501 tgtcggccat ctgcacggcg agcttgaggc gctgacgctc accgccggac aacgtgctga
     4561 gcggctgacc gaggctgatg taacccagac cgacatcggc cagtcgcgcc aggatcgtgt
     4621 gcgccgccgg agtcctggct tcacccgagg cgaagaacac ctcggcctca tcgacagaca
     4681 gggcgagcac ctcggcgatg tccttgcccc ccagggtgta ctcgagcacc gacgcctgga
     4741 agcgcttgcc ctcgcagtcc tcacagggtg actccatcgt cgccatgatc ccgaggtcgg
     4801 tgatgatgac gcccgcgccg ttgcacgtgg ggcaggctcc ctcggagttc gagctgaaca
     4861 gggccggctt gaccccgttc accttggcga acgccttgcg gatcggctcg agcaggccgg
     4921 tgtacgtggc cgggttgctg cgccgcgacc ccttgatcgc gccctggtcc acgacgacga
     4981 catcgtcgcg cttggcgacc gagccgtgga tcagcgagct cttgccggag ccggcgacac
     5041 cggtcacgac gcacagcacc ccgaggggga tgtcgacgtc cacgccccgc aggttgtggg
     5101 tcgaggcgcc gcggacctcg accgcgccag tggccgcgcg cacgctcggc ttcagcgtgg
     5161 cccggtcgtc caggtgccgc cccgtgatcg tgtcgctggc gcgcagcccg tcgaccgacc
     5221 cctcgaagca gatcgtgccg ccgcccgagc cggcccccgg gccgaggtcg acgacgtggt
     5281 cggcgatggc gatggtctcg ggcttgtgct cgacgacgag cacggtgttc cccttgtcgc
     5341 gcaactgcag gagcagggtg ttcatccgct cgatgtcgtg cgggtgcagg ccgacggtgg
     5401 gctcgtcgaa gacgtaggtg acgtcggtga gcgaggaccc caggtggcgg atcatcttcg
     5461 tgcgctgggc ctcaccaccg gacagcgtcc cggccggccg gtcgagggag aggtagccca
     5521 ggccgatctc ggtgaacgag tcgagcaggt gctgcaaccc ggtgagcaag ggcgccaccg
     5581 acggctcgtc caggtcacgg acccactgcg cgaggtcgct gatctgcatc gcacacaggt
     5641 cggcgatgct cttgccgttg atcagggagg cgcgcgcttc ggcggtgagg cgggtgccgc
     5701 tgcactccgg gcaggcctgg aaggtgacgg cccgctcgac gaagcgccgc acgtggggct
     5761 gcatcgcttc cgggtccttg gacagcatcg acttctggat cttcgggatg atcccctcga
     5821 aggtgaggtt gaccccctcg accttgatct tcgtcggcgg cgcgaacagc atcgtgtcga
     5881 tctgcttggc ggtgaagtcg gcgatgggcg tgtccatcgg cagccccatg ccctcgaaca
     5941 gccggccgta ccagccgtcc atggagtagc cgggcaccgt cagcgcnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnngctc ggcatgaacc cctggaggaa ggcgctgtag
     6121 gtctcgttga tcatccgctt ggactccgcg gcgatcgtcg cgaagacgag cgagctcttc
     6181 cccgacccgg acaccccggt gaagaccgtg agcctgcgct ttggcagctc gacgttgacg
     6241 cccttgaggt tgttctcgcg agcacccagc acgcggatca ggtcgtgtga gtcagcgggg
     6301 tgtggcgtgg tcatgccggt caggctagac gccgacggcg acacccgtca gtgcaaaccg
     6361 gaggtgctgt cggcgaagct cgcgtcgtgg cgcatcgtgt cggccttgcc ggcggccttg
     6421 aggtcacgac ggatctcggc gggcagcgag aacatgatcg actcctccgc cgcgacgaca
     6481 ggtgacacat cgccgtagcc gcgagcctcg aggtactcga gcacctcgcg cacgaggatc
     6541 tcgggcaccg aggcacccga ggtcacaccc accgtcgaga ccccgtcgag ccaggactcg
     6601 tcgatctcgt ccgcgtagtc gaccaggtgc cccgcgggtg ttccgtgctc gagggcgacc
     6661 tcgaccagac gcaccgagtt ggaggagttg cgtgagccga tgacgagcat gaggtcgcag
     6721 tcggcggcca tctgcttcac ggcgagctgg cggttctgcg tggcgtagca gatgtcgtcg
     6781 ctcggggggt cctgcaggct ggggaagcgc tcacgaaggc gccgcaccgt ctccatggtc
     6841 tcgtcgaccg agagcgtggt ctgcgagagc cacacgacct tgtcggggtc gcggaccgtg
     6901 acgttgtcga cgtcgtcggg gccgtcgacg agctggatgt gctcgggggc ctcaccggcg
     6961 gttccgatga cctcctcgtg gccctcgtgg ccgatgagga ggatgtcgta gtcctcgtcg
     7021 gcgaagcgca cggcctcgcg gtgcaccttg gtgaccaggg ggcaggtggc gtcgatggtg
     7081 cgcagcgaca gggcacgagc ttcctcgtgc accaccgggg cgaccccgtg cgcggagaag
     7141 acgacggtcg caccctcggg cacctcgtcg gtctcgtcga cgaagatcgc accgcgcttc
     7201 tgcagcgtcg tgacgacgtg cttgttgtgc acgatctcct tgcggacgta gaccggggag
     7261 ccgtagaggt cgagagcctt ctcgacggtc accacggccc ggtccacccc cgcgcagtag
     7321 ccacgggggg cggcgaggag cacacgtttg ccggagtcag ccatgtcacc catggtaggt
     7381 cgccctcacg acgaccccga atccttgtgt ccggcaggtt ggcagtcagg gcagccaccc
     7441 tgccggacgc acggccgaac aatggccgtc ggagcccggc tctagggtga ctccatgacc
     7501 accctgcccg agcgcgccgc cgacaccacg gccgagcagc cctggccggt gcgcctgctc
     7561 tcgatgaaga tcggtgagta cgtcgagaag atgtcgaccc tgtgggtcga gggccaggtc
     7621 gtccagctca accgccgacc cggcgcccgc accgccttcc tcacgctgcg cgaccccgac
     7681 gtcgacatga gcctgtcggt ctccatccac gtcaacgccc tcgacgcgat gccgacccca
     7741 ctcggcgagg gcgcccgcgt cgtcctgcag gccaagccca cgttctgggc ccagcgcgga
     7801 tcgctcatgc tcgacgcccg ccagatccgc cccgtcggcg tcggcgagct gctcgcgcgc
     7861 ctggagtacc tcaagcgaca cctcgcgcag gaggggctgt tcgaccgcga gcgcaagaag
     7921 cccctgccgt tccttccccg acgcatcggg ctggtctgtg gccgggcgag cgcggccgag
     7981 cgcgacgtcg tcgagaacgc ccgccggcgc tggccctccg cggtgttcga gatccgtcag
     8041 gtcgcggtcc agggcacgga tgccgtaccg caggtgtgcg cggcgctcgc cgagctcgac
     8101 gcccaccccg aggtcgatgt catcgtcatc gcccgtggcg gcggcggggt cgaggacctc
     8161 ctgccgttca gcaacgagac catggtccgg gccgttgccg cggcgctcac ccccgtggtc
     8221 agcgccatcg gtcacgacgt cgacacccca ctgctcgacc tcgtcgccga ccaccgcgcc
     8281 tccacgccca cggatgccgc caaggcgatc gtcccggatg ccgctgccga gcgttcggcg
     8341 gtcgccgcgg ctcgcgctcg gggtcaccgc gcgctcaccg cacgcctgca ccacgaacgg
     8401 cggcggctcg tcgagctgcg cagccggccg gtgctcaccg acaagggcgc gttcgtgcgc
     8461 agccagcgct ccgtggtcga tgccctgcgc gaccgcagcc gccgccgggt cgaggcccag
     8521 ctgcaccggg cccgcgacca cgccgcgcac ctgcgcaccc aggtgcgcac cctgtcgccg
     8581 cagtcaacgc tcgaccgtgg ctacgcgatc gtgcagcacg ccgacggcgc ggtggtgagc
     8641 gacccggcat ccgtggtcgt gggtgaactg ctgcgcgtgc gcgtggcgcg cggcgacttc
     8701 ggcgtgaagc ctgtcgggca cgacggttag ggtttcggac atggcgacga cagcggattc
     8761 ggcaaccccc gagcaggggc ctgcccccga cggcgcgcag cacgccgaca tcgccggcct
     8821 gacctacgag caggcccgtg acgagctcgt cgacatcgtc gcccgcctcg agaacggcca
     8881 gtccgggctc gaggagagca tggggctctg gcagcggggc gaggccctgg ccgcccactg
     8941 cgcgacgtgg ttggaccgcg ccgaggcgaa gatcaccgtc caggagtgac cggagggtcg
     9001 gtgctcaggg agtgaccggc tggagggtct cgacgaactg cttcatctca tcgaaggtgg
     9061 ccgtgccggt gatgagcgtg gtcagcacac cctccccctc cggggcgtcg acgaggctgt
     9121 tctggacctt ggtgtcacgc tcgtacatcg tccaggtgcg ccccgcagcc tccagggtgc
     9181 cgaccttggg cgcacggttg gtctgcgacg cgatccattc ccggtttgga tcaagggtct
     9241 gctcgaccgc cacgtacgtg cccgaggggg tctggtaccc ggcgtgccac gtcgagaagc
     9301 cacccgtggt gacggtgtag cgggccgagg tcgcagtcca cccctccggc aggcccacgg
     9361 gctcgacgat cggccagccg gtgcgctcct gcacgtcgac ggccgtggcg tgcacgtcga
     9421 ccggcggccc actgaccgag ctgacccgcg ggacgatgag cacgaggacg gcgaccatcc
     9481 cgagcaccgc ggcgagggag tacaccaggt tcttggcaga ccccatcgag tagcggctgc
     9541 gggcgggcgc gggcgtggtc acccccctat cgtcgccgac gggtgcccgg gacgccgaat
     9601 caccctcgac gctagggttg gccctgccgt cacacgctca cgtcaggacg tcatggacac
     9661 cacgaacgac atgaacccga gcacagccga tcgcgccccc ggccgagcag tcgccgtcat
     9721 cggcgaactg cttgtcgaca tcgtgcacac ccccgacggc aacacggccg aacacgtggg
     9781 tggatcgccg gccaacgtcg ccctgggcct gtcccggctc gggcacgaga cgtggttcgc
     9841 gacgctcgtc ggcaccgacg atcgcgggat ccgctgcgcc gagcacgtcg agtccggcgg
     9901 agtgcacctc ctccccggca gcatcagcgc cagccacccc acctccaccg ccgccgcgac
     9961 gatcgacgag tcgggcgcgg cgagctacgt cttcgacctg cactgggacc tcgcccccgt
    10021 cgacctaccg gcggatgccg gtcacctgca catcgggtcc atcggcacca ccctgcgccc
    10081 cggcgacgag gaggtgaccg ccgcggtcca cgcgatccgc gcgaccggca cggtgtccta
    10141 cgaccccaac gtgcgcccga gcatcatggg cagccccgac gaggtccgcc cacgcatcga
    10201 ggagctcgtc gcactcagtg acgtcgtgaa gtgctccgag gacgacatcg cctggctcta
    10261 ccccgggcag tccccggccg aggtgatggc tcactgggcc gggctgggct cgacgctgac
    10321 gctcgtgacc ctcggcggcg acggggtcac gtggcgggcc ggcaccggcg acgtcgacca
    10381 cctgcccgcg ctcgccgacg acgtcgtcga caccgtcgga gcaggcgact ccttcatggc
    10441 cggcctcgtg tcgggcctgc tcgacgccgg gctgctcggc gacaccgcag cccgtgagcg
    10501 cctgcagcac gcgggcctgc ccgacgtgcg cccggccatc gaccgcgcgc tcgcgacgag
    10561 cggcatcacc gtgacccatg cgggcgccta cgccccgacc agggaggaac tggcgtgacc
    10621 gacttcgcct acgaggacct actgcccatc ggggacgacg agacccccta ccggctgctc
    10681 accaccgacg gcgtgtcggt cgtcgacggg ccgggcgggc gctcgttcct ccaggtcgag
    10741 cccgaggccc tgcgcctgct caccgacacc gccatgcacg acatcgcgca ctacctgcgc
    10801 ccggcgcacc tccagcagct ggcgaacatc ctcgatgacc cggaggcgag caacaacgac
    10861 aagttcgtcg cgctcgacct gctcaagaac gcgaacatcg ccgcgggcgg catcctgccc
    10921 atgtgccagg acaccgggac agcgatcgtc atgggcaagc gcggtcagca cgtgctgacg
    10981 cccgggcgcg acgaggaagc cgtgagccgt ggggtctacg acgcctacac ccggctgaac
    11041 ctgcgctact cccagatggc cccggtcacg acgtgggacg agcgcaacac cggctccaac
    11101 ctgccggccc agatcgagct ctacgccgac accgcccccg ggcacgagag cacctacaag
    11161 ttcctcttca tggccaaggg tggtggcagc gccaacaagt ccttcctgtt ccaggagacc
    11221 aaggccgtgc tcaaccccga cgcgatgatg cgcttcctcg acgagaagct gcgcagcctc
    11281 ggcaccgcgg cctgcccccc gtaccacctg gccatcgtca tcggcggcac cagcgccgag
    11341 ttcgccctca agacagccaa gtacgcgagc gccaagtacc tcgaccagct cccgaaggcc
    11401 ggggatgccg ccaccggcca cggcttccgc gacaccgagc tcgaggagaa ggtgctcgag
    11461 ctgacccggc agttcggcat cggcgcccag ttcggcggca agtacttctg ccacgacgtg
    11521 cgggtggtgc gcctgccccg ccacggggcc tccctcccca tcgccatcgc cgtgtcctgc
    11581 tcggccgacc gccaggtgct cggcaagatc acccccgagg gcgtgttcat cgaacagctc
    11641 gagaccgacc cggcccgctt cctgcccgac cagacccacg ccgccctcgc cggtgaggac
    11701 gacgtggatg ccgtcggcgc ggcatccacg ggtgccggcg ccgtcgtgtc catcgacctc
    11761 aaccagccga tggccgacat cctcgccgag ctcaccaagc acccggtcaa gacgcggctg
    11821 tccctgaccg gcccgctcgt cgtggcccgc gacatcgccc acgcgaagat caaggaacgc
    11881 ctcgacgccg gtgagccgat gcccgactac ctgcgcaacc acccggtcta ctacgcgggc
    11941 ccggcgaaga cccccgaggg gatgccgtcg ggatcgttcg ggccgacgac cgcaggccgg
    12001 atggactcct acgtcgacca gttccaggcc gctggcggct cgatggtcat gctggcgaag
    12061 ggcaaccgca gccagcaggt gaccgatgcc tgcaaggctc acggcggctt ctacctcggg
    12121 tcgatcggcg gcccggccgc gcgcctggcc caggactgca tccgcagcgt cgaggtgctc
    12181 gagtaccccg agctgggcat ggaggccatc tggaagatcg aggtcgagga cttcccggcc
    12241 ttcatcgtcg tcgacgacaa gggacatgat ttcttcgcct cggtgaccaa gccggtggcg
    12301 ttcaccatcg agaagcgaac ggggttgctc tgatgccatc cacctttgat gacctgctcg
    12361 aggccaaccg ggcctacgcc gcggagaacc cgatcctcgg cttcgacggt gtggcccacg
    12421 ccggcgtcgc cgtcgtcacc tgcatggaca gccggatcga cccgctgcgg atgatcgggc
    12481 tcaagcc
//
LOCUS       DAOSOC010000061        12485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5048_length_12485_cov_8.430973, whole genome shotgun sequence.
ACCESSION   DAOSOC010000061 DAOSOC010000000
VERSION     DAOSOC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12485
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5048_length_12485_cov_8.430973"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..875
                     /gene="dapB"
                     /locus_tag="PL014_06380"
     CDS             129..875
                     /gene="dapB"
                     /locus_tag="PL014_06380"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOT56753.1"
                     /translation="MGATRVAVIGASGRMGATVCAAVEAASDLELVGRFDVGDDLGNL
                     AGAQVVVEFSVPDASPANVAHCVAQGVHVVVGTTGWSDERLATLREQVAQAPGVGVLI
                     APNFALGAVLMMAFAAKAASYYESVEVIELHHPDKVDAPSGTAARTASLIGEAREAAG
                     LGPVPDATTHDPDGARGARVNGIPVHSVRLRGLVAHQEVLLGAAGEMLTIRHDSFDRV
                     SFMPGVLAAVRAVADHPGVTVGLEHYLGLG"
     gene            1118..2527
                     /locus_tag="PL014_06385"
     CDS             1118..2527
                     /locus_tag="PL014_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT56754.1"
                     /translation="MTTTITPGATSTAPQAELGVVGPGRWIERWDANDDHFWAGRGRA
                     IARRNLGFSIFAEHIGFSVWMLWSIVVVSMSGTFDASGALVTSGANGWALTAGQALTV
                     LGVASGVGALLRIPYTFAVPVFGGRNWTVVSALLLLVPTLGLAWVVQHPEVPFWVLLL
                     VAGTAGFGGGNFASSMANITFFYPEKEKGWALGLNAAGGNIGVAVAQKLVPLAIGLGG
                     VAAGLSNAGFLYVPFAIASAVLAYYFMDNLREAKADPGPTARATGHLHTWLMALLYIG
                     TFGSFIGYSAAFPTLLKVVFERPDIALTYGFLGALVGSLSRPLGGRLSDRLGGSVVTI
                     VSFVAMAVAGVIAVVGVQQKSLPIFFASFMALFVATGVGNGSTYRMIPAIFTRLAARD
                     GGEDSALEYRRRAAGAVGIIAAVGAFGGFVIPFVYKFAREEYGSIVPALQAYVLVFLA
                     LAVLTWAVYVRRGATMAKV"
     gene            2535..4664
                     /locus_tag="PL014_06390"
     CDS             2535..4664
                     /locus_tag="PL014_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT56755.1"
                     /translation="MPTPTHCPYCALQCAQALHATPDAVSPVRVEARDFPTNRGGMCQ
                     KGWSSAEVLTATDRLTTPLVRRVGADGGRGALGPATWEEALDLVHSRLAGIQAVHGRD
                     AVAVFGGGGLTNEKAYTLGKFARVVLGTPYIDYNGRFCMSSAAAGANRSLGVDRGLAF
                     PLADLGGADAVLVVGSNPAATMPPLVQHLAGARSRGGLVVVDPRASATARLTDGGAGI
                     HLAAVPGSDLVVLLALTHVLVDEGLVDEEYVTTRTTGWDAVRASVARWWPERAEAVCG
                     IPADTLRATARLLAAAAPAHGGRGAYVLTGRGLEQSTQGTDGVSAAINLAVALGLPGR
                     PGSGYGPLTGQGNGQGGREHGLKSDQLPGYRMITDPAARSHVAGVWGVDPATLPGPGV
                     PAVELLSRLGTAGGPKALLVHGANPAVSAPNVSAVRAALGRLDLLVVSDVVPSETAQL
                     ADVVLPVAQWAEEEGTMTTLEGRVVRRRRAIDPPGQARSELWVWARLAERFGHHLSDD
                     ACEVFDELARASAGGRADYSAITYARLDAGEALFWPCSAAAPQGTPRMFLDRFPTDDG
                     RAHLFAVDHHGPDDDVAASMPVHLVTGRVLHHYQSGAQTRRVAELVEAAPVPLLEIHP
                     LLADTHGVTEGDAVRVTSARGELVATASVTEAIRPDTVFLPFHWAGDLSANLLTNDAV
                     DPVSGMPEFKVCAVRLTRVRHLEEVLA"
     gene            4661..6166
                     /locus_tag="PL014_06395"
     CDS             4661..6166
                     /locus_tag="PL014_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT56756.1"
                     /translation="MRRVVVVGHGMVGSRFVEELVGADPDVVVTVLGKEPLPAYNRVL
                     LSSVVAGAKSPQALGLAGPAHPRVEVLTGVAAAGIDRERRVVVDDRGTAHGYDELVLA
                     TGSAARVPSIQGVAYDEGLVDGVFVLKDMADATGIVAAAGSGRRAVVLGAGVLGIEVA
                     TGLARRGLAVRVVHLADRLMERQLGWEASQVAVASLERLGITTHVGASVVGVRTRRGR
                     LESVRFADGVEAAADLFVMCTGTVPETMLARRAGLTVDRGVVVGDDLTSPDDPHVHAI
                     GDCAQPPGGASGLVAQGWQQASRLVARLTARSVTVTEGGTHDVVRVKASGMSMVSMGV
                     CGDVDRDDPRYRILSLKDPEAGRYVEVVVSAGVLVGATCIGDADVATTLSALYTRSLP
                     VPEDPALLLVRALAGGGTASAAKRPDELADADRVCTCNSVTAGRIRDAVRGGCTSVAD
                     VAAATRASTGCGDCAGVVEALISSQTPTDATQPPTHSHTETGARALVRSQP"
     gene            6163..8748
                     /gene="nirB"
                     /locus_tag="PL014_06400"
     CDS             6163..8748
                     /gene="nirB"
                     /locus_tag="PL014_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HOT56757.1"
                     /translation="MSEHVVVVGGGMVAHRFVEALTARDTGSRFHVTVLAEEGRAPYD
                     RVGLSAFFSGSTPDDLTLGDPGLWESPRATLRTGSRAVSLDREARTVTTAEGATVGYD
                     HLVLATGSYAWVPPVPGADLAGVFVYRTIDDVVALRDWVGRREAELGRPVRGAVIGGG
                     LLGLEAAGALVGLGAGCTVVEFAPRLMALQVDEGGGHALRRTIEAMGVDVRVGTASES
                     IDADADGRVAGLRLAGADEAVPVDVVVFATGVRPRDELARPAGLPTGERGGVVVDEGC
                     RTVDPRVWAVGEVACIEGRCLGLVAPGYTMAEIAVDRLLGGDATFPGADLSTKLKLLG
                     VDVASFGDAFASTPGSLELVHSDPVAGVYKKLVVTDDARTLLGGVLVGDASAYASLRP
                     MVGAELGGDPGLWLLPENAGAGRPQVDLPAEAAVCSCNNVTAGAVRGAVTDHQCHDVA
                     AVKACTRAGTSCGSCIPLVKKIMGAEMARAGVALSTALCEHVAMSRAELFSAVTVSGL
                     RTFSEIITRFGSGRGCDICKPVIASILASLYDEHVLGGDRAALQDTNDRYLANLQKDG
                     TYSVVPRVPGGEILPEQLVLIGRIAQDYGLYTKITGGQRIDLFGARLEQLPGIWQRLV
                     DGGMESGHAYGKSLRTVKSCVGSSWCRFGVQDSVGMAVLLELRYRGLRSPHKIKLGVS
                     GCARECAEARGKDVGLIATDNGWNIYVGGNGGFTPRHAELLVEDVDDETAIAIIDRYL
                     VYYVRTADRLQRTAPWQQEHEGGLDRIREIVVDDALGIAADLEAHMARHVDDYEDEWA
                     AVLRDPERLRQFTSFVNAPDVADPTLAYVTERGQRRPATAAERAGGTASGPVLIAPTT
                     LEVRS"
     gene            8745..9122
                     /gene="nirD"
                     /locus_tag="PL014_06405"
     CDS             8745..9122
                     /gene="nirD"
                     /locus_tag="PL014_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HOT56758.1"
                     /translation="MTAIADRLTRVRICSYDELTPELGVAALVLGEQVAVFRLSDGRV
                     FAVSNTCPFSGAAVMSRGITGSRGEEPTITSPVYKQVFSLVDGRCLDTVDKAPVPGHG
                     PDLAVHTVSVDDGQVTIDLTGPA"
     gene            9119..10351
                     /gene="cobA"
                     /locus_tag="PL014_06410"
     CDS             9119..10351
                     /gene="cobA"
                     /locus_tag="PL014_06410"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOT56759.1"
                     /translation="MSTLVGLDLTGRRVVVVGAGAVGTRRARRLIEDGARVVLVDPSP
                     SDDARRMASHGDVEVVERGVAASDVDGAWLVVTATADPALNAEVAQWCLEGRTWCVNA
                     SDGRGGTARVAASSTHGDLTVGVVSVGDPDPRRVSTVHDALAAHVESGAVDLRRRRKR
                     RGRVVLVGSGPGDPGLVPVAGLEALATADVVVTDRLGATALLDRLPADVEIVNVGKSP
                     TNHPVPQSEINAILVDRALQGLTVVRFKGGDPYVFGRGGEEVHACRQAGVEVTVVPGI
                     TSAFSVPALAGIPVTQRGVATSVHVASGHVGADAAALAALAAGSTVVFLMGVAALPGI
                     CVAALEQGVAPDLPVAMIEQGSTATERVTRATVGTAGDVVRQAGVRAPAIIVMGRVAA
                     QGFLDDPVCATTGVADDD"
     gene            10344..11480
                     /locus_tag="PL014_06415"
     CDS             10344..11480
                     /locus_tag="PL014_06415"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOT56760.1"
                     /translation="MTETARRPGDAVAGPALAGCVIAVTADRRFKELANALERRGATV
                     EHAPALVVVPHLDDESLVEHTRVIIDAPPDVVVATTGIGFRGWIEAADAAGLAEDLLD
                     VFASARLIARGPKARGAIEAAGLTADWVAQSETAAEIEEFLLAEGVDGVRIAVQHHGS
                     GADGLDEAFAAAGALVSSVVVYSWGASDPDVLAASVGRTAQGLHDAVVFTSAPGVHAW
                     LDTASEEQLDAIRERNASGQLVLATVGPITAAPLAAVGLVPLVPGRWRLGAVVRELVT
                     HYAQQPTLETVAGPLAVRARGAVLDGRALTLTPAGADILAALARAEGAVVPRERLQAL
                     LPGGSSDGHAVEAAIARLRECCGSKDVVRTVVKRGYALALVHPS"
     gene            11477..12154
                     /locus_tag="PL014_06420"
     CDS             11477..12154
                     /locus_tag="PL014_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOT56761.1"
                     /translation="MSAPLIVCAHGTRDPAGRGTIAAVVAAVAARLPDVAVLEAYVDV
                     HGPDVVEVVDGLPAGEGGVVVPLLLAGGYHVHVDIAQAVARRPDVVATPALGPDDRLV
                     DIVLDRLRSSGATAGMPVVLVPAGSSDARAQADSAAVAEALARRWGGPVTLGFAAGPQ
                     PSAADAVVLARESSGGRVALASYLLAPGFFQRRLEDAGAAVVTGPLAPDERIVDIVVD
                     RYRVATS"
     gene            complement(12186..>12485)
                     /locus_tag="PL014_06425"
     CDS             complement(12186..>12485)
                     /locus_tag="PL014_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOT56762.1"
                     /translation="AVLIASLLSVAVKLAGYLVPESVMAGERTRRVTTLLPVALLAAL
                     VVVQSVTGDAGAIVVDARLAAAGLAIVLLLLRVNFLVIVLAAGALAAILRALGWG"
BASE COUNT         1510 a         4641 c         4572 g         1762 t
ORIGIN      
        1 gtccctcctc tcggggtggt atgtcacgtc ctgagccaag ggtaaccgaa gatggactga
       61 agatgaacgt aaggtgaacg acccggcgtg gcgagggcgt ggccggtgcc gccactacag
      121 tgctcgccat gggtgcgaca cgggtggccg tcatcggtgc gagtgggcgg atgggcgcga
      181 ccgtgtgcgc cgccgtcgag gcggcgagcg acctcgagct cgtcggtcgg ttcgacgtgg
      241 gcgacgacct cggcaacctc gccggtgcgc aggtcgtcgt cgagttctcg gtccccgacg
      301 cctccccggc caatgtcgcc cactgcgtgg cccagggggt gcacgtcgtc gtgggcacga
      361 ccggctggtc cgacgagcgg ctcgcgaccc tgcgcgagca ggtggcgcag gcacccgggg
      421 tcggcgtgct catcgccccc aacttcgccc tcggagcggt gctcatgatg gccttcgccg
      481 cgaaggcagc cagctactac gagtccgtcg aggtcatcga gctgcaccac ccggacaagg
      541 tcgacgcccc ctcgggtacc gcagcgcgca cggcatccct catcggtgag gcccgcgagg
      601 ccgccgggct cgggcccgtc cccgacgcga ccacgcacga cccggacggc gcgcgcgggg
      661 cccgcgtgaa cggcatcccg gtgcactccg tgcgcctgcg cggtctcgtg gcgcaccagg
      721 aggtgctgct cggtgcggcg ggggagatgc tgacgatccg ccacgactcc ttcgaccggg
      781 tgtcgttcat gccgggtgtc ctggcggcgg tgcgcgccgt cgcagaccac cccggggtca
      841 cggtgggcct cgagcactac ctcggcctcg gttgaccttc cgggggtgcg ccggcacccc
      901 aaccgccccc cgaggccccg ccccacctgc acgtatgacg gtcgtgtttc cgacttctca
      961 cggtgcctcc ccgcgctcgg gtgcggacga tgttgcggcg cattcacacg ccccgaaggc
     1021 cctcgaaaca ccgcttccct agcgtcggtg ctgtccgaca acgaggtggg cggcctcgac
     1081 gtcgtccccc cgcacacgta cttcgggaag ggcgaccatg accaccacca tcaccccggg
     1141 cgcgaccagc accgcgccgc aggccgagct cggcgtcgtc gggcccggtc gctggatcga
     1201 gaggtgggac gccaacgacg accacttctg ggccggccgc ggacgggcca tcgcccgccg
     1261 caacctcggt ttctcgatct tcgccgagca catcggcttc tccgtctgga tgctgtggtc
     1321 catcgtcgtc gtctcgatgt cgggcacctt cgacgcgtcc ggcgcgctcg tgacgagcgg
     1381 ggccaacggc tgggcactca cggccggcca ggcgctcacc gtcctcggtg tcgcctccgg
     1441 cgtcggcgcc ctcctgcgca tcccgtacac gttcgcggtg cccgtcttcg gcggccggaa
     1501 ctggaccgtc gtctccgcac tgctcctcct cgtgccgacg ctcggcctgg cctgggtcgt
     1561 ccagcacccg gaggtcccct tctgggtgct cctgctcgtc gcgggcaccg cgggcttcgg
     1621 cggcggcaac ttcgcctcct cgatggccaa catcaccttc ttctaccccg agaaggagaa
     1681 gggctgggcc ctgggcctga atgccgcagg cggcaacatc ggggtcgcgg tcgcccagaa
     1741 gctcgtgccg ctcgcgatcg gcctcggcgg cgtcgcggcg gggctgtcga acgccggctt
     1801 cctgtacgtc cccttcgcga tcgcctcggc cgtgctcgcc tactacttca tggacaacct
     1861 gcgcgaggcc aaggccgacc ccggccccac ggcccgcgcc acgggacacc tgcacacctg
     1921 gctcatggcg ctgctctaca tcggcacctt cggctcgttc atcggctact cggcggcatt
     1981 cccgaccctg ctcaaggtcg tcttcgagcg gcccgacatc gccctgacct acggcttcct
     2041 cggagcgctc gtcggctcgc tgtcccgccc gctcggtggt cggctctccg accggctcgg
     2101 cggctcggtc gtcacgatcg tcagcttcgt cgccatggcg gtggccggcg tcatcgccgt
     2161 cgtcggggtg cagcagaaga gcctgccgat cttcttcgcg agcttcatgg ccctgttcgt
     2221 cgcgaccggt gtcggcaacg gctcgacgta ccggatgatc ccggcgatct tcacccggct
     2281 cgcggcccgc gacggcggcg aggacagtgc gttggagtac cgccggcgcg cagcgggtgc
     2341 cgtcggcatc atcgcggcgg tcggcgcctt cggcgggttc gtcatcccct tcgtctacaa
     2401 gttcgcccgc gaggagtacg gctcgatcgt ccccgccctg caggcctatg tcctggtctt
     2461 cctcgccctc gcggtgctca cctgggccgt ctacgtgcgc cgcggcgcca cgatggccaa
     2521 ggtctgagga caccgtgccc acccccaccc actgccccta ctgcgccctc cagtgcgcgc
     2581 aggccctcca cgcgaccccg gatgccgtgt caccggttcg cgtcgaggcg cgcgacttcc
     2641 ccacgaaccg gggaggcatg tgccagaagg ggtggagctc agccgaggtg ctcaccgcca
     2701 ccgaccgcct caccacgccg ctcgtgcgac gggtcggggc ggacggtggg cggggggcgc
     2761 tcgggccggc cacgtgggag gaggctctcg acctcgtgca ctcgcggctc gccggcatcc
     2821 aggccgtgca cggccgggac gcggttgccg tgttcggcgg aggcggcctc accaacgaga
     2881 aggcctacac cctgggcaag ttcgcgcggg tcgtgctggg cacgccgtac atcgactaca
     2941 acggccggtt ctgcatgtcc tcggccgcgg ccggcgccaa ccgcagcctc ggcgtcgacc
     3001 ggggcctggc cttcccgctc gcggacctcg ggggcgcgga cgccgtgctc gtcgtcggct
     3061 ccaaccccgc agcgacgatg ccgcccctcg tgcagcacct cgccggcgcg cggtcgcgcg
     3121 ggggcctggt cgtcgtcgac ccgcgggcct cggcgacggc acggctgacc gacggcgggg
     3181 ccggcatcca cctcgccgcc gtgccgggca gcgacctcgt cgtcctgctc gccctcaccc
     3241 acgtcctcgt cgacgagggg ctcgtcgacg aggagtacgt gaccacccga accaccgggt
     3301 gggatgccgt gcgcgcctcg gtcgcgcggt ggtggcccga gcgcgcggag gcggtgtgcg
     3361 gcatccccgc cgacaccctg cgcgccactg cccgcctgct cgcggccgcg gcgcccgcgc
     3421 acggcggacg gggcgcctac gtgctcaccg gaaggggcct ggagcagtcg acccagggca
     3481 ccgacggcgt gtccgcggcg atcaacctcg ccgtcgcgct cggcctgccc ggccgccccg
     3541 gttccggcta cggaccgctc accggtcagg gcaacgggca gggcgggcgc gagcacggcc
     3601 tcaagagcga ccagctgccc ggctaccgga tgatcaccga ccccgctgcg cggtcccacg
     3661 tcgccggggt gtggggagtc gacccggcca cgctgccggg ccccggcgtc ccggccgtcg
     3721 agctgctctc ccggctcggc accgccggag ggccgaaggc gctgctcgtg cacggggcca
     3781 accccgccgt ctccgcgccc aacgtctcgg ccgtgcgcgc ggcgctcggc cgcctcgacc
     3841 tgctcgtcgt cagcgacgtc gttccttcgg agaccgccca gctcgccgac gtcgtgctcc
     3901 ccgtggcgca gtgggccgag gaggagggca cgatgaccac cctcgagggc cgggtcgtgc
     3961 gccgccgcag ggccatcgac ccgccggggc aggcccgctc cgagctgtgg gtgtgggccc
     4021 ggctggccga gcgcttcggt caccacctgt ccgacgacgc ctgcgaggtc ttcgacgagc
     4081 tggcccgcgc ctcggcgggc gggcgggccg actactccgc gatcacctac gcccggctcg
     4141 acgccgggga ggcgctgttc tggccgtgct cggctgccgc gccgcagggc acgccgcgga
     4201 tgttcctgga ccgcttcccg accgacgacg gccgcgccca cctcttcgcg gtcgaccacc
     4261 acgggcccga cgacgacgtc gcggcctcga tgccggtgca cctcgtcacc gggcgcgtgc
     4321 tgcaccacta ccagtcgggg gcgcagaccc gccgggtcgc cgagctcgtc gaggcggcgc
     4381 cggtgccgct gctcgagatc cacccgctgc tcgccgacac gcacggcgtc accgaggggg
     4441 atgccgtgcg cgtcacgtcg gcgcgcggcg agctggtcgc gaccgcgtcg gtgaccgagg
     4501 cgatccgccc cgacaccgtc ttcctgccgt tccactgggc cggtgacctc agcgccaacc
     4561 tgctgaccaa cgatgcggtc gacccggtgt ccggcatgcc ggagttcaag gtgtgcgccg
     4621 tgcggctgac gcgggtccgc cacctcgagg aggtcctcgc atgagacgcg tcgtcgtcgt
     4681 cggccacggg atggtcggct ccaggttcgt cgaggagctc gtcggcgccg acccggacgt
     4741 cgtggtgacg gtgctcggca aggagccact gcccgcctac aaccgggtgc tgctgtccag
     4801 tgtcgtcgcg ggggccaaga gcccacaggc cctggggctg gcgggcccgg cccacccgag
     4861 ggtcgaggtc ctcacgggcg tcgcggccgc cgggatcgac cgcgagcgcc gtgtcgtcgt
     4921 cgacgaccgc gggacggcgc acggctacga cgagctcgtg ctcgccaccg gatcggccgc
     4981 ccgggtgccg tccatccagg gcgtcgccta cgacgagggg ctggtggacg gcgtcttcgt
     5041 gctcaaggac atggccgacg ccaccgggat cgtcgcggcg gcagggagcg gccgtcgggc
     5101 cgtcgtcctc ggtgcgggag tgctcggtat cgaggtcgcg acgggactgg cccgccgtgg
     5161 gctggccgtg cgggtcgtcc acctcgccga ccggctgatg gagcgtcagc tcgggtggga
     5221 ggcctcgcag gtggccgtgg ccagcctgga gcgcctcggc atcaccaccc acgtgggcgc
     5281 ctcggtcgtg ggtgtccgca cccggcgcgg acgcctggag agcgtgcgat tcgccgatgg
     5341 ggtcgaggcg gccgccgacc tcttcgtcat gtgcaccggc actgtgccgg agaccatgct
     5401 cgcccgccgg gccgggctca ccgtcgaccg cggcgtcgtc gtcggggacg acctcaccag
     5461 cccggacgac ccgcacgtgc acgccatcgg tgactgtgcc cagccccccg ggggcgccag
     5521 cgggctcgtc gcccagggct ggcagcaggc gagccgcctc gtcgcccggc tcacggcccg
     5581 gtcggtgacc gtgaccgagg gcgggaccca cgacgtcgtg cgggtcaagg cctcggggat
     5641 gtcgatggtg agcatgggtg tctgtggcga cgtcgaccgt gacgacccgc gctaccggat
     5701 cctgtcgctg aaggacccgg aggccggccg ctacgtcgag gtcgtcgtgt ccgcaggggt
     5761 cctcgtcggt gcgacgtgca tcggcgacgc cgacgtcgcg acgaccctgt cggcgttgta
     5821 cacgcgatcc ctccccgtgc ccgaggaccc ggccctgctc ctggtgcggg cgctcgcggg
     5881 cggtggcacg gcatccgcgg cgaagcgacc cgacgagctc gcggacgccg accgggtgtg
     5941 cacgtgcaac agcgtcaccg ccgggcggat ccgggatgcc gtgcgcggcg gctgtacctc
     6001 cgtcgccgat gtcgccgcag cgacccgggc cagcaccggg tgtggtgact gcgccggagt
     6061 cgtggaagcc ctgatcagca gccagacccc caccgacgcc acccagccac ccacccacag
     6121 ccacaccgag accggggccc gggccctggt gaggagccag ccatgagcga gcacgtcgtc
     6181 gtcgtcggcg ggggcatggt cgcccaccgc ttcgtggagg cgctgaccgc ccgggacacc
     6241 gggtcgcgct tccacgtcac cgtgctcgcg gaggagggcc gggcgcccta cgaccgggtc
     6301 ggcctcagtg ccttcttctc gggctcgacg cccgacgacc tcacgctcgg cgacccgggc
     6361 ctgtgggagt ccccccgggc caccctgcgc accgggtcgc gggcggtgtc cctggaccgc
     6421 gaggcgcgga cggtgacgac cgccgagggc gccaccgtgg gttacgacca cctcgtgctc
     6481 gcgaccggct cgtacgcgtg ggttcctccg gtgccgggcg ccgacctcgc cggggtcttc
     6541 gtgtaccgga ccatcgacga cgtcgtcgcc ctgcgcgact gggtcggacg ccgggaggcc
     6601 gagctcggcc ggccggtgcg cggcgccgtc atcggtggcg gcctgctcgg cctcgaggcg
     6661 gcgggggcgc tggtggggct gggcgccggg tgcaccgtcg tcgagttcgc cccccgcctc
     6721 atggccctcc aggtggacga gggtggtggc cacgccctgc gccgcaccat cgaggcgatg
     6781 ggcgtcgacg tgcgggtcgg cacggcatcc gagtcgatcg acgcggatgc ggacgggcgg
     6841 gtcgcggggc tgcgcctcgc gggtgccgac gaggcggtgc cggtcgacgt cgtcgtgttc
     6901 gcgaccggcg tgcggccacg cgacgagctc gcccggcccg ccggcctccc gacgggtgag
     6961 cgcggcggcg tcgtcgtcga cgagggctgc cgcacggtcg acccgcgggt ctgggcggtc
     7021 ggagaggtcg cctgcatcga gggacgctgc ctgggcctgg tcgcaccggg ctacacgatg
     7081 gccgagatcg ctgtcgaccg gctgctcggc ggcgacgcca cgttccccgg cgccgacctg
     7141 tcgaccaagc tcaagctgct cggcgtcgac gtcgcatcct tcggtgacgc gttcgcgagc
     7201 accccgggct ctctcgagct ggtccactcc gacccggtgg ccggcgtgta caagaagctc
     7261 gtcgtcaccg acgacgcccg caccctcctc ggcggggtgc tcgtcgggga cgcgtcggcc
     7321 tatgcctcgc tgcggccgat ggtcggggcc gagctcggcg gcgaccctgg gctgtggctt
     7381 ctgcccgaga acgccggcgc ggggcggccc caggtcgacc tgcccgccga ggcggccgtc
     7441 tgctcgtgca acaacgtcac cgcgggtgcg gtgcgcgggg ccgtgaccga ccaccagtgc
     7501 cacgacgtcg ccgccgtcaa ggcgtgcacc cgggcaggga cctcgtgcgg gtcgtgcatc
     7561 cccctcgtga agaagatcat gggcgccgag atggcccggg ccggcgtcgc cctgtcgacc
     7621 gcgctgtgcg agcacgtcgc gatgtcgcgg gccgagctgt tcagcgccgt cacggtgagt
     7681 gggctgcgca ccttcagcga gatcatcacg cgcttcgggt ccggccgcgg ctgcgacatc
     7741 tgcaagccgg tcatcgcgtc gatcctcgcc tccctgtacg acgagcacgt gctcggtggc
     7801 gaccgtgcgg ccctgcagga cacgaacgac cggtacctgg cgaacctgca gaaggacggg
     7861 acgtactccg tcgtcccgcg ggtgccgggc ggtgagatcc tccccgagca gctcgtgctc
     7921 atcggccgga tcgcccagga ctacgggctc tacacgaaga tcaccggcgg ccagcggatc
     7981 gacctgttcg gggcccggct cgagcagctg cccggcatct ggcagcggct cgtcgacggc
     8041 gggatggagt ccggccatgc gtacggcaag tcgctgcgca ccgtgaagtc ctgcgtcggc
     8101 tcgtcgtggt gccgcttcgg ggtgcaggac tcggtcggga tggcagtgct cctcgagctg
     8161 cgctaccgcg ggctgcgcag tccgcacaag atcaagctcg gggtctccgg ctgtgcccgt
     8221 gagtgcgccg aggcgcgcgg caaggacgtc ggcctcatcg ccaccgacaa cggctggaac
     8281 atctacgtcg gcggcaacgg cgggttcacc ccacggcacg ccgagctgct cgtcgaggac
     8341 gtcgacgacg agaccgccat cgcgatcatc gaccggtacc tcgtctacta cgtgcgcacc
     8401 gccgaccgcc tccagcgcac cgcgccctgg cagcaggagc acgagggcgg gctggaccgg
     8461 atccgcgaga tcgtcgtcga cgacgccctc ggcatcgccg cggacctcga ggcgcacatg
     8521 gcccgccacg tcgacgacta cgaggacgaa tgggccgcgg tgctgcgcga ccccgagcgg
     8581 ctgcgccagt tcacgtcctt cgtcaacgct cccgacgtcg cggacccgac cctcgcctac
     8641 gtcaccgagc gcggccagcg acggccggcg acggccgccg agcgcgccgg gggcacggca
     8701 tccggccccg tcctcatcgc cccgaccacc ctggaggttc gctcatgact gccatcgccg
     8761 accggctgac aagggtccgc atctgttcct acgacgagct cacgcccgag ctgggcgtcg
     8821 cggccctcgt cctcggcgag caggtcgccg tcttccggct ctcggacggg cgggtgttcg
     8881 cggtgtccaa cacgtgcccg ttctcgggtg ccgcggtcat gtcccgcggc atcaccggct
     8941 cccgcgggga ggagccgacg atcacgtcgc cggtgtacaa gcaggtcttc tcactggtgg
     9001 acgggcgctg cctcgacacc gtggacaagg cacccgtgcc gggacatgga ccggacttgg
     9061 cggtacacac cgtgagcgtt gacgatggtc aggtgaccat cgacctcacg ggccctgcgt
     9121 gagcacgctc gtcggcctgg acctcaccgg acgtcgcgtc gtcgtcgtcg gcgccggtgc
     9181 ggtcggcacg cggcgggcac gcaggctgat cgaggacggc gcgcgcgtcg tgctcgtcga
     9241 cccgagcccc agcgacgacg cccggcggat ggcgagccac ggcgacgtcg aggtcgtgga
     9301 gcgcggtgtc gcggcatccg atgtcgacgg cgcctggctc gtcgtcacgg cgacggctga
     9361 tccggcgctg aacgccgagg tcgcccagtg gtgcctggag ggtcgcacgt ggtgcgtcaa
     9421 cgcctccgac ggccgaggtg gcacggcgcg ggtggccgcc tcgagcaccc acggcgacct
     9481 caccgtcggc gtggtgtccg tgggcgaccc cgacccgcgc cgcgtctcca ccgtgcatga
     9541 cgcgctggcc gcacacgtcg agtcgggggc ggtcgacctg cgccggcggc gcaagcgccg
     9601 ggggcgggtg gtgctcgtcg gcagcggtcc cggcgacccg ggcctggtgc cggtggccgg
     9661 cctcgaggcc ctcgccacgg ccgacgtcgt cgtcaccgac cggctcggcg cgacggccct
     9721 gctcgaccgg ttgcctgccg acgtcgagat cgtcaacgtc ggcaagagcc ccacgaacca
     9781 ccccgtgccg cagtccgaga tcaacgcgat cctcgtcgac cgcgcgctcc aggggctgac
     9841 cgtcgtgcgc ttcaagggcg gggacccgta cgtcttcggc cgcggcggcg aggaggtgca
     9901 cgcctgtcgg caggcgggcg tcgaggtcac cgtcgtgccc ggcatcacct cggccttcag
     9961 cgtgcccgca ctggccggga tcccggtcac gcagcgcggc gtcgcgacgt ccgtgcacgt
    10021 ggcgtcgggg cacgtcggag cggatgccgc cgccctcgcc gcgctcgcgg ccgggtcgac
    10081 cgtcgtgttc ctcatgggtg tcgccgccct gccgggcatc tgcgtcgcgg ccctcgagca
    10141 gggcgtggcg ccggacctcc cggtggcgat gatcgagcag gggtcgaccg ccaccgagcg
    10201 ggtgacccgg gcgaccgtcg ggacggccgg ggacgtggtt cggcaggccg gggtgcgggc
    10261 tccggcgatc atcgtcatgg ggcgggtcgc ggcgcagggt ttcctcgacg accccgtgtg
    10321 cgcgacgacc ggcgtggccg acgatgactg agacggcgcg ccggcccggc gacgcggtgg
    10381 ccggcccggc gctcgcgggc tgcgtcatcg ccgtcaccgc cgaccgccgg ttcaaggagc
    10441 tcgccaacgc cctcgaacgc cgcggggcca cggtcgagca cgctccggcg ctcgtcgtcg
    10501 tgccccacct cgacgacgag agcctcgtcg agcacacgag ggtgatcatc gacgccccac
    10561 ccgacgtggt cgtcgcgacg accgggatcg gcttccgtgg ctggatcgag gcggcggatg
    10621 ccgctgggct ggccgaggac ctgctcgacg tcttcgccag tgcccgcctc atcgcccggg
    10681 gccccaaggc acgcggagcc atcgaggccg ccgggctcac cgccgactgg gtggcccagt
    10741 ccgagacggc tgccgagatc gaggagttcc tgctcgccga gggagtggac ggggtgcgga
    10801 tcgccgtcca gcaccacggc agcggggccg acggtctcga cgaggcgttc gcggcggcgg
    10861 gggcgctggt cagcagcgtc gtcgtctaca gctggggcgc ctcggatcct gacgtcctcg
    10921 cagcatcggt gggacgcacc gcgcagggtc tgcacgacgc cgtcgtcttc acctcggcgc
    10981 cgggggttca cgcgtggctc gacacggcat ccgaggagca gctcgacgcg atccgcgaac
    11041 gcaacgcgag cggccagctc gtgctggcga ccgtcggccc gatcacggcg gcgcccctgg
    11101 ccgctgtcgg actggtcccc ctcgtccccg gtcgatggcg cctgggcgcg gtggtccgcg
    11161 agctggtgac ccattacgcg cagcagccca cgctcgagac cgtggccggg ccgctcgccg
    11221 tgcgtgctcg cggtgcggtg ctcgacgggc gggcactgac cctcaccccg gccggtgcgg
    11281 acatcctcgc cgcgctcgct cgtgccgagg gcgcggtcgt tcctcgggag cggctgcagg
    11341 cgctgctgcc gggtgggtcg agcgacggcc acgcggtcga ggcggccatc gcccgcctgc
    11401 gcgagtgctg tggctccaag gacgtcgtgc gcactgtcgt caagcgcggc tacgccctgg
    11461 ccctggtgca tccgtcgtga gcgcccccct catcgtgtgc gcccatggca cccgtgaccc
    11521 cgcagggcga gggacgatcg ccgcggtcgt cgccgccgtg gcggcgcggc tccccgacgt
    11581 ggcggtgctc gaggcctatg tcgacgtcca cgggccggac gtcgtcgagg tcgtcgacgg
    11641 tctgccggcg ggggagggag gcgtggtcgt gcccctcctg ctcgccgggg gttaccacgt
    11701 gcacgtcgac atcgcgcagg ccgtggcccg ccggccggac gtggtcgcca cgcctgccct
    11761 cgggcccgac gaccggctcg tcgacatcgt cctggaccga ctgcggtcgt ccggggcgac
    11821 cgcggggatg ccggtggtcc tcgtccccgc agggtccagc gacgcgcgcg cccaggcgga
    11881 cagcgcggcc gtggccgagg ccctcgcgcg ccggtggggc gggccggtga ccctggggtt
    11941 cgctgcgggc ccgcagccgt ccgcagcgga tgccgtggtg ctggctcgcg agtcgagcgg
    12001 cggccgggtg gcgctcgcct cgtacctgtt ggccccgggc ttcttccagc gccggctcga
    12061 ggatgcgggt gccgctgtcg tgaccgggcc gctcgcgccg gacgagcgga tcgtcgacat
    12121 cgtggtcgac cgctaccgcg tcgccacgtc ctgaccgtcg ggacgacggg gcgacggggc
    12181 ggggatcagc cccagccgag ggctcgcagg atcgcggcga gcgcgcccgc ggccaggacg
    12241 atgacgagga agttcacgcg cagcagcagc aggacgatcg ccagtcccgc tgcggcgaga
    12301 cgtgcgtcga cgacgatggc gccggcatcc ccggtcaccg actgcaccac cacgagcgcc
    12361 gcgagcagcg cgacgggcag cagggtcgtg acgcgccggg tgcgctcacc ggccatgacg
    12421 ctctcgggca cgaggtaccc cgcgagcttg accgcgacgg acagcaggct cgcgatcagg
    12481 acggc
//
LOCUS       DAOSOC010000062        12343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5116_length_12343_cov_7.960856, whole genome shotgun sequence.
ACCESSION   DAOSOC010000062 DAOSOC010000000
VERSION     DAOSOC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5116_length_12343_cov_7.960856"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1885
                     /locus_tag="PL014_06430"
     CDS             <1..>1885
                     /locus_tag="PL014_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56763.1"
                     /translation="GLARVEDVLAELGEPLTSTPDEPLPWDPAVGPLLTEVVRELDAR
                     VTATMTAFDAASSAERAAAESLAAGRASAALRERGARARTRLEELAANASAHEQRTRD
                     LDAAERAATVAGHLAAADRAQDEVQVARAAVESTGRALAPSPLAQANDAEVDAALEQV
                     RDLDATVTELARHEQDAAARSARRALLRSRLADAEGQHAAAQASVLAAQAEADDLAEQ
                     VDLRAAGAARIAELELLVATTAERLALVDAAEDDHRRAAELAPRRSSLHEALLDARTE
                     LLDLRQRRLDGMAAELAAGLADGDPCPVCGGAEHPHLAVATDPVSGDQITAAEHRVDQ
                     ARSLLTAIEREIEVLTASAASRLAGLQGATRASLETKQSQRRAELAAAHADAQEHSVL
                     LARLAAVRAALQTHEDTLTTLLAQTRSTTAVLGELDDQDAASATRAEELRAAHELCPC
                     HGGGTGAHAAFARLLGDHRAARTSLAESVTRAAVASEVVDDALTTAGLRDTAHVRAAL
                     RPAAEVAALRVEVAHHEEESAAARATLDDPQVAAALCGDEIDIEALREAEAAARAALL
                     QAQTTRDDAARGLRLLERIVPEVLAAGAAVSTAQDHDQRVRDLADTTSGTGPENTLRM
                     RLTS"
     assembly_gap    1886..1985
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1986..2222
                     /locus_tag="PL014_06435"
     CDS             <1986..2222
                     /locus_tag="PL014_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56764.1"
                     /translation="FDLGTLFIDEGFGSLDDDSLEQVLTVLDGLRAGGRAVGVVSHVA
                     DLRSRITHQAVVHKHASGSTVDVRVGTRSDQPAA"
     gene            complement(2251..5133)
                     /gene="gcvP"
                     /locus_tag="PL014_06440"
     CDS             complement(2251..5133)
                     /gene="gcvP"
                     /locus_tag="PL014_06440"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOT56765.1"
                     /translation="MSTTSADLPAFAGRHIGPRDEDIATMLSVVGRTSLDDLLDAAVP
                     DGIRALEALDIEPAASEAAVVAELREVAARNRRVTSMIGLGYYGTHTPAVIARNVLEN
                     PAWYTAYTPYQPEISQGRLEALLNFQTVVSDLTGLPIAGASLLDEATAAAEAMTLMRR
                     SSKAPADAVLLVDTEVFPQTLGVVRTRAVPLQIPVVVADLSGVTSTEGLRDAAGGAEV
                     FGVLVQYPAMDGILHDLSALTAAAHEVGALVTASADLLALTLATPPGEWGADVAVGTS
                     QRFGVAMGFGGPHAGYMSVRPGLERTMPGRLVGVSVDAVGAPAYRLALQTREQHIRRE
                     KATSNICTAQVLLAVMASMYAVYHGPAGLRAIALGVHAHAEALRGALRDGGVDVAEGP
                     IFDTLRVHVPGRAREVVDAALAAGVNLWLHDADTVQLSVDETTGLVDLHRVAKAFGVA
                     APEQVDIATPQWDQALARTSDYLTHPVFSTHHSETSMLRYLRRLSDRDFALDRGMIPL
                     GSCTMKLNATTEMVAVTWPEFAEVHPFAPHEQTRGIRGLVDQLSGWLCDITGYDAVSL
                     QPNAGSQGELAGLLAIAAYHRSRGDDERTVCLIPASAHGTNAASAVMAGLKVVVVKTD
                     PITGNVDMDDLKAKVEAHRETLAAIMVTYPSTHGVFEDTITDLCAMVHDAGGQVYVDG
                     ANLNALVGVARPGKFGADVSHLNLHKTFCIPHGGGGPGVGPVGVRAHLAPHLPNHPLD
                     ELAGPTDGVGPVSAAPYGSASILPISWAYVRLMGGAGLRRATEVAVLNANYVAARLRE
                     HYPVLYSGTDGLVAHECILDLRGITKDTGVSVDDVAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESEDLAELDRFCDTMIQIRREIDEVGSGAVAAADSVLRGAPHTAEAIAGDWDHG
                     YDRRTAAFPEGVDPTSKYWPPVRRIDGAYGDRHLVCSCPPPEAFEE"
     regulatory      complement(5209..5304)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(5418..5999)
                     /locus_tag="PL014_06445"
     CDS             complement(5418..5999)
                     /locus_tag="PL014_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT56766.1"
                     /translation="MVSTGEARESGASPVPAQGVLFADDLPELDDEIGYRGPTACRAA
                     GITYRQLDYWARTGLLEPSVRAAGGSGTQRLYGFRDILVLKIVKRLLDTGVSLQQIRI
                     AISVLRSRGVDDLAQITLMSDGASVYECTSNDEVIDLLQGGQGVFGIAVGKVWREVEG
                     ELASLPSERAEHDVAAAPRPGDELGARRAQRLA"
     gene            complement(6177..6668)
                     /locus_tag="PL014_06450"
     CDS             complement(6177..6668)
                     /locus_tag="PL014_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOT56767.1"
                     /translation="MKVLDVLGVRVEMPTNQPIVLLRERDGDRYLPIWIGAAEATAIA
                     YAQQGVVPPRPLTHDLLRNVIDELGHTLTRVRIVALKDGVFHAVLDLDGGAQAGGTDI
                     ESRSSDAIALALRAGAEIVAEEDLLDEAGIEMAQTEDDEVEKFKAFLDHVSADDFDTE
                     GES"
     gene            complement(6694..7353)
                     /locus_tag="PL014_06455"
     CDS             complement(6694..7353)
                     /locus_tag="PL014_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT56768.1"
                     /translation="MVRALADEFPDVTISKVRFLEAEGLVTPSRTASGYRKFTDGDVE
                     RLRYVLRAQRDRFWPLRVIKDNLDALDRGLTLAPGHGGRPAAPDLPPDPDVPEVSELA
                     VGTTLRLTPAELAGATGLAPAAVADLVTHGLLRPGSDGLHDEADLAVAHAAAGLAGYG
                     VEPRHLRGFRAAADREVGLVEQVVGARRGQDATRSAAEVAHLCLRLHAALVRGGLAEG
                     H"
     gene            complement(7403..7813)
                     /locus_tag="PL014_06460"
     CDS             complement(7403..7813)
                     /locus_tag="PL014_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT56769.1"
                     /translation="MRLAAVQAETSPRVEEPVHESEHVLTKADLATVEALRPGTALLV
                     VLRGPNTGARFLLDDDEVVSGRHPDSDIFLDDVTVSRRHAIFRRTPEGFVVRDVGSLN
                     GTYVNRELVEAATLTTGDEVQIGKFRLVFYASQA"
     assembly_gap    7850..7949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7950..8215)
                     /locus_tag="PL014_06465"
     CDS             complement(<7950..8215)
                     /locus_tag="PL014_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HOT56770.1"
                     /translation="MSDLEYPAGLRYTAEHEWVSSTGDTLRVGITSFAQEALGDVVYV
                     SLPAVGETVAAGGTCGEVESTKSVSDLYAPVSGEITAVNELLDAT"
     gene            complement(8250..9044)
                     /locus_tag="PL014_06470"
     CDS             complement(8250..9044)
                     /locus_tag="PL014_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOT56771.1"
                     /translation="MSEQPSGESGERPAESGGRPAANPPRAWRRLLRAGSPRATRANV
                     LAMVLAAALGFAIIAQVRQTSIQGLENLREDELVRIFAGVDQDGDRLADEIRGLENSL
                     ELLQSQSTNGEEAQRAARERLDALGILAGTAPAKGPGIVLTITGVDGGVAAPIILDTV
                     QELRAAGAEAIQVGDERVVANTWISERDGDLVISGKVVAPPYTIRAIGAANDLAGAME
                     IPGGVTATVRRAEGKTKTEIRDEVVVDALLTLAPPQYARPVPTTTP"
     gene            complement(9041..9373)
                     /locus_tag="PL014_06475"
     CDS             complement(9041..9373)
                     /locus_tag="PL014_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HOT56772.1"
                     /translation="MIPAIGLALGILIGLLLQPAVPVWLQPYLPIAVIAALDAVFGAV
                     RAILDGIFDDKVFVVSFLSNVVVAAFIVFLGDKLGVGAQLSTGVVVVLGVRIFSNVAS
                     IRRHLFNA"
     gene            complement(9370..10311)
                     /locus_tag="PL014_06480"
     CDS             complement(9370..10311)
                     /locus_tag="PL014_06480"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOT56773.1"
                     /translation="MSVPPTDTTPSPPPEASPPNQRTRAPDESMSLLTTMLQRPLDPG
                     YAAAADRREAAGMPRATSLRAPRLILAMVLLGLMVGVAASNLTASDSPRAQARKDLIA
                     QIEDRRAQVDVLTARATQLQTEVADLEAQQFGAVGDLERSRELAATVGAVELQGPGFT
                     ITMDDAPGSSADEADPNNPEAEQGRVYAKDLRFVVNALWQAGAEAVSINGNRLTSLSA
                     IRFAGPALIVDNRPLTRPYLITALGDPARFPADFADGEGGSYLSTLRGSFGVRVDTKV
                     SKELTVPAAVGLNLLYARTGDTGDSASSTATPERSSS"
     gene            complement(10308..10904)
                     /locus_tag="PL014_06485"
     CDS             complement(10308..10904)
                     /locus_tag="PL014_06485"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT56774.1"
                     /translation="MATGGPDEQVSNRVLTVPNALSALRLVGVPVFLWAILSERDGLA
                     IVLLMASGLTDYLDGKIARTYGLVSRVGQLLDPIADRLYIVSTLLGLAWREIIPWWLV
                     AVLFAREAFMAVVVLIAKRHGWVGLPVHFAGKAATFNLLYAFPLLLLADGDGTLSRIA
                     EPVGWAFAWWGTALYWVAGILYALQLRALLQARRVDAA"
     gene            10961..12112
                     /locus_tag="PL014_06490"
     CDS             10961..12112
                     /locus_tag="PL014_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT56775.1"
                     /translation="MSAPLLRGLASDHRRDRTSVKWVAYPPDVLPLWVAEMDAAPCPA
                     VVDAVTSAMRRGDTGYGWGPRYAEAVARYADATWGWQVDTDASMIVPDVMIGASEVLR
                     LLTDEGGPVVISSPTYNAFYGFLDAIGRRTVDAPLTGDGRLDPEALRTAFRHATARGE
                     RAAYLLCNPQNPTGTVHSRDELTTLAALAEEFGVRVVADEIHAPIVFPGGPAFTPFLT
                     VPGGERGFSVFSPSKGWNLAGLKSALVMAGPDATDDLARLHEVHTHGSSHIGAIAHVA
                     ALDDGRQWLAQLLLELDANRTLLARLLGEHLPAIRWRAPDATYLAWLDCRDLGLGDDP
                     SSVFLRRGRVALGSGPQYGPQSGRGFVRLNLATSPEIIEDAVHRMAAAL"
     gene            complement(12158..>12343)
                     /locus_tag="PL014_06495"
     CDS             complement(12158..>12343)
                     /locus_tag="PL014_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT56776.1"
                     /translation="GQRWWVEDLQSSNGTYVGPASGPLPVDPIAPGTRHEFSEDDRVY
                     VGAWTRLVVRRATPEEQ"
BASE COUNT         1753 a         4346 c         4378 g         1666 t
ORIGIN      
        1 ccgggctggc ccgggtcgag gacgtcctgg cggagctcgg cgaaccgctg acctcaaccc
       61 ccgatgagcc actgccctgg gaccctgcgg tggggccact gctgaccgag gtcgtgcgtg
      121 agctggacgc ccgagtgacc gcgacgatga ccgccttcga cgctgcctcg agcgctgagc
      181 gtgccgctgc ggagtcgttg gcggccggtc gcgcctccgc ggccctgcgc gagcgcggag
      241 cccgggcccg caccaggctc gaggagctgg cggcgaacgc ctccgcgcac gagcagcgca
      301 cccgtgacct ggatgccgct gagcgggccg ccaccgtggc cggccacctg gcggccgccg
      361 accgcgcgca ggacgaggtg caggtcgcgc gagcggcagt cgagtccacc ggacgagccc
      421 tcgccccgag tcccctcgcg caggcgaatg acgccgaggt cgatgccgca ctggagcagg
      481 tgcgcgacct cgacgccacc gtgaccgagc tcgcccggca cgagcaggac gcggctgcgc
      541 ggtcggcccg gcgggccctg ctgcgctccc gcctcgccga cgccgagggc cagcacgcgg
      601 cggctcaggc cagcgtgctt gcggcccagg ccgaggccga tgacttggcc gaacaggtcg
      661 acctgcgcgc cgccggtgca gcccggatcg ccgagctcga gcttctcgtc gccaccaccg
      721 ccgagcgcct ggccctggtc gacgccgccg aggacgacca ccgtcgggca gccgagctgg
      781 caccgcgccg gagcagcctg cacgaggccc tgctcgatgc ccgaaccgag ctgctcgacc
      841 tgcgtcagcg ccggctcgac ggcatggctg ccgagctggc cgccggcctc gccgacggcg
      901 atccgtgccc ggtgtgcggc ggcgctgagc acccccacct cgccgtcgcc accgaccccg
      961 tctcgggtga ccagatcacg gctgccgagc accgggtcga ccaggctcgc agcctgctga
     1021 ccgccatcga gcgcgagatc gaggtgctca ccgcctcggc cgcgtcccgg ctcgcaggtc
     1081 tgcagggcgc gacccgagcc tcgctggaga cgaagcagtc gcagcgccgc gccgagctcg
     1141 ccgcagcgca cgccgacgcg caggaacact cggtgctcct cgcccgcctc gccgcggtcc
     1201 gcgccgctct gcagacccac gaggacacgc tcacgaccct gctggcgcag acgaggtcga
     1261 cgacggccgt cctcggcgag ctcgacgacc aggatgccgc ctcggcaacg cgcgccgagg
     1321 agcttcgcgc agcccacgag ctgtgcccct gccacggtgg tggcacggga gcacacgcag
     1381 ccttcgcccg gctgctcggc gaccaccgcg cagcccgcac gtcgctcgcc gagtccgtca
     1441 cccgggcggc agtggcctcg gaggtcgtcg atgacgcgct caccaccgcg gggttgcgcg
     1501 acaccgcgca cgtgcgggcg gccctgcgtc cggcggcgga ggtggcggcc ctgcgtgtcg
     1561 aggtcgcgca ccacgaggag gagtcggccg ccgcgcgagc caccctcgac gatccgcagg
     1621 tcgctgcggc gctctgcggt gacgagatcg acatcgaggc cctgcgcgag gcggaagccg
     1681 cagcccgagc agccctgctc caggcccaga cgacccgtga cgacgcggcc cgcgggttgc
     1741 gcctgctcga acgcattgtc cccgaggtcc tcgcggccgg agccgccgtg tccaccgcgc
     1801 aggaccacga ccagcgcgtg cgcgacctcg ccgacacgac gagtggcacc gggccggaga
     1861 acacgctgcg catgcgcctc acgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnttcga cctcggcacc ctgttcatcg acgagggctt cggcagcctc gacgacgaca
     2041 gcctcgaaca ggtgctgacc gtgctcgacg gcctgcgcgc gggcggccgc gcggtcggcg
     2101 tcgtgagtca cgtcgccgac ctgcgcagcc gcatcaccca ccaggcggtg gtccacaagc
     2161 acgcgtcggg cagcacggtc gacgtccggg tgggaacccg cagcgaccaa ccggccgcct
     2221 gaccactact ccccacccgg ccgcctgccc ctactcctcg aacgcctccg gcggcgggca
     2281 ggaacacacc aggtgccggt cgccgtacgc gccgtcgatc cggcgcaccg gaggccagta
     2341 cttggaggtc gggtcgaccc cctccggaaa ggcggccgtc cggcggtcgt acccgtggtc
     2401 ccagtcgccg gcgatggctt cagccgtgtg gggcgccccg cgcagcacgc tgtccgccgc
     2461 ggcgacggct cctgagccga cctcgtcgat ctcccggcgg atctggatca tggtgtcgca
     2521 gaaccggtcg agctcggcga ggtcctcact ctccgtcggc tcgaccatga gggtgccagc
     2581 cacggggaac gacatcgtcg gggcgtggaa gccgtagtcg atcagtcgct tggcgacgtc
     2641 gtcgacgctg actcccgtgt ccttcgtgat gccacgcagg tcgaggatgc actcgtgggc
     2701 gaccagcccg tcggtgcccg agtacagcac cgggtagtgc tcacgcagtc gggcggccac
     2761 gtagttggcg ttgagcaccg ccacctcggt ggcgcgacgc aaccccgccc cgcccatgag
     2821 gcgcacgtac gcccacgaga tgggcaggat gctcgccgag ccgtaggggg cagcagacac
     2881 cgggcccacg ccgtcggtcg ggccggccag ctcgtcgagc gggtggttgg gcaggtgcgg
     2941 cgccaggtgg gcgcgcacgc cgaccggacc gacaccgggc ccgccgccac cgtgcgggat
     3001 gcagaacgtc ttgtgcaggt tcaggtgcga gacgtcggca ccgaacttcc ccggacgggc
     3061 gacgccgacg agggcgttga ggttggcacc gtcgacgtag acctgaccgc cagcgtcgtg
     3121 gaccatcgcg cacaggtcgg tgatggtgtc ctcgaagacc ccgtgggtcg acggataggt
     3181 gaccatgatc gccgcgaggg tctcacggtg cgcctcgacc ttggccttga ggtcgtccat
     3241 gtcgacgttg ccggtgatgg ggtcggtctt cacgacgacg accttcaggc ccgccatgac
     3301 cgcactggcc gcgttggtgc cgtgggcgct ggccgggatg aggcacacgg tgcgctcgtc
     3361 gtcgccccgc gagcggtggt aggccgcgat cgcgagcagc cctgcgagct caccctgcga
     3421 gccggcgttc ggctggagcg agaccgcgtc gtagccggtg atgtcgcaca gccagccact
     3481 cagttggtcg acgaggccgc ggatgccgcg ggtctgctcg tgcggcgcga aggggtggac
     3541 ctcggcgaac tcgggccagg tcaccgcgac catctcggtc gtggcgttga gcttcatcgt
     3601 gcacgacccc agcgggatca tcccgcggtc gagggcgaag tcgcggtcgg acaaccgccg
     3661 caggtaccgc agcatcgacg tctcgctgtg gtgggtcgag aacacggggt gggtgaggta
     3721 gtcgctcgtg cgggccaggg cctggtccca ctgcggcgtc gcgatgtcca cctgctccgg
     3781 cgcagcgacg ccgaaggcct tggccacccg gtgcaggtcg accagtccag tcgtctcgtc
     3841 gacgctgagc tggacggtgt cggcgtcgtg cagccagagg ttcacgccgg ccgccagggc
     3901 cgcatcgacc acctcgcggg cccgccccgg gacgtgcacc cgcagcgtgt cgaagatcgg
     3961 cccctcggcc acgtcgacgc caccgtcacg aagggcgcca cgcagggcct cggcgtgggc
     4021 gtggacaccg agcgcgatgg ctcgcagacc cgcagggccg tggtagaccg cgtacatgct
     4081 ggccatgacg gcgagcagca cctgggccgt gcagatgttc gacgtcgcct tctcgcggcg
     4141 gatgtgctgc tcgcgcgtct gcagggcgag ccggtaggca ggggcgccca cggcatcgac
     4201 cgagaccccg accaggcgcc cgggcatggt gcgctccagg ccggggcgca cgctcatgta
     4261 cccggcgtgg gggccaccga agcccatcgc gacgccgaag cgctggctcg tgccgacggc
     4321 gacgtcggca ccccactccc ccggcggcgt cgccagcgtc agggcgagca ggtcggcgct
     4381 cgcggtgacg agcgcgccca cctcgtgggc cgccgcggtc agggccgaca ggtcgtggag
     4441 gatgccgtcc atcgcggggt actgcaccag gacgccgaac acctcggccc cgcccgcggc
     4501 atcccgaagg ccttcggtgc tggtgacacc cgagaggtcg gcgacgacca ccgggatctg
     4561 caggggaacg gcccgggtgc gaacgacacc cagcgtctgc gggaagacct cggtgtcgac
     4621 gaggagcacg gcatccgccg gcgccttcga ggatcgccgc atgagcgtca tcgcctcggc
     4681 cgccgcggtc gcttcgtcga gcagcgacgc accggcgatc gggagaccgg tgaggtcgct
     4741 gacgacggtc tggaagttca gcagcgcctc gagccggccc tgcgagatct ccggctggta
     4801 gggcgtgtag gcggtgtacc aggccgggtt ctcgagcacg ttgcgcgcga tgaccgccgg
     4861 ggtgtgggtg ccgtagtacc cgagcccgat catcgaggtg acccgtcggt tgcgggccgc
     4921 gacctcgcgc agctcggcga ccaccgcggc ctcggacgcc gcgggctcga tgtcgagcgc
     4981 ctcgagagcg cggatgccgt ccggcaccgc ggcatccagc aggtcgtcga gggaggtgcg
     5041 gccgacgacg gagagcatcg tcgcgatgtc ctcgtcacgg gggccgatgt ggcgaccggc
     5101 gaaggcgggc aggtcggctg aggtggtgct catggcgctc ctgaggcaga cggcaaggac
     5161 gtctccccct ctgtcaccac cggggtggcc ggtgagctcc agaggtgcct gtcccgtgtg
     5221 gtcccttgcg cctgagaggt tccggggagg tttgcccctt cggcgccccg tcaacagcga
     5281 cgaggactct cccacacggt gtgagcggca cggtcaacgt accaccgccg cacccttccc
     5341 acccctggtg cgtgacacac ctgccgagcg gcgtgtcacg tggcacgcgg tacgcggcgg
     5401 aaaagtcggg acgtgcctca ggccaggcgc tgggcgcggc gggcgccgag ctcgtcaccg
     5461 ggccggggcg cggcggcgac gtcgtgctcg gcccgctcac tgggcaggct ggcgagctca
     5521 ccctcgacct cacgccagac cttgccgacg gcgatgccga agactccctg accgccctgc
     5581 agcaggtcga tgacctcgtc gttggaggtg cactcgtaga ccgaggcccc gtcgctcatc
     5641 aaggtgatct gcgcgaggtc gtcgacgccc cgggagcgga gcacgctgat ggcgatccgg
     5701 atctgctgca acgagacacc ggtgtcgagc agcctcttga cgatcttgag cacgaggatg
     5761 tcgcggaagc cgtagaggcg ctgggtcccg gagccgcccg cggctcgaac cgagggctcc
     5821 agcagaccgg tgcgcgccca gtagtcgagc tgacggtagg tgatgccggc cgcccggcac
     5881 gcggtgggcc cgcggtaacc gatctcgtcg tcgagctcgg ggaggtcgtc ggcgaacagc
     5941 acgccctgtg cagggacggg actggccccg gactctcgcg cttctcctgt ggagaccacg
     6001 ccatacctcc tcgactcgtc ttcgcagacg actgtacgcg ccgaaatgac atcagtgtca
     6061 ccctggcagt cacggatgtg acaggtgtga cggtaggggc aggtgtgact caggtcaacg
     6121 accgctgctc gagcgtgctc cccgggcgtg tcgcgaggaa gcgctcgtcg ctgcgctcag
     6181 ctctcgccct cggtgtcgaa gtcatcggcc gagacgtggt cgaggaaggc cttgaacttc
     6241 tccacctcgt cgtcctccgt ctgggccatc tcgatgcccg cctcgtcgag gaggtcctcc
     6301 tcggcgacga tctcggcacc agcacgcagc gccagggcga tcgcgtccga ggaccgcgac
     6361 tcgatgtcgg tgccgcccgc ctgggcgcca ccgtcgaggt cgagcacggc gtggaagacc
     6421 ccgtccttga gggcgacgat gcgcacccgg gtcagggtgt gaccgagctc gtcgatgacg
     6481 ttgcgcagca ggtcgtgggt gaggggccgc ggcggcacga ctccctgttg ggcgtaggcg
     6541 atcgcggtcg cctcggcggc accgatccag atcggcaggt aacggtcgcc gtcacgctct
     6601 cgcaggagca cgatcggctg gttggtgggc atctccacac ggactccgag cacgtccaag
     6661 accttcaccc tgccaccgta cctcccccga cggtcagtgg ccctcggcca gaccgccgcg
     6721 caccagggcc gcgtgcaacc tcaggcagag gtgggccacc tcggcagcgc tgcgcgtggc
     6781 gtcctgcccc cgccgcgcac cgacgacctg ctcgaccagc ccgacctcgc gatcggcagc
     6841 cgcccggaac ccgcgcaggt gacgaggctc gacgccgtac ccggccaggc ccgcggccgc
     6901 atgggcgaca gctaggtccg cctcgtcgtg cagcccatcg gagccgggcc gcagcaggcc
     6961 gtgggtcacg aggtcggcga cggccgccgg cgccagcccg gtcgccccgg ccagttccgc
     7021 gggcgtgagg cgcagcgtcg tgccgacggc gagctccgag acctccggga cgtcggggtc
     7081 gggagggagg tccggagccg ctggtcgacc gccatgcccc ggagccaggg tcaggccgcg
     7141 gtccaacgcg tcgaggttgt ccttgatgac gcgcagcggc cagaaccggt cacgctgggc
     7201 ccgcaggacg taccgcaacc gctccacgtc gccgtcggtg aacttccggt acccggatgc
     7261 cgtacgggag ggagtgacca gcccctcggc ctcgaggaaa cggaccttgg agatcgtgac
     7321 gtcgggaaac tcgtccgcca acgcccgaac caccgccccg accgtgagga ggcgaggggg
     7381 cgccgactcg gccacgggtg ggtcaggcct ggctcgcgta gaagacgagg cggaacttgc
     7441 cgatctggac ctcgtcaccc gtcgtcaggg tggccgcctc gacgagctcg cggttgacgt
     7501 aggtgccgtt gagcgagccg acgtcgcgca cgacgaatcc ctcgggcgtg cgccggaaga
     7561 tcgcgtgacg acgcgagacg gtcacgtcgt cgaggaagat gtcgctgtcg gggtgacgcc
     7621 cggagacgac ctcgtcgtca tcgaggagga accgggctcc ggtgttgggc ccgcgcagca
     7681 cgacgagcag ggccgtcccg ggccgcagcg cctcgacggt tgcgagatcg gccttcgtga
     7741 ggacgtgctc gctctcgtgg accggctcct cgacacgcgg gctcgtctcc gcctgtaccg
     7801 cggccagacg catcgtcgtc gactcgggca cggggcctcg accccccacn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tggcgtccag cagctcgttc acggcggtga
     7981 tctcaccgga gaccggggcg tagaggtcgc tcacggactt cgtggactcc acctcgccgc
     8041 aggtgccgcc ggcggcaacc gtctcgccga ccgccggcag gctcacgtag acgacgtctc
     8101 cgagcgcctc ctgcgcgaag gaggtgatgc cgacgcgcag ggtgtcgccg gtgctgctga
     8161 cccactcgtg ctcagcggtg tagcgcagtc cggcggggta ctcgaggtcg ctcatgactc
     8221 tcctaggtgt ggcccgcgag cgggagtgat cagggggtgg tggtcggtac gggccgagcg
     8281 tactgaggcg gggcgagggt cagcaacgca tcgaccacta cctcgtcacg gatctcggtc
     8341 ttggtcttgc cctcggcgcg gcgaacggtg gcggtcacac cgccggggat ctccatcgca
     8401 ccggcaaggt cgtttgcggc cccgatggcg cggatcgtgt acggcggggc cacgaccttc
     8461 ccggagatga cgaggtcacc gtcgcgctcg gagatccacg tgttcgccac gacgcgctcg
     8521 tcgccgacct ggatggcttc ggccccggcg gcccgcagct cctggacggt gtcgaggatg
     8581 atgggggccg cgacgccgcc gtcgactccg gtgatggtga gcacgatgcc gggccccttg
     8641 gccggggcgg tgccggcgag gataccgagg gcgtcgagac gctcccgggc ggcacgctgg
     8701 gcctcctccc cgttcgtgga ctggctctgg agcagctcca gtgagttctc cagccctcgg
     8761 atctcgtcgg ccagtcggtc gccgtcctgg tcgacccccg cgaagatgcg caccagttcg
     8821 tcctcgcgca ggttctccag cccttggatg gaggtctggc gcacctgggc gatgatcgcg
     8881 aatcccagtg ccgcggccag gaccatcgcg aggacgtttg cccgggtcgc gcggggtgag
     8941 ccggcgcgca ggaggcggcg ccacgctcga ggcggattcg cggccgggcg tccgcctgac
     9001 tcggccgggc gttccccgga ctcaccagag ggctgctcgc tcatgcgttg aagaggtggc
     9061 ggcggatcga cgccacgttc gagaagatgc gcaccccgag gacgacgacg acgccggtgg
     9121 aaagctgggc tccgacgccg agcttgtcgc ccaggaagac gatgaaggcc gcgacgacca
     9181 cgttggacag gaaggagacg acgaacacct tgtcgtcgaa gatcccgtcc aggatggccc
     9241 gcacggcacc gaagaccgca tccagtgccg cgatgacggc gatgggtaga tagggctgca
     9301 gccacacggg caccgcgggc tgaaggagca gcccgatgag gatgccgaga gccagaccga
     9361 tggcggggat cacgacgagc tcctctcagg ggtggcggtg gatgacgcgg agtctccagt
     9421 atcccccgtc ctcgcgtaga gcaggttcag acccaccgcg gcgggcacgg tcagctcctt
     9481 cgacaccttg gtgtcgaccc ggacaccgaa gctgccccgc agggtcgaca ggtagctgcc
     9541 cccctcaccg tcggcgaagt ccgcaggaaa gcgggccgga tccccgaggg cggtgatgag
     9601 gtagggccgg gtcagcggac ggttgtcgac gatgagcgcc ggcccggcga agcggatggc
     9661 cgacagcgac gtgaggcggt tgccgttgat gctgacggcc tcggccccgg cctgccagag
     9721 ggcgttgacg acgaagcgaa ggtccttggc gtagacgcgg ccctgttcgg cctccgggtt
     9781 gttggggtcg gcctcgtcag cactcgaccc gggggcgtcg tccatggtga tggtgaagcc
     9841 cgggccctgc agctcgacgg ccccgacggt ggccgcgagc tcccggctgc gctcgaggtc
     9901 ccccacggcc ccgaactgct gggcctcgag gtcggccacc tccgtctgca gctgcgtggc
     9961 acgtgcggtc agcacgtcga cctgggcgcg gcggtcctcg atctgggcga tgaggtcctt
    10021 gcgggcctgt gctcgcgggg agtccgaggc ggtgaggttg gaggccgcca ctcccaccat
    10081 cagaccgagc aggaccatgg cgaggatgag gcgcggggcg cgcagtgacg tcgcgcgcgg
    10141 catccccgct gcctcacggc gatccgcggc agcggcgtac ccggggtcga gcggtcgctg
    10201 gagcatcgtc gtcagcaggc tcatcgactc gtcgggcgct cgcgtgcgct ggttcggcgg
    10261 tgacgcctcg ggcggaggcg acggcgtggt gtcggtgggc ggcacgctca cgccgcgtcc
    10321 acccgccggg cctgcagcag ggcgcgcagt tgtagggcgt agaggatgcc ggcgacccag
    10381 tacaacgccg tgccccacca ggcgaacgcc cagccgaccg gttccgcgat gcgcgacagc
    10441 gtgccgtccc cgtcagcgag gagcagcagc gggaaggcgt agaggaggtt gaacgtcgct
    10501 gccttgccgg cgaagtgcac cggaagcccc acccagccgt gtcgcttggc gatcagcacg
    10561 acgacggcca tgaaggcctc gcgggcgaag agcaccgcca cgagccacca cgggatgatc
    10621 tcgcgccagg ccagtccgag cagcgtggac acgatgtaga gacggtcggc gatcgggtcg
    10681 aggagctgac cgacacgcga gaccagtccg taggttcggg cgatcttgcc gtcgaggtag
    10741 tcggtgagcc ccgaggccat gagcagcacg atggccagac cgtcacgctc ggagaggatg
    10801 gcccagagga agaccggaac cccgaccagc cgaagcgccg agagcgcgtt cggcacggtg
    10861 agcacccgat tgctcacctg ctcatcgggc ccgcccgtcg ccatgtgcgc aactgtatgt
    10921 cgtgcgcggg tgctgcgccg cacccccata gggtgtcgcc atgtccgccc ccctcctgcg
    10981 tggcctcgcc tccgatcacc gccgtgaccg cacgagcgtg aagtgggtgg cctacccgcc
    11041 cgacgtgctg ccgctctggg tggccgagat ggacgccgca ccgtgccccg cggtcgtcga
    11101 tgcggtgacc tccgccatgc gccgcggtga caccgggtac ggctggggcc ctcgctacgc
    11161 cgaggcggtg gcccgctacg ccgacgccac gtggggctgg caggtcgaca ccgacgcctc
    11221 gatgatcgtg cccgacgtca tgatcggtgc cagcgaggtg ctgcgcctgc tgaccgacga
    11281 gggcgggccg gtcgtcatct cctcccccac gtacaacgcc ttctacggct tcctcgacgc
    11341 catcggccgt cgcaccgtcg acgcgccgct caccggtgac ggacgcctcg accccgaagc
    11401 cctgcgcacc gcgttccggc acgcgaccgc acgcggcgag cgggcggcat acctgctgtg
    11461 caacccgcag aaccccacgg gcaccgtcca ctcccgcgac gagctcacca ccctcgccgc
    11521 gctggccgag gagttcggcg tgcgggtcgt cgccgacgag atccacgccc cgatcgtctt
    11581 tcccggtggc cccgccttca cccccttcct caccgtgccg ggcggtgaac gaggattctc
    11641 ggtgttctcg ccgtccaagg gctggaacct cgccgggctg aagtcagccc tcgtcatggc
    11701 ggggccggac gcgaccgacg acctcgcccg cctccacgag gtgcacaccc acggctcgtc
    11761 ccacatcggg gcgatcgccc acgtcgccgc cctggacgac ggcaggcagt ggttggccca
    11821 gctgctcctc gagctcgacg ccaaccgcac cctcctggcc cggctcctcg gcgagcacct
    11881 acctgcgatc cggtggcggg ccccggacgc cacctacctc gcgtggctgg actgccgaga
    11941 cctcgggctc ggcgacgacc cgtcgtccgt gttcctccgg cgcggccggg tcgccctcgg
    12001 gtccgggcca cagtacggcc cacagagcgg ccgggggttc gtccggctca acctcgcgac
    12061 gtcgccggag atcatcgagg acgccgtgca ccggatggcc gcggcgctct gaaccctgtc
    12121 tcggcgctct gggccccgtt gctgcccctg agcccggcta ctgctcctcg ggggtcgcgc
    12181 gccgcacgac gagtcgcgtc caggcaccga cgtagacgcg gtcgtcctcg gagaactcgt
    12241 ggcgcgtgcc gggagcaatc gggtcgacgg gcagcggccc gctggcaggc ccgacgtacg
    12301 tgccgttgga ggactggagg tcctcgaccc accagcgctg acc
//
LOCUS       DAOSOC010000063        12295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5144_length_12295_cov_10.134559, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000063 DAOSOC010000000
VERSION     DAOSOC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_12295_cov_10.134559"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1058)
                     /gene="ppk2"
                     /locus_tag="PL014_06500"
     CDS             complement(138..1058)
                     /gene="ppk2"
                     /locus_tag="PL014_06500"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOT56777.1"
                     /translation="MNDDTRLSVTEDYAEEIEELHDLEEADGVEIDVKVGKTWQQGYP
                     YDKKLPRDEYERTKRKLQIEMLKLQLHAKETGAKVLVICEGRDAAGKGGAIKRFMEHL
                     NPRGARVVALEKPTEIERTQWYFQRYVQHLPSGGELVFMDRSWYNRAGVERVMGYCTP
                     TQYLEFMREVPDLERMLVNSGITLVKLWFSVSREEQAARFASRQNDPVRQWKLSPTDL
                     ASRDKWDAYTEAKEAMFFYTDTGDAPWTVIKSNDKKRARLEAFRVLLNRVDYAQKDAS
                     IVGQPDPLIVGPASKIFEAGEHTDRVLPRL"
     gene            complement(1112..1807)
                     /gene="phoU"
                     /locus_tag="PL014_06505"
     CDS             complement(1112..1807)
                     /gene="phoU"
                     /locus_tag="PL014_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOT56778.1"
                     /translation="MRKAFHEELDRVAETLVEMTHLASSAMNRATTALLDADIHLADS
                     VIAADAEIDALRADLDLLSFDLLARQQPVATDLRVIVTSMRMSSDLERMGDLARHVAK
                     VARLRYPASAVPAELRSTILQMGHVAERIVEKAGSVIAARDLEAAQQIERDDDAMDDL
                     HRELFARVTDPSWRHGTEAAIDITLVGRYYERFADHAVSVGHRVIWLVTGEFDPASEG
                     SHEDAEDGATVGA"
     gene            2002..3258
                     /locus_tag="PL014_06510"
     CDS             2002..3258
                     /locus_tag="PL014_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT56779.1"
                     /translation="MDPVLAAILGGAVGLVIGSVAVLATRWSERSTAAPEPVDPPLPR
                     GVGDVLSVLKSIAVVLDASDAVVNTSASAVSYGLVRHGELVHHELRHIARQVRRDGLI
                     REAEFDLPRGPNAQASTVMRVRVAPIAVSHVLILAEDHTQARRVEEVRRDFVANVSHE
                     LKTPVGGISLLAEAVLDARDDPDAVERFAERILVESARLTRLVKEIVDLSRLQVADTL
                     HEPVIVDVRAVAAEAVDRVRVAAEAHGIQLTLVGEEGLRVFGDAELLTTAIANLVTNA
                     VNYSGSGTHVGIGARRNGDSIEVSVTDQGEGIPAAEQERIFERFYRVDAARSRATGGT
                     GLGLAIVKHVCANHGGRVSVWSQEGRGSTFTMTLPAAADRPRVGTEQPHPQTLGSPEV
                     RDHADPSRTASDVEKERALHDAHPDR"
     gene            3236..3916
                     /locus_tag="PL014_06515"
     CDS             3236..3916
                     /locus_tag="PL014_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT56780.1"
                     /translation="MTRILIVEDEVSFSDPLSYLLSKEGYDVTVAENGHDALTAFDGA
                     GADLVLLDLMLPGLSGVDVCRALRQRSNVPVIMLTAKDSEIDKVVGLELGADDYVTKP
                     YSSRELLARIKAVLRRLAEPEELLPTTLEAGPVRMDVERHVVTVRGGQLPFPLKEFEL
                     LEMLLRNAGRVLTRGQLIDRVWGSDYVGDTKTLDVHIKRLRAKVEADPANPVHILTVR
                     GLGYKFEV"
     gene            complement(4001..4465)
                     /locus_tag="PL014_06520"
     CDS             complement(4001..4465)
                     /locus_tag="PL014_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56781.1"
                     /translation="MTTRAALALRATALLAASLLASGCATFSPTQTDYDYPASDGVAL
                     SIPGLELRNLALVVENSDGPAVLIGQAVNRGSSAVNVTFGVEGATSTETAAVPAFSGN
                     SLSDTGALIEFEGLPASAGDTITLTVSTPQAGQNVVTVPVLAGVHHNGTVFG"
     gene            4698..5180
                     /locus_tag="PL014_06525"
     CDS             4698..5180
                     /locus_tag="PL014_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOT56782.1"
                     /translation="MVFKVGETVVYPHHGAARIEEIKTRTIRGEEKLYLKLKVAQGDL
                     VIEVPAENCDLVGVRDVVSKEGLDKVFEVLRTPFAEEPTNWSRRFKANLEKLASGDVI
                     KVAEVVRDLWRRDKDRGLSAGEKRMLAKARQILVSELALAEKTDEEKAEVILDEVLAS
                     "
     gene            5210..5923
                     /gene="ispD"
                     /locus_tag="PL014_06530"
     CDS             5210..5923
                     /gene="ispD"
                     /locus_tag="PL014_06530"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOT56783.1"
                     /translation="MTDADAAPSSVGVVVVAAGSGSRLGADVPKAFVHLGGRPLLAHA
                     LDTIARMPGRTEVVVVAPAGLTDPDDPLWSGVDLPAGAVVVPGGAERTDSVAAGMAAL
                     PPGCEIVLVHDAARCLMPLAVFLRVVDAVRAGAAGAVPGLPVVDTIKTVDAAGVITGT
                     PERSALRAVQTPQGFRREVLERAHASGLQATDDAALVELCGDDVVVVDGDPLGAKVTT
                     ADDLERAQWLLRRAATLGG"
     gene            5920..6408
                     /gene="ispF"
                     /locus_tag="PL014_06535"
     CDS             5920..6408
                     /gene="ispF"
                     /locus_tag="PL014_06535"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOT56784.1"
                     /translation="MMQLPRVGVGVDVHALAPEGSDRELWVAGVYWPGERGLDGHSDA
                     DVAAHAACDALFTAAGIGDLGAHFGTGRPEFAGASGVTLLSEAARLVRAAGFEVGNIA
                     VQVIGNRPKVGTRRAEAERALSDAVDAPVSVSGTTTDGLGLTGRGEGIAAIATALVVA
                     AS"
     gene            complement(6486..7133)
                     /locus_tag="PL014_06540"
     CDS             complement(6486..7133)
                     /locus_tag="PL014_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT56785.1"
                     /translation="MPLAPSPGGVRVERVVTSGVFELDGGSWEVDNNVWLVGDDSDVL
                     VVDAAHDAPAILEAVGGRRVVAIVCTHGHNDHVNAVGDLVRATGATASLHPDDRVLWD
                     QSQTEPPGGELTDGQVFTVGDVELTVVHTPGHAPGACCLHAPALGVLLSGDTLFSGGP
                     GATGRSFSDFPTIIDSIRERLLTLPADTVVLTGHGDTTTIGDEAPHLQVWLDRGH"
     gene            complement(7148..8242)
                     /locus_tag="PL014_06545"
     CDS             complement(7148..8242)
                     /locus_tag="PL014_06545"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HOT56786.1"
                     /translation="MPQTVKGVIARSKGAEVELVDIVVPDPGPGEAVVKVESCGVCHT
                     DLHYREGGINDDYPFLLGHEAAGTVESVGEGVTEVAPGDFVILNWRAVCGQCRACLKG
                     KPWYCFATHNAKQKMTLTDGTELSPALGIGAFIDKTLVAAGQCTKVDRADAAAVGLLG
                     CGVMAGFGAAVNTGGVGRGDSIAVIGCGGVGNAAIAGAKVAGATTIIAVDVDARKLEA
                     AKAFGATHTVDSSTEDAVEAIRALTGGHGADVVVEAVGRPETYEQAFYARDLAGTVVL
                     VGVPTPDKQVTLPMIEIFGRGGSLKSSWYGDCLPSRDFPMLVELYRQGRFDLDAFVTE
                     RIGITDVEAAFDKMHRGEVLRSVVVMGEGQ"
     gene            complement(8448..9395)
                     /locus_tag="PL014_06550"
     CDS             complement(8448..9395)
                     /locus_tag="PL014_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOT56787.1"
                     /translation="MSDRLLALAAANNGIIASQDVHHADVDPHALAALLRAGVLRRVR
                     RGSFVVASDWAGATPEQQLALMTRAVLRTRGNAPGEAATHQSALAVHGLPLFGVPHDV
                     VDLFGLVRRVRREGALRVHAADPSLPVVDVDGCRSVTIAVALAQVALREGRLPVMVAA
                     DRALATGRVQIDDVVEMIGHLAPTARRGLRAQRWLRACDPLAESVGETRTRLLLIDLG
                     HAPRSQVRVTDAAGTVMARADFLVGSRVIVEFDGLVKYAGKDGREALAAEKRREDALR
                     ALGYEVVRLTWADLDHPRRVDALVRSALARASNRRVELT"
     gene            9713..11146
                     /gene="cysS"
                     /locus_tag="PL014_06555"
     CDS             9713..11146
                     /gene="cysS"
                     /locus_tag="PL014_06555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT56788.1"
                     /translation="MTLRLFDSLTRELRDFVPVVPGKAGIYICGLTTQAPPHLGHVRF
                     AVAFDVLRRWLARGHGYDVTLIRNVTDIDDKILAKSLESGEAWYAVSYRNELETSAAL
                     ASLGVLPPTYEPRATGHVPEMVELMAALVERGHAYAAADGSGDVYFDVRSWADYGALT
                     HQKVDDMVDAEDADPRGKRDPRDFALWKGHKADEPPTASWPTPFGPGRPGWHLECSAM
                     ARKYLGDTFDIHGGGVDLRFPHHENELAQSRAAGLGFANYWLHNAWVTLHGEKMSKSL
                     GNSLRVSEVVKEHRPLAVRYYLTAAHYRSTIEFHAGSLEESDTAVQRIEGFLRRALPG
                     ILTALPTGDETLPEDFREAMDDDLGVSSALAAVHEAVTTGNTALDDGDHEDAAAVARV
                     VIAMTDVLGVNPLDPAWSAGDSSAADALASLDSLVKERLSARAHARAARDYDTADAIR
                     DELAAAGIALEDTAAGARWSLARGRSE"
     gene            11150..12106
                     /gene="rlmB"
                     /locus_tag="PL014_06560"
     CDS             11150..12106
                     /gene="rlmB"
                     /locus_tag="PL014_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOT56789.1"
                     /translation="MAGNSQRRGAMRSSSKKGPQVGSGGQRRRGLEGKGPTPKAADRP
                     NHKAHKMAKAADRRSGATGGRTSTRKNKASSEMVAGRNSVLEALRAEVPVTTMYVAGR
                     IDSDDRVREALKLATERSIPVLETPRGELDRLTDGAVHQGLALQVPPYEYAHPSDLID
                     PEAPGIPLVVALDGITDPRNLGAIVRSVAAFGGHGVVVPSRRSVGMTASAWKTSAGAA
                     ARIPVAQATNLTRALEDYRKAGFFVIGLDMDGDVELPDMELASEPIVIVVGSEGKGLS
                     RLVRETCDQIVSVPMSSAVESLNAGIATGVTLYEVARRRRRA"
BASE COUNT         1783 a         4257 c         4415 g         1840 t
ORIGIN      
        1 ccaggtgcgg ttgagccgtg ccagggcgtc ggcggtgacc ctgctggcgc ggtcgtcgtc
       61 gaggagggtc aggtgcgcga cggaggagag ttcgggccag cgggcggaca cgaactcggc
      121 gaagtcaccg tcgatgctca gaggcgtggc aggactcggt cggtgtgctc gccggcctcg
      181 aagatcttcg acgccggtcc cacgatgagg gggtcgggct ggccgacgat gctcgcatcc
      241 ttctgcgcgt agtcgacacg gttgagcagc acccggaagg cctcgaggcg ggcccgcttc
      301 ttgtcgttgc tcttgatgac cgtccacggg gcatcaccgg tgtcggtgta gaagaacatc
      361 gcctccttcg cctcggtgta cgcgtcccac ttgtcgcggc tcgcgaggtc ggtgggggag
      421 agcttccact ggcgcaccgg gtcgttctgg cgggaggcga accgggcggc ctgctcctca
      481 cggctgacgg agaaccacag cttgaccagc gtgatcccgg agttgacgag catgcgctcg
      541 aggtcgggca cctcacgcat gaactcgagg tactgggtgg gggtgcagta gcccatgacg
      601 cgctcgaccc cggcccggtt gtaccaggag cggtccatga acacgagctc gccgccgctc
      661 ggcaggtgct ggacgtagcg ctggaagtac cactgggtcc gctcgatctc ggtgggcttc
      721 tcgagggcga ccacccgtgc accgcggggg ttcaggtgct ccatgaaccg cttgatcgcg
      781 ccgcccttgc ccgcggcgtc gcgaccctcg cagatgacga ggaccttggc gccggtctcc
      841 ttggcgtgca gctggagctt gagcatctcg atctggagct tgcgcttggt gcgctcgtac
      901 tcgtcgcggg gcagcttctt gtcgtacggg tacccctgct gccaggtctt gccgaccttg
      961 acgtcgatct cgacgccgtc ggcctcctcg agatcgtgaa gctcctcgat ctcctcggcg
     1021 tagtcctcgg tcaccgacag tcgcgtgtca tcgttcacga ccttgagact agccaagtgg
     1081 cgcggtgtcc gcgcggcggt tcggtgtgcg ctcaggcacc cacggtggcg ccgtcctcgg
     1141 cgtcctcgtg actgccctcg ctggcggggt cgaactcgcc cgtgaccagc cagatgaccc
     1201 ggtggccgac gctcaccgcg tggtcggcga accgctcgta gtagcgaccc accagggtga
     1261 tgtcgatcgc cgcctccgtc ccgtgtcgcc agctcgggtc ggtgacccgg gcgaacagct
     1321 cgcggtgcag gtcgtccatg gcgtcgtcgt cccgctcgat ctgctgggcc gcctcgaggt
     1381 cgcgcgcagc gatgaccgat ccggccttct cgacgatgcg ttcggcgacg tgacccatct
     1441 gcaggatggt cgagcgcagc tcggcgggca cggcggatgc cgggtagcgc agtcgggcga
     1501 ccttggccac gtgccgcgcg aggtcaccca tccgttcgag gtcgctgctc atccgcatgc
     1561 tcgtgacgat gacccgcagg tcggtcgcga ccggctgctg gcgggcgagc aggtcgaagg
     1621 agagcaggtc gaggtcggca cgcagggcat cgatctcggc gtcagcggcg atgacggagt
     1681 cggcgaggtg gatgtcggcg tcgagcagcg cggtcgtcgc gcggttcatc gcggaggagg
     1741 cgaggtgcgt catctcgacg agcgtctcgg cgacccggtc gagctcctcg tggaaggcct
     1801 tgcgcatcag gggtggttcc ttcgggtggc atgggggtga tgggtgacgg ggccgggccg
     1861 gtgggggctg cggctgctgc cctcgaccct gacagccggg cgtgaaccgc aggcgcaccc
     1921 gaggtgaact tctggcgagg ctggagcggc gggaggtgtt cgtggacgaa ccgggtcgca
     1981 cccgggccgt accctgacgt cgtggaccct gtgctcgcgg cgattctcgg cggggcggtg
     2041 gggctcgtca tcggatccgt cgccgtcctg gcgacacggt ggtccgagcg cagcaccgcc
     2101 gcgcccgaac ctgtcgaccc cccgctgccc cgcggggtcg gcgacgtcct gtcggtgctg
     2161 aagtcgatcg cggtggtcct ggacgccagc gacgccgtgg tcaacacctc ggcgtccgcc
     2221 gtcagctacg gcctcgtgcg ccacggtgag ctcgtgcacc acgaactgcg gcacatcgcc
     2281 cggcaggtgc gccgtgacgg cctgatccgc gaagccgagt tcgacctgcc ccgcggtccg
     2341 aacgcccagg ccagcaccgt gatgcgggtg cgggtggccc cgatcgccgt gtcccacgtg
     2401 ctcatcctgg ccgaggacca cacccaggcc cgccgggtcg aggaggtgcg gcgcgacttc
     2461 gtcgccaacg tcagccacga gctgaagacg cccgtcggcg gcatctcgct gctcgccgag
     2521 gccgtgctcg acgcccgtga cgacccggat gccgtcgagc gcttcgccga gcgcatcctc
     2581 gtcgagtcgg ctcgcctgac tcgactcgtc aaggagatcg tcgacctctc gcggctccag
     2641 gtcgccgaca ccctgcacga gcccgtcatc gtcgacgttc gcgccgtcgc cgccgaggcg
     2701 gtggaccggg tccgcgtcgc cgccgaggct cacggcatcc agctcacgct cgtcggcgag
     2761 gagggcctgc gcgtgttcgg tgatgccgag ctgctcacca ccgcgatcgc gaacctcgtg
     2821 accaacgccg tcaactacag cggctcaggc acccacgtgg gcatcggtgc acggcgcaac
     2881 ggcgacagca tcgaggtgag cgtcaccgac cagggcgagg gcatcccggc cgccgagcag
     2941 gagcggatct tcgaacggtt ctaccgcgtg gatgccgcgc gctcgcgggc gaccggcggc
     3001 accggcctcg gcctggccat cgtcaagcac gtgtgcgcca accacggtgg gcgggtgagc
     3061 gtctggagcc aggaggggcg cgggtccacc ttcacgatga cacttcccgc cgcggccgac
     3121 cggccgcgag tgggcaccga gcagccgcac ccgcagaccc tcgggtcacc cgaggtgcgc
     3181 gaccacgccg acccgtcgcg gacggcatcc gatgtggaga aggaaagggc cctgcatgac
     3241 gcgcatcctg atcgttgagg acgaggtgtc cttctcggac ccgctgtcgt acctgctgag
     3301 caaggagggg tacgacgtca cggtggccga gaacgggcac gacgcgctga ccgccttcga
     3361 cggcgcgggc gccgacctcg tgctgctcga cctcatgctg cccgggttgt ccggcgtcga
     3421 cgtctgccgt gccctgcgcc aacgctccaa cgtgccggtc atcatgctga cggccaagga
     3481 cagcgagatc gacaaggtcg tcgggctcga gctgggggct gacgactacg tcaccaagcc
     3541 gtactcctcg cgcgagctgc tcgcccggat caaggccgtg ctgcggcgac tggccgagcc
     3601 cgaggagctg ctgccgacga ccctggaggc cgggccggtg cgcatggacg tcgagcggca
     3661 cgtcgtgacg gtgcgcgggg gccagctgcc ctttccgctc aaggagttcg agctgctcga
     3721 gatgctgctg cgcaacgcgg gacgcgtgct gacgcgcggc cagctcatcg accgggtgtg
     3781 gggcagtgac tacgtcggcg acaccaagac gctggacgtg cacatcaagc ggttgcgcgc
     3841 caaggtcgag gcggacccgg cgaacccggt gcacatcctc accgtgcgcg ggctcggcta
     3901 caagttcgag gtctgacctg agtcagagcg ccggggcctg ctccttgcgt ccggcagctt
     3961 ggctcccccg acgaccaaag tgccggacgt agccgccggc tcagccgaag acggtgccgt
     4021 tgtggtgcac gcccgccagc accggcacgg tgacgacgtt ctggcccgcc tggggcgtgg
     4081 acaccgtgag ggtgatggtg tcacccgcgc ttgccggcag gccctcgaac tcgatgagcg
     4141 caccggtgtc ggacagcgag ttgccggaga acgcgggcac ggcggccgtc tcggtggacg
     4201 ttgcgccctc gacgccgaag gtcacgttga cggctgagct acccctgttg accgcctgac
     4261 cgatgaggac cgccgggccg tcactgttct cgacgacgag cgcgaggttg cgcagctcaa
     4321 ggccggggat cgacagcgcg acaccgtcgg aggccgggta gtcgtagtcg gtctgggtgg
     4381 gtgagaaggt tgcgcacccg gaggcgagga ggctggccgc cagcagggcg gtggcacgca
     4441 gggcgagggc ggctcgtgtg gtcacgcggc aaggatatcc atgagtgggt gcacggcgtc
     4501 caacggcagg cggcaccgtc cccactgcgg gcgcgtgggg cgcgcgcgat gcatggaaag
     4561 cgctgttctg tcaagacccg attcatgccc tgtggagtgg tctccccgcc cctgacctgc
     4621 gcaaatgggt tgtcgttcca tttctgtggc ctgattctgc gtggtactct gagtgtcgcg
     4681 aaaggggcca acaacacatg gttttcaagg tcggcgaaac ggtcgtgtac ccccaccacg
     4741 gggcagcgcg aatcgaagag atcaagactc gtacgatccg cggagaggag aagctgtacc
     4801 tcaagttgaa ggtcgcccag ggcgatctcg tcatcgaggt accagctgag aactgcgacc
     4861 tcgtcggcgt ccgcgacgtg gtctccaagg aagggctcga caaggtcttc gaggtgctgc
     4921 gcaccccgtt cgccgaggag ccgaccaact ggtcgcgccg cttcaaggcc aacctggaga
     4981 agctcgcctc cggcgacgtc atcaaggtcg ccgaggtcgt tcgtgacctg tggcgccgcg
     5041 acaaggaccg cggcctgtcc gccggtgaga agcgcatgct cgccaaggct cgccagatcc
     5101 tcgtgtccga gctggctctg gcggagaaga ccgacgagga aaaggccgag gtcatcctcg
     5161 acgaggtcct cgcgtcctga caccgggctc acgcccactc cacactgacg tgaccgacgc
     5221 cgacgctgcc ccgagcagcg tcggcgtcgt cgtcgtcgca gccggttccg gtagccgcct
     5281 gggtgctgac gtccccaagg cgttcgtcca cctcgggggc cggcccctgc tcgcccacgc
     5341 gctcgacacc atcgcccgga tgccggggcg caccgaggtc gtcgtcgtcg ccccggcggg
     5401 cctcaccgac ccggatgacc cgctgtggtc gggcgtcgac ctccctgccg gcgcggtggt
     5461 cgtgcccgga ggggccgagc gcaccgactc ggtcgcggcc gggatggccg ccctgccccc
     5521 tggttgcgag atcgtgctcg tgcacgacgc cgcgcgctgc ctgatgccgc tcgccgtgtt
     5581 cctgcgggtg gtcgacgcgg tgcgtgccgg tgcggctggt gcggtccccg gcctgccggt
     5641 ggttgacacc atcaagacgg tcgacgctgc cggggtgatc acggggacgc ccgagcggtc
     5701 agcgctgcgc gcggtgcaga ccccgcaggg gtttcgacga gaggtgctag agcgcgccca
     5761 cgccagcggt cttcaggcca ccgacgacgc ggcgctggtc gagctctgcg gtgacgacgt
     5821 ggtcgtcgtc gacggcgacc cgctgggtgc caaggtgacg accgccgacg acctcgagcg
     5881 ggcgcagtgg ctgctcaggc gggctgccac actgggcgga tgatgcagct gccacgggtc
     5941 ggcgtcgggg tcgacgttca cgcactcgcg cccgagggct ccgatcgcga gctgtgggtg
     6001 gcaggggtgt actggccggg ggagcgcggg ctcgacggcc actccgacgc cgacgtcgca
     6061 gcacacgcgg cctgcgacgc gctcttcacg gccgccggca tcggtgacct gggggcgcac
     6121 ttcggtaccg gtcggccgga gttcgccggg gcctccggcg tgaccctgct ctccgaggcg
     6181 gcccggttgg tgcgtgctgc gggcttcgag gtcggcaaca tcgcggtcca ggtgatcggc
     6241 aaccggccca aggtgggcac gcgccgggcc gaggcggagc gggccctgtc ggatgccgtt
     6301 gacgctcccg tgtcggtgag cggcaccacg accgacgggc tgggcctcac cgggcggggc
     6361 gagggcatcg cggcgatcgc caccgccctc gtcgtggctg ccagctgaac ccgcatccct
     6421 gtcccgcggg cacatccgca gtggggggag gccgctcacg tcagccaccc ccagaggtcc
     6481 gaccgtcagt ggccgcggtc gagccacacc tgcaggtgcg gggcctcgtc gccgatcgtc
     6541 gtcgtgtctc cgtggccggt gagcacgacg gtgtcggccg gcagggtcaa caggcgctca
     6601 cggatcgagt cgatgatcgt cgggaagtcg ctgaacgagc gccccgtcgc accgggcccg
     6661 ccgctgaaca gggtgtcccc gctgagcagc acgccgagcg ccggcgcgtg caggcagcac
     6721 gcaccgggcg cgtggcccgg ggtgtgcacg acggtcagct cgacgtcgcc cacggtgaag
     6781 acctggccgt cggtgagctc gccaccgggc ggctcggtct gcgactggtc ccacagcacc
     6841 cggtcgtccg ggtgcaggga tgccgtggca ccggtcgcgc gcacgaggtc accgaccgcg
     6901 ttgacgtggt cgttgtggcc gtgggtgcag acgatcgcga cgaccctgcg cccaccgacg
     6961 gcctcgagga tggcgggtgc gtcgtgcgcg gcgtcgacga ccagcacgtc gctgtcgtca
     7021 ccgaccagcc acacgttgtt gtcgacctcc cacgagcccc cgtcgagctc gaacaccccg
     7081 gaggtgacca cccgctcgac ccgcacgcca cccggcgacg gtgcgagcgg catccggctg
     7141 tgcgagctca ctggccctca cccatgacga cgacggagcg cagcacctcg ccacggtgca
     7201 tcttgtcgaa ggcggcctcg acgtcggtga tgccgatgcg ctcggtgacg aaggcgtcga
     7261 ggtcgaaccg gccttggcgg tagagctcga cgagcatcgg gaagtcgcgc gagggcaggc
     7321 agtcgccgta ccagctcgac ttcagcgagc cgccgcgacc gaagatctcg atcatcggca
     7381 gggtgacctg cttgtcgggg gtcggcacgc cgacgagcac caccgtgccc gcgaggtcgc
     7441 gggcgtagaa ggcctgctca taggtctcgg gacggccgac ggcctcgacg acgacgtcgg
     7501 cgccgtgccc gcccgtgagc gcgcggatcg cctcgacggc atcctcggtg ctcgagtcga
     7561 cggtgtgggt ggcgccgaag gccttggctg cctcgagctt gcgggcgtcg acgtcgaccg
     7621 cgatgatcgt cgtcgctccg gcgaccttgg ccccggcgat ggccgcgttg ccgaccccgc
     7681 cgcagccgat gacggcgatc gagtcgccgc ggccgacgcc gccggtgttg acggccgcgc
     7741 cgaagccggc catgaccccg cagccgagca ggccgaccgc agcggcatcc gcccggtcga
     7801 ccttggtgca ctgccctgcg gcgacgaggg tcttgtcgat gaaggcgccg atgcccaggg
     7861 ccgggctcag ctcggtgccg tcggtcaggg tcatcttctg cttcgcgttg tgggtggcga
     7921 agcagtacca gggcttgccc ttgaggcagg cgcggcactg gccgcagacg gcccgccagt
     7981 tgaggatcac gaagtcgccc ggggccacct cggtgacgcc ctcgccgacc gactcgaccg
     8041 tgccggcggc ctcgtgcccg agcaggaagg ggtagtcgtc gttgatgcca ccctcgcggt
     8101 agtgcaggtc ggtgtggcag accccgcacg actcgacctt gacgacggct tcacccggcc
     8161 cggggtcggg aacgacgatg tcgacgagct cgacctcggc tcccttggat cgggcgatga
     8221 ctcccttgac ggtctgtggc acgggtactc ctcgggtcgg cgacggtgct gacgagccct
     8281 tcgcgggcgc tggcgtcacg ctagccgccg ctctcggatg ccggtaggcc gggtcggtgg
     8341 ggtcaacttc tccgccctcc ctgtgatgac ggagaagtgt gttcgcggcg gagacatatg
     8401 tccgtcagcg tgcgacttcc ccgccgtcac cgtgatgacg gagaagctca ggtcagctcg
     8461 acccggcgat tgctggcacg ggccagcgcc gatcgcacca gggcgtccac acggcgaggg
     8521 tggtcgaggt cggcccaggt caaccggacc acctcgtagc ccagagcccg gagggcatcc
     8581 tcgcgacgct tctcggcagc caatgcctcc cggccgtcct tgccggcgta cttcacgagc
     8641 ccgtcgaact ccacgatcac gcgagagccc acgaggaagt cggcgcgggc catgaccgtt
     8701 ccggcggcgt cggtgactcg cacctggctg cgcggggcat ggcccaggtc gatcagcagc
     8761 agccgggtcc gggtctcgcc gaccgattcg gccaaggggt cgcacgcgcg cagccatcgt
     8821 tgcgcccgca gtccccggcg cgcagtaggt gccaggtgcc cgatcatctc gaccacgtcg
     8881 tcgatctgga ctcgaccggt cgccagggcc cggtccgcgg cgaccatcac tggcagacgg
     8941 ccctctcgga gcgcaacctg cgcgagggcg acggcaatcg tgacgctgcg gcatccgtcg
     9001 acatccacca cgggcagtga cgggtcggcc gcatgcacgc gaagggctcc ctcacggcgc
     9061 accctgcgca ccagtccgaa caggtcgacg acgtcatgcg gcacgccgaa cagtgggagc
     9121 ccatgcacgg cgagggcgct ctggtgggtg gcggcctcgc ccggcgcgtt gccgcgggtg
     9181 cgcagaacgg ccctggtcat cagagcgagt tgttgctcgg gtgtggcgcc ggcccagtcg
     9241 gaggcgacga cgaaagagcc gcgtcttact cggcgcagca ccccggcgcg caggagggca
     9301 gcgagggcgt gggggtcgac gtcggcgtgg tggacgtcct ggctcgcgat gatgccgttg
     9361 ttggcggctg ccagggcgag gagtcggtcg gacatccctc gatcctgccc gcccggcggg
     9421 gatccacgga gttgtccaca gagcgaagtg agaagtttcc gacccgatgc gggcggcatc
     9481 cgaggtgggg gaggtcggga acgtctccgc cgtcactggg acggcgcaga tcatgggcca
     9541 gggcggagat ataccttcgc ctcggcgcca cgagaccgcc gtcacagggg tggcggagaa
     9601 gaggcgcggc agcctgacag gggtggccga gcaggggcat cgccacctga tccggatgcc
     9661 ggtcgcgggc ttcgcggaat cggtgcaccc gccgcgccgg tagccttacc gggtgactct
     9721 caggcttttc gactccctca cgcgcgagct gcgtgacttc gtccccgtcg tgcccggcaa
     9781 ggcggggatc tacatctgtg ggctcacgac ccaggcacca ccccacctcg gccacgtgcg
     9841 cttcgccgtg gcgttcgacg tgctgcgccg ctggctggcc cgtgggcacg gctacgacgt
     9901 cacgctcatc cgcaacgtca ccgacatcga cgacaagatc ctcgcgaagt cgctggagtc
     9961 cggcgaggcc tggtacgcgg tgtcctaccg caacgagctc gagacctctg ctgcgctcgc
    10021 gtcgctcgga gtgctgcccc cgacctacga gccgcgcgcg accgggcacg tgcccgagat
    10081 ggtcgagctg atggcggccc tcgtcgagcg gggtcacgcc tacgcagccg ccgacggcag
    10141 cggcgacgtg tacttcgacg tgcgcagctg ggccgactac ggcgcgctga cccaccagaa
    10201 ggtcgacgac atggtcgacg cggaggatgc cgacccgcgc ggcaagcgcg acccccgtga
    10261 cttcgcgctg tggaagggtc acaaggccga cgagcccccg accgcgtcgt ggccgacccc
    10321 gttcggcccg ggccgcccgg gctggcacct cgagtgctcg gcgatggccc gcaagtacct
    10381 cggtgacacc ttcgacatcc acggtggtgg cgtcgacctg cggttcccgc accacgagaa
    10441 cgagctcgcc cagtcgcgcg cggccggtct cggcttcgcc aactactggc tgcacaacgc
    10501 ctgggtgacg ctgcacggcg agaagatgag caagtcgctc ggcaactcgt tgcgggtcag
    10561 cgaggtcgtc aaggagcacc gcccgctcgc ggtccgctac tacctcacgg ctgcccacta
    10621 ccgctcgacg atcgagttcc acgccgggtc gctcgaggag tccgacacgg cggtccagcg
    10681 gatcgagggg ttcctgcgtc gtgcgctgcc cggcatcctc acggccctgc cgacgggcga
    10741 cgagacgctg cccgaggact tccgcgaggc catggacgac gacctcggcg tctccagtgc
    10801 cctcgccgcg gtgcacgagg ccgtcaccac cggcaacacc gcgctcgacg acggtgacca
    10861 cgaggatgcc gctgccgtcg cccgcgtcgt catcgccatg accgacgtgc tcggcgtcaa
    10921 cccgctcgac cccgcgtggt ccgccggtga ctcctcggcg gcggatgccc tcgcgagcct
    10981 tgactcactc gtcaaggaac gcctctcggc ccgggcccac gcccgtgccg ctcgtgacta
    11041 cgacaccgcg gatgccatcc gcgacgagct cgccgccgca ggcatagcgc tcgaggacac
    11101 cgccgccggg gcccgctggt ccttggcgcg gggacggagc gaatgactca tggccggtaa
    11161 ctcccagcgc cgcggcgcga tgcgcagttc gagcaagaag ggcccgcagg tcggctccgg
    11221 aggtcagcga cgtcgtgggc tcgagggcaa gggcccgacg cccaaggcgg ccgaccgccc
    11281 gaaccacaag gcacacaaga tggccaaggc ggcggaccgc cgcagtggcg caacgggtgg
    11341 gcgcacgagc acgcgcaaga acaaggcgag cagcgagatg gtcgccgggc gcaactcggt
    11401 gctcgaggcc ctgcgcgccg aggtgcccgt cacgacgatg tacgtcgccg gacggatcga
    11461 ctccgacgac cgggtccgtg aggcgctcaa gctcgccacc gagcgctcca tccccgtgct
    11521 cgagacccct cgcggagaac tcgaccgcct caccgacgga gcggtgcacc agggcctggc
    11581 ccttcaggtg ccgccctacg agtacgccca ccccagcgac ctcatcgatc ccgaggcccc
    11641 cggcatcccg ctcgtcgtcg cgctcgacgg catcaccgac ccgcgcaacc tcggtgcgat
    11701 cgtgcgttcg gtcgcggcct tcggtgggca cggtgtcgtc gtgcccagcc ggcgctcggt
    11761 gggcatgacg gcctccgcgt ggaagacctc cgccggagcg gcagcccgca tcccggtcgc
    11821 ccaggccacc aacctgaccc gggctctcga ggactaccgc aaggccggct tcttcgtcat
    11881 cggcctcgac atggacggcg acgtcgagct gcccgacatg gagctcgcgt cggagccgat
    11941 cgtcatcgtc gtgggctccg agggcaaggg tctgtcccgg ctcgtgcgcg agacctgcga
    12001 ccagatcgtg tccgttccga tgtcctcggc ggtcgagtcc ctcaacgccg ggatcgccac
    12061 cggggtgacc ctctacgagg ttgcccgcag gcgccgacgg gcctgacctc tggcgtcgtc
    12121 gcggccggat gccggtgggc ggcgaccggg ttcagcgccc gggaacgcgg cggtttgggt
    12181 cgagatgacc ccgtggctgt ggaaggatcg gcgccgccgg aggactccgg cacctggggg
    12241 gtgcgcaacc ccacactcgt atcgagaggg aactcatgtc tgactacgga tcgac
//
LOCUS       DAOSOC010000064        12196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5197_length_12196_cov_9.497982, whole genome shotgun sequence.
ACCESSION   DAOSOC010000064 DAOSOC010000000
VERSION     DAOSOC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5197_length_12196_cov_9.497982"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(397..470)
                     /locus_tag="PL014_06565"
     tRNA            complement(397..470)
                     /locus_tag="PL014_06565"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Phe,seq:gaa)
     gene            complement(496..569)
                     /locus_tag="PL014_06570"
     tRNA            complement(496..569)
                     /locus_tag="PL014_06570"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(533..535),aa:Asp,seq:gtc)
     gene            complement(595..667)
                     /locus_tag="PL014_06575"
     tRNA            complement(595..667)
                     /locus_tag="PL014_06575"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(631..633),aa:Glu,seq:ttc)
     gene            complement(791..1168)
                     /locus_tag="PL014_06580"
     CDS             complement(791..1168)
                     /locus_tag="PL014_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT56790.1"
                     /translation="MGGLVRVDPASGEAPFWQVRRQVSEAIADGRLPVGTKLPPIRAL
                     ADDLGLAANTVARAYKELEAAGLVLTRGRLGTVVTSDAVDGLGADAAVRLAAGDYARA
                     ARAAGIPDEDAVALVREALTRLP"
     gene            complement(1201..2250)
                     /locus_tag="PL014_06585"
     CDS             complement(1201..2250)
                     /locus_tag="PL014_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT56791.1"
                     /translation="MVALLEPLEAPVHPVEALRRVAFLLERTRAGTYRVEAFRNAVKT
                     LKTVDAGELAERAAAGTLQEIPGIGKSTSGVIAQALAGELPAYLATLEAKAGGPLVEG
                     GEALYAALKGDCHLHSDWSDGGSSIDEMVLTGIELGHEWLVLTDHSPQLRVANGLSAE
                     RLTQQIGIVEAIDRSLGGAFRLLKGIEVDILDDGGLDQSDAMLARLDLVTASVHSKLR
                     MNAEPMTARMVAAVSNPRVNVLGHCTGRLVEGSRGTRPQSDFDARAVFEACAEHGTAV
                     EINSRPERCDPPDDLLLLALELGCLFAIDSDAHAPGQIDMKAYGCERAERLGVPAERI
                     VTTWDADRLLTWANP"
     gene            complement(2269..3486)
                     /locus_tag="PL014_06590"
     CDS             complement(2269..3486)
                     /locus_tag="PL014_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HOT56792.1"
                     /translation="MPFDVASVRAHFPSLAEGAAHFDGPGGSQVPDVVGRAIADTVTA
                     AISNRGTFTPSERRADAVVADFRSAYADFLGADPRGIVHGRSATALTYDFSRTLAKNW
                     GPGDEIVVTRLDHDSNVRPWVQAAQSAGATVRWVDFDPATGELTADAVAAVVTERTRL
                     VATTAASNLIGTKPPVCHISRLAHEVGALHWVDGVHATAHASVDIVALDADVWTCSPY
                     KFMGPHCGVLAASPDLLETLHPDKLLPSTNDVPERFEFGTLPYEALAGATAAVDFIAG
                     LAESSEGDDTASGTRRERIEASMKAVHAHERALLDVLEPALRGLPGVTVHSNAAKRTP
                     TLLFTVEGRDPQEIRGLLAERGVNAPAGHFYAIECSRHLGLGDVGGVRVGLAPYTSEN
                     DIDRLVTGLREIL"
     gene            complement(3563..4999)
                     /locus_tag="PL014_06595"
     CDS             complement(3563..4999)
                     /locus_tag="PL014_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HOT56793.1"
                     /translation="MRFLNDLQPQHDLTYDDVFMVPNRSSVTSRLDVDLSSADGTGTT
                     IPLVVANMTAIAGRRMAETTARRGALTVIPQDIPAEIVRDVIAWQKSRHLVYDTPITL
                     TPGTTVGEALVLLHKRAHGAALVTDGDRPVGIVTETDLTGVDRFTQLEDVMVRDMVSL
                     PDGVDVESAFDTLSHARRRLAPVVTPDGRLVGILTRPGALRAHLYSPATDDTGRLRIA
                     AAVGINGDVAAKAADLLDAGADTLVIDTAHGHQEKMIEALRAVRALDPQVPVVAGNVV
                     SAQGTRDLIEAGADIVKVGVGPGAMCTTRMMTAVGRPQFSAVLECAAAARELGGHVWA
                     DGGVRHPRDVALALAAGASNVMIGSWFAGTLESPGDLYTDEAGRQYKESFGMASSRAV
                     RHRTASDTAYERARKAMFEEGISSARMYVDPARPSVEDVIDEIVAGLRSSCTYAGART
                     LEEFHDRATVGIQSTAGFAEGRPLHTSW"
     gene            5244..>5962
                     /locus_tag="PL014_06600"
     CDS             5244..>5962
                     /locus_tag="PL014_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HOT56794.1"
                     /translation="MAASPADAARRTGLRRMRLVALGLLAFAAAVFLATLRLDHDGVW
                     GYVNTAAEAAMVGALADWFAVTALFRHPLGLPIPHTAIIPKRKNEIGRNLQEFVTENF
                     LTEEIARERLATARVGERVGQWLGMPANRRRVMVEAVRVGRAGLGRLSDDEVRALVQD
                     FLVPRLVREPVSPIAGALLEGVVEQQTHQGLVDLALEHLREWLADNPGTYAAVLGERA
                     PWWSPPWVDDKVIGWTYQQVIA"
     assembly_gap    5963..6062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6063..6646
                     /locus_tag="PL014_06605"
     CDS             <6063..6646
                     /locus_tag="PL014_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HOT56795.1"
                     /translation="PPWVDDKVIGWTYQQVIAWLRDIENDPRHPARQAFDDLLRRLAV
                     DLQQDPEVMARAEALKVLLLTHPQVPETALGLWQSFRTALTTAMDDETSYFHTRGDEL
                     LENLGNHLVHDEVWRARLEARLGEAVSFFVNTYGSELAEVISVTVDRWDGKEAAERIE
                     LHVGRDLQFIRINGTIVGALAGLVIHGVAQAVG"
     gene            complement(6836..7987)
                     /locus_tag="PL014_06610"
     CDS             complement(6836..7987)
                     /locus_tag="PL014_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT56796.1"
                     /translation="MRIGELASKVGVSTHVLRAWESRYGLLRPQRSAGGYRLYGHEDE
                     RRVREVIALRDQGVSAAEASRRVLEADRSGLSAIDDDIDDDATTASGSSFAAGHRRAG
                     GRAGAVGTASGMGVDTSVPLRSDPPALVAQLLDAVSTLDEDRAHAVLDVAFGERSVDS
                     AIIDVLLPLFVRVGELWELGRIGIAQEHFASSLVRRRLGAMSLTWGVGNGPVAVLACP
                     PGEFHDIVLLSFGVLLGRTGWRIRYLGPDTPVHSLAAAARLTQADAVVLACRRPSGFR
                     AHSTALRRLGEDYPVWLAGRGATPRVLEEVGVRHLGADLVGAVAELTATARDRRNQRE
                     ATARPDGDGIPVDDSRANGNGIQGDGIRTDGIQGDGIRTDATTPTSATA"
     gene            8111..8584
                     /locus_tag="PL014_06615"
     CDS             8111..8584
                     /locus_tag="PL014_06615"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT56797.1"
                     /translation="MLPQVLRRHEAAAMTTTIERRVTTTTDPDRVFAYLTDFRNAKQW
                     DSGTTSCELVSGDGGPGTVYRNVSEFAGTTVELEYTTQTVEQPRFVIVGRNDTTVSHD
                     TITVTPHGPNGSSVHYRAEFTFTGLARFAAPLMKPLLDRLGDRTAEQLKSTLDRL"
     gene            8581..9609
                     /locus_tag="PL014_06620"
     CDS             8581..9609
                     /locus_tag="PL014_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOT56798.1"
                     /translation="MTDSPTTSRRALVTGATGYIGSRLVPRLLDEGWQVRVLTRSADK
                     VAGRAWRDRVDVVEGDAASPDTLARALADVDVAYYFLHSMDGQGNFVERDRELAQTFG
                     RCAADAGVGRIVYLSGLHPHDGELSDHLASRVEVGDILLASGVPTAVLQAAVILGSGS
                     ASFEMLRHLTTRLPAMITPKWLDNRIQPIAVRDVLHLLVAAADLPPEQNRTFDIGGPD
                     VLTYREMINRFARIAGLRPRLIVSVPVLTPYLASQWVGLVTPVPTGVAKPLVGSLLHE
                     VVCTERDIDDLVGSPPQGYLDYDRAVADALAGDGVDPRPEPGTADPARITAADPDWAG
                     GRRPRGQS"
     gene            9606..10328
                     /locus_tag="PL014_06625"
     CDS             9606..10328
                     /locus_tag="PL014_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOT56799.1"
                     /translation="MNVVDEFRAFLRAALVTPVERDHTESDAAFRRRRVVAAITLVIG
                     GVILAIALRIEPGDPRFYGATFALAGVWAAGAFLSGPLHLGRGHTRGGSGSSRAVVQS
                     LALGVMLLALFMVGSLVVARIPVLREPVQELLDHARVGSLPLVALITVVNGIAEELYF
                     RGALYAAVGRRHAVAVTTVVYALVTAAAGIPLLVLAAALVGLVVAFQRRVTGGILGPI
                     VTHLTWSLGMLFLLPPVLDALS"
     gene            10339..11712
                     /locus_tag="PL014_06630"
     CDS             10339..11712
                     /locus_tag="PL014_06630"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOT56800.1"
                     /translation="MTAILWLRRDLRRADHPALLAAREAATASGGDLVVAFVVDPRLW
                     DGGGAARRAWLAATLKATDEAYDGALTLLHGNPRTVIPELASRVGATSVHVSRETTPA
                     GRRRDEVVAGALRSAGVEWVETGTPYAVGPGLVVNGSGDPYKVFTPFSKAWRAHGWPA
                     PAITPRELPLRRVDSDPRATAEVTAALEAPGLPELPPAGEKAALARWREWRERALTAY
                     ADDRNRPDLDGTSRLSPYLKLGVVHPRTLLAELAGESGKGAHTYESELAWREFYADVL
                     WHHPRTAWEDLRPEMSAMRYDEPQDAIEAWKAGRTGYPIVDAGMRQLLAEGWMHNRVR
                     MIAGSFLTKDLHVWWPLGARHFMEHLIDGDIASNNHGWQWVAGTGTDASPYFRVFNPV
                     TQGLRFDPDGDYVRRWVPELAHVPGAAAHEPWDVADGYDHGYPARIVDHAAERIEALD
                     RLQATKS"
     gene            11979..>12196
                     /locus_tag="PL014_06635"
     CDS             11979..>12196
                     /locus_tag="PL014_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOT56801.1"
                     /translation="MFTSLPSSLLAAGAPAAAEPATAIQSIGTPTLWALTIGAVIVLV
                     IVDFLITRRPHEVSMKEALGWSAFYIAIP"
BASE COUNT         1694 a         4305 c         4335 g         1762 t
ORIGIN      
        1 ctagaaccca gctgggccgg atgtgactgg tggcggcgga tgccggtcct gacgatcccc
       61 gttggcagtg ggcatcgatt tcagctgagg ctgacaaacg gtccactatc tgaccggccg
      121 aacgtccggc agtgggccga acgtgacctt tggtcgaggc gtggggtgtg atctgggcat
      181 gaggtggcgg ccccggcggc gcgccagtcg gttcaggccg gggagcggtc gacgagccct
      241 gcgacagcca agccgcctca ccaccgctgg agagggcgcg atcaccaagc ccactaccgc
      301 gtctgaaagt cgtggccctc gagcctcgtg ttagcaagcg ttagcagact tcagattcga
      361 ggaagttctc acaagcctgt gacctgcggt tttgtgtggc caggggcggg gtcgaaccgc
      421 cgaccttccg attttcagtc ggacgctcgt accaactgag ctacctggcc gggtggctcc
      481 ctcgtgagga gccggcgacc ccgacgggac tcgaacccgc gacctccgcc gtgacagggc
      541 ggcgcgctaa ccaactgcgc tacggggcct tgtgtaaggc cttgtgacat tgcgtatccc
      601 caacgggatt cgaacccgtg ctaccgccgt gaaagggcgg ggtcctgggc cgctagacga
      661 tggggacccg acctcagtca gatccacaac gtcacgcaga ccgcagcgtt cggatccgtt
      721 gaggactttg gaagcatagg ggacgaatcc cgcccgcacc aaaacgagtc cgggaacccc
      781 tgccccggtc tcacgggagg cgggtgagcg cctcccgaac cagcgccacg gcatcctcgt
      841 ccgggatgcc agccgcacga gccgcgcggg cgtagtcacc ggccgcgaga cgcaccgcag
      901 cgtccgcgcc gaggccgtcg acggcatccg aggtgacgac ggtgcccagg cgcccgcggg
      961 tgaggacgag cccggccgcc tcgagttcct tgtaggcacg agccacggtg ttcgcggcga
     1021 gcccgaggtc gtcggcgagc gcccggatcg gcggcagctt cgtgccgacg ggcaggcggc
     1081 cgtcggcgat cgcctcggac acctgccggc gcacctgcca gaacggggcc tcgccggatg
     1141 ccgggtcgac ccgcacgagg ccgcccatcg ccgtgcgcac agggccgtgc acggggccgc
     1201 tcacgggttc gcccaggtaa ggaggcggtc ggcatcccac gtcgtgacga tgcgctcggc
     1261 cggcaccccg agccgctcgg cacgctcgca gccgtaggcc ttcatgtcga tctggccggg
     1321 cgcgtgggcg tcggagtcga tcgcgaacag gcagccgagc tccagggcga gcaggagcag
     1381 gtcatcgggt gggtcgcagc gttcgggccg gctgttgatc tcgaccgcgg tgccgtgctc
     1441 ggcgcaggcc tcgaacaccg cacgcgcgtc gaagtcggac tgtgggcggg tgccgcgcga
     1501 gccctcgacg agccgccccg tgcagtggcc gagcacgttc acccgcgggt tcgacacggc
     1561 tgccaccatg cgtgccgtca tgggttctgc gttcatccgc agcttgctgt gcacggatgc
     1621 cgtgacgagg tcgagcctcg cgagcatcgc gtcgctctgg tccaggcccc cgtcgtcgag
     1681 gatgtcgacc tcgatgccct tgagcagccg gaacgcccca ccgagcgagc ggtcgatggc
     1741 ctcgacgatg ccgatctgct gggtgagccg ctcggcgctg agcccgttcg cgacccgcaa
     1801 ctgggggctg tggtcggtga gcacgagcca ctcgtggccg agctcgatgc ccgtcagcac
     1861 catctcgtcg atggaggagc cgccgtcgct ccagtccgag tgcaggtggc agtcgccctt
     1921 cagcgcggcg tacagcgcct caccgccctc gaccagcggc ccgccggcct ttgcctccag
     1981 cgtggccagg taggcgggca gctcaccggc gagggcctgc gcgatcacgc cgctcgtcga
     2041 cttcccgatg ccggggatct cctgcaacgt acccgcggcg gcgcgctcgg cgagctcacc
     2101 ggcatccacg gtcttgagcg tcttcacggc gttgcggaag gcctcgaccc ggtaggtgcc
     2161 cgcccgcgtg cgctcgagca ggaacgcgac gcggcgcagc gcctcgaccg ggtggaccgg
     2221 cgcctcgagc ggttcgagca gagcgaccat cgacagttgc cccgcggctc agaggatctc
     2281 gcgcagcccg gtgacgagcc ggtcgatgtc gttctcgctg gtgtagggcg cgagccccac
     2341 gcgcaccccg ccgacgtcgc ccaggccgag gtggcgcgag cactcgatgg cgtagaagtg
     2401 cccggccggg gcgttcaccc cgcgctccgc gagcagccca cggatctcct gcgggtcgcg
     2461 cccctccacg gtgaagagca gggtcggcgt gcgcttggcc gcgttgctgt gcacggtgac
     2521 tccgggcagg ccgcgcagtg cgggctcgag cacgtcgagc agcgcgcgct cgtgcgcgtg
     2581 caccgccttc atcgaggctt cgatgcgctc gcgacgcgtg ccggatgccg tgtcgtcacc
     2641 ctccgaggac tcggcgagcc ctgcgatgaa gtcgacggcc gcggtggccc cggcgagggc
     2701 ctcgtacggc agggtgccga actcgaaccg ctcggggacg tcgttcgtcg agggcagcag
     2761 cttgtcgggg tgcagcgtct cgagcaggtc gggggaggcg gccagtaccc cgcagtgcgg
     2821 ccccatgaac ttgtacggcg agcacgtcca gacgtcggcg tcgagcgcga cgatgtcgac
     2881 cgaggcgtgc gcggtggcat gcaccccgtc cacccagtgc agcgcgccga cctcgtgcgc
     2941 cagccgggag atgtggcaca ccgggggctt ggtgccgatg aggttcgagg ccgcggtggt
     3001 cgcgacgagg cgcgtgcgct cggtgacgac ggcggccacg gcatccgcgg tcagctcgcc
     3061 ggtggccggg tcgaagtcga cccagcgcac cgtggcgccg gcggactgcg cggcctgcac
     3121 ccacgggcgc acgttcgagt cgtggtcgag gcgggtcacg acgatctcgt cacccgggcc
     3181 ccagttcttg gccagggtgc gcgagaagtc gtaggtgagg gcggtcgcgc tgcggccgtg
     3241 gacgatgccg cgcgggtcgg caccgaggaa gtcggcatag gccgagcgga agtcggcgac
     3301 gacggcatcc gcccgccgct ccgagggggt gaaggtgccg cggttggaga tcgcggcggt
     3361 cacggtgtcc gcgatcgcgc ggccgacgac gtcgggcacc tggctcccac cgggcccgtc
     3421 gaagtgggca gcaccctccg cgagcgaggg gaagtgggca cggaccgagg cgacgtcgaa
     3481 cggcataccc ggcatcctgc ctcaatcccg acgacgtccg gcaggttggt gccccgagca
     3541 accaccgtgc cggacgctca ggtcaccagg aggtgtgcag ggggcggccc tcggcgaacc
     3601 cggcggtgct ctggatgccg accgtcgcgc ggtcgtggaa ctcctccagg gtgcgggctc
     3661 cggcatacgt gcaactgctg cgcaggcccg cgacgatctc gtcgatgacg tcctcgacgc
     3721 tcgggcgggc cgggtcgacg tacatgcggg ccgagctgat gccctcctcg aacatcgcct
     3781 tgcgcgcccg ctcgtaggcc gtgtcgctgg ccgtgcggtg gcgcaccgcc cgcgagctcg
     3841 ccatgccgaa cgactccttg tactggcggc ccgcctcgtc ggtgtagagg tcgccgggcg
     3901 actcgagcgt gccggcgaac cacgagccga tcatgacgtt gctcgcgccg gcggccaggg
     3961 ccaacgccac gtcgcgcgga tgccggacgc ccccgtccgc ccagacgtgg cctcccagct
     4021 cgcgcgccgc agcggcgcac tccagcaccg cggagaactg cgggcggccg acggcggtca
     4081 tcatccgggt cgtgcacatc gcgcccggcc cgaccccgac cttgacgatg tcggcgccgg
     4141 cctcgatgag gtcacgggtg ccctgcgccg agacgacgtt gcccgcgacc acggggacct
     4201 gggggtcgag cgcccgcacc gcacgcaggg cctcgatcat cttctcctgg tggccgtggg
     4261 cggtgtcgat gacgagggtg tcggcaccgg cgtcgagcag gtcggctgcc ttggcggcga
     4321 cgtcaccgtt gatgccgacg gccgcggcga tccgcagcct gccggtgtcg tcggtggcgg
     4381 gggagtacag gtgggcgcgc agggcgcccg ggcgggtgag gatgccgacg agccgaccgt
     4441 cgggggtcac caccggtgca aggcggcgcc gggcgtggga gagggtgtcg aaggccgact
     4501 cgacgtcgac cccgtcgggc agcgacacca tgtcgcgcac catgacgtcc tcgagctggg
     4561 tgaaccggtc gaccccggtg aggtcggtct cggtgacgat gccgaccggg cggtcgccgt
     4621 cggtgaccag ggcggcaccg tgggcgcgct tgtgcagcag cacgagggcc tcgccgacgg
     4681 tcgtgccggg ggtcagcgtg atgggggtgt cgtacacgag gtggcgagac ttctgccacg
     4741 cgatgacgtc gcgcacgatc tcggccggga tgtcctgcgg gatgaccgtg agggccccgc
     4801 ggcgggcggt cgtctcggcc atgcggcgcc cggcgatggc ggtcatgttc gcgacgacga
     4861 gcgggatcgt cgttcccgtg ccgtcggccg aggacaggtc gacgtcgagc cggctcgtca
     4921 ccgacgaccg gttcggcacc atgaagacgt cgtcgtaggt caggtcgtgc tgcggctgga
     4981 ggtcgttgag gaagcgcacc ccacccagcg tacggcgggt ggatgccggg cgcgggtggt
     5041 gcgtggccgt gcgggcggtt agagcggggc gggaggtgag cgacggcatc cgacctcgcg
     5101 tacccccatc gaccaatagg tcactccagg tacgcccgcc cacgaggcac ctccacccac
     5161 atcgaccaat aggtcactgc gggtacagcc cgcccccgca aggctgggcg gacccatgag
     5221 gcaggatcgg gcgcgtgagt ctcatggcag catcccccgc cgatgccgca cgccgcaccg
     5281 ggctgcggcg gatgcggctc gtcgcgctcg gcctgctcgc gttcgcggcg gccgtcttcc
     5341 tcgccacctt gcgcctggac cacgacggcg tgtggggcta cgtcaacacc gcggccgagg
     5401 cggcgatggt gggggcgctc gccgactggt tcgccgtcac ggcgctgttc cggcatccgc
     5461 tcggcctgcc gatcccccac acggcgatca tcccgaagcg caagaacgag atcgggcgca
     5521 acctccagga gttcgtcacc gagaacttcc tcaccgagga gatcgcgcgc gagcgcctcg
     5581 cgacggcgcg cgtgggtgag cgcgtcggtc agtggttggg gatgccggcc aatcgccgcc
     5641 gcgtgatggt cgaggcggtg cgcgtcggcc gtgcggggtt ggggcggctg tcggatgacg
     5701 aggtccgcgc gctggtgcag gacttcctcg tgccgcgact ggtcagggag ccggtgtcgc
     5761 cgatcgccgg cgccctgctc gagggggtcg tcgagcagca gacccaccag gggctggtcg
     5821 acctggccct ggagcacctg cgcgagtggc tggccgacaa ccccggcacg tacgcggcgg
     5881 tgctcggcga gcgcgccccg tggtggtcgc cgccgtgggt cgacgacaag gtcatcggct
     5941 ggacctacca gcaggtcatc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nncgccgccg tgggtcgacg acaaggtcat cggctggacc taccagcagg tcatcgcctg
     6121 gctgcgggac atcgagaacg acccgcgcca ccccgcgcgc caggccttcg acgacctgct
     6181 gcggcgcctg gccgtggacc tccagcagga ccccgaggtc atggcccgtg cggaggcgct
     6241 caaggtgctg ctgctcaccc acccgcaggt gccggagacc gcgctcgggc tttggcagtc
     6301 gttccgcacc gccctgacga cggcgatgga cgacgagacc tcgtacttcc acacccgcgg
     6361 tgacgagctg ctcgagaacc tcggcaacca cctcgtgcac gacgaggtgt ggcgggcccg
     6421 gcttgaggcc cgcctcggcg aggcggtgtc cttcttcgtc aacacctacg gcagcgagct
     6481 ggccgaggtc atctcggtca cggtcgatcg ctgggacggc aaggaggccg ccgagcgcat
     6541 cgaactgcac gtcggccgcg acctgcagtt catccggatc aacggcacga tcgtcggggc
     6601 gctcgccggc ctggtgatcc acggggtggc ccaggcagtc ggctgacccc cctcgagccc
     6661 tcaacgacac gccgccgcgt cccgacatcg accaataggt caatccaggt acgcgcgctg
     6721 actggccggg tgtacctgcg gtgacctatt ggtcgatgtg ggtacgcgaa ctgagtggct
     6781 gggtgtacct gcggtgacct attggtcgat ctgggtacgc cggctcgcgt gcgggtcagg
     6841 cggtggcgga ggtcggggtg gtcgcgtcgg tccggatgcc gtcgccttgg atgccgtcgg
     6901 tccggatgcc gtcgccttgg atgccgttgc cgttggcgcg gctgtcgtcg actgggatgc
     6961 cgtcgccgtc gggtcgggcc gtggcctcac gctggtttcg gcggtcgcgg gcggtggcgg
     7021 tcagctcggc cacggccccg acgaggtcag ccccgaggtg acggactccc acctcctcga
     7081 gcacccgcgg cgtcgcgccg cgaccggcga gccacaccgg gtagtcctcg ccgagccgcc
     7141 gcagcgccgt ggagtgcgcg cggaaacctg acggccgacg acaggccagc acgacggcat
     7201 ccgcctgggt gagccgcgcg gccgccgcca gcgagtgcac cggggtgtcc ggcccgaggt
     7261 agcggatccg ccacccggtg cgcccgagca gcacgccgaa gctcagcagc acgatgtcgt
     7321 ggaactcgcc gggcgggcag gcgagcaccg ccaccggccc gttgcccaca ccccacgtga
     7381 gggacatcgc cccgaggcgg cggcgcacga ggctgctcgc gaagtgctcc tgcgcgatgc
     7441 cgatgcgacc gagctcccag agctcaccga cccgcacgaa caggggcagc aggacgtcga
     7501 tgatcgcgct gtcgacggac cgctccccga aggcgacgtc gagcacggcg tgtgcccggt
     7561 cctcgtcgag ggtggacacc gcgtcgagca gctgcgcgac gagcgccggc ggatcggaac
     7621 gcagtggaac gctcgtatcc acgcccatgc cggatgccgt gcccacggct ccggcgcgcc
     7681 ccccggccct tctgtggccc gcggcgaagg aggagccgct ggcggtggtc gcgtcgtcgt
     7741 cgatgtcgtc gtcgatggcg gacagaccgg accggtcggc ctcgagcact cggcgggagg
     7801 cctcagcggc gctcacgccc tggtcccgca gggcgatgac ctcgcggacg cggcgttcgt
     7861 cctcgtggcc gtagaggcgg tagcccccgg ccgatcgctg cgggcgcaag agcccgtagc
     7921 ggctttccca tgcccgaagg acatgggtgc tgacgcccac tttggaggcg agctcaccaa
     7981 tccgcatcgt cggcggtatc gaactcctca cgagagtcac cgtagacctg agacagagtt
     8041 ttgacaaaca ctcaacacaa cgttcgccaa acatttgacg cgcttctgaa ggtcggcata
     8101 ctgtcgagtc atgctccccc aagtcctgcg ccggcatgag gccgcggcca tgaccacgac
     8161 catcgagcgc agggtgacga cgacgaccga cccagacagg gtgttcgcgt acctgaccga
     8221 cttccgcaac gcgaagcagt gggactccgg cacgacgtcc tgcgagctgg tctcgggtga
     8281 cggtggcccc ggcaccgtct accgcaacgt ctccgagttc gccggcacca ccgtcgagct
     8341 ggagtacacg acgcagaccg tcgagcagcc gaggttcgtc atcgtcgggc gcaacgacac
     8401 gacggtcagc cacgacacca tcaccgtcac gccgcacggg ccgaacgggt cgtcggtgca
     8461 ctaccgcgcc gagttcacct tcaccgggct ggcccggttc gccgcgccgc tgatgaagcc
     8521 gttgctcgac cgcctcggcg accgcaccgc ggagcagctg aagagcacgt tggaccggct
     8581 gtgaccgact ccccgacgac ttcccggcgc gcgctcgtca ccggtgccac cggctacatc
     8641 ggctcgcgcc tcgtcccccg tctgctcgac gaggggtggc aggtgcgggt gctcacccgc
     8701 tccgctgaca aggtcgcggg gcgcgcctgg cgtgaccggg tcgacgtggt ggaaggggat
     8761 gccgcctcgc ccgacacgct cgcacgcgcg ctcgccgacg tcgacgtcgc ctactacttc
     8821 ctgcactcga tggacggcca gggcaacttc gtcgagcgcg accgcgagct cgcgcagacc
     8881 ttcgggcggt gcgcggcgga tgccggggtg ggccggatcg tctacctctc ggggctgcac
     8941 ccccacgacg gtgagctgag cgaccacctc gcgtcccggg tcgaggtcgg cgacatcctg
     9001 ctcgcgagcg gggtgccgac ggcggtgctc caggcggcag tgatcctggg ctcgggctcg
     9061 gcatcgttcg agatgctgcg ccacctgacg acccgtctcc ccgcgatgat cacgccgaag
     9121 tggctcgaca accgcatcca gccgatcgcc gtgcgtgacg tgctgcacct gctcgtcgcg
     9181 gccgcagacc tgccgcccga gcagaaccgc accttcgaca tcggtggccc ggacgtgctg
     9241 acctaccgcg agatgatcaa ccggttcgcc cggatcgccg gtctgcgtcc gcgcctcatc
     9301 gtctcggtac cggtgctcac cccctacctc gcgagccagt gggtggggct cgtgaccccg
     9361 gtgcccacgg gcgtcgcgaa gccgctggtc gggagcctgc tccacgaggt cgtgtgcacc
     9421 gagcgcgaca tcgacgacct cgtcggttcc ccgccgcagg gctacctcga ctacgaccgg
     9481 gccgtcgccg acgccctcgc cggtgacggg gtcgacccgc gccccgagcc cggcaccgcc
     9541 gacccggcgc gcatcaccgc tgccgacccc gactgggccg ggggacgccg cccgcgcggg
     9601 cagtcgtgaa cgtcgtcgac gagttccggg cgttcctgcg tgccgccctg gtgacgcccg
     9661 tggagcgcga ccacaccgag agcgatgccg cgttccgccg acgccgcgtc gtcgccgcca
     9721 tcacgctggt catcggtgga gtcatcctcg cgatcgcgct gcgcatcgag cccggggacc
     9781 cacgcttcta cggggccacc ttcgcgctgg cgggtgtctg ggccgctggg gcgttcctct
     9841 cgggccccct gcacctcggg cgcggacaca cccgaggcgg ctctgggtcc agccgcgccg
     9901 tcgtgcagtc gctcgccctc ggggtgatgc tgctggcgct gttcatggtc gggtccctgg
     9961 tcgtggcccg catcccggtg cttcgcgaac ccgtccagga actgctcgac cacgcccggg
    10021 tcggctcgct gcccctggtc gccctcatca ccgtcgtcaa cggcatcgcc gaggagctct
    10081 acttccgcgg agccctctac gccgccgtcg ggcgccggca cgcggtggcg gtgacgacgg
    10141 tggtctacgc cctcgtgacc gccgcggccg gcatccccct gctcgtgctc gccgcggccc
    10201 tcgtcggtct cgtcgtggcc ttccagcggc gggtgaccgg cggcatcctc ggccccatcg
    10261 tcacgcacct cacctggtcg ctgggcatgc tcttcctcct cccgcccgtg ctcgacgcgc
    10321 tgtcgtgaaa ggccccgcat gaccgcgatc ctctggctgc gccgcgacct gcgccgtgcc
    10381 gaccaccccg ccctgctcgc cgcccgggag gcggccacgg catccggggg cgacctcgtc
    10441 gtcgcgttcg tcgtcgaccc gcgcctgtgg gacgggggag gggccgcgcg gcgcgcctgg
    10501 ctggcggcca ccctgaaggc caccgacgag gcctacgacg gtgccctgac actgctccac
    10561 ggcaaccccc gcaccgtcat ccccgagctc gcatcgcgcg tcggggccac gtcggtgcac
    10621 gtgtcgcgtg agaccacccc ggccgggcga cgccgtgacg aggtcgtggc cggggcgctg
    10681 cgatccgccg gggtcgagtg ggtcgagacg ggcacgccgt atgccgtggg gccgggtctg
    10741 gtcgtcaacg gctcggggga cccgtacaag gtcttcaccc cgttctcgaa ggcgtggcgt
    10801 gcacacgggt ggccggcgcc ggccatcacc ccgcgagagc tccccctgcg gcgagtcgac
    10861 agcgaccccc gggccaccgc tgaggtgacc gctgcgctcg aggctcccgg cctgccggaa
    10921 cttccccctg ccggggagaa ggcggccctc gcgcggtggc gcgagtggcg cgaacgggcg
    10981 ctcacggcat acgccgacga ccgcaaccgg cccgacctcg acggcacctc gcggctctcg
    11041 ccgtacctga aactcggtgt cgtgcacccg cgcacgctgc tggccgagct ggcgggggag
    11101 tcgggcaagg gggcgcacac gtacgagagc gagctcgcgt ggcgtgagtt ctacgccgac
    11161 gtgctctggc accacccgcg caccgcgtgg gaggacctgc gccccgagat gtcggccatg
    11221 cgctacgacg agccgcagga cgccatcgag gcgtggaagg ccggccgcac cgggtacccg
    11281 atcgtcgacg ccgggatgcg tcagctgctc gccgagggct ggatgcacaa ccgggtgcgg
    11341 atgatcgcgg gcagcttcct caccaaggac ctgcacgtgt ggtggccgct cggggcccgg
    11401 cacttcatgg agcacctcat cgacggcgac atcgcctcca acaaccacgg ctggcagtgg
    11461 gtggccggca ccggcaccga cgcctcgccg tacttccggg tgttcaaccc ggtgacgcag
    11521 gggctgcgct tcgaccccga tggtgactac gtgcggcggt gggtgcccga gctcgcgcac
    11581 gtgccgggtg ccgcggccca cgagccctgg gacgtcgccg acggctacga ccacggttac
    11641 ccggcgcgga tcgtcgacca cgcggccgag cgcatcgaag ccctcgaccg cctccaggcc
    11701 accaagtcct gacccctgat agtcggggtg ggggtggtgg ctgcggtgca ataagtcgcg
    11761 gaacggtgtg gatgccgtgt ctaaggttta cgtccttttc cgcaccttcg ggttgtgaaa
    11821 ctcgccaggg attccctacc cttgctcgaa cgaggggagt acttcccaag ctcgtgccgg
    11881 tcaatacgtt cacccccgag gtgaactcgg tacggcgccg accggcatcc gggcggtgaa
    11941 ggagacctca agaccgcagt tctgtcttga ggagacccgt gttcacgtcc ttgccctcgt
    12001 ccctgctcgc agccggagca cctgccgctg ccgagcccgc gaccgccatc cagtcgatcg
    12061 gcacgcccac gctctgggca ctgaccatcg gcgcggtcat cgtgctggtc atcgtcgact
    12121 tcctcatcac ccgcaggccg cacgaggtct ccatgaagga ggccctgggc tggtcggcgt
    12181 tctacatcgc catccc
//
LOCUS       DAOSOC010000065        12068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5251_length_12068_cov_8.433447, whole genome shotgun sequence.
ACCESSION   DAOSOC010000065 DAOSOC010000000
VERSION     DAOSOC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5251_length_12068_cov_8.433447"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PL014_06640"
     CDS             <1..500
                     /locus_tag="PL014_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT56802.1"
                     /translation="LDRMDRVIEIARFMADSRSVLFLGRNVGYPIALEGALKLKEIAY
                     IHAEGFAAGELKHGPIALIEPGQPVFIVVPGPDTPHELHKKVVSNIQEIRARGARTLV
                     IAEDGDEDVVPFADEVIRVPATLPLLMPLLTIVPLQIFALHLATAKGLDVDQPRNLAK
                     SVTVE"
     gene            507..884
                     /locus_tag="PL014_06645"
     CDS             507..884
                     /locus_tag="PL014_06645"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOT56803.1"
                     /translation="MIVGVGIDVVDVARFGAALERTPSLRDRLFTPREREMALNSKAA
                     RFAAKEALAKALGAPAGMHWQDAEVHRGPDGRPHFEVRGTVDARVQALGITSIHVSLS
                     HDAGIASAVVVAEGPGASLESSA"
     gene            881..2404
                     /locus_tag="PL014_06650"
     CDS             881..2404
                     /locus_tag="PL014_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT56804.1"
                     /translation="MNRAWSTDAVYGAEAALMATLPDGELMARAVEGLALVAAARLQE
                     CDGAAVAALVGPGNNGADALYAVARLAEAGWNAVALHDEVVHEGARAAAEEAGVLLTT
                     DPAALAEADVVLDGILGIGARPGLPPWAAAWVAAVPDTAYVIAVDLPSGQDPMGGALD
                     PDGVFADETVTFSVPKPVHLLPPTEQACGILTVVDIGLELAADPVVLRLDHDDVAALW
                     PVPTPADDKYSRGVVGVVAGGEAYTGAAVLAVTAAVESGAGMVRYVGTPTPSALVRAA
                     VPEAVHGPGRVQAWVIGPGLDVEATGADAAAQLEVARAALAGDEPVVLDAGGLDLVDA
                     DFVRSRAGRATLLTPHAGECARLLSRLNAVAGSVVPGVTYWSISGQGEVTRGQVEASP
                     VAHAKALAVATGATVLLKGSTTVVVGPGEGAPAWSQADAPAWLGTAGAGDVLAGLLGT
                     LVAAGLPPDSAGALGALVHGVAADRANPGGPVRALRVAHGIPDAVAHLLGRATNRSS"
     gene            2418..3584
                     /gene="alr"
                     /locus_tag="PL014_06655"
     CDS             2418..3584
                     /gene="alr"
                     /locus_tag="PL014_06655"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT56805.1"
                     /translation="MTTPPAPVPAPAGASAWVSIDLDAIRDNVAELVRRAAPAQVLAV
                     VKADAYGHGLVDSARAALAGGASWLGVAQLAEALRLREAGITAPLLTWLFAPGADIGG
                     AIDADIEVTVGSMWSLDAVLAAVRERGAPARIQVKVDTGLGRGGILTDWPQMVARLAA
                     AQAEGAVEVTGVWSHFAWADAPQHPTVRAQQVRFEEACDELARAGVRPPLRHLANSAA
                     TLTNPSAHFDLVRPGLAVYGLSPVPDLGDSAHFGLREAMRVTARLSVVKRAAAGQGVS
                     YGHEYVTDRDTVLGLVPMGYADGIPRAAGGAGPMLVGDRRLTIAGRVCMDQVVLDLGP
                     DFAGAAGDEVVVLGRAADGEPSAQDWAQAAGTINYEIVTRMSPRLPRVLLGGPF"
     gene            3611..4750
                     /locus_tag="PL014_06660"
     CDS             3611..4750
                     /locus_tag="PL014_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT56806.1"
                     /translation="MATVASAVGERVTRARAGYRSVDAPDGWAHTPTEALIVTTEDGV
                     ALHVEIDAPDTTSLSRRHLAGRTPTVVLVHGFALTMQSWVLQRRSLKHSGFRVVSYDQ
                     RGHGRSGMPDLERCTIDQLGRDLAAVLDATCTSGPVVLVGHSMGGMTIMSYAGQHRDV
                     VRDRVLAVALVSTSPGGDEMTEFGLGTTAGRVVGSFGPTVLTRLSRHAGPIGALRKMG
                     KGVQDAAVQRWAFDSPVSTDLVQLVGDMIFGTSFDVMAAFLPDMDALDISGELTALVG
                     VETLVINGDGDLITPPSHSEEIVRRIPGAEHVVVEDAGHILQLEHPQVVTQQLLMLIG
                     RAQRAVAEGLPVSDKPRVRRTVQDISKKRRVARAKSRRAGREAAS"
     gene            4747..5241
                     /gene="tsaE"
                     /locus_tag="PL014_06665"
     CDS             4747..5241
                     /gene="tsaE"
                     /locus_tag="PL014_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT56807.1"
                     /translation="MTARQIVLPGVEDTQALGRALGALLRAGDLVVLTGGLGAGKTTL
                     TQGLGDGLGVRGPVTSPTFVIARVHPSLVGGPVLVHVDAYRLGGALELDALDLDASVE
                     DGVTVVEWGQGLAEDLSENRLEVVLDVDPATEVRTVTVTGLGGRWTSDDAAAALAALG
                     GEPC"
     gene            5235..5894
                     /gene="tsaB"
                     /locus_tag="PL014_06670"
     CDS             5235..5894
                     /gene="tsaB"
                     /locus_tag="PL014_06670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT56808.1"
                     /translation="MLILGIDTSTSAIGAGLSDGLGVVAAAHHVDARGHTEQLAPLIA
                     TLLADNDLTPGDLTGVVVGNGPGPFTGLRVGMVTGVTMAHALGIPVFGLCSLDVLAHQ
                     GAAATHGEILVATDARRKEVYWARYRIESDAVERLSGPAVDRPAELPEDVRALPTLGR
                     GPVLYPDLFPQAVEILDVDAGVLALVGARGIVAGSPMPVEPLYLRRPDALTSAQRGAT
                     S"
     gene            5891..6358
                     /gene="rimI"
                     /locus_tag="PL014_06675"
     CDS             5891..6358
                     /gene="rimI"
                     /locus_tag="PL014_06675"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOT56809.1"
                     /translation="MSTLRDVHWTDIEQLAQLERELFADDAWSQQTWWAELAGRPRRD
                     YVAEVEGAEVLGYAGLDHGGDVADVMTIAVAPSARGRGLGRRLLDELETRARAGRAAS
                     VMLEVRADNAAAIGLYDRAGYTVVSTRRRYYQPGDIDALVMRKILSDGEVGGE"
     gene            6351..7394
                     /gene="tsaD"
                     /locus_tag="PL014_06680"
     CDS             6351..7394
                     /gene="tsaD"
                     /locus_tag="PL014_06680"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT56810.1"
                     /translation="MSDQPLVLGIETSCDETGVGIVRGETLLVDAIASSVDEHARFGG
                     VVPEVASRAHLEAMVPTIERACTTAGVSLRDLDAVAVTSGPGLAGALMVGVASAKALA
                     LSLGVPLYGVNHLASHVAVDIVEHGPLPEPTLALLVSGGHSSLLLVPDVTHDVRSLGS
                     TIDDAAGEAFDKVARVLGLPFPGGPHIDRAARDGQVVIDFPRGLTQGRDMERHRFDFS
                     FSGLKTAVSRWVQAREAAGEPVPVADVAASFQEAVTDVLTRKAVLACREHGVENLQIG
                     GGVAANSRLRAMAQERCDAAGIELRVPRPGLCTDNGAMVAALGAQMVLKGRPESDLSL
                     PADSSMPVTLVQS"
     gene            complement(7405..8004)
                     /locus_tag="PL014_06685"
     CDS             complement(7405..8004)
                     /locus_tag="PL014_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT56811.1"
                     /translation="MGPGLRERKRAALERDLANAALTLVESRGFSAVTVDDIVEMTGV
                     SRRTFSNYFACKEDAVAAVVLHRAGDALESWRPATTHDGLVPVVRSLIAHQRRSGTFA
                     TMAAVSRLARGHHQLVPYLREAQWSLWTMAGARVLDELGETDDRSRAELTALMGAVFG
                     VVTSSFLSPSRDGDEDDVPMAPPHLEHVLTRLERGFTAP"
     gene            complement(8018..10702)
                     /locus_tag="PL014_06690"
     CDS             complement(8018..10702)
                     /locus_tag="PL014_06690"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT56812.1"
                     /translation="MSFVLYRLGRYSFDHPLRILGVWLLVIAAVVGLLVTQPRVISSG
                     MTLDDTPSQRVMDSISEAMPQAEGTQGSLVFTSADGARIDTTERAAAVVAALGAARGT
                     GFVVDRDEKLEEQRAEVAERILDQVETKVAAELAPQLAALATSLDAVAAAVPPGQPDA
                     SALVARLDELADRARTLSTAPPGDQVDGSTVLFADLEELTVRLEATGAAESLTLRAPS
                     GEMSDPRARVDEAVAEATTAALADLERLTAGTSPRGEPLTVGGTSYGSVLVSTDGRTA
                     VASVQLTGQVSDLPEGAVEAMLDEVGAASGAAGVEVSATSSLLPAEPPLGGHEALGLV
                     IAAVVLILALGSLVAAGLPLLTALAGVFIGVGGAFALSANFHMTTSTPALGLMIGLAV
                     GIDYALFVVHKHRSLMIRSDLAARDAVGEAVATAGSAVLFAGLTVVIALLGLLTLGIA
                     FVNTMALTAATTVTLAVLIALTALPALLGLVGERIATSRARLRGRRPGRQRIAHGWVS
                     GVTRRPWLTVLGVVALLGVAALPAPGLDLGMPSGAVAQVDSPQRTSYDAITEAFGEGT
                     NAPLIVLAREIELAGFDQETLLSTQQGLADTDGVVDVALMGVSPDNSLAIYQVTPEGG
                     PNDESTADLVTRLRSVPLPDVGLLGVTGLTAMNIDLSEALAAAIPGYVVLVVVLSLLV
                     LLVVFRSVLVPLAATFGFLLTMAAVAGLVVTAFGNPDWTWLVGVDRPGPILPFLPIMA
                     TGILYGLANDYQLFLGTSIREEYVRGSDARTAIRGGFVHAGRVVVAAAIIMVSVFGGF
                     VLSSDTTIRQFGFALSVGILIDAFLIRMTLIPALLHIAGERAWWLPRWLDAILPAVDI
                     EGARLRQRGTTSSDPPELAPAEPAGSPPAEPVVVVGRP"
     gene            complement(10906..11322)
                     /locus_tag="PL014_06695"
     CDS             complement(10906..11322)
                     /locus_tag="PL014_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56813.1"
                     /translation="MAHHHGSPGVQAELAQVRAATARFHRVEEAVAAGYELGWVNGAG
                     NRIVAGCVSHPTAGAMGYHYFNAELMADNAVDALEPEVLVYAPAADGGLRLVAGPSRR
                     PRRPEGGWGAPTPRSVVGPALIARRTPGRSRRNCRP"
     gene            complement(11583..>12068)
                     /locus_tag="PL014_06700"
     CDS             complement(11583..>12068)
                     /locus_tag="PL014_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOT56814.1"
                     /translation="LGRVRNNTDFSSTGITDSVATTKDPFLQPFADGIEAGAGLVMMA
                     SARYPKLDDANPAMFSEKIVTGLLREQLGYEGVVITDDVNAKALADVPTGARAVRHVA
                     AGGDIVLTGAASDAGTMLEALAAKAAADAGFAATVDASVERVLTLKERMGLLPCSTTK
                     P"
BASE COUNT         1627 a         4288 c         4471 g         1682 t
ORIGIN      
        1 tgctcgaccg catggaccgg gtcatcgaga tcgcccgctt catggccgac agccgatcgg
       61 tgctcttcct cgggcgcaac gtcggctacc cgatcgcgtt ggagggcgca ctcaagctca
      121 aggagatcgc ctacatccac gccgagggct tcgcggccgg tgagctcaag cacggcccga
      181 tcgcgctcat cgagcccggc cagccggtgt tcatcgtcgt gcccggcccg gacaccccgc
      241 acgagctgca caagaaggtg gtctccaaca tccaggagat ccgtgctcgc ggcgcccgca
      301 cgctcgtcat cgccgaggac ggcgacgagg acgtcgtgcc cttcgccgac gaggtgatcc
      361 gggtgcccgc gacgctgccg ctgctcatgc cgctgctgac gatcgtgccg ctgcagatct
      421 tcgcgctgca cctggccacg gcaaaggggt tggacgtcga ccagccgcgc aacctcgcca
      481 agagcgtcac cgttgagtga gcagtcgtga tcgtcggggt gggcatcgac gtcgtcgacg
      541 tggctcgctt cggcgcggcg ctcgagcgca ccccgtccct gcgtgatcga ctcttcacac
      601 cgcgcgagcg cgagatggcg ctgaactcca aggcggcgcg gttcgccgcc aaggaggccc
      661 tggccaaggc cctgggtgca cctgccggca tgcactggca ggacgccgag gtccaccgcg
      721 gccccgacgg ccgcccgcac ttcgaggtgc gcggaaccgt cgacgcacgg gtgcaggcgc
      781 tggggatcac ctcgatccac gtctcgctct cgcacgacgc cgggatcgcc tcggcggtgg
      841 tcgtcgccga gggacccggt gcttcactgg agtcgtcggc gtgaaccggg cgtggtcgac
      901 ggatgccgtg tacggcgccg aggcggcgct catggccacg ctgcccgacg gtgagctgat
      961 ggcccgggcg gtcgaggggc ttgccctcgt ggcggccgcc cgcctccagg agtgcgacgg
     1021 tgcggcagtc gccgccctcg tcgggcccgg caacaacggc gctgacgccc tgtacgcggt
     1081 ggcgcggctg gccgaggccg gatggaacgc ggtcgccctg cacgacgagg tcgtgcacga
     1141 gggggcacgg gccgccgccg aggaggctgg ggtcctgctc acgaccgacc cggcagcgtt
     1201 ggccgaggcc gacgtggtgc tcgacggcat cctcggtatc ggggcacgcc cgggcctgcc
     1261 gccgtgggct gctgcctggg tggccgcagt gcccgacacg gcatacgtca tcgccgtcga
     1321 ccttccctcg ggacaggacc cgatgggcgg ggcgctcgac cccgacgggg tcttcgccga
     1381 cgagacggtg accttctcgg tgcccaagcc ggtgcacctg ctgccgccca ccgagcaggc
     1441 gtgcggcatc ctcaccgtcg tcgacatcgg cctcgagctg gccgccgacc cggtggtgct
     1501 gcgcctcgac cacgacgacg tcgcggccct gtggccggtg ccgacaccgg ccgatgacaa
     1561 gtactcgaga ggcgtcgtcg gggtcgtcgc cggtggtgag gcgtacacgg gggctgcggt
     1621 gctcgccgtc acggcggccg tcgagtccgg tgcgggcatg gtgcgctacg tcggcacgcc
     1681 caccccgagc gccctcgtgc gcgcggccgt gcccgaggcc gtccacgggc ccggccgggt
     1741 gcaggcctgg gtgatcggac cggggctcga cgtcgaggcc accggcgccg acgccgctgc
     1801 ccagctcgag gtggcgcgtg ccgctcttgc cggcgacgag ccggtcgtcc tcgatgccgg
     1861 cgggctcgac ctcgtcgatg ccgacttcgt gcggtcgcgg gccgggcgcg cgacgctgct
     1921 gaccccgcac gccggggagt gcgcgcgcct gctcagccgc ctgaacgcgg tcgcaggctc
     1981 ggtcgtacct ggagtgacct attggtcgat ttcgggacag ggggaggtga cgcgggggca
     2041 ggtcgaggcc tccccggtcg cccacgccaa ggccctcgcc gtcgccaccg gcgccaccgt
     2101 cctgctcaag ggctcgacca ccgtcgtcgt cgggcccggt gagggcgccc cggcctggtc
     2161 ccaggctgac gcgcccgcct ggctcggcac cgccggtgcg ggcgacgtcc tcgccgggtt
     2221 gctcggcacg ctcgtggccg ccgggctgcc gcccgactcg gccggggcgc tcggcgccct
     2281 cgtgcacggc gtcgctgcgg accgcgccaa cccgggaggc ccggtgcggg cactgcgggt
     2341 cgcccacggc atcccggatg ccgttgctca cctgctgggt cgcgccacca accgctcgtc
     2401 ctagactcaa caggcccatg acaactcctc ccgctcccgt gcccgcgccc gcgggtgctt
     2461 cggcgtgggt gagcatcgac ctcgacgcga tccgcgacaa cgtcgccgaa ctcgtgcgcc
     2521 gggccgcccc ggcgcaggtg ctggccgtcg tcaaggccga cgcctacggt cacgggctgg
     2581 tcgacagcgc gcgcgccgcg ctcgcgggtg gcgcctcgtg gctcggcgtg gcccagctcg
     2641 ccgaggccct gcgcctgcgc gaggcaggga tcacggcccc gctgctcacc tggctcttcg
     2701 cgcccggcgc cgacatcggt ggggccatcg acgccgacat cgaggtgacc gtcgggagca
     2761 tgtggtcctt ggatgccgtc ctcgcggccg tgcgtgagcg tggcgctccc gcacgcatcc
     2821 aggtcaaggt cgacaccgga ctcgggcgcg gcggcatcct caccgactgg ccgcagatgg
     2881 tggcgcgcct cgccgcggcg caggccgagg gcgcggtcga ggtgaccggc gtgtggtcgc
     2941 acttcgcgtg ggccgatgca ccgcagcacc cgacggtgcg ggcccagcag gtgcgcttcg
     3001 aggaagcctg cgacgagctt gctcgtgccg gggtgcgacc cccgctgcgc cacctggcca
     3061 actccgcggc gacgctgacc aacccgagcg cccacttcga cctcgtgcgg cccgggctcg
     3121 cggtctacgg gctctcaccg gtgcccgacc tcggcgacag cgcgcacttc ggcctgcgtg
     3181 aggcgatgcg ggtcacggct cgcctctccg tggtgaagcg ggcggctgcg gggcaagggg
     3241 tcagctacgg gcacgagtac gtcaccgacc gcgacaccgt gctcggcctc gtgccgatgg
     3301 ggtatgccga cggcatcccg cgtgcagccg ggggagccgg gccgatgctc gtcggcgatc
     3361 ggcgcctcac catcgcgggg cgggtgtgca tggaccaggt cgtcctcgat ctcggccccg
     3421 acttcgcggg ggcggccggc gacgaggtcg tcgtgctcgg gcgcgcggcc gacggcgagc
     3481 cctcggcgca ggactgggcg caggctgccg gcaccatcaa ctacgagatc gtcacccgga
     3541 tgagccctcg cctgccgcgg gtcctgctcg gggggccgtt ctgatggcgc ggttcggacc
     3601 actcgggccg gtggccacgg tcgcgagcgc cgtgggtgaa cgggtcacgc gggcgcgcgc
     3661 cggctaccgg tcggtggacg cccccgacgg ctgggcgcac accccgaccg aggccctcat
     3721 cgtcaccacc gaggacggcg tggccctgca cgtcgagatc gatgcccccg acacgacgag
     3781 cctgagtcgg cgccacctcg cgggccgcac tccgacggtg gtgctcgtgc acgggttcgc
     3841 cctgaccatg cagtcctggg tcctgcagcg ccgctcgctg aagcactcgg gcttccgggt
     3901 cgtcagctac gaccagcgcg gccacggtcg ctccggcatg cccgacctcg agcggtgcac
     3961 catcgaccag ctcgggcgcg acctggctgc cgtgctcgac gccacctgca cgtccgggcc
     4021 ggtggtgctc gtcgggcact cgatgggtgg catgacgatc atgtcctacg cggggcagca
     4081 ccgcgacgtc gtgcgcgacc gcgtgctcgc ggtggccctg gtctcgacga gccccggcgg
     4141 cgacgagatg accgagttcg ggttgggcac gacggcggga cgggtcgtcg gctcgttcgg
     4201 gccgaccgtg ctgacccggc tctcgcggca cgccggcccg atcggtgccc tgcgcaagat
     4261 gggcaagggt gtgcaggatg ccgcggtgca gcggtgggcg ttcgactcgc cggtgagcac
     4321 cgacctcgtc cagctggtcg gggacatgat cttcgggacg tccttcgacg tcatggctgc
     4381 cttcctccca gacatggacg ccctcgacat cagtggggag ctgacggccc tcgtcggcgt
     4441 cgagacgctg gtcatcaacg gcgacggtga cctcatcacc ccgccgtcgc acagcgagga
     4501 gatcgtgcgt cgcatccccg gggccgaaca cgtcgtcgtc gaggacgccg ggcacatcct
     4561 gcagctcgag cacccgcagg tggtcacgca gcagctgctc atgctcatcg gccgcgccca
     4621 gcgcgccgtc gccgaagggt tgccggtcag cgacaagccg cgggttcgcc gcaccgtgca
     4681 ggacatctcc aagaagcgtc gggtggcgcg ggcgaagtcc cgccgggccg gtcgcgaggc
     4741 ggcgtcgtga ccgcgcggca gatcgtgctg ccgggggtcg aggacaccca ggccctgggg
     4801 cgtgcgctcg gtgccctcct gcgtgcgggg gacctggtcg tgctgaccgg tggcctgggt
     4861 gcgggaaaga ccaccctcac ccaggggctc ggtgacgggc tcggggtgcg gggcccggtg
     4921 acctcgccga cgttcgtcat cgcccgggtg cacccctcgc tcgtcggggg cccggtgctc
     4981 gtgcacgtcg acgcctaccg actcggcggc gcgctcgagc tcgacgctct cgacctcgat
     5041 gcctccgtcg aggacggcgt caccgttgtc gagtgggggc aggggctggc cgaggacctc
     5101 agtgagaacc ggctcgaggt cgtgctcgac gtcgacccgg ccaccgaggt gcgcaccgtg
     5161 accgtgaccg gcctcggcgg gcgctggacg tccgacgacg cagctgccgc cctggcggcc
     5221 ctgggagggg agccgtgctg atcctcggca tcgacacgtc gacgtccgcg atcggggccg
     5281 ggctcagcga cggcctcggg gtcgtcgcgg ccgcgcacca cgtcgatgcc cgcgggcaca
     5341 ccgagcagct cgcgccgctc atcgcgacgc tgctcgcgga caacgacctc accccgggcg
     5401 acctcaccgg ggtcgtcgtc ggcaacggtc cggggccgtt cacgggcctg cgggtcggca
     5461 tggtgacggg cgtgacgatg gctcacgccc tcggcatccc ggtgttcggg ctgtgctcac
     5521 tcgacgtgct ggcccatcag ggggctgcgg cgacgcacgg cgagatcctc gtcgcgaccg
     5581 atgcgcggcg caaggaggtc tactgggccc gctaccgcat cgagtcggat gccgtggagc
     5641 gcctgtcggg gccggccgtc gaccgccccg cggagctgcc cgaggacgtg cgtgccctgc
     5701 cgacgctggg tcgcggcccg gtgctctacc cggacctgtt cccgcaggcc gtggagatcc
     5761 tcgacgtgga tgccggtgtc ctcgcgctcg tcggtgcccg gggcatcgtc gccgggtcgc
     5821 cgatgccggt cgagccgctc tacctgcgcc gccccgacgc cctgaccagc gcgcagcggg
     5881 gagccacctc gtgagcaccc tgcgtgacgt gcactggacc gacatcgagc agctggcgca
     5941 gctggagcgc gagctgttcg ccgacgacgc ctggtcgcag cagacctggt gggccgagct
     6001 cgccgggcgt ccgcgccgcg actacgtggc cgaggtcgag ggtgctgagg tgctcgggta
     6061 cgccggcctc gaccacggcg gcgacgtggc cgacgtcatg acgatcgcgg tcgcaccgtc
     6121 ggcacggggc cggggcctgg gtcggcgact gctcgacgag ctcgagacgc gagcgagggc
     6181 cgggcgggcg gcatccgtca tgctcgaggt gcgcgccgac aacgccgcgg ccatcgggct
     6241 ctacgaccgg gcggggtaca ccgtggtcag cacgcgccgg cgctactacc aaccgggcga
     6301 catcgacgcc ctcgtgatgc gaaagatcct gtccgacggg gaggttggcg gtgagtgacc
     6361 agccgttggt gctcggcatc gagacgtcgt gcgacgagac cggggtcggc atcgtgcggg
     6421 gcgagacgct gctcgtcgac gcgatcgcga gcagtgtcga cgagcacgcc cgcttcgggg
     6481 gagtggtgcc cgaggtcgcg agccgcgccc acctcgaggc gatggtgccg acgatcgaac
     6541 gcgcctgcac cactgcggga gtgagccttc gtgacctgga tgccgtcgcc gtcacctccg
     6601 ggccggggct ggccggtgcg ctcatggtgg gggtggcctc ggccaaggcg ttggcgctct
     6661 cgctcggggt gccgctctac ggcgtgaacc acctcgccag ccacgtcgcc gtcgacatcg
     6721 tcgagcacgg gccgttgccc gagccgacct tggcgctgct ggtgtccggg gggcactcct
     6781 cgctgctgct cgtgcccgac gtcacgcacg acgtgcgcag cctggggtcg acgatcgacg
     6841 acgcggcggg ggaggcgttc gacaaggtgg cccgggtgct cgggttgccc ttccccggcg
     6901 gtccgcacat cgaccgggcg gcgcgtgacg ggcaggtcgt catcgacttc ccgcgcgggt
     6961 tgacccaggg gcgcgacatg gagcggcacc ggttcgactt ctcgttctcg gggctgaaga
     7021 cggccgtgtc gcgctgggtg caggcccggg aggctgccgg tgagccggtt cccgtcgccg
     7081 acgtggccgc gagcttccag gaggcggtca ccgacgtgct gacccgcaag gccgtgctcg
     7141 cctgccggga gcacggggtg gagaacctgc agatcggcgg cggggtcgcg gccaactcgc
     7201 ggctgcgggc catggcccag gagcgctgtg atgccgcggg catcgagctg cgggtgccgc
     7261 gaccggggct gtgcaccgac aacggggcga tggtggcggc gctgggagct cagatggtcc
     7321 tgaaggggcg tcccgaatcc gacctgtcgt tgccggccga ctcctcgatg ccggtcaccc
     7381 tcgtccagtc ctgaccactg ccgactacgg cgcggtgaag ccgcgctcca gtcgggtgag
     7441 gacgtgctcg aggtgtggtg gcgccatcgg gacgtcgtcc tcgtcgccat cacgtgaggg
     7501 ggagaggaag gagctggtca cgacgccgaa caccgcgccc atgagggcgg tgagctcggc
     7561 cctgctccgg tcgtcggtct cgccgagctc gtcgagcacc ctggcgccgg ccatggtcca
     7621 cagcgaccac tgggcctcgc gcaggtacgg gaccagctgg tggtgaccac gcgcgagccg
     7681 ggacactgcg gccatcgtcg cgaacgtccc cgagcgcctc tggtgggcga tgagggagcg
     7741 caccacgggc accaggccgt cgtgcgtcgt cgccggccgc cagctctcga gtgcgtcgcc
     7801 cgcacggtgg aggaccaccg ccgcgacggc atcctccttg cacgcgaagt agttcgagaa
     7861 ggtccgtcgg gacacccccg tcatctcgac gatgtcgtcg accgtgaccg cggagaaccc
     7921 acgcgactcc accagtgtca gggcggcatt cgccaggtcg cgttcgagcg cggcgcgctt
     7981 gcgttcccga agccccggtc ccatggcagg cactatgtca cggccgcccg accaccacga
     8041 cgggctccgc cgggggactg cctgcgggct cggcaggggc gagttccggc gggtcggagc
     8101 tcgtcgttcc gcgctggcgg agccgagccc cctcgatgtc gaccgcgggg aggatggcgt
     8161 cgagccaccg gggcagccac caggcccgct cacccgcgat gtgcagcagc gcggggatga
     8221 gagtcatgcg gatgaggaac gcgtcgatga ggatgcccac ggacagggcg aagccgaact
     8281 ggcggatggt cgtgtcactg ctgaggacga agccgccgaa gacggagacc atgatgatcg
     8341 cggcggcgac gacgacgcgt cccgcgtgga cgaacccgcc gcggatcgcg gttcgggcgt
     8401 cggagccgcg gacgtactcc tcccggatcg acgtcccgag gaagagctgg tagtcgttgg
     8461 cgagcccgta gaggatgccg gtcgccatga tcggcaggaa gggcaggatc ggtcccggtc
     8521 ggtccacgcc gacgagccag gtccagtccg ggttgccgaa ggcggtcacg acgagaccgg
     8581 cgaccgccgc catcgtcagg aggaacccga aggttgcggc gagcggcacg agcactgagc
     8641 ggaacacgac gaggaggacg aggagggaga gcacgacgac gaggacgacg tagccgggga
     8701 tcgcggcagc gagagcctcg gagaggtcga tgttcatcgc cgtcagcccc gtgactccga
     8761 ggaggccgac atcggggagc ggcaccgacc gcagccgcgt cacgaggtcg gcagtggact
     8821 cgtcgttcgg cccgccctcg ggtgtcacct ggtagatcgc gagcgagttg tcgggggaga
     8881 cgcccatcag cgcgacgtcc accacgccgt cggtgtcggc caggccctgc tgggtcgaga
     8941 gcagcgtctc ctggtcgaag cccgccagct cgatctcgcg cgccaggacg atgagggggg
     9001 cgttcgtccc ctccccgaac gcctcggtga tagcgtcgta cgacgtgcgc tgcggcgagt
     9061 ccacctgggc gacggcaccg gacggcatcc cgaggtcgag cccgggggcg ggcagtgcgg
     9121 cgaccccgag gagcgcgacg accccgagca ccgtcagcca cggtcggcga gtgacccccg
     9181 agacccagcc gtgggcgatg cgctgacgcc ccggtcggcg ccctcgcagg cgggcccggc
     9241 tggtcgcgat gcgctcgccg acgagcccga ggagggccgg aagtgcggtg agggcgatga
     9301 gcacggccag ggtgacggtc gtcgcggccg tgagcgccat ggtgttgacg aaggcgatgc
     9361 cgagcgtgag caggccgagg agggcgatga ccacggtgag ccccgcgaac agcacggcgc
     9421 ttcccgcagt ggcaaccgcc tcgcccactg cgtcacgcgc ggccaggtcg gagcggatca
     9481 tgaggctgcg gtgcttgtgc acgacgaaca gggcgtagtc gatgcccacc gcgagcccga
     9541 tcatcagccc cagggcgggc gtcgaggtcg tcatgtggaa gttcgccgac agggcgaagg
     9601 ctccgcccac gccgatgaac accccggcga gggccgtcag cagcggaagg ccggcggcga
     9661 ccagcgaccc gagggcgagg atgaggacga cggctgcgat cacgagcccg agggcctcat
     9721 ggcccccgag cggcggttcg gccggcagca gtgacgaggt cgccgagacc tccacaccgg
     9781 ctgcgccgga agccgctccg acctcgtcca gcatcgcctc gacagccccc tcgggcaggt
     9841 cggagacctg cccggtgagc tgtaccgacg ccaccgccgt gcggccgtcc gtcgagacga
     9901 gcacgctgcc gtaggaggtg ccgcccaccg tgagcggctc gcctcggggg ctggtcccag
     9961 ccgtgagccg ctcgaggtcg gcgagcgcgg ccgtcgtcgc ctccgccacc gcctcgtcga
    10021 cgcgggcccg cggatccgac atctcgcccg acggggcccg cagcgtcagc gactcggctg
    10081 cgccggtggc ttcgagacgg acggtcagct cctcgagatc ggcgaagagc accgtggatc
    10141 cgtcgacctg gtcgcccggc ggggcggtcg agagggtccg agcccggtct gccagctcgt
    10201 cgagccgcgc gacgagggcc gaggcgtccg gctggccggg gggcaccgca gcagcaaccg
    10261 cgtcgagcga ggtcgccaac gcagccagct gcggggcgag ctccgcagcg accttggtct
    10321 cgacctggtc caggatgcgc tcggcaacct cggcgcgctg ttcctcgagc ttctcgtccc
    10381 ggtcgacgac gaagccggtg ccccgggcag cccccagcgc ggcgacgacg gcagcggcgc
    10441 gctcggtggt gtcgatccgg gcgccgtcgg cgctcgtgaa caccaggctc ccctgcgtcc
    10501 cctcggcctg cggcatcgcc tcgctgatcg agtccatcac ccgctgggag ggggtgtcgt
    10561 cgagcgtcat cccgctggag atgacccgcg gttgcgtgac cagcaggccc accaccgccg
    10621 cgatcacgag gagccacaca ccgaggatgc gcagcgggtg atcgaaggag taccgcccga
    10681 ggcggtagag cacgaaggac acgacggcaa ctccaagggg atgggcaggt aggaaccctc
    10741 acggtagggg aagaatgcgc aaagagcaat gttgcctatt gggcagataa gccacctgcc
    10801 tgcgccaccg gcgctgtggg cgggtccgtg accgtcggga ccgtcacgtc cggcggcagc
    10861 tcgtgcccga ggctggggcc tggccgctcg tttcctagac tcacgtcatg ggcgacagtt
    10921 gcgtctcgag cgtcccggtg ttcgccgggc tatcagcgca ggaccaacga cggatcgcgg
    10981 ggttggcgca ccccacccac cttcgggccg gcgaggtcgc ctactcggcc ccgccacgag
    11041 cctcagcccg ccatcggccg ccggggcgta gaccaggacc tcgggctcga gggcgtcgac
    11101 ggcgttgtcg gccatcagct cggcgttgaa gtagtggtac cccatcgccc ctgccgtcgg
    11161 gtgcgacacg cagcccgcga cgattcggtt gcctgcaccg ttgacccagc cgagctcgta
    11221 gccggccgcg accgcctcct ccacccggtg gaaccttgct gtcgccgccc gcacctgggc
    11281 cagctcggcc tgcactccag gactgccgtg gtggtgggcc atgggctgta cctcgccgga
    11341 tgcgaccgat gggaacagtg ctgcccctgc gagcagcggc cccagcaggc ccaggcacag
    11401 gccggagccc accagagcct tccgaccgtg tcggctgctg cgttccgtcg agcctgtgtc
    11461 gttcgccatc tctgcacctc tgttcgtcgc caagaccggg ttgttcgggg aggactccct
    11521 acccccttga cgcctcgacg cttcccgtcg gttcagtggc tgtgctcggc cgctccagcc
    11581 ggtcagggct tcgtggtcga gcagggcagc aggcccatcc gctccttgag ggtgagcacc
    11641 cgctcgaccg aggcgtcgac cgtggcggcg aacccggcat ccgccgccgc cttcgcggcc
    11701 agcgcctcga gcatggtgcc ggcatcggat gccgccccgg tcagcacgat gtcgcccccg
    11761 gccgcgacgt ggcgcaccgc ccgggccccg gtcgggacgt cggccagggc cttcgcgttg
    11821 acgtcgtcgg tgatgacgac gccctcgtag cccagctgct cgcgcagcag cccggtgacg
    11881 atcttctccg agaacatcgc cgggttggcg tcgtcgagct tggggtaccg ggccgaggcc
    11941 atcatcacca agcccgcgcc ggcctcgatg ccgtccgcga acggttggag gaacggatct
    12001 ttcgtcgtgg caacggaatc cgtgatgccg gtcgacgaga agtcggtgtt gttgcgcacc
    12061 cgacccag
//
LOCUS       DAOSOC010000066        11989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5287_length_11989_cov_8.978716, whole genome shotgun sequence.
ACCESSION   DAOSOC010000066 DAOSOC010000000
VERSION     DAOSOC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11989
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5287_length_11989_cov_8.978716"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1405
                     /locus_tag="PL014_06705"
     CDS             41..1405
                     /locus_tag="PL014_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOT56815.1"
                     /translation="MFGAAAADAAGADHPASIAPLAVRMRPRTLAEVRGQGDVLRQGS
                     PLRRLIEGSAGAAGPISAILWGPPGTGKTTLAHLVATAADREFVELSAVTAGVKDVRA
                     VMQQATRARQLYGRQTVLFLDEIHRFTKAQQDALLPGVENRQVVLVAATTENPSFSVI
                     APLLSRSMLVTLVPLTDGDIADVLASAVADERGLAGAYRLDDDASEHLVRLAGGDARR
                     ALTFLEAAAGVTEDAVAPGAPRPDVLPITLAQCEQAVAHAAVRYDRTGDQHYDVASAL
                     IKSMRGSDVDAALHYLARMLEAGEDPRFIARRIVISASEDVGLGDPTALQTAVAAMHA
                     VAQIGMPEARIILAQAVVHNAIAPKSNAAYVGINEAIADVRAGRGGPVPAHLRGSGYA
                     GAARLGHGKGYVYAHDEPDAVAAQQYLPDDLAGTTDYYRPSDRGFEARLQERWTWLKG
                     RLGR"
     gene            1437..1799
                     /locus_tag="PL014_06710"
     CDS             1437..1799
                     /locus_tag="PL014_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HOT56816.1"
                     /translation="MEWTLGGIAAIIAALAFAYLVVRLAALLGRASTILDETAASLRT
                     TSENVQPTLKGLTDTVTLTNDQLTRVDTITSSVSTMTTNASALTSVAAATVGSPLIKV
                     AAFSYGVRTAVSGLKKAR"
     gene            1799..2155
                     /locus_tag="PL014_06715"
     CDS             1799..2155
                     /locus_tag="PL014_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56817.1"
                     /translation="MRLRTVVLTAAAVGIGYAVWRMPATQRFVATVKEASARREAELR
                     EAIEVAVLTDATTRAPRHASGVSTPGLDVTAGWDDDRRPRTHSAAALGADDPGRSLTP
                     EEAKALLLDPTGERAQ"
     gene            2302..4992
                     /gene="alaS"
                     /locus_tag="PL014_06720"
     CDS             2302..4992
                     /gene="alaS"
                     /locus_tag="PL014_06720"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT56818.1"
                     /translation="METAEIRRRWLAFFEKNGHTVVPSAPLIHDDPNLLFVNAGMVPF
                     KPYFLGQETPLWDRATSVQKCVRTGDIEEVGKTSRHGTFFQMNGNFSFGDYFKEGAIT
                     LAWELVTTSQAEGGYGLDADKIWPTVYEDDDEAFAIWRDTIGIPVERITRRGKLDNYW
                     HMGVPGPGGPCSEIYLDRGPEYGLEGGPAVDEDRYLEFWNLVFMQEELSAVRAKDDFD
                     ISGPLPKRNIDTGMGLERMATLLQGVDNLYEIDEVYPVIARAAEMTGKRYGEHSGQSA
                     GSSHPDDVRLRVVADHVRSALMLIGDGVTPGNEGRGYVLRRMLRRSVRSMRLLGYEDP
                     CLPELLPVSLERMQNSYPELATGFDRIAQIAYAEEDAFRRTLAAGTTILDTAVARTKD
                     AGGSTLAGEQAFALHDTYGFPIDLTLEMAAEQGLEVDRDGFTRLMREQRERARADAKG
                     KKAGHANTEVWRDLRASGATDFRAYEELTSEATVVGLVVDGEAVQEIAPGQQGQVVLD
                     RTPFYAESGGQIADEGIITADGVHLKVIDVQRPVKGLIAHTVEVVTGGLQTGTSVLAQ
                     VDPEWRLSACQAHSGTHVVHAALRQVLGPSALQSGSYNKPGYLRLDFAWGQALSAATR
                     SEIEEVANLAVRRDLPVSATYMPLPKAREIGALALFGETYGEDVRVVEIGGEWSRELC
                     GGTHVQHSSQVGALTLTGESSVGSGVRRVEAFVGMEALRFLARERAIVAELSGLVGAR
                     PDELTDRIGAMVTRLKDAERELERVRKEAVTAAAGNLTEQARDVNGVTFLGHDAGDAG
                     ADDVRTMVMDLRGRLGNQSPVVVAITGAAKGRPVVVVATNEAARARGIRAGELVRVAA
                     TTLGGGGGGKDDIAQGGGQDVAKVGEALSAVEWRVGELAG"
     gene            4992..5510
                     /gene="ruvX"
                     /locus_tag="PL014_06725"
     CDS             4992..5510
                     /gene="ruvX"
                     /locus_tag="PL014_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOT56819.1"
                     /translation="MPEPSGTWIGVDVGTVRVGVARSDPHGILATPVATLARDPDAAV
                     SPQDPDVTAIAGLVTELGAVGVIVGLPRSMSGVEGPSAAKARQYASVVAARIAPVWVR
                     LIDERLTSVDAHRTLRDSGVSGRRQRAVVDQAAAVLILQNALDTQRTTGSPPGEQVRI
                     GRRKPRTKDVNP"
     gene            5507..6676
                     /gene="mltG"
                     /locus_tag="PL014_06730"
     CDS             5507..6676
                     /gene="mltG"
                     /locus_tag="PL014_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT56820.1"
                     /translation="MTQGDVSHTIFGEPEPPPTRRRGHHRHAKRNNRRWLVLLIALAL
                     LGGAAWAAVSVIKPVLSSVFNGGGSETADFPGPGEGAVEIVVTPGETGEDIATTLRDA
                     GVVKTRGAYIDVARSDPERAAAIQPGRYNLLKGMTAAEAFAVLGDPANRITSGTTVRE
                     GLWATETFEVLSKSTGIPVKEYAAAAKDARAIGLPAEAEGNVEGWLAPSTYEFPENST
                     AAEQLAAMVAQTVKVLDTAGVAPKDRENVLILASLVEAEAKLDEDRPKIARVFLNRLE
                     TDGAPTYGLLQSDAAVSYGAQRRSLFPSEAELNDASNPYNTRLIPGLPPGPISNPGAA
                     SIKAAANPADGPWFFFVAVNPITGETKYGVTLDDHNKNVRELTAYCKEKPKDCGL"
     gene            6673..7494
                     /locus_tag="PL014_06735"
     CDS             6673..7494
                     /locus_tag="PL014_06735"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOT56821.1"
                     /translation="MRAAVLGSPIAHSLSPVIHRAGYLAAGLTGWEYTAHEVDADALP
                     GFVGGLDTSWRGLSLTMPLKEVAGALATTVDAVATRSGAVNTLVRRADAGWDATNTDV
                     VGVIEALRPHLPDRVTRALVLGSGATARSAVLALAELGITTLTVRARDTAKAADLLSW
                     ALGLDIGIRNGSVADLGPWVSTRDDVVVSTLPATAAEAVAATVPAAHHGILLDVGYDG
                     WPTPPARAAAAAGMTVVSGLDMLVHQAAEQFRLFTGHDAPVAEMAAAGRAALARR"
     gene            7512..8174
                     /locus_tag="PL014_06740"
     CDS             7512..8174
                     /locus_tag="PL014_06740"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOT56822.1"
                     /translation="MAAALVMGVVGHLTGQQLATGGYRIPEDEAEHAVGRNWWPGLLC
                     GALSAVAAWAVGDLAGWAALPAYLLFAWLSVGLIWIDLDVHRLPVGLVVPTGWGILGL
                     LGVASLASGDRRWLGAVVGFVVMGVAYLVLAVLPGGGVGGGDVRLAPLIGALLGWLGA
                     AHLVIGLAAGFLIGGVAAAALLVLGRAGLKSSIAYGPAMCLGAWVGIAATSRILTVLS
                     GG"
     gene            8199..9431
                     /gene="aroC"
                     /locus_tag="PL014_06745"
     CDS             8199..9431
                     /gene="aroC"
                     /locus_tag="PL014_06745"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT56823.1"
                     /translation="MLRWLTAGESHGPALVATIDGLPAGVVVSSDDLARALARRRLGH
                     GRGARMSFERDDVEFLGGVRHGVTLGSPIAIRVGNSEWPKWQTVMSPDPVAADDLAAA
                     NDINAEKEIARNRPLTRPRPGHADLVGMQKYGFDDARPVLERASARETAARVALGEVA
                     ARFLDQAYGIRLVSHTVAIGAAGVAEDAPLPTADDVAALDADPVRAFDAAGSAAMVAE
                     VDAAHKDGDTLGGVVEVVVTGLPPGLGSHVHWDRRLDARLAGALMGIQAIKGVEVGDG
                     FRTATRRGSVAHDEMERGPDGVIRRRTGRAGGTEGGMSVGGLLRVRAAMKPISTVPRA
                     LQTVDVASDEPARAIHQRSDVCAVPAAGVVAEAMVALVLAEACLEKFGGDSVGETARN
                     HASYLAAIPASMRTWDDE"
     gene            9428..9934
                     /locus_tag="PL014_06750"
     CDS             9428..9934
                     /locus_tag="PL014_06750"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT56824.1"
                     /translation="MSPRVVLIGPPGSGKSTVGAALAARLGLPLHDTDAAIEAAAGRS
                     ISDIFIEDGEPTFRELERAEVARAVEQEDGVLALGGGAPVDPLTEQVLAGQVVVFLDV
                     GIADASKRVGFDQSRPLLAVNPRASWVRLMNERRPVYERVATHRVDTAGRTPDAVAEE
                     IATLLGAS"
     gene            9931..11007
                     /gene="aroB"
                     /locus_tag="PL014_06755"
     CDS             9931..11007
                     /gene="aroB"
                     /locus_tag="PL014_06755"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOT56825.1"
                     /translation="MTAPATVIPVGTSYDVVVGHGVSDRVTDLLSGDVRQVLVVRPES
                     LARLAAPVVHRLREAGLTVHETAVPDNEVAKTAEVAAGLWATLGQHAFTRSDAVVAVG
                     GGTVTDLAGFVAATWLRGVAVVHVPTTLLAMVDAAVGGKTGINTAEGKNLVGSFHEPA
                     GVLCDLDALATLPRPDLVAGLAEVVKAGFIADPRILELVEGDPDGATRADGPAMRELV
                     ERAIAMKAEVVAADLKESWLREILNYGHTLGHAVEQVEGYRRRHGEAVAIGMTYAAHL
                     AHRAGHLTADAVARHRAILTSVGLPTTYEAGHWDALATAMQRDKKTRGSLLRFIVLED
                     IGAPTRLVGPDEALLRAAYDDLCR"
     gene            11094..>11989
                     /locus_tag="PL014_06760"
     CDS             11094..>11989
                     /locus_tag="PL014_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HOT56826.1"
                     /translation="MSTQTQPAAPPPSTAGPEAGVHPDHDRGLEFDVRTLLARRSALG
                     LLSGAGLLALVGCSDASSATSASTASTASATATTSAGGGATASAAAVDSVVPDETAGP
                     YPGDGSNGADVLDDSGIVRQDISSSFGTSTTTAAGVPLAVNLTVLNSADGYAALEGVA
                     VYAWHADAQGRYSMYSQGVENENYLRGVQPTDASGTASFTTIFPGCYDGRWPHIHFEV
                     YRSTSEATSDGQIVKTSQIALPQAACEAVYADGATYVSSADNLARTSLTRDMVFGDDG
                     GIHQLATVTGDTASGYVANLTIA"
BASE COUNT         1667 a         4431 c         4291 g         1600 t
ORIGIN      
        1 gccggatacc ctcacgaccg tgagcctcga ggacaccgac ctgttcggcg cagcagccgc
       61 ggacgcggcg ggagccgacc acccggcatc catcgcaccc ctcgccgtgc gcatgaggcc
      121 gcgcaccctg gcggaggtgc gcgggcaggg tgacgtgctg cgacagggca gcccgctgcg
      181 ccggctcatc gagggcagtg cgggtgcggc cggcccgatc tcggcgatcc tctggggccc
      241 gccgggcacc gggaagacca cgctggcgca cctcgtcgcc accgccgccg accgcgagtt
      301 cgtcgagctg tcggcggtca cggcaggggt caaggacgtg cgggccgtga tgcagcaggc
      361 cacccgggcg cgccagctgt acggccggca gaccgtgctc ttcctcgacg agatccaccg
      421 cttcaccaag gcccagcagg atgccctgct ccccggcgtg gagaaccgcc aggtcgtgct
      481 cgtcgccgcg acgacggaga acccgtcgtt ctcggtgatc gccccgctgc tgtcccgctc
      541 gatgctcgtc acgctcgtgc cgctcaccga tggcgacatc gccgacgtcc tcgcgagtgc
      601 ggtggcggat gagcgtgggt tggccggcgc ctaccgcctc gacgacgacg ccagcgagca
      661 cctcgtgcga ctggccggcg gcgacgctcg gcgggcactg accttcctcg aggcggcggc
      721 gggagtcacc gaggatgccg tggcgccggg cgcgccccgc ccggacgtcc tgccgatcac
      781 cctcgcgcag tgcgagcagg ccgtcgccca cgccgccgtt cgctacgacc gcaccggcga
      841 ccagcactac gacgtcgcct cggccctcat caagtccatg cgcggctccg acgtcgatgc
      901 ggcgctgcac tacctcgcga ggatgctcga ggccggcgag gacccgcgct tcatcgcgcg
      961 gcggatcgtc atctcggcgt ccgaggacgt cgggctcggt gacccgaccg cgctccagac
     1021 cgccgtcgcg gccatgcacg ccgtcgccca gatcgggatg ccggaggcgc ggatcatcct
     1081 cgctcaggcg gtggtccaca acgccatcgc gcccaagtcc aacgcggcct acgtgggcat
     1141 caacgaggcc atcgccgacg tccgggccgg ccgcgggggc ccggtcccgg cgcacctgcg
     1201 gggcagcggc tacgccgggg cggctcgcct cggtcacggc aagggctacg tctacgccca
     1261 cgacgaaccg gatgccgtcg cggcgcagca gtacctgccc gacgacctcg cgggcaccac
     1321 cgactactac cgcccgagcg accgcggctt cgaggcgcgg ctccaggagc gctggacgtg
     1381 gctcaagggc cgcctcggac gctgacgaca cggtccgggg cggtagggtc gcgcccatgg
     1441 aatggacgct cggaggaatt gccgcgatca tcgccgcgct cgcgttcgcg tacctcgtgg
     1501 ttcggctggc cgcgctgctg gggcgtgcga gcacgatcct cgacgagacc gcggcgagcc
     1561 tgcgcacgac gagcgagaac gtgcagccca ccctcaaggg actcaccgac acggtgaccc
     1621 tcaccaacga ccagctcacc cgggtcgaca ccatcacgag ttcggtgtcg acgatgacga
     1681 ccaacgcctc ggcgctgacc tcggtggccg cggccaccgt cgggtcaccg ctgatcaagg
     1741 tggcggcgtt cagctacggc gtgcgcacgg ccgtgtcggg cctgaagaag gcacgctgat
     1801 gaggctgcgc accgtggtgc tcacggctgc ggcggtcggg atcggctacg ccgtgtggcg
     1861 gatgccggcg acccagcggt tcgtggccac ggtcaaggag gcctcggccc ggcgcgaggc
     1921 cgagcttcgc gaggccatcg aggtggccgt gctcaccgac gcgacgaccc gtgccccgcg
     1981 ccacgccagc ggtgtcagca cccccggact cgacgtcacc gcgggctggg acgacgaccg
     2041 ccggccccgc acccactccg ccgccgcgct gggggccgat gacccggggc gctcgctgac
     2101 cccggaagag gcgaaggcgc tgttgctcga ccccacgggc gagcgagctc agtaggggcc
     2161 gccagccccc accgccgcgc ggggcgcacc gatcacgacc ccgacccacc gaacgaccga
     2221 acgccgccga gagcatcccg gcatacgatg acgcgtgccc gcaccccggt ggcaccgatg
     2281 actcccaagg actgagaaga catggaaacc gctgagatcc ggcgccgctg gctggccttc
     2341 ttcgagaaga acggccacac cgtcgtcccc tccgcgccgc tcatccacga cgacccgaac
     2401 ctgctgttcg tcaacgccgg gatggtgccg ttcaagccct acttcctcgg ccaggagacc
     2461 ccgctgtggg accgtgcgac cagcgtgcag aagtgcgtgc gcaccgggga catcgaggag
     2521 gtcggcaaga cctcacggca cgggacgttc ttccagatga acggcaactt ctccttcggt
     2581 gactacttca aggaaggtgc catcaccctc gcgtgggagc tggtcaccac ctcgcaggcc
     2641 gagggtggtt acggcctgga tgccgacaag atctggccga ccgtctacga ggacgacgac
     2701 gaggccttcg ccatctggcg tgacacgatc ggtatccccg tcgagcggat cacccggcgc
     2761 ggcaagctcg acaactactg gcacatgggc gtccccggcc ccggcggccc gtgcagcgag
     2821 atctacctcg accgtggccc ggagtacggg ctcgagggtg gcccggctgt cgacgaggac
     2881 cgctacctcg agttctggaa cctcgtgttc atgcaggagg agctctcggc ggtccgcgcc
     2941 aaggacgact tcgacatctc cggcccgctg cccaagcgca acatcgacac cggcatgggc
     3001 ctggagcgca tggccaccct gctccaggga gtcgacaacc tctacgagat cgacgaggtc
     3061 tatccggtca tcgcccgcgc ggccgagatg accggcaagc gctacggcga gcactcgggc
     3121 cagtccgcgg ggtccagcca ccccgacgac gtccggctgc gggtcgtcgc cgaccacgtg
     3181 cgctcggcgc tcatgctcat cggtgacggg gtcacccccg gcaacgaggg ccgtggctac
     3241 gtcctgcgcc ggatgctgcg ccgctcggtg cgctcgatgc gcctgctcgg ctacgaggac
     3301 ccgtgcctgc cggagctgct gccggtctcc ctggagcgga tgcagaactc ctaccccgag
     3361 ctggccacgg gcttcgaccg gatcgcccag atcgcctacg ccgaggagga cgccttccgc
     3421 cgcaccctgg ccgccggcac gacgatcctc gacaccgccg tcgcccgcac caaggatgcc
     3481 gggggctcca cgctcgcggg ggagcaggcc ttcgcgctgc acgacaccta cggcttcccc
     3541 atcgacctca ccctggagat ggctgccgag cagggcctgg aggtggaccg ggacgggttc
     3601 acccgcctga tgcgcgagca gcgtgaacgc gcgcgggccg acgccaaggg caagaaggcc
     3661 gggcacgcca acaccgaggt ctggcgtgac ctgcgggcca gcggcgcgac cgacttccgc
     3721 gcctacgagg agctgacgag tgaggccacc gtcgtcggcc tcgtcgtcga cggcgaggcc
     3781 gtgcaggaga tcgcccccgg ccagcagggc caggtcgtgc tcgaccgcac ccccttctac
     3841 gccgagtccg gtggtcagat cgccgacgag ggcatcatca cggccgacgg tgtgcacctg
     3901 aaggtcatcg acgtccagcg gccggtcaag ggcctcatcg cccacaccgt cgaggtcgtg
     3961 accggtggcc tgcagaccgg cacgagcgtg ctggcccagg tcgaccccga gtggcggctc
     4021 tcggcctgcc aggcacactc cggcacgcac gtcgtgcacg ctgccctgcg ccaggtgctc
     4081 ggcccctcgg ccctgcagtc cgggtcgtac aacaagcccg gctacctgcg actcgacttc
     4141 gcctgggggc aggcgctgtc cgcggccacc cgcagcgaga tcgaggaggt cgcgaacctg
     4201 gcggtccgtc gtgacctgcc ggtgtcggcc acgtacatgc cgctgcccaa ggcccgcgag
     4261 atcggcgccc tggcgctctt cggcgagacc tacggcgagg acgtgcgggt cgtcgagatc
     4321 ggcggcgagt ggagccgtga gctgtgtggc ggtacccacg tgcagcactc cagccaggtc
     4381 ggtgccctca ccctgaccgg tgagtcctcc gtggggtccg gtgtgcggcg cgtcgaggcc
     4441 ttcgtcggca tggaggcgct gcgcttcctg gcacgcgagc gagcgatcgt cgccgagctg
     4501 agcggcctcg tcggtgcgcg ccccgacgag ctcaccgacc gcatcggggc catggtcacc
     4561 cggctcaagg acgccgagcg cgagctcgag cgggtgcgca aggaggccgt gacggcggcc
     4621 gcgggcaacc tcaccgagca ggcccgcgac gtcaacgggg tcaccttcct cggccacgac
     4681 gccggggatg ccggcgctga cgacgtgcgc acgatggtca tggacctgcg gggtcgcctc
     4741 ggcaaccaga gccccgtcgt cgtcgccatc acgggtgcgg ccaaggggcg ccccgtcgtc
     4801 gtcgtggcga ccaacgaggc ggcccgggcc cgcggcatcc gggccggtga gctcgtccgg
     4861 gtggcggcca cgaccctcgg cggcggtggt ggcggcaagg acgacatcgc ccagggcggt
     4921 gggcaggacg tcgccaaggt cggcgaggcc ctgtccgcgg tggagtggcg cgtcggggag
     4981 ctcgccggct gatgccggag ccgtccggga cctggatcgg tgtcgacgtc ggcaccgtgc
     5041 gggtcggcgt cgcccgcagc gacccgcacg gcatcctcgc caccccggtg gcgacgctgg
     5101 cccgggaccc ggatgccgcc gtgtcaccgc aggacccgga cgtcaccgcg atcgccggcc
     5161 tcgtcaccga actcggcgcg gtcggcgtga tcgtcggcct gccccgctcg atgtccggtg
     5221 tcgagggtcc gagcgcagcg aaggcgcgcc agtatgcttc cgtcgttgcg gcgcgcatcg
     5281 cgccggtgtg ggtgcgtctc atcgatgagc gcctgaccag tgtcgatgcc caccgcacgc
     5341 tgcgtgacag cggtgtctcc ggtcggcgac agcgcgcggt ggtcgaccag gccgctgcgg
     5401 tgctgatcct ccagaacgcc ctggacaccc agcgcaccac cggatcccca cccggggaac
     5461 aggtccggat cggacggcgc aagccacgga cgaaggatgt caacccgtga cgcagggcga
     5521 cgtctcccac acgatcttcg gtgagcccga gccgccgccc acgcggcgtc gggggcacca
     5581 ccgccacgcg aagcgcaaca accgccgctg gctggtgctg ctcatcgcgc tcgccctgct
     5641 cggcggtgcc gcgtgggctg ccgtgagcgt gatcaagccg gtgctgtcct cggtgttcaa
     5701 cggtggcggt tcggagaccg ctgacttccc cggccccggg gagggtgcgg tcgagatcgt
     5761 cgtcaccccg ggggagaccg gcgaggacat cgcgacgacg ctgcgggacg ccggcgtggt
     5821 gaagaccagg ggggcgtaca tcgacgtcgc tcggtccgac cccgagcggg ccgcggccat
     5881 ccagcccggc aggtacaacc tgctcaaggg gatgacggcc gccgaggcgt tcgcggtcct
     5941 cggtgacccg gccaaccgga tcacctccgg cacgacggtg cgcgaggggc tgtgggccac
     6001 cgagaccttc gaggtgctgt cgaagtcgac cggcatcccg gtcaaggagt acgccgctgc
     6061 ggccaaggac gcgcgggcga tcggcctgcc cgccgaggcg gagggcaacg tcgagggctg
     6121 gctggccccc tcgacctacg agttccccga gaactccacg gccgccgaac agctcgcggc
     6181 catggtcgcc cagaccgtga aggtgctcga caccgcgggg gtggccccga aggaccgcga
     6241 gaacgtgctc atcctcgcct cgctcgtcga ggccgaggcc aagctcgacg aggaccgacc
     6301 caagatcgcc cgggtgttcc tcaaccggct ggaaaccgac ggggcgccga cctacggtct
     6361 gctccagtcg gacgcggcgg tgtcctacgg ggctcagcgc aggtcgttgt tccccagcga
     6421 ggccgagctc aacgacgcca gcaacccgta caacacccga ctgatccccg ggctgccgcc
     6481 gggcccgatc agcaaccccg gcgcggcgtc gatcaaggcc gccgcgaacc ccgcggacgg
     6541 cccgtggttc ttcttcgtcg ccgtcaaccc catcaccggt gagaccaagt acggggtcac
     6601 cctggacgac cacaacaaga acgtgcgtga actgaccgcg tactgcaagg agaagcccaa
     6661 ggactgcggg ctgtgagggc tgccgtcctc ggctcaccga tcgctcactc gctgtcgccg
     6721 gtgatccacc gggccgggta cctggccgcc gggctcaccg ggtgggagta caccgcccac
     6781 gaggtcgacg ccgacgcact tcccgggttc gtcggcggcc tggacacctc ctggcgtggc
     6841 ctgtccctga cgatgccgct caaggaggtc gcgggtgcgc tggccacgac ggtggatgcc
     6901 gtggccaccc ggtccggggc cgtcaacacc ttggtgcggc gcgccgacgc agggtgggac
     6961 gccacgaaca ccgatgtcgt cggggtcatc gaggcgctgc ggccgcacct gcccgaccgg
     7021 gtgacccgag ccctggtgct cgggtccggc gccaccgccc gctcggccgt gctcgccctg
     7081 gccgagctcg gcatcacgac cctcaccgtg cgggcccgcg acacggccaa ggcggccgac
     7141 ctgctgagct gggccctcgg gctggacatc gggatccgca acggctcggt ggccgacctc
     7201 gggccctggg tgagcacccg tgacgacgtc gtggtctcca ccctgcccgc gacggctgcc
     7261 gaggcggtcg ccgccacggt cccggcggcg caccacggca tcctgctcga cgtcggctac
     7321 gacggctggc ccacgccccc ggcgcgggcc gcggccgccg ccgggatgac ggtggtgtcg
     7381 ggcctggaca tgctcgtgca ccaggcggcc gagcagttcc gtctcttcac cgggcacgac
     7441 gccccggtgg ccgagatggc cgccgcaggt cgggcggccc tggcccggcg atgactccgt
     7501 ggtgggtggt cgtggccgct gccctggtca tgggggtggt cggccacctc accgggcagc
     7561 agctcgcgac gggcggctac cggatccccg aggacgaggc cgagcacgcg gtggggcgca
     7621 actggtggcc cgggctgctg tgcggtgcgc tgtcggcggt ggcggcctgg gccgtcggcg
     7681 acctcgcggg gtgggcagcc ctgccggcct acctgctctt cgcgtggctg agcgtcggcc
     7741 tcatctggat cgacctcgac gtgcaccgcc ttccggtggg tctcgtcgtc cccacgggct
     7801 ggggcatcct cgggctcctc ggcgtcgcgt ccctcgcgtc cggggaccgg cgctggctcg
     7861 gcgccgtcgt cgggttcgtc gtcatggggg tggcctacct cgtgctggcg gtgctgcccg
     7921 gtggtggtgt cggcggggga gacgtgcggc tggcgcccct gatcggggcg ctgctcgggt
     7981 ggctcggcgc agcccacctg gtgatcgggc tggccgccgg gttcctgatc ggcggggtgg
     8041 ccgctgcggc gctgctcgtt ctcggccggg cagggctgaa gtcgtcgatc gcgtacggcc
     8101 cggcgatgtg cctcggggcg tgggtgggca tcgccgcgac atcccggatc ctgaccgtgc
     8161 tgtcgggcgg ctgagcgtcg cgtgacagga ttctggccat gctgcgctgg ttgaccgccg
     8221 gggagtcaca cggccccgcc ctcgtcgcga cgatcgacgg gttgcccgcc ggagtcgtcg
     8281 tctccagcga cgacctcgcc cgggcgctgg cccgccgccg cctcggccac ggccgcgggg
     8341 cccggatgtc cttcgagcgt gacgacgtgg agttcctcgg cggggtgcgc cacggcgtga
     8401 ccctcggctc gcccatcgcg atccgggtgg gcaacagcga gtggcccaag tggcagaccg
     8461 tcatgagccc cgaccccgtc gcagccgacg acctcgccgc ggcgaacgac atcaacgccg
     8521 agaaggagat cgcccgcaac cggccgctca cccggccccg ccccggccac gcggacctcg
     8581 tcggcatgca gaagtacggc ttcgacgacg cgcgccccgt gctcgagcgg gcgtcggccc
     8641 gcgagacggc cgcccgggtg gcgctcggtg aggtggcggc ccgcttcctc gaccaggcgt
     8701 acggcatccg cctcgtgtcc cacacggtcg ccatcggtgc cgccggcgtc gccgaggacg
     8761 caccgttgcc gaccgctgac gacgtcgcgg cgctcgacgc cgacccggtc cgggccttcg
     8821 acgccgctgg ctccgcggcg atggtcgccg aggtcgacgc cgcgcacaag gacggcgaca
     8881 ccctcggggg agtggtcgag gtcgtcgtca ccgggctgcc gccgggcctg ggctcgcacg
     8941 tgcactggga ccggcgcctc gacgcgcggc tcgcgggggc cctcatgggc atccaggcca
     9001 tcaagggcgt cgaggtcggc gacggcttcc gcaccgcgac ccggcgcggc tccgtggccc
     9061 acgacgagat ggagcgaggt cccgacgggg tcatccgccg gcgcaccggg cgggccggcg
     9121 gcaccgaggg cggcatgtcg gtcggcgggt tgctgcgggt gcgcgcggcc atgaagccca
     9181 tctcgacggt gccccgggcc ctgcagaccg tcgacgtcgc ctccgacgag cccgcacgcg
     9241 ccatccacca gcggtccgac gtctgcgcgg tgccggcggc cggggtcgtc gccgaggcga
     9301 tggtcgccct cgtcctggcc gaggcctgcc tggagaagtt cggcggggac tccgtcggtg
     9361 agacggcacg caaccacgcg tcgtacctgg ccgcgatccc ggcctcgatg cgcacctggg
     9421 acgacgaatg agcccgcggg tggtgctcat cgggccaccc gggtcgggca agagcaccgt
     9481 cggggcggcg ctggccgcac ggctcgggct gccgctgcac gacaccgacg ccgcgatcga
     9541 ggcggctgcg ggccgctcga tctcggacat cttcatcgag gacggcgagc ccaccttccg
     9601 tgagctcgag cgggccgagg tggcccgtgc ggtcgagcag gaggacggtg tgctggcgct
     9661 cggcggcggt gcccccgtgg accccctgac cgagcaggtg ctcgccgggc aggtcgtcgt
     9721 gttcctcgac gtcggcatcg ccgatgcctc caagcgggtg ggcttcgacc agagccgccc
     9781 cctgctcgcg gtcaaccctc gggcgtcgtg ggtgcgcctg atgaacgagc gccggcccgt
     9841 ctacgagcgg gtcgcgaccc accgcgtcga caccgcagga cgtaccccgg acgccgtcgc
     9901 cgaggagatc gccaccctgc tgggggcgtc gtgacggcgc cggcgaccgt catcccggtc
     9961 ggaacctcct atgacgtcgt cgtcggccac ggggtgtccg accgcgtcac cgacctgctc
    10021 tccggggacg tccggcaggt tctcgtcgtg cggccggagt cgctggcccg tctggcagcc
    10081 ccggtggtcc accggttgcg cgaggccggg ctgaccgtgc acgagactgc cgtcccggac
    10141 aacgaggtgg ccaagaccgc cgaggtggcg gcgggcctgt gggccacgct gggtcagcac
    10201 gcgttcaccc gctcggatgc cgtggtggcg gtgggtgggg gcaccgtcac cgacctcgcc
    10261 ggtttcgtcg cggccacgtg gctgcgcggg gtcgccgtgg tgcacgtccc gacgaccctg
    10321 ctcgcgatgg tcgacgcagc cgtcggcggg aagaccggga tcaacacggc cgagggcaag
    10381 aacctcgtcg ggtccttcca cgagccggcc ggcgtgctgt gcgacctcga tgccctcgcc
    10441 accctgcccc gacctgacct cgtcgccggc ctggccgagg tcgtcaaggc cgggttcatc
    10501 gccgacccgc gcatcctcga gctcgtcgag ggcgaccccg acggggcgac ccgcgcggac
    10561 gggcccgcga tgcgtgagct cgtcgagcgg gccatcgcga tgaaggccga ggtcgtcgcc
    10621 gccgacctca aggagtcgtg gctgcgcgag atcctcaact acggacacac cctggggcat
    10681 gccgtcgagc aggtcgaggg ctaccgccgt cggcacggtg aggcggtggc gatcggcatg
    10741 acctatgccg cgcacctggc ccaccgggcc gggcacctca cggcggatgc cgtggcgcgc
    10801 caccgcgcga tcctcacctc ggtgggcctg cccaccacct acgaggcggg tcactgggac
    10861 gccctggcga cggcgatgca gcgcgacaag aagaccaggg gttcgttgct gcgcttcatc
    10921 gtcctcgagg acatcggggc gcccacgcgt ctcgtcggcc cggacgaggc gctgctgcgc
    10981 gccgcctacg acgacctctg ccgctgagcg gcacaccgcg ggtgcgcggg cgcacaggtg
    11041 gcccacagca ggtgcacgga cgggtcatgg cgggctccag cagggttctc gccatgagca
    11101 cccagacgca gccagctgcc ccaccgccct cgaccgccgg ccccgaggcc ggcgtccacc
    11161 cagaccacga ccgcggactc gagttcgacg tgcgcaccct gctggcccgg cgctccgcgc
    11221 tcggcctgct ctccggtgcg ggcctgctcg ccctcgtcgg ctgctccgac gcgtcatccg
    11281 caacgtccgc atccacggca tccacggcat ccgccaccgc cacgacgagc gcgggtggcg
    11341 gcgcgacggc atccgccgcc gccgtcgact ccgtcgtgcc cgacgagacg gcaggcccct
    11401 acccgggtga cggcagcaac ggcgccgacg tgctcgacga ctccgggatc gtccggcagg
    11461 acatctcctc gtcgttcggg acctcgacga cgactgccgc cggcgtcccg ctcgccgtga
    11521 acctcaccgt cctcaactca gccgacgggt acgcagcgct cgagggcgtc gccgtctacg
    11581 cgtggcacgc cgacgcccag ggccgctact cgatgtactc ccagggagtg gagaacgaga
    11641 actacctgcg cggcgtgcag cccaccgacg cgagcggcac ggccagcttc accacgatct
    11701 tccccggctg ctacgacggg cggtggccgc acatccactt cgaggtctac cgcagcacct
    11761 ccgaggcgac gagcgacggt cagatcgtca agaccagcca gatcgcactg ccacaggcgg
    11821 cgtgcgaggc cgtctacgcg gacggtgcga cgtacgtgtc gagcgccgac aacctcgccc
    11881 gcacgtcact gacgcgagac atggtcttcg gtgacgacgg cggcatccac cagctggcca
    11941 ccgtgacggg cgacaccgcg tccggctacg tcgcgaacct cacgatcgc
//
LOCUS       DAOSOC010000067        11824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5377_length_11824_cov_10.258731, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000067 DAOSOC010000000
VERSION     DAOSOC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5377_length_11824_cov_10.258731"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..124)
                     /locus_tag="PL014_06765"
     tRNA            complement(52..124)
                     /locus_tag="PL014_06765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Ala,seq:cgc)
     gene            193..1452
                     /locus_tag="PL014_06770"
     CDS             193..1452
                     /locus_tag="PL014_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT56827.1"
                     /translation="MRFRRPTPTEVGATPGDPRPANPSASTPVLLVPAAVDADWSVPS
                     GVRKASEWAWRGLVIAAALVAVLYLISLLSEIVIPLLMALLLAALLQPAFQWLARFMP
                     RGAAAGLTVVGTLVFVFGLLSFVGSQLTSQLDDMTTKVTDGIDQIRRWLNSTFGITDT
                     QLEGYIDRAREWLSSQNLADTATSAGLTATHILAGFFLGLFALFFFLYDGATIWRWVT
                     QLFPRGARARVRSSGDIAWVQLSSFTRATLLVAASDAIGIGVGAAILGVPFASGITLL
                     VFFGAFIPVIGAAVSGTVAVMLALVALGPVQALIMLGIVIGVQQLESHVLQPFLVGRV
                     MRLHPLAVLLAIAAGIVLAGIVGGLIAVPAVAVLNAVGHHLLDEADGESEHVGAAAAD
                     EAHVLTSGEAERAEEAVDATDSPGQGE"
     gene            complement(1517..2488)
                     /locus_tag="PL014_06775"
     CDS             complement(1517..2488)
                     /locus_tag="PL014_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56828.1"
                     /translation="MQSPSSLIFVLLLGVWAAYFVNYLVRRRDHLSTARSVDQFSSAM
                     RVLDRRDSAAPSTPVAAEPAGPRAYAVHPARSASAGSPVVTERVAPGAASRPVRSVRP
                     QISPSRRVRGITLLAVAAALLISLPLALFSVLAAWAPLVPLAALGASLAWVRSGVRAQ
                     ASLTRAHRRRQAELARLRTTAPVTVPAARRGATATRPAPTATTATAADVATGATASAP
                     VEPAVPAVVASVEAPAVEPAAVVAPVAAAPLATQPAAEVMVPILDEDDMPLTWDPVPV
                     PRPTYTMKAMAPRAEVEPAEVTPTPAPVERQSESESPVYDGRRAAGA"
     gene            complement(2572..3300)
                     /locus_tag="PL014_06780"
     CDS             complement(2572..3300)
                     /locus_tag="PL014_06780"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT56829.1"
                     /translation="MWDAVRRGAARLAGAGSGSAGGAGGEGSAQRRDGDSGAGIEWPL
                     RLTGRTPLGTDIVLRPLTVADEAVFHSVRRANAAWLGPWDATSPDPAAPLRGFAELVA
                     HYDAEARAGRSMPLVVEAEGRLVGQLSIGGIMLGSFRSCLVGYWVSRSVAGRMVIPTA
                     LAVAGDHAIDALGLHRIEVNIRPDNTASLAVVRKLGFRDEGLRLNYLHIDGAWRDHRS
                     FALTTEDLDGETLRDRLTRLTSEA"
     gene            complement(3300..4346)
                     /gene="moaCB"
                     /locus_tag="PL014_06785"
     CDS             complement(3300..4346)
                     /gene="moaCB"
                     /locus_tag="PL014_06785"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HOT56830.1"
                     /translation="MTDSTSKARTTSGLTHVRADGTAHMVDISAKEVTARRAAASGMV
                     LLSAAAVAALRGGAVPKGDALGVARVAGIQAVKRTPDLIPLAHPVVVHAVEVDLDVVD
                     EGVRISAVVRTADRTGIEMEALTAVSVAALALVDMVKAVDKHARITDIRVTAKSGGRS
                     GDWKEPDVLDAVTTGSRSARHGPRPPTPSLAGARAVVITCSTRAAAGVYPDRGGAVLV
                     ETLRAWGAEVPDALVVPDGPSVGRALASALATSPDLLVTTGGTGLTPTDGTPEATRPL
                     LDYEVPGLAEAIRARGVAKGVPTAVLSRGLTGVAGHTLVINLPGSSGGVRDALGVLAE
                     VLPHTLSQVRGGDH"
     gene            complement(4339..5559)
                     /locus_tag="PL014_06790"
     CDS             complement(4339..5559)
                     /locus_tag="PL014_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOT56831.1"
                     /translation="MRTLEEHRAAVLALVSPLPATRVPVAAALGLVLAQDVTALVDLP
                     GFDNSAMDGYAVRASELAGASSENPVVLPVSGDIAAGDTTRHVLAAGHTMRIMTGAPL
                     PEGCDAVVPVELTDGGSGQVALFLEPDAGRHVRRRGEDITAGSVVLRAGTRLTPGHLA
                     IAAAANVAELPVHPRPRVTVVSTGDELVAPGSTLAHGQIVDSNHVMLRALVEAAGAEV
                     AAGVHLRDEVEAVRALVDAPPGVPDLVITSGGVSMGVYDTVKEVLSADGGVEFVKVAM
                     RPGMPQGSGLLGAHRTPVITLPGNPVSSFASFHVFVLPVLRRLAGLDPDDDGSFEAEA
                     SVGWPTVAGKVELTRVTERAGRISPSGGQGSHVLGALAAATALAVVPADVEHVAAGSV
                     VRCLPLLGQDRPRD"
     gene            complement(5559..6944)
                     /locus_tag="PL014_06795"
     CDS             complement(5559..6944)
                     /locus_tag="PL014_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOT56832.1"
                     /translation="MTTQGLEAATEKMRSAGVPGVAIDVFSRFHELLRSQVTGLIPED
                     TISPLEQVTRLDDVVAGSAGAIREGLARTVIIKLNGGLGTGMGIRGPKSALPVRDGLT
                     FLDIIARQVLALRRRHGVDTPLLLMNSFRTREQSLEILERYDDLPVDELPLDFLQSME
                     PKLRADDLSPVEWPDDPELEWCPPGHGDLYVALYTSGLLDSLRQRGYRYAFLSNADNL
                     GATCEGAVPAWMAANGIPYVTEVCPRTHNDRKGGHLAVRRSDGRLVLRDSAAVAPGED
                     HYFQDTERHHWFHANNLWIDLDVLARHLGERGGILGLPIIVNRKTVDPTRPQSTPVIQ
                     IESSMGAAVELFEGSRALAVDRSRFRPVKTTNELLLIRSDLYRLTPECEVESTITHPE
                     PFIDLGPAYRHVEGFEQRFPRGVPSIRDCTSLRVEADATFGADVVCTGDVRVTGGVRA
                     IPDGAHLEGDV"
     gene            6969..7565
                     /locus_tag="PL014_06800"
     CDS             6969..7565
                     /locus_tag="PL014_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOT56833.1"
                     /translation="MARDPVKTARRASLRARRRDLAAARDTARDDHDLAGHVGALVDT
                     LGLGPGSVVTSYASIPGEPPTARLNAMLAERGIRVLLPITLPDLDLDWHDAADPGEQP
                     LGRESVAAVDLILAPGLSVDHSGTRMGQGGGCYDRALTRCDPRPPTVVLLHPGELVAV
                     DDAPLPREPHDRGVDAVLTAEGLVDLGLSPWSSPRGAG"
     gene            complement(7531..10242)
                     /locus_tag="PL014_06805"
     CDS             complement(7531..10242)
                     /locus_tag="PL014_06805"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOT56834.1"
                     /translation="MSRVVVVRRVLLGLIAVLVVAALVAGVVVTTTVRASLPKTTGTA
                     DLPGLGADVSVLRDDSGIPHLYGDSITDLARAQGYVHAQERFFEMDLRRHVTAGRLAE
                     LVGADGLESDKVIRTMGWRRIAEEELPTLKPQTRQVLQAYAEGVNTYLRGRSPGDVAV
                     EYTLLGLRLPDVTIEEWTPVDSISWLKAMAWDLRGNYEDELARARLSGRLSPAQIAQI
                     YPPYDSATRPPILSDQEWSPTDAGRPVSSSVPSALTEATASAPTTSDAVASPRAAAAP
                     AAAAASADDPVVSASAQQAYAVVQDALDAVPQLLGRGEGIGSNSWVVAGSRTSTGKPL
                     LANDPHLALGQPGVWIQNSLRCRTVSSTCPLDVSGFSFAGVPGVIIGHNADIAWGMTN
                     LKPDVTDFYLERVVGDTYLRDGDWAPITYREEVIKIRGGADQRITVRSTVHGPVMSDV
                     VEGVNDAGGRAPTQQEGDENETYAVSLAWTGLIPSRTADAILDLGLATNFQEFREAAR
                     SFAVPSQNLVYADTEGNIGYQAPGQVPTRRSAIPRAAPGFWPAPGWDPEYDWTGYVAF
                     EDMPWALNPADGVIVAANQAVVAGETPFLTTDWDRGWRSTRIAERLSGLNSISPVEMA
                     AVQLDDQDPFAKVLVPALLGVTLEASEGDQISQELLDFTREARELLRGWDYTTPAGDS
                     DSAASAAYYNAVWRNLVKLLFDDELPVGLKSDGGARWRAAVTDLLDDPDSAWWDNKLT
                     PNITESRDEILRQSLVQARLELTRELGKDPGQWQWGKLHRLTLEHPVLGGESMPGAVR
                     WLVNEGPFDVPGGSAIVNANGWNASLGYEVTAGPSMRMVVDLGNLDGSTWINSTGVSG
                     HPADARYSDQVEDWVSGKQRPWPFSEDAVRAQDPDVLTLRPEGSTTG"
     gene            10288..11823
                     /locus_tag="PL014_06810"
     CDS             10288..11823
                     /locus_tag="PL014_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOT56835.1"
                     /translation="MRQTWTTPFADGGATTGFDLHDLTLVLLLGSIVLVVSVAAVRLS
                     VRSGLPSLLIYLGIGVLLGEGGFGINYDSQQLTQVLGYAALVLILVEGGLTTRWSGIR
                     RSVAPAAVLATVGVGVSIVVVGVAAKLLLGWSWEIAFLVAAVLASTDAAAVFSVLRRV
                     PLPRQISGMLEAESGFNDAPVVLIVTALAASLAPGATPEPWWQVGVIAVLELAGGSLI
                     GLAIGYGGARLLRVVATTSSALFAIGVIAVAVLAYAAADVVHASGFIACYLAALVLGN
                     LRLPHRPAVTGFATAIGWLAQIGLFVLLGLLASPSGFDDVLVPALVVGTALLLLGRPL
                     SVIASCAPFGMSLRTQAFLSWAGLRGAVPVVLATVPVTVGAVGVEWIFDLVFVLVVVF
                     TIIQAPTLPWVARQLGLGEGHHQVDLAVDATPLEELGADLLEIDVGPGSKIAGVRVFE
                     LRLPVGANVSLVVRGGTSFVPRESDVLRHGDQVLVVTPQAVRDDTEKRLQAVSRGGRL
                     AGW"
BASE COUNT         1565 a         4247 c         4333 g         1679 t
ORIGIN      
        1 cgcaaagcag aaggacccag caaaaagcat gctgggtcct tctgctgtcg gtggagctga
       61 ggggattcga acccctggcc ttctcattgc gaacgagacg cgctaccaac tgcgccacag
      121 ccccgagagc gacgcaacga taacaccgct gactcgggac gtcgaaaccg ctgggcgacg
      181 tagcatcgcg ccatgcgctt tcgccgtccc actccgaccg aggtcggggc cacccccggc
      241 gacccccgcc cagccaaccc atcggcatcc acccctgtac tcctggtccc cgcagccgtc
      301 gacgccgact ggtcggtgcc gtcaggggtg cgcaaggcca gtgagtgggc gtggcgcggt
      361 ctggtcatcg ccgccgccct cgtggccgtg ctctacctga tcagcctgct gtccgagatc
      421 gtcatccccc tgctgatggc cctgctcctc gcggccctgc tgcagccggc cttccagtgg
      481 ctggcccgct tcatgccccg tggggccgcc gcgggcctga cggtggtcgg caccctcgtg
      541 ttcgtgttcg gcctgctgtc cttcgtcggg tcgcagctga ccagccagct cgacgacatg
      601 acgaccaagg tcaccgacgg catcgaccag atccgccgct ggctcaacag cacgttcggc
      661 atcaccgaca cccagctcga gggctacatc gacagggccc gcgagtggct gagctcgcag
      721 aacctcgccg acacggcgac ctccgccggg ctcaccgcca cgcacatcct cgccggcttc
      781 ttcctcgggc tgttcgccct gttcttcttc ctctacgacg gcgccaccat ctggcgctgg
      841 gtgacccagc tcttccctcg cggcgcccgc gccagggtgc gctcctccgg cgacatcgcg
      901 tgggtccagc tgtcctcctt cacgcgcgcc accctgctgg tcgccgcgtc ggatgccatc
      961 ggcatcggtg tcggcgctgc gatcctcggc gtgccgttcg cctccggtat cacccttctc
     1021 gtgttcttcg gcgccttcat cccggtgatc ggtgcggccg tctccggcac ggtggccgtg
     1081 atgctcgcgc tcgtggccct cgggccagtg caggcgctga tcatgctcgg catcgtcatc
     1141 ggggtgcagc agctcgagag ccacgtgctg cagcccttcc tcgtcggtcg ggtgatgcgc
     1201 ctgcaccccc tcgcggtcct gctcgccatc gccgctggca tcgtgctcgc cggcatcgtc
     1261 ggtggcctca tcgccgtgcc tgccgtcgcc gtcctcaacg ccgtgggcca ccacctgctc
     1321 gacgaagccg acggcgagtc cgagcacgtc ggtgccgctg cggccgacga ggcccacgtg
     1381 ctcaccagcg gagaggcgga gcgtgccgag gaggccgtcg acgcgaccga ctcccccggg
     1441 cagggcgagt agctccccag ccccaacccg acgagccggt ccgtcaggga ctcccagccc
     1501 ctacccggcg agtcggtcag gcgccggctg cgcggcggcc gtcgtagacc gggctctcgg
     1561 actccgactg gcgctcgacg ggagcggggg tcggggtgac ctcggccggc tcgacctcgg
     1621 cccgcggcgc catggccttc atcgtgtagg tggggcgcgg caccggcacg gggtcccagg
     1681 tcagcggcat gtcgtcctcg tcgaggatcg gcaccatgac ctcggcggca ggctgagtgg
     1741 ccagcggggc ggcggcgaca ggggccacga cggcggccgg ctcgacggcc ggggcctcga
     1801 ccgacgcgac gacggctggc actgcgggct cgacgggcgc ggatgccgtg gcaccggtcg
     1861 cgacgtcggc tgcggtcgcc gtggtggctg tcggtgccgg gcgggtggcg gttgcgccgc
     1921 ggcgggctgc cgggacggtg accggcgcgg tggtgcgcag tcgggcgagc tcggcctggc
     1981 gacggcggtg ggcccgggtc agcgacgcct gcgcacggac accggagcgc acccacgcca
     2041 aggacgcccc gagggcggcg agcgggacga gcggggccca cgcggcgagg accgagaaca
     2101 gggcgagcgg caggctgatg agcaaggctg cggccacggc cagcagggtg atgccacgga
     2161 cgcggcggct cgggctgatc tgcgggcgca ccgagcgcac cggacgggac gccgccccgg
     2221 gagccacccg ctcggtgacg acgggcgagc cggcggaggc agatcgggcg gggtgcactg
     2281 cgtacgcgcg aggtccggcc ggctcggccg cgaccggggt cgagggcgca gcggagtcac
     2341 gccggtcgag cacccgcatg gccgaactga actggtcgac cgagcgggcg gtcgacaggt
     2401 ggtcgcggcg gcggaccagg tagttcacga aataggcagc ccacaccccg aggaggagga
     2461 cgaagatcag gctcgacggg gactgcacga ctagaacggt aggcgtgctc ccccactcga
     2521 cgccggaccc ctgtcggtgt gtcgcacaag tgactgctgt gacttctggg atcaggcctc
     2581 ggaggtcagc cgggtgagcc ggtccctgag cgtctcaccg tcgaggtcct cggtcgtcag
     2641 cgcgaacgaa cggtggtctc gccatgcccc gtcgatgtgc aggtagttca gccgcagccc
     2701 ctcgtcccga aagcccagct tgcgcacgac ggccaggctc gcggtgttat cggggcggat
     2761 gttgacctcg atccgatgca gccccagcgc gtcgatcgcg tggtcgccgg cgaccgcgag
     2821 cgccgtgggg atgaccatcc gcccggcgac cgagcggctc acccagtacc cgacgaggca
     2881 ggagcggaac gacccgagca tgatgccgcc gatcgacaac tgcccgacga ggcgaccttc
     2941 cgcctcgacg acgagcggca tcgacctgcc ggcgcgggcc tccgcgtcgt agtgcgccac
     3001 cagctcggcg aacccgcgca ggggcgccgc cgggtccggc gaggtggcat cccagggccc
     3061 gagccaggcg gcgttggccc ggcgcaccga gtggaacacc gcttcgtccg cgacggtcag
     3121 cggccgcagc acgatgtcgg tgccgagcgg cgtgcgcccg gtcagccgca acggccactc
     3181 gatgccggcg ccggagtcac cgtcccgacg ctgcgccgac ccttcaccac cggcgccgcc
     3241 ggcggacccg gaccccgcac cggcgagccg agcagccccc cggcgaacgg catcccacat
     3301 cagtggtcgc cgccgcgcac ctggctcagc gtgtgcggca gcacctccgc gagcacaccc
     3361 agggcgtcgc ggaccccgcc gctggagccg ggcaggttga tgacgagcgt gtgcccggcg
     3421 acacccgtca gaccccgtga gagcaccgca gtcggcaccc ccttggccac cccccgggcc
     3481 cggatcgcct cggccagccc cgggacctcg tagtcgagca ggggccgcgt cgcctcgggg
     3541 gtgccgtcgg tgggggtcaa cccggtgccg ccggtggtga cgagcaggtc gggcgaggtg
     3601 gcgagggccg aggccagcgc gcgccccacc gacgggccgt cgggcacgac gagcgcgtcg
     3661 ggcacctcgg caccccacgc gcgcagggtc tcgacgagca cggcaccacc gcggtcgggg
     3721 tagaccccgg cggcggcgcg ggtcgaacac gtgatgacca ccgcgcgcgc cccggcgagc
     3781 gacggggtcg gcgggcgcgg cccgtggcgc gccgacctcg aaccggtcgt gacggcatcc
     3841 aggacgtcgg gctccttcca gtcgccggac cgtccgccgg acttcgccgt gacccggatg
     3901 tcggtgatgc gggcgtgctt gtcgaccgcc ttgaccatgt cgacgagcgc gagcgccgcg
     3961 acgctgaccg cggtcagggc ctccatctcg atacccgtgc ggtcggcggt gcggacgacg
     4021 gcgctgatcc gcaccccctc gtcgacgacg tcgaggtcga cctcgacggc gtgcaccacg
     4081 acggggtgcg cgagcgggat gaggtcgggg gtgcgcttga cggcctggat gcccgcgacc
     4141 cgggccacgc cgagcgcgtc ccccttgggg accgcgccac cgcgcagcgc cgcgacggcc
     4201 gctgccgaca gcaggaccat gccggatgcc gcggcgcgcc gggcggtgac ctccttggcg
     4261 gagatgtcga ccatgtgggc cgtgccgtcg gcccggacgt gggtgagccc cgacgtggtg
     4321 cgggccttgc tggtggagtc agtcacgcgg gcgatcctgc cccagcagcg gcaggcaccg
     4381 cacgacactc cccgcagcca cgtgctcgac gtcggccggc accacggcga gcgccgtcgc
     4441 cgcggcgagg gcaccgagca cgtgcgaccc ctgcccgccc gacggcgaga tccgcccggc
     4501 ccgctccgtc acgcgcgtga gctcgacctt gccggcgacc gtcggccacc cgacggaggc
     4561 ctcggcctcg aaggagccgt cgtcgtcggg gtcgagcccg gcgaggcggc gcagtaccgg
     4621 caggacgaac acgtggaagg aggcgaagga gctgaccggg ttgccgggca gggtgatgac
     4681 cggggtgcgg tgcgcaccga gcagcccgct gccctgcggc atccccgggc gcatcgcgac
     4741 cttgacgaac tcgacgccac cgtcggcgga cagcacctcc ttgaccgtgt cgtacacgcc
     4801 catcgacacc ccgccgctcg tgatgacgag gtcgggcacg ccgggagggg cgtcgacgag
     4861 ggcgcgcacg gcctccacct cgtcgcgcag gtggaccccg gccgcgacct cggccccggc
     4921 cgcctcgacc agggcccgga gcatgacgtg gttggagtcg acgatctgcc cgtgcgcgag
     4981 ggtggatccg ggggcgacga gctcgtcgcc ggtggacacg acggtgaccc gcggcctcgg
     5041 gtggacgggc agttccgcga cgttggccgc cgcggcgatc gcgaggtgcc ccggggtgag
     5101 ccgggtgccg gcgcggagca ccaccgagcc ggcggtgatg tcctcaccgc ggcggcggac
     5161 gtggcgcccg gcatccggct cgaggaacag cgcgacctgc cccgacccac cgtcggtgag
     5221 ctcgaccggc accacggcgt cacagccctc gggcagcggc gcaccggtca tgatccgcat
     5281 cgtgtggccg gccgcgagga cgtggcgggt cgtgtcgccg gcggcgatgt ctccgctgac
     5341 gggcaggacg accgggttct cgctgctcgc accggcgagc tcactggccc gcacggcgta
     5401 gccgtccatc gccgagttgt cgaacccggg caggtcgacc agggccgtga cgtcctgggc
     5461 cagcaccaac cccagcgcag ccgccacggg cacccgggtg gcgggtagcg gcgagaccag
     5521 ggcgagcacg gcggcccggt gctcctcgag cgtgcgcatc acacgtcccc ctcgaggtgc
     5581 gccccgtcgg ggatcgctcg cacgcccccg gtcacccgca cgtcaccggt gcacacgacg
     5641 tcggcgccga acgtcgcgtc ggcctccacg cgcaggctcg tgcagtcacg gatcgacggg
     5701 acgccgcgcg ggaaccgctg ctcgaaaccc tcgacgtgcc ggtaggccgg cccgaggtcg
     5761 atgaacggct ccgggtgggt gatcgtcgac tcgacctcgc actcgggggt gagccggtac
     5821 aggtccgagc ggatgagcag cagctcgttc gtcgtcttga ccgggcggaa ccggctgcgg
     5881 tcgacggcca gcgcccgcga cccctcgaag agctcgaccg ccgcccccat cgacgactcg
     5941 atctggatga ccggggtgct ctgcggccgg gtcgggtcga cggtcttgcg gttgacgatg
     6001 atcggcagac cgaggatgcc gccgcgctca ccgaggtgcc gcgcgaggac gtcgaggtcg
     6061 atccagaggt tgttcgcgtg gaaccagtgg tgacgctcgg tgtcctggaa gtagtggtcc
     6121 tcgccgggcg cgaccgcggc gctgtcgcgc agcacgagtc gtccgtcgct gcgcctgacg
     6181 gcgaggtgcc cacccttgcg atcgttgtgc gttcgcgggc agacctcggt gacgtagggg
     6241 atgccgttcg ccgccatcca cgcgggaacc gcaccctcgc acgtggcgcc gaggttgtcg
     6301 gcgttggaca ggaaggcgta gcggtagccg cgctggcgca ggctgtcgag caggcccgac
     6361 gtgtagagcg ccacgtagag gtcgccgtga ccgggagggc accactcgag ctcgggatcg
     6421 tccggccact ccaccgggga gaggtcgtcg gcgcgcagct tgggctccat gctctggagg
     6481 aagtccagcg ggagctcgtc gacgggcagg tcgtcgtagc gctcgaggat ctccagggac
     6541 tgctcgcgcg tgcggaagga gttcatcagc agcagcgggg tgtcgacgcc gtggcgtcga
     6601 cgcagcgcca ggacctggcg ggcgatgatg tcgaggaagg tcaggccatc acgcaccggc
     6661 agggccgact tgggcccacg gatccccatg cccgtgccga ggccaccgtt gagcttgatg
     6721 atcaccgtgc gcgccagccc ctcccggatc gcaccggcag atcccgcgac gacgtcgtcg
     6781 agacgggtga cctgctccag cgggctgatg gtgtcctcgg ggatcagtcc ggtcacctgc
     6841 gagcgcaaca gctcgtggaa ccggctgaag acgtcgatcg ccaccccggg aaccccggcc
     6901 gaacgcatct tctccgtggc ggcctccaac ccctgcgtcg tcatgccctt cagcctacga
     6961 tgcctgccat ggcacgtgat ccggtcaaga ccgcgcggcg ggcgagcctg cgtgcgcgcc
     7021 gtcgcgacct cgcggccgcc cgcgacaccg cccgcgacga ccacgacctc gccgggcacg
     7081 tgggcgctct cgtggacacc ctggggttgg gcccgggctc ggtcgtcacg tcgtacgcgt
     7141 cgatccccgg cgagcccccc acggcgaggc tcaacgcgat gctggcggag cgcggcatcc
     7201 gggtcctgct gcccatcacc ctgcccgacc tcgacctcga ctggcacgac gctgccgacc
     7261 ccggcgagca gccgctgggg cgcgagtcgg tcgccgccgt cgacctgatc ctcgcgcccg
     7321 gcctgtccgt cgaccactcg ggcacgcgca tgggccaggg cggcgggtgc tacgaccggg
     7381 ccctcacccg gtgcgacccg cgcccaccga ccgtcgtgct gctccacccg ggcgaactcg
     7441 tcgccgtcga cgacgcacca ctaccgcgcg agccccacga ccgcggcgtc gacgccgtcc
     7501 tgaccgccga gggcctggtc gacctcgggc tcagcccgtg gtcgagccct cggggcgcag
     7561 ggtgagcacg tccgggtcct gggcgcgcac ggcatcctcg gagaacggcc acggccgctg
     7621 cttgcccgag acccagtcct ccacctgatc ggagtatcgg gcgtcagccg ggtggccgct
     7681 caccccggtg gagttgatcc acgtcgaccc gtcgaggttg ccgaggtcga cgaccatccg
     7741 catggacggg ccggccgtca cctcgtaccc gaggctcgcg ttccacccgt tcgcgttgac
     7801 gatggccgac ccgccgggca cgtcgaacgg cccctcgttg accagccagc gcacggcccc
     7861 cggcatcgac tcgccgccga gcaccgggtg ctccagggtc agacggtgca gcttgcccca
     7921 ctgccactgc cccgggtcct tgccgagctc acgggtcagc tcgaggcggg cctggacgag
     7981 ggactgacgc aggatctcgt cgcggctctc ggtgatgttc ggagtgagct tgttgtccca
     8041 ccaggcactg tcggggtcat cgagcaggtc ggtgaccgct gctcgccagc gtgcgccgcc
     8101 gtcggacttc aggcccaccg gaagctcgtc gtcgaagagc agcttcacga ggttgcgcca
     8161 caccgcgttg tagtaggcgg ccgaggcggc ggagtccgag tcccccgcgg gcgtcgtgta
     8221 gtcccacccg cgcagcagct cgcgcgcttc gcgggtgaag tcgagcagct cctggctgat
     8281 ctggtcgccc tcggacgcct cgagggtgac ccccagcagc gccgggacga gcaccttcgc
     8341 gaacgggtcc tggtcgtcga gctggaccgc cgccatctcc accggggaga tcgagttcag
     8401 ccccgagagc cgctcggcga tccgcgtgga gcgccagccc cggtcccagt cggtcgtgag
     8461 gaacggggtc tcccccgcga cgacggcctg gttggcggcg acgatcaccc cgtcagcggg
     8521 gttcagcgcc cacggcatgt cctcgaacgc cacgtacccc gtccagtcgt actcgggatc
     8581 ccaaccgggg gcgggccaga aacccggcgc cgcacgaggg atggcggagc gccgggtcgg
     8641 cacctgcccg ggcgcctggt agccgatgtt gccctcggtg tcggcgtaga cgaggttctg
     8701 cgagggcacc gcgaatgagc gagccgcctc gcggaactcc tggaagttcg tcgccaggcc
     8761 gaggtcgagg atcgcgtcag cggtgcggct cgggatcagc cccgtccagg cgagcgacac
     8821 ggcatacgtc tcgttctcat caccctcctg ctgggtcggg gcccgcccgc cggcgtcgtt
     8881 gaccccctcg acgacgtcgg acatgaccgg tccgtgcacg gtgctgcgca cggtgatccg
     8941 ctggtcggca ccacccctga tcttgatgac ctcctcgcga taggtgatgg gcgcccagtc
     9001 cccgtcgcgc aggtaggtgt cgccgacgac ccgctcgagg tagaagtcgg tgacgtccgg
     9061 cttgaggttg gtcatccccc aggcgatgtc ggcgttgtgg ccgatgatga cgccgggcac
     9121 accggcgaac gagaagccgc tgacgtccag cgggcaggtg ctcgacaccg tgcggcagcg
     9181 caggctgttc tggatccaga cgccgggctg gcccagtgcc aggtgcgggt cgttggcgag
     9241 cagcggcttg ccggtgctcg tgcgggaacc ggccaccacc cacgagttcg acccgatgcc
     9301 ctcgccgcgg ccgagcagct gcggaaccgc gtccagcgca tcctggacga cggcgtaggc
     9361 ctgctgggcg gaagcggaca cgaccgggtc gtccgccgac gccgccgcag cggcaggcgc
     9421 cgcggccgcg cggggcgacg ccacggcatc cgaggtcgtt ggtgcggatg ccgtggcctc
     9481 ggtgagtgcg gacggcaccg acgagctcac cgggcgtccc gcgtcggtcg gggaccactc
     9541 ctggtccgac aggatcgggg ggcgggtcgc ggagtcgtag ggcgggtaga tctgggcgat
     9601 ctgcgccggg gacaggcgcc cgctgagacg cgcccgcgcc agttcgtcct cgtagttgcc
     9661 gcgcaggtcc caggccatcg ccttcagcca cgagatgctg tcgacgggcg tccactcctc
     9721 gatggtcacg tcgggcaggc gcaggccgag cagggtgtac tcgaccgcga cgtcgccggg
     9781 gctgcggccg cgcaggtagg tgttgacccc ctcggcgtag gcctgcagca cctgacgggt
     9841 ctggggcttg agggtgggca gttcctcctc ggcgatccgg cgccatccca tcgtgcgaat
     9901 gaccttgtcg gactcgaggc cgtcggcgcc gacgagctcg gccaggcgcc cggcggtgac
     9961 gtggcgacgc aggtccatct cgaagaagcg ctcctgcgcg tggacgtacc cttgggcacg
    10021 cgcgaggtcg gtgatgctgt cgccgtagag gtgcgggatg ccgctgtcgt cgcgcagcac
    10081 ggagacgtcg gctcccagcc cgggcaggtc ggcggttccg gtggtcttcg gcagcgacgc
    10141 acgcaccgtg gtggtgacga cgaccccggc gacgagggcc gcgacgacga gcacggcgat
    10201 gagtcccaaa aggacacgac ggacgacgac gactcgactc accctgcgag actaggacac
    10261 cgcgacacga cgacacgagg acgggccgtg cgacagacct ggacgacgcc gtttgcggac
    10321 gggggtgcca ccaccgggtt cgacctgcac gacctcaccc tggtgctgct cctcggcagc
    10381 atcgtgctcg tcgtctcggt cgcggccgtg cgcttgtcgg tgcgctcggg gctgccgtcg
    10441 ctgctcatct acctcggtat cggggtgctg ctgggcgagg gcggcttcgg catcaactac
    10501 gactcgcaac agctgaccca ggtgctcggc tacgcggcgc tcgtcctcat cctcgtcgag
    10561 ggtgggctga cgacccggtg gtccggcatc cgccgctccg tggcgccggc ggcggtgctc
    10621 gcgacggtcg gggtcggggt ctccatcgtc gtcgtcgggg tcgcggccaa gctcctgctc
    10681 gggtggtcct gggagatcgc gttcctcgtg gccgccgtcc tggcgtcgac ggatgccgcg
    10741 gcggtgttct cggtgctgcg ccgggtaccg ctcccccgcc agatctcggg gatgctcgag
    10801 gcggagtcgg ggttcaacga cgcgccggtg gtgctgatcg tcaccgcgct cgcggcgtcg
    10861 ctggcccctg gcgcgacacc cgaaccgtgg tggcaggtcg gggtgatcgc cgtgctcgag
    10921 ctcgccgggg ggagcctgat cgggctcgcg atcggttacg gcggcgcgcg cctgttgcgg
    10981 gtggtcgcga cgacgtcgtc ggcgctgttc gccatcgggg tcatcgcggt ggcggtgctg
    11041 gcctacgccg cggccgacgt ggtgcacgcc tccgggttca tcgcgtgcta cctcgccgcg
    11101 ctcgtgctcg gcaacctgcg cctgccccac cgtccggcgg tgacggggtt cgcgacggcg
    11161 atcgggtggt tggcgcagat cgggctcttc gtgctgctcg ggctgctcgc ctcgccgagc
    11221 gggttcgacg acgtgctcgt gccggcgttg gtcgtgggca ccgcgctgct cctgctcggg
    11281 cgcccgttgt cggtcatcgc gtcgtgcgcc ccgttcggca tgtcgttgcg aacccaggcc
    11341 ttcttgtcgt gggccgggct ccgtggggcg gtgccggtcg tgctggccac ggttcccgtc
    11401 acggtggggg ccgttggtgt cgagtggatc ttcgacctcg tcttcgtgct cgtcgtcgtc
    11461 ttcacgatca tccaggcgcc gacgctgccg tgggtggcca ggcaactggg gctgggtgag
    11521 ggccaccacc aggtcgacct ggccgtcgac gccaccccgc tggaggagct cggcgccgac
    11581 ctgctcgaga tcgacgtcgg tcccgggtcg aagatcgcgg gggtgcgggt gttcgagctg
    11641 cgcctgccgg tcggggcgaa cgtgtcgctg gtggtccgtg ggggcacgtc gttcgtgccc
    11701 cgtgagagcg acgtgctgcg ccacggcgac caggtgctcg tcgtcacccc gcaggcggtg
    11761 cgggacgaca ccgagaagcg ccttcaggca gtgagccgtg gcgggcgcct ggccggctgg
    11821 tagc
//
LOCUS       DAOSOC010000068        11747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5426_length_11747_cov_8.010777, whole genome shotgun sequence.
ACCESSION   DAOSOC010000068 DAOSOC010000000
VERSION     DAOSOC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5426_length_11747_cov_8.010777"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..4367)
                     /locus_tag="PL014_06815"
     CDS             complement(105..4367)
                     /locus_tag="PL014_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HOT56836.1"
                     /translation="MVVQPGPHGLGEHDQSGVPPEEIRFGVVMNGGVSLAVWMGGVAL
                     ELDRLVKAGGGSGPYADLLRLTGCTARVDVVSGTSAGGVNGAALALAQVNRFADLASL
                     RDVWVEQGRIEALLRQPFRGAPSSLLKGDDHFLPQLNAAMSRLAEPLDPVPASVAPMD
                     LTMATTVLGGNQEVTVDALGQRLPQTVHAARFRWKRPADATEDTDPFGTRNIGGTAAQ
                     LALAARASSSFPVAFEPTFVPVPAPDEPVADTTGLRPSMAGVVEEWGDTLPPRDRSRF
                     AVDGGVLANTPTRAAVEAIERMPADGPVRRVMLLVYPHAPAPGPDPADVMGEPPTLAG
                     TMTGVLGALSAQGSRTFVDELEDHNVRAASRRGTRADILGGFGEPLPDGSTQTLEGLG
                     AQLFPHYRRLRIWHAARDLARRQVGRRPPASWPEGDDVWDYERIRRAAERAQQEVEDA
                     RRTNGASGAVPVTGIPYLPDHYPTAARPDAGPSWRWGVSGASSIAESVNELLRELVWM
                     LPAGEDWAIARAARQRVTERIRDIQACRHHTDDPWELDEVLAALRPDHHYWMLRLASY
                     ARLMGDGGDAPVREAIARVAHSENQRRFLVSGDKAAADEWQASVAQHLEEVLLRDPKS
                     PAGGAGREVRRLVEEVVEELVSVVPVLRKAAAPRMRTSAHRAGEVLGDPESLQRWHAA
                     LAPGTAAVTVPQLLTTLQQLEVASTTIGDETMTGSTIPIELVQLSAQTRNAFARYSLT
                     GDDKLGGWSVKRFGGFLKRSWRVNDWTWGRLDAATVLCRAVLDPARVRRAAYLSGYLA
                     TDSDPRRLARATVDGVVNSVFAGSGLTSDPRVVALREAAVAEVLGAFLIEDTPTSQLP
                     PSMPALADLFAWGVQLDVVPDEVVALAAAVREDRADGANPRSRGELFLAAEEPLLNRL
                     EKRRREGLSVSGKDRAALLSAFDRAGVGREPLSEETSSDLMIRSASTAGAVSATVLDS
                     PTSGLGALRPVTRLVRGAMLVLHWMIVALTSRGVVPRMLALLGLSVGSILLTASLLGA
                     LPGAWSGVATLFGASCVLAAFSFGALRTGTLLHGLVLLSPLVPLLTFALSQARQAGAA
                     GAVEGAALGGVTLAAMILVAIGLLVLGSLPASYGSVWLALGSLADRRGVPHENTAGRG
                     LVARFFVGAVRRARGLGRSLPGLIGPAVVIALPVALAWWLVDTGAAAIADALLDRRWW
                     LVGLAVVCVVAGGIAAFVLGDLLRPATRVRHPGAQSLTWEYGPLTNPAGINASWAVLY
                     GAGYFTIAIVVLSTAVERGDTREWLVALVVTAIVLGVGLGLLYPVLAPLAALRSMERR
                     EVVRDGQRGGTRFVVADPVGSDVDTARRSYAVDLAERGVAYRWLVSSKPGADGRAPSL
                     APRGVEMLRRLDEARAVPPPGGVRWRLERRFWRVSRRGRGGS"
     gene            complement(4575..5513)
                     /gene="era"
                     /locus_tag="PL014_06820"
     CDS             complement(4575..5513)
                     /gene="era"
                     /locus_tag="PL014_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT56837.1"
                     /translation="MNDDTTEGEQAYVAGFACLVGRPNAGKSTLTNALVGTKVAITSS
                     KPQTTRHTIRGIVSGEHSQLILVDTPGLHKPRTLLGQRLNDLVRETLLEVDVIGFCLP
                     ANQRIGPGDQFIARELAELRRGKRTPVVALATKSDTVDKKRLAEHLIAIDQLGDWSDI
                     VPCSATRGDQVSVVGEVLASHLPPSPGPLYPDDQLTDEPDIVMIAELVREAALEGVRD
                     ELPHSLAVVVEEIVPREGRPDDKPLLDVRVNVFVERDSQKAIVIGRGGSRLREVGTNA
                     RTGIEALLGERVHLDLHVKVAKDWQRDPKQLGRLGF"
     gene            complement(5510..6799)
                     /locus_tag="PL014_06825"
     CDS             complement(5510..6799)
                     /locus_tag="PL014_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT56838.1"
                     /translation="MTRLVVLASVATLLAFLISTAEAAFSRMSRTRAQELLDENRPGA
                     KALVKVVGDTAAYLSVLSFLRVIAESTVAVMVTLAVVERVEGTVEPFLIAIAIMALIS
                     FAVVGVSPGTLGAQHSTKVALAAAPTTVWLRRVLGPFSRLLIALTNAVTPGKGFRDGP
                     FESESELRDLVDMARDHALIEAEEREMLHSVFELGDTLAREVMVPRTDMVSIDDDRTA
                     RAAMSLFLRSGFSRIPVLGKDVDDVVGLLYLKDVMRRLNADPDAGTLPVTELMRPMVF
                     VPESKPVDDLLREMQLDHAHFAVVVDEYGGTAGLVTIEDILEEIVGEITDEYDREAPE
                     VENLDDGRVRVAATMHVDDLAELFEVALEEEEVDTVGGLIAKELGRVPILGSTCEVAG
                     LRLTAERMSGRRHRIATVIVERVEPAADAPEMEERAG"
     gene            complement(6816..7283)
                     /gene="ybeY"
                     /locus_tag="PL014_06830"
     CDS             complement(6816..7283)
                     /gene="ybeY"
                     /locus_tag="PL014_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT56839.1"
                     /translation="MSIDVLNETEFSIDELELVACARYVMEQMRVHPKADLCVRLVDE
                     PAMETLHVQWMDLPGPTDVMSFPMDELRPGREGVEPQEGMLGDIVLCPSVAARQAREA
                     GHATEEELLLLTTHGILHLLGFDHAEPDEEREMFELQRQLLLTFLATRGRPTA"
     gene            complement(7280..8281)
                     /locus_tag="PL014_06835"
     CDS             complement(7280..8281)
                     /locus_tag="PL014_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT56840.1"
                     /translation="MTEPQTHPHVREHTVVVPADVPMVALLGPRDELLRTMERQFPRV
                     DLHVRGNEFHVSGPSAEVALVERLIDELLDVLEGGQALSGDAVERSIAMLRAQTVERP
                     ADVLTMNIVSSRGRTIRPKTLGQKRYVDAIDSNTVVFGIGPAGTGKTYLAMAKAVAAL
                     QAKQVNRIILTRPAVEAGERLGFLPGTLNDKIDPYLRPLYDALHDMVDPESIPRLMAS
                     GTIEVAALGYLRGRTLNDAFIILDEAQNTTAEQMKMFLTRLGFGSQMVVTGDVTQVDL
                     PSSATSGLRVVRDILTDIDDVAFCELTASDVVRHRLVAAIVDAYGRYDERQAPAVRR"
     gene            complement(8334..8681)
                     /locus_tag="PL014_06840"
     CDS             complement(8334..8681)
                     /locus_tag="PL014_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOT56841.1"
                     /translation="MSETESCLFCRIVAGQIPATVVAESAHSLAFRDIEPGAPTHVLV
                     VPRRHVPDLAGLAAASAAELADAVALAAQVADDEGLPGYRVVANTGEAAGQSVFHAHL
                     HVLGGRSLTWPPG"
     gene            complement(8692..9435)
                     /locus_tag="PL014_06845"
     CDS             complement(8692..9435)
                     /locus_tag="PL014_06845"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOT56842.1"
                     /translation="MTLPLHLLPTDALAGRGVGDVVVLDGPEGRHAATVRRTRVGEHL
                     LLTDGAGLRVEAEVIAVGSGSLDLRVVSVTSDPEPSPRLVLVQALAKNDRDDQAIEAA
                     TECGVDEVVPWQASRSVVQWRGERGEKAWRKWDAVLVAATKQSRRTRRPVLAPTATSA
                     QLSARIGAAAAAFVLHEDARMPLAGVTLPASGDVVVVVGPEGGISPEELAAFEAAGGV
                     PVRLGDSVLRSSSAGPAALAVLSAMSRWR"
     gene            complement(9452..10579)
                     /gene="dnaJ"
                     /locus_tag="PL014_06850"
     CDS             complement(9452..10579)
                     /gene="dnaJ"
                     /locus_tag="PL014_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT56843.1"
                     /translation="MNDYYADLGVARDASPEDIKRAYRKAARRLHPDVNPGPEAEEQF
                     KKVSQAYDVLSDADKKRSYDAGADPYGAGAAGFGQGFSFSDIMDAFFGGQAGTPGRGP
                     RSRTTRGQDALVRLDIDLGDAVFGAQKDLTIDTAVGCGTCHGAGMQPGTSTRTCDVCG
                     GRGEVQQVQRSFLGQVMTTRPCMTCQGFGQIITNPCYECSGDGRVRTRRTLTLKVPAG
                     VDTGTRIQLAGEGEVGHGAGPAGDLYVEVAVNRHPVFQRRGNDLHATIEVPMTAAALG
                     ASLMMETFDGPQTLEVRRGTQSGDTITLRGLGVTALRGTGRGDIIVHAMVQTPTRIDA
                     EQEALLRQLAQLRGEERPEGKVANPGDGSIFGKLRDAFKAR"
     gene            complement(10668..11699)
                     /gene="hrcA"
                     /locus_tag="PL014_06855"
     CDS             complement(10668..11699)
                     /gene="hrcA"
                     /locus_tag="PL014_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOT56844.1"
                     /translation="MNEDRRLRVLRAIVQDYVQTSEPVGSKALLERHQLGVSAATVRN
                     DMAALEDEGLIAAPHTSAGRVPTDAGYRLFVDRLSAVKPMSSAEQRAIAHFLEGALDL
                     DDVVDRTVRLLSSLTHQVAVVQYPSLARSCVRHIELVPLGGDRVLVVLITTTGRVEQR
                     VVDVGRDLSSTVGEDTLATLRTELNRATAGRTLAEASAELAAAPEQLEPGLRDLVRAV
                     GTALQDSFVEEREERVVLAGTANLARVGPDFTTSLGPVLEALEEHVVLLKLLGTAAEQ
                     GEQVAVRIGHENPYAGLQTTSMVATGYGAGSDLVAGLGVLGPTRMDYPTTMAAVRTVA
                     TYVSRILAQ"
BASE COUNT         1683 a         4209 c         4258 g         1597 t
ORIGIN      
        1 cgatcaacaa gcccagtgag ccacgaaagc agctgtggca caacgacttt caaactattt
       61 tcttaccgtt ttcggggccc cttccagact ccttccgggc cccctcacga gcccccgcgg
      121 ccgcgtcgag atacccgcca gaaccgacgt tcaaggcgcc accgcacccc gccgggaggc
      181 ggcaccgcgc gcgcctcatc gagccgccgc agcatctcca ccccacgcgg agcgagcgac
      241 ggggcccgcc cgtcagcacc gggcttcgac gacaccagcc agcgataggc gaccccacgt
      301 tcggcgaggt cgacggcata cgaccgccga gccgtgtcga cgtctgagcc gaccgggtcg
      361 gcgacgacga accgcgtgcc accccgttgc ccgtcacgca ccacctcgcg gcgctccatc
      421 gaccgcaacg ccgccagcgg ggcgagcacc gggtagagca gccccagccc cacgcctagc
      481 acgatcgccg tgacgaccag cgccaccagc cactcgcgcg tgtcgccgcg ctccaccgcc
      541 gtcgacagca ccacgatcgc gatcgtgaag taccccgccc cgtagagcac cgcccacgac
      601 gcgttgatgc ctgccgggtt ggtgagcggc ccgtactccc aggtgagcga ctgcgccccg
      661 gggtgccgca cccgagtggc cggccgcagg aggtcaccga ggacgaacgc cgcgatcccc
      721 ccggcgacca cacacaccac cgcgagcccc accagccacc accgcctgtc cagcaacgcg
      781 tcggcgatgg cggcggcccc ggtgtcgacg agccaccacg cgagcgccac gggcagggcg
      841 atgaccaccg ccggcccgat cagccccggc agcgagcgac ccagaccccg cgcgcgtcgc
      901 acagccccga cgaagaagcg ggcgaccagc ccacgcccgg ccgtgttctc gtgcgggacc
      961 ccacgccgat ccgccagcga gcccagggcc agccagacgc tgccgtagga cgccggcagg
     1021 ctcccgagca cgagcagccc gatcgcgacg aggatcatcg cggccaacgt gacaccaccc
     1081 agcgccgcgc cctcgaccgc cccagcggcc ccggcctgcc gtgcctgcga cagggcaaag
     1141 gtcaacaggg gcacgagcgg gctcagcagc accagcccgt gcagcaacgt ccccgtgcgc
     1201 aaggcaccga acgagaaggc cgcgagcacg cacgacgcac cgaacagggt cgcgacgccc
     1261 gaccaggccc ccggcagggc cccgagcaac gaggcagtca gcaggatgct gcccactgac
     1321 aacccgagca gcgccagcat ccgcggcacc accccgcgcg aggtgagcgc aacgatcatc
     1381 cagtgcagga cgagcatcgc gccccgcacg aggcgggtca ccgggcgcag cgccccgagc
     1441 cccgacgtgg gcgagtccag caccgtcgcc gacaccgccc cggccgtcga ggcactgcgg
     1501 atcatcaggt cgctcgacgt ctcctcggag agcggttcac gccccacccc ggcgcggtcg
     1561 aaggcgctga gcagcgccgc ccggtccttg cccgacaccg acagtccctc acgacggcgc
     1621 ttctccaacc ggttgagcaa cggctcctcg gcggcgagga agagctcacc gcgcgatcgc
     1681 gggttggccc cgtcggcgcg gtcctcgcgc accgccgcgg cgagcgccac cacctcgtcg
     1741 ggcacgacgt cgagctgcac cccccacgcg aagaggtcgg ccagggccgg catcgacggc
     1801 ggaagctggc tggtgggggt gtcctcgatg aggaaggctc cgagcacctc ggcgacggcc
     1861 gcctcccgca gggcgacgac ccggggatcg gacgtcaggc ccgagccggc gaacaccgag
     1921 ttgacgacgc cgtcgaccgt ggcccgcgcc aggcgccgcg ggtcggagtc ggtcgcgagg
     1981 tatcccgaca ggtacgcggc gcgccgaacc cgggccgggt cgagcaccgc ccggcacagc
     2041 acggtcgcgg catccaggcg cccccacgtc cagtcgttga cccgccacga gcgcttgagg
     2101 aacccgccga agcgcttcac cgaccagcca ccgagcttgt cgtccccggt cagggagtac
     2161 cgcgcgaacg cgttgcgggt ctgcgccgac agctgcacga gctcgatcgg gatcgtcgag
     2221 ccggtcatcg tctcgtcgcc gatcgtcgtg ctggccacct cgagctgctg gagggtcgtc
     2281 agcagctggg gcaccgtcac ggcggccgtc ccgggcgcga gcgcggcgtg ccaccgctga
     2341 agggactccg ggtcgccgag cacctcaccc gcgcggtggg ccgacgtcct catccgcggc
     2401 gcggctgcct tgcgcagcac cggcacgacg gacaccagct cctcgacgac ctcctcgacg
     2461 agccgtcgga cctcgcgacc tgcgcctccg gcggggctct tcgggtcccg cagcagcacc
     2521 tcctcgaggt gctgggcgac gctggcctgc cactcgtcgg ccgcggcctt gtcgccgctg
     2581 acgaggaagc ggcgctggtt ctccgagtgc gccacccggg cgatcgcctc gcgcaccggg
     2641 gcgtctcccc catcccccat gagccgcgcg tacgaggcga gtcgcagcat ccagtagtgg
     2701 tggtccgggc gcagcgcggc cagcacctcg tcgagttccc aggggtcatc ggtgtggtgg
     2761 cggcaggcct ggatgtcacg gatgcgttcg gtcacccgct gccgggcggc tcgggcgatc
     2821 gcccagtcct cccccgccgg cagcatccac acgagctcgc gcagcagctc gttgaccgat
     2881 tccgcgatcg acgacgcgcc gctcaccccc cagcgccagg agggaccggc atccggcctc
     2941 gcagcggtcg ggtagtgatc ggggaggtag gggatgccgg tcacgggcac cgcgcccgac
     3001 gcaccgttcg tgcgacgcgc gtcctcgacc tcctgctgcg cccgctcggc agcacggcga
     3061 atgcgttcgt agtcccagac gtcgtcaccc tccggccagg atgcgggggg tcggcggccg
     3121 acctgccggc gggcgaggtc tctcgcggcg tgccagatgc gcaggcggcg gtagtggggg
     3181 aagagctgcg cgccgagccc ttcgagggtc tgtgtgctgc cgtcgggcag cggctcgccg
     3241 aacccaccga ggatgtccgc gcgcgtaccc cggcggctcg cggcgcgcac gttgtggtcc
     3301 tcgagctcgt cgacgaaggt gcggctgccc tgcgcggaga gcgcgccgag gacccctgtc
     3361 atcgtgcccg cgagggtcgg gggctccccc atgacgtctg ccgggtcagg gccgggggcg
     3421 ggtgcgtgcg ggtagacgag cagcatgacc cggcgaacgg ggccgtctgc cggcatccgc
     3481 tcgatggcct cgaccgccgc cctggtcggg gtgttcgcga gcacgccgcc gtcgacggcg
     3541 aaccgtgagc ggtcgcgcgg cgggagggtg tcaccccact cctcgacgac cccagccatc
     3601 gacgggcgca gcccggtcgt gtcggccacg ggctcatcgg gcgcgggcac cggcacgaag
     3661 gtgggctcga acgccaccgg gaagctcgac gacgcccggg ccgcgagcgc cagctgggcc
     3721 gcggtgccgc cgatgttgcg ggtgccgaac gggtcggtgt cctcggtggc gtcggcgggg
     3781 cgcttccacc ggaaccgcgc cgcgtggacc gtctggggca gccgctggcc gagggcgtcg
     3841 accgtgacct cctggttgcc gcccagcacg gtcgtcgcca tggtcaggtc catcggggcg
     3901 acggacgcag gaaccgggtc gagcggttcg gccagtcgcg acatcgccgc gttcagctgc
     3961 gggaggaagt ggtcgtcacc cttgagcagc gagctcgggg ctccgcggaa cggctggcgc
     4021 agcaacgcct cgatgcggcc ctgctccacc cagacgtcgc gcagcgaggc gaggtcggcg
     4081 aagcggttga cctgggccag ggcgagggcg gcgccgttga cacccccggc cgacgtgccg
     4141 gacacgacgt cgacccgggc tgtgcacccg gtgaggcgca gcaggtctgc gtagggcccc
     4201 gatcccccgc cagccttgac gaggcggtcg agctcgaggg cgaccccacc catccagacg
     4261 gcgagggaga ccccgccgtt catgacgacg ccgaagcgga tctcctccgg gggcacgccg
     4321 gactggtcgt gctcaccgag accgtgcgga ccgggctgga cgaccatggc tcactcctgc
     4381 cactggggta ccgaaaccgc tgaggtgctc agacaccagc gtcctcggtc accgtggcac
     4441 cggccatcgt ccgatcatcc acccgtgagg agtggccggg ggccgcggtg ataccacccc
     4501 tcagaagccg aggcggccga gctgcctgcg taccccgagt gacctattgg tcgatctcgg
     4561 gacggtgcga gccgtcagaa gccgaggcgg ccgagctgct tggggtcgcg ctgccagtcc
     4621 ttggcgacct tgacgtgcag gtcgaggtgc acgcgctccc cgaggagtgc ctcgatgccg
     4681 gtgcgcgcgt tcgtgccgac ctcgcgcagc cgtgacccac cccgaccgat gacgatggcc
     4741 ttctgcgagt cgcgctcgac gaagacgttg acccgcacgt cgagcagcgg cttgtcgtcg
     4801 gggcggccct cgcgcggcac gatctcctcg acgacgacgg ccaggctgtg cggcagctcg
     4861 tcgcgcactc cctcgagcgc ggcctcgcgc acgagttcgg cgatcatcac gatgtcgggc
     4921 tcgtcggtca gctggtcgtc ggggtagagc ggcccgggcg agggcggcag gtggctggcc
     4981 agcacctcac cgacgaccga gacctggtcg ccgcgggtgg ccgagcaggg gacgatgtcc
     5041 gaccagtcgc cgagctggtc gatggcgatg aggtgctcgg cgagccgctt cttgtcgacc
     5101 gtgtccgact tcgtcgccag cgccacgacc ggtgtgcgct tgccccgccg cagctcggcg
     5161 agctcgcggg cgatgaactg gtcgcccggg ccgatccgct ggttcgcggg caggcagaac
     5221 ccgatgacgt cgacctcgag cagcgtctcg cgcacgaggt cgttgaggcg ctgccccagc
     5281 agggtgcgcg gtttgtgcag cccgggggtg tccacgagga tcagctggct gtgctccccc
     5341 gacacgatgc cgcggatcgt gtgccgggtg gtctgcggct tgctcgacgt gatggcgacc
     5401 ttggtgccga cgagcgcgtt ggtcagggtc gacttgccgg cgttgggccg cccgaccagg
     5461 cacgcgaacc cggcgacgta cgcctgctcg ccctcggtgg tgtcgtcgtt catccggccc
     5521 gctcctccat ctccggggcg tctgccgccg gctcgacccg ctcgacgatc acggtggcga
     5581 tgcggtgacg tcgccccgac atgcgttccg cggtgagacg caaccctgcc acctcacacg
     5641 tcgacccgag gatcgggacc cgcccgagct ccttggcgat gaggccaccg acggtgtcga
     5701 cctcctcctc ctcgagggcc acctcgaaca gctcggcgag gtcgtcgacg tgcatcgtcg
     5761 cggcgacccg gacccggccg tcatcgaggt tctcgacctc gggtgcctcg cggtcgtact
     5821 cgtcggtgat ctcgccgacg atctcctcga ggatgtcctc gatggtcacc agcccggccg
     5881 tgccgccgta ctcgtccacg acgacggcga agtgcgcgtg gtcgagctgc atctcacgca
     5941 gcaggtcatc gaccggcttg ctctcgggca cgaacaccat ggggcgcatg agctcggtga
     6001 cggggagcgt cccggcatcc gggtcggcgt tcagccgccg catgacgtcc ttgaggtaga
     6061 gcaaccccac cacgtcgtcg acgtccttgc cgagcacggg gatgcgcgag aagccggagc
     6121 gcaggaacag gctcatcgcc gcgcgagcgg tgcggtcgtc gtcgatggac accatgtcgg
     6181 tgcgcggcac catcacctcg cgggccaggg tgtcgccgag ctcgaagacc gagtgcagca
     6241 tctcgcgctc ctcggcctcg atgagggcgt ggtcgcgggc catgtcgacg aggtcgcgca
     6301 gttcgctctc gctctcgaac gggccgtcgc ggaacccctt gcccggggtg accgcgttgg
     6361 tgagcgcgat gagcagccgc gagaacggcc cgagcacccg gcgcagccac accgtcgtcg
     6421 gtgcggctgc cagggccacc ttggtgctgt gctgcgcacc gagcgttccc ggtgacaccc
     6481 cgacgacggc gaaggagatg agcgccatga tcgcgatggc gatgaggaag ggctcgaccg
     6541 tgccctcgac ccgttcgacc acggccagcg tcaccatcac cgccacggtc gactcggcga
     6601 tgacgcgcag gaacgacagc accgacaggt aggccgccgt gtcgccgacg accttgacca
     6661 gggccttggc gcccgggcgg ttctcgtcga gcagctcctg ggcgcgggta cgcgacatcc
     6721 gcgagaaggc ggcctcggcg gtggagatga ggaaggcgag cagggtcgcg acggacgcga
     6781 ggacgacgag gcgggtcatg gtcttttctg ggtggtcagg cggtgggtcg cccgcgcgtg
     6841 gcgaggaagg tgaggagcag ctggcgctgc agctcgaaca tctcgcgctc ctcgtcgggt
     6901 tcggcgtggt cgaagccgag gaggtgaagg atgccgtggg tcgtcaggag cagcagctcc
     6961 tcctcggtcg cgtggccggc ctctcgggcc tgacgggctg cgacgctcgg gcacaggacg
     7021 atgtcaccga gcatgccctc ctgcggctcg acaccctcgc gcccgggacg aagctcgtcc
     7081 atcgggaagc tcatgacgtc ggtcgggccc ggcaggtcca tccactgcac gtgcagcgtc
     7141 tccatggcgg gttcgtcgac gaggcgcacg cacaggtcgg ccttggggtg gacccgcatc
     7201 tgctccatca cgtaccgggc acaggcgacg agctcgagct cgtcgatcga gaactcggtc
     7261 tcgttgagga cgtcgatgct caccggcgca ccgccggcgc ctggcgttcg tcgtagcggc
     7321 cgtaggcgtc gacgatcgcc gcgacgagcc ggtgacgaac gacgtcggat gccgtgagct
     7381 cgcagaacgc gacgtcgtcg atgtcggtga ggatgtcgcg cacgacccgc agcccgctcg
     7441 tcgccgagct cggcaggtcg acctgggtga cgtcgccggt gacgaccatc tgcgagccga
     7501 acccgaggcg ggtgaggaac atcttcatct gctcggcggt ggtgttctgc gcctcgtcga
     7561 ggatgatgaa cgcgtcgttg agcgtgcgcc cacgcaggta ccccaaggcc gccacctcga
     7621 tcgtgcccga ggccatcagc cgggggatcg actcggggtc gaccatgtcg tgcagcgcgt
     7681 cgtagagcgg gcgcaggtag gggtcgatct tgtcgttgag ggtgccgggc aggaacccga
     7741 gccgctcccc cgcctcgacg gccgggcggg tgaggatgat gcggttgacc tgcttggcct
     7801 gcagcgctgc cacggccttg gccatcgcga ggtaggtctt gccggtaccg gccgggccga
     7861 tgccgaagac gaccgtgttg gagtcgatgg cgtcgacgta gcgcttctgg cccaacgtct
     7921 tcggccggat cgtgcgcccg cggctggaga cgatgttcat cgtcagcacg tcggcggggc
     7981 gctcgacggt ctgggcgcgc agcatggcga tcgagcgctc gacggcgtca ccgctgagcg
     8041 cctggccgcc ctcgagcacg tcgagcagct cgtcgatgag gcgctcgacg agcgcgacct
     8101 cggcgctcgg gccggagacg tggaactcgt tgccccgcac gtgcaggtcg acccgcggga
     8161 actggcgctc catggtgcgc agcagttcgt cgcgaggacc cagcagggcg accatcggca
     8221 cgtcggcggg gacgacgacg gtgtgttcgc ggacgtgggg gtgggtttgc ggctcagtca
     8281 tcgtgggaca agtgtacgag gccctgcccg ggtgcagcac ctgagattgc cggtcagccc
     8341 ggaggccacg tcagggatcg cccgccgagc acgtgcaggt gggcgtggaa cacgctctgc
     8401 ccggcggcct ctccggtgtt cgcgaccacc cggtagcccg gcaacccctc gtcgtccgcg
     8461 acctgcgcgg cgagtgccac ggcatccgcg agctccgccg cggacgcggc ggccaacccc
     8521 gcgaggtcgg gcacgtgacg gcgcgggacg acgagcacgt gggtcggcgc gcccggctcg
     8581 atgtcgcgaa aggccaggct gtgtgcactc tcggcgacga cggtcgcggg gatctgcccc
     8641 gcgacgatcc ggcagaacag gcagctctcg gtctcgctca tgccgccgaa cctaccgcca
     8701 gcgggacatc gcgctgagga cggccagggc cgccggcccc gcgctcgacg agcgcagcac
     8761 gctgtcgccg agacggaccg ggactccccc ggcggcctcg aaggccgcga gctcctcggg
     8821 cgagatgccg ccttccggtc cgacgacgac cacgacgtca ccggatgccg ggagcgtcac
     8881 ccccgcgagc ggcatccggg cgtcctcgtg gagcacgaag gccgcggcgg cggcgccgat
     8941 ccgggcgctg agctgcgccg acgtggcggt gggcgccaac accggtcggc gggtgcggcg
     9001 ggactgcttg gtggcggcga cgagcacggc atcccacttg cgccaggcct tctcgccgcg
     9061 ctccccgcgc cactgcacga cgctgcgaga ggcctgccac ggcacgacct cgtcgacacc
     9121 gcactcagtc gcggcctcga tggcctggtc gtcacggtcg ttcttggcga gcgcctggac
     9181 gagcaccagg cgagggctcg gctcggggtc gctcgtcacc gagacgacgc gaaggtccag
     9241 gctgcccgac ccgaccgcga tcacctcggc ctcgacgcgc agcccggcgc cgtcggtgag
     9301 caacaggtgc tcgccgacgc gggtgcggcg aacggtggcg gcgtgccgac cctcggggcc
     9361 gtcgagcacc acgacgtcgc cgacgccgcg acccgccaag gcgtcggtcg gcagcaggtg
     9421 cagcggcagc gtcacggggg tggcctgccg gtcagcgggc cttgaacgcg tcgcgcagct
     9481 tgccgaagat cgacccgtca ccggggttgg cgaccttgcc ctcgggccgc tcctcgccgc
     9541 gcagctgggc gagctgacgc agcagcgcct cctgctcggc gtcgatcctg gtcggggtct
     9601 gcaccatggc gtgcacgatg atgtcgccgc gaccggtgcc gcgcagcgcg gtgacaccga
     9661 gcccgcgcag cgtgatggtg tccccggact gggtgccgcg gcgcacctcc agggtctgcg
     9721 gcccgtcgaa ggtctccatc atcagcgacg cgccgagtgc ggccgcggtc atcggcacct
     9781 cgatcgtggc gtgcaggtcg ttgccgcgac gctggaagac ggggtggcgg ttgacggcga
     9841 cctcgacgta caggtcaccg gccgggcccg ctccgtggcc gacctcgccc tcaccggcga
     9901 gctggatgcg ggtgcccgtg tcgaccccgg ccgggacctt gagggtcagg gtgcgacggg
     9961 tgcgcacccg gccgtcgccg gagcactcgt agcaggggtt ggtgatgatc tggccgaagc
    10021 cctggcaggt catgcagggg cgggtcgtca tgacctggcc gaggaagctg cgctgcacct
    10081 gctgcacctc gccgcgaccg ccgcagacgt cacaggtgcg ggtcgaggta cccggctgca
    10141 tcccggcgcc gtggcaggtg ccacacccga cggcggtgtc gatggtgagg tccttctggg
    10201 cgccgaacac ggcatccccg aggtcgatgt cgaggcgcac cagggcgtcc tgaccgcggg
    10261 tggtgcgcga gcgcgggccg cggcccggcg tgccggcctg gccaccgaag aaggcgtcca
    10321 tgatgtcgct gaaggagaag ccctgaccga acccggccgc gcccgccccg taggggtcgg
    10381 cgcccgcgtc gtaggagcgc ttcttgtcgg cgtcggacag cacgtcgtag gcctgggaga
    10441 ccttcttgaa ctgctcctcg gcctcggggc cggggttgac gtccgggtgc agtcgccggg
    10501 cggccttgcg gtaggcgcgc ttgatgtcct cggggctcgc gtcgcgggcg acaccgaggt
    10561 cggcgtagta gtcgttcacg ggtgaagggt ccttcgggac gtggttgagg cggtggatgc
    10621 cgtgggtgca attggtgcgg tgggtgcggt gctggcaggc cggggtgtca ctgcgcgagg
    10681 atccgcgaga cgtaggtggc gacggtgcgc acggcagcca tcgtcgtggg gtagtccatc
    10741 ctcgtcggtc cgaggacccc gaggccggcg acgaggtcgg agccggcgcc gtagccggtc
    10801 gcgaccatcg aggtggtctg caaccccgca tacgggttct cgtggccgat gcggacggcc
    10861 acctgctccc cctgctcggc ggcggtgccg agcagcttga gcagcacgac gtgctcctcg
    10921 agcgcttcga ggacggggcc gaggctggtc gtgaagtccg ggccgacccg ggcgaggttg
    10981 gcggtgccgg ccagcacgac ccgctcctcg cgttcttcca cgaaggagtc ctgcagcgcg
    11041 gtgccgacgg cgcgcacgag gtctcgcagt ccgggctcga gctgctcggg cgcggcggcg
    11101 agctcggccg acgcctcggc cagggtacgc cccgccgtgg cgcggttgag ctcggtgcgc
    11161 agcgtggcga gggtgtcctc gccgacggtg ctgctgaggt cgcggccgac gtcgacgacc
    11221 cgctgctcga cccgccctgt cgtcgtgatg agcacgacga gcacccggtc accgccgagc
    11281 ggcacgagct cgatgtggcg cacgcacgag cgcgcgagag aggggtactg cacgacggcg
    11341 acctggtggg tcagcgacga cagcagtcgc accgtgcggt ccacgacgtc gtcgaggtcg
    11401 agggcaccct cgaggaagtg ggcgatggcg cgctgctcgg cgctgctcat cggcttcacg
    11461 gccgagagac ggtccacgaa gagccggtag ccggcatccg tgggcacccg cccggcactg
    11521 gtgtgcgggg ccgcgatcaa cccctcgtcc tcgagagccg ccatgtcgtt gcgcacggtc
    11581 gcggccgaca ccccgagctg gtgacgctcg agaagggcct tcgaccccac gggttcggac
    11641 gtctgcacgt agtcctgcac gatggcgcgc agcacgcgca accgacggtc ctcgttcatg
    11701 cccacctccg gtccttcgcc tggacagcct ttggcactct cgcgcca
//
LOCUS       DAOSOC010000069        11733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5438_length_11733_cov_8.506251, whole genome shotgun sequence.
ACCESSION   DAOSOC010000069 DAOSOC010000000
VERSION     DAOSOC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5438_length_11733_cov_8.506251"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..689
                     /locus_tag="PL014_06860"
     CDS             111..689
                     /locus_tag="PL014_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOT56845.1"
                     /translation="MSSTTRSISRALTLGAAATLVFTLTACGQDTPTPTGATASSSSS
                     TTAETTQGAGAVVLDDGWVKATDDAMAGMEPMTAAFGTLRNTTDHEVTITGGSSPAAG
                     MVELHETVKTDTGAMQMQPKAGGIVIPAGGTHLLEPGSDHLMLMKLTGPLETGTTTTL
                     TLSTSDGDVTLTVPVRAFTGAQESYAPSPTHS"
     gene            686..1921
                     /locus_tag="PL014_06865"
     CDS             686..1921
                     /locus_tag="PL014_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HOT56846.1"
                     /translation="MSTPDQGRAAQSGRRSVLLSGLAGAGLAATAAIATAASGGPSAS
                     ATSAVPPGTRATGPDAVARPFRGAHQAGILEAPPAYATFVALDLVDGADADVVRRLLT
                     VWTDDIERLMAGRGTLTDQEPELASVTAELTVTVGVGRRVVEVVGAQVPAWLAPLPAF
                     AIDRLQPRWSGGDLLLQICATSPTTVAHAQRRLLAGAAGLASVRWVQRGFREPHEGPG
                     VPMRNLFGQVDGTVQPDVAGLDADLLWSADAGPTWLRGGSSVVVRRIEMDLDGWDRVD
                     RLSRENSIGRRLATGAPVTGGGLDTAPDLEAVDGLGFHVVDDAAHIRRAHASAPHERF
                     LRRPYSYDDAPDAGSTSSSGLVFVAYQADPVRQFVPVQARLAELDLLNLWTTPIGSAV
                     FAVLPGAQEGEVLGQALFT"
     gene            complement(2055..2140)
                     /locus_tag="PL014_06870"
     tRNA            complement(2055..2140)
                     /locus_tag="PL014_06870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2105..2107),aa:Leu,seq:cag)
     gene            complement(2271..3290)
                     /locus_tag="PL014_06875"
     CDS             complement(2271..3290)
                     /locus_tag="PL014_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOT56847.1"
                     /translation="MNDGVQVIVPREVPLGGLRSMTVRRTLPARERSFVGAWCFVDHY
                     GPDDVEVTGGMDVAPHPHCGLATVSWLFTGEVEHRDSLGTVATVRPGEVNLMTAGRGI
                     SHSEVSTAGTAVLHGVQLWVVLPAASAGCEPRFEHHVPAVVSVAPGVAASVFVGSLFG
                     STSPVSVETPLVGAEVALEPGAVVDVPVDAGFELGVLVDSGSVVFAGQRLARGELGVV
                     EAGDFGDGSGRGGGSDAGGAPRHPGSVALQAGSDGARVVVLGGEPFAEEVVMWWNFIG
                     RSHDEVVAAREEWEASGVPGASVGRFGSVQGYVGEVSRIPAPGLPGVRLRSRGNRSAG
                     GRSRG"
     gene            3387..4283
                     /locus_tag="PL014_06880"
     CDS             3387..4283
                     /locus_tag="PL014_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HOT56848.1"
                     /translation="MGLFDRLEAGIERAVQGTFAKHLRSAVHPVEIASTIRRAMDDRA
                     VSSSGRAIVPNVFTVELSPGDYDRLHPDLADVEMDLVAAAEEHCEGQRYQPAGPFDII
                     FEEHDDLETGVFRIRPSKASRPRATGTTGSERAVQRPSTPDAAGPPPAHVAPRAAVPP
                     PSAPPAPAAPVARAPRAPVAREPEPTPAPPVAAAPRRVNPADRPWLDVDGERYPLMGA
                     ITILGRDDVADIILDDPGISRRHSELRVTTDGPRFVTTIRDLGSTNGTYVNGERITSE
                     HLQDGDRITVGRTSITFRGGRR"
     gene            4294..4854
                     /locus_tag="PL014_06885"
     CDS             4294..4854
                     /locus_tag="PL014_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT56849.1"
                     /translation="MSELTITLMRLGLLVLLWFFVFAIVGVLRGDLYGTRVGRRSSGR
                     AKAAKPAKPAPVAVVAGPAAGGAVATAPAQAPARGGRFGRRGGGKGPTRLTVTAGQLA
                     GTTLPLRPAGTLIGRSPECALVLDDDYVSGRHARIFQADDGTWQVEDLRSTNGTFLGA
                     NRLTEPREVAVGSVLRIGQTVVELQR"
     gene            4858..6291
                     /locus_tag="PL014_06890"
     CDS             4858..6291
                     /locus_tag="PL014_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOT56850.1"
                     /translation="MPFAFHYAARSDVGMVRSNNEDSAYAGPHLLALADGMGGHAGGD
                     VASSTVIAGLVSLDDEALSGRDLSEALLARILSANADIGNEVRSEPRLDGMGTTLIAM
                     LRSGDKIVLAHIGDSRAFMVRDGVVTQVTKDHSFVQTLVDEGRITAEEAQTHPQRSLV
                     TRVLTGSVDDEPDLVVRQGRIGDRYLLASDGLTDYVARDTIDEVLTAPGSPADCADRL
                     VALALRAGAPDNVTVIVSDIVDGTTDSAPPTQPQVVGAAAVREKGTRPMPTTPAAKAA
                     ALTQEATRAASGSGTDEPDAGGDGVTLAEEGPRSHGGTVLRLLALIVVSAVVLAGGSY
                     AAYAWSQQQYHLSGNDGVVTVYQGVNQTLGPISLSRPLYTTTIAVDDLPPSYQSSLEQ
                     GIDVDDRAAADARVEDLRLQATACRWARLNGEECRTVPSTWTPPSPTPTLTPTPSPSG
                     TPSGTTPAPTPTTTSRGPSPTGSPVTA"
     gene            6288..7670
                     /locus_tag="PL014_06895"
     CDS             6288..7670
                     /locus_tag="PL014_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOT56851.1"
                     /translation="MSVVSSYTPRRGRNVELVLLIAAVAIVLLAYVNVGVATSESMPP
                     GTMALAGGYLAMAVGFHLVLRWRASYADPLMLPIITLLNGLGLVMIHRIDLANGTSIS
                     DGVALRQLMWTGVAVVIAAAVLVVLRDHRVLRRYTYLAGVVGFVLLLLPLMPVIGTSI
                     YGARIWIRIGSMSFQPGEIAKIALAIFFAGYLVQTRDALSVAGRKVLGLTLPRARDLG
                     PILIAWLASLAVLVFERDLGSSLLFFGLFVAMLYVATERVSWIAIGLTLFGAGAYVAY
                     LMFGHVQTRVLLWLDTFSPEALQASDQLAKGLMGMAAGGMFGTGLGRGRPDLTYFAES
                     DFIIPSFGEEIGLVGLFALLMLYVLLVQRGLRTSLGTRDGFGKLLAAGLSFSLALQCF
                     VVVGGVTRVIPLTGLTMPFLSSGGSSLLANWTLVAILLRISDHARRPMPEINVPHEVG
                     QAHTEVVRMP"
     gene            7667..9118
                     /locus_tag="PL014_06900"
     CDS             7667..9118
                     /locus_tag="PL014_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT56852.1"
                     /translation="MNAPVRRLSIVVAILFASLLVSTTLIQYVFAADLNARPDNRRTL
                     LSTYARERGSILVGQTAVASSVPVDDQFKFQREYSNGELYAHVTGSYSFYGASGGLEQ
                     SENAYLSGSSDKLFYRRVSDLFTGRRPQGATLETTLDPAVQRAAVKAMTGIRGAAVAL
                     DPKTGAILAMVSQPTFDPNDLAGHDLSALDENYKKLNTDPQRPLVNRSINGDLYPPGS
                     IFKVITAAAALSSGEYTEDSMLPGPAVLELPGTTASLPNMFPGACGANDQVSFTRAMA
                     ISCNTAFASVGMDIGADALRAQAEKFGFGDSLSVPMRVTPSTIPAELNMPQLAQSSIG
                     QFDVRVTPLQMAMVAAGVANDGAVMKPYLVQSVIGSDLSVIETTDPQELSQAVTPDVA
                     AQLTRMMEAVVTDGSGTRAQIDGVAVAGKSGTAQHGEGRKPHAWFISFAPADDPQIAV
                     AVIAEDGGVTGSEIGGGRVAAPIARQMMEARLK"
     gene            9142..11013
                     /gene="pknB"
                     /locus_tag="PL014_06905"
     CDS             9142..11013
                     /gene="pknB"
                     /locus_tag="PL014_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOT56853.1"
                     /translation="MAEVPKLLGGRYEVDTLLGRGGMAEVHLGYDTRLGRQVAIKMLR
                     SELARDQTFLTRFRREAQSAAGLNHASIVAVYDHGEDSVTESGGAEVRVPYIIMEFVD
                     GKTLREVITERGTLEPTEAMRITEGVLDALAYSHRNGIVHRDIKPANVMIGADGTIKV
                     MDFGIARALADANATMTQTQSVVGTAQYLSPEQAQGQAVDERSDLYSAGCMLFELLTG
                     RAPFMGDSMVSIAYQHVGEQPLPPSRFAPSIPEDLDAVVMHALAKPRDARYQSAGEFR
                     SDLQAVRLGRPISAAARATAVALASGAGAAALTADATEALPAITSEQTQVLSPGAVPP
                     EVPVGTNTETFPALADDTQEKRKKSGWVVLAIFAVAALAALAFGLSSYFGEEEVPQVT
                     VPTVVNKPEQTAIAELARAGLEDDVIRQASDTVPADVVISQTPQGGTEVDRGSTVRLV
                     VSSGPTAITVPDLAGKTADEARQLLGDLGLTLGTITDVDDPKTPQGQIIDSNPAVGAS
                     VSPGSSISVRVGTGQVVVPNVVGKPLSEAQQLLSNVGLAPETSFRVSSEVPENRVLEQ
                     DPPSGKIEIGGTVRIIVAQKAAPTPTPTPTPTPEPTPTPEPSPTQSASPEPEPSPSP"
     gene            complement(11058..11714)
                     /locus_tag="PL014_06910"
     CDS             complement(11058..11714)
                     /locus_tag="PL014_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOT56854.1"
                     /translation="MTRILVVDNYDSFVFTIVGYLEQLGADCEVVRNDAITPADGADF
                     DGVLVSPGPGTPEEAGVSMAMIQACAERAQPMLGVCLGHQALGVVLGATVGRAPELLH
                     GKTSQVHHDGTGVLAGVSDPFTATRYHSLTIAPETMPDSLIANGRTESGIIMAVRHAS
                     LPLHGVQFHPESVLTHGGHRMFANWLAMCGMPDAVERSVGLSPLVHDAAGVARMVAAG
                     "
BASE COUNT         1716 a         4516 c         3916 g         1585 t
ORIGIN      
        1 ggccggggcc cgcccctgcc cgtcacctga gccggctccg cgacggggtc ggccacgcgc
       61 tgaccccttc cccgcgggtg tgatcccgca cgccagttcc aggagacctc atgtccagca
      121 cgacccgttc gatcagccgc gccctcaccc tcggcgcagc agccaccctc gtcttcaccc
      181 tgaccgcctg cggacaggac acgcccaccc caaccggtgc cacggcatcc agctcgtcgt
      241 cgacgacggc agaaaccacc cagggcgcgg gcgccgtggt gctcgacgac ggctgggtca
      301 aggcgaccga cgacgccatg gccggcatgg agccgatgac cgccgcgttc ggcaccctgc
      361 gcaacaccac cgaccacgag gtcaccatca ccggcgggag cagccccgcg gccggcatgg
      421 tcgagctgca cgagacggtg aagaccgaca ccggcgcgat gcagatgcag cccaaggccg
      481 gcggcatcgt catccccgcc ggagggacgc acctgctcga gcccggctcc gaccacctca
      541 tgctcatgaa gctgaccggc cccctcgaga ccggcacgac caccaccctg acgctcagca
      601 cgagcgacgg cgacgtcacc ctcacggtgc cggtgcgggc cttcaccggc gcgcaggagt
      661 cgtacgcacc gagcccgacc cactcatgag cacgcccgac cagggccgag cggcgcagtc
      721 cggccgccgc tcggtcctgc tctcggggct cgccggggcc ggtctcgcgg ccacggcagc
      781 catcgcgacg gcggcatccg ggggcccgtc ggcgagtgcc acctcggcgg ttccaccggg
      841 cacgcgggcg acggggccgg atgccgtggc gcgccccttc cgcggggctc accaggccgg
      901 catcctcgag gctcctccgg cgtacgcgac gttcgtcgcc ctcgatctcg tggacggggc
      961 ggacgccgac gtggtgcggc ggctgctcac cgtgtggacc gacgacatcg agcggttgat
     1021 ggcgggccgc ggcaccctga ccgaccagga gcccgaactc gcctcggtca cagccgagct
     1081 gaccgtgacc gtcggggtcg ggcgacgcgt ggtggaggtc gtcggggcgc aggtgccggc
     1141 ctggttggca ccgctgccgg ccttcgcgat cgaccgcctc cagccccgat ggagtggcgg
     1201 cgacctgctg ctccagatct gcgccacgag ccccacgacg gtggcccacg cccagcggcg
     1261 cctgctcgcg ggagcggccg ggctggcttc ggtgcggtgg gttcagcgcg gcttccgcga
     1321 accgcacgaa gggccggggg tgccgatgcg caacctcttc ggccaggtcg acggcaccgt
     1381 gcagcccgat gtcgccgggc tggacgccga cctgctgtgg agcgccgacg cgggcccgac
     1441 gtggctgcgc ggcggcagct cggtcgtcgt ccggcgcatc gagatggacc tcgacgggtg
     1501 ggaccgcgtc gaccggctct cgcgcgagaa ctcgatcggg cggcggctgg ccacgggcgc
     1561 acccgtgacc gggggtgggc tcgacacggc gcccgacctc gaggccgtcg acgggctcgg
     1621 cttccacgtc gtcgacgacg ctgcgcacat ccgccgggcg cacgccagcg ccccgcacga
     1681 gcggttcctc cgtcggccgt actcgtatga cgatgcgccg gatgccgggt cgacgagttc
     1741 gtcgggtctg gtgttcgtgg cgtaccaggc cgaccccgtg cgccagttcg tgcccgtgca
     1801 ggcccgcctc gccgaactcg acctgctcaa cctctggacc accccgatcg ggtcagcggt
     1861 gttcgccgtg ctgcccggtg ctcaggaggg tgaggtgctg ggccaggccc tgttcacgtg
     1921 acacggcgcg aagggcccac tcccgccggc tgttctgcac gaaatcgggg tcatgacccc
     1981 gatttcgtgc accccgcctg gccggaaaac ggcgaaggac cccccgacaa agcgttgggg
     2041 ggtccttcgt cgtttggtgc gcaagggggg atttgaaccc ccacgccctt tcggacactg
     2101 gcacctgaag ccagcgcgtc tgccgttccg ccacttgcgc gtggtgctca tgcgagcaga
     2161 cacagtgtgc ccgatgaagg gccgatgctc caaagcggcc ccgccattga cggcccaacc
     2221 acgcccgggt ccgcccgtgc gccgaggcga caggagggcc aagagcgcgc tcagccgcgc
     2281 gatcggcccc ccgctgaacg gttgccccga gaccgcagcc gcaccccggg cagcccgggc
     2341 gccgggatcc gcgacacctc accgacataa ccctgcaccg acccgaagcg ccccacggat
     2401 gcccccggca cccccgacgc ctcccactcc tcgcgggccg ccacgacctc gtcgtgcgag
     2461 cgcccgatga agttccacca catgacgacc tcctcggcga acggctcacc cccgagcacc
     2521 accacccgcg cgccgtccga accagcctga agcgcaacgg aacccggatg ccgtggcgca
     2581 cccccggcat ccgacccacc gccccgccca gaaccgtcac caaagtcgcc tgcctcgacc
     2641 accccgagct caccgcgcgc caggcgctgc cctgcgaaca ccaccgagcc agagtcgacg
     2701 agcaccccca gctcgaaccc ggcatccacc ggcacgtcca cgacggcacc gggctccagc
     2761 gcgacctccg ccccgaccag cggcgtctcc accgaaacgg gcgacgtcga cccgaacagc
     2821 gaccccacga acaccgacgc cgcaaccccc ggcgccaccg aaaccacagc aggcacgtgg
     2881 tgctcgaagc gcggctcgca cccggccgac gccgccggaa gcaccaccca cagctgtacg
     2941 ccgtgcaaga cagccgtccc agccgtcgac acctccgagt gcgagatgcc gcgcccggcc
     3001 gtcatgaggt tgacctcccc cgggcggacg gtcgcgaccg tcccgaggga gtcgcggtgc
     3061 tcgacctcgc cggtgaacag ccacgacacc gtcgccagac cacagtgcgg gtgtggcgcg
     3121 acgtccatgc ccccggtgac ctcgacgtca tcgggcccgt agtggtcgac gaagcaccag
     3181 gcgccgacga acgagcgctc cctggcgggc agcgtgcgcc gcaccgtcat cgaccgcagc
     3241 cccccgaggg gcacctcgcg gggcacgatc acctgcacgc cgtcgttcat ccgtccacgg
     3301 tacggggccg cgctgtcgga gatctcccag ccgtgacgga taggctgaca aatcgaccgt
     3361 ccccgaaacc gtgcaggagg tgctccgtgg gtctgttcga tcgcctcgag gccggcatcg
     3421 agcgcgccgt gcagggcacg ttcgccaagc acctgcgctc ggccgtgcac ccggtcgaga
     3481 tcgccagcac gatccgccgc gccatggacg accgcgccgt gagcagctcg ggccgggcca
     3541 tcgtgccgaa cgtcttcacg gtcgagctct cccccggcga ctacgaccgg ctgcaccccg
     3601 acctggccga cgtcgagatg gatctcgtcg ccgccgccga ggagcactgc gaggggcagc
     3661 gctaccagcc cgccggcccg ttcgacatca tcttcgagga gcacgacgac ctcgagaccg
     3721 gggtgttccg catccgcccg tccaaggcgt cgcgaccccg ggcgaccggc accaccggct
     3781 cggagcgcgc ggtgcagcgg ccgagcacgc cggatgccgc tggcccacct cccgctcacg
     3841 tcgcgccgcg cgccgcggtc ccgcccccgt cggctccgcc tgcacccgct gcgccggtcg
     3901 ctcgcgcacc gcgggcaccc gtcgcccgcg agcccgaacc cactccggca ccgccggtcg
     3961 ccgcggctcc gcggcgggtc aacccggccg accgcccctg gctcgacgtc gacggcgagc
     4021 gctacccgct catgggggcc atcacgatcc tcggccgtga cgacgtcgcc gacatcatcc
     4081 tcgacgaccc gggcatctcc cgccggcaca gcgagctccg cgtcacgacc gacggcccgc
     4141 gcttcgtgac gacgatccgc gacctcggct cgacgaacgg cacctacgtc aacggcgagc
     4201 gcatcacgag cgaacacctc caggacggcg accgcatcac cgtgggccgc acgtcgatca
     4261 cgttccgtgg tgggcggagg tgacgtcggc gtgctgagcg agctgaccat caccctcatg
     4321 cggctcggcc tgctcgtgct gctgtggttc ttcgtgttcg ccatcgtcgg cgtcctgcgc
     4381 ggcgacctgt acggcacccg cgtcggccgg cgctcctccg gcagggccaa ggccgcgaag
     4441 ccggccaagc ccgcacccgt tgcggtggtg gccgggcccg cggccggggg cgcggtggcc
     4501 acagccccag cacaggcccc cgctcgaggc gggcgcttcg gccgtcgcgg tggcggcaag
     4561 ggcccgaccc ggctcacggt gacggccggg cagctcgccg gcacgaccct cccgctgcgc
     4621 ccggctggca ccctcatcgg tcgcagtccc gagtgcgccc tcgtgctcga cgacgactac
     4681 gtctcgggcc gccacgcccg catcttccag gccgacgacg gcacgtggca ggtcgaggac
     4741 ctccgatcga ccaacggcac gttcctcggc gcgaaccgcc tgaccgagcc gcgcgaggtc
     4801 gccgtcggca gcgtgctgcg gatcggccag accgtcgtcg aactgcagag gtgacggatg
     4861 ccgttcgcct tccactacgc cgcccgctcg gacgtcggca tggtccggtc caacaacgag
     4921 gactccgcct acgccgggcc gcacctgctc gccctcgccg acggcatggg cggtcacgcg
     4981 ggcggtgacg tcgcgagctc gacggtgatc gccgggctgg tctccctcga cgacgaggcg
     5041 ctcagcgggc gcgacctcag cgaggccctg ctggcccgca tcctgtcagc gaacgccgac
     5101 atcggcaacg aggtgcgctc cgagccgcgc ctggacggca tgggcaccac cctcatcgcg
     5161 atgctgcgct cgggcgacaa gatcgtcctg gcccacatcg gtgactcgcg agccttcatg
     5221 gtgcgcgacg gggtcgtcac ccaggtcacc aaggaccact ccttcgtgca gaccctcgtc
     5281 gacgaggggc ggatcaccgc cgaggaggcc cagacccacc cgcagcgctc gctcgtgacc
     5341 cgggtgctga ccgggtccgt cgacgacgag cccgacctcg tcgtgcgtca gggccgcatc
     5401 ggcgaccgct acctgctggc ctccgacggc ctcaccgact acgtcgcccg cgacaccatc
     5461 gacgaggtgc tgacggcgcc cgggagcccc gctgactgcg ccgaccggct cgtggccctc
     5521 gccctgcgcg ccggcgcccc ggacaacgtg acggtgatcg tcagcgacat cgtcgacggc
     5581 accactgact cggcgccccc gacccagccg caggtcgtcg gcgcggcagc ggttcgcgag
     5641 aagggcaccc ggccgatgcc gacgacgccc gccgcgaagg cggcggcgct cacccaggag
     5701 gcgacgcgag cggcatccgg aagcggtacc gacgagcccg acgccggggg tgacggtgtc
     5761 accctcgccg aggagggtcc gcgctcgcac gggggcacgg ttctgcggct cctcgcgctg
     5821 atcgtcgtgt cggcggtcgt gctcgcgggt gggtcgtatg ccgcgtacgc ctggtcccag
     5881 cagcagtacc acctgagcgg caacgacggc gtcgtcacgg tctaccaagg ggtgaaccag
     5941 acgctggggc cgatctcgtt gtcgcgcccc ctctacacga cgaccatcgc ggtcgacgac
     6001 ctgcctccct cgtaccagag cagcctcgag cagggcatcg acgtcgacga ccgcgccgct
     6061 gccgacgccc gggtcgagga tctgcgcctg caggccacgg cctgccggtg ggcccgcctg
     6121 aacggcgagg agtgccgcac ggtgccgtcg acgtggacgc cgccgagccc cacaccgacc
     6181 ctcacaccga ccccctcccc gagcggtacc cccagcggca cgacccccgc tccgacaccc
     6241 acgaccacat cgcggggccc gtcgcccacc ggcagcccgg tgaccgcatg agtgtcgtct
     6301 cgtcgtacac cccgaggcga ggtcgcaacg tcgagctcgt gctcctcatc gcggccgtcg
     6361 cgatcgtgct gctcgcctac gtcaacgtcg gtgtggcgac gtcggagtcg atgccgccgg
     6421 gcacgatggc cctcgccggt ggctacctcg cgatggccgt cggtttccac ctcgtgctgc
     6481 gctggcgggc gtcctacgcc gacccgctca tgctgccgat catcaccctg ctcaacgggc
     6541 tcgggctggt gatgatccac cgcatcgacc tcgccaacgg cacgtcgatc tccgacgggg
     6601 tggccctgcg ccagctcatg tggaccggtg tcgccgtcgt catcgccgca gccgtgctcg
     6661 tcgtgctgcg cgaccaccgg gtgctgcgcc gctacaccta cctcgcgggc gtggtcggtt
     6721 tcgtcctgct cctgctgccg ctcatgcccg tcatcggcac gtcgatctac ggcgcccgga
     6781 tctggatccg catcggctcg atgtccttcc agcccggcga gatcgccaag atcgcgctgg
     6841 ccatcttctt cgccggctac ctcgtccaga cccgcgacgc cctctcggtc gcggggcgca
     6901 aggtgctcgg gctgaccctg ccgcgcgccc gcgacctcgg accgatcctc atcgcctggc
     6961 tcgccagcct cgccgtcctg gtgttcgagc gcgacctcgg ttcctcgctg ctgttcttcg
     7021 gcctgttcgt cgcgatgctc tacgtcgcga ccgagcgggt gtcctggatc gccatcggcc
     7081 tcaccctgtt cggcgccggc gcctacgtcg cgtacctgat gttcgggcac gtccagaccc
     7141 gcgtgctgct gtggctcgac accttcagcc ccgaggccct gcaggcctcg gaccagctgg
     7201 ccaaggggct catgggcatg gccgccgggg ggatgttcgg cacgggtctg ggtcgggggc
     7261 ggcccgacct gacgtacttc gccgagtccg acttcatcat cccgagcttc ggtgaggaga
     7321 tcgggctcgt cgggctgttc gccctgctca tgctctacgt cctgctcgtc cagcgcgggt
     7381 tgcgcacctc gctcggcacc cgcgacggct tcggcaagct gctcgccgcc ggcctgtcgt
     7441 tctcgctcgc gctccagtgc ttcgtcgtcg tcggcggcgt gacccgggtc atccccctca
     7501 ccggcctgac catgccgttc ctgtcctcgg gcgggtcctc gctgctggcc aactggaccc
     7561 tcgtcgccat cctgctgcgc atctccgacc acgcccgccg ccccatgccg gagatcaacg
     7621 tcccgcacga ggtcggccag gcccacaccg aggtggtgag gatgccgtga acgcgcccgt
     7681 gcgtcgcctc tcgatcgtcg tcgcgatcct cttcgcgtcg ctgctcgtgt cgacgaccct
     7741 catccagtac gtcttcgccg ccgacctgaa cgcccggccg gacaaccggc ggaccctgct
     7801 gtcgacgtac gcccgcgagc gcggctcgat cctcgtcggc cagacggccg tggcgagctc
     7861 ggtgcccgtc gacgaccagt tcaagttcca gcgcgagtac tccaacggcg agctctacgc
     7921 ccacgtcacc ggctcctact ccttctacgg tgcctccggc gggctcgagc agtccgagaa
     7981 cgcctacctg tccgggtcga gcgacaagct gttctaccgc cgggtctccg acctgttcac
     8041 cggccgccgc ccgcagggcg cgaccctgga gacgacgctc gaccccgcgg tccagcgcgc
     8101 ggcagtgaag gcgatgaccg gcatccgagg tgctgccgtc gccctcgacc cgaagaccgg
     8161 cgcgatcctc gcgatggtga gccagccgac gttcgacccc aacgacctgg ccgggcacga
     8221 cctcagcgcg ctcgacgaga actacaagaa gctcaacacc gacccgcagc gcccgctggt
     8281 caaccgcagc atcaacggcg acctctaccc gcccgggtcg atcttcaagg tgatcaccgc
     8341 cgcggcagcc ctgtcgtcgg gggagtacac cgaggacagc atgctgcccg ggcccgccgt
     8401 gctggagctg cccggcacca cggcgagcct gccgaacatg ttcccggggg cctgcggcgc
     8461 caacgaccag gtgtccttca cccgggcgat ggcgatctcg tgcaacaccg ccttcgcctc
     8521 ggtcggcatg gacatcggcg ccgacgcgct gcgggcccag gccgagaagt tcggcttcgg
     8581 cgactccctg agcgtgccca tgcgcgtcac cccgagcacg atccccgccg aactgaacat
     8641 gccccagctc gcgcagtcct cgatcggcca gttcgacgtg cgggtcaccc cgctgcagat
     8701 ggcgatggtc gcggccgggg tcgccaacga cggcgccgtc atgaagccct acctcgtgca
     8761 gtcggtcatc gggtccgacc tgtccgtgat cgagaccacc gatccgcagg agctgtcgca
     8821 ggcggtgacc cccgatgttg cggcacagtt gacccggatg atggaggccg tcgtcaccga
     8881 cggttcgggg acgcgggcgc agatcgacgg ggtggcggtc gcaggcaagt cggggacggc
     8941 gcagcacggt gagggccgca agccccacgc ctggttcatc tccttcgccc cggcggacga
     9001 cccgcagatc gcggtcgccg tcatcgcgga ggacgggggt gtcaccggca gcgaaatcgg
     9061 cggcggccgc gtggccgcac cgatcgcccg ccagatgatg gaagcacggc tgaagtgatg
     9121 aatcgatgag gagcgcacgg catggctgag gtccccaagt tgctcggtgg ccgctacgag
     9181 gtggacaccc tgctcggacg tggagggatg gccgaggtcc acctcggcta cgacacccga
     9241 ctcggtcgcc aggtcgccat caagatgctg cgcagcgagc tcgcgcgcga ccagacgttc
     9301 ctcacgcgct tccgccgcga ggcccagtca gcagctgggc tcaaccacgc atccatcgtc
     9361 gccgtctacg accacggcga ggactccgtc accgagagtg gcggggccga ggtgcgggtg
     9421 ccgtacatca tcatggagtt cgtcgacggc aagacgctgc gcgaggtcat caccgagcgg
     9481 ggcacgctgg agccgaccga ggcgatgcgg atcaccgagg gggtcctcga cgccctcgcg
     9541 tacagccacc gcaacggcat cgtgcaccgc gacatcaagc cggccaacgt catgatcggg
     9601 gcggacggca ccatcaaggt gatggacttc ggcatcgccc gggccctcgc cgacgccaac
     9661 gcgacgatga cccagaccca gtcggtcgtg ggcacggccc agtacctctc gcccgagcag
     9721 gcccaggggc aggcggtcga cgagcgatcg gacctgtact cggcggggtg catgctcttc
     9781 gagctgctga ccggccgggc gcccttcatg ggcgacagca tggtctcgat cgcctaccag
     9841 cacgtcgggg agcagccgct gccgccgtcg cgcttcgccc cgagcatccc cgaggacctc
     9901 gacgcagtgg tcatgcacgc cctggccaag ccccgcgacg cccgctacca gagtgccggt
     9961 gagttccgca gcgaccttca ggcggtgcgc ctcggccgcc cgatcagtgc cgcggcccga
    10021 gccacggcgg tggccctggc cagcggtgcc ggtgcggcgg ccctcaccgc tgacgccacc
    10081 gaggcgctgc cggccatcac gtccgagcag acccaggtgc tgtcacccgg ggccgtgccg
    10141 ccggaggtcc cggtcggcac gaacaccgag accttcccgg ccctggccga cgacacgcag
    10201 gagaagcgga agaagagcgg ctgggtggtg ttggcgatct tcgcggtcgc agcgctggcg
    10261 gcgctcgcgt tcggcctctc gtcgtacttc ggcgaggagg aggtgccgca ggtgacggtg
    10321 ccgaccgtcg tcaacaagcc ggagcagacg gccatcgccg agctggcgcg agccgggctc
    10381 gaggacgacg tcatccgcca ggccagcgac acggtgccgg ctgacgtcgt gatcagtcag
    10441 acgccccagg gtgggaccga ggtcgatcgc ggcagcaccg tgcgcctcgt cgtgtcctcc
    10501 ggcccgaccg cgatcaccgt gcccgacctc gccggcaaga ccgctgacga ggcccgtcag
    10561 ctgctcggcg acctcggcct gaccctcggc accatcaccg acgtggacga ccccaagacc
    10621 ccgcaggggc agatcatcga ctccaacccg gcagtcggcg cctcggtgag ccccgggtcg
    10681 tcgatcagcg tgcgggtcgg aaccggtcag gtcgtcgtcc ccaacgtcgt cggcaaaccg
    10741 ttgagcgagg cccagcagct gctctcgaac gtcggcctgg ctcccgagac gtcgttccgc
    10801 gtcagctcgg aggttccgga gaaccgggtg ctcgagcagg atccgccgag cggcaagatc
    10861 gagatcggtg gcacggtgcg gatcatcgtc gcgcagaagg cggctccgac gccgaccccc
    10921 acgccgaccc ccacgccgga accgacgccg accccggagc cgagccccac gcagtcggca
    10981 tcacccgagc ccgagccgtc cccctccccc tgaccgcagg gtgcacctga ctccgcgagg
    11041 aggcggtcgg atgccggtca gccggcggcg accatccgcg ccacacccgc ggcgtcgtgc
    11101 accagcgggc tgagcccgac ggagcgttcg acggcatccg gcatcccgca catcgccaac
    11161 cagttcgcga acatccggtg gccgccgtgg gtgagcaccg actcggggtg gaactgcacc
    11221 ccgtgcagcg gcaggctcgc gtgacgcacg gccatgatga tccccgactc ggtgcgcccg
    11281 ttggcgatca gcgagtcggg catcgtctcg ggggcgatgg tcagggagtg gtagcgcgtc
    11341 gcggtgaagg ggtccgatac tccggccagc accccggtgc cgtcgtggtg cacctgcgag
    11401 gtcttgccgt gcagcagttc cggcgcgcgg cccaccgtcg ccccgagcac gaccccgagc
    11461 gcctggtggc ccaggcagac accgagcatc ggctgggcgc gctccgcgca ggcctggatc
    11521 atcgccatcg agaccccggc ctcctcgggc gtccccgggc cgggcgagac gagcaccccg
    11581 tcgaagtcgg cgccgtcggc cggggtgatc gcgtcgttgc gcacgacctc gcagtcggcg
    11641 ccgagctgct cgaggtagcc gacgatcgtg aagacgaagc tgtcgtagtt gtcgacgacg
    11701 aggatccggg tcatgggtcc atggtgcacc gtc
//
LOCUS       DAOSOC010000070        11718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5447_length_11718_cov_9.647261, whole genome shotgun sequence.
ACCESSION   DAOSOC010000070 DAOSOC010000000
VERSION     DAOSOC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11718
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5447_length_11718_cov_9.647261"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1073
                     /locus_tag="PL014_06915"
     CDS             <1..>1073
                     /locus_tag="PL014_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56855.1"
                     /translation="HQPLSALGEQAVGEVLDRVDDDTHAVGSMLRRQVWDALRTVFSA
                     VPMWIVAGLTWWPAWILFPVAGAAAFAIIRRLLPAIAERTVVEEAAWTDHAAALEEGV
                     SARDDLRTSLGQAHVVQRLSTLSATVHRRFDELLALQARATRRTGLLLYSLLAGTSIA
                     GVALVISDDLSVARLVTLFLVTSTFVGQIDQMARHLPELQTGIGAITRLRQLLESPPE
                     PEGGRPVPAGNVGVEFRDLHFSYAEGDFALDGVSLDIPAGRTCALVGRSGSGKSTLAA
                     LLSRATDPPRGAVFLGGIDVCDLDLHALRSAVGVVTQRTDILAGTLAENIALFADVPT
                     STVRAAVTELGLDDWVDGLPDGL"
     assembly_gap    1074..1173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1174..2633
                     /locus_tag="PL014_06920"
     CDS             <1174..2633
                     /locus_tag="PL014_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56856.1"
                     /translation="TAAIRRYPRWWVEVLLRVRMSILIGQTRQHRLESTPPGEVVARA
                     MDADRYVLYADRWVDFLNGLAVVAVTAILGGSALAGGVLLGVLVGSALASAVGRPIAG
                     RSATESSAARATFGRSLVSVLESARTIKLASATPAARSHLRRVDSGRVDAAVFEHRVQ
                     AILDGVPVIMVQIGVVAAWAAFASGRWSLATAILVSGAVNGFDWFGRVAGAVVTEAPG
                     TRSWQKATSALAGGVDLVALPPGIDLVEGTAPAAPTPQHVPLRELELRDVGAIHDDGT
                     RGVEGVDLTVRAGELVLLLGQVGSGKSSLLRGLAGLIHHTGSITWNGVEVHDAQVFLR
                     PGQVSYVSQVPRVLSGSFADNVVLGHGRALDRAIADARLREDVEDAGGHDALVGHRGV
                     RLSGGQVQRLALARALATEAEVLLADDVSSALDATTEIDLWSALRARGTTVIGATSKR
                     AALGRADRVVVLVDGRVAEVGPWSALEQRWAHLAG"
     gene            2701..4245
                     /gene="ilvA"
                     /locus_tag="PL014_06925"
     CDS             2701..4245
                     /gene="ilvA"
                     /locus_tag="PL014_06925"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HOT56857.1"
                     /translation="MPHTRPLTDAEATAYLKRILTSRVYDVVDETPLDAAPGLSARLG
                     STVLLKREDLQSVFSFKIRGAYNTMAKLSPQARAAGVIAASAGNHAQGVARAASVLGC
                     HAVIVMPTTTPQLKVDAVRAAGVEVVLHGDSYSDAGAHAAALAHERGLTMVHPFDDPE
                     VIAGQGTIGMEILRQVTGPLDAVFVAVGGGGLISGIAAYVKALRPDVKVIGVQTDDSD
                     AMARSLEAGHRVELEHVGLFSDGTAVRLVGEETFRVASALVDEMIRVDTDAVCAAIKD
                     VFTDTRAILEPAGALAVAGLKEYAVRPGAEGGTYVAVACGANMDFDRLRFVAERAEIG
                     EQREAVFAVTIPEQRGSFRRLLGVIGPGREVTEFNYRIDDSDTAHVFVGLQVHGREEA
                     RRLARGFEDAGLPALDLTDDELAKLHLRHLVGGRTTLAHDEVLHRYEFPERPGALVQF
                     LEAMAPAWNISLFHYRGHGSDVGRVLVGMQVPPSDREAFDTFRDTLGYRSWDETDNPA
                     YRLFLR"
     gene            complement(4283..7051)
                     /locus_tag="PL014_06930"
     CDS             complement(4283..7051)
                     /locus_tag="PL014_06930"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOT56858.1"
                     /translation="MHTVVARLRRQFGAELIAREHAGYLVPDAVRTDVDEVLLHRSQA
                     KACAAQSDWSGRREADRAALGLWAGPVALEGVRDDLVLVERARLGDLRRALLDDLAVS
                     LLDDPGGDPDEAHDLALELMAGDPLDEGAAALGMRSAQRLGRQAEALSIYTRIRRDLR
                     EELGIDPGRALTELHGRVLAQAPDQVPAHEPLGGPAVPRDVVLHPARILPAPRTPTVG
                     RRADLESALGALSAGHRLITVTGPAGVGKSRLLAEIGAALRLSEPDIYHVVLEPHAEL
                     SAGALALVVGSMSGLPMDGKDPVRQLAESLAHEQAVILVDEAEWALSGAAEIARALLD
                     RCPGIRLVITSRTPLGILGERLVVLDSLTVPAPGASREEVCSAAAVQLLASRLADRGT
                     ERAADLVAWPEQDLQTLAEVARRLDGLPLALELAAGRFPDGDLDRLLDLVAAPLDLVA
                     DEVDREPRHQSLREAIGWSIDRLSPGERRALARLSVFVGTFTRGAALAVIASPEADVQ
                     LRALVRGNLVHAAHTARGQGLRLLTTVRDAAREVLGDLGEHESAQRRHREWYAARWRG
                     AQLSDTLIEDVGLAQEDHLAALDGALSAGDTGSAVDLGLALFRRWQFTEAVATASPWM
                     DQLSRMPGLTDAQSARLAVARTAISHVRQWEDTDVPGMMQSLRADPDWSIMLCITESI
                     AAYTAGEHTRAVAMAELALDASRTGAPHHLPESLAVMAVVDGVAGNHEAATAHVEEAR
                     ARIGPHPSAVHLVTVVPKLALAMLDAGRPDVALDLLTRAADGAQSRFGIQPTTTTVVN
                     LGWAALGAGRPEEAFGWFTRGLHREAAWSPGFAEAAAGVGCAGVATRWEHGPAVLLAA
                     DRLLATSAIQLSGSLAEHVESARGDVHWPPDTPLSTLPVDVVAHRVLQLAAGHAAALS
                     SEPENASV"
     gene            complement(7003..7752)
                     /locus_tag="PL014_06935"
     CDS             complement(7003..7752)
                     /locus_tag="PL014_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT56859.1"
                     /translation="MTTNTAGLPGIHVHIRTLAAGDHDTLTTIMSGMSLRSRVARYHT
                     GLPHLTPRMLRHLTDVAPGRHVALVAEQDGAPVGIVRWVREDETTAEIALEVVDAAHG
                     RGVGRALLAAARASAVEAGIERLVAYVTADNRPVHRWLSRHAAHREDDRFVIALPEKL
                     AEPNRALRGSMDECAPASWGHSVSRPPTVRNCPPAADHATSSRPSCSGEGAPSRLMSF
                     SSSSGPSRQPASTSPPCTLSSHASGVSSGPS"
     gene            complement(7800..8630)
                     /locus_tag="PL014_06940"
     CDS             complement(7800..8630)
                     /locus_tag="PL014_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT56860.1"
                     /translation="MTKPDTPLDDVERAHLVDPTDTTYAIDRFAPSPDVVDLVRRHWV
                     PMWHLPEGESSEQAVLQYPVCLVVVAHDYARFYGVQSGLSRTTLVGTGWAAGVMLQPG
                     AGRLLLGQDVSTVTDRHVELSDVLGSQVVTDVRAAMTDPQDPGCRREAADSLEAVIRA
                     HLPLDDEGRLVNDLVDHIEHDPSVRQVSQVCDRFGLSERALQRLTRRRLGLTPKWLIQ
                     RRRLHEASERLRAGPGDLAAIAHDLGYADQAHFTRDFRRVTGITPGGFARRFAAGADA
                     "
     gene            8664..9185
                     /locus_tag="PL014_06945"
     CDS             8664..9185
                     /locus_tag="PL014_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT56861.1"
                     /translation="MNPTYATLTDRFTDVVDAVPNWLAASPCEGWSVGDVVDHVVSTQ
                     RDFLRAHTDLAPEPTTADPAQRWAAHRALVAQVLPGLADVAYETPFGPSTVGQTMTTF
                     YGFDLIVHRWDIATGAGLVAPLGDEEIDILEAVVPTFGEHLYAEGICKGPLDVGPDAD
                     RTARILAVLGRAA"
     gene            complement(9187..9984)
                     /locus_tag="PL014_06950"
     CDS             complement(9187..9984)
                     /locus_tag="PL014_06950"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOT56862.1"
                     /translation="MSQRIGGHVDSSDPVAEALARGATLSQIFLGDPQSYKGPVVAYA
                     GGAPALKAAAEDAGVDLYVHAPYPINVASTNNRVRIPGRKLLQQHLHAAAAIGAKAVV
                     VHGGHLGKDEDAAVGFDNWRKCIDGLDLPVPLLIENTAGGDNAMARRLEAIARLWDAI
                     GAASGGDQVGFCLDTCHAHAGGNDLGDVVERVLTITGRIDLVHANDSRDTFDSGADRH
                     TNFGSGLIDAGDLADVVRAAAAPVVCETPGGVEGQAADIAWLRARLG"
     gene            10028..10789
                     /locus_tag="PL014_06955"
     CDS             10028..10789
                     /locus_tag="PL014_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT56863.1"
                     /translation="MLTANTTAIVTGGASGLGAATAAALAAAGSRVIALDLPAACESG
                     PAVEGVQYVPTDVTSEQEVRAAVATASDDPAKPLRIVVNCAGIGPSTRILGRKGVHDL
                     ALFRKVVEINLVGSFTVMALAAEAIAQTEPDADGQRGVVINTASIAAFDGQVGQAAYS
                     SSKGGIVGLTLPAARDLAQYGIRVVTIAPGIVETPMLATVSDEFRAGLAAGVPFPQRL
                     ARPEEYAKLALAIVDHDYLNGETIRMDGALRMAPR"
     gene            10825..11616
                     /locus_tag="PL014_06960"
     CDS             10825..11616
                     /locus_tag="PL014_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOT56864.1"
                     /translation="MPGRHLRSSRTLRDDLHQEIALPGDVGRIVSLVPSLTEAVAVTV
                     PDRLVGATDWCTHPADLDARGVGRVRGTKNPDLAAIAALQPDLVVANKEENRELDVRR
                     LRERGIPVWVTDIDTVPASITSMRRLLRDVLGLGEDPAWLTEADRAWSGPVPAPSARV
                     VVPIWRDPWMVIGRPTFTADLLARSGFEVVTAGVTGRYPGLEPEVIDDPALADLVLLP
                     DEPYVFTAADGPEVFERLPTRLVSGRLITWYGPSLIEARISLDRS"
BASE COUNT         1545 a         4232 c         4186 g         1655 t
ORIGIN      
        1 caccaaccgc tgtcggcgct cggtgagcag gcggtggggg aggtgctcga ccgggtcgat
       61 gacgacaccc acgccgtggg ctcgatgctg cgccgccagg tgtgggacgc cctgcgcacc
      121 gtgttctccg ccgtccccat gtggatcgtc gctggtctga cctggtggcc ggcctggatc
      181 ctcttcccgg tcgctggggc ggccgcgttc gccatcatcc gcaggctgct cccggccatc
      241 gcggagcgga ccgtcgtcga agaggccgcc tggaccgacc atgccgcagc cctcgaggag
      301 ggggtctccg cccgagacga cctgcgcacc agcctgggcc aggcccacgt cgtgcagcgc
      361 ctcagcaccc tgtcggcaac cgtgcaccgc cggttcgacg agctgctggc gctgcaggcc
      421 cgcgccaccc ggcgcaccgg cctcctgctc tactcgttgc tcgcgggcac gagcatcgca
      481 ggggtggcac tggtcatcag cgacgacctg tcggtggccc gcctggtcac cctgttcctc
      541 gtcacgagca cgttcgtcgg gcagatcgac cagatggccc gccacctgcc cgagctgcag
      601 accggcatcg gggccatcac gcgcctgcgg cagctgctcg agtcgccacc tgagcccgag
      661 ggcggtcggc ctgttcctgc cgggaacgtc ggggtcgagt tccgggacct gcacttctcc
      721 tacgccgagg gggacttcgc cctggacggc gtcagcctcg acatccctgc gggccggacg
      781 tgcgccctcg tcggacgatc cgggtcgggc aagtccacgt tggccgccct gctgtcgcgc
      841 gccaccgacc cgccacgggg ggcggtcttc ctcggtggca tcgacgtgtg cgacctggac
      901 ctccacgcgc tgcgttctgc cgtcggcgtg gtcacccagc gcaccgacat cctcgccggg
      961 accctcgcgg agaacatcgc cctcttcgcc gacgtgccca cctcgacggt gcgagcagcc
     1021 gtcaccgaac tcggcctcga cgactgggtc gacggactgc ccgacggcct cgannnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgaccgc ggccatccgg cgctacccgc
     1201 gctggtgggt cgaggtgctg ctgcgggtgc gcatgtcgat cttgatcggg cagacgcgac
     1261 agcaccggct cgagagcacg ccgcccggtg aggtcgtggc ccgcgcgatg gacgcggacc
     1321 gctacgtcct ctacgccgac cgctgggtcg acttcctcaa cgggctcgcc gtcgtcgccg
     1381 tcaccgcgat cctcggcggc agtgcactgg ccggaggtgt gctgctcggg gtgctcgtcg
     1441 gttcggccct cgcctcggcc gtcgggcggc ccatcgcggg gcgctcggcc accgaatcct
     1501 ccgcggcccg ggccaccttc ggccgctccc tggtgtccgt cctcgaatcc gcgcgcacca
     1561 tcaagctggc ctcggccacc cccgctgccc gcagccacct gcggcgggtc gacagcggcc
     1621 gggtcgacgc ggccgtcttc gagcaccggg tgcaggccat cctcgacggc gtgcccgtga
     1681 tcatggtgca gatcggggtg gtcgctgcct gggcggcgtt cgcctccggc cgctggagcc
     1741 tggccaccgc gatcctcgtc tcgggtgccg tcaacgggtt cgactggttc ggccgggtcg
     1801 ccggagccgt cgtcaccgag gcgccgggaa cccggtcctg gcagaaggcg acgagcgccc
     1861 tcgccggggg tgtcgacctc gtcgccctcc cgccgggcat cgacctcgtc gagggcaccg
     1921 cacccgcagc gcccaccccg cagcacgtcc cgctgcgcga gctcgaactg cgcgacgtcg
     1981 gcgccatcca cgacgacggc acccgcggcg tcgagggggt cgacctcacg gtccgcgccg
     2041 gcgaactcgt gctgctcctc gggcaggtcg ggtcgggcaa gtcgagcctg ttgcgcggcc
     2101 tcgccggcct gatccaccac accggctcga tcacgtggaa cggggtcgag gtccacgacg
     2161 cgcaggtctt cctgcggccg ggccaggtgt cgtacgtctc gcaggtgccg agggtgttgt
     2221 caggctcgtt cgccgacaac gtcgtgctcg gtcacggccg cgccctcgat cgggccatcg
     2281 ccgacgcccg gctgcgcgag gacgtcgagg atgccggggg gcacgacgcg ctcgtcggtc
     2341 accgcggcgt gcggttgtcc gggggtcagg tccagcggct ggcactggcc cgggcgctgg
     2401 ccaccgaggc cgaggtgttg ctggccgatg acgtgtcgag cgccctggac gccaccaccg
     2461 agatcgacct gtggtctgcc ctgcgcgccc gtggcaccac cgtcatcggg gccacgtcga
     2521 agcgggccgc gctcggccgg gccgaccggg tcgtcgtgct cgtcgacggc cgggtggccg
     2581 aggtcgggcc gtggtcggcg ctggaacagc gctgggccca cctcgcgggg tgaggcggac
     2641 ggcatccgtc caccatggtc gggccgtccc ggaaccgggc gccgcggcta cccttgcgcc
     2701 gtgccgcaca cccgacccct gaccgacgcc gaggccacgg catacctcaa gcggatcctc
     2761 acctcgcgcg tctacgacgt cgtcgacgag accccgctcg atgccgcacc cggcctcagc
     2821 gcccgcctcg gcagcacggt gctgctcaag cgcgaggacc tccagtcggt gttctcgttc
     2881 aagatccgtg gcgcctacaa cacgatggcg aagctcagcc cacaggcccg cgccgccggg
     2941 gtcatcgcgg cgtccgccgg caaccacgcg cagggggtcg cccgcgcggc atccgtgctc
     3001 ggctgccacg ccgtcatcgt catgccgacg accacaccac agctcaaggt ggacgccgtg
     3061 cgcgccgccg gtgtcgaggt cgtcctgcac ggcgactcct actccgacgc cggtgcccac
     3121 gccgcagccc tcgcccacga gcgcggcctg acgatggtgc accccttcga cgacccggag
     3181 gtcatcgccg ggcagggcac catcggcatg gagatcctgc gccaggtcac cggccccctg
     3241 gatgccgtgt tcgtcgccgt cggcggtggt gggctcatct ccggcatcgc ggcctacgtc
     3301 aaggcactgc gccccgacgt caaggtcatc ggggtgcaga ccgacgactc cgacgccatg
     3361 gcccgctcac tggaggccgg gcaccgggtc gagctcgagc acgtggggct gttctcggac
     3421 ggcaccgcgg tccggctcgt cggtgaggag accttccggg ttgcgagcgc cttggtcgac
     3481 gagatgatcc gggtcgacac ggatgccgtg tgcgcggcga tcaaggacgt cttcaccgac
     3541 actcgggcca tcctcgagcc ggcaggtgcc ctcgccgtcg cagggctcaa ggagtatgcc
     3601 gtgcgtccgg gagccgaggg cggcacctac gtcgcggtgg cctgtggcgc caacatggac
     3661 ttcgaccgct tgcgcttcgt cgccgaacgg gccgagatcg gcgaacagcg cgaggccgtg
     3721 ttcgcggtga ccatcccgga gcagcggggc agcttccgca ggctgctcgg cgtcatcggc
     3781 ccgggccgtg aggtcaccga gttcaactac cgcatcgacg acagcgacac cgcccacgtc
     3841 ttcgtggggt tgcaggtcca cggtcgcgag gaggcccgcc ggctcgcgcg ggggttcgag
     3901 gacgccggtc tgcctgccct cgacctcacc gacgacgagc tcgccaagct gcacctgcgc
     3961 cacctcgtgg gtggccgcac gacccttgcc cacgacgagg tgctccaccg ctacgagttc
     4021 cccgagcggc ccggtgcgct cgtgcagttc ctcgaggcga tggcgccggc atggaacatc
     4081 tcgctattcc actaccgggg gcacggctca gacgtcggtc gcgtgctcgt cggcatgcag
     4141 gtgcccccga gtgaccgcga ggcgttcgac accttccgcg acaccctggg ctaccggtcg
     4201 tgggacgaga ccgacaaccc ggcctaccgg ctgttcctgc gctgaggctg ctcctgccct
     4261 gaggcaggct cggtcgcccc ggctacaccg aggcgttctc gggctccgag ctcagcgccg
     4321 ccgcgtggcc ggccgcgagc tgcagcaccc ggtgcgcgac gacatcgacg ggcagggtcg
     4381 acaggggcgt gtcggggggc cagtgcacgt caccgcgtgc gctctccacg tgctcggcca
     4441 ggctccccga cagctggatc gccgacgtcg cgagcaggcg gtcggcggcg aggagcaccg
     4501 ctgggccgtg ctcccagcgc gtcgcgacac cggcgcaccc gacgccggcc gcagcctccg
     4561 cgaagcccgg gctccacgca gcctcccggt gcagccctcg ggtgaaccag ccgaacgcct
     4621 cctctggtct tcccgcgccc agggccgccc accccaggtt gaccacggtc gtcgtcgtgg
     4681 gctggatgcc gaagcgcgac tgcgctccat ccgcagcgcg cgtcagcagg tcgagcgcga
     4741 cgtccgggcg cccggcatcc agcatggcga gggcgagctt ggggacgacc gtcacgagat
     4801 gcaccgccga ggggtgcggc ccgatccggg cgcgagcctc ctcgacgtgc gcggtggctg
     4861 cctcgtggtt cccggcgacg ccgtccacca ccgccatgac ggccagcgac tcgggaaggt
     4921 gatgcggggc cccggtccga gacgcgtcca gggccagctc ggccatggcc acggcgcggg
     4981 tgtgctcccc agccgtgtac gcagcgatgg actcggtgat gcacaacatg atcgaccagt
     5041 ccgggtcagc gcgcagcgac tgcatcatgc cggggacgtc ggtgtcctcc cactggcgca
     5101 cgtgcgagat cgcggtacgc gccacggcga gccgggcgct ctgggcgtcg gtgagccccg
     5161 gcatccgcga gagctggtcc atccagggcg aagcggtcgc gaccgcctcg gtgaactgcc
     5221 agcggcggaa cagcgccagg ccgaggtcga cggccgaccc ggtgtcaccg gcggacaggg
     5281 caccgtcgag cgcggcgagg tggtcctcct gggccaggcc gacgtcctcg atgagggtgt
     5341 cgctcaactg cgccccacgc cagcgtgccg cgtaccactc ccggtgccgt cgctgagcgc
     5401 tctcgtgttc gccgaggtcg ccgagcacct cccgggcggc atcgcgcacc gtggtcagca
     5461 ggcgcaggcc ctgacctctg gccgtgtgcg cagcgtgcac gaggttgccc cggacgaggg
     5521 cacgcagctg cacgtcggcc tcgggggagg cgatcaccgc gagtgccgct ccccgcgtga
     5581 acgtcccgac gaacaccgac agcctcgcca gcgcccggcg ctcaccgggt gacagccggt
     5641 cgatgctcca gccgatggcc tcacgcagcg actggtggcg cggctcgcgg tccacctcgt
     5701 cggccacgag gtccaacggt gcggccacga ggtcgagcaa cctgtcgagg tcgccgtcgg
     5761 ggaagcgccc ggcggccagc tccagggcga gggggaggcc atcgagccgc cgcgcgacct
     5821 cggccaaggt ctgcaggtcc tgctccggcc acgccacgag gtcggcggca cgctcggtcc
     5881 cgcggtcggc aagccgggag gccagcagct gcacggccgc cgccgagcag acctcctcgc
     5941 gtgaggcgcc gggcgcgggg acggtcaacg agtcgagcac gacgaggcgc tccccgagga
     6001 tcccgagcgg cgtgcgcgag gtgatcacga gccggatgcc ggggcaccgg tcgagcagcg
     6061 ccctcgcgat ctcggccgct cccgacaggg cccactcggc ctcgtcgacg aggatgacgg
     6121 cctgctcgtg cgcgagcgac tccgcgagct ggcggaccgg gtccttgccg tccatcggca
     6181 acccgctcat cgacccgacg acgagcgcga gcgccccggc ggacagctcg gcgtggggct
     6241 cgagcacgac gtggtagatg tccggttcac tcaggcgcag cgccgccccg atctcggcga
     6301 gcaggcgcga cttgccgacc ccggccggtc cggtcaccgt gatgagccgg tggcccgcgg
     6361 agagcgcacc gagagccgac tcgaggtccg cgcggcggcc gaccgtcggg gtgcggggcg
     6421 ccggcaggat gcgcgcgggg tgcagcacga cgtcacgggg cacggctggt cccccgaggg
     6481 gctcgtgggc cgggacctgg tccggggcct gtgcgagcac tcggccgtgc agctcggtca
     6541 gggcgcgacc ggggtcgatg cccagctcct cgcgcaggtc tcggcggatc ctcgtgtaga
     6601 tcgacaaggc ctcggcctgt cggcccagcc gctgggccga gcgcatgccc agggccgcgg
     6661 ctccctcgtc gagggggtca ccggccatga gctcgagggc gaggtcgtga gcctcgtccg
     6721 ggtcgccgcc cgggtcgtcg agcagcgaga ccgcgaggtc gtcgagcagg gcccgacgca
     6781 ggtcgccgag ccgcgcccgt tcgacgagga cgaggtcgtc gcgcactccc tcgagggcca
     6841 ccggtccggc ccacagtccc agggctgccc ggtcggcctc tcggcgcccc gaccagtcgc
     6901 tctgcgcggc acaggccttg gcctgtgagc gatgcagcag gacctcgtcg acatcggttc
     6961 gcaccgcgtc gggcaccagg tagccggcgt gttcgcgagc aatcagctcg gccccgaact
     7021 gacgccggag gcgtgcgacg acagtgtgca cggcggcgac gtcgaggctg gctgcctgct
     7081 cggaccagac gagctcgaga aggacatcag ccgggacggg gcgccctcgc cggagcagga
     7141 gggccgcgag gaggtcgcgt ggtcggccgc cggagggcag ttgcgaaccg tcggcggtct
     7201 cgacacggag tggccccagg aggctggcgc gcattcgtcc atcgaaccac gaagagcacg
     7261 gttcggctca gcgagttttt ccggcagcgc gatgacgaac cggtcgtcct cgcggtgcgc
     7321 cgcgtgacgg ctgagccacc ggtgcacggg gcggttgtcc gcggtgacgt aggcgaccaa
     7381 ccgctcgatc cctgcctcga cggcgctcgc ccgcgcggcg gcgagcagcg cccgaccgac
     7441 gccgcgtccg tgggccgcgt ccacgacctc gagggcgatc tcggccgtcg tctcgtcctc
     7501 gcgcacccag cgcacgatgc cgaccggggc gccgtcctgc tccgcgacga gggcaacgtg
     7561 tcgccccggt gcgacgtcgg tgaggtggcg cagcatccgc ggagtcaggt ggggcagccc
     7621 ggtgtggtag cgggcgacgc gcgagcgcag cgacatcccg gacatgatcg tggtgagggt
     7681 gtcgtggtcc ccggcggcca gggttcggat gtgcacgtgg atgccgggca gcccggcagt
     7741 gttcgtcgtc atggctccac cgtgcggtgg gcccctgacg tcgccctgtc accgacctgt
     7801 caggcatcgg cgccggcagc gaaccggcgc gcgaaaccgc ccggcgttat ccctgtcacg
     7861 cgccggaagt cccgggtgaa gtgcgcctgg tcggcgtacc cgaggtcgtg ggcgatggcg
     7921 gcgaggtctc ctgggccggc gcgcagccgc tcactggcct cgtggagccg acgacgctgg
     7981 atgagccact tcggcgtcag tccgagccgt cgtcgggtga gccgctggag tgcgcgctcg
     8041 ctgagcccga accggtcgca cacctgcgag acctgccgga ccgaggggtc gtgctcgatg
     8101 tggtcgacga ggtcgttgac gagccgtcct tcgtcgtcga gcgggaggtg ggcgcggatc
     8161 acggcctcga ggctgtcggc cgcctcgcga cggcatccgg ggtcctgcgg gtcggtcatg
     8221 gcggcgcgga cgtccgtgac gacctgactg ccgaggacgt ccgagagctc gacgtgcctg
     8281 tcggtcacgg tcgacacgtc ctggcccagg agcagccgtc cggcgccggg ctgcagcatg
     8341 accccagcgg cccagcccgt gccgaccagc gtggttcgcg agagtcccga ctggacgccg
     8401 tagaagcgtg cgtagtcgtg ggcgacgacc accaggcaca cggggtactg caggaccgcc
     8461 tgctccgagg actcgccctc ggggaggtgc cacatcggca cccagtgccg acgcacgaga
     8521 tcgacgacgt cgggggaggg cgcgaaccgg tcgatcgcgt aggtggtgtc ggtcgggtcg
     8581 acgaggtgcg cgcgttcgac gtcgtcgagc ggtgtgtcgg gtttggtcaa gtgccggccc
     8641 gcccgcgctc gtaccgtcgc agcatgaacc caacgtatgc caccttgacc gaccgcttca
     8701 ccgacgtcgt ggatgccgtg ccgaactggt tggccgcctc accgtgcgag ggctggagcg
     8761 tcggcgacgt cgtggaccac gtcgtgagca cgcagcgtga cttcctgcgg gcgcacaccg
     8821 accttgctcc cgagccgacg actgcggacc cggcacagcg gtgggcggct caccgggccc
     8881 tcgtggcgca ggtgctgccc ggcctcgccg acgtcgccta cgagaccccc ttcgggccga
     8941 gcaccgtcgg gcagacgatg accacgttct acggcttcga cctcatcgtc caccggtggg
     9001 acatcgcgac cggtgccggg ctggtggccc ctctcggtga cgaggagatc gacatcctcg
     9061 aagcggtcgt gccgacgttc ggcgagcacc tgtacgcgga gggaatctgc aagggccccc
     9121 tggacgtcgg gccggacgcc gaccggacgg ccaggatcct cgcggtgctc ggccgggcgg
     9181 cctgactcag ccgaggcgcg cgcgaagcca ggcgatgtcg gcagcctgac cctcgacgcc
     9241 cccgggcgtc tcgcagacca ccggcgcagc tgccgcccgc acgacgtcgg cgaggtcacc
     9301 cgcatcgatg agccctgacc cgaagttcgt gtgccggtcg gcgcccgagt cgaaggtgtc
     9361 gcgcgagtcg ttggcgtgca cgaggtcgat gcgacccgtg atggtcagca cgcgctcgac
     9421 gacgtcaccg aggtcgttac cgccggcgtg ggcgtggcag gtgtcgaggc agaacccgac
     9481 ctggtcaccc ccggatgccg cgccgatggc atcccacaga cgggcgatcg cctcgagccg
     9541 gcgggccatg gcgttgtccc cacccgcggt gttctcgatg agcagcggca ccggcaggtc
     9601 gaggccgtcg atgcacttgc gccagttgtc gaacccgacc gcagcgtcct cgtccttgcc
     9661 gaggtggccg ccgtgcacga cgaccgcctt ggcgccgatc gcggcggcgg cgtgcaggtg
     9721 ctgctggagg agcttgcggc ccgggatccg cacccggttg ttcgtcgacg cgacgttgat
     9781 cgggtagggc gcgtggacgt acaggtcgac cccggcatcc tcggccgcag ccttgagggc
     9841 aggggcaccc ccggcgtacg ccacgaccgg tcccttgtag ctctgcgggt cgccgaggaa
     9901 gatctgcgag agggtcgccc cacgggcgag tgcctccgcg acggggtcgc tgctgtcgac
     9961 gtgcccaccg atgcgttgtg acatggaacc accgtagggg gcgccaccga ctggcaggat
    10021 ggcgaccatg ctgaccgcga acaccaccgc catcgtgacc ggaggagcat cagggctcgg
    10081 agccgccact gcggccgccc tcgccgccgc cgggtcccgc gtcatcgccc tcgacctgcc
    10141 cgcggcgtgc gagtcgggcc cggcggttga gggggtgcag tacgtgccca ccgacgtgac
    10201 gtccgagcag gaggtccgcg cggctgtggc cacggcatcc gatgatccgg cgaagccgct
    10261 gcgcatcgtc gtcaactgcg ccggcatcgg cccgtcaacc cgcatcctcg ggcgcaaggg
    10321 cgtgcacgac ctcgccctgt tccgcaaggt cgtcgagatc aacctcgtcg gctcgttcac
    10381 cgtcatggcg ctggctgccg aggccatcgc ccagaccgag cccgacgccg acgggcagcg
    10441 cggtgtcgtc atcaacaccg cgagcatcgc cgccttcgac ggtcaggtcg gccaggccgc
    10501 ctacagctcg tccaagggcg gcatcgtcgg gctcacgctc ccggccgctc gcgacctcgc
    10561 ccagtacggc atccgggtcg tcaccatcgc ccccggcatc gtcgagaccc cgatgctcgc
    10621 gaccgtgtcc gacgagttcc gggcggggtt ggccgccggg gtgcccttcc cgcaacgcct
    10681 ggcgcgtccc gaggagtacg ccaagctggc gctcgccatc gtcgaccacg actacctcaa
    10741 cggcgagacg atccgcatgg acggcgcgct gcggatggcc ccgcgctgac gcgcacgtca
    10801 cggatgccgg gtcgcgaggg tcggatgccg ggtcgtcacc tgcgctcgtc gcgcaccctg
    10861 cgggacgatc tgcaccagga gatcgcgttg cccggtgacg tggggcgcat cgtctccctc
    10921 gtgccgtcac tgaccgaggc cgttgccgtc accgtgcccg accggctcgt cggtgccacc
    10981 gactggtgca cccatcccgc tgacctcgac gcccgcgggg tcgggagggt gcgtggcacg
    11041 aagaacccgg atctcgcggc catcgccgcc ctgcagcccg acctcgtcgt ggccaacaag
    11101 gaggagaacc gcgaactgga cgtgcgccgg ttgcgtgagc gcggcatccc cgtctgggtc
    11161 actgacatcg acacggttcc cgcctcgatc acctcgatgc ggcgcctgct gcgtgacgtc
    11221 ctcgggctgg gggaggaccc tgcctggctg acggaggcgg accgggcctg gagtgggccg
    11281 gtgcccgccc cgagcgcccg cgtcgtcgtg ccgatctggc gtgatccgtg gatggtcatc
    11341 gggcggccga ctttcacggc cgacctgttg gcgcgctccg gtttcgaggt cgtgacggcc
    11401 ggggtgaccg gccgctatcc ggggctcgaa ccggaggtca tcgacgatcc tgcgctggcg
    11461 gacctcgtgc tcctgccgga cgagccgtac gtgttcacgg ccgctgacgg gcccgaggtg
    11521 ttcgagcgac tgccgacccg actggtgtct ggccggctca tcacctggta cggcccgtcc
    11581 ctgatcgagg cgcggatttc actggaccga tcctgagcgt gtactgtctc cgtggcgccc
    11641 agcggtctac cgcaggtagc gcacgccccc ttagctcagt cggcagagcg tttccatggt
    11701 aaggaaaagg tcgtcggt
//
LOCUS       DAOSOC010000071        11715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5452_length_11715_cov_9.133276, whole genome shotgun sequence.
ACCESSION   DAOSOC010000071 DAOSOC010000000
VERSION     DAOSOC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5452_length_11715_cov_9.133276"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PL014_06965"
     CDS             <1..600
                     /locus_tag="PL014_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT56865.1"
                     /translation="EHLYEMDYLKEGIGLRAMAQRDPLVEYQREGFALWNTMNEAVKE
                     ESVGLLFHVDVQVDQPAEAAPAVAPVHVSEMFGGLAGSGATADADAAPSPVQDDVPDA
                     VAASSGGAPADGVQDTSGAPAAEAAPKVEVSGVGVGRPRTGALHYTAPSESGGVEERD
                     ERSPASASALTDDQLRTTPKNSPCPCGSGKKFKMCHGRR"
     gene            765..1592
                     /locus_tag="PL014_06970"
     CDS             765..1592
                     /locus_tag="PL014_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56866.1"
                     /translation="MADARTSPGSRVVPRVSPDAQRALDVTLGLAGMTVGAAVVAVRT
                     AHRVTAPVTRLLWRPPLLPRSLHPATILEEAARTGRAEQVALRRRFEVLLDAWAPVLV
                     EAVVERIDLTRLVREHVDLDALVASVDLDAAVARVDLDAAVARVDLDAIAATLDVDAV
                     VARADLDAAVARVDIEAILDRVDLDAIAATLDVDAVVARADLDRVIARIDIVRIVEEV
                     LDAIDLPAIIRDSTGSMASETVRGVRMTGVSADDALSRVVDRALFRRRHPERPGGPA"
     gene            1589..2143
                     /locus_tag="PL014_06975"
     CDS             1589..2143
                     /locus_tag="PL014_06975"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT56867.1"
                     /translation="MTGEGAAPELSSIPREARRFQGTPAGIVTRFVANSIDALVVAVM
                     LCTMYLGYSAIIFMLDPRTFEFPAVTFLASLTVALVVATLYLWLAWWLLGRSYGAHVM
                     GIRVAGRRGRKLGPLRALARAWFCVFFPIGLFWCVVSPHRRSVQDVVLFTSVVYDWRP
                     RTPAGPDPQPPAVGESPGMPGAAS"
     gene            2196..3836
                     /locus_tag="PL014_06980"
     CDS             2196..3836
                     /locus_tag="PL014_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT56868.1"
                     /translation="MGTSAGTTTATAASAGVLATLAASQFLMTLDSSVMNVSIATVAS
                     DIGTDVTGIQTAITLYTLVMASLMITGGKVGSILGRKKAFALGCVIYGVGSFTTSLAP
                     NLAVLLIGWSLLEGIGAALIMPAIVALVATNFDRPDRPRAYGLVAAAGAVAVAAGPLI
                     GGALTTYASWRWVFAGEVVIVAIILAMTGRMADGESDRDARLDLGGTALSALGLGLVV
                     FGILRAGAWGFVNPKPGAPVWIGLSPTIWLMLGGGLVLLVFAAWEKRRVEAGQSVLID
                     PTILRNPVLRGGLTAFLFQFLLQAGLFFCVPLYLSVALGLSAVETGIRILPLSITLLL
                     AAVGVPKIWPQASPQRVARFGFLGLLAGIVSLMGALELGAGPEIVTLPLLLAGLGIGA
                     LASQLGAVTVSSVPDEQSPEVGGLQNTFTNLGASVGTALAGAILVSALTTSFFTGIQD
                     NPAIPETLTSKAQVEFAGGIQFIPDSDLEAALSKAGITGDTAQAIMEENSQSRIAGLR
                     AALAALAVLAIAALFSTGSLPTRQGQSDADAKVPAGLS"
     gene            complement(3890..4444)
                     /locus_tag="PL014_06985"
     CDS             complement(3890..4444)
                     /locus_tag="PL014_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HOT56869.1"
                     /translation="MSAAPARAAAAPPARLRVLPEPLALDEAMRRSRLAQQERLADAG
                     YVQGTLAVSFDTAPDDRLFGPQRTARDDLPDPAWWAGRITHALIEVMTGLRPAPQVVR
                     WTSPEVYAVVARRSALAARRAADPGARTRRQRLTVVRVRVCEPVNGVAEAAVVLQHGS
                     RVRAVALRLEGQDGKWRVTALQIG"
     gene            complement(4441..5205)
                     /locus_tag="PL014_06990"
     CDS             complement(4441..5205)
                     /locus_tag="PL014_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT56870.1"
                     /translation="MQTSSNSIKPMVVGLRGTLGLGVALALAVMLWRLVERTVAAVGA
                     GRPAQADELLIGLTASLALACVLWLALGLVLGLLAQVPGSLGRVALAVAEVLTPRLLR
                     QGAGFVLGVGVVAGLAPGTAVAEPPSPSSSISQAWSTTAGPDAVSPSLPDPGFHSLPD
                     PSWVPAAPTVRPQADVGLLSRAPADTVDTRPDVVVQRGDTLWTIAARHLGPGASDAEI
                     AQAWPAWFDANRGVIGDDPDLVLPGQVLRAPEVTVQ"
     gene            5282..5917
                     /locus_tag="PL014_06995"
     CDS             5282..5917
                     /locus_tag="PL014_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56871.1"
                     /translation="MRWEGLFADLEGQLAAEERRELDDEVAERTRRERALVTLADRLA
                     GAVGSTVRLGLGAGLHVQGDIADLGDGWVLLRDPATGREHLVPTAAVVTVSALGARVE
                     TARAARRFGLGYALRALSRDRATVAISLSGVGASAGAGVSVGPGAGPGSGVGFGWSAA
                     GGSVLVGTIDAVGADHLDLAEHPEGVPRRRGNVTAMTTVPFQALVLVESRR"
     gene            complement(5925..6161)
                     /locus_tag="PL014_07000"
     CDS             complement(5925..6161)
                     /locus_tag="PL014_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT56872.1"
                     /translation="MARRFLPLTEVSEILDISAAQAYALVRSGDLPAIKVGGRGQWRV
                     ETVELEAYIQRMYAETRELVSTRSDVTEQDEHRP"
     gene            6264..6935
                     /locus_tag="PL014_07005"
     CDS             6264..6935
                     /locus_tag="PL014_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56873.1"
                     /translation="MTDLPTTTASRLRPPSWRDSRLIIGVLLVLLSTVLGSVVVARAD
                     DRVPVYAAKGEVAPGQQLRAADVVRVDVLLGDGAVGYLPADGPLPDDTWALRALHPGE
                     LIPQAAVGRSDAVTVQLVALLVDSTSASALTAGSVVDVYVNRPKEGTTIGLPTYAGPE
                     RVLEGVSVVRVAGEDDVLGGATASRAVQVMVPREAVRDLVADVDLGARITLVPVPGGA
                     AAAAP"
     gene            6932..8395
                     /locus_tag="PL014_07010"
     CDS             6932..8395
                     /locus_tag="PL014_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56874.1"
                     /translation="MTSVLAALTDRHEAEFVRALEAAPDLELVRRCADVAELLSAGAA
                     GAARVAVVSPDLRGLDRDALRHLAGHGVRVAGLVARGDDEGERRLRQLGVTLLLTPGE
                     PPDAVSLALSGLAGDLAPSGTGRAGGGGGAGGSDGNGGSDGGVAGSPWGVDEAGNPVP
                     DAGQRLVGAGHDPTAGHDDGFEGHVPTPITVVWGPTGAPGRTTVAVTLAAQLAAGGVR
                     TLLVDLDTWGASVAQVLGLIDEAPGVAAAARASEQGSLDAVGLARVAPEVVPGLRVLT
                     GLPKAERWPELRAAAVEDVLRLARQVVEHVVIDVGFALEDDEELSYDTVAPRRNATTL
                     TALEQADQLVVVGSADPVGLQRLVRGVQEVAVLPSPRPVIVVNKVRASVAGARPERSI
                     ADVLSRFAGMETVRFLPWAPDDCDAALLSGRSLLEVAPQGALTSALAGLAADLEPRMP
                     STARPRRRGRRRAPTSGLRAAVGSATASVLPGRRRAV"
     gene            8408..9838
                     /locus_tag="PL014_07015"
     CDS             8408..9838
                     /locus_tag="PL014_07015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOT56875.1"
                     /translation="MVDRLSSLDTSFLHLEDAATPMHVGSVMVFDAPEGGFDYESLVT
                     LISERIAHVPRYRQKIKSVPGGLANPVWVDDKHFDMSYHVRRSALPRPGSDEQLEELV
                     ARIQPRPLDRTRPLWEVYLVEGLAEDRFAIITKTHHSLVDGISAVDIGNVLVDGNPTA
                     SDGVLTTWRARAEPSGVELVVGAVAEAVRTPSQVVETVQHGIADVGKTLGKVTSVAGE
                     VISTLARVSARPAPESPLNASVGRARRYVMIGTDLDDYRKIRTRLGQGAFADEVTIND
                     VILATIAGAFRSWLLTRGEAVYGGTTIRAMVPVSVHEGPDAPTGAQMTACFVDLPVGE
                     PGPSMRLHQIAFSMRQQMEGGSRRAVSADTLAGLGGFAPPTMHALGARLGGVVSKRLY
                     NVVITNVPGPQTPLYAAGARMVSTYPVTPLARGQALSIGLTSYDGGVYYGLYADRDAM
                     PDADVLGRGVVDSLHELLEAPRPKTR"
     gene            9869..10300
                     /locus_tag="PL014_07020"
     CDS             9869..10300
                     /locus_tag="PL014_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56876.1"
                     /translation="MPLARIYLPLTSADLADLAEGRPVGPAPLAAHAVTPLLGRTGLV
                     VDEEELEHAAWLAATREASGLTQGGAVRRVIAAADVDVALVLHPTEPDVPSRVHVAAP
                     LERRRIVSFHIDEEAGDTEPTDLLWYDVTELADVRDLVDTP"
     gene            10418..>11715
                     /locus_tag="PL014_07025"
     CDS             10418..>11715
                     /locus_tag="PL014_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT56877.1"
                     /translation="MDAVTHVPAPINETVLDYAPGSPERAALEVALVELSGTKHDLPH
                     TIGGVRVEGKGPRLEVRQPHAHAKVLGVTRGATKTEAAAAVDAAVAAAPAWRDMAFDD
                     RAAILLKAADLLAGPWRAKINAATMLGQSKTAYQAEIDAACELIDFWRFNVHFARQII
                     AEQPPANSRGLWNRSDYRSLEGFVYAVTPFNFTAIAGNLPTAPALMGNTVVWKPSHSQ
                     QLAAQLTMELLEEAGMPPGVINMVNGHGREVSEVALAHPEFAGLHFTGSTAVFQGLWQ
                     QIGANLTGYRHYPRIVGETGGKDFVLAHPSADPDVLRTALIRGAFEFQGQKCSAASRA
                     YVPRSVWKVIKKDLVELTNGLSVGDVTDLSNFMGAVIDDRAFAKHKAAIDRAHATSHL
                     DVVAGGTYDDSVGYFVRPTIVESTDPSDEMFTTEYFGPILA"
BASE COUNT         1557 a         4216 c         4259 g         1683 t
ORIGIN      
        1 gagcacctct acgagatgga ctacctcaag gagggcatcg gcctgcgcgc gatggcccag
       61 cgcgacccgc tcgtggagta ccagcgcgag ggcttcgccc tgtggaacac catgaacgag
      121 gccgtcaagg aggagtccgt cgggctgctc ttccacgtcg acgtccaggt cgaccagccc
      181 gccgaggccg cccccgcggt ggcgcccgtg cacgtctccg agatgttcgg tgggctcgcg
      241 ggcagcggtg cgacggctga tgcggatgcc gcgccgagcc ccgtgcagga cgacgtgccg
      301 gatgccgtgg ccgcatcgtc gggcggtgcg ccggcggatg gtgtccagga cacgtcgggt
      361 gccccggccg ccgaggctgc cccgaaggtc gaggtctcag gagtcggcgt cggccgtccc
      421 cgcaccgggg ccctgcacta cacggcgccg agcgagagcg gtggggtcga ggagcgcgac
      481 gagaggtcac cggcatccgc gagtgccctg accgacgacc agctcaggac gacgccgaag
      541 aacagcccct gcccgtgtgg gtcggggaag aagttcaaga tgtgccacgg tcgtcgctga
      601 cggcccgcac cgaccactga cgccggggca gatctgacca gctggtcaga tctgccccgg
      661 cggcgtcgtc ggttcgcttc gaggtcaccc acgtggggtg attcacggcg ggccacggtc
      721 gagtaccttt ccctcagcag atctcctaca gacgggttca ggcggtggca gacgcgcgga
      781 cgtcaccggg ctcgcgcgtc gtgccccggg tatcacccga cgcccagcgc gccctcgacg
      841 tgaccctggg gctcgcgggg atgacggtcg gcgcggcagt cgtcgcggtg cgcaccgcac
      901 accgggtcac ggcacccgtg acccggttgc tgtggcggcc cccgttgctg ccgcggagcc
      961 tgcaccccgc gacgatcctg gaggaggcgg cccgtaccgg gcgggccgag caggtcgccc
     1021 tgcgccggcg gttcgaggtg ctcctcgatg cctgggcgcc ggtactggtc gaagccgtcg
     1081 tcgagcggat cgacctcacc cggctggtcc gggagcacgt ggacctcgac gccctggtcg
     1141 catcggtgga cctggatgcc gcagtggcgc gcgtggacct ggatgccgca gtggcgcgcg
     1201 tggacctgga cgcgatcgcg gcgaccctcg acgtggatgc cgtggtggcc cgtgccgacc
     1261 tggacgcggc ggtggccagg gtggacatcg aggcgatcct ggaccgggtc gacctggatg
     1321 cgatcgcggc gaccctcgac gtggacgccg tggtcgcccg tgccgacctg gaccgggtca
     1381 tcgcgcggat cgacatcgtg cgcatcgtcg aggaggtgct cgacgccatc gacctgccgg
     1441 ccatcatccg tgactccacc ggctcgatgg cctcggagac ggtgcgtggg gtgcggatga
     1501 cgggggtgtc cgccgatgac gccctcagtc gggtcgtcga ccgggcgctg ttccgcaggc
     1561 ggcatcctga gcggccggga gggcccgcat gaccggggag ggcgcggcac cggagctgtc
     1621 gtcgatcccg cgggaggcca ggcggttcca ggggaccccg gccgggatcg tcacccgctt
     1681 cgtggcgaac tcgatcgacg cgctcgtggt cgcggtgatg ctgtgcacca tgtacctcgg
     1741 ctacagcgcg atcatcttca tgctcgaccc gcgaaccttc gagttccccg cggtcacctt
     1801 cctggcgagc ctcaccgttg ccctcgtcgt tgcgacgctc tacctctggc tggcgtggtg
     1861 gctgctcggc aggagctacg gtgcgcacgt catgggcatc agagtcgcag gccggcgggg
     1921 ccgcaagctc gggccgctgc gggcgctggc ccgggcctgg ttctgcgtgt tcttcccgat
     1981 cggcctgttc tggtgcgtcg tcagccccca tcggcgctcg gtgcaggacg tcgtgctctt
     2041 cacctcggtc gtctatgact ggaggccccg cacgcccgcc ggtcccgacc cgcagccacc
     2101 cgcggtgggc gagtcgccag ggatgccggg cgcggcatcc tagggaggcc cggcggctgg
     2161 cgtcgggctg acgtcccctg accagagggc cacccatggg tacttccgca ggaaccacca
     2221 ccgccaccgc agcctccgct ggggtcctcg ccacgttggc ggcctcgcag ttcctcatga
     2281 cgctcgacag ctcggtcatg aacgtgtcga tcgcgacggt ggcctccgac atcggaaccg
     2341 acgtcaccgg catccagacg gcgatcacgc tctacaccct cgtcatggcg tcgctgatga
     2401 tcaccggcgg caaggtcggc agcatcctgg ggcgcaagaa ggcctttgcg ctcgggtgcg
     2461 tcatctacgg cgtcggctcg ttcaccacct cgctggctcc caacctcgcc gtgctgctca
     2521 tcggctggtc gctgctcgag gggatcggcg ccgcgctgat catgccggcg atcgtcgcac
     2581 tcgtcgcgac gaacttcgac cgacccgacc gaccccgggc ctacggcctg gtcgccgcgg
     2641 ccggtgcggt ggccgtcgcc gccggaccgc tcatcggtgg tgcgctcacg acctacgcct
     2701 cgtggcgctg ggtgttcgcg ggggaggtgg tcatcgtcgc catcatcctc gcgatgaccg
     2761 gccggatggc cgacggtgaa tccgatcgtg acgcccggct cgacctcggc gggacggccc
     2821 tgtccgccct cgggctcggc ctcgtcgtgt tcggcatcct gcgcgcgggg gcgtgggggt
     2881 tcgtcaaccc caagccgggt gccccggtct ggatcgggtt gtccccgacg atctggctca
     2941 tgctcggcgg cggcctcgtg ctgctcgtct tcgcggcctg ggagaagcgc cgggtcgagg
     3001 ccggccagtc ggtgctcatc gacccgacga tcctgcgcaa ccccgtgctg cgcggcgggc
     3061 tcaccgcgtt cctcttccag ttcctgctcc aggccgggct gttcttctgc gtccccctct
     3121 acctctccgt ggcgctcggg ctgagcgccg tcgagaccgg catccggatc ctgccactgt
     3181 cgatcacgct cctgctcgcc gcggtgggag tgccgaagat ctggccgcag gcgtccccgc
     3241 agcgggtcgc gcgcttcggg ttcctcgggt tgctggccgg catcgtgagc ctcatggggg
     3301 cactggaact gggcgccggg ccggagatcg tcacgctgcc cctgctgctg gccggtctgg
     3361 gcatcggcgc cctggcctcg cagctcggcg cggtcaccgt gtcgtcggtg cccgacgagc
     3421 agagccccga ggtggggggc ctgcagaaca ccttcaccaa cctcggtgcg tcggtgggca
     3481 cggccctcgc gggggccatc ctcgtgtcgg cgctgacgac gtcgttcttc accggcatcc
     3541 aggacaaccc cgccatcccg gaaacgctca cctcgaaggc gcaggtcgag ttcgccgggg
     3601 gcatccagtt catccccgac tccgacctgg aagcggcgct cagcaaggcc gggatcaccg
     3661 gcgacaccgc gcaggcgatc atggaggaga actcacagtc gcgcatcgcg gggcttcgtg
     3721 cggccctggc agccctcgcc gtcctggcga tcgccgccct gttcagcacc ggcagcctgc
     3781 ccacgcgaca ggggcagtcg gacgcagacg cgaaggtgcc cgcgggcttg tcgtagccgc
     3841 ggcgccacgg gcacgtcgtc gccgagcacc ttccgggtgc tgggcccact cagccgatct
     3901 ggagggcggt gacccgccac ttgccgtcct ggccctcgag gcgcagcgcc acggcacgca
     3961 ccctcgaccc gtgctggagg accacggcgg cctcggcgac cccgttgacg ggttcacaca
     4021 cgcgcaccct gacgacggtg aggcgttggc gtctcgtgcg agcgccgggg tcggctgccc
     4081 gcctggccgc gagggcgctg cgtcgagcga cgacggcgta gacctcgggg gaggtccacc
     4141 gaacgacctg aggggcgggc cgtagcccgg tcatcacctc gatcagggcg tgggtgatgc
     4201 gacctgccca ccaggccggg tcggggaggt cgtcgcgcgc cgtgcgctgt gggccgaaca
     4261 gcctgtcgtc aggtgccgtg tcgaaggaga ccgcgagagt cccctggacg tagccggcgt
     4321 cggcgaggcg ctcctgctgc gccagtcgtg atcggcgcat cgcctcgtcg agggcgagtg
     4381 gctcggggag cacgcgcagg cgggcgggag gggccgctgc ggctcgggcg ggtgcggcgc
     4441 tcactggacc gtcacctcgg gcgcgcgcag gacctggccg gggaggacga gatcggggtc
     4501 gtcgccgatg acgccacggt tcgcgtcgaa ccacgcaggc caggcctgcg cgatctccgc
     4561 gtccgacgca ccgggtccga ggtggcgcgc ggcgatggtc cacagggtgt ctccgcgctg
     4621 gaccacgaca tcggggcggg tgtcgaccgt gtcggccggt gcgcggctga ggagccccac
     4681 atcggcctgg ggccgaaccg tcggggcggc ggggacccac gaggggtccg gaagggagtg
     4741 gaagcccggg tcgggcaggg agggggacac ggcatccggc ccagcagtgg tgctccaggc
     4801 ctgggagatc gagctcgacg gtgatggtgg ttcggcgacc gcagtgcccg gggcgagccc
     4861 cgccacgacc ccgaccccga ggacgaaacc cgccccctgt cgcaggaggc ggggggtgag
     4921 gacctccgcg acggccagag ccacccggcc gagcgaaccc ggcacctggg ccagcaggcc
     4981 gagcaccagc cccagcgcga gccacagcac gcaggcgagc gcgagggacg cggtgaggcc
     5041 gatgagcagc tcatcagcct gtgccggccg tccggcgccg acggccgcga ccgtgcgttc
     5101 gacgaggcgc cacagcatca cggcgagggc cagggcgacg ccgagaccga gggtgccccg
     5161 cagcccgacg accatcggtt tgatgctgtt tgatgatgtt tgcatgcgga tgacggtaag
     5221 tggggacggc gcgccccgca accggagggt gctcggctgt ggacatccct acagtggcgc
     5281 catgaggtgg gagggactct ttgcagacct cgaggggcag ttggcggcgg aggaacggcg
     5341 cgaactcgac gacgaggtgg ccgaacgcac ccgccgtgag cgggcgctcg tcacgttggc
     5401 ggaccgcctg gcgggcgccg tggggtcgac ggtgcggctg gggctcggtg ccgggctgca
     5461 cgtgcagggt gacattgctg acctcggcga cgggtgggtg ctgctgcgtg acccggcgac
     5521 cggtcgtgag cacctcgttc ccaccgcggc cgtggtcacg gtgagcgccc tcggggcccg
     5581 cgtcgagacg gcccgggccg cgcgccggtt cgggctcggg tacgccctgc gcgcgctctc
     5641 gcgcgatcgg gcgacggtgg cgatcagcct gtccggagtg ggtgccagcg caggggcggg
     5701 cgtcagcgtc ggccccggag cagggccggg ttcaggtgtc ggtttcggct ggtccgcggc
     5761 cgggggatcg gtgctcgtcg gcacgatcga tgccgtgggg gctgatcacc tcgacctcgc
     5821 cgagcacccg gagggagtgc cgcgccgtcg gggcaacgtg accgccatga cgaccgtgcc
     5881 cttccaggcg ttggtgctgg tggagtcgcg gcgctgaccg gcgatcaggg tcggtgctcg
     5941 tcctgctcgg tgacgtcgga gcgggtcgag acgagctcgc gtgtctctgc gtacatgcgc
     6001 tggatgtagg cctcgagctc gacggtctcg acgcgccact gaccccggcc accgaccttg
     6061 atggcgggca ggtcaccgct gcgcacgagc gcgtacgcct gtgctgccga gatgtcgagg
     6121 atctcggaca cctcggtcag ggggaggaaa cggcgggcca cgtcgtcagt gtgccatcca
     6181 cagggtgggt gctccggcgg gttgtccaca gggcgctgcg ctcgacaggc gagatgcggt
     6241 caggtcaacc acaatcggtg cccatgaccg atctgccgac caccaccgcg tcccgactgc
     6301 ggcccccgtc gtggcgcgac tcacgcctga tcatcggcgt cctcctcgtg ctgctgtcga
     6361 cggtgctcgg ctcggtggtc gtcgcccggg ccgacgaccg ggtgcccgtc tacgcggcca
     6421 agggtgaggt cgcgccgggg cagcagctga gggccgccga cgtcgtgcgg gtggacgtcc
     6481 tgctcggcga cggcgcggtg ggttacctgc ccgccgacgg ccccctaccc gacgacacct
     6541 gggccctgcg cgccctgcac cccggcgaac tcatccccca ggccgccgtc ggcaggtcgg
     6601 atgccgtgac ggtgcagctc gtcgcgctcc tcgtcgactc cacgtccgcc tcggccctga
     6661 cagccggatc ggtcgtcgac gtgtacgtca accgccccaa ggagggcacg acgatcggcc
     6721 tccccacgta tgccgggccc gaacgggtgc tcgaaggcgt gtcggtggtg cgcgttgccg
     6781 gtgaggacga cgtcctcggg ggcgcgacgg catcccgtgc cgtccaggtg atggtgccgc
     6841 gagaagccgt gcgcgacctc gtcgccgatg tcgacctcgg tgcccggatc accctcgttc
     6901 cggtgcccgg cggcgccgcg gcggccgccc cgtgaccagc gtcctcgcag ccctcaccga
     6961 ccgccacgag gccgagttcg tccgggcgct cgaggccgcc cccgacctcg agctcgtgcg
     7021 gcggtgcgcc gacgtggccg agctgctgtc ggccggggcc gccggtgccg cccgcgtcgc
     7081 cgtcgtctca cccgacctgc gtggtctcga tcgtgatgcg ctgcgccacc tcgccgggca
     7141 cggcgtgcgc gtcgcaggtc tcgtggcacg cggcgacgac gagggggagc gcaggctgcg
     7201 ccagctcggc gtcacgttgc tcctcacacc gggggaaccg ccggatgccg tctccctcgc
     7261 cctgagcggg ttggccggtg acctggcccc ttccgggacg gggcgggccg gcggcggggg
     7321 cggagcaggt ggctccgacg gcaacggggg aagcgacggc ggcgtcgcgg ggagcccgtg
     7381 gggcgtggac gaggcaggaa accccgtgcc cgacgctggg cagcggctcg tcggcgccgg
     7441 ccacgacccc acggctggac acgacgacgg cttcgagggg cacgtgccga ctccgatcac
     7501 cgtggtctgg ggcccgacgg gtgccccggg gcgcaccacc gtggccgtga cgctggcggc
     7561 gcagctggcc gccgggggag tgcgcacgct gctcgtcgat ctcgacacct ggggtgccag
     7621 cgtggcccag gtgctcggcc tgatcgacga ggccccggga gtcgccgcag cggcgcgggc
     7681 ctccgagcag ggctcgctcg atgcggtcgg cctggcccgg gtcgcgcccg aggtggtgcc
     7741 cggcctgcgg gtgctcacgg ggctgcccaa ggccgagcgc tggcccgagc tgcgggcggc
     7801 cgcggtggag gacgtccttc gcctggcccg acaggtcgtc gagcacgtcg tcatcgacgt
     7861 cggcttcgcc ctcgaggacg acgaagagct gagctacgac acggtggccc cgcgtcgaaa
     7921 cgccacgacc ctgaccgccc tcgagcaggc cgaccagctc gtcgtcgtcg ggtccgccga
     7981 cccggtcggg ctgcaacggc tcgtgcgtgg tgtgcaggag gtcgcggtgc tgccctcgcc
     8041 acgacccgtc atcgtcgtga acaaggtccg cgcgtcggtg gccggggcga gacccgaacg
     8101 ctcgatcgcc gacgtcctca gccggtttgc cggcatggag accgtgcggt tcctgccgtg
     8161 ggcccccgac gactgtgacg cggccctgct ctcggggcgt tccctgctcg aggtcgcacc
     8221 ccaaggggcg ctgacctccg ccctggcggg actggcggcc gacctcgaac cacggatgcc
     8281 gtcgacagca cgaccccgcc gccgcgggcg gcgtcgggcc ccgacctcgg gcctgcgcgc
     8341 cgctgtcggc agtgccaccg cgagcgtcct gcccgggcgg cggcgggcgg tctgacacga
     8401 tagggccgtg gtagaccggc tcagctccct cgacacctcg ttcctccacc tcgaggacgc
     8461 ggccacgccg atgcacgtcg gctcggtgat ggtcttcgac gcgcccgagg gcggcttcga
     8521 ctacgagagc ctcgtgaccc tcatcagcga gcggatcgcg cacgtcccgc gctaccggca
     8581 gaagatcaag tccgtccccg gcgggctggc caacccggtc tgggtcgacg acaagcactt
     8641 cgacatgagc taccacgtgc ggcgcagcgc cctgccgcga ccgggctcgg acgaacagct
     8701 cgaagagctc gtcgcgcgca tccagccacg tcccctcgac cgcacccggc ccctgtggga
     8761 ggtctacctc gtcgagggtc tggccgagga ccggttcgcg atcatcacca agacccacca
     8821 cagcctcgtc gacggcatca gcgccgtgga catcggcaac gtcctcgtcg acggaaaccc
     8881 cacggcgagc gacggcgtcc tcaccacgtg gcgtgcccgc gccgagccga gcggcgtcga
     8941 actcgtcgtg ggagcggtcg ccgaggcggt tcgcaccccg agccaggtcg tcgagacggt
     9001 ccagcacggc atcgccgacg tcggcaagac cctcgggaag gtgacctcgg tcgccggtga
     9061 ggtcatctcg accctggcgc gggtctccgc gcggccggcg ccggagtcgc cgctcaacgc
     9121 ctcggtgggg cgggcgcggc gctacgtcat gatcggcacc gacctcgacg actaccgcaa
     9181 gatccgcacc cgattgggcc aaggtgcctt cgccgacgag gtcaccatca acgacgtcat
     9241 cctcgcgacg atcgccggtg ccttccggtc ctggctgctc acccgcggcg aggccgtcta
     9301 cggcgggacg accatccgcg ccatggtgcc ggtctcggtg cacgagggcc ccgacgcccc
     9361 gacgggcgcc cagatgacgg cgtgcttcgt cgacctgccg gtgggggagc ccgggccgtc
     9421 gatgcgcctg caccagatcg ccttctcgat gcgccagcag atggagggcg gctcgcggcg
     9481 cgccgtctcg gccgacaccc tcgccgggct cggtggcttc gcacctccga cgatgcacgc
     9541 cctcggcgcc cgcctcggtg gtgtggtttc caagcgcctc tacaacgtcg tcatcaccaa
     9601 cgtgcccggg ccgcagacgc cgctctacgc ggccggtgcc cgcatggtct ccacctaccc
     9661 ggtgacgccg ctcgcgcggg gtcaggccct gtcgatcggg ctcacctcgt acgacggtgg
     9721 tgtctactac ggcctctacg ccgaccgcga tgccatgccg gatgccgatg tgctcggccg
     9781 cggtgtcgtc gactcgctcc acgagctgct cgaggccccc cgccccaaga cccgctgacc
     9841 gaccaccgcg ccgcaccgag gagcccgtat gccgctcgcc cgcatctacc tcccgctcac
     9901 gtcggcggac ctcgccgacc tggccgaggg tcgccccgtg ggcccggccc cgctggccgc
     9961 ccacgccgtg accccgctgc tcggacgaac cggtctcgtc gtcgacgagg aggaactcga
    10021 gcacgccgcc tggctggcgg ccacgaggga ggcgagcggg ctgacgcagg gcggtgcggt
    10081 gcggcgggtc atcgccgcgg ccgacgtcga tgtcgccctc gtgctccacc cgaccgaacc
    10141 tgacgtgccg tcgcgggtcc acgtggcggc acccctcgag cgtcgccgga tcgtgtcgtt
    10201 ccacatcgac gaggaggcgg gcgacaccga gccgaccgac ctgctctggt acgacgtcac
    10261 cgaactggct gacgtgcgcg acctcgtcga caccccctga cccctgcgac gtcggggggg
    10321 agcgaggttg cacccgggcg tggtgggcga gagagtggcg gggtgatccc ggcgtgggga
    10381 ccccacgcgt caatcgaccc aaggagtgcc accacccatg gatgccgtga cccacgtccc
    10441 tgccccgatc aacgagaccg tcctcgacta cgccccgggc agtcccgagc gggcggccct
    10501 cgaggtcgcc ctggtcgagc tgtccggcac caagcacgac ctgccgcaca ccatcggcgg
    10561 ggtgcgggtt gagggcaagg ggcctcgcct cgaggtgcgt cagccgcacg cccacgccaa
    10621 ggtgctcggc gtgacccgcg gggccacgaa gaccgaggct gccgccgccg tcgacgccgc
    10681 cgtcgctgcg gctccggcct ggcgggacat ggccttcgac gaccgtgcgg cgatcctgct
    10741 caaggccgct gacctgctgg ccggcccgtg gcgcgcgaag atcaacgccg cgacgatgct
    10801 ggggcagagc aagacggcat accaggccga gatcgacgcg gcgtgcgagc tgatcgactt
    10861 ctggcgcttc aacgtccact tcgcccgaca gatcatcgcg gagcagccgc cggcgaactc
    10921 ccgagggctg tggaaccgca gcgactaccg ctcgctcgag ggtttcgtct acgcggtgac
    10981 gccgttcaac ttcaccgcga tcgccggcaa cctgccgacc gccccggcac tgatgggcaa
    11041 caccgtcgtg tggaagccct cgcactcgca gcagctcgcc gcccagctga cgatggagct
    11101 gctcgaagag gccgggatgc cgcccggcgt catcaacatg gtcaacgggc acgggcgcga
    11161 ggtctccgag gtcgccctcg cgcaccccga gttcgcgggg ctgcacttca ccgggtcgac
    11221 cgcggtgttc cagggcctgt ggcagcagat cggcgcgaac ctcaccgggt accggcacta
    11281 cccgcggatc gtcggcgaga ccggtggcaa ggacttcgtg ctggcccacc cgagcgccga
    11341 ccccgacgtg ctgcgcaccg ccctgatccg aggggccttc gagttccagg gccagaagtg
    11401 ctcggccgcg tcacgcgcct acgtgccccg ctcggtgtgg aaggtcatca agaaggacct
    11461 cgtcgagctc accaacggcc tgtcggtggg cgacgtcacc gacctgtcga acttcatggg
    11521 tgccgtcatc gacgaccggg ccttcgccaa gcacaaggcg gccatcgacc gggcccacgc
    11581 gacgagccac ctcgacgtcg tcgccggcgg cacctatgac gactccgtcg ggtacttcgt
    11641 gcggccgacg atcgtcgaga gcaccgaccc gagcgacgag atgttcacca ccgagtactt
    11701 cgggccgatc ctcgc
//
LOCUS       DAOSOC010000072        11680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5477_length_11680_cov_9.276903, whole genome shotgun sequence.
ACCESSION   DAOSOC010000072 DAOSOC010000000
VERSION     DAOSOC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5477_length_11680_cov_9.276903"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..631)
                     /gene="ybaK"
                     /locus_tag="PL014_07030"
     CDS             complement(146..631)
                     /gene="ybaK"
                     /locus_tag="PL014_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOT56878.1"
                     /translation="MSRRDPSGTPATVTLTAAGVPFSQHVYEHDPAARSYGQEAVDAL
                     GLPAEQMFKTLLVDTGAGLAVAVVPVAGSLDLKAMASALGVKRVGMADPAAAQRSSGY
                     VLGGISPVGQKRALPTVVDESAELFDVVYVSGGRRGFDIGLAPADLVRVTGAVLADIA
                     R"
     gene            complement(676..2121)
                     /locus_tag="PL014_07035"
     CDS             complement(676..2121)
                     /locus_tag="PL014_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOT56879.1"
                     /translation="MTQIIDQPYAYRRRELVEPDWTRLPGWRDVTPEQWADAQWQRAH
                     CIKNVAQLRTLMGDLLDERFYADLERDQAERATMSMLVPPQMMNTMVPATDGVMPGAG
                     AAFTEAFYADPIRLYMLPVFSDRRTDWPSHPFSTRDSLHEHDMWAVEGLTHRYPTKVL
                     AEMLPTCPQYCGHCTRMDLVGNSTPQFTKLKLAGKPVDRYAAMLDYLQRTPGVRDVVV
                     SGGDVANMPWKNLEGFLDKLLEVETIRDIRLATKALMGLPQHWLQPDVVEGVARISAK
                     ARSRGVSVAIHTHVNNAQSVTPLVAQAAQAMLDAGVRDVRNQGVLMRGVNNTTKDLLD
                     LCFALQDEAMITPYYFYMCDMIPFAEHWRLSLREAQDLQHGIMGYLPGFATPRIVCDV
                     PFVGKRWVHQVESYETERGMSFWRKNYRTSIEAGDEQALSREYVYYDPIYTLPQAGQD
                     WWRSTTDLEATHEAALVGASASRAASAADLL"
     gene            2208..3260
                     /locus_tag="PL014_07040"
     CDS             2208..3260
                     /locus_tag="PL014_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOT56880.1"
                     /translation="MRTTPSSPVGMHRVVDPAGAALPQAARVLDAGGDLWPDEVRIDV
                     ETLNLDAASFRQLSEKHAGDGDAVRAEVLDIVATRGKMQNPVTGSGGMLIGTVAEVGP
                     QSPLGLAVGDRVATLVSLSLTPLQLTDGLERWDGRSERVPASGTAVLFGRSIAARLPE
                     DMSPDLALMVMDVCGAPALVSRVVGEYVDAGVSPTVVVLGGAGKSGSLSLAAARSAGA
                     GHLIGVVPFEREADQLTASGLADRVVIADARSPLGLADAVAAAGGPADVTVVCVDVPG
                     CEQPAILATAQGGTIIFFSMATSFSAAALGAEGLAADVRMLVGNGYVPGHADLALDLV
                     RGNAAVRALFEGRLSE"
     gene            3641..5710
                     /gene="pta"
                     /locus_tag="PL014_07045"
     CDS             3641..5710
                     /gene="pta"
                     /locus_tag="PL014_07045"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOT56881.1"
                     /translation="MKGLYLAGTEARSGKSAVAVGLVNRLTRRHGRVGVFRPVVQAGG
                     QDELLRVLLQEAHTDLAPELAWGVTHDDLHADHDRALGVVVDRFHAMAQGHDVVLVVG
                     SDFSDVVAPTEFATNARIAADLGTPLVLVAPARDREQAELAASAAAAVAAARAVHAHV
                     GGVVINQVRSDESAAALDALTTRLGALPLWALTETPLLRAPTVRDLMAACDGSLVHGD
                     AELLDRESMGLVVAAMSMPHVIDRLIEGSTVIVPGDRDDVVLGVLLAHHSRTLPTLSG
                     LVLNGGFALSPQIDRLIAGLDMRLPIVSCQLGTMDTATALGRVEGRIAATSARKVRAA
                     ADLLDRTEGIDALDALLSDDAQGGERAVTPLMFEHDLVERAREAQAHIVLPEGAEERI
                     IIAADQVLARGIARLTLLGDESVIRDRARVLGADISAAEVVDPATSPWREEFAATYAA
                     LRAHKGVTHDQAFDRVVDPSYFGTMMVHAGRADGMVSGCITTTAHTIRPALEVVRTAP
                     GVSVVSSVFLMCLADRVLVYGDCAVNPDPDAAQLADIAISSARTAAAFGIEPRVAMLS
                     YSTGESGTGADVEKVRAATAIVRELAPDLLVEGPIQYDAAVDPHVAATKLKGSPVAGR
                     ATVLVFPDLNTGNNTYKAVQRSADAVAIGPVLQGLNRPVNDLSRGALVRDIVNTVAIT
                     AVQGQPQ"
     gene            5707..6924
                     /locus_tag="PL014_07050"
     CDS             5707..6924
                     /locus_tag="PL014_07050"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT56882.1"
                     /translation="MSTDPHVFVVNAGSSSLKYQVINAASGHTVAVGLVSEIGGASRM
                     RHDSIGPGGEVSRHESDAPCQTHTSAFAAARAALREHGGGVGDHVVAIGHRVVHGGRR
                     FTQPVVVDDVVLESLRVLSPLAPLHNPANVEGIVRAQEAFPGLPHVAVFDTGFHGTLP
                     PAAYTYAVPDSWRDEHHVRRYGFHGSSYAWVTRRTAALMGRPVDDLRMVVLHLGNGAS
                     ACAVDGGRSVDTSMGLSPVEGLVMGTRSGDVDPALGGYLERVAGLTASDYDRALNRES
                     GLLGLGGVSDFRTIVERRDAGDAAATLAFDVTVHRLRKYVGAYAAVLGRLDALVFTGG
                     IGEHSAPLRAAVVDGLEVLGLALDAEANAGGPPERRISGEESRAEVWVVPTDEEREIA
                     RATLEVLGLPHAL"
     gene            6921..8468
                     /locus_tag="PL014_07055"
     CDS             6921..8468
                     /locus_tag="PL014_07055"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT56883.1"
                     /translation="MSTTLYRGGTVHTPDQPGATAFVVGDDGRIAWIGDDQGATSHRD
                     GVDTVLDLDGGLVLPAFVDAHVHLSHTGMGLRGVDLGATTSVAQALRHIEEAARRHGG
                     RPIFAFGWQEQAWAEGRPVTSVELDRASYGGVVYASRVDGHSAVVSSALAMMSAARGL
                     PGWTEQGFVTRDAKNAARASFDAAGSRQQRRDDIEAALREAAAVGLAVVHECGGPLLT
                     SAEDFADVLDLGTRPDLTRTVGYWAEPVTDPEQARALTALHGAAGLAGDLNIDGSIGS
                     RTALLRQPYADDPGCSGTAYRTADGVRDHVAACAAAGVQSGFHVIGDAGMDTALDGIE
                     AAATLVGLDTVRATRPRLEHAEMVDPEGIARMARLGIVASVQPSFDAAWGGPHGMYAE
                     RLGTARVPGTNPFAQLHEAGVALALGSDSPVTPFDPWGAVWGAVLHHEPGARLDVRSA
                     IAAHTAGGWAAAREVGGVLRVGAPATLAVWDVPDAGHDGLPQVTDGAPRPTCRRTLRD
                     GLVLHEA"
     gene            8596..10194
                     /locus_tag="PL014_07060"
     CDS             8596..10194
                     /locus_tag="PL014_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOT56884.1"
                     /translation="MSSRPRQILDLDAATVRRARTLARRAGKPVVDLARSHTTVSVER
                     ATLRLAGLAGADHENVPWVNHLVDAVREGAGLEHGVTLSVWDALRRGEATDLLTLAQK
                     AASGGVRFEVPQGKAATAAGKAGRTAIARGIRTIDRQRAARDRLIARHGNPPRKPWIY
                     LIVATGDIYEDIPQAQAAAREGADIIAVIRSTGQSLLDFVPEGATREGYAGTYATQEN
                     FRLMRAALDETSKELGRYVRLTNYASGLCMPEIAALAGLERLDMMLNDSMYGILFRDI
                     NPIRTFVDQRFSRQVHARAGIIINTGEDNYLTTADAVDAAHTVTVSQLLNEYFAKEAG
                     LEDWQLGLGHAFEIDPTVKDSFRLELAHALLARQLFPEAPLKWMPPTKHMTGDIFRGY
                     LLDGFFNLAGAMTGQGILLVGMMTEAVVTPWISDRDLALQNVRYVMDAAGGLTEDFHP
                     PRDGLIQTRARQVLGEAVDLLDTIVTDTSTAGVPPLLAAIADGTFGSMKRPPTKGKGL
                     EGVVRKADGYLNPATDLLETGETR"
     gene            10191..11057
                     /locus_tag="PL014_07065"
     CDS             10191..11057
                     /locus_tag="PL014_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOT56885.1"
                     /translation="MTRTEAQEARSRSRRHGAERRMEDALPGEPSLAEPGPIVRPYGD
                     TTGDGMVQVSFTLPLPHDKRAEGAALQLAAKMGLEPALLVHAKAMGPHFTFFVVYGSV
                     THLVDVRDVVVHEREFPLLSARDTNAEIRKRLRRKLVVVGACIGTDAHTVGIDAILNI
                     KGFAGEKGLEYYREMTVHNLGAQVLVPELVNTAREVRADAVLVSQVVTQKDAHIHNTQ
                     AMSAAFREAFPEGQVPLLVVGGPRFEEGSAASLGVDRIFGRGTTPGEVASYLVHALVT
                     PRTEPDPRGKQR"
     gene            11054..11491
                     /locus_tag="PL014_07070"
     CDS             11054..11491
                     /locus_tag="PL014_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOT56886.1"
                     /translation="MSPEPTDRSANGSSPGSRAEVGLVVTHRRYVPYSHAHYAGNLVD
                     GAYSLAAFGDVATEMCIRTDGDEGLFASYSDVQFRAPVRAGDVIEISATLVRVGSRSR
                     EMDFEVRVVGRGAPERGESAADVLDPPVVATTARGVVVVPPAG"
BASE COUNT         1625 a         4357 c         4052 g         1646 t
ORIGIN      
        1 gacgcagcac ctcggaacgc acgcagcact tcagaccgga cgcagcactt cggatcgcgc
       61 gcgataattg ctgcgtgcca tccgaacttc tgcctcgggg gatcacttct gcgtgcgatc
      121 cgaacttctg cgttcggggc tgcgttcagc gggcgatgtc ggcgaggacg gcacccgtga
      181 cccgcaccag gtccgccggg gcgagaccga tgtcgaaccc gcgccgccca cccgagacgt
      241 agacgacgtc gaacagctcg gcactctcgt cgaccaccgt cggcagggcc cgcttctgcc
      301 ccacggggga gatgccaccg agcacgtacc ccgaggaccg ttgcgcggca gccggatccg
      361 ccatgcccac ccgcttgacc ccgagcgccg acgccatcgc cttgaggtcc agcgagcccg
      421 cgacgggcac caccgcgacc gcgagaccgg ccccggtgtc gacgagcaac gtcttgaaca
      481 tctgctcggc aggcaggccg agcgcgtcga cggcctcctg accgtaggac cgggccgccg
      541 ggtcgtgctc gtacacgtgc tgggagaacg ggacacccgc cgcggtgagg gtcaccgtgg
      601 cgggtgttcc ggaagggtct cgacgcgaca tacgtcgcag cgtagaacca cccccggagc
      661 cgggggaggg ctgccctaca gcaggtcggc ggcggatgcc gcgcggctgg cgctcgcacc
      721 cacgagggcg gcctcgtggg tcgcctcgag gtcggtggtc gagcgccacc agtcctgccc
      781 ggcctgcggc agggtgtaga tcgggtcgta gtacacgtac tcgcgcgaga gtgcctgctc
      841 gtcgccggcc tcgatggagg tgcggtagtt cttgcgccag aacgacatcc cgcgctcggt
      901 ctcgtagctc tcgacctggt gcacccagcg cttgccgacg aacggcacgt cgcacacgat
      961 gcgcggcgtc gcgaacccgg gcaggtagcc catgatcccg tgctggaggt cctgggcctc
     1021 gcgcagcgac agccgccagt gctcggcgaa cgggatcatg tcgcacatgt agaagtagta
     1081 cggggtgatc atcgcctcgt cctggagggc gaagcacagg tcgagcaggt ccttggtagt
     1141 gttgttcacc ccgcgcatga gcacgccctg gttgcgcacg tcgcgcaccc ccgcgtcgag
     1201 catcgcctgg gccgcctgcg ccacgagcgg cgtcacggac tgggcgttgt tgacgtgggt
     1261 gtggatcgcg acggacacgc cgcgcgagcg cgccttggcg ctgatccggg ccacaccctc
     1321 gacgacgtcc ggctgcagcc agtgctgcgg caggcccatg agcgccttgg tcgcgaggcg
     1381 gatgtcgcgg atggtctcga cctcgagcag cttgtcgagg aacccttcga ggttcttcca
     1441 cggcatgttc gccacgtcac caccggagac cacgacgtcg cgcacgcccg gggtgcgctg
     1501 gaggtagtcg agcatcgccg cataccggtc aaccggcttg cccgcgagct tgagcttggt
     1561 gaactgcggc gtcgagttgc cgacgaggtc catgcgggtg cagtggccgc agtactgggg
     1621 gcaggtcggc agcatctcgg ccagcacctt ggtcgggtac cggtgggtca gcccctcgac
     1681 ggcccacatg tcgtgctcgt gcaggctgtc gcgcgtggag aacgggtggc tcggccagtc
     1741 ggtgcgccgg tcgctgaaga ccggcagcat gtacagccgg atggggtcgg cgtagaacgc
     1801 ctcggtgaag gcggcaccgg caccgggcat gacgccgtcg gtcgcgggca ccatcgtgtt
     1861 catcatctgc ggcggcacga gcatcgacat cgtggcgcgc tcggcctggt cgcgctcgag
     1921 gtcggcgtag aaccgctcgt cgagcaggtc gcccatgagg gtgcgcagct gcgcgacgtt
     1981 cttgatgcag tgggcccgct gccactgcgc gtcagcccac tgctcggggg tgacgtcacg
     2041 ccaccccggc aggcgggtcc agtcgggctc gaccagttcg cgacgacggt aggcgtaggg
     2101 ctggtcgatg atctgcgtca tgcgtggcag gatacgcaat ggatgccgtc aaagtcacgt
     2161 ttgaccgtaa atgctgcggc tgcgtgttgg ccaatgagga gattcttgtg cgcaccaccc
     2221 cttcgtcccc cgtgggcatg caccgggtcg tcgaccccgc cggcgctgcc cttccgcagg
     2281 cggcccgtgt cctggacgcc ggcggcgacc tgtggcccga cgaggtccgc atcgacgtcg
     2341 agacgctcaa cctcgacgcg gcatcctttc gccagctgag cgagaagcac gcgggcgacg
     2401 gggacgccgt gcgcgcggag gtcctcgaca tcgtcgccac ccgcggcaag atgcagaacc
     2461 ccgtgacggg ctcgggcggc atgctcatcg gcaccgtcgc cgaggtcgga ccgcagtcgc
     2521 cactcgggct ggccgtgggc gaccgggtcg ccacgctcgt ctccctctcc ctgacgccgc
     2581 tgcagctcac cgacgggctc gagcgctggg acggtcgcag tgagcgtgtt ccggcatccg
     2641 ggaccgcggt gctgttcggt cggtcgatcg ccgcccgcct gcccgaggac atgtcacccg
     2701 acctcgccct catggtgatg gacgtgtgcg gtgcccccgc cctggtcagc agggtcgtgg
     2761 gggagtacgt cgacgcaggc gtgagcccca ccgtcgtcgt gctcggtggc gccggcaagt
     2821 cggggtcgct gtccctggcc gccgcgcgat ccgcgggggc cgggcacctc atcggcgtcg
     2881 tgccgttcga gcgggaggcc gaccagctga ccgcgagcgg cctggccgac agggtcgtca
     2941 tcgccgacgc ccgctcaccg ctcggcctcg cggatgccgt tgctgccgcc ggcggccccg
     3001 ccgacgtcac ggtggtctgc gtcgacgtgc cggggtgcga gcagccggcg atcctcgcga
     3061 ccgcccaggg cggcacgatc atcttcttct ccatggcgac gagcttctcc gcggcagcgc
     3121 tgggggccga gggcctggcc gccgacgtcc ggatgctcgt cggtaacggc tacgtccccg
     3181 ggcacgccga cctggcgctg gacctcgtgc ggggcaacgc cgcggtccgg gcgctgttcg
     3241 agggccgcct gagcgagtag ttgctgctca cccgcggcgg gtgacgtccg ggcgcggccc
     3301 tgtccgtagc gtcaaaggca acgacacccg gttcccgcag aggaatcccg catgcacctc
     3361 accaccgccg ctcgcgcggc atccgccgtc accaccctca cgctggccct gctcatcgcc
     3421 ggctgctcct ccgattccga caccacggcg ccggccgcga gcgcagcggc cagtgtcgct
     3481 gcgagtgtca aggcgagtgc gagcggtgag gcctgcagtg ccttcgccga gctgaagaca
     3541 tccgtccagg acagggcttg cccaaaccgg gttccgcctt gttggcttgg cgcatgaacc
     3601 cgctccgctg ctcggtctcg acccacacca ccccggtcct gtgaaggggc tctacctcgc
     3661 cggcaccgag gcccgctcgg gcaagtcggc ggtcgcggtc gggctcgtca accggctcac
     3721 ccggcggcac gggcgggtgg gcgtcttccg cccggtcgtc caggccggag gtcaggacga
     3781 gttgttgcgg gtgctgctcc aggaggccca caccgacctc gcacccgagc ttgcgtgggg
     3841 cgtgacgcac gacgacctgc acgccgatca cgatcgggcc ctcggggtcg tggtcgaccg
     3901 cttccacgcc atggcccagg ggcatgacgt cgtcctcgtc gtggggtccg acttctccga
     3961 cgtcgtcgcg ccgaccgagt tcgccacgaa cgcgcggatc gccgcggacc tcggcacccc
     4021 tttggtgctc gtcgctcccg cccgagaccg cgagcaggcc gagctcgcgg ccagcgcagc
     4081 ggccgcggtc gccgcggccc gcgctgtgca cgcccacgtc ggtggggtgg tcatcaacca
     4141 ggttcggtcc gacgagagtg ccgccgccct cgacgccctc accacccgcc tcggtgccct
     4201 cccgctgtgg gccctcaccg agaccccgct cctgcgggcg ccgacggtcc gcgacctcat
     4261 ggcggcctgt gacggcagcc tcgtgcacgg cgacgccgag ctgctcgacc gcgagagcat
     4321 gggtctggtc gtcgccgcga tgtcgatgcc gcacgtcatc gaccggctca tcgagggttc
     4381 caccgtcatc gtccccgggg atcgggacga cgtcgtgctc ggggtgctgc tcgcgcacca
     4441 ctcgcgcacg ctgccgacgt tgtccggcct cgtgctcaac ggcggcttcg ccctctcgcc
     4501 gcagatcgac cggctcatcg ccgggctcga catgcgcctg cccatcgtct cctgccagct
     4561 cggcaccatg gacaccgcga ccgcgctcgg gcgcgtcgag ggacgaatcg ccgcgacctc
     4621 ggcccgcaag gtccgtgcgg cggccgatct gctcgaccgc accgagggca tcgacgccct
     4681 cgacgccctg ctgagcgatg acgcacaggg cggtgagcga gcggtcaccc cgctgatgtt
     4741 cgagcacgac ctcgtcgagc gcgcccggga ggcgcaggcc cacatcgtcc tgcccgaggg
     4801 cgccgaggag cgcatcatca tcgcggccga ccaggtgctc gctcgcggga tcgcgcgcct
     4861 gacactgctc ggggacgaga gcgtcatccg cgaccgggcc cgggtgctcg gtgccgacat
     4921 ctccgccgcg gaggtcgtcg accccgcgac gagtccgtgg cgtgaggagt tcgcagccac
     4981 ctacgcggcg ctgcgcgcgc acaagggcgt cacccacgac caggccttcg accgggtcgt
     5041 cgacccgtcg tacttcggga cgatgatggt gcacgcaggc cgggccgacg ggatggtctc
     5101 cgggtgcatc acgaccacgg cgcacacgat ccgacctgcg ctcgaggtcg tccgtacggc
     5161 ccccggcgtc tcggtcgtct ccagcgtgtt cctcatgtgc ctggccgacc gggtgctcgt
     5221 ctacggcgac tgcgccgtga accccgatcc ggatgccgcc cagctcgccg acatcgcgat
     5281 ctcgtcggcg cgcacggcgg ccgcgttcgg catcgagccc cgggtcgcga tgctgtcgta
     5341 ctccaccggc gagtccggga ccggcgccga cgtggagaag gtgcgggccg ccacggccat
     5401 cgtgcgcgag ctcgcgcccg acctcctcgt cgaggggccg atccaatacg acgccgccgt
     5461 cgacccgcac gtggccgcca ccaagctcaa gggcagcccg gtcgcggggc gggccacggt
     5521 gctcgtcttc cccgacctca acaccgggaa caacacctac aaggcggtgc agcgcagcgc
     5581 cgacgcggtc gcgatcggcc ccgtcctgca gggtctgaac cgcccggtca acgacctctc
     5641 ccgaggagcg ctcgtgcgcg acatcgtcaa cacggtggcc atcaccgccg tccaggggca
     5701 gccccagtga gcaccgatcc gcacgtcttc gtcgtcaacg ccggctcctc gtcgctgaag
     5761 taccaggtga tcaacgccgc cagcggtcac acggtcgccg tcgggctcgt ctccgagatc
     5821 ggcggcgcga gccgcatgcg gcacgacagc atcggccccg gtggcgaggt ctcccgccac
     5881 gagtcggacg cgccgtgcca gacccacacc agcgccttcg ccgcggcccg cgccgcgctg
     5941 cgcgagcacg ggggcggcgt gggcgaccac gtcgtcgcga tcggccaccg ggtggtccac
     6001 gggggacgac gcttcaccca gcccgtcgtc gtcgacgacg tcgtcctgga gtcactgcgg
     6061 gtgctctccc cgctggcacc cctgcacaac ccggccaacg tcgagggcat cgtgcgggcc
     6121 caggaggcct ttcccgggct gccccacgtc gcggtcttcg acaccggctt ccacggaacc
     6181 ctgccgccgg ccgcgtacac ctacgccgtc ccggactcgt ggcgcgacga gcaccacgtg
     6241 cgccgatacg gcttccacgg cagctcgtac gcctgggtga cccggcgcac cgccgccctg
     6301 atgggccgac ccgtcgacga cctgcggatg gtcgtcctgc acctgggcaa cggggccagt
     6361 gcgtgcgccg tcgacggcgg tcgcagcgtc gacacgtcga tgggtctgtc cccggtcgag
     6421 ggcctggtca tgggcacccg ttccggcgac gtcgacccgg cgctgggggg ctacctcgag
     6481 cgcgtggcgg gactcacggc atccgactac gaccgcgccc tgaaccgcga gagcgggctg
     6541 ctcgggctcg gcggggtctc ggactttcgc acgatcgtcg agcgccgcga cgccggggat
     6601 gccgcggcga cgctcgcgtt cgacgtcacc gtgcaccggt tgcgcaagta cgtgggggcc
     6661 tatgccgccg tgctcggtcg cctcgacgcc ctcgtgttca ccggaggcat cggcgagcac
     6721 agcgccccgc ttcgggccgc cgtcgtcgac ggcctggagg tgctcgggct ggccctggac
     6781 gccgaggcga acgccggcgg gccacccgag cggcggatca gcggcgagga gagccgggcc
     6841 gaggtctggg tggtcccgac cgacgaggaa cgcgagatcg cgcgcgccac cctcgaggtc
     6901 ctcggccttc cccacgcgct gtgagcacca cgctctaccg cggcggcacc gtccacaccc
     6961 ccgaccagcc cggggccacc gcgttcgtcg tcggcgatga cgggcggatc gcgtggatcg
     7021 gtgacgacca gggcgccacg agccaccgcg acggggtcga cacggtgctc gacctcgacg
     7081 gcgggctcgt gctgccggcc ttcgtcgacg cccacgtcca cctgtcccac acgggcatgg
     7141 ggctgcgcgg tgtcgacctc ggcgccacca cctccgtcgc ccaggcgctg cggcacatcg
     7201 aggaggccgc ccgccgccac ggcggtcgcc cgatcttcgc gttcgggtgg caggaacagg
     7261 catgggccga gggccgcccg gtgacgtccg tcgaactcga ccgggcgagc tacgggggag
     7321 tggtctacgc ctcgcgggtc gacggccact cggccgtggt ctccagtgcg ctcgccatga
     7381 tgtccgcggc gagagggttg ccgggatgga ccgagcaggg cttcgtcacc cgggacgcca
     7441 agaacgcggc gcgggcgtcc ttcgacgcag ccggcagccg gcagcagcgc cgcgacgaca
     7501 tcgaggcagc tctgcgcgag gccgcagccg tcggcctcgc ggtggtccac gagtgcggcg
     7561 gcccgctgct cacgagcgcc gaggacttcg ccgacgtgct cgacctcggg acccgacccg
     7621 acctgacgcg caccgtcggg tactgggcag agccggtcac cgacccggag caggcccgcg
     7681 ccctcacggc actgcacggg gccgcggggc tggccggtga cctcaacatc gacggttcga
     7741 tcggctcacg aacggccctg ctgcgccagc cctacgccga cgatcccggg tgctcgggca
     7801 ccgcgtaccg cacggccgac ggagtgcgag accacgtcgc ggcctgtgcc gccgcgggtg
     7861 tgcagagcgg cttccacgtc atcggtgacg cggggatgga caccgcgctc gacggcatcg
     7921 aggccgccgc gaccctggtc ggcctcgaca ccgtgcgggc caccaggccg cgactggagc
     7981 acgccgagat ggtcgacccc gagggcatcg cccggatggc gcgcctgggc atcgtcgcga
     8041 gcgtgcaacc gtccttcgac gccgcgtggg gaggaccgca cggcatgtac gcggaacgtc
     8101 tcgggactgc tcgcgtgccg ggcaccaacc cgttcgccca gctgcacgag gccggggtgg
     8161 cgctggccct ggggtccgac tccccggtga caccgttcga cccgtggggc gccgtctggg
     8221 gcgcggtgct gcaccacgag ccgggtgccc gcctcgacgt ccggtccgcc atcgctgcgc
     8281 acaccgcagg cggctgggct gcggcgcgcg aggtcggcgg tgtcctgcgt gtcggtgccc
     8341 ccgcgaccct ggccgtctgg gacgtgccgg atgccggcca cgacgggttg ccgcaggtca
     8401 ccgacggtgc cccgcgaccg acctgccggc gcacgctgcg cgacgggctg gtgctgcacg
     8461 aggcctgaac cttcgagcgt gagggcctcc acgtcgacgg acaccgtcgc ggggccaatc
     8521 gtcgacgcca acagatactg tcgtccacac ctgtgagtgc cgcaatactt gcgccatcct
     8581 tgctagattg accctgtgtc ttcacgccca cgccagatcc tcgacctcga cgccgccacc
     8641 gtccgtcgcg cgcgcaccct cgcgcgccgc gccgggaagc ccgtcgtcga cctcgcccgc
     8701 agccacacca ccgtctcggt cgaacgggcg acgctgcgcc tggccgggct cgccggagcc
     8761 gaccacgaga acgtgccgtg ggtcaaccac ctcgtcgacg ccgtccgtga gggcgccggc
     8821 ctcgagcacg gggtcaccct ctccgtctgg gacgccctgc gtcgcggcga ggccaccgac
     8881 ctgctgaccc tggcccagaa ggccgccagc gggggagtgc gcttcgaggt cccgcagggc
     8941 aaggcggcca cggcggccgg caaggccggt cgcacggcga tcgcccgcgg catccgcacc
     9001 atcgaccgcc agcgagcggc gcgtgaccgg ctcatcgccc gccacggcaa cccgccgcgc
     9061 aagccctgga tctacctcat cgtcgccacc ggtgacatct acgaggacat cccgcaggcg
     9121 caggccgccg cgcgcgaggg cgccgacatc atcgccgtga tccgctcgac gggtcagagc
     9181 ctgctcgact tcgtgcccga gggcgcgacc cgtgaggggt acgcgggcac ctatgcgacg
     9241 caggagaact tccgcctcat gcgcgcggcg ctcgacgaga cgagcaagga gctgggccgc
     9301 tacgtccgcc tgaccaacta cgccagcggt ctgtgcatgc ccgagatcgc cgcgctcgcc
     9361 gggctcgagc ggctcgacat gatgctcaac gactcgatgt acggcatcct ctttcgcgac
     9421 atcaacccga tccgcacgtt cgtcgaccag cgcttcagcc gccaggtgca cgcccgcgcc
     9481 ggcatcatca tcaacaccgg tgaggacaac tacctcacga ccgcggatgc cgtcgacgcc
     9541 gcccacacgg tcaccgtcag ccagctgctc aacgagtact tcgccaagga ggcgggactg
     9601 gaggactggc agctgggcct cggccacgcg ttcgagatcg accccacggt caaggacagc
     9661 ttccgcctcg agctcgcgca cgccctgttg gcccgccagc tcttccccga ggcaccgctg
     9721 aagtggatgc cgccgaccaa gcacatgacc ggcgacatct tccgcgggta cctcctcgac
     9781 ggcttcttca acctcgccgg ggcgatgacc gggcagggga tcctcctcgt cggcatgatg
     9841 accgaggccg tggtcacccc gtggatctcc gaccgcgacc tcgccctgca gaacgtccgg
     9901 tacgtcatgg atgccgcggg cgggctcacc gaggacttcc acccaccgcg cgacggcctc
     9961 atccagaccc gggcccggca ggtgctcggc gaggcggtcg acctgctcga caccatcgtc
    10021 accgacacca gcaccgcagg ggtccccccg ctcctggcgg cgatcgccga cggcaccttc
    10081 gggtcgatga agcggccccc gaccaagggc aagggtctgg agggcgtcgt ccgcaaggcc
    10141 gacggctacc tcaacccggc caccgacctt ctcgagacgg gggagacccg atgacccgca
    10201 ccgaggccca ggaggcccgc agccgcagcc gccgccacgg tgccgagcgc cgcatggagg
    10261 acgcccttcc cggcgagccg agcctggcgg agcccggccc gatcgtgcgc ccctacggcg
    10321 acacgaccgg cgacggcatg gtgcaggtgt ccttcaccct gcccctgccg cacgacaagc
    10381 gtgccgaggg tgccgcgctc cagctcgcgg ccaagatggg gctcgagccg gcgctgctcg
    10441 tgcacgccaa ggcgatgggc ccgcacttca cgttcttcgt cgtctacggg tcggtcaccc
    10501 acctcgtcga cgtgcgcgac gtcgtcgtcc acgagcggga gttcccgctg ctgtccgccc
    10561 gcgacaccaa cgccgagatc cgcaagcggc tgcgccgcaa gctcgtcgtc gtcggtgcgt
    10621 gcatcggcac cgacgcccac acggtgggga tcgacgcgat cctcaacatc aaggggttcg
    10681 cgggggagaa ggggctggag tactaccgcg agatgacggt gcacaacctc ggcgcccagg
    10741 tgctcgtgcc cgaactggtc aacacggcgc gcgaggtccg agcggatgcc gtgctggtct
    10801 cgcaggtcgt cacccagaag gacgcccaca tccacaacac ccaggcgatg tccgccgcgt
    10861 tccgcgaggc gttcccggag gggcaggtgc cgctgctcgt cgtcggcggc ccgcgcttcg
    10921 aggagggctc cgccgccagc ctcggcgtcg accgcatctt cggccggggc accaccccgg
    10981 gtgaggtcgc ctcctacctc gtgcacgccc tcgtcacccc ccgcacggaa cccgacccga
    11041 gagggaagca gcgatgagcc ccgaacccac ggacaggagc gccaacggca gttcccccgg
    11101 cagccgcgcc gaggtcggcc tcgtcgtcac ccatcgccgc tacgtgccct actcccacgc
    11161 ccactacgcg ggcaacctcg tcgacggggc ctactcgctg gcggccttcg gcgacgtggc
    11221 cacggagatg tgcatccgca ccgacggcga cgagggcctg ttcgcgtcct actccgacgt
    11281 gcagttccgc gcccccgtgc gcgccggtga cgtcatcgag atctccgcga ccctcgtgcg
    11341 ggtcgggagc cggtcgcgcg agatggactt cgaggtgcgt gtcgtcggtc gcggcgcccc
    11401 cgagcggggg gagtcggcgg ccgacgtcct ggacccgccg gtggtcgcga cgaccgcccg
    11461 cggtgtcgtg gtggtccccc cggcgggctg agtccagccg ggtcagcagc gtgaattacc
    11521 gtgggcgcgt gcccaccccc cttcggtatg ccgcgcgccc agcggccgcc ctcgccgcag
    11581 gcctgtgcct gtggctcgcc ttccctgacg ccgacctgtg gttcctcgcc ccggtcgggg
    11641 tggccctgct cggcgctgcg accctgacgg ccgggggacg
//
LOCUS       DAOSOC010000073        11610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5529_length_11610_cov_8.124016, whole genome shotgun sequence.
ACCESSION   DAOSOC010000073 DAOSOC010000000
VERSION     DAOSOC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5529_length_11610_cov_8.124016"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..325)
                     /locus_tag="PL014_07075"
     CDS             complement(35..325)
                     /locus_tag="PL014_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HOT56887.1"
                     /translation="MSTTASTRTVGVDIQESEANRSVVEAIEATNDGCTVQHMPGLVR
                     ITAPGRLVVTRESVEERLGREWETHEFQLAIVSYFGHIKEWDDDEIVIAWDH"
     gene            complement(344..1348)
                     /locus_tag="PL014_07080"
     CDS             complement(344..1348)
                     /locus_tag="PL014_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56888.1"
                     /translation="MQYELRTQVIEPPRKTFTHLVERYGDRPASRYEEGSIGIQPKEN
                     FHYRPLWDPAHDIYDEAFSAFRLTDPYSFTDPRQYYYAPYVTSRAAMADAVASTLDYL
                     DKRDLLSRMPDAWSALMGELLVPLRHYESGAQMVSCDIARFGFGTTITQCAAYAAFDR
                     IGNAQAVSRIGIALDGGTAALLGEAKTRWVEDASLQGLRRYMEELFLEKDWGKALIRL
                     DVLDRLIYGLFFGHLDDAAIDAGAGSYSLLAQHQASWFTDQRKWVDALYKAWVADPET
                     GSANKALLAATTTEVLAAAQAAIAPLAARADELVGAGAAAHVEATATTVRDAVAALTA
                     "
     gene            complement(1466..1906)
                     /locus_tag="PL014_07085"
     CDS             complement(1466..1906)
                     /locus_tag="PL014_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56889.1"
                     /translation="MTPFMLRVAEIVGTPQDQLPELDELAPMPETRISRRIATGTGAD
                     RQVAIRSLAEQLVSEANAILGQADDRLQLVDETLPHELAFSVTHAGRAARVSTTFEDG
                     TAYGRLVGDGFESELPHELDGPDALPDLIVRLLVESGVSHHHVP"
     gene            complement(1903..>2243)
                     /locus_tag="PL014_07090"
     CDS             complement(1903..>2243)
                     /locus_tag="PL014_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOT56890.1"
                     /translation="DSTGGLLAFARMDGAFALSGGIAQDKAWTVAGFNGIPTDEFYEA
                     ISNEDAVREGIAQRGRVAAFGGGVPVLVDGALVGAVGASGGSAVQDKQVATAGADAVA
                     AALTHGEDTP"
     assembly_gap    2244..2343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2416..3330)
                     /locus_tag="PL014_07095"
     CDS             complement(2416..3330)
                     /locus_tag="PL014_07095"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HOT56891.1"
                     /translation="MAVMRLGYAHVRVTDLAEAKDHYANTMGLYETLSEGNKVYYKAW
                     DEWDHHSVVLEEGGVGLVKYGWKVQYSSDIDEIEKKAQTFGVAVERMSKGENPEIGDG
                     IRFTTPSDHVFEVYHEATLIGTEVGTHNPDSFPRHLVGVGVPALDHSLITAEDPGMMD
                     RFLTDVFGFYATERVQTSLDDDADIIATWMTNSTQIHQLAVLAGPQGKLHHFAFRLGD
                     WTEVGRAADLFSMDDVPIDVGPTRHGITRGQTVYFFDPSGNRNEVFAGGYLAYPDRPM
                     NVWTPDALGKGVFYHARELNDAFTSVLT"
     gene            complement(3330..3719)
                     /locus_tag="PL014_07100"
     CDS             complement(3330..3719)
                     /locus_tag="PL014_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT56892.1"
                     /translation="MPTVTIHPTGEVIYLEPGETVLSGLYKAGFAYTVGCRRGGCAIC
                     KVDCRSGQFRYDHPVADTVLTEDEHLDGTCLTCRAVPDTDITIELRGENVRLVNTFLH
                     MINDKARQRAEAHAAAGLMAGTTPKEQ"
     gene            complement(3820..4563)
                     /locus_tag="PL014_07105"
     CDS             complement(3820..4563)
                     /locus_tag="PL014_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT56893.1"
                     /translation="MSDALVHRADDDLIAVITLDSAANRNALSRRLIAELTDHLAQAG
                     ADESLHGILLRSSHPVFCAGADLKEAATVDMVESARGIIALQRAIVAAPVPVVTRLDG
                     PVRAGGLGLVASSDIVVCADSVTVALTEVRLGLAAAVISIPLRARLSPRAASDWFLTG
                     RSVSATEARDQGLVTHVASADEMDAVVARVLDHLRAGARQGLVEAKRLLTTDLVAQFD
                     SHGDEMARLSGLLFHSEVAQQRMAAALRR"
     gene            complement(4636..5082)
                     /locus_tag="PL014_07110"
     CDS             complement(4636..5082)
                     /locus_tag="PL014_07110"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOT56894.1"
                     /translation="MADSPGCRGPASDQPTTPDETPASTSRPPVPDAVAHHLASATDA
                     GREFYGILRDIVQRHAPEATEEISYSMPVFLVDGKRLLHAAPWKAHLAIYPLPEERDL
                     DPQTTAALATHSSGASTLKLLYKKPFPTELVDAVVRAHLARLADGR"
     gene            complement(5223..5813)
                     /locus_tag="PL014_07115"
     CDS             complement(5223..5813)
                     /locus_tag="PL014_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOT56895.1"
                     /translation="MKPHSPGERSSSEESASPAESTPISDEPEHLTIGDAGEAAAAAL
                     ARARAAAKAKGLRPGLRPRRRVKDVPVGERTSTGRDPKLLGEQLDQFVVERGWAVDVA
                     VGAVIGRWPQIVGPEIALHCAPVVFEDGVLTVRADSTAWATQLRLLSSSLLARLAEDV
                     GEGTVSELRVLGPGAPSWSRGQRRANDGRGPRDTYG"
     gene            complement(5833..7038)
                     /gene="recF"
                     /locus_tag="PL014_07120"
     CDS             complement(5833..7038)
                     /gene="recF"
                     /locus_tag="PL014_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOT56896.1"
                     /translation="MHVRHLSVVDFRSYTSAELPLEPGVTTLVGLNGQGKTNLVEALG
                     YLASLSSHRVATDAPLVRFGASQAVIRGAVVRDGRESMVELEINPGRANRARLNRSPL
                     TRPRDVLGTLRTVLFAPEDLALVKGDPGERRRFLDDLLVARQPRWAGVRSDYDKILRQ
                     RGALLKSAAPVLRRGGRRGARTASELGSEAAAQRDSALHTLDVWDSQLAGAGSHLLYA
                     RLRLLRDLGPFLAAAYDEVSAGPANATLTYRSSLRAETAERIAAGEVPEVDELESEML
                     ATLAEVRAQEIERGVCLVGPQRDDVILGLGELPAKGYASHGESWSFALGLKLAAYQLL
                     RTDLGDDPVLVLDDVFAELDTGRRERLAAMVADCEQVIITAAVPADVPTDLVGRTFSV
                     TLGSVSERD"
     gene            complement(7044..8114)
                     /gene="gnd"
                     /locus_tag="PL014_07125"
     CDS             complement(7044..8114)
                     /gene="gnd"
                     /locus_tag="PL014_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HOT56897.1"
                     /translation="MQLGLVGLGKMGGNMRERIRRAGHEVVGYDRNKAVSDASSLADM
                     VRQLDAPRVVWVMVPHGAPTRDTVAELAELLDKGDLVIDGGNSKFTEDFENEKLLKAK
                     GIGYLDCGVSGGIWGLDNGYGLMVGGTAANVKKAMPIFDALRPEGPRDEGFVHAGKVG
                     AGHYTKMVHNGIEYGLMHAYAEGYELLEKKDIVENVPGAFKAWSRGTVVRSWLLDLMV
                     KALEENPGLADVSDYTTDSGEGRWTVEEAINLGVPMPVISASLFARFASRQQVSPTMQ
                     AVAALRGEFGGHQVMTTAEGEALRTGVPAEAGAAAATKKAAAKEKAPRTAKAAAAKGR
                     EAAAAGPSSGAGSTRKRAAKKS"
     gene            complement(8179..9312)
                     /gene="dnaN"
                     /locus_tag="PL014_07130"
     CDS             complement(8179..9312)
                     /gene="dnaN"
                     /locus_tag="PL014_07130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT56898.1"
                     /translation="MKFRVERDVLAEAVTWVARGLPARPPVPVLAGILLHAEDDGTLT
                     LSAFDYEVSARITVAAEVSEPGTVLVLGRLLADISRNLPDRPIDVVTEGAKVQVTCGS
                     SRFSLLMMPADDYPTLPSAPQPSGSIDGHLFTQAVAQVSVAADRGDTLPILTGVRVEV
                     EGDTMTLLATDRYRLAMRELHWNPTATDASHVVLIPARTLSDISRSLGASGSVDVALG
                     TAAGGDGLVGFEAGQRHTTTRLLDGEYPKVASIFPSSSETESVVRTSDLVEAVKRVAL
                     VAERNTPVRLRFSEGQVAIEAGTGDDAQASEAVEATLTGPEIEIAFNPTYLLDGLGAV
                     GTPFSRISFTQASRPAVLTGQVEEDGEADTSYRYVLMPVRFAS"
     gene            complement(9972..11534)
                     /gene="dnaA"
                     /locus_tag="PL014_07135"
     CDS             complement(9972..11534)
                     /gene="dnaA"
                     /locus_tag="PL014_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOT56899.1"
                     /translation="MVHLWRTTLEVLDSDGIPVQQRAFLSLAKLVGLIDDTVLIAVPN
                     DFTKEIIETRLRDRVAATLGQQLGRDVRLAVTVDPSLADAPPTTDGHDTSSTAAAPEG
                     VDDGHDRRAAGRRQQIVDLDIGEDEVDAGDGHGHLGDHGAADAAPGPNAIGIPDLVEV
                     PGARRPRPHTQVPELVELTRLNPKYTFDTFVIGASNRFAHAAAVAVAEAPAKAYNPLF
                     IYGDSGLGKTHLLHAIGHYARNLFPHVKVRYVNSEEFTNDFINSIRDDKAANFQRRYR
                     DVDVLLIDDIQFLQGKVQTQEEFFHTFNTLHNANKQVVITSDVPPKLLSGFEARMRSR
                     FEMGLLTDVQPPDLETRIAILRKKAIQERLSVPDDVLEFIASRISTNIRELEGALIRV
                     TAFANLNRQPVDMSLAEIVLRDLMPDDASRQVTPATIMAQTAAYFGLTLDDLQGQSRS
                     RVLVTARQIAMYLCRELTDMSLPKIGQQFGGRDHTTVMHADKKIRQLMAERRAIYNQV
                     TELTNRIKQQSN"
BASE COUNT         1620 a         3800 c         4254 g         1836 t
ORIGIN      
        1 ccttgggggc gatcgtcatg tcagccagcc tttctcagtg gtcccaggcg atgacgatct
       61 cgtcgtcatc ccattccttg atgtgcccga aatagctcac gatggcgagc tggaactcgt
      121 gggtctccca ctcgcggccg aggcgttcct cgaccgactc acgggtgacg acgaggcgac
      181 cgggtgcggt gatccgcacg agccccggca tgtgctgaac cgtgcagccg tcgttcgtgg
      241 cctcgatggc ctcgacgacc gagcggttgg cctcgctctc ctggatgtcc acaccgacgg
      301 tgcgggtgga ggcggtggtg ctcatcgtgt cgtccttccc gggtcaggcg gtcagcgccg
      361 cgacggcgtc acggacggtg gtggcggtgg cctcgacgtg ggcggctgca ccggcgccga
      421 cgagctcgtc ggcacgggca gcgagcgggg cgatggctgc ctgtgcggct gcgagcacct
      481 cggtcgtggt ggccgcgagg agcgccttgt tcgcagaacc cgtctcgggg tcggccaccc
      541 atgccttgta cagggcgtcg acccacttgc gctggtcggt gaaccacgac gcctggtgct
      601 gggcgagcag gctgtacgac ccggcgccgg cgtcgatggc ggcatcgtcg aggtgaccga
      661 agaacagccc gtagatgagc cggtcgagca cgtcgaggcg gatgagggcc ttgccccagt
      721 ccttctcgag gaagagctcc tccatgtagc ggcgcagccc ctgcagcgag gcgtcctcga
      781 cccatcgggt cttggcctcg ccgagcagtg cggcggtgcc accgtcgagg gcgatcccga
      841 tacggctgac ggcctgggcg ttgccgatcc ggtcgaaagc cgcataggcg gcgcactggg
      901 tgatcgtcgt gccgaagccg aagcgcgcga tgtcgcacga gaccatctgg gcgcccgact
      961 cgtagtgacg cagcggcacg aggagctcac ccatgagcgc ggaccaggcg tccggcatcc
     1021 gggagaggag gtcgcgcttg tcgaggtagt cgagcgtgga ggccacggca tccgccatcg
     1081 ccgcccggga ggtgacgtac ggggcgtagt agtactggcg cgggtccgtg aacgagtacg
     1141 ggtcggtgag ccggaacgcg ctgaacgcct cgtcgtagat gtcgtgggcg gggtcccaca
     1201 ggggccggta gtggaagttc tccttcggct ggatgccgat ggagccctcc tcgtagcggg
     1261 atgccgggcg gtcgccgtag cgctcgacga ggtgggtgaa cgtctttcgt ggcggctcga
     1321 tgacctgggt tcggagctcg tactgcacgg tgcctcctcg gggcgggtcc tgcggatcgg
     1381 tgtgacgagg tggtcgaagg tggtgggctg ccgcgagtgg ggcggtcgcc ggtgcggttc
     1441 ccgggccggt ggctggtgcg gccggtcagg gaacgtggtg gtggctgacg cccgactcca
     1501 cgagcaggcg cacgatgagg tcgggcaggg cgtcgggccc gtcgagctcg tggggaagct
     1561 cggactcgaa gccgtcgccg acaagacgcc cgtatgccgt gccgtcctcg aacgtcgtcg
     1621 acacgcgggc cgctcgcccg gcatgggtca cgctgaaggc cagctcgtgc gggagggtct
     1681 cgtcgacgag ctggagccgg tcgtcggcct ggccgaggat ggcgttggcc tcgctgacga
     1741 gctgctcggc gagcgagcgg atggcgacct ggcggtcggc tccggtgccg gtggcgatcc
     1801 gccggctgat ccgggtctcg ggcatcgggg cgagctcgtc gagctcggga agctggtcct
     1861 gcggggtgcc gacgatctcg gcgacccgga gcatgaacgg ggtcatgggg tgtcctctcc
     1921 gtgcgtgagt gcggcggcga cggcatccgc gccagccgtg gcgacctgct tgtcctggac
     1981 ggccgagccg ccggaggcgc cgaccgcccc gacgagcgca ccgtcgacga gcacgggaac
     2041 cccgccgccg aaggcggcga cccggccacg ctgggcgatg ccctcgcgca cggcatcctc
     2101 attcgagatg gcctcgtaga actcgtcggt ggggatgccg ttgaagccgg ccacggtcca
     2161 tgccttgtcc tgcgcgattc cgccggagag ggcgaaggcg ccgtccatcc gggcgaaggc
     2221 gagcaacccg ccggtgctgt cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnngcgtgcg ggcaggggcc atcgcggtga cggcggccgg ttggtgggcc gccgtcaccg
     2401 tgacgacggg gtcgatcagg tcaggaccga ggtgaacgcg tcgttgagct cgcgagcgtg
     2461 gtagaacacg cccttgccca gcgcgtcagg ggtccagacg ttcatcgggc ggtccgggta
     2521 ggccaggtag ccgccggcga acacctcgtt gcggttgccc gaggggtcga agaagtagac
     2581 ggtctggcca cgggtgatgc cgtgacgggt cgggccgacg tcgatcggca cgtcgtccat
     2641 ggagaacagg tccgcggcgc ggccgacctc ggtccagtcg ccgaggcgga aggcgaagtg
     2701 gtgcagcttg ccctgggggc cggcgagcac ggcgagctgg tggatctggg tgctgttcgt
     2761 catccaggtc gcgatgatgt cggcgtcgtc gtcgaggctg gtctgcacgc gctcggtggc
     2821 gtagaagccg aagacgtcgg tgaggaagcg gtccatcatg ccggggtcct cggcggtgat
     2881 gagcgagtgg tccagggcgg ggaccccgac gccgacgagg tggcggggga aggagtccgg
     2941 gttgtgcgtg ccgacctcgg tgccgatgag ggtggcctcg tggtagacct cgaagacgtg
     3001 gtcggagggg gtggtgaacc ggatgccgtc gccgatctcg gggttctcgc ccttgctcat
     3061 ccgctcgacg gcgacgccga aggtctgggc cttcttctcg atctcgtcga tgtcggagga
     3121 gtactgcacc ttccagccgt acttgaccag gccgacgccg ccctcctcga ggacgaccga
     3181 gtggtggtcc cactcatccc acgccttgta gtagaccttg ttgccctcgg agagggtttc
     3241 gtacaggccc atcgtgttgg cgtagtggtc cttggcctcg gccaggtcgg tgacgcggac
     3301 gtgggcgtag cccaacctca tgactgccat tactgctcct tgggggtggt gccggccatc
     3361 aggcccgcgg ctgcgtgggc ctcggcgcgc tgccgggcct tgtcgttgat catgtgcagg
     3421 aaggtgttga cgagacggac gttctcgccc cgcagctcga tcgtgatgtc ggtgtcgggc
     3481 acggcccggc acgtcaggca ggtgccgtcg aggtgttcgt cctcggtgag caccgtgtcg
     3541 gcgaccgggt ggtcgtagcg gaactgaccc gagcggcagt cgaccttgca gatcgcacag
     3601 ccgccgcgac ggcatcccac cgtgtacgcg aaacccgcct tgtacagacc gctgagcacg
     3661 gtctctccgg gctcgaggta gatgacctcg cccgtggggt ggatcgtcac ggtcggcact
     3721 gccgctccct tgcgctcagt ggtggcgacg ccatgtcgct cacattgaga gtcgcggggt
     3781 gcggggggtg ggaccaccca cgttccgtgg agaggaacgt cagcggcgca gcgcggcggc
     3841 cattcgctgc tgggcgacct ccgagtggaa gagcagcccg gagagccgcg ccatctcgtc
     3901 gccgtggctg tcgaactgcg cgacgaggtc ggtggtgagc aggcgcttgg cctcgaccag
     3961 gccctgccgg gcacccgcgc gcaggtggtc gagcacgcgc gcgacgacgg catccatctc
     4021 gtccgcgctg gcgacgtgcg tgaccaggcc ctggtctcgg gcctcggtgg cgctcacgct
     4081 gcggccggtg aggaaccagt cggatgccgc gcgcgggctg aggcgggcgc gcagcgggat
     4141 ggagatgacg gcggccgcga ggcccaggcg gacctcggtg agcgcgaccg tgaccgagtc
     4201 ggcacacacg acgatgtcgc tggacgccac gagcccgagg cctcccgcgc gcacgggccc
     4261 gtcgagccgg gtgacgaccg ggaccggcgc ggcgacgatc gcgcgctgga gggcgatgat
     4321 gccgcgcgcg gactcgacca tgtcgacggt cgcggcctcc ttgaggtcgg cgccggcgca
     4381 gaacaccggg tgcgagctgc gcaggaggat gccgtgcagc gactcgtcgg cgcccgcctg
     4441 agcgaggtgg tcggtcagct cggcgatgag ccgccgagag agggcgttgc ggttggccgc
     4501 ggagtcgagg gtgatgacgg cgatgaggtc gtcgtcggcc cggtgcacca gtgcgtcgct
     4561 cacgctccga ccctagcccc gacttcttgc actccagcca ccgcaccacc cgcgacttct
     4621 tgcaccccag ccacctcagc ggccgtcggc gagccgcgcc aggtgggccc gcaccacggc
     4681 gtcgaccagc tcggtcggga agggcttctt gtagagcagc ttcagcgtgc tggcacctga
     4741 gctgtgggtc gccaacgccg ccgtggtctg cgggtcgagg tcgcgctcct ctggcagggg
     4801 gtagatcgcc aggtgcgcct tccagggagc ggcgtgcagc agacgcttgc cgtcgacgag
     4861 gaagacgggc atcgagtacg agatctcctc ggtcgcctcg ggtgcatggc gctgaacgat
     4921 gtcgcgcagg atgccgtaga actcacgccc cgcgtcggtt gccgacgcga gatggtgcgc
     4981 cacggcatcc ggcaccggtg gtcgcgacgt ggatgccgga gtctcgtctg gcgtcgtcgg
     5041 ctgatctgac gctggtcctc ggcatccggg ggagtcagcc atgcccgtca tcctgccccc
     5101 gcctcgcccg acttcgtgcg acttgcgtgc ggttgcgggg ccacaacggg cgattcagcg
     5161 caccgaagtc gcacgaagtc ggggaggttc aggtggctgg ggtgcaagaa gtcgcgggtg
     5221 ggtcagccgt aggtgtctcg tgggccgcgt ccgtcgttgg cccggcgctg cccgcgcgac
     5281 cacgacggtg cccccggccc gagcactcga agctcgctga ccgtgccctc accgacgtcc
     5341 tcggccaggc gcgccagcag gctcgaggag agcagccgca gctgggtggc ccacgccgtg
     5401 gagtcggccc gcacggtgag cacaccgtcc tcgaagacca ccggggcaca gtgcagcgcg
     5461 atctccgggc cgacgatctg cggccaccgc ccgatcaccg ccccgacggc cacgtcgacg
     5521 gcccatcccc gctcgacgac gaactggtcg agctgctccc cgagcagctt cgggtcgcga
     5581 ccggtgctcg tgcgctcgcc gaccggcacg tccttgaccc ggcgacgcgg gcgcagaccc
     5641 ggccgcaacc ccttggcctt ggcggcggcc cgcgccctgg cgagcgcagc ggcggcagcc
     5701 tccccggcat ccccgatcgt gaggtgttcg ggctcatcgg agatcggggt cgactctgcc
     5761 ggggaagcac tctcctcgct ggacgagcgt tctccaggag agtgcggctt catcgggtca
     5821 cccgatacgg cgtcagtcac gttcgctcac cgaccccagc gtcaccgaga acgtgcgccc
     5881 caccaggtcg gtcggcacgt cggcgggcac cgcggcggtg atgatcacct gctcgcagtc
     5941 cgccaccatc gcggccaacc gctcgcgccg cccggtgtcg agctcggcga acacgtcgtc
     6001 gagcaccagc acggggtcat cgccgaggtc ggtgcgcagc agctggtagg ccgcgagctt
     6061 gagccccagc gcgaacgacc agctctcgcc gtggctcgcg taccccttgg ccggcagctc
     6121 accgagcccc aggatcacgt cgtcgcgctg cggcccgacg aggcacaccc cccgctcgat
     6181 ctcctgcgcc cgcacctcgg ccagggtcgc gagcatctcg ctctcgagct cgtccacctc
     6241 gggcacctcc ccggccgcga tccgctcggc ggtctcggcg cgcagcgacg acctgtacgt
     6301 cagcgtcgcg ttcgccggcc cggcactgac ctcgtcgtac gcggccgcga ggaacgggcc
     6361 gaggtcacgc agcagccgca gccgtgcgta cagcaggtgc gacccggcac cggccagctg
     6421 ggagtcccag acatccaacg tgtgcagagc cgaatcacgt tgtgccgcag cctcactgcc
     6481 cagctcggat gccgtccgcg cacctcggcg accacctcgc cgcagcacgg gggcggcgga
     6541 cttcagcagg gctccgcgtt ggcgcaggat cttgtcgtag tccgagcgca ccccggccca
     6601 gcgtggctgc cgggccacga gcaggtcgtc gaggaagcgc cggcgctcac cgggatcacc
     6661 cttgaccagg gcgaggtcct ccggggcgaa gagcacggtc cgcagggtgc ccagcacgtc
     6721 ccgcgggcgg gtcagcggtg agcggttcag cctggcccgg ttggcccggc cggggttgat
     6781 ctcgagctcg accatgctct cgcgcccgtc gcgcacgact gcaccgcgga tgacggcctg
     6841 gctcgcgccg aaccgcacga gcggggcgtc ggtggcgacc cggtggctcg acaacgaggc
     6901 caggtacccc agggcctcga cgaggttggt cttgccctgc ccgttcaggc caaccagcgt
     6961 ggtcaccccc ggctcgagcg gaagctcggc cgaggtgtag ctgcggaagt cgacgacgct
     7021 caggtgccgg acgtgcaccc gggtcagctc ttcttggcgg cacgctttcg cgtggatccc
     7081 gcaccggacg acgggccggc cgcagcggcc tcgcgaccct tggcagcggc cgccttcgcg
     7141 gtgcgcgggg ccttctcctt ggccgccgcc ttcttcgtgg ccgcggcggc accggcctca
     7201 gccggcaccc cggtccgcag ggcctcaccc tcggccgtgg tcatcacctg gtgcccaccg
     7261 aactccccgc gcagggcggc gacggcctgc atcgtcgggg agacctgctg gcgggaggcg
     7321 aaccgggcga acagcgacgc ggagatcacc ggcatcggca cgccgaggtt gatcgcctcc
     7381 tcgacggtcc agcgaccctc gccggagtcg gtggtgtagt ccgagacgtc ggccaggccc
     7441 gggttctcct cgagcgcctt gaccatgagg tcgagcagcc aggagcgcac cacggtgccg
     7501 cggctccacg ccttgaacgc ccccggcacg ttctcgacga tgtccttctt ctcgagcagc
     7561 tcgtagccct cggcataggc gtgcatcagg ccgtactcga tgccgttgtg caccatcttc
     7621 gtgtagtgcc cggcccccac cttgccggcg tgcacgaacc cctcgtcgcg cgggccctcg
     7681 gggcgcagcg cgtcgaagat cggcatggcc ttcttcacgt tggcggcggt gccacccacc
     7741 atcaggccgt agccgttgtc caggccccag atgccgccgg agacaccgca gtcgaggtag
     7801 ccgatgccct tggccttgag cagcttctcg ttctcgaagt cctcggtgaa cttgctgttg
     7861 ccaccgtcga tgacgaggtc gcccttgtcg agcagctcgg cgagctcggc gacggtgtcc
     7921 cgggtcggtg caccgtgcgg caccatcacc cacaccacgc gcggagcgtc gagctgacgc
     7981 accatgtccg cgagggacga cgcgtcggag accgccttgt tgcggtcgta cccgacgacc
     8041 tcgtggccgg cgcggcggat ccgctcgcgc atgttgccac ccatcttgcc cagaccgacc
     8101 agacccagct gcatgtcgtg actcctcgtg gtgatggtgg tgatgggggt gacgcgtgtc
     8161 ctgcggggtg ccgcgacctc agctggcgaa ccgcaccggc atcagcacgt agcggtagga
     8221 cgtgtcggcc tcgccgtcct cctcgacctg cccggtgagc acggccgggc gcgaggcctg
     8281 ggtgaacgag atgcggctga acggggtgcc gacagcaccg agcccgtcga gcaggtaggt
     8341 ggggttgaag gcgatctcga tctcagggcc ggtgagggtg gcctcgacgg cctcggacgc
     8401 ctgggcgtca tcgccggtgc cggcctcgat cgcgacctgt ccctcgctga agcgcagccg
     8461 cacgggcgtg ttgcgctcgg cgacgagcgc cacgcgcttg accgcctcga cgaggtcgct
     8521 ggtgcgcacg accgactcgg tctccgagct ggacgggaag atcgacgcga ccttcgggta
     8581 ctcaccgtcg aggagtcgcg tggtggtgtg ccgctggccg gcctcgaacc cgacgaggcc
     8641 gtcgccaccc gccgcggtgc cgagggcgac gtcgaccgaa ccgctggccc cgagcgagcg
     8701 cgagatgtcg gacaacgtgc gcgccgggat gagcacgacg tggctggcat cggtcgccgt
     8761 cgggttccag tgcagctcac gcatcgcgag gcggtagcgg tcggtggcca gcagggtcat
     8821 ggtgtcgccc tcgacctcga cccgcacccc ggtgaggatc ggcagggtgt caccacggtc
     8881 ggcggccacc gagacctggg ccacggcctg ggtgaacagg tgcccgtcga tggacccgct
     8941 gggctggggc gccgacggca gcgtcgggta gtcgtcggcg ggcatcatca gcaggctgaa
     9001 ccgcgacgag ccgcacgtga cctggacctt tgcgccctcg gtgacgacgt cgatcggccg
     9061 gtccggcagg ttgcgtgaga tgtcggcgag caatcgcccg agcaccagga ccgtgcccgg
     9121 ctcggagacc tcggccgcga cggtgatgcg ggccgagacc tcgtagtcga acgcggagag
     9181 ggtcagcgtg ccgtcgtcct cagcgtgcag caggatgccg gccagcaccg gcaccggagg
     9241 acgtgcggga agaccgcggg ccacccaggt gaccgcttcg gccaggacgt cacgctcgac
     9301 gcgaaacttc acggcatacc acctcgtccg gcgcgtcggg cgcgcccttg ctcgtgcaca
     9361 gatgtgaccc gccccggtga gcggtcgcgc ggtgcgcgcg gtgccgatcc ggacggggaa
     9421 accgagacta gaccacccac ggcacctgcg cccctgcgaa cccctgaaat acccgctgtc
     9481 cgggcacccg atgacggatg cgggttgtca ttcggatgtc gcgtgggatg ccgttcgggt
     9541 cgttgtcggg gccgagatga gttccagatg tctcatcgtt gtcataggtc ctgtggatcc
     9601 tgtggacggc tgcccatcac cgcaggtcac ctcggcgatc cggggtgtgg accagtcgtg
     9661 gacgaggagt ggacgacggt gggtggccct gttgacaccc acggctcgtt cacagggcgt
     9721 ccacgtcgac gacgcgcgtc gtccacgtcg agggggtggc ttgtccaccg gcatccgagg
     9781 ttttccacag gcgttgtgga caacccggca tgcgacagca tgaactgagg aggaagcggg
     9841 gtgcacgaga aggggttttc cacaggtttg agcacagcgg tggatgaaat cgagccgtgt
     9901 cgagcacgtt cgccccggtg cggtcctcca cgagcggtgt ggacagcctg tggacaacgc
     9961 cggtgcagga gtcagttcga ctgctgcttg atccggttgg tcagctcggt gacctggttg
    10021 tagatggccc tgcgctcggc catcagctgg cggatcttct tgtccgcgtg catcacggtg
    10081 gtgtggtcgc ggccgccgaa ctgctgcccg atcttcggca acgacatgtc ggtgagctca
    10141 cggcacaggt acatggcgat ctggcgggcg gtgacgagca cccgggagcg ggactggccc
    10201 tgcaggtcgt ccagcgtgag cccgaagtag gccgccgtct gggccatgat cgtcgccggg
    10261 gtgacctggc gcgacgcgtc gtcgggcatg aggtcgcgca ggacgatctc ggccaggctc
    10321 atgtcgaccg gctggcggtt gaggttggcg aaggcggtga cccggatcag cgcgccctcg
    10381 agctcgcgga tgttggtcga gatgcgcgag gcgatgaact cgagcacgtc gtccggaacc
    10441 gacagccgtt cctggatggc cttcttgcgc aagatcgcga tccgggtctc gaggtcggga
    10501 ggctggacgt cggtgagcag acccatctcg aaccggctgc gcatcctcgc ctcgaagcca
    10561 ctgagcagct tgggcggcac gtcactggtg atgacgacct gcttgttggc gttgtgcagg
    10621 gtgttgaagg tgtggaagaa ctcctcctgc gtctgcacct tgccctggag gaactggatg
    10681 tcgtcgatga ggagcacgtc gacgtcgcgg tagcggcgct ggaagttcgc ggccttgtcg
    10741 tcgcggatgc tgttgatgaa gtcgttggtg aactcctcgg agttcacgta ccgcaccttg
    10801 acgtgcggga agaggttgcg tgcgtagtgc ccgatcgcgt ggagcaggtg ggtcttgccc
    10861 agcccggagt ccccgtagat gaacagcggg ttgtacgcct tggcgggtgc ctcggcgacg
    10921 gcgacggcag ccgcgtgggc gaaccggttg ctcgccccga tgacgaaggt gtcgaaggtg
    10981 tacttggggt tcagccgggt cagctcgacg agttcgggca cctgggtgtg gggtcgtggt
    11041 cgccgcgccc ccgggacctc gacgagatcg gggatgccga tggcgttcgg tccgggggcc
    11101 gcgtcggcgg cgccgtggtc accgaggtga ccgtgcccat cccccgcgtc gacctcgtcc
    11161 tccccgatgt cgaggtcgac gatctgctgg cgccggcccg cggctcgacg gtcgtgcccg
    11221 tcgtcgacgc cctcgggagc ggctgcggtg gaggaggtgt cgtgcccgtc ggtggtcggg
    11281 ggtgcgtcgg ccagggaggg gtcgaccgtc accgccaggc gcacgtcgcg gccgagctgc
    11341 tggcccaggg tcgcggcgac ccggtcgcgc agccgggtct cgatgatctc cttggtgaag
    11401 tcgttcggga cggcgatgag cacggtgtca tcgatcaggc cgacgagctt ggcgagggag
    11461 aggaaggcac gctgctggac ggggatgccg tcggagtcaa ggacctcgag ggtggtgcgc
    11521 cacaggtgca ccatcgtcgc gtccgccact tgctcccaca atccttccga tgaccgggtc
    11581 gatgccggtc aggtttcccc gaggtcgtcc
//
LOCUS       DAOSOC010000074        11489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5608_length_11489_cov_7.859192, whole genome shotgun sequence.
ACCESSION   DAOSOC010000074 DAOSOC010000000
VERSION     DAOSOC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5608_length_11489_cov_7.859192"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..643)
                     /locus_tag="PL014_07140"
     CDS             complement(23..643)
                     /locus_tag="PL014_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOT56900.1"
                     /translation="MFDHVEVTGTGSASAVPDVVVLDARVSCDAPDVAGALAKASERV
                     SAALAAASEAGVAAADRQSTGMGVNSRWDHEGRGVIGYSAYQSLRLLVRDRDGVGDLI
                     SALARAAGDSFGLDGVRLEVAEKAPLVEQARAAAFADAVRTATQYAQLAGRPLGQAVK
                     VIEGGSGPGPAPKMRAMAAMDSVGGMPIEAGEHTVTVTVAVRFGLG"
     gene            722..2131
                     /locus_tag="PL014_07145"
     CDS             722..2131
                     /locus_tag="PL014_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOT56901.1"
                     /translation="MPSSPSIANSVTVRLTLPARATAVSEMTGVIEKAGGVVTGLDVT
                     ASGATKVRIDMTMLTHDPEHADAIVAVMREVEGVEIGRVSDRTFLMHLGGKLSVESKV
                     PIRTRDDLSMVYTPGVARVCLAIAENPADARRLTIKRNTVAVVTDGTAVLGLGDIGPL
                     AALPVMEGKAALFKRFADIDAFPICLDTTDTEEIISIVKAISPAFAGINLEDISAPRC
                     FEIEARLRAELDIPVFHDDQHGTAIVALAALRNALRVVGKRLEDVRLVMSGAGAAGTA
                     ILKLFLHAGAQHVVVADVDGVVHRGRADVLSGEHPNHAWIAANTNPDDVTGTLSEAMR
                     GADVFLGVSAPDVITEADVESMAPDSIVFAMANPVPEIDPDIAARHAAVVATGRSDFA
                     NQINNVLVFPGVFRGLIDSGVSHVDMDVLLAAADALADVVRDDERNATYIIPSVFHPD
                     VSTVVADAVGAAVAAREGA"
     gene            2128..2469
                     /locus_tag="PL014_07150"
     CDS             2128..2469
                     /locus_tag="PL014_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOT56902.1"
                     /translation="MTPAHRRRVLLGLLVAAVFAQFVVLYAPAQPGGLPVFAHADKVV
                     HVLVFLVPTALALVAGLPARVVVPLFAAHAVVSEVVQGALLPMRAGDPFDVLADLTGV
                     ALGVLLWRLRR"
     gene            2748..>3479
                     /locus_tag="PL014_07155"
     CDS             2748..>3479
                     /locus_tag="PL014_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT56903.1"
                     /translation="MPKKATGPKKARWTTAQKFEAKKAPKKAHHRGQPDGPKTAPKRA
                     ARAESGEYTKRPRAERSPRWDREDPRRQATAGRPDSRGGDPRGGSRGDTRSDSRGGDR
                     PYRRDDRGHGRDDRGSSTRGDSRGQRYSSGQSDRGYTSNREDRNDRPKRWVSDSREER
                     HVHRPHDERPRLERGERPVRDRAPRVADPARRDRHEGRRVERDADRGGYQGRDDRGGS
                     RGGYQGRDDRGGYQGRDDRGGSRGGY"
     assembly_gap    3480..3579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3580..5107
                     /locus_tag="PL014_07160"
     CDS             <3580..5107
                     /locus_tag="PL014_07160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT56904.1"
                     /translation="SRGGYQGRDDRGHRGAPQVDDDRPLSFEEAEAQRAEADTWTHYS
                     STAGAAKEVTADNGFAALGVPAVLVERLARDGIADPFPIQEATIPDAIAGRDVLGRGR
                     TGSGKTLAFGLSMITRLADGSRPQSKRPRSLILVPTRELAMQVSDALEPFVHVTGLRH
                     KLVAGGLSYEPQIKALAKGLDILVATPGRLADLMERGAVDLSAVEIAVLDEADHMADM
                     GFLPEVTAILDTIPQGGQRLLFSATLDKGIDTLVDRYLVDPVTHSTDDAQASVTTMSH
                     HVLLIDPQDKKAMTAELAGRGGRVIVFARTQLGSDRIAGELRERGVLAASLHGGLSQA
                     VRNRVLGAFRDGSMNVLVATDVAARGIHVDDVGMVVQVDPPTDHKDYLHRAGRTARAG
                     EEGAVVMLALPHQRKMVTRIIEGAGAEAKTAKVAPGDATVQAMGGRAPSGVPVPDAVW
                     RPILEGGKNGGRRGGGRPGGRPAGRPGGRPGGRPSHSGGGHSRHGSGRPAGGRGRMDG
                     "
     gene            5104..5664
                     /locus_tag="PL014_07165"
     CDS             5104..5664
                     /locus_tag="PL014_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOT56905.1"
                     /translation="MSTSDTTGKLLVATPQIEEGVFARSVVLVLHHDDDGAQGLVLNR
                     PVDADIDAVLPGWEAHASSPAKVFQGGPVQLDSALGLAVVPGDAPAPSGVRRLFGSVA
                     LVDLDAQPSLVVPAVAGLRIFAGYAGWSPGQLEDEIRTGSWFVVDSESGDVFTDEPAG
                     LWQRVLRRQGDPLRWVAWYPRDPSLN"
     gene            complement(5670..6911)
                     /locus_tag="PL014_07170"
     CDS             complement(5670..6911)
                     /locus_tag="PL014_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOT56906.1"
                     /translation="MTTSEPREAYVWIWLPGATDPVVAGLLEAQSNGIVTFTYGRSYL
                     NRSDAIALYLPELPLRPGRIEPLPGLSLPSAIRDAGPDSWGQRVILARRTGHLTRHSD
                     NGDLGPLTYLLESGSNRIGALDFQSRPDVYVHRGGEATLDQLVSAAQALDAGEALPEE
                     LGEALVRGTSIGGARPKALISDGKRHLVAKFSSASDTYPVVKAEALGMLLARAAGVRV
                     PNVDVTRSLGRDVLLVERFDRTTMADERRQMVSALTILGLDEMMARYATYTDVADVVR
                     ARFSDPAGTLAELFRRIAVNICIGNTDDHARNHAAFWDGHTLALTPAYDLCPQPRSGR
                     EANQAMAFGPNGQRAARLTLLLESAPTYLLSQRAARNILDEVVNGIRNSYRDCADQAR
                     LTTEEASRLMGRQILNPYAIE"
     gene            complement(6898..7233)
                     /locus_tag="PL014_07175"
     CDS             complement(6898..7233)
                     /locus_tag="PL014_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT56907.1"
                     /translation="MAKHALPYSAPTVLAAELLGQQVAKGRRERGWTVAELAERVGVH
                     PVTITKIERGAPTVALGTALEAAVLCGVTLFGRDEPSLVAEVRHGRETLALLPTRVRP
                     REEPVRDDF"
     gene            7386..7688
                     /locus_tag="PL014_07180"
     CDS             7386..7688
                     /locus_tag="PL014_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HOT56908.1"
                     /translation="MSETMPPLDDPNAPAGQPATRTAVLERTEAVPQVAEPGDHERFS
                     HYVRKEKILESALSGEPVTALCGKVWVPGRDPKKFPVCPTCQEIYDGLRAPQDGDK"
     gene            7833..>9618
                     /locus_tag="PL014_07185"
     CDS             7833..>9618
                     /locus_tag="PL014_07185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT56909.1"
                     /translation="MSTSAAGHLSPAFPERAAWGTASKLRAWQEEALASYLRSESRDF
                     LAVATPGAGKTTYALRVATELLDRGIIRAVTVVAPTEHLKTQWADAAARVGVHLNPAF
                     SNSSGVQSHEYHGVALTYAQVAANPALHRRRTIDTPTLVILDEIHHGGDAKSWGDGIR
                     EAFEPATRRLSLTGTPFRSDTSPIPFVRYEMGEDGILRSASDHTYGYAEALRDGVVRP
                     VLFLAYGGAMRWRTKAGDEVSARLGEPLTKDLTAQAWRTALDPKGEWVPSVLAAADKR
                     LSEVRRHVPDAGGLVIASNQTAARAYAKLLQGITGVAPTVVLSDDAGASTRIEKFAAD
                     TSRWMVAVRMVSEGVDVPRLCVGVYATSTSTPLYFAQAVGRFVRARKRGETASVFLPS
                     VPVILSHAAAMEVERDHALDRKTTAEGEASLWAEEEGLLAAANRTETEAELEDSSFEA
                     LESDAHFDHVLFDAQQFGLHAAVGSQEEQDYLGLPGLLEPEQVTTLLNERQARQSKSA
                     TRRGGAPAGGAATASGPSVVSAHRALASQRKELNKLVAAYSSKTGAAHAAIHMDLRRT
                     CGGPELASATSEQVTERIDRIRRWFVGRR"
     assembly_gap    9619..9718
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9719..10188)
                     /locus_tag="PL014_07190"
     CDS             complement(<9719..10188)
                     /locus_tag="PL014_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT56910.1"
                     /translation="MPRQTGIDDLDARLIALFTEQPHVGVLGASRTLGVARGTVQARL
                     DRLEERGVITSYAPHVSSEALGYPVTAFCTLEIRQRQGHAPVVAHLSAIPEVLEIHSI
                     TGVGDLIVRIVARDNADLGRVIDEIIDDTHVLRANTAICLVTHLDHRTGPLVEAA"
     gene            10297..>11489
                     /gene="hppD"
                     /locus_tag="PL014_07195"
     CDS             10297..>11489
                     /gene="hppD"
                     /locus_tag="PL014_07195"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOT56911.1"
                     /translation="MTDTTMTPASHIDLTEQERQAGLDLQQLKQLVGLVDYDEQQDPF
                     PVTGWDALVFVVGNATQAAHYYQSAWGMELVAYAGPEHGQRDHKAFVLKSGSIRFVLK
                     GAVSPDSPLIAHHAAHGDGVVDISLEVPDVDRCIEQARRAGATILQEPETVSDEHGSV
                     RIAAIATYGETRHTLVQRAVDGVTYAGPYLPGYVAAASTYVKRDGAPKRLFQALDHVV
                     GNVELGKMDEWVGFYNKVMGFVNMAEFIGDDIATDYSALMSKVVANGNHRVKFPLNEP
                     AIAARKSQIDEYLEFYAGPGAQHLALATNDIVRTVDELRKEGVEFLDTPDAYYDDPEL
                     RARIGEVRVPIEDLKSRGILVDRDEDGYLLQIFTRPLGDRPTVFFEIIERHGSLGFGK
                     GNFKAL"
BASE COUNT         1666 a         4108 c         3859 g         1656 t
ORIGIN      
        1 gcgtcgaccc accccacggt gctcagccca gcccgaagcg gaccgccacg gtgacggtca
       61 cggtgtgctc gcctgcctcg atcggcatgc caccgacgga gtccatcgcc gccatggcgc
      121 gcatcttggg tgccgggccc gggccgctgc caccctcgat gaccttgacc gcctgcccca
      181 gggggcgccc cgcgagctgc gcgtactgcg tggccgtgcg gacggcatcc gcgaacgccg
      241 cggcgcgcgc ctgctccacc agcggtgcct tctccgcgac ctcgaggcgc acgccgtcca
      301 gcccgaagga gtcgccggcc gcccgcgcca aggcgctgat caggtcgccg accccgtcgc
      361 ggtcacgcac gagcaggcgc aggctctggt acgcgctgta gccgatgacc cctcggccct
      421 cgtggtccca ccgcgagttc acccccatgc cggtgctctg ccggtcggcg gcggccaccc
      481 cggcctcgct cgcggcggcg agggctgcgg agactcgctc cgacgccttc gccagcgcgc
      541 ccgcgacgtc cggggcatca cagctgaccc gtgcgtcgag gacgacgacg tcggggacgg
      601 cgctcgccga accggttccg gtcacttcca cgtggtcgaa catgggcgtc actgtaggcc
      661 gaccggatcg acatggacgc agattccgat ctacctgtcg gtaggaggtt caatcaccgg
      721 tatgccgtcg agcccctcca tcgccaactc cgtcaccgtc cgcctcaccc tcccggcgcg
      781 cgcgaccgcc gtgagcgaga tgacaggcgt catcgagaag gccggcgggg tggtcaccgg
      841 actcgacgtc acggcatccg gcgcgaccaa ggtgcgcatc gacatgacga tgctcaccca
      901 cgaccccgag cacgccgacg ccatcgtcgc cgtgatgcgc gaggtcgagg gtgtcgagat
      961 cggccgggtc tcggaccgca cgttcctcat gcacctcggc ggcaagctgt cggtggagtc
     1021 caaggtaccg atccgcaccc gcgacgacct gtcgatggtc tacacgccgg gggtggcccg
     1081 ggtctgcctg gccatcgccg agaaccccgc cgacgcgcgc cggctgacca tcaagcgcaa
     1141 caccgtcgcc gtcgtcaccg acggcaccgc cgtgctgggg ctcggtgaca tcggcccact
     1201 cgccgcgctg cccgtcatgg agggcaaggc cgcgctgttc aagcggttcg ccgacatcga
     1261 cgccttcccg atctgcctcg acaccaccga caccgaggag atcatcagca tcgtcaaggc
     1321 catctcgccc gcgttcgccg ggatcaacct cgaggacatc tcggcgccgc gctgcttcga
     1381 gatcgaggcc cggctgcgcg ccgagctcga catcccggtg ttccacgacg accagcacgg
     1441 cacggcgatc gtggcgctgg cggcgctgcg caacgccctg cgtgtcgtcg gcaagcgcct
     1501 cgaggacgtg cgcctggtca tgagcggggc gggcgctgcc gggaccgcga tcctcaagct
     1561 gttcctgcac gccggcgccc aacacgtggt cgtggccgac gtcgacgggg tcgtccaccg
     1621 cggtcgggcc gacgtgctct cgggggagca cccgaaccac gcgtggatcg ccgcgaacac
     1681 caacccggac gacgtcaccg gcaccctctc cgaggccatg cggggcgccg acgtgttcct
     1741 cggtgtctca gcacctgacg tcatcaccga ggccgacgtc gagtcgatgg cgcccgacag
     1801 catcgtgttc gccatggcca acccggtccc cgagatcgac cccgacatcg ccgcgcggca
     1861 cgcggccgtc gtcgcgaccg gccgctcgga cttcgcgaac cagatcaaca acgtgctcgt
     1921 cttccccggc gtgttccgcg ggctcatcga ctccggggtg tcacacgtcg acatggacgt
     1981 gctgctcgcc gccgccgacg ccctggccga cgtcgtgcgc gacgacgagc gcaacgccac
     2041 ctacatcatc ccgagcgtgt tccaccctga cgtcagcacc gtcgtggctg acgcggtggg
     2101 cgccgccgtc gccgcccgcg agggtgcgtg acgccggcgc acaggcgcag ggtcctgctc
     2161 ggactgctcg tcgcggcggt tttcgcccag ttcgtcgtgc tctacgcgcc cgcgcaaccc
     2221 ggcggtctgc cggtgttcgc ccatgccgac aaggtcgtgc acgtgctggt gttcctcgtg
     2281 ccgacggcgc tggctctcgt cgccgggctg cccgcgcggg tggtcgtgcc gctgttcgca
     2341 gcccacgcgg tggtgagcga ggtggtgcag ggggccttgc tgccgatgcg tgccggtgac
     2401 cccttcgacg tgctcgccga cctcaccgga gtcgcgctgg gtgtgctcct gtggaggctg
     2461 cggaggtgac ccggcggcat ccgacgtggt ttttgggatg ctgtggcgct gctaggattg
     2521 tcgttccacc gaggtgctcg gacgcatggc cgcgtccgcc gaggtcgcag agtgcgatcg
     2581 cgccccatcc cacccggtgc cgtcggtgac actcacgtcg aaccggcccc caccgacctg
     2641 acgtccctga cgtctggtcg aggctcgtct tgtttcgccg cgttcgccat cggctcgaag
     2701 acgtgtctgt ctccagtcgt acgaaccatg aaacaggtga atcacccatg cccaagaagg
     2761 caaccgggcc caagaaggcc cgctggacca ctgcccagaa gttcgaggcc aagaaggccc
     2821 cgaagaaggc ccaccaccgc ggccagcccg acggcccgaa gaccgcgccc aagcgtgccg
     2881 cgcgcgccga gtccggcgag tacaccaagc gtccgcgcgc cgagcggtcc ccccgttggg
     2941 accgcgagga cccgcgccgc caggccaccg ccgggcgtcc cgactcccga ggcggcgacc
     3001 cccgaggtgg ctcgcgtggt gacacgcgca gcgactcccg tggcggcgac cgcccctacc
     3061 gccgcgatga ccgcggccac ggccgcgacg accgcggcag ctcgacccgt ggtgacagcc
     3121 gcggccagcg ctactcctcc ggtcagtccg accggggtta cacctcgaac cgcgaagacc
     3181 ggaacgaccg accgaagcgt tgggtctcgg actcgcgcga ggagcgacac gtgcaccgtc
     3241 cccatgatga gcgaccccgc ctcgagcgtg gcgagcgacc ggtgcgtgac cgcgcccccc
     3301 gcgtggccga cccggcccga cgcgatcgcc acgagggtcg gcgcgtcgag cgcgatgccg
     3361 accgtggcgg ctaccagggt cgtgacgacc gtggcggttc ccgcggtggc taccagggtc
     3421 gcgacgaccg tggcggctac cagggtcgtg acgaccgtgg cggttcccgc ggtggctacn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tcgcgtggtg gctaccaggg
     3601 tcgcgatgac cgtggccacc gcggcgcccc ccaggtcgat gacgaccgcc ccctgtcgtt
     3661 cgaggaggcc gaggcccagc gtgccgaggc cgacacctgg acgcactact cgtcgaccgc
     3721 cggtgccgcc aaggaggtca cggccgacaa cggcttcgcc gccctcggtg tccccgccgt
     3781 gctcgtcgag cgcctcgctc gtgacggcat cgccgacccg ttccccatcc aggaggcgac
     3841 gatccccgac gccatcgcag gtcgcgacgt cctcgggcgt ggccgcaccg gctcgggcaa
     3901 gacgctcgcc ttcggtctgt cgatgatcac ccgcctggcc gacggcagcc gcccgcagag
     3961 caagcgcccg cgctcgctga tcctcgtccc gacccgtgag ctggccatgc aggtcagcga
     4021 cgccctcgag ccgttcgtcc acgtgaccgg tctgcgccac aagctcgtcg ccggcggtct
     4081 gtcctacgag ccccagatca aggcgctcgc caagggtctg gacatcctcg tcgcgacccc
     4141 gggccggctc gccgacctca tggagcgtgg cgctgtcgac ctcagcgcgg tggagatcgc
     4201 ggtgctcgac gaggccgacc acatggccga catgggcttc ctgcccgagg tcacggcgat
     4261 cctcgacacc atcccgcagg gcggtcagcg gctgctcttc tccgccacgc tcgacaaggg
     4321 catcgacacc ctcgtcgacc gctacctcgt cgacccggtc acccactcga ccgacgacgc
     4381 gcaggcgtcc gtgacgacga tgagccacca cgtgctcctc atcgaccccc aggacaagaa
     4441 ggccatgacg gccgagctcg ccggtcgcgg tgggcgggtc atcgtcttcg ctcgcaccca
     4501 gctcggttcc gaccgcatcg ccggcgagct gcgcgagcgg ggcgtgcttg cggcatccct
     4561 gcacggtggt ctgtcgcagg ccgtgcgcaa ccgcgtgctg ggcgccttcc gtgacggctc
     4621 gatgaacgtc ctcgtcgcga ccgatgtcgc cgcgcgcggc atccacgtcg acgacgtcgg
     4681 catggtcgtc caggtcgacc cgccgaccga ccacaaggac tacctgcatc gtgccgggcg
     4741 caccgcccgg gctggggagg agggcgcggt cgtcatgctc gccctgcctc accagcgcaa
     4801 gatggtcacc cggatcatcg agggtgccgg cgccgaggcc aagaccgcca aggtcgcccc
     4861 cggtgacgcg acggtccagg ccatgggtgg tcgcgcaccg agcggtgtgc cggtgccgga
     4921 tgccgtgtgg cgaccgatcc tcgaaggcgg caagaacggt ggtcgccgtg gcggtggtcg
     4981 tccgggtgga cgccccgcag gtcgccctgg tggccgtccc ggtggtcgcc cctcgcactc
     5041 cggtggcggg cactcccgtc acgggtccgg acgtccggcc ggtggccggg gcagaatgga
     5101 cgggtgagca cgagcgacac cacgggcaag ctcctcgtcg caaccccgca gatcgaggag
     5161 ggcgtgttcg cacgctccgt cgtcctcgtg ctccatcacg atgacgacgg cgcccagggg
     5221 ttggtgctca accggcccgt cgacgccgac atcgacgcgg tgctgcccgg gtgggaggcc
     5281 cacgccagtt ctccggccaa ggtcttccag ggcgggcccg tgcagctcga ctcggcgctc
     5341 ggcctggccg tcgtccccgg ggacgcgccc gctccctcgg gcgttcgccg gttgttcggc
     5401 tcggtggcgt tggtcgacct cgacgcccag ccatcgctcg tcgtgccggc ggtcgctggt
     5461 ctgcggatct tcgccgggta tgccgggtgg tctccggggc agctcgagga cgagatcagg
     5521 accggttcgt ggttcgtcgt cgacagcgag agcggcgacg tcttcaccga cgagccggcc
     5581 ggtctctggc agcgcgtcct gcgacgtcag ggcgatccgt tgcgctgggt cgcctggtac
     5641 ccgcgcgacc cgtcgctgaa ctgaccccgc tactcgatgg cgtacggatt caggatctgc
     5701 cggcccatga gccgggaggc ctcctccgtc gtcaagcgcg cctgatccgc acagtcgcgg
     5761 taggaatttc ggatgccgtt cacgacctcg tcgaggatgt tgcgggccgc gcgctggctg
     5821 aggaggtagg tgggtgctga ctccagcagc agggtcagtc tcgccgcacg ttgcccgttg
     5881 gggccgaagg ccattgcctg attcgcttca cggcccgatc gtggctgcgg gcagaggtcg
     5941 taggccggcg tcagcgccaa ggtgtggcca tcccagaacg ccgcgtgatt gcgggcgtgg
     6001 tcatcggtgt tgccgatgca gatgttgacc gcgatccggc ggaacagttc cgccagcgtg
     6061 ccggcggggt cgctgaaccg cgccctcacg acatcggcga cgtcggtgta ggtcgcgtat
     6121 cgagccatca tctcgtccag gccaaggatg gtgagtgccg acaccatctg ccgtcgttcg
     6181 tcggccatcg tcgtgcgatc gaagcgctca acgagcagca cgtccctgcc gagggaccgg
     6241 gtgacgtcga cgttcggaac tcgaacgccg gcggccctgg ccaagagcat cccgagggcc
     6301 tccgccttca ccacggggta cgtgtcgctc gcggacgaga acttcgcgac gaggtgtcgt
     6361 ttgccgtcgc tgatcagcgc cttgggtcgg gccccaccga tcgacgttcc ccggaccaag
     6421 gcctcgccga gctcctccgg gagtgcctcg ccggcgtcca gcgcttgggc agcagacacc
     6481 agctggtcga gcgttgcttc acctccgcgg tggacgtaga cgtcggggcg cgactggaag
     6541 tccaaggcgc cgatgcggtt tgatcccgac tcaagcaggt acgtcaacgg cccgaggtcg
     6601 ccgttgtcgg agtgccgcgt cagatgtccc gtgcgacgcg ccaggatcac ccgctgaccc
     6661 caggagtcgg gccccgcatc acggatggcg gagggcagac tcaggccggg cagtggctcg
     6721 atgcggccgg ggcggagggg cagctctggg aggtagagcg caatggcgtc gctgcggttg
     6781 aggtaggaac gcccgtaggt gaaggtgacg atgccgttgc tctgggcctc gagcagccct
     6841 gccacgactg gatccgtcgc gcccggaagc caaatccaga cgtatgcctc gcgcggttca
     6901 gaagtcgtca cggaccggct cctcgcgtgg tcggactctc gtggggagca acgccaacgt
     6961 ctcgcgcccg tgacgcacct cggccacgag ggaaggttcg tcgcgcccga acagggtgac
     7021 gccgcacagg actgcggcct ccaacgccgt gcccagtgcc acggttgggg cgcccctctc
     7081 gatcttggtg atcgtcacgg ggtgtacccc gacccgctcg gccaactcgg cgaccgtcca
     7141 ccctctttcg cggcgtccct tggcgacctg ctggccgagc agctccgctg ccagcacggt
     7201 cggggcgctg tagggcaggg cgtgctttgc caccgtagcc tccttaaaaa cactcttaga
     7261 gcgctaagag tacctttgaa tgtttcgtat cgccacgggt ggggcgggta ggggcagtga
     7321 catggcccgg cgggcagccg cctcagctcc cgactccgga cgggtgggcg catacactcg
     7381 gcgccatgag cgagacgatg ccgccccttg acgacccgaa cgctccggcc gggcagccag
     7441 cgacccgcac cgccgtgctc gagcgcaccg aggccgtgcc ccaggtcgcc gaacccggtg
     7501 accacgagcg cttctcgcac tacgtgcgca aggagaagat cctcgagagc gccctgtccg
     7561 gcgagccggt caccgccctg tgcggcaagg tctgggtgcc cgggcgcgac ccgaagaagt
     7621 tcccggtctg cccgacctgc caggagatct acgacggtct gcgcgccccc caggacggcg
     7681 acaagtaggc ggcatccgcc ctctccctcc ctgcgagaag ccgcctcgac caccagaaca
     7741 cggcgtgtcg cccggcctgt cgcagggaat gtcggcaagg gcggggtggt gccagcggtg
     7801 aggctgtcga gcggttaggg ttgccgtcgc ccatgagtac ctctgccgcc ggacacctgt
     7861 cacccgcctt ccccgagcgg gcggcgtggg gaaccgcttc gaagctgcgc gcctggcagg
     7921 aagaggcgct ggccagctac ctgcgcagcg agagccgcga cttcctcgcc gtcgccaccc
     7981 ccggcgccgg caagaccacg tacgcgctgc gggtcgcgac cgaactgctc gaccgcggca
     8041 tcatccgggc ggtcaccgtc gtcgccccca ccgagcacct gaagacccag tgggcggatg
     8101 ccgcggcgcg cgtgggcgtc cacctcaacc ccgcgttctc caactccagc ggggtgcagt
     8161 cccacgagta ccacggtgtc gcgctcacct atgcgcaggt cgccgccaac ccggcgttgc
     8221 accgccgccg cacgatcgac accccgaccc tggtgatcct cgacgagatc caccacggcg
     8281 gggacgcgaa gtcgtggggc gacggcatcc gtgaggcctt cgagccagcc acccgccgcc
     8341 tgtcgctgac cggcacgccg tttcgttccg acacgagccc catcccgttc gtgcgctacg
     8401 agatgggtga ggacggcatc ctgcgctcgg cctcggacca cacctacggc tacgcggagg
     8461 cgttgcgcga cggggtcgtg cggcccgtgc tcttcctcgc ctacggcggc gcgatgcggt
     8521 ggcgcaccaa ggccggcgac gaggtctcgg cacgcctggg tgagcccctc accaaggacc
     8581 tcaccgcgca ggcctggcgc accgcgctcg accccaaggg ggagtgggtg ccctcggtgc
     8641 tcgctgccgc agacaagcgc ctgagcgagg tgcgccgcca cgtgccggat gccggtgggc
     8701 tggtgatcgc gtcgaaccag acggccgctc gcgcgtacgc caagctgctc cagggcatca
     8761 ccggcgtggc gcccaccgtg gtgctctccg acgatgccgg ggcgagcacc cgcatcgaga
     8821 agttcgcggc cgacacctca cggtggatgg tggccgtgcg gatggtgtcc gaaggtgtcg
     8881 acgtgccgcg cctgtgcgtg ggcgtctatg ccacgtcgac ctcgacgccg ctgtacttcg
     8941 cgcaggccgt cggacgcttc gtgcgggccc gcaagcgggg tgagacggcc tcggtgttcc
     9001 tgccctcggt gccggtgatc ctcagccacg cagccgcgat ggaggtcgag cgcgaccacg
     9061 ccctcgatcg caagaccacc gccgagggtg aggcgtcgct ctgggcggag gaggagggcc
     9121 tgctcgccgc ggcgaaccgc accgagaccg aggccgagct cgaggacagc tccttcgagg
     9181 cgctggagtc cgacgcgcac ttcgaccacg tgctcttcga cgcccagcag ttcggcctgc
     9241 acgcggccgt gggctcgcag gaggagcagg actacctcgg gctcccgggc ctgctggagc
     9301 ccgaacaggt gacgaccctg ctcaacgagc ggcaggcacg ccagtccaag tcggccaccc
     9361 gccgaggtgg ggcgccagcg ggaggtgcag cgacggcatc cggcccgtcg gtggtctctg
     9421 cgcaccgcgc gctggcctct cagcgcaagg agctgaacaa gctcgtggcg gcgtactcgt
     9481 cgaagacggg ggcggcgcac gcggccatcc acatggacct gcgccgcacg tgtggcggcc
     9541 ccgagctcgc gtcggcgacg agtgagcagg tcaccgagcg gatcgaccgg atcaggcgct
     9601 ggttcgtcgg ccgccgctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     9721 tgcctcgacg agggggccgg tgcggtggtc caggtgggtc accaggcaga tcgccgtgtt
     9781 cgcgcgcagg acgtgcgtgt cgtcgatgat ctcgtcgatg acccggccga ggtcggcgtt
     9841 gtcgcgggcg acgatgcgca cgatgaggtc gccgacgccg gtgatcgagt ggatctcgag
     9901 cacctcgggg atcgcggaca ggtgcgccac gacgggggca tgaccctgcc gctgccggat
     9961 ctcgagcgtg cagaacgctg tcacgggata gcccagggcc tcggaactga cgtggggcgc
    10021 gtaggaggtg atgactccgc gttcctcgag ccggtcgagc cgagcctgca ccgtgccccg
    10081 cgcgacgccc agcgtcctgg aggcgccgag cacgccgaca tggggttgct cggtgaacag
    10141 ggcgatgagg cgggcgtcga gatcgtcgat ccctgtctgt cggggcatgg tcacattgtt
    10201 cgggttgacc ccctgtcagg gcaacaccat gcgcaccctg accagccagg aggcgcaccg
    10261 ttgcacaccg ctgcccacag gccccacagt gatcggatga ctgacacgac catgacgcct
    10321 gccagccaca tcgacctcac cgagcaggag cgccaggccg gcctggacct gcagcagctc
    10381 aagcagctcg tcggcctcgt cgactacgac gagcagcagg accccttccc ggtgaccggg
    10441 tgggacgcgc tcgtcttcgt cgtcggcaat gccacccagg cagcgcacta ctaccagagc
    10501 gcctggggca tggagctcgt cgcctacgcc ggccccgagc acggtcagcg cgaccacaag
    10561 gcgttcgtcc tgaagtcggg gtcgatccgc ttcgtgctca agggtgcggt cagcccggac
    10621 agcccgctca tcgcccacca cgccgcccac ggtgacgggg tcgtcgacat ctccctcgag
    10681 gtgcccgacg tcgaccgctg catcgagcag gcccgccgcg ccggcgccac catcctccag
    10741 gagcccgaga cggtcagcga cgagcacggc agcgtgcgca tcgcggcgat cgcgacctac
    10801 ggcgagaccc gccacaccct cgtccagcgc gccgtcgacg gcgtgacgta tgccggcccg
    10861 tacctccccg ggtacgtcgc cgccgcctcc acgtacgtca agcgcgatgg tgcccccaag
    10921 cggctgttcc aggcgctcga ccacgtcgtc ggcaacgtcg agctcggcaa gatggacgag
    10981 tgggtcgggt tctacaacaa ggtcatgggc ttcgtgaaca tggccgagtt catcggtgac
    11041 gacatcgcca ccgactactc cgcgctgatg tccaaggtcg tcgccaacgg caaccaccgg
    11101 gtgaagttcc ccctcaacga gcccgcgatc gcggcccgca agagccagat cgacgagtac
    11161 ctcgagttct acgccggccc cggcgcccag cacctggcgc tggcgacgaa cgacatcgtg
    11221 cgcaccgtcg acgagctgcg caaggagggt gtggagttcc tcgacacccc cgacgcgtac
    11281 tacgacgacc ccgagctgcg cgcccgcatc ggtgaggtgc gcgtgccgat cgaggacctc
    11341 aagtcgcgcg gcatcctcgt ggaccgtgac gaggacggct acctgctcca gatcttcacc
    11401 cgccccctcg gcgaccgccc gaccgtgttc ttcgagatca tcgagcggca cggctcgctc
    11461 ggcttcggca agggcaactt caaggccct
//
LOCUS       DAOSOC010000075        11204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5791_length_11204_cov_10.004754, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000075 DAOSOC010000000
VERSION     DAOSOC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5791_length_11204_cov_10.004754"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PL014_07200"
     CDS             <1..245
                     /locus_tag="PL014_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT56912.1"
                     /translation="DGGAARRFQNEVEVGMVGVNVPIPVPMAYYSFGGWKNSLFGDSH
                     AHGMEGVNFFTRGKVVTSRWLDPSHGGIELGFPQNT"
     gene            complement(307..1056)
                     /locus_tag="PL014_07205"
     CDS             complement(307..1056)
                     /locus_tag="PL014_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT56913.1"
                     /translation="MELASSTGALALMALAAAIIGYSKTALGGLGVVAVAIFATVLPA
                     RESTAAILMILIVGDVIACWHYRRDPDWGLIRRLLPAVIPGLLLGTLFLRVVSDDTLR
                     RWIGAVLLVLLGLQLWVKWRARDTPSTTHRHPGAAIAAGSAMGFTTMTANAAGPVMTL
                     YLSAAGIDKRRFVGTSAWFFLIVNLTKVPFSIGLGLLHPVDAVRALALAPFVLLGGML
                     GYATVRRISQRTFDVAVLLASALAAAALLLT"
     gene            complement(1062..1469)
                     /locus_tag="PL014_07210"
     CDS             complement(1062..1469)
                     /locus_tag="PL014_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT56914.1"
                     /translation="MADHFLDATIILLAAGEPSTAQHACRSFLEAQAASGSTLHMSVE
                     GVQEVLFHRMRMVDRGTAVAEARDVQEMVVLHRFDEGVLQTAISLVATSTVRGRDAVH
                     AATAIRAGFDAIVTTDSDFDGIPGLRRIDPRDT"
     gene            complement(1462..1752)
                     /locus_tag="PL014_07215"
     CDS             complement(1462..1752)
                     /locus_tag="PL014_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOT56915.1"
                     /translation="MSTLERRLQLLLTQEQHARLAEVAKESGRSVNAVIREAIDRQHP
                     DEATKRAAALKRWLDRTATPVEAGLGPTPSAAQLKAEYEQDEIDHMDAILRG"
     gene            complement(1854..3173)
                     /locus_tag="PL014_07220"
     CDS             complement(1854..3173)
                     /locus_tag="PL014_07220"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HOT56916.1"
                     /translation="MTLAPERTAPQTIPAHASTKPNGINPRLSVTSLTPRPQRPALKK
                     SGRPNPAAHLTPEQIEGIGVELDALRKQVMDSRGERDAAYIRKVIKTQRYLEMGSRAV
                     LLFSGVKVKGFRPAWWIGTAGLSVAKILENMEIGHNVMHGQWDWMRDPKIHSTAWEWD
                     NASPSSQWKHSHNEIHHTFTNVLGRDNDLGYGILRVDEDQRWSPFFLFQPIWHVGNAL
                     LFEYSIAAYDLELGGYLAKRKRMSEEEKAKFRADAAEVVAKIKRQATKDYVVHPALAA
                     ATFTGSARHTLTANVVANLVRNVWTNTVIICGHFPPGIATFEKTSIEGETRGEWYLRQ
                     MLGSGNISGSKAMHVMSGNLSHQIEHHLFPDMPSNRYAEIAPQIRDLMERHDLPYVTG
                     SLPKQAASVYWKVVELSLPNKVEGRRRRDIVRLSLKKAWKKKSLGRF"
     gene            complement(3210..4370)
                     /locus_tag="PL014_07225"
     CDS             complement(3210..4370)
                     /locus_tag="PL014_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HOT56917.1"
                     /translation="MSAPTVDRPSREAGMPRARLGAHAPGPIGGVVRSARRVVEALAT
                     PLVPGDYLDLVDPLRAGADLRGRIIDVRPETSDAVTIVIRPGRGWLGHVPGQYIRIGI
                     DIDGVRQWRAYSLTHRADGSPAAGIPAGCIAITTKAIPDGVVSNHLVRRARPGTLVML
                     DQATGDFCLPTPAPEKVLFLTAGSGITPVMGMLRNHLGELADAAHVHCAPTASDVIFA
                     DELHGFADSGRLALTLNLDDEHGVLDLARLDTLVPDWRERETWLCGPTGLLDAAEAHW
                     AAAGLADALHTERFRPSTIEPGEGGTVTFSASGRTVEADGGTPILDAGEAAGVLMPSG
                     CRMGICFGCVVTMREGAIRDLRTGDITVVEPGDELPVQTCVNAVAGACDLDL"
     gene            4527..4913
                     /locus_tag="PL014_07230"
                     /pseudo
     CDS             4527..4913
                     /locus_tag="PL014_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            5103..5786
                     /locus_tag="PL014_07235"
     CDS             5103..5786
                     /locus_tag="PL014_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT56918.1"
                     /translation="MDQILLKDLPEQGLAVRRPETLRRWLAAYAAASSTTAAYSRILD
                     SATAGDGSHPAKTTTIAYRDHLTQLFLLDPVPGWSPSRSPLRRLQQAPKHQLADPGLA
                     AAILGLTAQRLLSPHGAHVAGPLFEALATLSVRVAAQSAEARVGHLRTGNGDREVDLI
                     VEGESGQVLGIEVKMSASVGDADVRHLRWLREQLGSDVVDLVVLTTGTHAYRRPDGIA
                     VIPLALLGA"
     gene            complement(5884..7524)
                     /locus_tag="PL014_07240"
     CDS             complement(5884..7524)
                     /locus_tag="PL014_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT56919.1"
                     /translation="MSTPENTPTPVPADTAPEHLEAIVTSDAVLELPPEKSGFGEGNV
                     GEVLLDLAGLTKSFGSVRANRDITMHVRRGEIVALLGENGAGKSTLVNQIFGLITPDS
                     GTVTVKGDSTPIKDPRDAIARGIGMVHQHFQLVPVMTVAENMILGHEPTRGGAMLDLE
                     SAREMVRTLSAKHGLPVDPDAEVEDLPVGTQQRVEILKALSRSVDLLILDEPTAVLTP
                     QETDELLGVMKELAASGTSIVFITHKLREVLAVADRVYVLRQGAVVGEVATKDTDARG
                     LATMMVGREVVLSIDKADAAPTDTVLTITDLHVLDDRELGAVNGFTLSVRAGEIVGVA
                     GVEGNGQRELVEALAGMRKVVSGQMTLGDLDLTKANPHQTHHAGVGHIPEDREKHGLV
                     ASYSIADNLVLNEFDQAPYSKSGVRQFDAIKEHAEAVRTEFDIRSSGVHQSSGSLSGG
                     NKQKVVVAREMSFKPRLLMAAQPTRGVDVGSIEFIHRRIVDARDAGAAVLLVSAELDE
                     ILSLSDRIAVVHGGKVVAELPARGADRTEIGRLMAGDH"
     gene            complement(7524..8522)
                     /locus_tag="PL014_07245"
     CDS             complement(7524..8522)
                     /locus_tag="PL014_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56920.1"
                     /translation="MGAWIAKNRTTVIVSVAVIVAAYAFYYLKNDIAPSMYVSAWGYA
                     IPLVLAALVGVIGERSGVVNIGIEGQMLVAAFAGFFAAAYSGSLLVGVLAGIGSGLIL
                     GGFLAWTTVKWRMDQIIAGVVLNIIATGITSFYYQPGQLLPGLMPKFDIWPLSKIPLI
                     GEVFFSGTSIFATLAILAAIGVHFALFHTRWGLRTRAVGEYPSAADTAGINVERLRLI
                     NVTLAGTLAGCAGVYLSMDASSSFERGMVAGRGFLALAIMIMGAWRPLRALAMAIFFG
                     FINAVASQLQAGGSIDIPPQLTGTLPFVVTIVVLAIAAGRVRAPGAVGQPYVKGDQ"
     gene            complement(8524..9678)
                     /locus_tag="PL014_07250"
     CDS             complement(8524..9678)
                     /locus_tag="PL014_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT56921.1"
                     /translation="MSETPTVADKAAAPPPSSGGTSGDKKPAQPLAYLLTHQSGVVIL
                     VALVIALLVGAALIRYQGVSPWYAYETIIREALLVDGGLTRTLQKTAPLVLTGLAVVI
                     PLRVGLFNIGGQGQLIFGAMFGAWVAYAAGGLGFLGAVCGMVAGMVAGAFIGWIAGVL
                     KATRGVHEVITTIMLNYIAAGVTWWLVTGPLQAEGSKTSGIPQTELIPDAARVGAVAG
                     IPIGFVIALALAIACWWMLSRTTLGFKLNTVGANKHAARYAGININRIIALSMVFGGG
                     LAGLAGALEAAGTLHRFEPAIGGTLGFDGITIALLARANPLATIPAALLVGILRTGAP
                     GLQFATGIAPEVVDLLLAIILLMVSIPVLGKWIFRSRADKVTAASTSWGN"
     gene            complement(9852..10940)
                     /locus_tag="PL014_07255"
     CDS             complement(9852..10940)
                     /locus_tag="PL014_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT56922.1"
                     /translation="MKSTRYLVAVPAALALTLAGCGGTSGNTESPAASGSAAPGAPTD
                     DSNCADEAVFCIGLVTDTGKVDDKSFNQSAHEGALAAAEQTKGFYKYIETQDAKDYAA
                     NMAQFTNKKYDAIVTVGFLMTDATLEAAKANPGTRFIGVDQGYDPAAVSGKNVTGLIF
                     PEDQAGYGAGYLAGLMTKTNKLGQVLGLEIPPVQKFAKGFEAGAKAANPAITVTTVYH
                     PAGDNAFNDPVWGAGEAKKQLAQGADIIFGAGGNTGNGALQEVAKAKGAGTEVFCIGV
                     DTDQWDTVPAAQPCLITSAMKLITEGVTETLVTAKDGDFAENQTLGKAGLAPFHDFES
                     VIPADVKAKVDEVVKQMEAGTLQTGVTL"
     gene            complement(11085..>11204)
                     /locus_tag="PL014_07260"
     CDS             complement(11085..>11204)
                     /locus_tag="PL014_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT56923.1"
                     /translation="PVTASEVAATVSFLASPAASGITGTTIAVDAGWSAGPGW"
BASE COUNT         1717 a         3600 c         4122 g         1765 t
ORIGIN      
        1 acgacggtgg cgccgcgcgg cgcttccaga acgaggtgga ggtcggcatg gtcggggtca
       61 acgtgccgat ccccgtgccc atggcgtact actccttcgg tgggtggaag aactccctct
      121 tcggcgacag ccacgcgcac ggcatggagg gggtcaactt cttcacccgc ggcaaggtcg
      181 tgacctcgcg ctggctcgac ccgagccacg gtggcatcga gctcggcttc ccgcagaaca
      241 cctgatcccg acttcttgca cggcagccac ccggatcagg gtggctgccg tgcaagaagt
      301 gcctgctcag gtgagcagca gggctgctgc cgccagggcg gaggcgagca gcacggcgac
      361 gtcgaaggtg cgctggctga tccgcctgac cgtcgcgtag ccgagcatcc cgccgaggag
      421 gacgaacggt gcgagcgcga gggcgcgcac ggcatccacg gggtgcaaca gcccgagccc
      481 gatcgagaac ggcaccttcg tgaggttgac gatgaggaag aaccacgcgc tcgtgccgac
      541 gaagcggcgc ttgtcgatgc ccgcggccga caggtacagg gtcatcaccg ggccggcggc
      601 gttcgcggtc atcgtcgtga agcccatcgc cgagcccgcg gcgatcgccg ctcccggatg
      661 ccggtgggtc gtcgagggtg tgtcgcgggc gcgccacttc acccacagct gcaggccgag
      721 gagcacgagc aggacggctc cgatccagcg gcgcagggtg tcgtcgctga ccacccgcag
      781 gaagagggtg ccgagcagca ggccggggat gacggcgggc agcaggcggc ggatgaggcc
      841 ccagtcgggg tcgcggcggt agtgccagca ggcgatgacg tcgccgacga tcaggatcat
      901 gaggatcgcc gctgtcgact cccgggccgg cagcacggtc gcgaagatgg ccaccgcgac
      961 gacgccgagc ccaccgaggg cggtcttcga gtacccgatg atcgccgcgg cgagagccat
     1021 gagggccagg gcgcccgtgc tggaggccag ttccacgccg gtcaggtgtc gcgggggtcg
     1081 atgcggcgaa ggcccgggat gccgtcgaag tccgagtcgg tggtcacgat ggcgtcgaac
     1141 cccgcgcgga tggcggtagc ggcgtggacg gcatctcggc cacgaacggt cgaggtggcc
     1201 acgagggaga tcgccgtctg caggacgcct tcgtcgaacc gatgcaggac gaccatctcc
     1261 tgaacgtcgc gggcctccgc gaccgcggtc ccacgatcca ccatgcgcat gcgatggaag
     1321 aggacctcct gaaccccctc gacactcatg tgcaacgtcg agcccgaggc ggcctgggcc
     1381 tcgaggaagg accggcacgc gtgctgtgcc gtgctgggtt cgccagcggc gagcaggatg
     1441 atcgtggcgt caaggaagtg gtcagccacg caagatcgca tccatgtgat cgatctcgtc
     1501 ctgctcgtac tcggccttca gctgtgctgc gctcggtgtt ggccccagac cggcctccac
     1561 cggcgtcgcc gtgcggtcga gccatcgctt cagggcagcg gcccgcttgg tcgcctcatc
     1621 ggggtgctga cggtcgatgg cctcacggat caccgcgttg acgcttcggc cgctttcctt
     1681 ggccacctcc gccaggcggg catgctgctc ctgcgtgaga agcagctgaa gtcggcgttc
     1741 cagggtggac atgcacacac catacacatg tgcaacgaca ggcaaacgga tgccgtggcg
     1801 agctgacaat gtcggctcgc cacggcatcc gagatggtgg tgaaacccgg gcctcagaag
     1861 cggccgagcg acttcttctt ccaggccttc ttcagcgaca ggcgcacgat gtcgcggcgg
     1921 cgacgcccct cgaccttgtt cggcagcgag agctcgacga ccttccagta caccgatgcc
     1981 gcctgcttgg gcagcgagcc ggtgacgtac ggcaggtcgt gacgctccat gaggtcgcgg
     2041 atctgcgggg cgatctcggc gtagcggttg ctcggcatgt ccgggaagag gtggtgctcg
     2101 atctggtggc tgaggttgcc actcatcacg tgcatggcct tggagccgct gatgttgccc
     2161 gagccgagca tctgccgcag gtaccactcg ccgcgggtct cgccctcgat ggaggtcttc
     2221 tcgaaggtcg cgatgccggg cgggaagtgg ccgcagatga tgacggtgtt ggtccacacg
     2281 ttgcgcacga ggttggcgac gacgttggcc gtcagggtgt gccgggccga accggtgaac
     2341 gtcgcggcgg ccagggccgg gtgcacgacg tagtccttgg tggcctggcg cttgatcttc
     2401 gcgacgacct cggcggcgtc ggcacggaac ttcgccttct cctcctcgct catccgcttg
     2461 cgcttcgcga ggtagccacc gagctcgagg tcgtacgccg cgatcgagta ctcgaagagc
     2521 agcgcgttgc cgacgtgcca gatgggctgg aagaggaaga acgggctcca ccgctggtcc
     2581 tcgtccacgc gcaggatgcc gtagccgagg tcgttgtcgc ggccgagcac gttggtgaag
     2641 gtgtggtgga tctcgttgtg gctgtgcttc cactggctgc tcggggaggc gttgtcccac
     2701 tcccacgcgg tcgagtggat cttcgggtca cgcatccagt cccactggcc gtgcatgacg
     2761 ttgtggccga tctccatgtt ctcgaggatc ttggccaccg acaggcccgc ggtgccgatc
     2821 caccaggccg ggcggaagcc cttgaccttc acgccggaga acagcagcac ggcgcggcta
     2881 cccatctcga ggtagcgctg ggtcttgatg accttgcgga tgtaggcggc gtcgcgctcg
     2941 ccacggctgt ccatgacctg cttgcgcagg gcgtccagct cgacgccgat gccctcgatc
     3001 tgctcggggg tgaggtgtgc ggccgggttc gggcggcccg acttcttcag cgcggggcgc
     3061 tgggggcgcg gggtcaggct ggtcaccgac agtcgcgggt tgatgccgtt cggcttcgtc
     3121 gacgcgtggg cggggatggt ctgcggcgcg gtgcgctcgg gggcgagagt catgtggcgg
     3181 tgtccttccg ggtggtcagg aggtcagggt cagaggtcga ggtcgcaggc gccggcgacg
     3241 gcgttcacgc aggtctgcac gggcagttcg tcgccgggtt cgacgacggt gatgtcaccg
     3301 gtgcgcaggt cgcggatcgc gccctcgcgc atggtgacga cacagccgaa gcagatgccc
     3361 atgcggcatc cgctcggcat gaggacgccg gctgcctcgc cggcgtcgag gatcggggtg
     3421 ccgccgtcgg cctcgacggt gcggccgctg gcggagaagg tgacggtgcc gccctcgccg
     3481 ggttcgatgg tgctcgggcg gaaccgctcg gtgtgcagcg cgtcggcgag cccggcggcc
     3541 gcccagtgcg cttcggcggc gtcgagcagg ccggtcggcc cgcacagcca cgtctcacgc
     3601 tcgcgccagt cgggcacgag ggtgtccagg cgcgcgaggt cgagcacgcc gtgctcgtcg
     3661 tcgaggttca gggtcagggc gaggcggccg ctgtcggcaa agccgtgcag ctcgtcggcg
     3721 aagatcacgt cggatgccgt gggcgcgcag tgcacgtggg cggcgtcggc gagctcaccc
     3781 aggtggttgc gcagcatccc catcaccggg gtgatgccgc tgccggcggt gaggaagagc
     3841 actttctcgg gcgccggggt cggcaggcag aagtcgccgg tggcctggtc gagcatgacc
     3901 agggtgccgg ggcgggcccg gcgcacgagg tggttggaga cgacgccgtc ggggatcgcc
     3961 ttggtcgtga tggcgatgca ccccgccggg atgccggccg cggggctgcc gtcggcacgg
     4021 tgggtcaggc tgtaggcgcg ccactggcgc accccgtcga tgtcgatgcc gatccggatg
     4081 tactgcccgg gaacgtgccc gagccagccg cgaccggggc ggatgacgat cgtcacggca
     4141 tccgaggtct cggggcggac gtcgatgatc cggccgcgca ggtcggcccc ggcgcgcagc
     4201 gggtcgacga ggtcgaggta gtcgccgggc acgagcgggg tggccagggc ctcgacgacg
     4261 cggcgcgcgc tgcggaccac gccgccgatc gggccgggag cgtgggcacc gaggcgggca
     4321 cgcggcatcc cggcctcacg ggagggtcgg tcgacggtgg gggcactcat tacgccagcg
     4381 taacctacgg aaacgtaact tacgaatcgg taaccaccgc gggtgcgaac cgtgcctgct
     4441 gaaggtagtg cctgatgacg gttaaaggta gtgtagcggt gctgctagag gtagtgtaga
     4501 cagtgttgag cagcagagga gcagatatgt cgccagaggt ctactctcgt cgatcactgg
     4561 atgacgaact cgacgaactc ctgccccttg cctcggccat cgcgctcgag ggacccaagg
     4621 gtgtcgggaa gacacgaacg gcgctgcggc gatcggatgc cgcctggttc cttgacgacg
     4681 aatctcaccg cgctctggtg tctgccgacc ccacgttcgc cgccgcccca ccggggaccc
     4741 tgctcatcga tgagtggcag cgactgccgt ccgtctggga ctcggtgcgt cgacgcgtcg
     4801 acgagggggc accggcaggc cgattcctgc tgtcggggag cgcgactccc gtcggcggca
     4861 ccgtcgttca cagcggggcg ggccgcatcc tgtcgctccg gatgaggccg atggcgctgt
     4921 acgagcgccc cggcgcctca ccgagcatcc gactgcgaca tctcctggat ggccgccaag
     4981 gcccgatcgg cggaacgacg agcaccagcc tcgtggacta cttcgaggcc atcgagtcca
     5041 cgggtctgcc tggcctcatg cgcgaacccc cacggctgcg cagaccactg ctgcgcagct
     5101 acgtggacca gatcctgctc aaggatctgc cggagcaagg gctggccgtg cgacggccag
     5161 agaccctgcg acggtggctt gccgcgtacg cggccgcctc gtcgacgacc gccgcctact
     5221 cgcggatcct cgacagcgca acggcgggcg acggcagcca cccggcgaag acgacgacga
     5281 tcgcctaccg cgatcacctc acgcagctct tcctgctcga tcctgtaccg gggtggtcac
     5341 cgagccgcag tccgctgcga cgcctccagc aggcgccgaa acaccagctg gcggatcctg
     5401 gtctcgcggc cgccatcctg ggactcaccg cgcagcgctt gctcagccca cacggggcgc
     5461 atgtggccgg accgctgttc gaggcactgg cgaccttgtc cgtcagggtg gctgctcaga
     5521 gcgccgaagc cagggtgggt catctccgta cgggcaacgg cgaccgagag gtggacctca
     5581 tcgtggaggg ggagtcgggt caggtgctgg gaatcgaggt caagatgtcg gccagcgtcg
     5641 gcgatgccga tgtcaggcac ctcaggtggc tacgtgagca actcggcagc gacgtcgtcg
     5701 acctcgtcgt cctcacgacg ggcacgcacg cctaccggcg acccgacggt atcgcggtga
     5761 ttccgctggc gctcctgggg gcgtgactct ccgccggtcc cgactcttcc tacgagaagc
     5821 cgcccgaccc gccgtttcgt ggcgtgtcgg gcggcttctc gcaggaagag tgcggaaggt
     5881 cagtcagtgg tctccggcca tcaggcgacc gatctcggtg cggtcagccc cgcgcgcggg
     5941 cagttcggcc acgaccttgc cgccgtgcac gaccgcgatc cggtcggaga gcgacaggat
     6001 ctcgtcgagc tcggccgaga cgagcagcac cgcggcgccg gcgtcacggg cgtcgacgat
     6061 gcgccggtgg atgaactcga tcgatccgac gtcgaccccg cgcgtgggct gggccgccat
     6121 gagcaggcgc ggcttgaacg acatctcgcg ggctacgacg accttctgct tgttgccacc
     6181 ggagagcgac ccggaactct ggtgcacccc ggaggatcgg atgtcgaact cggtgcgcac
     6241 cgcctccgcg tgctccttga tggcgtcgaa ctggcgcact ccgctcttgg agtacggcgc
     6301 ctggtcgaac tcgttgagca cgaggttgtc ggcgatggag tacgaggcga cgagtccgtg
     6361 cttctcgcgg tcctcgggga tgtgcccgac cccggcgtgg tgggtctggt gcgggttggc
     6421 cttcgtgagg tcgaggtcac cgagggtcat ctgccccgac acgaccttgc gcatcccggc
     6481 cagggcctcg acgagctcgc gctggccgtt gccctcgacg ccggccacac cgacgatctc
     6541 accggcgcgc accgacagcg tgaacccgtt gacggcaccg agttcacggt cgtcgagcac
     6601 gtgcaggtcg gtgatcgtga gaacggtgtc ggtcggtgcg gcgtcggcct tgtcgatcga
     6661 cagcacgacc tcgcggccga ccatcatcgt cgcgagccca cgggcgtcgg tgtccttggt
     6721 ggcgacctcg ccgacgaccg cgccctggcg cagcacgtag acgcggtcgg ccaccgcgag
     6781 cacctcgcgc agcttgtgcg tgatgaacac gatggacgtg ccggaggctg cgagctcctt
     6841 catgacgccg agcagctcgt cggtctcctg cggcgtgagc accgcggtcg gctcgtcgag
     6901 gatcagcagg tcgacgctgc gcgagagcgc cttgaggatc tcgacacgct gctgggtgcc
     6961 gacggggagg tcctcgacct cggcgtcggg gtcgaccggc aggccgtgct tggccgacag
     7021 ggtccgcacc atctcgcggg cgctctcgag gtcgagcatc gctccgcctc gcgtcggctc
     7081 gtgtccgagg atcatgttct cggccaccgt catgacgggc acgagctgga agtgctggtg
     7141 caccatgccg atgccgcggg cgatggcgtc gcgcgggtcc ttgatggggg tggagtcgcc
     7201 cttgaccgtc accgtccccg agtccggggt gatgaggccg aagatctggt tgaccagggt
     7261 ggacttgccg gccccgttct caccgagcag tgcgacgatc tcgccgcgcc gcacgtgcat
     7321 cgtgatgtcg cggttggccc gcaccgagcc gaagctcttg gtcagcccgg cgaggtcgag
     7381 cagcacctct ccgacgttgc cctccccgaa acccgacttc tccggcggga gctcgaggac
     7441 ggcatccgag gtgacgatgg cctcgaggtg ctcgggcgcg gtgtcggcgg ggaccggtgt
     7501 gggggtgttc tcgggggtgc tcatcactgg tctcccttga cgtacggctg tccgacggct
     7561 cccggtgccc gaacccgccc ggcggcgatg gccaggacga cgatcgtcac gacgaagggc
     7621 agggtgcccg tgagctgcgg cgggatgtcg atgctgccgc cggcctggag ctgcgaggcc
     7681 acggcgttga tgaagccgaa gaagatcgcc atcgccagcg cccgcagcgg acgccacgcg
     7741 cccatgatca tgatcgccag ggcgaggaac ccgcggccgg cgaccatgcc ccgctcgaag
     7801 gacgacgagg catccatcga caggtagacc ccggcacagc cggcgagcgt gcccgccagg
     7861 gtgacgttga tcagacgcag gcgctcgacg ttgatgccgg cggtgtcggc ggccgacggg
     7921 tactcaccga cggcgcgcgt gcgcagaccc cagcgggtgt ggaacagcgc gaagtgcaca
     7981 ccgatcgcgg cgaggatcgc gagcgtggcg aagatgctcg tgccggagaa gaagacctca
     8041 ccgatgagcg ggatcttcga cagcggccag atgtcgaact tcggcatcag cccgggcagc
     8101 agctgtcccg gctggtagta gaacgaggtg atgccggtgg cgatgatgtt gagcacgaca
     8161 cccgcgatga tctggtccat ccgccacttc acggtggtcc acgcgaggaa cccgccgagg
     8221 atcaggccgc tgccgatgcc ggcgagcacg cccacgagca gcgatccgct gtaggcggcg
     8281 gcgaagaacc cggcgaaggc cgcgacgagc atctgcccct cgatgccgat gttgacgaca
     8341 cccgagcgtt caccgatgac accgacgaga gcggccagga cgagcgggat ggcgtagccc
     8401 caggccgaga cgtacatgct cggggcgatg tcgttcttca ggtagtagaa ggcgtacgcc
     8461 gcgacgatga cggcgacgga cacgatcacg gtcgtgcggt tcttggcgat ccaggcaccc
     8521 atgtcagtta ccccagctcg tcgaggcggc ggtgaccttg tcggcgcggc tgcggaagat
     8581 ccacttgccg agcaccggga tggacaccat gagcaggatg atcgcgagca gcaggtcgac
     8641 gacctcgggg gcgatgcccg tcgcgaactg caggcccggg gctccggtgc gcaggatgcc
     8701 gacgagcaac gctgcgggga tcgtcgcgag cgggttggcg cgggccagca gggcgatggt
     8761 gatgccgtcg aacccgaggg tgccgccgat ggccggctcg aaccggtgca gcgtgccggc
     8821 cgcctcgagg gcgccggcga gaccggcgag gccaccaccg aagaccatcg acagggcgat
     8881 gatccggttg atgttgatgc cggcgtagcg ggcggcgtgc ttgttggcgc cgacggtgtt
     8941 gagcttgaag ccgagcgtcg tgcgcgacag catccaccag caggcgatgg ccagggccag
     9001 ggcgatgacg aacccgatgg ggatgccggc gacggcaccg acgcgggccg cgtcggggat
     9061 gagctcggtc tgcgggatgc cgctggtctt ggacccctcg gcctgcaggg ggccggtgac
     9121 aagccaccag gtcacgccgg ccgcgatgta gttgagcatg atcgtcgtga tgacctcgtg
     9181 cactccgcgg gtggccttga ggacaccggc gatccagccg atgaaggctc cggcgaccat
     9241 gccggcgacc atgccgcaca cggccccgag gaacccgagg ccaccagcgg cgtaggcgac
     9301 ccaggcgccg aacatggcgc cgaagatgag ctggccctga ccaccgatgt tgaacaggcc
     9361 cacgcgcagc gggatcacga cggcgaggcc ggtcagcacg agcggcgccg tcttctgcag
     9421 ggttcgggtc agcccgccgt cgacgaggag ggcctcacgg atgatcgtct cgtaggcgta
     9481 ccaggggctc accccctggt agcggatcag ggccgcaccg acgagcagtg cgatgacgag
     9541 ggcgacgagg atgacgacac cgctctggtg cgtcagcagg tacgccaggg gctgggccgg
     9601 cttcttgtca ccggaggttc ccccgctcga gggtggtggg gcggcggcct tgtcggccac
     9661 cgtgggtgtc tcgctcatac gactccaatg tcggtgggtg cccacagtgt ggcgcgtcgc
     9721 ggccccttgg caaagagtga cgcgacaggg aggtccacac gcgctcgggg gcgggcaccc
     9781 gtgctgggtg cccgcccccg gtgacgaggt ggttgggtcc cggaactagc ccgggcccga
     9841 ggtcacatcg gtcagagggt gacgccggtc tgcagggtgc cggcctccat ctgcttgacg
     9901 acctcgtcga ccttggcctt gacgtcggcc gggatgaccg actcgaagtc gtggaagggg
     9961 gcgaggccgg ccttgccgag ggtctggttc tcggcgaagt cgccgtcctt ggccgtgacc
    10021 agggtctcgg tgacaccctc ggtgatgagc ttcatcgccg aggtgatgag gcacggctgg
    10081 gccgcgggga cggtgtccca ctggtcggtg tcgacaccga tgcagaacac ctcggtgcct
    10141 gcacccttgg ccttggcgac ctcctggagg gcgccgttac cggtgttgcc accggcgccg
    10201 aagatgatgt ccgcaccctg ggcgagctgc ttcttggcct cgccagcacc ccacaccggg
    10261 tcgttgaagg cgttgtcgcc ggcggggtgg tacacggtgg tgacggtgat cgcggggttc
    10321 gcggccttcg cgccggcctc gaagcccttg gcgaacttct ggaccggggg gatctcgaga
    10381 ccgaggacct ggccgagctt gttcgtcttg gtcatcaggc ccgcgaggta accggcgccg
    10441 tagccagcct ggtcctcggg gaagatgagg ccggtgacgt tcttgccgga gaccgcggcc
    10501 gggtcgtagc cctggtcgac gccgatgaag cgggtgccgg ggttggcctt ggccgcctcg
    10561 agcgtggcgt cggtcatgag gaagccgacc gtgacgatgg cgtcgtactt cttgttggtg
    10621 aactgcgcca tgttggcggc gtagtccttg gcgtcctggg tctcgatgta cttgtagaag
    10681 cccttggtct gctcggcggc ggccagggcg ccctcgtgag cggactggtt gaacgacttg
    10741 tcgtccacct tgccggtgtc ggtgaccagg ccgatgcaga acacggcctc gtcggcgcag
    10801 ttgctgtcgt cggtgggggc gccgggagcg gccgacccgg aggcggcggg ggactcggtg
    10861 ttgccggagg tgccgccaca acccgcgagg gtgagggcga gcgcggcggg aaccgcgacc
    10921 aggtaacgcg tggacttcat gaaggttctc cttggagatg gggtcagaca cgtaaggcta
    10981 tgccgccaag cgcccctgac gggcagacct tgagtgtcgt tgcgctgttg ttacctcacc
    11041 agagggtgtc attgacgaca tcgacacgcc gcggttggca ggtgtcacca acccggaccg
    11101 gccgaccacc cggcatccac ggcgatggtg gtgccagtga tgcctgatgc ggctggtgag
    11161 gccagaaatg acacggtcgc cgcgacctcg gatgccgtga cggg
//
LOCUS       DAOSOC010000076        11178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_5807_length_11178_cov_9.573406, whole genome shotgun sequence.
ACCESSION   DAOSOC010000076 DAOSOC010000000
VERSION     DAOSOC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5807_length_11178_cov_9.573406"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PL014_07265"
     CDS             <1..628
                     /locus_tag="PL014_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56924.1"
                     /translation="DGSGGAAGPGGAAESGGAAGSAGARSEVAATTEGPAGPDGRATG
                     DRIVLDPDAYTRLSAVGRDVVLTHELAHVAVRSSVPGAPATWLAEGYADHVGYARAGL
                     GDGVLLAPLITAVREGRAPTELPDTSALQPTSGNLEVPYLAAWQAVDLIAQEHGEEAL
                     RELVRAAASTGTAADAEARTDAALETVLGSSREELTRAWRQRLETLAR"
     gene            complement(585..2048)
                     /locus_tag="PL014_07270"
     CDS             complement(585..2048)
                     /locus_tag="PL014_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56925.1"
                     /translation="MADAHEVSQTGGTGLRSESAAPEPGRSRAVAGTSAAVVLGLAWL
                     AWFGVLQWSVVRFRVPIVLTLTVCAALLAWWLARRFVIDVPRWLAPSVLLGSVVITLT
                     VPLFSYVRGGWLTAALAILTTGGLVCAVLLTRPGRATALAAYVVAVLTHTALAAVAVI
                     GDRAPKIDVWVVLQQGADALGRGENLYTQQWTDSPGVQDFFPYLPWMSVLTAPGRWLA
                     GDVRWAVLVWSLVLYAGLWALAKGHVERAAAVTAVLVLAPGTLTQVDQAWTEPVLAAA
                     IVWWAVLVRRGHAWWAIVPLALACASKQHLALLAPVLLVWRPFGVSRTLATGGLAGLL
                     MAPWVIADADAFVNDTVVTLLNFHPIRFANTWYLYALNEHGVTLPFAVTGAAMVGIVA
                     VAAYAVWRRRPDVAEVLRWLALVLAVANLVNKQGFYNQFWLVAALVAASLALPTGDSR
                     GATDADEAIDSDAADTPLPEGVTVSEPGSRAAAATPG"
     gene            complement(2041..3822)
                     /locus_tag="PL014_07275"
     CDS             complement(2041..3822)
                     /locus_tag="PL014_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HOT56926.1"
                     /translation="MSMPLVLPETSSGSLADLPGRQASANPGKVTFSVRSGSEWTDVT
                     AATFAADVAALAKGLMAAGIAPGEAVGIMSRTRYEWTVADFALWSAGAVPVPIYETSS
                     PDQVEWILADSAAVAVIVEDAAHLAAVEASRSRLPGLREVWVIGQGDIDSLSQDGDDV
                     DDADLAARRSALQRDSLATIIYTSGTTGRPKGVELTHGNFLTLAENTSEEIAEVVKGQ
                     GASTLLFLPLAHVFARFIEVLAVTAGVRMGHSSDLKALLDDFATFKPTFVLAVPRVFE
                     KIYNSAEAKAEAGGKGKIFAGATAAAIAWSQARDSGAVPLGLRVRHAVFDKLVYAKLR
                     DAMGGEIQYAVSGGAPLGTRLGHFFRGIGVTILEGYGLTETTAPATVNRPSAMRIGSV
                     GLPLPGVDVRIADDGEILLRGVNVFRGYHANDEATASSIQDGWFRTGDIGELDHDGFL
                     RITGRKKEILVTAGGKNVAPAVLEDALRAHPLISQCVVVGDGKPFIAALVTLDADMYP
                     GWAANNGMQGVPFAAARAHETVLAEVQKAVDAANTAVSKAESIRKFGILDGDFTLESG
                     HLTPSLKLKRNIVMRDFEDEVDALYGG"
     gene            3940..4383
                     /locus_tag="PL014_07280"
     CDS             3940..4383
                     /locus_tag="PL014_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT56927.1"
                     /translation="MADRTQSSIEIAAEPGEVLDVIADFERYPEWANEVKSVTLLTED
                     GDGWADQVEFRLDAGAIKDHYVLEYDWNVAEDGTGSVSWHLVRANLLKGLNGTYTLDR
                     VTGGTKVTYDLEVELTMPMIGLLKRKAEKHIINLALSELKKRVEG"
     gene            4392..5615
                     /locus_tag="PL014_07285"
     CDS             4392..5615
                     /locus_tag="PL014_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOT56928.1"
                     /translation="MPRVVLVTGKGGVGKTTTAAATAVRAALSGLRTLVMSTDAAHSL
                     GDALDVDLRTARTWDDSHAVEPGLHALAVGAHTAVEADWQVVRDYLLGVLDAMGVDPV
                     VADELTSLPGAEEVTALAALALQTQRGQWDLVVVDCAPTAETLRLLALPEVLGWHLDR
                     LLPAQRRLLTVLRPAASAATGVPVPGPEVLLVLRAWRDLMVEVRTLLTSPRASVRVVL
                     TPERVVIAESRRVLTSLSLHGYAVDAVVVNRVLPASAPGDTDPWRAAWTAAQASGLER
                     VRESFRGIPVLIAPYLAGEPIGVQALEALATSTTSERGAVPAIMDDVDVPVMRVAADG
                     DDFVLSLPLPLVSAGEVEMARRDDDLVIEVGGQRRVVTLPSVLRRCIVQNADVGNGVL
                     RVRFERDEEQWASGR"
     gene            5605..6144
                     /locus_tag="PL014_07290"
     CDS             5605..6144
                     /locus_tag="PL014_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56929.1"
                     /translation="MGADRWDEQVGSVADEAAKLLESLRRSAETASGAAPAGPAGAGE
                     PAGAEAHEPKGRHEGEGEGRRDGERPASEAGGHDPFCTWCPLCRTAEVVRSLSPETII
                     RLADLATLAATVLADFASARAADAPPNAPSDAGKSPSRRPAASAAPRPSPSRPIPVRD
                     AEPVSADGQGKDPKDTPSG"
     gene            6137..7114
                     /locus_tag="PL014_07295"
     CDS             6137..7114
                     /locus_tag="PL014_07295"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HOT56930.1"
                     /translation="MVEQGLAIGIDIGGTKVAGGVVDADGVVHERARRSTPHRSTSPR
                     VVEDTIVSVVEELLDSSTGPIVAVGIGAAGFVAADRATVVFAPHLSWRNEPLRDALAS
                     RVALPTFVDNDANAAVWAEHRFGAGRGESHLVMVNLGTGIGGGIVLDGQVIRGRHGIA
                     GEFGHMQVVPDGIRCECGNRGCWEQYASGNALVREARALFSAGSPVVSDLFDRVGGRP
                     EDLTGPLVTEAARDGDPLACELLAEIGRWLGLGIADLAAALDPGTFVIGGGVSAAGEL
                     LLEPARAAFRRQLTGRGYRPEATIVAAELGNEAGLVGAADLARTDALAR"
     gene            7126..7800
                     /locus_tag="PL014_07300"
     CDS             7126..7800
                     /locus_tag="PL014_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT56931.1"
                     /translation="MTTLRVATYNTRDFLDDHRLAARIVRAVAPDILCLQEVPRRLFA
                     TWRVRQFAAATGMTWPGRHRGSGGTTIFVSDRVRVLDCHHSRLPVRWPDRTRGYAVVR
                     VAAPGGTELTAVSVHLSLKPDERVVHTERILQALSDRGSLIIAGDLNEGDTGAAWRLI
                     DVPDRMRLVSPAAPTFPAKRPRRVLDVVFASPDLRVKPHRDVLLPDAVWAQASDHRAT
                     WVDLEL"
     gene            7913..8245
                     /locus_tag="PL014_07305"
     CDS             7913..8245
                     /locus_tag="PL014_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56932.1"
                     /translation="MSLPALEPPYDQLLATAERVARERPEVDVEMAREVFLEAATLLH
                     NGLALDGLDEHDASAVVAGLCQDLVSEDPGSAVLTRHEAAMESDGAFHDPAGVAASLL
                     VCAAVLQL"
     gene            complement(8266..8892)
                     /locus_tag="PL014_07310"
     CDS             complement(8266..8892)
                     /locus_tag="PL014_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT56933.1"
                     /translation="MTTVVVVDDHPVFRRGLVGLLTASGHEVVGQARGGLEALEVVAQ
                     AKPQVVLMDLSMPEGDGFEATARITAEHPDIAVVVVTLFSDEASVARALHAGAAGYLG
                     KHAEPEHLIAAVEAASSGALWIGRGVERPAFATRSGGEQAALPGGLTARELVVADLLA
                     RGLANPTIAARLGLSTKTVANYVSAILLKLGATDRVDAARMIRDHLAP"
     gene            complement(8889..10589)
                     /locus_tag="PL014_07315"
     CDS             complement(8889..10589)
                     /locus_tag="PL014_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT56934.1"
                     /translation="MTHDGLVEASGITVSVVVLVLAVLLLASLRRMPRGPRWVVVLAC
                     LLLYAVVVVPGAATLWFVAFPLLVGVYPDGVMTPRWLWVPVGMLAVAAFGDLVTAGMW
                     SESPWWGLVVNSQLLLLLTQVHRYRRRSSTQERESVRWVILGTLLTIASFAATQAAFG
                     SIGEGSTGSVVAAQLAVLPLLVGVAVGVLAPRALDVDEFLRATVVSLGTITALTVVVR
                     SLPAPDWVRLVVVAVIAAPVALTMTRVGDWLLYRGRPDPDRAVTRMLAALNTRTGKQE
                     TPRTVLTAFTDALYLDQGRISGSWFEPAALGPDTSTASGGEPFPVEYRGEVLAVLELP
                     PRRGESTLTRRDRRVVDRLIAQAAPALDAARTVGALRESRARTVAAREEERRRIRREL
                     HDDLGPTLSGIALSAAALAARTKDPDAAELHRELRLAVEQSRELAYGLRPPVLDDHGL
                     VAALHDRVGGPDVHIDAPEHLDLPASVDLAALRIIGEAVTNARKHGAAPIGIRLRLRH
                     GMLRLLVSDAGPGLPPDVRAGIGMMSISERTDEVGGTARYDRSAKGCHLVVDLPLEVS
                     "
     gene            complement(10618..>11178)
                     /locus_tag="PL014_07320"
     CDS             complement(10618..>11178)
                     /locus_tag="PL014_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56935.1"
                     /translation="GASDTPDMAALEANSWVGGFDAVLFSAMGVASLVMVLAVWRAMR
                     LRGAADGVLSTVGFAMALVSATAWFWVAGHGLSMYTSVGAGLADVSADSEIQAASLQA
                     SYLAVTAGLIVVGIGSIGWNVLLATTGRRAGLIGRPLSAVFGLFAAVPLYQAVVPFAT
                     PWPLIIAVLGSLVLGIALLLKSRKLR"
BASE COUNT         1617 a         3995 c         4108 g         1458 t
ORIGIN      
        1 ggacggctca ggcggggcag caggcccagg tggggcggca gaatccggcg gggcggctgg
       61 ctcggccggg gcacgcagcg aggtggcggc caccaccgag ggcccggccg gccccgacgg
      121 acgggcgacg ggggaccgca tcgtgctgga ccccgatgcc tacacccggc tgtccgccgt
      181 cggccgtgac gtcgtgctca cccatgagct cgcgcacgtc gcggtgcgct cgagcgtgcc
      241 cggagccccc gccacgtggc tggccgaggg ctacgccgac cacgtcggct acgcccgggc
      301 tggccttggc gacggcgtgc tgctcgcacc cctgatcacc gcggtgcgcg aggggcgcgc
      361 cccgaccgag ctgcctgaca cctccgcgct ccagccgacc tcggggaacc tcgaggtgcc
      421 ctacctcgcc gcgtggcagg ccgttgacct catcgcgcag gagcacggcg aggaggcgct
      481 gcgagagctg gtccgggccg cagcctccac cggcaccgcc gctgacgccg aggcccgcac
      541 ggatgccgcg ctcgagacgg tgctcggctc cagccgtgaa gagctcaccc gggcgtggcg
      601 gcagcggctc gagaccctgg ctcgctgacg gtcacccctt ccggcagggg agtgtcggcc
      661 gcgtcggagt cgatggcctc gtcggcgtcg gttgctcccc gggagtcacc ggtcggcagt
      721 gccaaggatg ctgccacgag ggccgcgacc agccagaact ggttgtagaa cccctgcttg
      781 ttgaccaggt tggccacggc cagcaccagg gcgagccagc gcagcacctc ggcgacgtca
      841 ggccgtcggc gccacacggc atacgccgcg accgccacga tgccgaccat ggcggcgccg
      901 gtcaccgcga acggcagggt caccccgtgc tcgttgaggg cgtagaggta ccaggtgttc
      961 gcgaaccgga tggggtggaa gttcagcagc gtgacgacgg tgtcgttgac gaaggcgtcg
     1021 gcatcggcga tgacccacgg cgccatgagc aaccctgcca ggcctccggt cgcgagggtt
     1081 ctcgagacgc cgaaaggccg ccagaccagg aggacgggag ccagcagtgc caggtgctgc
     1141 ttgctcgcgc acgcgagcgc cagcggcacg atcgcccacc acgcgtgccc ccggcgcacg
     1201 aggacggccc accacacgat tgcggccgcg agcacgggtt cggtccaggc ctggtcgacc
     1261 tgggtgaggg tgcccggggc gagcacgagc acggcggtca cggcggcggc ccgctcgacg
     1321 tgccccttgg ccagcgccca caggccggcg tacagcacga gcgaccacac gaggacggcc
     1381 caccgcacgt caccggcgag ccaccggccg ggagcggtga gcaccgacat ccacggcagg
     1441 taggggaaga agtcctgcac gccgggggag tcggtccact gctgggtgta gaggttctcg
     1501 ccgcgcccca gcgcatcggc cccctgctgg agcaccaccc agacgtcgat cttcggggcg
     1561 cggtcgccga tgacggcgac ggccgccagg gcggtgtggg tgagcaccgc gacgacgtag
     1621 gccgccaggg ccgtggcgcg gccgggtcgg gtcagcagga cggcgcagac cagccccccg
     1681 gtcgtgagga tcgccagggc cgccgtgagc caacctcccc gcacgtagct gaacagcggg
     1741 acggtgagcg tgatgacgac cgatccgagg agaacgctcg gggcgagcca acgcgggacg
     1801 tcgatgacga accggcgggc cagccaccac gcgagcaggg cagcgcacac ggtcagcgtc
     1861 agcacgatcg gcacccggaa ccgcacgacg ctccactgca gcacgccgaa ccacgcgagc
     1921 caggcaagcc cgagcaccac ggcggccgaa gtgccggcaa cggcacggga gcgaccgggt
     1981 tctggcgcag cgctctcgct ccgcagaccg gtgccgccgg tctgcgacac ctcgtgggcg
     2041 tcagccaccg tagagggcgt cgacctcgtc ctcgaagtcg cgcatgacga tgttgcgctt
     2101 gagcttcagg gacggggtga ggtgaccgga ctcgagggtg aagtcgccgt cgaggatgcc
     2161 gaacttgcgg atcgactcgg ccttggagac cgcggtgttc gcggcgtcga ccgccttctg
     2221 aacctcggcg agcacggtct cgtgcgcgcg ggcagcggcg aacggcaccc cctgcatgcc
     2281 gttgttcgct gcccagccgg ggtacatgtc cgcgtcgagg gtcacgaggg ctgcgatgaa
     2341 gggcttgccg tcgccgacga cgacgcactg gctgatgagc gggtgggccc gcagggcgtc
     2401 ctcgaggacc gcgggggcga cgttcttgcc accggcggtg acgaggatct ccttctttcg
     2461 accggtgatc cgcaggaagc cgtcgtggtc gagctcaccg atgtcgccgg tgcggaacca
     2521 cccgtcctgg atcgaggatg ccgtcgcctc gtcgttcgcg tggtagccgc ggaagacgtt
     2581 gacgcccctc agcaggatct cgccgtcgtc ggcgatcctc acgtcgacgc cgggcagggg
     2641 gaggccgacc gagccgatcc gcatcgccga cgggcggttc acagtggccg gcgccgtggt
     2701 ctcggtcagg ccgtagccct cgaggatcgt cacgccgatg ccccggaaga agtggccgag
     2761 ccgcgtgccg agcggtgcac caccggacac ggcgtactgg atctcgccgc ccatcgcgtc
     2821 gcgcagcttc gcgtagacga gcttgtcgaa caccgcgtgg cgcacgcgca ggccgagcgg
     2881 aaccgcaccc gagtcccggg cctgcgacca cgcgatggcc gccgctgtgg ctccggcgaa
     2941 gatcttgccc ttgccgccgg cctcggcctt ggcctcggcg gagttgtaga tcttctcgaa
     3001 cacgcgcggc acggccagca cgaacgtcgg cttgaaggtg gcgaagtcgt cgagcagggc
     3061 cttgaggtcg gaggagtgcc ccatccgcac cccggccgtc accgccagca cttcgatgaa
     3121 ccgggcgaag acgtgggcga ggggcaggaa gagcagggtg gaggcgccct ggcccttgac
     3181 gacctcggcg atctcctcgc tggtgttctc ggccagggtg aggaagttgc cgtgggtcag
     3241 ctcgacaccc ttcgggcggc cggtggtgcc cgaggtgtag atgatcgtcg cgaggctgtc
     3301 gcgctgaagg gctgagcgac gagccgccag gtcggcgtcg tcgacgtcgt cgccgtcctg
     3361 gctcaggctg tcgatgtccc cctggccgat gacccagacc tcccgaaggc cgggcaggcg
     3421 cgaacgggac gcctcgacgg cggcgaggtg ggcggcatcc tcgacgatga ccgcgacggc
     3481 ggcggagtcc gcgaggatcc actcgacctg gtcgggggag gacgtctcgt agatagggac
     3541 cgggacggcc ccggccgacc agagggcgaa gtcggccacc gtccactcgt agcgggtgcg
     3601 gctcatgatg ccgacggcct cgccgggggc gatgccggcc gccatgaggc ccttggcgag
     3661 agcggccacg tcggcggcga acgtggccgc ggtgacgtcg gtccactccg accccgaccg
     3721 caccgagaag gtcaccttgc cggggttggc gctcgcctga cgacccggca ggtcggccag
     3781 gctcccgctg gaggtctcgg gcaggacgag cggcatggac atctcttgca cggtcgactc
     3841 cttgggggct gtagaagggg tttgaaggcc cactgtatga ccccgcgggc gggggaccag
     3901 cgatccccgg gacgcgcggt gcgggtagcc tgccgtttca tggccgaccg cacacagtcc
     3961 tcgatcgaga tcgccgccga acccggggag gtcctcgacg tcatcgccga cttcgagcgc
     4021 tacccggagt gggcgaacga ggtcaagtcc gtgaccctgc tgaccgagga cggcgacggc
     4081 tgggccgacc aggtggagtt ccggctcgac gcgggggcca tcaaggacca ctacgtgctc
     4141 gagtacgact ggaacgtcgc cgaggacggc acgggcagcg tgtcctggca cctcgtgcgg
     4201 gccaacctcc tgaagggcct gaacggcacc tacacgctcg accgtgtcac cggcggcacc
     4261 aaggtcacct acgacctcga ggtcgagctg acgatgccga tgatcggcct gctcaagcgc
     4321 aaggccgaga agcacatcat caacctggcc ctgtccgagc tcaagaagcg cgtcgagggc
     4381 tgacctccac catgccccgc gtcgtcctgg tgaccggcaa gggcggcgtc ggcaagacga
     4441 cgaccgccgc cgccacggcc gtgcgggctg cgctgtcggg tctgcggacc ctggtcatgt
     4501 ccacggatgc cgcacacagc ctcggtgacg cgctcgacgt cgacctgcgc accgcccgta
     4561 cctgggacga cagccacgcg gtcgaacccg ggttgcacgc cctcgccgtc ggcgcacaca
     4621 ccgctgtcga ggccgactgg caggtggtgc gcgactacct gctgggcgtg ctggacgcca
     4681 tgggcgtgga ccccgtcgtc gccgacgagc tgacctcgct gcccggtgcg gaggaggtca
     4741 cggcgctggc cgccctcgcc ctgcagaccc agcgcggcca gtgggacctc gtcgtcgtcg
     4801 actgcgcccc caccgcggag acccttcggc tgctcgccct gcccgaggtc ctcggctggc
     4861 acctggatcg cctgctgccc gcacaacgac gactgctcac ggtgctgcgc ccggcggcat
     4921 ccgccgccac gggtgtgccg gtgccgggcc cggaggtgct gctcgtcctg cgtgcctggc
     4981 gcgacctcat ggtcgaggtc cgcaccctgc tcacctcgcc ccgggccagc gtgcgcgtgg
     5041 tgctcacccc ggagcgcgtc gtcatcgccg agagccgtcg ggtgctcacc tcgctctcgc
     5101 tccacggcta cgccgtcgat gccgtcgtcg tgaaccgggt gctgcccgcg tccgcgccgg
     5161 gcgacaccga cccgtggcgg gcggcgtgga ccgcggccca ggccagcggg ttggaacggg
     5221 tgcgggagtc cttccgcggc atccccgtcc tcatcgcccc ctacctcgcc ggcgagccga
     5281 tcggggtgca ggcactcgag gcgctcgcca cctcgacgac gtccgaacgt ggcgcggtgc
     5341 cggcgatcat ggacgacgtc gacgtgccgg tcatgcgggt cgcggcggac ggtgacgact
     5401 tcgtgctcag cctgccgctg ccgctcgtgt cggcgggtga ggtggagatg gcccgccgtg
     5461 acgacgacct ggtcatcgag gtgggcgggc agcggcgcgt cgtcaccctc ccgtcggtgc
     5521 tgcgccggtg catcgtccag aatgccgatg tcggcaacgg agtgctgcgg gtgcggttcg
     5581 aacgggacga ggagcagtgg gccagtgggc gctgatcgct gggatgagca ggtcgggtcg
     5641 gtcgccgacg aggccgccaa gctgctcgag agcctgcgcc ggtcggcgga gaccgccagc
     5701 ggcgccgccc cggccggccc cgctggcgcc ggcgagcccg ctggcgcaga ggcgcacgag
     5761 cccaagggtc gacatgaggg tgaaggtgag ggtcgacgcg acggtgagcg cccggcatcc
     5821 gaggcaggtg ggcacgatcc cttctgcacc tggtgcccgc tgtgccgcac cgccgaggtc
     5881 gtgcgctcgc tgagccccga gacgatcatc cgcctggccg acctcgcgac cctggccgcc
     5941 acggtcctgg ccgatttcgc gagcgctcgt gcggcggacg cacccccgaa cgccccctcg
     6001 gatgccggga agtcgccgtc gcggcgaccc gcggcgtccg cggcgccgcg acccagcccc
     6061 agccgcccca tcccggtgag ggacgccgag cccgtcagcg ccgacggaca gggcaaggac
     6121 ccgaaggata cgcccagtgg ttgagcaggg tctggcgatc ggcatcgaca tcggcggcac
     6181 gaaggtcgct ggcggggtcg tcgacgccga cggggtcgtc cacgagcggg cccgtcgcag
     6241 cacccctcac cggtcgacga gcccgcgcgt cgtcgaggac accatcgtct cggtcgtcga
     6301 ggagctgctg gacagcagca cggggccgat cgtcgcggtc ggcatcggtg ccgccggctt
     6361 cgtcgcggcc gaccgcgcga ccgtggtctt tgccccacac ctgtcctggc gcaacgaacc
     6421 gctgcgcgac gccctcgcca gccgggtggc gctgccgacc ttcgtcgaca acgacgccaa
     6481 cgccgcggtg tgggccgagc accgcttcgg tgccgggcgc ggcgagagcc acctcgtcat
     6541 ggtcaacctc ggcacgggga tcggcggggg catcgtgctc gacggtcagg tcatccgtgg
     6601 ccggcacggc atcgcgggcg agttcgggca catgcaggtc gtgcccgacg gcatccggtg
     6661 tgagtgcggc aaccgggggt gctgggagca gtacgcctcc ggcaacgcgc tcgtgcggga
     6721 ggcccgggcg ctgttctccg cgggaagtcc cgtcgtctcc gacctcttcg accgggtcgg
     6781 tggccgacct gaagacctca ccggcccgct cgtcaccgag gccgcgcgcg acggcgaccc
     6841 gctcgcctgc gagctgctgg ccgagatcgg gcgctggctg gggctcggca tcgccgacct
     6901 cgccgctgcc ctggaccccg gcaccttcgt catcggcggg ggtgtgagcg cagccggcga
     6961 gctgctgctc gagccggcgc gggcggcctt ccgtcgccag ctgaccggcc gtgggtaccg
     7021 cccggaggcg accatcgtcg cggccgagct gggcaacgag gccgggctcg tcggtgccgc
     7081 cgacctcgcc cgcaccgacg ccctggcgcg ctgaggtcga cctcggtgac cacgctgcgg
     7141 gtggcgacct acaacacgcg cgacttcctc gacgaccacc ggctggccgc ccggatcgtg
     7201 cgcgcggtgg cgcccgacat cctgtgcctg caggaggttc ctcgccgact cttcgcgacc
     7261 tggcgcgtgc gtcagttcgc ggccgcgacc ggcatgacct ggcccggccg tcaccggggc
     7321 agcggaggca cgacgatctt cgtctccgac cgggtgcggg tgctcgactg tcaccactcc
     7381 cggctgccgg tgcgctggcc ggaccgcacc cgtggctacg ccgtcgtgcg ggtcgccgcg
     7441 ccgggcggga ccgagttgac ggccgtgtcg gtgcacctca gcctgaagcc cgacgagcgg
     7501 gtcgtccaca ccgaacgcat cctgcaggcg ctgtcggacc gcggctcgct catcatcgcc
     7561 ggcgacctca acgagggcga caccggcgcc gcgtggcggc tcatcgacgt ccctgaccgg
     7621 atgaggctgg tgtccccggc ggccccgacg ttcccggcga agcggccgcg gcgggtgctc
     7681 gacgtggtgt tcgcgtcacc ggacctgcgc gtcaaaccgc accgcgacgt gctgttgccg
     7741 gatgccgtgt gggcgcaggc gagcgaccac cgggccacct gggtcgacct cgagctctga
     7801 gcgccggccc ggcactccat cgcccaccgg tgcatcgccc accgtcgggg agcccactcg
     7861 tggcgagcaa actcggttgt cgccgggagg tggccactgt caggctctgc ccatgtccct
     7921 ccctgccctc gagccgccgt acgaccagct gctcgccacc gccgagaggg tcgcccggga
     7981 gcggcccgag gtcgatgtcg agatggcccg tgaggtcttc ctcgaggcgg cgaccctgct
     8041 ccacaacggc ctggctctcg acgggctcga cgagcacgat gcgagcgccg tcgtcgccgg
     8101 gctgtgtcag gatctcgtct ccgaggaccc cggcagtgcc gtgctcaccc gccacgaggc
     8161 cgcgatggag tccgacggtg cgttccacga cccggccggt gtcgcggcat ccctgctcgt
     8221 gtgcgcagcc gtcctccagc tgtgaggacc gtgtgtgtcg tggtgtcagg gcgcgaggtg
     8281 gtcgcggatc atcctcgctg catccacccg atccgtcgcc cccagcttga gcaggatcgc
     8341 gctcacgtag ttcgcgacgg tcttggtcga caggccgagg cgggcggcga tcgtcgggtt
     8401 ggccagcccg cgggcgagca ggtccgcgac cacgagctcg cgggccgtga gtcctccggg
     8461 aagtgcagcc tgctcaccgc ctgaccgcgt ggcgaacgcg ggccgctcga cgccgcggcc
     8521 gatccacaac gccccggaac tggccgcctc cacggcggcg atgaggtgct cgggctccgc
     8581 gtgcttgccg agatacccgg ccgcccccgc atgcagggcc cgggcgaccg aggcctcatc
     8641 ggagaagagg gtgacgacga ccacggcgat gtcggggtgt tcggcggtga tcctggccgt
     8701 ggcctcgaac ccgtcgcctt cgggcattga caggtccatg agcacgacct gcggcttggc
     8761 ctgggcgacc acctcgagcg cctcgaggcc gcccctggcc tgacccacga cctcgtgccc
     8821 cgaggccgtc agcagaccca ccaacccgcg gcggaagacg gggtggtcgt cgacgacgac
     8881 gacggtggtc atgacacctc caggggtagg tcgaccacca ggtggcagcc ctttgcgctc
     8941 cggtcgtagc gggcggtgcc gccgacctcg tcggtgcgct ccgatatcga catcatgccg
     9001 atgcccgcgc gcacgtccgg gggcaggccg ggcccggcgt cactgaccag caggcggagc
     9061 atgccgtggc gcaaccgcaa ccggatgccg atgggtgcgg caccgtgttt gcgcgcgttg
     9121 gtcacggcct ccccgatgat ccgaagggcc gcgaggtcga ccgaggccgg gaggtcgagg
     9181 tgctccggcg cgtcgatgtg gacgtcgggg ccacccaccc ggtcgtgcag ggcagccacg
     9241 agcccgtggt cgtcgagcac cggcgggcgc agcccgtagg cgagctcgcg ggactgctcg
     9301 acggccagcc gcagctcccg gtgcagctcg gcggcgtccg ggtccttggt gcgggccgcc
     9361 agtgctgccg cgctcagcgc gatgccggag agggtcgggc cgaggtcgtc gtgcagctcc
     9421 cggcggatcc tgcgtcgctc ctcctcgcgc gcggcgacgg tgcgggcccg ggactcgcgc
     9481 agcgccccga ccgtgcgcgc ggcgtcgagc gcgggggccg cctgggcgat gagcctgtcg
     9541 accactcggc ggtcacggcg ggtgagggtg ctctcgccgc gccggggcgg cagctcgagg
     9601 acggccagca cctcccctcg gtactcgacg ggaaagggct caccgccgga tgccgtggag
     9661 gtgtccggtc ccagcgcggc gggctcgaac cacgagccgc tgatccgccc ttggtccagg
     9721 tacagggcgt cggtgaaggc ggtgagcacg gtcctcgggg tctcctgctt gccggtgcgg
     9781 gtgttgagcg cagccaacat ccgggtgacg gcgcggtctg gatcggggcg cccccggtag
     9841 aggagccagt cgccgacgcg ggtcatcgtc agggccaccg gtgcggcgat gacggcgacg
     9901 acgacgaggc gcacccagtc aggggccggt agggaccgga cgaccacggt gagcgccgtg
     9961 atggtgccca ggctcaccac cgtcgcccgc aggaactcat cgacgtcgag cgctcgcggg
    10021 gcgaggaccc ccacggcaac gccgacgagc agcggcagca cggccagctg ggcggcgacc
    10081 acggagccgg tcgagccctc gccgatggac ccgaaggcgg cctgggtcgc cgcgaacgac
    10141 gcgatcgtca gcaacgtccc gaggatcacc cagcgcaccg actcccgctc ctgcgtggag
    10201 gatcggcggc ggtaccggtg gacctgggtg aggagcagga gaagctggga gttcacgacc
    10261 aggccccacc acgggctctc gctccacatg ccggccgtga cgaggtcgcc gaacgccgcg
    10321 acggcgagca tgcccaccgg cacccacagc caccgcggtg tcatgacgcc atccgggtac
    10381 acaccgacga gcagggggaa ggccacgaac cacagggtgg ctgcaccggg cacgacgacg
    10441 acggcataca ggagtaggca ggcgagcacg accacccagc gggggccgcg cggcatccgg
    10501 cgcagtgacg cgagcaggag cacggccagg acgagcacga ccacgctgac cgtgatcccg
    10561 ctcgcctcga ccaacccgtc gtgggtcatg ggccgatgat gacaccggcg ggctgcgtca
    10621 ccgcagtttc cgggacttca ggagcagcgc gatgccgagg accagcgacc cgaggaccgc
    10681 gatgatgagc ggccacggcg tcgcgaaggg caccaccgcc tggtagagcg ggaccgcggc
    10741 gaacaggccg aacacggcgc tgagcggccg gccgatgagt ccggccctcc ggccggtggt
    10801 ggccagcagg acgttccagc cgatcgaccc gatcccgacg acgatcagcc ccgccgtgac
    10861 ggccaggtag gaggcctgga gcgaggccgc ctggatctcg ctgtccgccg agacgtcagc
    10921 cagacccgcg cccaccgagg tgtacatgct cagcccgtgc ccggcgaccc agaaccacgc
    10981 agtggcgctc acgagtgcca tggcgaaccc gacggtgctg aggacgccat ccgcggcacc
    11041 gcgcaggcgc atggcgcgcc acacggccag caccatgacg agtgaggcca cgcccatggc
    11101 ggagaacagc acggcgtcga agccgccgac ccacgagttg gcctcgagcg ctgccatgtc
    11161 cggggtgtcg ctggcacc
//
LOCUS       DAOSOC010000077        10873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_6026_length_10873_cov_8.486134, whole genome shotgun sequence.
ACCESSION   DAOSOC010000077 DAOSOC010000000
VERSION     DAOSOC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6026_length_10873_cov_8.486134"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PL014_07325"
     CDS             <1..827
                     /locus_tag="PL014_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOT56936.1"
                     /translation="SRHPEKAPWVKLTVEPFPRLSVVRAVADDGARYAGPFSSRAAAE
                     AAIAAVHEVLPLRQCLERLSPTRPRSACALADMGRCGAPCTGAQSVDDYALIVDRAAD
                     LLSGGTRDLLTALRDRMAELSAQQRFEDAQVVRDRLVALVRGTSRSQRLDPLARSREL
                     VAARRTLLGGWEVICVRHGRLAGSTTSPRGADPMPYIEALRASAEVVAPALPPATAAT
                     AEESEKILRWLESPGVRIVDIDGVWTCPVGGAVPVRAELEPLESARREVAGFAGSR"
     gene            939..1220
                     /locus_tag="PL014_07330"
     CDS             939..1220
                     /locus_tag="PL014_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOT56937.1"
                     /translation="MITAIVLITADVDRIPEVAQEIADLDGVTEVYSVAGDADLIAMV
                     RVRRHEDLADTIADRLNKVTGVTGTNTHIAFRTYSAHDLEAAFSLGLEN"
     gene            complement(1305..2369)
                     /gene="trpD"
                     /locus_tag="PL014_07335"
     CDS             complement(1305..2369)
                     /gene="trpD"
                     /locus_tag="PL014_07335"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOT56938.1"
                     /translation="MTLTDPARATWPDLLTALLRREDLSTEQAGWAMAEIMSGEATPV
                     QVAGFLVALRAKGETVAELRGIADVMLEHANRIDVPGPSIDIVGTGGDRMHSVNISTM
                     ASIVVAATGLRVVKHGNRAASSSSGSADVLEALGVSLTLAPEQVAAVAQEAGITFCFA
                     NAFHPSMRHAAVARRDLGVGTAFNALGPLTNPGQPTYAAVGVADARIAPLIAGVFAGR
                     GRAAAVFRGDDGLDELTLATTSTVWWVRDGDVTELSLDPSDLGLELSPVEALRGGDAA
                     HNAQVVRDLLAGEVGPVRDAVVLNAGMALATASGLGDDVSSAALTRAVDEGMRRARAA
                     IDDGSAARLLERWVSATATA"
     gene            complement(2375..2824)
                     /locus_tag="PL014_07340"
     CDS             complement(2375..2824)
                     /locus_tag="PL014_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56939.1"
                     /translation="MTSSHGTDTSAQAPESDGIHRVSVLLYSDDVTTRDAVRAAAGRR
                     PARDVEIVRWHECATAEAVVEAMDAGGYDVVILDGEASPLGGLGLCRQLKNEIFECPP
                     ILVLTGRPQDGWLAGWSLADESVSHPLDPIVVSEAVAALARTRVAVG"
     gene            2978..3655
                     /locus_tag="PL014_07345"
     CDS             2978..3655
                     /locus_tag="PL014_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HOT56940.1"
                     /translation="MAFVATQTASQTRPAGPVPSIPGQGPATRPNLVAVGTMVWLSSE
                     LMFFAALFAFYFTIRGHEPQLWAENTAILNIPFALANTIVLVISSVWCQLGVLQAEKG
                     VVARPAGTKLTKVSAWGMREWYVLTYIFGAVFIAGQVYEYAHLVAENVTLSSDAYGSV
                     FYLATGFHGMHVTGGLIAFLLIIGRTFTTRRYTHAQATGAVVTSYYWHFVDVVWIALF
                     IMIYVLK"
     gene            3714..4526
                     /locus_tag="PL014_07350"
     CDS             3714..4526
                     /locus_tag="PL014_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOT56941.1"
                     /translation="MNSLAARRRHPAAIAVLIILGLFLTGAVYAAFAPKPADATTKAL
                     PAQTVEEGKALFRANCASCHGLNAEGRATGNGDKVAGPALAGVGAGAVEFQMGTGRMP
                     LAGPKVQAPARGEVKFSQDQIDAIGAYIASLSPGPERPSAEAIDPTKGDPAKGGELFR
                     VNCAMCHNFAGAGGALTRGKYAPALTGTSDEHIYLAMTTGPQSMPVFNDSNLSPEAKR
                     DIIAFLNTIEEQPKQGGLSLGSMGPVSEGLFAWVFGLGIFVACAVWLGSKSA"
     gene            4578..5729
                     /locus_tag="PL014_07355"
     CDS             4578..5729
                     /locus_tag="PL014_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOT56942.1"
                     /translation="MNEQETHHPGAEVEPASTSSTAVRLDEGHIIGTGGVPERFTNPG
                     LPAHVARAADLDEAAARRAEKQVATLFGLSILGTLAFIVLYFAVDLDQTVFVPGIGVT
                     NASNLALGTTMALGMLGIGFGAVHWAKTLMPDEEVVEERHPMRASDESRERVVKVLAE
                     GGEQSQITRRPLIKYTMGGALGLFAVPLVLQVAGSLGPMPQKSLSRTFWNGGPSGPGE
                     DPEFRPLRLMRDPEGTPVKAEDVTIGSVFHIMPEGLLPNPDDPEAEYLEKHILEEKAK
                     AAVILIRLDEDLIESQKQRDWGHQGIVAYSKICTHVGCPVGLYEQQTHHLLCPCHQST
                     FDVTQDCKVIFGPAKRPLPQLKITVDDEGYLVSAAPFAEAVGPSFWERG"
     gene            5729..7462
                     /locus_tag="PL014_07360"
     CDS             5729..7462
                     /locus_tag="PL014_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HOT56943.1"
                     /translation="MSTTTARPADALREGDTPVEAAPSGAARRVGGIAGWVDDRTGAA
                     KPVGYLMKKVFPDHWSFMLGEIAMYSMIICLITGAFLTFWFVPSAGHTVYDGSFVPLR
                     GVGMSEAYASTLNISFDIKGGLIIRQIHHWAALMFIVALTVHMFRVFFTGAFRKPREI
                     NWVIGTVLALLAIVEGFAGYSLPDDLLSGTGIRAMAGFVQTAPVIGTYLVYGIFGGPF
                     PGEMIIPRLYSVHILLLPAILIGLFTAHIGLVFVQKHTQYPGPGRTNENVVGFPVMPV
                     YAAKAGGFFFIVFGVLAMISALVQINPVWAYGPYDPSPVTAGSQPDWYMGFADGALRL
                     LPGWLEFEFWGYTLSLNIALGALVLLPLVYTVLGIYPFVERWVTKDDREHHLLDRPRN
                     NPVRTGIGMAGITAYSVFMFAAGNDIMAIKLGMSINDITWFFRIGLFVLPPLMFWVTK
                     RICLSLQRRDRDTVLHGRETGTIIRTPDGKYFERHDTELTADQWVLVQHDPVAPLELE
                     TEVDEHGVARKTSRLAKAKVALSRFYFADAVNPVTPAELAAAHHHGEEHEAIEAAPSA
                     DESEPAQVGRH"
     gene            complement(7637..7909)
                     /locus_tag="PL014_07365"
     CDS             complement(7637..7909)
                     /locus_tag="PL014_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOT56944.1"
                     /translation="MHVTVRVRPGASRTRVGGRWGDGEVLGVSVSARAVDGAATAAVC
                     AAVAGAFGVRPREVSLVAGERSRTKVLEVCVDADQGRRTLEALLAR"
     gene            7965..9377
                     /locus_tag="PL014_07370"
     CDS             7965..9377
                     /locus_tag="PL014_07370"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT56945.1"
                     /translation="MSHEDTGELPRSTPESQGVPSGAVLAMLRRWETSGCEPHSVVIV
                     RHGHVVAQGWWAPYRPEHQQLLYSVSKTFLAVAVALAEDDGLLGRGERLVDVFPEAAA
                     TAGPRASEITVEDCLRMSTGHHEDTLDTVPGLLGSGDGAAALFLAAEPEHEPGSWFVY
                     HNGASLMLALAVQRRTGQRLLHYLRPRLLEPLGITSASWRSAAGGDLGFSGLHTTTDA
                     VARLGVLLLDDGVWQGTRLLPSGWVGTASSALSNTAHHPGTPDWQQGYGYQLWRSRHD
                     GFRADGAYGQFSLIHPRHDLVVAVTACTDDAQPILEAVWEELIPALTDTPSPADPEAV
                     HELSQALDTAVLPAVGSTCEPAVPGPWVFTHEPTPEHPRLTRVEVRPHENSWLLVVDD
                     GEPLPITCGDGRWPDAAGAPFVAAGGWVSPDVFEATIVAVQTPHSLHLHCTDGVASAR
                     WRGYPLHGPCLSWQREPDGD"
     gene            9379..9732
                     /locus_tag="PL014_07375"
     CDS             9379..9732
                     /locus_tag="PL014_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOT56946.1"
                     /translation="MRPVTHDEFDAIVQDALDDVPAELMAMLDNVVFFVEDEPSADDP
                     DLLGIYEGTPLTERGEWWGQGSLPDRITLFRGPLTRMCADAEELREEIAVTVVHEIAH
                     HFGIEEETLHDLGWG"
     gene            complement(9874..10287)
                     /locus_tag="PL014_07380"
     CDS             complement(9874..10287)
                     /locus_tag="PL014_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HOT56947.1"
                     /translation="MLAMERIGALLGVFAFLMATIYGFWTNSTELGIEWVGVIGLILG
                     GLLGFMITWYLGMTRRKLEVDPSDDPLGDIDEIQGDYGFFSPHSWWPLFLAAAAAVCF
                     LGLAVGWWVFIIGAFFAVPALIGWTFEYWKGEHAL"
     gene            complement(10293..>10873)
                     /locus_tag="PL014_07385"
     CDS             complement(10293..>10873)
                     /locus_tag="PL014_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOT56948.1"
                     /translation="AHFHYVVFGTVVFAMFAGFYFWWPKLTGRMLDERLGKIHFWLLF
                     IGFHTTFLVQHLIGIQGMPRRYADYMPEDGFTLGNQISTVGSFVLAVSMLPFMYNVWK
                     TWKTAPLVEVDDPWGYGASLEWATSCPPPRHNFNSIPRIRSERPAFDLHHPEAAPAGV
                     HDAAVADDNVLVSALGPADLGGNAFADEKKEG"
BASE COUNT         1667 a         4000 c         3592 g         1614 t
ORIGIN      
        1 ggtcacggca tcccgagaag gccccgtggg tcaagctgac cgtcgagccg ttcccccggc
       61 tgtcggtggt gcgtgcggtc gccgatgacg gcgcccgcta cgccgggccg ttctcgtcgc
      121 gcgcggccgc agaggcagcg atcgcggccg tccacgaggt gctcccgctg cgccagtgcc
      181 tcgagcggct gtcccccacc cggccccgat ccgcctgcgc cctcgcggac atgggccgct
      241 gcggtgcgcc ctgcaccggc gcccagtcgg tcgacgacta cgcgctgatc gtcgaccgcg
      301 cggctgacct gctctccggc ggaacccgcg acctgctcac cgccctgcgc gaccggatgg
      361 ccgagctctc cgcgcagcag cgcttcgagg acgcccaggt ggtccgcgac cggctcgtcg
      421 ccctcgtgcg gggcacctct cggtcgcagc gcctggatcc gctggcacgc agccgcgagc
      481 tcgtcgccgc ccggcgcacc ctgctcggcg gctgggaggt catctgcgtg cggcacggcc
      541 ggctcgccgg cagcacgacc tcaccccgtg gcgccgaccc gatgccctac atcgaggcac
      601 tgcgggccag cgccgaggtc gtcgcccccg cgctgccacc cgcgacggcg gccaccgccg
      661 aggagagcga gaagatcctg cgctggctcg aatcacccgg ggtgcgcatc gtcgacatcg
      721 acggggtctg gacctgcccg gtcggtgggg cggtacccgt gcgcgccgag ctcgaaccgc
      781 tggagagcgc ccgacgtgag gtcgctggct tcgccggatc gcgctgaggc tcgttgcaga
      841 ccaccaccga gggcatgcag tgaaccctcc gctccgcatc cgacgcccgc tcggcacggc
      901 atccggtccc tcgtgaccgc cccggctacg ctgacgtcgt gatcaccgcc atcgtcctca
      961 tcaccgccga cgtcgaccgc atccccgagg tcgcgcagga gatcgccgac ctcgacggcg
     1021 tcaccgaggt ctactcggtg gccggcgacg ccgacctcat cgccatggtg cgggtgcggc
     1081 ggcacgagga tctcgccgac acgatcgccg accggctcaa caaggtcacc ggggtgacgg
     1141 gcacgaacac ccacatcgcg ttccgcacct actccgcgca cgacctcgag gcggccttca
     1201 gcctcggcct cgagaactga cctgtcgaga actgaccgct cgagacctgc ccgcgccgaa
     1261 acggattgag ccagacctga ccgagcgcgg tctggccgac ccggtcaggc ggtagcggtc
     1321 gcgctgaccc agcgctccag cagccttgcg gcagacccgt cgtcgatggc ggcgcgggcc
     1381 cgccgcatcc cctcgtcgac ggccctcgtc agcgcggccg acgacacgtc gtcaccgaga
     1441 ccggatgccg tggcgagagc catgcccgcg ttgagcacca ccgcgtcgcg caccgggccg
     1501 acctccccgg ccaacaggtc ccgcaccacc tgggcgttgt gcgcggcgtc tcctccgcgc
     1561 agggcctcca ccgggctgag ctccaggccg agatcgctcg ggtcgaggct gagctcggtg
     1621 acgtcgccgt cgcgcaccca ccacacggtg gacgtcgtcg ccagggtcag ctcgtcgagg
     1681 ccgtcgtcgc cgcgaaagac cgccgcggcg cggccacggc cggcgaacac accggcgatg
     1741 agcggggcga tccgagcatc ggcgacaccc accgcggcgt acgtcggctg gccggggttg
     1801 gtgagcgggc ccagcgcgtt gaacgcggtg ccgaccccga ggtcgcgacg ggcgacggcg
     1861 gcgtgacgca tcgacgggtg gaacgcgttg gcgaagcaga acgtgatgcc ggcctcctgc
     1921 gccacggcgg cgacctgctc gggggccaac gtcagcgaca cgccgagcgc ctcgagcacg
     1981 tcggcagagc ccgagctcga ggacgccgcc cggtttccgt gcttcacgac ccgcagaccg
     2041 gtcgccgcga cgacgatcga ggccatcgtc gagatgttga ccgagtgcat gcggtccccg
     2101 ccggtgccga cgatgtcgat gctcggaccc ggcacgtcga tccggttcgc gtgctcgagc
     2161 atgacgtcgg cgatgccgcg cagctcggcc acggtctcgc ccttggcacg cagggcgacg
     2221 aggaaccccg cgacctggac cggggtggcc tcccccgaca tgatctcggc cattgcccag
     2281 ccggcctgct cggtgctgag gtcctcccgg cgcaggagtg cggtcaagag gtccggccag
     2341 gttgccctgg ccggatcggt gagggtcacg acgctcagcc gaccgcgacc cgggttcgcg
     2401 ccagggcagc cacggcctcg gagacgacga tcgggtcgag cgggtgtgac acggactcat
     2461 cggcgagcga ccacccggcc agccacccgt cctgcgggcg cccggtgagc acgaggatcg
     2521 gcgggcactc gaagatctcg ttcttcagct gacgacacag gccgagcccg ccgagggggg
     2581 aggcctcgcc gtcgaggatc acgacgtcgt acccgccggc atccatggcc tcgacgacgg
     2641 cctcggcggt cgcgcactcg tgccaccgga cgatctcgac gtcgcgggcg gggcggcggc
     2701 cggcagcggc acgcacggcg tcccgggtcg tcacgtcgtc gctgtagagg aggaccgaca
     2761 cccggtggat gccgtcggac tccggggcct gggccgaggt gtcggtgccg tgcgatgagg
     2821 tcatgcgcgc atcgtaccgg tggcgacagc gacccgccgg gaaggcctgc gtggtggcca
     2881 gaacacctcg tcgccgctgc cgttcagcgg aaactttcgc ggacttgtcg agcgttttgg
     2941 ggggtccccg cagcacgcga gagaacttcc ggccataatg gcgttcgtgg cgactcagac
     3001 agcttcccag accaggcccg cggggcccgt gccttcgatc cccggccagg ggccagcgac
     3061 ccgaccgaac ctggtcgcgg tcggcaccat ggtgtggttg tcgagcgagt tgatgttctt
     3121 cgcggcgctc ttcgcgttct acttcacgat ccgcggccac gagccgcagc tgtgggccga
     3181 gaacacggcg atcctcaaca tcccgttcgc cctcgccaac acgatcgtcc tggtcatctc
     3241 ctcggtgtgg tgccagctcg gtgtgctgca ggccgagaag ggcgtcgtcg cacggccggc
     3301 cggcaccaag ctgaccaagg tctccgcgtg gggcatgcgt gagtggtacg tcctcaccta
     3361 catcttcggt gcggtcttca tcgccggtca ggtctacgag tacgcccacc tcgtcgccga
     3421 gaacgtcacg ttgtcctccg acgcctacgg ctcggtcttc tacctcgcga ccggcttcca
     3481 cggcatgcac gtcaccggcg gcctcatcgc cttcctgctc atcatcgggc gcaccttcac
     3541 gacccgccgc tacacgcacg cgcaggccac cggcgcggtc gtcacgtcgt actactggca
     3601 cttcgtcgac gtcgtgtgga tcgccctgtt catcatgatc tacgtgctga agtgacccag
     3661 acgatgacgc accccacgac cacgaccccg cagacgacga caggaacgca cccgtgaact
     3721 cactggcagc ccgccgccgc cacccagccg cgatcgccgt cctgatcatc ttgggcctct
     3781 tcctcacggg cgccgtgtac gcggcgttcg cgcccaagcc ggctgacgcg accacgaagg
     3841 ccctcccggc acagacggtc gaagagggca aggccctgtt ccgggccaac tgcgcctcct
     3901 gccacggcct gaacgccgaa ggccgcgcca ccggcaacgg cgacaaggtc gcgggccccg
     3961 cgctcgccgg cgtcggtgcc ggtgccgtcg agttccagat gggaacgggc cggatgccgt
     4021 tggccgggcc gaaggtgcag gctcccgcgc gcggcgaggt gaagttctcg caggaccaga
     4081 tcgacgcgat cggggcctac atcgcctcgc tgtcccccgg ccccgagcgg ccgagcgccg
     4141 aggccatcga ccccaccaag ggtgacccgg ccaagggtgg cgagctgttc cgcgtgaact
     4201 gcgcgatgtg ccacaacttc gccggtgccg gcggtgccct gacccgcggc aagtacgccc
     4261 cggccctcac cggtacgagc gacgagcaca tctacctggc catgaccacc ggcccccaga
     4321 gcatgccggt gttcaacgac tccaacctct cccccgaggc caagcgcgac atcatcgcgt
     4381 tcctcaacac catcgaggag caacccaagc agggtggcct ctcgctcggc tcgatgggtc
     4441 cggtctccga gggcctgttc gcctgggtct tcggtctggg catcttcgtg gcctgtgccg
     4501 tgtggctcgg ctccaaatcg gcctagcacc cctagtcgtt tccctacccg cccgtcgaac
     4561 agaagatcgg taccccgatg aacgagcagg aaacccacca ccccggtgct gaggtggagc
     4621 ccgcctccac ctccagcacg gccgtgaggc ttgatgaggg ccacatcatc ggcacgggcg
     4681 gcgtccccga gcgcttcacc aaccccgggc tgcccgccca cgtcgcccga gctgcggacc
     4741 tcgacgaggc tgcggcccgt cgcgcggaga agcaggtggc gaccctgttc ggcctgtcca
     4801 tcctcggcac gctggcgttc atcgtcctgt acttcgcggt cgacctcgac cagacggtct
     4861 tcgtcccggg gatcggcgtc accaacgcct ccaacctcgc tctcggcacc acgatggcgc
     4921 tcggcatgct cggcatcggg ttcggtgccg tccactgggc caagaccctc atgcccgacg
     4981 aggaggtcgt cgaggagcgc cacccgatgc gcgcatccga cgagtcccgc gagcgggtcg
     5041 tgaaggtgct cgccgagggt ggcgagcagt cccagatcac ccgccgcccc ctgatcaagt
     5101 acacgatggg tggggcgctc ggtctgttcg ccgtgccact cgtgctccag gtggccggca
     5161 gcctcggccc gatgccgcag aagagcctgt cgcgcacctt ctggaacggc ggccccagcg
     5221 gcccgggtga ggaccccgag ttccgcccgc ttcgcctgat gcgcgacccg gagggcacgc
     5281 cggtcaaggc cgaggacgtc accatcggct cggtcttcca catcatgccc gagggcctgc
     5341 tccccaaccc ggacgacccg gaggcggagt acctcgagaa gcacatcctg gaggagaagg
     5401 ccaaggccgc cgtcatcctc atccggctcg acgaagacct catcgagagc cagaagcagc
     5461 gcgactgggg ccaccagggc atcgtcgcct actccaagat ctgcacgcac gtcggctgcc
     5521 ccgtcgggct gtacgagcag cagacccacc acctgctgtg cccgtgccac cagtcgacct
     5581 tcgacgtgac gcaggactgc aaggtcatct tcggccccgc caagcggccg ctgccacaac
     5641 tgaagatcac cgtcgacgac gagggctacc tcgtctccgc cgcaccgttc gccgaagcgg
     5701 tcggcccgag cttctgggag cgtggctgat gagcaccacc accgcccgac ccgccgacgc
     5761 cctgcgcgag ggggacaccc ccgtcgaggc cgctccctcc ggagccgccc gtcgcgtcgg
     5821 tggcatcgcc ggctgggtcg acgaccgcac cggggccgcc aagccggtcg gctacctcat
     5881 gaagaaggtc ttccccgacc actggtcgtt catgctcggc gagatcgcga tgtactcgat
     5941 gatcatctgc ctgatcaccg gtgcgttcct caccttctgg ttcgtgccga gcgccggtca
     6001 cacggtctac gacggctcct tcgtgccgct tcgcggtgtc gggatgtccg aggcctacgc
     6061 ctcgacgctc aacatctcgt tcgacatcaa gggcggcctg atcatccgcc agatccacca
     6121 ctgggccgcg ctgatgttca tcgtcgccct cacggtgcac atgttccggg tcttcttcac
     6181 cggtgccttc cgcaagccgc gcgagatcaa ctgggtgatc ggcaccgtcc tggcgctgct
     6241 ggccatcgtg gagggcttcg ccggctactc cctccccgac gacctgctct cgggtaccgg
     6301 catccgggcc atggccgggt tcgtgcagac cgccccggtg atcggcacct acctcgtgta
     6361 cggcatcttc ggcggcccgt tccccggcga gatgatcatc ccgcggctgt acagcgtgca
     6421 catcctgctg ctcccggcga tcctgatcgg gctgttcacg gcccacatcg gtctggtctt
     6481 cgtccagaag cacacccagt acccgggccc gggccgcacg aacgagaacg tcgtcggttt
     6541 cccggtcatg ccggtgtacg cggcgaaggc cggtggcttc ttcttcatcg tcttcggtgt
     6601 cctggccatg atctcggcac tggtgcagat caacccggtc tgggcctacg gtccgtacga
     6661 cccctccccc gtcacggccg gctcccagcc cgactggtac atgggcttcg ccgatggtgc
     6721 gctgcggctc ctgccgggat ggctggagtt cgagttctgg ggctacaccc tgtcgctcaa
     6781 catcgcgctc ggtgccctgg tgttgctccc gctcgtctac acggtgctcg gcatctaccc
     6841 gttcgtcgaa cgctgggtca ccaaggacga ccgtgagcac cacctgctcg accgcccgcg
     6901 caacaacccc gtgcgcaccg gcatcggcat ggcgggcatc accgcctact cggtcttcat
     6961 gttcgccgcg ggcaacgaca tcatggcgat caagctgggc atgtcgatca acgacatcac
     7021 ttggttcttc cgcatcgggc tgttcgtcct gccgccgctg atgttctggg tcaccaagcg
     7081 catctgcctg tcgctgcagc gtcgcgaccg cgacaccgtc ctgcacggcc gggagacggg
     7141 caccatcatc cgcaccccgg acggcaagta cttcgagcgt cacgacaccg agctcaccgc
     7201 cgaccagtgg gtgctggtgc agcacgaccc ggtggctccc ctcgagctcg agaccgaggt
     7261 cgacgagcac ggtgtggccc gcaagaccag tcgactggcc aaggccaagg tggcgctctc
     7321 gaggttctac ttcgcggatg ccgtcaaccc tgtcaccccc gcggaactcg ccgctgctca
     7381 ccaccacggt gaggagcacg aggccatcga ggccgccccg tccgccgacg agtccgagcc
     7441 tgcgcaggtc ggtcgccact gacctgctcg tcagctcccg acacggggcg caccgccagc
     7501 cgatcacggc tgaggggtgc gccccgttcg cataggaccc gatacggagc cggcagggcc
     7561 tctgctgctg ggcctgcaag gcgtccgcca ctgggcctgc aaggcctccg ccactgggtc
     7621 tgcacggcct cgagcgctac cgggccagca gagcctcgag ggtgcgacgc ccttggtcgg
     7681 catcaacgca cacctctagc accttggtgc gtgagcgctc ccccgccacc aggctgacct
     7741 cccggggtcg cacgccgaac gcgcctgcga cggctgcgca cacggccgcg gtggcggcac
     7801 cgtccaccgc ccgggcactc acggacaccc cgagcacctc accgtcgccc caccggccac
     7861 ccacccgcgt gcgtgacgct cctgggcgga ctcggacggt gacgtgcacg aagcccatcc
     7921 tgccagcgga ccgacagcca ccagcgctcg gtagggtggg cgcagtgagc cacgaagaca
     7981 ccggggagtt gccgcggagc acacccgaga gccaaggagt cccctcgggt gccgtcctgg
     8041 cgatgctgcg ccgctgggag accagcgggt gcgaaccgca cagcgtggtg atcgtgcgtc
     8101 atggccatgt cgtcgcgcag ggctggtggg cgccctaccg accagagcac cagcagctgc
     8161 tctactccgt gagcaagacc ttcctggccg tcgccgtggc actggccgag gacgacgggc
     8221 tgctcgggcg cggtgagcgg ctcgtcgacg tcttccccga ggcggcagcc acggccgggc
     8281 cgcgggcctc ggagatcacc gtcgaggact gtctgcggat gtcgaccggg caccacgagg
     8341 acaccctgga caccgtgcca ggcctcctgg ggtcgggtga tggcgctgcc gccctgttcc
     8401 tggccgcgga gcccgagcac gagccggggt catggttcgt ctaccacaac ggcgcgagcc
     8461 tcatgctggc cctggcggtg cagcgccgca ccggccagcg actcctgcac tacctgcgcc
     8521 cccgcctgct ggagcccctc ggcatcacct cggcgagctg gcgcagcgcc gcggggggcg
     8581 acctcggctt cagcggcctg cacaccacga cggacgctgt ggcccggctc ggcgtgctgc
     8641 tcctcgatga cggcgtgtgg cagggcaccc ggctcctccc gagcgggtgg gtgggcacag
     8701 cgagcagcgc cctgagcaac acggcacacc acccggggac gcccgactgg cagcagggct
     8761 acggctacca gctgtggcga tctcgccacg acggctttcg agccgacggg gcctatgggc
     8821 agttcagcct catccaccct cggcacgacc tcgtcgtcgc cgtgacggcc tgcaccgacg
     8881 acgcacagcc gatcctggag gccgtctggg aagaactgat cccggctctc accgacacac
     8941 cctccccagc cgaccccgag gccgttcacg agctctcgca ggcactggac accgcggtgc
     9001 ttcccgctgt ggggtccacg tgcgagcccg cagtccctgg cccgtgggtg ttcacgcacg
     9061 aaccgacccc ggagcacccg cgtctcacgc gcgtcgaggt gcgcccgcac gaaaacagct
     9121 ggctgctcgt cgtcgatgac ggcgagccac tgccgatcac ctgcggcgac gggcggtggc
     9181 cggatgccgc tggcgcgcct ttcgtcgccg ccggcggctg ggtgtcgcca gacgtcttcg
     9241 aggccacgat cgtcgccgtg cagactcccc actcgctgca cctgcactgc accgacggcg
     9301 tcgcctccgc gcggtggcgc gggtacccct tgcacgggcc ctgcctgtcg tggcagcgcg
     9361 aaccggatgg agactgacat gcgccccgtc acccacgacg agttcgacgc gatcgtgcag
     9421 gacgcgctcg acgacgtccc tgccgagctg atggccatgc tcgacaacgt cgtcttcttc
     9481 gtcgaggacg agccctccgc ggacgacccc gatctcctgg gcatctacga gggcaccccc
     9541 ctcaccgagc gcggcgagtg gtggggtcag ggatcactcc cggaccggat caccctgttc
     9601 cgcggcccgc tgaccaggat gtgtgcggac gccgaggaac tgcgtgagga gatcgccgtc
     9661 accgtggttc acgagatcgc ccaccacttc ggcatcgagg aagaaaccct gcacgacctc
     9721 ggctggggct gagagacgcg ccgcgcccgg gctcagagcg cttccgacac acctcggccc
     9781 caccggagac tgctccggtg gggccgatgg gtgcgaggtg agagcgctca aactctctcg
     9841 agcggcatcc tcactcgtgc ggcgtcactc gcctcagagg gcgtgctcgc ccttccagta
     9901 ctcgaaggtc cacccgatga gggcaggcac cgcgaagaac gcgccgatga tgaagaccca
     9961 ccacccgacg gccagcccga ggaagcagac ggcggcagcc gcagccagga agagcggcca
    10021 ccagctgtgc gggctgaaga agccgtagtc gccctgaatc tcgtcgatgt cgccgagcgg
    10081 gtcgtccgac gggtcgacct cgagcttgcg ccgggtcatg ccgaggtacc acgtgatcat
    10141 gaagccgagc agcccaccga ggatcaggcc gatgacgccg acccactcga tcccaagttc
    10201 ggtggagttg gtccagaagc cgtagatcgt cgccatgagg aaggcgaaga ctccgagcag
    10261 ggcgccgatg cgctccatcg cgagcatggg gctcagccct ccttcttctc gtcggcgaag
    10321 gcattgcctc cgaggtcagc cggaccgagt gccgagacca gcacgttgtc gtcggcgacc
    10381 gcggcgtcgt gcacaccggc gggcgctgcc tcagggtggt gcaggtcgaa cgcgggacgc
    10441 tcggagcgga tgcgcgggat gctgttgaag ttgtgccgcg ggggcgggca ggaggtggcc
    10501 cactccagcg aggcgccgta gccccacggg tcgtcgacct cgaccagtgg cgcggtcttc
    10561 cacgtcttcc acacgttgta catgaacggc agcatcgaca ccgcgaggac gaacgacccg
    10621 accgtcgaga tctggttgcc cagggtgaag ccgtcctcgg gcatgtagtc ggcgtagcga
    10681 cggggcatgc cctggatgcc gatgaggtgc tggacgagga acgtggtgtg gaagccgatg
    10741 aacagcagcc agaagtggat cttgcccagg cgctcgtcga gcatgcggcc ggtgagcttg
    10801 ggccaccaga agtagaagcc ggcgaacatc gcgaagacga cggtgccgaa cacgacgtag
    10861 tggaagtggg cca
//
LOCUS       DAOSOC010000078        10562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_6286_length_10562_cov_8.595317, whole genome shotgun sequence.
ACCESSION   DAOSOC010000078 DAOSOC010000000
VERSION     DAOSOC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6286_length_10562_cov_8.595317"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PL014_07390"
     CDS             <1..784
                     /locus_tag="PL014_07390"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOT56949.1"
                     /translation="VTETNLVINRRSGEVDRRRTTSMNHLVTRTAKDPVQTEVIAKWK
                     ALSGELGAQVVGTHVEDIIGHSDPRSNRAIETPMGNLVADAILWGTSGDNGGAQIAFM
                     NVGGVRASLPMAPVAGEGVGNITYAEAYAVAPFGNLLNTITMTGAQIKAVLEQQYRVS
                     GAGVASTLSINVSEGFTYTWTAGNAAGSKVSDMRLNGVPLSMDQTYRVGTLSFLAQGG
                     DGFSVFTQGTNLTGGPEDLKNLVDYFRAHPGLTAPGDRVAGL"
     gene            874..2997
                     /locus_tag="PL014_07395"
     CDS             874..2997
                     /locus_tag="PL014_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT56950.1"
                     /translation="MPSTTPPVPPRRPHVREHHGDVVHDPYAWMADLEDPELLAHLEA
                     ENAYADARTTHLEGLRGELFQEIRSRVKETDLSVPVASGPWWYFSRTVQGRQYASHVR
                     APLTDPAVRPDPEADAMAGEQVVVDGNVEAGDSEFFSLGAMTVSADHRLVAFAVDTAG
                     DERFDLVIRDIGTGEVLDRSVTGIGYGVELSLDGTFVFYTRLDEAWRPHQLWRHRVGG
                     DPADDVLVHQEDDERFWMGIGSSRDERWLMLGLGSKTTSEVHLLDSADPDGEWRVVAP
                     RREGIEYDVEPAGDRLLIVHNTDTPDADLAWAPIDATSHEQWVPLLTSTDGERFVSVD
                     AFDTASVLSLRSGGLTALRVLPRDATSDSGHAAGWDVTVDAPVHSIGLGDNPEPGQTS
                     VQVVVESWATPRSILDVDLHTGERTLLKRQPVLGDFDPANYAERREWATASDGTLVPV
                     SVVHRVGVEPDGTNPGLVTAYGAYEISSDPYFSVARLSLLDRGVVYAVAHVRGGGELG
                     RRWYDEGKLLAKTNSFTDTLACVDHLVATGWIDPERVGLEGGSAGGLLVGAVLNLAPE
                     TFRVAHAAVPFVDALTTMLQPDLPLTIGEWEEWGNPLTDPEVYAAMRAYTPYENVRPA
                     RYPAVLATSSLHDTRVYVTEPAKWVTRLREVATNDLEQRPVLMRTELAAGHGGRSGRY
                     AAWEQIAWEWAFVLDQLGATRRLSG"
     gene            complement(3008..3871)
                     /locus_tag="PL014_07400"
     CDS             complement(3008..3871)
                     /locus_tag="PL014_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT56951.1"
                     /translation="MSASRVVVGVDGSPLSMAAVSAAAQIASERGLPLHVLHAFAVDL
                     PMLGFGELTKDSDVVSTHASRLVAQGVARAHAIDPSLTVTTAVRDGYASQALVDAART
                     AALVVVGAMGHGLFSRASVGAVAMQVVTHARCPVLVVGHEGTPPASESAAVVVGVDGS
                     KASLRALSAAFDEAVRRSAPLRVVHAWEPSSASDPTLSGDSTWSDYAADLERTLSSAL
                     SAQQASNPHVEVSYEVVTGEPVQTLLDHARDAGLLVVGSRGSGGFPGLHVGSTALRLM
                     GRSPCPVLFTR"
     gene            complement(4003..5859)
                     /locus_tag="PL014_07405"
     CDS             complement(4003..5859)
                     /locus_tag="PL014_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HOT56952.1"
                     /translation="MSPRPTLRQTSPSPRTRRARSAGALTLALAMIPAAASAAHGDDP
                     TVLTPIGGGYVTSTLAGFADAAALGASGPTVDLVVVPAAYGDGAKDRAENLRLAADRT
                     AQLDAACDAAVASSGSGFTGCTATLAVLLNRADALDPANAVALADPGTDGIYVLGGDQ
                     GLAMQVLADSPAEAAITAAVEGGAALGGTSAGAAVESRTMINGYRGSLGPADGLQQGS
                     TLIWWGDDGDLERGLDIGSSRAIFDQHFHQRGRLGRSLSTIATAGERLRTSPVGVGVD
                     YATGVRVTGDTVLSDVFGDGAVTIIDDQTLGGTHSWVGSPATLSARRVLTHLMTEDTT
                     YDLASREFTRAGEVIAPPPASAWLAPASPSTVEGTVFLGGGSLGSPVIADVVAAARTV
                     DASKKARLVVLGGGASSSSLTSAYAQAVKKAGWAGQVNAVVHNGKGWPGTSLAGATAV
                     VLVGEDPVALASSMADPAFRATVTQAVRTTPVVFADGSMTAVLGERWSAKARPTSNTL
                     EAEGVAAYRADDAAWQDGLALVPATVVPHLTDDFRWGRLYAGVAAAPADLAIGVASGS
                     AVVLSPAGASVSGASVVVADGSGASSWVSANGAFGASGVVLDVFGSGDQLAR"
     assembly_gap    5880..5979
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6000..7043
                     /gene="mtnA"
                     /locus_tag="PL014_07410"
     CDS             6000..7043
                     /gene="mtnA"
                     /locus_tag="PL014_07410"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOT56953.1"
                     /translation="MTLIAISWDDAAGRDGRGAVRLIDQTLLPGTVVDRVTDTTDDLV
                     EAIAVLAVRGAPALGVAGAMGVVVAMDEGAREGWDEARVQAAVDRVREARPTAVNLAW
                     GVDQVRPLMGNGRDAVLAAARQLAAEDEAANRELSRLGADWLLSRIDRPRLRVLTHCN
                     TGSLATSAWGTALGVIRELHARDRIELVYADETRPLLQGARLTAFELEQDGIPHVVQV
                     DGAAASTILRGLVDFAVVGADRIAANGDAANKIGTLGVALACRDADIPFMVAAPWSTV
                     DLSMADGSGIEIEERSGEEITSIGGVRVAPAGSGGFNPAFDVTPARLVDAVATERGVA
                     QPRSGQNLWSIAP"
     gene            7102..7758
                     /locus_tag="PL014_07415"
     CDS             7102..7758
                     /locus_tag="PL014_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HOT56954.1"
                     /translation="MDSMKNNASRCTLIGDIVGSRGSVDRADLHRAVEAALRQVDDAV
                     PSLTGLRVTVGDEFQGSYATLGEALDAGLRVRLALLPDVDTRVGIGRGSVTTLDPDRG
                     IEDGPGWWAARSAVEAAEEAAAKPATRHVRTALRVAPSEDDTAVDAVNAALLCRDHLV
                     GSCSERSIRLLRGLMQPHTTQGDLATLEGISPSAVSQRVRADGLGAVLAAHDLLRRMP
                     "
     gene            7755..8525
                     /locus_tag="PL014_07420"
     CDS             7755..8525
                     /locus_tag="PL014_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56955.1"
                     /translation="MSWLGVWLVGLGLADLVRAAAPPPVARWSPAVGAAVIAALAALA
                     GLTAPTDLVVLGVSMVALALWAVLSAQALSTGRGHGRALAVLGAVAFALVALSGWASS
                     AGGLLASWLAWADLPLLDAASDPGAVLLVTGLLLANLATANVVVRLVLVSVGALRPVQ
                     QAELPSGPQASDQLRGGRLLGPMERVLIIGLGLAGQLTAAGLVIAAKGLIRFPELQAK
                     RDESSTVDAVGIDEVTEYFLVGSFVSWLVALASLALTR"
     gene            complement(8573..9202)
                     /locus_tag="PL014_07425"
     CDS             complement(8573..9202)
                     /locus_tag="PL014_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOT56956.1"
                     /translation="MTTERTTLIDQLLDAGRHLVTRGLVQASGGNLSVRLPGTDHFLV
                     TGSGTWLDRLTADDLVELTLHGERVSGAQRPSVEWKLHQRTYAVRPDVQAVIHLHPQH
                     VLLVDMLGAPIRFTTLDHQFYLGSAGRVPFHPAGSQEIADAAADAARDHDAVVMAHHG
                     CSTLGDTVSMALRRALNLEEAAAMTYRLLMAGDTTTDFPQEWKGNIISI"
     gene            complement(9327..9776)
                     /locus_tag="PL014_07430"
     CDS             complement(9327..9776)
                     /locus_tag="PL014_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT56957.1"
                     /translation="MATTFDPFRDLDRVLSGSLRTPASAAMPMDLYRSGDHFVAHIDL
                     PGVDPSTIDVDVEERTLTIRAERRDTTDEDVQWLSHERPTGTFARQLTLGYGVAPDRI
                     EADYSDGVLTLTIPVADEAKPRKISVSHKGSRQVIEGEASAAQPAGA"
     gene            complement(9906..10373)
                     /locus_tag="PL014_07435"
     CDS             complement(9906..10373)
                     /locus_tag="PL014_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56958.1"
                     /translation="MGSNPRRLDGLRPDERAAALLLEHVTGGTVRAHDVGTRQGAYDL
                     HLTYPKGRTAAVEVTTANSLPADPFSLLVLPHVARRLVKVVSAGGVDERHLFIGITSE
                     PSAEAEWPALAAQDVAPYDDVPEGLTRLWIATTGRAPGLTSWSRPDGWQHHTR"
BASE COUNT         1446 a         3652 c         3840 g         1524 t
ORIGIN      
        1 cgtcaccgag accaacctcg tcatcaacag gcgcagcggt gaggtcgacc gtcgccgcac
       61 cacgtcgatg aaccacctcg tgacccgcac ggccaaggac cccgtgcaga ccgaggtcat
      121 cgcgaagtgg aaggccctca gcggagagct cggcgcgcag gtcgtcggca cccacgtcga
      181 ggacatcatc ggtcactccg acccccgctc gaaccgcgcc atcgagaccc ccatgggcaa
      241 cctcgtcgcc gacgcgatcc tctggggaac ctcgggggac aacggcggag cgcagatcgc
      301 gttcatgaac gtcgggggcg tgcgcgcctc actcccgatg gcgccggtcg ccggtgaggg
      361 tgtcggcaac atcacctacg ccgaggccta cgccgttgct ccgttcggca acctgctcaa
      421 caccatcacg atgaccgggg cccagatcaa ggcggtcctg gagcagcagt accgtgtcag
      481 tggcgccggt gtcgcgagca cgctgtcgat caacgtctcc gagggcttca cctacacctg
      541 gacggcgggt aacgccgccg gctccaaggt cagcgacatg cgcctcaacg gtgtgccgct
      601 gagcatggac cagacctacc gcgtgggcac gctgagcttc ctcgcgcagg gcggtgacgg
      661 gttcagcgtc ttcacccagg gcaccaacct cacgggtggt cccgaggacc tgaagaacct
      721 cgtggactac ttcagggccc acccgggcct gacggctccg ggagaccgcg tcgccggcct
      781 gtgaccgacc ggtccctgag cacagcagcg agggccgcac tcccgaccgg ggtgcggccc
      841 ttgccgcgtg gtggcccggc atagggtgac gccatgccca gcaccacccc accggtcccg
      901 ccgcgccggc cccacgtccg cgagcaccac ggcgacgtgg tccacgaccc ctacgcctgg
      961 atggccgacc tcgaggaccc cgagctgctc gcccacctcg aggccgagaa cgcctacgcc
     1021 gacgctcgca cgacccacct ggagggcctg cgcggtgagc tcttccagga gatccggtcg
     1081 cgggtcaagg agaccgacct gtccgtgccg gtcgcgagcg gcccgtggtg gtacttctcg
     1141 cgcaccgtcc agggccggca gtacgcctcc cacgtgcggg cccccctcac cgacccggcc
     1201 gtgcgtcccg accccgaggc ggatgccatg gcgggcgaac aggtcgtcgt cgacggcaac
     1261 gtcgaggccg gcgacagtga gttcttctcc ctcggggcga tgaccgtcag cgccgaccac
     1321 cggctcgtcg cgttcgcggt cgacaccgca ggcgacgagc gtttcgacct ggtcatccgc
     1381 gacatcggga cgggtgaggt gctcgacagg tcggtcacgg ggatcggcta cggcgtcgag
     1441 ctgagcctgg acggcacctt cgtgttctac acgcgtctcg acgaggcctg gcgcccgcac
     1501 cagctgtggc ggcaccgcgt cggcggtgac ccggccgacg acgtgctcgt gcaccaggag
     1561 gatgacgagc ggttctggat gggcatcggg tcaagtcgcg acgagcgctg gctcatgctg
     1621 ggcctggggt ccaagacgac gtccgaggtg cacctgctgg actcggcgga cccggatggg
     1681 gagtggcgcg tcgtcgcgcc ccgccgcgag ggcatcgagt acgacgtcga gccggccggt
     1741 gaccggctgc tcatcgtcca caacaccgac accccggacg cagacctcgc ctgggcgccg
     1801 atcgacgcga cgagccacga gcagtgggtt ccgctcctga cctcgaccga cggcgagcgg
     1861 ttcgtctccg tcgacgcctt cgacacggca tccgtgctct ccctgcgcag cggcggcctg
     1921 accgcactgc gggtgctgcc ccgcgacgcg acgagcgact cggggcacgc cgccggctgg
     1981 gacgtcaccg tcgacgcccc cgtgcactcg atcgggctcg gcgacaaccc cgagcccggg
     2041 cagacgtcgg tccaggtcgt cgtcgagtcg tgggcgacac cccgctcgat cctcgacgtc
     2101 gacctgcaca ccggggagcg caccctcctc aaacgccagc cggtgctcgg ggacttcgac
     2161 cccgcgaact acgccgaacg acgcgagtgg gcaacggcat ccgacggcac gctcgtgccg
     2221 gtctcggtgg tgcaccgcgt gggcgtcgag cccgacggca ccaacccggg gctcgtcacg
     2281 gcgtacgggg cgtacgagat ctcctccgat ccctacttct ccgtcgcccg tctcagcctg
     2341 ctcgaccgcg gtgtcgtgta tgccgtggcg cacgtccgcg ggggcggcga gctcggccgc
     2401 cgctggtacg acgagggcaa gctgctcgcc aagaccaact cgttcaccga caccctcgcc
     2461 tgcgtcgacc acctcgtggc gaccggttgg atcgaccccg agcgcgtcgg gctcgagggc
     2521 ggctctgccg gtgggttgct cgtcggggcc gtgctcaacc tcgcgcccga gaccttccgc
     2581 gtcgcgcacg ccgcggtgcc gttcgtcgac gcgctgacga cgatgctcca gcccgacctg
     2641 ccgctgacga tcggggagtg ggaggagtgg ggcaacccgc tcaccgaccc cgaggtctat
     2701 gccgcgatgc gggcctacac cccctacgag aacgtgcgcc cggcccggta cccggcggtg
     2761 ctcgcgacgt cgagcctgca cgacacccgc gtctacgtga ccgaaccggc caagtgggtc
     2821 acgcggctgc gtgaggtcgc gaccaacgac ctcgagcagc ggcccgtgct gatgcgcacc
     2881 gaactcgccg ccggacacgg tggccgcagc ggtcggtatg ccgcgtggga acagatcgcg
     2941 tgggagtggg ctttcgtgct cgaccagctc ggcgcgacca ggcgcctcag cgggtgaaca
     3001 acacgcctca gcgggtgaac agcaccgggc aggggctgcg gcccatgagg cgcagcgccg
     3061 tcgacccgac gtgcagcccg gggaagccgc ccgaaccacg gctgcccacg acgagcagcc
     3121 cggcgtctcg cgcgtggtcg agcagggtct gaaccggctc acccgtgacc acctcgtagc
     3181 tgacctcgac gtgcgggttg gaggcctgct gtgcggacag tgctgagctg agggtgcgct
     3241 cgaggtcggc ggcgtagtcg ctccacgtcg agtcgccgga caacgtgggg tcgctggcgc
     3301 tgctgggctc ccaggcgtgg acgacgcgca ggggagcgct gcggcggacg gcctcgtcga
     3361 acgcggcgga cagggctcgc agggaggctt tggacccgtc gacgccgacg acgaccgcag
     3421 cactctcgga tgccggcggg gtgccttcgt ggcccacgac gaggacgggg caacgggcgt
     3481 gggtcacgac ctgcatcgcg acggcgccga cgctggcccg gctgaacagc ccgtgcccca
     3541 tggcgccgac gacgacgagg gctgcggtgc gtgcggcgtc gacgagggcc tggctcgcgt
     3601 agccgtcgcg aacggcggtg gtcaccgtga gggaggggtc aatggcgtgg gcgcgggcaa
     3661 ccccttgggc gacgagcctg gacgcgtggg tggacacgac gtcggagtcc ttcgtgagct
     3721 caccgaaccc cagcatcggg aggtcgacgg cgaacgcgtg cagcacgtgc aggggcagcc
     3781 cccgctcgct cgcgatctgt gcggccgcgg acactgccgc catggacagg ggggatccgt
     3841 cgaccccgac gacaactcgt gatgcgctca tccaggtttc ctccgatcgc gagtgtttcg
     3901 cggcggcggg ctgaggaagt ccgtcgtcgt ggaaccaagg gctccacacc tcgaccgtgt
     3961 cacggtgcgc gccggcacgc aaggacgcgg gtcacatcat gttcagcggg cgagctggtc
     4021 accggacccg aagacgtcga ggacgacccc gctcgcgccg aacgcgccgt tggcgctgac
     4081 ccacgacgac gcccccgacc cgtcggccac gacgaccgac gcacccgaga ccgaggcgcc
     4141 tgcagggctc agcaccaccg ccgacccgga cgccaccccg atggcaaggt ccgcgggtgc
     4201 cgccgcgaca cccgcgtaca gccgacccca gcggaagtcg tcggtgaggt gggggaccac
     4261 ggtggcgggc accagcgcga gcccgtcctg ccacgccgcg tcgtcggcgc ggtacgccgc
     4321 gacgccctcg gcctcgaggg tgttcgacgt gggtctggcc ttggccgacc agcgctcgcc
     4381 gaggacggcg gtcatcgagc cgtcggcgaa caccaccggg gtggtgcgca ccgcctgggt
     4441 gaccgtggcc cggaacgccg ggtcggccat cgacgacgcg agcgccaccg ggtcctcccc
     4501 gacgaggacg acggccgtgg cgccggcgag cgacgtgccg ggccagccct tgccgttgtg
     4561 gacgaccgcg ttcacctgcc cggcccaccc ggccttcttc acggcctggg cgtaggcgct
     4621 cgtcagcgag ctcgacgacg caccgcctcc gaggacgacg agccgggcct tcttcgacgc
     4681 gtcaaccgtc cgggccgcag cgaccacgtc ggcgatgacc gggctgccga gtgacccgcc
     4741 accgaggaac accgtcccct cgacggtcga cggggatgcc ggggcgagcc atgcgctcgc
     4801 cgggggaggg gcgatcacct cgcccgcgcg ggtgaactcg cgcgaggcca ggtcgtaggt
     4861 cgtgtcctcg gtcatcaggt gggtgagcac gcggcgggcc gacaaggtcg ccggactgcc
     4921 cacccaggag tgggttccgc cgagggtctg gtcgtcgatg atcgtcacgg caccgtcgcc
     4981 gaagacgtcc gacaggacgg tgtcaccggt gacccgcacc cccgtcgcgt agtcgacccc
     5041 caccccgacc gggctggtgc ggaggcgctc gccggcggtc gcgatcgtcg acaggctccg
     5101 gcccagacgg ccgcgctgat ggaagtgctg gtcgaagatc gccctcgacg agccgatgtc
     5161 gagaccgcgt tcgaggtccc cgtcgtcacc ccaccagatg agggtcgagc cctgctggag
     5221 accgtcagcc ggccccagcg agccgcggta cccgttgatc atcgtgcggg actccacggc
     5281 ggcgccggcg ctggtcccgc cgagggcggc gccgccctcg acggctgcgg tgatggccgc
     5341 ttcggccggg gagtcggcga gcacctgcat ggccagcccc tggtcgccgc cgaggacata
     5401 gatgccgtcg gtgccggggt cggccagggc gacggcgttg gcgggatcga gcgcgtcggc
     5461 gcggttcagc agcaccgcga gcgtggcggt gcacccggtg aagccgctgc ccgaggacgc
     5521 gacggcggcg tcgcacgcag cgtcgagctg ggcagttcgg tcggccgcga ggcggaggtt
     5581 ctcggcccgg tccttggcgc cgtcgccgta ggccgcgggg acgacgacga ggtcgacggt
     5641 gggtccgctg gcaccgagcg ccgcggcatc cgcgaacccg gccagggtcg aggtcacgta
     5701 cccgccgccg atcggcgtca gcacggtcgg gtcgtcaccg tgggcggcgg acgcggcggc
     5761 cgggatcatg gcgagcgcca gggtgagggc accggcggag cgggcccggc gcgtgcgagg
     5821 gcttggcgag gtctggcgca gggtgggtcg cgggctcatg gtcggacgct agcccctcgn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcaccctagg ctggcgccca
     6001 tgacgctcat cgccatctcc tgggacgacg ccgcgggccg cgacggtcgc ggtgccgtcc
     6061 ggctcatcga ccagaccctg ctgcccggga ccgtcgtcga tcgggtcacc gacaccacgg
     6121 acgacctcgt ggaggcgatc gccgtgctcg cggtgcgcgg cgctccggcg ctcggggtgg
     6181 ccggtgccat gggtgtcgtc gtcgcgatgg acgagggcgc ccgcgagggc tgggacgagg
     6241 cccgggtgca ggccgctgtc gaccgggtgc gcgaagcgcg gcccaccgcc gtcaacctcg
     6301 cgtggggggt cgaccaggtg cggccgctga tgggcaacgg cagggatgcc gtgttggccg
     6361 cggcccgtca gctcgccgcc gaggacgagg ccgccaaccg tgagctgtcg cggctcgggg
     6421 cggactggct gctgtcccgg atcgaccgcc cccggctgcg ggtgctgacg cactgcaaca
     6481 cgggatcgct cgcgacgtcg gcctggggca ccgccctcgg cgtgatccgc gagctgcacg
     6541 cccgcgaccg catcgagctc gtctacgccg acgagacgcg ccccctgctg cagggggccc
     6601 ggctcacggc cttcgagctg gagcaggacg gcatcccgca cgtggtccag gtcgacgggg
     6661 ccgctgcctc gacgatcctg cgggggctgg tcgacttcgc cgtcgtcggt gccgaccgca
     6721 tcgccgcgaa cggggacgcc gcgaacaaga tcggcaccct cggggtggcg ctggcctgcc
     6781 gtgacgcaga catccccttc atggtcgctg cgccgtggtc gaccgtcgac ctgtcgatgg
     6841 ctgacggctc cggcatcgag atcgaggagc gttcggggga ggagatcacg agtatcggtg
     6901 gggtccgggt ggcgcccgcg ggctccgggg gcttcaaccc ggcgttcgat gtcacgcccg
     6961 cgcgtctggt ggatgccgtg gcgaccgagc gtggggtcgc ccagccgagg tcagggcaga
     7021 acttgtggtc gatcgcccca tgaagggttc tgctctgtag ttctgggctt catttcgttc
     7081 atgtgaaggt atgatcttca tatggactca atgaagaaca atgcttcacg atgcaccctc
     7141 atcggtgaca tcgtcggctc tcggggttcc gtcgaccgcg ctgacctgca ccgtgccgtc
     7201 gaggccgcac ttcgtcaggt cgacgacgca gtcccgtccc tcacggggtt gcgggtcacc
     7261 gtcggcgacg agttccaggg gtcgtacgcg acgctcggcg aggcgctgga tgccggtctg
     7321 cgcgtgcgcc tggccctgtt gcccgacgtc gacacccgcg tcgggatcgg gcgggggagt
     7381 gtgaccacgc tcgacccgga ccgggggatc gaggacggtc cgggctggtg ggcagcccgc
     7441 tctgccgtcg aggcggcaga ggaggctgcg gcgaaacccg ctacccggca cgtgcgcacg
     7501 gccctgcggg tcgccccgtc cgaggacgac accgcggtcg acgccgtcaa tgcggcactg
     7561 ctctgtcggg accatctggt tggctcgtgc tcagagcgtt cgatccgact gctgaggggg
     7621 ctcatgcaac cgcacaccac ccagggtgac ctggcgacac tcgagggcat cagcccgtcg
     7681 gcggtctccc agcgcgtgcg cgctgacggc ctcggggccg tgttggcggc ccatgacctg
     7741 ctgcggcgga tgccgtgagc tggctcggcg tctggctcgt cggcctcggg ctcgccgacc
     7801 tcgtgcgggc cgcagcgccg ccgccggtgg cgcggtggtc acccgctgtc ggggcagcgg
     7861 tgatagcggc gctcgccgcc ttggccgggc tcaccgctcc caccgacctc gtcgtgctgg
     7921 gggtgtcgat ggtggcgctt gccctgtggg cagtgctgtc cgcccaggcg ttgtcgacgg
     7981 ggcggggcca cggccgtgcc ctggccgtcc tcggtgccgt ggccttcgcg ctcgtcgcgt
     8041 tgtcggggtg ggcctcgtcg gccggcgggc tgctcgcgtc gtggctcgcc tgggccgacc
     8101 tgcccctgct cgatgccgcc agtgacccgg gtgcggtgct gctcgtgacg ggcctgctgc
     8161 tcgcgaacct cgccaccgcg aacgtcgtcg tccgactcgt cctggtcagc gtcggtgccc
     8221 tgcggccggt gcagcaggcc gagctaccga gcgggccgca agcctcggac cagttgcgcg
     8281 gtggtcggct cctcggcccg atggagcggg tcctgatcat cggtctcggg ttggccggtc
     8341 agctcacggc ggccgggctc gtcatcgccg cgaagggtct gatccgcttc cccgagctgc
     8401 aggccaagcg cgacgagagc tcgacggtgg atgccgtcgg catcgacgag gtcaccgagt
     8461 acttcctcgt cggttcgttc gtctcctggc tggtggccct ggccagtctg gctctgaccc
     8521 gctgagcgac ttgatctgct gagggtcctg acccggcgca gggccatcgt gctcagatgc
     8581 tgatgatgtt gcccttccac tcctgcggga agtccgtggt cgtgtcgccg gccatgagca
     8641 ggcggtaggt catcgccgcg gcttcctcga ggttcagggc ccgacgcagt gccatcgaca
     8701 cggtgtcgcc gagcgtcgag cacccgtggt gcgccatgac caccgcgtcg tggtcacggg
     8761 cggcatccgc ggcggcatcc gcgatctcct gcgaaccggc ggggtggaac ggcacccgcc
     8821 cggcgctgcc caggtagaac tggtggtcca acgtcgtgaa ccggatcggt gcgccgagca
     8881 tgtcgacgag cagcacgtgc tgcgggtgca ggtggatgac ggcctgcacg tccgggcgga
     8941 cggcgtaggt gcgctggtgc agcttccact ccaccgacgg ccgctgcgcg ccgctgacac
     9001 gctcgccatg gagggtcagc tcgacgaggt cgtcagcggt gaggcggtcg agccacgtgc
     9061 ccgacccggt gacgaggaag tggtcggtgc ccggcaggcg gaccgacagg ttgccgccgc
     9121 tcgcctggac gaggccgcgg gtgacgaggt gccgcccggc atccagcaac tggtcgatca
     9181 gggtggtgcg ctcagtcgtc atgcgaccga ttctcgccta cccacccacc ccttcccgac
     9241 cttccccacg agacgtggac gccacgtcgc cggagctcgt cgcgacggct tctcatgggg
     9301 aaggtcagcc ggggtcaggc gcggaatcag gcacccgcgg gctgggcggc gctcgcctcg
     9361 ccctcgatga cctggcggct tcccttgtgg ctcaccgaga tcttgcgggg cttcgcctcg
     9421 tcggcgaccg gaatcgtcag ggtgagcacc ccgtcggagt agtcggcctc gatgcggtcc
     9481 ggggccaccc cgtagcccag cgtgagctgg cgtgcgaagg tgccggtcgg tcgctcgtgg
     9541 gagagccact ggacgtcttc gtcggtggtg tcgcgccgct cggcgcggat ggtgagggtg
     9601 cgttcctcga catcgacgtc gatcgtcgac gggtcaaccc ccggcaggtc gatgtgggcg
     9661 acgaagtggt cgccggaccg gtagaggtcc atcggcatgg cggctgacgc gggagttcgc
     9721 agtgagcccg agagcacgcg gtccaggtcg cggaaggggt caaaggtggt ggccatggtg
     9781 tcctccttgc tcgttgggcg gccagcccaa ggttgagtgt tgatgactca atgttacgac
     9841 taaggattgg ctcaatgtca actccttggg gcgtctcccc agggcattcg ggtggcagtt
     9901 tgacgtcacc tcgtgtggtg ctgccacccg tccggacgtg accacgacgt aagccctggc
     9961 gcccggccgg tggtcgcgat ccacaggcgg gtgagtccct cggggacgtc gtcgtagggg
    10021 gcgacgtcct gagcggcgag ggcgggccat tcggcctccg cggacggttc ggaggtgatg
    10081 ccgatgaaga ggtgccgctc gtcgacaccc ccggcgctga ccaccttcac cagtcggcgc
    10141 gcgacgtgcg gcaggacgag caaactgaag gggtctgcgg gcaggctgtt cgcggtggtg
    10201 acctcgacgg cggctgttcg gcctttcggg tacgtcaggt gcaggtcgta ggctccctga
    10261 cgggtgccga cgtcgtgggc acgaacggtc ccgcccgtca cgtgctcgag gagcagggcg
    10321 gcggcgcgct cgtcggggcg cagtccgtcc aggcggcgcg ggttgctccc catgcagccg
    10381 cacgctaccg gccgggaagg cgttcgtccc cggaatgcag gggcgatgcc gtgggcgcgg
    10441 caccagcggc gggcgcgcgg acggcatccc acgatgcgtg aaacgccgga accgcgctgc
    10501 cccgtgaggg gcagcgcggt tccggtgaag gatggcctgc tcagatgtcg aagtacatct
    10561 cg
//
LOCUS       DAOSOC010000079        10549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_6295_length_10549_cov_9.268249, whole genome shotgun sequence.
ACCESSION   DAOSOC010000079 DAOSOC010000000
VERSION     DAOSOC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6295_length_10549_cov_9.268249"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PL014_07440"
     CDS             <1..878
                     /locus_tag="PL014_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOT56959.1"
                     /translation="ADFVIEAVFEEMAVKQQVFAEVEAVVSPECVLATNTSSLSITEM
                     ASGLEHPGRVVGFHFFNPVAVMPLLEIIPGEATDESALATAFATGRALGKTTIRVKDS
                     ASFVVNRLLGAFMGEFSRIVDEGTPVATADRAVAGLAPMPPFVLLGLVGPAIALHNSE
                     TLHRAFGDRFHVSPSLRRLVDSGKRGYYLMEGGRPVPDPEVLAMAEQPENPVELSAEQ
                     ARERILGVLATEVGLMLSEGVVAAPMDIDLAMITGAGFQFWNGGLCPLLDREGVSERV
                     LGRRLLPAGVASVPA"
     gene            978..>1156
                     /locus_tag="PL014_07445"
     CDS             978..>1156
                     /locus_tag="PL014_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT56960.1"
                     /translation="MIYDGPVTVTSTSDTYLTRIGTLIRDARRHQGLTQSELADLLGT
                     SQSAVARIEQGKQNLS"
     assembly_gap    1157..1256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1257..2201
                     /locus_tag="PL014_07450"
     CDS             <1257..2201
                     /locus_tag="PL014_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT56961.1"
                     /translation="IEPHLSALKPFGLDVAATHGFYEATVDAARSPERAIVLTERGDT
                     VTENALMAAARNPGTTIIRNASPNYMVQDLCFFLQALGVTIEGIGSTTLTVTGVEHID
                     VDIEYFPSEDPIEAMSLIAAAVVTESTITIERVPIEFLEIELATLEEMGLRFDMTSEY
                     RAMNGHTRLVDLTIHPSELKAPVDKIAPMPFPGLNIDNLPFFALIAACAEGTTMIHDW
                     VYENRAIYLTELTKIGGQVQLLDPHRVMITGPTPKWRAAEVMCPPALRPGVVILLAML
                     AAPGTSVLRNVYVINRGYEDLAERLNALGATIETFRDI"
     gene            complement(2440..4659)
                     /locus_tag="PL014_07455"
     CDS             complement(2440..4659)
                     /locus_tag="PL014_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOT56962.1"
                     /translation="MEDDSAGCMTHGSVIRVSDEGDRAVIRDSDEGDRACVDEAGVAA
                     TEASVRAQLDDLSLRVTHLADDIERVSGAWARPDLASALANCQGLINTLTAVQDVAIT
                     RLAAIEEEWLEDGLVVESRRAPGHIALDAPDVVAGALRVSHLQAQRRVRTAVRLVSEV
                     LDGAGTTTESTGEPESMGDSTETGESGESMDAGAAVVDGAGDDRSSGMSGVHRAMRDG
                     LLDGYSASVLDAELEEAPGEVASAVLAAIEDRFATDAPSALRQRCRRALARISPDLLR
                     RRAQRAREECGLKRWAEAPGVDRWTGSFPSERAAMGWAAVDALARRYVKDGLCERLEQ
                     ARGQALMDLVTANATIDVTLVFTTAEEPDAGGARVDRPQAPTAPERAGTSPAQAFPRE
                     PMRIGERDAASSTPGRPALGAGEPSRSSAATPRAERAGENRDVATVGLGTVVGATDAG
                     LVEAFGPSAGDPLLVSRSWLDALARGEAAVRRAACHPSTGALLGHGAMATDAYRPGEK
                     LKALVRLRDGRCRFPGCSVSARFCDLDHARPWPNGPTAAENLMCLCRRHHRIKQRPGW
                     QVRLAVDGTATWTDPVGRSRTTWPRNHLDLLALPMPLALPMPLAQARSRVHGAVPVPA
                     ADVGVLGEEASTLETVMEVLDDGYQRWPRFGQVSVAGVVSRTMSVSQGVLVSRGVPVS
                     RVVPGPRTTPGDLIRAGRDHPRLHYRDVRERRRPCHRREGRWGAQTAPEPGVDPPPF"
     gene            complement(4814..5224)
                     /locus_tag="PL014_07460"
     CDS             complement(4814..5224)
                     /locus_tag="PL014_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOT56963.1"
                     /translation="MGGAQGPGLMDGGAQGRIAVVGQMQARLATERGLLVSRIEQMEA
                     DKARLVAASVDSNADDEHDPEGQTIAFERSQLEALTDRLRQHVTEVEAAQARLAAGRY
                     GTCEVCGEQIDGARLEVRPTARTCVDHASGRRRR"
     gene            complement(5197..5574)
                     /locus_tag="PL014_07465"
     CDS             complement(5197..5574)
                     /locus_tag="PL014_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56964.1"
                     /translation="MSDEADSVGADGADGQVEVGEVEVGEVEVGAVGAVDVGAMAITV
                     AIPEHLRWTDTRRDVEHTLLTLTVRLLPDGHLAVKAYGRPTAGGRSPYVSFPVPDDPA
                     ITALVEAAGARAGELWAAHKGLA"
     gene            complement(5649..6437)
                     /locus_tag="PL014_07470"
     CDS             complement(5649..6437)
                     /locus_tag="PL014_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT56965.1"
                     /translation="MTSTADAGIDALRSTHDGLVAVVRDLSDEQLVAPSGATEWTVAQ
                     VLSHLGSGAEISAATLRNAVDGAGRLPDGFNQGVWDRWNAMSPRHQADGFVVADAALL
                     ADVEALTAEQRETLQVDLGFLPAPVPFATYAGMRLSEAAAHSWDVRVAYDDDAVIDEP
                     TARVLVRLFGGPMGFLLGFTAKADAVAEPAVVDLVGLDHDLSIGDAVALVPAGGVTTA
                     TLEGGADAVARLLTGRLTAAHTPASVVVSGNVSLEDLRRVFPGY"
     gene            complement(6531..8399)
                     /gene="dxs"
                     /locus_tag="PL014_07475"
     CDS             complement(6531..8399)
                     /gene="dxs"
                     /locus_tag="PL014_07475"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOT56966.1"
                     /translation="MSLLDRIEGPNDLRKLSADQLPELAEEIRRFLVHEVSRTGGHLG
                     PNLGVVELTIAIHRVFDSPNDTVVFDTGHQSYVHKVLTGRHDFSGLRTQGGLSGYPSR
                     AESVHDVVENSHASASLSWAHGIAAGRVVRGETNRHTVAVIGDGALTGGMAWEALNNI
                     AAEPDLPLVIVVNDNERSYAPTRGGLADHLATLRTTRGYERFLDWGKHTLARTPVVGG
                     AMYETLHGMKKGIKDIVAPQGLFEDLGLKYVGPVDGHDEQALEAALRKARAFGGPVLV
                     HVITQKGRGYDPAITDEADQFHAVGVINPETGLPLEIAGRSWTDEFSDEMVRIGAERD
                     DVVAITAAMMIPVGLKDFSNAYPERTFDVGIAEQHALTMAAGLAFSGLHPVVAVYATF
                     LNRAFDQLLMDCAMHRAGVTVVLDRSGITGPDGASHHGMWDMTISGVVPGLHLAAPRD
                     GQQVARAVRAAVDISDAPSVVRFPKGSVGAPIEAVRTVGTVDVLAEPDEAEVDVLLVG
                     VGSMAATAMAAAEKLSAEGVRVRVVDPVWALPVSPDLVTLAGAAGRVAVVEDNVVVGG
                     IGSQVELVLREAGLATPLDQFGIPSEFLDHGSRGQVLDRIGLTPDAVASRLRDRLA"
     gene            complement(8491..8928)
                     /locus_tag="PL014_07480"
     CDS             complement(8491..8928)
                     /locus_tag="PL014_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56967.1"
                     /translation="MFENPPLRAGSPASPARQRAASGSGLAGPPGWPNGVPPPGERGW
                     DHRATAWLLDHCPPDYRLYPAWRRHPVALAWFTVRHLDAQLDAMRSAYREVRVELVDD
                     IGPEGVAQVLSDLECEGVRLLSARRAAGLLLDALRGLEFVPRL"
     gene            9075..>10549
                     /locus_tag="PL014_07485"
     CDS             9075..>10549
                     /locus_tag="PL014_07485"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HOT56968.1"
                     /translation="MTAQTTEGTTGRALRPPRAGRSNGQWAVDGREPLNPNEVWKQED
                     GGLNVRERIEQIYSRDGFASIPPQDLSGRFRWWGLYTQRKPGIDGGRTAQLDDTELSD
                     EYFMLRVRLDGGALTLHQLRVLADISHDFARGTADISDRQNIQYHWIRVEDVPEIWRR
                     LEAVGLQTTEACGDTPRVILGSPLAGIAADEILDPTPVIDEITRRFVGDPELANLPRK
                     FKSAITGHPSLDVVHEINDISLVGVVHPELGAGYDLWVGGGLSTAPRLAERIGVFVAP
                     DEAAEVWLGVIRIFRDYGYRRMRTKARLKFLLAEWGPAKFREVLETEYLARALPDGPP
                     PAPASTPGDHVGIHLQKDGNHYVGAAPVVGRVSGDVLTGLADVMEAAGTDRVRLTPHQ
                     KLVVLDVPPARLDDLVTGLENLGLSVAPSPFRRHTMACTGIEFCKLAIVETKAAAATA
                     IAELEQRLADVTAQIDAPVTLNVNGCPNSCARIQVADIGLKG"
BASE COUNT         1648 a         3844 c         3455 g         1502 t
ORIGIN      
        1 acgccgactt cgtcatcgag gcggtcttcg aggagatggc ggtcaagcag caggtgttcg
       61 ccgaggtcga ggcggtcgtc tcccccgagt gcgtgctcgc gacgaacacg tcctcgctgt
      121 cgatcaccga gatggcgagc gggctggagc acccgggtcg ggtcgtgggc ttccacttct
      181 tcaacccggt ggccgtcatg cccctgctcg agatcatccc cggcgaggcg accgacgagt
      241 ccgcgctggc gacggcgttc gccaccgggc gagcgctcgg caagacgacg atccgggtga
      301 aggactcggc atccttcgtc gtgaaccgcc tgctgggcgc gttcatgggc gagttctccc
      361 gcatcgtcga cgagggcacc ccggtggcca cggccgaccg cgccgtcgcg ggtctggcac
      421 cgatgccgcc gttcgtgctg ctcgggctcg tcgggccggc gatcgctctg cacaacagcg
      481 agaccctgca ccgcgcgttc ggtgaccggt tccacgtctc gccgagcctg cgacggctcg
      541 tcgactccgg caagcgtggc tactacctca tggagggtgg ccgtccggtg cccgacccgg
      601 aggtgctcgc gatggccgag cagccggaga acccggtgga gctcagcgcc gagcaggccc
      661 gtgagcgcat cctcggggtg ctggccacgg aggtcggcct catgctgtcg gagggcgtcg
      721 tcgccgcgcc gatggacatc gacctggcga tgatcaccgg cgccgggttc cagttctgga
      781 acggcgggct ctgcccgttg ctcgaccgcg agggcgtctc cgagcgggtg ctcggtcggc
      841 ggctgctgcc ggccggggtg gcgagcgtcc ccgcctgatc cgacgtcgtc atgccgggtg
      901 gccgcagcag aggttcgacg ctgcggccgc ccggcatccg aacatcccac gtcagcagtg
      961 caggcaatat ctcacatatg atctacgatg ggccagtgac cgtcacctca acgtcagaca
     1021 cgtacctcac ccggatcggc accctcatcc gggacgcacg ccgccaccag gggctgaccc
     1081 agagcgagct cgccgacctg ctcggcacga gccagagcgc ggtcgcccga atcgaacagg
     1141 gcaagcagaa cctgtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatcg
     1261 agccccacct gtccgctctc aagcccttcg ggctcgacgt ggcggccacc catggtttct
     1321 acgaggcgac cgtggatgcc gcccgctccc ccgagcgcgc catcgtcctc accgagcgtg
     1381 gggacacggt gacggagaac gccctcatgg cggcagcccg caacccgggc accacgatca
     1441 tccgcaacgc ctcgccgaac tacatggtcc aggacctgtg cttcttcctg caggcgctcg
     1501 gtgtgacgat cgagggcatc gggtcgacca cgctcacggt caccggcgtg gagcacatcg
     1561 acgtcgacat cgagtacttc ccgtcggagg acccgatcga ggcgatgagc ctgatcgcgg
     1621 cggccgtcgt caccgagtcg accatcacca tcgagcgggt gccgatcgag ttcctcgaga
     1681 tcgagctggc cacgctggag gagatgggcc tgcgcttcga catgacctcc gagtaccggg
     1741 cgatgaacgg ccacacccgg ctggtcgacc tgacgatcca ccccagcgag ctgaaggcgc
     1801 cggtcgacaa gatcgcgccg atgccgttcc cggggctgaa catcgacaac ctgccgttct
     1861 tcgccctgat cgcagcatgt gccgaaggca cgacgatgat ccacgactgg gtgtacgaga
     1921 accgggccat ctacctcacc gagctgacga agatcggcgg ccaggtgcag ctgctcgacc
     1981 cgcaccgggt gatgatcacc ggccccacgc cgaagtggcg cgcagccgag gtcatgtgcc
     2041 cgccggcgct gcgtcccggg gtggtcatcc tgctggcgat gcttgcggca ccgggaacgt
     2101 cggtgttgcg caacgtctac gtcatcaacc gtggctacga ggacctcgcc gagcgcctca
     2161 acgcgttggg cgcgacgatc gagacgttcc gcgacatcta ggaaacgtcg ctgacggtgg
     2221 tctcggacac tgtcgcttcg agcgccgttg cctcgagcac cgttgcgtcg gatgctgccg
     2281 ctgggggccg ttgtctcgga cgacgccgtt gcctcgaacg ctgtcgtatc ggacgccgtt
     2341 gcttggacgt cgctgttgct tcggacgccg tgcggccggg actcatcgct gtcgtaggcg
     2401 ccgaccctct catgcgaact gtgccggacc atcgaaacgt cagaacggag gcggatccac
     2461 acccggctcg ggagccgtct gggcgcccca ccgtccctcg cggcgatggc acgggcgacg
     2521 ccgttcacgc acatcgcgat agtgcaatcg cgggtgatct cggcctgcac ggatcaggtc
     2581 gcccggagtc gttcgcggcc ccggcaccac ccgagacacc gggacgccac gagacaccag
     2641 gaccccttgg gacaccgaca tcgtgcggga caccacgcca gcgaccgata cctgcccgaa
     2701 gcgcggccac cgctggtagc cgtcatccag cacctccatc accgtttcga gggtgctggc
     2761 ttcctcgccc aggacgccaa cgtcggccgc cgggactgga actgcaccgt gcacccgtga
     2821 cctcgcctgt gcgaggggca tcggcagtgc gaggggcatc ggcagtgcga ggagatccag
     2881 gtgattccgc ggccaggtgg tgcgactcct gcccaccgga tcggtccacg tcgcggttcc
     2941 atcgactgcc agacgcacct gccagccggg tcgctgtttg atccggtgat gccgtcggca
     3001 cagacacatg aggttttctg cagcggtggg cccgttgggc catggccggg catgatcgag
     3061 gtcgcagaag cgagccgaca ccgagcaccc gggaaaccgg cagcggccgt ctcgcaaccg
     3121 gaccagagcc ttcagcttct cccctgggcg atacgcgtcc gtcgccatgg cgccgtggcc
     3181 gagcaacgcc ccggtcgagg ggtggcatgc ggctcggcgc accgcggcct cccctcgtgc
     3241 gagggcgtcc aaccacgagc gggaaaccag cagcgggtcc cctgcactcg ggccaaaggc
     3301 ctccacgagg ccagcgtccg tcgctccgac aaccgtcccc aagcccaccg tcgcgacatc
     3361 gcggttctcc ccggctcgct ccgcccgagg cgtggcagca gacgacctgg atggctcgcc
     3421 tgcgccgagt gcagggcgac caggcgtgga cgaagcggca tcgcgctcac cgatgcgcat
     3481 gggctcccgc ggaaacgctt gggctgggct ggtacccgcg cgctcaggcg ccgtcggcgc
     3541 ttgtggacga tcgacgcgcg ctccaccggc atcgggctct tcggcggtgg tgaagacgag
     3601 cgtcacatcg atggtggcgt tggcggtgac gagatccatg agggcctggc ccctggcctg
     3661 ctcaagacgc tcacacagac cgtccttgac gtaacggcga gcgagagcat ccaccgcggc
     3721 ccagcccatg gcagccctct ccgaggggaa ggagccggtc cagcgatcga cacccggcgc
     3781 ctcggcccat cgcttgagcc cacattcctc acgcgctcgc tgagcccgcc gcctcaacag
     3841 gtcagggctg atccgagcca gggcgcgacg acaccgctgt cgcagggccg acggagcgtc
     3901 ggtggcaaag cgatcctcga tcgcggcaag caccgcggag gccacctcgc caggtgcctc
     3961 ctcgagctcg gcatcgagaa cactcgccga atacccatcg agcagtccgt cacgcatggc
     4021 gcggtggacc ccggacatcc ccgaactcct gtcatcacca gcaccatcga ctaccgcagc
     4081 cccggcatcc atcgactcgc ctgactcccc cgtctccgtg gagtccccca tcgactcggg
     4141 ctcgcctgtc gattccgtgg tcgtacccgc gccatccagc acctcggaga ccagccggac
     4201 agcagtgcgg acccgccgct gcgcctgcag atgggacacc cgcaaggctc cggccacgac
     4261 gtcaggtgcg tcgagggcga tgtgccccgg cgcccggcgg gactcgacga ccaacccgtc
     4321 ctccagccac tcctcctcga tcgccgccag ccgtgtgatc gcgacgtcct gaactgcggt
     4381 cagggtgttg atcaaccctt ggcagttcgc cagggccgat gcgagatcag gcctggccca
     4441 tgcaccgctg accctctcga tgtcgtcggc aaggtgggtc acacgcagtg aaaggtcgtc
     4501 gagttgcgca cgcacactcg cctcggtggc ggccacaccc gcctcatcga cgcacgcacg
     4561 gtcgccctcg tcgctgtcac ggatcactgc acgatcgccc tcgtcgctga cacggatcac
     4621 cgaaccgtgc gtcatgcacc ccgcgctgtc gtcctccacg ttcaccaccc ccttcccgat
     4681 cacgctctcc cccagccgga tccgccaaca atccgcccgc cccgccatcg ccctgaagcg
     4741 atggatagaa cctacaggcg gccaccgaca gggccctcgc gaggggcgtc accccccgag
     4801 gtcgccgtca gggtcagcgc cgacgcctgc cgctggcgtg gtcgacacac gtgcgcgccg
     4861 tcggacgaac ctcgagcctg gcaccgtcga tctgctcccc gcagacctcg cacgtgccat
     4921 accgtcctgc cgcaagacgc gcctgcgccg cctccacctc cgtcacgtgc tggcgcagcc
     4981 gatccgtcag cgcctccagc tgcgatcgtt cgaacgcgat ggtctgaccc tcggggtcgt
     5041 gctcgtcatc ggcattggaa tcaacggatg ccgccacgag gcgcgccttg tcggcctcca
     5101 tctgctcgat gcgactcacc aacagcccgc gttcggtggc gagccgagcc tgcatctgac
     5161 cgaccacggc aatgcgaccc tgcgcgccac catccatcag gccaggccct tgtgcgccgc
     5221 ccacagctcg ccggcccgtg ctccagcagc ctcgaccagc gcggtgatgg cggggtcgtc
     5281 aggcaccggg aacgacacgt acggcgatcg accaccagcg gtgggtcggc cgtatgcctt
     5341 cacggcaagg tggccatcgg ggagcaggcg aaccgtgagc gtcagcaggg tgtgctcgac
     5401 gtcgcggcgc gtgtcggtcc agcgcaggtg ctcggggatg gccacggtga tcgccatggc
     5461 gcccacgtcg acggcgccca cggcgcccac ctcgacctcg cccacctcga cctcgcccac
     5521 ctcgacctgg ccatcagcgc cgtcagcgcc cacggagtct gcttcgtcgc tcatgacgac
     5581 atcgtgccgc agcaccctga caccactctc aacactcttc ggcaccggga gacggcacct
     5641 cgagttcctc agtaccccgg gaatacccgg cgaaggtcct ccaacgagac gttgccggac
     5701 acgacgacgg atgccggggt gtgcgcagcc gtgaggcgcc cggtgagcag ccgcgccacg
     5761 gcatccgccc caccctcgag ggtcgcggtg gtgaccccgc ccgcgggaac gagcgccacg
     5821 gcatccccga tcgacaggtc gtggtcgagg ccgacgaggt cgacgacggc gggttcagcc
     5881 acggcatcgg ccttcgccgt gaagccgagg aggaacccca tcggaccgcc gaacagccgc
     5941 acgagcaccc gggccgtcgg ctcgtcgatg accgcatcat cgtcgtaggc gacccgcacg
     6001 tcccacgagt gggccgccgc ctcgctgagc cgcatcccgg cgtacgtggc gaagggaacc
     6061 ggcgcaggaa ggaacccgag gtcgacctgg agggtctcgc gctgctcagc cgtcagggcc
     6121 tcgacgtctg ccagcagagc cgcatcggcg acgacgaagc cgtcagcctg gtggcgcggg
     6181 gacatcgcgt tccatcggtc ccacaccccc tggttgaagc catcgggcag ccgcccagcc
     6241 ccgtcgaccg cgttgcgcag cgtcgccgcc gagatctcag cgccactgcc gaggtgggaa
     6301 agcacctgcg cgacagtcca ttccgtggcc ccggacggcg ccaccaactg ctcgtcggac
     6361 aggtcgcgaa cgacggcgac gaggccgtca tgggtggagc gaagggcgtc gatgccagcg
     6421 tctgcggtgg aggtcatgct cccgcaaccg cacacctcac cctcgttgtt ccccacgacg
     6481 cagacatcgg atgccgtgtg gccgccgcgc gctcaccgcg agccgatcgc tcaggcgagg
     6541 cggtcacgca ggcggcttgc cacggcatcc ggcgtgagac cgatgcggtc gagcacctgg
     6601 ccgcgcgagc cgtggtcgag gaactcgctc gggatgccga actggtcgag cggtgtggcc
     6661 aggccggcct cgcgcagcac cagctcaacc tgggagccga tgccacccac gacgacgttg
     6721 tcctcgacga cggcgacccg gcccgctgcc ccagcgaggg tcacgaggtc cgggctgacc
     6781 ggcagcgccc acaccgggtc gacgacgcgc acgcgcaccc cttcggccga gagcttctca
     6841 gccgcagcca tcgcggtggc cgccatcgac ccgaccccca cgaggagcac gtcgacctct
     6901 gcctcgtcgg gctcggccag gacgtcgacg gtgccgacgg tgcgaacggc ctcgatcggg
     6961 gcacccaccg accccttggg gaagcgcacc accgaggggg catcggagat gtccacggcg
     7021 gcgcgcaccg cgcgggccac ctgctgcccg tcgcgtggtg ccgcgaggtg cagccccggc
     7081 acgacaccgc tgatcgtcat gtcccacatg ccgtggtgcg aggcgccgtc ggggccggtg
     7141 atgcccgagc ggtcgagcac gacggtgacc ccggcgcggt gcatcgcgca gtccatgagc
     7201 agctggtcga aggcgcggtt gaggaacgtc gcgtagacgg ccacgaccgg gtgcaggccg
     7261 gagaacgcca gaccggcggc catcgtcagc gcgtgctgct cggcgatgcc gacgtcgaac
     7321 gtgcgctcgg ggtaggcgtt gctgaagtcc ttgaggccga cggggatcat catcgccgcg
     7381 gtgatggcga cgacgtcgtc acgctctgcc ccgatgcgca ccatctcgtc actgaactcg
     7441 tcggtccagc tgcggccggc gatctccagc gggaggccgg tctcggggtt gatgaccccg
     7501 acggcgtgga actggtcggc ctcgtcggtg atggcggggt cgtagccgcg ccccttctgg
     7561 gtgatgacgt gcacgagcac cgggccaccg aaggcccggg ccttgcgcag cgcggcctcg
     7621 agggcctgct cgtcgtgccc gtcgaccggg ccgacgtact tcaggccgag gtcctcgaag
     7681 agcccctggg gcgcgacgat gtccttgatg cccttcttca tcccgtgcag cgtctcgtac
     7741 atcgcgccgc cgacgacggg ggtgcgcgcc agggtgtgct tgccccagtc gaggaagcgc
     7801 tcgtagccgc gggtcgtgcg cagggtcgcc aggtgatcgg cgaggccgcc cctggtcggc
     7861 gcgtaggagc gttcgttgtc gttgacgacg atgaccaggg ggaggtccgg ctcggccgcg
     7921 atgttgttca gcgcctccca ggccatgccg ccggtcaggg cgccgtcacc gatgaccgcg
     7981 acggtgtggc ggttggtctc gccgcgcacc acacggccgg cggcgatgcc gtgggcccac
     8041 gacagcgagg ccgaggcgtg ggagttctcg acgacgtcgt gcacggactc ggcgcggctc
     8101 gggtaaccgg acaggccacc ctgggtgcgc agccccgaga agtcgtggcg cccggtgagc
     8161 accttgtgca cgtaggactg gtggccggtg tcgaacacca cggtgtcgtt cggcgagtcg
     8221 aatacccggt ggatcgcgat ggtcagctcg acgacgccca ggttcgggcc gaggtggccg
     8281 ccggtgcgcg agacctcgtg gacgaggaaa cggcggatct cctcggccag ctcgggcagc
     8341 tggtcggcac tcagcttcct gaggtcgttg gggccctcga tgcggtcgag cagactcacc
     8401 ggtgcccacc tcctgatctg atgcaacatc cacgacacga accccagaag tctaggcgct
     8461 cgacccggtc gacccttgac aaggcaccga tcacagccgc gggacgaact ccagaccgcg
     8521 cagggcgtcc aggagcagcc ccgcagcccg cctggccgac aacaaccgca ccccttcgca
     8581 ctcgaggtcg ctcagcacct gggccacccc ctcggggccg atgtcgtcga cgagctcgac
     8641 ccgcacctcg cggtaggccg agcgcatcgc gtcgagctgg gcgtcgaggt ggcgcaccgt
     8701 gaaccacgcc aacgccaccg gatgccgtcg ccacgccgga tagagccgat agtccggtgg
     8761 gcagtggtcg agcaaccagg ccgtggcccg gtggtcccag ccgcgctcac ccggtggtgg
     8821 gacaccgttc ggccatcccg gtgggccagc caggcccgag ccggatgccg ctcgctggcg
     8881 cgccggggag gccgggctcc cagcgcgcag cggcggattc tcgaacacgt gttcgagcat
     8941 acgccgggat cacatggtgg acgaagactc ttcaggtaat cgagcgttat acatttgatg
     9001 actgttgcta atcctttggc agcgtgaccg accccgttcg gccctgctga ccacgacgac
     9061 ccaccggtgg agacatgacc gcacagacga ccgaaggcac gaccgggcgg gctcttcgcc
     9121 cccctcgggc cgggcgctcg aacggccagt gggcggtcga cggccgggag ccgctgaacc
     9181 ccaacgaggt gtggaagcag gaggacggcg gcctcaacgt ccgcgagcgc atcgagcaga
     9241 tctactcgcg cgacggcttc gcctcgatcc ccccgcagga cctcagtggc cggttccgct
     9301 ggtgggggct gtacacccag cgcaagcccg gcatcgacgg cggccgcacg gcccagctcg
     9361 acgacaccga gctctccgac gagtacttca tgctgcgggt gcgcctcgac ggcggcgccc
     9421 tgaccctgca ccagctgcgc gtgctcgccg acatcagcca cgacttcgcc cgcggcaccg
     9481 ccgacatcag cgaccggcag aacatccagt accactggat ccgcgtcgag gacgtccccg
     9541 agatctggcg gcgcctcgag gccgtcgggc tccagacgac cgaggcctgc ggtgacaccc
     9601 cccgcgtcat cctcggcagc ccgctcgccg ggatcgccgc cgacgagatc ctcgacccga
     9661 ccccggtcat cgacgagatc acccgccgct tcgtcggtga ccccgagctc gccaacctgc
     9721 cccgcaagtt caagtccgcc atcaccggcc acccgagcct ggacgtcgtc cacgagatca
     9781 acgacatctc cctcgtcggc gtcgtccacc ccgagctggg cgccggctac gacctgtggg
     9841 tcggtggcgg cctgtccacg gcgccgcgcc tggccgagcg gatcggcgtc ttcgtcgccc
     9901 cggacgaggc cgcagaggtc tggctgggcg tcatccgcat cttccgcgac tacggctacc
     9961 gccggatgcg caccaaggcc cgcctgaagt tcctgctcgc cgagtggggc cccgcgaagt
    10021 tccgcgaggt gctcgagacc gagtacctcg cacgcgccct gcccgacggc cctccccccg
    10081 ccccggcatc cacccccggc gaccacgtcg gcatccacct ccagaaggac ggcaaccact
    10141 acgtcggcgc cgccccggtc gtcgggcgcg tcagtggcga cgtgctcacc ggcctcgctg
    10201 atgtcatgga ggccgccggc accgaccggg tgcgcctgac cccgcaccag aagctcgtcg
    10261 tcctcgacgt cccgcccgcc cgtctcgacg acctcgtcac cggcctcgag aacctgggcc
    10321 tgtcggtcgc accgagcccg ttccggcgcc acacgatggc gtgcaccggc atcgagttct
    10381 gcaagctcgc gatcgtcgag accaaggcgg ctgccgccac ggccatcgcc gagctcgagc
    10441 agcgcctggc cgacgtcacc gcgcagatcg acgccccggt caccctgaac gtcaacggct
    10501 gccccaactc gtgcgcccgc atccaggtcg ccgacatcgg cctcaaggg
//
LOCUS       DAOSOC010000080        10453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_6368_length_10453_cov_9.212637, whole genome shotgun sequence.
ACCESSION   DAOSOC010000080 DAOSOC010000000
VERSION     DAOSOC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10453
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6368_length_10453_cov_9.212637"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PL014_07490"
     CDS             <1..1284
                     /locus_tag="PL014_07490"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT56969.1"
                     /translation="APDLIITEIGGTVGDIESLPFLEAARQVRHDLGRDKVFFLHVSL
                     VPYLAPSGELKTKPTQHSVAALRQVGIQPDALVLRADREIPESIKRKISLMCDVDTEA
                     VAAAVDAPSIYDIPKVLHREGLDAYVIRRLGLTFRDVDWTDWDLLLERVHEPEHEVTV
                     ALVGKYIDLPDAYLSVTEALRAGGFHHDAKVQIRWVASDTCDTEAGAQRALGGVDAVL
                     VPGGFGVRGIEGKVGALRWARTHLVPTLGICLGLQCMVIEYARDVAGIEGASSTEFDP
                     KSTAPVIATMEEQKAFVEGAGDLGGTMRLGSQRAALLEGSVVAGAYGATFADERHRHR
                     YEVNNDYRQQLEDAGLVFSGTHPELGLVEFVELPADVHPYYVSTQAHPEFTSRPTRAH
                     PLFAGLVGAAIEAQRASRLVEVERPRHHEAADAHA"
     gene            1277..1912
                     /locus_tag="PL014_07495"
     CDS             1277..1912
                     /locus_tag="PL014_07495"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT56970.1"
                     /translation="MPDELRDQLASLPVRSCVRSFTGHVWNIVTEDVDLGEGGVVTRD
                     FVDHPGAVGILALDEQDRVVLVQQYRHPVGAYEWELPAGLLDVDGEAPHLTAARELME
                     EADLEAAQWHLLLDHYTSSGGSSESVRVFLARGLTDVPVESRHARDGEELGMPVRRVP
                     LDELVESVLAGDVHNGTLMVSALAAQRLRERGWVGTRSVDEPWPTRPRRRG"
     gene            1909..2364
                     /locus_tag="PL014_07500"
     CDS             1909..2364
                     /locus_tag="PL014_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HOT56971.1"
                     /translation="MSERAFGDRSAAARMPSADRRCPCGGVPAGAALSACCGPVVAGE
                     LAASTAEALMRSRYTAYVLGDGDHLFRTWHPRTRSDDADPDPRVRWEGLSVLDVVAGG
                     PQDTDGIVEFRARWVSADDGPVRRGQLHERSRFERRAGRWFYVEAVDKA"
     gene            2428..3945
                     /locus_tag="PL014_07505"
     CDS             2428..3945
                     /locus_tag="PL014_07505"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOT56972.1"
                     /translation="MAASRTTVVRGLATAVGIVSLVSVTGLATAAPGDPGKPRFTAGS
                     EGVGDNYFPYSGNGGYDVSHYDLAIDYTPPAPSPAPLEGQLSGVATIDLTATADLDRF
                     NLDLRGMTVSAMTVNGKRATGITPPAPGAEVDGAAYWQVQDDAERRWELTVQPRPKLK
                     KGQSVRLVVTYGGTTTRPTDIEQAPYGWYTTRDGAIVVSEPDGSMTWYPVSDHPRDKA
                     TYSFRITVPEGKVAVANGLPDGPPVTANRRTTWSWDAVDEQASYLTTASVGDFEVLPV
                     TYSASGVPIHDFVDRKISASRRATTNASLALQPRMIDFFESRFGPYPFSAFGSSVDND
                     SVGYALETQTRPVYSGSASQGTVAHELAHQWLGNAVSPKDWKHIWLNEGWATYATWLW
                     NEENGIRTAQAAFDSWYAPARTPAYWALPIDDPGPMGLFAGQVYDRGAGTLHALRLKV
                     GDEAFFAGVQTWVERYDDSTATTADFQAVYEEVSGQDLDGFFQEWLATPAKPSYP"
     gene            complement(3966..5489)
                     /locus_tag="PL014_07510"
     CDS             complement(3966..5489)
                     /locus_tag="PL014_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56973.1"
                     /translation="MNQPLPRRTALRAAAVLVVGSVAATTTACTSDSSGGSDDGDPDA
                     GSTSLVVAADQRAGVVDVDASPHEQALAASRVLLDRAGVVVVASADTDVAAAAELATA
                     HALPVLVAGPGLAAELDRLGVGTVVRVGAGAPEGVDESAGLVVVDGSDAGAVAALPGL
                     PPRPKAHGAVVLLRPGGALPPAVAATADAAGADRVTVQHGDVRATPDAMGPVRAGSGP
                     TLALGSDLGPAERVAQRVRTVRRAVELPGGGFVPFPGRRMIALYGHPQTKALGMLGEQ
                     PPDQAVRRARSLAKEYTALSKEPVIPAFELIASVASQAAGEDGSYSRRTPIEVLLPWV
                     EAAEQAGIYVVIDLQPGRTDFLTQAKFYEPLLRRPSVGLALDPEWRLEPDQKHLRQIG
                     SVGVDEVNRVGAWLAALVREHDLPAKVLTLHQFRPSMITDRERLDTTLDEIQWLVHAD
                     GQGRQGAKQSTWKRLRRDLPDGVWLGWKNFEDEDLPMLTPEQTMAQVSPTPWFVSYQ"
     gene            5661..7454
                     /gene="aspS"
                     /locus_tag="PL014_07515"
     CDS             5661..7454
                     /gene="aspS"
                     /locus_tag="PL014_07515"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT56974.1"
                     /translation="MLRTHEAGTLRAAHTGQTVTLTGWVAKRRDHGGVAFIDLRDASG
                     VVQVVARDEVLTGAAHDLRSEYVVKVVGEVTAREDRNINPDLPTGEVEVVAGAIEVLN
                     ESAPLPFQVDERVTVGEEARLKHRYLDLRRPGTRSAGHAIRLRSKVSAAARRVLEARD
                     FVEIETPTLTRSTPEGARDFLVPARLAPGSWYALPQSPQLFKQLLMVAGMERYYQIAR
                     CYRDEDFRADRQPEFTQLDIEMSFVEQDDVLELGEAVVREIWQLIGVDLPTPFRRMTY
                     AEAMRRFGSDKPDLRFGQELVECTDYFADTPFRVFQAEYVGAVVMPGGASQPRKQLDA
                     WQEWAKQRGARGLAYVLVQEDGTLTGPVAKNLSETELEGLAAHVGAQPGDCVFFAAGS
                     TRSSRALLGAARLEIGKRCGLIDENAWEFLWVLDAPLFEPASEAVAAGDVAVGSGAWT
                     AVHHAFTSPKAEFLDTFDTDPGPALAYAYDMVLNGNELGGGSIRIHRRDVQERVFQVM
                     GLSREEAQEKFGFLLDAFAFGAPPHGGIAFGWDRIVMLLGGFDSIRDVIAFPKSGGGY
                     DPLTEAPAPITPEQRKEAGIDSRPPASEPPA"
     gene            complement(7464..8234)
                     /locus_tag="PL014_07520"
     CDS             complement(7464..8234)
                     /locus_tag="PL014_07520"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT56975.1"
                     /translation="MTAVRTLTTRAEFEEATSRHPVAVLDVGAGFVGPAFAVDHAGQT
                     SVLFHRRSDHGVPGSSALATEGGLAILLDDPAVRAFVTGEGHRHLSVPRGLVGLVEAR
                     LDLGTRGGEWDWMWTSTSPAPVRGEDHVVVLDPSSRQEAEAFLAAHSPRTHGQPFARP
                     DQYWVGVRDPHTGALLATGCSEPCAAGTPTLAGIAVDASQRGRGWGAAVTAHLTRRAV
                     EQTGACALGMFADNDSARRVYERLGFTTGMRWTSRWFG"
     gene            complement(8231..10435)
                     /locus_tag="PL014_07525"
     CDS             complement(8231..10435)
                     /locus_tag="PL014_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT56976.1"
                     /translation="MSRSARHGSPALERWGRHVARHAKAIVGFWVLFIVVGFAVALGA
                     TGTPSLFSRLDSGEIVVAGENQDGRDLLAQGGGSGFSTYTQTLTGVDLSDPAVASAAA
                     TAVQDLSAIEHVESAVNPFVVPGGPSTPDALRFVLDGDPASGGFATVVTFDEANTAEQ
                     EAAATDQVDAVFDGLAEDTGATGDQRGGLRSLVDRIIEQVKIDGQRGEGIALPVSFLV
                     MVVVFGGFLAAGFPVLGAIASIAGALASLLGLSYLLDLDASVVNVVTVLGLGLCIDYG
                     LLVVSRFREEMRALLQGTPATDATHAQVVAATAATMNRAGRTVIFSALTVAISLSGLL
                     VLQIPFIRAVSVAGVSVVLIALAVALTLIPALCVLGARRLLKRGTETASDVGVFSRLA
                     ERVHRMPWVVIAAVSAVLVVMALPALRLELTSSGPELLPVGTPERTFYEDFRAGYPLL
                     AGAEVLLVSRAPVAEVQAWADTAADRPGVESVDPVRELDNGVVTVGFQTGDSGTGEAS
                     RDLVDSLRSDRAPFDAYVVGQASGIVDFRATVADRALWAAGLVVLATLVLLFLMTGSV
                     VIPIKALVMNVLSLGAALGVTVWIFEDGHLEGLLRFASTGALENTIPLLVLAFGFGLS
                     MDYEVFLLSRIVELHEQGHDTRSAVTLGLQRSGRIITSAALLMVIVFSGFAAGDLLIM
                     KQMGVALVLAIVIDATLVRMLLVPATMSVLGSANWWAPAPLRRLHERWGITE"
BASE COUNT         1542 a         3751 c         3784 g         1376 t
ORIGIN      
        1 gcgcccgacc tcatcatcac cgagatcggc ggcaccgtcg gcgacatcga gtcgctgccc
       61 ttcctcgagg ctgcccgcca ggtgcgccac gacctgggcc gggacaaggt gttcttcctg
      121 cacgtctcgc tggtgcccta cctcgccccg agcggtgagc tgaagaccaa gccgacgcag
      181 cactcggtcg ccgccctgcg ccaggtcggc atccagcccg acgccctggt cctgcgggcg
      241 gaccgcgaga tccccgaatc catcaagcgc aagatcagcc tgatgtgcga cgtcgacacc
      301 gaggccgtcg cggccgccgt cgacgccccc agcatctacg acatcccgaa ggtgttgcac
      361 cgcgaggggt tggacgccta cgtcatccgc cgcctcgggc tgaccttccg cgacgtcgac
      421 tggacggact gggacctgct cctcgagcgg gtccacgagc ccgagcacga ggtgaccgtc
      481 gccctggtcg gaaagtacat cgacctgccc gacgcctacc tctcggtcac cgaggccctg
      541 cgggcaggcg gtttccacca cgacgccaag gtccagatcc ggtgggtcgc ctccgacacc
      601 tgcgacaccg aggcgggggc gcagcgcgcc ctcggcgggg tggatgccgt gctcgtgccc
      661 ggaggcttcg gcgtgcgggg tatcgagggc aaggtcggcg cgttgcgctg ggcccgcacc
      721 cacctcgtcc cgacgctcgg gatctgcctg ggtctgcagt gcatggtcat cgagtacgcc
      781 cgcgacgtcg ccgggatcga gggcgcgagc tcgaccgagt tcgaccccaa gagcacggct
      841 ccggtcatcg cgacgatgga ggagcagaag gcgttcgtcg agggtgccgg cgacctgggc
      901 gggacgatgc ggttgggttc gcagcgggct gcgttgctcg agggatcggt cgtcgccggt
      961 gcgtacggtg cgacgttcgc cgacgagcgg caccgccacc gctacgaggt caacaacgac
     1021 taccggcagc agctcgagga cgccgggttg gtgttcagcg gcacccaccc cgagctgggg
     1081 ctcgtcgagt tcgtcgagct gccggccgac gtgcacccct actacgtctc gacacaggct
     1141 caccccgagt tcacgtcgcg cccgacgcgg gcccacccgc tgttcgccgg tctcgtcggc
     1201 gcggcgatcg aggcccagcg ggcctcacgg ctcgtcgagg tggagcgtcc gcgccaccac
     1261 gaggctgccg acgcccatgc ctgacgagct gcgtgaccag ctcgcgtcac tgcccgtgcg
     1321 gtcgtgcgtg cggtccttca cgggccacgt gtggaacatc gtcaccgagg acgtcgacct
     1381 cggtgagggc ggggtcgtga cccgggactt cgtcgaccat cccggggcgg tcggcatcct
     1441 cgccctcgat gagcaggatc gcgtcgtgct cgtccagcag taccggcacc cggtcggtgc
     1501 ctacgagtgg gagctcccgg cgggtctgct cgacgtcgac ggagaggcac cgcacctcac
     1561 tgcggcgcgc gagctcatgg aggaggccga cctcgaggcg gcgcagtggc acctgctcct
     1621 cgaccactac acgagcagcg gcgggtccag cgagtccgtg cgggtcttcc tcgcccgcgg
     1681 actcaccgac gtcccggtgg agtcgcgcca cgcgcgcgac ggggaggaac tcgggatgcc
     1741 ggtgcgtcgc gtcccgctcg acgagctcgt ggagtccgtc ctcgccggcg acgtgcacaa
     1801 cggcaccctc atggtctcgg ccctggcagc ccagcggctg cgcgagcggg gctgggtcgg
     1861 cacgcgctcg gtcgacgagc cgtggccgac gcggccccga cgccgcggat gagcgagcga
     1921 gccttcgggg accggtcggc ggcggcgcgg atgccgtcgg cggaccggcg gtgcccgtgc
     1981 ggtggcgttc ctgccggggc cgcgctgagc gcgtgctgcg gcccggtcgt cgccggtgag
     2041 ctggcggcat ccaccgccga ggcgctgatg cgctcgcggt acaccgccta cgtgctcggc
     2101 gacggtgacc acctctttcg cacctggcac ccccgcaccc gcagcgacga tgccgacccg
     2161 gacccgaggg ttcgctggga ggggctcagc gtgctcgacg tcgtggcggg tggcccgcag
     2221 gacaccgacg gcatcgtgga gttccgcgcc cgctgggtca gtgccgacga cgggccggtg
     2281 cggcgcgggc agctgcacga gcgcagtcgc ttcgagcgcc gcgccggcag gtggttctac
     2341 gtcgaagccg tcgacaaggc ttgacgagac ctgttctcct gcaccgcgcg ctgccatact
     2401 cgggcatccc acgagcctgg aggccgcatg gccgcgtcac gaaccaccgt cgtccgaggg
     2461 ctggccacag ccgtcggcat cgtgagcctg gtgagtgtca ccgggttggc caccgcagcg
     2521 cccggtgacc ctggcaagcc gcgcttcacc gcgggctccg agggcgtggg ggacaactac
     2581 ttcccctact cgggcaacgg tggctacgac gtctcgcact acgacctggc catcgactac
     2641 acgcctccgg caccctcgcc ggcccccctg gaaggccagc tctcgggagt tgcgacgatc
     2701 gacctcaccg cgacggccga tctcgaccgg ttcaacctcg acctgcgggg gatgacggtc
     2761 tcggcgatga cggtcaacgg caagcgcgcc accggcatca cgccacccgc gcccggtgcc
     2821 gaggtcgatg gagccgcgta ctggcaggtg caggacgacg ccgagcgccg gtgggaactc
     2881 accgtgcagc cacggcccaa gctcaagaag ggccagagcg tccgactcgt cgtcacctac
     2941 ggggggacca ccacgcgccc gaccgacatc gagcaggctc cctacggctg gtacaccacc
     3001 cgggacgggg cgatcgtcgt cagcgagccg gacggctcga tgacgtggta ccccgtgagt
     3061 gaccacccac gcgacaaggc cacgtactcg ttccggatca ccgtgcccga gggcaaggtg
     3121 gcggtggcca acgggcttcc ggacggccct ccggtgactg ccaacaggcg cacgacctgg
     3181 tcgtgggatg ccgtggacga gcaggccagc tacctcacga ccgcgtcggt gggtgacttc
     3241 gaggtgctcc cggtgacgta ctcggcgagc ggggtgccca tccacgactt cgtcgacaga
     3301 aagatctccg cgagccggcg cgccacgacc aacgccagcc tggccctgca gccccggatg
     3361 atcgacttct tcgagtcgag gttcggcccc tacccgttca gcgcgttcgg ctccagcgtc
     3421 gacaacgaca gcgtgggcta cgcgctcgag acgcagaccc gcccggtgta ctccggctca
     3481 gccagtcagg gcaccgtggc gcacgagctg gcgcaccagt ggctgggcaa cgccgtcagc
     3541 ccgaaggact ggaagcacat ctggctcaac gagggctggg ccacctacgc gacgtggctg
     3601 tggaacgagg agaacggcat ccgcaccgcc caggccgcct tcgactcctg gtacgccccc
     3661 gcccgcaccc ccgcctactg ggcgctgccg atcgacgacc cgggcccgat gggcctgttc
     3721 gcgggacagg tctacgaccg cggcgccggc accctgcacg ccctgcggct caaggtcggc
     3781 gacgaggcct tcttcgcggg cgtgcagacc tgggttgagc gctacgacga cagcaccgcg
     3841 acgacggccg acttccaggc cgtctacgag gaggtgtccg gccaggacct cgacgggttc
     3901 ttccaggagt ggctcgccac cccggccaag cccagctacc cgtaagccga aggagccggt
     3961 cgcgctcact ggtaggagac gaaccacggt gtcgggctga cctgcgccat cgtctgctcc
     4021 ggcgtgagca tcggcaggtc ctcgtcctcg aagttcttcc acccgagcca caccccatcg
     4081 ggcaggtcgc gcctgaggcg cttccaggtg gactgcttgg caccctgacg gccctgcccg
     4141 tcggcgtgca cgagccactg gatctcgtcc agggtggtgt ccaggcgctc ccggtcggtg
     4201 atcatcgacg gacggaactg gtggagggtg agcaccttgg cgggcaggtc gtgctcgcgc
     4261 accagcgctg ccagccaggc ccccacgcgg ttgacctcgt cgaccccgac cgagccgatc
     4321 tggcgcaggt gcttctggtc gggttccagg cgccattccg ggtcgagggc caacccgacg
     4381 gacgggcggc gcagcagggg ttcgtagaac ttcgcctggg tgaggaagtc ggtgcgcccg
     4441 ggttgcaggt cgatgacgac gtagatgccc gcctgctcgg ccgcctcgac ccaggggagc
     4501 agcacctcga tcggggtccg tctcgagtac gagccgtcct ccccagcggc ctgggacgcc
     4561 acgctggcga tgagctcgaa cgccgggatc acgggttcct tgctgagggc ggtgtactcc
     4621 ttcgccagcg accgtgcgcg gcgaaccgcc tggtccgggg gctgctcacc gagcatcccc
     4681 agggccttgg tctgcggatg tccgtagagc gcgatcatcc ggcgccccgg gaacgggacg
     4741 aagccaccac cgggcagttc caccgcgcga cgcaccgtgc gcacccgctg ggcaacccgc
     4801 tccgcggggc cgaggtcgga ccccagggcg agggtcggcc cgctcccggc gcgcaccggc
     4861 cccatggcat ccggggtcgc ccggacgtcg ccgtgctgca ccgtgacccg gtcggcccct
     4921 gcggcgtcgg ccgtggcagc cacggctggc gggagggcac cgcccggcct gagcagcacg
     4981 acggctccgt gggccttcgg gcgtgggggc aacccgggaa gcgccgcgac cgcgccggca
     5041 tccgacccgt ccacgacgac caggcccgcc gattcgtcaa cgccctcggg cgcgccggca
     5101 ccgacgcgaa ccaccgtgcc cacaccgagc cggtccagct ctgcggccag acccggccca
     5161 gcgacgagca ccgggagtgc gtgggcggtg gccagttcgg ccgcggccgc gacatcggtg
     5221 tcggcactcg ccacgacgac gacaccggcg cggtcgagca ggacacgcga ggcggccaac
     5281 gcctgctcgt ggggggaggc gtcgacgtcg acgacaccgg cacgctggtc ggcggcgacc
     5341 acgagggacg tggagccggc atccgggtcg ccatcatcgc tgccgccgct gctgtccgac
     5401 gtgcaggccg tcgtcgtggc ggcgaccgag ccgacgacga gcacggcggc cgcgcgcaga
     5461 gcggtgcggc gggggagggg ctggttcatt gggggggctc acggtggggg cagggaggcc
     5521 ctaccaccct gacacggctc gccgcggttt cggccgtcgt gcccacccgg gatcggggga
     5581 acccgttctg aggtcacgcc ggacctccgt agactgaggc ggctccaccg caccccaccc
     5641 cgacccgaga aggatccccc gtgctccgca cccacgaggc cggcaccctg cgtgccgccc
     5701 acaccggcca gaccgtcacc ctcaccgggt gggtggcaaa gcggcgtgat cacgggggcg
     5761 tggccttcat cgacctccgc gacgccagcg gggtcgttca ggtcgtggcg cgcgacgagg
     5821 tgttgaccgg tgccgcccac gacctgcgca gcgagtacgt cgtcaaggtc gtcggtgagg
     5881 tcaccgcgcg cgaggaccgc aacatcaacc ccgacctgcc gaccggcgag gtcgaggtcg
     5941 tcgccggcgc catcgaggtg ctcaacgagt cggcgccgct gcccttccag gtcgacgagc
     6001 gcgtcaccgt cggcgaggag gcccggctca agcaccgcta cctcgacctg cgtcgccccg
     6061 gcacccgctc ggccggtcac gccatccggc tgcgctcgaa ggtgagcgct gccgcccgac
     6121 gcgtgctcga ggcccgcgac ttcgtcgaga tcgagacccc gaccctgacc cggtccacgc
     6181 cagagggtgc ccgcgacttc ctcgtgcccg cgcgcctcgc ccccggctcc tggtacgccc
     6241 tgccgcagag cccgcagctg ttcaagcagc tgctcatggt ggccgggatg gagcggtact
     6301 accagatcgc ccgctgctac cgcgacgagg acttccgcgc cgaccgccag cccgagttca
     6361 cccagctcga catcgagatg tccttcgtcg agcaggacga cgtcctcgag ctcggggagg
     6421 ccgtcgtccg cgagatctgg cagctcatcg gcgtcgacct gccgacgccg ttccgccgga
     6481 tgacctacgc cgaggcgatg cgtcgcttcg gctccgacaa gcccgacctg cgcttcggcc
     6541 aggagctcgt cgagtgcacc gactacttcg cggacacccc gttccgggtc ttccaggccg
     6601 agtacgtcgg cgcggtggtc atgcccggcg gtgcgagcca gccgcgcaag cagctcgacg
     6661 cgtggcagga atgggccaag cagcgcggcg cccgcggcct ggcctacgtc ctcgtccagg
     6721 aggacggcac gctcaccggc cccgtggcca agaacctctc cgagaccgag ctcgagggcc
     6781 tcgccgcgca cgtcggcgcg cagcccggtg actgcgtctt cttcgccgcc gggagcaccc
     6841 gctcctcgcg ggccctgctc ggcgccgccc gcctcgagat cggcaagcgc tgcggcctga
     6901 tcgacgagaa cgcctgggag ttcctctggg tcctcgacgc accgctcttc gagccggcgt
     6961 ccgaggcggt cgccgcgggt gacgtcgcgg tcggctcggg cgcgtggacg gccgtccacc
     7021 acgccttcac ctcgccgaag gccgagttcc tcgacacctt cgacaccgac cccgggccgg
     7081 cgctcgccta cgcgtacgac atggtgctca acggcaacga gctgggcggc gggtcgatcc
     7141 gtatccaccg ccgcgacgtg caggagcgcg tcttccaggt catgggcctg tcgcgtgagg
     7201 aggcccagga gaagttcggc ttcctcctcg acgccttcgc gttcggcgcg cccccgcacg
     7261 gcggcatcgc cttcggctgg gaccgcatcg tcatgctgct cggcggcttc gacagcatcc
     7321 gcgacgtcat cgccttcccg aagtcgggcg gcggctacga cccgctcacg gaggcgccgg
     7381 ccccgatcac cccggagcag cgcaaggagg ccgggatcga cagccggccc ccggcatccg
     7441 agccgcctgc ctgacgggtc cgctcagccg aaccagcggc tggtccagcg catgccggtg
     7501 gtgaagccca gccgttcgta gacgcgacgg gccgagtcgt tgtcggcgaa catgccgagg
     7561 gcgcaggccc cggtctgctc gacggcgcgg cgggtgaggt gcgccgtcac ggctgcgccc
     7621 cacccgcggc cgcgctgcga ggcgtcgacg gcgatcccgg cgagcgtggg ggtgccggcc
     7681 gcgcacggct cgctgcaccc ggtggccagc agtgcgccgg tgtgcgggtc ccgtacgccc
     7741 acccagtact ggtccgggcg ggcgaagggc tggccgtggg tgcgcgggct gtgtgcggcg
     7801 aggaaggcct ctgcctcctg cctgctggag gggtccagga cgacgacgtg gtcctcacca
     7861 cggacgggcg cgggggaggt gctggtccac atccagtccc actcaccgcc ccgggtgccg
     7921 aggtcgaggc gcgcctcgac gagcccgacg aggccgcgcg ggacgctgag gtggcggtgg
     7981 ccctcgcccg tgacgaaggc ccgcaccgcc gggtcgtcga gcaggatcgc caggccgccc
     8041 tcggtcgcga gggcggagct gcccgggacc ccgtggtcgg agcgccggtg gaacagcacc
     8101 gaggtctggc cggcgtggtc gacggcgaag gcgggcccga cgaagccggc gccgacgtcg
     8161 aggaccgcga ccggatgccg tgaggtcgcc tcctcgaact cggcgcgcgt cgtgagggtg
     8221 cggaccgccg tcattcggtg attccccatc gttcgtgcag gcgtcgcagc ggtgcggggg
     8281 cccaccagtt ggccgagccg agcaccgaca tcgtggccgg gacgaggagc atgcgcacga
     8341 gggtggcgtc gatgacgatc gccaggacga gggccacccc catctgcttc atgatgagca
     8401 ggtcgcccgc ggcgaagccg gagaagacga tgaccatgag gagcgcggcc gaggtgatga
     8461 tgcgaccgga gcgttgcagg cccagggtca cggcactgcg ggtgtcgtgt ccctgctcgt
     8521 gcagctcgac gatgcgggag agcaggaaga cctcgtagtc catcgacagg ccgaacccga
     8581 aggccagcac gaggagcggg atggtgttct cgagcgcccc ggtcgaggcg aagcgcagca
     8641 acccctcgag gtggccgtcc tcgaagatcc acacggtgac accgagcgcg gccccgaggg
     8701 agagcacgtt catgaccaac gccttgatcg ggatgaccac ggagccggtc atgaggaaga
     8761 gcagcacgag ggtggcgagg acgaccagcc cggcagccca gagcgcccgg tcggccacgg
     8821 tggcgcggaa gtcgacgatg cccgaggcct ggccgacgac gtaggcgtcg aagggggccc
     8881 ggtcgctgcg cagcgagtcg acgaggtcgc gcgaggcctc gcccgtgccg gagtcaccgg
     8941 tctggaagcc gacggtgacg acgccgttgt cgagctcgcg gaccgggtcg acggactcca
     9001 cacccggtcg gtcggcggcg gtgtcggccc aggcctgcac ctcggcgacc ggcgcacggc
     9061 tcacgagcag cacttcggcg ccggcgagca gcgggtagcc cgcccggaag tcctcgtaga
     9121 aggtgcgctc gggggtgccg acgggcagca gctcggggcc ggacgaggtg agctcgaggc
     9181 gtagcgccgg gagggccatg acgacgagca ccgccgagac ggccgcgatg acgacccacg
     9241 gcatccggtg cacgcgctcg gccaggcgcg agaacacgcc gacgtccgag gcggtctccg
     9301 tgccgcgctt gagcaggcgt cgggccccga ggacacacag cgccgggatg agggtcagcg
     9361 cgacggccag ggcgatgagc acgaccgaga caccggccac gctcaccgcg cggatgaacg
     9421 ggatctgcag gacgagcagg ccggacagcg agatggcgac ggtgagggcg gagaagatga
     9481 cggtgcggcc ggcccggttc atcgtcgccg cggtggcggc gaccacctgg gcgtgcgtgg
     9541 cgtcggtggc cggggtgccc tggagcagcg cgcgcatctc ctcgcggaag cgggagacga
     9601 cgagcagccc gtagtcgatg cacaggccca gcccgagcac cgtgacgacg ttgacgaccg
     9661 aggcgtcgag gtcgagcagg tacgacagcc cgaggagcga ggcgagtgcg ccggcgatcg
     9721 aggcgatggc gcccaggacc gggaagccgg cagcgaggaa cccaccgaag acgacgacca
     9781 tgacgaggaa gctgaccgga agggcgatgc cctcaccgcg ctggccgtcg atcttgacct
     9841 gctcgatgat ccggtcgacg agggagcgca gcccaccgcg ttggtccccg gtggcgccgg
     9901 tgtcctcggc gaggccgtcg aacacggcgt cgacctggtc ggtggcggcg gcctcctgct
     9961 cggcggtgtt ggcctcgtcg aaggtgacca ccgtcgcgaa cccgccggat gccgggtcac
    10021 cgtcgagcac gaaccgcagc gcgtcggggg tgctcgggcc gcccggtacg acgaacgggt
    10081 tgacggcgga ttcgacgtgc tcgatggcgg agaggtcctg caccgcggtc gcggcggcgc
    10141 tcgccacggc cgggtcgctg aggtcgaccc cggtgagcgt ctgggtgtag gtgctgaacc
    10201 cgctgccgcc gccctgggcc agcaggtcac ggccgtcctg gttctcgccg gcgacgacga
    10261 tctcaccgct gtcgagccgg ctgaacaggc tcggggtgcc ggtggcgccc agggccaccg
    10321 cgaacccgac gacgatgaag agcacccaga agcccacgat ggccttggcg tgacgggcga
    10381 cgtggcgccc ccaccgctcc agggcgggac tgccgtgccg ggcgctcctg ctcatgcggc
    10441 acagcctgcc aga
//
LOCUS       DAOSOC010000081        10288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_6501_length_10288_cov_9.525750, whole genome shotgun sequence.
ACCESSION   DAOSOC010000081 DAOSOC010000000
VERSION     DAOSOC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6501_length_10288_cov_9.525750"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PL014_07530"
     CDS             <1..1407
                     /locus_tag="PL014_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HOT56977.1"
                     /translation="MFASQVELYNTETVLGGPEGSRDRYDTKHPLYTHVKKLSQVRAA
                     HPALADGAQIARYDTDGSGIFAFSRIGSDTKGKGNASKGKGKGGKDAGRVEYVVAANN
                     GTTPATASFPTWTKGKGAVFTPLFGTDTAVKPSADGTVKVKVPPLSVSVWRANKPIST
                     SGAAPTVEVVLPERGVNGRSEITADVEASTFAQTTFLWRELGAQDWQPLGTDDNAPFR
                     VFHDVSALPLSTPLEYRAVVKDAAGRVAGDSSWTQVVGAPSRTVPENGDPAANQPGTV
                     AIAGTLNSEMGCPADWEPGCDQAQMSLDGTDKIWKKQGVTLPVGPYAYKAALDRAWTE
                     NYGEGGVRDGGNISMETADGTASFYYDPTTHWAVSDEEDPIITVPGSFQSELGCGGDW
                     KPECMRPWLQDKDGDGIYAIITTKIPAGTWEFKVAHGLSFDESYPAANVSVTVPADGA
                     STTFIYNSVTHEVSVTSE"
     gene            complement(1515..2189)
                     /locus_tag="PL014_07535"
     CDS             complement(1515..2189)
                     /locus_tag="PL014_07535"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HOT56978.1"
                     /translation="MKHFLLGVGATLATLALALVVLLLTLGSGDGETIAAPSPTASGP
                     GTGTEPQPPADLTTDETWLGTVDLRGSDIVSADATLTDVVATGSGVRFSESGLRAERL
                     DLDATLPFATVATQIGDGARLYDAGDGLAGIERAVTILGRDLTVRAEGTVVADGGQLL
                     IEPRTVDLGGPDFLDSALSSLARSLVTIRQPVPGVPDGLALTDVTVTPAGFDASLSGT
                     DVTIGR"
     gene            complement(2223..3527)
                     /locus_tag="PL014_07540"
     CDS             complement(2223..3527)
                     /locus_tag="PL014_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT56979.1"
                     /translation="MTAVLTPREAERRLYVLTTTRWLPVGFVVGIIILLQTGRGLTVA
                     QAASSFAIGGLVTFALELPTSGFADAVGRRPVYLTAAVLGVVAAVGIALAQSFWWFVA
                     AAVVTGAFRALDSGPLEAWFVDAVHATTPGADVDRQLSRAGSVLGGAIAVGALASGGL
                     IWWDPVRTVFGVSGVSALDAAAWVSAALTVVHLVAAAALMTEQRPPAAAGRSQWSHAL
                     SEARTTPAVIASGVRLLATNRVLLALVGVEVFWSVGMITFESLMPLRLEELVGSAERA
                     GALVGPVSAAGWGLFAVGSWLAGVTSARIGVARAAMVGRVLNALGAVVMGLVAGPVAL
                     VAAYLFTYSMHGMNGPPHAALLHRQATSANRATVLSINSMMAFLAFAVAAPLVGTLAD
                     RASISTAMVTAGAISALGVACYLPARRQERTRTSLETGAVAR"
     gene            complement(3524..4168)
                     /locus_tag="PL014_07545"
     CDS             complement(3524..4168)
                     /locus_tag="PL014_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT56980.1"
                     /translation="MATSKTPQPAADRTPTLDASDPAAPHTGIRLDATSLRVLAHPLR
                     SRLLSQLRVGGPATATDLAATLGTNSGATSYHLRKLESVGLVADTGEGEGKRRLWRAA
                     TDFHSYYPSDFEGDEDSETALNWLARDYVRHFSEQAERWVDVSPGWPGDWQDACGSSD
                     DMVLLTAPQLQAMRDEISEVVGRYRRVGQGNPDAKRIAVYLFAYALDLDKAPSR"
     gene            4429..4872
                     /gene="rplM"
                     /locus_tag="PL014_07550"
     CDS             4429..4872
                     /gene="rplM"
                     /locus_tag="PL014_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT56981.1"
                     /translation="MRTYTPKQGEITRAWHIIDATDVVLGRLASQTAILLRGKHKPTF
                     APHVDGGDFVIIINAEKVALTGAKLAQKKAYHHSGHPGGLKSTSYTELLAKSPEKAVE
                     KAVRGMLPKNTLGRAQLSHLKVYRGAEHPHAAQQPQPFELTQVAQ"
     gene            4872..5387
                     /gene="rpsI"
                     /locus_tag="PL014_07555"
     CDS             4872..5387
                     /gene="rpsI"
                     /locus_tag="PL014_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT56982.1"
                     /translation="MTENTQIDETAGTDVNDAPVTEYTSESDTNVAGAEPVARPAATV
                     PGAATGRRKQAVARVRIVPGTGEWKINGRTLEGYFPNKVHQQIVNEPLKVLELDGAYD
                     VLVRVHGGGPSGQAGAVRLGVARSLNGVDEELNRPTLKKAGFLTRDARVPERKKAGLK
                     KARKAPQYSKR"
     gene            5514..>6850
                     /gene="glmM"
                     /locus_tag="PL014_07560"
     CDS             5514..>6850
                     /gene="glmM"
                     /locus_tag="PL014_07560"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT56983.1"
                     /translation="MSRLFGTDGVRGLANGPVITAEMALSMSVAAARVLGQAGATPGH
                     RPKAVVGRDPRASGEFLAAAVVAGLASAGVDVYNAGVLPTPAVAYLTADTGADFGVML
                     SASHNAMPDNGIKFFARGGHKLPDDVEDRIEAAMGERWARPIGADVGRVVAMQEGHTR
                     YIAHLLRVLPNQLDGLKVVIDGAHGAASMVSPEIFRLAGAEVIEIGTAPDGLNINDGY
                     GSTHLGPLKAAVVAHGADLGIAHDGDADRCLAVDAAGVEVDGDQIMGILAVDMKERGK
                     LAHDTLVATVMSNLGLLQAMEREGIRVLQTGVGDRYVLEEMRAGGYSLGGEQSGHVIM
                     LDHGTTGDGVLTGLMLAARMAATGRTIADLGTVMTRLPQVLVNVRGVDKNRVESDEGL
                     QDAVRREEAALAGSGRVLLRKSGTEPLVRVMVEAPTQDEADAVAHRLAGVVRER"
     assembly_gap    6851..6950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6951..8339)
                     /locus_tag="PL014_07565"
     CDS             complement(6951..8339)
                     /locus_tag="PL014_07565"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOT56984.1"
                     /translation="MTSFERTFPDGFLWGAATASFQIEGATTEDGRLPSIWDTFCEVE
                     GNVANKDTGDPACDHYHRVDEDVALMSDLGLDVYRFSIAWPRVLPTGTGTVNQPGIDF
                     YDRLVDRLLDHGIRPVATLYHWDLPQALDDRGGWLNRDVAAWFEEYTAVTVGALGDRV
                     KHWTTLNEPWCSSILSYSIGNHAPGHHDTLEGMVAAHHLMLAHGTAVPVIRELCPDGE
                     ASITLNPTYVWGPENGDEHDADAVRRADNALNGLFFGPIFHGAYPDGMIEDTAHLTDH
                     AFIQDGDLETISAPLDNLGVNNYFPTRVRAARDGEDGSNPMPGCDGVIELAPQGPLTA
                     MGWEQSPKSHQLIIERSARESGLPIYITENGSAWEDTVSDDGAVHDPERVNYLHEHLG
                     AVADAIEGGTDVRGYFAWSLLDNFEWAYGYDKRFGIIHVDYDTQKRTIKDSGLEYARI
                     IASEHARTRGDR"
     gene            complement(8351..9190)
                     /locus_tag="PL014_07570"
     CDS             complement(8351..9190)
                     /locus_tag="PL014_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOT56985.1"
                     /translation="MITLVGLAVHPVKSTAVRHVDQAHVEPWGLHDDRRWMVVDPTGE
                     CVTAREHASLLGIQAMTPMTDPGLPVPLRLSAPGAAPLDVEQPHGDPVTVTVHGRSLD
                     AVCAPFVATTWLAATLGIEGLRLVHVHRPRPLNPEYGLPGEATAFADGYPVTLASAAS
                     LRRLDDWVTDGVLERGDVARHPMAVDRFRPNLVVDGDLEAFEEDHWRSVLIGDVSFRV
                     VKAVDRCVLTTIDPTTLERGHEPIRTLARHRRRDGRTWFAMHLVPQVRGVVRVGDEVT
                     PGR"
     gene            complement(9213..9875)
                     /locus_tag="PL014_07575"
     CDS             complement(9213..9875)
                     /locus_tag="PL014_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOT56986.1"
                     /translation="MTTARGNPRPRTPHDAWAELVAGNNRFVDGASDHPHQSIQLREE
                     LATGQTPFATVFGCSDSRVAAEIIFDQGLGDVFVVRTAGTVIDPGVLGSLEFGVEVLG
                     TPLIVVLSHTSCGAIRATLEAVATRQLPGGFLRDVVERIAPSYLLAGSTAEPTPGEVG
                     RAHLRHVVSLLAERSRAIRYAVERGDLAIVGAEYGLAGGAVEIVTVIGDLGVPAGPPG
                     TA"
     gene            complement(9891..>10288)
                     /locus_tag="PL014_07580"
     CDS             complement(9891..>10288)
                     /locus_tag="PL014_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOT56987.1"
                     /translation="VTCMDSRIDPLRMIGLKPGDAKIFRNPGGRVTDQALEALVLATH
                     LLGVNRVLVIPHTRCAMANSTEAELQRRVGESAGRDATWHRFNTIDDQEAALRSDVVR
                     VKSHPLVADEVLVGGFIYDVDTGLLTQVD"
BASE COUNT         1566 a         3527 c         3685 g         1410 t
ORIGIN      
        1 atgttcgcca gccaggtcga gctctacaac accgagaccg tgctcggtgg ccccgaggga
       61 tcgagggacc gctacgacac caagcacccc ctctacaccc acgtgaagaa gctctcccag
      121 gtcagggctg cgcacccggc gctcgcggac ggtgcccaga tcgctcggta cgacaccgac
      181 ggctccggca tcttcgcgtt ctcccggatc ggctcggaca ccaagggcaa gggcaacgca
      241 agcaagggga agggcaaggg aggcaaggat gccggtcgcg tcgagtacgt cgtcgccgcg
      301 aacaacggca ccacgcccgc cacggcatcc ttcccgacct ggaccaaggg caagggggcg
      361 gtgttcaccc cgctgttcgg caccgacacg gcggtcaagc cgtccgctga cgggacggtc
      421 aaggtcaagg tgccgccgct cagcgtcagc gtgtggcgtg ccaacaagcc gatcagcacc
      481 tccggcgcgg ccccgaccgt cgaggtcgtc ctccccgagc gtggtgtcaa cggacggtca
      541 gagatcacgg ccgacgtcga ggcgagcacc ttcgcgcaga cgacgttcct gtggcgcgag
      601 ctcggcgcgc aggactggca gccgctgggc accgacgaca acgctccgtt ccgcgtcttc
      661 cacgacgtgt ccgcactgcc gctgtccacg ccgctggagt accgggccgt cgtcaaggat
      721 gccgcgggcc gggttgccgg tgactcgtcc tggacgcagg tcgtcggtgc gccctcgcgc
      781 acggtccccg agaacggtga cccggcagcg aaccagcccg gcaccgtcgc gatcgccggg
      841 acgctgaact ccgagatggg gtgcccggcc gactgggagc cggggtgcga ccaggcccag
      901 atgagcctgg acggcacgga caagatctgg aagaagcagg gggtcaccct gccggtcggc
      961 ccgtacgcgt acaaggccgc tctcgaccgc gcctggaccg agaactacgg cgagggtggc
     1021 gtgcgcgacg gcggcaacat ctcgatggag acggccgacg gcacggcgtc gttctactac
     1081 gacccgacca cccactgggc cgtctcggac gaggaggacc cgatcatcac cgtgcccggg
     1141 tcgttccagt cagagctcgg ctgcggcggg gactggaagc cggagtgcat gcggccgtgg
     1201 ctgcaggaca aggacggcga cggcatctac gcgatcatca cgacgaagat cccggcgggc
     1261 acgtgggagt tcaaggtggc gcacgggttg tccttcgacg agagctaccc cgcggccaac
     1321 gtgagcgtga cggtcccggc ggacggtgct tcgacgacgt tcatctacaa ctcggtgacc
     1381 cacgaggtga gtgtcacgag cgagtgacgt gtacccacat cgaccaatag gtcactcggg
     1441 gtacatggct ggccgtcccc gcagtgacct attggtcgat tccgggacgg tgcacgtggt
     1501 tgggcggctg tgactcagcg gccgatcgtg acgtcggtgc ccgagagtga ggcgtcgaac
     1561 ccggccggtg tgacagtcac gtcggtgagg gccagcccgt ccggcacacc cggcacgggc
     1621 tggcggatcg tcacgaggct gcgcgccaac gaactgaggg cgctgtcgag gaagtccggc
     1681 ccgccgaggt cgacggtgcg gggctcgatg agcagttgcc cgccatcggc gaccacggtt
     1741 ccctcagccc ggaccgtgag gtcccggccg aggatggtca ccgcgcgttc gatgcccgcg
     1801 agcccgtcac cggcatcgta gagccgtgcc ccgtcgccga tctgggtggc gaccgtggcg
     1861 aacggcaggg tggcatcgag gtcgagccgc tcggcgcgca ggccgctctc ggagaatcga
     1921 accccggatc cggtggccac cacgtcggtg agcgtcgcat cggctgacac gatgtcggag
     1981 ccgcgcaggt cgacggtgcc gagccacgtc tcgtcggtgg tgaggtcggc gggcggctgc
     2041 ggctcagtgc ccgtccccgg gccggatgcc gtcgggctcg gtgccgcgat cgtctcgccg
     2101 tcgccggacc cgagggtgag gagcaggacg acgagggcga gggcgagcgt ggccagggtc
     2161 gcgccgaccc cgagcaggaa gtgcttcatg cccccagcgt gacaggccga cgatgccgta
     2221 cgtcagcgcg ccaccgcacc ggtctccagc gacgtcctgg tgcgctcctg ccgtcgggcc
     2281 gggagatagc acgccacgcc gagcgcgctg atcgcgcccg cggtgaccat ggccgtgctg
     2341 atcgacgccc ggtcggcgag ggtgccgacg agcggagccg ccacggcgaa ggcgaggaag
     2401 gccatcatcg agttgatcga cagcaccgtc gcgcggttgg cggacgtcgc ctgccgatgg
     2461 agcagcgccg cgtggggcgg gccgttcatc ccgtgcatgg agtaggtgaa caggtaggcc
     2521 gcgacgagcg cgaccgggcc ggccaccaac cccatgacca cggccccgag ggcattgagc
     2581 acgcgcccga ccatggccgc tcgggcgacg ccgatgcgtg ccgacgtcac cccggcgagc
     2641 cacgagccca cggcgaacag cccccacccg gcggccgaca ccggcccgac cagggccccc
     2701 gcccgctccg cggacccgac gagctcctcc aggcgcaacg gcatcaggga ctcgaaggtg
     2761 atcatgccga ccgaccagaa cacctcgacg ccgaccaggg ccagtagcac gcggttggtg
     2821 gccagcagcc gcaccccgct cgcgatgacc gcgggcgtgg tgcgtgcctc ggacagggcg
     2881 tgcgaccact gggagcgacc agccgccgcg ggcgggcgct gctcggtcat cagcgctgcg
     2941 gccgcaacga ggtgcaccac cgtcagggcc gcgctgaccc acgccgcggc atccagtgcg
     3001 gagacacccg agacgccgaa gaccgttcgc acgggatccc accagatgag cccaccggac
     3061 gcgagtgcgc ccacggcgat ggccccaccc agcaccgagc ccgcccggga gagctgccgg
     3121 tcgacgtcgg ccccgggggt cgtggcgtgg acggcgtcga cgaaccacgc ctcgagcggc
     3181 ccggagtcca gggcgcggaa cgccccggtg accaccgcgg ccgccacgaa ccaccagaac
     3241 gactgggcga gcgcgatgcc cacggcggcg acgaccccga ggaccgcggc ggtgaggtac
     3301 accggccgcc gccccacggc atccgcgaaa ccactcgtcg gcagctcgag ggcgaaggtc
     3361 acgagaccgc cgatggcgaa ggacgacgcc gcctgggcga cggtcagccc gcgcccggtc
     3421 tgaagcagga tgatgatgcc gacgacgaac ccgacgggca gccagcgggt cgtcgtcagg
     3481 acgtagagcc ggcgctcggc ctcgcgagga gtgaggactg cagtcacctc gacggtgcct
     3541 tgtcgaggtc cagcgcgtag gcgaacaggt agaccgcgat ccgcttggcg tcggggttgc
     3601 cctggccgac gcgccggtag cggccgacga cctcgctgat ctcgtcacgc atcgcctgga
     3661 gctggggggc ggtgagcagc accatgtcgt cgctgctgcc gcaggcgtcc tgccagtcgc
     3721 ccggccagcc gggggagacg tcgacccagc gctcggcctg ctcgctgaag tgccgcacgt
     3781 agtcgcgggc gagccagttc agcgccgtct cggagtcctc gtcaccctcg aagtcgctcg
     3841 ggtagtagct gtggaagtcg gtggcggccc gccacagccg gcgcttgccc tcgccctcgc
     3901 cggtgtcggc gaccaggccc accgactcca gcttgcgcag gtggtaggac gtggcgcccg
     3961 agttggtgcc gagggtggcg gcgaggtcgg tggcggttgc cggcccgccg acccgcagct
     4021 gggagagcag gcgggagcgc agggggtggg ccaggacgcg caggctcgtg gcgtcgaggc
     4081 ggatgccggt gtgcggtgcc gcggggtcgc tcgcgtcgag cgtgggggtg cggtccgcgg
     4141 ctggttgcgg ggtctttgac gtggccatgc gtgcacaata cctttgcata ctctgtttgc
     4201 acaactactc tgcacaattg gtgtgcgccc gcgtttgtgc aggtcagcga cctgccagcc
     4261 gggttcggcg acgatttggg gtgttgccca accacccgta aacttgacca ctgttgtgcc
     4321 gtgcgccctc gaccggggca cgtgaggttc cctggtggga gcacgcggca tacgccagtt
     4381 ccatcggggc gatcctcgcc cctgcccctt ctacgaccag gacacctgat gcgtacctac
     4441 acccccaagc agggcgagat cacgcgtgcg tggcacatca tcgacgcgac cgacgtcgtg
     4501 ctcggccgcc tcgccagcca gaccgcgatc ctgctgcgcg gcaagcacaa gcccaccttc
     4561 gccccccacg tcgacggtgg cgacttcgtc atcatcatca acgccgagaa ggtggccctc
     4621 acgggcgcca agctcgcgca gaagaaggcc taccaccact cgggtcaccc gggcggcctg
     4681 aagagcacgt cctacaccga gctgctcgcc aagagcccgg agaaggccgt cgagaaggcc
     4741 gttcgcggca tgctccccaa gaacaccctc ggtcgcgcgc agctgagcca cctcaaggtc
     4801 taccgcggcg ccgagcaccc ccacgctgcc cagcagcccc agcccttcga gctcacccag
     4861 gtggcccagt gatgaccgag aacacccaga tcgacgagac cgccggtacc gacgtcaacg
     4921 acgccccggt caccgagtac acctccgagt cggacaccaa cgtcgcgggt gccgagcccg
     4981 tggctcgtcc ggcggccacc gttcccggcg cggccaccgg tcgtcgcaag caggccgtcg
     5041 cccgcgtccg gatcgtcccg ggcaccggcg agtggaagat caacgggcgc accctcgagg
     5101 ggtacttccc gaacaaggtc caccagcaga tcgtcaacga gccgctgaag gtcctcgagc
     5161 tcgacggcgc ctacgacgtc ctcgtgcgcg tgcacggcgg tggcccctcc ggccaggccg
     5221 gcgcggtccg cctcggtgtc gcgcgctccc tcaacggggt cgatgaggag ctcaaccgcc
     5281 cgacgctgaa gaaggccggc ttcctcaccc gggacgcccg cgtccccgag cgcaagaagg
     5341 ccggtctcaa gaaggcccgc aaggcgcctc agtacagcaa gcgctgatcc ggcgcgacgg
     5401 catccgatcg tcggtgccgt cccagctccg aaggcggcac cattcggtgc cgccttcggc
     5461 gcgttcggga tagattcagg tcagggctcc atcggcgaaa ggcaccatcc agcatgtctc
     5521 gactcttcgg caccgacgga gtgcgcggcc tggccaacgg cccggtcatc accgccgaga
     5581 tggccctgtc gatgtcggtc gcggccgccc gcgtcctcgg ccaggccgga gcgacgccgg
     5641 ggcaccggcc caaggccgtc gtcgggcgcg acccgcgggc ctcgggggag ttcctcgccg
     5701 cggcggtcgt cgcgggtctg gcctcggccg gggtcgacgt ctacaacgcc ggggtcctgc
     5761 cgacccccgc cgtggcctac ctgaccgccg acaccggcgc cgacttcggg gtgatgctct
     5821 cggcgtcgca caacgcgatg cccgacaacg gcatcaagtt cttcgcccgc ggtggccaca
     5881 agctccccga cgacgtcgag gaccggatcg aggccgcgat gggcgagcgc tgggcgaggc
     5941 cgatcggggc tgacgtcggc cgggtcgtgg ccatgcagga gggccacacc cgctacatcg
     6001 cccacctcct gcgcgtgctg ccgaaccagc tcgacggtct caaggtcgtc atcgacggcg
     6061 cccacggggc ggccagcatg gtctccccgg agatcttccg tctggcgggc gccgaggtca
     6121 tcgagatcgg caccgccccc gacgggctga acatcaacga cggctacggc tcgacccacc
     6181 tcggcccgct caaggccgcc gtggtcgcgc acggtgcgga cctcggcatc gcccacgacg
     6241 gggatgccga ccggtgcctt gcggtggatg ctgccggcgt cgaagtcgac ggcgaccaga
     6301 tcatgggcat cctggcggtg gacatgaagg agcgcggcaa gctcgcgcac gacaccctcg
     6361 tcgcgaccgt catgagcaac ctcgggctgc tccaggcgat ggagcgcgaa ggcatccggg
     6421 tgctccagac cggcgtcggt gaccgctacg tgctggagga gatgcgcgcc ggcggctact
     6481 ccctcggcgg cgagcagtcc gggcacgtca tcatgctcga ccacggcacc accggtgacg
     6541 gggtgctcac ggggctcatg ctcgcggccc ggatggctgc caccggacgc accatcgccg
     6601 acctcggcac ggtcatgaca cgcctgcccc aggtgctcgt caacgtccgg ggcgtcgaca
     6661 agaaccgggt cgagagcgac gaaggcctcc aggacgccgt gcgtcgcgaa gaggccgccc
     6721 tcgcgggctc cgggcgggtg ctcctgcgca agtcaggcac cgaaccgctc gtgcgcgtca
     6781 tggtcgaggc accgacccag gacgaggcgg atgccgtggc gcaccgcctc gccggcgtcg
     6841 tgcgcgagcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcagcggtcg
     6961 ccgcgggtgc gagcgtgctc gctcgcgatg atccgcgcgt actccagccc ggagtccttg
     7021 atcgtgcgct tctgcgtgtc gtagtcgacg tggatgatcc cgaagcgctt gtcgtagccg
     7081 taggcccact cgaagttgtc gagcagcgac cacgcgaagt acccgcgcac gtcggtgccg
     7141 ccctcgatgg cgtcggcgac cgcgccgagg tgctcgtgca ggtagttgac ccgctcgggg
     7201 tcgtgcaccg cgccgtcatc ggacacggtg tcctcccagg cggagccgtt ctcggtgatg
     7261 tagatcggca ggccgctctc gcgcgcggac cgctcgatga tcaactggtg gctcttgggc
     7321 gactgctccc atcccatcgc ggtgagcggg ccctgcggcg cgagttcgat gacgccgtcg
     7381 cagccgggca tggggttgct gccgtcctcg ccgtcgcggg cggcgcgcac ccgggtgggg
     7441 aagtagttgt tgaccccgag gttgtccagc ggtgccgaga tcgtctcgag gtcaccgtcc
     7501 tggatgaagg cgtggtcggt gaggtgagct gtgtcctcga tcatgccgtc ggggtaggcg
     7561 ccgtggaaga tcgggccaaa gaacagaccg ttgagggcgt tgtcggcgcg ccgcacggca
     7621 tccgcatcat gctcgtcacc gttctcgggg ccccacacgt acgtcgggtt gagggtgatc
     7681 gacgcctccc cgtcggggca cagctcgcgg atcaccggca cggccgtgcc gtgcgcgagc
     7741 atcaggtggt gcgcggcgac catgccctcg agggtgtcgt ggtggccggg ggcgtggttg
     7801 ccgatgctgt agctgaggat cgaggagcac cacggctcgt tgagggtcgt ccagtgcttg
     7861 acccggtcgc cgagggcgcc gacggtgacg gcggtgtact cctcgaacca ggcggcgaca
     7921 tcgcggttga gccacccacc gcggtcgtcg agggcctgcg gcaggtccca gtggtagagc
     7981 gtcgcgacgg gacggatgcc gtggtcgagc agtcggtcga cgagccggtc gtagaagtcg
     8041 atgccgggct ggttgaccgt gccggtgccg gtgggcagga cgcgcggcca ggcgatggag
     8101 aagcggtaga cgtcgagccc gaggtcgctc atgagtgcga cgtcctcgtc gacgcggtgg
     8161 tagtggtcgc aggccgggtc gccggtgtcc ttgttcgcga cgttgccctc gacctcgcag
     8221 aacgtgtccc agatcgacgg cagtctcccg tcctccgtgg tcgccccctc gatctggaag
     8281 ctggcggtgg cggcccccca caggaagccg tcggggaagg tgcgctcgaa ggacgtcata
     8341 acgggtgaga ttaccgtccc ggtgtgacct cgtcgccgac tctgaccacc ccgcggacct
     8401 gcgggacgag gtgcatcgcg aaccacgtcc tgccgtcgcg gcgacgatgg cgcgccaggg
     8461 tgcggatcgg ctcgtgcccg cgctccagcg tcgtggggtc gatcgtcgtg aggacacagc
     8521 ggtcgaccgc cttgaccacc cggaacgaga cgtccccgat gagcacgctg cgccagtggt
     8581 cctcctcgaa cgcctcgaga tcgccgtcga cgacgaggtt gggacggaag cggtcgacgg
     8641 ccatcggatg ccgtgccacg tctccgcgct cgaggacgcc gtcggtgacc cagtcgtcga
     8701 ggcggcgcag ggatgccgcg gacgccaggg tgaccgggta gccgtcggcg aaggcggtgg
     8761 cctcgccggg caggccgtac tcggggttga gggggcgcgg gcggtgcacg tggaccaggc
     8821 gcagcccctc gatgccgagc gtggcggcga gccaggtggt ggccacgaac ggtgcgcaga
     8881 cggcatccag gctgcgcccg tgcacggtca ccgtgaccgg gtcgccgtgc ggctgctcga
     8941 cgtcgagcgg cgcggcaccg ggcgcgctga gccgtagggg gacgggcagg ccggggtcgg
     9001 tcatgggggt catggcctgg atgcccagca gggatgcgtg ctccctcgcg gtgacgcact
     9061 cgccggtggg gtcgacgacc atccaacgac ggtcgtcgtg caggccccag ggctcgacgt
     9121 gtgcctggtc gacgtggcgg actgcggtgc tcttgacggg gtgaacggca agcccgacca
     9181 gggtgatcac gtgggctcgc tgccgggcgt gctcacgccg tccccggtgg tccagcaggc
     9241 acaccgaggt cgccgatgac cgtcacgatc tcaacggccc cgccggcgag cccgtactcc
     9301 gcgccgacga ttgccaggtc accgcgttcg accgcgtacc ggatggcccg ggagcgctcg
     9361 gcgagcaggc tgacgacgtg ccgcaggtgg gcgcggccga cctcaccggg cgtcggctcc
     9421 gcggtgctgc cggccagcag gtagctcggg gcgatgcgct cgaccacgtc gcgaaggaac
     9481 cccccgggca gctggcgggt cgccaccgct tcgagggtgg cccggatcgc cccgcacgag
     9541 gtgtgggaca gcacgacgat gagtggggtg ccgagcacct cgacgccgaa ctcgagcgag
     9601 ccgagcaccc cggggtcgat gacggtgccc gccgtgcgca ccacgaagac gtcgcccagg
     9661 ccttggtcga agatgatctc cgcggcgacg cgggagtccg agcagccgaa caccgtggcg
     9721 aagggggtct ggccggtcgc gagctcctcg cgcagctgga tgctctggtg cgggtggtca
     9781 ctcgcaccgt cgacgaagcg gttgttgccg gcgacgagtt cagcccacgc gtcgtgcggg
     9841 gtccgcggcc tggggtttcc tcgagcggtc gtcatcgcga cgtcgagcgc tcagtcgacc
     9901 tgggtgagca ggcccgtgtc gacgtcgtag atgaacccac cgacgagcac ctcgtcggcg
     9961 acgagcgggt gcgacttcac ccggacgacg tccgagcgca gcgccgcctc ctggtcgtcg
    10021 atggtgttga agcggtgcca ggtcgcgtcg cggccggctg attcacccac ccgccgctgg
    10081 agttcggcct cggtcgagtt cgccatcgcg cagcgggtgt gcgggatgac gagcacccgg
    10141 ttcaccccga gcaggtgcgt ggcgagcacc aatgcctcga gggcctggtc ggtgacccgg
    10201 ccaccgggat tgcggaagat cttcgcgtcg ccgggcttga gcccgatcat ccgcagcggg
    10261 tcgatccggc tgtccatgca ggtgacga
//
LOCUS       DAOSOC010000082        10169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_6601_length_10169_cov_9.636346, whole genome shotgun sequence.
ACCESSION   DAOSOC010000082 DAOSOC010000000
VERSION     DAOSOC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6601_length_10169_cov_9.636346"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1562
                     /locus_tag="PL014_07585"
     CDS             18..1562
                     /locus_tag="PL014_07585"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOT56988.1"
                     /translation="MDESRPEVMLADVVEASRAVAATRSRTAKTQALADLLTRCAPEE
                     VGTVVSHLSGVLPQRRLGVGFRSLRDLPEPAQTPTLTVLEVDAAFERLASAHGTGSTA
                     SRRSELSTLMSRATSVEQQFLRDLMTGNLRQGALDGVVLAAISAAYAVPAATVRRAAM
                     LAGTAAEVATLAMTGGSRALEDVSLVVGRPVRPMLAASAKTTGEAAESIGESELIVDG
                     KLDGIRVQVHRRGGEVTVYTRSLDEITDRVPEVVEAVAALPGGDLVVDGEAIVLRADG
                     RPEPFQVTGARTASSADLTGLQARMPLTTFLFDVLHVGGRDLLDDSARVRHEVLLDLA
                     PHLLVPRLLTDDPAAAEQFFADLVAAGHEGVVIKDADSPYAAGRRGSGWVKVKPVHTL
                     DLVVLAVEWGSGRRVGSLSNIHLGARDPATGEFTMVGKTFKGMTDEVLAWQTKRFAEL
                     AVDGTDGWVVPVRPVQVVEIAVDGVQRSSRYPGGVALRFARVLRYRDDKEADAADTID
                     TVRGLL"
     gene            complement(1606..2748)
                     /gene="nagA"
                     /locus_tag="PL014_07590"
     CDS             complement(1606..2748)
                     /gene="nagA"
                     /locus_tag="PL014_07590"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOT56989.1"
                     /translation="MILTAATVLTGEYSHSPGWVEVDHGVISAVGSGVPPRPADVDLG
                     DVVLTPGFVDMHVHGGGGGAYFEATDTAVAASVTLHRRFGTTSTMASLVSDHHDVLVA
                     QVRALADHVESGELLGIHLEGPWLSPHRCGAHEPSALRDPDPAEVSALLEAGRGTIRM
                     VTLAPELPGATEAIRRIVDGGAVAAIGHTEATYEQARAAIDAGATVATHLFNAMRPVH
                     HREPGPVVAALEDPRVTVELVTDGVHLHPALHRLVTTDVGPDRVALVTDAMAAAGMQD
                     GSYRLGSLAVDVVDQIARVAGTDTIAGSTATMQRLVAQTVNHGGADRAESLRTTIRQV
                     TVNPLRALGLPAPGLSPGAPADLVVLSPDLDVVQVMRAGTWVTLPG"
     gene            complement(2752..3537)
                     /gene="nagB"
                     /locus_tag="PL014_07595"
     CDS             complement(2752..3537)
                     /gene="nagB"
                     /locus_tag="PL014_07595"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOT56990.1"
                     /translation="MEVVILDDARDLASVAADAITALLARKPDAVLGLATGSSPLAVY
                     DELTARHERGEVSFAQARAFTLDEYVGLPAGHPERYRTVIDRDFVTRVDFAPDAVQGP
                     DGLADDIPASCAAYEQAIADAGGVDLQILGIGSVGHIAFNEPGSSFASRTRIKTLTQQ
                     TRLDNARFFDGDVSQVPTHCLTQGLGTIMAARHVVLLATGASKAEAVHQLVEGPVSAL
                     WPATILQHHPHATVLLDPAAAGRLQLAGYYRETFAGKPTWQGI"
     gene            complement(3539..>4117)
                     /locus_tag="PL014_07600"
     CDS             complement(3539..>4117)
                     /locus_tag="PL014_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HOT56991.1"
                     /translation="SAPASAAAPATPATPAAAPAAPTAPAAPTAPGARVAVLSPIAGT
                     VVDLADVPDATFAKGLVGPGLAIDPPREVVDAVAPIAGTIVKLWPHAYAIQSPEGVGV
                     LVHLGIDTVQLDGAGFDLLAAQGDTVELGQPILRYDVAAVEAAGRNPVVPVIVLERKA
                     HDVTLRGLEAGDQVRDLELLLTASATKTKVGG"
     assembly_gap    4118..4217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4218..5783)
                     /locus_tag="PL014_07605"
     CDS             complement(<4218..5783)
                     /locus_tag="PL014_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOT56992.1"
                     /translation="MSTVVDTNPPLEKPAEKQGLRIPGFAQLQRLGKSLMLPIALLPA
                     AGILLRLGQEDLLGQYDWPVLGPFFDAMSAAGGAIFANLPLLFAVGVAIGFARKADGS
                     TALSAVAGYLVISEVFKAMSPVVLEGETTPGGDQAMINYSVFAGILVGLVTAYLFDRF
                     HTIQLPPYLGFFGGRRFVPIVVSFSSLFMGFALSYFYPIFNAGLTGLAEFIAGAGAFG
                     AFVYGFVNRMLIPLGLHHIVNTYIWFLYGDYTVGTEVYTGELTRFAQGDPTAGRLTSG
                     FYPILMFGLPAAALAMIHAANKNQKKVAIGILSAAGLTAFLTGITEPLEFAFMFVAFP
                     LYVLHAVLTGLSLAIAYLLDIHLGFSFSAGLIDLLLYGTAPAAKNIPLLIVMGVVFFA
                     VYYVVFRFAITKWNLMTPGRDPKGATDEDLEAATSAAAGAGTGAATDRAQQLITAFGG
                     ADNLKNVDACITRLRIEVGDKSLVDQGALKALGAAGVIEVGNNVQAIFGTQADGLKEE
                     IRAALAGGSAPASA"
     gene            5925..6800
                     /locus_tag="PL014_07610"
     CDS             5925..6800
                     /locus_tag="PL014_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT56993.1"
                     /translation="MLTRTLGPFTVSAIGLGAMPMSMGAPDQPAEDRAVATVHAALDA
                     GVTFIDTADIYAPSWDQMGHNERLVAKALASYGDTSGIIVGTKGGITRGPGEAWGRDG
                     SPEYIRSAVEASLRALDRDVIDLYQWHRPDRWRLYSEVVETFRALQDEGKVRAVGISN
                     ANVEEIEVAIEVLGEGGLASVQNEFSPRFRSSRDELEFCGERGIAFLPWSPLGGTGAH
                     SKEVGQAYPAFHEIGLAHGVSPQQVVLAWELSLGEHVIPIPGASRPESITDSAQAAHL
                     ELTDAELEAIEAQFC"
     gene            6887..7621
                     /locus_tag="PL014_07615"
     CDS             6887..7621
                     /locus_tag="PL014_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT56994.1"
                     /translation="MKVPKYYRVKREILALIADLPTGAAVPTEREFAEQFDTSRTTVR
                     QAIAELVIEGRLERTQGRGTFVSQPKLMQVRQATSFSQDLTAEGWSPGSRILAVTVEQ
                     ATDAMVGPLRVEPGQPVQRVERLRTRGDEVIAHEVAHLPGVFPGLAEHLAATGSLYRT
                     LAEVYGRVVDAVEDSVETTLADPVAADLLGVDTGLPLLLVHRTAWDAQGQPVEWTRSL
                     FRGDRFRFVARHRLDDVKHPAAAESH"
     gene            7703..8212
                     /locus_tag="PL014_07620"
     CDS             7703..8212
                     /locus_tag="PL014_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT56995.1"
                     /translation="MTIEIPRFLTKNQADLVRETEPHRMEALDEDTLLDLHTRVRRAR
                     SKYVKLYRQTGAQRVGMKGARGHARPGNAANGAKAEVFELALARVSARLEVVARAAAE
                     ELKNERLAAAAAVRSSTGPGTTKGAGGADGSAKASTRRRATKTTGGLKRDASTKSKGK
                     SRQAKKDAR"
     gene            8284..>8556
                     /locus_tag="PL014_07625"
     CDS             8284..>8556
                     /locus_tag="PL014_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOT56996.1"
                     /translation="MTRHPVMSATSRGLWTFTADTDLLLRLSASGFDWLAIDAQHGPV
                     DRAALHAIGRALADAATPLVVRVPAVDAAWIGAALDAGAAAVIVPSI"
     assembly_gap    8557..8656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8913..9611)
                     /locus_tag="PL014_07630"
     CDS             complement(8913..9611)
                     /locus_tag="PL014_07630"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOT56997.1"
                     /translation="MTPQTFPILATADESWTSYIPLIWQALGETLYMVVFAAALTFVL
                     GMLIGIALYVTSPGGIRPLRWINVPLGLYVNISRSLPFLILIIVLIPVTRFIVGTSLG
                     ATAAIVPLTFGTAPFFGRLVETALREVDKGKVEAAEAMGATHGDIVRKVLLPEALPAL
                     LAGTTLTLVMFVDYSAMAGAIGAGGLGSFAIKYGYQRFNTELMLVTVAILVLIVQAIQ
                     LGGEAVVRRVARRR"
     gene            complement(9608..>10169)
                     /locus_tag="PL014_07635"
     CDS             complement(9608..>10169)
                     /locus_tag="PL014_07635"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HOT56998.1"
                     /translation="QRVGIARALAGSPRVLLCDEATSALDSRTAQQILALLRDVNEAF
                     GVTIVLITHDMEVVRSACGSAALLEDGRIVEAGRLTDLSLDAGSALGRLLLPVRVAPV
                     GADETHLLLTVADVAHGSAALSQLARDLDVDVAIRAGTIEQIAGDDVGRFQVALDPGS
                     GRSGPVATDAVVRYLTDRNIRVEVLA"
BASE COUNT         1501 a         3373 c         3713 g         1382 t
ORIGIN      
        1 cgaggcagga tggccggatg gacgagtcgc gccccgaggt catgctcgct gacgtcgtgg
       61 aggcctcccg ggcggtggcc gccacgcgct cgcgcacggc caagacgcag gcactggcag
      121 acctgctgac ccggtgcgcc ccggaagagg tggggacggt ggtgtcgcac ctgtccggcg
      181 tgctgcccca gcgccgcctc ggcgtggggt tccgttccct gcgtgacctc cccgagccgg
      241 cgcagacccc gacgctcacg gtgctcgagg tcgatgccgc gttcgagcgc ctggcgagcg
      301 cccacggaac cggctccacg gcatcccgcc gcagtgaact gagcaccctg atgagccgcg
      361 ccaccagcgt cgaacaacag ttcctgcgcg acctcatgac cggaaacctg cgccagggcg
      421 cgctcgacgg cgtggtgctc gccgccatct cggcggccta cgccgtcccg gccgccacgg
      481 tccgccgcgc tgccatgctc gccggcaccg ccgccgaggt cgcgaccctc gccatgacgg
      541 gagggtcgcg cgctctcgag gacgtctcgc tcgtcgtggg gcgtcccgtg cgaccgatgc
      601 tcgcggcatc cgccaagacc accggcgaag cggccgagag catcggcgag agcgaactga
      661 tcgtcgacgg caagctcgat ggcatccgcg tccaggtgca ccgccgtggc ggtgaggtga
      721 cggtctacac gcgcagcctc gacgagatca ccgaccgcgt gcccgaggtc gtcgaggccg
      781 tcgcggccct gccgggtggc gacctcgtcg tcgacggcga ggccatcgtg ctgcgggccg
      841 acggcaggcc ggagcccttt caggtcaccg gtgcgcgcac ggcgagctcc gccgacctca
      901 ccggtctcca ggcccggatg ccgctcacga cgttcctctt cgacgtcctc cacgtgggtg
      961 gtcgcgacct gctcgacgac agcgcccggg tgcgtcacga ggtgctgctc gacctcgcgc
     1021 cgcacctgct ggtcccacgg ctgctgaccg acgaccccgc cgccgcggag cagttcttcg
     1081 ccgacctcgt cgccgccggg cacgaaggtg tcgtcatcaa ggacgccgac agcccgtatg
     1141 ccgccgggcg gcgaggcagc gggtgggtca aggtcaagcc ggtgcacacg ctggacctcg
     1201 tggtgctcgc ggtcgagtgg ggatcagggc ggcgggtggg cagtctgtcg aacatccacc
     1261 tcggggcgcg cgacccggcg accggcgagt tcacgatggt gggcaagacg ttcaagggca
     1321 tgaccgacga ggtgctggcg tggcagacca agcgcttcgc cgagctcgcc gtcgacggca
     1381 ccgacgggtg ggtggttccg gtgaggccgg tgcaggtcgt cgagatcgcc gtcgacgggg
     1441 tgcaacgctc cagtcgctac ccggggggcg tggcgctgcg gttcgcccgg gtgctgcgct
     1501 atcgcgacga caaggaggca gatgcggccg acacgatcga caccgttcgc ggcttgctgt
     1561 gacgtgtgcc cggcgatggg tcggtagcgc cgcggatggg tgagctcacc cgggcagggt
     1621 gacccacgtc ccggcgcgca tcacctgcac gacgtccaga tcgggtgaga gcacgacgag
     1681 gtcggccggg gcgccggggg agagccccgg cgccgggagg ccgagggcgc gcaacgggtt
     1741 gacggtgacc tgacggatgg tggtccgcag ggactcggcg cggtccgcgc ccccgtggtt
     1801 caccgtctgc gccacgaggc gctgcatcgt cgcggtgctg ccggcgatcg tgtccgtgcc
     1861 agccacgcga gcgatctggt cgacgacgtc gacggccagc gaccccagcc ggtaggagcc
     1921 gtcctgcatc ccggcggcag ccatggcgtc ggtcaccagg gccacccggt cagggccgac
     1981 gtccgtggtg accagccggt gcagcgccgg gtgcaggtgc accccatccg tgacgagttc
     2041 aaccgtgacc ctggggtcct cgagggcggc gacgaccggg ccgggttcgc ggtggtgcac
     2101 cgggcgcatc gcgttgaaca ggtgggtggc gaccgtggcc ccggcatcga tcgcggccct
     2161 ggcctgctcg tacgtcgcct cggtgtgccc gatggcggcc actgccccgc cgtcgacgat
     2221 gcggcggatc gcctcggtcg cgccgggcag ctcgggcgcc agggtcacca tccggatcgt
     2281 gccccgcccg gcctcgagca gcgccgacac ctcggccggg tcggggtcgc gcagggcgct
     2341 cggttcgtgg gcgccgcaac ggtgcggtga caaccacggg ccctcgaggt ggatgccgag
     2401 cagctcaccc gactcgacgt ggtccgcgag agccctgacc tgcgcgacga gcacgtcatg
     2461 gtggtcggag acgagggagg ccatcgtcga cgtggtcccg aagcggcggt gcaacgtcac
     2521 ggatgccgcg accgccgtgt cggtcgcctc gaagtaggcc ccaccgccac caccgtgcac
     2581 gtgcatgtcg acgaagccgg gtgtcagcac gacatcaccc aggtcgacgt cggccggtcg
     2641 cggagggacg ccggatccga cagccgagat gacgccgtgg tcgacctcga cccatcccgg
     2701 ggagtgggag tactccccgg tgagcacggt ggcagcggtg aggatcatcg atcagattcc
     2761 ttgccaggtg ggtttgcccg cgaaggtctc gcggtagtag cccgcgagct gcagccgacc
     2821 ggcggcggcc gggtcgagca gcacggtcgc gtgcgggtgg tgctgcagga tcgtcgccgg
     2881 ccacagggcg ctgaccgggc cctcgacgag ctggtgcacc gcctcggcct tcgacgcgcc
     2941 ggtcgccagc aggacgacgt gccgggccgc catgatcgtg ccgagaccct gggtgaggca
     3001 gtgggtcggc acctggctga cgtcgccgtc gaagaagcgc gcgttgtcca gccgggtctg
     3061 ctgggtcaac gtcttgatgc gcgtccgtga ggcgaacgac gatcccggct cgttgaaggc
     3121 gatgtgcccg accgagccga tgcccaggat ctggaggtcg acgccaccgg catccgcgat
     3181 ggcctgctcg taggcggcgc acgatgccgg gatgtcgtcg gcgagcccgt cggggccctg
     3241 gacggcatcc ggggcgaagt cgacccgggt gacgaagtcg cggtcgatca ccgtgcggta
     3301 gcgctcgggg tgcccggccg ggaggcccac gtactcatcg agcgtgaacg cccgggcctg
     3361 cgcgaagctc acctcgccac gctcgtggcg cgccgtcagc tcgtcgtaca cggcgagcgg
     3421 tgacgacccg gtggcaagcc cgagcacggc atccggcttc ctggcgagca gcgccgtgat
     3481 ggcgtccgcg gcgaccgacg cgaggtcgcg tgcgtcgtcg aggatcacga cctccacgtc
     3541 agccaccgac cttggtcttg gtggcggatg ccgtgaggag gagctcgagg tcgcggacct
     3601 ggtcgccggc ctcgaggcca cgcagggtga cgtcgtgggc cttgcgctcg aggacgatca
     3661 cgggcacgac cgggttgcgg ccggcggcct cgaccgcggc gacgtcgtag cgcaggatcg
     3721 gctggccgag ctcgacggtg tcgccctggg ctgcgaggag gtcgaagccc gcaccgtcga
     3781 gctgcaccgt gtcgatgccc aggtgcacga ggacaccgac gccctcgggc gactggatgg
     3841 cgtaggcgtg cggccacagc ttgacgatgg tgccggcgat cggcgccacg gcgtcgacga
     3901 cctcgcgggg cgggtcgatg gccaggccgg ggccgacgag acccttggcg aaggtggcgt
     3961 cggggacgtc cgccaggtcg accacggtgc cggcgatcgg cgagagcacg gcgacgcggg
     4021 ccccgggggc cgtcggagcg gcaggtgcgg tcggtgctgc cggtgcagct gcgggggtgg
     4081 cgggggtggc gggtgcagcg gcggatgccg gggcggannn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnggc ggatgccggg gcggagccac ccgcgagggc cgcgcggatc
     4261 tcctccttga ggccgtctgc ctgggtgccg aagatggcct ggacgttgtt gccgacctcg
     4321 atgaccccag cggcgccgag ggccttcagg gcgccctggt cgacgaggga cttgtcgccg
     4381 acctcgatgc gcaggcgggt gatgcaggcg tcgacgttct tgaggttgtc ggctccaccg
     4441 aaggcggtga tgagctgctg tgccctgtcg gtggctgcgc cggtgccagc gccggcggct
     4501 gcggacgtgg cggcctcgag gtcttcgtcg gttgcgccct tcgggtcgcg gccgggggtc
     4561 atgaggttcc acttggtgat cgcgaagcgg aacaccacgt agtagacggc gaagaagacc
     4621 acgcccatga cgatgagcaa cgggatgttc ttggctgcgg gtgcggtgcc gtagagcagc
     4681 aggtcgatca ggccggcgga gaacgagaac ccgaggtgga tgtcgagcag gtaggcgatg
     4741 gccagggaca ggccggtgag cacggcgtgc aggacgtaca gcgggaacgc gacgaacatg
     4801 aaggcgaact cgaggggctc ggtgatgccg gtgaggaagg cggtcaggcc cgcggccgac
     4861 aggatgccga tggcgacctt cttctggttc ttgttcgcgg cgtggatcat cgccagcgca
     4921 gcggcgggca ggccgaacat gaggatcggg tagaagccgg aggtgaggcg gccggccgtg
     4981 gggtcgccct gggcgaagcg ggtgagctca ccggtgtaga cctcggtgcc gacggtgtag
     5041 tccccgtaga ggaaccagat gtaggtgttg acgatgtggt gcaggccgag ggggatgagc
     5101 atgcggttga cgaagccgta gacgaaggcg ccgaacgcgc ccgccccggc gatgaactcg
     5161 gcgaggccgg tcagacccgc gttgaagatc gggtagaagt agctgagggc gaagcccatg
     5221 aacagcgagg agaacgacac gacgatcggc acgaagcggc ggccgccgaa gaagccgagg
     5281 tagggcggca gctggatcgt gtggaagcgg tcgaacaggt aggcggtcac gaggccgacg
     5341 aggatgccgg cgaagacgct gtagttgatc atcgcctggt cgccgccggg ggtggtctca
     5401 ccctcgagca ccaccgggga catcgccttg aagacctcgg agatgacgag gtagccggcg
     5461 acggccgaca gggcggtcga gccgtcggcc ttgcgggcga agccgatggc cacaccgacg
     5521 gcgaagagca gggggaggtt ggcgaagatc gcaccacccg cggcgctcat ggcgtcgaag
     5581 aagggcccga ggacgggcca gtcgtactgg ccgagcaggt cctcctggcc caggcgcagc
     5641 aggatgccgg ccgcgggcag cagggcgatg ggaagcatga ggctcttgcc gagccgctgg
     5701 agctgggcga agccggggat gcgcagcccc tgcttctccg ctggcttctc cagcggcggg
     5761 ttggtgtcga cgaccgtgct catggtgccc tccgaatggc gtgaattggt gtagaccaag
     5821 ttcagattgg catagaccaa tcgtcgtcgt caagggaggc tgagaacttt ctcgcgacgt
     5881 cggcccggcc tgccgtgtcg gtgcgccgcc ataggctggt cgacatgttg acccgcaccc
     5941 tcggaccgtt caccgtctcc gccatcggcc tcggcgccat gccgatgtcg atgggagccc
     6001 ccgaccagcc agccgaggac cgggcggtcg ccaccgtgca cgccgccctc gacgccgggg
     6061 tgacgttcat cgacaccgcc gacatctacg ccccctcgtg ggaccagatg gggcacaacg
     6121 agcgcctggt cgcgaaggcc ttggcgtcct acggcgacac gagcggcatc atcgtcggga
     6181 ccaagggcgg gatcacccgc ggcccggggg aggcctgggg tcgcgacggc tcgccggagt
     6241 acatccgcag cgcggtcgag gcgtcgctgc gggctcttga ccgcgacgtc atcgacctct
     6301 accagtggca ccgacccgac cgctggcggc tctacagcga ggtcgtcgag acgttcaggg
     6361 ccctccagga cgaaggcaag gtccgcgccg tcggcatctc caacgccaac gtcgaggaga
     6421 tcgaggtcgc gatcgaggtg ctcggcgagg gtggtctggc cagtgtccag aacgagttct
     6481 ccccgcgctt ccggtccagc cgcgacgagc tcgagttctg cggggaacgc ggcatcgcct
     6541 tcctgccgtg gagcccgctc ggtggcaccg gagcccacag caaggaggtg ggtcaggcct
     6601 accccgcctt ccacgagatc ggcctcgcgc acggggtgag cccgcagcag gtcgtgctcg
     6661 cgtgggagct gtcgctcggc gagcacgtca ttccgatccc gggcgcgagc cgacccgagt
     6721 cgatcaccga ctcggcgcag gcggcgcacc tcgagctcac cgacgccgag ctcgaggcca
     6781 tcgaggcgca gttctgctga gccgcctcag gccggccccc ggcatggcgt agattggtgt
     6841 agaccaccct cgcaggggtg tcggccactc ggcgtcaaga ggtgcgatga aggtcccgaa
     6901 gtactaccgc gtcaagcgcg agatcctcgc tctcatcgcc gacctgccca cgggggcggc
     6961 cgtcccgacc gagcgcgagt tcgccgagca gttcgacacc tcccgcacca ccgtgcgcca
     7021 ggccatcgcc gaactcgtca tcgagggccg tctcgaacgc acccaggggc gcggaacctt
     7081 cgtctcgcaa cccaagctca tgcaggtgcg ccaggcgacc tcgttcagcc aggacctcac
     7141 cgccgagggc tggagcccgg gcagtcgcat cctcgcggtc accgtcgagc aggccaccga
     7201 cgccatggtc gggcccttgc gggtcgagcc ggggcagccc gtccagcggg tcgaacggct
     7261 gcgcacccgc ggcgacgagg tcatcgccca cgaggtggcc caccttcccg gagtcttccc
     7321 cggtctggcc gagcacctgg ccgccacggg gtcgctctac cgcaccctcg cggaggtgta
     7381 cggccgcgtc gtggatgccg tcgaggacag tgtcgagacc acgctcgccg acccggtggc
     7441 cgccgacctc ctcggcgtcg acaccggtct gccgctcctg ctcgtgcacc gcacggcctg
     7501 ggacgcgcag ggccaaccgg tcgagtggac caggtcgctg tttcgtgggg accgcttccg
     7561 gttcgtggcc cgccaccggc tcgacgacgt gaagcaccct gctgcggccg agtctcattg
     7621 acagccgagg ctcattgaca ctggccgtgc gcggagcaag gctgccggta tcggccgaat
     7681 ccaccctcga gaggacccgt ccatgaccat cgagattccc cgcttcctca ccaagaacca
     7741 ggccgatctc gtgcgcgaga ccgagccgca ccggatggag gcgctcgatg aggacaccct
     7801 gctcgatctg cacacccggg tccggcgagc ccgctcgaag tacgtgaagc tctaccggca
     7861 gaccggtgcc cagcgggtgg gcatgaaggg ggcccgtggc cacgcccggc cgggcaatgc
     7921 ggcgaacggc gccaaggccg aggtgttcga actggccctg gcccgggtca gtgcccgcct
     7981 cgaggtcgtc gcccgcgccg ccgccgagga gctcaagaat gagcggctgg ccgccgccgc
     8041 cgcagtgcgc agctcgacag gcccgggcac cacgaagggt gccggagggg ccgacggttc
     8101 ggccaaggcc agcacccggc gccgcgccac gaagaccacc ggtggtctca agcgcgacgc
     8161 ctcgacgaag tccaagggca agagccgtca ggcgaagaag gacgcgcgct gacctcagcg
     8221 tcggggcgtg acgtcggcgc ccccgacggc gctcatgtcg acgctggcac cggcgggcac
     8281 gcggtgaccc gacacccggt catgtccgcg acgtcgcgag gcctgtggac cttcaccgcc
     8341 gacaccgacc tgctcctgcg gctcagcgcc agcggcttcg actggctcgc catcgacgcc
     8401 cagcacgggc cggtcgaccg cgccgcactc cacgcgatcg gccgggccct ggcggatgcc
     8461 gcgaccccct tggtcgtgcg cgtgcccgcc gttgatgcgg cgtggatcgg ggccgccctc
     8521 gatgccggtg cggccgccgt catcgtgccg tcgatcnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnncctc acgacgtggg gctggatcca cctgctgctc gggctgctcg
     8701 tgctcgcggc cggagccggc ctgttcttcg gtgcgacgtg agcgcgcacg gtcgcggtca
     8761 tcgcggcctg cgtcagcatg ctcggcaact tcgcgtggct gccgaactac ccggtgtggg
     8821 ggctgaccct catcgcgctg gacgtgttca tcatctgggc cgtcatcgtg cacgggcggg
     8881 ccttcgtcga cgagtgaccc ccggcctggt ggtcagcgtc gccgggcgac gcgacgcacc
     8941 accgcttcac cgccgagctg gatcgcctgg acgatgagga cgaggatggc cacggtgacc
     9001 agcatgagct cggtgttgaa gcgctggtag ccgtacttga tggcgaacga gccgagcccg
     9061 cccgcgccga tggccccggc catggccgag tagtcgacga acatcaccag ggtgagcgtg
     9121 gtcccggcga gcagtgcggg cagggcctcg ggcagcagca ccttgcgcac gatgtcgccg
     9181 tgggtggccc ccatggcttc ggccgcctcg accttgccct tgtcgacctc gcgcagcgcg
     9241 gtctcgacca gccgcccgaa gaacggcgcg gtgccgaagg tcagcggcac gatcgccgcg
     9301 gtggccccga gcgaggttcc gacgatgaag cgcgtgaccg ggatgagcac gatgatcagg
     9361 atgaggaacg gcagcgagcg gctgatgttg acgtagagac cgagcgggac gttgatccac
     9421 cgcaacggcc ggatgccgcc cggtgacgtc acgtacaacg cgatcccgat gagcatcccc
     9481 agcacgaacg tcagggctgc cgcgaagacc accatgtaga gggtctcgcc gagggcctgc
     9541 cagatcaggg ggatgtagct ggtccacgac tcgtcagcgg tcgccaggat cggaaaggtc
     9601 tggggcgtca tgcgaggacc tccacgcgga tgttgcggtc agtgaggtag cgcaccaccg
     9661 cgtcggtggc gacgggcccg ctgcgaccgg agccggggtc cagggccacc tggaagcggc
     9721 ccacgtcgtc accggcgatc tgctcgatcg tcccggcgcg gatggcgacg tcgacgtcga
     9781 ggtcgcgggc cagctggctc agtgccgctg acccgtgggc gacgtcggcc accgtgagca
     9841 gcaggtgggt ctcgtcggcg ccgacggggg cgacgcgcac gggcagcaag aggcgcccca
     9901 gggccgaccc ggcgtcgagg ctgaggtcgg tgagccgacc cgcctcgacg atgcgtccgt
     9961 cctcgagcag ggcagccgag ccgcacgctg agcgcaccac ctccatgtcg tgggtgatga
    10021 gcacgatggt cacgccgaac gcctcgttca cgtcgcgcag cagggcaagg atctgctgcg
    10081 ccgtacggga gtcgagggcg ctcgtcgcct cgtcgcagag cagcacgcgc ggtgacccgg
    10141 cgagcgcccg ggcgatgccg acgcgctgc
//
LOCUS       DAOSOC010000083        10115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_6650_length_10115_cov_8.256163, whole genome shotgun sequence.
ACCESSION   DAOSOC010000083 DAOSOC010000000
VERSION     DAOSOC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6650_length_10115_cov_8.256163"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PL014_07640"
     CDS             complement(<1..386)
                     /locus_tag="PL014_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOT56999.1"
                     /translation="MDRQQEFVLRTIEERDIRFVRLWFTDVLGTLKSVAIAPAELEGA
                     FAEGIGFDGSVIEGFARIYEADMLAKPDPSTFQVLPWRGESPGTARMFCDLTLPDGTP
                     SMADPRHVLQRALTKAADLGFTFYTHP"
     gene            501..1412
                     /gene="panB"
                     /locus_tag="PL014_07645"
     CDS             501..1412
                     /gene="panB"
                     /locus_tag="PL014_07645"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOT57000.1"
                     /translation="MTESTPTSPDAVASPAGTPPPAPEGTAPYGTGAQAAPQRRIRVP
                     HLHQMKERGEKWAMLTAYDMYTAEIFDEAGIPVLLVGDSAGNNVYGFETTVPVTVDHL
                     VPLTRAVTSAAKRAMVVADLPFGSYQASPQQALATATRFMKEGLAHAVKLEGGAAMVP
                     EVELLVRAGIPVMGHIGFTPQSEHVLGGYKVQGRGAGADKLMDDALALEAAGCFAVVI
                     EMVPSPAAEAVTKALRIPVVGIGAGPHCDAQVLVWQDMAGLRGGRAPRFVKKYADLRT
                     ALSDAATAYAADVAGGTFPASEHSFES"
     gene            1457..2101
                     /locus_tag="PL014_07650"
     CDS             1457..2101
                     /locus_tag="PL014_07650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT57001.1"
                     /translation="MWPLDTPNAPSPTRWSALQSGPEAAAEYRKRFLDLEEAGTDIHG
                     EARFVTTLVPPPSRVLDAGCGFGRVASKLTRLGHTAIGVDADEHLIELAREDADTRFF
                     VADLSTLNLRAEQEFHCVVMAGNVVPYLADGTLAAVLERLSSHLAPGGYLVAGFGLSG
                     SALPENAAPVSLRVYDRLAEAAGLSFINRYATWDREPFVAGGSYAVSVHRRTGG"
     gene            complement(2108..2314)
                     /locus_tag="PL014_07655"
     CDS             complement(2108..2314)
                     /locus_tag="PL014_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminopeptidase"
                     /protein_id="HOT57002.1"
                     /translation="MKYWYNVDTGQVETDDTRSQDATVMGPYDTHDAAAAALETARAN
                     TERWDAEDRKWSGGGSWDEESQED"
     gene            2357..3229
                     /gene="map"
                     /locus_tag="PL014_07660"
     CDS             2357..3229
                     /gene="map"
                     /locus_tag="PL014_07660"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT57003.1"
                     /translation="MPLTTAPVAPGALSPRRPVPASIARPEYVDQPAPAKFTGSEVKD
                     AETIEAMRVAGRIAANAMAAAAAVIAPGVTTDEIDRVGHEYLLDHGAYPSTLGYRGFP
                     KSLCTSVNEVICHGIPDSRPLEDGDIVNIDITAYIGGVHGDTDATYLVGDVDEESRLL
                     VERTHEAMMRGIRAAVPGRAVNVIGRVIESYAKRFGYGVVRDFTGHGIGSAFHSGLII
                     PHYDAAPAYDTIIEAGMTFTVEPMLNLGTAEWEMWDDRWTVVTKDRRRSAQFEHTILV
                     TDHGPEILTQGDAA"
     gene            3226..4005
                     /locus_tag="PL014_07665"
     CDS             3226..4005
                     /locus_tag="PL014_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT57004.1"
                     /translation="MSTALPLGIDVGGSGIKGAPVDLDKGEFAQERVRIDTPAESTPE
                     AVCDIIARIVEQFDEVHDGSPIGVTVPGVVQHGVVKTAANIHKSWINCEIEKLLEDRL
                     QHDVVVVNDADAAGVAELAYGAAKGQDGLVIMTTLGTGIGTAVINRGVLVPNSELGHL
                     EIDGVDAETVAASSIKTAEGLSYEEWALRLQRYYRYLEDLMWPDLIVVGGGVSKKSDK
                     FLPLLDLRTPIVPAVLRNAAGIIGAAVLANGYQQERGRHTS"
     gene            complement(4117..4776)
                     /locus_tag="PL014_07670"
     CDS             complement(4117..4776)
                     /locus_tag="PL014_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT57005.1"
                     /translation="MRITHLGHACLLVEMADVRVLIDPGAFVDDFSEAHDLDAVIVTH
                     RHPDHLDPQRLPGILADNPNAAVLCDPESVAAFADLGVEAREHGGTTTRIGGVTITPF
                     GEQHALIHDDLPRITNVGVRLDAEGEPSFFHPGDALDAVPGQVDVLAFPLQAPWQRSR
                     DMTAFLRRFGAPHAVPIHDGLLQRRGRDLYLGQAAQLGCADTRIHDLAGAGPVEFREG
                     A"
     gene            complement(4815..5462)
                     /locus_tag="PL014_07675"
     CDS             complement(4815..5462)
                     /locus_tag="PL014_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HOT57006.1"
                     /translation="MARQHPLDPGVARRSVMAAVRELDGVGVRDALLECLLAHGVDVA
                     VAEVVMPVLREVGEGWERGEMGVLHEHFVSSSFRGVLGELRRPLPAQLSRTVVLACPP
                     HELHDLPLELFGAMLHARSWRVVSLGANTPMTAVADAVRILDADACVLAGVRRSVFES
                     RMPSLTRLGRRAPVFLAGQGAVRLPEPPPMTVVLPPDLRAAADLVDEVARESAAG"
     assembly_gap    5537..5636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5748..6140)
                     /locus_tag="PL014_07680"
     CDS             complement(5748..6140)
                     /locus_tag="PL014_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOT57007.1"
                     /translation="MRPHLLDANVLIALVVADHEHHDRASRWLTQVKGFAVCPVVEGA
                     LVRFLLRIGESATTASHLVQQVNSHPSCQFWGDELSYGHVDLSDVRGHRQVTDVYLAA
                     LAASRGSRLATFDTALTRLRPESTVLVP"
     gene            complement(6137..6367)
                     /locus_tag="PL014_07685"
     CDS             complement(6137..6367)
                     /locus_tag="PL014_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57008.1"
                     /translation="MRTTVDLPPAVHRRATELAVRTGRSLSAVLADLTVRGLAQIEEP
                     VVLGTDDRTGLPVLAVGRRVTSAEVADLLDDE"
     gene            complement(6423..6803)
                     /gene="rnpB"
                     /locus_tag="PL014_07690"
     ncRNA           complement(6423..6803)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PL014_07690"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6846..8303)
                     /locus_tag="PL014_07695"
     CDS             complement(6846..8303)
                     /locus_tag="PL014_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOT57009.1"
                     /translation="MPHPRVRLTPTGADIPDGVLADLRDRFAAVRADLDLSLAYPAEA
                     LAEAERVAAQPPTLPERDETDLPFITLDPPGSMDLDQAMHLERDSDGYRVRYAIADVP
                     AFVRLGGALDAETRERGQSVYCPDLRVPLHPEVLSEGAGSLLPDAVRPAFVWDLHLDA
                     AGVLGAAEVYRAMVRSVQRLAYDEVQEAIDAGSDDERFVLLREIGERRIERERVRGGA
                     SLPMPEQQIVERPDGGFTIEFRPPVPAEEWNAQISLLTGMAAASMMLEAGVGVLRTMP
                     PPAAGAVAQYRRVARALGAHWPQSMPYGEFIRSLDRTDPRHLALIHEASGLFRGAGYT
                     AFDGELPAQRDHAAVAAPYAHVTAPLRRLVDRFGLMVCEAVCRGDEVPDAVRAALPDL
                     AAHMRSSDHRARSADRACADATEAAVLVGREGELFDAVIVDRTDKGVVVQLVDLPVLT
                     RSRGSGVELGEHVSVRLVRADVAAGALEFEIAGEG"
     gene            8337..9083
                     /locus_tag="PL014_07700"
     CDS             8337..9083
                     /locus_tag="PL014_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOT57010.1"
                     /translation="MLILLPPSESKTNRHRGKPVDPASWSFPDLNPVRARVAQALAAV
                     SASPDAPALLDVSPGLLDDIARNLVLDSAPSTPAAEVYTGVLYDALGLASLDTAARRR
                     ANRWIVVVSALYGAVRPTDRIAAYRLAMGTTLPDVGALAATWRPALEQVLPPLVGRGV
                     IVDCRSAPYAAAWRPGGDLTHRWVQIRVPGASHMAKHTRGLVARHLCEVGCDVRSVPA
                     LADVVGEAFTVALHEPASARAPWVLDVSAP"
     gene            9056..10045
                     /locus_tag="PL014_07705"
     CDS             9056..10045
                     /locus_tag="PL014_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOT57011.1"
                     /translation="MGARRVGALITEAIGALERLVPIVVFLLAITVVAEIAERAGVFD
                     VAGHWVAHAGRHRAWALWVLFALANTGSLLLPVSNLTNLLALHSFQRQGLGHVDYLAL
                     AWAPALACVVVTLLLLVVLHRRTLAARYEVDPPADPHDPVLLRWSAVVCIALGPLFAV
                     GAPPWAVSLVAAALLLSVGLWRAPDLLRGLPVPWLMAAGFVAVAVSVEVLHRHGLDAV
                     VDRVVPSGTGATALLAVAGLGAGLANVVNNLPAYLIIEPAVADSSTRLMALLVGVNAG
                     PLVTPWASLATLLWLARCRSVGVAIPWRWLVPAGAACAVLSVSAGTVALAFVT"
BASE COUNT         1397 a         3653 c         3526 g         1439 t
ORIGIN      
        1 gggtgcgtgt agaacgtgaa cccgaggtcg gctgccttgg tcagcgcccg ctgcaggacg
       61 tgccgggggt cggccatgct gggggtgccg tcgggcaggg tcaggtcgca gaacatccgc
      121 gcggtcccgg ggctctcgcc gcgccacggc agcacctgga aggtgctggg gtcgggcttg
      181 gccagcatgt ccgcctcgta gatcctggcg aagccctcga tgaccgagcc gtcgaagccg
      241 attccctcgg cgaacgctcc ctcgagctcg gccggggcga tggccaccga cttcagcgtg
      301 ccgagcacgt cggtgaacca gagccggacg aaccggatgt cgcgttcttc gatggtgcgg
      361 aggacgaact cctgctgacg gtccatgggc cgaatcctgc cgcacccgtg tgtcgcgcgg
      421 gtaacaggtc ggcgcgcgtc cggcatccga acatctgcga cccccgcgtc aggcgaggcg
      481 ccgcggcata cgatcggccc atgaccgagt cgacgccgac cagcccggat gccgtggcct
      541 cccccgcggg caccccgccc cccgctccgg agggcacggc gccgtacggc acgggtgccc
      601 aggcggcgcc gcagcggcgc atccgggtgc cgcacctgca ccagatgaag gagcggggcg
      661 agaagtgggc gatgctcacc gcctacgaca tgtacaccgc cgagatcttc gacgaggccg
      721 gcatccccgt gctgctcgtc ggtgacagcg ccggcaacaa cgtctacggc ttcgagacca
      781 ccgtccccgt gaccgtcgac cacctcgtgc cgctgacccg ggcggtgacg agcgcggcca
      841 agcgggccat ggtcgtcgcc gacctgccgt tcggctccta ccaggccagc ccgcagcagg
      901 ccctggcgac cgcgacccgc ttcatgaagg aaggcctggc ccacgccgtc aagctcgagg
      961 gcggggcggc gatggtgccc gaggtcgagc tgctcgtgcg cgccggcatc cccgtcatgg
     1021 gtcacatcgg cttcaccccc cagagcgagc acgtgctcgg cgggtacaag gtgcagggtc
     1081 gcggggccgg ggccgacaag ctcatggacg acgccttggc gctggaggcc gccggctgct
     1141 tcgccgtcgt catcgagatg gtgccctccc ctgcggccga ggccgtcacg aaggcgctgc
     1201 gcatccccgt cgtcggcatc ggcgccggtc cgcactgcga cgcccaggtg ctcgtgtggc
     1261 aggacatggc cgggctgcgc ggcggacggg ccccaaggtt cgtgaagaag tacgccgacc
     1321 tgcgcacggc gctctccgac gcggccacgg cgtatgccgc cgacgtcgcc ggaggcacgt
     1381 tcccggcatc cgagcactcc ttcgagtcct gaaccacgac accgacaccg gccgcgacag
     1441 agagcgtcta cccaccatgt ggccccttga cacgccgaac gccccgtccc cgacgcgctg
     1501 gtcagcgctg cagagcgggc ccgaggcagc cgccgagtac cgcaagcgct tcctcgacct
     1561 cgaggaggcc ggcaccgaca tccatggcga ggcgcgcttc gtcaccacct tggtgcctcc
     1621 gccgtcacgg gtgctggatg ccgggtgcgg cttcgggcgc gtggcgagca agttgacccg
     1681 cctcgggcac acggcgatcg gggtcgacgc cgacgagcac ctgatcgagc tcgcccgaga
     1741 ggacgccgac acgcggttct tcgtcgccga cctgtcgacc ctgaacctgc gcgccgagca
     1801 ggagttccac tgcgtggtga tggcgggcaa cgtcgtgccc tacctcgccg acggcacgct
     1861 ggccgcggtg ctcgagcggc tgtcgtcaca cctcgcgccc ggtggctacc tcgtggcggg
     1921 tttcgggctg tcggggtcgg cactgcccga gaacgccgcg ccggtgagcc tgcgcgtcta
     1981 cgaccggctc gcggaggccg ccgggttgtc gttcatcaac cggtacgcga cgtgggaccg
     2041 cgagccgttc gtggccggtg ggtcgtatgc cgtgtcggtg caccgccgca ccggcggctg
     2101 aacggggtca gtcctcctgg gactcctcgt cccacgaacc gccacctgac cacttgcggt
     2161 cctcggcgtc ccagcgctcg gtgttggccc gcgccgtctc cagcgcagca gccgcggcgt
     2221 cgtgggtgtc gtaggggccc atgaccgtgg cgtcctggct gcgggtgtcg tcggtctcga
     2281 cctgaccggt gtccacgttg taccagtact tcatcgccat cctccttggt ggtgggcttt
     2341 gcctagactc caccgtatgc cgttgacgac cgctcccgtg gcccctggtg cactctcacc
     2401 tcgacgaccc gttccggcga gcatcgcgcg acccgagtac gtcgaccagc cggcccccgc
     2461 gaagttcacc ggctcggagg tcaaggacgc cgagaccatc gaggcgatgc gcgtcgccgg
     2521 tcgaatcgcc gccaacgcga tggctgcggc ggccgccgtc atcgccccgg gagtgaccac
     2581 cgacgagatc gaccgcgtgg gtcacgagta cctgctcgac cacggtgcct acccctcgac
     2641 cctgggctac cgcggcttcc ccaagtcgct gtgcacgtcg gtcaacgagg tgatctgcca
     2701 cggcatcccc gacagccgcc cgctcgagga cggcgacatc gtcaacatcg acatcaccgc
     2761 ctacatcggt ggcgtgcacg gcgacaccga cgccacgtac ctcgtgggcg acgtcgacga
     2821 ggagtcgcgc ctgctcgtcg agcgcaccca cgaggcgatg atgcgcggca tccgggccgc
     2881 cgttccgggc cgggcggtca acgtcatcgg ccgggtcatc gagtcgtacg ccaagcgctt
     2941 cggctacggc gtcgttcgcg acttcaccgg gcacggcatc gggtcggcct tccacagcgg
     3001 cctgatcatc ccgcactacg acgcggcacc cgcctacgac accatcatcg aggcggggat
     3061 gaccttcacc gtcgagccca tgctcaacct cggcaccgcc gagtgggaga tgtgggacga
     3121 caggtggacg gtggtgacga aggaccgccg acgttcggca cagttcgagc acacgatcct
     3181 cgtcaccgac cacggtccag agatcctcac ccagggagac gcagcatgag cacagcattg
     3241 cccctcggca tcgacgtcgg cggaagcggc atcaagggcg ctcccgtcga cctggacaag
     3301 ggcgagttcg cgcaggagcg ggtgcggatc gacacccccg ccgagtccac gcccgaagcg
     3361 gtgtgcgaca tcatcgcgcg gatcgtcgag cagttcgacg aggtgcacga cggttccccg
     3421 atcggggtga cggtgcccgg cgtcgtgcag cacggtgtcg tgaagaccgc cgcgaacatc
     3481 cacaagtcgt ggatcaactg cgagatcgag aagctcctcg aggaccgcct ccagcacgac
     3541 gtcgtcgtcg tcaacgacgc ggatgccgct ggcgtcgccg agctcgcgta cggcgccgcc
     3601 aagggccagg acggcctcgt catcatgacg acgctcggca ccgggatcgg gaccgcggtg
     3661 atcaaccggg gtgtgctcgt gcccaactcc gagctgggcc acctcgagat cgacggggtc
     3721 gacgccgaga cggtggcagc cagctcgatc aagacggctg agggcctcag ctacgaggag
     3781 tgggctctcc gcctgcagcg ctactaccgc tacctcgaag acctcatgtg gcccgacctc
     3841 atcgtcgtcg gtggtggcgt gtcgaagaag tcggacaagt tcctgccgct cctcgacctg
     3901 cgcaccccca tcgtgccggc ggtgctgcgc aacgccgcgg gcatcatcgg cgcggccgtg
     3961 ctcgcgaacg gctaccagca ggaacgcggt cgccacacca gctgagccgc ggagctgcgc
     4021 gccctgagcc cgggggctgc gcgaacgtgg gtgagaaccg tcgtctgagc gacgatcttc
     4081 acccacattc ggatgccggg tggccggcat ccctcgtcag gcgccttcgc ggaactccac
     4141 ggggccggca ccggcgaggt cgtggatgcg ggtgtcggcg cagccgagct gcgccgcctg
     4201 ccccaggtag aggtcgcgac cccgtcgctg gagcaacccg tcgtggatgg ggacggcgtg
     4261 cggcgcgccg aaccggcgca ggaaggccgt catgtcacgg ctgcgctgcc acggtgcctg
     4321 gagcgggaag gcgagcacgt cgacctgacc cggaacggca tccagcgcgt cgcccgggtg
     4381 gaagaacgac ggctcgccct cggcgtcgag acgcacgccg acgttggtga tgcgcggcag
     4441 gtcgtcgtgg atcagcgcgt gctgctcacc gaacggggtg atcgtgacgc caccgatccg
     4501 ggtcgtggtg ccaccgtgct cgcgcgcctc gacgccgagg tcggcgaacg ccgccacgct
     4561 ctcggggtcg cagagcaccg ccgcgttcgg gttgtcggcg aggatgccgg gtagccgctg
     4621 cgggtcgagg tgatcggggt ggcgatgggt cacgatcacg gcgtcgaggt cgtgtgcctc
     4681 gctgaagtcg tcgacgaagg cgcccgggtc gatgaggacg cgcacgtccg ccatctcgac
     4741 gagcaggcag gcgtgtccga ggtgggtgat ccgcatgtcc ccacggtagg gcgtgccggg
     4801 cggccggggc agcctcagcc ggccgccgac tcgcgggcga cctcgtcgac gaggtccgcg
     4861 gcggcacgca ggtcgggggg cagcaccacc gtcatgggcg gcggttccgg caggcgcacc
     4921 gccccctggc cggcgaggaa gacgggggcg cggcgaccga gccgggtgag cgacggcatc
     4981 cgggactcga agaccgagcg gcgcaccccg gcgagcacgc acgcgtcggc gtcgagaatg
     5041 cggaccgcgt cggcgacggc ggtcatcggg gtgttggcgc cgaggctgac cacccgccag
     5101 gaccgggcgt gcagcatcgc cccgaacagc tccagaggca gatcgtgcag ctcgtgcggc
     5161 gggcaggcca gcacgacggt gcgcgacagc tgtgccggca gcggccgccg cagctccccg
     5221 agcacccccc ggaacgagct ggagacgaag tgctcgtgca gcacccccat ctcgccgcgc
     5281 tcccagccct cgccgacctc gcgcagcacc ggcatcacga cctcggcaac ggcgacgtcg
     5341 accccgtgcg cgagcaggca ctccaggagt gcgtcccgca cgccgacccc gtcgagctcg
     5401 cgcaccgcag ccatgaccga gcggcgtgcc acgccggggt cgagcgggtg ctgccgggcc
     5461 atctgcgtcc tctccgggcc gagatcggcc cggaaaacgg tacggcacgc gagggcctgc
     5521 gcgggagatc atgacgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagat
     5641 cggcccggaa aacggtacgg cacgcgaggg cctgcgcggg agatcatgac gtctccctcg
     5701 ggcgggccac gccggacgca ggtgggcgct cgggccgcgg cccgctctca ggggacgaga
     5761 acagtcgact ctggccgcaa tcgggtcaga gccgtgtcga acgtcgccaa ccgactgccc
     5821 ctggaagcgg cgagggctgc gaggtagacg tcggtgacct gccggtgacc gcgaacatcg
     5881 ctcaggtcga cgtgcccgta ggacagctca tcgccccaga actgacagct cggatgactg
     5941 ttcacctgct gcacgaggtg gctggccgtc gtcgcgctct ccccgatccg cagcaggaac
     6001 ctcacgagcg ccccttcgac cacggggcag accgcgaagc ccttgacctg cgtgagccac
     6061 cgggacgctc ggtcgtggtg ctcgtgatcg gccaccacca gggcgatgag gacgttcgcg
     6121 tcgagcaggt ggggcctcac tcatcgtcca ggaggtcggc gacctcggcc gacgtgaccc
     6181 gccgccccac ggccaggacg gggaggccgg tcctgtcgtc agtgcccaga acgaccggct
     6241 cctcgatctg ggccagaccc cgcaccgtca ggtccgcgag caccgcggac agggaccggc
     6301 cggtgcggac ggccagctcg gtggctcgac ggtgcacggc tggaggaaga tcgaccgtgg
     6361 tgcgcatgac tgcatcatac gtgatgcagg catgatgagg cctctccgtt gctgggcgga
     6421 ggtggacgag tcgggctgta ggccgggttc tgtcccgtgt cgccgttgcc agtgacgcgg
     6481 tgacggtcat ccatctcggg tggccgttgc cgaccacctc cagcgctcta cccgtgtact
     6541 cgggcgggcc gccctcgaac gcacactgtc tgagcttgct ccggatgggg tttaccgagc
     6601 cgacctggtc acccaggtcg ctggtggtct cttacaccac cgtttcaccc ttaccccgat
     6661 cgcttgcgcg gccggggcgg tctgttttct gtggcactgt cccgcgggtc gccccgggtg
     6721 gctgttggcc accatcctgc ccttcggagc ccggaccttc ctcggcggtg gttgcccacc
     6781 gacgcgaccg tccgcccgac tcgtccgccg ccagtgtagt gccggatcgt ggggcaggaa
     6841 ccggctcagc cctcgccggc gatctcgaac tccagcgccc cggccgcgac gtcggcacgc
     6901 acgagtcgaa ccgagacgtg ctcccccagc tcgactccgc tgcctcggct gcgggtcagc
     6961 accggcaggt cgacgagctg cacgacgaca cccttgtcgg tgcggtccac gattacggcg
     7021 tcgaacagct caccctcgcg tccgacgagc accgcggcct cggtggcgtc cgcgcaggcc
     7081 cgatcggccg agcgtgcgcg atggtccgag ctgcgcatgt gcgcggcgag gtccggcagg
     7141 gccgcgcgca cggcatccgg cacctcgtca ccacggcaca cggcctcgca gaccatgagc
     7201 ccgaaacggt cgacgagccg gcgcagcggt gcggtgacgt gggcgtaggg agccgcgacc
     7261 gcggcgtggt cacgttgcgc gggcagctcg ccgtcgaagg cggtgtaccc ggcgccccgg
     7321 aacagtccgc tggcctcgtg gatgagcgcc aggtggcggg ggtcggtgcg atccagcgag
     7381 cggatgaact cgccgtacgg catcgactgg ggccagtgcg ccccgagcgc ccgcgcgacc
     7441 cggcggtact gggccaccgc gccagcggcc ggcggcggca tcgtccgcag cacgcccaca
     7501 ccggcctcga gcatcatcga ggcggcggcc atcccggtaa ggagcgagat ctgcgcgttc
     7561 cactcctcgg ccggcacggg tggccggaac tcgatggtga agccgccgtc cgggcgctcg
     7621 acgatctgct gttccggcat cggcagactc gcaccgccgc gcacccgctc acgctcgatc
     7681 cggcgttcac cgatctcgcg cagcagcacg aagcgctcgt cgtcgctgcc cgcatcgatc
     7741 gcctcctgca cctcgtcgta cgccagtcgc tgcacgctgc gcaccatcgc ccgatacacc
     7801 tcggcggcgc ccagcacccc cgcggcgtcg aggtgcaggt cccagacgaa ggcgggccga
     7861 acggcatccg gcagcaggct cccggcccct tccgagagca cctcggggtg cagcggcacc
     7921 cgcaggtccg ggcagtagac gctctggccg cgctcacggg tctcggcgtc cagagcccca
     7981 ccgagccgca cgaaggcggg cacgtcggcg atggcgtagc gcacccggta cccgtcactg
     8041 tcccgctcga ggtgcatggc ctggtcgagg tccatcgacc cgggcgggtc cagcgtgatg
     8101 aacggcaggt cggtctcgtc gcgctcgggc agcgtcgggg gctgggccgc cacccgctcg
     8161 gcctcggcca aagcctcggc cgggtaggcg aggctgaggt cgaggtctgc gcgcaccgcc
     8221 gcgaaccggt cgcgcaggtc tgccagcaca ccgtcaggga tgtcggcgcc ggtgggggtc
     8281 aggcgaacgc gggggtgtgg catggcgcca acctaacctt tcgtaggctt ccctccgtgc
     8341 tgatcctgct cccgccgtcg gagtccaaga cgaaccggca ccgcggcaaa ccggttgacc
     8401 cggcgtcgtg gtccttccca gacctgaatc cggtccgggc gcgggtggcg caggcactgg
     8461 cggcggtgag cgcgtccccc gacgcgcccg ctctgctcga cgtctccccc gggttgctcg
     8521 acgacatcgc gcgcaacctc gtgctcgact ccgccccgag cactcccgcc gccgaggtct
     8581 acaccggcgt cctctacgac gccctcggcc tcgcctcgct cgacaccgca gcccgacgcc
     8641 gggccaaccg gtggatcgtc gtcgtgtcag cgctgtacgg ggcggtccgc cccaccgacc
     8701 gcatcgcggc ctaccgcctc gcgatgggca cgacactgcc cgacgtcggc gcactggccg
     8761 ccacctggcg cccggcgctg gagcaggtgc tgccacccct cgtcgggcgt ggggtcatcg
     8821 tcgactgccg ctctgcgccg tatgccgccg cgtggcggcc gggcggcgac ctcacccacc
     8881 gctgggtgca gatccgggtg cccggtgcca gccacatggc caaacacacc cgcggactgg
     8941 tcgcccgcca cctgtgcgag gtcggctgcg atgtgcggtc cgtgcccgcc ctggcagacg
     9001 tcgtcggcga ggccttcacg gtggccctgc acgaaccggc gagcgcgcga gccccttggg
     9061 tgctcgacgt gtcggcgccc tgatcaccga ggcgatcggg gcgctcgagc gcctcgtgcc
     9121 gatcgtcgtc ttcctgctcg cgatcacggt cgtggccgag atcgccgagc gcgccggggt
     9181 gttcgacgtc gccggccact gggtcgccca cgccggccgc caccgggcct gggccctgtg
     9241 ggtgctgttc gccctcgcga acaccgggtc cctgctgctg ccggtctcga acctcaccaa
     9301 cctgctcgcc ctgcactcgt tccagcggca gggcctcggc cacgtcgact acctcgccct
     9361 cgcgtgggcg ccggcgctgg cgtgcgtcgt cgtgaccctg ctgctgctcg tcgtgctgca
     9421 ccgtcgcacc ctggccgccc gctacgaggt cgacccgccg gcggatccgc acgacccggt
     9481 gctgctgcgc tggtcggccg tggtgtgcat cgccctcggc cccctgttcg cggtcggcgc
     9541 tcccccctgg gcggtgtcgc tcgtcgcggc cgccctcctg ctgtcggtcg ggctgtggcg
     9601 ggcgcccgac ctgctgcgcg ggctcccggt gccgtggctc atggccgcgg ggttcgtcgc
     9661 cgtggccgtg agcgtcgagg tcctgcaccg ccacgggctc gacgcggtgg tcgaccgggt
     9721 ggtcccctcg ggcaccgggg ccacggccct gctcgcggtc gcgggcctgg gcgccgggct
     9781 ggccaacgtc gtcaacaacc tgcccgccta cctcatcatc gagcccgcgg tggccgattc
     9841 ctccacccgc ctgatggcgc tgctcgtcgg ggtcaacgcg gggccgctcg tcacgccatg
     9901 ggcctccctc gccaccctgc tctggctggc tcggtgccgc agcgtcggcg tcgccatccc
     9961 gtggcgttgg ctggtccctg cgggggcggc gtgcgcagtg ctgtccgtgt cggcaggcac
    10021 cgtggcgctg gcgttcgtga cctgagcccc accggcgaca ggtgtgacgg gcgtcacggt
    10081 tagacaggcg cgcggatgcc gggtcgcacg acgtt
//
LOCUS       DAOSOC010000084         9929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_6787_length_9929_cov_9.312032, whole genome shotgun sequence.
ACCESSION   DAOSOC010000084 DAOSOC010000000
VERSION     DAOSOC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6787_length_9929_cov_9.312032"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PL014_07710"
     CDS             <1..460
                     /locus_tag="PL014_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT57012.1"
                     /translation="VGVLMVLALYCIVGFALTQTAWGRHIFAVGDDAESARLSGVASK
                     RILLSVYTVAGLIYGFAAWIQIGRAGSATANGIPDANLDSITAVVIGGTSLFGGRGGL
                     IGSLLGAIIVGSFTLGLSLAGVDQQWRLFAIGVLVIVAVSVDQWIRKVKA"
     gene            540..1370
                     /locus_tag="PL014_07715"
     CDS             540..1370
                     /locus_tag="PL014_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT57013.1"
                     /translation="MSTMERLPLAVDEIPPDRRAKYEADPILSARNLVITFGRVVGLD
                     GVNVDLYRGEVLAVIGDNGAGKSTLIKCLTGAYQPDSGDIQLNGEPVSFKRPQDAREA
                     GIETVYQQLAVIPALDIASNLYLAREERRPGPLGSVLRMLDKKGMKKRAGEAVEKLGI
                     QTIQNMGQAVETLSGGQRQAVAVARAAAFGSKVVVLDEPTAALGVKESGMVLDMVKQL
                     RDSGLAIILISHNMPHVWEAADRIHIQRLGGCAGVITPQSHDMGEGVAIMTGATTLSS
                     "
     gene            1512..1910
                     /locus_tag="PL014_07720"
     CDS             1512..1910
                     /locus_tag="PL014_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT57014.1"
                     /translation="MTIVVGYIRRPEGLAAVEAAIAEALLRGAKLVVVNTGHYGDYTH
                     PSFAAPADLEALDAQLTEHGIEHEVRQPTQGRAPADELLDIAEEVGATLVVIGLRRRN
                     PVGKLITGSTAQQVLLDSGCPVLAVKASNG"
     gene            complement(1987..2058)
                     /locus_tag="PL014_07725"
     tRNA            complement(1987..2058)
                     /locus_tag="PL014_07725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2024..2026),aa:Arg,seq:cct)
     gene            2111..3373
                     /locus_tag="PL014_07730"
     CDS             2111..3373
                     /locus_tag="PL014_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT57015.1"
                     /translation="MTTAPTHDPIQQRVVRTLAGTQVLGGVGLSAGVAVGALLAEDVS
                     GSAQFAGLGGTFQVLGAALIAIPMSRVMAAHGRRPGLLLGYLLAATGAIGLITAGVIR
                     NFPLLLVASVLFGGATASNSQARYAGADLAHPQHRARDLSIVVWATTIGSVVGPNLVG
                     PSEPVARALGLPLLTGPFLFSLLGLLLAIAVLLWRLRPDPLIEARQRRVADGDDSGAT
                     HGSVTRGLGVVAGIPNARLGILTLALGHVVMVSVMVMTPLHMNHGGADLEVIGFVISI
                     HILGMFAFSPLTGLAVDRFGGRAVAVLGSATLSAAALLASASPEGESWTLLLGLFLLG
                     LGWSCTLVAGSTLLTAAVPADERPGAQGASDLLMGLMAAGGGALAGVIVHQVSFEALA
                     LGALGVAISIGLAAALSTARPQIQDTRT"
     gene            3513..7448
                     /locus_tag="PL014_07735"
     CDS             3513..7448
                     /locus_tag="PL014_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOT57016.1"
                     /translation="MTDQPSTTDVVSPVEGDTTADPRRARVDTARAAWTRHLVDLGGR
                     NTLLWYRDLPTGTLDLTTAHPGGVAMLLAGKPTKLSDLVREPAALDEARRRARAIAAK
                     TRELAQERGILTGFLAIGMASWTVRLPDGRTAPRTPAAPVLLRACTLRATGAAQGDYT
                     LDLDGDLELNPVLVHYLASEQGIALDADAIEALATPGQTFDPYPIYAALAQACADVPD
                     FAVTPRLVLGTFSYAKLPMVADLAAQAETLADHDVVAALAGDPDALRSVRSDLDPHRL
                     DVDGPAPGRDVLVLDADSSQQEAIEAVRTGAHLVIHGPPGTGKSQTISNLIATLAADG
                     KRVLFVAEKRAAIDAVVGRLDRVGLGGLVLDLHAGAQGGRRLARDLVTALDQRSAPVR
                     AAGAPKTTSDAVASRVFDPTSTRRTTAAALTQHVAVLHDPREPWGVSLYEVQEAVCAF
                     ASTPVPPRSRVRLRGGELAALDRDELASAAATMAKLASIADWDGPGADDPWFGAGIRT
                     ADEADEARTRVQRLAGGGVDATASTLADVFRGIRLPETPTSRDWGRVLTTVGQVRDTL
                     EAFRPEVFDIPLDDLVTATGDKGYRRAVGSDLGWLERWRLRRQARALLRPGRPPASLH
                     AALAAASEQRIAWRELAGSGGRPEIPVELDRARLAHAALVDDIAWLDEHLPTSEPAAD
                     VRHLGSPDDHPAGHNGLTPLVDLDLAALQARADSLAARLDRLAVAPTVRGALDELTAS
                     GMGPLVDDLRTRAVPAEHVGVEVEWVWWSSLAEEIATRDPRVAAHDGRALTRTVAEFA
                     ESDRAVVAGNAERVVSAVGDRIAQVLRDHPAQETLLRAEGARARRLAPLRDLVTRCPD
                     LVLAVRPCWVMSPLVVASVLPPGQWFDVVVFDEASQIPPAEAVSAISRARQVVVAGDS
                     RQLPPTSFFTTVSESDPGAVGADEAITEGAESILDVLAAALPSRRLTWHYRSLDERLI
                     AFANAEVYEGALVTFPGTGIDPVVHHELVDGVGMVAEGEGAVETTIAEVDRVVELVLE
                     HARTRPQRSLGVIALGITHATRVEDAVRRAVGGLDDDALAFFDEDRPEAFFVKNLERV
                     QGDERDDVILTVGYGKTPHGRVLHRFGPLNTEGGERRLNVAITRARRSMTVVSSIAAG
                     DLDPARLKARGAVMLRDFLAYAATGSLPGATAPTSDAGASGSSADSPELSVGSGGSRS
                     VVLADLARQLRGHGLVVHEGVGSSADPVDLGVEDPHRPGRLVLAVESDGPQYAAMRTT
                     RDRDRLRAEQLERLGWRHLRVWSTDVFRDPARDVARILKAVGVRDASGE"
     gene            7540..7758
                     /locus_tag="PL014_07740"
     CDS             7540..7758
                     /locus_tag="PL014_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57017.1"
                     /translation="MPGQPEREKLAQPPVEGVPGTGRRKKRRRAVRSGTNPSAEDLPD
                     VVPPREEPAPGESAHERWLREQRPPHWE"
     gene            complement(7904..8272)
                     /locus_tag="PL014_07745"
     CDS             complement(7904..8272)
                     /locus_tag="PL014_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HOT57018.1"
                     /translation="MKGFKDFILRGNLIELAVAFIVGAAFATLVKAFTEVIIELLAKA
                     GGAPSFDEWKPFGMSTVGPFLTALVAFLIMAFIVYFFIVKPYELAKEKFVKSEEEDAA
                     PNADTVLLTEIRDALLRGRA"
     gene            complement(8419..9081)
                     /locus_tag="PL014_07750"
     CDS             complement(8419..9081)
                     /locus_tag="PL014_07750"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HOT57019.1"
                     /translation="MTPVPRPFPSVTGTGRRAQWRRWVARRVVAAGLAACAVVLATSA
                     ARPAPAPTVMVLVAAREVPAGAVLADGDVRPANVPGDAAQPGAITSLSDALGRRVAAG
                     LVTGEALTASRLVPRAAVDGLPRGRVALHVVAADPASVDLLPAGTIARVYPVAGGAPL
                     AVGARVLAVDPLVAPGGVLLAGESAPRGVVLSLTVAEADAVLRGHGALDGPVTVTVVG
                     SG"
     gene            complement(9120..9479)
                     /locus_tag="PL014_07755"
     CDS             complement(9120..9479)
                     /locus_tag="PL014_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT57020.1"
                     /translation="MPTYAYACTECDHRFEAVQSFSDDSLTVCPECEGRLRKVFNAVG
                     IVFKGGGFYRTDSRSGSSVTAGSSSSGSSSTSSGDSGSSSSSSSGGGSSESKTASSAG
                     SSSTGTATKTSSSTASA"
     gene            complement(9617..>9929)
                     /locus_tag="PL014_07760"
     CDS             complement(9617..>9929)
                     /locus_tag="PL014_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOT57021.1"
                     /translation="LGEGHHQVDLAVDATPLEELGADLLEIDVGPGSRIAGVRVFELR
                     LPVGANVSLVVRDGASFVPKDSDVLRHGDQLLVVTPQAVRDDTEKRLQSVSRGGRLAG
                     W"
BASE COUNT         1406 a         3754 c         3417 g         1352 t
ORIGIN      
        1 cgtcggtgtg ctcatggtgc tggcgctgta ctgcatcgtc gggttcgcgc tcacccagac
       61 cgcctggggt cgccacatct tcgcggtcgg tgacgacgcg gagtcggccc gcctgtcggg
      121 tgtcgcgagc aagcgcatcc tgctcagcgt ctacacggtg gccggcctca tctacggctt
      181 cgccgcgtgg atccagatcg gtcgcgccgg ctcggcgacg gccaacggca tccccgacgc
      241 caacctcgac agcatcaccg ccgtcgtcat cggtggcacc agcctgttcg gtggtcgcgg
      301 tgggctgatc ggcagcctcc tcggcgcgat catcgtcggg tcgttcaccc tggggctctc
      361 cctcgcgggg gtcgaccagc agtggcgcct cttcgcgatc ggcgtgctcg tcatcgtcgc
      421 cgtctcggtc gaccagtgga tccggaaggt gaaggcatga acatcacccc agaaagttct
      481 tcgctccttc ggcccatgca gagcatggga cccggctccg ataccttccg ggggccccga
      541 tgagcaccat ggaacggctg cccctggccg tcgacgagat cccgccggac cggcgggcga
      601 agtacgaggc cgacccgatc ctgtccgccc gcaacctcgt gatcaccttc ggtcgcgttg
      661 tcggtctgga cggggtcaac gtcgacctct accgaggtga ggttctcgcg gtcatcggtg
      721 acaacggtgc cgggaagtcc acgctcatca agtgcctgac aggcgcctac cagccggact
      781 ccggtgacat ccagctcaac ggtgagccgg tcagcttcaa gcgtccgcag gatgcccgag
      841 aagctggcat cgagaccgtc taccagcagc tggccgtcat cccggcgctc gacatcgcga
      901 gcaacctcta cctcgcccgc gaggagcgcc gtccgggtcc gctcggctcg gtgctgcgga
      961 tgctcgacaa gaaaggcatg aagaagcggg ccggtgaggc agtcgagaag ctcggcatcc
     1021 agacgatcca gaacatgggt caggctgtcg agaccctctc gggtggtcag cggcaggcgg
     1081 tcgccgtggc ccgcgccgcg gcattcggga gcaaggtcgt cgtcctcgac gagccgaccg
     1141 cagccctggg tgtcaaggag tcggggatgg tcctggacat ggtcaagcag ctgcgcgaca
     1201 gcggcctggc catcatcctg atcagccaca acatgccgca cgtgtgggag gccgccgacc
     1261 gcatccacat ccagcggctc ggaggctgcg ccggcgtcat caccccgcag tcccatgaca
     1321 tgggcgaagg cgtcgccatc atgaccggcg cgacgacgct gtcctcgtga ccggtcgatc
     1381 gcgaccgtcg ccctgacgac ggcgggacgc cgcacctgta ggacgaccag gtgcggcgtc
     1441 ccgtcctcac cccgactgaa gctcttcgca cgaccctcat gcaccactcc caacctgatc
     1501 cggagaccgt cgtgaccatc gtcgtgggct acatccgccg tcccgagggc ctggccgccg
     1561 tcgaggcggc catcgcagag gcgctactgc gcggcgccaa gctcgttgtc gtcaacaccg
     1621 ggcactacgg tgactacacc cacccgagct tcgcggcacc tgccgacctc gaggcactcg
     1681 acgcgcagct caccgagcac ggcatcgagc acgaggtccg gcagccaacg cagggcaggg
     1741 cacctgccga cgagcttctc gacatcgccg aggaggtggg cgccacactc gtcgtcatcg
     1801 gcttgcgccg ccgcaacccc gtcggcaagc tcatcaccgg ttcgaccgcc cagcaggtgc
     1861 tgctcgactc cgggtgcccc gtcctcgcgg tcaaggcatc caacgggtga ccgtcgaggg
     1921 tcgacaacgt cgagcatcac ccgagtcccg gatgccaaca atcaggcgaa ccaatccatc
     1981 cggctgtgcc cccggcagga ttcgaacctg cgacaccttc tttaggagag aagtgctctt
     2041 cccctgagct acgagggcgc acgcgaccac ggccgcgagc caccacaggg taccgggcat
     2101 acttccccga gtgaccacgg cgcccacgca cgaccccatc cagcagcggg tggtgcgtac
     2161 cctcgccggc acccaggtgc tcggcggagt cggcctgtcc gccggagtgg ccgtcggcgc
     2221 cctgctcgcc gaggacgtct cgggctccgc acagttcgcc ggcctcggcg gcacgttcca
     2281 ggtcctgggc gccgccctca tcgccatccc gatgtcccgc gtcatggccg cgcacggccg
     2341 ccgaccgggt ctgctgctcg gttacctgct ggccgcgacc ggcgcgatcg gcctcatcac
     2401 agccggggtg atccgcaact tcccgctgct cctcgtcgcg agcgtgctgt tcggcggcgc
     2461 cacggcatcc aacagccagg cgaggtatgc cggtgccgac ctcgcgcacc cccagcaccg
     2521 cgcgcgcgac ctcagcatcg tcgtgtgggc caccaccatc ggcagtgtcg tcggccccaa
     2581 cctcgtcggc ccgagcgagc cggtcgcgcg ggccctcggc ctgccgctgc tcaccggccc
     2641 gttcctgttc tcgctgctcg ggctcctgct cgcgatcgcg gtcctgctct ggcggctgcg
     2701 gcccgacccg ctcatcgagg cccggcagcg gcgggtggcc gacggcgacg actccggcgc
     2761 gacccacggc tcggtgacgc ggggcctggg ggtggtggcc ggcatcccca acgcgcgcct
     2821 cggcatcctc accctggccc tcgggcacgt cgtcatggtg agcgtcatgg tcatgacccc
     2881 gctgcacatg aaccacggcg gcgccgacct cgaggtcatc ggcttcgtca tcagcatcca
     2941 catcctcggg atgttcgcct tctccccgct cacgggcctg gccgtcgacc gcttcggcgg
     3001 acgagccgtc gccgtgctgg ggtcggcgac cctgtcggcg gccgcgctgc tggcgtcggc
     3061 ctcacccgag ggggagtcgt ggaccctgct cctcggcctg ttcctgctcg gcctgggctg
     3121 gtcgtgcacc ctcgtcgccg ggtcgaccct gctcaccgcc gccgtgcccg ccgacgaacg
     3181 ccccggcgcg cagggggcct ccgacctgct catggggctg atggccgctg gcggcggagc
     3241 ccttgccggg gtgatcgtgc accaggtgag cttcgaggcg ctggccctcg gggccctcgg
     3301 ggtggcgatc tccatcggcc tggccgcggc cctgtcgacc gcccggccgc agatccagga
     3361 caccagaacc tgacgcaggg gtgccaaggg tgcggtggag cgcgcgtctg gcccccccgg
     3421 gggtcccggc cgggcggcgc cttggccaac cccacaccaa cccgcccccg ggcctgagca
     3481 cccggcaggc tcgacgttgt tagcctgcac aagtgaccga ccagccctcg acgaccgacg
     3541 tggtgagccc cgtcgagggc gacaccaccg ccgacccccg gcgcgcccgc gtggacaccg
     3601 cccgcgccgc ctggacgcgc cacctcgtcg acctcggcgg gcgcaacacg ctgctctggt
     3661 accgcgacct gccgacgggc accctcgacc tcacgaccgc ccatcccggc ggcgtggcga
     3721 tgctgctcgc gggcaagccg accaagctct ccgacctcgt gcgcgagccc gccgccctcg
     3781 acgaggcccg ccgtcgcgcc cgggcgatcg ccgccaagac gcgcgaactc gcccaggaac
     3841 gcggcatcct caccgggttc ctcgccatcg gcatggcgtc ctggacggtc cggctgcccg
     3901 acggccgcac cgccccccgc acccctgctg ccccggtcct gctgcgagcc tgcaccctgc
     3961 gcgcgaccgg cgcagcccag ggcgactaca ccctcgacct cgacggcgac ctcgaactca
     4021 accccgtgct cgtgcactac ctggcctccg agcagggcat cgccctcgac gccgacgcca
     4081 tcgaggcgct cgccaccccc ggccagacct tcgaccccta ccccatctac gccgcgctcg
     4141 cgcaggcctg cgcagacgtg cccgacttcg cggtgacccc ccgcctggtc ctcggcacgt
     4201 tctcctacgc gaagctcccg atggtcgccg acctcgccgc ccaggccgag accctggccg
     4261 accacgacgt cgtcgcggcc ctcgccggcg accccgacgc cttgcgctcg gtgcgctccg
     4321 acctcgaccc gcaccgcctc gacgtcgacg gcccggcacc ggggcgcgac gtcctcgtcc
     4381 tcgacgccga cagcagccag caggaggcca tcgaggcggt gcgcaccggc gcccacctcg
     4441 tcatccacgg cccgcccggc accggcaagt cgcagacgat ctccaacctc atcgcgacct
     4501 tggccgctga cggcaagcgg gtgctgttcg tcgccgagaa gcgtgcggcc atcgacgccg
     4561 tcgtcggtcg cctcgaccgg gtcggcctcg gcgggctcgt cctcgacctg cacgccggtg
     4621 cccagggcgg tcgccgcctt gcccgcgacc tcgtgacggc gctggaccag cggtccgcgc
     4681 ccgtccgtgc ggcaggcgcc ccgaagacca cgtcggatgc cgtggcctcc cgggtcttcg
     4741 accccacctc cacgcggcgc acgaccgcgg cggccctgac ccagcacgtc gcggtgctcc
     4801 acgacccgcg tgagccgtgg ggggtgtcgc tgtacgaggt gcaggaagcg gtctgcgcgt
     4861 tcgcgagcac gccggtcccg ccgcgctctc gggttcgcct gcgcggggga gagctcgcag
     4921 ccctcgaccg cgacgagctg gcctcggccg ccgcgacgat ggccaaactg gcgtcgatcg
     4981 ccgactggga cggcccggga gccgacgacc cctggttcgg ggccggcatc cgcacggccg
     5041 acgaggccga cgaggctcgc acccgggtgc agcgactcgc gggcggcggg gtcgacgcca
     5101 cggcgagcac cctcgccgac gtcttccgcg gcatccgcct tcccgagacg ccgacgagcc
     5161 gcgactgggg cagggtgctc acgaccgtcg gtcaggtgcg cgacaccctc gaggccttcc
     5221 gccccgaggt cttcgacatc ccgctcgacg acctcgtgac cgcgaccggc gacaagggct
     5281 accggcgcgc cgtcggctcc gacctcggct ggctcgagcg gtggcggctg cggcggcagg
     5341 cccgtgccct cctgcgcccc ggccgccccc cggcgagcct gcacgcggcc ctggccgccg
     5401 cgagtgagca gcgcatcgcc tggcgagaac tggctggctc cggcggtcgg cccgagatcc
     5461 cggtcgagct cgaccgggcc cggctggccc acgccgccct cgtcgacgac atcgcctggc
     5521 tggacgagca cctgcccacc agtgagcccg cggcggacgt ccggcacctt ggcagccccg
     5581 acgaccaccc tgccggacac aacgggctga ccccgctcgt cgacctcgac ctcgccgcgc
     5641 tccaggcccg ggccgactcg ctggccgcac gtctggatcg cctcgccgtg gcacccacgg
     5701 tgaggggggc gctcgacgag ctcacggcat ccggcatggg cccgctcgtc gacgacctgc
     5761 gcacgcgtgc ggtgccggcc gagcacgtcg gtgtcgaggt cgagtgggtg tggtggtcct
     5821 cgctcgccga ggagatcgcg acccgtgacc cgcgggttgc ggcacacgac gggcgggcgc
     5881 tgacacgcac cgttgccgag ttcgccgaga gcgaccgcgc ggtcgtcgcg ggcaacgcag
     5941 agcgggtcgt ctcggccgtc ggggaccgca tcgcccaggt gctgcgcgac caccccgcgc
     6001 aggagacgct gctgcgcgcc gagggagccc gggcccgacg gctcgcgccc ctgcgcgacc
     6061 tcgtgacccg gtgccccgac ctcgtcctgg cggttcggcc ctgctgggtg atgagcccgc
     6121 tcgtcgtcgc gtcggtgctg ccgccggggc agtggttcga cgtcgtcgtc ttcgacgagg
     6181 cctcacagat cccaccggcc gaggcggtgt cggcgatctc gcgggctcgc caggtcgtcg
     6241 tcgccgggga ctcgcgccag ctgccgccga cctctttctt cacgacggtc agcgagagcg
     6301 accccggggc cgtcggtgct gacgaggcca tcaccgaggg ggctgagtcg atcctcgacg
     6361 tgctcgccgc cgcgctcccg agccggcgcc tgacctggca ctaccgttcc ctcgacgagc
     6421 ggctgatcgc gttcgccaac gccgaggtct acgagggggc gctcgtgacc ttcccgggca
     6481 ccggcatcga ccccgtggtc caccacgaac tggtcgatgg tgtcggcatg gtcgcggagg
     6541 gggagggcgc cgtcgagacg accatcgcgg aggtcgaccg tgtcgtcgag ctcgtcctcg
     6601 agcacgcccg gacccgcccc cagcgatcgc tcggtgtcat cgcgctcggc atcacccacg
     6661 cgacgcgggt cgaggatgcc gtgcgtcgtg cggtcggtgg gctcgacgac gacgcgctcg
     6721 cgttcttcga cgaggaccgg cccgaggcgt tcttcgtcaa gaacctcgag cgcgtacagg
     6781 gcgacgagcg tgacgacgtc atcctcacgg tcggctacgg caagaccccc cacggccggg
     6841 tgctgcaccg gttcggcccg ctcaacaccg agggcgggga gcgccgcctc aacgtcgcca
     6901 tcacccgggc gcgccggtcg atgacggtgg tgtcgtcgat cgccgccggg gacctcgacc
     6961 cggcccggct caaggcgcgc ggtgccgtca tgctgcgtga cttcctggcg tacgccgcca
     7021 cgggatcgct gcccggggcc acggcaccga cctcggatgc cggtgcgtcg ggttcgtcgg
     7081 ctgactcacc tgagctctcg gtgggttcgg gtggctcgcg gtcggtggtg ctggccgacc
     7141 tggcccgcca gctgcgcggg cacggactgg tcgtgcacga gggcgtcggc tcctcagccg
     7201 acccggtcga cctgggcgtc gaggacccgc accggcccgg ccgcctcgtg ctggccgtgg
     7261 agtcggacgg gccgcagtac gcggcgatgc gcaccacccg cgaccgcgat cgattgcgcg
     7321 cggagcagct cgaacggctc gggtggcggc acctgcgggt gtggtccacc gatgtcttcc
     7381 gtgaccccgc gcgcgatgtc gcccggatcc tcaaggccgt gggggtgcgc gatgcctcag
     7441 gggagtgacg acccatcagc cgtgagtgcg gaagtggctg ccccagcgac cacttccgca
     7501 ctcacggccg atggtcgcgc agcgggaggt gcctggcagg tgcccgggca gcccgagcgc
     7561 gagaagctgg cgcagccacc ggtcgagggc gtgcccggaa cggggaggcg caagaagcgt
     7621 cggcgggccg tgcgcagcgg caccaacccg tcggcggagg acctgccgga cgtcgtgccg
     7681 ccccgcgagg agcctgcacc gggcgagagt gcccacgagc gctggctgcg cgagcagcgc
     7741 ccgccccact gggagtgagg cgggcgcgac gggcagtgtc gtacctgagg tgacctattg
     7801 gtcgatctcg gtacgttggg aagggtggcg ctgtccctgg ggtgacctat tggtcgatgt
     7861 cgggacgcgg gcggtgctgc ccgcacgagt cgcgccacgt gggtcaggcg cggccgcgca
     7921 ggagggcgtc gcggatctcg gtgaggagca cggtgtccgc gttgggcgcg gcgtcctcct
     7981 cctcgctctt gacgaacttc tccttggcga gctcgtacgg cttcacgatg aagaagtaga
     8041 cgatgaaggc catgatgagg aaggccacga gcgcggtgag gaacgggccg accgtcgaca
     8101 tgccgaacgg cttccactcg tcgaaactgg gcgcgccgcc ggccttggcc agcagttcga
     8161 tgatgacctc ggtgaaggcc ttgacgaggg tggcgaaggc ggcgccgacg atgaacgcga
     8221 cggcgagctc gatgaggttg ccgcgcagga tgaagtcctt gaaacccttc atgggtttgc
     8281 tccttgggtg ggttgaacga tgaccagcga atgtgggggg gctgcacccc gcgccgacaa
     8341 gcgtagacgg ggtcaagcgc aggcgtggca tcgaacacca cggcccggca cgcgcgtgac
     8401 atggagagcc cccggctctc acccactccc gacgacggtg acggtgaccg ggccgtcgag
     8461 ggcgccgtgc ccgcgcagca ctgcatcggc ctcggcgacg gtcagcgaca gcacgacacc
     8521 gcgtggggct gactcacccg caaggagcac tccacccggt gccacgagtg ggtcgacggc
     8581 gagcacccgc gcacccacgg cgagcggggc gccaccagcc accgggtaga cccgggcgat
     8641 ggtgcccgcc ggcagcaggt cgaccgacgc agggtctgcc gccaccacgt gcagtgccac
     8701 ccgaccccgg ggaagaccgt cgacggccgc ccgcggaacc agccggctcg ccgtcagcgc
     8761 ctcgcccgtg accaggcccg ccgccacccg ccgcccgagc gcatccgaca gtgacgtgat
     8821 cgccccgggc tgggccgcgt caccaggcac attcgccggg cgcacgtcgc catcggccag
     8881 cacggcaccc gcagggacct cgcgggcagc cacgagcacc atcaccgtcg gggcaggtgc
     8941 aggccgagcc gcgctcgtcg cgagcacgac ggcgcaggcc gccaaaccgg cggccaccac
     9001 gcgccgcgcc acccaccgtc gccactgggc acgccgaccg gtgccggtga cggagggaaa
     9061 ggggcgaggg acgggggtca tgccccgacg ctaggcgcgc cgggacggca tccgagatct
     9121 caggccgagg ctgtggacga cgaggtcttc gtcgccgtgc ctgtggacga cgaccccgct
     9181 gaggacgcgg tcttgctctc cgacgagccg ccacccgagc tcgacgacga cgaactgccc
     9241 gagtcgcccg acgacgtgct cgacgacccg ctcgaggacg acccggccgt caccgacgac
     9301 cccgagcggg agtcggtgcg atagaatccg ccgcccttga acacgatgcc gaccgcgttg
     9361 aagaccttgc gcaggcggcc ctcgcactcg gggcacacgg tgagcgagtc gtcactgaac
     9421 gactgcaccg cctcgaaacg gtggtcgcac tcggtgcacg cgtaggcgta cgtgggcacg
     9481 gtggtcctcc tgaactccgg cgacggggtg gggtcgagcg gaaagaaaca cggcccggcc
     9541 ggtgcggcga gccaacaccc aaggataaag gacgtctggc cggcccccga accggccacc
     9601 agccgggcac gagcagctac cagccggcca ggcgcccgcc gcggctcact gactgcaggc
     9661 gcttctccgt gtcgtcccgc accgcctgcg gggtgacgac gagcaactgg tcgccgtgcc
     9721 gcagcacgtc actgtccttg ggcacgaacg acgcaccgtc gcggaccacc aacgacacgt
     9781 ttgctcccac gggcaggcgc agctcgaaca cccgcacccc ggcgatcctc gaaccgggac
     9841 cgacgtcgat ctcgagcagg tcggcaccga gctcctccag cggggtggcg tcgacggcca
     9901 ggtcgacctg gtggtgcccc tcacccaga
//
LOCUS       DAOSOC010000085         9809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_6904_length_9809_cov_11.296904, whole genome shotgun sequence.
ACCESSION   DAOSOC010000085 DAOSOC010000000
VERSION     DAOSOC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6904_length_9809_cov_11.296904"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..884
                     /gene="lepB"
                     /locus_tag="PL014_07765"
     CDS             111..884
                     /gene="lepB"
                     /locus_tag="PL014_07765"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT57022.1"
                     /translation="MSAEPSKSSGDPETPGIRETSEADGTPAPAPARRKRSLPMFTEV
                     IIALIAVALVQTFLIKPFGVPSQSMENTLQIGDRIVVNRLDDDVRAGDVIVFGHGETW
                     QAKELPPADNLLLKGIRAFGDLTGIGPSSTSYTVKRIIGMPGQKVACCTDVGAVTVDG
                     KPLTEPYVFEDLPFVPGIQDCTTSPRSVRCFPEITVPSENLLVLGDHRSQSADSVVGC
                     RGVTQGQECAKFVPKERVIGPVVGRFWPLSEFGSLPHGN"
     gene            complement(933..4505)
                     /gene="smc"
                     /locus_tag="PL014_07770"
     CDS             complement(933..4505)
                     /gene="smc"
                     /locus_tag="PL014_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOT57023.1"
                     /translation="MYLKTLTLKGFKSFASATTMRLEPGITCVVGPNGSGKSNVVDAL
                     AWVMGEQGAKSLRGGKMEDVIFAGTAGRPPLGRAEVSLTIDNTDGALPIDYTEVTITR
                     TMFRGGGSEYAINGTNCRLLDVQELLSDSGLGREMHVIVGQGQLDTVLRATPEERRGF
                     IEEAAGVLKHRRRKERALRKLETMEANLTRVQDLTGEIRRQLGPLGRQAEAARRAAVI
                     QADARDARLRLLADDLVQLTSLLEQEVADETALIERRSAVENALEDVRTRLSDVERRS
                     AEAAPELTRAQERFVRLQSLRDRLDSTGSVAAERVRLLSQDQEHEATSGRDPEELRAQ
                     AEQARAAEAALLAEVAAATSELAAAVTARQEVEAAYAAEQDRLSRLARAAADRREGLA
                     KLAGQVAARRSRIEAGEAEIGRLEATVAAARARAAEAEQEFAQLEGGVAVEEEGEEGL
                     DEAYESAAARLAEIEEQIEALVARERDAERDRSSATARIEALELSLRRKDGAATLLAA
                     ADAEHAVIGTVAALLQVSGGHEAAVAAALGWASEALAVPSGEHAARALASLRETSGGR
                     AALLVGTTASPSDGVQWPALDGSAVWARDVVTAPADVRPAVEQLLERVALVPTAAHAL
                     ALVARGEGITAVTADGDVFGPGWARGGSSAGESLLELQAAVDESRERLDAATAQSEQA
                     RFALVAAREAASAVATEVDAALEALHESDARMAAVAERLGSLGAAVRTASAEAERTER
                     AIAVARENLDRDRAELEGLSARLAEAQEAPELETQEPSTDERDRLELEASRARTAETE
                     LRLTLRTREERARALKGRADSLEGAARNELAARERLRERRERRRREATVAEAVRAAAV
                     YAVARIGEALARATTLRDTAEAERAERDRAAVALRTELGTVQDELRDLTDTAHRDEVA
                     RARQVARIEQLQARAVEELGIDPEVLVEEFGPHQLVPHVVAPGEDPDEAPEPAPYVRE
                     VQEKRLRKAERGLAALGRVNPLALEEFAALEERHTFLTTQLEDLKRSKRDLLDIVKEI
                     DERVEEVFTEAFADTKVQFERVFERLFPGGEGRLVLTDPDNMLTTGIEVEARPPGKKV
                     KRLSLLSGGERSLVAVALLVAIFKARPSPFYIMDEVEAALDDTNLGRLITLFEELRDS
                     SQLIVITHQKRTMEVADALYGVSMRGDGVTTVVSQRIRDVAATA"
     gene            complement(4578..4898)
                     /locus_tag="PL014_07775"
     CDS             complement(4578..4898)
                     /locus_tag="PL014_07775"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOT57024.1"
                     /translation="MSDTPPDLARLRSSIDNIDAALVHLLAERFKATQQVGELKAAIG
                     MPPADPAREERQIARLRGLADEAGLDPQFAEKFITFVIAEVIRHHEALAADPDAAQNS
                     VDRA"
     gene            complement(4982..5281)
                     /locus_tag="PL014_07780"
     CDS             complement(4982..5281)
                     /locus_tag="PL014_07780"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOT57025.1"
                     /translation="MDEDQPIRRVTVFVRGEVQGVGFRWWVRARALELGLTGHARNTG
                     DGRVEVVAEGPVPALGALLTLLSEPVSTAGRPGHVSTVSAPLRGEPRGEVGFRER"
     gene            5347..7332
                     /locus_tag="PL014_07785"
     CDS             5347..7332
                     /locus_tag="PL014_07785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOT57026.1"
                     /translation="MSTVTAHSPGGRDATTGVPAPARAAEGAPRPGRLPGLDGLRALA
                     IVAVLTFHLDPSWLPGGFLGVDVFFVVSGFLITTLLVRERAATGGLDLPGFWTRRARR
                     LLPALLVCVPASVLLARLSEGDLLVGIGRQVLGAFTFTSNWLELAAGSDYFAATTPTL
                     FMNLWSLAVEEQFYLLWPLAVLVLLRVTRGYEPRAAVALGLAVLSAVLMAVHVDPQSP
                     TRAYYGTDTHVMGLMLGAALAFAWAAPHRAWTRTPWWGAHRRQVAAAALVTLLALMAT
                     AGEASAWTFRGGILLASLATTALVLVVVERPGRLQAVLELPAARWIGARSYGIYLWHW
                     PVFLVIGADLPVAPGSPAYLATRVWCVVMTLVIADLSYRFIETPVRRHGFRGTAGRAL
                     ARLRAMGTRGARVLWGLALVLALVVTVVLVTAPERTATEELITANEAALALEDTTASS
                     ADERRPANPGTATADAATTSPSPTASAAVADSTTADWTMPSGKEIDVFGDSMAVGSIH
                     ALRYYLPGIRVDGKSNRQWSAAPKYVAAKGDSLRRAVVIVLGTNAGTDLPVARKTLDA
                     IGPDRMVVLTTVHGRFARAEEDNAALRELVKGRPNVRLADWDAALKGTSGQLQSDGIH
                     PSLKGSHLFAKTVRQALADLSEERTGKTVVLKELPGP"
     gene            complement(7337..8200)
                     /gene="mutM"
                     /locus_tag="PL014_07790"
     CDS             complement(7337..8200)
                     /gene="mutM"
                     /locus_tag="PL014_07790"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOT57027.1"
                     /translation="MPELPEVEVVRRGLADHVVGRRLAEVELRGHRVARRHVAGPVDL
                     ADRLTGRRIAAARRRGKYLWLDLEEALGLVVHLGMSGQLLVEAADAPDEKHLHARFRF
                     ADDGPELRFVDQRTFGGLALADLDDNGVPTSIAHIARDLLDPDFDRAAVIRAVKARDS
                     AIKRVLLDQRVASGIGNIYADEALWRAGVHGERVAHSLSKPLIGTVLDHASDVMREAL
                     DQGGTSFDALYVNVNGASGYFDRSLAAYGQEGRPCRRCGTLIRREHFMNRSSFSCPRC
                     QPRPRARRQPA"
     gene            complement(8208..8951)
                     /gene="rnc"
                     /locus_tag="PL014_07795"
     CDS             complement(8208..8951)
                     /gene="rnc"
                     /locus_tag="PL014_07795"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOT57028.1"
                     /translation="MGRAASAPGALTPPRPVTELLDHLVQVTSSPVDEALLQRALTHR
                     SYAYEAGGLPHNERLEFLGDSVLGLVVTTTLHDAHPDTSEGQLAKLRASVVNMRALAE
                     VGRTLGLGDYVFLGRGEETTGGRDKDSILADTVEAVIGAVYQSGGITAATELVHHLVD
                     PLLAQAVTLGAGLDWKTSLQEISSKHGLGSPEYSVVEEGPEHAKCFRAEVHVGGRVLG
                     QGEGRTKKVAEQQAAALAWGSIRDAHEGA"
     gene            complement(8962..9156)
                     /gene="rpmF"
                     /locus_tag="PL014_07800"
     CDS             complement(8962..9156)
                     /gene="rpmF"
                     /locus_tag="PL014_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT57029.1"
                     /translation="MAVPKRKMSRSNTRSRRANWKAAPVATTTCPQCKSLTQPHVACP
                     SCGTYKGRHYGVAERTEHQG"
     gene            complement(9158..9736)
                     /locus_tag="PL014_07805"
     CDS             complement(9158..9736)
                     /locus_tag="PL014_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HOT57030.1"
                     /translation="MTRLDPRSEMVLDTKLVGRRPGTMSELTREVGAPSDFGTVVMRV
                     EQGQPLTLDLRLESVVEGVLVTGSVRGTATGACVRCLDPVTHPVDGAFQELFVYAERH
                     AHHHEVGDEDAAEESRVEDGLIDLEPLLRDTVVPALPFQPVCRPDCPGLCSECGKPLA
                     EDPDHQHDLIDPRWAALSALTENQPTPTEKRT"
BASE COUNT         1256 a         3614 c         3510 g         1429 t
ORIGIN      
        1 acaaaccaca ccggcatccg tggccgaatc gtcaccaagc ggcaccaccg gcgcagtccc
       61 cccgactccc ccggccacac gacgtcgggc ccgacgacta gggtaaccgc gtgagcgccg
      121 aaccctcgaa gtcgtccggc gatcccgaga cccccggcat ccgcgagacc tccgaggccg
      181 acggcacccc cgctcctgcc ccggcgcggc gcaagcgcag cctgccgatg ttcaccgagg
      241 tgatcatcgc gctcatcgca gtcgctctgg tgcagacatt cctcatcaag cccttcggtg
      301 tgccctctca gtcgatggag aacaccctcc agatcggcga ccggatcgtg gtcaaccgcc
      361 tcgacgacga cgtgcgggcc ggcgacgtca tcgtcttcgg tcacggcgag acctggcagg
      421 ccaaggagct ccccccggcc gacaacctgc tcctgaaggg gatccgcgcc ttcggcgacc
      481 tcaccggcat cggtccgagc agcacgtcgt acacggtcaa gcggatcatc ggcatgcccg
      541 gacagaaggt ggcgtgctgc accgacgtcg gtgccgtgac ggtggacggc aaaccgctca
      601 ccgagcccta tgtcttcgag gacctcccct tcgtacccgg catccaggac tgcacgacct
      661 cgccccgctc ggtgcgctgc ttccccgaga tcaccgtccc gagcgagaac ctcctggtgc
      721 tcggtgacca ccgatcgcag tcagcggact ccgtcgtcgg atgccgtggg gtcacccagg
      781 ggcaggagtg tgccaagttc gtgcccaagg aacgcgtgat aggccccgtc gtcggacggt
      841 tctggccgct cagcgagttc ggctccctgc cccacgggaa ctgacggcga cgctgtcgcg
      901 cacagccagc gccgtcgcgc agccacccgg cgtcaggccg tggcggcgac gtccctgatc
      961 cgctgggaga cgaccgtcgt cacaccgtca ccacgcatcg acaccccgta gagggcgtcg
     1021 gccacctcca tcgtccgctt ctggtgggtg atgacgatga gctggctgga gtcgcgcagc
     1081 tcctcgaaca gcgtgatgag ccgccccagg ttggtgtcgt ccagcgccgc ctcgacctcg
     1141 tccatgatgt agaacgggct cggccgcgcc ttgaagatgg cgacgagcag ggccacggcg
     1201 accagtgagc gctcgccacc ggagagcagc gagagccgct tgaccttctt gcccggtggg
     1261 cgggcctcga cctcgatgcc cgtggtcagc atgttgtcgg ggtcggtgag gacaaggcgc
     1321 ccctccccac cggggaagag ccgctcgaag acccgctcga actgcacctt ggtgtcggcg
     1381 aaggcctcgg tgaagacctc ctccacccgc tcgtcgatct ccttgacgat gtcgagcagg
     1441 tcgcgcttgg agcgtttgag gtcctcgagc tgggtggtga ggaaggtgtg ccgctcctcc
     1501 agggccgcga actcctcgag ggcgagcggg ttgacccggc ccagggcggc gagcccgcgc
     1561 tcggccttgc gcaaccgctt ctcctgcacc tcgcgcacgt agggcgcggg ctcgggagcc
     1621 tcgtcggggt cctcgccggg ggcgacgacg tgcggaacga gctggtgggg gccgaactcc
     1681 tcgacgagca cctcggggtc gatgccgagc tcctcgacgg cgcgggcctg cagctgctcg
     1741 atgcgggcga cctgacgtgc gcgggccacc tcgtcacggt gggcggtgtc ggtgaggtcg
     1801 cgcagctcgt cctgcacggt gccgagctcg gtgcgcagtg ccaccgctgc ccggtcgcgt
     1861 tcggcgcgtt cggcctccgc ggtgtcgcgc agcgtggtcg cccgggccag agcctcaccg
     1921 atgcgcgcca cggcgtagac ggcggcggcc cgcacggcct cggcgaccgt ggcctcgcgg
     1981 cggcggcgtt cacgccgttc gcgcagtcgc tcgcgcgcgg cgagctcgtt gcgggcggcc
     2041 ccttcgaggg agtcggcccg acccttgagg gcacgggccc gctcctcgcg ggtgcgcagg
     2101 gtcagccgaa gctcggtctc cgcggtgcgg gcgcggctgg cttccagctc gagccggtcg
     2161 cgctcgtcgg tcgacggttc ctgcgtctcg agctcggggg cctcctgcgc ctcggcgagt
     2221 cgcgccgaga gtccctcgag ctcggcccgg tcgcggtcga ggttctcgcg cgccacggcg
     2281 atcgcccgct cggtgcgttc ggcctcggcg gatgccgtgc gcaccgcagc gcccaggctc
     2341 ccgagccgct cggcgacggc cgccatgcga gcgtccgact cgtgcagggc ctcgagcgcc
     2401 gcatcgacct cggtcgcgac cgcactcgcg gcctcccggg ccgcgacgag tgcaaaccgg
     2461 gcctgctccg actgggcggt cgcggcatcc agccgttccc ggctctcgtc gacggccgcc
     2521 tggagctcga gcaggctctc ccccgcgctg ctgccacccc gggcccaccc ggggccgaag
     2581 acgtcgccgt cggccgtgac ggccgtgatc ccctcaccgc gagcgaccag cgccagggcg
     2641 tgcgcggcgg tcggcacgag cgccacccgt tcgagcagct gctcgaccgc aggtcgcacg
     2701 tcagcggggg cggtcacgac gtcgcgcgcc cacaccgccg acccgtccag cgcaggccac
     2761 tgcacgccat ccgacgggga tgccgtggtg ccgacgagca gcgcggcgcg cccgccgctg
     2821 gtctcgcgca acgacgccag agcgcgggcg gcgtgctcgc cgctcgggac ggcgagggcc
     2881 tcggaggccc acccgagcgc agcggcgacg gcagcctcgt ggcccccgct gacctggagc
     2941 aacgccgcga cggtgccgat gacggcgtgc tcggcgtccg cggcggcgag cagggtggcc
     3001 gcaccgtcct tgcggcgcag gctgagctcc agggcctcga tgcgggcggt cgcggaggag
     3061 cggtcacgct cggcgtcgcg ctcgcgcgcc acgagggcct cgatctgctc ctcgatctcg
     3121 gcgagtcgcg cagcggcgct ctcgtaggcc tcgtcgagcc cctcctcgcc ctcctcctcg
     3181 acggcgacgc cgccctcgag ctgggcgaac tcctgctcgg cctcggcagc acgggcgcga
     3241 gctgccgcca ccgtcgcctc gagtcggccg atctcggcct caccggcctc gatccggctg
     3301 cgccgggccg ccacctgccc ggcgagcttc gccagcccct cacgacggtc ggcggccgcc
     3361 ctggccagcc gcgacaggcg gtcctgctcg gctgcgtagg ccgcctcgac ctcctggcgg
     3421 gccgtcacgg cagcagccag ctcgctcgtg gccgcggcga cctcggcgag cagcgcggcc
     3481 tcggcggcgc gggcctgctc ggcctgggcg cgcagctcct cggggtcgcg cccggaggtc
     3541 gcctcgtgct cctggtcctg cgagagcagg cgcacccgct cggcagccac cgacccggtg
     3601 gagtcgagcc ggtcgcgcag gctctgcagg cgcacgaagc gctcctgggc gcgggtgagc
     3661 tcgggggcgg cctcggcgga ccggcgctcg acgtcggaca ggcgggtgcg cacgtcctcg
     3721 agggcgttct cgacggcgct gcgtcgctcg atcagcgcgg tctcgtcggc gacctcctgc
     3781 tcgagcaggc tggtgagctg cacgaggtcg tcggcgagca gccgcaggcg ggcgtcacgg
     3841 gcgtcggcct ggatgacggc ggcgcgacgg gcggcctcgg cctggcgacc gagggggccg
     3901 agctggcggc ggatctcacc ggtgaggtcc tgcacccggg tgaggttggc ctccatcgtc
     3961 tcgagcttgc gcagcgcacg ctccttgcgg cggcggtgct tgagcacgcc ggccgcctcc
     4021 tcgatgaagc cgcgacgctc ctcgggcgtg gcccgcagca cggtgtcgag ctggccctgc
     4081 ccgacgatga cgtgcatctc acggccgagg cccgagtcgg agagcagttc ctgcacgtcg
     4141 aggagccgac agttcgtgcc gttgatggcg tactcgctgc cgcccccacg gaacatcgtg
     4201 cgggtgatcg tcacctcggt gtagtcgatc ggcagggcgc cgtcggtgtt gtcgatggtg
     4261 aggctgacct cggcccggcc cagcggcggg cgtccggcgg tgccggcgaa gatgacgtcc
     4321 tccatcttgc cgccgcgcag gctcttcgcg ccctgctcac ccatgaccca ggcgagggcg
     4381 tcgacgacgt tggacttgcc cgacccgttg ggcccgacga cgcaggtgat gcccggctcc
     4441 aggcgcatcg tcgtcgccga ggcgaaggac ttgaacccct tgagggtcag cgtcttcagg
     4501 tacacggtgt cgtcgcagct tccgtcgggt ggcgcaggct cggcaggagc cggatgccgc
     4561 ccgactctac cgccgcgtca ggcccggtcc acggagttct gggccgcgtc ggggtctgct
     4621 gcgagggcct cgtggtggcg gatgacctcg gcgatgacga aggtgatgaa cttctcggcg
     4681 aactgcgggt ccagcccggc ctcgtcggcc aggccgcgca ggcgggcgat ctggcgctcc
     4741 tcgcgggccg ggtctgccgg cggcatcccg atggcggcct tgagctcgcc gacctgctgg
     4801 gtggccttga agcgctcggc gagcaggtgc acgagcgctg cgtcgatgtt gtcaatgctc
     4861 gagcgcaggc gtgccaggtc gggaggggtg tcgctcacgc cgtcatcgta ggaagccacg
     4921 gcatccgcgg cgctcccccg tccacccccc ggatgccgtc gagcctggtt ggcagcgccc
     4981 gtcaccgctc acggaacccg acctcaccgc gcggctcacc gcgcagcggg gccgacacgg
     5041 tgctgacgtg acccgggcgc ccggcagtgg acaccggctc ggagagcagg gtcagcaggg
     5101 cacccagggc ggggaccgga ccctcggcga ccacctcgac ccggccgtcc ccggtgttgc
     5161 gggcgtgccc ggtcaggccc agctcgaggg ccctggcccg cacccaccag cggaaaccga
     5221 cgccctggac ctccccgcgc acgaacaccg tgacgcggcg aatgggctga tcctcgtcca
     5281 tggacaccca gcgtaggcct gcgcgcctcc gcaccactgt caccacctgc ccctaccgtt
     5341 accgacatga gcaccgtgac ggctcacagc cccggcggac gggacgccac gacgggcgtg
     5401 cccgcaccgg cgcgtgccgc cgagggtgca ccgcgccccg gccgactgcc tggcctggac
     5461 ggcctgcgcg ccctggccat cgtcgcggtc ctgacattcc acctcgaccc gtcctggctg
     5521 cccggcgggt tcctcggggt cgacgtgttc ttcgtcgtct cggggttcct catcaccacg
     5581 ctcctcgtgc gcgagcgcgc ggccaccggc gggctggacc tcccggggtt ctggacccgc
     5641 cgcgcccgcc gcctgctgcc cgcgcttctc gtctgcgtcc cggcatccgt gctcctcgcc
     5701 cgcctctccg agggcgacct gctcgtcggc atcgggcgcc aggtgctcgg ggcgttcacg
     5761 ttcacgtcca actggctcga actggccgct ggcagcgact acttcgcagc caccacgccc
     5821 acgctgttca tgaacctgtg gtccctcgcc gtcgaggagc agttctacct cctgtggccg
     5881 ctcgccgtgc tggtgctgct gcgggtgacg cgggggtacg agccccgggc tgccgtcgcg
     5941 ctgggcctgg ccgtgctgtc ggcggtgctg atggcggtgc acgtcgaccc gcagagcccg
     6001 acccgcgcgt actacggcac cgacacccac gtcatggggc tgatgctcgg cgcggcgctg
     6061 gcgttcgcgt gggcagcccc gcaccgcgcg tggacgcgca ccccgtggtg gggcgcccac
     6121 cggcgtcagg tggccgcggc ggcgctcgtc acgctgctcg cactgatggc gacggcaggt
     6181 gaggcctcgg cgtggacgtt ccgcggtggc atcctgctcg cctcgctcgc gacgaccgcc
     6241 ctcgtgctcg tcgtcgtcga gcggcccgga cgcctgcagg cggttcttga actgcccgca
     6301 gcccgctgga tcggtgcccg cagctacggc atctacctct ggcactggcc ggtgttcctc
     6361 gtcatcgggg ccgaccttcc cgtggcaccc ggcagcccgg cctacctcgc gacccgcgtc
     6421 tggtgcgtcg tcatgaccct cgtcatcgcc gacctgtcct accggttcat cgagacgccc
     6481 gtgcgccggc acggcttccg cggcaccgcc ggtcgtgccc tggcccggct gcgggcgatg
     6541 ggcacccggg gcgctcgtgt cctgtggggt ctcgcgctgg tgctcgccct ggtcgtcacc
     6601 gtggtgctcg tgaccgcgcc ggagcgcacc gccaccgagg agctcatcac ggccaacgag
     6661 gccgccctcg ctctcgagga cacgacggca tcctcggctg acgagcgtcg gcctgcgaac
     6721 ccgggcaccg ccacagcgga tgccgccacg acctcaccca gccccacggc atccgccgcc
     6781 gtggcggact cgacgaccgc cgactggacg atgccctccg gcaaggagat cgacgtcttc
     6841 ggcgactcca tggccgtcgg gtcgatccac gccctgcgct actacttgcc cggcatccgc
     6901 gtcgacggca agtccaaccg ccagtggagc gccgcgccca agtacgtcgc cgccaagggt
     6961 gactccctgc ggcgagcagt ggtgatcgtg ctcggcacga acgccggcac cgacctgccg
     7021 gtcgcgcgca agaccctcga cgccatcggg ccggaccgca tggtggtgct gacgaccgtc
     7081 cacggccggt tcgcccgcgc cgaagaggac aacgcggcgc tgcgcgaact cgtcaagggt
     7141 cgaccgaacg tgcgcctcgc cgactgggat gccgccctga agggcaccag cggacagctc
     7201 cagagcgacg gcatccaccc gagcctgaag ggctcgcacc tgttcgccaa gacggtgcgc
     7261 caggccttgg ccgacctgtc ggaggaacgc accggcaaga ccgtggtgct caaggaactc
     7321 cccggcccct gagggctcag gcaggttggc gccgggccct cggacggggc tggcagcgcg
     7381 ggcaggagaa cgacgaccgg ttcatgaagt gctcgcggcg gatgagggtc ccgcagcggc
     7441 ggcagggccg gccctcctgg ccataggcgg cgagggagcg gtcgaagtag cccgacgcgc
     7501 cgttgacgtt gacgtagagg gcgtcgaagc tcgtcccgcc ctggtcgagg gcctcgcgca
     7561 tcacgtcgct cgcgtggtcg agcaccgtgc cgatcagcgg tttgctgagg ctgtgagcca
     7621 cccgctcgcc gtggacgccg gccctccaca gggcctcgtc ggcgtagatg ttgccgatcc
     7681 ccgaggccac ccgctggtcg agcagcacgc gtttgatggc gctgtcgcgg gccttgaccg
     7741 ccctgatgac cgcagcccgg tcgaagtccg ggtcgagcag gtcgcgggcg atgtgggcga
     7801 tggaggtcgg caccccgttg tcgtcgaggt cggcaagcgc caggccgccg aaggtccgct
     7861 ggtcgacgaa gcgcagctcc ggcccgtcgt cggcgaaccg gaagcgggcg tgcaggtgct
     7921 tctcgtccgg ggcgtcagcc gcctcgacga gcagctgccc gctcatgccg aggtgcacga
     7981 cgagaccgag cgcctcctcg aggtcgagcc agaggtactt gccgcggcgg cgggctgccg
     8041 cgatgcggcg accggtgagc cggtcggcca ggtcgaccgg gccggcgaca tggcgccggg
     8101 ccacgcggtg gccgcgcagc tcgacctcgg ccagtcgacg cccgacgacg tggtcggcca
     8161 gacctcgccg gacgacctcg acctcgggga gctcgggcac cggtgggtca ggccccttcg
     8221 tgggcgtcgc ggatgctgcc ccacgccagc gccgcggcct gctgctcggc caccttcttc
     8281 gtgcggccct cgccctgccc gagcaccctg cccccgacgt gcacctcggc ccggaagcac
     8341 ttcgcgtgct ccggcccctc ctcgacgacg gagtactcgg gtgatccgag cccgtgcttg
     8401 gaggagatct cctgcaggct ggtcttccag tccaggccgg ccccgagggt cacggcctgc
     8461 gcgagcagcg ggtcgacgag gtggtgcacg agctcggtgg ccgcggtgat cccaccgctc
     8521 tggtagacgg cgccgatgac cgcctcgacg gtgtcagcga ggatcgagtc cttgtcgcgc
     8581 ccgccggtgg tctcctcccc ccgcccgagg aagacgtagt caccgaggcc gagcgtgcgg
     8641 cccacctcgg ccagcgcgcg catgttgacc acggatgcgc gcagcttggc cagctggccc
     8701 tcgctggtgt ccgggtgggc atcgtgcagg gtcgtcgtga cgacgaggcc gagcacggag
     8761 tcgccgagga actcgaggcg ttcgttgtgc ggcagcccgc ccgcctcgta ggcgtaggag
     8821 cggtgcgtca gggcacgctg aagaagcgcc tcgtcgaccg ggctgctggt cacctggacc
     8881 aggtggtcca gcagctcggt gacggggcgc ggcggtgtca gtgcaccggg cgcggacgcc
     8941 gccctcccca tggggaagcg atcagccctg gtgctcggtg cgctcggcga cgccgtagtg
     9001 gcggcccttg taggtgccac acgacgggca cgcaacgtgc ggctgggtga gcgacttgca
     9061 ctgggggcag gtcgtcgtgg cgacgggagc cgccttccag ttggcgcggc gcgagcgggt
     9121 gttggagcgc gacatcttcc gcttcggaac ggccacgtca ggtcctcttc tctgtcggag
     9181 tgggctggtt ctcggtcagg gccgagaggg ccgcccatcg ggggtcgatc aggtcgtgct
     9241 ggtggtccgg gtcctccgcg agcggcttgc cgcactcgga gcacagcccg gggcagtcgg
     9301 gccggcacac cggctggaac ggcagtgcag gaaccaccgt gtcccggagc agcggttcga
     9361 ggtcgatcag accgtcctcg actcgcgact cctcggctgc gtcctcgtcc cccacctcgt
     9421 ggtggtgggc gtggcgctcg gcgtagacga acagctcctg gaaggccccg tcgaccgggt
     9481 gggtcacggg gtccaggcac cgcacgcagg cgccggtcgc cgtgccacgg accgaaccgg
     9541 tgacgagcac tccctcgacg acggactcca ggcgcaggtc gagggtcagg ggctggccct
     9601 gctcgacgcg catgacgacc gtgccgaagt cgctcggcgc accgacttca cgcgtcagct
     9661 cactcatcgt cccgggacga cggccgacga gcttggtgtc gagcaccatc tcggaccggg
     9721 ggtcgagtcg agtcatgagc cttgggtgtt gtcggtgtgg ttcggccacg cctcacggac
     9781 gcagaccgag ggacaaggtt accggtcac
//
LOCUS       DAOSOC010000086         9661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7035_length_9661_cov_9.944410, whole genome shotgun sequence.
ACCESSION   DAOSOC010000086 DAOSOC010000000
VERSION     DAOSOC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7035_length_9661_cov_9.944410"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1070
                     /gene="fbaA"
                     /locus_tag="PL014_07810"
     CDS             39..1070
                     /gene="fbaA"
                     /locus_tag="PL014_07810"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT57031.1"
                     /translation="MPIATPEIYADMLDRAKAGSFAYPAINITSSQTVTAAIRGFAEA
                     GSDGIIQVSTGGAEYASGSTIKNMVAGSLALAAYAEEVAKHYPVNIALHTDHCPKDKL
                     DGFVRPLIAASTERVKAGGLPIFQSHMWDGSAVPLEENLQIAQELLALTAAANIILEV
                     EIGVVGGEEDGVANEINDQLYTTTEDALKTVEALGLGEKGRYMAALTFGNVHGVYKPG
                     NVKLRPEILKECQDAIIAAHGSAAGSKPLDLVFHGGSGSLPEEISAAVDYGVVKMNVD
                     TDTQYAFTRPVADWMLKNYTGVLKIDGEVGNKKQYDPRAWGKEAEAGMAARVVEACQN
                     LRSAGTHQG"
     gene            1216..1617
                     /locus_tag="PL014_07815"
     CDS             1216..1617
                     /locus_tag="PL014_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HOT57032.1"
                     /translation="MTDLLGIPPTHLPDDPASAALEMGVAPDQVAAANPTSSLAWAVL
                     AEDAFADGQVIESYAYARTGYHRGLDALRRSGWRGQGPVPWEHEPNRGFLRALAALSR
                     AAEAIGEEEERHRCAEFLRASSATGARELGL"
     gene            1622..2446
                     /locus_tag="PL014_07820"
     CDS             1622..2446
                     /locus_tag="PL014_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57033.1"
                     /translation="MTPDPAASDGGLLLVLEEQLRREHERIEARRADLATTRDLLSRV
                     TSRALGARARAVEVIEDAHAPSVVSALLADTTGLLRNYVLTVAEGPALDAATMQANVD
                     RIRRGSPQRALYPATVLDAADGGRWMAQWAEVGEEQRVLPTTETEFAVFGSTAVVALA
                     AWDDPSRGYAIIRDPLVIRLYSAYFDLAWASAVPVAALSGAAGVGGAGGAGGAGDAPL
                     VELLELGLKDEAIARHLGVSLRTVRRHIAALMEVNGVDTRFQLGAALARQQRGVSR"
     gene            2539..3840
                     /locus_tag="PL014_07825"
     CDS             2539..3840
                     /locus_tag="PL014_07825"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOT57034.1"
                     /translation="MPAIVLVGAQWGDEGKGKATDLMGGDVDYVVKFNGGNNAGHTVV
                     IEGPDGTREKYALHLLPSGILTPTVTPVIGNGVVVDLEVLFQELDGLEARGVDTSRLV
                     VSANAHVIAPYNRTLDKVTERFLGARRIGTTGRGIGPTYADKMNRIGIRVQDIFDEGI
                     LRQKVEAALAFKNQVLAKIYNTRSVEADRVVQELLGYRERLYPMVCDTSLVLEKALDA
                     GANVLLEAGQATLLDVDHGTYPFVTSSSATSGGACTGSGIPPTRVSRVIAILKAYTTR
                     VGEGPFPTELFDDDGETLRTVGAEYGTTTGRPRRCGWLDVVIGRYANRINGVTDFVIT
                     KLDVLTGFDKLPICVAYEVDGVRHDEMPVNQSDFHHAKPVYEVLDGWSEDISGCRTFE
                     ELPANAQAYVLRVEELIGARVSAIGVGPGRHEIIQRHALLD"
     gene            complement(3849..>6014)
                     /locus_tag="PL014_07830"
     CDS             complement(3849..>6014)
                     /locus_tag="PL014_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT57035.1"
                     /translation="AELALEQAHVDRVYAELEKASRRAADVEADGLARGRTSRTGDVR
                     DEEMTGLFERDALVYAAARRRSMIDKQYEGLVFGRLDLGTEQSTAAEREVRYIGRLGV
                     RDDDYEPLVVDWRAPAASAFYRATPVDPMGVVRRRVLRCSGATVVGAEDDLMVPAAPD
                     DLVVLGDGALMAALTRTRGRQMRDIVATIQRHQDEAIRAPSRGVTEITGGPGTGKTVV
                     ALHRAAYLLYSERRRFESGGILVVGPSAAYTAYIERVLPSLGEESVALRALGDVVDGM
                     TATRLDSPEVAAIKGSLRIRRLLARLASRAPAGSPTTFRAFIAGHAVRLDEPVLRRVR
                     SDVLRGHQHNLAADAARTALAEAAWRSVRVGERDAFLEAFEGSRDVDDFVAAWWRPLD
                     PRELLLGLADTEYAYEVSRGVLEQEEAAALAASYRDALAHGSWSVADAALVDDLVARL
                     AAVQVVEREDVGFYDIEELDNLTAYGVQDVRAGIDRRVTTNSQVGIVTPADARERLMM
                     GRIERSSWYAHVLVDEAQDLSPMQWRMLARRGRSASWTVVGDAAQASWGDLAEAGRAR
                     DEAFSGQARQAFHMDTNYRNAREIFDYARDVILPLVPDADIPDAVRETDVDPVDRLVD
                     GSMAQATSDAVEQLLAEVDGSIAVIAAGRWMEQVAALDGSGGGRVQVIDPLSTKGLEW
                     DATVVVDPDGITAESPGGVRVLYVVLTRAAHRMHVLRPT"
     assembly_gap    6015..6114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6305..7576
                     /gene="purD"
                     /locus_tag="PL014_07835"
     CDS             6305..7576
                     /gene="purD"
                     /locus_tag="PL014_07835"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOT57036.1"
                     /translation="MKVLVVGSGAREHAIVRSLCLDPSVDEVVAAPGNPGIADLARCV
                     ALPGGVTDADAVVSLAVETGVDLVVIGPEAPLVAGVADAVRAAGIACFGPSARAAMLE
                     GSKAFAKAVMSSAEVPTGLAHVCTTMEEVADALDALGAPHVVKDDGLAAGKGVVVTED
                     RAQALDHARECLAKPDGAVVIEEYLSGPEVSLFCLCDGVSVVPLTPAQDFKRVGDGDA
                     GPNTGGMGAYSPLGWAPDGLVDDVVRRVAQPVIDEMARRGTPFVGVLFIGLALTPRGP
                     RVIEFNARFGDPETQVVLTRLLTPLGGLLLAAATGRLEEVPPLRWSAEHAVTVVVAAQ
                     SYPATPRTGDPITGLPAGDGAYVLHAGTALDASGQLVSAGGRVLSVVATGDTLASARE
                     RAYAAVAGIDLPGSHHRSDIALLAERGEVTI"
     gene            complement(7737..8672)
                     /locus_tag="PL014_07840"
     CDS             complement(7737..8672)
                     /locus_tag="PL014_07840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HOT57037.1"
                     /translation="MDLRIFTEPQQGATYDDLLAVAKAAEELGFDAFFRSDHYLHMSG
                     DGLPGPTDAWTTLAGLARDTDRIRLGTLVTSATFRLPGPLAVMVADVDRMSGGRVELG
                     LGAGWFEAEHAAYGIPFPPLGERFDRLEEQLAVITGLWSTPPGSTFEHDGPHYPVRAN
                     PGLVAPEQRGGVPIIVGGGGRRRTPALAARFAAEFNAPFAPVAQTGRLFERVDAACRD
                     VGRDPATLTHSAAQVVCVGRDDATVRRRAQAIGRDVDDLVENGVAGSFEQARDRLGRL
                     AEVGTTRVYLQVLDLADLDHLADVHAALAAPPVPA"
     gene            8858..>9661
                     /locus_tag="PL014_07845"
     CDS             8858..>9661
                     /locus_tag="PL014_07845"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT57038.1"
                     /translation="MPDAPAPLTLPGYAHVYSGKVRDLYAPVDPASGEPRPDQLLLVA
                     SDRISAFDFILDSPIPDKGEVLTRLSLWWFAQLEDLVPNHVISTDVPDAVRGRAVLVE
                     RLEMLPVECVARAYLTGGGLGEYAATGAVSGIALPAGLVDGDRLPEPIFTPSTKAPMG
                     EHDEPIPFAAVEAEIGSDLAARVRDLTVAILKRGNEIAAARGILLADTKVEFGLRGDE
                     VVLADEVLTPDSSRFWPADQWEPGHPQPSFDKQFVREWLTSPASGWSKSS"
BASE COUNT         1310 a         3394 c         3545 g         1312 t
ORIGIN      
        1 tcgtaccggc cccgtcacaa ccccctgagg agatcgccgt gcccatcgca acgcccgaga
       61 tctacgccga catgctcgac cgcgccaagg ctggctcgtt cgcgtacccg gccatcaaca
      121 tcacgtcgag ccagacggtg accgcggcga tccgtggctt cgccgaggcc ggctccgacg
      181 gcatcatcca ggtctccacg ggcggcgccg agtacgcctc cgggtcgacg atcaagaaca
      241 tggtggccgg ttcgctcgcc ctcgcggcgt acgccgagga ggtcgccaag cactacccgg
      301 tgaacatcgc cctgcacacc gaccactgcc ccaaggacaa gctcgacggc ttcgtgcgcc
      361 cgctcatcgc ggcctccacc gagcgcgtca aggccggcgg tctgccgatc ttccagagcc
      421 acatgtggga cggctcggcc gtgccgctcg aggagaacct ccagatcgcc caggagctgc
      481 tcgccctcac ggccgctgcc aacatcatcc tcgaggtcga gatcggcgtc gtcggtggcg
      541 aggaggacgg tgtcgccaac gagatcaacg accagctcta cacgaccacc gaggacgccc
      601 tgaagaccgt cgaggccctc gggctcggtg agaagggtcg ctacatggcg gcgctgacct
      661 tcggcaacgt gcacggcgtc tacaagccgg gcaacgtcaa gctgcgcccc gagatcctca
      721 aggagtgcca ggacgccatc atcgccgccc acggcagcgc ggccggctcc aagccgctcg
      781 acctcgtctt ccacggcggg tccggctcgc tgcccgagga gatctcggcc gcggtcgact
      841 acggcgtcgt gaagatgaac gtcgacaccg acacccagta cgccttcacc cgcccggtcg
      901 ccgactggat gctgaagaac tacaccggcg tcctgaagat cgacggcgag gtcggcaaca
      961 agaagcagta cgacccccgc gcctggggca aggaggccga ggccggcatg gctgctcgcg
     1021 tcgtcgaggc ctgccagaac ctgcggtcgg cgggtaccca ccagggctga cccctccggc
     1081 actgattgcg ccccacccac ctcgcgccag ccgcgccggg tggctggggc gcaatcagtg
     1141 ggtatgacgg atgccgtgcg cccaccgtcg tggtgggcgc acggcatccg ctttcccccc
     1201 tttacgctgg ggcccatgac cgacctgctg ggaatcccgc cgacgcacct gcctgacgac
     1261 ccggcatccg ccgccctgga gatgggggtg gcgcccgacc aggtggctgc ggccaacccg
     1321 acgtcgtccc tggcgtgggc ggtgctcgcc gaggacgcgt tcgccgacgg gcaggtcatc
     1381 gagtcgtacg cctacgcccg caccggctac caccgcggcc tcgacgcgct gcgccgctcg
     1441 ggctggcgcg gccagggccc ggtgccgtgg gagcacgagc cgaaccgcgg gttcctgcgc
     1501 gccctcgcgg ccctgtcgcg ggcggccgag gccatcggcg aggaggagga gcgccaccgg
     1561 tgcgccgagt tcctgcgcgc ctcgagtgcc accggcgccc gagagctggg gctctgacgc
     1621 cttgaccccc gacccggcgg cgagcgacgg ggggctgctc ctcgtcctgg aggagcagct
     1681 gcggcgcgag cacgagcgga tcgaggcgcg ccgggccgac ctcgcgacga cccgcgacct
     1741 gctctcgcgg gtcacgagcc gggcgctcgg tgcccgcgca cgagccgtcg aggtcatcga
     1801 ggacgcccac gcgccctcgg tcgtcagcgc cctgctggcc gacacaacgg gcctgctgcg
     1861 caactacgtg ctgaccgtcg cggaggggcc ggcgctggac gcggcgacga tgcaggccaa
     1921 cgtcgaccgc atccggcggg ggtcgccgca gcgggcgctg tacccggcga cggtgctgga
     1981 tgccgcggac ggggggcgct ggatggccca gtgggccgag gtcggggagg agcagcgggt
     2041 gctgcccacg accgagaccg agttcgccgt gttcggctcg accgcggtcg tggcgctcgc
     2101 agcctgggac gacccgtcgc gggggtacgc gatcatccgt gacccgctcg tcatccggct
     2161 ctacagcgcc tacttcgacc tcgcgtgggc ctcggcggtg ccggtcgccg cgctgtcggg
     2221 agctgcgggt gtggggggcg ccgggggtgc cggtggcgcc ggggacgcgc cgctcgtcga
     2281 gctgctcgag ctcggcctga aggacgaggc gatcgcgcgc cacctcgggg tgagcctgcg
     2341 caccgttcgc cggcacatcg ccgcgctcat ggaggtcaac ggggtggaca cccgcttcca
     2401 gctcggtgcg gccctggctc ggcagcagcg gggagtctcg cggtagggtg cggtgaacga
     2461 ggccccggag cgtaggcgcg aggggccttc cgcgcgtcga gggcatctgg ctccggcgca
     2521 cgtccggaag gagtcggcat gccggcgatc gtgctggtcg gagcccagtg gggcgatgag
     2581 ggcaagggca aggcgaccga cctgatgggg ggtgacgtcg actacgtcgt gaagttcaac
     2641 ggtggcaaca acgccggcca caccgtcgtc atcgaggggc cggacggcac gcgggagaag
     2701 tacgcgctcc acctgctgcc cagcggcatc ctcactccga cggtgacgcc cgtcatcggc
     2761 aacggcgtcg tcgtcgacct cgaggtgctc ttccaggagc tcgacgggct cgaggctcgc
     2821 ggcgtcgaca cctcacgcct ggtcgtctcg gccaacgcgc acgtcatcgc gccgtacaac
     2881 cgcacgctcg acaaggtgac cgaacgcttc ctcggcgccc gccggatcgg cacgaccggc
     2941 cgggggatcg gcccgaccta cgccgacaag atgaaccgca tcggcatccg cgtccaggac
     3001 atcttcgacg aggggatcct gcgccagaag gtcgaggcgg cgctggcgtt caagaaccag
     3061 gtgctcgcga agatctacaa cacccgctcg gtcgaggccg accgcgtcgt ccaggagctg
     3121 ctcggctacc gcgagcggct gtaccccatg gtctgcgaca cctccctggt gctggagaag
     3181 gcgctggatg ccggtgcgaa cgtgttgctg gaggccggcc aggcgaccct gctcgacgtc
     3241 gaccacggca cgtacccgtt cgtcacgtcg tcgtcagcca cctcgggtgg ggcgtgcacc
     3301 gggtccggca tcccgccgac ccgggtgtcg cgggtcatcg cgatcctcaa ggcgtacacg
     3361 acccgcgtcg gtgaggggcc gttcccgacc gagctcttcg acgacgacgg cgagacgctg
     3421 cgcacggtcg gcgccgagta cggcacgacc accggccgcc cgcgacgctg cggctggctc
     3481 gacgtcgtca tcggccgcta tgccaaccgg atcaacggcg tcaccgactt cgtcatcacc
     3541 aagctcgacg tgctcaccgg gttcgacaag ctgccgatct gtgtcgccta cgaggtcgac
     3601 ggcgtccggc acgacgagat gccggtcaac cagagcgact tccaccacgc caagccggtc
     3661 tacgaggtgc tcgacggctg gtcggaggac atctcggggt gccgcacctt cgaggagctt
     3721 cccgcgaacg cgcaggccta cgtgctgcgg gtcgaggagc tcatcggggc tcgcgtctcg
     3781 gcgatcggcg tcggcccggg ccggcacgag atcatccagc gccacgccct cctggactga
     3841 gccccagttc aggtggggcg caggacgtgc atccggtgcg cggcgcgggt cagcacgaca
     3901 tagagcaccc gcacccctcc gggtgactcg gcggtgatgc cgtcgggatc gaccacgacg
     3961 gtggcgtccc actccaggcc cttggtcgac agcgggtcga tgacctgcac ccggccgccg
     4021 ccgctgccgt cgagcgcggc gacctgctcc atccagcgcc ccgccgcgat gaccgcgatc
     4081 gagccgtcga cctccgcgag aagctgctcc acggcatccg acgtcgcctg cgccatcgag
     4141 ccgtcgacca gccggtcgac ggggtcgacg tcggtctcgc gcacggcatc cgggatgtcg
     4201 gcgtcgggca cgagggggag gatgacgtcg cgggcgtagt cgaagatctc gcgggcgttg
     4261 cggtagttgg tgtccatgtg gaaggcctgg cgcgcctgcc cggagaaggc ctcgtcgcgg
     4321 gcccggccgg cctcggcgag gtcgccccac gaggcctggg cagcatcgcc gaccacggtc
     4381 cacgatgccg agcgaccacg acgcgcgagc atccgccact gcatggggga gaggtcctgc
     4441 gcctcgtcga cgagcacgtg cgcgtaccag gacgaccgct cgatgcggcc catcatgagg
     4501 cgctcccggg cgtcggccgg cgtgacgatg ccgacctgcg agttcgtcgt cacccggcgg
     4561 tcgatcccgg cacgcacgtc ctgcacgccg taggccgtga ggttgtcgag ctcctcgatg
     4621 tcgtagaagc cgacgtcctc gcgctcgacg acctgcaccg cggccaggcg ggccacgagg
     4681 tcgtcgacga gggccgcgtc ggccaccgac cacgagccgt gcgccagggc gtcacggtac
     4741 gacgcggcga gggcggccgc ctcctcctgc tcgagcaccc cacggctgac ctcgtaggcg
     4801 tactcggtgt cggcgaggcc gagcagcagc tcgcgcgggt cgagcgggcg ccaccacgcg
     4861 gcgacgaagt cgtcgacgtc gcgcgagccc tcgaacgcct cgaggaaggc atcacgctca
     4921 ccgacccgca ccgagcgcca ggcggcctcc gcgagagccg tgcgcgcggc atccgccgcg
     4981 aggttgtgct ggtggccgcg aagcacgtcg gagcggaccc ggcgcaggac gggctcgtcg
     5041 aggcggacgg catggccggc gatgaaggcc cggaaggtcg tgggcgaccc ggcgggcgct
     5101 cgggaggcga ggcgtgcgag caggcggcgg atgcgcagcg agcccttgat ggcggcgacc
     5161 tcggggctgt cgaggcgggt ggcggtcatg ccgtcgacga cgtcgccgag ggcccgcagc
     5221 gccaccgact cctcacccag gctcggcagg acgcgctcga tgtaggcggt gtaggcggcg
     5281 gacgggccga cgacgaggat gccgccgctc tcgaaccggc gccgctcgga gtacagcagg
     5341 tacgcggcac ggtgcagcgc cacgacggtc ttgccggtgc cggggccgcc ggtgatctcg
     5401 gtgacgccgc gcgaaggggc ccggatggcc tcgtcctggt gacgctggat ggtcgcgacg
     5461 atgtcgcgca tctggcggcc gcgggtgcgc gtcagggccg ccatgagggc accgtcaccg
     5521 aggacgacga ggtcgtcggg ggcggcgggc accatgaggt cgtcctcggc gccgaccacc
     5581 gtggcgccgg agcagcgcag cacgcgccgg cgcacgacgc ccatcgggtc gacgggggtg
     5641 gcgcggtaga aggcggatgc cgcgggggcg cgccagtcga cgacgagcgg ttcgtagtcg
     5701 tcgtcgcgaa ccccgaggcg gccgatgtag cgcacctcgc gctcggcggc ggtggactgc
     5761 tcggtgccga ggtcgagccg gccgaagacc aggccctcgt actgcttgtc gatcatcgag
     5821 cgccggcggg ccgcggcgta caccagcgcg tcgcgctcga acaggccggt catctcctcg
     5881 tcgcgcacgt caccggtgcg cgaggtgcga ccgcgggcca ggccgtcggc ctcgacgtcg
     5941 gccgcgcgtc ggctggcctt ctcgagctcg gcgtagaccc ggtcgacgtg ggcctgctcg
     6001 agggcgagct cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccgcg
     6121 tcggggacgt cgggcgtggt ctcggacgtg ttgtcgggcg ccgtgggtcg ggcggtcacg
     6181 aggtgagctc tcctggctgg gctgctggtg ctgctggtgc acgtggcgcg agccacggaa
     6241 ctcaccagtc tagccgcgcg gaccgggcag ggcctgcgag cggtcctacc tctagattgg
     6301 gcgcgtgaag gttctcgtcg tcgggtccgg ggcccgcgaa cacgccatcg tccgctccct
     6361 ctgcctcgac ccgtcggtcg acgaggtcgt cgccgccccg gggaaccccg gcatcgccga
     6421 cctcgcgcgc tgtgtcgccc tgccgggtgg ggtcaccgac gccgacgccg tggtgtccct
     6481 ggctgtcgag accggggtcg atctcgtcgt catcgggccg gaggcgcccc tggtcgcggg
     6541 cgtggcggat gccgttcgcg ccgccggcat cgcgtgcttc gggccgtcgg cgcgggcggc
     6601 catgctcgag gggagcaagg ccttcgccaa ggcggtcatg tcgtcggcgg aggttccgac
     6661 cggtctggcg cacgtgtgca cgaccatgga ggaggtcgct gacgccctcg acgccctcgg
     6721 ggccccgcac gtcgtcaagg acgacgggct ggccgccggc aagggggtcg tcgtcaccga
     6781 ggaccgggcg caggcgctgg accacgcccg tgagtgcctg gccaagcccg acggtgcggt
     6841 ggtcatcgag gagtacctct ccgggcccga ggtctcgctg ttctgcctgt gcgacggggt
     6901 gagcgtggtt ccgctcaccc ccgcgcagga cttcaagcgc gtcggcgacg gggatgccgg
     6961 gcccaacacc ggcgggatgg gcgcctacag cccactcggg tgggccccgg acgggctcgt
     7021 cgacgacgtc gtgcgccgcg tggcccagcc ggtcatcgac gagatggcgc gccgaggcac
     7081 gcccttcgtc ggggtgctct tcatcgggtt ggccctgacc ccgcgcgggc cgcgggtcat
     7141 cgagttcaac gcgcggttcg gcgaccccga gacccaggtc gtgctgacca ggctgctgac
     7201 cccgctcggt ggcctgctgc tggctgcggc gacgggccgg ctcgaggagg tgccgccgct
     7261 gcggtggtcg gccgagcacg ccgtcaccgt ggtcgtggct gcgcagagct acccggcgac
     7321 gccgcgcacc ggtgacccga tcaccgggtt gcccgcgggt gacggggcgt acgtcctgca
     7381 cgccggcacg gcgctggacg cctcggggca gctggtctcc gccggtgggc gggtgctctc
     7441 ggtcgtcgcc accggtgaca ccctggcctc ggcgcgggag cgggcctacg cggccgtcgc
     7501 cggcatcgac ctgccgggct cgcaccaccg ctcggacatc gccctgctcg ccgagcgcgg
     7561 cgaggtcacc atctgattcg ggccgtacgt ggagtgacca ataggtcgct gtgggtacgc
     7621 acggtgtggg tcgggccgct cgcccgccgc gagggcccgc gcaacggcgc gaacggcatc
     7681 cggaaggggt ttgagctggt cctcgatgtt ggcggtcacc ggcaggtcga cggcggctac
     7741 gcggggaccg ggggtgccgc cagcgcggcg tgcacgtcgg cgaggtggtc gaggtcggcg
     7801 aggtcgagca cctgcaggta cacccgcgtc gtgccgacct cggcgagccg gccgaggcgg
     7861 tcacgtgcct gctcgaagct gccggcgacg ccgttctcca cgaggtcgtc gacgtcgcgc
     7921 ccgatggcct gcgcccggcg gcgaaccgtg gcgtcgtcgc gaccgacgca cacgacctgg
     7981 gcggcggagt gggtgagcgt ggcagggtcg cgccccacgt cgcggcatgc cgcgtcgacg
     8041 cgctcgaaga gccgaccggt ctgcgccacc ggggcgaacg gcgcgttgaa ctcggcggcg
     8101 aagcgtgcgg cgagcgcagg cgtgcggcgt cggccgccac cgccgacgat gatcggcacc
     8161 cctccgcgct gctcgggtgc cacgaggccg gggttggcgc gcaccgggta gtgcggcccg
     8221 tcgtgctcga aggtggaccc gggtggtgtc gaccacaggc cggtgatgac ggcgagctgc
     8281 tcctcgagcc ggtcgaagcg ctcacccagg ggtgggaagg ggatgccgta cgcggcgtgc
     8341 tcggcctcga accacccggc gccgaggccc agctcgaccc ggccgccgct catccggtcg
     8401 acgtcggcga ccatcaccgc cagcggaccc ggcagccgga acgtcgcgga cgtgacgagc
     8461 gtgccgagcc ggatgcggtc ggtgtcgcgg gccaggccgg ccagggtggt ccatgcgtcg
     8521 gtggggcccg gcaggccgtc tcccgacatg tgcaggtagt ggtcggagcg gaagaacgcg
     8581 tcgaagccga gctcctcggc ggccttggcc acggcgagga ggtcgtcgta cgtcgcgccc
     8641 tgctggggct cggtgaagat gcgaaggtcc atacccccac gctaggcgga tcgtggtgca
     8701 gcgtccggcg atggggtcgc tcgagcagcc aagatgccgg acgtcgtgcg tggcctcggt
     8761 gttgcgtccg gcgatgtggt cgcttgagca gccaaggtgc cggacgttgt ggcaccgggc
     8821 gagaaccttc cgcggggcgg ctggaagaat cagcgccatg cccgatgcgc ccgcgcccct
     8881 gaccctgccc ggctacgccc acgtgtactc cggcaaggtg cgcgacctct acgcgccggt
     8941 cgacccggca tccggtgagc cgcgccccga ccagctgctg ctcgtcgcgt ccgaccgcat
     9001 ctcggcgttc gacttcatcc tcgacagccc gatccccgac aagggtgagg tgctgacccg
     9061 cctgtcgctg tggtggttcg cccagctcga ggacctcgtg cccaaccacg tcatctccac
     9121 cgacgtcccg gatgccgtgc gcggccgcgc cgtgctcgtc gagcgtctcg agatgctccc
     9181 cgtcgagtgc gtcgcccgcg cgtacctcac cggcggtggg ctgggggagt acgccgcgac
     9241 cggtgccgtg agcggcatcg cgctgccggc ggggctggtc gacggcgacc ggctgcccga
     9301 gccgatcttc accccctcga ccaaggcccc gatgggtgag cacgacgagc cgatcccgtt
     9361 cgctgccgtc gaggccgaga tcggctccga cctcgcggcc cgggtgcgcg acctcaccgt
     9421 ggcaatcctc aagcgtggca acgagatcgc cgcggcgcgt ggcatcctgc tcgccgacac
     9481 caaggtcgag ttcggcctgc gtggtgacga ggtcgtgctc gccgacgagg tgctcacccc
     9541 cgactcgtcg cggttctggc cggccgacca gtgggagccc ggccacccgc agcccagttt
     9601 cgacaagcag ttcgtgcgcg agtggctgac gtcgccggcg tccggctggt cgaagagctc
     9661 g
//
LOCUS       DAOSOC010000087         9577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7100_length_9577_cov_14.790275, whole genome shotgun sequence.
ACCESSION   DAOSOC010000087 DAOSOC010000000
VERSION     DAOSOC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7100_length_9577_cov_14.790275"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..409)
                     /locus_tag="PL014_07850"
     CDS             complement(101..409)
                     /locus_tag="PL014_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57039.1"
                     /translation="MSDVRLSVTQLDPQQLGRPGTILLDVIEDDATVAERAIAWATVR
                     GYDRLVPALQSILGGPAPESGFAAVINKPHSLLVFATWPWCPTPHAVEGAPSTEWPDR
                     "
     gene            complement(539..841)
                     /locus_tag="PL014_07855"
     CDS             complement(539..841)
                     /locus_tag="PL014_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57040.1"
                     /translation="MSSIDVIAYALTIAALAALALLGLRVWRQTPERVTTRGLAGTAA
                     LTAVSALLAGYLLTMAQPFPVHVGLIIYARVSVLAAVALAGLAIAQATRSTRRADS"
     gene            complement(844..1677)
                     /locus_tag="PL014_07860"
     CDS             complement(844..1677)
                     /locus_tag="PL014_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57041.1"
                     /translation="MTEQQQSPGLDPAEAYATEYRRLWDATVATLTAAVQLDHPQHGA
                     VDFSDFLASALGAVAANVGSVGRITAGRSGSWESAALGQLVGGTVGYDADAVELAPRR
                     TVPVLVPLNVAQLVTEAYQDAQAESREAMLPHVDDAAQVIYQAGEQWRAEHPAPAEDA
                     PEAEWETYSAAEDAQIEQEQADEDVLRARYFATFEAYAEAFTAAVLDEARAIEGLSVP
                     VEVQVQTDPEAGWWGTTDTTNPVDDEYQSDPLAWRLWSAARDRVPVPSVLVEIPAEAE
                     E"
     gene            complement(1740..2366)
                     /locus_tag="PL014_07865"
     CDS             complement(1740..2366)
                     /locus_tag="PL014_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57042.1"
                     /translation="MPQKRRTLGDRNRASIALDPTPELEPSAEPRSSAQPIPAATPVK
                     APQKPRTAPSTGSTPRTPAPARKTATAAASDTARLGIYLTPEEFDDAKAGYLAAWSNG
                     GEADTFGKWIAAAIEAYAARTPKQRAAAPARGRAEERTGSTRSFAIPSDTVGRMRAAI
                     TADQKAGRWPSDSAWCGEAIAAAVDQAREQNGGTLPTPPPRLPNRLVR"
     gene            complement(2402..3193)
                     /locus_tag="PL014_07870"
     CDS             complement(2402..3193)
                     /locus_tag="PL014_07870"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOT57043.1"
                     /translation="MTIYAVALSKGGSTKTTTAAELVAALARRGRRVLAIDLDQQGNL
                     TTRLGVTDDTEVSAVAADVLTAEATAPGAAIDSPSVPGASILAGTHDLASLDQRPEVI
                     TALRDHLPEVAGEWDDVVIDTPPALGLVTLAALAAADVVVAAVACETEAYDQLDRLVE
                     VIAQRIAPRMKPGQRVHWIVPTRYDSRRLLDRDVVDMLTEQHPGRVTAPVREAVAARD
                     AYTSGMPVSVFAPGSGIAADYAAALAPIIGTPEPVGTSTEQHANA"
     gene            complement(3294..3698)
                     /gene="ssb"
                     /locus_tag="PL014_07875"
     CDS             complement(3294..3698)
                     /gene="ssb"
                     /locus_tag="PL014_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT57044.1"
                     /translation="MSSITFAGNLAADPELRFTPSGRAVARFTVIENRRRRTEDGQGW
                     EDAEPNVFRVQVWGSFAENVVESCGKGDRVHVTGSIVTDRWMDKETREDRTAQHVKAD
                     EVSFSLRYHTVRATKATRSNSQAPEAEDTHEG"
     gene            complement(3765..4031)
                     /locus_tag="PL014_07880"
     CDS             complement(3765..4031)
                     /locus_tag="PL014_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57045.1"
                     /translation="MDQQHTPPTLAEVQGTLDELAAAARAGEADRYRAALRVAKAQDL
                     TEEQISDAYRWGRRHEGAAAFDWEGTTCCGENRPKNIARPTRQH"
     gene            complement(4147..4389)
                     /locus_tag="PL014_07885"
     CDS             complement(4147..4389)
                     /locus_tag="PL014_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57046.1"
                     /translation="MKARSATVGSTRVTYAGRKGFTIHGRAKGGRWVREAWFARAGSV
                     DAYAAGLGIAYGQEVREAQAKAIERWDQQASEQVGG"
     gene            complement(4386..4565)
                     /locus_tag="PL014_07890"
     CDS             complement(4386..4565)
                     /locus_tag="PL014_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57047.1"
                     /translation="MIYAGDETLSDQQQNPELDDEGDPIDYPCEECGADAGEECRPYC
                     TGVPGRAAEAEGDER"
     gene            complement(4562..4846)
                     /locus_tag="PL014_07895"
     CDS             complement(4562..4846)
                     /locus_tag="PL014_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57048.1"
                     /translation="MTWSPTISSDRREAIGRASRAVVRLAIDPEAKEPAHRQNVIVND
                     VQAFSDREAWDAVAALEHMVLSRGLVTAQQVEAERRAVAVAVLMGEEVAR"
     gene            complement(5195..5434)
                     /locus_tag="PL014_07900"
     CDS             complement(5195..5434)
                     /locus_tag="PL014_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57049.1"
                     /translation="MTRLTVTRAADLQAGDRIVSVGPTITYRTPRKVAAALAPIAPGS
                     PVLGVRLVNPDPTSPLELVLYPSQVDGLALVIERD"
     gene            complement(5579..6673)
                     /locus_tag="PL014_07905"
     CDS             complement(5579..6673)
                     /locus_tag="PL014_07905"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOT57050.1"
                     /translation="MSKVDHKATRQAMTEQAAAFIANPAGWPAAMAARALRLRAGHPA
                     YSPNNHALIVGQLWGRFAAEGMTEDAAFSAAMHAAADEIAPRYIWARRGYTPTGGALC
                     VWSKPLPMWVDPISGEKVSKDTPGAEERRVFRVEETYRAVDVTNDQGETGAAAFAAPE
                     LPEGKAREVFELLAAWITGQGWTVQRSGREMAEGGYTSHATRQIVVHGGLAEWAAVET
                     LAHEIAHALLHGADDERPYAGEHRGDMEAEAEAVAFGLLTAFGQGELARGSARYAAEW
                     TRNPERVAVAYERSCHVLDAVAAVAFGAQDVTLTRSAKAEKAQAKADNKALAAALREA
                     GLEPKGEAWQRAKAGESIEAIAADLLDVAA"
     gene            complement(6800..7132)
                     /locus_tag="PL014_07910"
     CDS             complement(6800..7132)
                     /locus_tag="PL014_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57051.1"
                     /translation="MSTTTRSHHRCAVDECTNPTRGDLIRAHGGRWHLIDGFVYSEDR
                     RAFVGDSWAQACEEHRDTVAQAVRADVEGWRDRWSVQVRTLDYSHAEASAVIEYAQGI
                     GTDDGRLF"
     gene            complement(7486..8472)
                     /locus_tag="PL014_07915"
     CDS             complement(7486..8472)
                     /locus_tag="PL014_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57052.1"
                     /translation="MPVWSSAADWLDRLSAALRTPEGEEKRAAAKVSADTLIDVAAAD
                     ARAADSRTGRGVATAHDTVAKALGCSGKTVQRARLLIEALGFAKTVIVGRYLTTEERA
                     EAHAHHGGDQRRMASERALVVPRHVSNVHLPPWGCFNSHLPTRSDLPKRASARSGAAS
                     RQPRTTKSPKRCQPRPTIAVQRLAAALAARLPWLARGHIGSLCRTLTVLGLDDTGWSA
                     SDVIDMLDRRNVQLGLYSLTGGSQRDPLALFAHQVRAALVDVDEPPRLRRAREAAARA
                     AARAAARAEREAEDARLEAQRNDPAVQARIAAIKAQMREQLAAARSQARYRR"
     gene            complement(9157..>9577)
                     /locus_tag="PL014_07920"
     CDS             complement(9157..>9577)
                     /locus_tag="PL014_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOT57053.1"
                     /translation="LDAVHAVAAKYYRLIFMDSGNDEGDALWLRMIDHADQIVVATTT
                     RPDHAEAGRLLLNALAERDEHSARLADQAVVLVSQADREEAQAASIAQGFDALARAVV
                     TVPYDPAMRQQWLRVDNLAATTQRAYLRAAAAVAAGL"
BASE COUNT         1122 a         3141 c         3614 g         1700 t
ORIGIN      
        1 ctgaccccgc cacgaagcga ccgaagaacc ccgccattca gcagacgaag tcacacgcgg
       61 gtcgtcgacg tcctcgacga tggccgcccg taatcggtcg ttaccggtcg ggccactccg
      121 tgctcggcgc gccctcgacg gcgtgaggcg tggggcacca cggccaggtg gcgaacacga
      181 gcagcgaatg tggcttgttg atcactgcgg cgaacccgct ctcgggtgcc gggccgccga
      241 ggatgctctg cagggccggg acgagccggt catagccgcg cacggtggcc caggcgatcg
      301 cgcgttcggc cacggtggcg tcatcctcga tcacgtcgag caggatcgtg cccggcctgc
      361 ccaactgctg cgggtcgagc tgcgtcacgg acagacgaac atcactcacg gttgctctcc
      421 ttcgggtcgc tggtccgggg cttgtgctga caggcgcagt gccgtcaggg gtactcgcgg
      481 cctgcctggg tgtcgatgca gtcggccgcg tcgtggggct ctcggcacgg gtcgcagatc
      541 acgagtcggc ccgcctcgtc gacctggtgg cctgggcgat cgccagcccg gccagggcca
      601 cggcggctag gacgctcacg cgggcgtaga tgatgagccc gacgtgcacg gggaacggct
      661 gggccatcgt gagcaggtag cccgcgagca gggcgctgac ggccgtcaag gccgcggtgc
      721 ccgccaggcc tcgcgtggtg acccgctccg gggtctgccg ccacaccctg agcccgagca
      781 gggccagcgc ggccagggcc gcgatggtga gcgcgtaggc gatcacgtcg atgctggaca
      841 tgctcactcc tcggcctcgg ccgggatctc gacgagcacg ctcggaacgg gcacccggtc
      901 acgagctgcc gaccacaacc gccaggcgag cgggtcactc tggtactcgt cgtcgacggg
      961 gttcgtggtg tccgtggtgc cccaccaccc cgcctcggga tcggtctgca cctgaacctc
     1021 gaccggcacc gacagtccct cgatggcgcg ggcctcgtcg agcacggcag cggtgaaggc
     1081 ctcggcgtag gcctcgaacg tggcgaagta ccgggcgcgc agcacgtcct cgtcggcctg
     1141 ctcctgctcg atctgggcgt cctcagccgc tgagtaggtc tcccactccg cctcgggcgc
     1201 atcctcggcc ggggccgggt gctctgcccg ccactgctcc cccgcctggt agatcacctg
     1261 ggcggcgtcg tcgacgtgcg gcagcatcgc ctcgcggctc tcggcctggg cgtcttggta
     1321 ggcctcggtg acgagctgcg cgacgttgag cggcacgagc accgggacgg tgcgccgtgg
     1381 tgccagctcc accgcgtcgg cgtcatagcc cacggtcccg ccgacgagct gcccgagcgc
     1441 ggctgactcc cacgagccgg accgtccggc cgtgatccgc ccgacgcttc cgacgttggc
     1501 ggccacggct cccaacgctg aggcgaggaa gtcggagaag tcgaccgctc cgtgctgcgg
     1561 gtggtcgagc tgcacggcgg cggtgagggt ggccacggtg gcatcccaca gtcggcggta
     1621 ctcggtggcg taggcctcgg ccgggtcgag gccggggctc tgctgctgct cggtcatggt
     1681 gtgtgtgtcc tgtctgccgt gtcgagttgg tcaatgctgt cagcgtggtc tgacatgggt
     1741 tagcgcacga gccggttggg caggcgcggc ggcggggtgg ggagcgttcc gccgttctgc
     1801 tctcgggcct ggtcgacggc ggccgcgatg gcctcgccgc accatgcgct gtccgagggc
     1861 caccgtccgg ccttctggtc ggcggtgatc gcggcccgca tccgtcccac ggtgtcgctg
     1921 gggatggcaa acgacctggt ggacccggtg cgctcctcgg cgcggcccct ggccggtgca
     1981 gctgctcgct gcttgggtgt gcgggctgcg taggcctcga tcgcggccgc gatccactta
     2041 ccgaacgtgt cggcctctcc cccgttgctc cacgcggcca gatagccggc ctttgcgtcg
     2101 tcgaactcct cgggggtgag gtagatgccg agcctggcgg tgtcgctggc ggccgccgtg
     2161 gcggtcttac gggccggtgc gggcgtgcgg ggcgtgctgc ccgtgctcgg cgctgtgcgg
     2221 ggcttctgag gggccttgac gggcgtcgcg gccgggatgg gctgtgccga gctgcgcggc
     2281 tcggccgagg gctcgagttc cggggtcggg tcgagggcga tcgaggcgcg gttgcgatcg
     2341 cctagcgtgc ggcgcttctg cggcatggcg ttggtctcct tggtctggtt ggtcgatgtg
     2401 gtcaggcgtt ggcgtgctgc tcggtgctgg tgccgaccgg ctcgggcgtc ccgatgatcg
     2461 gtgccagggc ggcggcgtag tcggccgcga tccctgagcc gggcgcgaac actgagacgg
     2521 gcatccccga cgtgtaggcg tcgcgggccg cgacggcctc gcgcacgggg gcggtgacgc
     2581 ggccggggtg ctgctcggtc agcatgtcca ccacgtcgcg gtcgagcagg cggcgggagt
     2641 cgtagcgggt gggcacgatc cagtgcaccc gctggccggg cttcatcctc ggggcgatcc
     2701 gctgggcgat cacctccacg agccggtcga gctggtcgta ggcctcggtc tcgcacgcga
     2761 cggcggccac cacgacgtcg gcggccgcca gggcggccag ggtgaccagg ccgagtgcgg
     2821 gcggggtgtc gatcaccacg tcatcccact ccccagccac ctcgggcagg tggtcgcgta
     2881 gcgccgtgat cacctcgggc cgctggtcca gactggccag gtcgtgggtg cccgcgagga
     2941 tgctcgcgcc gggcacgctc ggggagtcga tcgcggcccc gggcgcggtg gcctcggcgg
     3001 tcagcacgtc ggcggcgacg gctgacactt cggtgtcgtc ggtgacaccg agccgggtcg
     3061 tgaggttgcc ctgctggtcg aggtcgatcg ccagcacgcg gcggccgcgc cgggccagcg
     3121 cggcgacgag ctcggcggcg gtcgtggtct tggtcgagcc gcccttactg agggcaacgg
     3181 cgtagatggt catgctcggg ttctccttgg tctggtggtc tgcgcgtcgg tggtcctcat
     3241 gcgacgggcg ggggcggccg gtgtggcctc tcccccgccc tgtcgacgag gggtcagccc
     3301 tcgtgggtgt cctcggcctc gggggcctgg ctgttgctcc gggtggcctt ggtggcgcgc
     3361 accgtgtggt agcgcaggct gaacgacacc tcgtcggcct tgacgtgctg ggcggtgcgg
     3421 tcctcgcggg tctccttgtc catccaccgg tcggtgacga tgctgccggt gacgtgcacg
     3481 cggtcgccct tgccgcagga ctcgacgacg ttctcggcga acgatcccca gacctggacg
     3541 cggaacacgt tgggctcggc gtcctcccaa ccctggccgt cctcggtgcg gcggcggcgg
     3601 ttctcgatga cggtgaaccg ggccacggct cggccgctgg gcgtgaaccg cagctcgggg
     3661 tcggcggcga ggttcccggc gaaggtgatg ctggacatgt cgtgctcctt ggtggttgtg
     3721 gcggttggtc cgccgtgttg atagtgccag tctagtctag ttggtcaatg ctgtctagta
     3781 ggtcgggcga tgttttttgg tcgattctca ccacagcatg tcgtgccctc ccagtcgaac
     3841 gcggccgctc cctcgtggcg gcggccccat cggtaggcgt cgctgatctg ctcctcggtg
     3901 aggtcctggg ccttggccac gcgcagcgcg gcgcggtagc ggtcggcctc tccggctcgg
     3961 gcggcggccg ccagctcgtc gagggtgcct tgcacctcgg ccagggtggg cggggtgtgc
     4021 tgctggtcca tctgctgtcc tcctgctgct cgtagactgg tggtgtgcgt ccggcgtggt
     4081 aaccggtcgc cgtgttgatg ccgtcccccc ggctcgctct cgtggcgtca ccggggggac
     4141 ggtgctttat ccccctacct gctcgctggc ctgctggtcc catcgctcga tggcctttgc
     4201 ctgggcctcg cggacctcct ggccgtaggc gatgccgaga ccggcggcgt aggcgtcgac
     4261 gcttccggcc ctggcgaacc acgcttcgcg gacccaccgg ccgcctttcg cgcggccgtg
     4321 gatggtgaag cccttgcgtc cggcgtaggt gaccctggtc gacccgactg ttgcgctgcg
     4381 ggccttcatc gctcgtctcc ctcggcctcg gccgcgcgac ctggcacgcc tgtgcagtag
     4441 gggcggcact cctcccccgc gtcggctccg cactcctcgc acgggtagtc gatggggtcg
     4501 ccctcgtcgt ccagctcggg gttctgctgc tggtcgctca gggtctcgtc gcctgcgtag
     4561 atcaccgcgc cacctcctcg cccatgagca cggccacggc gacggcgcgg cgttctgcct
     4621 cgacctgctg ggcggtgacg agcccacggc tgagcaccat gtgctctagc gcggccacgg
     4681 cgtcccaggc ctcgcggtcg ctgaacgcct gcacgtcgtt gacgatcacg ttctgccggt
     4741 gcgctggctc cttggcctcg gggtcgatgg ccagccgcac gacggcccgg cttgctcggc
     4801 cgatcgcctc gcgccgatcg ctgctgatgg tcgggctcca cgtcatgacg tcctccttgg
     4861 tgtggtgggg gtggtaggtc cccggttggt ctgccgtgtt gatatggtca gtctagtcta
     4921 gttggtcaat gctgtctagt aggtcgggcg ctttttcttg gtcgatcttc gacgtgctgc
     4981 gcgcggttgg tgacgcttgg ccctctctcc ccccgtcccc tgatagaggc cgagaatgtc
     5041 gccgggagtg gtgctgtcca gggtggccgc caggccatcg cgcagcgacg cgcagcgccc
     5101 ttgaccgcac cgggaccggc gcgagaatgg aaggcctcgg gggcggggga gtggtgagcc
     5161 gggagactga cccggcccac ctgcccacct gttcctagtc gcgctcgatg acgagtgcga
     5221 gcccgtcgac ctgcgagggg tagagcacca gctctagcgg gctggtcggg tcggggttga
     5281 ccaggcgcac gccgagcacg ggtgagccgg gcgcgatcgg ggcgagggcg gccgcgacct
     5341 tgcgcggggt ccggtaggtg atcgtcgggc cgacgctgac gatgcgatcc ccggcctgta
     5401 ggtcggcggc gcgggtaacg gtgagtcggg tcatgggcgg tgctccttgg tgtggtgggg
     5461 gtggtaggtc cccggttggt ctgccgtgtt gatatggtca gtctagtcta gttggtcaat
     5521 gctgtctagt aggtcgggcg tgggaaagcc ggggatttct ccccggctcc cttcgctgtc
     5581 aggccgcgac gtcgagcagg tcggcggcta tggcctcgat ggactcgccc gccttggcgc
     5641 gctgccaggc ctcgcccttg ggctctagtc cggcctcgcg cagcgcggcg gctagggcct
     5701 tgttatcggc cttggcctgg gccttctcgg ccttggccga gcgggtgagg gtgacgtcct
     5761 gggcaccgaa cgcgacggcc gccacggcgt cgagcacgtg gcacgaccgc tcgtaggcca
     5821 cggcgacccg ctcggggttg cgggtccact cggcggcgta gcgggccgat cctcgggcca
     5881 actcgccttg accgaacgcg gtcagcagcc cgaacgcgac ggcctcggcc tcggcttcca
     5941 tgtcgccccg gtgctcgcct gcgtagggcc gctcatcgtc ggcaccgtgc agcagggcgt
     6001 gcgcgatttc gtgggccagc gtctcgacgg ccgcccactc ggcaagtcct ccgtgcacga
     6061 cgatctgccg ggttgcgtgg ctggtgtagc caccttcggc catctcgcgg ccgctgcgct
     6121 gcacggtcca gccctggccg gtgatccacg cggccagcag ctcgaacacc tcgcgggcct
     6181 tgccctcggg cagctcgggg gcagcgaacg cggccgctcc ggtctcaccc tggtcgttgg
     6241 tgacgtcgac ggcgcggtag gtctcctcga cgcggaacac gcggcgctct tcggctccgg
     6301 gggtgtcctt gctgaccttc tcgccgctga tcgggtcgac ccacatcggc aacggcttgg
     6361 accacacgca cagcgcgcca ccggtcgggg tgtagccacg gcgtgcccag atgtagcgcg
     6421 gggcgatttc gtcggcggcc gcgtgcatgg cggcgctgaa cgcggcgtcc tcggtcatgc
     6481 cctcggcggc gaaccgtccc cagagctgac cgacgatcag ggcgtggttg ttgggggagt
     6541 aggcggggtg accggcgcgc agccgcagcg cccgggcggc catcgcggca ggccatccgg
     6601 cggggttggc gatgaatgcg gcggcctgct cggtcatcgc ctggcgggtg gccttgtggt
     6661 caaccttgct catggtgtcc tcctcgtggt gggggtggta ggtccccggt tggtctgccg
     6721 tgttgatatg gtcagtctag tctagttggt caatgctgtc tagtaggtcg ggtggttttc
     6781 ttcggggaat cttggcgggt cagaacaggc ggccgtcgtc ggtgccgatg ccctgggcgt
     6841 actcgatgac ggctgaggcc tcggcgtggc tgtagtcgag cgttcggacc tggacgctcc
     6901 accggtcgcg ccagccctcg acgtcggccc gcacggcctg cgccacggtg tcgcggtgct
     6961 cctcgcacgc ctgcgcccat gagtcgccca cgaacgcgcg ccggtcctcg ctgtagacga
     7021 atccgtcgat caggtgccag cggccgccgt gggctcggat gaggtcgccg cgtgtggggt
     7081 tggtgcactc gtcgacggcg catcggtggt ggctgcgggt ggtcgtgctc atcgtgtctc
     7141 cttggtgtgt ttgggctagt tggtcaaaag gtgagacggg aggccaaccc ggcaccgtgc
     7201 tcttggcatc gccgctgacc actttttcct cccaccccct gacgtttgtc cccgtcgcgg
     7261 ccgcataagg gtcaccgggt caacccgaag ggcggtgggg gagagaagtt ttcgggcgaa
     7321 ataagcggag ggaggaacga ccggagcgcg tccgaaaagt tctcggagga accggcgcgc
     7381 agcgcttgac gcggtggccc gtggccgcag agtgggacgt cagggggagg gagtaggtga
     7441 ccttccgtga ccgagtcttg gaccgcctgc ctgcttgtcg gcgcgttagc gccggtatcg
     7501 ggcctggctg cgcgcggccg ccagctgctc gcgcatctgc gccttgatgg ccgcgatccg
     7561 ggcctggacg gccgggtcgt tgcgctgggc ttccagacgg gcgtcctcgg cctcgcgttc
     7621 ggcccgagca gctgcacgcg ctgctgccct ggcggccgcc tcgcgggcgc gccgtaggcg
     7681 cgggggctcg tcgacgtcga cgagggctgc tcgcacctgg tgggcgaaca gcgccagcgg
     7741 gtcgcgctga gagccgccgg tcaggctgta gagcccgagc tggacgttgc gccggtcgag
     7801 catgtcgatg acgtcgctgg cgctccatcc ggtgtcgtcg aggccgagca cggtcagcgt
     7861 gcggcacagg gacccgatgt ggcctcgtgc gagccacggc aggcgcgccg ccagggcggc
     7921 cgcgagtcgt tgcaccgcga tcgtgggccg tggctggcag cgtttcgggc tcttggtcgt
     7981 ccttggctgc cgcgaagcgg ccccagagcg cgcgctagcg cgctttggta agtcactacg
     8041 tgtagggaga tgagagttaa agcaacccca cgggggtagg tggacattcg atacatgacg
     8101 ggggacgacc agggcgcgtt cggaggccat gcggcgctgg tcgccgccgt ggtgggcgtg
     8161 ggcctcggca cgctcctcgg tggtgaggta gcggccgacg atgacggtct tggcgaagcc
     8221 cagggcctcg atgagcagcc gggcgcgttg gacggtcttg cccgagcagc ccagggcctt
     8281 ggccacggtg tcgtgggcgg tggcgacacc gcgacccgtg cgggagtcgg ccgcgcgggc
     8341 gtcggcggcc gcgacgtcga tcagggtgtc cgcgctgacc ttggcagcgg cgcgcttctc
     8401 ctcgccctcg ggcgttctga gggccgctga gagccggtcg agccagtcgg ccgcgctaga
     8461 ccacaccggc acgctggcgg gaagcctggg gccgtctgtg ggccgccagg cgcggcctcg
     8521 gcacgagggc gcgatcgcgc tgcgcagatc acgcgggccg gtgcacggcc ggtagtaccg
     8581 acgcgggcgt gctagcccgc tagaaggctc gtcgacgtcg acgagctgag tccggcgagc
     8641 gagtgattca ctcgctttcg gacgtgcaag tgctatcgtt ggcatcgttc ctcgggtcga
     8701 tcgagggcat gagaaaaccg ctgagcggct tccgccgctg agtccttggc aagggactgg
     8761 ctacaaatcg gaggtccggg gttcgagccg gacgctcaat ctcggacaaa ggctcccgtt
     8821 ggcgcggggg ccttcgtcat gtctgggggt cagccggtca tgggcaactc ctgctgcttg
     8881 ctgtcggcag gacggccgga tcggcggtcc tgagcctcgg gccggtcaag cacgatgcgc
     8941 tctaggtagg cctgcatcga gactccggct gcttgggcgc gccgacgcag ctcggcgcgc
     9001 tccgaggggc tacagcggaa cgtgaccatc gcgctgcgca cctcgcttcg gtgcgtgctt
     9061 cctggtgcag acataagtgc atccttgcag tggcatcgct atctggaagt cattccctag
     9121 tcagtcggcg tgttgcgtct gtgacgagtg ggactgtcac agcccggccg cgacggctgc
     9181 ggccgcgcgc aggtaggcgc gctgggttgt ggcggcgagg ttgtcgaccc tgagccactg
     9241 ctgacgcatc gcggggtcgt aggggacggt gaccacagcg cgggccaggg cgtcgaagcc
     9301 ctgggcgatc gaggccgctt gcgcctcctc gcggtcggcc tgggagacca ggacgacggc
     9361 ctggtctgcg agacgagctg agtgctcgtc tcgctcggcc agagcgttga gcaggaggcg
     9421 tcctgcctcg gcgtggtcgg gccgggtggt cgtggccacg acgatctggt cggcgtggtc
     9481 gatcatgcgc agccacaggg cgtcgccctc gtcgttaccg ctgtccatga agatcagccg
     9541 gtagtacttc gcggcgacgg cgtgcacggc gtcgagc
//
LOCUS       DAOSOC010000088         9562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7115_length_9562_cov_9.643736, whole genome shotgun sequence.
ACCESSION   DAOSOC010000088 DAOSOC010000000
VERSION     DAOSOC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7115_length_9562_cov_9.643736"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..832)
                     /locus_tag="PL014_07925"
     CDS             complement(140..832)
                     /locus_tag="PL014_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOT57054.1"
                     /translation="MAALVAGALFSSSVRASESGDLRPEQGELADLIRAQNRTNEQYA
                     SRLNELQAKVDEATAALAPGDLETRDLEDKANKLAVEAGRTEVVGPALRVTLNDAKLP
                     GGELPDGADPDDYVIHQQDVQAVVNALWAGGAEAMMLQDQRVISTSAVRCVGNTLILQ
                     GRVYSPPYVITAIGDTGAMRDALEKDPQVENLRQWSVAVGLGYDSALVGEQTFPAFVG
                     SIVPEHAQVPTA"
     gene            962..1213
                     /locus_tag="PL014_07930"
     CDS             962..1213
                     /locus_tag="PL014_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HOT57055.1"
                     /translation="MPESKGRSKPAYTPPRSAKKDPGPNARWFVPVMLGLMLAGLIWV
                     VTYYISGVNEYPVPQLGRWNLGVGFGLMMSGFLMTTRWR"
     gene            complement(1655..2548)
                     /locus_tag="PL014_07935"
     CDS             complement(1655..2548)
                     /locus_tag="PL014_07935"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT57056.1"
                     /translation="MAEQPASPDAVAPVPVCPRHPDRESYVRCQRCMRPTCPECQRPA
                     AVGIQCVDCVAEGAKTVRQVRTVFGGAVTDGRPVVTMTLIGICVAVYLAQSSVPGFER
                     QLWFSPVLGQSEPWRFITSAFAHGSIMHIAFNMYALWIMGTYLEPMLGRARFIAIYLL
                     SAFGGSVFYLLLAAPQSYEEAVAFGPGMWWTPAVGASGAVFGLFGAFLVLQRRLGRSA
                     AGMYVIIGINAVIGFVLPNIAWQAHLGGALTGAAAAAAIAYLGRNRGPLKPVDRRVHW
                     VALGGLFVLLVVLSLAKYATA"
     gene            complement(2588..3106)
                     /locus_tag="PL014_07940"
     CDS             complement(2588..3106)
                     /locus_tag="PL014_07940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT57057.1"
                     /translation="MKATLHTNHGPIVVELFPNEAPKTVANFTGLATGEKEYKDDAGR
                     SNPTPYFDGLIFHRIIPGFMIQGGCPLGRGMGGPGYNFDDEIHPDKQFDRPYILAMAN
                     AGKRMGKGTNGSQFFITVAPTTWLNGKHTIFGEVADGPSREVVDKIAGVRTGAQDKPL
                     EDVVIERVELND"
     gene            3264..4187
                     /locus_tag="PL014_07945"
     CDS             3264..4187
                     /locus_tag="PL014_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57058.1"
                     /translation="MFRSDSKADQAKDAVTTGVASVTDSAHDAAERAAELKVKAAEAA
                     EAAAATAAAAKVKAAEAAEYAQGAAETAKEKAHHAAENLAPKVESAREVFVEDVLPKV
                     VSAIAAVAAGAAAAKDTATETAERAPDAFAVLKGDAVAKQGGGKGKWLLLLGAIAAGA
                     AIMAYRKSNERPDPWATAGTYTPPKSTSEKVGDLADKAKDKAAAASDAVSGAVSDAKD
                     KAADAKDAVAEKVADVKDAAGDAAHDAKDAASDAVDTAGDAVSDAGDTASDAIDDAKD
                     AASDAADAAGDAAHDTNDAASDAVDEANRKS"
     gene            4393..5739
                     /locus_tag="PL014_07950"
     CDS             4393..5739
                     /locus_tag="PL014_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57059.1"
                     /translation="MNDAMRVRDIPAVLIAAARILGRFWAPFIVIACLGLALRSGALW
                     VAVEVSDHNAFVAQLILTIAPLGFLLAMIAMLYLTRASLPNVAALSHRKARQATTEKR
                     EMRLVDVAVSVLVPFLAVYVSYGLLQQDLYRFTNEAAFDEFNQFSLDGSTEYDYAGRL
                     AIYDWQVVVAIVAIAWVLRFALGRLERVTQFLALAFVGALVEVYYTSQVAQKVSDLRA
                     ASIEWVQQRQASQVVVEQYDRVVDELGPLANPVDSATGWLFGLIGAVDAVVVVPLAWL
                     TVGAVVLGHRLSPPPKPTREPNPTLQKIPAPVRRFGGGLVADVRERWSAFWNGLRMMA
                     AAGLMPMLTFCLAFLLVIRIPWALGEVLRLAIGPVNTDTWLAFAPMEAALGLAVTMVL
                     AASLLAAAIDWLLGPSLDPTPEPDEPSDDSTAEPTEAPTAEPTASGEPEISVPASA"
     gene            complement(5717..6346)
                     /locus_tag="PL014_07955"
     CDS             complement(5717..6346)
                     /locus_tag="PL014_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57060.1"
                     /translation="MIGARPSRWWILAIPFAIALMLAASGYRVGTFWFHNGQHRVLAS
                     ADAGEWARASEDYTDVFGDTTRSYAVRLAGWGQESSTFVNSRGEEFALPDGMVARTVN
                     LEFEAEPDQALTSCTISLIDDQGRRFDNGGSTSQVANESSACVPEETPGPSLAVLDTD
                     VRGATPDDTQPRPTRWATSPQVVVPRDAEIVEVRVSFEHPDYVRLTPER"
     gene            complement(6343..6960)
                     /locus_tag="PL014_07960"
     CDS             complement(6343..6960)
                     /locus_tag="PL014_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57061.1"
                     /translation="MNRAASTAATAAFVIAAMGLGRLITDHVPLEASAVEPFTVTAGL
                     GQTAALEYAEVTVTGIRVTPTITGNPTAKAGGRWLVVDTELVATREPTLMLGFDLVAA
                     DGTRYAHSSRGTDCAINATLATGVPWYGSFCFDVPEAALDDPHFLVSRGDYAVDGSGQ
                     RRDGLADIDLAIGPADVGTLWSRTEPVDLSPTGVVPPAPAEQAAP"
     gene            complement(6968..7585)
                     /locus_tag="PL014_07965"
     CDS             complement(6968..7585)
                     /locus_tag="PL014_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57062.1"
                     /translation="MGDRPSVRAVLSRARTFAAARPAKQKAGAAAAGVLLVSAPFGGL
                     AAAPEPPPQQLLLGQPMTVGPFEVAIDKVVELPDLAPAITPEPTQRVVVVDAVVTLTG
                     DRPEYAVTLTNNLRVTGGGVKTTERPSLYVVDDATAMSVFNPGVSYRLAIAFVTDGPW
                     QGETATVRANQVVFREEDRGTLNPNAWVGLDEIQWQGSLPLERRS"
     assembly_gap    7734..7833
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8037..8330
                     /locus_tag="PL014_07970"
     CDS             8037..8330
                     /locus_tag="PL014_07970"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT57063.1"
                     /translation="MLALDVDRERLRMVCERYGVAALEVFGSVARGESRIDSDVDLLF
                     VLKPGTRLGFRLFDLEDELSALFGRRVDLVARQSINKYLREQILADAQPFYAA"
     gene            8320..8685
                     /locus_tag="PL014_07975"
     CDS             8320..8685
                     /locus_tag="PL014_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT57064.1"
                     /translation="MQRELLLLREMRDAATAIRELVDGRSSEQVGADGIRRSALLWHF
                     TVLGEAASQVPSGTKDANPKIAWRAATRLRNRIVHGYWDIDVETLVATAVDDLPDMIT
                     QLESAIASLQEPEGTDPAL"
     gene            complement(9314..>9562)
                     /locus_tag="PL014_07980"
     CDS             complement(9314..>9562)
                     /locus_tag="PL014_07980"
                     /inference="COORDINATES: protein motif:HMM:NF039765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6416 domain-containing protein"
                     /protein_id="HOT57065.1"
                     /translation="LMDNPGQEFTGKAIAKTLDIPNGRHGVAGVLAHPGRFAYKVQRP
                     LPSDWRYDEASGESLYWMDEGIADIFKAARAEVEAGKV"
BASE COUNT         1451 a         3237 c         3371 g         1403 t
ORIGIN      
        1 tcgaggctgt cgacgatctg ggtctgcgcc cggttggaga ccacgtcggt ccaccacagc
       61 tgccaggccg cgaacagcag cacgagcacc ccgagcgtga cgaggagttc accggcccag
      121 ccggcgacac ggcgcacgat cacgcggtgg ggacctgggc gtgctcgggg acgatggagc
      181 ccacgaaggc cgggaaggtc tgctcgccga ccagggccga gtcgtagccc agacccacgg
      241 cgaccgacca ctgccgcagg ttctcgacct gggggtcctt ctcgagggcg tcgcgcatgg
      301 cgccggtgtc gccgatcgcg gtgatgacgt agggcgggga gtacacccgg ccctggagga
      361 tcagggtgtt gccgacgcac cggaccgcac tcgtggagat cacccgctgg tcctggagca
      421 tcatcgcctc ggcgccgccg gcccacaggg cattgacgac tgcctggacg tcctgctggt
      481 ggatgacgta gtcgtcgggg tcggccccgt cgggcagttc accgccgggc agcttggcgt
      541 cgttgagggt gacgcgcagg gcagggccga cgacctcggt gcggcctgct tcgaccgcga
      601 gcttgttcgc cttgtcctcg aggtcccggg tctcgaggtc acccggggcc agcgccgcgg
      661 tcgcctcgtc gaccttggcc tgcagctcgt tgagccggga ggcgtactgc tcgttggtgc
      721 ggttctgggc gcggatcagg tcagccagct caccctgctc ggggcgcagg tctccactct
      781 cggaggcccg cacgctggag gagaagaggg cgcctgccac gagggcagcc accggcacca
      841 gccaaggcca cggagagggt cggtcgaccc gtccgccgtg tgtaccgggc atgctgtcaa
      901 cctcctctcg ggaacaccct ctacgctagg ccaccacatc aacgccacga ggagacagtt
      961 cgtgcccgag tccaagggtc gctccaagcc cgcctacacg ccaccccgtt ccgcgaagaa
     1021 ggatccgggg cccaacgcgc gctggttcgt gcccgtcatg ctcgggctca tgctggcggg
     1081 gctcatctgg gtcgtcacgt actacatctc cggggtcaac gagtaccccg tgccgcagct
     1141 cggccggtgg aacctcggtg tcggcttcgg cctgatgatg tccggcttcc tgatgaccac
     1201 gcgctggcgc tgaccgacgc gccacccggg cacgcacgcc ggtgctgacg gacgcgccac
     1261 cccggcgctg accacagatc cctgttgtcc ccagatcgac caataggtcg acctggggac
     1321 aacgtgtgtc tgcaggagga gcgatcaggc cgtgccggcc gcgctcacgc ccggactggc
     1381 ggcatccggc cgaagttatc cacaggccct gtccacactg gggacgaatg acacccgtgt
     1441 aatccacagc ccggggcagc cgatcaagcc gcaacccccg acctcaatgg agcctgtcta
     1501 gacgcagatc cttgctccgg tcgcagatcc ttactccggt ggtgcgtcct tgcagcacat
     1561 cccaaccgga gcaaggactc acatcgggag caaggatctg cgacgagcac gccgcagcgc
     1621 agcccggatc agccgcggca cacctggtgg atgatcaggc ggtggcgtac ttggccagcg
     1681 acagcacgac gagcagcacg aacaacccgc cgagcgccac ccagtgcacc cgtcggtcga
     1741 cgggcttgag cggcccgcgg ttgcgcccga ggtaggcgat cgccgctgcg gccgcggccc
     1801 cggtgagcgc cccaccgagg tgggcctgcc aggcgatgtt cggcagcacg aacccgatga
     1861 ccgcgttgat gccgatgatg acgtacatgc ccgcagccga gcgccccaga cgacgctgga
     1921 gcaccaggaa ggcaccgaac aggccgaaca ccgcgcccga ggccccgacc gcaggggtcc
     1981 accacatgcc cgggccgaac gccacggctt cctcgtagct ctgcggcgcc gccagcaaca
     2041 ggtagaagac cgacccaccg aacgcgctca gcaggtagat ggcgatgaac cgggcccggc
     2101 cgagcatcgg ctcgaggtag gtgcccatga tccacagcgc gtacatgttg aacgcgatgt
     2161 gcatgatcga gccgtgggcg aacgccgacg tgatgaagcg ccacggctcg gactgtccga
     2221 gcaccggcga gaaccagagc tgccgctcga acccgggcac cgaggactgc gccaggtaca
     2281 cggcgacgca gatcccgatg agcgtcatcg tcacgacggg gcggccgtcg gtcacggccc
     2341 cgccgaagac ggtgcgcacc tggcgcaccg tcttcgcacc ctcggccacg cagtcgacgc
     2401 actggatgcc gacggcagcg ggccgctggc actcggggca ggtcggccgc atgcaccgct
     2461 ggcagcgcac gtacgactcc cgatcggggt ggcgagggca caccggaacc ggtgccacgg
     2521 catccgggga tgccggttgc tcagccacgt cgggactccc gcctcgcgcg ctcggtcacg
     2581 accagcgtca gtcgttcagc tcgacgcgct cgatgacgac gtcctcgagg ggcttgtcct
     2641 gggccccggt gcggacgccg gcgatcttgt cgacgacctc gcggctcggg ccgtcggcga
     2701 cctcaccgaa gatggtgtgc ttgccgttga gccaggtcgt cggggcgacg gtgatgaaga
     2761 actgcgagcc gttcgtgccc ttgcccatgc gcttgccggc gttggccatg gcgaggatgt
     2821 aggggcggtc gaactgcttg tcggggtgga tctcgtcgtc gaagttgtag cccggaccgc
     2881 ccatgccgcg gcccagcggg cagccaccct ggatcatgaa gccggggatg atgcggtgga
     2941 agatcaggcc gtcgaagtac ggggtcgggt tgctgcgacc cgcgtcgtcc ttgtactcct
     3001 tctcgccggt cgcgagaccg gtgaagttgg cgacggtctt gggcgcctcg ttcgggaaga
     3061 gctcgacgac gatggggcca tggttcgtgt gcagggttgc cttcatgccg tcgatcctct
     3121 cacgacgtcc tgacggtgcc tgctcgcccg tcgcccgacg acgcgggcca tcagccgaca
     3181 gatcaggctg cacccaccat cgcgtcgagg tggggccaca gggcaagatg gtggacaaga
     3241 caacgtccac atgaggagga cacgtgttcc gttccgacag caaggccgat caggccaagg
     3301 atgccgtcac caccggggtt gcgagcgtca ccgacagcgc tcacgacgcc gccgagcgcg
     3361 cagccgagct gaaggtcaag gccgccgagg cagccgaggc cgctgctgcg accgccgctg
     3421 ccgccaaggt gaaggctgcc gaggccgctg agtacgccca gggcgccgcg gagacggcca
     3481 aggagaaggc gcaccacgcc gccgagaacc tggcccccaa ggtcgagtcc gcgcgtgagg
     3541 tgttcgtcga ggacgtcctg cccaaggtcg tcagcgccat cgccgcggtc gccgccggtg
     3601 ccgccgctgc gaaggacacc gccaccgaga ccgccgagcg cgcccccgac gccttcgccg
     3661 tgctcaaggg tgacgccgtc gccaagcagg gtggtggcaa gggcaagtgg ctgctgctgc
     3721 tcggggccat cgctgccggt gccgcgatca tggcctaccg caagagcaac gagcgccccg
     3781 acccgtgggc gaccgcgggc acgtacaccc cgcccaagag cacgtcggag aaggtcggcg
     3841 acctggccga caaggccaag gacaaggctg ctgccgcgtc ggatgccgtg tccggcgccg
     3901 tctcggacgc caaggacaag gcggccgacg ccaaggacgc cgtggccgag aaggtcgcgg
     3961 acgtcaagga cgcggctggc gacgcagcgc acgacgcgaa ggacgcggcc agtgacgccg
     4021 tggacaccgc tggcgacgcg gtctcggatg ccggtgacac ggcatccgac gcgatcgacg
     4081 acgcgaagga cgcggcttcg gacgcagccg acgcagccgg tgacgcggcg cacgacacca
     4141 acgacgcggc atccgacgcc gttgacgagg ccaaccgcaa gagctgaccg ctggctgggg
     4201 ggccaggagc gcgactgatc gcgcgatcga ccccccaccc ccgcagcaca ccacgacgcc
     4261 ggccgctcct cacggggcgg ccggcgtcgt cgtgtccgtc cgcagagcgg gcatccaaca
     4321 gccgccgagt ccaggccggc ggataggctc tcgctcggca aggcgacacg ggggccagag
     4381 caagggggca gggtgaacga cgcgatgcgg gtgcgcgaca tccccgcggt cctcatcgcg
     4441 gccgcgcgca tcctgggccg cttctgggcg ccgttcatcg tcatcgcctg cctcgggctg
     4501 gccctgcgca gcggcgccct gtgggtggcc gtcgaggtga gcgaccacaa cgcgttcgtc
     4561 gcccagctca tcctcacgat cgcgccgctg ggtttcctcc tggccatgat cgccatgctc
     4621 tacctgactc gcgcgtccct gcccaacgtc gccgcgctca gccaccgcaa ggcccgccag
     4681 gccaccacgg agaagcgcga gatgcgcctc gtcgacgtcg ccgtcagtgt gctcgtgccg
     4741 ttcctcgcgg tctacgtctc ctacgggctg ctccagcagg acctctaccg cttcaccaac
     4801 gaggcggcct tcgacgagtt caaccagttc agcctcgacg gctccaccga atacgactac
     4861 gccggccggc tcgcgatcta cgactggcag gtcgtcgtcg cgatcgtcgc catcgcgtgg
     4921 gtgctgcggt tcgcgctcgg caggctcgaa cgggtgaccc agttcctcgc gctggccttc
     4981 gtcggtgcgc tcgtcgaggt ctactacacc tcgcaggtgg cgcagaaggt cagtgacctg
     5041 cgagcggcat ccatcgagtg ggtccagcag cggcaggcct cgcaggtcgt ggtcgagcag
     5101 tacgaccggg tcgtcgacga gctcgggccg ttggcgaacc cggtcgattc cgcgacgggc
     5161 tggctgttcg ggctgatcgg cgcggtggat gccgtcgtcg tcgttccgct cgcgtggctc
     5221 acggtggggg ccgtcgtcct cgggcaccgg ctcagcccgc caccgaagcc gacccgtgag
     5281 ccgaacccga ccctgcagaa gatccccgcc ccggtgcgtc gcttcggggg cgggctggtg
     5341 gcggacgtcc gcgagcgctg gtcggcgttc tggaacggcc tgcggatgat ggccgcggca
     5401 ggcctgatgc ccatgctgac gttctgcctg gcgttcctgc tcgtcatccg catcccgtgg
     5461 gccctgggtg aggtgctgcg cctcgcgatc gggccggtca acaccgacac ctggctggcc
     5521 ttcgcgccga tggaggccgc gctcgggctg gcggtgacga tggtgctggc cgcatcgctg
     5581 ctggcggcgg cgatcgactg gctgctcggc ccctcgctcg acccgactcc cgagcccgat
     5641 gagccgtccg acgactcgac tgccgaaccc actgaagccc ccaccgccga acccaccgcc
     5701 agcggcgagc cggagatcag cgttccggcg tcagcctgac gtagtccggg tgctcgaacg
     5761 acacccgcac ctcgacgatc tcggcatcgc ggggcacgac gacctgtggg gaggtggccc
     5821 agcgcgtggg ccgtggctga gtgtcgtccg gggtggcgcc gcgcacgtcg gtgtcgagga
     5881 cggccaggct cgggcccgga gtctcctcgg gcacgcacgc ggacgactcg ttcgccacct
     5941 ggctggtgct gccaccgttg tcgaatcggc gcccctggtc atcgatgagc gagatggtgc
     6001 aactcgtgag ggcctggtcg ggctcggcct cgaactcgag gttgaccgtg cgggcgacca
     6061 tgccgtcggg cagggcgaac tcctcaccgc gactgttgac gaacgtcgac gactcctgac
     6121 cccacccggc caggcggacg gcatacgagc gggtcgtgtc gccgaacacg tccgtgtagt
     6181 cctcgctcgc gcgagcccac tccccggcat ccgcgctcgc gagaacccgg tgctggccgt
     6241 tgtggaacca gaaggtgccg acccggtagc ccgacgcggc gagcatgagc gcgatcgcga
     6301 aggggatggc gaggatccac cagcggctgg ggcgggcgcc gatcatggcg cggcctgctc
     6361 tgccggggcg ggtggcacga cgccggtcgg gctcaggtcg acgggctcgg tccgggacca
     6421 cagggtgccg acgtcggcgg ggccgatggc gaggtcgatg tcggcgaggc cgtcgcggcg
     6481 ctggcccgac ccgtcgaccg cgtagtcacc gcgcgagacg aggaagtgcg ggtcgtcgag
     6541 cgcggcctcg ggcacgtcga agcagaagct gccgtaccag ggcacgccgg tggcgagggt
     6601 ggcgttgatc gcgcagtccg tgccccggct ggagtgggcg tagcgggtgc cgtcggcggc
     6661 gacgaggtcg aacccgagca tcagggtggg ttcgcgggtc gcgaccagtt cggtgtcgac
     6721 gacgagccag cgcccgccgg ccttcgcggt cgggtttccg gtgatcgtgg gggtgacgcg
     6781 gatgccggtg acggtcacct cggcgtactc cagggccgcg gtctggccga ggcccgccgt
     6841 gacggtgaat ggctcgacgg cggaggcctc gaggggcacg tggtcggtga tcagccggcc
     6901 cagacccatg gcggcgatga cgaacgcggc cgtggcggcg gtggaggcgg cgcggttcat
     6961 cgggcgctca cgaccgtcgc tccagaggga gggagccctg ccactggatc tcgtcgaggc
     7021 cgacccaggc gttggggttg agggtgccac ggtcctcctc gcggaagacg acctggttgg
     7081 cgcgcacggt ggcggtctcg ccctgccagg ggccgtcggt gacgaaggcg atggcgagcc
     7141 ggtaggacac gccggggttg aacacgctca tggccgtggc gtcgtcgacg acgtagagac
     7201 tcggccgctc ggtggtcttc accccgccgc cggtgacgcg caggttgttc gtcagggtga
     7261 ccgcgtactc ggggcgatcc ccggtgaggg tgacgacggc atccaccacg acgacccgct
     7321 gggtgggttc cggggtgatg gccggggcga ggtcgggtag ttccacgacc ttgtcgatgg
     7381 ccacctcgaa ggggccgacc gtcatcggct ggcccagcag gagctgctgc gggggcggct
     7441 cgggggcggc ggccaggccg ccgaacgggg ccgataccag gagcaccccg gcggccgcgg
     7501 cgcccgcctt ctgcttggcc gggcgcgcgg ctgcgaaggt gcgggcgcgc gacagcacgg
     7561 cgcgcacgga cggccggtcc cccatgatcg cgattctaga gggcacgttc gccggcgggc
     7621 gcccccgagc cctggcaagc tgtggggatg tcgagcccgc tgccaccgaa ccaggagtac
     7681 gtgcggcgcg agatcatcgc tgcagcattg gccacgcgag ccgaatcaac gggnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggggag cggccgatcg cgatcacgtg
     7861 gcggttgcgc gtgccgatgc ccgccgccga cttcgccact gccagcttcg tccagtagtc
     7921 ccgtgcagtg gcccctggca ctgacgcgct ctcttggacg ggcccgtgct tcaccgcgat
     7981 ccagggtcgt tcattgcggc cacggggtgc cgtgccctct ccgttagtct gggatcgtgc
     8041 tggccttgga cgttgacagg gagcgcttgc ggatggtgtg cgagcgctac ggtgtcgcgg
     8101 ccttggaggt cttcggctcc gtcgctcgag gagagagccg gatcgacagt gatgtcgacc
     8161 tgctgttcgt cctgaagccg ggtactcgat taggttttcg actgttcgac ctcgaggacg
     8221 agctgtctgc cctcttcggc cgcagggtcg acctcgtcgc gcgccaatcc atcaacaagt
     8281 accttcgcga gcagatcctc gccgacgcac agcccttcta tgcagcgtga actcctgctg
     8341 ctgcgtgaga tgcgcgatgc ggcgacggcg atccgggaat tggtggatgg tcgaagcagc
     8401 gagcaggtcg gtgccgacgg catccggcgt tcagcgttgt tgtggcactt caccgtgctc
     8461 ggcgaggctg ccagccaggt cccgtcgggg acgaaggatg cgaacccgaa gatcgcgtgg
     8521 cgtgctgcca cccggctgcg caaccgcatc gtccacggct actgggacat cgacgtggag
     8581 acgctggtcg cgacggcagt cgacgacctc cctgacatga tcacccagct cgagagcgca
     8641 atcgcctcgc tgcaggaacc tgaaggtacc gacccggccc tctgagacgg ccagcgattc
     8701 cggctggatc gtgcaacgaa ttggtgtggc ccgcggggcg ccgtgacgcc tcggcttcag
     8761 tttgacgcaa cgcggcgcaa cacggggttg atcagcctcg ccccgagcca atcgaactga
     8821 tcaaccagcc gtgggctcgt cgggccggca cgcccggttg ccctcgcaca ccgggcggcg
     8881 cgcttgtagc cgaccttgct gaggcaccgg cacccgaaga ggccctcgcc gtgagacgca
     8941 aggcctctca cctccgggct gcggtggagc caaggggact cgacccctaa ccccctgctt
     9001 gcaaaatccc cgagtggggt ccgtgagggg gtgcataagc gcaggtcagg acgctgcgag
     9061 gcagctgatc gcgccatttt cgccccccgc tgccgtactt ttctggggta cgaatctggg
     9121 gtgcaaccga ggcgggttgc ggttgcgatg aggtcgcctt ggcccggcac atcctcatca
     9181 ggcagatgag ttccccgcac cgggagcggg ccaagacgat ccgtacttgg cccgttctgg
     9241 tctagacgcc gagacacccg ccccggagag acgcagcggg tgtcttggct tgggcggcaa
     9301 gtcggccgca ggctcagacc ttcccggcct cgacctcggc tcgggcggcc ttgaagatgt
     9361 cggcgatccc ctcgtccatc cagtagaggc tctccccgga cgcctcgtcg tagcgccagt
     9421 ccgacggcag tgggcgctga accttgtagg cgaagcgccc ggggtgggcg aggacacccg
     9481 cgacgccgtg gcgcccgttg ggtatgtcga gcgtcttggc gatcgccttg cccgtgaact
     9541 cctggccggg attgtccatc ag
//
LOCUS       DAOSOC010000089         9537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7138_length_9537_cov_11.056106, whole genome shotgun sequence.
ACCESSION   DAOSOC010000089 DAOSOC010000000
VERSION     DAOSOC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7138_length_9537_cov_11.056106"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1125)
                     /locus_tag="PL014_07985"
     CDS             complement(298..1125)
                     /locus_tag="PL014_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOT57066.1"
                     /translation="MPEGHTLFALARDLHEAFAGTTPEVTSPQGKFAEGAAVLSGHEL
                     LRATSRGKHLFVEFADDRWLHVHLGLIGSFTVDSHEWTAEVPAVGQVRLRLRTDQHVA
                     DLRGPTLCEVATPEQVDTVLDRLGPDPLRPDSDPDAAWARISRSSRTIGELLMDQAVL
                     AGVGNVYRSEVLFRHRLSPFTEGRMLRRASWHLIWDDLERLMPLGVTTGRIVTVEDQV
                     LEVEAALGRGEVPRLTERSSAVYRRAGMPCPACGSKVRTRVVAGRNLFWCGRCQRRR"
     gene            complement(1118..1336)
                     /locus_tag="PL014_07990"
     CDS             complement(1118..1336)
                     /locus_tag="PL014_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57067.1"
                     /translation="MAGEHPQPTSTPEPSEPPHEVHAIGAPARRALASAGWTTWDAVA
                     STADRDLLALHGVGPRALRILRELRPHA"
     gene            complement(1339..2262)
                     /locus_tag="PL014_07995"
     CDS             complement(1339..2262)
                     /locus_tag="PL014_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOT57068.1"
                     /translation="MRAIWKGAVSFGLVNVPVRLYSATENHDVQFRQVHREDGGRIKY
                     KRTCSIDGEEVSYDDIAKGYETEDGDMVVLTDEDFADLPSRTSKEIGVTKFVPADQID
                     PMWLDKSYYLEPDKAATKPYVLLRDALETEKRMAICTVSIRTRMTMAVLRVRDGVIVM
                     QTMLWPDEVRAADFATVTDDQRATEQEMAMAKMLIDQLAGDYDPDDYEDDYAIAVKEL
                     VRAKVEGGEVRVSTAEPEESGEVVDLLAALARSVEKAKAARGEAPEPAPAKAPAAKKA
                     ASKSAATKKAAAEKAPAKKAAAKKAAPKKAS"
     gene            2446..3387
                     /gene="ligD"
                     /locus_tag="PL014_08000"
     CDS             2446..3387
                     /gene="ligD"
                     /locus_tag="PL014_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOT57069.1"
                     /translation="MRPMLATVAERVPTGADWVHEVKWDGMRVLVDVRGGEVRVWSRN
                     ERDVTASYPEFAGLGEAYDDMLLDAEVVALDGGRPSFHALTERMHVSDRRKAVRLAAT
                     RPVTLMVFDLLRLFGADLTGQPWSARRELLERLDLTGPTWQVPAVHDDGEHLLAATAE
                     QGLEGIVSKRRSATYAAGRRSPDWRKSAHKVTHSVVVGGWRPEVGSAGRLGAVLVGLP
                     DGSGGWRYAGRVGSGLAGTAGEALAVRLRPLSRDDSPFVDDVPSIDAVGTTWVQPRVV
                     IEVRALELTGQHRLRQPAYLGVRTDLVPADLEEVEDA"
     gene            3380..4264
                     /gene="ligD"
                     /locus_tag="PL014_08005"
     CDS             3380..4264
                     /gene="ligD"
                     /locus_tag="PL014_08005"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOT57070.1"
                     /translation="MPEFVPEVTRVEVAGRRLRLTSLDKVMYPSTETTKGEVLSYYAQ
                     VAPLMLPHLARRPVTRVRYPHGAQDQMFFFEKNLPSGAPSWLPRMPVDDVVYPLIGDL
                     ADLTYLVNLNSLELHVPQWTLTDEGERGNPDRLVIDLDPGKPAGLHECAQVALLVRER
                     LAALGLELYPVTSGSKGMQLYAALGGDLTSDQVRDLVMQLAQELTKKHPDLILWKMTK
                     SLRPGKVFLDWSQNVAAKTTISPYSLRGRELPCVAAPRTWDEVERGAVEAGSLRQLMF
                     DEVLERVARDADLLADLP"
     gene            complement(4354..4956)
                     /locus_tag="PL014_08010"
     CDS             complement(4354..4956)
                     /locus_tag="PL014_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOT57071.1"
                     /translation="MSRRHAWLTFTAGGPPLAHTLMTDAVAWQRALRSLQSRWYATDA
                     PPQVAAAFVLQWLLQVPAHTGAHAAASGPWRADLTHLSFALDPATVPREVRLNGLVAD
                     EGSLEQRFNRAERDYRAVAQPLATAYESQVRLGPHTRSMMVDDMWNAARREAESAAGL
                     LRPGATPRASCCLLYALPGCVECAGCPRLRPSATSGTLVP"
     gene            complement(5013..5348)
                     /locus_tag="PL014_08015"
     CDS             complement(5013..5348)
                     /locus_tag="PL014_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HOT57072.1"
                     /translation="MAKKVQVLLVDDLDKSSPADETVSFSLDGVSYEIDLTSDNAAKL
                     RDDLAVWIGHAERTGGRRSAGRSTAGKGSGRKDVGAIRAWARENGHTVNDRGRISAEI
                     QAAYDKAHA"
     gene            complement(5484..5732)
                     /locus_tag="PL014_08020"
     CDS             complement(5484..5732)
                     /locus_tag="PL014_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57073.1"
                     /translation="MSSIWPYLAAIVPTIVVATMFYFLIKSMLEGDRRERLAQNRFEA
                     ELDRAGRSDANSPSDSVPPPKAPGTTPGTPSTSGDDTP"
     gene            complement(5734..7185)
                     /gene="lysS"
                     /locus_tag="PL014_08025"
     CDS             complement(5734..7185)
                     /gene="lysS"
                     /locus_tag="PL014_08025"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT57074.1"
                     /translation="MRVRRDKRDRLLAAGKHAHPVAVPRTHSLAEVREQWGHLEQGEE
                     TQDVVGIAGRVVFIRNTGKLAFATLQEGVGTRLQVMLSLAEVGEEALADWKAHVDLGD
                     HVFVEGRVISSRRGELSVMASRWEMASKALRPLPVLHKELSEESRVRQRYADLVVRQE
                     ARDMVRTKAAALRAIRGVLDEQGYVEVETPVLQLIHGGAAARPFRTHLNAFDQEMVLR
                     IALELNLKKAVVGGVDRVYEMGRIFRNEGVDATHSPEFTMLEVYQAWGDQTSIGALMR
                     DLYLGVADAVGSRQIETPAGTIDLDGEWRWLPVYDAVSEVVGETVTIDTPKETLLGYA
                     AAHGVEVDPGLTKDKVFLELVGELVEPHLLQPTFLCDYPAIAQPLARPHREKEGLIEA
                     WDLIIAGVERGTGFSELIDPVIQREVLTNQSLLAAGGDPEAMQIDEDFLRALEYGAPP
                     MGGLGLGVDRLIMLFTGSNIRETILFPHLKPEA"
     gene            7329..8087
                     /locus_tag="PL014_08030"
     CDS             7329..8087
                     /locus_tag="PL014_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT57075.1"
                     /translation="MPSARRPWTEGLPARRLERARDAELPASDVWPLTIPAVAQVLAH
                     GIDLAACTVLVGENGSGKSTLVEALAMAAGMNAEGGSTGATHRTHASESPLHEWVTVV
                     RDPGAPRWGYFVRAETMHGLFTWLDTNPGPTDPAFHAMSHGESFVTLLGTRRFRGEGL
                     FVLDEPEAGLSFAAQLHLVAELAEMARRPRTQVVLATHSPVLAAIPGASVLELGEHGI
                     RSTTWDELDVVTHHRSFLAEPRRYLRHVIDDLDD"
     gene            8222..>9537
                     /locus_tag="PL014_08035"
     CDS             8222..>9537
                     /locus_tag="PL014_08035"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOT57076.1"
                     /translation="MSTTPTRITHLIGGTSWQGSPARTSPVFNPATGEQTGVLDLASS
                     ELVGEVVTSAKAAWESSWQRASLTQRTQVLFRFRELLNERKAEIAELITAEHGKVVSD
                     AIGEVTRGLEVAEFACGIPHLLKGGFSENVSTKVDVYSIRQSLGVVAVISPFNFPAMV
                     PLWFVPVAIACGNAVVIKPSEKDPSAVNAVAALWKEAGLPDGILNVVHGDKEAVDALL
                     THPDVKAISFVGSTPIAKYVYETGTAHGKRVQALGGAKNHMLVLPDADLDLAADAAVN
                     AGFGSAGERCMAISALLAVEPIADDLVAKITDRMGKLVTGDGTRGCDMGPLVTAQHRD
                     KVTSYLDAGTRAGATLVVDGRDGDVDADGEGYFLKPTLFDHVTKDMSIYQDEIFGPVL
                     SVVRVASYDEGLKLINDNPYGNGTAIFTNDGGAARRFQNEVEVGMVG"
BASE COUNT         1352 a         3344 c         3372 g         1469 t
ORIGIN      
        1 cctcgtggat cagcccgagc aggatgtgct cggtgccgat gtagttgtgg ttgaggccac
       61 gggcctcttc ctgggcgagc acgacgacgc ggcgggcgcg gtcggtgaac cgttcgaaca
      121 tggtgcttct cctgacggtc gagtcccctc gatgctagtc gcgccgtctg gatgtggctc
      181 gacgttaacc agcctgcggt ttcgccgcga ggggagaact gtcaggccca acgcggtaca
      241 cgcccccggg tgttccgcgt gttcgctctc ggcgcacgcg atcagcaccc tctcgcgtca
      301 gcgacgccgt tggcagcggc cacaccagaa caggttgcgc ccggcaacca cgcgggtgcg
      361 caccttcgac ccgcacgcgg gacacggcat cccggcgcgg cggtagaccg ccgacgagcg
      421 ttcggtgagg cgcggcacct caccgcgacc gagcgcggcc tcgacctcga gcacctggtc
      481 ctcgacggtg acgatgcggc cggtcgtgac ccccaacggc atgagtcgct cgaggtcgtc
      541 ccagatgagg tgccacgagg ctcggcgcag catccgcccc tcggtgaacg gtgagagccg
      601 gtgccggaag agcacctcgg agcggtagac gtttccgaca cctgcgagca ccgcctggtc
      661 catgagcagc tcgccgatgg tgcgcgacga gcggctgatc cgggcccagg cggcgtccgg
      721 gtcggagtcg gggcgcaggg gatcggggcc cagacggtcg agcaccgtgt cgacctgctc
      781 gggcgtggcc acctcgcaca gcgtcggccc gcgaaggtcg gcgacgtgct ggtcggtgcg
      841 cagccgcagg cgcacctgac caaccgccgg cacctccgcc gtccactcgt ggctgtcgac
      901 ggtgaaggag ccgatgaggc ccaggtgcac gtgcagccag cggtcgtcgg caaactcgac
      961 gaacaggtgc ttgcccctcg acgtcgcccg cagcagctcg tggccgctca gcacggccgc
     1021 accctcggcg aacttgccct gggggctcgt cacctcgggg gtggtgcccg cgaaggcctc
     1081 gtggaggtcg cgggcgaggg cgaagagcgt gtggccttca ggcatgcggc ctcagctcgc
     1141 gaaggatgcg cagggccctg ggcccgacgc cgtgcaacgc caggaggtcg cggtccgcgg
     1201 tggaggccac ggcatcccac gtcgtccacc ccgcgctcgc cagggcgcgg cgggccgggg
     1261 ccccgatggc gtgcacctcg tggggtggct ccgagggttc cggcgtcgag gtcggctggg
     1321 ggtgctcgcc ggccacggtc agctggcctt cttgggtgcg gccttcttcg ccgccgcctt
     1381 cttggcgggt gccttctcgg ctgcggcctt cttggtggcc gccgacttgc tggcagcctt
     1441 cttcgcggcc ggggccttgg ccggtgccgg ctcgggagcc tcaccccgcg ctgccttggc
     1501 cttctcgacc gagcgcgcca gtgccgccag caggtcgacg acctcgccgc tctcctccgg
     1561 ctcggccgtg ctgacgcgca cctcgccacc ctcgaccttg gcgcgcacga gctccttgac
     1621 ggcgatcgcg tagtcgtcct cgtagtcgtc cgggtcgtag tcgccggcca gctggtcgat
     1681 gagcatcttg gccatcgcca tctcctgctc ggtggcccgc tggtcgtcgg tgacggtcgc
     1741 gaagtcggcg gcgcgcacct cgtcgggcca cagcatcgtc tgcatgacga tgacgccgtc
     1801 acgcacccgc agcaccgcca tcgtcatccg ggtgcggatg gagacggtgc agatggccat
     1861 ccgcttctcg gtctccaggg cgtcgcgcag caggacgtag ggcttggttg ccgccttgtc
     1921 gggctcgagg tagtacgact tgtccagcca catcgggtcg atctggtcgg ccgggacgaa
     1981 cttcgtgacc ccgatctcct tggaggtgcg gctcggcagg tcggcgaagt cctcgtcggt
     2041 gagcacgacc atgtcgccgt cctcggtctc gtagcccttg gcgatgtcgt cgtacgacac
     2101 ctcctcgccg tcgatcgagc aggtgcgctt gtacttgatc cgcccgccgt cctcgcggtg
     2161 cacctgacgg aactgcacgt cgtggttctc ggtcgcggag tacagccgca ccggcacgtt
     2221 gaccaggccg aaggagaccg cgcccttcca gatcgccctc atggtcgcca cctctcccgg
     2281 atgccgtccg cagcgtctcg cggtcagcgc agactcccag cgtatgccca gccgtgagtg
     2341 cggaagtggt cgctgggacc gccacttccg cactcatcgc gaggcaggat ggggcccgtg
     2401 gccaccctct cctcttccca cgagccgtcc tcggcgcgcg cgccgctgcg gccgatgctc
     2461 gcgacggtgg ccgagcgcgt gccgacgggc gctgactggg tgcacgaggt caagtgggac
     2521 gggatgcggg tgctcgtcga cgtgcgtggc ggcgaggtgc gggtgtggtc gcgcaacgag
     2581 cgggacgtca cggcctccta ccccgagttc gccgggctcg gggaggccta cgacgacatg
     2641 ctcctcgacg ccgaggtcgt cgccctcgac ggcggccggc ccagcttcca cgccctgacc
     2701 gagcgcatgc acgtctcgga ccgtcgcaag gcggtgcggc tggcggccac ccgcccggtg
     2761 accctcatgg tcttcgacct gctgcggctg ttcggggccg acctcaccgg ccagccgtgg
     2821 tcggcgcggc gggaactgct cgaacggctg gacctcaccg gccccacctg gcaggtgcct
     2881 gcggtgcacg acgacggtga gcacctgctg gccgccacgg ccgagcaggg cctcgagggc
     2941 atcgtcagca agcgccgctc ggccacctac gccgccgggc gccgctcgcc cgactggcgc
     3001 aagagcgccc acaaggtcac ccactcggtc gtcgtcggtg gttggcgccc ggaggtcggg
     3061 tcggcgggac ggctcggggc cgtcctcgtg ggcctgcccg acggttccgg tggctggcgg
     3121 tacgccggcc gggtcggctc gggcttggcc ggaacggccg gggaggcgct cgcagtgcgg
     3181 ctgcgcccgc tgagccgcga cgactcgccc ttcgtcgacg acgtgccctc gatcgacgcc
     3241 gtcggcacga cgtgggtgca gccccgggtc gtcatcgagg tgcgtgcgct ggaactgacg
     3301 gggcaacacc gactgcggca accggcatac ctcggggtgc gcaccgacct cgtgcccgcc
     3361 gacctcgagg aggtcgagga tgcctgagtt cgtccccgag gtcactcggg tcgaggtggc
     3421 tggtcgccgg ctccgactca ccagcctcga caaggtcatg tacccctcga ccgagacgac
     3481 caagggggag gtgctcagct actacgcgca ggtggcgccg ttgatgctgc cccaccttgc
     3541 gcggcgtccg gtgacgaggg tgcgctaccc gcacggcgcc caggaccaga tgttcttctt
     3601 cgagaagaac ctcccctcgg gcgcgccgag ctggctgccc cggatgccgg tcgacgacgt
     3661 cgtctacccc ctgatcggcg acctcgcaga cctcacgtac ctggtcaacc tcaactcgct
     3721 cgagctgcac gtgccgcagt ggaccctcac cgacgagggt gagcgcggca atcccgaccg
     3781 cctcgtcatc gacctcgatc cgggcaagcc tgccgggctg cacgagtgcg cccaggtggc
     3841 cctgctcgtg cgcgagcggc tggccgcgct cgggctcgag ctgtacccgg tcacctcggg
     3901 cagcaagggg atgcagctct acgcggccct cgggggtgac ctcacgagcg accaggtgcg
     3961 tgacctcgtc atgcagctcg cgcaggagct gacgaagaag caccccgacc tcatcctgtg
     4021 gaagatgacc aagtcgctgc gccccggcaa ggtcttcctc gactggagcc agaacgtcgc
     4081 cgccaagacg accatcagcc cctactcgtt gcgggggcgg gaactgccct gcgtcgcggc
     4141 gccgcgcacg tgggacgagg tcgaacgcgg tgcggtcgag gcggggtcgc tgcggcagct
     4201 gatgttcgac gaggtgctcg agcgcgtcgc ccgcgacgca gacctgctcg ccgacctccc
     4261 ctgattcctt gcgtccggca ccttggtcgt ccagaccacc aacatgccgg acgtcccgtc
     4321 ggggctgctc cttgcgtccg gcaggttggc tgctcagggc accagggtgc cggacgttgc
     4381 ggaggggcgc aggcgggggc acccggcgca ctcgacacag cccggcaggg cgtagagcag
     4441 gcagcacgag gcccggggcg tggcgccggg gcgcagcaac cccgcggccg actcggcctc
     4501 gcgccgggcg gcgttccaca tgtcgtcgac catcatcgac cgggtgtgtg ggccgaggcg
     4561 cacctgcgac tcgtatgccg tggcgagcgg ctgcgcgacc gcgcggtagt cgcgctcggc
     4621 ccggttgaac cgctgttcga gcgacccctc gtcggcgacg aggccgttca gccgcacctc
     4681 tcgcggcacg gtggcgggat cgagcgcgaa ggacaggtgc gtcaggtcgg cacgccacgg
     4741 gccgctcgcg gcggcatggg cacccgtgtg ggccggaacc tggagcagcc actggaggac
     4801 gaacgccgca gcgacctgtg gcggagcgtc ggtggcgtac cagcggctct ggagcgagcg
     4861 cagcgcgcgt tgccacgcca cggcatccgt catgagggtg tgggcgagcg gcggcccgcc
     4921 cgcggtgaac gtcagccacg cgtggcggcg tgacatgcgc gcagcggcac ccgtcgggtc
     4981 gacggatgcc gctgcggtgc tcgggtcgag gctcaggcgt gcgccttgtc gtaggcggcc
     5041 tggatctcgg cggagatgcg accgcggtcg ttgaccgtgt ggccgttctc gcgggcccac
     5101 gcccggatgg cgccgacgtc cttgcggccg gagcccttgc cagcggtgga gcgaccggcc
     5161 gagcgacggc cacccgtgcg ttcggcgtga ccgatccaca cggcgaggtc gtcgcgcagc
     5221 tttgcggcgt tgtccgaggt gaggtcgatc tcgtagctga caccgtcgag tgagaacgag
     5281 acggtctcgt cagcagggga gctcttgtcg aggtcgtcga cgaggagcac ctggaccttc
     5341 ttagccatgg ttttcccttc aggtcgttgc ggatatcatc cgcggcacgt agcaacatgc
     5401 cgcggacaat ttagcagcgc attcccagaa gtgggaattg ccctgtggac aacattcctc
     5461 ggacaattga atgcgtccag gaatcagggg gtgtcgtcgc ccgacgtcga cggggtgcca
     5521 ggcgtcgttc ccggggcctt gggtgggggt acggaatccg aaggtgaatt cgcatcgctc
     5581 cgccccgcac ggtccagttc ggcctcgaag cggttctgcg cgaggcgctc ccgacgatcg
     5641 ccctcaagca tgctcttgat caggaagtag aacatcgtgg cgacgacaat cgtcggcacg
     5701 attgcggcga gatagggcca gatcgaggac atgtcaggcc tccggcttga ggtgcggaaa
     5761 gagaatggtc tcgcgaatat tggacccggt gaagagcatg atgaggcggt cgacgcccag
     5821 gcccaggcca cccatgggcg gtgccccgta ctcgagcgcg cgcaggaagt cctcgtcgat
     5881 ctgcatcgcc tcggggtcgc ccccggcggc gagcagcgac tggttggtca gcacctcgcg
     5941 ctggatgacg ggatcgatga gctcggagaa gccggtgccg cgctcgacgc cggcgatgat
     6001 gaggtcccag gcctcgatca ggccctcctt ctcgcggtgc gggcgagcca ggggctgggc
     6061 gatcgccggg tagtcgcaca ggaaggtcgg ctggagcagg tggggctcga cgagctcgcc
     6121 gaccagctcg aggaacacct tgtccttggt cagccccggg tcgacctcga ccccgtgggc
     6181 cgcggcgtac ccgagcaggg tctccttggg ggtgtcgatc gtgacggtct ccccgacgac
     6241 ctcgctgacg gcgtcgtaga ccggcagcca gcgccactca ccgtcgaggt caatcgtgcc
     6301 ggcgggcgtc tcgatctgcc gtgatcccac ggcgtcggcg acgccgaggt agaggtcgcg
     6361 catgagggcg ccgatcgagg tctggtcgcc ccacgcctgg tagacctcga gcatcgtgaa
     6421 ctcgggcgag tgggtggcgt cgacgccttc gttgcggaag atgcgcccca tctcgtagac
     6481 ccggtcgacc ccgccgacca cggccttctt gaggttcagc tccagggcga tgcgcagcac
     6541 catctcctgg tcgaaggcgt tgaggtgggt gcggaagggg cgagcggccg cgcccccgtg
     6601 gatcagctgg agcacgggcg tctcgacctc gacgtagccc tgctcgtcga gcaccccgcg
     6661 gatcgcccgc agggccgcag ccttggtgcg caccatgtcg cgggcctcct ggcgcacgac
     6721 gaggtcggcg tagcgctggc gaacccggct ctcctcggac agctccttgt gcagcaccgg
     6781 aagcgggcga agggccttgc tcgccatctc ccagcggctg gccatcaccg acagctcacc
     6841 gcgccgggag ctgatgaccc gaccctcgac gaacacgtgg tcgccgaggt cgacgtgcgc
     6901 cttccagtcg gccagggcct cttcgccgac ctcggccagg ctcagcatga cctgcaggcg
     6961 cgtgccgacc ccctcctgga gcgtcgcgaa agcgagcttg ccggtgttgc ggatgaagac
     7021 cacgcggccg gcgatgccga cgacgtcctg ggtctcctcg ccctgctcga ggtggcccca
     7081 ctgctcgcgg acctcggcca gcgagtgcgt gcgcggcacg gcgacggggt gcgcgtgctt
     7141 tccggcggcg agcagacggt cgcgcttgtc gcggcggacc cgcatctgct cggggaggtc
     7201 gatctcgggg agggcggggg tcgggctctc ggtcacgatc cacaagccta cgggccgccc
     7261 tcggtttccc caccttccct cccacgggtg cggtccgtgg tgtgggtggt cggggctagc
     7321 gtggccggat gccgtccgct cgccgcccgt ggaccgaagg gcttccagcg cgccgcctcg
     7381 agcgagcgcg cgatgccgag ctgccggcat ccgacgtctg gcccctgacg atccccgcgg
     7441 tggcgcaggt cctggcccac ggcatcgacc tggccgcctg cacggtgctc gtcggggaga
     7501 acggctcggg caagtcgaca ctcgtcgagg cgctcgcgat ggcggcgggc atgaacgccg
     7561 agggcggctc gaccggggcc acccaccgca cccacgccag cgagtcgccg ctgcacgagt
     7621 gggtcaccgt cgtacgcgac ccgggggcgc cgcggtgggg ctacttcgtg cgggccgaga
     7681 cgatgcacgg gctgttcacc tggctcgaca ccaaccccgg accgaccgac cccgccttcc
     7741 acgcgatgtc gcacggggag tcgttcgtga cgctgctcgg cacccggcgc tttcgtggcg
     7801 aggggctgtt cgtgctcgac gaaccggagg ccgggctgtc gttcgcggcg cagctgcacc
     7861 tcgtcgccga gctcgccgag atggcccggc ggccgcgcac ccaggtcgtg ctggccaccc
     7921 actccccggt gctggccgcc atcccggggg cgagcgtgct ggaactgggg gagcacggca
     7981 tccggtcgac gacgtgggac gagctcgacg tcgtgacgca ccaccgcagc ttcctcgccg
     8041 agccgcgtcg gtacctgcgg cacgtcatcg acgacctcga cgactgacgg gccacatcga
     8101 gggctgccga cccgagctgc tgatcccaca gggacgaccg gcatcctccg gtgcattccg
     8161 tcagcgggtg cggctctgac acactgaatt cactccactg gcatgacaag gaccgataac
     8221 cgtgagcacg acccccaccc gcatcaccca cctcatcggc ggcacttcct ggcagggcag
     8281 ccccgcgcgc accagcccgg tgttcaaccc cgccaccggc gagcagaccg gcgtgctcga
     8341 cctcgcctcc tccgagctcg tgggcgaggt cgtcacctcc gcgaaggccg cgtgggagtc
     8401 gtcgtggcag cgggcctcgc tgacccagcg cacccaggtg ctcttccgtt tccgggaact
     8461 gctcaacgag cgcaaggccg agatcgccga gctcatcacg gccgagcacg gcaaggtcgt
     8521 ctccgacgcg atcggtgagg tcacccgagg gctcgaggtc gccgagttcg cctgcggcat
     8581 cccgcacctg ctcaagggcg gcttctccga gaacgtctcg accaaggtcg acgtctactc
     8641 gatccgtcag agcctgggtg tcgtcgccgt catcagcccg ttcaacttcc cggcgatggt
     8701 gccgctgtgg ttcgtccccg tggcgatcgc ctgcgggaac gccgtcgtca tcaagccgag
     8761 cgagaaggac ccgtcggcgg tcaacgcggt ggcggcgctg tggaaggaag ccggtctgcc
     8821 cgacggcatc ctcaacgtcg tgcacggcga caaggaagcc gtcgacgcgc tgttgaccca
     8881 ccccgacgtc aaggccatct cgttcgtcgg gtccaccccg atcgcgaagt acgtctacga
     8941 gaccggcacg gcgcacggca agcgtgtcca ggccctcggc ggggcgaaga accacatgct
     9001 cgtgctgccc gacgccgacc tcgacctcgc ggccgacgcc gccgtcaacg ccggcttcgg
     9061 ctcggccggt gagcgctgca tggcgatctc ggccctgctc gccgtcgagc ccatcgctga
     9121 cgacctcgtc gccaagatca ccgaccgcat gggcaagctc gtcaccggtg acggcacccg
     9181 cggctgcgac atgggcccgc tggtcacggc gcagcaccgc gacaaggtga cgtcctacct
     9241 cgacgccggc acgcgggctg gtgccaccct ggtcgtcgac ggtcgcgacg gtgacgtcga
     9301 cgccgatggt gagggctact tcctcaagcc gaccctgttc gaccacgtca ccaaggacat
     9361 gtcgatctac caggacgaga tcttcggccc ggtgctgtcc gtggtgcgcg tcgcgagcta
     9421 cgacgagggg ttgaagctca tcaacgacaa cccgtacggc aacggcacgg cgatcttcac
     9481 caacgacggt ggcgccgcgc ggcgcttcca gaacgaggtg gaggtcggca tggtcgg
//
LOCUS       DAOSOC010000090         9461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7208_length_9461_cov_8.367106, whole genome shotgun sequence.
ACCESSION   DAOSOC010000090 DAOSOC010000000
VERSION     DAOSOC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9461
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7208_length_9461_cov_8.367106"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PL014_08040"
     CDS             <1..753
                     /locus_tag="PL014_08040"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HOT57077.1"
                     /translation="LDHARRTADVGVVLLTGNGPGPDGTGSAGKWSFCTGGDQRIRGR
                     SGYQYADGTTADTVDERRVKAEGGRLHILEVQRLIRTMPKVVIALVGGWAAGGGHSLH
                     VVCDLTIASREHARFKQTDADVGSFDGGYGSAYLAKMVGQKFAREIFFLGRTYSADDM
                     HRMGAVNIVADHAELEAEALEVAREIMGKSPQAQRMLKFAFNLLDDGLMGQQVFAGEA
                     TRLAYMTDEAVEGRDQFLEKRDPDWSPFPWYF"
     gene            complement(813..1472)
                     /locus_tag="PL014_08045"
     CDS             complement(813..1472)
                     /locus_tag="PL014_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57078.1"
                     /translation="MAGRPPLRPTAGTGDADDLARLDAAARHPSMLARVVAGVFTPAN
                     VVLAVMAYVALRHSGSAVEAFGWWALTLVLVVGVPYGILFRALRAGRVDDRQVVRRSQ
                     RPWLFVMALVCVAVALGVLAVAGAPREVVVLVVAMAAGLVAMLVVTLVTKASMHLAVA
                     SGAVAVVLVEQPGVGAVLALLLVPIGWARWRDGRHSVGQLVLGAVIGGVASWVVYGVL
                     R"
     gene            complement(1451..2326)
                     /locus_tag="PL014_08050"
     CDS             complement(1451..2326)
                     /locus_tag="PL014_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT57079.1"
                     /translation="MTFSLGTFASPVDGTALATYTWDAVQAPVGVVQVAHGLAEHAGR
                     YDRLAQALTAAGFVVHATDHRGHGKSISGVPGDFGDAGFEALVADVAAHGASLAAAHP
                     GLPLFLVAHSMGSFAAQSVLLTDSDQYCGVVLSGSTALAELGAAMAAGAQDPDAPAGL
                     EAFNVGYEQRTGYEWLSRDEAEVDAYVADPLCGFDLPEATVPALFGAAGTLADPSALA
                     AIRPGLPVLIASGVDDPLAGGGQLVELLGQRYRDAGLTDVTVRLYEGARHEIFNETNR
                     GEVTDDVIAWLDAHR"
     gene            complement(2382..2831)
                     /locus_tag="PL014_08055"
     CDS             complement(2382..2831)
                     /locus_tag="PL014_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT57080.1"
                     /translation="MSDPNVGVLMFVAHRAMEERVLAALRLAGFDDVTLAQGRVFARI
                     GHGGTRLTDLAEMAQVTKQTAGFLVDRLADAGYVERVPDPSDARARLVRIADRGRVAQ
                     ECAARAVAEVEAEWQAHLGARDMDRLRSIMGRLRVVTDPYRDLDPSQ"
     gene            2971..3624
                     /locus_tag="PL014_08060"
     CDS             2971..3624
                     /locus_tag="PL014_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT57081.1"
                     /translation="MTTISKDESWQVIDAVRTEVADLIDTLTPDQRLQQSLCGAWTIR
                     DVGAHLSMAALASTTFALKNAVRQGFRFDAIIRESTLAWSRGLSDAEVSGRLRTIVGN
                     RTLAPTTMWRDPLLDALVHAEDIARPLGKRVTPHPEAAATATDWAWERTFGFPFFPAR
                     RLRGLRLVATDTDWARGSGQEVHGPVTSLLLLSTGRRTSYDDLTGPGADSARALAGR"
     gene            3701..4432
                     /locus_tag="PL014_08065"
     CDS             3701..4432
                     /locus_tag="PL014_08065"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HOT57082.1"
                     /translation="MSRRRSPGWVPNQHGAWAMLATPLAVGILASSPQWVHLPLTAFW
                     FLGYFAFFAAGLWLKAPPRRRVALVGPLRTYAAASAAMGALTLALDPTLLRWAPLFVA
                     PLAVGLVASARRDERSLWSGLATSIGSCLMVLAAYDAGGGTDWVRAWLLTGILAAYFA
                     GTVLYVKTMIRERGDERFHWLSALAHGSATVAMTWVSPWLVLVFALLTIRAAVLPAFR
                     GSPKAVGIGEIIATVAVAVTALLVT"
     gene            complement(4461..4916)
                     /locus_tag="PL014_08070"
     CDS             complement(4461..4916)
                     /locus_tag="PL014_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57083.1"
                     /translation="MDEAYAAYLRAVQEQRAELGESMVALQGALDLPAGLGPTWRGRV
                     RAALTELAHDLRDHVELTEAAGGFYDDIRTAAPRLNHQVEEQLAEHAALLAEVERLLA
                     ERDDGVRGADAVVAHREAATRLLEQLVRHRQRGSDLTFEAYAVDLGGSG"
     gene            4990..6183
                     /gene="menE"
                     /locus_tag="PL014_08075"
     CDS             4990..6183
                     /gene="menE"
                     /locus_tag="PL014_08075"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HOT57084.1"
                     /translation="MTGHHHDGAPAVEALAVPAGPTILDLLPRLAEALSGDRPVMPHA
                     ASGPVPPVPGHDPASLPDGLAVVVGTSGSTGTPKRAMLTAPALRASARATHATLGGPG
                     QWLLAIPAHHIAGLQVLVRSLDAGTPPLAMDLTDGFRPAAFIAATQGFIAGARRYTSL
                     VPTQVVRLLDHPGGGDALRTFDAVLVGGAAMPPRLRARAERARVHLVATYGMSETAGG
                     CVYDGRALPRTEVELDEEARILLGGPTIAHGYLGLPALTRETFGTDEDGIRWFRTDDV
                     GHVDDDGTLHVDGRVDDLIITGGLKVAPRLVEEAILEHVPGIREVVVVGSPDHEWGAC
                     VSALVVLSPDAVHDVTAADLRAHLRGILPDHALPRRALTSPSIPIRGPGKPDRRAVEA
                     LFTVG"
     gene            6191..6445
                     /locus_tag="PL014_08080"
     CDS             6191..6445
                     /locus_tag="PL014_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HOT57085.1"
                     /translation="MLRYLPVILSLALTVYCVVDAIQTQDGAARNLPKIVWLLLILLF
                     PIVGPAAWLIAGRPRKGPRGLPQQRRPRGPDDDPDFLKDL"
     gene            6454..7341
                     /locus_tag="PL014_08085"
     CDS             6454..7341
                     /locus_tag="PL014_08085"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HOT57086.1"
                     /translation="MASLNQWVAGARPRTLPAAIAPVILGTAAAHLLGRGDLALAILA
                     LLVALSLQVGVNYANDYSDGIRGTDEARVGPVRLVGQRLAAPANVKAAAMLSFGAAAM
                     AGLALVGLSEAWIMLPLGALSVLAAWRYTGGDNPYGYRGLGEVYVFVFFGLMATLGTL
                     YTQADAVSWFGLIGAIGVGALASAILVANNLRDIPTDIEHGKRTLAVRLGDARTRALY
                     VALFAASVVAVLGMAWFEPWALLGLLAAPLGWTAMRTVRAGTTGRELIPVLAATGLYE
                     VAYAVLIALGVVIGVAIAT"
     gene            complement(7372..7674)
                     /locus_tag="PL014_08090"
     CDS             complement(7372..7674)
                     /locus_tag="PL014_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HOT57087.1"
                     /translation="MLRYTVLRLLVFLGVLSALWLLGLRGQENMLLLVAGAALLSAVI
                     SYFGLRRFREEYSAQLAQKIEQRIETKQAKQSAAGAGATDEDAEDAEVARGDDTFR"
     gene            complement(7681..8724)
                     /gene="ccsB"
                     /locus_tag="PL014_08095"
     CDS             complement(7681..8724)
                     /gene="ccsB"
                     /locus_tag="PL014_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOT57088.1"
                     /translation="MTDQDLAQLSNTAIYSAMVVLTLAMLAYAVYLARLAPTRGDETG
                     TDAVEGLDGARELVGADGRAVASDAAPRDGAPTAGGGRTASGTTSDRGRESEPLAARK
                     AAGIGWMLTLLATVLVVAGVVLRALEVHRWPLGNMYEFAIVGAMFVLVSYCAWAFRRD
                     LRWLGLFVVTPVLLNLGLAITVWYTDASELMPSLRSVWLAIHVAVATFSVAVFTIAFS
                     LGILYLVQDRLESTPGRKRSFMDRLPDARSLERLTYAVHIVAFPLWTFTVIAGAIWAR
                     QAWGSYWNWDPKEVWSFVIWVVYAAYLHARATTGWKRQNAVWIALAGYGCIIINFAVV
                     NVFFVGQHSYSGL"
     gene            complement(8721..>9461)
                     /locus_tag="PL014_08100"
     CDS             complement(8721..>9461)
                     /locus_tag="PL014_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOT57089.1"
                     /translation="QGTSEQRSIRVNHPLETGGGTVFLLGNGYAPKITVRDGEGTVIY
                     SDATPFLPQDNFYTSVGAVKVPGATPKQLGFAGFFLPTGIIDDQGPRSLFPDALDPQL
                     ALTAFEGELFPNGRPQSVYSLDTEAMKAIPAADGNDQLRILLRPGQTFELPEGRGSIT
                     FDEVERFAGLSIRTDPGKSLTLVAALASLAGLVASLVIRRRRVFVRVGPGEGHSRTLV
                     AVGGLAKDDDDGMPAEIEHLLTALEGRR"
BASE COUNT         1402 a         3594 c         3179 g         1286 t
ORIGIN      
        1 ctcgaccacg cacgccgcac ggctgacgtc ggcgtcgtcc tgctgaccgg caacgggccg
       61 ggccccgatg gcaccggcag cgcgggcaag tggtcgttct gcaccggcgg cgaccagcgc
      121 atccgcggcc gctcgggcta ccagtacgcc gacggcacca ccgccgacac cgtcgacgag
      181 cgccgggtca aggccgaggg cgggcgcctg cacatcctcg aggtgcagcg cctgatccgc
      241 accatgccca aggtcgtcat cgccctcgtg ggcggatggg cggccggcgg gggccacagc
      301 ctgcacgtcg tgtgcgacct caccatcgcc tcgcgtgaac acgcccgttt caagcagacg
      361 gatgccgacg tcggcagttt cgatggcggc tacggcagcg cgtacctcgc caagatggtg
      421 ggccagaagt tcgcccgcga gatcttcttc ctcggccgca cgtactccgc cgacgacatg
      481 caccgcatgg gcgccgtgaa catcgtcgcc gaccacgccg agctcgaggc cgaggcactt
      541 gaggtcgccc gggagatcat gggcaagagc ccgcaggcgc agcgcatgct gaagttcgcg
      601 ttcaacctgc tcgacgacgg gctcatgggc cagcaggtct tcgccggcga ggcgacccgc
      661 ctggcctaca tgaccgacga ggccgtcgag gggcgcgacc agttcctcga gaagcgcgac
      721 cccgactggt cccccttccc ctggtacttc taggcacgtc ctgccactcg acgaggcgag
      781 ccatcccacg gccctgccgt gaccacgggt cgtcagcgca acaccccata gaccacccac
      841 gacgccaccc cgccgatgac ggcaccgagc acgagctgcc ccaccgagtg ccgcccatcc
      901 cgccagcgcg cccacccgat cggcacgagc agcagcgcca gcacagcacc gaccccgggc
      961 tgctcgacta gcaccacggc caccgccccc gacgccacgg ccaggtgcat cgacgccttg
     1021 gtcaccagcg tcacgaccaa catcgccacc agtccggccg ccatcgccac gacgagcacg
     1081 acgacctccc gcggcgcacc ggccacggcc aacacaccca gagcgaccgc cacacacacc
     1141 agcgccatga cgaacagcca cggccgctgc gagcgccgca ccacctgccg gtcgtccacc
     1201 cgcccggccc gcagcgcgcg gaacaggatg ccgtagggca cgcccacgac gagaacgagc
     1261 gtcagcgccc accacccgaa tgcctcgacc gcgctgcccg agtgccgcag ggcgacgtac
     1321 gccatgaccg cgagcaccac gttcgccggg gtgaacacgc ccgccaccac gcgggcgagc
     1381 atcgacggat gccgtgccgc ggcatccagc ctcgcgaggt cgtccgcgtc ccccgtccct
     1441 gccgtgggcc tcagcggtgg gcgtccagcc acgcgatcac gtcgtcggtg acctcgccgc
     1501 ggttggtctc gttgaagatc tcgtgccgcg caccctcgta caggcgcacc gtcacatccg
     1561 tcaggccggc gtcgcggtag cgctgcccga gcagctccac gagctgaccg ccacccgcga
     1621 gcgggtcgtc gacgccggat gcgatgagca cgggcaggcc cgggcggatc gccgcgagcg
     1681 ccgaggggtc ggcgagcgtc ccggcagccc cgaagagcgc cgggaccgtc gcctccggaa
     1741 ggtcgaaacc acacagcgga tccgcgacgt acgcgtccac ctcggcctcg tcgcgcgata
     1801 gccattcgta gccggtgcgc tgctcgtagc cgacgttgaa cgcctcgagg ccagccgggg
     1861 catcggggtc ctgcgcaccg gccgccatgg cggcgcccag ctcggcgaga gccgtcgagc
     1921 ccgagagcac gaccccgcag tactggtcgg aatcggtgag gagcaccgac tgggcagcga
     1981 acgaccccat cgagtgcgcg acgaggaaca gcggcagccc ggggtgcgct gcggccaggc
     2041 tggcgccgtg ggcggcgacg tcagcgacga gcgcctcgaa cccggcatcc ccgaagtcac
     2101 ccggcacccc ggagatcgac ttcccgtggc cacggtggtc ggtggcgtgg acgacgaagc
     2161 ccgccgcggt gagcgcctgc gcgaggcggt cgtagcgacc tgcgtgctcg gcgagcccgt
     2221 gggccacctg gacgaccccg acgggcgcct gcacggcatc ccaggtgtag gtggcgagtg
     2281 cggtgccgtc gacgggggac gcgaacgtgc cgagggagaa ggtcatgccg gcgattgtgg
     2341 cagggccggc cgcagcacgg cgaggacacg acggcccacg atcactgcga ggggtcgagg
     2401 tcccggtagg ggtcggtcac gactcgcagt cgccccatga tcgagcgcag ccggtccatg
     2461 tcgcgtgctc cgaggtgtgc ctgccactcg gcctcgacct ccgcgaccgc ccgggctgcg
     2521 cactcctggg ccacccggcc gcggtcggcg atccgaacga gcctggcccg cgcatcgctg
     2581 gggtccggca cccgctcgac gtacccggca tccgcgagcc ggtcgacgag gaagccggcc
     2641 gtctgcttgg tgacctgggc catctccgcg aggtcggtca ggcgggtccc cccgtgcccg
     2701 atcctcgcga acaccctccc ctgcgcgagg gtcacgtcgt cgaatccggc gaggcgcagg
     2761 gcggcgagca cgcgctcctc catcgcgcga tgggccacga acatgagcac cccgacattg
     2821 gggtccgaca aaacccgtca cctccgacgt gatgcgatcg ataccgggcg gtaggacgac
     2881 aaccgaggat gccgtgcctt gccatccgaa tgttcgtcag actatcgtac caatatggtt
     2941 cgatagtctg acggtatcgg agacgagcgc atgaccacca tcagcaagga cgagagctgg
     3001 caggtgatcg acgcagtgcg caccgaggtc gccgacctga tcgacaccct cacccccgac
     3061 caacgcctgc agcagtcact gtgcggcgcc tggaccattc gtgacgtggg cgcccacctg
     3121 tcgatggccg cgctcgcgtc gaccacgttc gccctcaaga acgctgtgcg gcagggtttt
     3181 cgattcgacg cgatcatccg cgaaagtacc ctggcctggt ctcgcggcct gtcggatgcc
     3241 gaggtgagtg gcaggctgcg cacgatcgtc ggcaaccgca cgctggcacc gacgacgatg
     3301 tggcgcgacc ccctgctcga tgccttggtg cacgcagagg acatcgctcg cccgctcgga
     3361 aagagagtga cccctcaccc cgaggcagcg gcaaccgcca ccgactgggc ctgggagcgc
     3421 accttcggat tcccgttctt ccccgcacgc cggttgcgcg gtctgcgcct cgtcgccacg
     3481 gacaccgact gggcccgcgg ttcagggcaa gaggtccacg gaccagtcac ctccctgctg
     3541 ctcctgtcga ccgggcgccg gacgagctac gacgacctca ccggccccgg cgccgacagc
     3601 gcccgcgccc tggcaggacg gtgacccccg cgtggcctct cgcgtgacct ctcgctgacc
     3661 cggtggccga gcgccgcgct cagcggccta ccctcgacag gtgagccgca gacggtcgcc
     3721 ggggtgggtc cccaaccagc acggggcctg ggcgatgctc gccaccccgc tcgcggtcgg
     3781 catcctcgcc agcagcccgc agtgggtcca cctgcccctg accgccttct ggttcctggg
     3841 ctacttcgcg ttcttcgccg ccgggctgtg gctcaaggcg ccgccccgtc ggcgcgtggc
     3901 actcgtgggt cccctgcgca cgtatgccgc ggcgtccgcg gcgatgggag ccctcaccct
     3961 cgcgctcgac cccacgctgc tgcggtgggc cccgctgttc gtcgcaccgc tggccgtcgg
     4021 tctcgtggcc agcgcccgac gtgacgagcg cagcctgtgg agtggactcg cgacgagcat
     4081 cggctcctgc ctcatggtgc tcgccgcgta cgacgccggc ggcggcaccg actgggtgcg
     4141 ggcgtggctg ctgaccggca tcctcgccgc gtacttcgcc ggcacggtgc tctacgtcaa
     4201 gacgatgatc cgcgaacggg gcgacgaacg cttccactgg ctctcggccc tggcgcacgg
     4261 ctcggcgacc gtcgcgatga cgtgggtgag cccgtggctg gtgctcgtgt tcgccctgct
     4321 gacgatccgc gcggcggtcc tgcccgcgtt ccggggctca ccgaaggccg tcggcatcgg
     4381 cgagatcatc gcgaccgtcg cggtcgccgt gaccgccctg ctcgtcacct agtccgcact
     4441 ggccgcggcc cggaccggcc tcagccactc ccgccgaggt cgaccgcata ggcctcgaac
     4501 gtcaggtccg acccgcgttg ccggtgccgc acgagttgct cgagcaaccg ggtcgctgcc
     4561 tcccggtggg cgaccacggc gtccgcgcca cgaaccccgt catctcgctc ggcgagcaac
     4621 cgctcgacct cggccagcag ggctgcatgc tcggccagct gctcctcgac ctggtggttc
     4681 aggcgcggag cagccgtgcg gatgtcgtcg tagaaccccc cggcggcctc ggtgagctcg
     4741 acgtggtcgc gcaggtcgtg ggccagctcg gtcagcgcgg cccgcacccg cccccgccac
     4801 gtcggaccga gcccggccgg caggtcgagc gccccttgga gggccaccat cgactcaccg
     4861 agctcggccc gttgctcctg caccgctcgc aggtacgcgg catacgcttc gtccacggtc
     4921 cacctcctgg gcccgatggt ccagacccca tcatccaccg gcggcccacg attggcgagg
     4981 ggagcagcga tgaccggcca ccaccacgac ggcgcacccg ccgtcgaagc actcgcagtg
     5041 ccggccgggc ccaccatcct cgacctgcta ccccggctcg ccgaggccct gtccggcgac
     5101 cggccggtga tgccacacgc cgcctccggc cccgtcccgc ccgtccccgg ccacgacccg
     5161 gcctcgctcc ccgacgggct cgcggtcgtc gtcggcacct cgggctccac gggcaccccc
     5221 aagcgggcga tgctcacggc accggccctg cgcgccagcg cccgcgccac ccacgccacc
     5281 ctcggcggcc ccggccagtg gctgctcgcc atcccggccc accacatcgc cggcctccag
     5341 gtgctggtgc ggtcgctgga tgccgggacg ccgcccctcg cgatggacct caccgacggg
     5401 ttccgccccg cggcgttcat cgcagccacc caggggttca tcgccggtgc ccgccggtac
     5461 accagcctcg tcccgaccca ggtggtccgc ctgctcgacc accccggagg cggcgacgcc
     5521 ctgcgcacgt tcgacgccgt gctcgtcggt ggcgccgcga tgccgccccg gctgcgcgcg
     5581 agagccgagc gggcccgggt gcacctcgtc gcgacctacg gcatgagcga gaccgcgggc
     5641 gggtgcgtct acgacggtcg ggccctgccc cgcaccgagg tcgagctgga cgaggaggct
     5701 cgtatcctgc tcggcggccc gacgatcgcc cacggctacc tcggcctccc cgccctcacg
     5761 cgagagacgt tcggaaccga cgaggacggc atccggtggt tccgtaccga cgacgtcggc
     5821 cacgtcgacg acgacggcac cctgcacgtc gacggccgcg tcgacgacct catcatcacc
     5881 ggtggcctca aggtcgcccc gcggttggtg gaggaggcga tcctcgagca cgtgcccggc
     5941 atccgggagg tcgtcgtcgt cggcagcccc gaccacgagt ggggggcctg cgtgtccgct
     6001 ctcgtcgtgc tctccccgga cgccgtccac gacgtcaccg ccgccgacct gcgagcccac
     6061 ctgcgcggca tcctgcccga ccacgccctg ccacgacggg ccctgacctc accctcgatc
     6121 ccgatccgag gccccggcaa gcccgaccgc cgtgcggtgg aggccctgtt caccgtggga
     6181 tgatggggca gtgctgcgtt acctgcctgt catcctcagc ctcgcgctga cggtctactg
     6241 cgtggtcgac gccatccaga cccaggacgg cgccgcgcgc aacctgccca agatcgtctg
     6301 gctgctcctc atcctgctct tcccgatcgt cggtccggcc gcctggctca tcgccggacg
     6361 cccccgcaag ggtccgcgcg gcctgcctca acagcgccgc ccccgcggcc cggacgacga
     6421 ccccgacttc ctcaaggacc tctgaacacc ctcatggcat ccctgaacca gtgggtggct
     6481 ggtgcccgcc cgcgcaccct gcctgccgcc atcgcccccg tcatcctcgg aaccgctgcc
     6541 gcccacctgc tcgggcgcgg cgacctggcg ctggcgatcc tcgccctcct cgtcgccctc
     6601 tcgctgcagg tcggggtgaa ctacgcgaac gactactccg acggcatccg cggcaccgac
     6661 gaggcccggg tcggcccggt gcgcctcgtg ggccagcgtc tggcggcccc ggcaaacgtc
     6721 aaggcggcgg cgatgctgag cttcggcgcc gcggccatgg caggcctggc cctcgtcggc
     6781 ctcagcgagg cgtggatcat gctgccgctc ggtgccctct cggtgctcgc ggcctggcgc
     6841 tacaccggcg gcgacaaccc ctacggctac cgcggcctcg gtgaggtcta cgtcttcgtc
     6901 ttcttcggtc tgatggccac gctcggcacg ctctacacgc aggcggatgc cgtgtcctgg
     6961 ttcgggctga tcggcgccat cggggtcggt gccctggcgt cggcgatcct cgtcgccaac
     7021 aacctgcgcg acatcccgac cgacatcgag cacggcaagc gcacgctcgc ggtgcgactc
     7081 ggcgacgcgc gaacccgcgc tctctacgtc gcgctgttcg cggcatccgt cgtcgcggtg
     7141 ctcggcatgg cgtggttcga gccctgggcg ctgctcggcc tgctcgccgc gccgctcggg
     7201 tggaccgcca tgcgcaccgt acgggccggc accacggggc gcgagctgat cccggtgctc
     7261 gccgccacgg ggctctacga ggtggcgtat gccgtcctca tcgccctcgg tgtcgtcatc
     7321 ggcgtcgcga tcgccacctg accctcacca cctgctgcga ggtcagcccg gtcagcggaa
     7381 ggtgtcgtcc ccacgcgcga cctcggcatc ctcggcgtcc tcatcggtcg cgccggcccc
     7441 ggcagccgac tgcttggcct gcttggtctc gatgcgctgc tcgatcttct gcgcgagctg
     7501 tgccgagtac tcctcccgga agcgccgcaa cccgaagtac gagatgacgg ccgacagcag
     7561 ggcggcaccg gcgacgagca ggagcatgtt ctcctggccg cgcagcccga gcagccatag
     7621 cgcggacaac acaccgagga agacgagcag gcgaagaacg gtgtaccgaa gcacgtcaga
     7681 tcacaacccg gagtaggagt gctggccgac gaagaacacg ttgaccacgg cgaagttgat
     7741 gatgatgcag ccgtacccgg ccagcgcgat ccacacggcg ttctggcgct tccacccggt
     7801 cgtggcgcgg gcgtgcaggt acgcggcgta gacgacccag atgacgaagc tccagacctc
     7861 cttggggtcc cagttccagt acgaacccca ggcctgccgg gcccagatgg ccccggcgat
     7921 gacggtgaag gtccacagcg ggaacgccac gatgtgcacc gcgtaggtca accgctccag
     7981 cgaacgcgcg tcgggcagcc ggtccatgaa gctgcgcttg cggccggggg tcgactcgag
     8041 ccggtcctgc acgaggtaga ggatgccgag cgagaaggcg atggtgaaca cggccaccga
     8101 gaaggtcgcg accgcgacgt ggatggcgag ccacaccgag cgcagcgacg gcatgagctc
     8161 cgaggcgtcg gtgtaccaga cggtgatggc cagcccgagg ttgagcagca cgggggtgac
     8221 gacgaacagg ccgagccagc gcaggtcacg ccggaacgcc cacgcgcagt acgacaccag
     8281 cacgaacatc gccccgacga tcgcgaactc gtacatgttg cccagcggcc accggtgcac
     8341 ctcgagcgcc cgcagcacca caccggcgac gacgagcacg gtggcgagca gggtgagcat
     8401 ccacccgatg ccggccgcct tgcgggctgc cagtggctcg ctctcgcgcc cacggtccga
     8461 cgtcgtaccg gatgccgtcc gcccacctcc cgctgtcggc gcaccatcac ggggggcggc
     8521 atccgacgcg acggcgcgac cgtcggcccc gacgagctcg cgggccccgt caaggccctc
     8581 gacggcatcc gtgccggtct cgtcaccacg ggtgggggcg aggcgcgcga ggtacacggc
     8641 gtaggcgagc atcgcgaggg tgagcaccac catcgcggag tagatggccg tgttcgacag
     8701 ctgggcgagg tcttggtcgg tcatcgacgt ccttcgagtg cggtcaggag atgctcgatc
     8761 tcggcgggca tcccgtcgtc gtcgtccttg gcgaggccgc cgacggcaac cagcgtacga
     8821 ctgtgtccct cacccggccc aacccgcacg aacacccttc gacggcggat gaccagggag
     8881 gccacgagtc cggcgaggct ggcgagcgcg gcgacgaggg tgaggctctt gccggggtcg
     8941 gtgcggatcg acaggccggc gaagcgctcg acctcgtcga aggtgatcga cccacgaccc
     9001 tccggcagct cgaaggtctg ccccggccgc agcaggatgc gcagctggtc gttgccatcg
     9061 gccgcaggga tggccttcat cgcctcggtg tcgagggagt agaccgactg cggccggccg
     9121 ttcgggaaca gctcgccctc gaaggccgtg agtgccagct gggggtcgag ggcgtcgggg
     9181 aagagcgagc gcgggccctg gtcgtcgatg atccccgtcg ggaggaagaa cccggcgaag
     9241 cccagctgct tcggcgtcgc ccccggcacc ttgacggcac cgacggaggt gtagaagttg
     9301 tcctgcggca ggaacggggt ggcgtcgctg tagatgaccg tgccctcgcc gtcgcgcacg
     9361 gtgatcttcg gggcgtagcc gttgccgagg aggaacacgg tgccgccgcc ggtctccagg
     9421 gggtggttca cccggatcga ccgctgctcg ctcgtgccct g
//
LOCUS       DAOSOC010000091         9368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7296_length_9368_cov_10.247074, whole genome shotgun sequence.
ACCESSION   DAOSOC010000091 DAOSOC010000000
VERSION     DAOSOC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7296_length_9368_cov_10.247074"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1182
                     /locus_tag="PL014_08105"
     CDS             211..1182
                     /locus_tag="PL014_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOT57090.1"
                     /translation="MTEGTVRWFDADRGFGFIALGEDTDDLYVHASEIISNDGVRVLR
                     EGQVVEFEVGEGDRGPQARRVRVTADHAADAPLGVLGTVAWYEPTKGYGFVTPDGSEG
                     QVFVHASAIIGGGVVTEGQRVAFLVVDGEKGPQADPLLPLGAQAAPAAQHAGASGRAE
                     RSDGADGTVTWYDVDKGFGFITPESGGQDVFAHARDLADGLYELAEGDRVTYGVVDSE
                     KGPQARDVRLAGSSTRRSAPPRQASRPRSDQRRSSGAPARGGEGVIVRYDPDRGFGFI
                     APDSGGDDLFVHVSVIRGSDHLYQGDRVRFQVRQSDRGPQADRVELI"
     gene            complement(1187..1705)
                     /locus_tag="PL014_08110"
     CDS             complement(1187..1705)
                     /locus_tag="PL014_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT57091.1"
                     /translation="METLVLTVISDDRPGLVATLSASLAARGATWQRSQLSQLAGKFA
                     GIVEVVVPADGVDGLVADLDGLSAEGITVSTTRTSASSEAPTHHLTLDLVGADRPGIV
                     AEVSALLSARGIGIVELETFVSEAPMAGGMLFEAHAVLAAPAGADEGELRAAMESLAD
                     ELMVDVALAEDA"
     gene            1789..2583
                     /locus_tag="PL014_08115"
     CDS             1789..2583
                     /locus_tag="PL014_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOT57092.1"
                     /translation="MSTPHLIALDIDGTTVNHEGALSPAVRDAVTAVADAGHHVTIAT
                     GRGIIGTLPILDRLGLTTGYAVCANGAITLRLDPTSDAGYEVVESVTFDPAPALTALR
                     EHFPSALVAVEELGVGFKVSAPFPDGELDGIPEVVTFDELMSTPVTRVTLRDPSATSE
                     EFIERTARIGLHGVEYAVGWSAWLDITPEGVSKGAALEVIRRWLAIEPQYTVAVGDQR
                     NDFEMLRWAARGVAMGNAPDEVKAVADEVTGDVDDDGLVDVLTSLL"
     gene            2673..3461
                     /locus_tag="PL014_08120"
     CDS             2673..3461
                     /locus_tag="PL014_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HOT57093.1"
                     /translation="MDSTDVSSAARKAGDHPVVEKGARIGYAMSGVIHFIIGWIALKL
                     AWGIGGGSGDADTSGALKTVASSGGGPLMLGFAVLGFLMLAVALAAAAIVGGETSDRI
                     KSAAKAVMYTSFAWSSFAIARGASNSDEAKTDDVTAQVMGMPGGRYIVGLVGLVVLGV
                     AGYHVYKGWSKKFLEDLEEHPGDWAVTAGRLGYVAKGCALFVVGLLFLLAAWHADSEE
                     AAGLDGALTTIRDLAFGPYLLTLVAAGIASYGVYSFARARYARL"
     gene            complement(3555..4262)
                     /locus_tag="PL014_08125"
     CDS             complement(3555..4262)
                     /locus_tag="PL014_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT57094.1"
                     /translation="MGFLTRWDDDQSRPTLKAALRDLGVRAVLPATALLAVIIGFGYL
                     LKGPLFELSEDEKQINREFQDARDGLWNSITHLMSSIGNTEYVIGVCLIVIGLVWWRT
                     KQWWYAVVPGIAISLQATVFVIATHVVGRARPTVERLDPTPPTSSFPSGHVGASTALY
                     VALGLMATRIDNPLVRRLAVTWCVLAPAFVAYARLYRGAHHVTDILFGILNGVVCAVL
                     AWLYLGRKAAVASRPMT"
     gene            complement(4262..4939)
                     /locus_tag="PL014_08130"
     CDS             complement(4262..4939)
                     /locus_tag="PL014_08130"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT57095.1"
                     /translation="MDDTLTAPAPPTRAQRLVRALTYALPVAAVLFGVGLLVRSEWDP
                     LIRLDDAIIERATAYTRPREGLTDFLVRWQELTQPFKLYLVGTALCLWAWLAKGLRTR
                     AAWAFITMMLAWMVGLGAKYLFQRARPVLEDPVSTSPGYSFPSGHALNSAAWATIVVL
                     LLWPLLRSTASRVVAVTAAVAMVTVTASDRVLLGVHFPSDVTVGVITGVGLVLASYAG
                     YRRQEGQ"
     gene            5034..5840
                     /locus_tag="PL014_08135"
     CDS             5034..5840
                     /locus_tag="PL014_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT57096.1"
                     /translation="MAELHVTSASNPRLKAVLALRRRRVREDEGLTVVDGYEELTLAL
                     DAGVVPRTVLHCPELMLDPAAQGELVQRARDLGASTVRLSRGAFEKVAYREGPDGFLA
                     VVPVAGVALGDLDLPPDPLVLVCEGLEKPGNLGAVLRTADAAGVAAVVAADPVTDWGN
                     PNVVRASKGTVFSVPVASATTEEVVVWLRRNGIRLIATTPDTDTAHTDVDYRGGVAIA
                     VGTEKQGLTDAALTAADARVRIPMVGRVNSLNAATAAAVVVYEAVRQRSS"
     gene            5949..6584
                     /locus_tag="PL014_08140"
     CDS             5949..6584
                     /locus_tag="PL014_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT57097.1"
                     /translation="MRVDHVVYAAERDGLKATAQRLAEQIGVTPVDGGIHPRFGTRNI
                     ILPLAHERYVEVVEVLDHPASDKAPFGQAVRARSEAGGGWLGWVVQVDDITEQESRLG
                     REAVDGNRHRPDGVELRWRQLGIKGLQSDPQLPYFIEWAQGVPHPSGDAATEVTIESL
                     QIAGDPDRVREWLGLPADSTSTVIDFKFVSPHGTPGLMAVTFDTPNGDVTI"
     gene            complement(6606..8972)
                     /locus_tag="PL014_08145"
     CDS             complement(6606..8972)
                     /locus_tag="PL014_08145"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT57098.1"
                     /translation="MTQTTDRPQHDHDEVREVDLAITGMTCASCSARIEKKLGKLDGV
                     EAVVNLATEKATVRYAGAVTVEDLLRTVRATGYGAEVVRGGPQPEAAGSTRTPSDLLV
                     GTGTPGGGSARTPSDLLVDSGTHENHLDHAVAPEDALKRRALVAAVLTVPVVILAMVP
                     GIHLDSSPWVQLALATPVVLWAGWPFHRAAALNARHGASTMDTLVSIGVLTAYLWSAV
                     VVLTGRASTMADMGTTNGVDAAGTTHLWFETATVVTTFLLIGRWMEARATDRTRDALR
                     GLMSLGAKDVAVERVDPTTRATTEVRIPVDQLGAGDRFVVRPGEKVATDGRIVEGSSA
                     VDASLVTGESLPVDVAIGDDLVGGSINTTGRLVVEATRVGADTTLAGIARLVERAQTT
                     KAPIQRLADRVSAIFVPIVLVLAALTFLGWWIGTGDPARALEIAIAVLVIACPCALGL
                     ATPTALLVGTGRGARLGILIKGADVLEDTRRIDTVVLDKTGTVTSGDMTLVDVATAGR
                     LPKDQALRAAAAVETGSEHPVARAVVTAAASRAIDVPRATDFVSLPGAGARAIIKGTE
                     VTVGRADLFDDVPQELARPTVDGTTVWVGWGGRAHASLTVADTVRRSSQRAVRDLAEL
                     GLPAYLLTGDHDQAALTVARDVGIPVGRVIADVRPEDKHAMIADLQKRGKVVAMVGDG
                     VNDAAALAQADLGIAMGTGTDVAADSADIVLLRSELPAVVDAIELSRATLRTIKQNLG
                     WAFGYNIAAIPLAMAGLLNPMIAGGAMAASSVLVVSNSLRLRRWSREG"
     gene            complement(8974..9279)
                     /locus_tag="PL014_08150"
     CDS             complement(8974..9279)
                     /locus_tag="PL014_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOT57099.1"
                     /translation="MTEHTATVAPGYAGHKDDHLKRLRRIEGQVRGIARMVESDAYCI
                     DVLTQVSAVTRALQAVSLGLLEEHIEHCVVDAARESDEAAAVKVAEATAAIARLVRS"
BASE COUNT         1353 a         3292 c         3383 g         1340 t
ORIGIN      
        1 aggaaggttg cccaacgcaa gggattcctt gcctggcgca agggctgctg gcagtacccc
       61 cttgggcccc gtgtctcacg cctcacacgc cgctcctcac aagccccgcc tcgggcgtca
      121 cgcctcgcgc cttgtgcgtg gttccctgcc tgcggctcgt cggcgacaat gatccagcac
      181 accgaccgta gcttcgacga ggagtcagcc gtgaccgagg gaaccgttcg ctggttcgat
      241 gccgaccggg gcttcggctt catcgccctg ggggaggaca ccgacgacct gtacgtccac
      301 gcctccgaga tcatcagcaa cgacggggtg cgcgtgctgc gtgagggcca ggtcgtggag
      361 ttcgaggtcg gcgagggcga ccgcggcccg caggcacgac gtgtccgggt cacggccgac
      421 cacgccgccg atgcaccctt gggtgtgctc ggcacggtcg cctggtacga gccgaccaag
      481 ggctacggct tcgtcacgcc cgacggcagt gagggccagg tgttcgtgca cgcgtcggcc
      541 atcatcggcg gcggcgtcgt gactgagggg cagcgcgtgg cgttcctcgt cgtcgacggg
      601 gagaagggcc cgcaggccga ccctctgctg cctttggggg cgcaggccgc acctgccgcc
      661 cagcacgccg gggcgtcagg ccgggcggag cggtcggacg gcgcggacgg caccgtgacc
      721 tggtacgacg tcgacaaggg cttcgggttc atcaccccgg aatctggtgg ccaggacgtc
      781 ttcgcgcacg cccgggacct cgcggacggg ctctacgagc tggccgaggg cgaccgggtg
      841 acctacggcg tggtcgacag cgagaagggc ccgcaggcgc gcgacgtgcg cctggcaggc
      901 agctcgacgc ggcggtcggc gcccccgagg caggcgtccc ggccccggtc cgaccagcgc
      961 cgctcctccg gcgcaccggc gcgcggcggc gaaggcgtga tcgtgcgcta cgaccccgac
     1021 cgcgggttcg gtttcatcgc ccccgactcc ggaggcgacg acctgttcgt gcacgtgtcg
     1081 gtcatccgtg gctccgatca cctgtaccag ggcgatcggg tccggttcca ggtgcgtcag
     1141 agcgatcggg gcccgcaggc ggaccgcgtc gaactgatct gaacggtcag gcgtcctcgg
     1201 cgagggcgac gtcgaccatc agctcgtctg cgagcgactc catcgccgcc cgcagctcac
     1261 cttcgtccgc cccggcaggc gcggccagca cggcgtgagc ctcgaagagc atgccgccgg
     1321 ccatcggggc ctcgctgacg aaggtctcca gctcgacgat gccgatcccc cgcgccgaga
     1381 gcagcgccga cacctcggcg acgatgccgg ggcgatcggc cccgaccagg tcgagggtga
     1441 gatggtgcgt cggcgcctcg ctcgaggcgc tcgtgcgcgt ggtcgagacg gtgatgccct
     1501 cggccgagag cccgtcgagg tcggccacga gcccgtcgac accgtccgcc gggacgacga
     1561 cctcgacgat gccggcgaac ttgccggcca gctgcgacaa ctggctgcgc tgccacgtgg
     1621 cgccccgagc ggcgaggctg gccgacagcg tcgccaccag cccgggtcgg tcgtcactga
     1681 tgaccgtcag caccaaggtc tccatgccac gtcaccctac ggccgagagg acccccctgc
     1741 ccagagctga ccccgagcgt tcacctgccc ccggtagcgt ggcgcgcaat gagtactcca
     1801 cacctgatcg cactcgacat cgacggcacg accgtgaacc acgaaggtgc cctgtcgccc
     1861 gcggtccgcg atgccgtgac cgccgtcgcg gatgccggcc accacgtcac catcgccacc
     1921 gggcggggga tcatcggcac cctgccgatc ctcgaccgac tcggcctgac caccgggtac
     1981 gcggtgtgcg ccaacggcgc catcaccctg cggctggacc cgacgtcgga tgccgggtac
     2041 gaggtcgtcg agtcggtcac cttcgacccg gcgccggccc tgaccgccct gcgcgaacac
     2101 ttcccctcgg cgctggtcgc ggtcgaggaa ctcggcgtgg gcttcaaggt gagcgcgccg
     2161 ttccccgacg gtgagctgga cggcatcccc gaggtcgtga ccttcgacga gctgatgtcg
     2221 acgccggtca cccgcgtgac gctgcgtgac cccagtgcga cgagcgagga gttcatcgag
     2281 cgcacggcac gcatcggcct gcacggggtc gagtacgccg tcggctggag cgcgtggctc
     2341 gacatcaccc cggagggggt ctccaagggg gcggcgctcg aggtcatccg gcgctggctg
     2401 gccatcgagc cgcagtacac cgtcgctgtc ggagaccagc gcaacgactt cgagatgctg
     2461 cgctgggcgg ctcgcggggt ggccatgggc aacgccccgg acgaggtcaa ggcggtcgcc
     2521 gacgaggtga cgggtgacgt cgacgacgac ggtctcgtcg acgtgctgac ctcactgctc
     2581 tgacctcacc gctctggcgc cgccacgagc gcgagccgaa ctcgcgcgca cgtagcgcgc
     2641 cacggcaccc gcgctgatgt catggtgagc ggatggacag caccgacgtc agcagcgccg
     2701 cccgcaaggc cggcgaccac cccgtcgtcg agaagggcgc gcgcatcggg tacgcgatgt
     2761 ccggcgtcat ccacttcatc atcggctgga tcgccctcaa gctcgcgtgg ggcatcggcg
     2821 gcggctccgg tgacgccgac acctccggag ccctgaagac cgtcgcgagc agcggtggcg
     2881 gcccgctgat gctcggcttc gcggtcctcg gcttcctcat gctggccgtg gcgctggcgg
     2941 cggcagcgat cgtgggcggc gagaccagcg accgcatcaa gtcggccgcc aaggccgtga
     3001 tgtacacgag cttcgcctgg tcctcgttcg cgatcgcgcg cggtgcgtcg aactccgacg
     3061 aggcgaagac cgacgacgtc accgcgcagg tcatggggat gccgggtggt cgctacatcg
     3121 tcggtctcgt gggtctcgtc gtgctcgggg tcgccgggta ccacgtctac aaggggtggt
     3181 ccaagaagtt cctcgaggac ctcgaggaac accccggcga ctgggccgtg acggccggac
     3241 gactcggcta cgtcgccaag gggtgtgcgc tcttcgtcgt cggactgctg ttcctgctcg
     3301 cggcctggca cgccgactcc gaggaggcgg ccgggctcga cggggccctg acgacgatcc
     3361 gcgacctcgc cttcggtccg tacctgctca cgctggtggc cgcggggatc gcgtcgtacg
     3421 gcgtctactc cttcgcccgc gcccggtacg cccgcctctg agcgaaacgc aggagctcag
     3481 atcgcacgca gtaattgccg cgagcgatct gaactcctgc gttccaaggg cctgccgcgc
     3541 gcctccggca tcgtctacgt catcggtcgg cttgcgaccg cagctttgcg cccgaggtag
     3601 agccaggcca acacggcgca caccaccccg ttgaggatgc cgaacaggat gtcggtcacg
     3661 tggtgcgcgc cccggtagag ccgtgcgtac gcgacgaacg ccggggcgag gacgcaccac
     3721 gtgaccgcca gccggcgcac gagcgggttg tcgatccgcg tcgccatcag tcccagggcc
     3781 acgtacagcg ccgtcgaggc accgacgtgc ccgctcggga agctcgacgt cggaggagtc
     3841 gggtcgagtc gctccacggt cggccgggcg cggccgacga cgtgggtggc gatgacgaac
     3901 acggtggcct ggaggctgat cgcgatgccg ggcaccacgg cgtaccacca ctgcttcgtg
     3961 cgccaccaga ccaggccgat gacgatgagg cacacgccga tgacgtactc ggtgttgccg
     4021 atcgaggaca tcaggtgggt gatcgagttc cagagcccgt cgcgtgcgtc ctggaactcg
     4081 cggttgatct gcttctcgtc ctccgagagc tcgaacagcg ggcccttgag caggtagccg
     4141 aacccgatga tgaccgcgag cagggcggtc gctggaagca cggcccgcac cccgaggtca
     4201 cgcagcgcgg ccttgagtgt cggccggctc tggtcgtcgt cccaccgagt gaggaacccc
     4261 atcactgacc ctcctgtcgt cggtagccag cgtaggaggc cagcaccagg ccgaccccgg
     4321 tgatgacgcc gacggtcacg tctgagggga agtgcacccc gagcaggacg cggtcggatg
     4381 ccgtgaccgt caccatcgcg accgccgccg tgacggcgac gactcgtgac gccgtcgacc
     4441 ggagcagcgg ccagaggagg agcacgacga tcgttgccca cgcggccgag ttcagggcgt
     4501 ggccggaggg gaaggagtag ccggggctgg tcgacaccgg gtcctcgagc accgggcgag
     4561 cccgctggaa caggtacttg gcgccgagcc ccaccatcca cgcgagcatc atcgtgatga
     4621 aggcccacgc ggcccgggtg cgcaggccct tggccagcca ggcccacagg cacagggcgg
     4681 tgccgaccag gtagagcttg aacggctggg tcagctcctg ccagcgcacg aggaagtcgg
     4741 tcaacccctc gcgcgggcgc gtgtacgcgg tggcccgctc gatgatggcg tcgtcgagcc
     4801 ggatgagcgg gtcccactcg ctgcgcacga gcagccccac cccgaagagc acggctgcga
     4861 cgggaagggc gtacgtcagt gcgcgcacca gccgttgtgc cctcgtcggt ggcgcagggg
     4921 cagtgagggt gtcatccacc ggctatgtct accccgatca ggtgcctcca cgacctccga
     4981 gagacagacg aactgctcac cacccgccat cctcgtggga tgctggggcc gccatggccg
     5041 agctgcacgt gacctcagcg tccaaccccc gactcaaggc cgtgctggct ctgcgtcgcc
     5101 gtcgggtgcg cgaggacgag ggtctgaccg tcgtcgacgg gtacgaggag ctgaccctgg
     5161 cgctcgacgc cggtgtggtg ccccggaccg tgctgcactg ccccgagctc atgctcgacc
     5221 ccgccgccca gggtgagctc gtgcagcggg ctcgggacct cggggcgtcg acggtccggc
     5281 tcagccgggg agcgttcgag aaggtcgcct accgcgaggg gccggacggg ttcctcgccg
     5341 tcgtccccgt ggccggggtg gcgctcggtg acctcgacct gccgcccgac ccgctcgtgc
     5401 tcgtctgcga gggcctcgag aagccgggga acctcggggc cgtgctgcgc accgcggatg
     5461 ccgcgggcgt cgccgcggtt gtcgccgccg accccgtcac cgactggggc aaccccaacg
     5521 tcgtgcgggc cagcaagggc acggtcttct ccgtccccgt cgcctccgcg acgaccgagg
     5581 aggtcgtggt gtggttgcgg cgcaacggca tccgactcat cgcgaccacc cccgacaccg
     5641 acaccgcgca caccgacgtc gactaccgcg gcggcgtcgc gatcgcggtc ggcaccgaga
     5701 agcagggcct gaccgacgcg gcgctgacgg ccgcggacgc gcgggtgcgc atccccatgg
     5761 tggggcgggt caactccctc aacgcggcca cggcggctgc cgtcgtcgtc tacgaagcgg
     5821 tgcgccagcg ttcctcctga ggcgggcgtg gtcggtgtga ccgccggatg gtcacgctcc
     5881 tgtcacggcg tcggcgtgag cccggcctgt gtcaagagca ccccttcgcg tgtgcgacga
     5941 tgaacggcat gcgagttgac cacgtcgtct atgccgccga gcgcgacggc ctcaaggcca
     6001 cggcgcagcg tctcgcggag cagatcgggg tgaccccggt cgatgggggc atccacccgc
     6061 gcttcgggac acgcaacatc atccttccgc tcgcccacga gcggtacgtc gaggtcgtcg
     6121 aggtgctcga ccacccggcc agcgacaagg ctcccttcgg ccaggccgtg cgtgcgcgtt
     6181 ccgaggccgg cggtgggtgg ctcggctggg tcgtgcaggt cgacgacatc accgagcagg
     6241 agtcccgcct cggccgcgag gccgttgacg gcaaccgcca ccgccccgac ggggtcgagc
     6301 tgcgctggcg ccagctgggc atcaagggcc tgcagtccga cccgcagctg ccctacttca
     6361 tcgagtgggc gcagggggtg ccgcacccct cgggtgacgc cgccaccgag gtgaccatcg
     6421 agtcgctcca gatcgccggc gaccccgacc gggtgcgcga gtggctcggc ctgcccgccg
     6481 actcgacgtc gacggtcatc gacttcaagt tcgtctcccc gcacggcacg cccggcctca
     6541 tggccgtcac gttcgacacc ccgaacggcg acgtcaccat ctgacccggt cgcggaaggg
     6601 ctctctcagc cctcgcgcga ccagcggcgc agccgcaggc tgttgctcac gacgagcacg
     6661 ctggaggcag ccatggcgcc ccccgcgatc atcgggttga gcagcccggc catcgccagc
     6721 gggatcgccg cgatgttgta gccgaaggcc cagccgaggt tctgcttgat cgtgcgcagg
     6781 gtcgcccggg acagctcgat cgcgtcgacg acggcgggta gttcggagcg cagcagcacg
     6841 atgtcggccg agtccgcggc gacgtccgtg cccgtcccca tggcgatgcc gaggtcggcc
     6901 tgggccagcg ccgcagcatc gttgacgccg tcgccgacca tcgcgacgac cttgccccgc
     6961 ttctgcaggt cggcgatcat cgcgtgcttg tcctcggggc ggacgtcggc gatcacccgc
     7021 ccgaccggga tgccgacgtc acgggccacg gtcagcgcgg cctggtcgtg gtcaccggtc
     7081 aacaggtacg ccggcaggcc cagttcggcg agatcgcgca cggcccgctg cgagctgcgg
     7141 cgtaccgtgt cggccaccgt gagcgaggcg tgggcacgcc cgccccagcc gacccagacc
     7201 gtcgtgccgt cgacggtggg acgggcgagc tcctgcggca cgtcgtcgaa gaggtcggcg
     7261 cgcccgacgg tgacctcggt gcccttgatg atcgcccgtg ctccggctcc cggcagcgac
     7321 acgaagtcgg tggcgcgcgg aacgtcgatc gcacgactgg cagcggcggt gacgactgct
     7381 cgggcgacgg ggtgctcgct gcccgtctcg acggcggctg ccgctcgcag tgcctggtcc
     7441 ttgggcaggc gcccggcggt tgcgacgtcg acgagggtca tgtcgccgct ggtgaccgtg
     7501 ccggtcttgt cgagcacgac ggtgtcgatg cggcgggtgt cctcgaggac gtcggcaccc
     7561 ttgatgagga tgccgaggcg ggcgcctcgc ccggtgccga cgaggagcgc ggtgggcgtg
     7621 gccaacccca aggcgcaggg gcaggcgatg acgagcacgg cgatcgcgat ctcgagggcg
     7681 cgtgcggggt cgccggtgcc gatccaccag ccgaggaacg tcagtgccgc gagcacgagc
     7741 acgatcggca cgaagatcgc cgagacccgg tcggccaggc gctggatggg tgccttggtc
     7801 gtctgcgccc gctccacgag ccgggcgatg ccggccaggg tggtgtcggc gccgacccgg
     7861 gtggcctcga cgacgagccg gccggtggtg ttgatgctgc cgccgacgag gtcgtcgccg
     7921 atggcgacgt cgaccggcag cgactcgccg gtgacgaggg aggcgtcgac ggcgctggag
     7981 ccctcgacga tccttccgtc ggtcgcgacc ttctcgcccg ggcgaacgac gaagcggtca
     8041 ccggcgccga gctggtcgac ggggatccgc acctcggtcg tcgcccgggt cgtggggtcg
     8101 acccgctcga ccgcgacgtc cttggcgccg agcgacatca ggccgcgcag ggcatcgcgg
     8161 gtgcggtccg tggcgcgggc ctccatccag cgtccgatga gcaggaaggt ggtgaccacg
     8221 gtggcggtct cgaaccacag gtgggtggtg ccagcggcat ccacgccgtt tgtcgtgccc
     8281 atgtcggcca tggtgctggc gcgaccggtg aggacgacga ctgccgacca caggtaggcg
     8341 gtgagcacgc cgatcgacac gagggtgtcc atcgtcgagg cgccgtggcg cgcgttgagg
     8401 gcggctgcgc ggtggaaggg ccagccggcc cacagcacga cgggggtcgc gagggcgagc
     8461 tgcacccacg ggctggagtc gaggtggatg ccggggacca tcgccaggat gaccaccggg
     8521 acggtgagca ccgccgcgac gagggcacgc cgcttcaggg cgtcctcggg ggcgacggcg
     8581 tggtcgaggt ggttctcgtg cgtccccgaa tcgaccaata ggtcactcgg ggtacgggcc
     8641 gatccgccgc cgggcgtacc tgtaccgacc aataggtcac tcggggtacg ggtcgacccg
     8701 gcggcctccg gctgagggcc gccgcggacg acctcggcgc cgtaaccggt ggcgcgcacg
     8761 gtgcgcaaca ggtcctcgac ggtgaccgcc ccggcatagc gcacggtggc cttctcggtc
     8821 gcgaggttca ccacggcttc cacgccgtcg agcttgccga gcttcttctc gatgcgggcc
     8881 gagcacgacg cgcaggtcat gccggtgatg gcgaggtcga cctcgcgcac ctcgtcgtgg
     8941 tcgtgctgcg ggcggtcggt ggtctgggtc atgtcagctc ctcaccagcc gggcgatcgc
     9001 ggcggtggcc tcggcgacct tgacggcggc ggcctcgtcg gactcacgag cggcgtcgac
     9061 gacgcagtgc tcgatgtgct cctcgagcag gccgaggctg accgcctgga gggctcgggt
     9121 cacggcgctc acctgggtga ggacgtcgat gcagtacgcg tcggactcca ccatccgcgc
     9181 gatgccgcgc acctgcccct cgatgcggcg cagcctcttg agatggtcat ccttgtgccc
     9241 cgcgtagccg ggggcgacgg tggcggtgtg ctcggtcatg tccaccccaa catgataccc
     9301 ctagggggta ttcctgcggg tcggaggggt cagtcgagac gttccgtcac cggacgcccc
     9361 cggagcca
//
LOCUS       DAOSOC010000092         9325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7329_length_9325_cov_8.425458, whole genome shotgun sequence.
ACCESSION   DAOSOC010000092 DAOSOC010000000
VERSION     DAOSOC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7329_length_9325_cov_8.425458"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2275)
                     /locus_tag="PL014_08155"
     CDS             complement(<1..2275)
                     /locus_tag="PL014_08155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOT57100.1"
                     /translation="MPSLTDLLHAAVGGVGGTERPGQVEMAQAVADAIDTKSHLLVQA
                     GTGTGKSLAYLVPAVQHAVDTGIPAVVATATLALQAQIVDRDMPRLADAVAPLLGRRP
                     TYALVKGRRNYLCAHKVEGGFPDDDADSLMSLGMVDQQASRLGAEVVRLREWAEVTES
                     GDRDELVPGVSERAWRQVSVSAQECLGSRCPMVTECFVERSRAAAADVDVIVTNHSFM
                     AIDAFEGRQMLPEHDVLIIDEGHELVDRVTSTITDEVTPGMIRAAAKRAGRQADASST
                     MEEAADLLESVLEPAPEGRLTGIPDSLAMALGRVRDTARTVQSELKPQQGEEVDAGRQ
                     VARAAVDEVFENACRILEERELDVVWLSRDPRRGPVLRVAPMSVAMLVRDKVFQERTV
                     VLTSATLELGGSFDAVAGTIGLRGEGAPRWRGLDVGSPFDYPKQGIAYVAQHLPPPGR
                     DGLAGETLDEIEALVRAAGGRTLGLFSSMRAAKEATEAMRERFADKEGEIAFLCQGED
                     QISTLVRQFARDPRVCLFGTLSLWQGVDVPGSSCQLVIIDRIPFPRPDDPLASARSQA
                     IARMGGNGFMAVSATHAALRLAQGAGRLIRRSDDRGVVAFLDNRMMTARYAGFLQRSL
                     PPFWPTSDRAMVLSALARLDEIADPPLPVAEPALRGLTGALAAPVGDTARGADAVAPV
                     PADRPVAEAPPPPESSRTAVTHGHAWTEQEDEELRDGIELGLTLDELSESMELPTEVL
                     AARLAGLGLEAGKGATLT"
     gene            2450..4153
                     /locus_tag="PL014_08160"
     CDS             2450..4153
                     /locus_tag="PL014_08160"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOT57101.1"
                     /translation="MTADTTSAPFPSDIEIAEAAEITPITKLAQERLGLPAEALIPYG
                     HTKAKVDLAHIRSLADEPEGTLILVTAISPTPAGEGKTTTSVGLADALSRIGKKSMVA
                     LREPSMGPVFGIKGGAAGGGYAQVVPMTDINLNFTGDFASIAAANNLLSALIDNHIHH
                     GNALGIDPRTITWKRVVDLNDRALRDIVVSLGGVANGFPREDGFDIVVASEVMAIFCL
                     ATSLADLKERLGNIVIGYTRDKKPVTARDLKANGAMTVILRDALAPNLVQTLEHTPAM
                     IHGGPFANIAHGCNSVMATRTALKLADYVVTEAGFGADLGAEKFIDIKCRTSGLRPSA
                     VVVVATIRALKYHGGVALADLKTEDVDALTRGMENLRKHLRNVKDVYGLPPVVAINRF
                     PTDTDAEVAALIDLVRAEGARAYEATHFSDGGAGAEALAHGVLEAIEAGEGEMQFVYA
                     DELSLADKAEAIATKVYGAAGVTFDSKAAKRLAQIEADGYSGLPVCMAKTQSSFSTDA
                     TRRGAPTGHTVNIREVRLAAGAGFVVMVTGDIMTMPGLPRVPASDHIDIDEDGNIVGL
                     S"
     gene            complement(4146..>4514)
                     /locus_tag="PL014_08165"
     CDS             complement(4146..>4514)
                     /locus_tag="PL014_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HOT57102.1"
                     /translation="GNSNWRGPVWFPINVLLIDGLRGHATAAGGSLQVEYPTGSGSTR
                     GLGEAADDLEQRLVSLFRPDADGRRPGTPRDHPSGPLWDVHPTFAEYFDGDTGRGCGA
                     SHQTGWTALVAHLICARPLS"
     assembly_gap    4515..4614
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4615..6272)
                     /locus_tag="PL014_08170"
     CDS             complement(<4615..6272)
                     /locus_tag="PL014_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HOT57103.1"
                     /translation="MPEGPLTDEQVRLRHSPGDDAKEYWWALDATPTHSWAQHLYRYP
                     QAAFPYATLVEENARRGPDAPELQLADTGVLQESRFFDIVTTHAKVAPDDVCMVIEAT
                     NHGPDAAPLDLVPQLWFRNTWSWGRDDRLPWLRLVDADDTLAPGTTVVQAEHGWLGRY
                     VLVAEGAPDVLFCDNETNVAAVFGPDAGASLSAYPKDGIGRAVVDGDDSGTNPAATGT
                     KVGLVYRFESVAPGETVRVQLRLRADHQVERPFGRSFAAVLEDRRREADEFYDTVIPS
                     DVSDEDRHISRRAFAGLNWGKQLYRYSVKEWLDGDPAGPPAPPGRRARTARNRAWSQL
                     ALADVISMPDEWEYPWFATWDLAFHCVAIAHMDPAFAKNQLLLLVREWAQHPNGQLPA
                     YEWDFGDVNPPVHAWAAWHVHQLDGGTDRAFLVRIFTKLMFNFSWWLNRKDSDGSNLF
                     EGGFLGMDNISVFDRSRDVPPGWRLEQSDATSWMAFFSLSMLRIALELSREDQAFDGL
                     ATTFLEHFLSVAEAMETFGTDDVSLWDEADGFFYDVLVGPDGRS"
     assembly_gap    6304..6403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6404..6612)
                     /locus_tag="PL014_08175"
     CDS             complement(<6404..6612)
                     /locus_tag="PL014_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57104.1"
                     /translation="MSQFTESSPADQHRVASDQLVAIADRVSDWDAPTPVREWVARDI
                     PEHLTWLGGFLGGLGVSVDIPHHDDP"
     gene            6699..8309
                     /locus_tag="PL014_08180"
     CDS             6699..8309
                     /locus_tag="PL014_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT57105.1"
                     /translation="MTPDAAIVLTAAFAAGLIARVLRLPPLLGFLAAGFALAGADSVG
                     SGADLGRRPVIDLLADLGVTLLLFAVGLHLDVRRLLRREVWLTTGVHSLLMVGIGVGF
                     LTALSALGVAEASGLDLRALALIALALSFSSTVFVVKVLDERNESRSRYGQIAIGVLV
                     VQDVFAVVFVAVSHGSPPSPWAFGLLLLIPARRVIVALWERAGRAELQVLFGVLMAFV
                     PGYALFDALGLKGDLGALAMGMLLAGAARSDDLAKSIFGVKELLLVAFFLSIGLHGVP
                     DLQQVLTAALLLLLLPVQTLAYVVLLSMLRMRRRTAVLTALVLANSSEFALVVMATAQ
                     DAGLVTERWVTTTAVTVALSFVLSSAVNAKAEQISDAIERRWPDPDPESLDEAERPIP
                     LHDVDALVLGMGRVGSAAHRRLTERGLRVLGIEHDEARVARLQAQGVEVVLADATDSS
                     LWRRLVAVRTLRTVVLAMPFHEANLATIEVVRARSFTGTVAAVARYDDEVTELHANGA
                     NTVLHVYSGSGVALADAALDGPEEALQP"
     gene            complement(8337..>9325)
                     /gene="hflX"
                     /locus_tag="PL014_08185"
     CDS             complement(8337..>9325)
                     /gene="hflX"
                     /locus_tag="PL014_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT57106.1"
                     /translation="VKVIDRTALILDIFAQHAKSREGKAQVELAQLQYLLPRLRGWGE
                     SMSRQAGGQAAGGQGMGSRGPGETKIELDRRRINTRIAKLRREIKEMKTVRDTKRGSR
                     KANAVPSVAIAGYTNAGKSSILNRLTGAGVLVENALFATLDPTVRKAETLDGRLYTLA
                     DTVGFVRALPHQLVEAFRSTLEEVAEADLLLHVVDGSHPDPEGQISAVRAVLADVDAT
                     DVREVIVINKADVADPEVIARLQRHEKHSIVVSARSGEGMEELRALIGEELPHPDVEV
                     DVLVPYDRGDLVSRLHEQGDVLESRHEAEGTRVRARVHANLAADLADMVAPA"
BASE COUNT         1264 a         3304 c         3230 g         1327 t
ORIGIN      
        1 aggtgagggt ggctcccttg ccggcctcca gcccgagacc ggcgagccgg gcagcgagca
       61 cctccgtcgg cagctccatc gactcactga gctcgtcgag ggtgaggccg agctcgatgc
      121 cgtcgcgcag ttcctcgtcc tcctgctcgg tccacgcgtg gccgtgggtg accgccgtcc
      181 gcgaggactc cggcggcggc ggcgcctcgg ccaccgggcg atccgccggc acgggcgcca
      241 cggcatccgc accccgcgcc gtgtcgccca ccggcgccgc gagcgcgccc gtcagcccgc
      301 gcagggcggg ctcggccacc ggaagcggcg ggtcggcgat ctcgtcgagg cgcgccagcg
      361 cggacagcac catcgcccgg tccgaggtcg gccagaacgg cggcagcgag cgctggagga
      421 agccggcgta ccgagcggtc atcatccggt tgtccaggaa cgcgacgacc ccgcggtcgt
      481 ccgagcgccg gatcagccga cccgccccct gggccaggcg cagcgccgcg tgggtggcgg
      541 agacggccat gaacccgttg ccacccatcc gcgcgatggc ctgggaccgg gccgaggcga
      601 gcgggtcgtc gggccgtggg aacgggatgc ggtcgatgat gacgagctgg caggacgagc
      661 ccggcacgtc gaccccctgc cacagcgaga gcgtgccgaa caggcacacc cgcgggtcac
      721 gggcgaactg ccgcaccagc gtgctgatct ggtcctcgcc ctggcagagg aacgcgatct
      781 cgccctcctt gtcggcgaac cgctcgcgca tcgcctcggt ggcctccttg gcggcgcgca
      841 tcgaggagaa cagcccgagc gtgcggcctc cggcggcgcg cacgagcgcc tcgatctcgt
      901 cgagggtctc ccccgccaga ccgtcgcgcc cgggcggcgg caggtgctgg gcgacgtagg
      961 cgatgccctg cttggggtag tcgaacggac tgccgacgtc gagcccgcgc cacctcggcg
     1021 ccccctcgcc gcgcaacccg atggtgccgg ccacggcatc gaaggacccg ccgagctcga
     1081 gcgtcgccga cgtgagcacg acggtgcgct cctgaaagac cttgtcgcgc acgagcatcg
     1141 ccacgctcat cggggccacc cgcaggacgg gcccacggcg cgggtcacgg ctcagccaga
     1201 cgacgtcgag ctcacgctcc tcgaggatgc ggcaggcgtt ctcgaacacc tcgtcgacgg
     1261 cggcgcgcgc gacctgccgc ccggcatcca cctcctcccc ctgctgcggc ttgagctcgc
     1321 tctgcacggt tcgggccgtg tcccgcaccc gaccgagggc catggcgagc gagtcgggga
     1381 tgccggtcag gcggccttcg ggagccggct ccagcaccga ttcgagcagg tcggcggcct
     1441 cctccatcgt cgacgacgcg tcggcctggc gcccggcccg cttggcggcg gcccggatca
     1501 tgccgggggt cacctcgtcg gtgatggtgg acgtcacccg gtcgacgagc tcgtggccct
     1561 cgtcgatgat gaggacgtcg tgctcgggca gcatctgacg gccctcgaag gcgtcgatcg
     1621 ccatgaacga gtggttggtg acgatgacgt cgacgtcagc ggcggcggca cgcgagcgct
     1681 cgacgaagca ctcggtgacc atcgggcagc ggctgcccag gcactcctgc gcactgaccg
     1741 acacctgccg ccaggcccgt tcggacactc ccggcacgag ctcgtcgcgg tcgccggact
     1801 cggtgacctc ggcccactcg cgcaggcgca cgacctcggc cccgagcctg gaggcctgct
     1861 ggtcgaccat cccgagtgac atgagcgagt cggcgtcgtc gtcggggaag ccgccctcca
     1921 ccttgtgcgc acacaggtag ttgcggcgcc ccttgaccag cgcgtaggtg ggacgacgac
     1981 ccagcagggg cgccacggca tccgcgagcc gtggcatgtc gcggtcgacg atctgcgcct
     2041 gcagcgccag cgtcgccgtc gcgacgacgg cggggatgcc ggtgtcgacg gcgtgctgga
     2101 cggcgggaac gaggtaggcc agcgacttcc cggtgccggt gcccgcctgg acgagcaggt
     2161 ggctcttggt gtcgatggcg tccgcgacgg cctgcgccat ctcgacctgt ccgggccgct
     2221 cggtgccgcc cactcccccg acggcggcat ggaggaggtc ggtcagcgaa ggcaccagag
     2281 cagggtatgc cgtcgcgccg gtggcctgcg tgtgcccgtc cacaggctgc cgggcccggg
     2341 tgctcgtggc tgggcagaac cgccgcgatg ttgcgcatcg tgcgccgggt tgcgccatgt
     2401 ggcgccaagg tgatgacagg gaagcccccg tggtgcttga ctggcgcctg tgaccgctga
     2461 caccacctct gcccccttcc cgtccgacat cgagatcgcc gaggcggccg agatcacccc
     2521 gatcacgaag ctcgctcagg aacggctcgg cctgcccgcc gaggcgctga tcccctacgg
     2581 ccacacgaag gccaaggtcg acctcgcgca catccgcagc ctggccgacg agcccgaggg
     2641 aacgctgatc ctcgtcaccg cgatctcccc caccccggcc ggtgagggca agacgacgac
     2701 gagcgtgggc ctggccgacg ccctcagccg gatcggcaag aagtcgatgg tggccctgcg
     2761 cgagccctcg atggggccgg tgttcggcat caagggcggt gccgcgggcg gcgggtacgc
     2821 ccaggtcgtg ccgatgaccg acatcaacct gaacttcacc ggcgacttcg cctcgatcgc
     2881 cgcggcgaac aacctgctgt ccgcgctcat cgacaaccac atccaccacg gcaacgcgct
     2941 cggcatcgac ccgcggacca tcacgtggaa gcgcgtcgtc gacctcaacg accgcgccct
     3001 gcgcgacatc gtcgtctcgc tcggtggggt ggcgaacggc ttcccgcgcg aggacggctt
     3061 cgacatcgtc gtcgcctccg aggtgatggc gatcttctgc ctcgcgacgt ccctggccga
     3121 cctcaaggag cgcctgggca acatcgtcat cggctacacc cgcgacaaga agccggtgac
     3181 ggcccgcgac ctcaaggcca acggcgcgat gaccgtcatc ctgcgcgacg cgctggcccc
     3241 caacctcgtg cagaccctcg agcacacccc ggcgatgatc cacggcggcc cgttcgccaa
     3301 catcgcacac ggctgcaact cggtcatggc cacccgcacg gccctcaagc tcgctgacta
     3361 cgtcgtgacc gaggccggct tcggcgccga cctcggcgcg gagaagttca tcgacatcaa
     3421 gtgccgcacc tcagggctgc gcccgagcgc ggtcgtcgtg gtggcgacca tccgcgccct
     3481 gaagtaccac ggtggcgtcg ccctggccga cctgaagacc gaggacgtcg acgccctcac
     3541 gcgtggcatg gagaacctgc gcaagcacct gcgcaacgtc aaggacgtct acggcctgcc
     3601 gcccgtggtc gcgatcaacc gcttccccac cgacaccgac gccgaggtcg ccgcgctcat
     3661 cgacctcgtc cgggccgagg gtgctcgcgc ctacgaggcg acgcacttca gcgacggtgg
     3721 cgccggtgcc gaggcgctgg cccacggtgt gctcgaggcc atcgaggcgg gcgagggtga
     3781 gatgcagttc gtctacgccg acgagctctc gctggccgac aaggccgagg ccatcgccac
     3841 gaaggtctac ggcgcggccg gggtcacgtt cgactccaag gccgccaagc ggctcgcgca
     3901 gatcgaggcc gacggctact ccggcctgcc ggtctgcatg gccaagacgc agagctcgtt
     3961 ctccaccgat gccacccgcc gcggcgcccc gaccgggcac accgtcaaca tccgcgaggt
     4021 ccgcctggcg gcgggcgcgg gcttcgtcgt catggtgacc ggcgacatca tgacgatgcc
     4081 cgggctgccc agggtgccgg catccgacca catcgacatc gacgaggacg gcaacatcgt
     4141 cggcctcagc tgaggggcct ggcgcagatc agatgggcga cgagggcggt ccagccggtc
     4201 tggtgcgatg cgccgcagcc gcgcccggtg tcaccgtcga agtactcggc gaacgtcggg
     4261 tgcacgtccc agagcggccc gctcgggtgg tcccggggcg tgccgggtcg gcgcccgtcc
     4321 gcgtcggggc ggaacagcga cacgagtctt tgctccaggt cgtcggccgc ctcccccagg
     4381 cctcgggtgc tccccgaacc ggtggggtac tcgacctgga ggctgccccc ggccgccgtg
     4441 gcgtggccgc gcagcccgtc gatgagcagc acgttgatcg ggaaccacac cggcccacgc
     4501 cagttcgagt tgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcggag
     4621 cgaccgtcgg gcccgacgag cacgtcgtag aagaagccgt ccgcctcgtc ccacagtgac
     4681 acgtcgtcgg tgccgaaggt ctccatggcc tcggcgacgg acaggaagtg ctcgaggaag
     4741 gtcgtcgcca gcccgtcgaa ggcctggtcc tcgcgactga gctcgagggc gatgcgcagc
     4801 atgctcagcg agaagaacgc catccagctc gtcgcgtcac tctgctccag gcgccaaccc
     4861 ggcggcacgt cgcgcgagcg gtcgaacacc gagatgttgt ccatcccgag gaacccgccc
     4921 tcgaagaggt tggacccgtc ggagtccttg cggttcagcc accaggagaa gttgaacatc
     4981 agcttggtga agatgcgcac gaggaaggcc cggtcggtgc cgccgtcgag ctggtggacg
     5041 tgccaggccg cccacgcgtg caccggcggg ttgacgtccc cgaagtccca ctcgtaggcc
     5101 ggcagctggc cgttggggtg ctgggcccac tcacgcacga gcagcagcag ctggttcttc
     5161 gcgaacgccg ggtccatgtg cgcgatggcc acgcagtgga aggcgaggtc ccacgtcgcg
     5221 aaccaggggt actcccactc gtcgggcatc gagatgacgt cggcgagggc gagctggctc
     5281 caggcgcggt tgcgcgccgt gcgcgcccgg cgtcccggcg gcgcgggcgg cccggccggg
     5341 tcgccgtcga gccactcctt gaccgagtag cggtacagct gcttgcccca gttcagcccg
     5401 gcgaacgctc ggcgggagat gtgccggtcc tcgtcgctga cgtcgctcgg gatgacggtg
     5461 tcgtagaact cgtcggcctc gcgcctgcgg tcctcgagca ccgcggcgaa cgagcgaccg
     5521 aacggccgct ccacctggtg gtcggcccgc agccgcagct gcacccgcac cgtctcaccg
     5581 ggtgcgaccg actcgaaccg gtagaccaac ccgaccttcg tgccggtcgc cgcggggttg
     5641 gtgccgctgt cgtcgccgtc gacgaccgcc cgcccgatgc cgtccttggg gtaagccgac
     5701 aggctcgccc cggcatccgg cccgaacacc gcggccacgt tggtctcgtt gtcgcagaac
     5761 agcacgtccg gggcaccctc ggcgacgagg acgtaccgcc cgagccagcc gtgctcggcc
     5821 tggaccaccg tggtgccggg ggccagcgtg tcgtcggcgt cgacgaggcg cagccacggg
     5881 aggcggtcgt cgcggcccca ggaccacgtg ttgcggaacc acagctgcgg cacgaggtcg
     5941 agcggggcgg catccgggcc gtggttggtc gcctcgatga ccatgcagac gtcgtccggg
     6001 gcgaccttgg cgtgcgtcgt cacgatgtcg aagaagcggc tctcctggag caccccggtg
     6061 tcggcgagct ggagctccgg ggcgtccggg ccgcgccgag cgttctcctc gacgagcgtg
     6121 gcgtacggga aggcggcctg cgggtaacgg tagaggtgct gggcccacga gtgcgtcggg
     6181 gtggcatcca gcgcccacca gtactccttg gcgtcgtcgc cggggctgtg gcgcagccgg
     6241 acctgttcgt cggtgagtgg gccctcaggc accggggctc cagtgcggat cacgaccgag
     6301 cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngggtcgt cgtggtgcgg
     6421 gatgtccacg ctcacaccca gaccgccgag gaagccgccg agccacgtga ggtgctcggg
     6481 gatgtcacgg gccacccact cgcgcaccgg ggtcggcgcg tcccagtcgc tcacccggtc
     6541 cgcgatcgcg accaactggt cgctggcgac ccggtgctgg tcggccgggg acgattcggt
     6601 gaactgggac acggcatcct cctgcggggg cggtggccgc ccagcctgcc atgctcgggc
     6661 agactcacga ccggagcacg gcagcgaagg aggcatggat gacgccggat gccgcgatcg
     6721 tcctcaccgc agccttcgcg gccggcctga tcgcccgggt gctgcggctg ccacccctgc
     6781 tgggtttcct cgccgccggg ttcgcgctgg ccggggccga ctcggtcggt tccggcgccg
     6841 acctgggcag gcgaccggtg atcgacctgc tcgccgacct cggggtcacc ctgttgctct
     6901 tcgccgtcgg ccttcacctc gacgtccggc ggctgctgcg ccgagaggtg tggctgacca
     6961 ccggggtgca ctcgctgctc atggtcggca tcggcgtcgg cttcctcacc gcgctgtccg
     7021 cgctcggcgt cgccgaggcg tccgggttgg acctgcgcgc cctggccctg atcgccctgg
     7081 ccctgtcgtt ctcgagcacc gtgttcgtcg tcaaggtgct cgacgagcgc aacgagtcac
     7141 gctcgcgcta cgggcagatc gcgatcggcg tcctcgtcgt gcaggacgtc ttcgccgtgg
     7201 tcttcgtcgc cgtgtcccac ggcagcccgc cgagcccgtg ggcgttcggc ctgctcctgc
     7261 tgatccccgc ccggcgggtg atcgtcgcgc tctgggagcg agccgggcgg gccgagctgc
     7321 aggtgctgtt cggggtgctc atggccttcg tcccggggta cgccctcttc gacgcgctgg
     7381 ggctcaaggg tgacctcggc gccctggcca tgggcatgct gctcgccggc gcagcgcgct
     7441 ccgacgacct ggcgaagtcg atcttcgggg tcaaggaact gctgctcgtc gcgttcttcc
     7501 tgtcgatcgg gctgcacggc gtcccggacc tgcagcaggt cctcaccgcc gccctgttgt
     7561 tgctgctcct gccggtgcag acgctggcct acgtcgtgct gctgtcgatg ctgcggatgc
     7621 gccgccgcac ggcggtgctc accgcgctcg tgctggccaa cagctccgag ttcgccctcg
     7681 tcgtcatggc caccgcgcag gatgccggtc tcgtcaccga acgctgggtc acgacgacgg
     7741 ccgtgaccgt ggcgctcagc ttcgtgctct ccagcgcggt caacgccaaa gcggagcaga
     7801 tctcggacgc gatcgagcgc cgctggcccg accctgaccc cgagtcgctc gacgaggcgg
     7861 agcggccgat ccccctgcac gacgtcgatg ccctcgtcct cgggatgggt cgggtcggca
     7921 gcgccgccca ccgccggctg accgagcgcg gtctgcgggt gctcggcatc gagcacgacg
     7981 aggcgcgagt ggcccggctg caggctcagg gcgtcgaggt cgtgttggcg gatgcgaccg
     8041 acagcagcct gtggcgccgc ctggtcgcgg tgagaaccct gcgcacggtg gtgctcgcca
     8101 tgccgttcca cgaggccaac ctcgcgacca tcgaggtcgt gcgggcacgc agcttcaccg
     8161 gcaccgtggc ggccgtcgcc cgctacgacg acgaggtcac cgagctgcac gccaacggcg
     8221 cgaacaccgt gttgcacgtc tacagcggtt caggggtggc cctggccgat gccgctctcg
     8281 acggccccga agaggcgctc caaccctgac ccgcgctgcg tgcggtcagg cggtggtcag
     8341 gccggggcca ccatgtcggc caggtcggcg gcgaggttcg cgtgcacccg ggctcgcacc
     8401 cgcgtcccct cggcctcgtg gcgcgactcc agcacgtccc cctgctcgtg cagccggctc
     8461 acgaggtcgc ccctgtcgta gggcacgagc acgtcgacct cgacgtccgg gtggggaagc
     8521 tcctcgccga tgagggcgcg aagctcctcc atgccctcac cgctgcgggc ggacacgacg
     8581 atcgagtgct tctcgtgccg ctggaggcgc gcgatgacct cggggtcggc gacgtcagcc
     8641 ttgttgatga cgatgacctc gcgcacgtcg gtggcgtcga cgtcggccag cacggcgcgc
     8701 acggccgaga tctggccctc ggggtcgggg tgggagccgt cgacgacgtg cagcagcagg
     8761 tcggcctcgg ccacctcctc cagggtcgag cggaacgcct cgacgagctg gtgcggcagg
     8821 gcccggacga acccgacggt gtcggccagg gtgtagagcc gcccgtcgag cgtctcggcc
     8881 ttgcgcaccg tcgggtcgag cgtcgcgaac agcgcgttct cgacgagcac gccggcaccg
     8941 gtcagccggt tgaggatgct cgacttgccg gcgttggtgt agccggcgat ggccacgctc
     9001 ggcacggcgt tggccttgcg cgacccccgc ttggtgtcac ggacggtctt catctccttg
     9061 atctcacgcc gcagcttggc gatgcgcgtg ttgatccggc gacggtcgag ctcgatcttg
     9121 gtctcaccgg ggcctcgcga gcccatgccc tggcccccgg ccgcctggcc gcccgcctgg
     9181 cgggacatcg actcacccca accgcgcagt cgcggcagga ggtactgcag ctgggcgagc
     9241 tcgacctgcg ccttgccctc ccgggacttc gcgtgctggg cgaagatgtc gaggatcagg
     9301 gccgtgcggt cgatgacctt gacct
//
LOCUS       DAOSOC010000093         9321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7336_length_9321_cov_8.829052, whole genome shotgun sequence.
ACCESSION   DAOSOC010000093 DAOSOC010000000
VERSION     DAOSOC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7336_length_9321_cov_8.829052"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PL014_08190"
     CDS             complement(<1..140)
                     /locus_tag="PL014_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT57107.1"
                     /translation="MKVLSAVAWPYANGPRHLGHIAGFGVPSDVFSRYMRMAGHDVLM
                     VSG"
     gene            270..872
                     /locus_tag="PL014_08195"
     CDS             270..872
                     /locus_tag="PL014_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOT57108.1"
                     /translation="MLAALTGMGLSAAAGLNAYIPFLVVALVSRFTDLITLPAGYEWI
                     DSNWAIGIGSALLLAEIVLDKIPAVDTVNDTIQTFIRPSMGGLIFAASTAASEVDNSA
                     WMQDNPWVSVVLGVIISGLVHTGKMASRPVINAGTAGVGAPVASTIEDGASLGLTVIA
                     LIAPVLVIIALGLLAWLLIWMWLKVRRWRRTRRDPAYQPG"
     gene            complement(869..1615)
                     /locus_tag="PL014_08200"
     CDS             complement(869..1615)
                     /locus_tag="PL014_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57109.1"
                     /translation="MAATATVLVALSVGGCNRDAEGTETASPTGSATASTSTSPTPTP
                     SPSSSLPTVGEVYRQARTAALAAPSGHVVGTQTRDGAPVTVDVEGNANGSNQTVFITT
                     PSGGQAEVITVGADHWLGGDEEFWVEQTGDPAAGSGMVGKYVAISESDATELGSFTLR
                     TMLTETFGLPEIAALETDTSPVEESEVDGKAVYILGKADGARLWVLADGSATLVRAVG
                     PSTAPADLVFSDWGRAQTFTPPPASMVVEG"
     gene            complement(1759..3936)
                     /locus_tag="PL014_08205"
     CDS             complement(1759..3936)
                     /locus_tag="PL014_08205"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HOT57110.1"
                     /translation="MESTRKQATHGGLRLDAGVRRLLEDAFDIDLASVRVHTGTRADA
                     VARSMHADAFAVGPRLHFREGAYDPASEHGLWLLAHEVAHVIQQAHGWVGPSIPRRRL
                     ERQADLAADRVTSGRRAAMGWLEAIPPHRAAGPVTIQCHASWEHRLLGDASAADLGRL
                     AHGSTDRPAALQALRDFLEIWHHDPDHVTPEEIAGRCPGVRTLTLRASGLLVTYGELN
                     TLPDYLSDPTAMDSLPRDILLPILQAVRQEGYANARRLLGNEDPFPIFDGAVAMHLAN
                     GTLNDIWESMWLNDLTANLPQGQPPDSSLSHTDAYSALLARNACHFAPHSWYRWAQSY
                     DLALHKAQDAHRTGDPEAARAAWILHGYADHFLQDSFAAGHLINKTLVMQWFVEWAAP
                     TWFEKVPDWVELQTMTTARQPLISGPGLYDPSDPGVVRDPQTAQEQGSRDARLTMSGV
                     GADAANGIPRDTAYQNYLMFLTSTVAQSAPLALHDDLNKSGLLVASRDQPTAYQVFGD
                     NTMLNGGAGLVIASDTAHLSQQSIRDVLDTGTTTVTRELIQSRFPTVARAASGDLLPL
                     REWNASLKGRAFTAFESVHDIVLGSLQPRMGHVSQDLSHVVVQLSFQGKQGYLGRDAD
                     GWGRLVGRDDATRLRLRMVDGQNYYGTDDDWWLSVGTTGTRAGYVSFYWWNNCGHPDW
                     RYDRVTKRLTSSYSSRAGMSIRDDGYVYCWPSYPAADVELQEV"
     gene            complement(3983..4423)
                     /locus_tag="PL014_08210"
     CDS             complement(3983..4423)
                     /locus_tag="PL014_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57111.1"
                     /translation="MSEQEPQAPAGSFAEHLAQSPSGDLSGGQSNKVELFVQGGSIGW
                     LGEDSGQWAVVTSQDAAVPLKAYVHTDGVLYYCNAGDDSRYLSVSDGYHYVGFYNWVG
                     ARGWTLNGGVLTSLYTNAPLSIYSTGNGYLYANSGYTPVTVQFH"
     gene            4742..5485
                     /locus_tag="PL014_08215"
     CDS             4742..5485
                     /locus_tag="PL014_08215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT57112.1"
                     /translation="MRRQHLTETWLVLGVSLGASAIWSLLRIVDKLTRSAPLGSQTTS
                     MNSSVTPDRPWLDLAYQLTGVALAVVPVLLALHLLRRDDPDEPHTMGFDLRRPGQDLV
                     RGAVLAAVIGLPGLAMYAAAKALDLNTTIAAANLASAWWTVPVLVLAAAQNAVLEEAV
                     MVGYLFKRWGQAGWGRWQIILTSAVIRGTYHLYQGFGGFVGNIVMGVVFGWWYTRSKR
                     VMPLVVAHTILDVVAFVGYALLRDRLSWL"
     assembly_gap    5532..5631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5692..6435)
                     /locus_tag="PL014_08220"
     CDS             complement(5692..6435)
                     /locus_tag="PL014_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOT57113.1"
                     /translation="MNLTLKIWRQAGPRHTGELVVYKVSDISPDMSFLEMLDVLNEQL
                     NAAGQEPIAFDSDCREGICGMCGLMISGEAHGPEKTTTCQLHMRSFRDGEVITIEPWR
                     ANAFPVIKDLVVDRGAFDRIIQAGGYISVNTGSAPDAHAAPVPKPAADRAFTAAECIG
                     CGACVAACPNASAMLFMGAKVTHLSELPQGQPERMARVVSMVDQHDHEGFGGCTNVGA
                     CSQACPKGIPQDVISTLNHDLLAAMRAGH"
     gene            complement(6494..8494)
                     /locus_tag="PL014_08225"
     CDS             complement(6494..8494)
                     /locus_tag="PL014_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOT57114.1"
                     /translation="MTGNTVNAGIAHRLYTEGEKIADTKAPAGPIAEKWTTRKFEAAL
                     VNPANRRKLSVIIVGTGLAGGAAAATLGEAGYHVKSFCFQDSPRRAHSIAAQGGINAA
                     KNYKEDGDSVYRLFYDTVKGGDYRARETNVYRLAEVSAAIIDQCVAQGVPFAREYGGL
                     LDNRSFGGVQVSRTFYARGQTGQQLLIGAYQALERQVAAGTVEQFTRHEMLEVIVSEG
                     RARGIIARDLVTGEIETHLADAVVLASGGYGNVFFLSTNAMGSNVTASWRAHRKGAYM
                     ANPCYTQIHPTCIPVSGDHQSKLTLMSESLRNDGRIWVPKRREDCDKDPRTIAEEDRD
                     YYLERIYPSFGNLVPRDIASRQAKNVCDEGRGVGPMVGDFRRGVYLDFADAISRLGKA
                     AVEAKYGNLFDMYARITGENPYETPMRIYPAVHYTMGGLWVDYDLQSSIPGLFVTGEA
                     NFSDHGANRLGASALMQGLADGYFVLPNTIRDYLAKGPFEPLSTDAPAVVEARTSVEQ
                     RTARLLQIQGERSVDSFHKELGHIMWEYCGMERSEEGLLKAIDLIRGLREEFWRNVRV
                     LGSADTLNQSLEKAGRVADFLELGELMCIDALHRRESCGGHFRAESQTEDGEALRHDD
                     EFLYVAAWEFAGGDGDGEMGAPVLHKEDLVYEAIELKQRSYK"
     gene            complement(8507..9214)
                     /locus_tag="PL014_08230"
     CDS             complement(8507..9214)
                     /locus_tag="PL014_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOT57115.1"
                     /translation="MATTTAPSRPASARRTTIALKTLMAGSGLVFVGYVLLHMYGNLK
                     TLAGEEAFNTYAHHLRTFGEPILPYEGLLWIVRVVLIVAVIGHVYAAFALWSRAGTAR
                     RTKYAAAKTAMRTAWMRWGGVFLILFIVWHLLHFTIMKISVNPDAQGPDIENNPYQLV
                     VASFQVPWMTLIYLLALAALGLHLAHGVFSAQQTLGLTGTPAKYRRAKAIGHAVAALV
                     VVGFAIPPLAILFGLIK"
BASE COUNT         1384 a         3029 c         3286 g         1522 t
ORIGIN      
        1 ccgctcacca tcagcacgtc gtgccccgcc atccgcatgt agcggctgaa gacgtcagag
       61 gggaccccga acccggcgat gtggccgagg tgtcgggggc cgttcgcgta cggccaggca
      121 acagcggaga ggaccttcat ggggaccgat cctaggtgcc cggcgctgca ctcccccacc
      181 gcccatcccg ggactacgct cgaggggtcc tgcgttgcac tgacggcgcg tccgcgtgga
      241 gcggtcgcgg gtgcgaagga aggaagaccg tgctcgctgc cctcaccgga atgggcctgt
      301 ccgccgcggc agggctcaac gcctacatcc cgtttctcgt cgtggccctg gtctcgcgct
      361 tcacggacct catcaccctg cccgcggggt acgagtggat cgacagcaac tgggccatcg
      421 gcatcgggtc cgccctcctg ctcgccgaga tcgtcctgga caagatcccc gccgtcgaca
      481 cggtcaacga cacgatccag acgttcatcc gccccagcat gggtgggttg atcttcgccg
      541 cctccaccgc ggcatccgag gtcgacaact cggcgtggat gcaggacaac ccctgggtct
      601 cggtggtgct cggggtgatc atctcggggc tggtccacac gggcaagatg gcctctcgcc
      661 ccgtgatcaa cgccggcacc gccggagtcg gcgcacccgt cgcgtcgacg atcgaggacg
      721 gcgcctccct cggcctgacg gtcatcgcac tcatcgcccc ggtgctcgtc atcatcgcgc
      781 tgggcctgct cgcctggctg ctgatctgga tgtggctgaa ggtccgtcgc tggcggcgga
      841 cgcggcgcga tcccgcgtac cagcccggct agccctcgac gaccatcgac gccggcggcg
      901 gggtgaaggt ctgggcccgg ccccagtcgc tgaacacgag gtcggccggg gcggtcgacg
      961 gcccgaccgc gcgcacgagg gtggcgctgc cgtcggccag cacccagagt cgggccccgt
     1021 ctgccttgcc gaggatgtac accgccttgc cgtcgacctc gctctcctcc accggggagg
     1081 tgtcggtctc gagagccgcg atctccggca gcccgaaggt ctcggtgagc atggtgcgca
     1141 gggtgaagga gccgagctcg gtggcgtcgg actcgctgat ggcgacgtac ttgccgacca
     1201 tcccggagcc agcggccgga tcaccggtct gctcgaccca gaactcctcg tcgcccccga
     1261 gccagtggtc ggctccgacg gtgatcacct cggcctggcc acccgagggg gtggtgatga
     1321 agaccgtctg gttcgagccg ttggcgttgc cctcgacgtc aacggtgacc ggggccccat
     1381 cgcgggtctg cgtgccgacc acgtgccccg acggggcagc cagggcagcg gtgcgagcct
     1441 gccggtagac ctcaccgacc gtcggcagcg acgacgacgg gctcggggtg ggagtcgggc
     1501 tcgtcgaggt gctggccgtc gcggaccccg tgggggatgc cgtctcggtc ccctcggcgt
     1561 cgcggttgca gcccccgacg gacagggcca ccagcacggt tgcggtcgct gccatggtcc
     1621 gcaggccgga tccggtctcc actgtgctct cctcacgtcg gaaccgccat ggtaggcggc
     1681 cacctccggg tcgacgtgcc gccccaccgc gcgagggtca cgtgtcgtgc ctcctggccg
     1741 gtcggtcttg gtcctgggct acacctcctg cagctcgacg tcggcagcgg ggtaggacgg
     1801 ccagcagtag acgtagccgt cgtcccggat cgacatcccg gcacgtgagg agtacgacga
     1861 cgtgaggcgc ttggtgaccc ggtcgtacct ccagtccggg tgcccgcagt tgttccacca
     1921 gtagaaggag acgtacccgg cccgggttcc cgtggtgccg acggacagcc accagtcgtc
     1981 gtcggtcccg tagtagttct ggccgtcgac catccgcagt cgcaacctcg tggcgtcgtc
     2041 gcggcccacg agccgtcccc agccatcggc atctcgcccc aggtagccct gcttcccctg
     2101 gaaggagagc tggacgacga catgactcag gtcctgcgag acgtggccca tccgcggctg
     2161 gagcgagccg aggacgatgt cgtgaacgct ctcgaaggcg gtgaaggcac ggcccttcag
     2221 cgaggcgttc cactcccgca gcgggagcag gtcacccgac gcggctctcg cgacggtcgg
     2281 gaaccggctc tggatcagct cgcgggtgac cgtcgtcgtg cccgtgtcca ggacgtcccg
     2341 gatggactgt tgcgacaggt gggcggtgtc gctggcgatc accagaccgg caccgccgtt
     2401 cagcatcgtg ttgtcaccga agacctggta cgcggtgggc tggtccctcg aggcgacgag
     2461 cagcccgctc ttgttgaggt cgtcgtgcag cgcgagcggc gcggactggg ccacggtgct
     2521 ggtgaggaac atgaggtagt tctggtaggc ggtgtcacgg gggatgccgt tggcggcatc
     2581 cgcgccgacg ccggacatcg tgagccgagc gtcgcgcgaa ccctgctcct gagcggtctg
     2641 cgggtcgcgc accactccgg ggtccgacgg gtcgtagaga cccggccccg agatcagcgg
     2701 ctgtcgagcc gtcgtcatgg tctgcagctc gacccagtcc ggcaccttct cgaaccaggt
     2761 cggcgccgcc cactcgacga accactgcat gaccagggtc ttgttgatca ggtggcccgc
     2821 ggcgaatgag tcctggagga agtggtcggc gtacccgtgg aggatccagg cggcgcgagc
     2881 agcctctgga tcgccggttc ggtgggcgtc ctgcgccttg tgcagtgcca ggtcgtagga
     2941 ctgcgcccac cggtaccagg aatgcggtgc gaagtggcag gcgttgcgcg cgagcagggc
     3001 cgagtaggcg tccgtgtggc tcagcgagga gtccggcggc tggccctggg gcaggttcgc
     3061 cgtgaggtcg ttgagccaca tcgactccca gatgtcgttc agcgtcccgt tggccaggtg
     3121 catggcgacc gcgccgtcga agatggggaa cgggtcctcg ttgcccagga gcctccgggc
     3181 gttggcatac ccctcctgcc gcaccgcctg caggatgggc aggaggatgt cacggggcag
     3241 gctgtccatc gccgtggggt cggagaggta gtccggcaag gtgttcagct cgccgtaggt
     3301 gacgagcagg ccgctggcgc gcaaggtcag ggtgcgcacc ccggggcagc gcccggcgat
     3361 ctcctccggg gtgacgtggt ccgggtcgtg gtgccagatc tcgaggaagt cgcgaagggc
     3421 ctgcagtgct gccggccggt cggtggagcc gtgcgccagg cggcccaggt cagccgccga
     3481 ggcgtccccc agcaaccggt gctcccacga ggcatgacac tggatggtca cgggtccggc
     3541 ggcacggtgc ggcgggatcg cctcgagcca ccccatcgct gcccgccgcc cggaggtgac
     3601 cctgtccgcg gccaggtcgg cctgccgctc gagccggcgg cgagggatgg acggccccac
     3661 ccacccgtgc gcctgctgga tgacgtgggc gacctcgtgg gcgagcaacc acagcccgtg
     3721 ctcgctcgcc gggtcgtagg ctccctcccg gaagtggagc cggggtccga ccgcgaacgc
     3781 atcggcgtgc atcgacctgg caacggcatc tgcccgagtg ccggtgtgga cccgcaccga
     3841 ggccaggtcg atgtcgaagg cgtcctcgag aagacgtcga acgccggcgt ccagacggag
     3901 tccgccgtgg gttgcctgtt tcctggttga ttccacgacg gaactcctca cgccggcatc
     3961 ggcgatgtgt gggactgggc ggtcagtgga actggacggt caccggcgtg tagccgctgt
     4021 tcgcgtacag atagccgttg ccggtcgagt agatcgacaa cggggcattg gtgtacagcg
     4081 acgtgagcac gccgccgttc agcgtccatc cgcgcgcgcc gacccagttg tagaagccga
     4141 cgtagtggta cccgtcgctg accgacaggt agcgggagtc gtcgccggcg ttgcagtagt
     4201 acaggacgcc gtcggtgtgg acgtaggcct tcaggggcac tgcggcgtcc tgactggtga
     4261 ccacggccca ctgcccgctg tcctcaccga gccatccgat ggacccgccc tggacgaaca
     4321 gctccacctt gttcgactgg ccgcccgaca ggtccccgga gggggattgg gcgaggtgct
     4381 ccgcgaacga gcccgcgggg gcttgtggtt cttgctcgga catgactgtc tccatctgag
     4441 gcgggatgcc aaaacctgtt cacaggcaat tcagagacct tgagaaggtc tgaattgttt
     4501 ggcttcgact attgttggcg agcccagcgg gagcggcaat agtccgttct ggctattgac
     4561 tggcgccaac aattgggcgt tccgaattgc ggcacaattc ctaccattct attggtaaag
     4621 agtgggcatg gaattgatct ggaatctgca tcgcacaagc aaagaattgg tcaattgcag
     4681 ggacaatctc tgccgaggtc ccgacgtggc accgaccggt gcgcggcata cgctgtcctc
     4741 catgcgccgc cagcacctca ccgagacctg gctcgtgctg ggtgtgtccc tgggagcctc
     4801 ggcgatctgg tcactgctgc gcatcgtcga caagctgacc cggtcggcac cgctcgggtc
     4861 gcagaccacc tcgatgaact cgtcggtgac cccggaccgg ccgtggctcg acctcgccta
     4921 ccagctcacc ggggtcgcgc tggccgtggt gccggtgctc ctcgccctgc acctgctgcg
     4981 ccgcgacgac ccggacgaac cccacacgat gggcttcgac ctgcgccggc cgggtcagga
     5041 cctcgtgcgc ggggcggtgc tggccgccgt catcggcctc cccgggctcg cgatgtacgc
     5101 cgccgcgaag gccctcgacc tcaacacgac gatcgccgct gccaacctcg cgagcgcctg
     5161 gtggaccgtc cccgtcctgg tcctcgccgc cgcccagaac gcggtgctcg aagaggcggt
     5221 catggtgggc tacctgttca aacggtgggg ccaggccggg tgggggcgct ggcagatcat
     5281 cctcaccagc gccgtgatcc gaggcaccta ccacctctac caggggttcg gcggcttcgt
     5341 cggcaacatc gtcatggggg tggtcttcgg ctggtggtac acccgctcca agcgggtgat
     5401 gccgctcgtg gtggcccaca cgatcctcga cgtcgtcgcc ttcgtcggct acgcgctgct
     5461 gcgcgaccgc ctcagctggc tctgacccgt cccggaaccg accaacaggt cggtccgggt
     5521 acgccctgac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcgtaccc
     5641 ccagcgacct gttggtcgct gggggtacgc gtttggggtt ggggatgccg gtcagtgacc
     5701 tgcgcgcatc gccgccagga ggtcatggtt gagcgtggag atgacgtcct gcgggatgcc
     5761 cttcgggcag gcctgcgagc aggcaccgac gttggtgcag ccaccaaagc cctcgtggtc
     5821 gtgctggtcg accatggaga cgactcgcgc catccgctcg ggctggccct ggggcagctc
     5881 ggagaggtgg gtgaccttgg cgcccatgaa gagcatggcg ctcgcgttcg ggcaggccgc
     5941 gacgcaggcg ccgcacccga tgcactcggc cgccgtgaac gcccggtcgg cagccggctt
     6001 ggggaccggc gcggcgtggg cgtcgggggc ggacccggtg ttgaccgaga tgtagccgcc
     6061 cgcctggatg atgcggtcga aggcaccgcg gtcgacgacg aggtccttga tgaccgggaa
     6121 cgcgttggcg cgccacggct cgatcgtgat gacctcaccg tcgcggaagc tgcgcatgtg
     6181 cagctgacag gtcgtcgtct tctcgggccc gtgagcctcg ccggagatca tcaggccaca
     6241 catgccgcag atgccctcgc ggcagtcgct gtcgaaggcg atgggctcct gcccggccgc
     6301 gttgagctgt tcgttgagca cgtcgagcat ctcgaggaag ctcatgtcgg ggctgatgtc
     6361 ggagaccttg tagacgacga gctcgccggt gtggcggggg ccggcctggc gccagatctt
     6421 cagggtgaga ttcatgatcg gggtcctcgc gaagtatcga agtgacgaag gaacgtagaa
     6481 cttcgtgggg tggtcacttg tagctccgct gcttcagttc gatcgcttcg tacaccaggt
     6541 cctccttgtg gagcaccggg gcacccatct cgccgtcacc gtcgccaccg gcgaactccc
     6601 aggccgcgac gtacaggaac tcgtcgtcgt gacgcagcgc ctcgccgtcc tccgtctgcg
     6661 actcggcccg gaagtgacca ccgcaggact cgcggcggtg cagggcgtcg atgcacatga
     6721 gctcaccgag ctcgaggaag tcggcgacgc ggccggcctt ctcgaggctc tggttgaggg
     6781 tgtcggccga cccgaggacg cgcacgttgc gccagaactc ctcgcgcaga ccgcggatga
     6841 ggtcgatggc cttgagcagc ccctcctcgg agcgctccat gccgcagtac tcccacatga
     6901 tgtggccgag ttccttgtgg aaggagtcga cactgcgctc gccctggatc tggagcaggc
     6961 gggccgtgcg ctgttcgacc gaggtgcgtg cctcgacgac ggcgggggcg tccgtcgaca
     7021 gcggctcgaa gggccccttg gcgaggtagt cgcggatggt gttcggcagg acgaagtacc
     7081 cgtcggccag gccctgcatg agcgccgagg cacccaggcg gttggcgccg tggtcggaga
     7141 agttggcctc tccggtgacg aacagcccgg ggatcgagga ctggaggtcg tagtcgaccc
     7201 acagaccacc catcgtgtag tggaccgccg ggtagatgcg catcggggtc tcgtacgggt
     7261 tctccccggt gatccgggcg tacatgtcga acaggttgcc gtacttggcc tcgacggctg
     7321 ccttgcccag tcgggagatg gcgtcggcga agtcgaggta caccccgcgg cggaagtccc
     7381 cgaccatagg gccaacaccg cggccctcgt cgcagacgtt cttggcctgg cgcgaggcga
     7441 tgtcgcgcgg cacgaggttg ccgaagctcg ggtagatccg ctccaggtag tagtcgcggt
     7501 cctcctcggc gatggtgcgc gggtccttgt cacagtcctc gcgacgcttg ggcacccaga
     7561 tgcggccgtc gttgcgcagc gactcggaca tcagggtcag cttgctctgg tggtcaccgg
     7621 agaccgggat gcaggtcggg tggatctgcg tgtagcaggg gttggccatg taggcgccct
     7681 tgcggtgcgc ccgccacgac gccgtgacgt tggagcccat cgcgttggtc gacaggaaga
     7741 acacgttgcc gtacccaccg gaggcgagga cgacggcatc cgcgaggtgc gtctcgatct
     7801 cgccggtgac gaggtcgcgg gcgatgatgc cgcgcgcgcg accctcggag acgatgacct
     7861 cgagcatctc gtggcgggtg aactgctcca ccgtgccggc cgcgacctga cgctcgaggg
     7921 cctggtaggc accgatgagc agctgctggc cggtctggcc acgggcgtag aaggtgcgcg
     7981 acacctggac gccaccgaag gaacggttgt cgagcagacc gccgtactcg cgggcgaacg
     8041 gcacaccctg ggcgacgcac tggtcgatga tcgcggcgct gacctcggcc aggcggtaga
     8101 cgttggtctc gcgggcccgg tagtcaccac ccttgaccgt gtcgtagaac aggcggtaga
     8161 cggagtcgcc gtcctccttg tagttcttgg cggcgttgat gccaccctgg gcggcgatcg
     8221 agtgggcccg gcgcgggctg tcctggaagc agaacgactt cacgtggtag ccggcctcgc
     8281 ccaaggtggc cgcggcggcg ccgccggcga gcccggtgcc cacgatgatg accgacagct
     8341 tgcgacggtt ggccgggttg accagggctg cctcgaactt gcgggtggtc cacttctcgg
     8401 cgatggggcc ggcgggcgcc ttggtgtcgg cgatcttctc gccctcggtg tagaggcggt
     8461 gggcgatccc cgcgttcacg gtgtttccgg tcatggtgtc agtgcctcac ttgatcaggc
     8521 cgaagaggat ggcgagcggc gggatggcga acccgacgac gacgagcgct gcgacggcgt
     8581 ggccgatggc cttggcacgc cggtacttcg ccggggtgcc cgtgaggccg agggtctgct
     8641 gcgcactgaa gaccccgtgc gcgaggtgca ggccgagcgc ggcgagagcg agcaggtaga
     8701 tcagggtcat ccacggaacc tggaagctcg cgacgacgag ctggtagggg ttgttctcga
     8761 tgtccgggcc ctgggcgtcg gggttgaccg agatcttcat gatcgtgaag tgcagcaggt
     8821 gccagacgat gaagaggatg aggaagaccc cgccccaccg catccacgcg gtgcgcatcg
     8881 ccgtcttggc ggcggcgtac ttcgtgcgcc gtgcggtgcc ggcccggctc cacagcgcga
     8941 acgccgcgta gacgtggccg atgaccgcca cgatcaacac gacgcgcacg atccacagca
     9001 gcccctcgta gggcaggatc ggctcgccga aggtgcgcag gtgatgggcg taggtgttga
     9061 acgcctcctc ccccgccaag gtcttgaggt tgccgtacat gtgcaaaagg acgtacccga
     9121 cgaacaccag cccggacccc gccatgaggg tcttgagggc gatggtggtg cggcgggccg
     9181 acgccggacg tgagggagct gtcgtagtgg ccacggcagc aacctagcgc ggcgcgccgc
     9241 ccggcggcag gtcaggtgaa ccttggcggg tgtgaggttc gccaattacc cgccggtaga
     9301 catgacgacg cccggcatcc g
//
LOCUS       DAOSOC010000094         9193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7478_length_9193_cov_8.799081, whole genome shotgun sequence.
ACCESSION   DAOSOC010000094 DAOSOC010000000
VERSION     DAOSOC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9193
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7478_length_9193_cov_8.799081"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1039
                     /locus_tag="PL014_08235"
     CDS             158..1039
                     /locus_tag="PL014_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT57116.1"
                     /translation="MTSAADRVPAPLLVLAGIVSVQFGGALAATLVPLIGAGGSVVLR
                     LLFASALLLVFVRPRWRGHSRRAWLTVIAFGVALGLMNFTFYSSLAHLPIGVAVTIEF
                     IGPLSLAAALSRRWLDAVAVAAAAAGVVLISEALSTPFADLEKTGIALALLAGAFWAA
                     YIVLAGRTGGEFPKLEGLALAMVVATVVTLPLGITSAPSWSPDILLKAFGIAVLSSVL
                     PYSLELLALRRLSAKVFGILLSLEPAFAALAGLLVLGQVLSPTQLVGMALVVAASALV
                     LGLGARQRPDPAEAAST"
     gene            complement(1036..>1338)
                     /locus_tag="PL014_08240"
     CDS             complement(1036..>1338)
                     /locus_tag="PL014_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOT57117.1"
                     /translation="ALMGLRQLYFLLGGLLTKLVYLSLGLAVLLAFIGVKLLLHALHE
                     NELPFINGGEHVAVPEIPIWMSLGAIVVILGTTTVASLWKTRKDERERIAGGPTGS"
     assembly_gap    1339..1438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1439..1758)
                     /locus_tag="PL014_08245"
     CDS             complement(<1439..1758)
                     /locus_tag="PL014_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOT57118.1"
                     /translation="MLPAATPSLDLDPWVWLVTIGIAVAVLVFDVIWIARNPHRPSNK
                     ETGIALGIYIGAAILFGLGMWFFAGGQLAGEFFAGWLTEYSLSVDNLFIFLLIMAKFA
                     VPEKL"
     gene            1838..4495
                     /locus_tag="PL014_08250"
     CDS             1838..4495
                     /locus_tag="PL014_08250"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOT57119.1"
                     /translation="MASMLDDLSLLTSLHRQVILESGRGDLLEITDALEARCRSEDPD
                     APGELVNSLDPQTAELIARLLTVHLHLTNLAEERHRARSVRREDGEFGGSAETGDIGP
                     AIADVAASGSDARARIAAMRIHPVLTAHPTEARRRAVASALRRIAAHLDVHDDPESGP
                     SERADSRRRMLDDIDILQRTSTLRITRPTPSDEVKTVLTVFSQSLVHAVPQFQRAVEA
                     ALGGEASDAATLPPVVRFGSWVGGDRDGNPFVTAEVTRETVAAHADQALQILHEAVDR
                     VARMITVDEESTPPTATLRDALANDAASHPRLLAEISKDSPREPHRQKLLVVAARLDA
                     TRREQAGLAYSGPDEALADLLLVQESLVAAGDARAARGELQDVVWMLQTFGFHLAELE
                     VRQHSAVHRAALTELISLLPGEVTDPAAAATDAAFLDDLAINGWPELTAAASATTREV
                     LDTLRVMSWLQQRWGQRSCGRYIVSFSQSASDLVAVRALARLAVGTRPLSLDVVPLFE
                     TGADLDAADTTLERWMGLSSTAAWLEGVDREVEVMLGYSDSAKDVGPASATLALHRAQ
                     QALVEWAARHEVTLTLFHGRGGSLGRGGGPLHRAIAAQPSGSVDGRFKVTEQGEVIFA
                     RYADIRIAEQHLERVASAVLLTDNPTHAAARESARLRYADVAAVVDDASKRAFRALVE
                     QPGFADFFAMSSPLDLLADLRLGSRPSRRSGTESGRSLEDLRAIPWVFAWSMTRVNLP
                     GWYGLGSGLEALGDPTLAREAYREWPVFAALVDVAEMSLAKTDDRLAARFLELGGRPD
                     LAERVLDELARTRREVLSLLDQDEMLARKPHLHTAVRLRRPFVDMLSHLQLRGLSEIR
                     EHGEEEAVGWKRPLLLTINGLAAGLQNTG"
     gene            complement(4502..6613)
                     /gene="uvrB"
                     /locus_tag="PL014_08255"
     CDS             complement(4502..6613)
                     /gene="uvrB"
                     /locus_tag="PL014_08255"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT57120.1"
                     /translation="MRPTTDLQRRVAPFEVVSDFEPAGDQPQAISDLTKRIRAGEQDT
                     VLLGATGTGKSATTAWLIEQVQRPTLVMAPNKTLAAQLANEFRELLPNNAVEYFVSYY
                     DYYQPEAYVPQTDTYIEKDSSINDEVERLRHSATNSLLTRRDVIVVASVSCIYGLGTP
                     QEYVDRMVRLRVGDEVPRDDLLRKFVQMQYTRNDLAFTRGTFRVRGDTVEIIPMYEEL
                     AVRIEFFGDEVEAIYTLHPLTGEVVREESEMYVFPASHYVAGPERMERAIAGIESELE
                     QQLALFERQGKLLEAQRLRMRTTYDIEMMRQVGSCSGIENYSMHIDGRLPGTAPNCLL
                     DYFPEDFLLVIDESHVTVPQIGAMYEGDASRKRTLVDHGFRLPSAMDNRPLKWEEFLE
                     RIGQTVYLSATPGAYELTKATGVVEQIIRPTGLIDPEIILKPTKGQIDDLLHEIRTRA
                     DKDERVLVTTLTKKMAEDLTDYLLDKGVRVRYLHSDIDTLRRVELLRELRLGEYDVLV
                     GINLLREGLDLPEVSLVAILDADKEGFLRSSRSLIQTIGRAARNVSGQVHMYADKVTP
                     SMAEAVEETTRRREKQIAYNLERGIDPQPLRKKIADITDMLGREDADTQELLGSGRAQ
                     SRGKSDGGRGGRGAAAADASLATRRTGPVAASDLAALIQELSTQMHQAAADLHFELAA
                     RLRDEVGDLKKELRQMSEATK"
     gene            complement(6668..7861)
                     /gene="coaE"
                     /locus_tag="PL014_08260"
     CDS             complement(6668..7861)
                     /gene="coaE"
                     /locus_tag="PL014_08260"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT57121.1"
                     /translation="MLRVGLTGGIGSGKSTVSAQLTSLGAVVIDADAVAREVVEPGTP
                     ALGAIADRFGPEVLTSDGVLDRPALGRLVFGDAAALRDLEAITHPAIWARTSELMAAV
                     PPGRVMVHDMPLLVEKQMAADYHLVVVVGVGEAIRVRRLVDLRGMSEHDARARIAAQA
                     SDEQRRAAADVWLDNEGTTEALRAAVLRLWHERIEPFDLNLTHGIRSRLSHPTISAPD
                     PTWPAQAARLLARIRHAVGDVALTLDHIGSTAVPGLVAKDVIDLQVGVASLADADAPE
                     FVTAMAAAGFVRAEGDWHDNGKDGTSWPKRFHGSCDPGRVAHVHVREVGSPGWSWALM
                     FRDWLRADPSERASYACMKTDLAAKAGSIEHYVNGKEPWFDAADQRARTWAESTAWVP
                     PIGRA"
     gene            7966..8217
                     /locus_tag="PL014_08265"
                     /pseudo
     CDS             7966..8217
                     /locus_tag="PL014_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            8260..8433
                     /locus_tag="PL014_08270"
     CDS             8260..8433
                     /locus_tag="PL014_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOT57122.1"
                     /translation="MPPAEQIYVVQPGDTVSALAQKFGLRWVDIAAINKLEDMDLIIV
                     GQKLVIPAQGLAR"
     gene            complement(8524..>9193)
                     /locus_tag="PL014_08275"
     CDS             complement(8524..>9193)
                     /locus_tag="PL014_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT57123.1"
                     /translation="LSLKATQEDPWQHFARTHQIGQIVPGKVTKLVPFGAFVRVEEGI
                     EGLVHISELAERHVELPEQVVTVGTDIFVKVIDIDLERRRISLSLKQANEDGANQVEF
                     DPTLYGMAAEYDEKGDYKYPEGFDAETNEWLEGFEAQREIWEKQYAEAHARWEAHRAQ
                     VEAFAKADAEAAGGSNETSYSSETGSTPAEDGGAPRPPAPAAEGTLASDEALAALREK
                     LTGN"
BASE COUNT         1338 a         3219 c         3112 g         1424 t
ORIGIN      
        1 ccgacggcac cattgcgcgc agcctactcc tgctcacccg cgaccaaccc acaccaggcg
       61 cgctcgccca ggtcctggac tcgatccgga cccgagcacg aggcctcggc agaaacttcc
      121 gcgaaaccga aaccatcgaa acgactagcc tgacgcggtg acgagcgcag cagaccgggt
      181 ccccgcaccc ctcctcgtgc tcgctggcat cgtctcggtg cagttcggcg gggcgttggc
      241 cgccacgctc gtgccgctga tcggcgccgg cgggtccgtc gttctgcgcc tgctcttcgc
      301 cagcgccctg ttgctggtct tcgtccgccc ccgctggcga gggcactcgc gtcgcgcctg
      361 gctcacggtg atcgcgttcg gtgtcgccct cggcctgatg aacttcacct tctacagctc
      421 gctggcccat ctgccgatcg gggtcgcggt gaccatcgag ttcatcggcc ccctcagcct
      481 cgcggcggcg ctatcgcgcc gatggttgga tgccgtcgcg gtcgccgccg cagccgctgg
      541 ggtcgtgctc atctccgagg cactgagcac cccgtttgcc gacctcgaga agaccgggat
      601 cgcgcttgcg ctgctggccg gtgccttctg ggcggcctac atcgtgctcg ccgggcgcac
      661 cgggggtgag ttcccgaagc tcgaggggtt ggcgctcgcc atggtggtgg ccaccgtcgt
      721 cacgctgccg ctcggcatca cgagcgcccc cagctggtcg ccggacattc tcctcaaggc
      781 gttcggcatc gctgtgctct cgtcggtcct gccctactca ctcgagctgc tggcgctgcg
      841 gaggctgagc gcgaaggtct tcggcatcct gctcagcctc gaaccggcct tcgccgcgct
      901 ggcggggctg ctcgtcctcg gccaggtgct ctcgccgacg cagctcgtgg ggatggccct
      961 cgtggtcgcg gcctcggcgc tggttctcgg tctcggcgcg cgccagaggc cggaccccgc
     1021 tgaggcagcc agtacctagc tgccggtggg accgccggcg atgcgctcgc gctcgtcctt
     1081 gcgggtcttc cacaggctgg ccacggtggt ggtcccgagg atgacgacga tcgcgcccag
     1141 cgacatccag atcgggatct ccgggaccgc cacgtgctcg ccgccgttga tgaacggcag
     1201 ctcgttctcg tgcagggcgt gcaggagcag cttcacgccg atgaacgcca gcagcacggc
     1261 cagacccagg ctgaggtaga cgagcttggt gagcaggccg ccgagcagga agtacagctg
     1321 gcgcagaccc atcagggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     1441 cttctccggg acggcgaact tcgccatgat cagcaggaag atgaacaggt tgtcgaccga
     1501 gagcgagtac tccgtcaacc agccggcgaa gaactcgccc gccagctgac caccggcgaa
     1561 gaaccacatc cccagcccga acaggatggc agcaccgatg tagatgccga gcgcgatacc
     1621 cgtctccttg ttggagggcc ggtgcgggtt tcgggcgatc cagatgacgt cgaagacgag
     1681 aaccgcaacg gcgatcccga tcgtcaccag ccacacccac gggtcgaggt cgagggacgg
     1741 ggtggcagcc ggaagcatcg ctgggatcac ttgcgggagt ctaccgagga gcgaacgccc
     1801 tcggcctgct gtgtccgctc gaccctaggc tgcacccatg gccagcatgc tcgacgacct
     1861 gtccctgctc accagcctgc accggcaggt catcctggag tccggccgcg gtgacctgct
     1921 ggagatcacc gacgccctcg aggcgcgctg ccgctccgag gaccccgacg ccccgggcga
     1981 gctcgtcaac agcctcgacc cgcagaccgc cgagctcatc gcgcggctgc tcaccgtgca
     2041 cctgcacctg acgaacctcg cggaggaacg gcaccgcgcc cggtcggtgc gtcgcgagga
     2101 cggcgagttc ggtgggagcg ccgagaccgg cgacatcggc cccgcgatcg cggacgtcgc
     2161 ggcatccggg tcggacgccc gggcacggat cgcggccatg cggatccacc cggtgctcac
     2221 ggctcacccg accgaggccc ggcgccgtgc ggtggcgtcc gccctgcggc gcatcgcagc
     2281 ccacctcgac gtccacgacg accccgagtc gggcccgtcg gagcgtgcgg actcccgccg
     2341 ccgcatgctc gacgacatcg acatcctcca gcgcacctcc accctgcgca tcacccgccc
     2401 caccccctcg gacgaggtca agaccgtgtt gacggtcttc agccagtccc tcgtgcacgc
     2461 ggtcccgcag ttccagcggg ccgtggaggc ggccctgggt ggcgaggcat cggatgccgc
     2521 cacgctgcct ccggtggtcc gcttcggttc ctgggtgggt ggggatcgcg acggaaaccc
     2581 cttcgtgacg gccgaggtga cccgcgagac cgtggccgct cacgccgacc aggccttgca
     2641 gatcctgcac gaggccgtcg accgggttgc ccggatgatc accgtggacg aggagtcgac
     2701 tcccccgacc gcgacgctgc gtgacgccct ggccaacgac gctgcaagcc acccccgctt
     2761 gctggccgag atcagcaagg actcccctcg tgagccgcac cggcagaagc tgctcgtcgt
     2821 ggccgcccgg ctcgacgcga cccgtcgaga gcaggcgggc ctggcgtact ccgggccgga
     2881 cgaggcgctc gcggacctct tgctcgtgca ggagtcgctc gtcgccgctg gtgacgcccg
     2941 ggccgcgcgc ggcgagctgc aggacgtcgt gtggatgctg cagaccttcg gcttccacct
     3001 cgccgagctc gaggtgcgtc agcacagtgc cgtgcaccgg gcggcgctca ccgagctgat
     3061 ctccctgctt cccggtgagg tcaccgaccc ggctgcggcc gccacggacg cggccttcct
     3121 cgacgacctg gcgatcaacg gctggcccga actcactgcc gcagcgagtg cgacgacgcg
     3181 cgaggtgctc gacacgttgc gcgtcatgtc gtggttgcag cagcgctggg gtcagcgcag
     3241 ctgcggccgg tacatcgtct cgttcagcca gagcgccagc gacctcgtcg ccgtgcgagc
     3301 cctcgctcgg ctcgccgtcg gcacacggcc gctctccctc gacgtcgttc cgctgttcga
     3361 gaccggcgcc gacctcgatg ctgccgacac gaccctcgag cggtggatgg gcctgtcgtc
     3421 gacggcggcg tggctcgagg gtgtcgaccg tgaggtcgag gtcatgctcg ggtactcgga
     3481 ctcggccaag gacgtcggcc cggcctcggc gaccttggca ctgcaccgcg cccagcaggc
     3541 cctcgtcgaa tgggcggcgc gccacgaggt gacactgacc ctgttccacg gccgcggagg
     3601 atccctcggt cgtgggggtg gcccgctgca ccgggcgatc gccgcccaac cgtcgggctc
     3661 ggtcgacggc cgattcaagg tcaccgagca gggcgaggtc atcttcgcca ggtacgccga
     3721 catccgcatc gccgagcaac acctcgagcg ggtggcgtca gcggtgctcc tcaccgacaa
     3781 cccgacgcac gccgccgcca gagagtctgc ccggctgcgt tacgccgatg tcgcggccgt
     3841 ggtcgacgac gccagcaagc gggcgttccg ggccctggtg gaacagcccg ggttcgccga
     3901 cttcttcgcc atgtccagcc cgctggacct gctcgcggac ctaagactgg ggtcgcggcc
     3961 gagccgccgc agcggcaccg aaagcggtcg cagcctcgag gacctgaggg cgatcccgtg
     4021 ggtgttcgcc tggtcgatga cccgggtcaa cctcccgggg tggtacggcc tcggcagcgg
     4081 gctcgaggcc ctgggtgacc cgaccctggc gcgagaggcg tatcgcgagt ggccggtctt
     4141 cgccgccctc gtcgacgtcg ccgagatgag cctggccaag accgatgacc ggctggcagc
     4201 gcggttcctc gagctcggag gccgacccga cctcgccgag cgggtgctcg acgaactcgc
     4261 ccgcacccgc cgcgaggtgc tcagcctgct cgaccaggac gagatgctcg cccgcaagcc
     4321 acacctgcac accgccgtcc ggctgcgtcg cccgttcgtc gacatgctca gccacctgca
     4381 gctgcgcggc ctgagcgaga tccgtgagca cggcgaggag gaggcggtcg ggtggaagcg
     4441 gcccctgctg ctcaccatca acggactggc agcagggttg cagaacaccg gctgagcgcg
     4501 cctacttggt ggcctcgctc atctggcgca gctccttctt gaggtcgccg acctcgtctc
     4561 gcagtcgggc ggcgagctcg aagtgcagat cagcggcggc ctggtgcatc tgcgtgctga
     4621 gttcctggat gagggcggcg aggtcactgg cggcgaccgg gccggttcga cgggtagcca
     4681 gcgatgcatc agccgcggcc gccccccgtc cgccacgacc accgtcgctc ttgccgcgcg
     4741 actgtgcccg cccggaaccc aggagctctt gggtgtcggc gtcctcacgg cccagcatgt
     4801 cggtgatgtc ggcgatcttc ttgcgcaacg gctgggggtc gatcccgcgc tcgaggttgt
     4861 aggcgatctg cttctcccgg cgccgggtgg tctcctccac cgcctcggcc atcgacgggg
     4921 tcaccttgtc ggcgtacatg tgcacctggc cggagacgtt gcgagccgcg cgcccgatcg
     4981 tctggatgag gctgcggctg gagcgcagga agccctcctt gtcggcgtcg aggatcgcga
     5041 cgagagagac ctcgggcagg tcgagaccct cgcggagcag gttgatcccg acgaggacgt
     5101 cgtactcccc cagacgcagc tcacgcagca gttccacccg gcgcagggtg tcgatgtcgg
     5161 agtgcaggta tcgaacccgc actcccttgt cgagcaggta gtcggtgagg tcctcggcca
     5221 tcttcttggt cagcgtcgtg acgagcaccc tctcgtcctt gtcggcgcgg gtgcggatct
     5281 cgtgcaggag gtcgtcgatc tggcccttgg tgggcttgag gatgatctcg gggtcgatca
     5341 gacccgtcgg gcggatgatc tgttcgacga caccggtggc cttggtcagc tcgtaggcgc
     5401 ccggggtggc cgacaggtag acggtctgac cgatgcgctc gaggaactcc tcccacttca
     5461 ggggtcggtt gtccatcgcc gagggcagcc ggaagccgtg gtcgaccagg gtgcgcttgc
     5521 gagacgcgtc gccctcgtac atcgcgccga tctgcggcac ggtgacgtgt gactcgtcga
     5581 tgacgagcag gaagtcctcg gggaagtagt cgagcaggca gttcggggcc gtgcccggga
     5641 ggcggccgtc gatgtgcatc gagtagttct cgatccccga gcacgagccg acctggcgca
     5701 tcatctcgat gtcgtacgtc gtgcgcatcc gcaggcgctg ggcctcgagc agcttgccct
     5761 gccgctcgaa gagggcgagc tgctgctcga gctcgctctc gatgccggcg atcgcccgct
     5821 ccatgcgttc cgggccggca acgtagtggg aggcagggaa gacgtacatc tccgactcct
     5881 cgcgcacgac ctcaccggtc agcgggtgca aggtgtagat ggcttcaacc tcgtcgccga
     5941 agaactcgat gcgcaccgcg agctcctcgt acatcgggat gatctcgacg gtgtcacccc
     6001 gaacccggaa ggtgccacgc gtgaacgcca ggtcgttgcg cgtgtactgc atctggacga
     6061 acttgcgcag gaggtcatcg cgaggcacct cgtcaccgac gcgcaaccgg accatccggt
     6121 cgacgtactc ctgcggggtg ccgagcccgt agatgcagga caccgacgcc acgacgatga
     6181 cgtcacgccg ggtcagcagc gagttcgtgg cgctgtgccg aagccgctcg acctcgtcgt
     6241 tgatcgagga gtccttctcg atgtacgtgt cggtctgcgg gacgtacgcc tcgggctggt
     6301 agtagtcgta gtagctgacg aagtactcga cggcgttgtt gggaagcagc tcacggaact
     6361 cgttggccag ctgggcggcc agggtcttgt tcggcgccat gaccagggtc ggccgctgga
     6421 cctgctcgat gagccaggcg gtggtcgccg acttgccggt gcccgtcgca ccgagcagga
     6481 cggtgtcctg ctcccccgcg cggatgcgct tcgtgaggtc ggagatggcc tggggctggt
     6541 cgccggcagg ctcgaagtcg gagaccacct cgaagggcgc cacgcggcgc tgcagatctg
     6601 tcgtcggacg catgtcccta cggtatgccg agccaccgac agccggcccg gttgcgcgtc
     6661 ccgctcctca ggcacgcccg atgggcggca cccaggcggt ggactcggcc cacgtacgag
     6721 cccgctggtc agcggcgtcg aaccagggct ccttgccgtt gacataatgc tcgatcgagc
     6781 ccgccttcgc ggcgaggtcg gtcttcatgc aggcgtacga ggcccgctcg ctcggatcgg
     6841 cccgcagcca gtcacggaac atcagcgccc aggaccagcc gggtgaaccc acctcgcgga
     6901 cgtggacgtg ggcgacccgg ccggggtcgc acgacccgtg gaaccgcttg ggccacgacg
     6961 tgccgtcctt gccgttgtcg tgccagtcgc cctcggcccg gacgaacccc gcggcagcca
     7021 tcgccgtgac gaactcaggt gcgtcggcgt ccgcgagcga ggcgaccccc acctggaggt
     7081 cgatgacgtc cttcgcgacc agacccggca cggccgtgga gccgatgtgg tcgagcgtga
     7141 gcgcgacgtc gccgacggcg tgtcggatgc gcgcgagcag gcgcgccgcc tgggctggcc
     7201 atgtcgggtc cggcgccgag atcgtcgggt gggacaaccg cgatcggatg ccgtgggtca
     7261 ggttgaggtc gaagggctcg atccgctcgt gccacagtcg caggaccgcg gcccgcaacg
     7321 cctcggtggt gccctcgttg tcgagccaga cgtcggccgc ggcacggcgt tgctcgtcag
     7381 aggcctgcgc ggcgatccgg gcccgcgcgt cgtgctccga catgccgcgc aggtcgacga
     7441 gccggcggac ccggatggcc tcccccaccc cgacgacgac gacgaggtgg tagtcggccg
     7501 ccatctgctt ctcgacgagc aggggcatgt cgtgcaccat cacccgcccc ggtggcacgg
     7561 cggccatgag ctcgctcgtg cgggcccaga tcgccgggtg ggtgatggcc tcgaggtcac
     7621 gcagggcagc ggcgtccccg aacacgaggc ggcccagggc cggccggtcg agcaccccgt
     7681 cgctggtcag cacctccggc ccgaagcggt cggcgatggc tcccagcgca ggggtaccgg
     7741 gttcgacgac ctcgcgcgcc acggcatccg catcgatgac gacggcaccc agcgacgtca
     7801 gctgggccga caccgtggac ttgcccgacc cgattccccc ggtgaggccc acgcgcagca
     7861 tgccggtgac cctacaagga gtcgtcacag gtgttccccg attcgtcgtg gcacgacgat
     7921 gcgcgcggct aacctgccag ccatggcaac acacacaccg tcgtctcggg cgacaccctc
     7981 ggacagatcg ccgccaagca caaggtcgcc ctcgcggacc tgatgacgtg gaacggcatc
     8041 aaggacccca acaagatctc cgtggggcag gtgatcctcc tgaccgaccc gaaccccacc
     8101 cacacggtcg tcgccgggga cacgctcgcc aagctcgccg ccacctacga gctgaccgtg
     8161 gacgacctcg tgcgctggaa cgcgatcaag gacgcgaacg tcatcacggt gggtcaggtg
     8221 ctgaagctga gcggacctcc ggccccgcct gctcccccgg tgccgcccgc cgagcagatc
     8281 tacgtcgtcc agcccggcga caccgtgtcc gcactggcgc agaagttcgg cctgcgctgg
     8341 gtggacatcg cggccatcaa caagctcgag gacatggacc tcatcatcgt cggacagaag
     8401 ctcgtgatcc cggcccaggg tctcgcccgc tgaccccaac cgacgaaggc ccggtcaccc
     8461 ctcgcggggg tgaccgggcc ttcgcgcggt gctcacggca tccgatcacg gatgccggtg
     8521 gcctcagtta ccggtgagct tctcgcgcag tgcggcgagc gcctcgtcgg aggcgagggt
     8581 gccctcagca gcgggggccg gcggacgcgg ggcgccaccg tcctcggccg gcgtggaacc
     8641 ggtctcggac gagtaggacg tctcgttgct gccaccagca gcctcggcgt cggccttggc
     8701 gaacgcctcg acctgggccc ggtgggcctc ccagcgagcg tgtgcctcgg cgtactgctt
     8761 ctcccagatc tcacgctgcg cctcgaagcc ctcgagccac tcgttggtct cggcgtcgaa
     8821 gccctcgggg tacttgtagt cacccttctc gtcgtactcg gcggccatgc cgtacagcgt
     8881 cgggtcgaac tcgacctggt tggcgccgtc ctcgttggcc tgcttgagcg acagcgagat
     8941 gcggcgacgc tcgaggtcga tgtcgatgac cttgacgaag atgtcggtac cgacggtgac
     9001 gacctgctcg ggcagctcga cgtggcgctc ggccagctcg gagatgtgca ccaggccctc
     9061 gatgccctcc tcgacgcgga cgaacgcacc gaacggcacc agcttggtga ccttgcccgg
     9121 gacgatctgg ccgatctggt gggtccgggc gaagtgctgc cacgggtcct cctgcgtcgc
     9181 cttgagcgac agg
//
LOCUS       DAOSOC010000095         9104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7587_length_9104_cov_8.989281, whole genome shotgun sequence.
ACCESSION   DAOSOC010000095 DAOSOC010000000
VERSION     DAOSOC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7587_length_9104_cov_8.989281"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1999)
                     /locus_tag="PL014_08280"
     CDS             complement(<1..1999)
                     /locus_tag="PL014_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOT57124.1"
                     /translation="MGLASATGSVARKVGASGRDLEPEHRRDGLGLLLIALAVVVAAR
                     EWWGMTGAVGTVVHAVVAGTFGKIGYAVPIVLLLFGIRMLRAPKDDPATSRAAVGTTA
                     LAFAACGLAHVAAGIPTPPEGADGMRAAGGIIGFLASSPLEAAVSVYGTVPLLLLLGL
                     FGLLVITATPLHQVPERLRETLDRLTGHHRDDTDEVAAPAPRRRRSRADLEADGTLPG
                     DEAFEQAAVVDPVTGKRLRPGQRRPAAAPASAAGSTRPAATTPATAAPRAPVVEKVTL
                     EAPPTTPLPQRVEQLSLAGDITYTLPDNALLEPGSPHKARSAANDAIVEALTTVLDQF
                     EIDAQVTGFSRGPTVTRYEVELGPGIKVERVTALSKNIAYAVASADVRILSPIPGKSA
                     IGIEIPNTDREKVSLGDVLRSQVARTSEHPMVMGVGKDVEGGYVIANLAKMPHLLVAG
                     ATGAGKSSFVNSMITSILMRATPDEVRLVLVDPKRVELTAYEGIPHLITPIITNPKKA
                     AEALQWVVREMDTRYDDLAAFGFKHVDEFNKAVRAGKVTPLPGSERVITTYPYLLVVV
                     DELADLMMVAPRDVEESIVRITQLARAAGIHLVLATQRPSVDVVTGLIKANVPSRMAF
                     ATSSVTDSRVVLDQPGAEKLIGQGDALFLPMGASKPMRVQGA"
     gene            complement(2264..3949)
                     /locus_tag="PL014_08285"
     CDS             complement(2264..3949)
                     /locus_tag="PL014_08285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOT57125.1"
                     /translation="MSHPHPELQAPGPLAPGGVRVVPLGGLGEIGRNMTVVEHRDQLL
                     VIDCGVLFPDEHHPGIDLILPDFEYLEGRLADVQALVLTHGHEDHIGAVPYLLRLRPD
                     LPVIGSTLTLALVEAKLKEHRITPVLRVVQEGERLQFGEFDCEFVAVNHSIPDALAVA
                     VRTSGGTLLHTGDFKMDQLPLDGRLTDLRAFARLGEEGVDLFLTDSTNAEVPGFTTPE
                     RDISSAIDKVFRHAERRIIVACFSSHVHRVQQVLDAAEKAGRKVAMVGRSMLRNMAIA
                     ADLGYLRVPDGILVDVKSLADLPDDEVVLVCTGSQGEPMAALSRMANRDHRIDVGPGD
                     TVLLASSLIPGNENAVYRVINGLMRLGANVVHKGNANVHVSGHASAGELLYCYNIVRP
                     RNVMPVHGEWRHLVANAELAVATGVPRDRVVVAEDGVVVDLVDGVASIAGAVPCGYVY
                     VDGASVGEADDALLKDRRILGDEGFVSVIVVIDASTGAVVAGPEVTARGFIEAQARFD
                     EVVPQLVEALARATADGITDTYQLQQVIRRCVGGWMGRTIRRRPMIIPVVIES"
     gene            complement(3946..>4830)
                     /gene="dapA"
                     /locus_tag="PL014_08290"
     CDS             complement(3946..>4830)
                     /gene="dapA"
                     /locus_tag="PL014_08290"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT57126.1"
                     /translation="PFGRVLTAMVTPMTADGEVDPAATDALVDHLLATGHDGIVVNGT
                     TGEASTLTDDESVEMMRRVKERAGDRAAVVAGVGSNDTRHAVHMARRAADVGADALLL
                     VSPYYNKPTQPGLIAHCHAVADSTDLPVVLYDIPGRTGIPFTTPTLIDLAEHPRIVAV
                     KDAKGDLAASTKVMAATDLLWFSGEDALTLPLLAIGAVGTVSVVGHVAGAQYAAMIAA
                     VDRGDLAEARRIHEALIPIVDAIMTPSQGAIMAKAALVELGVIASATVRLPLVESPVE
                     HLEALRAALGTLPGILDR"
     assembly_gap    4831..4930
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4933..5742
                     /locus_tag="PL014_08295"
     CDS             4933..5742
                     /locus_tag="PL014_08295"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOT57127.1"
                     /translation="MRQYLDLIDHVMTHGVDKGDRTGTGTRSVFGYQMRFDLAEGFPV
                     VTTKRLHLRSIIGELVWFLRGDTNVRWLQERGISIWDEWADENGDLGPVYGHQWRSWP
                     TPDGRSVDQIATVIESIRTSPDSRRHIVSAWNVADVDDMALPPCHTMFQFYVRPAADG
                     GPAHLDCQLYQRSADIFLGVPFNIASYALLTMMVAQQTDLRPGHFIHTLGDAHLYSNH
                     LDQARLQLSREPFPLPMMHITRRGGIDDYDLEDFELEGYQAHPSIKAPIAV"
     gene            5753..6220
                     /locus_tag="PL014_08300"
     CDS             5753..6220
                     /locus_tag="PL014_08300"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOT57128.1"
                     /translation="MTRLTIIAAVARNGVIGHRGGMPWHLPGELAQFKATTLGHTLVM
                     GRATFDSIGRALPGRRTIVITRNPTWHHAGVETAHSFADALALAGPGDEVFAAGGAQI
                     YELALPFAQRLVITEVDAEPEGDTWFPSWARADWVEVSREVHDGWSRVVHERV"
     assembly_gap    6330..6429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6430..6737)
                     /locus_tag="PL014_08305"
     CDS             complement(<6430..6737)
                     /locus_tag="PL014_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOT57129.1"
                     /translation="MLPPLDPGSGPTTTVTSTGGRPLGLENLPDGRILVCDAHRGLLA
                     LDPASGDLETLLTEVDGDRLLFTNNAAVAPDGTIWFSDSSRIWPIEEWKNDLIEHTCT
                     G"
     gene            complement(6821..7249)
                     /locus_tag="PL014_08310"
     CDS             complement(6821..7249)
                     /locus_tag="PL014_08310"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT57130.1"
                     /translation="MATTRTASAHWEGSLMEGAGQVSLESSGIGTYPVSWPSRAEAAN
                     GQTSPEELLAAAHSTCFSMALSHGLAQAGTPPTTIDTTADVTFQPGTGITGIVLKVVG
                     DVPGITAEQFQTAAEAAKAGCPVSQALAAVPITLEATFAG"
     gene            7374..8105
                     /locus_tag="PL014_08315"
     CDS             7374..8105
                     /locus_tag="PL014_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT57131.1"
                     /translation="MTIHLERGEQIATITIDRTHRRNALDLASLEDLHAAVVRCVADG
                     TRAIVLTGADGHFCAGADLTELEDVSFTTRLAQVLAHLAAVPVTTVAAIEGSCMGLGM
                     QLALACDVRVVGASARFAVPVARLGLMVDHWTLTRLARLWGEGTARHLVLTGAVLDAD
                     DAWRLGFASQQGSLADAQALAARAARLAPLTQAGSKLGFDAQMHDPEAVGRYEAAFAR
                     AWASDDLTEGRRAFAERREPIFRGR"
     gene            8111..8782
                     /locus_tag="PL014_08320"
     CDS             8111..8782
                     /locus_tag="PL014_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT57132.1"
                     /translation="MSETHEHHHGGHEHGGHGHDEHGHDGHGHPSAAGAGPLADASVR
                     RARVNDAPAVGLVQAVVFREAYSGRVPDEVVAQFEPDAFARSWRESLTAPPGGIHRLL
                     VACAGDQVVGLCAIGPSQDPDAGGATGEVTVLGVHPDARRQGHGSRLLNAGADLLREA
                     GADTVSLWLLAHDESTREFAAASGFAPDGAYRDRVVSPDGGTLREVRLSASLVDAEPG
                     DPGPA"
     gene            8779..>9104
                     /locus_tag="PL014_08325"
     CDS             8779..>9104
                     /locus_tag="PL014_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOT57133.1"
                     /translation="MSLDEPLSPTRRREVVRQAWSVGVASGTYGISFGALAVAAGLDI
                     WQAQVLSLVMFTGGSQFAFVGIIAAGGLAAAPAAIATATMLGIRNGLYGLQTSQLLRV
                     TGVRRLA"
BASE COUNT         1223 a         3158 c         3238 g         1285 t
ORIGIN      
        1 aggcaccctg cacgcgcatc ggcttgctcg cacccatcgg caggaagagc gcgtccccct
       61 gaccgatgag cttctcggct cccggctggt cgagcacgac gcgcgagtcg gtgacgctgg
      121 aggtcgcgaa cgccatccgc gaggggacgt tggccttgat gaggccggtg acgacgtcga
      181 cgctcggccg ctgggtcgcc aggacgaggt ggatgccggc cgcacgggcg agctgggtga
      241 tgcggacgat cgactcctcg acgtcacgcg gggcgaccat catcaggtcc gccagctcgt
      301 cgacgacgac gaggaggtag gggtaggtcg tgatgacccg ctcggaaccc ggcagcgggg
      361 tgaccttgcc ggccctgacg gccttgttga actcgtcgac gtgcttgaag ccgaacgcgg
      421 cgaggtcgtc gtagcgggtg tccatctccc ggaccaccca ctgcagggcc tcggcggcct
      481 tcttcgggtt ggtgatgatc ggggtgatga ggtgcgggat gccctcgtac gccgtcagct
      541 cgacccgctt ggggtccacg agcacgagac gcacctcatc gggggtggcc cgcatgagga
      601 tcgaggtgat catcgagttg acgaagctcg acttgcccgc gccggtggcg ccggcgacga
      661 gcaggtgggg catcttcgcg aggttggcga tgacgtagcc gccctcgacg tccttgccga
      721 cgcccatcac catcgggtgc tcgctggtgc gcgccacctg gctgcgcagc acgtcgccga
      781 ggctgacctt ctcgcggtcg gtgttcggga tctcgatgcc gatcgcggac ttgccgggga
      841 tcgggctgag gatgcgcacg tcggccgagg cgacggcata cgcgatgttc ttggagaggg
      901 cggtgacccg ctcgaccttg atgccggggc cgagctcgac ctcgtagcgg gtgaccgtcg
      961 ggccacggct gaagccggtg acctgggcgt cgatctcgaa ctggtcgagc accgtcgtca
     1021 gcgcctcgac gatggcgtcg ttggctgcgc tgcgtgcctt gtgcgggctg ccgggctcga
     1081 ggagcgcgtt gtcgggcagg gtgtaggtga tgtcgccggc gagggagagc tgctccaccc
     1141 gctggggcag cggtgtcgtc ggcggcgcct cgagggtgac cttctccacg acaggggcgc
     1201 gaggggctgc cgtggccggc gtcgtcgcgg ccggccgggt cgagccggcg gcggaggcag
     1261 gtgcggccgc gggccggcgc tgcccggggc gcaggcgctt gccggtgacg gggtcgacga
     1321 cggctgcctg ctcgaacgcc tcgtcacccg gcagggtgcc gtcggcttcg aggtcggcgc
     1381 ggctgcggcg ccgccgtggt gccggggcag cgacctcgtc ggtgtcgtcg cggtggtggc
     1441 cggtgagccg gtcgagggtc tcgcggaggc gctcgggcac ctggtgcagc ggggtggcgg
     1501 tgatgacgag cagcccgaac aggcccagga gcagcagcag gggaacggtg ccgtagacgg
     1561 agacggctgc ctcgagcggg ctggaggcga ggaagccgat gatgccgccg gcggcccgca
     1621 tgccgtcagc gccctcgggc ggcgtcggga tgccggcggc gacgtgggcg agcccgcatg
     1681 ccgcgaaggc cagcgccgtg gtgccgacgg ccgcacggga ggtcgccggg tcgtccttgg
     1741 gtgcgcgcag catccggatg ccgaacagca ggagcacgat cggcacggca tagccgatct
     1801 tgccgaacgt cccggccacg acggcgtgca cgacggtgcc cacggcgccg gtcatccccc
     1861 accactcgcg ggccgcgacg accacggcga gcgcgatgag cagcaggcca aggccgtcgc
     1921 gacggtgctc gggctcgagg tcgcggcccg aggcaccgac cttgcgggcg accgatccgg
     1981 tggcgctcgc cagccccatc cacgtggcct gcacggcgcg caggggcagg ggcaggccac
     2041 ccgagcgggc ggttccgctt cccttgcggg agcggggcgc cggccggcgg gttgcggggc
     2101 gcgacgccgg agttcggccg cgcgcggagg aggacgtacg agtcgccatg gtcgcaggct
     2161 acgcgacggt cacacgagac acacgtgtca cacgcggcac tcccacgaca ttcacgcgcg
     2221 tccacggacc gccctcgtgg tcgacctgtg ggcgcggtga gactcaggac tcgatgacga
     2281 cggggatgat catcgggcga cgccggatcg tgcggcccat ccagcccccg acgcaccgcc
     2341 ggatcacctg ctgcagctgg taggtgtcgg tgattccgtc cgccgtggcc ctggccagcg
     2401 cctcgacgag ctggggcacg acctcgtcga agcgcgcctg cgcctcgatg aacccccggg
     2461 ccgtgacctc cggacctgcg acgaccgcac ccgtgctcgc gtcgatgacg acgatgaccg
     2521 agacgaatcc ctcgtccccg aggatgcggc ggtccttcag cagggcgtcg tcggcctcac
     2581 cgaccgaggc cccgtcgacg tagacgtacc cgcaggggac cgccccggcg atcgaggcga
     2641 ccccgtcgac gaggtccacg acgacgccgt cctccgcgac gaccacccga tcccgcggca
     2701 ccccggtggc gaccgcgagc tcggcgttgg cgacgaggtg ccgccactcg ccgtgcaccg
     2761 gcatcacgtt gcgcgggcgc acgatgttgt agcagtagag cagctccccc gcgctggcgt
     2821 ggccagagac gtggacgttg gcgttgccct tgtgcacgac gttggcgccg aggcgcatca
     2881 gaccgttgat cacccggtag acggcgttct cgttgcccgg gatgagcgag gaggccagca
     2941 gcacggtgtc accggggccg acgtcgatgc ggtggtcgcg gttggccatc cgggacaggg
     3001 cggccatcgg ctccccctgg ctgccggtgc agacgagcac cacttcgtcg tccggaaggt
     3061 ccgcgagcga cttgacgtcc acgaggatgc cgtcgggcac tcgcaggtag cccaggtcgg
     3121 cggcgatcgc catgttgcgc agcatcgatc ggccgaccat ggcgaccttg cgaccggcct
     3181 tctcggcggc gtcgagcacc tgctgcaccc ggtgcacgtg cgaggagaag cacgcgacga
     3241 tgatccgccg ctcggcgtgc ctgaacacct tgtcgatcgc ggaggagatg tcccgctccg
     3301 gggtggtgaa cccgggcacc tcggcattcg tggagtcggt gaggaagagg tcgaccccct
     3361 cctcccccaa ccgggcgaac gcgcgcaggt cggtcagccg accgtccagc ggcagctggt
     3421 ccatcttgaa gtccccggtg tgcagcagcg tccccccgct ggtgcggacc gccacggcga
     3481 gggcgtcggg gatggagtgg ttgacggcga cgaactcgca gtcgaactcg ccgaactgga
     3541 ggcgctcccc ctcctgcacc acgcgcagca ccggcgtgat gcggtgttcc ttgagcttgg
     3601 cctcgacgag ggccagggtc agcgtcgagc cgatgaccgg aaggtccggg cgcagccgca
     3661 gcaggtaggg caccgcaccg atgtggtcct cgtgaccgtg ggtgagcacc aaggcctgca
     3721 cgtccgcgag gcgaccctcg aggtactcga agtcggggag gatcaggtcg atgccggggt
     3781 ggtgctcgtc ggggaagagc accccgcagt cgatgacgag gagctggtcg cggtgctcga
     3841 ccacggtcat gttccgcccg atctcgccga gcccaccgag cgggacgacc cgcacgccac
     3901 cgggcgcgag cggccccgga gcctgcagct cggggtgcgg gtggctcatc gatccaggat
     3961 gccggggagc gtccccaggg cggcgcgcag cgcctcgagg tgctcgacgg ggctctcgac
     4021 gaggggcagt cgaacggtcg ccgacgcgat gacgccgagc tccaccagcg cggccttggc
     4081 catgatggcc ccctggctcg gcgtcatgat ggcatcgacg atcgggatga gagcctcgtg
     4141 gatgcggcgc gcctcggcca ggtcgccacg gtccacggcg gcgatcatcg cggcgtactg
     4201 cgcaccggcg acgtgaccga cgaccgagac ggtgccgacg gcaccgatgg ccagcagcgg
     4261 cagggtcagg gcgtcctcgc cgctgaacca cagcaggtcg gtggcggcca tgaccttcgt
     4321 ggatgccgcg aggtcaccct tggcgtcctt gacggcgacg atgcgcgggt gctcggccag
     4381 gtcgatgagc gtcggtgtgg tgaacgggat gccggtgcgc cccgggatgt cgtagagcac
     4441 gaccggcagg tcggtgctgt ccgccaccgc gtggcagtgc gcgatcagcc cgggctgggt
     4501 gggcttgttg tagtaggggc tgacgagcaa cagcgcgtcg gcaccgacgt ccgcggcacg
     4561 acgggccatg tgcaccgcgt gccgggtgtc gttggagccg acgcccgcca cgacggccgc
     4621 ccggtccccc gcgcgctcct tgacgcggcg catcatctcg acgctctcgt cgtcggtgag
     4681 cgtggaggcc tccccggtcg tgccgttgac gacgatgccg tcgtggccgg tggccaggag
     4741 gtggtcgacg agggcgtcgg tggccgccgg gtcgacctca ccgtccgccg tcatcggggt
     4801 gaccatcgcg gtcaggacac ggccgaaggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn ccgtgcgcca gtacctcgac ctcatcgacc acgtgatgac ccacggggtc
     4981 gacaagggtg accgcacggg caccggcacc cgcagcgtct tcggctacca gatgcgcttc
     5041 gacctggccg agggcttccc ggtcgtcacg accaagcggc tgcacctgcg ctcgatcatc
     5101 ggcgagctcg tgtggttcct gcgcggcgac accaacgtgc gctggctcca ggagcgcggc
     5161 atctcgatct gggacgagtg ggccgacgag aacggcgacc tcgggccggt ctacggccac
     5221 cagtggcggt cctggcccac gcccgacgga cggtccgtcg accagatcgc caccgtcatc
     5281 gagtcgatcc gcacctcgcc cgactcgcgc cgccacatcg tgtcggcctg gaacgtcgcc
     5341 gacgtcgacg acatggccct gcccccgtgc cacacgatgt tccagttcta cgtcaggcct
     5401 gccgccgacg ggggcccggc ccacctggac tgccagctct accagcgatc ggccgacatc
     5461 ttcctcggcg tcccgttcaa catcgcgtcc tacgccctgc tgacgatgat ggtcgcccag
     5521 cagaccgacc tgcgccccgg acacttcatc cacaccctgg gcgacgccca cctgtactcc
     5581 aaccacctcg accaggcccg cctccagctc agccgcgagc cgttccccct cccgatgatg
     5641 cacatcaccc gcaggggcgg catcgacgac tacgacctcg aggacttcga gctcgagggc
     5701 tatcaggccc atccgtcgat caaggcgccc atcgccgtct gaggagcacc cgatgacccg
     5761 cctcacgatc atcgccgctg tcgcccgcaa cggcgtcatc gggcaccgcg gggggatgcc
     5821 gtggcacctg cccggcgaac tggcccagtt caaggcgacg accctcggcc acaccctggt
     5881 gatggggcgt gcgacgttcg actccatcgg tcgcgccctg cccggccgcc gcaccatcgt
     5941 gatcacccgc aacccgacct ggcaccacgc cggggtcgag acggcgcact cgttcgccga
     6001 tgcgctcgca ctggcggggc ccggcgacga ggtgttcgcc gcgggcggag cccagatcta
     6061 cgagctcgcc ctccccttcg cccagcggct ggtcatcacc gaggtcgacg ccgagcccga
     6121 gggtgacacg tggtttccct catgggcccg tgccgactgg gtcgaggtca gccgcgaggt
     6181 gcacgacggc tggtcacgag tggtccacga gcgcgtctga ccacgcgatc gcgggttcga
     6241 cgaggctgcc gagccagacc cggccgtcgt gctcgcgcac ccctgtcgcc aggtgccagt
     6301 gcgtggcgtc gaaggccagg tcgtgcaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnc cggtgcacgt gtgctcgatg aggtcgttct tccactcctc gatcggccag
     6481 atccggctgg agtccgagaa ccagatcgtg ccgtcgggcg ccacggcggc gttgttcgtg
     6541 aagagcaacc ggtcaccgtc gacctccgtg agcagggtct cgaggtcgcc gctggcgggg
     6601 tcgagggcga gcagccctcg gtgtgcgtca cagacgagga ttcgcccgtc gggcaggttc
     6661 tccagcccga ggggtcgccc gcccgtggag gtgaccgtcg tcgtcggtcc ggagccgggg
     6721 tcgagaggag gcaacacgca cgagatcgag ccctgggccc tgcgtgttgc ctcctctcga
     6781 tggggtggag ccgaccgggt ggagccgccg gcgcagcggg tcagcccgcg aaggttgcct
     6841 cgagcgtgat cggcaccgca gccagggcct ggctgacggg gcagccggcc ttggcggcct
     6901 cggcggccgt ctggaactgc tcggcggtga tgccgggcac gtcaccgacg accttgagca
     6961 cgatgccggt gatgccggtg ccgggctgga acgtcacgtc ggccgtggtg tcgatcgtcg
     7021 tgggcggcgt accggcctgg gcgagcccgt gcgagagcgc catcgagaag cacgtcgagt
     7081 gcgccgccgc cagcagttct tccgggctgg tctggccgtt cgcggcctcg gcgcgcgacg
     7141 gccaggacac ggggtaggtc ccgatgccgg aggactccag agagacctgc ccggcccctt
     7201 ccatgagtga gccctcccag tgggccgatg cggtgcgagt cgtcgccatc agtgttctcc
     7261 tcgtgtcgtt gcgtggtggg tggtgcctgc cgcgggtgca acacacccgg cccgaagatc
     7321 catccacccg ggtgccgcga gtctgccagt ccccggctgg gtagggtcgg gcggtgacca
     7381 tccatctcga gcgcggtgaa cagatcgcca ccatcaccat cgatcgcacc caccggcgca
     7441 acgccctgga cctcgcctcg ctcgaggacc tgcacgcggc cgtcgtccgc tgcgtcgccg
     7501 acggcacgcg cgccatcgtg ctcaccggtg ctgatgggca cttctgcgcc ggggccgacc
     7561 tcaccgagct cgaggacgtc tcgttcacca cccgcctggc ccaggtgctc gcccacctgg
     7621 ccgcggtgcc cgtgacgacc gtggccgcga tcgagggctc gtgcatgggt ctcgggatgc
     7681 agctcgcgct cgcctgcgac gtgcgcgtcg tcggtgccag cgctcgcttc gccgtgccgg
     7741 tcgcccggct ggggctcatg gtcgaccact ggaccctcac ccgcctggcc cggctctggg
     7801 gtgagggcac cgcccgccac ctcgtgctga ccggcgccgt cctggatgcc gacgacgcct
     7861 ggcgtctggg ctttgcctcc cagcagggat cactggccga cgcccaggcc ctcgccgctc
     7921 gtgccgcccg cctcgccccg ctgacccagg ccggctccaa gctcgggttc gacgcgcaga
     7981 tgcacgaccc cgaggcggtc gggcgctacg aggcggcctt cgccagggcc tgggccagtg
     8041 acgacctcac cgagggccgc cgagccttcg ccgagcgccg cgaacccatc ttccgaggac
     8101 ggtgatcacg gtgtccgaaa cccacgagca ccaccacggc ggtcatgaac acggtgggca
     8161 cggccacgac gagcacggcc acgacggaca cggccacccg tccgccgccg gtgccggccc
     8221 tctcgccgac gccagtgtcc gccgcgccag ggtcaacgac gcccccgcag taggcctcgt
     8281 gcaggcggtg gtgttccgcg aggcctactc cgggcgcgtc ccggacgagg tcgtcgccca
     8341 gttcgagccc gatgcgttcg cgcgctcgtg gcgggagagc ctcacggcgc caccgggcgg
     8401 catccaccgc ctgctcgtcg cctgcgccgg tgaccaggtc gtcggcctgt gcgcgatcgg
     8461 gccctcgcag gacccggatg ccggcggcgc caccggtgag gtcaccgtcc tcggcgtgca
     8521 tcctgacgcc cggcgccagg gccatggctc acgcctgctc aacgccggcg ccgacctgct
     8581 gcgggaggcc ggggcggaca cggtgagcct gtggctgctc gcccacgacg agagcacccg
     8641 cgagttcgcc gccgcatccg gcttcgcccc cgacggcgcc taccgcgacc gcgtcgtctc
     8701 ccccgacggc ggcaccctcc gggaggtgcg cctgtcggcg agcctggtcg acgccgagcc
     8761 gggcgacccc gggccggcgt gagcctcgac gagcccctgt cccccacgcg gcgtcgggag
     8821 gtcgtgcgtc aggcgtggtc ggtcggcgtc gccagcggca cgtacggcat cagcttcggg
     8881 gccctggccg tggcggccgg gctggacatc tggcaggcgc aggtgctctc gctcgtgatg
     8941 ttcacgggcg gctcgcagtt cgccttcgtc gggatcatcg ccgccggtgg cctggccgcc
     9001 gcgcccgccg ccatcgcgac cgcgaccatg ctcggcatcc gcaacgggct ctacgggttg
     9061 cagacctcgc agctgctccg ggtcaccggg gtcaggcgcc tggc
//
LOCUS       DAOSOC010000096         9099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7594_length_9099_cov_6.482862, whole genome shotgun sequence.
ACCESSION   DAOSOC010000096 DAOSOC010000000
VERSION     DAOSOC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7594_length_9099_cov_6.482862"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1937)
                     /gene="menD"
                     /locus_tag="PL014_08330"
     CDS             complement(132..1937)
                     /gene="menD"
                     /locus_tag="PL014_08330"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HOT57134.1"
                     /translation="MNPSQALSTVVVDELVRGGVREVVLCPGSRSAPLAYALVAAERA
                     GHLRLHVRVDERSAGFLALGLARGSRMPVAIVTTSGTAVANLHPAVLEAHHAGVPLVV
                     VSADRPHELRGTGANQTTAQPGIFGGSTRWSADLPAPVERPGLGPFWRSTVCRALAAA
                     AGHIGGEPGPVHLNVALREPLVPDLAPDVTPDLADEAVGGSASGQTNEPTPDPTADWP
                     DNLDGRADRAPWVRTGVTEVTNWSHPSHPVTPLDEVARTLVVVGDTRDPALGERAAAW
                     ATERGHPIVAEPFGTAALRAEALPHGPLLLTVTDWLDAHAPDRVVVVGRVTLSRAVGA
                     LVRRPGLVVEVVTEGSQWPDPSHVAAAVHPPAALRLGGPTTPDDDHGWAQQWRSAAAR
                     LADAVAAQPFPWPSGLAVASAVVDGLPGDGVLVLGPSNPVRDLDLAIAGSITDEVLAN
                     RGLAGIDGVVSTAVGVALARDLVPTVALVGDLTFLHDTNGLLIGPGEQVPDLTLVVVN
                     DDGGGIFTTLEPGAPERAADFERVFGTPTATDLGALCQAHGIRHEVVATSADLTDAVA
                     QPHHGIRVVEVRVDRATHRAAHTDLRTLAAAALTP"
     gene            complement(2008..3177)
                     /locus_tag="PL014_08335"
     CDS             complement(2008..3177)
                     /locus_tag="PL014_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57135.1"
                     /translation="MRVDVRSETLTAAAQRVLAIADELREDAGRLAAVVAGVGPPRVR
                     SPGEAVLVARWLVLEGRALALIGPVGLWGDALELDGLAAALRSAARMYVEVERGVAGV
                     LSAVAAGADVAGRVGWFVDGPVNGSDPIVRAVPSTLTGPLVAHGGVTGRVLGVADLVA
                     AGEGLDGGRVRVLETTRGDGGSAWVVIVPGTQEWSPRPGANPFDLTTDVRALTGDVTI
                     AAAGVSAALARSRAGSGRSSPQDPVMLVGHSQGGILAAALASDPAFRTGNRVTHVVTS
                     GAPVALFPVPPTVKVLSIEHADDPVPGLDLTPNPGGQSWTTIVAPGDGRGAPLDPDRH
                     RLSTYVQTVRAAEGAPRGAVPGLDVWQVGAGDVLGRQVRSVHDHVIERAGATMPP"
     gene            complement(3174..3455)
                     /locus_tag="PL014_08340"
     CDS             complement(3174..3455)
                     /locus_tag="PL014_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57136.1"
                     /translation="MGKDPVEELQRLSREAGAAADRLRERRAAVSRFALVWWQGGAAQ
                     TYRGAVERRVAGLAATEAELLFVAQACHELAGLFAAESAARDLGSGLAS"
     gene            3615..5009
                     /locus_tag="PL014_08345"
     CDS             3615..5009
                     /locus_tag="PL014_08345"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HOT57137.1"
                     /translation="MPHHDLVIIGSGSGNSLLTPELDGLDVAIVEGGTFGGTCLNVGC
                     IPTKMLVLPADRIVDAADAARLGVTFAQPTVDWPGIRDRIFGRLDAISAGGRAYRDLQ
                     AHTTVYAAHARFTGPRQLALSTGEVLTADRVVVAAGAHAVGLPVEGLRSPDPARGLHT
                     SDTVMRLDALPPRMVVIGGGFVACELAHVFSSLGVQVTQVQRGPSLLRHHEPAIAQRY
                     TQVAAHHYDVRLSATPTSAHRTDGVWRIDLDTASGPAHVEAEVVLLAVGRRPSTDGLD
                     PAAGGLEVHADGRLAVDAQLRTTADAVWALGDVCSPHQLKHVANHEARIVAHNLAVDL
                     GRRAGPVQRTDDRPVPHAVFGHPQVAAFGPTSADLDAAGTTYVSATQAFGDTAYGWAL
                     EDTTGLLTIHASPEGRILSAHAVGPHSSTLIQPLIQAASFGQHAHEVARGQYWIHPAL
                     AEVVENALLGLPLG"
     gene            complement(5038..5886)
                     /gene="heR"
                     /locus_tag="PL014_08350"
     CDS             complement(5038..5886)
                     /gene="heR"
                     /locus_tag="PL014_08350"
                     /inference="COORDINATES: protein motif:HMM:NF037099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HOT57138.1"
                     /translation="MSTFPLEISTPSVAVPPERARSLRRFNVGMGLLHLVSGAAMVAL
                     STDFTLPVSVFSLGGPPGTPLEAGTLSGVLDVPLAWATASFLFLSAFFHFVIASPWGF
                     PRYVGELERGRNRFRWVEYSLSSTLMIVLIGLVTGITDLAALIGIAFANIAMILFGWL
                     MEVSNNGLMHGRDQSPARGERAWWTPFWFGCVAGIGPWLAIGAYLWASIAVLDGQGPP
                     GFVWGILISLFVLFNTFALNQWLQYRQVGRWRDYLFGEWQYVILSLVAKSVLAWQIFA
                     NTLIPT"
     gene            complement(5930..6667)
                     /locus_tag="PL014_08355"
     CDS             complement(5930..6667)
                     /locus_tag="PL014_08355"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT57139.1"
                     /translation="MIDVRWTWAFLDTPRPLADRSWAYWALVTGWSVSAPRGEHGEFA
                     TLVPTDGDPWVKVQAVGDGVGGIHLDLDVDDVRAAADEATRLGAREIGTLGDPEIVVI
                     MRSPGGLVFCLTTWQGDSVQVRTGQSQLIDQVCLDLPTGAHDAETTFWEQLTGWAWRP
                     TDVAEFSCLERPDGIPLRLLFQRLGEVDGPVRAHVDLACVDRYATQARHVEAGAQVVS
                     VRDFWTVLRDPVGRAYCLTDRSPTSGG"
     gene            complement(6664..7677)
                     /locus_tag="PL014_08360"
     CDS             complement(6664..7677)
                     /locus_tag="PL014_08360"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOT57140.1"
                     /translation="MTLPPLPDLLAGMRVVAIPMRVRFRGVTTREVALIRGPHGWGEF
                     GPFLEYGPAEASRWLACAIESAHHPWPDAVRTTVSVNATVPAVAPGAVAGVLARFPGC
                     TTAKVKVVERGQSLADDVARVAEVRAAMGAAGRVRIDANGGWSVDEAVAALGALARYD
                     LEYVEQPCATVEELRDLRIALARAGVDVPIAADESIRKAEDPLRVRDLEAADLIVVKV
                     APLGGVRSALAIVQECGLPAVVSSALDTSVGISLGVALAAALPSLEHACGLGTVALLA
                     GDVTPSPLVPDHGSLTAGRVDVGEALLDRWAAPPERVTWWADRVAACHAVLAAAPQGG
                     GMP"
     gene            complement(7711..8637)
                     /locus_tag="PL014_08365"
     CDS             complement(7711..8637)
                     /locus_tag="PL014_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57141.1"
                     /translation="MTNDNVVRFGSRRGGDEWGRRPGDLGFRGIGDEPFDEHTGRYDE
                     YGLSRYDDEPRGSDLGTLTNVVLVGSQVIDVTCHPVAGSGYECAVLDLEQRGLYQRPI
                     SHEPPPAPPPPPPHERQLAWLATIVGGEEALLALESTPLPDEPLDLSGVPRHLHTRVE
                     AIAGRFDPWVSLAMGDEGLTAARRLLVRAVAAAPGLLRSDRDDIAAGAVVWAAAKGND
                     LVGQNRPLRASVIQDLAGLLTSPGQRGAAFAHALTGVGTSYGQADWLYGGLRPNVIPL
                     GSPDLLLGRFRQRLITFRDIALQLRARTPVAG"
     gene            8856..>9099
                     /locus_tag="PL014_08370"
     CDS             8856..>9099
                     /locus_tag="PL014_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT57142.1"
                     /translation="MSAIPGVSQTFDPAAWAEVPGFEDLTDITYHRAVDLGCVRIAFD
                     RPEVLNAFRPHTVDELYRVLDHARRTADVGVVLLTGN"
BASE COUNT         1337 a         3390 c         3233 g         1139 t
ORIGIN      
        1 ttgtcgcaag gtgagggcgc gcgcccgaaa tcgccccccg gcatccctag accaccccga
       61 ccttgtgcga cttgtgtgcg gcttcggcgc tcaccgggtg cagaaccgca ccgaagtcgc
      121 acgcagtcgg ttcagggggt gagtgccgcc gccgcgaggg tgcgcaggtc ggtgtgcgcc
      181 gcccggtgcg tggcgcggtc gacccgcact tcgacgaccc ggatgccgtg gtgcggctgg
      241 gcgaccgcgt ccgtcagatc cgcgctggtc gccaccacct cgtgccggat gccgtgcgcc
      301 tggcacagtg cccccagatc ggttgcggtc ggggtgccga agacgcgctc gaagtcggcg
      361 gcccgctcgg gcgcccctgg ttcgagggtc gtgaagatgc cgccgccgtc atcgttgacg
      421 acgacgagcg tgaggtcggg cacctgctcc cccggcccga tgagcagccc gttggtgtcg
      481 tggaggaagg tcaggtcgcc caccagcgcg acggtcggga ccaggtcgcg ggccagggcg
      541 acaccaaccg ctgtggagac caccccgtcg atgccggcga gcccgcggtt ggcgagcacc
      601 tcgtcggtga tcgagcccgc gatggccagg tcgaggtcgc gcaccgggtt gctcgggccg
      661 agcacgagca cgccgtcacc ggggaggccg tcgacgacgg cgctggcgac cgcgaggccg
      721 ctcggccacg ggaacggctg cgcagcaacg gcatccgcga gtcgtgcggc ggcactgcgc
      781 cactgttgcg cccagccgtg gtcgtcatcg ggagtggtcg ggccccccag gcgcagggcg
      841 gccggcgggt gcacggctgc ggcgacgtgg gacgggtcgg gccactggct gccctcggtg
      901 acgacctcga cgaccaggcc gggccggcgc accagtgccc cgacggcacg ggagagggtc
      961 acccgcccga cgacgacgac ccggtcgggg gcatgcgcgt cgagccagtc ggtgacggtg
     1021 agcaggaggg ggccgtgggg cagcgcctcg gcccgcaggg cggcggtgcc gaagggctcg
     1081 gcgacgatcg ggtggccgcg ttcggtagcc caggctgcgg cgcgctcccc gagggcgggg
     1141 tcgcgggtgt cgccgacgac gacgagagtg cgcgcgacct cgtccagtgg ggtgacgggg
     1201 tgtgacggat gtgaccagtt ggtcacttcc gtcacgcctg tgcggaccca gggggcgcgg
     1261 tccgcccggc cgtcgaggtt gtcgggccag tctgcggtcg ggtctggggt cggctcgttg
     1321 gtctggccgc tggcggagcc gccgacggcc tcatcagcca ggtccggggt cacgtcgggg
     1381 gccaggtccg gaaccagcgg ctcgcgcaac gcgacgttga ggtgcaccgg cccgggctcc
     1441 ccgccgatgt gacccgctgc cgccgcgagc gcccggcaca ccgtgctgcg ccagaacggc
     1501 ccgagcccgg ggcgttcgac aggggccggc aggtcggcgg accagcgggt ggagccgccg
     1561 aagatcccgg gctgggccgt ggtctggttg gcaccggtcc cgcgcagttc gtggggtcgg
     1621 tcggcggaca cgacgacgag cgggacgcct gcgtggtgcg cctcgagcac cgcagggtgc
     1681 agattggcga cggcggtgcc ggatgtcgtg acgatcgcga ccggcatccg cgatccgcgg
     1741 gccagaccga gcgcgaggaa cccggcgctg cgctcgtcga cccgcacgtg cagccgcagg
     1801 tgaccggcgc gctcggccgc cacgagcgcg taggccaatg gcgctgaccg cgacccgggg
     1861 cacaggacca cctcgcgcac cccaccacgg acgagctcgt cgaccacgac ggtcgacagg
     1921 gcctgggacg ggttcacggg cgtgagtctg ccactgggcg agccgcccgc agccgggtca
     1981 cccagatggc ggtgggcgca gcggaggtca tggtggcatc gtggcacctg ctcgctcgat
     2041 gacgtggtcg tgaaccgagc gcacctgacg gccgaggacg tcgccagcac cgacctgcca
     2101 gacgtcgagg ccgggcactg cgccccgagg tgcaccttcg gcagcccgga cggtctgcac
     2161 gtaggtggac aggcggtgcc ggtcagggtc cagtggcgcg ccccgtccgt cgcccggcgc
     2221 cacgatggtc gtccacgact gaccgcccgg gttgggggtg aggtcgagcc cgggcaccgg
     2281 gtcatcggcg tgctcgatcg agagcacctt caccgtcggc ggcaccggga agagggcaac
     2341 cggcgcacca ctggtgacga cgtgggtgac ccggttgccg gtccgaaacg ccgggtcgct
     2401 ggccagcgcc gcagcgagga tgcccccctg cgagtgcccg acgagcatca cgggatcctg
     2461 cggtgaggac cgccccgacc cggctcgcga acgggccagt gccgcggaca ccccggccgc
     2521 cgcgatcgtc acgtcaccgg tgagtgcgcg cacgtcggtg gtgaggtcga aggggttggc
     2581 ccccggccgc ggtgaccact cctgggtgcc gggaacgatg acgacccacg ccgaaccgcc
     2641 gtcacctcgg gtcgtctcca gaacccgcac ccgccccccg tcgaggccct cgcccgcagc
     2701 aacgaggtcg gccaccccga gcacccgacc agtcacgccg ccatgagcca ccaaggggcc
     2761 ggtgagcgtc gacggcacgg cccgcacgat cgggtcgctg ccgttcaccg gaccgtcgac
     2821 gaaccacccg acccgaccag cgacgtcagc gcccgcggca accgctgaga gcaccccggc
     2881 gacgccccgc tcgacctcga cgtacatccg agccgcggac ctcagcgcag ccgccagccc
     2941 gtcgagctcg agggcatcac cccacaggcc taccggcccg atgagggcca gtgcccgacc
     3001 ctcaaggacc aaccaccggg cgaccagcac ggcctcaccc ggtgatcgca cccgcggggg
     3061 ccccaccccg gccacgacgg cggcgagccg cccggcatcc tcacgaagct cgtccgcgat
     3121 ggcgagcacc cgttgggccg ccgcggtgag cgtctcggag cggacatcga ccctcacgat
     3181 gccagccccg acccgaggtc acgagccgca gactcggccg cgaagagccc tgccagttcg
     3241 tgacatgcct gcgcgacgaa cagcaactcg gcctcggtag ccgccagccc ggccacccgc
     3301 cgctcgaccg ccccccgata ggtctgggcg gccccgccct gccaccagac gagggcaaac
     3361 ctcgagacgg ccgcccgacg ctcccgcaac cgatcggccg ctgcaccggc ctcccgcgac
     3421 agccgctgca actcctccac cgggtccttg cccacgcgct tcccctcgcc atccccgggc
     3481 gcgaccgccg tccggtcgcg ccccctgaag ccaccttcac ccgcgcgacc cagccagacg
     3541 aatcgcgagc gcccacctgt ggacgaccgg gctccgcgcg caagcctgtg gacgaccacg
     3601 cctacgctga cgccatgccg caccacgatc tcgtcatcat cggcagcggc tcggggaact
     3661 cgctgctgac ccccgagctc gacggcctcg acgtcgccat cgtcgagggc ggcaccttcg
     3721 gggggacgtg cctgaacgtc ggctgcatcc cgaccaagat gctcgtcctc cccgccgacc
     3781 gcatcgtgga tgccgcggac gccgcccgcc tcggcgtcac cttcgcccag cccacggtgg
     3841 attggcccgg catccgcgac cggatcttcg ggcgactcga tgcgatctcc gctggtggcc
     3901 gcgcctaccg tgacctgcag gcgcacacga cggtgtacgc ggcgcacgcc cggttcaccg
     3961 gcccgcgcca gctcgcgttg tccacgggtg aggtgctgac ggcggaccgg gtcgtcgtcg
     4021 ccgctggtgc gcacgccgtc ggcctgcccg tcgaggggct gcgcagccct gaccccgccc
     4081 ggggcctgca cacctcggac acggtcatgc ggctggatgc cctgcccccg cggatggtcg
     4141 tcatcggcgg cgggttcgtc gcctgcgagc tcgcgcacgt gttctcgtcg ctgggggtcc
     4201 aggtcaccca ggtgcagcgc gggcccagcc tgctgcggca ccacgagccc gccatcgcgc
     4261 agcggtacac gcaggtcgcg gcgcaccact acgacgtgcg gctgtccgcc accccgacct
     4321 cggcgcaccg caccgacggc gtctggcgca tcgacctcga caccgcgtcc ggccccgccc
     4381 acgtcgaggc cgaggtcgtc ctgctcgccg tcggtcgccg ccccagcacc gacggtctcg
     4441 acccggcggc tggtgggttg gaggtgcacg ccgacggtcg cctcgccgtc gacgcacagc
     4501 tgcgcaccac cgcggatgcc gtgtgggcgc tcggcgacgt ctgctcgccc caccagctca
     4561 agcacgtcgc caaccacgag gcgcggatcg tcgcgcacaa cctcgcagtc gacctcggac
     4621 gacgtgcggg accagttcag cgcacggatg accggccggt accgcacgcg gtgttcggcc
     4681 acccgcaggt cgcggcgttc ggcccgacct cggccgacct cgatgccgct ggcacgacgt
     4741 atgtcagcgc gacgcaggcg ttcggcgaca ccgcctacgg gtgggcgctc gaggacacca
     4801 ccggcctgct caccatccac gccagccccg aaggccgcat cctctctgcc cacgcggtcg
     4861 gtccgcactc ctccacgctc atccagccgc tcatccaggc ggcgagcttc ggccagcacg
     4921 cccacgaggt cgcccgcggc cagtactgga tccacccggc cctggccgag gtcgtcgaga
     4981 acgccctgct cggcctgccc ctggggtgaa ccgggaaggg gcccgtcgtg ggaccgctca
     5041 ggtggggatg agggtgttgg cgaagatctg ccacgcgagg acggacttcg cgacgaggct
     5101 gaggatgacg tactgccact caccgaacag gtagtcgcgc cagcgcccga cctggcggta
     5161 ctggagccac tggttgagcg cgaaggtgtt gaacagcacg aacaggctga tgaggatgcc
     5221 ccagacgaac cccggggggc cctggccgtc gagcacagcg atcgaggccc acaggtacgc
     5281 cccgatcgcc agccacgggc cgatgccggc gacgcagccg aaccagaacg gtgtccacca
     5341 ggcccgctcg ccccgggccg gcgactggtc gcgaccgtgc atcaggccgt tgttggagac
     5401 ctccatgagc cacccgaaga ggatcatcgc gatgttggcg aacgcgatgc cgatcagtgc
     5461 cgcgaggtcg gtgatgccgg tgacgagccc gatgaggacg atcatgagcg tcgacgacag
     5521 ggagtactcc acccaacgga agcggttgcg ccctcgctcg agctcgccga cgtagcgcgg
     5581 gaagccccac ggggaggcga tgacgaagtg gaagaacgcc gagaggaaca ggaagctcgc
     5641 cgtcgcccag gcgaggggga cgtcgagcac gcccgagagc gtcccggcct cgagaggtgt
     5701 cccgggtggg ccgccgaggg agaagaccga cacgggcagg gtgaagtccg tgctcagcgc
     5761 caccatggcc gcaccggaca ccaggtggag caggcccatg cccacgttga agcgccggag
     5821 cgaccgggcg cgctcgggag ggacggcgac ggagggagtc gagatctcca gcgggaaggt
     5881 gctcatgacc tcccttcccc cgcgggaggt cccgtggatg cacccggggt cagccgcctg
     5941 aggtggggct gcggtcggtg aggcagtagg cccgcccgac ggggtcgcgc agcaccgtcc
     6001 agaagtccct gaccgacacg acctgggctc cggcctccac gtgccgcgct tgggtggcgt
     6061 agcgatcgac gcaggcgagg tcgacatggg cgcgcaccgg accgtcgacc tctccgagcc
     6121 gttggaagag caggcgaagc gggatgccgt caggtcgctc caggcacgag aactccgcca
     6181 cgtccgtggg tcgccacgcc cacccggtca gctgctccca gaacgtggtc tcagcgtcgt
     6241 gcgcaccggt cggcaggtcg aggcacacct ggtcgatcag ctgggactgc ccggtgcgaa
     6301 cctgcaccga gtccccctgc caggtcgtga ggcagaacac gagcccgccc ggtgaacgca
     6361 tgatgaccac gatctccgga tcgccgagcg tgccgatttc acgcgcaccc aaccgggtgg
     6421 cctcgtctgc ggccgcgcgc acgtcgtcga cgtcgaggtc gaggtggatg ccgcccaccc
     6481 cgtccccgac cgcctggacc ttcacccagg ggtcgccgtc ggtgggcacc agggtcgcga
     6541 actcgccgtg ctcgccgcgc ggggccgaca ccgaccaccc ggtgaccagc gcccagtagg
     6601 cccacgagcg gtcggcgagc gggcgtgggg tgtcgaggaa ggcccacgtc cagcgcacgt
     6661 cgatcacggc atgcccccgc cctgcggagc agcggccagc acggcgtggc aggcggcgac
     6721 ccggtcggcc caccaggtga cccgctccgg tggcgcagcc caccggtcga gcagtgcctc
     6781 gccgacgtcc acccgcccgg ccgtgagtga cccgtggtcc ggcacgagtg ggctgggtgt
     6841 gacgtctccg gcgagcaggg ccaccgtgcc caacccgcag gcgtgctcga gcgagggcag
     6901 cgcggcggcg agcgccaccc cgagggagat gccgacgctg gtgtccaacg ccgacgacac
     6961 gaccgccgga aggccgcact cctgcacgat cgccagcgcc gagcgcacac ccccgagtgg
     7021 cgcgaccttg acgacgatga ggtcggcagc ctcgaggtca cggactcgca gcgggtcctc
     7081 ggccttgcgg atcgactcgt cggcggcgat cggcacgtcg acaccggcgc gcgccaaggc
     7141 gatgcgcagg tcacgcaact cctcgacggt ggcgcacggc tgttccacgt actcgaggtc
     7201 gtatcgggcc agggcgccca aggcggccac ggcctcgtcg acgctccacc cgccgttggc
     7261 gtcgatccgc acccggcccg cggcgcccat ggcggcacgc acctcggcca cccgcgcgac
     7321 gtcgtcggcc aggctctgcc cgcgctcgac gaccttgacc ttggccgtcg tgcacccggg
     7381 gaagcgcgcg agcacgccgg cgaccgcccc gggcgcgacc gctgggacgg tcgcgttgac
     7441 cgaaacggtc gtgcggacgg catccggcca ggggtggtgg gcgctctcga tggcgcaggc
     7501 gagccagcgc gaggcctcgg caggcccgta ctcgaggaac ggcccgaact caccccaccc
     7561 gtgcggcccg cggatgagcg cgacctcgcg agtggtcacg ccacgaaagc gcacccgcat
     7621 ggggatggcg acgacgcgca tccccgccag caggtccgga agcggtggca acgtcatgct
     7681 tcccaccgta agccgtggcc gcgcggccga tcagcccgcc accggggttc gggctcgcag
     7741 ctggagcgcg atgtcccgga aggtgatgag ccgctggcgg aagcgaccga gcagcaggtc
     7801 ggggctgccg agtgggatga cgttcgggcg cagaccgccg tagagccagt ctgcctggcc
     7861 gtagctcgtc cccacacccg tcagggcgtg cgcgaaggct gcccctcgtt gaccgggcga
     7921 ggtgagcaga ccggcgaggt cctggatgac gctcgcccgc agtggccggt tctgccccac
     7981 gaggtcgttg cccttggccg cggcccacac gacggctcca gcagcgatgt catcacggtc
     8041 gctgcgcagc agaccgggtg ccgcagccac agcacggacg agcagccggc gggccgcggt
     8101 caggccctca tcgcccatcg cgagcgacac ccacgggtcg aacctcccgg cgatggcctc
     8161 gacccgtgtg tgcaggtgcc gcggcacacc cgacaggtcg agcggctcgt ccggcagcgg
     8221 tgtggactcg agtgccaaca gcgcctcctc tccccccacg atcgtcgcca accacgccaa
     8281 ctggcgctcg tgcggcggcg ggggcggcgg cgcaggaggt ggttcatggc tgatgggtcg
     8341 ctggtacagc cctcgctgtt cgaggtcgag caccgcgcac tcgtacccgc tgcccgccac
     8401 cgggtggcac gtgacgtcga tgacctgtga gcccaccagc accacgttgg tcaaggtccc
     8461 gaggtcggac ccacgcggct cgtcgtcgta ccgcgaaagg ccgtactcgt cgtaccgccc
     8521 ggtgtgctcg tcgaatggct cgtcaccaat acctcgaaac cccaggtcgc cgggcctgcg
     8581 gccccactca tcgccacccc ggcgcgagcc gaacctcacc acgttgtcgt tcgtcatgac
     8641 tcccccgttc cgcgccccct ggcgcgctct tgacctgatc gtggcacgag gggccgacat
     8701 cgccgatgga gttgtccaca ggccgccggg gcgcggtcgt ctcgacgtcc cgggcgaccc
     8761 tgctcacctt cgaccggagg tttcgccgct tccgcggcct cgacgtgagc ctcctgcgtg
     8821 ggtagccgtc accgggcgcg gctagccttg ccgccgtgag cgcgatcccc ggagtgagcc
     8881 agacgttcga ccccgctgcc tgggcggagg tgcccggttt cgaggacctc accgacatca
     8941 cctaccaccg cgcggtcgac ctggggtgtg tgcgcatcgc gttcgaccgc cccgaggtgc
     9001 tcaacgcctt ccgcccgcac accgtcgacg agctctaccg ggtgctcgac cacgcacgcc
     9061 gcacggctga cgtcggcgtc gtcctgctga ccggcaacg
//
LOCUS       DAOSOC010000097         9098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7595_length_9098_cov_7.878912, whole genome shotgun sequence.
ACCESSION   DAOSOC010000097 DAOSOC010000000
VERSION     DAOSOC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7595_length_9098_cov_7.878912"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PL014_08375"
     CDS             <1..554
                     /locus_tag="PL014_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT57143.1"
                     /translation="HSGAGKSTLLSVVAGIVTPDAGEVLVDGRRVGGVVAAGVAEAVA
                     AGVALIPQGNGLAAMLTARENVLVPLLARGVAAAHAPARADAALDAVGLGEVFSHLVE
                     ELSGGQQQRVAVARALALDCRVLLADEPTSELDHTNREVVLRLLRARASQGAAVVMAT
                     HDPEAAAAADRVVHLDDGVLTTP"
     gene            complement(668..3193)
                     /locus_tag="PL014_08380"
     CDS             complement(668..3193)
                     /locus_tag="PL014_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HOT57144.1"
                     /translation="MSTAQTSADPLDRSRFPADPWRLVECDPERADLGVTETLFTVAN
                     GYIGLRGNPEEGRHAHEHGTYINGFHETWPIRHAEAAYGFARTGQTLVNAPDAKLMKL
                     YVDDEPLVIGTADLESYERSLDFRDGVLRRSLVWRTPSGKRVKVVSTRMVSMTQRHLV
                     LLTLEVSMLSGDAPVVVSSQLLNRQDGVDEYHGQSAADTGLADPRKAAAFEGRVLEPR
                     LQMAKDDRMMLGYQCANSRMTVAVAADHAVSTPDPYEVVMSDEPDLTKMVFRVDAREG
                     STLRIEKTVAYHTSRGLPVRELSDRCRRTLDRAARVDNEHWLAEQRDWFDAFWASADV
                     EVDTGDETSDAVQQAIRFNLFTLAQASARADGLGVAAKGVTGSGYEGHYFWDTEVYVA
                     PFLTYTRPELARNLMLFRSRMLPAARERAAEMSQRGALFPWRTINGEEASAYYAAGTA
                     QVHIDADVAHALVQYVSATGDEELLVRHGLAILVETARLYADLGFWRSNGERSFHIHG
                     VTGPDEYTTVVNNNLFTNVMARYNLEQAVSWVRWAQEHHADEYARLAHRLKVTEDEVT
                     EWAACAAGMHIPFDEGLQIHPQDDFFLDREVWDLSQTPDELRPLLLHYHPLVIYRFQV
                     LKQADVVLALFLQGNRFTADEKRRDFEYYDPITTGDSTLSAVVQSVVAAEVGYHEVAH
                     AYFVDALYVDLANAHANTVDGLHIASTGGVWCALVNGFGGMRDHEGQLSFDPRLPADW
                     RSLTYRLTWRGTRLVVDLTPTGLRLRSVEGEQPVPVSVAGIRHVVEPGSTLEVPLDGH
                     GPQIPGLLPKRPPTGGTRTDGSRITAGVPTAIPLPEAGDPPPA"
     gene            complement(3190..3921)
                     /locus_tag="PL014_08385"
     CDS             complement(3190..3921)
                     /locus_tag="PL014_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT57145.1"
                     /translation="MNPVTWTDHDSVLFDLDGVLTPTAEVHMRAWAEMFNAFLSSWHG
                     PAADTSAYTDDDYFAHVDGKPRYDGVRDLLTARGIDLPEGDPSDAPTLDTVCGLGNRK
                     NDAFNAVLERDGVSPFPGSVALLDHLRDLGMPLAVVSSSANAPAVLEAAGLAGRFVTV
                     VDGRVATELGLPGKPAPDTFVHAATACGATPARSVVVEDAVSGVRAGAAGEFGLVIGV
                     DRGAGAQTLTDAGAHLVVADLGELA"
     gene            4029..4544
                     /locus_tag="PL014_08390"
     CDS             4029..4544
                     /locus_tag="PL014_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT57146.1"
                     /translation="MSQPVRLPFDPIARASQLWRDRWGRESHASSMATATSIMRVQQL
                     LLAQFDAIAGRHGLTFARYEALVLLTFSRDGRLSMSRIGERLMVHPTSATNIVQRLVA
                     QGFVERVPNPADRRGAFAVITDAGRAAMEAVTRDLEAADFGLSMLDAEQHDVLFGLLR
                     EVRVGAGDFEA"
     gene            4665..>4887
                     /locus_tag="PL014_08395"
     CDS             4665..>4887
                     /locus_tag="PL014_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57147.1"
                     /translation="MTTIVKSVVGALLAVLGLVLTVIGAWFATQLGSSGTAQFTARPA
                     TTDPVIIRPSVLNRVESDVVVTATPAEGAT"
     assembly_gap    4888..4987
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5145..6146
                     /locus_tag="PL014_08400"
     CDS             5145..6146
                     /locus_tag="PL014_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57148.1"
                     /translation="MTRRTTSAVAAATLASALLLTGCGTQDALVGLRPAPAEKSVSAP
                     LGDEGAADIAARVLAQAEAPIEGDAKKAAAARAVFLAGDALTVANAMAARGAAPAATP
                     DLAVAPKPTIVGQSQGRAWPRAIVASTLDEATNTQMLHVLVSEKPDQPFKIVSSVPMF
                     AGATLPAVAPENAGSPMVEVTDGEGLPATPEAVIKAYAAALAFPKPVATKAVSVDNAF
                     ANSLKTTAAAQAKALGKLGTLTQVHTPELKNAVSFRLADGGVVTFGLMKRTDTLAVTK
                     AAKELVLPAEYAKLTGKKKVTKSLTLNNLETFVMLIPPTGQASVIGGAELLVSGKAL"
     gene            6181..7167
                     /locus_tag="PL014_08405"
     CDS             6181..7167
                     /locus_tag="PL014_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT57149.1"
                     /translation="MSETTPPAGTRGAVDLSSVAGLTAPSAATAGSGMPAGPVPGQPP
                     AARPAVPGGLVVEVTASNLQEALNRTLQVPGVLVLWGSAYSATRELLDTVVSVAASLE
                     GRVLVLSADLSASPELLQVFQPLLVQAFGQPSVPATFGLLQGQPVPLFPGIQPEAEVR
                     AVIDQLLQAAVQNGIAGRVELEPVEGAEDDALPPLHEAAFQAIERGDLAGAAAAYEQA
                     LAENPKDHDAELGLAQVGLMQRTTGVDLTAARAAAAADPADVDAACVVADLDVLGGHV
                     EDAFARLLDLVRTSEGADRDKARTHLLELFSVVGNHDERVRKGRTALMSALF"
     gene            7173..8216
                     /locus_tag="PL014_08410"
     CDS             7173..8216
                     /locus_tag="PL014_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT57150.1"
                     /translation="MAATLIDDHLRFRLDDVDPAVTAVVLQCERAVPGPREFTRDDAG
                     WCLDLPVPSLQRIEYRFAVGRGDAVDVVLDPANPLTVGTAFGDRSVVELPGYAPPWWL
                     SAPAVPGRLDPHTLTGETTHEVPVTVWIPQDLPDTEPAPLLLVHDGPEYDQLASITTY
                     SAALIATGVLPPHRVALAAPVIRDAWYSGSPQYLRTIAATGLDGLGERYAVRGPVVVA
                     GASLGGLTAVLLGLLAAPRVGGVYSQSGSFFQVRHDLDESGYPFFGRISRLVQSVLDM
                     RETEHPLVVGMSCGAIEENMANNRDMAAALRRAGHTVELTEVADLHNYTAWRDALDPG
                     LTHVLRTVWGETG"
     gene            8270..9016
                     /locus_tag="PL014_08415"
     CDS             8270..9016
                     /locus_tag="PL014_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT57151.1"
                     /translation="METSAVRLQVPGHDVDLEVVRYGAWGRPVLLFPSEGGSARDAQG
                     NGMLDAVRPLIEAGRVSLFCVDSMDGWTWSANDQPTEERARRAQTYTAWLSQQVLPWV
                     QEQVGGGQEIITVGVSLGAYHAVHLTFQRADVAPMAIGLSGNYDPTSWNGWGELGDAT
                     YFANPTAYVANMDGDHLDWIRSRVSVLLVVGEGPFEVSPTRSLPATREFAALLADKGI
                     RYELDVWGHDSAHDWPWWQRQLAHHLQRFV"
BASE COUNT         1232 a         3170 c         3252 g         1344 t
ORIGIN      
        1 gccactccgg ggcgggcaag agcaccctgc tctcggtcgt ggccggcatc gtcacgccgg
       61 atgccgggga ggtgctcgtg gatggccgcc gcgtgggtgg ggtggtcgcg gcgggggtgg
      121 ccgaggctgt cgcggccggg gtggcgctca tccctcaggg caacgggctc gccgcgatgc
      181 tgacggcacg cgagaacgtg ctggtcccgc tgctcgcccg tggggtggct gccgcgcacg
      241 cgcctgctcg ggcggacgcc gcgttggatg ccgtcgggct gggtgaggtg ttctcgcacc
      301 tcgtcgagga gctgtccggc ggacagcagc agcgcgtcgc cgtggcgcgg gcgctggccc
      361 tggactgccg cgtgctgctg gccgacgagc cgacctccga gctcgaccac accaaccgcg
      421 aggtcgtgct gcgcctgctg cgcgcgcggg cttcgcaggg ggcggcggtc gtcatggcca
      481 cacacgaccc ggaggcggcg gctgcggccg accgggtggt gcacctcgac gacggcgtcc
      541 tcaccacccc ctgacccggc atccgccgag ctttctcgcc cgatgccaca aggttcaggt
      601 tcgcaccaga acggttgtgg tgcgaaggtg aaggtcctgg caccgggcga gaaaggtgat
      661 gccggggtca ggccggggga gggtcgcccg cctcgggcag gggaatggca gtcggcaccc
      721 cggccgtgat gcggctgccg tcggtgcggg tgccgccggt cggcgggcgc ttgggcagca
      781 gaccggggat ctgcgggccg tgcccgtcga ggggcacctc gagcgtgctc cccggctcca
      841 cgacgtgccg gatgccggcc acgctcaccg gcacgggctg ctcgccctcg accgagcgca
      901 ggcgcagccc ggtgggggtg aggtcgacga cgagtcgggt gccccgccac gtcagccggt
      961 aggtgagcga gcgccagtcg gcgggcaggc gcgggtcgaa ggagagctgg ccctcgtggt
     1021 cgcgcatccc gccgaagccg ttgaccagcg cgcaccacac cccgccggtg gaggcgatgt
     1081 gcagcccgtc gacggtgttg gcgtgggcgt tcgccaggtc gacgtacagg gcgtcgacga
     1141 agtaggcgtg ggcgacctcg tggtagccga cctcggctgc cacgacggac tgcaccacgg
     1201 ccgacaacgt cgagtcgccg gtggtgatcg ggtcgtagta ctcgaagtcg cggcgcttct
     1261 cgtcggcggt gaagcggttg ccctggagga acagggcgag cacgacgtca gcctgcttga
     1321 gcacctggaa gcggtagatc accagcgggt ggtagtgcag cagcagaggc cgaagctcgt
     1381 ccggggtctg gctgaggtcc cacacctcgc ggtcgaggaa gaagtcgtcc tgggggtgga
     1441 tctgcaggcc ctcgtcgaac gggatgtgca tgcccgccgc gcaggccgcc cactcggtga
     1501 cctcgtcctc ggtgaccttg aggcgatggg ccaggcgggc gtactcgtcg gcgtggtgct
     1561 cctgcgccca gcgcacccac gacaccgcct gctcgaggtt gtagcgggcc atgacgttgg
     1621 tgaacaggtt gttgttgacc acggtcgtgt actcgtcggg cccggtcacg ccgtggatgt
     1681 ggaacgagcg ttccccgttg ctgcgccaga agccgaggtc ggcgtagagc cgggcggtct
     1741 cgacgaggat ggcgaggccg tgacgcacga gcagttcctc gtcgccggtg gcgctgacgt
     1801 actgcacgag cgcgtgcgcg acgtcggcgt cgatgtgcac ctgggcggta ccggcggcgt
     1861 agtacgccga ggcctcctca ccgttgatgg tgcgccaggg gaagagggcc ccgcgctggc
     1921 tcatctccgc agcccgctca cgagcggcgg gcagcatccg gctgcggaag agcatgaggt
     1981 ttcgggccag ctcgggccgg gtgtaggtga ggaagggggc gacgtagacc tcggtgtccc
     2041 agaagtagtg gccctcgtac cccgagccgg tgaccccctt ggccgccacc ccgagcccgt
     2101 cggcccgggc gctggcctgg gccagggtga acaggttgaa gcgaatggcc tgctgcacgg
     2161 catccgaggt ctcgtctccg gtgtcgacct cgacgtcggc ggaggcccag aacgcgtcga
     2221 accagtcacg ctgctcagcc agccagtgct cgttgtcgac ccgggctgcc cggtccaggg
     2281 tgcggcggca ccggtcggac agctcacgaa cgggcaggcc gcgcgaggtg tggtacgcca
     2341 cggtcttctc gatgcgcagc gtgctgccct cgcgggcgtc gacgcgaaag accatcttcg
     2401 tcaggtccgg ctcgtcgctc atgacgacct cgtacgggtc gggtgtcgac acggcgtggt
     2461 cggcggccac ggcgacggtc atccgggagt tggcgcactg gtagcccagc atcatccggt
     2521 cgtccttggc catctgcagc cgcggctcga gcacccggcc ctcgaaggcc gccgccttgc
     2581 gtgggtcggc caggccggtg tcggcggccg actggccgtg gtactcgtcg accccgtcct
     2641 gacggttcag cagctgggag gacaccacca ccggggcgtc gccggagagc atgctcacct
     2701 cgagcgtgag caggacgagg tggcgctggg tcatcgacac catccgcgtc gagacgacct
     2761 tgacgcgctt gcccgacggg gtgcgccaca ccagggatcg acgcaggact ccgtcacgga
     2821 agtccagcga ccgctcgtag ctctcgaggt cggccgtgcc gatgacgagc ggctcgtcgt
     2881 cgacgtagag cttcatcagc ttcgcgtcag gggcgttgac cagggtctgg cccgtgcggg
     2941 cgaacccgta ggccgcctcc gcgtgccgga tcggccaggt ctcgtggaag ccgttgatgt
     3001 acgtcccgtg ctcgtgggcg tggcgaccct cctcggggtt gccacgcagc ccgatgtagc
     3061 cgttggccac ggtgaacagc gtctcggtga ccccgaggtc ggctcgttcg gggtcgcact
     3121 ccaccagccg ccacgggtcg gcggggaagc ggctgcggtc cagcgggtcc gccgaggtct
     3181 gcgcggtgct cacgccagct cgccgaggtc cgcgacgacg aggtgcgccc cggcatccgt
     3241 gagggtctgc gcgcctgccc cgcgatcgac cccgatgacg aggccgaact cgccggccgc
     3301 cccggcgcga acgcccgaca cggcatcctc gacgacgacg gagcgggcgg gtgtggcacc
     3361 gcacgccgtg gccgcgtgca cgaaggtgtc gggagccggc ttgcccggca ggccgagctc
     3421 ggtggcgacc cggccgtcga cgacggtgac gaagcgtccg gccaggccgg cggcctccag
     3481 caccgccggg gcgttcgccg acgacgagac gaccgcgagc ggcatcccga ggtcgcgcag
     3541 gtggtccagc agcgccaccg atcccgggaa cggtgacacc ccgtcgcgtt cgaggacggc
     3601 gttgaaggcg tcgttcttgc ggttgccgag tccgcacacg gtgtcgaggg tgggggcgtc
     3661 gctcgggtct ccctcgggca ggtcgatgcc gcgtgccgtg agcaggtccc gcacgccgtc
     3721 gtagcgcggc ttgccgtcga cgtgagcgaa gtagtcgtcg tcggtgtagg ccgaggtgtc
     3781 ggcggcgggg ccgtgccatg aggagaggaa ggcgttgaac atctcggccc aggcccgcat
     3841 gtgcacctcc gccgtggggg tgaggacccc gtcgaggtcg aagaggaccg agtcgtggtc
     3901 ggtccaggtc accgggttca taccgctcat gaggggcaac gctagggact gcgagagcgc
     3961 ttgcgcaatc cgttggtgcc gccttcccga gagtcggtcc ggggcgccgc tgggctaccg
     4021 tgctcggtgt gagccagccg gtgcgcctgc ccttcgaccc gatcgctcgg gcgagccagc
     4081 tgtggcgcga ccgctggggc cgggagtcgc acgcctcgtc gatggccacg gcgacgtcga
     4141 tcatgcgcgt ccagcagctg ctcctcgccc agttcgacgc catcgcgggt cgccacgggt
     4201 tgaccttcgc gcgctacgaa gcactggtgc tgctcacctt cagccgcgac gggcgcctct
     4261 cgatgtcccg catcggggag cgcctcatgg tgcacccgac gagcgcgacg aacatcgtcc
     4321 agcgcctggt ggcccagggg ttcgtcgagc gggtgcccaa ccccgccgac cgtcgaggcg
     4381 cgttcgcggt gatcacggat gccggtcgcg cggcgatgga ggcggtgacc cgtgatctgg
     4441 aggcggccga cttcgggctc tcgatgctcg acgccgagca gcacgacgtg ctgttcggac
     4501 tgcttcgtga ggtccgcgtc ggggcaggcg acttcgaggc ctgacgggag ttctcccgcc
     4561 gtgggatttg tcgggagggt ggggcgtttg tcatggtgga gggaacgccg tggcgcgttc
     4621 tccgcgacgg tgcccccctt gtcaacccga aggaccccat ccccatgacc acgatcgtga
     4681 agagcgtcgt cggtgcactg ctggccgttc tcggcctggt gctcaccgtc atcggcgcgt
     4741 ggttcgcgac ccagctgggg tcctcgggca ccgcgcagtt caccgcccga ccggcgacga
     4801 ccgacccggt gatcatccgg ccctcggtgc tcaaccgcgt cgagtccgac gtcgtcgtga
     4861 ccgccacccc cgccgagggt gccacggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnncgc tcgagaccgt gaccttcacc gtcacggaca agacgtggtt cgtcgaggcc
     5041 gtcgtcgccg tcctggccgg actcttcctc atcgtggccg gcgtggcgtt ggcctggccc
     5101 agccgccgat ccatcgacga agcgcccacc gaggaggtca cccgatgacc cgccgcacga
     5161 catccgccgt tgccgccgcc accctggcca gtgcccttct cctcaccggc tgcgggacgc
     5221 aggacgccct cgtgggcctg cgcccggcgc cagccgagaa gtccgtctcc gcgccgctcg
     5281 gggacgaagg cgccgccgac atcgccgccc gggtcctcgc gcaggcggag gcgcccatcg
     5341 agggtgacgc caagaaggcg gccgcagccc gtgcggtctt ccttgcgggt gacgccctga
     5401 ccgtggccaa cgcgatggcg gcccgtggcg cggcacccgc ggccacccct gacctcgcgg
     5461 ttgcaccgaa gccgacgatc gtcggccagt cgcagggtcg ggcctggccc cgtgccatcg
     5521 tcgcgtcgac cctcgacgaa gcgacgaaca cccagatgct gcacgtcctg gtctccgaga
     5581 agcccgacca gccgttcaag atcgtctcga gcgtgccgat gttcgccggc gccacgcttc
     5641 ccgcggtggc cccggagaac gccgggtccc cgatggtgga ggtcaccgac ggcgaggggc
     5701 tcccggccac gccggaggcg gtcatcaagg cctacgcggc agccctggcc ttccccaagc
     5761 ccgtggcgac gaaggccgtc tccgtcgaca acgcgttcgc caactccctg aagaccaccg
     5821 ccgcggccca ggccaaggcg ctcggcaagc tcggcaccct gacccaggtg cacacgcccg
     5881 agctgaagaa cgccgtgtcc ttccgcctcg ccgacggagg agtggtcacc ttcggcctga
     5941 tgaagcgcac ggacaccctg gccgtgacca aggctgccaa ggagctcgtc ctgcccgccg
     6001 agtacgccaa gctgaccggc aagaagaagg tgacgaagtc gctcaccctg aacaacctcg
     6061 agaccttcgt catgctcatc cccccgacgg ggcaggcatc ggtcatcggt ggggccgagc
     6121 tgctcgtctc cggcaaggcc ctgtgatcgc gcagccctgc gcgtttgaga gaatctgcgc
     6181 atgagcgaga ccaccccgcc cgcgggtacc cgcggcgccg tcgacctgtc ctccgttgcc
     6241 ggcctgacgg ccccctccgc agccacggca ggcagtggga tgccggccgg cccggttccc
     6301 ggccagccgc cggcagcgcg ccccgcggtt cccgggggtc tggtcgtcga ggtcaccgca
     6361 agcaacctcc aagaggcact gaaccgcacc ctgcaggttc ccggcgtcct ggtgctgtgg
     6421 ggctcggcct actccgcgac ccgtgagctg ctcgacacgg tcgtctcggt cgcggcgtcg
     6481 ctcgagggtc gcgtcctcgt gctcagcgcc gacctgtcgg catcccccga gctgctccag
     6541 gtcttccagc cgttgctcgt ccaggcgttc gggcagccga gcgtgccggc gaccttcggt
     6601 ctgctccagg gtcagccggt gccgctcttc cccggcatcc agcccgaggc cgaggtgcgt
     6661 gcggtcatcg accagctgct ccaggcagcc gtgcagaacg gcatcgccgg tcgcgtcgag
     6721 ctcgagccgg tcgagggcgc cgaggacgac gcgctcccgc cgctgcacga ggctgcgttc
     6781 caggcgatcg agcgcggtga ccttgctggg gcggccgccg cctatgagca ggcgctcgcc
     6841 gagaacccca aggaccacga cgccgagctc ggcctggccc aggtgggcct gatgcagcgc
     6901 acgaccggcg tcgacctgac ggcggcccgg gctgcagccg cggccgaccc tgccgacgtc
     6961 gacgccgcgt gcgtcgtcgc cgacctcgac gtgctcggtg gccacgtcga ggacgccttc
     7021 gcccggttgc tcgacctcgt gcgaaccagc gagggtgctg accgcgacaa ggcccgcacg
     7081 cacctgctcg agctgttctc ggtcgtcggc aaccacgacg agcgggttcg taaggggcgc
     7141 accgccctga tgagtgccct cttctgatcc tggtggcagc gaccctcatc gacgaccacc
     7201 tccggttccg gctcgacgac gtcgacccgg ccgtcacggc cgtcgtcctc cagtgcgagc
     7261 gagccgttcc cggcccgcgc gagttcaccc gtgatgacgc gggctggtgc ctcgacctgc
     7321 cggtgccgag cctgcagcgc atcgagtacc gcttcgccgt cggccgcggt gacgccgtcg
     7381 acgtcgtgct cgaccccgcc aaccccctca ccgtgggcac cgcgttcggg gaccggtcgg
     7441 tggtcgagct gcccggctac gcgccgccgt ggtggctgtc ggcgcccgcc gtccccggac
     7501 ggttggaccc gcacaccctc accggtgaga ccacccacga ggtgcccgtc accgtctgga
     7561 tcccgcagga cctgcccgac accgagcccg ctccactgct gctcgtgcac gacggccccg
     7621 agtacgacca gctcgcgtcg atcaccacct acagcgcagc cctcatcgcg acgggggtgc
     7681 tgccacctca ccgcgttgcc ctcgcggccc cggtgatccg tgacgcctgg tactcgggat
     7741 caccccagta cctgcgcacc atcgcggcca ccggactcga cgggttgggc gagcggtatg
     7801 ccgtgcgcgg gccggtcgtg gtcgcaggcg cgagcctcgg cgggctgacc gcggtgctgc
     7861 tcgggctgct ggcggcaccg cgggtcggcg gggtctactc gcagtccggg tcgttcttcc
     7921 aggtgcgcca cgacctcgac gagagcggct accccttctt cgggcggatc tcacggctcg
     7981 tgcagagcgt gctcgacatg cgcgagaccg agcaccccct cgtcgtcggc atgtcgtgcg
     8041 gggccatcga ggagaacatg gccaacaacc gtgacatggc ggcggccctg cgccgggccg
     8101 ggcacacggt cgagctgacc gaggtcgccg acctgcacaa ctacacggcc tggcgcgacg
     8161 cactcgatcc cggcctgacc cacgtgctgc gcaccgtctg gggcgagacc ggctgatcct
     8221 ctcgcgggca gtgcttgcga gagggggtgg tgaccggcag ggtgtgcgca tggagacgag
     8281 cgccgtgcgg ctgcaggttc ccgggcacga cgtcgacctc gaggtcgtcc gctacggagc
     8341 gtgggggcgg ccggtgctgc tcttcccgtc cgagggtggg tcggcccgag acgcgcaggg
     8401 caacggcatg ctggatgccg tgcgccccct catcgaggca ggtcgggtca gcctgttctg
     8461 cgtcgactcg atggacgggt ggacgtggtc ggccaatgac cagccgaccg aggagcgggc
     8521 ccgccgggcg cagacctaca cggcgtggtt gtcgcagcag gtgctgccgt gggtccagga
     8581 gcaggtcggt gggggacagg agatcatcac cgtcggcgtc tcgctgggcg cctaccacgc
     8641 cgtgcacctg acctttcagc gcgcagacgt cgcaccgatg gccatcggcc tctcgggcaa
     8701 ctacgacccg acgagctgga acggctgggg tgagctcggc gacgcgacgt acttcgcgaa
     8761 ccccacggcg tacgtggcga acatggacgg tgaccacctc gactggatca ggtcgcgtgt
     8821 ctccgtgctg ctcgtcgtcg gcgaaggccc cttcgaggtc tcacccaccc ggtcgctgcc
     8881 ggccacccgg gagttcgcgg ccctgctggc cgacaagggg attcgttacg agctcgacgt
     8941 gtggggccac gacagcgcgc acgactggcc gtggtggcaa cgtcagcttg ctcatcacct
     9001 ccagcgtttc gtgtgacgtg accaccgcgg cccgccgcga gaccgctgtg gtgcgccacg
     9061 gcatccgacc cgcagttcca cccgaggagt aggagaca
//
LOCUS       DAOSOC010000098         9042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7659_length_9042_cov_6.007121, whole genome shotgun sequence.
ACCESSION   DAOSOC010000098 DAOSOC010000000
VERSION     DAOSOC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7659_length_9042_cov_6.007121"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PL014_08420"
     CDS             <1..1293
                     /locus_tag="PL014_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HOT57152.1"
                     /translation="PDAVPAMPTAPVAAKYHQLTLSYTTTPVAATRVEAWSGWRHTVR
                     SSGEFTPFRRDVDALGHTGMVAVGLEVPESVTGSTVSIHLVLDSAAPCGSTAPLTAHW
                     QWWDGATWQALAVSDGTHGLREAGLVRFVAPQGWAAGCSDVTASSGRWIRFVTDQPRR
                     IGVVRDVVTDAVVAEFVSRAPDPQLDPSPATALPPGTIKGTLTPVPGVTKVTNVASVR
                     GRGPEDDPAYATRASALVRHRGRALTPWDYEQTVALAVPEVARLRCLPHTDTDGNRSP
                     GTVGLVVVPDRPENPQPRPSVSLAGRIIDTLRPVMPLTASVAILCPAYVPVSVGASVR
                     LRRGVAALQGRAAVEAALESLLHPTLAAEPRWGLSLYASTVVAVLERLTVVDVVLAFE
                     LRAQLPTGLTPVEVVEVDACRGLYCSTGTHEIACEEQL"
     gene            1290..>2664
                     /locus_tag="PL014_08425"
     CDS             1290..>2664
                     /locus_tag="PL014_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57153.1"
                     /translation="MSTEPITLTEPVAPDADWDTVRGRIVATLVAEAAPGWTDHNAAD
                     PGITLAEAAAFTLADLHHRIATTGLADWPLAWSAWEPLGERHWDHTLPAGDPARLTAL
                     ATALAATADDLEPLVRACASRGEALALVGTAPWATLVPAVDRPALVDVLRGAMVREVA
                     QHRADTVADVLDEAIERSGSAATVAAQDALAVELLGHDLPLWVTERAALVRRERRRRV
                     REAAAARAAQSRAAATSVERAEVVTLLTDDGLSAEEALIASALAPIPADLVPEDLEER
                     DGTSRIWPPHGIQALTCEPVTDLDYARRARTHRGVRRAWAVPGRLDGVAWHGLVTPSA
                     ADLPAGDPAAAWAIDPDAAAVTLVVEAVRRPRDVPTFLREVLRVAIGTECGRPFPPWR
                     DDLDDDQPHRVIGDEVGAALLADVEVQVRATLVIGAGSDGARVLQAAADALETFFDDG
                     RTGVTGLD"
     assembly_gap    2665..2764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2909..3769
                     /locus_tag="PL014_08430"
     CDS             2909..3769
                     /locus_tag="PL014_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57154.1"
                     /translation="MRSPDFTATSALLPPVYQEQAASFAQVDAYLGLADELARAQVEA
                     LEDLLAVTGPDAALRWPTHLPLAAGAEALVANLTATYDEVARWLSFTVPSTWPVGAQG
                     VAARRAFLGKATRLWRRRGTPRGFIDWFTLYHRLSVTTERPVLVEHYKIAGPGFDPDP
                     STATLFVPVSASVATYAQRIGVAQFVRWYAPAHLDIRVCFVRPDVFDTFAVFADPAVL
                     ADTSSAGLATYESDLKAQAKLLRELLCSVVSVVSHADGIHLYGCPAERPPVAERDIDH
                     LGIGALPTDD"
     gene            3789..5807
                     /locus_tag="PL014_08435"
     CDS             3789..5807
                     /locus_tag="PL014_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57155.1"
                     /translation="MTDNEYPEFVKGQTLTSDELNELTTHLRHRDRLVGRMTGFGVNC
                     GLAGSTAGSVITISPGLAVDQRGEPLVLDSARTIDLASATPVGGWSFIDGTKEGWSVI
                     LRATDTGVPHTTCTESSCKGHSTTHTAGVELVLAVGRVAGPRFDFPDDALRSLTPILL
                     SKTSQPLTAYSTLRDQVSGLLKNAPGGPLVDPALITLLDGTSIPSSDLAGVKGYKVAW
                     LNMVLFATMDLLRCRALLATSCQRDTTTPGVVVGHVTRAGSVATFSPRYRHAWEPPTG
                     LTTSLLGGSGESPCAPVKDRLETIISGYAPPTPPPTPPSTGGVIVDPPIRFCPKGKIV
                     MRGECINVPFPGEFFEVPFPPEDYFTIDPGIYINPDPTRYLVDDPSIFYGTEHLNFLD
                     DGVLQAGGLLGYKGTDVVATLTQTIKDLGVEANVSAIAMTAVKDAVGVEPTLSFSPSD
                     EVLVLTDAAGVAVGMARVPAVRSVRDAGTAIPAAKAAVAEVQVLSGTVDRQLKGFEGA
                     LREFDGGLQGLTKEFSTLRDGAFDQSGYGVRIGMLEQQLARTVVYGERISTLEGRITP
                     VKGLATATGVRPELATGLMEFSRSAIEAMRTIDLPDNRALTKSIAVAEKARGELARLV
                     TAGEVDALGPATLQVLDTMRTMVKSAGTDAALGARLDEQVAALEGLIR"
     gene            5804..7060
                     /locus_tag="PL014_08440"
     CDS             5804..7060
                     /locus_tag="PL014_08440"
                     /inference="COORDINATES: protein motif:HMM:NF039901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HOT57156.1"
                     /translation="MSDVVIGTLRLRGPHASRLARVAATTLPAALDAALDGYPDGRVD
                     VVSVPFPVDAGLDDSTLAALWAEAIRAAVHDTLAAGARGRVSTAEPDQVTARSSPTAA
                     DAVRAALAWVAGGARPDAVPRQAWRVADPLLVDECRRLLAGRWGEVVGALARVVSQAG
                     TPVVARAPEDEPDRDGLRGAGGAVTPAEVDATEAGGEGAARPGHPASDPVGDPVTSPG
                     SPGAPGERARAAADLALVSTVGDLVDETSSAVIDTAHLTTAAGLVLLYPWLADHCRSA
                     VALHPGLPAAPVRAAALALLVSAQDPPRDDPLVRLLAGSPGEAGLPLPVSGAVNLRRR
                     DEVEQSAQEVLRNFAALLPGFAGSTADFVRAEWVMRAGLLDERSDHVRLSAAARPLDV
                     VLGALPYPLGLLALPWSRPISVRWQR"
     gene            7057..8412
                     /locus_tag="PL014_08445"
     CDS             7057..8412
                     /locus_tag="PL014_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT57157.1"
                     /translation="MSTRPTVATPVAELGAALEMLDAVIRVRAEERRGGPAGRPLAPS
                     LVEGSGGLEGAAAADEGIPPLERYDGGGPLAATIAMAGLGPAEALVLVAAVAPFVDER
                     FAAAYATLTDRPGVTGLTGEVARTLAARTLAGRLDVTALLSEAGTLRATGLLTLDPPD
                     DLSGTLAPDPPLLAWLLGQPPPPPRVSSGFPARPLTTVHTLADVVLPARARAKVADIE
                     RRIAHRERVVHTWGFGRHHDNVGGLLALLHGPPGTGKSMTAAAIAASAGLTAYVIDLS
                     ALVSKYIGETEKALAAVFERAERERCILVFDEADAIFGQRTEVSDAHDRYANQEVSYL
                     LSRIETHQGVVILTTNLLGNIDPAFQRRIHVMVEFPAPGAPEREQLWRRALPPDLPVA
                     DDLDVVALAERFAITGAEVRDAAVDAAYLAAAGDGVVSQDLLVTAIRRQYEKAGRSAP
                     P"
     gene            8409..>9042
                     /locus_tag="PL014_08450"
     CDS             8409..>9042
                     /locus_tag="PL014_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57158.1"
                     /translation="MTAVASRAPAAAGRAAGSGPEAAERSAAVLGPLGVATERAPVLD
                     RVPGPVSDDPVVVLEGLLAGGLEAVAARWGQGGGSVVLSGRRAFVDVTGSAGFGPDGV
                     LDGRVELRADLQVLGLALGSATWVVHGWEPSAELALPAAEIRGTALRVDLAALPPEVT
                     LTASAGIAATTTDGGVTLELPGDAVPLGEPISPRIAELYRGLLGVDLSSLV"
BASE COUNT         1082 a         3400 c         3275 g         1185 t
ORIGIN      
        1 cccgacgccg tgcccgcgat gccgaccgcg cctgttgccg cgaagtacca ccagctgacc
       61 ctgtcgtaca cgacgacccc ggtcgcggcg acgcgcgtcg aggcgtggtc ggggtggcgg
      121 cacaccgtgc gctcctccgg cgagttcacc ccgttccgcc gtgacgtcga cgccctcggg
      181 cacacgggca tggtggccgt ggggctcgag gtgcccgagt cggtgaccgg gtcgacggtc
      241 tcgatccacc tcgtgctcga ctccgcggcg ccgtgcggct ccaccgcccc gctcaccgca
      301 cactggcagt ggtgggacgg cgccacctgg caggcgctcg ccgtctccga cggcacccac
      361 ggcctgcgcg aggccgggct ggtgcggttc gtcgcgccgc aggggtgggc ggcgggctgc
      421 tccgacgtca cggcatccag tgggcgctgg atccggttcg tcaccgacca accgcgccgc
      481 atcggcgtgg tgcgcgacgt cgtcacggat gccgtggtcg ccgagttcgt gtcgcgggca
      541 ccggacccgc agctcgaccc gagccccgcg accgcactgc cgcccggcac catcaagggc
      601 acgctcaccc ccgtcccggg ggtcaccaag gtcacgaacg tcgcgtcggt gcgcggccgc
      661 gggcccgagg acgacccggc ctacgcgacg agggcgtcgg ccctcgtgcg gcaccgcggc
      721 cgcgcgctca ccccgtggga ctacgagcag acggtggcac tggccgtccc ggaggtcgcc
      781 cggctgcgct gcctgccgca caccgacacc gacggcaacc gctcacccgg cacggtcggc
      841 ctcgttgtcg tgccggaccg gcccgagaac ccccagccgc ggccgagcgt cagcctcgcc
      901 gggcggatca tcgacacgct gcggccggtc atgccgctca ccgcctcggt cgcgatcctc
      961 tgccccgcct acgtgcccgt ctcggtgggg gcctcggtgc ggttgcgtcg tggggtggcc
     1021 gccctgcagg gacgggccgc cgtcgaggcg gcgctcgagt cgttgctgca ccccacgctc
     1081 gccgccgagc cccggtgggg cctgtcgctc tacgcctcga cggtggtggc ggtgctcgag
     1141 cgactgaccg tcgtcgacgt cgtgctcgcc ttcgagctgc gagcgcagct gccgaccggg
     1201 ctgaccccgg tcgaggtggt cgaggtcgac gcgtgccgag ggctctactg ctcgaccgga
     1261 acccacgaga tcgcctgtga ggagcagctg tgagcaccga gccgatcacc ctgaccgaac
     1321 ccgtggcacc ggatgccgac tgggacaccg tgcgtgggcg catcgtcgcc accctcgtcg
     1381 ccgaggccgc gccggggtgg accgaccaca acgccgccga ccccggcatc accctcgccg
     1441 aggcggccgc gttcaccctg gccgacctgc accaccggat cgccacgaca gggctcgccg
     1501 actggcccct cgcgtggtcg gcgtgggaac cactggggga acggcactgg gaccacaccc
     1561 tgccggccgg tgacccggcc cggctcaccg cgctcgccac tgccctggcc gcaaccgcgg
     1621 acgacctcga accgctggtg cgggcctgcg ccagccgggg tgaggccctg gccctcgtcg
     1681 gcaccgcgcc gtgggccacc ctcgtgcccg cggtggaccg acccgccctg gtggacgtgc
     1741 tgcgcggcgc gatggtccgc gaggtcgccc agcaccgcgc ggacacggtg gccgacgtcc
     1801 tcgacgaggc gatcgagcgc agcggcagcg ccgcgaccgt ggctgcgcag gacgccctgg
     1861 ccgtcgagct cctgggccac gacctccccc tgtgggtcac cgagcgcgca gcactggttc
     1921 ggcgcgaacg tcggcgccgc gtgcgcgagg cggccgctgc ccgcgcggcg cagagccgcg
     1981 cggccgccac gtccgtggag cgggccgagg tcgtcacgct gctgaccgac gacgggctga
     2041 gcgccgagga ggcgctcatc gcgagcgccc tcgccccgat ccccgcggac ctggtgcccg
     2101 aggacctcga ggagcgcgac gggacctcga ggatctggcc gccgcacggc atccaggccc
     2161 tgacgtgcga gccggtcacc gacctcgact acgcgcggcg ggcgcgaacc catcgcgggg
     2221 tgcgacgcgc gtgggccgtg ccggggcgcc tcgacggcgt cgcctggcac gggctggtga
     2281 ccccgtcggc cgccgacctg ccggccggtg accccgctgc ggcctgggcg atcgacccgg
     2341 atgccgcggc cgtcaccctc gtcgtggagg cggtgcggcg tccccgcgac gtccccacct
     2401 tcctgcgcga ggtgctgcgc gtcgcgatcg gcaccgagtg cggcaggccc ttcccgccgt
     2461 ggcgggacga cctggacgac gaccagccac accgcgtcat cggcgacgag gtcggtgccg
     2521 cgctgctcgc cgacgtcgag gtgcaggtgc gggcgaccct cgtcatcggg gccggcagcg
     2581 acggggcccg cgtgctgcag gccgccgccg acgcgctcga gacgttcttc gacgacgggc
     2641 gcacgggcgt gaccggcctc gacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnntccgcg acgtccacct gcggctcgcc gacgggggcg cgtggaccac cgccgccgac
     2821 ggggcgctgg ccatccctgc gggcaccgtg ccggtgcggg tcggcaactg cttcaccgtc
     2881 gacatctcgc gcagcggggg gtgcggcgat gcgtagcccc gacttcaccg cgacctcggc
     2941 cctgttgccc cccgtctacc aggagcaggc cgcgtccttc gcgcaggtcg acgcctacct
     3001 cggcctggcc gacgagctcg cccgggcgca ggtcgaggcc ctcgaggacc tcctcgcggt
     3061 gaccggcccg gatgcggcac tgcggtggcc gacccacctg ccgctggcgg ccggtgccga
     3121 ggcgctcgtc gccaacctca ccgcgaccta cgacgaggtg gcccggtggc tgtccttcac
     3181 cgtgccgtcg acgtggccgg tgggtgcgca gggggtggcg gcccgccgcg ccttcctcgg
     3241 caaggccacg cggttgtggc ggcggcgtgg cacgccgcga gggttcatcg actggttcac
     3301 gctctaccac cggttgtccg tcaccaccga gcggccggtg ctcgtcgagc actacaagat
     3361 cgccggaccc ggcttcgacc ccgacccctc gaccgccacg ctgttcgtgc ccgtctcggc
     3421 ctcggtggcg acctacgcgc agcggatcgg ggtcgcccag ttcgtccggt ggtacgcccc
     3481 ggcccacctc gacatccggg tgtgcttcgt gcggcccgac gtcttcgaca ccttcgccgt
     3541 cttcgccgac ccggcggtgc tggccgacac cagcagcgcc gggctcgcca cgtacgagag
     3601 cgacctcaag gcgcaggcga agctgctgcg cgaactgctg tgttcggtgg tgtccgtcgt
     3661 ctcgcacgcc gacggcatcc acctctacgg ctgcccggcc gagcgcccgc cggtcgccga
     3721 acgcgacatc gaccacctgg ggatcggcgc gctccccacc gacgactagg gcggaaggcg
     3781 atcacaccat gacagacaac gagtacccgg agttcgtcaa gggacagacc ctgacctccg
     3841 atgagctgaa cgagctcacc acccacctgc gccaccggga ccggctggtc ggccggatga
     3901 ccgggttcgg cgtcaactgc ggcctcgccg ggagcaccgc gggctcggtc atcaccatct
     3961 cgccggggct ggccgtcgac cagcgcggcg aaccgctcgt gctcgacagc gcccgcacca
     4021 tcgacctcgc ctccgccacg cccgttggtg gctggtcctt catcgacggc accaaggagg
     4081 ggtggagcgt catcctgcgg gccaccgaca cgggggtgcc gcacacgacc tgcaccgagt
     4141 ccagctgcaa ggggcactcg accacgcaca cggccggggt cgaactcgtc ctcgcggtgg
     4201 ggcgggtcgc cggcccccgt ttcgacttcc ccgacgacgc gctgcgctcg ctcaccccga
     4261 tcctgctgtc gaagacgtcg cagccgctca cggcctactc gacgttgcgc gaccaggtca
     4321 gtggcctgct gaagaacgcg ccgggcgggc cgctggtcga cccggcgctc atcaccctgc
     4381 tcgacggcac gagcatcccg agctccgacc tcgccggggt caagggctac aaggtcgcgt
     4441 ggctgaacat ggtgctcttc gcgaccatgg acctgctgcg ctgccgcgcc ctgctcgcca
     4501 cgtcgtgcca gcgcgacacg acgacacccg gggtggtcgt cggccacgtg acccgggcag
     4561 gcagcgtcgc gaccttcagc ccccgctacc ggcacgcctg ggaaccgccg acgggcctca
     4621 cgaccagcct gctcggtggt tcgggtgagt cgccctgcgc cccggtcaag gaccggctcg
     4681 agacgatcat ctcgggctac gcgcccccga ccccgccgcc caccccgccg tcgacggggg
     4741 gcgtcatcgt cgacccgccc atccggttct gcccgaaggg caagatcgtc atgaggggcg
     4801 agtgcatcaa cgtgcccttc cccggggagt tcttcgaggt cccgttcccg ccggaggact
     4861 acttcaccat cgatcccggg atctacatca accccgaccc gacgcgctac ctcgtggacg
     4921 acccgtccat cttctacggc accgagcacc tgaacttcct cgatgacggt gtgctccagg
     4981 cgggcggtct gctcggctac aagggcaccg acgtcgtggc gaccctcacc cagacgatca
     5041 aggacctcgg ggtcgaggcg aacgtctcgg cgatcgcgat gacggcggtc aaggatgccg
     5101 tgggggtcga accgacgttg tcgttcagcc cgtccgatga ggtgctcgtg ctcacggacg
     5161 cggccggcgt ggccgtcggg atggcccggg ttccggccgt gcgctcggtg cgcgacgcgg
     5221 gcacggccat cccggcagcg aaggcggccg tggccgaggt gcaggtgctg agcggcacgg
     5281 tcgaccgtca gctcaagggc ttcgagggag cgctacggga gttcgacgga ggcctgcagg
     5341 ggctcaccaa ggagttcagc acgctgcgcg acggtgcctt cgaccagagc ggctacgggg
     5401 tgcgcatcgg gatgctcgag cagcagctgg ctcgaacggt ggtctacggc gaacgcatct
     5461 ccaccctcga ggggcggatc accccggtca aggggctcgc cacggccacc ggggtgcgac
     5521 ccgagctggc cacggggctc atggagttct cccgctcggc catcgaggcg atgcgcacca
     5581 tcgacctgcc ggacaaccgt gccctcacca agtcgatcgc ggtggccgag aaggcccggg
     5641 gcgaactcgc ccggctcgtc accgccggcg aggtcgacgc gctcggcccg gccaccctcc
     5701 aggtgctcga cacgatgcgc acgatggtga agtcggccgg caccgacgcg gccctggggg
     5761 cccggctcga cgagcaggtc gccgcgctgg aggggctcat ccggtgagcg acgtcgtgat
     5821 cgggacgctg cggctgcgtg gaccgcacgc gagccggctg gcccgggtcg cggcgacgac
     5881 gctgcccgca gcactcgacg cggcgctcga cggctacccc gacggccggg tggacgtggt
     5941 ctcggtgccc ttcccggtgg acgccggtct cgacgacagc acgctcgcgg cgttgtgggc
     6001 ggaggcgatc cgggccgcgg tgcatgacac gctcgccgcg ggcgcgcggg gtcgggtctc
     6061 gacggcggag cccgaccagg tcaccgcccg gagctcgccg accgcggcgg atgccgttcg
     6121 cgcggccctg gcgtgggtgg cgggtggtgc ccgaccggat gccgtgcccc ggcaggcgtg
     6181 gagggtcgcc gacccgctcc tcgtcgacga gtgccgtcgg ctgctggccg gccgctgggg
     6241 cgaggtggtc ggcgccttgg ctcgtgtggt gagtcaggcc ggcacgccgg tggtggctcg
     6301 cgcccccgag gacgagccgg accgggacgg tctgcgcggt gcggggggtg cggtcacccc
     6361 ggcggaggtg gacgcgaccg aagcgggcgg tgagggtgca gcgcgacccg ggcacccggc
     6421 atccgatccc gtcggggacc ccgtgacgtc gccgggctcg ccgggggccc cgggggagcg
     6481 ggcacgtgcg gcggccgacc tcgcgctggt gtcgaccgtg ggcgacctcg tcgatgagac
     6541 cagttcggcg gtgatcgaca ccgcgcacct gacgaccgcc gcagggctgg tgctgctcta
     6601 tccgtggctg gccgatcact gccggtccgc cgtggcgctg cacccgggcc tgcctgctgc
     6661 cccggtgcgc gccgcggcgc tggcgctgct cgtcagcgcg caggacccgc cgcgcgacga
     6721 cccgctcgtg cgcctcctcg ccgggagccc aggggaggcc gggctccccc tgccggtctc
     6781 gggcgcggtg aacctgcgac gccgcgacga ggtcgagcag tcggcgcagg aggtgctgcg
     6841 gaacttcgcc gcgctgctgc ccgggttcgc ggggtcgacg gccgacttcg tgcgagctga
     6901 gtgggtgatg cgcgccgggc tgctcgacga gcgctccgac cacgtgcggc tgagcgcggc
     6961 ggcgcgaccg ctcgacgtcg tgctgggcgc gctgccctac cccctcgggc tgctggcgct
     7021 gccgtggtcg cgcccgatca gcgtgcggtg gcagcggtga gcacccgccc gacggtcgcc
     7081 accccggtgg ccgaactcgg tgcagcgctg gagatgctcg atgccgtcat ccgggtccgg
     7141 gccgaggagc gcaggggtgg cccggccggg cgcccgctgg cgccctcgct ggtcgagggt
     7201 agcggcgggc tggagggggc agcggctgcg gacgagggca tccccccgct cgagcggtac
     7261 gacggcggtg gcccgctcgc cgcgacgatc gccatggccg ggctcgggcc ggccgaggcc
     7321 ctcgtgctgg tcgcggcggt ggccccgttc gtcgacgagc gcttcgcggc cgcgtacgcc
     7381 acgctcaccg accggcccgg ggtcaccggc ctcaccggcg aggtcgcccg caccctcgcg
     7441 gcccgcaccc tggcgggccg gctcgacgtg accgccctgc tcagcgaggc cggcaccctg
     7501 cgcgccaccg gcctgctcac cctcgaccca cccgacgacc tcagcggcac gctggcgccc
     7561 gacccgcctc tgctcgcctg gctgctcggg cagccgccac caccgccacg ggtgtcctcg
     7621 gggttcccgg cccgcccgct cacgacggtg cacaccctgg ccgatgtcgt cctgccggca
     7681 cgggcccgcg ccaaggtcgc cgacatcgag cgacggatcg cccaccgcga acgcgtcgtg
     7741 cacacctggg gtttcgggcg gcaccacgac aacgtcggcg ggttgctcgc cctgctgcac
     7801 gggccgccgg gcacgggcaa gtccatgacg gccgctgcga tcgcggcctc ggcgggcctg
     7861 accgcctacg tcatcgacct gtcggcgctg gtgtcgaagt acatcggcga gaccgagaag
     7921 gccctggcgg ccgtcttcga gcgggccgag cgtgagcgct gcatcctcgt cttcgacgag
     7981 gccgacgcga tcttcggcca gcgcaccgag gtctccgacg cccacgaccg ctacgcgaac
     8041 caggaggtct cctacctgct ctcgcggatc gagacccacc agggggtcgt catcctcacg
     8101 accaacctgc tcggcaacat cgaccccgcg ttccagcgcc gcatccacgt gatggtcgag
     8161 ttccctgcgc ccggcgcccc cgagcgtgag cagctctggc ggcgggccct gcctcccgac
     8221 ctgcccgtcg ccgacgacct cgacgtcgtg gcgctcgcgg agcgcttcgc gatcaccggg
     8281 gccgaggtgc gcgacgccgc cgtggatgcc gcgtacctcg ccgcggccgg cgacggggtc
     8341 gtcagccagg accttctcgt gacggcgatc cgtcggcagt acgagaaggc gggtcgatcg
     8401 gccccaccgt gacggccgtc gcgagccgcg cgccggcggc tgcggggcgg gccgcggggt
     8461 ccgggcccga ggccgccgag cgctcggcag ccgtgctcgg gccgctcggc gtggctaccg
     8521 agcgcgcacc ggtgctcgac cgcgtcccgg gtccggtgtc cgacgatccc gtcgtggtgc
     8581 tcgagggcct gctcgccggg gggctcgagg cggtcgcggc ccggtggggg cagggcggag
     8641 gttcggtggt gctctcgggg cggcgggcct tcgtcgacgt caccggcagc gccggattcg
     8701 gccccgacgg ggtgctcgac ggccgggtcg agctgcgcgc cgacctccag gtgctggggc
     8761 ttgcgctcgg ctccgccacg tgggtggtgc acgggtggga gccctcggcg gagctcgccc
     8821 tgcccgcggc cgagatccgc ggcacggcgc tgcgcgtcga cctggcggcg ctgccgcccg
     8881 aggtgacgct cacggcatcc gcgggcatcg ccgcgaccac gaccgacggc ggcgtgacgc
     8941 tggagctccc gggcgacgcc gtcccgctcg gtgagccgat cagcccacgc atcgccgagc
     9001 tctaccgggg cctgctcggg gtcgacctgt cgtcgctcgt cg
//
LOCUS       DAOSOC010000099         9000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7705_length_9000_cov_9.738849, whole genome shotgun sequence.
ACCESSION   DAOSOC010000099 DAOSOC010000000
VERSION     DAOSOC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7705_length_9000_cov_9.738849"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PL014_08455"
     CDS             <1..605
                     /locus_tag="PL014_08455"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT57159.1"
                     /translation="PPPPVDAPPPPPPAPPVGSYPPPPAPYPPSATGGYPVPPPAPFP
                     TSSSSAGQPADLLPRFLAKLVDGVVMFFINIVVGLFVAFGLNSGFIRSAIATLISTAI
                     TLAYYAFLESSRGQTVGKMVVGLKVQNLAGQNPTMEQALKRNIYVALGFLGILPVVGF
                     FGSLAELAAVIFIAVTINSDTPLFRGWHDKFADTQVIKTR"
     gene            complement(592..1212)
                     /locus_tag="PL014_08460"
     CDS             complement(592..1212)
                     /locus_tag="PL014_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57160.1"
                     /translation="MGERQQFRGRIAGVGSTSGIRVVVGWWQESPYGTFADAMVERAD
                     GHRMLLAPTEPVADLVSTTYTFDEVRVEPVAVTDQVRPGRWEFTSPSLTLTLEVGARM
                     PLGHLLRLVPAAVSASPAFATAVDPVARVVLRGVRTRGQARAGRREYYGARDMHAVTS
                     VRGVLDGIPLGDLGPVDPPCRFGFSSTPRAPSVTDVVTTIGHVSGS"
     gene            1243..2553
                     /locus_tag="PL014_08465"
     CDS             1243..2553
                     /locus_tag="PL014_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT57161.1"
                     /translation="MAATLTPTARRGYGLGSVATGSFGTVPGLLLLPYLTDRLGIAAG
                     LAGLIVFLPKAWDVVLNPIAGRISDRSTHPGGRRRPFLIWSGTALAVAFVLLFSGPTS
                     PSGVAATWVVVTFLVCASAYAFFQVPYVAMPAEMTEDYDERTRLMTWRVAILALAILV
                     SGGLSPIIRDALGPEWGYRGVGLFVGALILLGTVGVWRGTRDTPMSRAATAGGSLADQ
                     LRVVAASPPFRTLLLVFVLQALATGAMLAGVDYVARVLLGGGAASTVLFLCFVAPALL
                     VTPLWQRVGEARGKRAGYLWGSVLLLVGAAATLATVRVGLAAAALSVAVVGIGYAACQ
                     MFPLAMLPDVAAADTAATGEDRAGTYTGVWTAGETLGLAIGPFLYAAVLTIGGYVSST
                     DAGARQPDSAQVAIALGFTVMPAVLVAISLVFLRRYRLDVEVAR"
     assembly_gap    2555..2654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2660..4075
                     /locus_tag="PL014_08470"
     CDS             2660..4075
                     /locus_tag="PL014_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT57162.1"
                     /translation="MPTDEVLATLRMLQGLDLPAHGGRTLAYVYDSGLADADAVGLEA
                     LAMFAGSNGLDPTAFPSLLKMENDLVALAGGLLDAPDGFAGSATSGGTESILLAVLAA
                     RKGSDHPSPSMVLPTSAHAAFLKAAAYLGVRAVLVDVDPVTLRADPAAMAAVVDDSTV
                     LVVASAPSYAHGVIDPVPEIAALAAARGIRCHVDACIGGWVLPHLDTATPWTFAVEGV
                     TSVSVDLHKYAYTPKGVSVLLHRTAALRSTHLFASANWPGYTMLNATMQSTRSGGPLA
                     AAWAVTRRIGLDGYAELARRAREATLAIARAADGIPGLRVLVTPDSTLLALATDDSCD
                     AFTVADEMLERGWYVQPQLSFGNLPPTLHLTLSAATAPAVDELVDDLSDAVAAARAAG
                     PVRVDPGLAALLGGIDPAALDDAGFAGILGAAGLAGADGSLALPSRMAGVNALLDACP
                     PAVREALLLGVLDRLSRPTPG"
     gene            complement(4091..5053)
                     /locus_tag="PL014_08475"
     CDS             complement(4091..5053)
                     /locus_tag="PL014_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOT57163.1"
                     /translation="MNTTRRHVSLRFFVAAALAAPLLLLAAPAPDAVGATRTCSSTTA
                     VANRPTLRYGDTGSCVKVLQNILLGKGYSIGSSYATGTFGSGTLGAVRRYQSTKLTLV
                     IDGVVGPTTWNRLVNGGGTTYSISSGPNTSARVILSFDDCPKSYSAFQSAVLGAKSLG
                     VALVLAPTGNCISAGRFSPSYARANGHYVINHSISHPNLSNLSYSSVRYQLGSPGVVT
                     SYGRPPYGSYDFTVRDAYASKGMRIWTWDVDTSDYTGRTQAQVVNHVVTYSRAGDTVL
                     MHMGWNAFNKSAIAAMKSGLAAKGLGVCRNPGTTTPTYPKTIGC"
     gene            5243..>5655
                     /locus_tag="PL014_08480"
     CDS             5243..>5655
                     /locus_tag="PL014_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57164.1"
                     /translation="MQLRELILLLLLVLVVAAVIAVVVRRGGSKREGGPHSQTEHVGS
                     QAEWLDAGDRAEPLRAAQAPAAVALGASAAAESAADVEPAVPSEPSAEADTAVDTLDD
                     AETAADAVALEDGGAAPAVADDVAMTRAERREAREA"
     assembly_gap    5656..5755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5756..5991
                     /locus_tag="PL014_08485"
     CDS             <5756..5991
                     /locus_tag="PL014_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57165.1"
                     /translation="TGRRISGFLELRDGGFGVGSAAPIEDGAQPLDHPVQAYRDTMTF
                     REPGVPGYDSAEPDVWFYDASAAERSGFRPADG"
     gene            6074..7624
                     /locus_tag="PL014_08490"
     CDS             6074..7624
                     /locus_tag="PL014_08490"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOT57166.1"
                     /translation="MRIGVPRETRPGETRVAATPKTVEQIIALGYAVFVETGAGAASS
                     FDDAAYARAGADVIDDAVWNADVLLKINAPSDAEIARLRPGQVVVSLLSPALNPGLVD
                     ALAARGVTALAMDAVPRISRAQSLDVLSSMANIGGYRAVIEAAHEFGSFFTGQVTAAG
                     KVPPAKVLVVGAGVAGLAAIGTANSLGAIVRAFDARPEVGEQVESMGAEFLRIDVEET
                     GPSADGYAKETSADFDAKAAALYAEQAADVDIVITTALIPGRPAPRLLTEAMVASMKP
                     GSVIVDMAAANGGNVAGSVADEKVVTANGVTILGYTDLPGRLPTQASQLFGTNLVNLL
                     KLLTPEKDGELTLDLDDVVQRGMTVVREGEVLWPPPPVQVSAAPASTDVVPATAAPVK
                     AVKEPMSANRRLGLMGVGAAFFLLVASVAPQPFLGHFMVFMLSVVIGFYVIGNVHHAL
                     HTPLMSVTNAISGIIVVGAVLQIGHSSAVVSVLAFVAILVASINVFGGFRVTARMLEM
                     FKREEPAR"
     gene            7621..>9000
                     /gene="pntB"
                     /locus_tag="PL014_08495"
     CDS             7621..>9000
                     /gene="pntB"
                     /locus_tag="PL014_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HOT57167.1"
                     /translation="MSLPVTLVQGAYIVAGLSFILALAGLSRHETARAGNRFGIIGMT
                     IALVATILAATAGTSVSAGRVEVSTEGSGGFGLALILAAMGIGAVIGLDRAKKVEMTG
                     MPELIAMLHSFVGLAAVLVGWNSSVEDTPYPVIHDVEVAIGVFIGAVTFTGSIVAFLK
                     LSARIKSAPLMLPGRNWINLGAIAAFFVLTVVYVVMPADETMARRAVLVLITAVALAL
                     GWHLVASIGGGDMPVVVSMLNSYSGWAAAAAGFLLGNDLLIITGALVGSSGAYLSYIM
                     CRAMNRSFISVIAGGFGVEAGTVAADVDYGEHREITAEGAAAMLTDARSVVITPGYGM
                     AVAQAQYPVADLTRKLRERGIDVKFAIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEV
                     NDDLKDVDVVLVIGANDTVNPAASEDPTSPIAGMPVVRVWEAANVIVFKRSMATGYAG
                     VQNPLFFKEN"
BASE COUNT         1153 a         3103 c         3118 g         1426 t
ORIGIN      
        1 cgccgcctcc tccggtggat gcgccgccgc ccccgccgcc ggcacccccg gtcgggtcgt
       61 acccaccgcc gcctgcgccc taccccccga gcgcgaccgg tggctacccg gtgcccccgc
      121 ccgcaccgtt ccccacttcg tcctcgagtg ccggacagcc ggcggacctc ttgccgcgct
      181 tcctcgccaa gctcgtcgac ggtgtggtga tgttcttcat caacatcgtc gtcggcctct
      241 tcgtggcctt cggactgaac agcggcttca tcaggagtgc catcgcgacg ctgatctcca
      301 ctgccatcac gctggcctac tacgccttcc tcgagtcatc gcgcggccag accgtcggca
      361 agatggtggt gggcttgaag gtgcagaacc tcgctggtca gaacccgacg atggagcagg
      421 cgctcaagcg caacatctac gtcgcgctcg gtttcctcgg catcctcccg gtcgtgggct
      481 tcttcggctc gctggcggag ttggcagccg tcatcttcat cgcggtcacg atcaacagcg
      541 acaccccgct gttccgcggc tggcacgaca agttcgctga cacgcaggtc atcaagaccc
      601 gctgacgtgc ccgatcgtcg tcacgacgtc ggtcaccgac ggagcgcgcg gggtcgagga
      661 gaacccgaag cgacacggcg ggtccacggg ccccaggtcg ccgaggggga tgccgtcgag
      721 cacgccccgc accgacgtca ccgcgtgcat gtcgcgcgcc ccgtagtact cgcgcctgcc
      781 cgcgcgggcc tgcccacggg tgcgcactcc tcgcaggacc accctggcga cggggtcgac
      841 agcggtcgcg aaggcggggc tggccgacac ggcagccggc acgagacgca gcaggtgacc
      901 cagcggcatc cgagcgccga cctcgagggt cagggtcagc gacgggctcg tgaactccca
      961 gcgtccaggt cggacctggt ccgtgacggc gaccggctcg acccgcacct cgtcgaaggt
     1021 gtaggtcgtc gacaccaggt cggcgaccgg ctcggtgggt gccagcagca tccggtggcc
     1081 gtcagcccgc tcgaccatcg cgtcggcgaa ggtgccatag gggctctcct gccaccaccc
     1141 cacgacgacg cggatgccgg acgtcgaccc gaccccggcg atccgcccac gaaactgctg
     1201 gcgctcaccc atgggagcca tcatcgccac tagggtccga gcatggcagc gacgctcacc
     1261 ccgacggcgc gccgcggcta cggtctgggc tcggtcgcca cggggtcgtt cggcacggtg
     1321 cccggcctgc tcctgctgcc ctacctcacc gaccggctgg ggatcgccgc cggcctggcc
     1381 gggctcatcg tcttcctgcc caaggcctgg gacgtcgtcc tcaacccgat cgccggccgg
     1441 atcagcgacc ggtccacgca ccccggaggt cggcggcgcc cgttcctcat ctggtcgggc
     1501 acggccctgg ccgtagcgtt cgtgctgctc ttctccgggc cgacctcgcc gagcggggtg
     1561 gccgcgacct gggtggtcgt gaccttcctg gtctgcgcca gcgcctacgc cttcttccag
     1621 gtgccctacg tcgcgatgcc ggccgagatg accgaggact acgacgagcg cactcgcctg
     1681 atgacgtggc gggtggccat cctggccctg gccatcctcg tcagtggcgg gttgtcgccg
     1741 atcatccgtg acgcgctcgg tccggagtgg ggctaccgcg gggtgggcct gttcgtcggg
     1801 gcgctgatcc tgctcggcac cgtcggggtc tggcgcggca cccgggacac cccgatgtcc
     1861 cgtgcggcga ccgccggcgg gagcctggcc gaccagctgc gggtggtcgc ggcatccccg
     1921 ccgtttcgca cgctgctgct cgtcttcgtg ctccaggcgc tcgcgaccgg ggccatgctg
     1981 gcaggggtcg actacgtcgc ccgcgtgctg ctcggtgggg gagcggcctc gaccgtgctg
     2041 ttcctgtgct tcgtcgcgcc ggccctgctc gtgacgccgc tgtggcagcg ggtcggtgag
     2101 gcccgcggca agcgcgcggg gtacctgtgg ggctcggtcc tgctcctcgt cggggccgcg
     2161 gcgacgctgg ccacggtgcg cgtggggttg gccgcggcgg cgctcagcgt ggccgtcgtc
     2221 ggcatcgggt acgccgcgtg ccagatgttc ccgctcgcca tgctgcccga cgtcgcagcg
     2281 gccgacacgg cggcgacggg ggaggaccgc gccgggacgt acaccggtgt ctggacggcc
     2341 ggggagacgc tcggcctcgc gatcggcccg ttcctgtatg ccgccgtgct caccatcggc
     2401 ggctacgtgt cgtcgacgga tgccggtgcg cgccaacctg attcggcgca ggtcgcgatc
     2461 gcgctcgggt tcaccgtgat gccggccgtg ctcgtcgcga tctccttggt gttcctgcgc
     2521 aggtaccgac tcgacgtgga ggtggcccgg tgacnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnccgcga tgccgaccga cgaggtgctc gccaccttgc ggatgctcca
     2701 ggggctcgac ctgcccgctc acgggggccg caccctggcc tacgtctatg actcgggtct
     2761 cgccgacgcc gatgcggtcg gcctcgaggc acttgccatg ttcgccggct cgaacgggct
     2821 cgacccgacc gcgttcccgt cgttgctgaa gatggagaac gacctcgtcg ccctcgccgg
     2881 tggcctgctc gacgcccccg acgggtttgc cggctcggcc acctccggtg gcaccgagtc
     2941 gatcctgctc gccgtgctgg ccgcccgaaa gggcagcgac cacccgtccc cgagcatggt
     3001 gctgcccacg tcggctcacg ccgcgttcct caaggccgcg gcctacctcg gggtgcgggc
     3061 cgttctcgtc gacgtcgacc cggtcaccct gcgggccgac ccggccgcca tggccgcggt
     3121 cgtcgacgac tccacggtgc tcgtcgtcgc ctcggccccg tcctacgcgc acggcgtcat
     3181 cgaccccgtc cccgagatcg cggcgctggc ggcggcgcgc ggcatccgtt gtcacgtcga
     3241 cgcctgcatc ggcgggtggg tgctgccgca cctcgacact gcgacgccgt ggaccttcgc
     3301 ggtcgagggc gtcacgagcg tgagcgtcga cctgcacaag tacgcctaca cgcccaaggg
     3361 ggtctcggtg ctgctgcacc gcaccgcggc gctgcgcagc acccacctgt tcgcctcggc
     3421 gaactggccc ggctacacga tgctcaacgc caccatgcag tcgacccgct cgggtggccc
     3481 gctggcggcg gcctgggccg tgacccgtcg gatcggcctc gacggttacg ccgagctcgc
     3541 ccggcgggcg cgcgaggcga cgctcgcgat cgcgagggcg gcggacggca tccccggtct
     3601 gcgggtgctc gtcacccccg actcgaccct gctggcgctc gcgaccgacg actcgtgcga
     3661 tgccttcacc gtcgccgacg agatgctcga gcgtgggtgg tacgtgcagc cgcagctgtc
     3721 gttcgggaac ctgccaccga ccctgcacct caccctctcg gcagcgacgg cgcccgcggt
     3781 cgacgagctg gtcgacgatc tctcggatgc cgtcgcggcg gctcgcgcgg cgggcccggt
     3841 tcgcgtcgac cccgggttgg cggccctgct cggcggcatc gacccggcgg ccctggacga
     3901 cgctggcttt gccggcatcc tcggggcggc gggcctcgcg ggcgcggacg gctcgctggc
     3961 gctgccgtcg cggatggccg gtgtcaacgc gctgctcgac gcctgcccgc ccgcggttcg
     4021 cgaggccctg ctgctcgggg tgctcgaccg gctgtcccgc ccgacgccgg gctgagctgg
     4081 ctcggctggc tcagcagccg atggtcttcg gatacgtcgg cgtcgtcgtg ccggggttgc
     4141 ggcacacgcc gagccccttg gcggccaacc cggacttcat ggccgcgatg gcgctcttgt
     4201 tgaaggcgtt ccagcccatg tgcatgagca ccgtgtcgcc cgcccgcgag tacgtgacga
     4261 cgtggttcac gacctgggcc tgggtgcggc ccgtgtagtc actggtgtcg acgtcccacg
     4321 tccagatgcg catgcccttg ctggcgtagg cgtcacgcac cgtgaagtcg tacgagccgt
     4381 agggcgggcg tccgtacgag gtgacgaccc cgggagaccc cagctggtag cgcacgctcg
     4441 agtacgacag gttgctcagg tttgggtggc tgatggagtg gttgatgacg tagtggccgt
     4501 tggcccgggc atagctcggg ctgaaccggc cggcggagat gcagttgccg gtgggagcga
     4561 ggaccagtgc gacgccgagc gacttcgccc ccaggacggc cgactggaac gccgagtagc
     4621 tcttcgggca gtcgtcaaag ctgaggatga cccgcgcgct ggtgttcggt ccggaggaga
     4681 tgctgtacgt ggtgccccct ccgttgacga gccggttcca ggtcgtcggg cccaccacac
     4741 cgtcgatcac gagcgtgagc ttggtggact ggtagcgacg gaccgcgccc agggtcccgg
     4801 agccgaaggt gcccgtggca tagctcgacc cgatgctgta ccccttgccg agcaggatgt
     4861 tctgcagcac cttcacgcag ctccccgtgt cgccgtagcg cagggtcggc cggttcgcca
     4921 cggccgtcgt cgaggagcag gtgcgggtgg ccccgacggc gtcgggcgcg ggagcggcca
     4981 acagcagcag gggtgccgcg agggcggcgg ccacgaagaa cctcagggag acgtgtcgtc
     5041 gggtggtgtt catgatgtcc tccgtaccgg gggacgaagg cgggcccccc gccatcttca
     5101 ccgtactccg acagggcccc cgcgaccaga gaccagcgat cacgtgaacc gggtcgggct
     5161 gcgctgacta cgccgtggaa gcgatgccgc agtaggttga tgggcgagat cgcctccggg
     5221 cagcgtcaac gacaggaatt ccatgcagct ccgcgaactg atcctcttgc ttctgctcgt
     5281 cctggtggtg gctgcggtca tcgcggtcgt ggtgcgccgg ggcggctcca agcgggaggg
     5341 tggaccccac tcccagaccg agcacgtggg ctcgcaggcc gagtggctgg atgcgggcga
     5401 ccgggcggaa ccacttcgtg ctgcgcaggc tcctgcagcg gtggcccttg gcgcctctgc
     5461 cgctgctgag tctgccgctg acgttgagcc ggcggtgccc tccgagccct cggctgaggc
     5521 cgacacagcc gttgacaccc tggacgacgc ggagacggct gcggatgccg tggctctgga
     5581 ggatggcgga gcagcaccgg ccgtggccga tgacgtggcc atgacccggg ccgaacgccg
     5641 cgaggcccgt gaggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgacc
     5761 ggtcgccgca tctccggctt cctcgagctg cgcgacggcg gcttcggagt cgggtccgcg
     5821 gccccgatcg aggacggtgc ccagccgctc gaccacccgg tgcaggccta ccgcgacacg
     5881 atgaccttcc gcgagccggg tgtcccgggc tacgactcgg ccgagccgga cgtctggttc
     5941 tacgacgcgt ccgctgccga gcggtccgga ttccgccccg cggacggctg acgtccgcct
     6001 cccggccgcc tggcaccccc gtagcggcca cccgataggt tcggtacaga tcccgtaggc
     6061 cgagaggaat gtcgtgcgca tcggagtgcc ccgtgagacc cgtcccggtg agacgcgcgt
     6121 ggccgcgaca ccgaagaccg tcgagcagat catcgcgctc gggtacgcgg tgttcgtcga
     6181 gaccggggcc ggtgcggcgt cgagcttcga cgatgccgcc tacgcgcgcg cgggtgcgga
     6241 cgtcatcgac gacgccgtct ggaacgccga cgtcctgctg aagatcaacg ccccctcgga
     6301 tgccgagatc gcccggttgc gcccgggtca ggtggtcgtg tcgctgctct cgccggcgtt
     6361 gaaccccggc ctcgtcgacg cgctcgccgc gcgtggggtg acggcgctgg cgatggatgc
     6421 cgtgccgcgc atctcgcggg cgcagtcgct cgacgtgttg tcgtcgatgg cgaacatcgg
     6481 tgggtaccgc gcagtgatcg aggcggcgca cgagttcggg tcgttcttca ccggtcaggt
     6541 cacggcggcg ggcaaggtgc cgccggccaa ggtcctcgtc gtcggggccg gcgtggcggg
     6601 gctggcagcg atcggcacgg cgaactccct cggggcgatc gtgcgcgcgt tcgacgcccg
     6661 gcccgaggtg ggtgagcagg tcgagtcgat gggcgcggag ttcctgcgca tcgacgtcga
     6721 ggagaccggg ccgtcggccg acggctacgc caaggagacc agtgcagact tcgacgccaa
     6781 ggctgcggcg ctgtacgccg agcaggctgc cgacgtcgac atcgtcatca ccacggccct
     6841 gatcccgggt cgcccggcac ctcggttgct gaccgaggcc atggtggcgt cgatgaagcc
     6901 gggttcggtg atcgtcgaca tggccgccgc caacgggggc aacgtcgcag ggtcggttgc
     6961 cgacgagaag gtcgtcaccg cgaacggggt gaccatcctg ggctacaccg acctgccggg
     7021 gcggctgccc acgcaggcgt cacagctgtt cggcacgaac ctggtgaacc tgttgaagct
     7081 gctcacacct gagaaggacg gtgagctgac cctcgacctc gacgacgtcg tgcagcgggg
     7141 gatgacggtg gtgcgtgagg gggaggtgtt gtggcccccg ccgccggtgc aggtgtcggc
     7201 ggccccggca tccaccgacg tcgtgccggc cacggccgcg cctgtcaagg cggtcaagga
     7261 gccgatgagc gcgaaccggc gcctcggtct catgggcgtc ggcgcggcgt tcttcctgct
     7321 cgtcgcgtcg gtggcgcccc agccgttcct cggccacttc atggtgttca tgctctcggt
     7381 cgtcatcggg ttctacgtca tcgggaacgt ccaccacgcc ctgcacaccc cgctgatgtc
     7441 cgtgacgaac gcgatcagcg gcatcatcgt ggtcggggcg gtcctgcaga tcggtcattc
     7501 cagtgctgtg gtctcggtgc tcgcgttcgt cgccatcctc gtggcgagca tcaacgtctt
     7561 cggcgggttc cgggtgacgg cgcgaatgct cgagatgttc aagcgagagg agccggcgcg
     7621 atgagccttc cggtgacact cgtccagggc gcgtacatcg tcgctggcct gtccttcatc
     7681 ctcgccctcg caggcctgtc ccggcacgag accgcgcggg caggaaaccg cttcggcatc
     7741 atcggcatga ccatcgccct cgtcgccacg atcctggcgg ccactgccgg aacgtcggtg
     7801 tccgcgggtc gtgtcgaggt cagcaccgag ggatcgggcg ggttcggcct ggcgctgatc
     7861 ctcgcagcaa tgggcatcgg cgcggtgatc gggctcgacc gggccaagaa ggtcgagatg
     7921 accggcatgc ccgagctcat cgcgatgctg cacagctttg tcgggctcgc ggccgtgcta
     7981 gtgggctgga actcctccgt cgaggacact ccctacccgg tgatccacga cgtcgaggtc
     8041 gcgatcgggg tcttcatcgg cgcggtgacc ttcacggggt cgatcgtcgc gttcctcaag
     8101 ttgtcggcgc ggatcaagtc ggccccgctg atgctgcccg ggcgcaactg gatcaacctg
     8161 ggcgcgatcg cggcgttctt cgtgctcacc gtggtctacg tggtgatgcc ggccgacgag
     8221 accatggccc gcagggccgt gctggtgctc atcaccgccg tcgccctcgc gctcggctgg
     8281 cacctggtcg cctcgatcgg tggcggcgac atgccggtcg tggtgtcgat gctcaacagc
     8341 tactcggggt gggcggcggc cgcggccggg ttcctgctcg gcaacgacct gctcatcatc
     8401 accggcgcgc tcgtgggctc ctcgggtgcc tacctgtcgt acatcatgtg ccgggcgatg
     8461 aaccggtcgt tcatctcggt catcgcgggg gggttcggcg tcgaggccgg caccgtggca
     8521 gctgacgtcg actacggcga gcaccgcgag atcaccgccg agggtgccgc cgcgatgctg
     8581 accgatgccc gcagcgtggt gatcacgccc gggtacggca tggccgtcgc ccaggcgcag
     8641 tacccggtgg ccgacctgac ccgaaagctg cgcgagcgcg gcatcgacgt gaagttcgcg
     8701 atccacccgg tcgcgggtcg actgcccggg cacatgaacg tcctgctcgc cgaggcgaag
     8761 gtcccctacg acatcgtcct ggagatggac gaggtcaacg acgacctcaa ggacgtcgac
     8821 gtcgtgctcg tcatcggcgc caacgacacg gtcaaccccg ctgcctccga ggaccccacc
     8881 tcaccgattg ccgggatgcc ggtcgtgcgg gtgtgggagg cggcgaacgt catcgtgttc
     8941 aagcgctcca tggccacggg ctacgccggc gtgcagaacc cgctcttctt caaggagaac
//
LOCUS       DAOSOC010000100         8967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7747_length_8967_cov_7.633303, whole genome shotgun sequence.
ACCESSION   DAOSOC010000100 DAOSOC010000000
VERSION     DAOSOC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7747_length_8967_cov_7.633303"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1989)
                     /locus_tag="PL014_08500"
     CDS             complement(157..1989)
                     /locus_tag="PL014_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOT57168.1"
                     /translation="MTHRAADAATERDVDVDVVVVGSGFGGSVTALRLAEKGYRVLVL
                     EAGQRFEDEDFAETSWDVRRYLWAPQVGCFGIQRIHRLPDVVVLAGAGVGGGSLNYAN
                     TLYVPPRPFFEDEQWSDITDWQAELAPHYDTASAMLGVVTNPCEGVVEQLMRRTADDL
                     GVGDTFRKTPVGVYFGRPGETVDDPYFGGAGPRRTGCTECGNCMVGCRVGAKNTLRKN
                     YLALAENLGVEIRAMRTVTRLGEVPGSGSGGGDPVYRVLHERTGPVGGRDVQVVTARH
                     VVVAAGAWGTQTLLHAMKEVGELPRLSSYLGHRTRTNSEALLGAVSQRVPPEDVDLTK
                     GVAITTSFHVDEDTHVENCRYGKGSNAMGLLATVAVPGGTGRPRWLELARQVGKDPWG
                     FLRLMPMARRWSERMVIGLVMQSRDNSLAVSGKRGLFGRPGLTSRQGHGHPNPTYIPE
                     GHEAMEALAHRLGEATGEYTAAGGSWFEVFDVPMTAHFLGGATIGSSPDRGVVDGYHR
                     VWGHPGISVVDGAAVSANLGVNPSLTITAQAERAMSLWPNRGDADPRPSQDEPYAVVA
                     PVAPVRPAVPAGFPRRPPGPVPVPVSGPVPMSGPVSVGHATMGG"
     gene            complement(1989..3578)
                     /locus_tag="PL014_08505"
     CDS             complement(1989..3578)
                     /locus_tag="PL014_08505"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HOT57169.1"
                     /translation="MADVIADPELDPAATYAVDPARVRALTARVIASDGEGELRTTTP
                     MTGAPLAVLPVSSAGDVVRAVDLARAAQRGWEATPVTERAAVMLRLHDLLLDRQPQLL
                     DLVQLESGKARGHAFEEIGDCAIVARHYARRGPSLLADTHHVGVLPLLSRAVEHHRPK
                     GVVGIVAPWNYPLSLAVSDAIPALIAGNAVVLRPDPQGSLTALAAAELFAEAGLPDGV
                     LQVVLGPGDPTGQAVVDHTDYVSFTGSTATGRMVAQSAARRLVGVSLELGGKNTMYVA
                     ADANLRRAVDGAVRACFSGAGQLCISAERLVVHADVYDEFVPRFVERVSRMRLSTGLH
                     WGADMGSLVSADQKAKVLSHVEDAVSKGARVLTGGRARPDIGPLVVEPTVLEGVTSAM
                     ACRDEETFGPVVSLYRVHSDDEAVRLANDTEYGLNASVWTRDVRRGRAIAARIHAGTV
                     NINEGYAAAWGSVAAPMGGMKSSGLGRRHGREGLLKYTETQNITSQHLVGIAPMAGMT
                     DQGFARSLTGALRIMKALRMP"
     gene            3706..8598
                     /locus_tag="PL014_08510"
     CDS             3706..8598
                     /locus_tag="PL014_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOT57170.1"
                     /translation="MSDNPGRVDIHYVLDKLYYSHDNPRDKGTGFERFIKSFLVTEPL
                     YAGRFEQVWMWSDWPERGSRPDHGIDLVARERDTGDLVAVQCKFFDPSAALPKGEIDS
                     FLAESGKHPFRSRLLVTTAGRLNSAAEAAIEDQQIPVNVIGLEDLVGSTLDWAQFDPS
                     TPEVLATLGRKRLRPHQRTAIDAVRAGLVEHDRGQLIMACGTGKTFTSLRLAEEVVPP
                     GGRVLFLVPSIALLNQSLKEWSTEATDPLRTFAVCSDTKVGKANEDSSVVDLAIPATT
                     DSARLAERASAPPTDPNRRTVVFATYQSLDVVAQAQQAGLPSFDLVICDEAHRTTGVT
                     VAGSDESAFVRVHDSAYLTASKRLYMTATPRLYDEASKVKAGQADAVIASMDDVATFG
                     PEFHRLGFGQAVTAGLLTDYKVLVLTIDEGAVARTFQDDLAMNAELNLPDVARIVGCW
                     NALAKRMAVTADGSGSFATDPEPMCKAVAFAANIKASKRFAETFGQISGTYLDALQAA
                     DSEALEGASSGRAPLQARVRHVDGTMNMLARGELMEWLKATSRDDANEARILSNAKCL
                     SEGVDVPSLDAVMFLNPRKSVVDVVQSVGRVMRLAAGKQYGYIILPIAIPAGMTPEQA
                     LADNERFKVVWEVLQALRAHDERFNAMVNTIELNKARPPQVDIFHITDTPDRDDPPEG
                     PAAPRAVPYQPMLQLEALDNWREAIYARMVDKVGSRRYWETWAKDVADIVERHTTRLR
                     TLITQPETAAQFEEFVAALRANLNDSITAEAAIDMLSQHLVTKPVFDALFPGYEFTDH
                     NPVARVMQAMLTALEGSNLESETEALAEFYESVRLRAAGIDNAEGKQRVITELYEKFF
                     RLAFPRSADALGIVYTPTQIVDFILRSVNDLLVTEFGTSISAPGVHVLDPFTGTGTFI
                     TRLLASGLIAPEDLARKFTTELHANEITLLAYYIAAANIEATFHDLTASFAADAVAPA
                     SADPRDWVPFDGIVLADTFQMSEHEDTLDGLVFRTNSERAERQLDLDIRVIVGNPPYS
                     VGQTSGNDNNANLRYPSLDARIEATYADRSSATNKNSLYDSYIRAIRWASDRVGDAGV
                     VGFVTNGGFVDSNTADGLRKSLIDEFSAVYVYNLRGNQRTAGELSRREGGKVFGAGSR
                     NTVAITFLVRTPGYTGPATLHYRDIGDYLSREDKLAIVQSGHLASIDWQQVTPNAAGD
                     WINQRGEEFDTFQPIGSKPPGAIFAVYSGGVKTNRDAWVYNFSKTTLTDTVDRMVSFY
                     NAEVSRYEAAATPKPPVDGFINTDPTKFSWNRADKVQLTRGRTYQARPEALRLGIYRP
                     FTCQHVWFDPELNDMVYRTPAMFPTAAHENYGIFQVGAGSAVPFSVIAVDALPNLHVT
                     GAGSGGQFFARYTYEATDSGSAQGALNFGSGEGDVIDGYRRLDNITDETLTAYRAWYG
                     EDVTKDGIFASVYGLLHSPDYRARFAADLKRSLPRIPRVEADDFAAFAEAGQRLLDLH
                     IGYEDVQPWPLTVTGDHLPADADPYAWFRVEKLKWGGGARDKDRSRIVYNPRVTITGI
                     PDEAHGYLLGSRTALEWIIDRYQVKTDKPSGIVNDPNDYAREVANPRYILDLIAKVTR
                     VSVETVQIVNTLPRLRIVEG"
     gene            8600..>8967
                     /locus_tag="PL014_08515"
     CDS             8600..>8967
                     /locus_tag="PL014_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57171.1"
                     /translation="MPATEFSEFTYGYAVMRETETFLTKHAGALTRAPEQPSLIKENT
                     VGYDAKFVTVDFAVLLQFKVPFFTSRQHSRGACGPDHCTWSAWRREHFRVWVDVGSNQ
                     QSAMREYEREIDSGAYLGLS"
BASE COUNT         1441 a         3252 c         2997 g         1277 t
ORIGIN      
        1 cctcgacgcg caccgacccg aagccccccg tggcgtcccc gacgagctcg tcgacgtcgt
       61 cgctgacgtc ctcgggcacg gtgacgaagc gccaagcacc ctccttccag cgccagatcg
      121 tcgcggtgaa ggtccagcca ccgggtggag cgctgctcat ccccccattg tcgcgtggcc
      181 caccgacacc ggccccgaca tcggcaccgg ccccgacacc ggaaccggaa ccggccccgg
      241 cgggcggcgc gggaacccgg ccggcacggc ggggcgcacc ggagccaccg gcgccaccac
      301 ggcgtacggc tcgtcctggg acgggcgcgg gtcggcgtca ccacggttgg gccacagcga
      361 catcgcgcgc tcggcctgcg ccgtgatcgt cagcgacggg ttcaccccga ggttggcgga
      421 gaccgcggcc ccgtcgacga ccgagatgcc ggggtgcccc cacacccggt ggtagccgtc
      481 cacgacccct cggtccggag acgacccgat cgtcgccccg ccgaggaagt gcgccgtcat
      541 cggcacgtcg aagacctcga accacgagcc accggctgcg gtgtactccc ccgtggcctc
      601 cccgagccga tgcgcgaggg cctccatggc ctcgtgccct tccgggatat acgtggggtt
      661 cgggtggccg tgcccctgcc gcgaggtcag ccccggccgg ccgaacaggc cccgcttgcc
      721 cgacaccgcc aacgagttgt cccggctctg catgacgagc ccgatgacca ttcgctcgct
      781 ccaccggcgc gccatcggca tcagccgcag gaacccccac gggtccttgc cgacctgtcg
      841 ggcgagctcg agccaccggg ggcgccccgt gccgcccggc acggccaccg tggcgagcag
      901 gcccatcgcg ttcgacccct tgccgtagcg gcagttctcc acgtgggtgt cctcgtcgac
      961 gtggaacgac gtcgtgatcg cgaccccctt cgtgaggtcg acgtcctcgg gcggcacccg
     1021 ctggctcacc gcgccgagca gcgcctcgga gttggtgcgg gtgcggtgcc cgaggtagga
     1081 cgagagtcgt ggcagctcac ccacctcctt catcgcgtgc agcagcgtct gggtgcccca
     1141 ggcgccggcc gcgacgacga cgtgccgcgc cgtcacgacc tggacgtcgc gcccgccgac
     1201 cggcccggtg cgctcgtgca gcacccggta caccgggtca ccgccccccg agccagagcc
     1261 gggcacctct cccagccggg tcacggtgcg catcgcgcgg atctcgacgc cgaggttctc
     1321 ggccagggcc aggtagttct tgcgcaacgt gttcttggcg ccgacccggc atccgaccat
     1381 gcagttgccg cactcggtgc agccggtgcg ccgcggcccg gcgcccccga agtacgggtc
     1441 gtcgacggtc tcaccggggc ggccgaagta caccccgacc ggcgtcttgc ggaacgtgtc
     1501 gccgaccccg aggtcgtcgg cggtgcgtcg catcagctgc tcgacgacgc cctcgcaggg
     1561 gttggtcacc acgccgagca tcgcgctcgc ggtgtcgtag tggggcgcga gctcggcctg
     1621 ccagtcggtg atgtcgctcc actgctcgtc ctcgaagaac ggccgcggtg gcacgtacag
     1681 ggtgttcgcg tagttgagcg accccccgcc gaccccggca ccggccagca cgacgacatc
     1741 ggggagccgg tggattcgct ggatgccaaa gcaccccacc tggggtgccc acaggtagcg
     1801 ccgcacgtcc caggacgtct cggcgaagtc ctcgtcctcg aagcgctggc cggcctccag
     1861 cacgagcacg cggtagccct tctcggccag ccgcagggcg gtgaccgacc ctccgaaccc
     1921 ggagcccacg acgacgacgt cgacgtcgac gtcccgctcg gtagcggcat ccgccgcacg
     1981 gtgtgtcatc acggcatcct caacgccttc atgatccgca gcgcacccgt cagcgagcgg
     2041 gcgaaaccct ggtcggtcat cccggccatg ggggcgatgc cgacgaggtg ctgcgaggtg
     2101 atgttctggg tctcggtgta cttcagcagg ccctcgcgcc cgtggcggcg gcccagcccc
     2161 gacgacttca tgccacccat cggcgcggcg acgctgcccc aggccgcggc atacccctcg
     2221 ttgatgttga cggtccccgc gtgaatgcgc gccgcgatcg ccctgccacg ccgcacgtcg
     2281 cgggtccaga ccgaggcgtt gagcccgtac tcggtgtcgt tggccaggcg gaccgcctcg
     2341 tcgtcggagt gcacgcggta gagcgagacg accggcccga aggtctcctc gtcgcggcac
     2401 gccatggcgg acgtcacccc ctcgaggacg gtcggctcga cgaccagggg gccgatgtcg
     2461 gggcgggcgc ggccaccggt gagcacgcgg gcgcccttgg acacggcatc ctcgacgtgc
     2521 gagagcacct tggccttctg gtcggcgctc acgaggctgc ccatgtcggc gccccagtgc
     2581 agccccgtcg acaggcgcat ccgcgagacc cgctcgacga accgcggcac gaactcgtcg
     2641 tagacgtcgg cgtggacgac gagccgctcg gcactgatgc acagctgtcc ggcgccgctg
     2701 aagcaggccc gcacggcgcc gtcgacggcc cggcgcaggt tggcgtcggc cgcgacgtac
     2761 atcgtgttct tgccaccgag ctcgagggag acgccgacca gccgccgggc ggccgactgg
     2821 gccaccatgc gtcccgtggc ggtcgacccg gtgaagctca cgtagtcggt gtggtcgacg
     2881 acggcttgcc cggtcgggtc ccccggcccg agcacgacct gcagcacccc gtcggggagc
     2941 ccggcctcgg cgaacagctc ggccgcggcc agcgcggtga gcgacccctg cgggtccgga
     3001 cgcagcacga cggcgttccc ggcgatcagc gcggggatcg cgtcggacac ggccaggctg
     3061 agggggtagt tccacggtgc gacgatgccg acgacgccct tgggccggtg gtgctccacg
     3121 gcccgcgaga gcagcggcag caccccgacg tggtgggtgt cggcgagcag cgaggggccg
     3181 cggcgggcgt agtgccgcgc gacgatggcg cagtcgccga tctcctcgaa cgcgtggcca
     3241 cgcgccttgc ccgactccag ctgcacgagg tcgagcagct ggggctgccg gtcgagcagg
     3301 aggtcgtgca ggcgcagcat gacggccgcc cgttcagtga cgggcgtggc ctcccagccg
     3361 cgctgagcgg cccgggcgag gtcgacggcg cgcaccacgt cgccggcgct cgagacgggc
     3421 aggaccgcca gcggtgcccc ggtcatcggg gtggtcgtgc gcagctcgcc ctccccatcg
     3481 gaggcgatga cgcgggcggt gagggctcga acccgggccg ggtccacggc gtacgtggcg
     3541 gcggggtcga gctccgggtc agcgatgacg tcggccatta ccgaagggta accacccggt
     3601 ccgcaccccg gcggcagatg gtgaggagca gcactcgccc gcacccggtc gcgcgaacgc
     3661 cccgctcaac catccaagcc gggctgcgtt ccctagggtg ggcgcgtgag tgacaacccg
     3721 gggcgcgtcg acatccatta cgtgctggac aagttgtact actcgcacga caacccacgg
     3781 gacaagggca ccgggttcga gcgcttcatc aagtcgttcc tcgtcaccga gccgctgtat
     3841 gccgggcgct tcgagcaggt gtggatgtgg tccgactggc ccgagcgcgg cagtcgaccc
     3901 gaccacggca tcgacctcgt cgcccgcgag cgcgacaccg gtgacctcgt ggcggtgcag
     3961 tgcaagttct tcgacccgtc ggcggccctg cccaagggtg agatcgactc cttcctcgcg
     4021 gagtcgggca agcacccgtt ccgctcccgc ctcctcgtga ccaccgcagg gcggctcaac
     4081 tcggctgccg aggccgcgat cgaggaccag cagatcccgg tcaacgtcat cggcctcgaa
     4141 gacctcgtgg gctccacgct cgactgggcc cagttcgacc cgagcacccc cgaggtcttg
     4201 gcgaccctcg gccgcaaacg gctgcgaccc caccagcgca ccgccatcga cgccgtgcgc
     4261 gccgggctgg tcgagcacga ccgcgggcag ctgatcatgg cgtgcggcac gggcaagacc
     4321 ttcacgagcc tgcgcctggc cgaggaggtc gtgccacccg gcgggcgggt gctcttcctc
     4381 gtgccgagca tcgcgctgct caaccagtcg ctcaaggagt ggtcgaccga ggccaccgac
     4441 ccgctgcgca cgttcgccgt gtgctccgac accaaggtcg gcaaggccaa cgaggattcc
     4501 tccgtcgtcg acctggcgat cccggcgacg accgactcgg cccggctggc cgagcgggcc
     4561 agcgcaccac cgaccgaccc gaaccgccgc acggtggtgt tcgcgacgta ccagtcgctc
     4621 gatgtcgtgg cgcaggcgca gcaggctggg ctcccatcat tcgacctcgt catctgcgac
     4681 gaggcccacc gcacgaccgg ggtcaccgtt gcgggcagcg acgagtcggc gttcgttcgg
     4741 gtgcacgaca gcgcctacct cacggcgagc aagcggctct acatgacggc cacgccccgc
     4801 ctctacgacg aggcgtccaa ggtcaaggcc gggcaggcgg atgccgtcat cgcgtcgatg
     4861 gatgacgtgg ccaccttcgg cccggagttc caccgcctgg ggttcggcca ggcggtcacg
     4921 gcgggcctgc tcaccgacta caaggtgctc gtgctgacca tcgacgaggg ggcggtggcg
     4981 cgcaccttcc aggacgacct ggcgatgaat gccgagctga acctgcccga tgtggcccgc
     5041 atcgtgggat gttggaacgc cctggccaag cggatggccg tgacggcgga tggctcaggc
     5101 tcgttcgcca ccgaccccga gccgatgtgc aaggcggtgg cgttcgcggc gaacatcaag
     5161 gcgtcgaagc ggtttgccga gaccttcggg cagatctcgg gaacgtacct cgacgcactg
     5221 caggcggccg actccgaagc ccttgagggc gcctcgtcgg gcagggcgcc cctgcaggcg
     5281 cgcgtgcgcc acgtcgacgg cacgatgaac atgctggccc gcggcgagct gatggagtgg
     5341 ctcaaggcga ccagtcgtga cgatgcgaac gaggcccgca tcctcagcaa cgccaagtgc
     5401 ctgtccgagg gcgtcgacgt cccgagcctg gacgcggtga tgttcctcaa cccacgaaag
     5461 agcgtcgtcg acgtcgtgca gtcggtgggc cgggtgatgc gcctggccgc cgggaagcag
     5521 tacggataca tcatcctgcc catcgcgatc ccggccggga tgacccccga gcaggccctc
     5581 gccgacaacg agcggttcaa ggtcgtgtgg gaggtgctcc aggcgctgcg cgcccacgac
     5641 gagcggttca acgcgatggt caacaccatc gaactcaaca aggcccgccc gccgcaggtc
     5701 gacatcttcc acatcaccga cacgcccgac cgggatgacc caccagaggg cccggccgca
     5761 ccccgcgccg tcccctacca gccgatgctc cagctcgaag ccctggacaa ctggcgtgag
     5821 gcgatctacg cccgaatggt cgacaaggtc gggtcccgcc ggtactggga gacctgggcg
     5881 aaggatgtcg ccgacatcgt cgagcggcac accacccgac tccgcaccct catcacccag
     5941 cccgagaccg ccgcacagtt cgaggagttc gtggcggcgc tgcgggccaa cctcaacgac
     6001 tcgatcaccg ccgaggctgc gatcgacatg ctctcgcaac acctggtgac caagccggtg
     6061 ttcgacgcac tgttcccggg gtatgagttc accgaccaca acccggtggc ccgcgtcatg
     6121 caggccatgc tcaccgcgct cgagggcagc aacctggagt cggagaccga ggcgctggcc
     6181 gagttctacg agtcggtgcg gctacgcgcg gcgggcatcg acaatgcgga gggcaagcag
     6241 cgagtcatca ccgagttgta cgagaagttc ttccggctgg cgtttccgcg ctcggctgac
     6301 gcgctcggca tcgtctacac ccccacgcag atcgtcgact tcatcctgcg cagcgtcaac
     6361 gacctgctcg tcaccgagtt cggcaccagc atcagcgcgc ccggggtgca cgtcctcgac
     6421 ccgttcaccg gcaccggcac cttcatcacc cggctgctcg cgtcggggct cattgccccg
     6481 gaggatctgg cgcgcaagtt caccactgag ctgcacgcga acgagatcac cctgctcgcg
     6541 tactacatcg cggcggcgaa catcgaggcg accttccacg acctcaccgc gtccttcgct
     6601 gcggatgccg tggcgccggc gtccgctgac ccgcgcgact gggtgccctt cgacggcatc
     6661 gtgctcgccg acacgttcca gatgagcgag catgaggaca ccctcgacgg gttggtcttc
     6721 cgcacgaaca gcgagcgggc cgagcgccag ctcgacctcg acatccgggt catcgtcggc
     6781 aacccgccgt actcggtggg gcagacctcc ggcaacgaca acaacgcgaa cctgcgctac
     6841 ccgagcctgg atgcccgcat cgaggcgacg tatgccgacc ggtcgagcgc gacgaacaag
     6901 aacagtctct acgactccta catccgggcg atccgctggg cgagcgaccg ggtcggggat
     6961 gccggggtcg tcgggttcgt gaccaacggc ggcttcgtcg actccaacac cgccgacggg
     7021 ctgcgcaaga gcctcatcga cgagttctcc gccgtgtacg tctacaacct gcgcggcaac
     7081 cagcgcacgg ccggcgagct gtcccgccgg gagggcggca aggtcttcgg cgccgggtcc
     7141 cgcaacacgg tggcgattac cttcctcgtg cgcacgcccg gctacaccgg cccagcgacc
     7201 ctgcactacc gcgacatcgg cgattacctc agccgcgagg acaagctcgc gatcgtccag
     7261 tctggtcacc tcgcgagcat cgactggcag caggtcaccc cgaacgccgc gggtgactgg
     7321 atcaaccagc gcggcgagga gttcgacacc ttccagccga tcggtagcaa gcctccgggc
     7381 gcgatcttcg ccgtgtactc aggcggggtg aagaccaacc gggacgcgtg ggtctacaac
     7441 ttcagcaaga ccacgctgac cgacaccgtc gaccgcatgg tgtcgttcta caacgccgag
     7501 gtcagccgct acgaggccgc ggccacaccg aagccgccgg tcgatggctt catcaacacc
     7561 gacccgacga agttctcgtg gaaccgggcc gacaaggtgc aactcacccg agggcgcacg
     7621 taccaggccc ggcctgaagc acttcgcctc ggcatctacc ggccgttcac ttgccagcac
     7681 gtctggttcg acccggagct caacgacatg gtctaccgga cgccggcgat gttcccgacc
     7741 gcggcgcacg agaactatgg aatcttccag gtcggcgcag gctcggctgt tcccttctca
     7801 gtcatcgcag tggacgccct ccctaacctc catgtgaccg gagccgggag cggcggtcaa
     7861 ttcttcgcga ggtacacgta cgaggccact gactccgggt cggctcaggg tgcgctcaac
     7921 ttcgggagcg gcgagggcga cgtcatcgac ggctaccggc gcctcgacaa catcaccgac
     7981 gagacgctca cggcctaccg ggcctggtat ggcgaggacg tgacgaagga cggcatcttc
     8041 gcctctgtgt acgggctgtt gcactcaccc gactaccggg cgcggttcgc cgcggacctc
     8101 aagcggtcgc tgccgcgcat cccgagggtc gaggccgacg acttcgccgc ctttgccgag
     8161 gctggtcagc gtctgctcga cctgcacatc ggctacgagg acgtccagcc gtggccgctg
     8221 accgtgaccg gcgaccacct gcctgctgat gctgacccgt acgcgtggtt ccgcgtcgag
     8281 aagctgaagt ggggcggtgg tgcccgcgac aaggaccggt cacgcatcgt ctacaacccg
     8341 agagtgacga tcaccggcat ccccgatgaa gcccacggct acctgttggg ctcgcggacg
     8401 gcgctcgagt ggatcatcga ccgctaccag gtcaagaccg acaagccgtc aggcatcgtc
     8461 aacgacccaa acgactacgc ccgcgaggtc gccaacccgc gctacatcct cgacctcatc
     8521 gccaaggtca cccgagtcag cgtggaaacg gtccagatcg tcaacaccct cccacgcctg
     8581 cgaatcgtgg agggctgaga tgccggccac cgagttcagt gagttcacct acggctacgc
     8641 cgtcatgcgg gagaccgaga cgttcctgac gaagcacgcg ggcgccttga ccagggcgcc
     8701 ggagcaaccg tcgctcatca aggagaacac ggtcggctac gacgccaagt tcgtgacggt
     8761 cgacttcgcc gtgctgctgc agttcaaggt gcccttcttc accagccgcc agcactcgcg
     8821 cggcgcgtgt gggccagacc attgcacatg gtcggcatgg cgccgcgagc acttccgggt
     8881 gtgggtcgat gtgggctcga accaacagtc ggccatgcgg gagtacgagc gggagatcga
     8941 cagcggcgcc tacctggggt tgtcgtg
//
LOCUS       DAOSOC010000101         8837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_7893_length_8837_cov_10.332157, whole genome shotgun sequence.
ACCESSION   DAOSOC010000101 DAOSOC010000000
VERSION     DAOSOC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7893_length_8837_cov_10.332157"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PL014_08520"
     CDS             <1..375
                     /locus_tag="PL014_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT57172.1"
                     /translation="GLMHGPTFMGNPLACAIASANLDLLAARDPPGEVTRIERGLSAG
                     LERAASLTSVADVRVLGAVGVVQLREPVRGAEVTAAALERGVWVRPFRDLVYTMPPYV
                     STDDDLATLTAALVGAVGAVHG"
     gene            complement(472..837)
                     /locus_tag="PL014_08525"
     CDS             complement(472..837)
                     /locus_tag="PL014_08525"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HOT57173.1"
                     /translation="MRGRRDVEVGPDAVVPDAGSARRPLSAVLAAFEGGCGSFAEVAT
                     RCDLPLDTVRACVDHLVRMGRLEARELAVGCPSGGCGSCASAAGDASGAGAAAGCGAS
                     GPSAHQSGPVLVSISVPRR"
     gene            complement(834..2864)
                     /gene="feoB"
                     /locus_tag="PL014_08530"
     CDS             complement(834..2864)
                     /gene="feoB"
                     /locus_tag="PL014_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOT57174.1"
                     /translation="MSTSPPAPRSVAAPSCHEDAAAEQAPAGAPVVVLCGAPNVGKST
                     LFNALTGARRTVGNWPGTTVEVGRGACPLGARGAVADVVDLPGSYSLDPMSPDEALTR
                     EVVLAPPPGRPDVVVVVASAAHLARSLYLVAQVRETTLPVVVAVTMGDVAARRGIEVD
                     AVALAAAVGCPVVAIDPRRRSGVDGLVETVSRQLGTADAVPRLASTADPAAGQDHDPA
                     AGPVAAPATDGLDDLAAADERFAWIDTVVTASVRAVGRERRTWSDRIDRFATAPVIGP
                     LLFLAVMWLVFQATTTLAAPLQDALDAFVSGPVSTAGGRLVDAVGLGDSPIRGLVVDG
                     IIAGVGMLLTFVPLMALMFLLLALLEDSGYLARAAVVTDRLMGRLGLPGRAFLPLVVG
                     FGCNVPAISATRILPNARHRVLTALLVPFTSCTARLTVYVLLATTFFPQQAGTVVFAM
                     YLVSILLVVLVGLALRATLWRTVGSDPLVIDLPPYQRPTARLSLAATWLRLEGFLRTA
                     SGIIVATVVVVWLLQSIPVGSGSGAFGEVAVADSLYAELSRAVAPLLAPAGFGAWEAV
                     SALLVGFVAKEAVISSWAQTFALAEPASSREPGALGAQLQQTFDVASGGHTGVAVMAF
                     MVFLLAYTPCVATLAAQRRELGMRWTAFGVVLQLGVAWVLAVAVFQVGRVFW"
     gene            complement(2861..3088)
                     /locus_tag="PL014_08535"
     CDS             complement(2861..3088)
                     /locus_tag="PL014_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOT57175.1"
                     /translation="MSLAQAPIGAQLRVLSTDAPPALTRRLAELGIRPGSHVRCVQRC
                     AGGGRVIDVAGSRIALGRDVLASILLAPVPR"
     gene            complement(3169..3543)
                     /locus_tag="PL014_08540"
     CDS             complement(3169..3543)
                     /locus_tag="PL014_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT57176.1"
                     /translation="MSLSAVTVDVLDPTPPYEQLRRQLADLIGSGVLSPGDRLPPVRQ
                     LAVDLGLAAGTVARTYRELEQAGYVRSRRGGGTRVAPTPPATATPERALEEAAAAYLA
                     RARMLGASAEAATDAVRRAALG"
     gene            complement(3540..4490)
                     /locus_tag="PL014_08545"
     CDS             complement(3540..4490)
                     /locus_tag="PL014_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57177.1"
                     /translation="MRVAIFAVPFALVGLVVALMRRVVVTVALSEHGGVVEIARHTRR
                     WRLVGLLLGGVAAVLLLQGGQQVDALGRVTALAPTALGAGILIGTIIGELTARPKVGI
                     RRSATVETRTLRAVLPRGRAITLAMSSTLLAGALAVGAAWGSADDLGRAGRSFTQTCT
                     VIDPDLGPVLMGSSRGPWPGSFYVVPITVALVVLTGLVALALRAIVTRPRPGLDSHAL
                     DTMLRRWSVGNVLTAATVTVLGTLGPLAVFMASAIFGGSCPNSAVETVVAGVALVIGP
                     VAAGAAFALLASLVLTPSIRVDDLPPSRSGAAAALGAPLP"
     gene            complement(4591..5325)
                     /locus_tag="PL014_08550"
     CDS             complement(4591..5325)
                     /locus_tag="PL014_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57178.1"
                     /translation="MATTGGGGGYPPMATLEGAVPGWPEGRPLTAAVTPEAARAVLQA
                     ESATSVLHPAGLRWGLVRRVGLVVFLMVWLGFAVTMVSGLVSGDRERMPAALMLLGFI
                     ALLVGRALPWGWQRVAPAAGSLPSTRRERLNLNLAAIRGEVTTAPAATWPAGSEAFAL
                     LSATAKSDGIDAPWLFDRAGLPEGVGSQWLAYLDLCGFLTGGGAPLGRWLREPVKITD
                     AGRTHLEAERERLRVLDRLGNHPRVG"
     gene            5452..6579
                     /locus_tag="PL014_08555"
     CDS             5452..6579
                     /locus_tag="PL014_08555"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOT57179.1"
                     /translation="MNSPLDWIRAAAASREAAGLTRRLTTTKAGDGLDLAGNDYLGLR
                     THPAVIAGAVAAAETYGAGAGASRLVSGTLHIHDELESALVALTGAPSALVMSTGYAA
                     NLAALTTLADAGTLIVSDEHTHASLIDGCRLSRAAVTVSRHNDLDHVADLLSRRDAPR
                     AVVVAESIYSVLGDAAPVEDLIALTDEVGALLIIDEAHALGVVGPRGGGLVAAAGQAG
                     AEHVVVTGTLSKAFGSQGGAVLSSPDIREHLVNTARPFIYDTGLAPAAAGAALAALGV
                     LADHPELLDRLGATAARLAEACGVPAPAGAVMSVRMPGPHEAVDAVERARAHGIRIGC
                     FRPPSTPDGSSRLRLTAHADLDDADLERACAVLETLVPDHG"
     gene            6572..7279
                     /gene="bioD"
                     /locus_tag="PL014_08560"
     CDS             6572..7279
                     /gene="bioD"
                     /locus_tag="PL014_08560"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOT57180.1"
                     /translation="MADPRSLTSVVFVTGTGTEVGKTVATAALAAVVHERGSSVAVVK
                     PAQTGLEPGEPGDVDEVRRLAGDDIATHELVRLRDPLSPEAAARRAGVTLPPVAVHAK
                     RIGEIAAESEVVFVEGAGGLLVRLDSRGGTLADLAAGLRYKGIGAGVVLVASAGLGTL
                     NTAALTAEALAARSLPLLGVVIGSWPTEPGLAEESNLIDLPRVTGAPLWGAIPEGAGA
                     LTGEQFRAGVPDWFDLH"
     gene            7431..7943
                     /locus_tag="PL014_08565"
     CDS             7431..7943
                     /locus_tag="PL014_08565"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOT57181.1"
                     /translation="MSKREPYRVCVVCWGNICRSPMGEYLLREAFADAGLADRVVVDS
                     AGTSAEELGNGMHPRTAGVMRRNGHPDTGWSSHRARRFDASWFDRYDLVLPVDHIHVD
                     RLRDLARDDADLAKVRLFRSFDPDAVASGEIGMEDPWYGTDPAYDQTYAEITSAIPGI
                     VEHVRAALGD"
     gene            7948..>8837
                     /locus_tag="PL014_08570"
     CDS             7948..>8837
                     /locus_tag="PL014_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOT57182.1"
                     /translation="MSSLAASTSLADAQPPIALGALDGRYRGTVAALVDHLSEPALNR
                     ARVHVEVEWLIHLTEREVVPGVRRLTASEIESLREMVRTFGAAEIDEMAATERVTQHD
                     VKAVEYFVKKRLTQIAPDDADRGLAELIHFACTSEDVNNLSYAVMVKGAVTEVWLPRA
                     TALVEAVATMASDLRDVPLLAHTHGQPATPTTMGKELAVLAWRLQRQLARIEDAEFLG
                     KLNGATGTYGAHVAAVPGADWQSVSREFVEHLGLTWNPLTTQIESHDWQSELYADIAR
                     FNRVLHNLCTDVWTYISMGY"
BASE COUNT         1372 a         3253 c         3185 g         1027 t
ORIGIN      
        1 gggctcatgc acggcccgac cttcatgggc aacccgctgg cctgcgccat cgcctccgcg
       61 aacctcgacc tgctcgcagc ccgcgacccg cccggggagg tgacccgcat cgaacgtggc
      121 ctgagcgcgg gactggagcg ggcggcatcc ctgacgagcg tcgcggacgt gcgcgtgctg
      181 ggggccgtcg gagtcgtgca gctgcgcgag cccgttcgcg gcgccgaggt gacggccgcc
      241 gccctcgagc gtggcgtgtg ggtgcggccg ttccgcgacc tcgtctacac gatgccgccc
      301 tacgtgagca ccgacgacga cctcgccacc ctcacggccg cgctggtcgg agcggtcggg
      361 gccgtccacg gctgagctac ccgaacgcgg gatccccaga ggctggcgcc gaacacagcg
      421 cgctgcagac agcgcaggat gccgcgccct ctgcagcgct gatggctcga ctcagcgtcg
      481 gggcaccgag atggacacga gcaccggccc ggactggtgc gccgacgggc cgctggcccc
      541 acatccggca gcggcacccg cgccgctcgc gtcccctgca gctgacgcac acgacccgca
      601 gccacccgag gggcagccca ccgccagctc acgagcctcg agccggccca tccgcacgag
      661 gtggtccacg cacgcgcgca cggtgtccag cggtaggtcg caccgcgtcg cgacctccgc
      721 gaacgagcca cacccgcctt cgaaggcggc gagcacggcc gacagcgggc ggcgagcgct
      781 cccggcatcc ggcaccacgg catccggccc gacctcgaca tcccggcgtc ccctcaccag
      841 aacacccggc ccacctggaa gaccgcgacg gccaacaccc acgcgacacc gagctggagg
      901 acgacaccga acgccgtcca gcgcatcccg agctcacggc gctgcgcggc cagggtcgcg
      961 acgcaggggg tgtaggcgag caggaagacc atgaaggcca tgaccgccac ccccgtgtga
     1021 ccgccgctcg cgacgtcgaa ggtctgctgc agctgggcac cgagcgcacc cggctcgcgc
     1081 gaggaggccg gctcggccag ggcgaaggtc tgcgcccacg acgagatgac cgcctccttg
     1141 gcgacgaacc ccacgagcag ggcagacacc gcctcccacg caccgaagcc cgcaggcgcg
     1201 agcagcggcg cgacggctcg ggagagctcg gcgtacaggc tgtcggccac ggcgacctca
     1261 ccgaacgcgc ccgagccgga gccgacgggg atggactgca gcagccagac cacgacgacc
     1321 gtggcgacga tgatgccgga tgccgtgcgc aggaaccctt cgagcctcag ccacgtcgcg
     1381 gcgagactga gccgcgccgt cggccgctga tagggcggca ggtcgatgac gagggggtcg
     1441 ctgccgacgg tgcgccacag ggtcgcgcgc agggccaacc cgacgaggac cacgagcagg
     1501 attgacacga ggtacatcgc gaacacgacg gtgcccgcct gctgcgggaa gaacgtcgtc
     1561 gcgagcagca cgtagaccgt cagccgcgcc gtgcacgacg tgaacggcac gagcagcgcg
     1621 gtgagcaccc gatggcgcgc gttgggcagg atgcgggtcg ctgagatcgc cggcacgttg
     1681 cagccgaagc cgacgaccag cggcaggaac gcccggccgg gcaggcccag gcggcccatg
     1741 aggcggtcgg tgacgacggc cgcgcgggcg aggtagccag agtcctcgag cagcgcgagc
     1801 agcaggaaca tcagcgccat gagcggcacg aaggtcagca acatgccgac cccggcgatg
     1861 atgccgtcga cgacgagccc gcgaatcggg ctgtccccca acccgactgc gtcgacgagc
     1921 cggcctccgg cggtcgagac cggcccggag acgaacgcgt cgagggcgtc ctgcagcgga
     1981 gccgcgagcg tcgtcgtggc ctggaagacc agccacatga ccgcgaggaa gagcagcggg
     2041 ccgatcaccg gcgcggtggc gaaccggtcg atgcggtcgg accaggtccg ccgttcacga
     2101 cccacggcgc gcacggatgc cgtgacgacc gtgtcgatcc acgcgaaccg ttcgtcggcg
     2161 gcggccaggt cgtcgagccc atcggtggcg ggtgcagcga caggcccggc ggcggggtcg
     2221 tggtcttggc cggcggcggg gtcggccgtc gaagcgaggc gcggcacggc atccgccgtc
     2281 ccgagttggc gtgacacagt ctccacgagc ccatcgacac cggaccgacg acgagggtcg
     2341 atcgcgacga cggggcagcc gaccgcggcg gcgagcgcca cggcatccac ctcgatgccg
     2401 cggcgggctg cgacatcgcc catcgtcacg gcaacgacga cgggcagcgt ggtctcgcgc
     2461 acctgggcga cgaggtagag gctgcgggcg aggtgggccg cgctggccac gacgacgacg
     2521 acatcggggc ggcccggggg tggggcgagc acgacctctc gggtcagcgc ctcgtcgggg
     2581 ctcatcgggt cgagggagta ggagccggga aggtcgacga cgtccgcaac ggcgccccgg
     2641 gccccgaggg ggcacgcgcc ccggccgacc tccactgtgg tgccgggcca gttgccgacg
     2701 gtgcggcggg ccccggtcag ggcattgaac agcgtcgact tgccgacgtt aggggcccca
     2761 cagagcacga cgaccggggc accggcggga gcctgctcgg cggcggcgtc ctcgtggcac
     2821 gacggcgccg cgaccgagcg gggtgcgggc gggctcgtgc tcaccgcggc accggcgcga
     2881 gcaggatcga ggcgagcacg tcacggccga gggcgatccg cgagcccgcc acgtcgatga
     2941 cccggccgcc accggcgcac cgttgcacgc accggacgtg gctgccgggg cggatgccga
     3001 gctcggccag gcgccgcgtg agggcgggcg gggcgtcggt ggacagcacg cgcaactgag
     3061 ccccgatcgg ggcctgcgcg agtgacatcg tcaccctcga aacgctagtc ctgatggcgg
     3121 gcgcactggt aatcgggctg ggcgccctga ctggggcgcc gcggccggtc agccgagtgc
     3181 tgcccgacgc acggcatccg tggctgcctc agcggaagcg ccgagcattc gtgcgcgcgc
     3241 gaggtaggcc gcagccgctt cctcgagcgc ccgctcaggg gtcgcggtcg ccggcggggt
     3301 cggtgcgacc cgggtgccgc ctccacggcg cgaccgcacg tagccggcct gctcgagctc
     3361 gcggtaggtg cgagcgacgg tgccggccgc caacccgagg tcgacggcaa gctggcgtac
     3421 gggcggcagg cggtcgcccg gcgagaggac tcctgagccg atgaggtcgg cgagctgccg
     3481 gcgcagctgc tcgtagggcg gcgtcggatc gaggacgtcg accgtgacgg cggacaggct
     3541 catggcagcg gggcgccgag cgcggccgcc gcaccggacc gcgagggcgg caggtcgtcg
     3601 acacggatcg acggggtgag cacgagcgag gcgaggaggg caaaggcagc gcccgcggcg
     3661 acggggccga tgaccagggc aaccccggcc acgacagtct ccacggcact gttgggacag
     3721 ctgccgccga agatcgcgga tgccatgaac accgccaacg ggccaagcgt gccaagcacg
     3781 gtgaccgtgg cagcggtaag cacgttgccg accgaccagc ggcgaagcat cgtgtcgagg
     3841 gcgtggctgt ccaggccggg acgcggcctg gtgacgatgg cgcgcagggc cagggcgacg
     3901 agcccggtga gcacgaccag ggccacggtg atcggcacca cgtagaacga ccccggccac
     3961 gggccacggc tcgagcccat gagcacgggg cccaggtcag ggtcgatgac ggtgcaggtc
     4021 tgggtgaagg agcggcccgc ccggcccagg tcgtcggccg acccccaggc cgccccgacg
     4081 gcgagcgccc ccgcgagcag tgttgacgac atcgcaagcg tgatggcccg acctcgcgga
     4141 aggaccgccc gcagggttcg cgtctcgacc gtggcagagc ggcggatgcc gaccttgggc
     4201 cgggcggtca gctcaccgat gatggtgccg atgaggatgc ccgcacccag ggccgtcgga
     4261 gcgagggcgg tgactcgacc cagggcatcg acctgctgcc cgccttggag caggagaact
     4321 gccgccaccc ctccgaggag cagtccgacc agccgccaac gccgggtgtg gcgggcgatc
     4381 tcgacgacac caccgtgctc ggacagtgcc acggtgacga cgacgcgacg catcagggcg
     4441 acgacgaggc cgacgagagc gaacgggacg gcaaagatgg cgactcgcac gacaaccccc
     4501 ggttgtatcg actgattggt acaagagcag catgaccccg ggggacctct tgtgtcaagc
     4561 acttgtgaca aaccgtcgtc aggatcggcg tcacccgacg cgtgggtgat tcccaagccg
     4621 gtccagcacc cgaaggcgct ctcgttcggc ctcgaggtgg gtccggccgg catcggtgat
     4681 cttcacgggt tcgcgcagcc agcggccgag cggagcgcca ccacctgtga ggaagccgca
     4741 gagatccagg taggccagcc attgggaccc gacgccctcg ggcagtccgg cacggtcgaa
     4801 gagccagggg gcgtcgatgc cgtcgctctt cgcagttgcc gacaggagcg cgaacgcctc
     4861 agaccccgca ggccaggtcg cggcgggggc tgtggtgacc tccccgcgaa tggccgccag
     4921 gttgaggttc aaccgctccc tgcgtgtcga gggaaggctc ccggcagcgg gggccacccg
     4981 ctgccacccc cacggaaggg cgcggcccac aaggagggcg atgaagccca gcagcatgag
     5041 cgctgcaggc atccgctcac gatcgccact gaccaggcca ctgaccatcg tcacggcgaa
     5101 ccccagccag accatgagaa agaccaccag cccgactcga cgaaccaagc cccagcgcag
     5161 acccgcgggg tgcaaaaccg acgtcgcact ctcggcctgc agcacagcgc gagccgcctc
     5221 cggcgtcacc gccgccgtca gcggccggcc ctccggccag ccgggcacgg cgccctccag
     5281 cgtcgccatc ggcgggtacc ccccaccacc cccggtcgtc gccatgaccg cattctccac
     5341 ccaacctcgc gatcgcgcac ggcatccaga gccggatgcc gttgcgccgg ggccgcacca
     5401 actcgcacct tcgaggggcc acgcccagat cggcgtgcaa ggatcgggca ggtgaacagc
     5461 cccctcgact ggatccgcgc cgcggccgcg tcacgcgaag ccgctggcct gacccggcgc
     5521 ctcacgacga cgaaggccgg cgacggcctc gacctcgccg gcaacgacta cctcgggctg
     5581 cgcacccacc ccgccgtcat cgcgggggcg gtcgccgcgg ccgagaccta cggcgcgggc
     5641 gcgggcgcct cccgcctcgt gagcggaacc ctgcacatcc acgacgagct cgagtcggcc
     5701 ctggtggccc tcaccggcgc ccccagcgcc ctcgtgatgt cgaccgggta cgccgcgaac
     5761 ctcgcggccc tgaccaccct cgccgatgcc ggcacgctca tcgtcagcga cgagcacacc
     5821 cacgcgagcc tcatcgacgg ctgccgcctg tcgcgcgccg cagtcacggt ctcgcgccac
     5881 aacgacctcg accacgtcgc cgacctgctc tcgcgccggg acgccccgcg cgccgtcgtc
     5941 gtcgccgagt cgatctactc ggtgctcggc gacgccgccc ccgtcgagga cctcatcgcc
     6001 ctcacggacg aggtcggggc cctcctgatc atcgacgagg cgcacgcgct gggggtcgtc
     6061 gggccccgcg gtggcgggct cgtcgcggcg gccggccagg ccggggccga gcacgtcgtc
     6121 gtcaccggga ccctgtcgaa ggccttcgga tcccagggcg gagctgtgct gtcctccccg
     6181 gacatccgcg agcacctggt caacacggcg cgaccgttca tctacgacac cggcctcgcc
     6241 ccggcagcgg ccggcgcagc actggccgcc ctgggcgtgc tcgccgacca ccccgagctc
     6301 ctcgaccgcc tcggcgccac cgccgcccgc ctcgccgagg catgtggggt cccggcgccg
     6361 gcaggggccg tcatgtcggt gcggatgccg ggccctcacg aagcggtgga tgccgtcgag
     6421 cgggcgcgcg cgcacggcat ccggatcggg tgtttccggc ccccgtcgac ccccgacggc
     6481 tcctcacggc tgcggctcac cgcgcatgcc gacctcgatg acgcggacct cgagcgggcc
     6541 tgcgccgtgc tcgagaccct ggtgcccgac catggctgac ccgcgctcgc tgacctcggt
     6601 ggtcttcgtc accggcaccg gaaccgaggt cggcaagacc gtcgcgacgg ccgcgctggc
     6661 cgccgtcgta cacgagcgcg gcagcagcgt cgcggtggtc aagcctgcgc agaccggtct
     6721 ggagccgggt gagcccgggg acgtcgacga ggtgcgccgc ctcgccggcg acgacatcgc
     6781 gacgcacgag ctcgtgcggc tgcgcgaccc gctctcacca gaggccgccg cgcgccgggc
     6841 cggggtgacc ctgcccccgg tggccgtcca cgccaagcgt atcggcgaga tcgcggccga
     6901 gtcggaggtc gtgttcgtcg agggtgccgg gggcctgctc gtgcggctcg acagccgcgg
     6961 cggcaccctg gccgacctcg cagcagggct gcgctacaag ggaatcggcg ccggggtggt
     7021 gctcgtggcc agtgccgggc tcggcacgct caacacggcc gcgctgaccg ccgaggcgct
     7081 ggcggcgcgc tcgctgcccc tgctgggggt cgtcatcggc tcgtggccga ccgagcccgg
     7141 cctggccgag gagagcaacc tgatcgacct tccgcgcgtc accggggcgc cgctgtgggg
     7201 ggcgatcccg gaaggtgccg gggccctcac cggtgagcag ttccgggccg gcgtgcccga
     7261 ctggttcgac ctccactgac cagcccccga ccccgcccga gccccagccc cagcccctcc
     7321 gttcccgact tcgtgcgact tcggtgcgga ttttcccggt ttgagcgccc cggtgcgcac
     7381 gcaagtcgca cgaagtcggg atgaggtgtc ggggttgaga ggatgggtgg atgagcaagc
     7441 gagagccgta ccgcgtgtgt gtcgtgtgct ggggcaacat ctgccgctcc cccatggggg
     7501 agtacctgct tcgtgaggcg ttcgcggatg ccgggctggc cgaccgcgtc gtcgtcgact
     7561 cggcgggcac ctcggccgag gagctcggca acgggatgca cccgcgcacc gccggcgtca
     7621 tgcgccgcaa cggccacccc gacaccggct ggagcagcca ccgggcccgc cggttcgacg
     7681 cctcgtggtt cgaccgctac gacctcgtgc tgcccgtcga ccacatccac gtcgaccgcc
     7741 ttcgtgacct ggcgcgtgat gacgccgacc tcgccaaggt tcgcctgttc cgctccttcg
     7801 acccggatgc cgtcgcgtcg ggcgagatcg gtatggagga cccctggtac ggcaccgacc
     7861 cggcctacga ccagacctat gccgagatca cgtccgccat cccgggcatc gtcgagcacg
     7921 tgcgcgccgc tctgggagac tgagcgcatg agcagcctcg ccgcctcgac ctcgctcgcc
     7981 gacgcccagc ccccgatcgc gctcggtgcg ctcgacgggc gctaccgcgg gacggtcgca
     8041 gccctcgtcg accacctcag tgagccggcc ctgaaccggg cccgcgtgca cgtggaggtc
     8101 gagtggttga tccacctcac cgagcgtgag gtggttccgg gcgtgcggcg actcacggca
     8161 tccgagatcg agagcctgcg cgagatggtg cgcacgttcg gcgcggccga gatcgacgag
     8221 atggccgcca ccgagcgggt cacccagcac gacgtgaagg ccgtcgagta cttcgtgaag
     8281 aagcggctca cccagatcgc gcccgatgac gccgaccgcg gcctggccga gctcatccac
     8341 tttgcgtgca cgagcgagga cgtcaacaac ctgtcgtacg ccgtgatggt caagggggcc
     8401 gtgaccgagg tctggctgcc gcgggccacc gccctcgtcg aggcggtcgc gacgatggcc
     8461 tccgacctgc gagacgtgcc gctgctcgcc cacacccacg ggcagcccgc gaccccgacg
     8521 acgatgggca aggagctcgc cgtgctggcc tggcggctcc agcgccagct cgcgcgcatc
     8581 gaggacgccg agttcctcgg caagctcaac ggtgcgaccg gcacctacgg ggcgcacgtc
     8641 gccgcggtgc ccggtgccga ctggcagtcg gtgagccgcg agttcgtcga gcacctcggc
     8701 ctgacgtgga acccgctcac gacgcagatc gagagccacg actggcagag cgagctctac
     8761 gccgacatcg cccggttcaa ccgcgtgctg cacaacctgt gcaccgacgt gtggacctac
     8821 atctcgatgg ggtactt
//
LOCUS       DAOSOC010000102         8725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_8037_length_8725_cov_8.747174, whole genome shotgun sequence.
ACCESSION   DAOSOC010000102 DAOSOC010000000
VERSION     DAOSOC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8037_length_8725_cov_8.747174"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PL014_08575"
     CDS             complement(<1..578)
                     /locus_tag="PL014_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HOT57183.1"
                     /translation="MNPHARKVLQGVVGIGLAVALLWWGLPYFAKTTWHDIGTVISSV
                     PVSHAIGFQVLMLAGLWCYTFTFTGSLPGLTHSKALVVNLCGSSVSNLLPGGGAVGLA
                     ATYAICRSWGFSRRATSTSVVVTGVWNVLARIALPVIAIAVLWFSGVTLPGALTDLAV
                     AGMVTGLAILTTLVSIIASERAAQAIGRGLDAV"
     gene            complement(575..>1117)
                     /locus_tag="PL014_08580"
     CDS             complement(575..>1117)
                     /locus_tag="PL014_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT57184.1"
                     /translation="APLEEAAERLAAILRTERADLLISYDANGGYGHRDHVRVHEVGA
                     RAAVIAGTPRVLEATVPRDTIARAIRAVARVYRFPAEFDPTSFERAFTARADITHRID
                     VRKHIAAKRSSMRAHASQATGGGAAAVGEGGQTGDRTLAAFLRIPRPLYDVVFGREWF
                     RDPALPSGGPIHTQIFEGDA"
     assembly_gap    1118..1217
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1343..2467
                     /locus_tag="PL014_08585"
     CDS             1343..2467
                     /locus_tag="PL014_08585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT57185.1"
                     /translation="MLSDCYPPRLGGIESQVRDLSRHLVAAGHEVEVFTATPGPGGER
                     GGATERGDDGVTVHRHAWGVPGGIPVNPFAPPEIRRRLDAGGFDVAHAHLGVVSPFAT
                     DLVPVALEVGLPVAATFHCVMERSAVVCRAIGHLDRWARRGVALSAVSTMAAQRVSAA
                     ARGARVEVVPNGVDAAWWHPGGAATASAHHDGSVHVVSAMRLVSRKRPLAMLRVLEQA
                     RAVLDPAVALRATIVGEGPQRRVMEHRLRARGLTWVQMPGRVDKDELRALHQGADVYL
                     SAAQLEAFGIAALEAQAAGLPIVARRGTGTDDFVKDGVSGLLADSDAALAGALAHLAG
                     ERALREQMRAHLLASRPAQDWPGVITATLGEYRRAAVALR"
     gene            2464..3132
                     /locus_tag="PL014_08590"
     CDS             2464..3132
                     /locus_tag="PL014_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT57186.1"
                     /translation="MITVVGEHAGREVVSFALAHGEDPVAGLRARGWQAQVEAVEGVL
                     GDLTFRYAVVPTEDGSANDPAGPEGPGLSRAERVHIVPHQRVAAYAVVVAQGRLLLTQ
                     LAPRTGAAGRWNLPGGGVDPGESPVDAVVREVAEETGQVVDDVRLIDVMTQHWVGRSA
                     RGLEDYHAVRVLHTARCATPTHPVVHDVGGSTSDARWVALDDLTSVELVASVPVALRA
                     AGLA"
     gene            complement(3164..3478)
                     /locus_tag="PL014_08595"
     CDS             complement(3164..3478)
                     /locus_tag="PL014_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57187.1"
                     /translation="MSIPTTPSTPATPGTPDDSLATSAPHSPATQAPALPEPVYLEGP
                     APFAVVLGLLGLLFASTTLFTQLTDVAVPWTQLGPWTVVAAGLLVVVIGAIGLRSSRT
                     RG"
     gene            complement(3478..4845)
                     /locus_tag="PL014_08600"
     CDS             complement(3478..4845)
                     /locus_tag="PL014_08600"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOT57188.1"
                     /translation="MTQNPPSTEPGAGAPPPPSSSSFLDDSFARLRGLGPVRDTERRW
                     FGGVCSGLAGRLGVDPILIRAAAIILAFVGGIGITAYVLLWLLLPDLRGEILAERAIR
                     HGDVGAIVLLVVAGLLVFGGIASAGSGDGWIVPLWLIPVALVAWFVLNRRGTFGGSPP
                     PPMPMPPGGTMSAPAPRSATAPTSATASTSPTDTTAPYGGYTPSAPQQYAAPYGGYQA
                     PYGGYPAPPAPPRPVAPPPPPRPRRRRPSAFIGLVSLGIAIAGIGLGVALHDPTGFPG
                     SASTLGFLIALTGVSLVVLALGLRGRASGFSGFLAFCLAGLLAISLIDARVQTSNGVG
                     ERTWSPVPATGVTTFELGAGDAVLDLGSMITPDPRITPQTIEVNMGAGELTILVPDGL
                     DARVDASVGFGEIRHIGGTGTSTDTSGNSGGQDQKKTVRVGDTPVQVIVDAELGLGQI
                     TIQEQ"
     gene            4972..6240
                     /locus_tag="PL014_08605"
     CDS             4972..6240
                     /locus_tag="PL014_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOT57189.1"
                     /translation="MPTPYEPAAAHPRAGMPPRPEVSGPPRPPLVRARGRGWTPGVCA
                     AIARHLGLSPKAVRWSFVILTAFGGAGVVAYAFLWALTPEGDVVDDGTGQVAVASDRR
                     QWGSLILIGAALVLVGGVLVLPILGGSLIGSVLLPLFTIAVGAIVAWSNLDEAQRSRW
                     LESGTGPNRFGWMRVALGVGLTVTGILVLVTRGQSFSAVWDALLAAIAVLVGALIIAA
                     PWAMRLWGDLRREQVAAARATERADIAAHLHDSVLQTLALIQRQSGDAAAVTRLARAQ
                     ERELRTWLYDGPQGSQSTLAAAVTEVAHEVEDLHGQVVDLVVTGDRPYEPHGAALTQA
                     LREALLNAVRHGRAPVTAYVEIGPSGVEAFVRDRGTGFDLDDVPADRLGVRQSILGRM
                     ERHGGSARVRRRDDGTEVELRLPPLQGDQP"
     gene            6237..6965
                     /locus_tag="PL014_08610"
     CDS             6237..6965
                     /locus_tag="PL014_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT57190.1"
                     /translation="MTTTRAPSGTSPRPAVRVVLVDDHRMFRAGVRAELTEAMAAAGG
                     AAACEFVGEAPDVDTAIAVIRAERPDVVLLDVHLPGGSGNGGADVIRGSYGVETDAGA
                     PVRFLALSVSDAAEDVIAVIRAGARGYVTKTIGARDLITAIRRVADGDAVFSPRLAGF
                     VLDAFGAAAGEIAEVDEELDRLSAREREVMRLIARGYQYKEVAKELFISVKTVETHVS
                     SVLRKLQLSSRHELTAWAMGRRLL"
     gene            6991..7332
                     /locus_tag="PL014_08615"
     CDS             6991..7332
                     /locus_tag="PL014_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57191.1"
                     /translation="MEIVEHLLVLVHLLGMAAIVGSAVFVARGTVTPALVWGARAQLV
                     TGLILVGLAEMDDGDVNHAKIGVKLVVAIAVAACAEIAAAKGKKGEARPQLVTAAGAL
                     ALLNTAIAVLW"
     gene            complement(7378..7734)
                     /locus_tag="PL014_08620"
     CDS             complement(7378..7734)
                     /locus_tag="PL014_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOT57192.1"
                     /translation="MSDVTILHNRNCSTSRFATEAAASAGVAADVVEYLKQPLDAAAL
                     ADLLDKLEDPPTDLVRRDAFFTQSGLTDADVQTREQIIAVLVEHPRLLQRPVLVKGDR
                     AIIGRPKDRVADFLES"
     gene            complement(7757..>8725)
                     /locus_tag="PL014_08625"
     CDS             complement(7757..>8725)
                     /locus_tag="PL014_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HOT57193.1"
                     /translation="RALVVTRPGGPEVLEVQDREIPTPGPGEALIAVAAAGINFIDVY
                     QREGIYPTPTPFVLGREGAGVVLAVGPDVEGVAAGDRVAWPFADGSAASHACIPADGL
                     VTVPEGVDLETAAASILQGLTAHFLTRTTYPVGPGTTALVHAAAGGVGQLLVQMIKAL
                     GGTVIATAGSDEKCSTAAGLGADHTINYRTVTDLAAAVRDATGGTGVDVAYDGVGKDT
                     FDASLASLKPRGLLALYGGASGQVPPFDLQRLNAAGSVFVTRPSLPHHIATPDELAWR
                     AGELFQSIEDGSLRVSVGGRYPLAEASTAYADLEARRSQGKLIFIP"
BASE COUNT         1136 a         2942 c         3316 g         1231 t
ORIGIN      
        1 acggcatcca acccccggcc gatggcctgg gcggcccgct cgctcgcgat gatcgacacc
       61 aaggtggtga ggatcgccag gccggtgacc atccccgcca cggccaggtc ggtcagggcc
      121 ccgggcaggg tgacgccgct gaaccagagc acggcgatcg cgatcacggg gagggcgatg
      181 cgggccagga cgttccacac cccggtgacg acgaccgagg tcgaggtcgc gcggcgggag
      241 aacccccagg aacgacagat cgcgtacgtg gcggcgaggc cgacggcgcc accaccgggg
      301 aggaggttgg agaccgatga cccgcacagg ttgacgacca gcgccttgct gtgggtcagc
      361 cccggcagcg aaccggtgaa ggtgaaggtg tagcaccaca gcccggcgag catgagcacc
      421 tggaagccga tggcgtggct gacggggacc gaggagatga ccgtgccgat gtcgtgccac
      481 gtcgtcttgg cgaagtacgg cagaccccac cacaacaggg ccacggccag gccgatcccg
      541 accacgccct ggagcacctt gcgtgcgtgc gggttcacgc gtctccctcg aagatctggg
      601 tgtggatggg ccccccgctg ggcagggccg ggtcgcggaa ccactcccgg ccgaagacga
      661 catcgtagag aggccgcgga atgcgcagga acgcggcgag ggtccggtcg ccggtctggc
      721 caccctcgcc gaccgcggcc gcaccaccac cggtcgcctg cgaggcgtgg gcgcgcatcg
      781 acgaccgctt ggccgcgatg tgcttgcgca cgtcgatgcg gtgggtgatg tcggcgcggg
      841 cggtgaaggc ccgctcgaac gacgtcgggt cgaactcggc cgggaaccgg tagacccgag
      901 cgaccgcgcg gatggcccgg gcgatcgtgt cgcgcgggac cgtggcctcg agcacgcggg
      961 gggtgccggc gatcacggca gcccgtgcgc ccacctcgtg cacccgcacg tggtcgcgat
     1021 ggccgtagcc gccgttggcg tcgtaggaga tgagcaggtc ggcgcgttcg gtgcgcagga
     1081 tcgccgcgag tcgctcggcg gcctcctcga gcggggcnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnagg cgcgccatcg tgccggccgt cagcagggcc tcgtcgtcgg
     1261 ggtgggcatg gaacgccacg atcgttgcgg acatcgccgc ccatcctcgc gcatggcagg
     1321 ctgtccgctc gtgaaggtcg ccatgctctc tgactgctac ccgccgcgcc tcggcgggat
     1381 cgagagccag gtccgcgacc tctcccgcca cctcgtggcc gccggacacg aggtcgaggt
     1441 cttcacggcc acccccggac ctggtggcga gcgcggcggc gcgaccgagc gcggtgacga
     1501 cggcgtgacc gtgcaccggc acgcgtgggg tgtgccgggc ggcatccccg tcaacccgtt
     1561 cgccccgccg gagatccgcc gacggctgga tgccgggggt ttcgacgtcg cgcacgccca
     1621 cctgggcgtc gtgagcccct tcgcgaccga tctcgtgccg gtcgcgctgg aggtcggtct
     1681 tccggtcgcg gccacgttcc actgcgtcat ggagcggtcc gcggtggtgt gccgggcgat
     1741 cgggcacctt gatcgctggg cccggcgtgg cgtggcgctc agtgccgtgt cgacgatggc
     1801 tgcccagcgg gtgtccgccg ctgcccgcgg agcccgcgtc gaggtggtgc ccaacggcgt
     1861 ggatgccgcg tggtggcacc cgggtggtgc cgccacggca tccgcgcacc acgacggctc
     1921 cgtgcacgtc gtctccgcga tgcggttggt gtcgcgaaag cgaccgctcg cgatgctgcg
     1981 ggtgctcgag caggcgcgcg ccgtgctgga tcctgcggtc gcgctgcgcg cgacgatcgt
     2041 cggtgagggg ccgcagcgtc gggtcatgga gcaccggctg cgcgctcgcg ggctcacctg
     2101 ggtccagatg cccggtcggg tggacaagga cgagctgagg gccctgcacc agggcgcgga
     2161 cgtctacctc agtgcggccc agctcgaggc gttcggcatc gctgcgctcg aggcccaggc
     2221 ggcggggttg ccgatcgtcg cccggcgcgg caccggcacc gacgacttcg tgaaggacgg
     2281 ggtgagcggg ttgctggccg acagcgacgc cgccctggcc ggtgcactgg cccacctggc
     2341 cggtgagcgg gcgttgcgcg agcagatgcg cgctcacctg ctggcctctc gtccggccca
     2401 ggactggccc ggggtcatca cggccacgct cggtgagtac cgcagggccg cggtggccct
     2461 gcgatgatca ccgtcgtcgg ggagcacgcg gggcgcgagg tggtgtcgtt cgccctggcc
     2521 cacggggagg acccggtcgc gggcctgcgt gcgcggggct ggcaagcgca ggtggaggcg
     2581 gtcgaggggg ttcttggcga cctgacgttc cggtacgccg tggttcccac ggaggacggg
     2641 tcggcgaacg acccggcggg gcccgagggc ccggggctgt cgcgggccga gcgggtgcac
     2701 atcgtgccgc accagcgggt ggcggcgtac gcggtcgtcg tggcgcaggg ccgcctgctg
     2761 ctcacgcaac tggctccacg gaccggtgct gccgggcggt ggaacctgcc cggcggcggc
     2821 gtcgaccccg gcgagtcccc ggtcgacgcg gtcgtgcgcg aggtggccga ggagaccggc
     2881 caggtcgttg acgacgtccg gctgatcgac gtcatgaccc agcactgggt gggccggtcc
     2941 gcgcgggggt tggaggacta ccacgcggtg cgggtgctcc acacggcccg gtgtgccacg
     3001 ccgacccacc ccgtcgtgca tgacgtgggt gggtcgacga gtgacgcgcg ctgggtcgcc
     3061 ctggacgacc tgacgtcggt cgagctcgtg gcgagcgtgc cggtcgcgct ccgtgccgcc
     3121 gggctcgcct gaggccacgg ctcagtcacg cttctgccgc ggctcagccg cgggtacggc
     3181 tgctgcgcag tccgatcgcg ccgatcacga cgacgagcag gcccgccgcg acgacggtcc
     3241 acgggccgag ctgggtccac ggcacggcga cgtccgtcag ctgcgtgaac agcgtcgttg
     3301 acgcgaacag cagcccgagc agcccgagca ccaccgcgaa cggggcaggc ccctcgaggt
     3361 acacgggttc ggggagcgcg ggtgcctggg tggcgggtga gtggggggcg ctggtggcaa
     3421 ggctgtcgtc gggcgtgccc ggggtggcgg gtgtgctcgg ggtggtgggg atgctcatca
     3481 ctgctcctgg atggtgatct ggccgagtcc gagctcggca tcgacgatga cctggacggg
     3541 ggtgtcgccg acccgcacgg tcttcttctg gtcctggccg ccggagttcc cggaggtgtc
     3601 ggtgctggtt cccgtccctc cgatgtgcct gatctcgccg aacccgacgc tggcgtcgac
     3661 ccgggcgtcg agcccgtccg gcacgaggat ggtcagttca cctgcgccca tgttgacctc
     3721 gatggtctgg ggggtgatgc gggggtcggg agtgatcatc gagccgaggt cgaggacggc
     3781 gtccccggcc cccagctcga acgtcgtcac gccggttgcg ggaaccgggc tccacgtgcg
     3841 ctcgccgacc ccgttgctcg tctgcaccct cgcgtcgatg agcgagatgg cgagcagtcc
     3901 ggccaggcag aaggcgagga agccgctgaa gccggaggcg cgaccgcgca ggccgagcgc
     3961 gagcacgacg aggctcacgc cggtgagagc gatgagaaag cccagggtcg atgcactccc
     4021 ggggaaacct gtcgggtcgt ggagggcgac ccccaggccg atgcccgcga tggcgatgcc
     4081 gagggacacc aacccgatga aggcgctggg ccggcgtcgg cgcggtcgcg gcggcggggg
     4141 cggggccacc ggccgcgggg gcgccggcgg tgcggggtag ccaccgtagg gagcctggta
     4201 gccgccgtag ggtgccgcgt actgctgggg agccgacggg gtgtacccgc cgtagggggc
     4261 ggtcgtgtcc gtcggagagg tggaggccgt ggcggacgtg ggggccgtgg cggacctggg
     4321 ggcaggtgcg gacatggttc ctcctggggg catgggcatg gggggtggtg gcgagccgcc
     4381 gaaggtgccg cgccggttca gcacgaacca cgcgaccagc gcgacgggga tcagccacag
     4441 ggggacgatc cacccgtccc cggacccggc cgacgcgatg ccgccgaaga cgaggaggcc
     4501 ggcgacgacg agcagcacga tggccccgac gtcgccgtgc ctgatggcgc gctcggcgag
     4561 gatctcgccg cgcaggtcgg gcaggaggag ccacagcagg acgtacgcgg tgattccgat
     4621 gcctccgacg aaggccagga tgatcgccgc cgccctgatg aggatcgggt cgacgcccag
     4681 ccggccggcc agccccgagc acacgccgcc gaaccagcgg cgctcggtgt cacgaacggg
     4741 gcccagacct cgcagccggg cgaaggagtc gtcgaggaat gaggagctcg atgggggcgg
     4801 gggagcgcct gcgccgggtt cggtcgaggg cgggttctgc gtcatggccc cagcctcgcc
     4861 cgccagcagc tgctgccgcc atgagggtcg accctgaggc ccccctgacc gcacccctga
     4921 gcgacccctg tcgggggccc cgtgcggctc ccggtacgtc aggatggggg cgtgcccaca
     4981 ccgtacgaac ccgcagccgc gcatccgcgc gccgggatgc cgccgcgccc ggaggtgagc
     5041 ggcccgcctc ggccgccgct ggtcagggcc cggggccggg gctggacccc cggagtgtgt
     5101 gcggcgatcg cccgccacct cggcctgtca cccaaggcgg tgcggtggag cttcgtcatc
     5161 ctcactgcct tcggtggcgc aggagtcgtc gcgtatgcct tcctgtgggc gctcaccccg
     5221 gagggtgatg tcgtggatga cggaaccggc caggtcgcgg tcgcctccga tcggcgccag
     5281 tggggttcgc tcatcctcat cggcgcggcg ctggtgctcg tcggcggcgt gctcgtcctg
     5341 ccgatcctcg ggggctcgct catcgggtcg gtcctgctgc cgctgttcac catcgccgtc
     5401 ggggcgatcg tcgcgtggtc caacctcgac gaggcccagc gttcgcgttg gctggagtcc
     5461 ggtaccgggc cgaaccgctt cggctggatg cgcgtggccc tcggcgtggg tctcaccgtc
     5521 accggcatcc tcgtgctcgt gactcgtggc cagtccttct cggccgtgtg ggacgcgctg
     5581 ctcgcggcga tcgccgtgct cgtcggcgcc ctgatcatcg cggcaccgtg ggctatgcgc
     5641 ctgtggggtg atctgcggcg ggaacaggtg gctgcggcgc gggcaaccga gcgggctgac
     5701 atcgcggcac acctgcacga ctcggtgctc cagaccctgg ccctgatcca gcgccagtca
     5761 ggcgacgccg cggccgtcac ccgtctcgcc cgggcccagg agcgcgagct tcggacctgg
     5821 ctctacgacg ggccccaggg gtcacagtcg accctggctg ccgcggtcac cgaggtcgcc
     5881 cacgaggtcg aggacctcca cgggcaggtc gtcgacctcg tcgtcaccgg ggaccggccc
     5941 tacgaacccc acggggcggc gctcacccag gcgctgcgcg aggccctgct caacgcggtg
     6001 cgccacggtc gtgcccccgt gacggcctac gtcgagatcg ggccctccgg ggtcgaggcc
     6061 ttcgtgcgcg accgcgggac cggcttcgac ctcgacgacg ttcccgccga ccggctcggc
     6121 gtgcgacagt cgatcctcgg gcggatggaa cggcacggtg gcagcgcccg cgtgcgccgc
     6181 cgcgacgacg gaaccgaggt ggagctgcgg ctgccacccc ttcaaggaga tcagccatga
     6241 ccacgacccg agcaccctcc ggcacctctc cccgtcccgc ggtgagggtg gtgctcgtcg
     6301 acgaccaccg gatgttccgc gccggggttc gtgccgagct caccgaggcg atggccgctg
     6361 ccggaggggc agccgcgtgt gagttcgtcg gggaggcacc cgatgtcgac accgcgatcg
     6421 cggtcatccg ggccgagcgt ccggacgtcg tgctcctcga cgtgcacctg cccggtggct
     6481 cgggcaacgg cggcgccgac gtcatccgcg gctcctacgg ggtcgagacc gacgcggggg
     6541 cgccggtgcg cttcctcgcg ctctcggtct cggatgccgc ggaggacgtc atcgccgtca
     6601 tccgcgccgg ggcgcggggg tacgtgacca agaccatcgg ggcccgcgac ctcatcaccg
     6661 ccatccggcg ggtcgcggac ggggatgccg tgttctcgcc ccggctggcc ggcttcgtcc
     6721 tcgacgcctt cggggcggcc gccggtgaga tcgccgaggt cgacgaggag ctcgaccggc
     6781 tctcggcacg cgaacgtgag gtcatgcgcc tcatcgcccg cggctaccag tacaaggagg
     6841 tcgccaagga gctgttcatc tccgtgaaga ccgtcgagac ccacgtgtcg tcggtgctgc
     6901 gcaagctcca gctgtcctcg cgccacgagc tgacggcgtg ggccatgggg cggcgcctgc
     6961 tctgagttcc gggtctccta ggctgctccc atggagatcg tcgaacacct gctcgtcctc
     7021 gtccacctgc tcggcatggc cgcgatcgtc ggcagcgccg tgttcgtagc ccgcgggacg
     7081 gtgacgcccg cactggtctg gggtgcacgg gctcagctgg tcacgggcct gatcctcgtc
     7141 ggtctggccg aaatggatga cggcgacgtc aaccacgcca agatcggggt caagctggtc
     7201 gtcgcgatcg cggtcgcggc ctgcgccgag atcgcggccg cgaaggggaa gaagggtgag
     7261 gcgcgtccgc aactggtgac ggcagcgggt gccttggcgc tgctcaacac cgcgatcgcc
     7321 gtcctctggt gagtcgcggg cgatgactcc gtggccggtc taggagttga ggaccggtca
     7381 ggactcgagg aagtcggcca cgcggtcctt gggtcggccg atgatcgccc gatcgccctt
     7441 gaccagcacg ggccgctgaa gcagccgtgg gtgctccacg agcaccgcga tgatctgctc
     7501 gcgggtctgc acgtcggcgt cggtgagccc gctctgggtg aagaacgcgt cgcggcgcac
     7561 gagatcggtc ggggggtcct cgagcttgtc gagcaggtcg gccagtgcgg cggcgtccag
     7621 aggctgcttg aggtactcga cgacgtcggc ggccacgccg gctgaggccg cggcctccgt
     7681 cgcgaaccgc gaggtcgagc agttccggtt gtgcaggatc gtcacgtcac tcatggcgac
     7741 acggtagccc tctcggtcac gggatgaaga tcagcttgcc ctggctgcgt cgtgcctcga
     7801 ggtctgcgta ggcggtgctg gcctcagcca gcgggtagcg gcctccgacg ctcacgcgca
     7861 ggctgccgtc ctcaatggac tggaagagct cgcccgcgcg ccaggccaac tcatcgggcg
     7921 tggcgatgtg gtggggcagg ctgggcctcg tcacgaagac cgaccccgcg gcattgagtc
     7981 gttgcaggtc gaacggcggc acttggccgg aggcaccgcc gtagagcgcg agcagacccc
     8041 gcggcttcag cgacgcgagc gaggcgtcga aggtgtcctt gccgactccg tcgtacgcga
     8101 cgtcgacccc ggtgccgccg gtggcgtcgc ggaccgcggc ggccaggtcg gtgacggtgc
     8161 ggtagttgat cgtgtggtcg gcgcccagcc cggcggcggt ggagcacttc tcgtcactgc
     8221 cggcggtggc gatgacggtt ccgccgagag ccttgatcat ctggacgagc agttggccca
     8281 ccccgccggc agccgcgtgc acgagtgcgg tcgtgcccgg gcccaccggg taggtggtgc
     8341 gggtcaggaa gtgtgccgtg agtccttgga ggatcgaggc tgccgcggtc tcgaggtcga
     8401 caccttctgg gacggtcacg agcccgtctg ccgggatgca cgcgtggctg gccgcgctgc
     8461 cgtcggcgaa gggccacgcc acgcgatcgc cggctgccac gccctcgacg tcagggccga
     8521 cggcgagcac gaccccggcg ccctcacggc ccaacacgaa gggcgtcggc gtcgggtaga
     8581 tgccttcgcg ctggtagacg tcgatgaagt tgatgccggc cgcggcgacc gcgatcaagg
     8641 cctcaccggg gccgggcgtc gggatctcgc ggtcctggac ctcgagcacc tccggccccc
     8701 cggggcgggt gacgacgagg gcgcg
//
LOCUS       DAOSOC010000103         8704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_8062_length_8704_cov_9.213319, whole genome shotgun sequence.
ACCESSION   DAOSOC010000103 DAOSOC010000000
VERSION     DAOSOC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8062_length_8704_cov_9.213319"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /gene="prmC"
                     /locus_tag="PL014_08630"
     CDS             <1..877
                     /gene="prmC"
                     /locus_tag="PL014_08630"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT57194.1"
                     /translation="DLHTAVRGASARLEAAGIRSPENDAVELAAFALGTTAGDVRRAM
                     VLRTTPDAGFAAAFEALVSERLTRVPLQHLTGRAHFRGLTLAVGPGVFVPRPETEVTA
                     GLAIDAASAMGRQGDGVVVVDLCTGSGAIALAVKDELPGAEVLAVELSDLAHGWALAN
                     RDHLGLDVEVRLGDATTAFDDLEGEVDVVVSNPPYIPVGAVPVDPEVRDHDPALALYG
                     GSADGLAIPLAVAARAAVLLREGGVLVMEHADSQGASLPAALRATGLWGDVLDHADLT
                     GRPRATVAVRAGAHR"
     gene            936..1697
                     /locus_tag="PL014_08635"
     CDS             936..1697
                     /locus_tag="PL014_08635"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT57195.1"
                     /translation="MSPVFDCTTEPGRADAIPKAAAGVRAGSLVVMPTDTLYGIGADA
                     FSPDAVRDLLAAKGRGSDMPPPVLVPDARTVDGLATDVPDYARALVAAFWPGPLTIVL
                     RAQPSLMWDLGDTGGTVALRMPDDPVALELLREVGPMAVSSANRTGHPASRTVVEAAS
                     QLGAAVEFYLDGGPVTGGLASTIVDCTRDEPVILRVGALATSDVMDVVERARADALEL
                     SRPESGDEDAADASDPPAASETPTASEQRESSDDD"
     gene            1690..2982
                     /gene="glyA"
                     /locus_tag="PL014_08640"
     CDS             1690..2982
                     /gene="glyA"
                     /locus_tag="PL014_08640"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT57196.1"
                     /translation="MTDTFYGSDFGALDAFDPEIAGVLLSELDRLRGGLQLIASENFS
                     SPEVLTALGSTLSNKYAEGYPGRRYYGGCSEVDKAESLAIERCKALFGADHANVQAHS
                     GASANQAVYGAFLKPGETILAMSLPHGGHLTHGTKMSFSGKWFDAVHYGVDKDTENID
                     YDEVERLAKERRPKVILAGGSAIPRLIDFARFRAIADEVGAIFWVDAAHFIGLVAGGA
                     IPSPVPYADVVSFTTHKVLRGPRSGAIVCKAEHATAIDRAVFPMMQGGPQMHTIAAKA
                     VNFKECATPEYAAYTRQVVANAKVLAQTLGEHGIRPTTGGTDTHLSLHDLQPVQVTGV
                     DAEARADAAGITLNKNAIPFDPQSPNIASGVRVGTPCVTTQGMGEDEMRTIAELIAKA
                     VVHGDADPDHAVSREVRAQVSELVGRFPAYPRAGAAQA"
     gene            2997..4388
                     /locus_tag="PL014_08645"
     CDS             2997..4388
                     /locus_tag="PL014_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOT57197.1"
                     /translation="MSTPSPGTDRPILARLNSRGFRLVMLLDGAAILGLAVGSMWWRY
                     GLPPWPTYPTGAYAVSFAVSTAIFISAFYFGGLYEREPRLGGAAVFPRAAGLSLAAGS
                     LIALLNLALTSLANQRGSLTQRVLPFPIPNLLAVIVLGAVVVAINRAVVHALRTRREG
                     PPRVVIAGEQGDLELARRHLAAVDARALVVVAEVDDPAGVVDVARELRATDVLLVTGA
                     WLDSWYPKTIDHLDQLGLTTLLRVTGRETLFGLDRLREIGGMPFVLLHAQTMPSSQRH
                     FKRILDLVLLLVTAPVWIVLLVAVSLYQFAVAGTPVLFTQTRVGVGGAPFDMVKFRTM
                     RTDAEADGVATLSTGKADSRVIPACRWVRATRADELPQILHILTGKMSIVGPRPERPE
                     LTAGFERDIAGYTRRHEVPPGLTGLAQIHGRYHTDAEYKLGYDLQYLVNWSPVLDLEI
                     IARTIWVIVTRRV"
     assembly_gap    4499..4598
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4637..5089
                     /locus_tag="PL014_08650"
     CDS             4637..5089
                     /locus_tag="PL014_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57198.1"
                     /translation="MTDRSTSTEPHAGAPFGSMLRGALLPSLVAGALTVVALVVVRGG
                     DALAGALIGLVVSVGFFGSGMFLLSRLVRSASPHAFFAVAMAVYLGQVIALLLVILAF
                     KDAAWVDGPALGIVALVVTIVWQGFAMRALRTARVPVYDPPAADGAAR"
     gene            5205..6035
                     /gene="atpB"
                     /locus_tag="PL014_08655"
     CDS             5205..6035
                     /gene="atpB"
                     /locus_tag="PL014_08655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOT57199.1"
                     /translation="MSLSVVPAHWLQSGIAATDGEGFQAPGPESFWWPLIGSDTSNWA
                     FTRPSFVVMASVVIIAIYYMSVVNKLSIVPTKRQWIAEQSYDFVRNTIARDVIGSKDF
                     LQFLPLLLTLFTLILFNNIMGIVPFVQFPSMSRIAFPIVLTLIVYIVYHTVAIRRKHG
                     VVGYLKSLVPPGLPGWLKPIMFILEFLTYFLIRPLTLALRLFGNMLAGHLMLLVFILG
                     GEYLLLHGSNLFVQFSGVLGLGFGIVMTFFELLVQFLQAFIFTLLAALYISDAVSEHH
                     "
     gene            6120..6329
                     /gene="atpE"
                     /locus_tag="PL014_08660"
     CDS             6120..6329
                     /gene="atpE"
                     /locus_tag="PL014_08660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOT57200.1"
                     /translation="MTGDITFLGYGLAAIGPGIGVGLIFAAYINGVARQPEARGMLQT
                     IAFTGMALTEALAILGLVFVFVFAG"
     gene            6373..6957
                     /locus_tag="PL014_08665"
     CDS             6373..6957
                     /locus_tag="PL014_08665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOT57201.1"
                     /translation="MVLASVKASEGVGWPAALPLLPHPAEIIIGLIAFAILYRIFSKS
                     VVPKMEAMYAERAAAIEGGMQEAEQAQAEAQAALEKYNAQLAEARTEANEIRESAREQ
                     GASIVAEMRAQAQAEAARISESAKRQVEAERQQAVVQLRQEVGTLSTTLAGKIVGESL
                     EDEVRQKGIVDRFLADLEAGDIVPEKVAPAGQDA"
     gene            6957..7772
                     /locus_tag="PL014_08670"
     CDS             6957..7772
                     /locus_tag="PL014_08670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOT57202.1"
                     /translation="MRGSSRGSATAVQAALDAVLAQGSSWAPLADELFAVTAVVDGNA
                     SLRRALADPSRGTAEKQGLARAVFGGKVGETTTNLVAEVAGQRWSAERDLTDTLESTA
                     VQCLLATAERAGHIDRVEDELFRFERIVAGDPALRDTLSSRNTDGNGKAALVRGLLEG
                     KAAPETVRLAEQAVRAPRGRRLDRTMEAYLALASTRRDELTALVTAATPLTEQQGARL
                     RSALEAHYGKAVSLQVVLDPTVMGGIRVQVGDDVVDGTVLRRLDEARRHVTGS"
     gene            7876..>8704
                     /locus_tag="PL014_08675"
     CDS             7876..>8704
                     /locus_tag="PL014_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOT57203.1"
                     /translation="MTELSIRPDEIRDALDSYVRSYEPGAAAREEVGRVTDAGDGIAH
                     VEGLPSAMTNELLQFEDGTLGLALNLDVHEIGVVILGEFSAIEEGQEVKRTGEVLSVP
                     VGDAFLGRVVNPLGAPIDGLGEIASEERRALELQAPNVVQRKSVHEPLQTGLKAVDAM
                     TPIGRGQRQLIIGDRQTGKTTVAVDTIINQKEFWDSGDPTKQVRCIYVAIGQKGSTIA
                     AVRGTLEEAGAMEYTTIVAAPASDSAGFKYLAPYTGSAIGQHWMYQGKHVLIVFDDLS
                     "
BASE COUNT         1252 a         3171 c         2861 g         1320 t
ORIGIN      
        1 cgacctccac accgcggtcc ggggggcgtc cgcgcgcctc gaggcggccg gcatccgctc
       61 acccgagaac gatgccgtcg agctcgccgc gttcgccctc ggcaccaccg cgggcgacgt
      121 gcgccgcgcc atggtgctgc gcacgacccc ggatgccggg ttcgcggcgg cgttcgaggc
      181 gctcgtcagc gagcggctca ccagggtccc gctccagcac ctcacgggtc gggcgcactt
      241 tcgcggtctc acgctcgcgg tggggccggg ggtgttcgtc ccccgacccg agaccgaggt
      301 gacggccggg ctcgcgatcg acgcggcatc cgccatgggc cgccagggtg acggcgtggt
      361 cgtcgtcgac ctgtgcaccg gctcaggagc catcgcgctg gcggtcaagg acgagcttcc
      421 cggtgccgag gtcctcgcgg tcgagctgtc cgacctcgcc cacggctggg ccctggccaa
      481 ccgcgaccac ctcgggctcg acgtcgaggt gcgcctcggt gacgccacga cggccttcga
      541 cgacctcgag ggcgaggtcg acgtggtcgt cagcaacccg ccgtacatcc ccgtcggtgc
      601 cgtgccggtc gacccggagg tgcgcgacca cgaccctgcc ctcgcgctct acggcggctc
      661 ggccgacggg ctggcgatcc cgctggcggt cgcagcacgc gctgcggtgc tgctgcgcga
      721 gggtggggtc ctggtcatgg aacacgccga cagccagggg gcgagccttc ccgctgctct
      781 tcgcgcgacg gggctgtggg gcgacgtgct cgaccatgcc gatctcaccg gtcggccccg
      841 agcgacggtg gcggtgcgcg caggggcgca caggtgacgc gccctcggcg ggggccttca
      901 cgagccgagc ccggttcgac cctaggcttg gcctcatgag ccccgtcttc gactgcacga
      961 ccgagcccgg acgggcggat gccattccca aggccgccgc cggggtgcgc gccggcagcc
     1021 tcgtcgtgat gcccaccgac accctctacg gcatcggggc tgacgccttc tccccggatg
     1081 ccgtgcgcga cctcctcgcc gcgaagggac gggggagtga catgccgcct cccgtgctcg
     1141 tccccgatgc gcgcacggtc gacgggctcg cgacggacgt gcccgactat gcgcgagcac
     1201 ttgtcgccgc cttctggcca ggcccgctga ccatcgtcct gcgcgcccag ccgtccctga
     1261 tgtgggacct cggcgacacc ggtggcacgg tcgccctgcg gatgccggac gacccggttg
     1321 cgctggagct cttgcgcgag gtggggccga tggcggtgag cagcgcgaac cgcaccggcc
     1381 acccggcatc gcgcaccgtc gtcgaggcgg ccagccagct cggcgcggcg gtcgagttct
     1441 acctcgacgg cgggcccgtc accggtggcc tggcatcgac gatcgtcgac tgcacccgag
     1501 acgagccggt gatcctgcgg gtcggagccc ttgcgacgtc ggacgtcatg gacgtcgtcg
     1561 agcgtgcgcg cgccgacgcc ctagagttgt cgcgcccgga gtcgggcgac gaggacgcgg
     1621 cggacgcgtc cgatccgccc gccgcgagcg aaacccccac cgccagtgag cagcgagaga
     1681 gcagcgacga tgactgacac gttctacgga tccgacttcg gcgccctcga cgccttcgac
     1741 cccgagatcg cgggggtgct gctgagcgag ctcgaccggc tgcgcggcgg cctccagttg
     1801 atcgcgagcg agaacttctc cagccccgag gtgctgacgg cactcggttc gaccctgtcg
     1861 aacaagtacg ccgagggata cccggggcgc cggtactacg gcggctgttc cgaggtcgac
     1921 aaggccgagt cgctcgcgat cgagcgctgc aaggcgctgt tcggcgccga ccacgccaac
     1981 gtccaggccc actccggcgc gagcgcgaac caggccgtct acggcgcgtt cctcaagccc
     2041 ggcgagacga tcctcgcgat gagcctgccg cacggcgggc acctcaccca cggcacgaag
     2101 atgtccttct ccggcaagtg gttcgacgcg gtgcactacg gagtcgacaa ggacaccgag
     2161 aacatcgact acgacgaggt cgagcgcctg gccaaggaac gccgccccaa ggtgatcctc
     2221 gcgggcggtt cggccatccc gcggctcatc gacttcgccc ggttccgcgc catcgccgac
     2281 gaggtcgggg cgatcttctg ggtcgatgca gcacacttca tcggcctcgt cgccggcggt
     2341 gccatcccca gcccggtccc ctacgcggac gtcgtgtcgt tcacgacgca caaggtgctg
     2401 cgcggcccgc gctcgggagc catcgtctgc aaggccgagc acgccaccgc catcgaccgg
     2461 gccgtcttcc cgatgatgca gggcgggccg cagatgcaca cgatcgccgc caaggcggtc
     2521 aacttcaagg agtgtgcgac gcctgagtac gcggcgtaca cccgccaggt cgtcgccaac
     2581 gccaaggtcc tcgcgcagac gctgggggag cacggcatcc gcccgacgac ggggggcacg
     2641 gacacccacc tgtcgctgca tgacctccag ccggttcagg tgaccggcgt cgacgccgag
     2701 gcacgcgcgg acgccgccgg gatcacgttg aacaagaacg ccattccctt cgacccgcag
     2761 tcgccgaaca tcgcctccgg ggtgcgcgtc ggcacgccgt gcgtcacgac ccagggcatg
     2821 ggtgaggacg agatgcgcac gatcgccgaa ctgatcgcca aggccgtcgt gcacggtgac
     2881 gccgacccgg accacgcggt ctcacgcgag gtgcgggccc aggtgagcga gctcgtcggg
     2941 cgtttcccgg cctacccgcg cgcgggtgcg gcacaggcct gagacctcgg atcttcgtgt
     3001 ccacaccgag ccccggcacc gaccggccga tcctggcccg gctcaacagc cgtgggttcc
     3061 ggctcgtcat gctgctggac ggcgccgcca tcctcggcct ggccgtgggg tcgatgtggt
     3121 ggcgctacgg gctgccaccc tggccgacgt acccgacggg tgcctatgcg gtgtcgttcg
     3181 ccgtctcgac ggcgatcttc atctcggcgt tctacttcgg cgggctctac gaacgtgagc
     3241 cgcggctggg cggtgccgcc gtgttcccac gggctgccgg gttgtcgctg gcagccggca
     3301 gcctcatcgc gctgctcaac ctcgccctca cgagtctggc caaccagcgg ggctcactga
     3361 cccaacgggt cctgccgttc ccgatcccca acctgctcgc cgtcatcgtg ctcggcgctg
     3421 tcgtcgtggc gatcaaccgc gcggtcgtgc acgccctgcg gacgcgtcgt gagggccctc
     3481 cgcgcgtggt catcgccggc gagcagggtg acctcgagct cgcccggagg cacctggctg
     3541 ccgtcgacgc gagggcgctg gtcgtcgtcg cggaggtcga tgacccggct ggcgtcgtcg
     3601 acgtcgcccg tgagctgcgg gccaccgacg tcctgctcgt caccggcgcg tggctggaca
     3661 gctggtaccc gaagaccatc gaccatctcg accagctggg cctgaccacc ctgttgcggg
     3721 tgaccggtcg cgagacgctg ttcggtctcg atcggctgcg cgagatcggg gggatgccgt
     3781 tcgtcctcct ccacgcgcag accatgccga gctcgcagcg gcacttcaag cgaatcctcg
     3841 acctcgtgct gctcctcgtc accgctccgg tgtggatcgt cctgctcgtg gcggtgtcgc
     3901 tctaccagtt cgcggtggcc gggacgccgg tgctcttcac ccagacccgg gtgggcgtgg
     3961 gtggggcacc gttcgacatg gtcaagttcc gcacgatgcg caccgatgcc gaggccgacg
     4021 gtgtggcgac gctctcgacc ggcaaggccg actcgcgggt catcccggcc tgccgttggg
     4081 tgcgggcgac ccgggccgac gagctgcccc agatcctgca catcctcacg ggcaagatgt
     4141 ccatcgtcgg gccgcgcccc gagcgtcctg agctgaccgc cggcttcgag cgggacatcg
     4201 cggggtacac ccgccgtcac gaggtcccgc ccgggctgac cggcctggcc cagatccacg
     4261 gtcggtacca caccgacgcc gagtacaagc tgggctacga cctccagtac ctcgtcaact
     4321 ggtcgccggt gctcgacctc gagatcatcg cccgcaccat ctgggtcatc gtcacgcgac
     4381 gggtctgagc gcccttttgg gctgtacccg tcgcttgtga taactttcac aagccgtccc
     4441 ggagacctat ccgtcacgac ccccgaagag gccgatcgcc gatgaccgac cgctccacnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga cctatccgtc acgacccccg
     4621 aagaggccga tcgccgatga ccgaccgctc caccagcacc gagccgcacg ccggcgcccc
     4681 cttcggttcc atgctccgcg gggccctgct gccctcgctg gtcgccgggg cgctgacggt
     4741 cgtggccctg gtcgtcgtgc gtgggggaga cgccctggcc ggtgcgctca tcggcctcgt
     4801 cgtctcggtg ggcttcttcg gttccggcat gttcctgctg agcaggctcg tgcgcagcgc
     4861 cagcccgcac gcgttcttcg ccgtcgccat ggcggtctac ctcggccagg tcatcgccct
     4921 gctgctcgtc atcctggcgt tcaaggacgc cgcgtgggtc gatgggccag ccctgggcat
     4981 cgtggccctg gtcgtgacga tcgtgtggca ggggttcgcg atgcgcgccc tgcgcaccgc
     5041 acgtgttccc gtgtacgacc cgcccgcggc cgacggcgcc gcacggtgag acgcatcgaa
     5101 acccccacga cctgcgtcgc ggccaccctc gcgggtccgg tagcgtacgt cccgttcccc
     5161 cgggtgcccg tcgatggcgc ctgtgcaacc cccgaggaga ggctgtgagc ctgagcgtgg
     5221 ttcccgcaca ctggcttcag agcggtatcg ccgcaaccga cggcgagggc ttccaggccc
     5281 ccggccccga gtccttctgg tggccgctga tcggcagcga cacgagcaac tgggcgttca
     5341 cccgtccctc gttcgtcgtc atggcctctg tcgtgatcat cgcgatctac tacatgagcg
     5401 tggtcaacaa gctgagcatc gtgccgacca agcgccagtg gatcgccgag cagagctacg
     5461 acttcgtgcg caacaccatc gcccgcgacg tcatcgggtc gaaggacttc ctgcagttcc
     5521 tgccgctgct gctgacgctg ttcacgctca tcctgttcaa caacatcatg gggatcgtgc
     5581 cgttcgtgca gttcccctcg atgagccgga tcgcgttccc gatcgtgctg accctgatcg
     5641 tctacatcgt ctaccacacc gtggcgatcc gccggaagca cggcgtcgtc ggctacctca
     5701 agtcgctggt gccccccggg ctgcccggct ggctgaagcc gatcatgttc atcctggagt
     5761 tcctgaccta cttcctcatc cggccgctga cgctcgccct gcgactcttc ggcaacatgc
     5821 tcgccggcca cctcatgctg ctcgtcttca tcctcggcgg cgagtacctg ttgctgcacg
     5881 gcagcaacct gttcgtccag ttctccggtg tgctcggcct cggcttcggc atcgtcatga
     5941 ccttcttcga gctcctggtg cagttcctcc aggcgttcat cttcaccctg ctcgccgcgc
     6001 tctacatctc cgacgcggtg agcgagcacc actgatctcc cggacccgtc cggtaagtac
     6061 aaccacctga tgaacactcc gacgccggga gcccctggcg ccaccgaaag gaaaacgcaa
     6121 tgactggtga catcaccttc ctcggctacg gcctcgccgc catcggcccc ggtatcggtg
     6181 tgggtctcat cttcgccgcc tacatcaacg gcgtcgctcg ccagcccgag gcccgaggca
     6241 tgctccagac catcgccttc accggtatgg ccctcaccga ggcgctcgcg atcctcggtc
     6301 tcgtgttcgt cttcgtcttc gcaggctgac gcaccgacga ccccccgttc gatcgacaga
     6361 taaggaagcg acgtggttct cgcatccgtg aaggcatccg agggtgtcgg ctggccggca
     6421 gcacttccgt tgctgccgca ccccgccgaa atcatcatcg ggctgatcgc cttcgccatc
     6481 ctctacagga tcttctccaa gagcgtcgtc cccaagatgg aggcgatgta cgccgagcgt
     6541 gccgcagcca tcgagggcgg catgcaggag gccgagcagg cgcaggccga ggcccaggct
     6601 gccctggaga agtacaacgc ccagctcgcc gaggctcgca ccgaggccaa cgagatccgc
     6661 gagtccgctc gtgagcaggg cgcgtcgatc gtcgcggaga tgcgtgccca ggcccaggcc
     6721 gaggcagccc gcatctccga gtcggccaag cgtcaggtcg aggccgagcg ccagcaggcc
     6781 gtggtccagc tgcgccagga ggtcggcacg ctgtcgacga ccctggcggg caagatcgtc
     6841 ggcgagtcgc tcgaggacga ggtgcgccag aagggcatcg tcgaccggtt cctcgccgat
     6901 ctcgaggccg gcgacatcgt ccccgagaag gttgccccgg cggggcagga cgcctgatgc
     6961 gcggctcgtc gcgaggttcg gccactgcgg tgcaggcggc gctcgacgcc gtgctggcgc
     7021 aggggtcgtc ctgggcaccg ctcgcggatg agctcttcgc cgtgaccgcg gtcgtcgacg
     7081 gcaacgcgtc gctgcgtcgg gcccttgcgg acccctcgcg cggcaccgcc gagaagcagg
     7141 gcctggcccg tgcggtcttc gggggcaagg tcggtgagac caccaccaac ctggtcgccg
     7201 aggtcgcagg gcagcgctgg tccgccgagc gtgacctcac ggacacgctc gagtccacag
     7261 ccgtgcagtg cctgctggcc acggccgagc gagccggtca catcgaccgg gtcgaggacg
     7321 agctcttccg cttcgagcgg atcgtcgccg gcgaccccgc tctgcgcgac acgctctcca
     7381 gccgcaacac cgacggcaac ggcaaggccg ccctcgtgcg gggtctgctc gaaggcaagg
     7441 cggcgcccga gacggtccgc ctcgcggagc aggcggtgcg cgccccccgc ggacgccgtc
     7501 tggaccgcac catggaggcc tacctcgcgt tggcctcgac ccgccgtgac gagctcaccg
     7561 cgctcgtcac cgcggcgacg cccctcaccg agcagcaggg tgcccggctg cgcagcgccc
     7621 tcgaggcgca ctacggcaag gccgtcagcc tccaggtcgt cctcgacccc accgtcatgg
     7681 gcggcatccg ggtccaggtc ggcgacgacg tcgtcgacgg caccgtcctg cggcgcctcg
     7741 acgaagcccg ccgccacgtg accggcagct gacaccgcac gtccacgcac ggcatccgaa
     7801 caccgcaaga caccgcacca ccgaccacag acttcggccc caccaggggc cggtgaccga
     7861 ggagaagatc agaacatgac ggagctctcg atccgtccgg acgagatccg ggacgccctg
     7921 gacagctacg tccggtccta cgagcccggc gcagccgcgc gcgaagaggt cggtcgcgtc
     7981 accgacgccg gcgacggcat cgcgcacgtc gagggcctgc cctcggcgat gaccaacgag
     8041 ctcctccagt tcgaggacgg caccctgggc ctggcgctca acctcgacgt ccacgagatc
     8101 ggcgtcgtca tcctcggtga gttctccgcg atcgaggagg gccaggaggt caagcggacc
     8161 ggcgaggtgc tctccgtgcc cgtgggcgac gcgttcctcg gccgtgtcgt gaacccgctc
     8221 ggtgcgccga tcgacggcct cggcgagatc gccagcgagg agcgccgcgc cctggagctc
     8281 caggcgccca acgtcgtgca gcgcaagtcg gtgcacgagc cgctgcagac cggcctgaag
     8341 gccgtcgacg ccatgacgcc gatcggccgt ggccagcgcc agctcatcat cggtgaccgc
     8401 cagaccggca agaccacggt cgcggtcgac acgatcatca accagaagga gttctgggac
     8461 agcggcgacc cgacgaagca ggtgcgctgc atctacgtcg ccatcggcca gaagggctcc
     8521 acgatcgccg ccgtccgcgg cacgctcgag gaggcgggcg cgatggagta caccaccatc
     8581 gtcgccgctc cggcctccga ctccgcgggc ttcaagtacc tcgccccgta caccggctcg
     8641 gccatcggcc agcactggat gtaccagggc aagcacgtcc tcatcgtctt cgacgacctg
     8701 tcca
//
LOCUS       DAOSOC010000104         8592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_8177_length_8592_cov_8.434930, whole genome shotgun sequence.
ACCESSION   DAOSOC010000104 DAOSOC010000000
VERSION     DAOSOC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8177_length_8592_cov_8.434930"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PL014_08680"
     CDS             complement(<1..645)
                     /locus_tag="PL014_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57204.1"
                     /translation="MTPIQALRRGASRRVELVPMNPIEQLRAGRLPHRVAQLLVGLVL
                     FGISMALLLRADLGLEPWGVLQYGLIQHIPMSYGLMSIVVSFVVLLLWIPLRQWPGLG
                     TVLNAVVIGLAIDATLAVVPDIEGLGWRVAVMLLAIVGNGVAGAMYIGSQLGPGPRDG
                     LMTGLARRTGRSIRLVRTSLEVTVVVLGFALGGILGVGTVLYALLIGPVVQLFLP"
     gene            709..>2148
                     /locus_tag="PL014_08685"
     CDS             709..>2148
                     /locus_tag="PL014_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT57205.1"
                     /translation="MSTPTVSAARTARIVGTLTPGRPAYRALADALRLAIADGRIAAG
                     TRLPSERELTASLDVSRTTVTRAYAVLRDTGYLTSRRGSGSVASLPSGSRVRGAGSLF
                     PADPGEGVIDLTCAATRAPAGVVEAYERAVSQLPEHLAGAGYLTLGIPDLRAVIADRY
                     TQRGLPTSPDDVLVTSGAVAGIGIVLRALLGHGDRVVVESPTYPNTIDALRRSGARLV
                     PLAIDPDGWDATESARTIRAAGASLAVLIPDFHNPTGALMGDDARATLARALTASSTI
                     PVIDETIVEVDLDDADPMPLPFAAHDPRAICVGSSSKSHWGGLRTGWIRAPKGTLPAL
                     VEARVTTDLGAPVLEQLVLLELMRAQPGLSPQRRLDLRASRGALADALREHLPQISFR
                     MPRGGLSLWCELPEDVDATSVAVAAEDAGLLVAAGPRFAVVGGLDRWIRVPFVLPPEV
                     MVDAVARLAGALESARAPLASGRVAAPAAN"
     assembly_gap    2149..2248
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2249..3223
                     /locus_tag="PL014_08690"
     CDS             <2249..3223
                     /locus_tag="PL014_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HOT57206.1"
                     /translation="QPAPLLDLLATLDLEELGSAVMSVQPLSTADTADGTLPTPLPET
                     FAEDITLFRGQSQKQPHGRVFGGQVLAQGVIACGRTVRVIEGGEKRRLHSLHAYFMRP
                     GDDTQPITFSVERMRDGRSFSTRRVHAIQHGKPILSMSASFQDPDAGLDHQDPFPQTP
                     SPDTLPSLAELFEGVDHPAARHMAEGRAIDQRHVEGNLFATPATDRVADQRVWMRATG
                     TLPDDPLIHDAVLAYASDYSLLETILRRHGRTWTTPGLRAASLDHAMWFHRPARADEW
                     ILYAEHSPSAQGGRGLGIGRMFTEGGILVATVAQEGMLRVPSPEDDRG"
     gene            complement(3200..5119)
                     /gene="treZ"
                     /locus_tag="PL014_08695"
     CDS             complement(3200..5119)
                     /gene="treZ"
                     /locus_tag="PL014_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOT57207.1"
                     /translation="MLPIEVWAPKAEHVEIEWSVPESTNRSLGVQPADGAGVPESTNR
                     SLGVQPRRESMTALGDGWWRWDGPSTASESDGTPTASDLVLDYAFVLDGAEPPVPDPR
                     SAWQPHGVHAPSRTFDPATLTWTDGDWRGPRDGQGVLGGVVYELHIGTFTARGTFDAA
                     IERLDHLVDLGVDVVNVMPIAAFDGRWGWGYDGVGLYAVHDPYGGPAAFARFVDACHA
                     RGLGVCLDVVHNHLGASGNYLARFGPYFTTAHSTPWGPAVNLDQDHSDHVRGFIVDNA
                     LRWLRDFHVDALRLDAVHELRDASPRHLLADLSDAVAALAEHLARPLDLIAESDLNDV
                     DMITPTAQGGRGMSAQWDDDIHHALHVTLTGESHGYYTDFAGGAGRDEAGPLAVLAKV
                     LTCGFLHDGSHSSFRGRPWGQPVDTEHVDARRLLGYLQTHDQVGNRMTGDRISAVVPP
                     GLQAAGAALYLLAPTTPMIFMGEEWGASTPWQYFTSFTDEALADAVRAGRRAEFASHG
                     WSTADVPDPQNPATREASVLAWGEVDEAGHRRLLDWYRACIALRRRVIGVGPTRLADV
                     RVDVDEAARTVVTLHAPAGRTASAVVLNLADDPADVPLPRSGGRVALAWDPEGTTLTT
                     DAVRLPGGSAAVVLW"
     gene            complement(5146..7620)
                     /gene="treY"
                     /locus_tag="PL014_08700"
     CDS             complement(5146..7620)
                     /gene="treY"
                     /locus_tag="PL014_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HOT57208.1"
                     /translation="MSDASTASAHRPNPARVVPTSTYRLQIHEGFTYDDAAGHTEYLA
                     SLGVSHVYLSPVLQAAPGSMHGYDVLDHTRISEAAGGRGGFERLVAACRAAGLGIIVD
                     VVPNHMAVPQPAHLNAPFWSLLREGRRSPYAGWFDVDWVAEDDRLLMPVLGGTLQQAL
                     EAGELVLAADGGPTASDPVLRYYDAEFPVAPGTEGLPLAELVDAQAYRLSSWREAGEA
                     LNYRRFFDVTSLVAVRVEDPVVFMATHALLLALHGHGAVDGFRIDHPDGLADPGGYLD
                     RLADATGDSWVVVEKILEGDEQLPDSWRCAGTTGYDALLRVQQVLTPSAGAAVLDRLW
                     AQAAPDRGVLDDVVIEAKRLVVDDVQAAEVDRLMRLVRRILPDLDQASARRALEALLV
                     AMDRYRAYVVPGRAVDPEQAAVIEAAAGRARTHLAPTDEPALGVIVDLVLDRDLRDAA
                     LDTGPLADDFRVRFQQTCGPVMAKGIEDTAYYRWFRLAGANEVGGHPDHLSISAEEFH
                     TWCGRLVASWPTSMTTLTTHDTKRSEDVRARLMSLAEDGEGWATWVAGARELGDAHRP
                     ALVDAPTEYLLWQTLVGAWPITGSRLEGYLLKAVREAKVHTAWVDGDTDYEDAVTSYA
                     RDLTHDPLVQSHLDAWTVAHEPSVRGNVLAQKLIQLTMPGVPDVYQGCETVTLSLVDP
                     DNRRPVDWTDRGERLARVVAAEPALDLDDEKMRVTARALRVRREDPAAFVGEGTTYTG
                     LPCSSPHALAFARGDADGLRVVTVATVAAGTLAAGGGFGDAVVELPPGSWRDVLADGD
                     DVITGGSVSLATLLAHRPVALLVRHS"
     gene            complement(7626..>8592)
                     /locus_tag="PL014_08705"
     CDS             complement(7626..>8592)
                     /locus_tag="PL014_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HOT57209.1"
                     /translation="WTEWNGKYRDTVRDFWRGEPRTLGEFASRLTGSSDLYEHSGRKP
                     IASINFVTAHDGFTLRDLVSYNDKRNEANGEDGNDGESHNRSWNCGEEGPSENPDVIA
                     LRLRQQRNFLTTLLLSQGVPMIAHGDEIGRTQQGNNNVYCQDNELAWVDWDLDEAQKE
                     LYAFTAALTTLRREHAVFRRRRFFAGSADHGGTSELGDIAWFQPSGELMGESAWANDE
                     AKTVMVFLNGQAIPSMDSRGRRVLDDDFLVIFNAHHEADSFVLPDAEWGEHWVAEIDT
                     GADVVDQAWHDAGSELVVAPRSVIVLRSPREAPPQPSGAATATRV"
BASE COUNT         1198 a         3132 c         3019 g         1143 t
ORIGIN      
        1 cggcaggaag agctgcacga ccggcccgat gagcagggcg tacagcaccg tccccacgcc
       61 gaggatgccg ccgagggcaa acccgagcac gacgaccgtc acctccagcg aggtgcgcac
      121 caggcggatc gagcgcccgg tgcgccgcgc cagcccggtc atcagcccgt cccggggccc
      181 cgggccgagc tggctgccga tgtacatcgc cccggccacc ccgttgccga cgatcgccag
      241 gagcatcacg gccacccgcc accccagccc ctcgatgtcg ggcaccacgg ccagcgtcgc
      301 gtcgatcgcg agaccgatga ccacggcgtt cagcaccgtc cccagccccg gccactgacg
      361 cagcgggatc cacagcagca gcacgacgaa cgacacgacg atgctcatga ggccgtacga
      421 catggggatg tgctggatca gtccgtactg caacaccccc cacggctcga gccccaggtc
      481 ggcgcgcagc aacaaggcca tcgagatgcc gaacagcacg agaccgacga gcagctgggc
      541 cactcggtgc ggcaggcgac cagcgcgcag ctgctcgatc gggttcatcg ggacgagctc
      601 gacacgacgg ctcgcgccgc gacggagggc ctggatcggg gtcatgcgct ccagcatgcc
      661 ccagaagtgg ccttcacttc gatagccaat cgtgcaagag tggccatcat gtcgacgccc
      721 accgtctccg cagcccgcac cgcacggatc gtcggcaccc tcacccccgg acgccccgcc
      781 taccgggccc tggccgacgc cctgcgcctg gccatcgccg acggccgcat cgccgctggc
      841 acccggctgc cgagcgagcg cgagctcacg gcatccctcg acgtcagccg cacgaccgtg
      901 acccgggcgt atgccgtgct gcgcgacacc ggctacctca cctcgcgccg tggctcgggg
      961 tccgtggcct cgctcccctc cggcagccgg gtccgcggcg ccggatcgct cttccccgcc
     1021 gacccgggcg agggtgtcat cgacctcacc tgcgcggcca cccgggcacc cgcgggcgtc
     1081 gtcgaggcct acgagcgagc ggtgtcccag ctccccgaac acctggccgg ggccggctac
     1141 ctgaccctcg gcatccccga cctgcgcgcg gtcatcgccg accgctacac ccagcgcggc
     1201 ctgccgacca gccccgacga cgtcctcgtc acctcggggg cggtcgccgg catcggcatc
     1261 gtgctgcggg cgttgctcgg ccacggcgac cgcgtcgtcg tcgagagccc cacctacccc
     1321 aacaccatcg acgccctgcg ccgctcgggt gcccgtctcg tcccgctggc gatcgacccc
     1381 gacggctggg acgccaccga gtcggcccgc accatccgtg ccgccggcgc gagcctggcc
     1441 gtgctcatcc ccgacttcca caaccccacc ggcgccctga tgggcgacga cgcccgcgcc
     1501 acgctcgccc gcgcgctcac ggcatcctcg acgatccccg tcatcgacga gacgatcgtc
     1561 gaggtcgacc tcgacgacgc ggacccgatg ccgctgccct tcgccgcgca cgacccgcgg
     1621 gcgatctgtg tcggcagctc gtcgaagtcg cactggggtg gcctgcgcac cgggtggatc
     1681 cgggcaccga agggcacctt gcctgcgctc gtcgaggccc gggtgaccac cgacctcgga
     1741 gcccccgtgc tcgaacagct cgtgctcctg gagctgatgc gcgcccaacc cggcctgtca
     1801 ccgcaacgcc gcctcgacct gcgcgcctcg cggggcgccc tcgccgacgc gctgcgagaa
     1861 cacctcccgc agatctcttt ccggatgccg cgcggcgggc tgtcgctgtg gtgcgagctg
     1921 cccgaggacg tcgacgccac ctccgtcgcc gtggccgccg aggatgccgg gctgctcgtc
     1981 gccgccggcc cgcgcttcgc ggtcgtcgga gggctggatc gctggatccg ggtgccgttc
     2041 gtcctgccgc ccgaggtcat ggtggatgcc gtggctcgcc tcgccggtgc cctcgagtcc
     2101 gcgcgcgccc cccttgcgtc cggcagggtg gctgccccag cagccaacnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gcccgcaccg ctcctcgacc tcctggccac
     2281 cctggacctc gaggagctcg gctcggcggt gatgagcgtg cagcccctgt cgacggccga
     2341 caccgccgac ggcacgctgc cgacccccct gcccgagacc ttcgccgagg acatcaccct
     2401 gttccgcggg cagagccaga agcagccgca cggtcgggtg ttcggcgggc aggtcctcgc
     2461 ccagggcgtc atcgcctgtg gccgcaccgt gcgcgtcatc gagggcggcg agaagcggcg
     2521 cctgcactca ctgcacgcgt acttcatgcg ccccggcgac gacacccagc cgatcacgtt
     2581 cagcgtcgag cgcatgcgtg acggccgctc cttctcgacc cgccgggtgc acgccatcca
     2641 gcacggcaag ccgatcctgt cgatgagcgc ctcgttccag gaccccgacg ccggcctcga
     2701 ccaccaggac ccgttcccgc agaccccctc ccccgacacg ctcccgagcc tggccgagct
     2761 cttcgagggc gtcgaccacc cggcggcccg gcacatggcc gagggccggg ccatcgacca
     2821 gcggcacgtc gagggcaacc tcttcgcgac cccggccacc gaccgggtcg ctgaccagcg
     2881 ggtctggatg cgcgccaccg gcaccctgcc ggacgacccg ctcatccacg acgccgtgct
     2941 ggcctacgcc tccgactact cgctgctcga gacgatcctg cgccgccacg gccgcacctg
     3001 gaccacgccg ggcctgcggg cagccagcct cgaccacgcc atgtggttcc accgcccggc
     3061 ccgcgccgac gagtggatcc tctacgccga gcactccccc tcggcgcagg gcgggcgggg
     3121 actgggcatc ggccgcatgt tcaccgaagg cgggatcctc gtggccaccg tcgcgcagga
     3181 gggcatgttg cgcgtgccct caccagagga cgaccgcggc tgacccgcca ggcaggcgca
     3241 cggcatccgt ggtcagcgtg gtgccttcag ggtcccaggc gagggccacc cgaccgcctg
     3301 atcgcggcag cggcacgtcg gcggggtcgt cggcgaggtt gagcacgacg gcggatgccg
     3361 tccgcccagc aggcgcgtgg agcgtcacca ccgtgcgcgc ggcctcgtcc acgtcgacgc
     3421 gaacgtcggc gaggcgggtc gggcccacac cgatgaccct ccggcgcagg gcgatgcacg
     3481 cccggtacca gtcgagcagg cgccggtgcc ctgcctcgtc gacctcgccc caggcgagca
     3541 ccgaggcctc ccgcgtggcg gggttctgcg ggtcgggcac gtcggcggtg gaccagccgt
     3601 ggctggcgaa ctcggcccgc ctgccggcgc gcacggcatc cgcgagggcc tcgtcggtga
     3661 agctcgtgaa gtactgccac ggcgtcgagg caccccactc ctcccccatg aagatcatcg
     3721 gggtggtggg cgcgagcagg tagagcgcgg cgccggcggc ctggagcccg ggcggcacga
     3781 cggcgctgat ccggtcgccg gtcatccggt tgccgacctg gtcgtgggtc tggaggtacc
     3841 ccagcagtcg gcgggcatcg acgtgctcgg tgtcgaccgg ctggccccac gggcgccccc
     3901 ggaagctgga gtggctgccg tcgtgcagga acccgcaggt cagcaccttc gcgagcacgg
     3961 cgagtggccc agcctcgtca cgacccgccc cgccggcgaa gtcggtgtag tacccgtggc
     4021 tctcgccggt gagggtgacg tgcagggcgt ggtggatgtc gtcgtcccac tgggcgctca
     4081 tgccacgccc gccctgggcg gtcggcgtga tcatgtcgac gtcgttgagg tcgctctcgg
     4141 cgatgaggtc gagagggcga gcgaggtgct cagccagcgc cgccacggca tccgagagat
     4201 cggcgagcag gtgccggggt gaggcgtcgc gcagctcgtg cacggcatcc aggcgcaggg
     4261 cgtcgacgtg gaagtcgcgc aaccagcgca gcgcgttgtc gacgatgaag ccgcgcacgt
     4321 ggtcggagtg gtcctggtcg aggttgaccg ccggccccca gggggtgctg tgcgcggtcg
     4381 tgaagtacgg gccgaagcgg gcgaggtagt tgccgctcgc gccgaggtgg ttgtgcacga
     4441 cgtcgaggca gaccccgagc ccgcgggcgt ggcaggcgtc gacgaagcgg gcgaacgcgg
     4501 ccgggccgcc gtaggggtcg tgcacggcgt agagcccgac gccgtcgtag ccccagcccc
     4561 accggccgtc gaaggcggcg atcggcatga cgttgacgac gtcgacgccg aggtcgacga
     4621 ggtgatcgag ccgctcgatg gcggcatcga aggtgccccg cgcggtgaag gtgccgatgt
     4681 gcagttcgta gacgacgcca ccgagcacgc cctggccgtc cctcgggccg cgccagtcgc
     4741 cgtcggtcca cgtcagggtg gccgggtcga acgtgcggct cggtgcgtgt acgccgtggg
     4801 gctgccacgc gctgcgaggg tcgggcacgg gaggttcggc accgtcgagc acgaacgcgt
     4861 agtcgaggac gaggtcggat gccgtcggcg tcccgtcgct ctcggatgcc gtggacggcc
     4921 cgtcccagcg ccaccagccg tcaccgagtg cagtcatcga ctcccgtcga ggctgtaccc
     4981 cgagtgacct attggtcgat tcgggtacgc cagccccgtc ggcaggctgt accccgagtg
     5041 acctattggt cgattccggt acggaccact cgatctcgac gtgctcggcc ttcggggccc
     5101 agacctcgat cggcagcatg cccgcaccag gtcggctcgc cacgctcacg agtgccgcac
     5161 cagcagggcc accgggcggt gggccagcag cgtcgccagc gacaccgagc ccccggtgat
     5221 gacgtcgtcg ccgtcagcca gcacgtcgcg ccacgatccg ggcggaagct cgacgacggc
     5281 atccccgaat ccaccaccag cggcaagcgt gccggcggca acggtcgcga ccgtgaccac
     5341 gcgcagcccg tcggcatcac cgcgggcgaa ggccagggcg tgcgggctcg agcacggcaa
     5401 cccggtgtac gtcgtgccct cacccacgaa ggcagcgggg tcctcgcggc gcacgcgcag
     5461 cgcgcgggcg gtcacccgca tcttctcgtc gtcgaggtcg agcgcaggct cggcggcgac
     5521 gacgcgggcc agccgttcgc cgcggtccgt ccagtccacg ggccgacggt tgtcggggtc
     5581 gacgagcgag agcgtgacgg tctcgcagcc ctggtagacg tcgggcacgc cgggcatggt
     5641 cagctggatg agcttctgcg ccagcacgtt gccacgcacc gagggttcgt gggccacggt
     5701 ccaggcgtcc aggtggctct ggaccaaggg gtcgtgggtg aggtcgcgcg cgtaggaggt
     5761 gacggcatcc tcgtagtcgg tgtcgccgtc gacccacgcc gtgtgcacct tggcctcacg
     5821 cacggccttg agcaggtagc cctcgagccg agagccggtg atcggccagg caccgaccag
     5881 ggtctgccac agcaggtact cggtgggcgc gtcgacgagc gccggacggt gggcgtcccc
     5941 cagctcacga gccccagcaa cccacgtcgc ccatccctcg ccgtcctcgg cgagcgacat
     6001 cagccgggcg cgcacgtcct ccgagcgctt ggtgtcgtgg gtggtcagcg tcgtcatcga
     6061 cgtcggccac gacgcgacga gccggccgca ccaggtgtgg aactcctcgg ccgagatcga
     6121 caggtggtca gggtgtccgc cgacctcgtt ggcgccggcg aggcggaacc agcggtagta
     6181 ggcggtgtcc tcgatgccct tggccatgac cgggccacac gtctgctgga agcgcacccg
     6241 gaagtcgtcg gcgagcgggc cggtgtcgag tgcggcgtcg cgcaggtcgc ggtcgagcac
     6301 gaggtcgacg atcacgccga gcgcgggctc gtcggtcgga gccaggtggg tgcgtgcgcg
     6361 accagccgca gcctcgatga ccgccgcctg ctcggggtcc acggcacgcc cgggcacgac
     6421 gtaggcgcgg tagcggtcca tcgcgacgag cagggcctcg agcgcgcgcc gggccgacgc
     6481 ctggtcgagg tcgggcagga tgcgccgcac cagccgcatg agccggtcga cctcggccgc
     6541 ctggacgtcg tcgacgacca ggcgcttggc ctcgatgacc acgtcgtcga gaacaccccg
     6601 gtcgggcgcg gcctgcgccc acagccggtc gagcaccgca gcccccgccg agggggtgag
     6661 cacctgctgc acccgcagca acgcgtcgta gccggtcgtg ccggcgcacc tccacgagtc
     6721 gggcagctgc tcgtcgccct cgaggatctt ctcgacgacg acccaggagt cccccgtggc
     6781 gtcagcgagc cggtcgaggt agccgccggg gtcggcgagc ccgtcggggt ggtcgatgcg
     6841 gaagccgtcg accgcgccgt gcccgtgcag ggcgagcagc agggcgtggg tggccatgaa
     6901 cacgacgggg tcctcgacgc gcaccgcgac gagcgaggtg acgtcgaaga agcgtcggta
     6961 gttcagcgcc tcccccgcct cgcgccagct cgacaggcgg tacgcctgcg cgtcgacgag
     7021 ctcggccagc ggcagcccct cggtgccggg cgcaacgggg aactcggcgt cgtagtagcg
     7081 caggaccggg tcggatgccg tcggcccacc gtccgcggcg agcaccagtt cgccggcctc
     7141 gagggcctgc tggagcgtcc cgccgagcac cggcatcagg agccggtcgt cctcggcgac
     7201 ccagtcgacg tcgaaccacc cggcatacgg cgagcggcgg ccctcccgca acaacgacca
     7261 gaacggggcg ttgaggtggg ccggctgcgg caccgccatg tggttgggca cgacgtcgac
     7321 gatgatgccg agccccgcgg cgcggcacgc ggcgaccaga cgctcgaagc caccccggcc
     7381 accggccgct tcactgatcc gggtgtggtc gagcacgtcg tagccgtgca tcgagcccgg
     7441 cgccgcctgg agcacgggtg agaggtagac gtgcgagaca ccgagcgagg ccaggtactc
     7501 ggtgtgcccg gcagcgtcgt cgtaggtgaa cccctcgtgg atctgcaggc ggtaggtcga
     7561 cgtcggcacc acccgcgccg ggttcggccg gtgcgcggat gccgtgctcg catcgctcac
     7621 gccgatcaca cccgggtggc ggtcgcggct ccggaaggct gcggaggcgc ctcgcgcggg
     7681 ctgcgcagga cgatcaccga ccgcggcgcc acgaccagtt cgctgcccgc gtcgtgccag
     7741 gcctggtcga cgacgtcggc gccggtgtcg atctcggcga cccagtgctc accccactcg
     7801 gcgtcgggga gcacgaacga gtcggcctca tggtgggcgt tgaagatgac gaggaagtcg
     7861 tcgtcgagca cccggcgacc acgcgagtcc atgctgggga tcgcctggcc gttgaggaag
     7921 accatgacgg tcttggcctc gtcattggcc caggccgact cccccatcag ctcacccgag
     7981 ggctggaacc aggcgatgtc gccgagttcg gaggtgccgc cgtggtcggc gctaccggcg
     8041 aagaaccggc gccggcggaa caccgcgtgc tcgcggcgca gggtggtgag tgccgcggtg
     8101 aaggcgtaga gctccttctg ggcctcgtcg aggtcccagt cgacccaggc gagctcgttg
     8161 tcctggcagt acacgttgtt gttgccctgc tgggtgcggc cgatctcgtc gccgtgcgcg
     8221 atcatcggca cgccctggct gagcaggagc gtggtgagga agttgcgctg ctggcgcagg
     8281 cgcagggcga tgacgtcggg gttctcgctc ggcccctcct cgccgcagtt ccaggagcgg
     8341 ttgtggctct cgccgtcgtt gccgtcctcg ccgttggcct cgttgcgctt gtcgttgtac
     8401 gacacgaggt cgcgcagggt gaagccgtcg tgtgcggtga cgaagttgat cgacgcgatc
     8461 ggcttgcggc cgctgtgctc gtagaggtcg ctggagccgg tcaggcgcga ggcgaactcc
     8521 ccgagggtgc ggggctcgcc gcgccagaag tcgcgcacgg tgtcgcggta cttgccgttc
     8581 cactccgtcc ac
//
LOCUS       DAOSOC010000105         8512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_8262_length_8512_cov_8.945134, whole genome shotgun sequence.
ACCESSION   DAOSOC010000105 DAOSOC010000000
VERSION     DAOSOC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8262_length_8512_cov_8.945134"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PL014_08710"
     CDS             <1..637
                     /locus_tag="PL014_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT57210.1"
                     /translation="HLVLTTGVVDTLPDVPGLAEHWGTGVIHCPYCHGWEVRDQRVVV
                     LATSPAAMHQAGLFHQLTDALTVVLHDPQALSSDDVARLRALGIDVVEGPVSRVLDEA
                     GTLTGVEVPGGTVRADAVVVASFVEPRSPLLTSLGLDLTDLEMMGRVMARSVAADPVG
                     RTAVPRVWAAGNLTDPMSQVVMAAAAGVRTGAMVNAEMVTEDQARRLELLG"
     gene            complement(650..1363)
                     /locus_tag="PL014_08715"
     CDS             complement(650..1363)
                     /locus_tag="PL014_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57211.1"
                     /translation="MTSQTAASPATRNDPQRIVGVTAIALATSLLVQNVVVGGAGAPE
                     YGAPMADVLAYHAQNRGTVAVAVGLEALNLPLLLGFLAGLHGLVRRNERGASWSLFAL
                     AAGAALSAIWAIYAVTWIGVVLSAGEGGQPSPTLELSWQLHAAGLAMALPALGTTFLG
                     AALATHAASLTPPWQRLLGVVGAALLVVAGTASLAIAEGSPMVFVGAAGLAMWVLWLL
                     ATGVRLVRAHRDPARTSVR"
     gene            complement(1524..1946)
                     /locus_tag="PL014_08720"
     CDS             complement(1524..1946)
                     /locus_tag="PL014_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT57212.1"
                     /translation="MEDLDRRIIELLAQDGRISYTDLGKSLGMSTSAVHQRVRRLEQR
                     GVIKGYTAKVDHQALGRQITAFMSITPLDPAAPDDIPERLRELPQIEECHSVAGDENY
                     ILKVRVDTPAELEELIASVRGVANVSTRTTVVLSTPWE"
     gene            1980..>2893
                     /locus_tag="PL014_08725"
     CDS             1980..>2893
                     /locus_tag="PL014_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HOT57213.1"
                     /translation="MSSAAGGPGKLLLLDSASLYFRAFFGVPDQRRTPDEPPTNALRG
                     FIDMIATLVTAHRPTHLVACWDDDWRPQWRVDLIPTYKTHRLVEGSAVAEESPDDLTP
                     QVPLIREALGALGIARLGSPGFEADDVIGTLAHRHRGVMPVDVVTGDRDLLQLVDDAH
                     GIRVLYTGKGGVREPDIATQTYLQERYSVPTGEAYLEMSVLRGDTSDGLPGVKGIGDK
                     TAAQLIAQYGSLAALRAAIDSGDPAIKGARRLNLEAASDYLDVAPTVVRVARDAALPD
                     VDLTLPDAPADTAALQALADTYGVGNPI"
     assembly_gap    2894..2993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2994..5699)
                     /locus_tag="PL014_08730"
     CDS             complement(<2994..5699)
                     /locus_tag="PL014_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT57214.1"
                     /translation="MSSPAERYAAAAARNRRERSELARFTASLEFPLDDFQVRACEAV
                     ELGKGVLVAAPTGAGKTMVGEFAVHLALATGRKAFYTTPIKALSNQKFHDLVRVHGAA
                     NVGLLTGDSSVNGEAPIVVMTTEVLRNMMYAGSRTLEGLGFVVMDEVHYLADRFRGAV
                     WEEVIIHLPEHVQVVSLSATVSNAEEFGDWLAEVRGNHEVVVSEHRPVPLYQHVLVGT
                     TMFDLFAGNRVNPDLLTAIRSAEERGRWQDEPRRGGGGRGGSRGGGRGPGGGSGGRGF
                     ARGARPGGGASRAEVIEELDRDGLLPAITFIFSRVGCEAAVGQLLAAGTRLVPEREGL
                     RIRRLVEERVDSLATEDLMVLGYFDFVEGLSRGFAAHHAGMLPLFREIVEELFTAGRI
                     RAVFATETLALGINMPARTVVLEKLVKYNGEAHVDITPAEYTQLTGRAGRRGIDIEGH
                     AVVQWARGVDPLAVGGLASARTYPLRSSFRPTYNMAVNLVSQVGREVAREILETSFAQ
                     FQADRAVVGIATKVRRNEEGLHDYDEAMHCHLGDFREYAALRRSLADAEKDGVRARSA
                     SRRAEAALSLEALKIGDVIRIPAGRRAGWAVVVQPARTGRGAPTGPSVVTEDKQFRRL
                     TTVDVPEPVEPMARVSVPAHFNPKSPKSRRDLATSLRIAAPNGLDDAPSRRSRRGGDT
                     EPEHVRMLREQLRAHPCHQCPDREAHARWAERWWRLKRETVGLQRKVEGRTNTVARTF
                     DRICDALEALGYLDEYGTVVTERGERLRRLYTEKDLLAAECLRHDVWKRLDAPSLAAA
                     VSALVHEPRHQEAEVSPRMPNDDVAAALTAMERLWSQIEDLETEHDLPTTTMPDGGMA
                     WMVHRWASGDRLDAVLRGQEMAAGDFVRRCKQLVDLLDQVAKASTD"
     gene            complement(5721..6608)
                     /locus_tag="PL014_08735"
     CDS             complement(5721..6608)
                     /locus_tag="PL014_08735"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOT57215.1"
                     /translation="MARMGLMINPTSGKNTGAQVGQQALSLLRDAGVDVLDLSASDAR
                     SAVDQGRAAIESGAIDRLVVGGGDGMVHLGANLCAGTPVSLGVIAAGTGNDIARELGL
                     PVRDAAASVQRILSGSTRQVDAARHTTATGEQKWFLGVLAAGFDAVVNERANQLTWPK
                     GPMRYNLAILRELPVFRAIPYTLVLDGERIDTEAMLVAVGNGPAYGGGMRVTPDASFD
                     DGLLDVLILRKISTFEFLRVFPRVFKGTHVSHHAVQIRRARTVSLEAEGIVSYADGER
                     FAPLPMSMEVVPGALTVLA"
     gene            6656..8248
                     /locus_tag="PL014_08740"
     CDS             6656..8248
                     /locus_tag="PL014_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOT57216.1"
                     /translation="MNTTFTLLAEHPLLLLAVLLAFGAALGHVRVKGVHLGPAAVLFA
                     ALGVSAWAASADVDLEIPEVIGTFGLVLFTYTVGVVSGTHFFSSLRRGWPTMLVVAGA
                     LSLVGAIAVVVGRILGIDGGTIAGAYAGALTNTPALAAASARAADPAAPTVGYSITYL
                     GGVILMLAVAAWSLRRPGGESRREEIGHETIRVEVTEPITVDALSAAHGRAITVSRLK
                     HAHAANPTMVPADTEVIGHNDLVTVVGPRDLLDAVARQLGHISSHDIVADRADLDYRR
                     ITLSTKSLVGHTIGELDLDARFGAAVTRVRRGDLDLVAHDTFVLAMGDRVRVIAPRDK
                     MAAVSAYLGDSDRGMSDINVGGLALGLATGMALGLVHVPTPGGGFTVGAAAGTLLVGL
                     VFGRLGRVGPVITSMSHGAAQSLSALGMVTFLAYAGVRAGRTITEALASDSGWKVAVL
                     GLVLTASAAVLLVLGVHVLRKLTWLETAGALAGSQTQPAILAYVNDKTGYDTRVGVAY
                     ALVYPVAMIAKIIVAQVLAGLS"
BASE COUNT         1196 a         3072 c         2892 g         1252 t
ORIGIN      
        1 ccacctcgtc ctgaccacgg gagtcgtcga cacgctgccc gacgtccccg ggctggccga
       61 gcactggggc accggcgtca tccactgccc ctactgccac ggctgggagg tgcgcgacca
      121 acgcgtcgtc gtgctcgcga cgtcccccgc agcgatgcac caggccggcc tgttccacca
      181 actgaccgac gccctcacgg tggtgctcca cgacccgcag gcgctgtcgt ccgacgacgt
      241 tgcccgactg cgggcgctgg gcatcgacgt cgtcgagggc ccggtcagca gggtgctcga
      301 cgaggccggc accctcacgg gcgtcgaggt ccccggtggg acggtcaggg cggatgccgt
      361 cgtcgtcgcg tcgttcgtcg agccgcggtc ccccctgctc acctcactgg gactggacct
      421 caccgacctc gagatgatgg gccgcgtcat ggcccgatcc gtggctgcgg accccgtggg
      481 tcgcaccgcc gtgccgcggg tgtgggccgc ggggaacctc accgacccga tgtcccaggt
      541 cgtcatggcc gccgcggccg gcgttcgcac cggggcgatg gtcaacgccg agatggtgac
      601 cgaggaccag gcacgacggc tggagctgct gggctgatgc tcgaggcact cacctgacgc
      661 tggtgcgtgc ggggtccctg tgtgcgcgga ccagccgaac gcccgtcgcg agcagccaca
      721 ggacccacat cgcgaggccg gccgccccga cgaagaccat cggcgagccc tccgcgatgg
      781 ccaggctcgc cgtcccggcg acgacgagca gggcggcccc cacgacaccg agcagccgtt
      841 gccacggggg cgtcagcgag gctgcgtgag tcgccagtgc cgcaccgagg aacgtcgtgc
      901 cgagtgctgg cagcgccatg gccaggcccg cggcatgcag ctgccaggac agctccagcg
      961 tcgggctcgg ctgtccccct tcaccggcgg agaggacgac cccgatccac gtcacggcat
     1021 agatcgccca gatggccgac agcgccgcac ccgcggccag ggcgaacaga gaccacgacg
     1081 cgcccctctc gttgcgccgg accaggccat ggagaccagc caggaacccc agcagcagcg
     1141 gcaggttgag ggcttcgagc cccaccgcca ccgcgacggt gcctcggttc tgcgcgtggt
     1201 aggcgaggac gtccgccatc ggagccccgt actcgggcgc tccggcgcca ccgaccacga
     1261 cgttctggac cagcagcgag gtggccagcg cgatcgccgt cacgccgacg atccgctgag
     1321 ggtcgttgcg tgtcgccggg gacgctgctg tctgtgaggt catcagggtg ggtccttcgg
     1381 ggtgggtcca atgggtgggt cccaccacga tccccgacat cacccgaggt cccatccccc
     1441 tccagaccgt cgctggaccg gccagaagaa cgatgcgcgg gccgttcttt cggctgggtc
     1501 agaccccacg ccaccagctc cagctactcc cagggcgtgg agaggacgac agtcgtgcgc
     1561 gtcgagacgt tggcgacgcc gcgcacgctc gcgatgagtt cctcgagctc ggccggggtg
     1621 tcgacccgca ccttgaggat gtagttctcg tcgccggcca ccgagtggca ctcctcgatc
     1681 tgcggcagct cgcgcaaacg ctcgggaatg tcgtccggag cagccgggtc caggggggtg
     1741 atcgacatga aggcggtgat ctgacgaccg agcgcctggt ggtcgacctt cgccgtatat
     1801 cccttgatca ccccgcgctg ctcgagccgg cgcacccgct ggtgcacggc cgaggtggac
     1861 atgcccaagg acttccccag gtcggtgtag ctgatccgcc cgtcctgggc caggagctcg
     1921 atgatccgcc gatctaggtc ttccacgcgc tcagggtagc cgttctctac gctgcaccca
     1981 tgtcttctgc cgccggtggg cccggcaagc tcctgctgct ggacagcgcc tcgttgtact
     2041 tccgcgcgtt cttcggggtg cccgaccagc gacgcacccc cgacgagccg ccgaccaacg
     2101 ccctgcgcgg attcatcgac atgatcgcca ccctcgtgac ggcccatcgc ccgacccacc
     2161 tcgttgcctg ctgggacgac gactggcggc cgcagtggcg cgtcgacctc atcccgacgt
     2221 acaagacgca tcggctcgtc gagggctcgg ccgtcgcgga ggagtcaccc gacgacctca
     2281 caccgcaggt gccgttgatc cgcgaggcac tgggcgccct cgggatcgcc cgcctcggct
     2341 cgcccgggtt cgaggccgac gacgtcatcg gcacgctggc ccaccgccac cggggagtca
     2401 tgcccgtcga cgtcgtgacc ggtgaccgcg acctgctcca gctcgtcgac gacgcgcacg
     2461 gcatccgggt cctctacacg ggcaagggcg gggtgcgtga gccggacatc gcgacccaga
     2521 cctacctcca ggagcggtac tcggtgccca ccggtgaggc ctacctcgag atgtccgtcc
     2581 tgcgcggcga caccagcgac gggctgccgg gggtcaaggg catcggtgac aagaccgcgg
     2641 ctcagctgat cgcgcagtac ggctccctcg cggcactgcg ggccgcgatc gacagtggtg
     2701 accccgcgat caagggggca cgacgactca acctggaagc ggcatccgac tacctcgacg
     2761 tcgcaccgac ggtggtgcgg gtggccaggg atgccgccct cccggacgtc gacctcacgc
     2821 tccccgacgc gccggcggac accgccgcgc tccaggcgct cgccgacacg tacggcgtcg
     2881 gcaacccgat cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcggtg
     3001 ctggccttgg ccacctggtc gagcaggtcg acgagctgct tgcagcggcg cacgaagtca
     3061 ccggcagcca tctcctgccc tcgcaggacc gcgtcgagcc ggtcccctga ggcccagcgg
     3121 tgcaccatcc acgccatccc gccgtccggc atcgtcgtgg tcggcaggtc gtgctcggtc
     3181 tcgaggtcct cgatctgcga ccagagccgc tccatcgcgg tgagcgccgc agcgacgtcg
     3241 tcgttgggca tcctcggcga gacctcggcc tcctggtggc gcggttcgtg cacgagagcc
     3301 gagaccgccg ccgcgagcga cggggcgtcg agccgcttcc agacgtcgtg ccgcaggcac
     3361 tccgcagcca gcaggtcctt ctcggtgtag agccggcgca gccgctcacc ccgttcggtg
     3421 accacggtgc cgtactcgtc gaggtacccc agcgcctcga gcgcgtcgca gatgcggtcg
     3481 aaggtgcggg ccacggtgtt ggtgcggccc tcgaccttgc gctgcagccc caccgtctcg
     3541 cgcttgaggc gccaccagcg ttcggcccag cgggcgtggg cctcgcggtc cgggcactgg
     3601 tggcaggggt gggcgcgcag ctgctcacgc agcatccgca cgtgttcggg ctcggtgtcg
     3661 ccaccacgac gtgaccggcg cgacggggcg tcgtcgagtc cgttgggcgc agcgatccgc
     3721 agcgacgtcg cgaggtcgcg gcgcgacttc ggtgacttgg ggttgaagtg ggcgggcacg
     3781 ctcacccggg ccatcggctc gacgggttcg gggacgtcga cggtggtcag ccggcggaac
     3841 tgcttgtcct cggtcaccac gctcggcccg gtgggagccc cccggccggt tcgggcaggc
     3901 tgcaccacga cggcccagcc tgcccgacgc cccgccggga tgcggatgac gtcgccgatc
     3961 ttcagggcct ccagcgacag ggcggcctcg gcgcgacggg acgccgatcg cgcgcgcacc
     4021 ccgtccttct ccgcgtcggc gaggctgcgg cgcagggccg cgtactcccg gaagtcgccg
     4081 aggtggcagt gcatcgcctc gtcgtagtcg tgcagcccct cctcgttgcg gcgcaccttc
     4141 gtggcgatgc ccaccacggc ccggtcggcc tggaactgcg cgaacgaggt ctcgaggatc
     4201 tcgcgggcca cctcacgacc cacctgggac acgaggttga cggccatgtt gtaggtcggg
     4261 cgaaagctcg agcgcagtgg gtaggtgcgg gccgaggcca gaccgccgac cgccaacggg
     4321 tcgactcccc gggcccactg caccaccgcg tggccctcga tgtcgatgcc acggcgcccg
     4381 gcacgcccgg tgagctgggt gtactcggcg ggggtgatgt cgacgtgggc ctcgccgttg
     4441 tacttgacga gcttctccag caccaccgtg cgggccggca tgttgatgcc cagggcaagg
     4501 gtctcggtgg cgaacaccgc acggatgcgc ccggccgtga acagctcctc gacgatctcg
     4561 cggaacagcg gcagcatgcc ggcgtggtgg gcggcgaaac ccctcgacag gccctcgacg
     4621 aagtcgaagt agccgaggac catgaggtcc tccgtggcca gggagtccac gcgctcctcg
     4681 acgaggcgcc ggatccgcag cccctcgcgc tcgggcacca gccgggttcc cgcggcgagc
     4741 agctggccga cggcggcctc gcacccgacc cggctgaaga tgaaggtgat ggccgggagc
     4801 agcccgtcgc gatcgagctc ctcgatgacc tcggcacggc tcgcaccccc gccgggtcgg
     4861 gcgccgcggg caaatcctcg accgcccgat cctccaccgg ggccacgacc gcccccgcgc
     4921 gacccgccgc gacctccccc accccggcgg ggctcgtcct gccaccgtcc gcgttcctcc
     4981 gcgctgcgga tggcggtcag caggtcgggg ttgacccggt tgccggcgaa caggtcgaac
     5041 atcgtcgtgc cgacgaggac gtgctggtac agcggcaccg gacggtgctc ggacacgacg
     5101 acctcgtggt tgccccgcac ctcggccagc cagtcgccga actcctcggc gttgctcacc
     5161 gttgccgaca gcgacaccac ctgcacgtgc tcgggcaggt ggatgatcac ctcctcccac
     5221 acggccccgc ggaaccggtc ggcgaggtag tggacctcgt ccatgacgac gaagccgagc
     5281 ccctcgaggg tgcgcgaccc ggcatacatc atgttgcgca gcacctcggt cgtcatgacg
     5341 acgatcgggg cctcgccgtt gaccgacgag tcccccgtca gcagaccgac gttcgcggcc
     5401 ccgtgcaccc gtacgaggtc gtggaacttc tggttcgaca gggccttgat gggggtggtg
     5461 tagaacgcct tgcgaccggt cgcgagcgcc aggtgcacgg cgaactcgcc gaccatcgtc
     5521 ttgccggccc ccgtgggcgc tgcgacgagc acccccttgc cgagctcgac cgcctcgcag
     5581 gcccgcacct ggaagtcgtc gagggggaat tccagggatg ccgtgaagcg cgcgagttcg
     5641 ctgcgttccc ggcgattgcg ggccgctgcc gcggcgtacc tctcggcggg gctggacatg
     5701 cgctcaggct acgtcagcga tcacgcgagg acggtgagcg cgccgggcac gacctccatg
     5761 ctcatcggca gcggggcgaa gcgctcgccg tcggcgtagg agacgatgcc ctcggcctcg
     5821 agcgacaccg tgcgcgccct gcggatctgc acggcgtgat ggctcacgtg ggtgcccttg
     5881 aacaccctcg ggaacacccg gaggaactcg aaggtcgaga tcttgcgcag gatgagcacg
     5941 tcgagcagcc cgtcgtcgaa ggacgcgtcc ggggtgaccc gcatgccgcc gccgtaggcg
     6001 ggtccgttgc cgacggcgac gagcatcgcc tcggtgtcga tgcgctcacc gtccagcacg
     6061 agcgtgtacg ggatcgcacg gaagaccggg agctcacgca ggatcgcgag gttgtagcgc
     6121 atcgggccct tgggccaggt cagctggttg gcgcgctcgt tgaccaccgc gtcgaagccg
     6181 gcggcgagca ccccgaggaa ccacttctgc tcgccggtgg ccgtggtgtg ccgcgcggcg
     6241 tcgacctgac gggtgctccc cgacaggatg cgctgcaccg aggcggccgc gtcgcgcacc
     6301 ggcaagccca gttcgcgggc gatgtcgttg ccggtgccgg ccgcgatcac cccgagggac
     6361 accggcgtgc ccgcacacag gttcgcgccc aggtgcacca tgccgtcccc gccacccacg
     6421 acgagccgat cgatcgcgcc cgactcgatc gcggccctgc cctggtccac tgcactgcgg
     6481 gcatcggacg cgctgaggtc gaggacgtcc acaccggcgt cccgcagcag ggacagggcc
     6541 tgctgaccga cctgcgcccc ggtgttcttg cccgaggtcg ggttgatcat caaacccatg
     6601 cgtgccatgt atcgaacggt agtggcagcg atcctcgtgg tgggacgatc caggggtgaa
     6661 cacgacattc accctgttgg ccgaacaccc cctcctcctc ctcgcggtgt tgctggcgtt
     6721 cggcgccgca ctggggcacg tgcgcgtcaa gggggtgcac ctcgggccgg ctgccgtgct
     6781 gttcgccgcc ctcggggtct ccgcgtgggc ggcctcggct gacgtcgacc tcgagatccc
     6841 cgaggtcatc ggcacgttcg gcctcgtgct cttcacgtac acggtgggtg tcgtctcggg
     6901 cacccacttc ttctcgtcgt tgcgccgggg ctggccgacg atgctcgtcg tcgcgggcgc
     6961 gctctcgctc gtcggcgcca tcgcggtggt cgtcggccgc atcctcggca tcgatggcgg
     7021 cacgatcgcc ggtgcgtatg ccggtgcgct caccaacacc cccgcgctcg ccgcggcatc
     7081 cgcccgcgca gccgacccgg cggccccgac ggtcgggtac tccatcacct acctcggggg
     7141 cgtcatcctc atgctcgccg tcgccgcatg gtcgctccgt cgacccggtg gcgagtcgcg
     7201 gcgcgaggag atcgggcacg agacgatccg cgtcgaggtg accgaaccca tcaccgtcga
     7261 cgccctctca gcagcccacg gccgggccat caccgtgtcg cggctcaagc acgcgcacgc
     7321 cgccaacccg acgatggtgc ccgcggacac cgaggtgatc gggcacaacg acctcgtcac
     7381 cgtcgtcggc ccgcgtgacc tgttggatgc cgtggctcgc cagctcggcc acatctcgtc
     7441 ccacgacatc gtcgccgacc gggcagacct ggactaccgg cgcatcacgt tgtcgaccaa
     7501 gtccctggtc ggccacacca tcggcgagct cgacctcgac gcgcggttcg gcgcggccgt
     7561 gacgagggtg cgtcgcggcg acctcgacct cgtcgcccac gacaccttcg tcctcgccat
     7621 gggtgaccgg gtgcgggtca tcgccccccg ggacaagatg gctgctgtca gcgcctacct
     7681 cggcgacagc gaccgcggca tgtcggacat caacgtcggg gggctggccc tcgggctcgc
     7741 gacgggcatg gcgctcgggc tcgtccacgt gcccactccc ggtggtggct tcaccgtcgg
     7801 tgccgctgcc ggcaccctgc tcgtcggcct cgtgttcggt cgactcggtc gggtcgggcc
     7861 ggtcatcacc tccatgtccc acggcgcggc gcagtcgctc tcggccctcg ggatggtcac
     7921 gttcctcgcg tatgccgggg tgcgcgccgg ccggaccatc accgaggcgc tggcctcgga
     7981 ctcgggctgg aaggtggccg tgctgggact ggtcctcacg gcatccgccg ccgttctcct
     8041 cgtcctcggc gtccacgtcc tgcgcaagct cacctggctg gagaccgccg gggcgctggc
     8101 cggctcgcag acccaaccgg cgatcctcgc ctacgtcaac gacaagaccg gctacgacac
     8161 ccgcgtcggg gtggcctacg cgctggtgta cccggtggcg atgatcgcga agatcatcgt
     8221 ggcgcaggtg ctcgccgggc tgagctgacc tgcggttcgt cggcggcgac gtccgcgatg
     8281 gacccacgca gaacttcgga tggcacgcag caactgctgc gtgccatccg aagttctgcg
     8341 ttctcagagc gcggaggcct cgttgtcgtc cacgttgagc cagtcagggc gctgcttggc
     8401 cttgcgtcgg tccaagaacc aacagatgaa ccatgccatg aagtacagag tcgtcaacgg
     8461 ccccgcgaag acgaacatgg tgtacggatc aggcgtcggg gtggccacgg cg
//
LOCUS       DAOSOC010000106         8405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_8410_length_8405_cov_8.500599, whole genome shotgun sequence.
ACCESSION   DAOSOC010000106 DAOSOC010000000
VERSION     DAOSOC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8410_length_8405_cov_8.500599"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PL014_08745"
     CDS             complement(<1..675)
                     /locus_tag="PL014_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143067.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HOT57217.1"
                     /translation="MNSEESEGLCPVIHTANHPTEGAGNRTWWPDQLNLKILRKHAPA
                     ANPMGEDFDYAEAFASLDLDALRADLTALMTDSQDWWPADYGHYGPLFIRMAWHGAGT
                     YRISDGRGGAGAGMQRFAPLNSWPDNANLDKARALLWPIKKKYGKQISWADLMVLTGN
                     VALESMGFKTFGFGGGRADVWEPDEDVYWGPEDTWLGDERYTGDRELENPLAAVQMGL
                     IYVNPEG"
     gene            complement(773..1210)
                     /locus_tag="PL014_08750"
     CDS             complement(773..1210)
                     /locus_tag="PL014_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012880053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOT57218.1"
                     /translation="MPSSTDLEQLLREHHLRVTRPRLAVLGAVHSSPHASTDAVIDAV
                     RGELGVVSHQAVYDVLRALTTAGLLRRIQPAGSVARYEARVGDNHHHVVCRTCGAIAD
                     VDCAVSATPCLTASDDQGFVIDEAEVTYWGTCATCAQVAHAAR"
     gene            1287..1607
                     /locus_tag="PL014_08755"
     CDS             1287..1607
                     /locus_tag="PL014_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT57219.1"
                     /translation="MTKTIDITTLRARHADGAVVLDVREPAEYVGGHVPGAILAPMSR
                     ITASLGGVPNDQTVHVICQSGNRSRSMADLLTALGYDAVSVDGGTAGWIAAGLPVVRG
                     PQAA"
     gene            1637..2308
                     /locus_tag="PL014_08760"
     CDS             1637..2308
                     /locus_tag="PL014_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT57220.1"
                     /translation="MPGSDWTRVLVVVAHPDDESFALGALIDRAVRLGAEVSVLCLTR
                     GEASTLGAGTGDLAAQRAAELSDAARALGCARTTLLAHPDGALADLDVRVLEADVEAE
                     VRATRPDGILVFDPLGGVTGHPDHRAASEAALAVAARHGIPVLGWALPQEVSRVLNDN
                     HGAAFAGHPLHDLTAIPVDRRSQSAAVHCHASQAVPGSVLWQRLELLGDVEYVRVLAP
                     AVSPA"
     gene            complement(2316..2516)
                     /locus_tag="PL014_08765"
     CDS             complement(2316..2516)
                     /locus_tag="PL014_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57221.1"
                     /translation="MRYQFIVSGSVSDDVMAALPELRATPYPTGGTVLFGPVRDVADI
                     STLLARIADLGLSVVEMRPLPD"
     gene            complement(2632..5343)
                     /locus_tag="PL014_08770"
     CDS             complement(2632..5343)
                     /locus_tag="PL014_08770"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT57222.1"
                     /translation="MTAVRETPSAATPRLCVPRRIDGALERPRLLLTRSQEAWTLAGT
                     DLLLACAPAGYGKTTVLIDVAEQCRAHGLPIAWVTCGRDVDGKSFWNAVLDALVVAVG
                     HPAEALTRLHAPAGAPDPGFVAALLAAIREVAPGLVLVLDDVHEVVDGDALAGIRLLV
                     EWAGPEVRIAFGCRFEPPVGLHLARVAGRLHELRANDLSFTSDEADSFWARFGLDLDD
                     DVRGAMLRLTEGWPAGLRLAAMSLEEGGDPGTFVEQWSGDDRPVADYLAGEVLSRLPE
                     EFVTFLQRTSIVDEVDVDLAAHLSGRFDAAALLDDLAQRNALVIRLDRHGRWFRYHAL
                     LRTHLMAALRRRDPASVAALHGTAARWFADRDQPVAALDHACAAGDPALVDAILRSHG
                     VGLLMAGATQGVRRALGRPALGGPQTPTVLIHRALLAVDDGDLAQADEALAALAGQPD
                     DGADRRLTSLRRAAALHRARLAADLDQAQASELFGAHGAAPLPPDLDPDVRLLVLGDR
                     GALRLVEGDHAGARDDLRRAIDLAQAAGLPSIQLFCKNLIAGAHLAQSDFVGARLAAE
                     RAITFASERGWARSPGLAYSYALAGWTSFQALRPADAAFWADTALDVIEGGHTTIDVE
                     VEGGARAGAAIIAFDRHPDRRAALDRLVRVTAWRAERGGSSAFTAIAAPHELRMCLSL
                     GEWQRAEQSVARAHQSLGASGDVLVLQAHLAAARGRPTDARRFLVPVLRGREVPLRET
                     AVTAAWLLEALLATRADRGPAATEALLTALALAEPTGACRPFFDAGLEVHTLLTGLHG
                     RAGHLEPFLDRVVTGITEVLEWQRAEGTNHDGGRGASTTGSGVWLTERELVVLRDLPS
                     MMTLGEIGEAQGISLNTVKTHVRSIYAKLGAGTRREAISAARDLGLL"
     gene            complement(5452..6840)
                     /locus_tag="PL014_08775"
     CDS             complement(5452..6840)
                     /locus_tag="PL014_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HOT57223.1"
                     /translation="MADLSPAERRKHGKSARTHAPLEGQAQVGAAADRDAVGLLLGQA
                     DSRVPELVPIRHGRMLASPFTFFRGAALPMAADLAMTPDSGLRVQLCGDAHLSNFGIF
                     ASPDRHLVFDLNDFDETLPGPFEWDVKRFAASLAVAGRDLGLSAKDRHSLVRAGVATY
                     RTSMAEFASKSLLEVWYSRLDVEEALEKVAPQVGAEGVGRTRKALAKARKRDSLQVLG
                     KLTTVEGGHRRIISQPPLVVPIEELLDDVGADQLYRFLDEILEDYRRTLTADRRHLLN
                     QFRVVGAARKVVGVGSVGTRAWTVLLEADGGKLPLFLQIKEAQRSVLADYCGDGIHDH
                     EGERVVSGQHLMQAASDIFLGWQRITGVDGVVRDFYVRQLRDWKFSLPLESMTLAGLT
                     TYAQLCGWTLARAHARTGDRFAIAGYLGKSDRFDRALADFAEQYADITEQDHAALAAA
                     VEEGRAQAIHDV"
     gene            complement(6882..7190)
                     /locus_tag="PL014_08780"
     CDS             complement(6882..7190)
                     /locus_tag="PL014_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOT57224.1"
                     /translation="MARTAVIAGTASSVSGRVQRRQAEKYADRDAAIAAQRSQAYQDQ
                     MAAAAPAPPAAAPAPAPAPAAPAMTSDLITQLKSLAELRDQGILTEDEFAAQKAKLLG
                     "
     gene            complement(7216..7665)
                     /locus_tag="PL014_08785"
     CDS             complement(7216..7665)
                     /locus_tag="PL014_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HOT57225.1"
                     /translation="MTTTDTERAHGPIDFLLLEFPSADRQSEAADAMFELVDNGTVRL
                     LDLVVVRKFADGGVEVLDLDTLAGEVSFTRFSGARSGLLGPEDIAQASEAIEHGSLAA
                     LIVFENSWAAPFVAAVRRGGGDVIASMRIPAADVMDALDALESTESE"
     gene            7816..>8405
                     /locus_tag="PL014_08790"
     CDS             7816..>8405
                     /locus_tag="PL014_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57226.1"
                     /translation="MLPGLGLRSRILVLTMPLVLLGHAFGAGIVYLSLGQVLEAAARR
                     SASSEVAEVRADLALHESGEDVAYHVAAGPDRVVQIVEPSGTLVATSMPALGTPIHDP
                     AESTEEVRVVIDDDVPGLGGRTYAIGMSDATTIGGQRRVVLVAVPTDLGASALGPTVT
                     FGVLGLIGIILILATVTTIAVGQALRPVERMRRQIDG"
BASE COUNT         1161 a         2885 c         3106 g         1253 t
ORIGIN      
        1 gccttcgggg ttgacgtaga tcagacccat ctgcacggcg gccagcgggt tctcgagctc
       61 gcggtcaccg gtgtagcgct cgtcgccgag ccaggtgtcc tccgggcccc agtagacgtc
      121 ctcgtcgggc tcccacacgt cggcgcgacc gccgccgaag ccgaaggtct tgaagcccat
      181 cgactccagt gcgacgttgc cggtgagcac catcaggtcg gcccaggaga tctgcttgcc
      241 gtacttcttc ttgatcggcc agagcagggc gcgggccttg tcgaggttgg cgttgtcggg
      301 ccagctgttc agcggcgcga accgctgcat ccccgctccg gcgccaccgc gaccgtcgct
      361 gatccggtag gtgccggcgc cgtgccaggc catccggatg aagagcgggc cgtagtgccc
      421 gtagtcggcc ggccaccagt cctgggagtc ggtcatgagc gcggtgaggt cggcgcgcag
      481 ggcgtcgagg tcgaggctcg cgaacgcctc ggcgtagtcg aagtcctcac ccatcgggtt
      541 ggctgcgggc gcgtgcttgc gcaggatctt caggttgagc tggtcgggcc accacgtgcg
      601 gttgccagca ccctcagtgg ggtggttggc ggtgtggatg acgggacaca gtccctcgct
      661 ctcctccgag ttcatgtctc cgacgacggc gtcgtggtgc tgctcgctca tgctcttcct
      721 tccaatgtgc tggtggggca gggtggtggt caggctgcgt gggtggtcgg tttcatcgtg
      781 ccgcgtgtgc gacctgtgcg caggtcgcac aggttcccca gtacgtgact tcggcctcgt
      841 cgatcacgaa gccctggtcg tcggatgccg tgaggcacgg cgtcgcggac accgcgcagt
      901 ccacgtcggc gatcgccccg caggtgcgac agacgacgtg gtggtggttg tccccgaccc
      961 gggcctcgta ccgggcaacg gagccagcgg gttggatgcg gcgcagcagg cccgcggtgg
     1021 tgagggcgcg cagcacgtcg tagacggcct ggtgggacac cacgccgagc tcgccccgga
     1081 cggcgtcgat gacggcatcc gtgctcgcgt gcggcgagga gtggactgcg ccgaggacgg
     1141 ccagccgcgg ccgggtgacc cgaaggtggt gctcgcgcag cagctgctcg agatcggtgg
     1201 aggacggcac aacgacgagt ctgccgctct tcttgagtaa gtcaagagtt tttcgacata
     1261 gatacccctg ggggtatgat ggtcccatga cgaagaccat tgacatcacc accctccggg
     1321 cccggcacgc cgacggtgcc gttgtcctcg acgtccgtga gcccgctgag tacgtcgggg
     1381 gtcacgtccc cggcgcgatc ctggctccga tgagccgcat cacggcctcg ctcggcgggg
     1441 tgcccaacga ccagaccgtg cacgtcatct gccagagcgg caaccgcagc cgctccatgg
     1501 ccgacctgct cacggccctg ggctacgacg cggtctccgt cgacggtggc accgctggct
     1561 ggatcgcggc cgggctccct gtcgtccggg gtccgcaggc agcctgatgc cggacgtgcg
     1621 ggcagaggaa cccgtcgtgc ccggatctga ctggacgcgg gtgctcgtcg tcgtcgctca
     1681 ccccgacgac gagtcgttcg cactcggcgc gctcatcgac cgcgccgtcc gtctcggcgc
     1741 cgaggtgagc gtgctgtgcc tgacgcgcgg tgaggcctcg accctcgggg cagggacggg
     1801 cgacctggca gcccagcggg cagccgagct gtcggatgcc gcacgcgcgc tggggtgtgc
     1861 gcgcacgacc ctgctcgcgc acccggacgg cgccctggcc gacctcgacg tccgggtcct
     1921 cgaggccgac gtggaggccg aggtccgcgc gacgcgaccg gacggcatcc tcgtgttcga
     1981 ccctctcggg ggtgtcaccg ggcaccccga ccaccgcgct gcgtccgagg ctgcgctcgc
     2041 cgtggccgct cgacacggca tccccgtgct gggctgggcc ctgccgcagg aggtgagccg
     2101 ggtgctgaac gacaaccacg gggcggcctt cgcgggtcat ccgctccacg acctcaccgc
     2161 catccccgtt gaccgtcgca gccagtccgc ggcggtgcac tgccacgcca gccaggcggt
     2221 ccccggcagc gttctctggc agcgactgga gctcctcggc gacgtcgagt acgtgcgcgt
     2281 gctggctcct gcggtgagcc cggcctgatt ccgcgtcagt cggggagggg gcgcatctcg
     2341 acgacggaca gcccgaggtc ggctatccgg gccaggagcg tggagatgtc ggccacgtca
     2401 cgcaccggcc cgaacagcac cgtgccgccg gtggggtagg gggtggctcg cagttcggga
     2461 agcgcagcca tgacgtcatc ggacaccgat ccgctgacga tgaactggta tcgcatctgt
     2521 gccttctccc gcgccatggt ggcctcgctc cccgccagcg tgacagcagc cgcccggccc
     2581 ggcctcacgc gtagcgggtg agcctgcgcg ggtcgaggcg ttgggcgggg gtcagagcag
     2641 gccgaggtcg cgggcagcgc tgatggcctc gcgccgcgtg cccgctccga gcttcgcgta
     2701 gatcgagcgc acgtgggtct tcacggtgtt gaggctgatg ccctgtgcct cgccgatctc
     2761 gccgagggtc atcatcgagg gcaggtcccg cagcacgacg agctctcgct cggtgagcca
     2821 cacaccggac ccggtcgtcg acgcccctct gccgccgtcg tggttcgtcc cctccgcgcg
     2881 ctgccactcg agcacctcgg tgatgccggt gacgacgcgg tcgaggaacg gctcgaggtg
     2941 gccggcgcga ccgtgcaacc cggtgagcag cgtgtggacc tcgaggccgg cgtcgaagaa
     3001 cggcctgcag gcgcccgtgg gttcagcgag cgccaacgcg gtgagcaggg cctcggtggc
     3061 ggccggccct cgatcggctc gggtcgcgag cagggcttcg agcagccacg ccgcagtgac
     3121 ggcagtctcc cgcagcggca cctcccggcc gcgcagcacc ggcacgagga agcggcgggc
     3181 gtcggtgggc cgtccgcgcg ccgcagccag gtgcgcctgc aggacgagca catcgccgct
     3241 ggcgcccagg ctctggtgag ctcgcgccac ggactgctcc gcgcgctgcc actctccgag
     3301 gctgaggcac atccgcagtt cgtggggtgc cgcgatcgcc gtgaaggccg aagacccgcc
     3361 gcgttcggca cgccacgccg tgacccggac caaccgatcc agcgcagcgc gacggtcagg
     3421 gtgcctgtcg aaggcgatga tggcggcgcc ggcgcgggcg ccaccctcga cctcgacgtc
     3481 gatcgtggtg tgtccgccct cgatgacatc gagggcggtg tcggcccaga aggcggcatc
     3541 tgcgggccgc agtgcctgga acgaggtcca ccccgccagg gcgtaggagt acgcgaggcc
     3601 cggggagcgt gcccagcccc gctcgctcgc gaaggtgatc gcgcgctcgg ccgcgagccg
     3661 agcgcccacg aagtcgctct gggcgaggtg ggctcccgcg atgaggttct tgcagaagag
     3721 ctggatcgag ggcagcccgg cagcctgggc gaggtcgatc gcacgtcgca ggtcatcccg
     3781 ggcgcccgca tggtcaccct cgaccagacg cagggccccc cggtcgccga ggacgagcag
     3841 cctgacgtcg gggtcgaggt ccggcgggag cggtgctgct ccgtgcgcgc cgaagagctc
     3901 gcttgcctgg gcctggtcga ggtcagcggc cagacgggcc cggtgcaggg ctgctgccct
     3961 tcgcagcgac gtcagccgcc gatcggcgcc gtcatcgggc tgacccgcga gcgcagccag
     4021 cgcctcgtcg gcctgggcga ggtcaccgtc gtcgaccgcg agcagggccc ggtggatcag
     4081 cacggtcggt gtctgcgggc cgcccagcgc tggccggccg agggcgcgac gcacgccttg
     4141 ggtcgcccct gccatgagca ggccgactcc gtgcgagcgc aggatcgcgt cgacgagggc
     4201 tgggtcgccc gcggcgcagg cgtggtcgag tgccgccacg ggctggtcgc ggtccgcgaa
     4261 ccaccgggcg gccgtgccgt gcagcgcagc gaccgaggcc gggtcccggc gccggagcgc
     4321 ggccatgagg tgggtgcgca gcagcgcgtg gtagcggaac caccgtccat ggcggtccag
     4381 ccggatgacg agggcgttgc gctgcgcgag gtcgtcgagc agggcagccg cgtcgaaccg
     4441 tccgctcaga tgggccgcga ggtcgacgtc cacctcgtcg acgatgctcg tgcgctggag
     4501 gaaggtgacg aactcctccg gcagccggga gagcacctca ccggccaggt agtcggccac
     4561 cggtcggtcg tccccgctcc actgctcgac gaaggtcccc gggtcgccac cctcctcgag
     4621 cgacatggcc gccagccgca gcccggccgg ccagccctcc gtgagtctca gcatcgcgcc
     4681 acgcacgtcg tcatcgaggt cgaggccgaa gcgtgcccag aagctgtccg cctcgtcact
     4741 ggtgaacgac aggtcgttcg cccgcagctc gtgcagacgc ccagccaccc gcgcgaggtg
     4801 aagcccgacg ggtggctcga aacggcatcc gaacgcgatg cgaacctccg ggccggccca
     4861 ctcgacgaga agccggatgc cggccaacgc gtcgccgtcg acgacctcgt ggacgtcgtc
     4921 gagcaccagc accagcccgg gagccacctc gcggatcgct gcgagcaagg cggcaacgaa
     4981 ccccgggtcg ggggctcctg cgggtgcgtg gaggcgggtc agcgcctccg ctgggtggcc
     5041 aacggccacg accagggcgt cgaggactgc gttccagaag gacttcccgt cgacgtcgcg
     5101 cccgcaggtg acccacgcga tcggcaggcc gtgggcccgg cactgttcgg cgacgtcgat
     5161 gagcacggtg gtcttgccgt agcccgccgg agcgcacgcg agcaacaggt cggtgccggc
     5221 gagggtccag gcttcctgag atcgggtgag caacaggcga ggtcgttcga gcgccccatc
     5281 gatccggcgc ggcacgcaca gccggggagt cgcagcggag ggcgtctccc gcacagcagt
     5341 cacgcgtcta cggtagatcc ggttcggcca tcgtgggtag taacgcaaaa cctcgtgacc
     5401 acacgcaccc agcggcgaca gccgtcgccg ggcgtgcgga tgccgggtgg ttcagacgtc
     5461 gtggatggct tgggcgcggc cctcctcgac agcggcggcg agcgccgcgt ggtcctgttc
     5521 ggtgatgtcg gcgtactgct ccgcgaagtc ggccagcgct cggtcgaaac ggtcggactt
     5581 gcccaggtag ccggcgatcg cgaaccggtc tcccgtgcgg gcgtgggcgc gggccagagt
     5641 ccagccacac agctgggcgt aggtggtcag gcccgcgagg gtcatgctct ccagggggag
     5701 ggagaacttc cagtcccgca gctgacggac gtagaagtcg cgaaccacgc cgtcgacgcc
     5761 cgtgatgcgc tgccacccga ggaagatgtc gctggccgcc tgcatgaggt gttggccgga
     5821 gacgacccgt tcaccctcgt ggtcgtggat gccgtcgccg cagtagtcgg cgagcaccga
     5881 gcgctgggcc tccttgatct ggaggaagag ggggagcttg ccgccgtcgg cctcgagcag
     5941 cacggtccac gcgcgggtgc cgacgctgcc gacgcccacg accttgcgag cggccccgac
     6001 cacgcggaac tggttgagca ggtggcgccg atcggcggtg agggtgcgcc ggtagtcctc
     6061 caggatctcg tcgaggaagc ggtagagctg gtccgccccg acgtcgtcga gcagctcctc
     6121 gatcgggacg acgaggggtg gctggctgat gatccggcgg tggccgccct cgaccgtggt
     6181 cagcttgccg agcacctgga ggctgtcgcg cttgcgggcc ttggccaggg ccttgcgggt
     6241 gcggccgaca ccctcggcgc cgacctgggg ggcgaccttc tccagggcct cctcgacgtc
     6301 gagccgtgag taccagacct cgagcaggct cttcgacgcg aactcggcca tgctcgtgcg
     6361 gtaggtcgcg actcccgccc gcacgaggga gtgccgatcc ttcgcgctga ggcccaggtc
     6421 gcggccggcg accgcgaggc tggcggcaaa gcgcttgacg tcccactcga acggccccgg
     6481 aagggtctcg tcgaagtcgt tgaggtcgaa gacgaggtgc cggtccgggg acgcgaagat
     6541 gccgaagttg gacaggtgtg cgtcgccgca gagctgcacc cgcaggcccg agtcaggtgt
     6601 catcgccagg tcggcggcca tcggtagggc agcaccgcgg aagaacgtga acggcgatgc
     6661 cagcatccgc ccgtgccgga tggggaccag ttcggggacc cggctgtcgg cctgcccgag
     6721 gagcaggccg acagcgtcgc ggtcggccgc agcgccgacc tgcgcctggc cctcgagcgg
     6781 ggcgtgggtg cgggccgact ttccgtgctt gcgtcgctca gcgggactga ggtcggccac
     6841 gtcgaacacc atctctcgtc gtcatccggc gtgcgggccg gtcagccgag cagcttggcc
     6901 ttctgggcgg cgaactcgtc ctcggtgagg atgccctggt cgcgaagctc ggccagggac
     6961 ttcagctggg tgatgaggtc gctcgtcatc gccggtgcgg cgggtgcggg cgcgggggcc
     7021 ggtgcagcag cgggcggtgc gggggccgct gccgccatct ggtcctggta ggcctgcgag
     7081 cgctgcgcgg cgatggccgc gtcgcggtcg gcgtacttct cggcctggcg gcgctgcacg
     7141 cggccgctca ccgatgacgc ggttccggcg atgacggccg tacgggccac gcctcgaaga
     7201 agtcctggca ttgtcctact cgctttctgt cgactcgagg gcgtcgagtg cgtccatgac
     7261 gtctgctgcg gggatgcgca tgctggcgat gacgtcgccg ccaccgcgtc ggaccgccgc
     7321 gacgaacggg gcggcccagc tgttctcgaa gacgatgagc gcggccaggg agccgtgctc
     7381 gatcgcctcg ctggcctggg cgatgtcctc ggggccgagc agcccggacc gcgcgccgga
     7441 gaacctcgtg aacgagacct cgccagcgag ggtgtcgagg tcgaggacct ccacaccgcc
     7501 atcggcgaac ttgcggacga ccacgaggtc gagcagccgc accgtcccgt tgtcgacgag
     7561 ctcgaacatc gcgtcggctg cctcgctctg acggtctgcg ctggggaact cgagaagcag
     7621 gaagtcgatc gggccgtgag cccgctcggt gtcggtcgtg gtcatgccgg tccctctcga
     7681 cggtctgggg gtgtctgctc catcaagcca gtctccggca ctgcgcgcct cacccacccg
     7741 gggtgagcgt gcagaccgtg ccgcacggtg ccgcacgggc gatgccgcgc cggacggcat
     7801 ccgtaggatc ctcgtatgct tcccgggctc ggactgcgca gccgcatcct cgtgctgacg
     7861 atgcccctgg tgctgttggg ccacgccttc ggtgcgggga tcgtgtacct gtcgctgggc
     7921 caggtcctgg aggcagctgc ccgacggagc gcctcctcgg aggtcgcgga ggttcgcgct
     7981 gacctcgcgc tgcacgagag cggtgaagac gtcgcctacc acgtggccgc cggccctgat
     8041 cgggtcgtgc agatcgtcga gccttcggga acgctggtcg cgaccagcat gccggcgctc
     8101 gggacgccga tccatgatcc ggctgaatcg acggaggagg tccgggtcgt catcgacgac
     8161 gacgtcccgg ggctgggtgg tcgcacgtac gcgatcggga tgtccgatgc gacgacgatt
     8221 ggtggccaac gccgcgtcgt cctcgtcgcc gttcccaccg acctgggtgc ctccgccctc
     8281 ggccccacgg tgaccttcgg ggtgctcggg ctcatcggca tcatcctcat cctcgcgacg
     8341 gtgacgacga tcgcggtggg gcaggcgctg cgcccggtgg aacggatgcg ccgccagatc
     8401 gacgg
//
LOCUS       DAOSOC010000107         8361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_8458_length_8361_cov_9.986997, whole genome shotgun sequence.
ACCESSION   DAOSOC010000107 DAOSOC010000000
VERSION     DAOSOC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8458_length_8361_cov_9.986997"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PL014_08795"
     CDS             complement(<1..749)
                     /locus_tag="PL014_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT57227.1"
                     /translation="MTSLITEGSGLAPVDASGASAFVWLLIALPLLGAAVLLLGGRRT
                     DKFGPLLATAMSWGSFLVGVAIILHLIGLPTEERAQHLTLWNWVPAGSFQLDLGLLAD
                     PLSLTFVMLITFVGSLIHVYSIGYMEHDPDKRRFFAYLNLFIASMLLLVLADSYLLLF
                     VGWEGVGLASYLLIGFWNWNPAYASAANKAFFVNRVGDLGLSIAIMVMFVTFGGVDYA
                     TVNEGVATANQGAITAIGLLLLVGACGKSAQ"
     gene            complement(759..1058)
                     /gene="nuoK"
                     /locus_tag="PL014_08800"
     CDS             complement(759..1058)
                     /gene="nuoK"
                     /locus_tag="PL014_08800"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOT57228.1"
                     /translation="MNPVNYIYLATILFVIGAAVVLTRRNAIIVFMGVELMLNSANLA
                     FVTFARMNGNLDGQVIALFVMVVAAAEVVVGLAIIMAIFRSRRSASVDDANLLKL"
     gene            complement(1055..1825)
                     /locus_tag="PL014_08805"
     CDS             complement(1055..1825)
                     /locus_tag="PL014_08805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOT57229.1"
                     /translation="MTGTGEQVLFWVLGPLAVLGSLGLVFAKKAVHAALGMALTMIIL
                     GIFYIAQGADFLGVIQIFVYTGAVMMLFLFVIMLVGVDSSDSLVETLPGQRWVTAVLC
                     LGLGGILAAAIGRVAFRDPVGVGAINGEVGNVTGMAQLIFGRYVWVFEMTSALLITAA
                     LGAMVLAHRERLGRAPNQREWSTRRFRSGEHLAGLPVPGVYARHNAVDTPALLPDGTP
                     SELSVSRVLTARDQNQSPTPFIEAEAERDAEIAKGEER"
     gene            complement(1822..2439)
                     /gene="nuoI"
                     /locus_tag="PL014_08810"
     CDS             complement(1822..2439)
                     /gene="nuoI"
                     /locus_tag="PL014_08810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOT57230.1"
                     /translation="MADEQKKKSGFFSDLFAPVGGFGVTFSTMFRKVITEEYPEEKRP
                     TQPRFHGRHQLNRHPDGLEKCVGCELCAWACPADAILVEGAGNDDSEGGSGRFSPGER
                     YGRVYQINYLRCIFCGLCIEACPTRALTMTNEYELADNNRADLIFTKDQLLAPVQQGM
                     LTPPFPMAPGMEERDYYLGKVSAATDAQRAYVAEHAHPTDAEVTS"
     gene            complement(2432..3817)
                     /gene="nuoH"
                     /locus_tag="PL014_08815"
     CDS             complement(2432..3817)
                     /gene="nuoH"
                     /locus_tag="PL014_08815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOT57231.1"
                     /translation="MTALPELVSVASLATASVDNPKADFSDTPFWLSVVKGLMIFVYL
                     LLSTLLVIWFERRVIGRMQQRPGPNRNGPFGLLQTLADGMKAMLKEDVRPKAADVFVF
                     TLAPIITATMCFVSFSIMPLAGEVSLFGHQTPLQLTDTPVAVLLVLAVAGVGIYGVVL
                     AGWSSGSTYPLMGGLRATAQMISYEIAMGLSLVAVFMYAGSMSTSQIVSAQSDLWFAL
                     PLFFSFFVFTITMVGETNRLPFDLAEGEGELTGGFHTEYGSMRFAMFFLGEYINMFTV
                     AALATTMFLGGPAAPPGIAAINDGMFNQGWWGLLWFTAKLWVFMFFFVWLRGSLPRVR
                     YDQFMRFGWKFLIPATLTWVVLISVFRAGRNGWFDSLGTVDFANRSFPVVTLGLIGLI
                     AVVALVGVWLWDIRAEAKEAAAHPPIPDEVDPYAGGHPVPPLPGQRLVEPSLAAPALT
                     ADSETKEAVRG"
     gene            complement(3823..6342)
                     /locus_tag="PL014_08820"
     CDS             complement(3823..6342)
                     /locus_tag="PL014_08820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HOT57232.1"
                     /translation="MTTTPKTAPPAAGAPAAPAAAPPAADQVTLTIDDREVSVAKGTL
                     IIRAAEQAGIEIPRFCDHPGLDPVGACRQCLVEVWMPGRPGPDGTPGEPTQMQGPPGR
                     MKPQASCTMTVAPGMVVKTQYTSEGADKAQQGIMEFLLINHPLDCPVCDKGGECPLQN
                     QAMSNGRATSRFEDIKRTYPKPINISSQVLLDRERCVLCARCTRFSDQIAGDPFIALV
                     ERGALQQVGIYEEQPFESYFSGNTVQICPVGALTGAAYRFRSRPFDLVSTPSVCEHCA
                     SGCAQRTDHRRGTVLRRMAQADPLVNEEWNCDKGRWAFAYTGVGDRIEFPMVRGTDGE
                     LKVVSWRDALAAAAAGLRGATASGVLVGGRVSTEDAYAYGKFARTVLGTNDVDFRARP
                     HSAEEADFLASHVVATSPEGGSVTYGDLETASTVVLVGLEPEDESPMVFLRLRKAFRK
                     SGTTVYAVSPVATRGLAKVGGTLIPTAPGTEPEVLAALAAGSGSDVVTAASAALRENA
                     VILVGERLASVPGALSAVAELAETTGARVAWVPRRAGERGALEAGALPTLLPGGRPAS
                     DAAARAAVATAWGVQSLPEVAGRDTAAILAAAASGELNALVVGGVDPADIGDAVASAA
                     LERAFVVSLDLRPSAVTAVADVVLPVAAHAEKGGTFVDWEGRAREFEMAVETGFDSDY
                     RVLDMLADEMGDFLGTRTLREVRTEMSSLAAWDGARAAAPTVPAGAVPVVEPGTLVLA
                     TWRHLLDAGSLQDGEPFLAGTAPAPRARVSVATAADLGVVDGDHLTVRAGDQSVTAPV
                     LVSEMADHVVWLPTNSAGCDLRGALEGAPGALVSVTKEALA"
     gene            complement(6339..7661)
                     /gene="nuoF"
                     /locus_tag="PL014_08825"
     CDS             complement(6339..7661)
                     /gene="nuoF"
                     /locus_tag="PL014_08825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOT57233.1"
                     /translation="MATTLTPILSAFWDDPKSWSLETYTRHNGYKALDKALAMEPAAI
                     IAAVKDSSLRGRGGAGFPTGMKWSFMAPPDGGPRYLVVNADESEPGTCKDIPLMMANP
                     HVLIEGVAISSFAIGCEHAFIYLRGEVVHVYRRLLRAVEEAYAAGHLGKNVHGTGVNL
                     DITVHAGAGAYICGEETALLDSLEGRRGQPRSKPPFPGAAGLYARPTSVNNVESIASV
                     PGIILEGVDWFTGMGTEKSTGFGIFSLSGHVKTPGQYEAPLGITLRELIDMAGGMRDP
                     DKALKFWTPGGSSTPLFTAEHLDVPLDFESVGAAGSMLGTRALQIFDETVCVVRAVDR
                     WTEFYKHESCGKCTPCREGTWWLKQITGRLERGEGTEEDIDKLLDLCDNILGRSFCAL
                     ADGATSPVMSSIQYFRDEYIAHLTHGGCPFDPKASTLFAKDAKDRVNA"
     gene            complement(7661..>8361)
                     /locus_tag="PL014_08830"
     CDS             complement(7661..>8361)
                     /locus_tag="PL014_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT57234.1"
                     /translation="QYKRHPNGEYTVGVCTNTLCAVMGGDEIWDAVSGHLGVGHDETT
                     ADGRITLERVECNAACDYAPVVMANWEFFDNQTPESTIALVDDLTAGKPVAPTRGPTS
                     LCTFKQVSRVLAGFHDGLADEGVGAGPASLAGLKLAHEQGWTAPEGASATAAAPAAPE
                     PAASEAPAPAAAAEPGNTEVTPASDEAATAKVEPAATRDPAEQAPAEDDAKARTTGAE
                     KAPRKTSAPKKKGS"
BASE COUNT         1339 a         2723 c         3117 g         1182 t
ORIGIN      
        1 aactgggccg acttgccgca ggcaccgacg agcagcagca ggccgatcgc ggtgatggcc
       61 ccctggttgg cggtggcgac cccctcgttg accgtggcgt agtcgacccc accgaaggtc
      121 acgaacatga ccatgatggc gatcgacagg ccgaggtcac cgacgcggtt gacgaagaag
      181 gccttgttcg cggcactcgc gtacgccggg ttccagttcc agaagccgat gagcaggtag
      241 gacgcgagac cgacgccctc ccagccgacg aacagcagga ggtaggagtc cgcgagcacc
      301 agcaggagca tcgaggcgat gaacaggttg aggtacgcga agaagcgccg cttgtcgggg
      361 tcgtgctcca tgtacccgat cgagtacacg tggatcagcg acccgacgaa cgtgatgagc
      421 atgacgaagg tcagcgacag cgggtcggcg agcagcccga ggtcgagctg gaaggacccg
      481 gcgggaaccc agttccacag cgtcaggtgc tgcgcccgct cctcggtggg caggccgatg
      541 aggtgcagga tgatggcgac gccgacgagg aaggatcccc aggacatggc ggtcgcgagc
      601 agcggaccga acttgtccgt tcgacgcccg ccgagcagca gcacggccgc accgagcagg
      661 ggcagggcga tgagcagcca cacgaacgcg gatgcgccgc tcgcgtcgac tggggccagc
      721 ccgctgccct cggtgatgag tgaggtcact ggtgctcctt acagcttcag caggttggcg
      781 tcgtcgaccg aggccgagcg gcgggagcgg aagatggcca tgatgatggc caatccgacg
      841 acgacttcgg ctgccgcgac gaccatgacg aacagggcga tgacctgacc gtcgaggttg
      901 ccgttcatcc gggcaaaggt cacgaaggcg aggttcgcgg agttgagcat gagctcgacc
      961 cccatgaaga cgatgatcgc gttgcgcctg gtgagcacca cggccgcacc gatgacgaac
     1021 aggatcgtcg cgaggtagat gtagttgacg gggttcatcg ctcctccccc ttggcgatct
     1081 cggcgtctcg ttcggcctcg gcctcgatga acggcgtcgg cgactggttc tggtcgcggg
     1141 cggtgagcac gcgggagacg gagagctcgg acggggtgcc gtcaggcagc agggccgggg
     1201 tgtcgaccgc gttgtggcgg gcgtagacac cgggaaccgg caggccggcg aggtgctcac
     1261 cggagcggaa gcgacgggtg ctccactcgc gctggttcgg cgcccgaccg agccgctcgc
     1321 ggtgggccag caccatggca cccagggccg cggtgatgag cagcgcactc gtcatctcga
     1381 agacccagac gtagcggccg aagatcagct gcgccatccc ggtgacgttg ccgacctcgc
     1441 cgttgatcgc accgacgccg accgggtcgc ggaaggcgac ccgcccgatg gcagcggcga
     1501 ggatgccgcc gaggcccagg cagaggaccg cggtcaccca gcgctggccg ggcagggtct
     1561 ccacgaggct gtcggaggag tcgacgccga cgagcatgat gacgaagagg aacagcatca
     1621 tgacggcgcc ggtgtagacg aagatctgga tgacaccgag gaagtcggca ccctgggcga
     1681 tgtagaagat gccgaggatg atcatcgtca gggccatgcc gagcgccgcg tggacggcct
     1741 tcttggcaaa gaccagaccg agggagccca ggaccgcgag gggtccgagc acccagaaga
     1801 gcacctgctc accggtgccg gtcacgaggt cacctccgcg tcggtggggt gggcgtgctc
     1861 ggcgacgtat gcacgctgcg cgtccgtggc tgccgacacc ttcccgaggt agtagtcgcg
     1921 ctcctccatg cccggcgcca tggggaacgg cggggtgagc atgccctgct gcaccggggc
     1981 cagcagctgg tccttggtga agatcaggtc ggcccggttg ttgtcggcga gctcgtactc
     2041 gttggtcatc gtcagcgcgc gcgtcgggca ggcctcgatg cacaggccgc agaagatgca
     2101 ccgcaggtag ttgatctggt agacgcggcc gtagcgctcc ccggggctga accggccgga
     2161 accaccctcg gagtcgtcgt tgccggcccc ttcgacgagg atcgcgtcag cggggcaggc
     2221 ccaggcgcac agctcgcagc cgacacactt ctcgagaccg tcaggatgcc ggttgagctg
     2281 gtggcgtccg tggaaccgag gttgggtggg gcgcttctcc tcggggtact cctcggtgat
     2341 gaccttgcgg aacatcgtcg agaaggtcac cccgaatccg cccacggggg cgaacaggtc
     2401 ggagaagaag ccggacttct tcttctgttc gtcagccacg aacggcctcc ttggtctcac
     2461 tgtccgcagt cagggccggg gcggcgagcg agggctcaac caggcgctgg ccgggcaggg
     2521 gtggcacggg atgtccaccc gcgtacgggt cgacctcgtc ggggatgggc gggtgggcgg
     2581 cggcttcctt ggcctcggca cggatgtccc acagccagac acccacgagg gcgaccacgg
     2641 cgatgagccc gatcaggccc agggtcacca cggggaagga acggttggcg aagtccacgg
     2701 tgcccagcga gtcgaaccac ccgttgcgcc cggcccggaa caccgagatg agcaccaccc
     2761 aggtgagggt cgcggggatg aggaacttcc agccgaagcg catgaactgg tcgtagcgca
     2821 cgcgcggcag cgagccgcgc agccagacga agaagaacat gaacacccac agcttggcgg
     2881 tgaaccacag cagaccccac cagccctggt tgaacatgcc gtcgttgatc gccgcgatgc
     2941 cgggaggggc ggcggggcca ccgaggaaca tcgtcgtggc cagcgcggcg acggtgaaca
     3001 tgttgatgta ctcaccgagg aagaacatcg cgaagcgcat ggagccgtac tcggtgtgga
     3061 acccaccggt gagctcgccc tcaccctcgg cgaggtcgaa gggcagacgg ttggtctcac
     3121 cgaccatggt gatggtgaag acgaagaagc tgaagaacag cgggagggcg aaccacaggt
     3181 ctgactgggc agagacgatc tgcgaggtcg acatcgaccc ggcgtacatg aacacggcca
     3241 cgagcgagag gcccatggcg atctcgtagg agatcatctg cgcggtcgcg cgcaggccac
     3301 ccatgagcgg gtaggtcgag ccggacgacc agcccgcgag cacgacaccg tagatgccga
     3361 cgcccgcgac ggcgagcacg agcagcacgg cgacgggggt gtcggtgagc tgcagcgggg
     3421 tctggtgccc gaacagcgac acctcaccgg ccagcggcat gatcgagaac gagacgaagc
     3481 acatcgtcgc cgtgatgatc ggcgccaggg tgaagacgaa gacgtcagcg gccttgggac
     3541 gcacgtcctc cttgagcatc gccttcatgc cgtcggccag cgtctggagg aggccgaagg
     3601 ggccgttgcg gttcgggccg ggacgctgct gcatccggcc gatgacccgg cgctcgaacc
     3661 agatgacgag cagcgtcgac agcaggaggt agacgaagat catcaacccc ttgacgaccg
     3721 acagccagaa cggcgtgtcg ctgaagtcgg ccttcgggtt gtcgacgctc gcggtagcca
     3781 gcgaggccac cgagaccagc tcgggcaggg cggtcagcgt gctcacgcga gggcctcctt
     3841 cgtgacggac accagggctc cgggggcacc ctcgagggcg ccgcgcaggt cacacccggc
     3901 actgttcgtc gggagccaga cgacgtggtc ggccatctcc gagacgagaa ccggtgcggt
     3961 gacgctctgg tcgccggccc ggaccgtgag gtggtcgccg tcgaccaccc cgaggtcagc
     4021 ggccgtggca accgacaccc gggcacgtgg tgcgggtgcg gtcccggcga ggaacggttc
     4081 accgtcctgc agcgagccgg cgtcgagcag gtgccgccag gtggcgagca ccagggtgcc
     4141 gggctcgacg acgggcacgg cacccgcagg cacggtcggc gcagcggcgc gtgcgccgtc
     4201 ccacgccgcg agcgaggaca tctccgtgcg gacctcgcgc agggtgcgcg tgccgaggaa
     4261 gtcacccatc tcgtcagcca gcatgtccag gacgcggtag tcgctgtcga aaccggtctc
     4321 gacggccatc tcgaactcgc gggcgcggcc ctcccagtcc acgaaggtcc cgcccttctc
     4381 ggcgtgcgcc gcgacgggca gcacgacgtc cgcgacggcg gtcacggccg agggccgcag
     4441 gtcgagggag acgacgaacg cgcgctccag ggcggcggac gcgacggcat ccccgatgtc
     4501 tgcggggtcg acaccaccga cgacgagggc gttgagttca ccggacgctg ccgcggcgag
     4561 gatcgccgcc gtgtcacggc cggcgacctc gggcagggac tgcacgcccc acgcggtcgc
     4621 gaccgcggcg cgggcggcgg cgtcggatgc cgggcggccg ccgggcagca gggtgggcag
     4681 cgcgccggcc tcgagcgcac cgcgctcacc ggcccgacga ggcacccacg ccacccgggc
     4741 accggtggtc tccgccagtt ccgcgacggc actgagcgca ccgggcaccg aggcgagccg
     4801 ctcgccgacg aggatgaccg cgttctcacg cagcgcggca gaggccgccg tgacgacgtc
     4861 ggagccggaa ccggccgcca gcgcggccag cacctcgggc tcggtgccgg gagcggtcgg
     4921 gatcaacgtg cccccgacct tggccaggcc ccgtgtcgcg acgggcgaca cggcgtagac
     4981 ggtggtgccg ctcttgcgga aggccttgcg cagccgcagg aagaccatgg ggctctcgtc
     5041 ctcgggctcg aggccgacga gcaccacggt gctggccgtc tcgaggtcgc cgtaggtcac
     5101 ggagccaccc tcgggggacg tcgcgacgac gtgcgaggcg aggaagtcgg cctcctcggc
     5161 cgagtgcgga cgggcgcgga agtcgacgtc gttcgttccg agcacggtgc gggcgaactt
     5221 gccgtaggcg taggcgtcct cggtgctgac gcgcccaccg acgagcacgc cggatgccgt
     5281 ggcgcctcgc aggcccgcag cggcggcggc cagcgcatcg cgccacgaga cgaccttgag
     5341 ctcaccatcg gtgccgcgca ccatcgggaa ctcgatgcgg tcaccgacgc cggtgtaggc
     5401 gaacgcccag cggcccttgt cgcagttcca ctcctcgttc accagcgggt cggcctgggc
     5461 catgcggcgc aggaccgtgc cgcggcggtg gtcggtgcgc tgggcgcacc cgctggcgca
     5521 gtgctcgcag acgctgggcg tcgagacgag gtcgaacggg cgggagcgga agcggtaggc
     5581 ggcaccggtg agggccccca cggggcagat ctgcaccgtg ttgccggaga agtagctctc
     5641 gaagggctgc tcctcgtaga tgccgacctg ctggagggcg ccgcgctcga ccagcgcgat
     5701 gaacgggtca cccgcgatct ggtcggagaa gcgggtgcag cgcgcgcaca ggacgcagcg
     5761 ctcgcggtcg agcaggacct gcgaggagat gttgatcggc ttggggtagg tgcgcttgat
     5821 gtcctcgaag cgcgaggtcg cccgaccgtt gctcatcgcc tggttctgca gggggcactc
     5881 gccgcccttg tcgcacaccg ggcagtcgag cgggtggttg atcagcagga actccatgat
     5941 cccctgctgg gccttgtcgg caccctcgga cgtgtactgc gtcttgacga ccatgccggg
     6001 ggcgacggtc atcgtgcagg acgcctgcgg cttcatccgg ccgggagggc cctgcatctg
     6061 ggtcggctcg ccgggcgtgc cgtcggggcc gggccgaccc ggcatccaca cctcgacgag
     6121 gcactgccgg cacgcgccga ccgggtccag gcccgggtgg tcgcagaacc gcgggatctc
     6181 gatgccggcc tgctcggccg cgcggatgat gagggttccc ttggcgaccg agacctctcg
     6241 gtcgtcgatg gtcagcgtga cctggtcggc tgcgggcggg gccgccgcgg gtgcagcggg
     6301 tgcaccggct gccggaggcg cggtcttggg cgtggtggtc atgcgttcac gcggtccttt
     6361 gcgtccttgg cgaacagggt cgacgccttc gggtcgaacg ggcagccacc gtgggtgagg
     6421 tgcgcgatgt actcgtcgcg gaagtactgg atggagctca tcaccgggct cgtcgcgccg
     6481 tcggcgaggg cgcagaagct gcggccgagg atgttgtcgc agaggtccag cagcttgtcg
     6541 atgtcctcct cggtgccctc accgcgctcg agccgtccgg tgatctgctt gagccaccag
     6601 gtgccctcgc ggcacggggt gcacttgccg cacgactcgt gcttgtagaa ctcggtccac
     6661 cggtcgaccg cgcgcacgac gcacacggtc tcgtcgaaga tctgcagggc ccgggtgccg
     6721 agcatcgagc ccgcggcccc gacggactcg aagtccagcg gcacgtcgag gtgctcggcg
     6781 gtgaacagcg gcgtcgagct gccgccgggg gtccagaact tcagggcctt gtcggggtcg
     6841 cgcatgccgc cggccatgtc gatcagctcg cgcagcgtga tgccgagggg cgcctcgtac
     6901 tggccggggg tcttgacgtg cccggacagg ctgaagatcc cgaagccggt ggacttctcg
     6961 gtgcccatcc cggtgaacca gtcgacgccc tcgaggatga tgccgggcac cgaggcgatg
     7021 gactcgacgt tgttgaccga ggtcgggcgg gcgtacaggc ccgcagcacc cgggaacggc
     7081 ggcttgctgc ggggctggcc gcgccggccc tcgaggctgt ccaggagggc ggtctcctcg
     7141 ccgcagatgt aggcgcccgc gccggcgtgc acggtgatgt cgaggttgac ccccgtgccg
     7201 tggacgttct tgccgaggtg tccggccgcg taggcctcct ccaccgcgcg cagcaggcgg
     7261 cggtagacgt gcacgacctc gccgcgcagg tagatgaagg cgtgctcgca gccgatggcg
     7321 aacgagctga tcgcgacacc ctcgatgagg acgtgcgggt tggccatcat gagcgggatg
     7381 tccttgcacg tgcccggctc ggactcgtcg gcgttgacga cgaggtagcg gggaccgccg
     7441 tccgggggtg ccatgaacga ccacttcatg ccggtcggga agccggcgcc gccgcggccg
     7501 cgcagcgagg agtccttgac ggcagcgatg atcgccgccg gctccatcgc gagcgccttg
     7561 tcgagcgcct tgtagccgtt gtggcgggtg taggtctcca gggaccagga cttcgggtcg
     7621 tcccagaacg cactgaggat cggggtgagc gtggtggcca tcacgaaccc ttcttcttcg
     7681 gggcggaggt cttgcgcggt gccttctcgg ccccggtggt gcgggccttg gcgtcgtcct
     7741 cggcgggagc ctgctccgcc gggtcgcggg tcgcggccgg ctcgaccttg gcggtggccg
     7801 cctcgtcgct cgcgggggtg acctcggtgt tgccgggctc ggccgcggcc gccggggcag
     7861 gtgcctcgga tgccgccggc tcgggtgccg ctggtgcggc ggccgtggcc gacgcacctt
     7921 cgggtgcggt ccaaccctgt tcgtgcgcga gcttcagtcc ggcgagcgac gccggaccag
     7981 cgccgacccc ttcgtcggcc agcccgtcgt ggaagcccgc cagcacgcga ctgacctgct
     8041 tgaacgtgca cagggacgtc ggcccgcggg tgggcgcgac gggcttgccg gcggtgaggt
     8101 cgtcgaccag ggcgatggtg gactcgggcg tctggttgtc gaagaactcc cagttcgcca
     8161 tgacgaccgg ggcgtagtcg caggccgcgt tgcactcgac gcgctcgagc gtgatcctgc
     8221 cgtccgcggt ggtctcgtcg tggccgacgc cgaggtgacc cgacacggca tcccagatct
     8281 cgtcgccgcc catgacggcg cacagggtgt tggtgcagac gccgacggtg tactcgccgt
     8341 tggggtggcg cttgtactgc g
//
LOCUS       DAOSOC010000108         8307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_8546_length_8307_cov_10.738003, whole genome shotgun sequence.
ACCESSION   DAOSOC010000108 DAOSOC010000000
VERSION     DAOSOC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8546_length_8307_cov_10.738003"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PL014_08835"
     CDS             <1..196
                     /locus_tag="PL014_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT57235.1"
                     /translation="LDRLLEERGMTLAALADAVGVTVVNLSVLKNNRAKAVRFSTITA
                     ICDVLECQVGDVLSVEREVR"
     gene            complement(224..1423)
                     /locus_tag="PL014_08840"
     CDS             complement(224..1423)
                     /locus_tag="PL014_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT57236.1"
                     /translation="MSPLASYRRLFSLAGPLYVAIAFIGRLPLAMAQMGTLLLVAGTT
                     GSYGTGGAAAGALAVTNAIGAPIAGALADRVGQRPVVFVQSVLGGLGLIALVVLAHAQ
                     VTPWIVILTAALAGAAMPQIGPLARVRWRPITRNQKHHQPRLMEAAFSYEGAADEASF
                     VLGPALLGLVVVLASPELALLIAAAILLVFGCAFALHPTARLTHAHRPDAAATGRLVT
                     GTFAILLVAQLLIGVVFGSIQTGTTVLTTDAGQSGLAGLVHACLGVGSVIAGLAVAAL
                     PARFGYGSRVLVAALGLALLSLPLLVTDTVPQLFAVVLVLGFVVAPYMISVFMLGERA
                     APVERVGAAMTLLAGATGLGYALGSATAGRLADMHGHPGAFAVTVGAALTAAALATAS
                     QPALRRV"
     gene            complement(1717..4674)
                     /locus_tag="PL014_08845"
     CDS             complement(1717..4674)
                     /locus_tag="PL014_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT57237.1"
                     /translation="MTTLARPGGAPSSGADEHPDAPRHAAEAPAEISRFAAWRASWAV
                     ALRMARRDVRRHKGRSALIIVMVTVPTALLSLVLTLALTSDIRGAETIPMLMGNGQAL
                     IEGPDSSRVLQGPDPARGFGGSEGAARPIPGYDPERSGEENSEAIAALIGGAPVSAVR
                     HFDARLTVDERRVRVSALALDGRVGLGDKLELVSGRWPAGADEALVTASGVDSGLPST
                     GQQTLSIAGSDRTLAIVGVARAASLYDTVGLVTAVEPPDLEQSYGGWIVRGTDPVTWA
                     DVRTLNEYGLRVSSASVLRDPPAISELPAEVQESASGEDSQMRLIAGMGGAMLLIITT
                     LLVGPAFAVSAARQRRTLALAASNGATTAVLRRTVLASALVLGATSALAGTALGVALT
                     PLLIRGLNRWADYGVDAPLDIRWVLLGGIALCAVVSTLIAALLPARRLGRLDIVGVMR
                     GQAVSPPPSKVVLAVGAVLSALGALVILGSTGVAGLPSVTRLLGIQTGGDYAVTVGAV
                     VLIVGSLFLVPVALAGIGRLGSHLPTTLRIAARDLARHRSRSAPSVAAVLAAVAGLSF
                     GLTGLASDTEESRRTYIPVTLPGEVVVRAWAEPITADAIRAAAPGLIVTENVAAGAVD
                     PMNGLDAVPTEPYRLEFVSIVPPGCTAARTISDPEWDAAENARYLAAEEAGTTDSFVF
                     GTAPCSTGGTTYGGTGSTLLLPAEEIVRRLGLDAAQADAVRAGAVITRALSPATASGD
                     QVRVARGTFVIDPNATGPTDPQVEVLQEASLPAIQLAESAQTEGKMLGASLMLASDSV
                     VTTGWPSRPESFTVRDAAGAPVTQEAVERLRAALGDGFEVSVERGFTRDDRIVVAALL
                     GVFALLILVITLTSTALTVAEQQSDQATLAALGATRGTRRLMAGAAAFLLAAVGCVLG
                     VAVGLVPGIAISRALTSAGWDPVNGVPEGQETILVIPWLSLLIVGVLVPMVAGAIAWA
                     GIRKAPQITRRST"
     gene            complement(4671..5402)
                     /locus_tag="PL014_08850"
     CDS             complement(4671..5402)
                     /locus_tag="PL014_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57238.1"
                     /translation="MSTPHVVLTDVTRIHGDRNMAVVALAGVSLDIHLGELVAVMGPS
                     GSGKSTLLNVAGGLDRPTSGTVTIAGDRLTDLAPRDLAKLRRRRMGFIFQDYNLIPSL
                     TAAENVALPLELDGTRAGQARRQARRTLDLMNLMELSDRFPDQMSGGQQQRVAIARAL
                     VGSRRFVLADEPTGALDSHTGEEVLRVLRERCDEGVAGLLVTHEARHAAWADRVVFLR
                     DGVIVDSTGHRETPEDLLVAGEAAR"
     gene            complement(5399..6112)
                     /locus_tag="PL014_08855"
     CDS             complement(5399..6112)
                     /locus_tag="PL014_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT57239.1"
                     /translation="MSVRSGLMALLSEGEKYGAQLRTEFEARTGGTWPLNVGQVYTTL
                     DRLVRDGLVAQASEPDAEGRIAYRLTSTGRQVLDAWWTTPVDRTNTPRDELTIKLALA
                     VTAPGVDVPRVVQAQRTATLQHLRDLTRLKREALGSTEGAAEGPRAVEDPIAWLLVLE
                     NLIFAAEGEARWLDHVETVIARRRPRPEYADPHRTPDHQHPAPHPRGADPASDTTASG
                     PTHHHASATHHTHTGGNQS"
     gene            complement(6266..6628)
                     /locus_tag="PL014_08860"
     CDS             complement(6266..6628)
                     /locus_tag="PL014_08860"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HOT57240.1"
                     /translation="MSTVALVLTGLVALIHVYIVLLEMLWWDTPRGRAAFGTSRELAS
                     QTKVLAANQGLYNGFLVAGLLLAMVGPSEHRFAFAVFSLGCVIVAGLYGAATASRKIL
                     FVQTVPAALALAAQFLAR"
     gene            complement(6652..7629)
                     /locus_tag="PL014_08865"
     CDS             complement(6652..7629)
                     /locus_tag="PL014_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOT57241.1"
                     /translation="MKAITIPEFGGPEAMVLSEVPDPEPRAGEVLVRVAAAGVNRADV
                     LQRQGHYAPPPGESDVPGLEVSGTIEALGDGVEGWSVGDEVCALLSGGGYAELVRVPA
                     GQLLPVPAGVSLINAAALPEVTCTVWSNVFLTANLQPGETLLVHGGSSGIGTMAIQLG
                     RAIGAVVAVTAGSPEKLAACADLGAEVLVNYREEDFVERVMAATDGAGADVVLDNMGA
                     KYLARNVAVLATGGRLVVIGMQGGTRAELDLGVLLRKRAAVIATSLRARPLGEKATIV
                     AAVREHVWPLIEAGEVRPVVMSRHPLADAAAAHRELEASAHIGKILLTP"
     gene            complement(7696..>8307)
                     /locus_tag="PL014_08870"
     CDS             complement(7696..>8307)
                     /locus_tag="PL014_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT57242.1"
                     /translation="YVRHLPEARRYGLGLAAYELGSAYQRQAPLERLARGVMDHLVDA
                     TGHNAHLAVLHGRDVLYVIEQRATGRPLLVTDVGVRLPAVLTASGLAMLAALPPAQVR
                     ALFPDRTALVQRDGRGPASTTRLRAELQAVRQRGHAVEDGLVTPGIGSVAVAVLDHND
                     YPVAGVAVSYPQADVDAVAAAALVRAVTQAAGALGRRLGRAAR"
BASE COUNT         1237 a         2846 c         3117 g         1107 t
ORIGIN      
        1 cctcgaccga ctcctcgagg agcgcggcat gaccctggcg gccctcgcgg atgccgtggg
       61 cgtcaccgtc gtcaacctct cggtgctcaa gaacaaccgg gccaaggccg tccggttcag
      121 caccatcacg gcgatctgcg acgtgctcga gtgccaggtg ggggacgtgc tgagcgtcga
      181 gcgcgaggtg cgctgacggc atccgatcac cgggcgcgaa gggtcagacg cggcgaaggg
      241 cgggctggga tgccgtcgcg agggctgccg ccgtcagcgc cgctcccacg gtcacggcga
      301 acgcgccggg gtggccgtgc atgtcggcga ggcgtccggc ggtggccgag ccgagggcgt
      361 acccgagccc ggtggccccg gcgagcaggg tcatcgccgc accgacccgc tcgacggggg
      421 cagcgcgctc gcccagcatg aagacgctga tcatgtaggg ggcgacgacg aagcccagca
      481 cgaggacgac ggcgaacagc tgcggcaccg tgtcggtgac gagcagcggc aaggacagca
      541 gggccaggcc cagggcggcg acgagcacgc gggagccgta tccgaagcgg gcgggcaggg
      601 ccgcgacggc gagcccggcg atgaccgatc cgacaccgag gcaggcgtgg accagccccg
      661 cgaggccgct ctgcccggca tccgtggtga gcaccgtcgt gccggtctgg atcgacccga
      721 acaccactcc gatgagcagc tgcgcgacga gcaggatcgc gaaggtgccg gtgacgagac
      781 ggccggtggc ggcggcatcc gggcggtggg cgtgggtgag gcgggcagtg gggtgcagcg
      841 cgaacgcgca cccgaacacg agcaggatcg cggcggcgat gagcagggcg agctcggggc
      901 tggccagcac gacgacgagg ccgagcagtg ccgggccgag cacgaaggag gcctcgtcgg
      961 cggcgccctc gtaggagaag gcggcctcca tcaggcgcgg ctggtggtgc ttctggttgc
     1021 gggtgatggg gcgccagcga acccgtgcga gcgggccgat ctgcggcatc gcggcgccgg
     1081 cgagggcggc ggtgaggatg acgatccacg gggtgacctg cgcgtgggcg agcacgacga
     1141 gggcgatcag cccgagcccg ccgagcaccg actggacgaa cacgacgggc cgttgcccga
     1201 cgcggtcggc cagggccccg gcgatcgggg caccgatggc gttggtcacg gccagggcac
     1261 cggcggcggc gccaccggtg ccgtaggagc cggtggtgcc ggcgacgagc agcagggtgc
     1321 ccatctgggc catggccagg ggcagtcggc cgatgaaggc aatggcgacg tagagcgggc
     1381 cggcgaggct gaacagccgc cggtaggagg cgagggggga catgcgcggc ttcctggtct
     1441 gcgcccgggg agggcgaaac gagtgtgcgc cgaacccgcc tccaaggacg caccaggacc
     1501 ctgtgctccg cccatcctac cggctcgcgt ccgcagccac cccgcctgaa ctttctcgcc
     1561 cggtgccaga aacttcaggt tcgcaccact aatgaagtgg tgtcatggtg aagctggtgg
     1621 catcgggcga gaaagttccg acgggggttc gggtctgccg cgcgcggttg gccgcttcgc
     1681 aggtggctgg agcgcaataa gtcgcggagg agggggtcag gtggaacggc gggtgatctg
     1741 cggggccttg cggatgccgg cccacgcgat cgcgcccgcc accatcggta ccagcacgcc
     1801 cacgatgagc agagagagcc acgggatgac gaggatcgtc tcctgtcctt cgggcacacc
     1861 gttgacggga tcccacccgg cgctggtgag cgctcgtgag atcgcaatgc ccgggacgag
     1921 gccgaccgcg accccgagca cgcacccgac cgcggcgagc aggaaagcgg cggccccggc
     1981 catgagtcgc cgggtgcccc gagtggcccc gagcgctgcc aaggtcgcct ggtcactttg
     2041 ctgctcggcg acggtgagag ctgtcgaggt gagcgtgatg acgagaatga gcagggcgaa
     2101 gacccccagc agggcagcga ccacgatgcg gtcgtcgcgg gtgaaccccc gttccacgga
     2161 gacctcgaac ccgtcgccga gtgccgccct caaccgctcc accgcttcct gggtgacggg
     2221 cgcacctgcc gcgtcccgca ccgtgaagct ctcgggccga gagggccacc cggtggtcac
     2281 gacggagtcc gatgccagca tgaggctggc accgagcatc ttgccctcgg tctgcgctga
     2341 ctcggccagt tggattgcgg gaaggctggc ctcctggagc acctcgacct gtgggtcggt
     2401 gggcccggtg gcgttggggt cgatgacgaa ggtgccccgc gccaccctca cctggtcgcc
     2461 ggacgcggtg gcgggtgaca gggcgcgggt gatgacggct ccggcgcgca cggcgtccgc
     2521 ctgagcggcg tcgaggccga ggcgtcgcac gatctcctcg gcagggagca gcaaggtgga
     2581 gcccgtgccg ccgtatgttg ttccgccagt cgagcagggc gcagtgccga acacgaagga
     2641 atcggtggtg ccagcctcct cagcagccag gtatcgcgca ttctcggcag cgtcccactc
     2701 ggggtccgag atggtgcggg ctgcggtgca cccgggcggg acgatggaga cgaactcgag
     2761 ccggtagggc tcggtcggga cggcgtctaa accgttcatc gggtccacag cgccggctgc
     2821 gacgttctcg gtgacgatca ggcccggtgc cgcggcgcga atggcgtcgg cggtgatcgg
     2881 ttcggcccat gctcgcacca ccacttcgcc gggaagggtg accgggatgt acgtgcgccg
     2941 actttcctcg gtgtcgctcg cgagcccagt aagcccaaag ctcagtccgg cgaccgccgc
     3001 caggaccgcc gcgaccgagg gtgcgctgcg cgaacgatgg cgcgcgaggt cccgggccgc
     3061 tatccgcagc gtggtcggga ggtgcgagcc gagccgaccg atccctgcca acgcgaccgg
     3121 tacgaggaag agtgacccga cgatgagaac gacggcgcca acggtgacgg cgtagtcgcc
     3181 tccggtctgg atgccgagga gtcgggtcac gctcggcagg cccgcgacgc ccgtggaccc
     3241 gaggatgacc agggcgccca gggcgctcag gacggcaccg acggcgagca ccaccttgct
     3301 cgggggaggc gagaccgcct gacctcgcat gacgccgacg atgtcgaggc gcccgagtcg
     3361 ccgtgcgggc aagagggccg cgatgagggt gctaacaacc gcgcagaggg cgatgccacc
     3421 gagcagcacc catcgaatgt ccagcggcgc gtcgacgccg tagtcggccc agcggttcag
     3481 gccgcggatg agcagcgggg tcagggccac cccgagagcc gttccggcga gggcggacgt
     3541 ggccccgagc acgagcgcag aggccagcac ggttcggcgc agcactgcgg tggtcgcgcc
     3601 gttgctcgcc gcgagcgcca acgtgcggcg ctggcgcgcg gcactgacgg cgaatgccgg
     3661 cccgacgagc agggtggtga tgatgagcag catcgcgcca cccatcccgg cgatgagtcg
     3721 catctggctg tcctcgccgg aggccgactc ctgcacctct gctggcaact cactgatcgc
     3781 cggcggatcg cgaaggacgg atgcggacga gacgcgcagg ccgtattcgt tgagggtgcg
     3841 cacatccgcc caggtcaccg ggtccgtgcc gcgcacgatc cacccgccgt aggactgttc
     3901 cagatcaggt ggctcgacag ccgtcacgag ccccacggtg tcgtacaggc tcgctgcccg
     3961 ggccacgccg acgatcgcga gcgtgcggtc agaccccgcg atggagagcg tctgctgccc
     4021 ggtggagggc aggccgctgt cgactccgga tgccgtgacg agtgcctcgt cggctcccgc
     4081 cggccagcgc ccggagacca gctcgagctt gtcgccgagc ccgacacgac cgtccagcgc
     4141 gagtgcgctc acgcggacac ggcgctcgtc gacggtcagc cgggcgtcga agtgccgcac
     4201 ggcagagact ggtgctcccc cgatgagtgc cgcgatggcc tcggagttct cctcgccgga
     4261 tctctccggg tcgtacccgg ggatgggtcg cgccgcaccc tcggacccgc cgaaccccct
     4321 ggcgggatct gggccctgga ggacacggct ggagtccggg ccttcgatga gcgcctgccc
     4381 gttgcccatg agcatcggga tggtctcggc gccgcggatg tcggacgtca gggcgagagt
     4441 cagcaccagg ctcagcaggg ccgtcggcac ggtgaccatg acgatgatca gggcgctacg
     4501 gcccttgtgg cggcgcacgt cgcgccgggc catacgcagg gcaaccgccc agcttgcccg
     4561 ccaggcggcg aaccggctga tctcggcagg tgcctcggcg gcgtggcgag gcgcatcggg
     4621 gtgctcgtcc gcgccgctgc tcggagcgcc ccccgggcgg gccagcgtgg tcatcgcgct
     4681 gcctcgcccg cgacgagcag gtcctccggg gtctcgcgat ggccggtgct gtcgacgatg
     4741 accccgtccc gcaggaacac gacccgatcg gcccaggcgg cgtgccgcgc ctcgtgggtg
     4801 acgagcaggc cggccacgcc ctcgtcgcac cgctcgcgca gcacccgcag cacctcctcg
     4861 ccggtgtggc tgtcgagggc tccggtcggt tcgtcggcga gcacgaagcg gcggctgccg
     4921 accagtgccc gtgcgattgc gacgcgctgt tgctgcccac cggacatctg gtcggggaag
     4981 cggtcggaga gctccatcag gttcatcagg tcgagggtcc ggcgggcctg ccgcctggct
     5041 tggccggcgc gggtgccgtc gagctcgagc ggcagggcga cgttctccgc cgcggtcagg
     5101 ctcgggatga ggttgtagtc ctggaagatg aagcccatgc gccgacgccg cagcttggcg
     5161 agatcgcgcg gcgcgaggtc ggtcagccgg tcgccggcga tggtgacggt cccgctcgtc
     5221 ggtcggtcga ggccgcccgc gacgttgagc aaggtcgact tgcccgaacc gctcggcccc
     5281 atgacggcga cgagttcgcc gaggtggatg tcgaggctga ccccggcgag cgcgacgacc
     5341 gccatgttcc ggtcgccgtg gatgcgcgtg acgtccgtga ggacgacgtg tggtgtgctc
     5401 atgactggtt tcccccggtg tgcgtgtggt gagtcgcgct ggcgtggtga tgggtggggc
     5461 cggatgccgt ggtgtcggat gccgggtcgg caccgcgcgg gtggggggcc gggtgctggt
     5521 ggtccggcgt cctgtgcgga tcggcgtact caggccgagg gcgccgtcgg gcgatgaccg
     5581 tctcgacgtg gtcgagccat cgggcctcgc cctcggccgc gaagatcagg ttctccagca
     5641 cgaggagcca agcgatggga tcctccacgg ccctcgggcc ctcggcagcc ccctccgtac
     5701 tgccgagggc ctctcgcttg aggcgggtga ggtctcgcag gtgctgcagc gtggccgtgc
     5761 gctgggcctg gacaacccgg ggcacgtcga ccccgggggc ggtcacggcg agggcgagct
     5821 tgatcgtcag ctcgtcgcga ggagtgttgg tgcggtcgac cggggtggtc caccaggcgt
     5881 cgagaacctg ccggcctgtg gaggtgagtc ggtacgcgat gcgcccctcg gcatccggct
     5941 cactcgcctg tgccacgagg ccatcgcgca cgagtcggtc gagcgtggtg tagacctgtc
     6001 cgacgttgag cggccaggtg ccgccggtgc gtgcctcgaa ctcggtgcgc agttgagcgc
     6061 cgtacttctc cccctcggac aacaaggcca tgagcccaga tctgacggac atcgccgccc
     6121 cctccctgcc gcggaatact cggtatgcac cccgccgtgg gacatgtata ccgagtattc
     6181 acgcccccgt caacacgccg gccccatccc gaagtcgagc agtgggggag gtggctggag
     6241 tgcaatcagt gcgggggtgt ggtggtcagc gggcgaggaa ctgcgccgca agggccaggg
     6301 ctgcgggcac cgtctgcacg aacaggatct tgcggctggc cgtcgcggcc ccgtacaggc
     6361 cggcgacgat cacacagccc aggctgaaca ccgcgaacgc gaaccggtgc tcgctcggcc
     6421 ccaccatcgc cagcagcagg cccgcgacga ggaacccgtt gtacaggccc tggttggcgg
     6481 cgagcacctt cgtctgagag gcgagctcgc ggctcgtgcc gaaggcagca cggccgcggg
     6541 gggtgtccca ccagagcatc tcgagcagca cgatgtagac gtggatcagg gccacgaggc
     6601 ccgtcaggac cagggcgacg gtgctcatgc cctgatgtct accgcatccc gtcagggggt
     6661 gagcaggatc ttgccgatgt gggcgctcgc ctcgagctcg cggtgcgcgg ccgcggcatc
     6721 agcgagtgga tgccgcgaca tcaccaccgg gcgcacctca cctgcctcga tgagcggcca
     6781 cacgtgctcg cgcacggccg cgacgatcgt ggccttctcg ccgaggggcc tggcccgcag
     6841 cgaggtcgcg atgaccgcgg cccgcttgcg cagcagcacg ccgaggtcga gctcggcgcg
     6901 ggtgccgccc tgcatcccga tgaccacgag ccggccaccc gtggcgagca ccgcgacgtt
     6961 gcgcgccagg tacttggccc ccatgttgtc gagcacgacg tccgcgcccg ctccgtcggt
     7021 ggcggccatc acccgctcga cgaagtcctc ctcgcggtag ttgacgagca cctcggcgcc
     7081 gaggtcggcg caggccgcca gcttctccgg cgacccggcc gtcacggcca caacggcgcc
     7141 gatcgcccga ccgagctgga tggccatcgt cccgatcccg gatgaccctc cgtgcacgag
     7201 cagggtctcg ccgggctgga ggttcgcggt gaggaacacg ttggaccaca cggtgcacgt
     7261 gacctcggga agcgcggcgg cgttgatgag tgagaccccg gccggcaccg gcagcagctg
     7321 accagcgggc acgcgcacca gctcggcata ccccccgccg gagagcagcg cacacacctc
     7381 gtcgccgacc gaccagccct cgaccccgtc gcccagggcc tcgatcgtgc cgctgacctc
     7441 gagcccgggc acgtcggact ccccgggcgg cggtgcgtag tggccctgcc gctgaagcac
     7501 gtcggcgcgg ttgaccccgg cggcggccac ccgcacgagc acctcaccgg cgcgcggctc
     7561 ggggtcgggc acctcgctga gcaccatggc ctcggggccg ccgaactcgg ggatcgtgat
     7621 cgccttcatg ccacccaccg tagggcgcga ccagcgtggg acatatctga cttgttggtc
     7681 acatctgtcc cacggtcagc gggcggcccg gccgagccgc cgccccagtg cccccgccgc
     7741 ctgcgtgacc gcgcgcacga gcgcggcggc ggccacggca tccacgtcgg cctgcggata
     7801 gctgaccgcc acccccgcca ccgggtagtc gttgtggtcg agcacggcga cggcgaccga
     7861 gccgataccg ggtgtcacga ggccgtcttc gacggcgtgc ccccgctgcc gcaccgcctg
     7921 cagctcggcc cgaagccgcg tcgtggatgc cgggccgcgc ccgtcgcgct ggaccagcgc
     7981 ggtgcggtcg gggaacaggg cacgtacctg tgccggggga agggccgcga gcatcgcgag
     8041 gccggatgcc gtgagcacgg ctggtagtcg cacccccacg tcggtgacca acaacggtct
     8101 cccggtggcg cgctgctcga tgacgtagag cacgtcgcgc ccgtgcagca cggcgaggtg
     8161 ggcattgtgc cccgtggcgt cgacgaggtg gtccatgacc ccacgggcca gtcgctccag
     8221 cggggcctgg cgttggtagg ccgagcccag ctcgtacgcc gcgagcccca gcccgtagcg
     8281 acgcgcctcg ggcaggtggc gcacgta
//
LOCUS       DAOSOC010000109         8086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_8876_length_8086_cov_9.877973, whole genome shotgun sequence.
ACCESSION   DAOSOC010000109 DAOSOC010000000
VERSION     DAOSOC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8876_length_8086_cov_9.877973"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PL014_08875"
     CDS             complement(<1..713)
                     /locus_tag="PL014_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOT57243.1"
                     /translation="MTGPLDGIRVIELGGIGPTPFAGLMLAELGADVVRIDRPGERNP
                     LALAAGLRRSRPSIAVDLKSPAGGDVVKRLVDGADVLVEGFRPGVAERLGLGPDECLA
                     RNPRLVHARMTGWGQTGPWAQRAGHDITYAAVSGTLHALGPAARPVTPVPVIGDFAGG
                     SMYLVVGVLAALIARGTTGRGQVVDAAMVDGAAHLLTMVHGLVGAGAWRDQREANLLD
                     GGAPFYAVYECADGKFVAVG"
     gene            complement(710..1651)
                     /gene="folP"
                     /locus_tag="PL014_08880"
     CDS             complement(710..1651)
                     /gene="folP"
                     /locus_tag="PL014_08880"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT57244.1"
                     /translation="MSDDIAVSGHVPARVPAAPAVLRLRGRTFDAGHPAVMAIVNRTP
                     DSFWSGSRHADLDSALDALDEAVARGADIVDVGGVRAGQEGPVVTAREEIERVVPFLA
                     KARDRHPDLVLSLDTWRAEVAREAAQEAGIDLVNDTWAGHDRDLVHVAAQIGAGYVVS
                     HTGGLPPRTDPVGVTYPPEPLGVVADALRTLRDGAARAVDAGVPAERVLVDPTLDFGK
                     TTAHSLTLLRHTEAVVALGFPVLQALSRKDFVGETLDLPADDRLEGTLAATAVAAWLG
                     ATVFRAHDVRATRRVVDMVASIRGDRPAAVNVRGVPA"
     gene            complement(1648..2685)
                     /gene="fgd"
                     /locus_tag="PL014_08885"
     CDS             complement(1648..2685)
                     /gene="fgd"
                     /locus_tag="PL014_08885"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HOT57245.1"
                     /translation="MSEPRGLRVGYKASAEQFAPRELAGYAVRAEELGLDSVTISDHF
                     QPWRLEGGHAPNSLAWMPWVLARTERVLVGTSVLTPTFRYNPAVLAQTFATMACLAPG
                     RVMVGVGTGEALNEVAVGSVADGQWPEFKERFGRLRESVRLMRELWTKDSVTFEGDYY
                     RTVDAAIYDRPDAPVPVYVAAGGPMVARYAGRVAQGFICTSGKGRELYADQLVPAVDE
                     GLGKAGRDLDDIDRMIEIKVSWDPDPEQALANTRFWAPLSLSAEQKHSIHSPAEMERA
                     ADELPIEQVAKRWVVASRPEDVVEALRMYTDLGFNHLVVHGPGHDQERFLSTFAEQVL
                     PGVRELVPAAR"
     gene            2776..3870
                     /locus_tag="PL014_08890"
     CDS             2776..3870
                     /locus_tag="PL014_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOT57246.1"
                     /translation="MATELITLAGHEQVVFCTDPDTGLRAIIALHDTALGPGLGGTRF
                     HPYASMDQALADVLRLSRAMTSKNAVAGLDHGGGKGVILGDPNRDKTPELLRAYGRFV
                     ESLGGRYVTACDVGTYVADMDVVGESTRWATGRSPERGGAGDSSVLTAYGVFQGMRAA
                     AQHVWGEPTLAGRRVGIAGVGKVGRRLTEQLLEDGAQVVVTDVNPDAVRDLVARHPSV
                     RAVADVDDLVREELDVYSPNALGGALDEVTVDLLRARIVCGGANNQLVSEGHGGTAER
                     LRERGITYAPDFLVNAGGVIQVSDELHGFDMERARRRTATIFDHTLEVLRSADAEDVT
                     PAVAADRLAERRIAAGGPRPWLPAGEHRDT"
     gene            4036..4230
                     /locus_tag="PL014_08895"
     CDS             4036..4230
                     /locus_tag="PL014_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HOT57247.1"
                     /translation="MGRGRAKAKQTKVARELKYFSPDTDLSALERELHGVSQTGGGRT
                     STDDREEDDDDYGSWSPGRR"
     gene            4351..5850
                     /locus_tag="PL014_08900"
     CDS             4351..5850
                     /locus_tag="PL014_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOT57248.1"
                     /translation="MTDTTVAATQRLGIETTGIEIIDESERTAQPKDLFWPWFAANVS
                     VFGISYAAFVLGFGISFWQATLVTVLGIVTSFVLCGIVAIAGKRGSAPTMILSRAAFG
                     VTGQKVPGVISWLVSIGWETFLAIMAVLATATVFEQLGWGGGTGTKVVAAVVVAAIIV
                     LASVAGYHIIMRMQSVLTWLTGAVTILFVIIASREIDWSAVAALPSGPTQSVIGAFVM
                     LMTGFGLGWINISADWSRYQHRSASGGAIVFWNTLGGALAPVLLVTFGLMLSASNEEV
                     LAGIRSDPIGTLATILPTWFLVPFLVAVIMSLISGAVLGIYSSGLTLLSLGVRVPRPA
                     AALVDGIILTLGTFFVVFVAQDFLGPFQSFLITLGVPLASWAGIMIADIALRKRDYDD
                     DALFDVRGRYGSWDWVSIATMVVASVIGWGLVVNNFAQDAAWNNWQGYLLEPLGLGTY
                     ITEGEGAPYWDGAWPYANLGVLLALVLSFAVTYVARRARVQQQEALVDG"
     gene            5843..6406
                     /locus_tag="PL014_08905"
     CDS             5843..6406
                     /locus_tag="PL014_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOT57249.1"
                     /translation="MADALARPDHPQTLGDDWLVIIDPQVVFADPQASPWGSSMWAGA
                     VGHIATLAAASTPSRTIITRFVADPGLGGSWGPYYEEWPFALVPDGDPLYAVVPELAG
                     VAEHVVTAGTFGKWPVLRDLVGDGARLTLTGVSTDCCVLSTALPAADAGATVRIAAAG
                     CAGSSEENHRRALDAMALFGPQITIVD"
     gene            complement(6458..6898)
                     /locus_tag="PL014_08910"
     CDS             complement(6458..6898)
                     /locus_tag="PL014_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57250.1"
                     /translation="MPTADLNPAVHRTSALGRRSSAARQQEPLTSRRQISDAGRAGVV
                     MAWARGALTVATGAVLVLVVARVVVGRSIFGALNLSGLPGSFATEFAGFTAAVLPGLL
                     VAWCLGFAAAATAVRATWLGARTAGLLAGALGVLAGATLFFLSR"
     gene            complement(6998..>8086)
                     /gene="purM"
                     /locus_tag="PL014_08915"
     CDS             complement(6998..>8086)
                     /gene="purM"
                     /locus_tag="PL014_08915"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOT57251.1"
                     /translation="GVDVEAGDKAVELMKASVRRATRPEVLGGLGGFAGMFDASAIAR
                     MARPVLATSTDGVGTKVAIAQALDRHDTIGFDLVGMVVDDIVVCGAEPLFMTDYIACG
                     KVVPERIAAIVSGIARACEQAGVALVGGETAEHPGLLGVDEYDVAGAATGVVEYADVL
                     GPQRVAPGDVVLGLASSGLHSNGYSLVRRVIAHAGWALDRHVDEFGRTLGEELLEPTR
                     VYSADLLALIRADGVDVHALSHVTGGGLAANLARVLPDHLHARVDRSTWTPPAIFSTV
                     GRLGSVPQADLERTLNQGVGFVAVVPASSVDAATALLEERGIRTWAMGEVHEAATAPI
                     EDGVEVVSGAKGVDGGSVQVVGSHPSSA"
BASE COUNT         1119 a         2936 c         2894 g         1137 t
ORIGIN      
        1 ccgaccgcga cgaacttccc gtcggcgcac tcgtacacgg cgtagaaggg cgccccaccg
       61 tcgaggaggt tggcctcgcg ctggtcgcgc caggcacctg ccccgacgag tccgtggacc
      121 atggtgagca ggtgcgcagc gccgtcgacc atggcggcgt cgacgacctg gccgcgaccg
      181 gtcgtgccgc gggcgatcag ggcggcgagc accccgacga cgaggtacat cgacccgccg
      241 gcgaagtcac cgatgacggg cacgggggtg accgggcgag ccgccgggcc gagcgcgtgc
      301 agcgtccccg acaccgcggc gtacgtgatg tcgtgcccgg cgcgctgcgc ccacggcccg
      361 gtctgccccc agccggtcat ccgcgcgtgg accaggcgcg ggttgcgggc caggcactcg
      421 tcgggcccga gccccagccg ctccgccaca ccgggccgga agccctcgac gagcacgtcg
      481 gccccgtcga cgagtcgctt gacgacgtcg ccgccggccg gggacttgag gtccaccgcg
      541 atgctcggcc gcgaccgccg cagccccgcg gccagggcga gcgggttgcg ctcacccggg
      601 cggtcgatcc gcacgacgtc ggcgcccagt tcggccagca tgagacccgc gaacggggtc
      661 gggccgatgc ctccgagctc gatgacccgg atgccgtcca gcggccccgt cacgcgggca
      721 cgccgcgcac gttgacggcg gcgggtcgat cgccacggat cgacgcgacc atgtcgacga
      781 cccggcgggt ggcccgcacg tcgtgggccc ggaacaccgt cgcgccgagc caggcggcca
      841 ccgcggtcgc ggccagggtg ccctcgagcc ggtcatcagc cggcaggtcg agcgtctcac
      901 cgacgaagtc cttgcgcgag agcgcctgca gcacggggaa acccagcgcc acgacggcct
      961 cggtgtgccg cagcagcgtc agcgagtgcg cggtcgtctt gccgaagtcg agcgtggggt
     1021 cgacgagcac gcgctcggcg ggcaccccgg catccaccgc ccgcgcggcg ccgtcgcgca
     1081 gcgtccgcag ggcatcggcg acgacaccga gcggctctgg agggtaggtg acgccgaccg
     1141 gatcggtgcg cggcggcagc ccgccggtgt gcgacacgac gtagccggca ccgatctggg
     1201 ccgcgacgtg cacgaggtcg cggtcgtggc ccgcccaggt gtcgttgacg aggtcgatgc
     1261 cggcctcctg cgccgcctcg cgcgcgacct cggcgcgcca ggtgtcgagg ctgaggacga
     1321 ggtccggatg ccggtcgcgc gccttcgcga ggaacggcac cacccgctcg atctcctcgc
     1381 gggcggtcac caccggccct tcctgcccgg cccggacccc accgacgtcg acgatgtcgg
     1441 ctccccgggc cacggcctcg tcgagggcgt ccagcgccga gtcgagatcg gcgtgccggc
     1501 tgcccgacca gaaggagtcg ggagtgcggt tgacgatggc catcacggcc gggtgacccg
     1561 cgtcgaaggt gcggccgcgc agccgcagca cagccggtgc agccggcacc cgtgcgggga
     1621 cgtgcccact caccgcgatg tcgtcgctca ccgggctgcc ggcaccagtt cgcgcacccc
     1681 gggcagcacc tgctcggcga aggtggagag gaaccgctcc tggtcgtgcc cgggcccgtg
     1741 gacgacgagg tggttgaagc cgaggtcggt gtacatccgc agcgcctcga cgacgtcctc
     1801 cgggcgggag gcgacgaccc agcgcttggc gacctgctcg atgggcagct cgtcggcggc
     1861 ccgctccatc tcggcggggc tgtggatcga gtgcttctgc tcagcgctga gtgacagcgg
     1921 cgcccagaac cgcgtgttcg cgagtgcctg ctcggggtcg gggtcccagc tgaccttgat
     1981 ctcgatcatc cggtcgatgt catcgaggtc gcgcccggcc ttgcccagcc cctcgtcgac
     2041 ggccggcacg agctggtcgg cgtagagctc gcgccccttg cccgaggtgc agatgaaccc
     2101 ctgcgcgacc cgccccgcgt accgggcgac catcggcccc ccggcggcga cgtacaccgg
     2161 gaccggagcg tccgggcggt cgtagatcgc ggcatccacg gttcggtagt agtcgccctc
     2221 gaacgtgacg ctgtccttcg tccagagctc acgcatgagc cgcaccgact cgcgcaggcg
     2281 gccgaagcgc tccttgaact cgggccactg accgtcggcg accgacccga ccgccacctc
     2341 gttgagggcc tcccccgtgc cgacaccgac catgacccgc cccggggcca ggcacgccat
     2401 cgtcgcgaag gtctgtgcca gcaccgccgg gttgtagcgg aaggtcgggg tgagaaccga
     2461 ggtccccacg aggacgcgct cggtgcgggc gaggacccac ggcatccacg ccagcgagtt
     2521 cggcgcgtgg ccgccctcga gacgccatgg ctggaagtgg tcggagatcg tcaccgagtc
     2581 caggccgagc tcctcggcgc gcacggcata ccccgccagc tcgcgcgggg cgaactgctc
     2641 cgctgacgcc ttgtacccga cccgcagacc gcgtggctcg ctcatggggc catcctgcac
     2701 cagccccccg accccaccga aaggttcacc agcgatagcg tgggtgacgc accgacccga
     2761 gcacgaggag cgcccatggc caccgaactc atcaccctcg ccgggcacga gcaggtcgtg
     2821 ttctgcaccg accccgacac cggcctgcga gcgatcatcg ccctgcacga caccgccctg
     2881 gggccggggc tcggcggcac ccgcttccac ccctacgcct cgatggacca ggccctcgcc
     2941 gacgtgctgc gcctgtcgcg cgcgatgacg agcaagaacg ccgtcgccgg gctcgaccac
     3001 ggcggtggca agggcgtcat cctcggtgat ccgaaccgcg acaagacccc cgagctgctg
     3061 cgcgcctacg ggcggttcgt ggagtccctc ggcggtcgct acgtcacggc ctgtgacgtg
     3121 ggcacgtacg tggccgacat ggacgtcgtc ggtgagtcga cccggtgggc caccggtcgt
     3181 tcccctgagc gtggcggcgc cggtgactcc tccgtgctca cggcgtacgg ggtgttccag
     3241 gggatgcggg cggctgccca gcacgtctgg ggtgagccga cgctcgccgg gcgccgggtc
     3301 ggcatcgccg gggtcggcaa ggtcgggcgg cgcctcaccg agcagctgct cgaggacggt
     3361 gcacaggtcg tcgtcaccga cgtgaacccg gatgccgtcc gcgacctcgt cgcgcggcac
     3421 ccgagcgttc gcgccgtggc cgacgtcgac gacctggtgc gcgaggagct cgacgtctac
     3481 agccccaacg ccctcggcgg cgcgctcgac gaggtgaccg tcgaccttct gagggcacgg
     3541 atcgtctgcg gaggcgccaa caaccagctg gtcagcgagg ggcacggggg cacggcggag
     3601 cgcctgcggg agcgcggcat cacctacgcc cccgacttcc tcgtcaatgc gggtggcgtg
     3661 atccaggtca gcgacgagct gcacggcttc gacatggagc gggcccgccg gcgcacggcc
     3721 acgatcttcg accacaccct cgaggtgctg cgcagcgcgg atgccgagga cgtcaccccc
     3781 gcggtcgccg ccgaccgcct cgccgagcgc cggatcgccg ccgggggccc gcgcccctgg
     3841 ctgccggcgg gggaacatcg cgacacctga ggccgttgtt cgggacgacg tcgccggacc
     3901 tgctccactc atgtaccgtt ggcgccacga catacacacc attcccggga ccgctccgcg
     3961 acatgggatg accctgaacg tcggggccgt cccatttgtg gtgccgcccc ggcatacgca
     4021 cgagggggtc acgccatggg gcgcggccga gccaaagcca agcagacgaa ggtcgctcgc
     4081 gagctgaagt acttcagccc cgacaccgac ctctccgcgc ttgagcgcga acttcacggt
     4141 gtttcccaga cgggcggcgg tcgcacctcg accgacgacc gcgaggaaga cgacgacgac
     4201 tacgggtcgt ggtctccggg ccggcgttga cgccaccctg accgcgccac ctcgtgcggc
     4261 ccgcaccctg cgtgcgggcc gtcgttgacc cctacctcag ccgggtgtcg gggtcgctga
     4321 tcggcgtgcc cgcgggccta tgctcgcccc atgactgaca ccaccgtcgc cgccacgcag
     4381 cggctgggga tcgagaccac gggcatcgag atcatcgacg agtccgagcg aacggcccag
     4441 cccaaagacc tgttctggcc gtggttcgcg gccaacgtgt cggtcttcgg catctcctac
     4501 gcggcgttcg tgctcgggtt cggcatctcg ttctggcagg ccaccctggt cacggtgctc
     4561 ggcatcgtca cctcgttcgt gctgtgcggc atcgtcgcga tcgccggcaa gcgcggttcg
     4621 gccccgacga tgatcctgtc tcgcgccgcc ttcggggtga ccgggcagaa ggtgccgggc
     4681 gtcatctcgt ggctggtgtc gatcggctgg gagaccttcc tggcgatcat ggccgtgctc
     4741 gccaccgcga ccgtgttcga gcagctcggt tggggcggcg gcacggggac gaaggtcgtg
     4801 gcagccgtcg tcgtcgcggc gatcatcgtg ctcgcgtcgg tggcggggta ccacatcatc
     4861 atgcggatgc agtcggtgct cacctggctc accggcgccg tgacgatcct cttcgtcatc
     4921 atcgcctctc gcgagatcga ctggtcagcc gtcgccgcgc tgccctccgg cccgacccag
     4981 tcggtgatcg gggcgttcgt catgctcatg acgggcttcg gcctgggctg gatcaacatc
     5041 tccgcggact ggtcgcgcta ccagcaccgg tcagcctccg gtggcgccat cgtcttctgg
     5101 aacaccctcg gcggtgcctt ggctcccgtc ctgctcgtga ccttcgggct catgctctcg
     5161 gcatccaacg aggaggtgct ggccggcatc cggagcgacc cgatcggcac cctcgcgacc
     5221 atcctgccga cgtggttcct cgtgccgttc ctcgtcgcgg tgatcatgtc gctgatctcc
     5281 ggtgccgtcc tcggcatcta ctcgtccggg ctgacgctgc tctcgctcgg ggtgcgcgtc
     5341 ccgcggccgg ccgcggccct ggtcgacggc atcatcctca ccctcggcac gttcttcgtc
     5401 gtcttcgtgg cgcaggactt cctcgggccg ttccagagct tcctcatcac cctcggggta
     5461 ccgctcgcgt cgtgggcggg gatcatgatc gccgacatcg ccctgcgcaa gcgcgactac
     5521 gacgacgacg ccctcttcga cgtgcgcggc cgctacggct cgtgggactg ggtgtcgatc
     5581 gcgacgatgg tggtcgcgtc ggtgatcggg tggggcctgg tcgtcaacaa cttcgcccag
     5641 gatgccgcgt ggaacaactg gcagggctac ctcctggagc cgctcggcct cggaacatac
     5701 atcaccgaag gcgaaggcgc cccctactgg gacggcgcct ggccgtacgc caacctcggg
     5761 gtgctgctcg ccctggtcct gtcgttcgcc gtgacctacg tggcccgccg ggcaagggtg
     5821 cagcagcagg aagcgctcgt cgatggctga cgcgctggct cgccctgacc acccgcagac
     5881 cttgggcgac gactggctcg tcatcatcga cccccaggtc gtcttcgccg acccgcaggc
     5941 ctcgccgtgg gggtcgtcga tgtgggccgg ggcggtcggt cacatcgcga cgctcgccgc
     6001 ggcatccacc ccgtcgcgca ccatcatcac gcgcttcgtg gccgatcccg gcctcggagg
     6061 gtcgtggggt ccgtactacg aggagtggcc gttcgcgctc gtgcctgacg gcgacccgct
     6121 gtacgccgtc gtgccggaac tggccggcgt tgccgagcac gtcgtcacgg cgggcacgtt
     6181 cggcaagtgg ccggtgctgc gcgacctcgt cggcgacggt gcccggctga ccctgacggg
     6241 ggtgtcgacc gactgctgcg tgctctcgac ggcgctgccg gcggcggatg ccggggccac
     6301 ggttcgcatc gcggccgccg ggtgcgcggg gtcgagcgag gagaaccacc ggcgggccct
     6361 ggatgccatg gccctcttcg ggccgcagat caccatcgtc gactgaaccg tcgtcgactg
     6421 aaccgtcgtc gactggacgg cccgcggggc acctgggtca gcggctgagg aagaacagcg
     6481 ttgctccggc cagcacgccc agggctccgg cgaggaggcc tgcggtgcgc gcccccaacc
     6541 acgtggcccg cacggcagtg gcggccgcgg cgaacccgag gcaccaggcg acgagcaggc
     6601 cgggcagcac ggcagccgtg aaaccggcga actcagtggc gaacgacccc ggcaggccgc
     6661 tgaggttcag ggcaccgaag atcgaccggc cgaccaccac ccgagccacg acgaggacga
     6721 ggactgcgcc ggtggccacg gtgagggccc cgcgagccca cgccatgacg acgcccgcac
     6781 gaccggcatc cgagatctgg cgtcggctcg tgaggggttc ctgctgacga gcggcggacg
     6841 accgacgccc gagagcggac gtccggtgca cggcggggtt cagatcggct gtgggcaccc
     6901 catgaccctc gccgcctcgg gctcagttgt catgagcccg ggtactcacc caccccgacg
     6961 tcgtggtggg ggtggctgca gtgcaagaag tcgcgggtca ggcggagctg gggtgggagc
     7021 cgacgacctg gaccgagccc ccgtcgacac ccttggcgcc gctgacgacc tcgacgccgt
     7081 cctcgatcgg ggcggtggcg gcctcgtgga cctcgcccat cgcccaggtc cggatgccgc
     7141 gttcctcgag gagcgctgtg gcggcatcga ccgagctggc cgggaccacg gccacgaagc
     7201 cgaccccttg gttcagggtg cgctcgaggt cggcctgcgg caccgagccg agtcggccga
     7261 cggtggagaa gatcgccggc ggggtccagg tggagcggtc gacccgggcg tgcaggtggt
     7321 cgggcaggac acgcgcgagg ttggccgcga gcccgccgcc ggtgacgtgc gagagggcgt
     7381 ggacgtcgac gccgtcggcc cggatgaggg cgagcaggtc ggcggagtag acgcgggtcg
     7441 gctcgaggag ctcttcgccc agggtgcgcc cgaactcgtc gacgtgccgg tcgagggccc
     7501 agccggcgtg ggcgatgacc cgacgcacga gggagtagcc gttggagtgc aggccgctgc
     7561 tggccaggcc gaggacgacg tcgccggggg cgacccgctg cgggccgagg acgtcggcgt
     7621 actcgacgac accggtggct gcgccggcca cgtcgtactc gtcgactccg agcagcccgg
     7681 ggtgctcggc ggtctcacca ccgaccaggg cgacgccggc ctgctcacac gcgcgggcga
     7741 tgccggacac gatggccgcg atgcgctcgg gcacgacctt gccgcacgcg atgtagtcgg
     7801 tcatgaacag cggctcggcg ccgcacacga cgatgtcgtc gacgaccatc ccgacgaggt
     7861 cgaacccgat ggtgtcgtgc cggtcgaggg cctgcgcgat ggcgaccttg gtgccgacgc
     7921 cgtcggtgct cgtggcgagc acggggcgtg ccatccgggc gatcgcgctg gcatcgaaca
     7981 ttcccgcgaa gcccccgagg cccccgagca cctcgggtcg ggtggcccgg cgcacgctgg
     8041 ccttcatcag ctcgacggcc ttgtcaccgg cctcgacgtc gacgcc
//
LOCUS       DAOSOC010000110         8077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_8893_length_8077_cov_9.747320, whole genome shotgun sequence.
ACCESSION   DAOSOC010000110 DAOSOC010000000
VERSION     DAOSOC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8893_length_8077_cov_9.747320"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PL014_08920"
     CDS             complement(<1..460)
                     /locus_tag="PL014_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT57252.1"
                     /translation="MTSTEIVAAGPGPQAGLDDHIYNRLLKERIIFLGSDVRDDNANA
                     ICAQMLLLAAEDPKKDIWLYINSPGGSVTAGMAIFDVMQWIPNDVATVAMGMAASMGQ
                     FLLSAGAPGKRYATPHARVMMHQPSGGIGGTASDIKIQAEQLLHIKKQMAE"
     gene            complement(699..1091)
                     /locus_tag="PL014_08925"
     CDS             complement(699..1091)
                     /locus_tag="PL014_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOT57253.1"
                     /translation="MDTNITSFWEILLWSFWIFIWISAVMIWFRCVFDMFSDKTLSGW
                     GKAGWAIVLVFLPWIGALIYLIARGKSMGERQVAAISAAQAQQEAYIQQVAAKSSPAD
                     QIASAKSLLDSGVISQAEFDALKAKALA"
     gene            complement(1258..2490)
                     /locus_tag="PL014_08930"
     CDS             complement(1258..2490)
                     /locus_tag="PL014_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT57254.1"
                     /translation="MPDAAPTTQPLDRSRVMTARNAVFVIFALAGLVFASWASRIADT
                     KEALGLSAGELGMTLFAASVGSVTGLPLAGRISQRIGATRAVALGTAVALSGLLVVGA
                     VVDAGGSRLALAAGLFLVGLGVGVWDVSMNLEGADVERGLGTAVMPHFHAAFSGGTVG
                     SALIGAGMSWAGVPLTIHFIGAAAVTVLLSWWALPKFLPHTAEHHAEAKAARERHGSA
                     WLEPRTLLIGVVVFAAAFTEGTANDWLAVAFVEGHDLPAWAGVLAFATFLTAMTIGRL
                     VGTRLLDRHGRVPVLRVSFALAALGAAMVIFGSAWLAFIGAAVWGVGAALGFPVGMSA
                     SADDATRAAARMSVVATIGYVAFIAGPPLLGLLGDSVGVLRSLLVVGALAILALSVVP
                     AVREPAGGTGDRAPAASA"
     gene            2647..3732
                     /locus_tag="PL014_08935"
     CDS             2647..3732
                     /locus_tag="PL014_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT57255.1"
                     /translation="MAPTRQTRSTQRRPTLRDVARRAGVSTSTASLVFSGKGPVAEAT
                     AQRVRDAASALSYAGPDPLASSLRQGRVGTVAVVVEGPLRYAFHDPFALAILDGLAEE
                     LDAAGRSMLLIAQPLDDPERAVAQLATQAVDAAVFPLCGHRDNPVVDHLLARGVPLVG
                     SGAPIHPRVAHVLTDEFAAMRLTTQHVLDLGHEQVAHVSMMLRAHSPTGVVTEAEVAA
                     ATYPDAVGRLAGFRSLAGPSAPVVQACELTVEAGEAAARLLLDVPAEQRPTAIVAQAD
                     LLAAGVVRAAESLGLRVPEDLTVTGFDGVDLPWLGHVLTTVDQPGAEKGRRMGRLVRL
                     ALEGRPVADETYPVTLRVGTTSAPPPA"
     gene            complement(3814..5229)
                     /gene="tig"
                     /locus_tag="PL014_08940"
     CDS             complement(3814..5229)
                     /gene="tig"
                     /locus_tag="PL014_08940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOT57256.1"
                     /translation="MKSAVETISPTRVKLSVEVPFAELKPMLDDAYRTIGAQVQIPGF
                     RKGKVPSRIIDQRVGRGAVVQEAVNEALPRFFAEAAEEQNVRAIGQPEVDVTAVPLED
                     GQDFTFTVETDVRPEVELPDLEGIAVEVDVVEATEDDITERLTALQERFGALVGVERA
                     VEDGDFVSIDLSATIGGEEIDAVTGVSYEVGSGNMLPGMDEALIGMSAGESKDFTAPL
                     AGGDREGEDAECTVTVQAVKVRELPALDDEFAQLASEFDTLEELRADVARQAEQSKKY
                     EQGIQARDKVLEHLLETVDVAVPEGIVEAEVHQHLEGENRLDDDEHRAEVQESTRRAL
                     KAQFLLDAIAEKLEVRVEQPELIEYLVMSAQQYGMDPNTFAQTIDQQGQIPSIVQEVA
                     RRKGLAAVLDQAVVTDTAGTVIDLDELVPQSDELETFEVDEVVETEEIIEVVELVETD
                     EADADAEVTESADDAEATDKA"
     assembly_gap    5506..5605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5606..5876
                     /locus_tag="PL014_08945"
     CDS             <5606..5876
                     /locus_tag="PL014_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOT57257.1"
                     /translation="THEVGHWMMLEHTFAHGCGASGDYVADTPKEAGPQFDCPDGADT
                     CKAPGLDPIRNFMDYTQDECMNEFTPGQVERMSDAWQQFRAGAKA"
     gene            complement(5959..6627)
                     /locus_tag="PL014_08950"
     CDS             complement(5959..6627)
                     /locus_tag="PL014_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT57258.1"
                     /translation="MARKIAQSGGVVVIGLGRFGKSLADQLERDDVQVLAIDNDPKRV
                     QDLAGRWTHVVQADSTDIQAMEQLGVGEFSKAVIGIGHNLEASVLTAFVLRKLGVSDI
                     WAKAITTSQGEILEQVGVSHVVRPEHAMGKRVAHLVRGRMLDYIEFDDGYAIVKTTPP
                     HGILGKTLADAGVRSRYGVTIVGIKSPGQEFSYATPTSVVNRGDLIIVSGDRRKVELF
                     SDQE"
     gene            complement(6620..7990)
                     /locus_tag="PL014_08955"
     CDS             complement(6620..7990)
                     /locus_tag="PL014_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOT57259.1"
                     /translation="MFSSRSGRPARRRPVFRHPAQVVVTAFLVADIVGTLLLLLPVSR
                     ADVGSAPFITALFTATSAVCVTGLATVDTATYWSTTGQAIILALIQVGGFGIMTLASL
                     IALFLSRRMGLRTRLTAAAETKAIGPGDVRRVLRGVFLITVVVEATIAVMLTLRFRAE
                     YDGSWATATWHGVFHAVSAFNNAGFALFSDNFMGFLTDPFVLLPITLAIIIGGLGFPV
                     LVELLRSRRSRSTRTWSLHTRLTLLMTGALLVGGTLFITASEWSNPGTLGPLGPADKL
                     LGGWFHAVQPRTAGFNAWDYGEITDETLLGTILLMFVGGGSAGTAGGLKVTTFIVLFF
                     VILAEVRGDRDVIAFDRRIDHRVVRQATTVALLGVAAVVGGTMLLTELTELPLQDVMF
                     ETTSAFATVGLSTGITPQIGGAGQLVLVFLMFLGRLGPITLVSALALRENTRRYTLPE
                     GAPLIG"
BASE COUNT         1242 a         2736 c         2838 g         1161 t
ORIGIN      
        1 gctcggccat ctgcttcttg atgtggagca gctgctcggc ctggatcttg atgtccgagg
       61 ccgtaccgcc gatgccacca ctgggctggt gcatcatgac gcgggcgtgc ggggtggcgt
      121 agcgcttgcc cggtgcgccg gcggagagca ggaactgccc catcgaggcg gccatgccca
      181 tcgcgaccgt cgcgacgtcg ttggggatcc actgcatgac gtcgaagatc gccatgccgg
      241 cggtgacgga cccgcccggg gagttgatgt agagccagat gtccttcttg gggtcctcgg
      301 cggcgagcag gagcatctgg gcgcagatcg cgttcgcgtt gtcgtcgcgc acgtcggacc
      361 cgaggaagat gatccgctcc ttgaggagac ggttgtagat gtggtcgtcc agcccggcct
      421 gggggccagg gcctgcggcg acgatctcgg tgctggtcac gctggagtcg ctcctcgtca
      481 gatcggggtg ctgctgtgtc agtgacccta acgccggacc tcggtacgac actccccggt
      541 ccgggctgtg ttcgccgtga gcacaacgcc gccgcggacc actcaggctt tgccacagca
      601 cctccccgac gtttcgtgcg aggagcccgg caccacgccg ctgacgcggt gtggtgccgg
      661 gctccgaaca ggaagggtcg cgccccccgg gggtgagctc aggcgagggc cttggccttc
      721 agggcgtcga actcggcctg gctgatcaca ccggagtcga gcagcgactt ggcgctggcg
      781 atctggtcgg ccggcgagct cttcgcggcg acctgctgga tgtacgcctc ctgctgcgcc
      841 tgcgcggccg agatggccgc cacctggcgc tcacccatgc tcttgccgcg ggcgatgagg
      901 tagatcaggg cgccgatcca gggcaggaag accaggacga tcgcccagcc ggccttgccc
      961 cagccgctca gggtcttgtc gctgaacatg tcgaagacgc agcggaacca gatcatgacc
     1021 gcggagatcc agatgaagat ccagaagctc cagaggagga tctcccagaa gctggtgatg
     1081 ttggtgtcca tggacggaaa tgcctttcgc agagtgaggg gtgtgctctc gcgagccaca
     1141 accgggtcgc gggacccggc gggccagtgt ttcattgttg attggaaacc acgtcacgac
     1201 atcccccgaa ccgggtgaga gtcggtatcc tcacccactc ccgagcgcga ggacccctca
     1261 ggccgaggct gcgggcgccc ggtccccggt ccctccagcg ggctcgcgca ccgcgggcac
     1321 gaccgacagc gcgaggatcg cgagggcacc gacgacgagc agcgagcgca gcacaccgac
     1381 gctgtccccg agcaggccca gcaacggcgg gccggcgatg aaggcgacgt agccgatggt
     1441 cgcgacgacc gacatccggg ccgctgcccg agtggcgtcg tcggccgagg cgctcatccc
     1501 gacggggaag ccgagggccg cgccgacacc ccagacggcc gcaccgatga aggccagcca
     1561 ggcgctgccg aagatcacca tggcggcacc gagggccgcc agggcgaagc tcacgcgcag
     1621 caccggcacc cgaccgtggc ggtcgagcag gcgggtgccg acgaggcgcc cgatggtcat
     1681 cgccgtcagg aacgtcgcga acgccagcac cccggcccag gccggcaggt cgtgcccctc
     1741 gacgaaggcg acggcgagcc agtcgttcgc ggtgccctcg gtgaacgccg ccgcgaagac
     1801 gacgaccccg atgagcaggg tgcgcggctc gagccacgcc gagccgtggc gctcacgggc
     1861 tgccttggcc tccgcgtggt gctcggcggt gtgcggcagg aacttcggga gcgcccacca
     1921 cgacaggagc acggtgaccg cggcggcacc gatgaagtgg atcgtcagcg gcacacctgc
     1981 ccacgacatc cccgcaccga tgagtgccga ccccacggtt ccgccgctga aggccgcgtg
     2041 gaagtgcggc atgacggcag taccgagacc acgctcgacg tcggccccct cgaggttcat
     2101 cgacacgtcc cagacgccga cgcccagccc gacgaggaac agccccgccg cgagcgccag
     2161 ccgcgagccc ccggcatcca cgaccgcgcc gacgacgagc agcccggaga gggcgacggc
     2221 cgtgccgagt gcgacggccc gggtcgcccc gatccgctgc gagatccgac cagccagcgg
     2281 cagtcccgtc accgacccca cgctcgcggc gaacagggtc atgccgagct cgcccgcgct
     2341 cagcccgagc gcctccttgg tgtcagcgat gcgcgaggcc cacgaggcga agaccagacc
     2401 ggccagggcg aagatcacga agacggcgtt gcgtgccgtc atgacgcggg accggtcgag
     2461 gggctgcgtg gtgggggcgg cgtcaggcat ggcggacaac gggctttcct tcgattctct
     2521 ggtggtgaag tgtggcgcac ggcacgtgag gtgcgcaggt ggctggtggg ccgaccacgg
     2581 gtgcgatcgc accctgaagg cgggatcgaa tcgattcgat cgaatcgatt cgatattgtg
     2641 tctcccatgg cccccactcg tcaaacgcga tccacgcagc gccgcccgac gctgcgtgac
     2701 gtcgcgcgcc gcgccggggt gtcgacgtcg acggcatccc tggtgttcag cgggaagggg
     2761 ccggtcgcgg aggcgaccgc ccagcgggtg cgcgacgcgg catccgcgct gtcgtatgcc
     2821 gggcccgacc ccctggcgtc gtcgttgcgc cagggccggg tcggcaccgt cgccgtcgtc
     2881 gtcgaggggc cgctgcggta cgccttccac gaccccttcg ccctggcgat cctcgacggc
     2941 ctcgcggagg aactcgatgc agccggtcgg tcgatgctgc tcatcgccca gccgctcgat
     3001 gaccccgagc gggccgtggc ccagctcgcg acccaggccg tcgacgcggc tgtcttcccg
     3061 ctgtgcggcc accgcgacaa ccccgtcgtc gaccacctgc tggctcgtgg ggttccgctg
     3121 gtcggcagcg gcgccccgat ccacccgcgc gtcgcccacg tcctcaccga cgagttcgcc
     3181 gcgatgcgcc tcacgaccca gcacgtgctc gacctcggcc acgaacaggt cgcgcacgtg
     3241 agcatgatgc tgcgggccca ctcccccacc ggcgtggtca ccgaggccga ggtcgccgcc
     3301 gccacctacc cggatgccgt gggtcgactc gccgggttcc gctcgctcgc gggcccctcg
     3361 gctcccgtgg tgcaggcctg cgagctcacc gtggaggcgg gcgaggcggc tgcccgcctc
     3421 ctgctcgacg tccccgcgga gcagcgcccg acggcgatcg tcgcccaggc cgacctgctg
     3481 gcggcaggtg tcgtgcgcgc tgccgagtcg ctcggcctgc gagtgcccga ggacctcacg
     3541 gtcaccgggt tcgacggcgt cgacctgccg tggctcgggc acgtgctcac gaccgtcgac
     3601 cagcccggtg ccgagaaggg ccgccggatg ggacgactgg tgcgactggc gctcgagggc
     3661 cgcccggtgg ccgacgagac ctacccggtg accctgcggg tcggaacgac gagcgcccca
     3721 cccccggcat gactcagccg gtgtgcgtga agaccgtcgg gatgaggacg ttcttcacac
     3781 acaccggctg aggtcggtgc tgcgtgctgg cgatcaggcc ttgtcggtgg cctctgcgtc
     3841 gtcagccgac tcggtgacct cagcgtcagc gtcggcctcg tccgtctcga ccagctcgac
     3901 gacctcgatg atctcctcgg tctcgacgac ctcgtcgacc tcgaacgtct cgagctcgtc
     3961 ggactgcggc acgagctcgt cgaggtcgat cacggtgccg gccgtgtcgg tgacgacagc
     4021 ctggtcgagc acggcggcca gacccttgcg gcgggcgacc tcctgcacga tcgaggggat
     4081 ctgaccctgc tggtcgatcg tctgcgcgaa ggtgttcggg tccatgccgt actgctgggc
     4141 gctcatgacg aggtactcga tgagctcggg ctgctcgacg cggacctcga gcttctcggc
     4201 gatggcgtcg agcaggaact gggccttgag cgcccggcgg gtgctctcct gcacctcggc
     4261 acggtgctcg tcgtcgtcga ggcggttctc gccctcgagg tgctggtgga cctcggcctc
     4321 gacgatgccc tcgggcacgg cgacgtcgac ggtctcgagc aggtgctcga gcaccttgtc
     4381 acgggcctgg atgccctgct cgtacttctt gctctgctcg gcctggcgcg cgacgtcggc
     4441 gcgcagctcc tcgagggtgt cgaactcgga ggcgagctgg gcgaactcgt cgtcgagcgc
     4501 gggcagctca cggaccttga cggcctgcac ggtgacggtg cactcggcgt cctcgccctc
     4561 gcggtcgcca ccggccagcg gcgcggtgaa gtccttggac tcgccggcgc tcatgccgat
     4621 gagggcctcg tccatgccgg gcagcatgtt gccgctgccg acctcgtagg agacaccggt
     4681 gacggcgtcg atctcctcgc cgccgatcgt cgccgacagg tcgatggaga cgaagtcgcc
     4741 gtcctcgacg gcccgctcga caccgacgag ggcgccgaag cgctcctgaa gggcggtgag
     4801 gcgctcggtg atgtcgtcct cggtcgcctc gacgacgtcg acctcgaccg cgatcccctc
     4861 gagatcgggg agctcgacct cggggcgcac gtcggtctcg acggtgaagg tgaagtcctg
     4921 gccgtcctcg aggggcacag cggtgacgtc gacctcgggc tggccgatgg cgcgcacgtt
     4981 ctgctcctcg gcggcctcgg cgaagaagcg cggcagcgcc tcgttgacgg cctcctggac
     5041 cacggcgccg cgcccgacgc gctggtcgat gatgcggctc gggaccttgc ccttacggaa
     5101 gccggggatc tgcacctgtg caccgatggt gcggtaggcg tcgtcgagca tcggcttgag
     5161 ctccgcgaac gggacctcca cgctgagctt gacccgggtc gggctgatgg tctcgacggc
     5221 actcttcact tcaggcactc cacaaccatg ggggaatcgg gggacaaatc gcttggatgc
     5281 cgtacggccg gccgcgagcg gaccggcacc tcccagcggc gcgcgagcgc gcggcatccg
     5341 ggtagggcac caagggccca actgtagcgg ccaccaacag ggccacccaa tccgccgtcg
     5401 agatcggcct ctcacgacgt tgcggtgggt accctcccga acagcgcggc ccggaacccg
     5461 ggcagcccga cccgagaagg accccgatga ccctcacttc caccannnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnncaccc acgaggtcgg ccactggatg atgctcgagc
     5641 acaccttcgc gcacggctgt ggggcctccg gcgactacgt ggccgacacc ccgaaggagg
     5701 ccgggccgca gttcgactgc cccgatggcg cggacacctg caaggccccg ggcctggacc
     5761 cgatccgcaa cttcatggac tacacgcagg acgagtgcat gaacgagttc acgccgggtc
     5821 aggtcgagcg gatgagcgac gcctggcagc agttccgggc gggcgccaag gcctgatcca
     5881 cgcagcaccg ctggttgcgt ccggcgttct ggaggtcctg acagccagaa cgccggacgt
     5941 tgctggttga aggaccagtc actcctggtc ggagaagagc tcgaccttgc gccggtcccc
     6001 cgagacgatg atcaggtcgc cccggttcac caccgaggtc ggcgtggcgt acgagaactc
     6061 ctggcccggt gacttgatgc ccacgatggt gacgccgtag cgcgagcgca ccccggcatc
     6121 cgccaaggtc tttccgagga tgccgtgggg cggggtcgtc ttgacgatcg cgtagccgtc
     6181 gtcgaactcg atgtagtcga gcatgcgtcc gcgcacgagg tgggccaccc gcttgcccat
     6241 cgcgtgctcg ggacgcacga cgtgcgagac ccccacctgt tcgaggatct cgccctgtga
     6301 ggtcgtgatg gccttggccc agatgtcgct gacgccgagc ttgcgcagca cgaacgccgt
     6361 gagcacgctc gcctcgaggt tgtggccgat cccgatgacc gccttggaga actcccccac
     6421 gccgagctgc tccatggcct ggatgtcggt ggagtctgcc tggacgacat gggtccagcg
     6481 gccggcgagg tcctgcaccc gcttggggtc gttgtcgata gcgagcacct gcacgtcgtc
     6541 gcgctcgagc tggtcggcga gtgacttgcc gaaccggccg agaccgatca cgacgacgcc
     6601 accggactgg gcgatcttcc tagccaatga ggggcgctcc ttcgggcagg gtgtagcggc
     6661 gggtgttctc acgcagggcg agggccgaca ccagggtgat cgggccgagc cggcccagga
     6721 acatgaggaa gacgagcacg agctgacccg ccccaccgat ctgcggggtg attccggtcg
     6781 agagccccac cgtcgcgaag gccgaggtcg tctcgaacat cacgtcctgc aacggcagtt
     6841 cggtcagttc ggtcagcagc atcgtgccac cgacgaccgc ggccactccg agcagcgcca
     6901 ccgtggtcgc ctggcgcacg acccggtggt cgatgcgccg gtcgaaggcg atgacgtcac
     6961 ggtcgccgcg tacctcggcg aggatcacga agaacaggac gatgaaggtc gtcaccttca
     7021 ggcctccggc ggtgccggcc gagccgccgc cgacgaacat cagcaggatg gtgccgagca
     7081 gggtctcgtc ggtgatctcg ccgtagtccc acgcgttgaa gccggcggtg cgcggctgga
     7141 cggcgtgaaa ccacccgccg agcagcttgt cggccgggcc gagcgggccg agggtgccgg
     7201 gattgctcca ctcggaggcg gtgatgaaga gcgtgccgcc gacgagcagt gcgccggtca
     7261 tgagcagggt gaggcgggtg tgcaggctcc aggtgcgggt gctccggctg cgccgcgagc
     7321 gcagcagttc gacgagcacc gggaagccga gcccgccgat gatgatggcg agggtgatcg
     7381 gcagcagcac gaacgggtcg gtgaggaagc ccatgaagtt gtcgctgaac agcgcgaagc
     7441 cggcgttgtt gaaggccgag acggcgtgga agacgccgtg ccaggtggcc gtggcccagg
     7501 agccgtcgta ctcggcccgg aagcgcaggg tcagcatcac ggcgatcgtg gcctcgacga
     7561 cgacggtgat gaggaagacc ccgcgcagca cccgccgcac gtcaccgggg ccgatcgcct
     7621 tggtctcggc cgcggccgtc agccgggtgc gcaggcccat ccggcgcgag aggaacaggg
     7681 cgatgagaga ggccagggtc atgatcccga agccaccgac ctggatcagc gcgaggatga
     7741 tcgcctgccc ggtcgtgctc cagtaggtcg ccgtgtcgac cgtggccagc cccgtgacgc
     7801 agaccgcgct cgtggccgtg aacagtgccg tgatgaacgg ggcgctgccc acgtcggccc
     7861 gggagacggg gagcaggagc agcagggtgc cgacgatgtc cgcgacgagg aaggcggtga
     7921 ccaccacctg cgccggatgc cggaacaccg ggcgacgacg ggccgggcgt cccgacctcg
     7981 aggagaacac ccgctcagga tggcacgctg gtggagcatg tgtggagcgg gcgacgagaa
     8041 tcgaactcgc gtagccagtt tggaagactg gggctct
//
LOCUS       DAOSOC010000111         7991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9020_length_7991_cov_9.329385, whole genome shotgun sequence.
ACCESSION   DAOSOC010000111 DAOSOC010000000
VERSION     DAOSOC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9020_length_7991_cov_9.329385"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PL014_08960"
     CDS             <1..846
                     /locus_tag="PL014_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOT57260.1"
                     /translation="RLHRALAGRVVQRAELRWPRVPDVDLAGAHTLEVVARGKHLLHR
                     FDTGTTLHTHLRMEGQWRIEHPGAATQRALRRSDLRAAVLTDEWSAAGLRLGMLDLVP
                     TSREGDLVGHLGPDVLGADWDPELVAARLTASEAFVGDLLLDQRVLAGVGTFWASEIL
                     FLERVLPWTPGRDLGDAVAARITDRLHRLMDLARRTGWQASTGVQRSGEEAYVHARSG
                     RPCRRCGDTVRVAMTGLAPRARTLFSCPTCQGGLAPTDEGRPQRPLGSGGSGSGRTGS
                     GRSGR"
     gene            complement(835..1143)
                     /locus_tag="PL014_08965"
     CDS             complement(835..1143)
                     /locus_tag="PL014_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT57261.1"
                     /translation="MILLRHELGEVLREIRQDQGRTLRDVASTSAVSLGYLSELERGT
                     KEASSELLAAVCAGLGLNLSEVLELVRDRVERVETFSTPVSLPVSATVDPDQVSASAA
                     "
     gene            complement(1140..1697)
                     /locus_tag="PL014_08970"
     CDS             complement(1140..1697)
                     /locus_tag="PL014_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005507108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOT57262.1"
                     /translation="MSDAGAPAVVAGLRERGLTVATAESLTGGLVCAALTSVPGASTV
                     VRGGVVAYASEVKASVVGVDAGVLAREGAVSAAVAGELATRVRAVMGCDVGVSTTGVA
                     GPDPADGQPPGTVFIGACGPWGILVEELSLTGNREQIRAATVRAALTLLTTALQNLRD
                     ADGPAGYGGARSEQPVDTNEREDAP"
     gene            complement(1742..2374)
                     /gene="pgsA"
                     /locus_tag="PL014_08975"
     CDS             complement(1742..2374)
                     /gene="pgsA"
                     /locus_tag="PL014_08975"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOT57263.1"
                     /translation="MPTTGPSAWNIANYLTVFRLAIVPVFGWLLLADDGRSAVYRWAA
                     FAVFVVAMVTDRIDGDLARSRGLVTNVGKVLDPIADKALVTMALIGLSAVGEVWWWVT
                     IVVLVREWGITAVRFWVIRHGVMAAGRGGKVKTLLQTFGIGFYLMPRWVLPYPDVWEA
                     LAAISLGAAVLITVVTGVDYLHQALRLRQTSERAASKRAARAARRGGRGA"
     gene            complement(2515..3936)
                     /gene="rimO"
                     /locus_tag="PL014_08980"
     CDS             complement(2515..3936)
                     /gene="rimO"
                     /locus_tag="PL014_08980"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOT57264.1"
                     /translation="MPSTPSRGSVALVTLGCTRNEVDSEELAGRLSAEGWTLVDDAAD
                     ADVALVNTCGFVEQAKKDSIDTLLEAADLKESGRTKAVVAVGCLAERYGQTLADELPD
                     ADAVLGFDSYRDMSSHLTAILAGERPASHVPSDRRRLLPLSPVDRSAAADAVALPGHG
                     DGEAFVRSRLGGGPWAPLKIASGCDRRCSFCAIPMFRGSFVSRRPTDVLAEARWLGEQ
                     GVSELFLVSENSTSYGKDLGDLGLLEKLLPELTAVPGVERVRVSYLQPAEVRPGLLAA
                     MADTPGVMPWYDLSFQHSSETVLRRMRRFGGTEPFLDLIGRVRDRQPLAGVRSNVIVG
                     FPGETGADVDELERFLTQARMDVVGVFGYSDEDGTEAESLGDKLPEHVVLERVERITG
                     LVEELTAQRASERVGERVIVLVEDHDEDDEGVAIGRAEHQGPDVDGVCLVRAADGALP
                     AVGQFVEGEVVDSVGVDLVVEVR"
     gene            3993..5102
                     /locus_tag="PL014_08985"
     CDS             3993..5102
                     /locus_tag="PL014_08985"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOT57265.1"
                     /translation="MASETDARVHDLLRHHPVIDGHNDLPWEARERVAYDWAELDIAL
                     PGQPTHTDIPRLRAGGVGAQFWSVYVPSDLPGSQAVTATLEQIDAVHAMIARYPDDLV
                     AARTASDVERAISQGRIASLIGAEGGQSIDSSLGALRMLFSLGVRYLTLTHNDNVPWA
                     DSATDDPVLGGLSAFGREVVLEMNRLGMLVDLSHVSADTMRAALDTTEAPVIFSHSSA
                     RALCDHPRNVPDDVLARLADNGGVCMVTFVPYFVSQALRDWDREPTDAPRPQASIDDV
                     VAHCEHVREVAGIEHIGLGGDYDGVDHLPTGLEDVSGYPRLLAALAERGWSDTDLAAL
                     TSGNILRVLREAEARAAELSTARGPSLARIEDLDG"
     gene            complement(5126..5827)
                     /locus_tag="PL014_08990"
     CDS             complement(5126..5827)
                     /locus_tag="PL014_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOT57266.1"
                     /translation="MPLMWEDLQEAPSRSDRWKATLRKQPSAFLLAVQILVILLLPVL
                     ESSSWGAAVISLLSLSALITAVFTVRSTPALTWLSATLALPAAVFEIWSLIDDSSTVV
                     ALAHSALAVFYFYTAYALISYMFEDSWVTKDELFAVGACFTVLVFAFAYLFMAIQEVY
                     PQSFTSFDGPGKRTLLELIYYSGANLTSVGLSDVGPIRPHARAIGTVEQLAGVMYVAM
                     VISRLVALTVMRARS"
     gene            5865..6869
                     /locus_tag="PL014_08995"
     CDS             5865..6869
                     /locus_tag="PL014_08995"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOT57267.1"
                     /translation="MPSALLLPVLTCAVVLLVSGVAKLRDPDAVDTAFVDLDVPAGLR
                     HPLLRRALPWVEIALGVWLVLATGSALVVVAALTLGLFLAYLWLVVRAVRMPEAVDCG
                     CFGALGDTRVTPVTVWRNIALVASAALSLALGLSGSGVLAALLEDPSLWGWLATAALA
                     AAVTGLVLYRPTADPVTAVDGVVTGELPVDDDGDYLPTPAPVAQLLDEAGALVLLSRE
                     TPAAAHLLIFLNPGCGSCEAVKPFVAQWSQDLAPVTVRAVLMVAPAALTSTWPDLSGL
                     AWFDPNGVARRAFGCATPGAVLIGTDGNLAGGPVNGAQAVTEFVDEIAEQLRAAHVTG
                     "
     gene            6906..7253
                     /locus_tag="PL014_09000"
     CDS             6906..7253
                     /locus_tag="PL014_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57268.1"
                     /translation="MPSDAQSTRGVTVLLRPSCGCFGRIGDQRVTARTVAAVVIAALA
                     VFILGNAAQTPKVARPAAGAAGWARDLGPVELTVVVPSTPAELARLPARRDRCLREGM
                     PRARTAARLTPPV"
     gene            complement(7398..>7991)
                     /locus_tag="PL014_09005"
     CDS             complement(7398..>7991)
                     /locus_tag="PL014_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOT57269.1"
                     /translation="DALFLPMGASKPMRVQGAWVTESEIHDVVKHVTDQLKPRYRDDV
                     TVTAPKKQVEDDIGDDLDLLLQATELVVTTQFGSTSMLQRKLRVGFAKAGRLMDLLES
                     RGVVGPSEGSKARDVLVKPDDLPTTLALMRGDDVPETFDAAPAAVTDTSTDDTSEVPV
                     VGYGGDAVAAELDSYAAAPEWNDEDGDQDAWELTGRR"
BASE COUNT         1131 a         2941 c         2814 g         1105 t
ORIGIN      
        1 cgcctgcacc gggcgctcgc cggtcgggtc gtgcagcggg ccgagctgcg ctggccccgg
       61 gtgcccgacg tcgacctcgc cggcgcccac accctcgagg tcgtggcccg cggcaagcac
      121 ctgctgcacc gcttcgacac cggcacgacc ctgcacacgc acctgcggat ggagggtcag
      181 tggcgcatcg agcacccggg ggcggccacg cagcgggcgc tgcgccggtc cgacctgcgg
      241 gccgccgtcc tcaccgacga gtggtcggcc gcggggctgc gcctcgggat gctcgacctc
      301 gtccccacca gccgcgaagg tgacctcgtc gggcacctgg gcccggacgt gctcggcgca
      361 gactgggatc ccgaactcgt cgctgcgcgg ctcacggcat ccgaggcctt cgtcggcgac
      421 ctcctgctcg accagcgcgt cctcgccggg gtcggcacgt tctgggccag cgagatcctc
      481 ttcctggagc gggtgctgcc gtggacgccg ggccgcgacc tcggcgacgc cgtggccgcg
      541 aggatcaccg accggttgca ccggctgatg gacctcgcgc ggcgcaccgg gtggcaggcc
      601 agcaccgggg tccagcgctc tggcgaggag gcctacgtgc acgcccggtc cgggaggccc
      661 tgccggcggt gtggtgacac ggtgcgggtg gcgatgacgg ggctggcccc tcgagcgcgc
      721 acgctgttct cgtgccccac ctgtcagggc gggctggcac cgaccgacga gggtcgcccg
      781 cagcgcccac tcggtagtgg tggctcgggc agtggtcgaa caggaagtgg ccgctcaggc
      841 cgctgacgcg gaaacctggt cggggtcaac cgtcgctgag accggcagcg agacgggggt
      901 ggagaacgtc tcgacccgct cgacccggtc acggaccagc tcgagcacct cggacaggtt
      961 caggcccagg cctgcgcaca cggccgcgag cagctcggag gacgcttcct tggtgccccg
     1021 ctcgagttcg ctgaggtagc ccaaggacac tgctgacgtc gacgcgacgt cgcgcagggt
     1081 gcggccctgg tcctgacgga tctctcgaag gacttcgccg agctcgtggc gcaacagaat
     1141 cacggcgcgt cctccctctc gttcgtgtcg accggctgtt ccgatcgggc tccaccgtac
     1201 cccgcgggac cgtcagcgtc tcgaaggttc tgcagtgccg tggtcagcag ggtgagagct
     1261 gcccgcacgg tggccgcgcg gatctgctcc cggttgccgg tcaacgacag ttcctccacg
     1321 aggattcccc acggcccgca ggcaccgatg aacaccgtcc ccggtggctg tccgtcggcc
     1381 gggtcggggc cggccacgcc ggtcgtcgac accccgacgt cacagcccat gacggcccgt
     1441 actcgcgtcg cgagctcacc ggctaccgct gcggacaccg ccccctcacg ggccagcacc
     1501 cccgcgtcga cacccaccac ggaggccttg acctcgctgg cgtaggcgac gacgccgccg
     1561 cgcacgacgg tcgacgcccc ggggacgctc gtcagcgcgg cacagaccag tcccccggtg
     1621 agcgactcag ccgtcgcgac ggtgagcccg cgctcacgca agccggcgac gacagcgggc
     1681 gcaccggcat ccgacacccc ggcatccgac accccggcat ccggccgctc gggcaccgtg
     1741 ctcaagcgcc acgaccccca cggcgggccg cgcgggccgc gcgcttgctc gccgcgcgct
     1801 cactggtctg gcgcaaccgc aacgcctggt gcaggtagtc gacaccggtg acgaccgtga
     1861 tgagcactgc cgcaccgagc gagatcgccg ccagcgcctc ccagacgtcg gggtagggca
     1921 gcacccaccg cggcatgagg tagaaaccga tgccgaacgt ctggagcagg gtcttgacct
     1981 tgcccccgcg gccagcggcc atgaccccgt ggcggatcac ccagaagcgc actgcggtga
     2041 tcccccactc gcgcacgagc accacgatgg tgacccacca ccacacctcg ccgaccgcgg
     2101 acagcccgat gagggccatc gtgaccagcg ccttgtccgc gatggggtcg agcaccttcc
     2161 ccacgttggt caccaggccg cggctgcgcg cgaggtcgcc gtcgatccgg tcggtgacca
     2221 tcgcgacgac gaagaccgcg aaggctgccc agcggtagac cgcactgcgc ccgtcgtcgg
     2281 cgagcagcag ccagccgaag accgggacga tggccagccg gaagaccgtc aggtagttgg
     2341 cgatgttcca cgcgctcggg ccggtcgtcg gcaccgcggg gctcgccggg tcgaccccga
     2401 tccgataggg gtcggtggcg cggccctgcg cgagcgggcc gggacgcact gctcgcagcg
     2461 gtttgtcccc cagtgccttg tcacccagtg gctgttcccc cgtcggcggc tcgctcatcg
     2521 cacctccacg acgaggtcga cccccaccga gtcgacgacc tcaccctcga cgaactgacc
     2581 gaccgctggc agggcgccgt ccgcggcccg cacgaggcac accccgtcga cgtccggccc
     2641 ctggtgctcg gcgcggccga tcgccacccc ctcgtcgtcc tcgtcgtggt cctcgaccag
     2701 gacgatcacc cgttccccca cccgctcgct cgcgcgctgt gccgtgagct cctcgacgag
     2761 gccggtgatc cgctcgaccc gctccaggac cacgtgctcg gggagcttgt cgccgagcga
     2821 ctcggcctcg gtgccgtcct cgtcggagta gccgaagaca ccgacgacgt ccatgcgagc
     2881 ctgcgtgagg aagcgttcga gctcgtcgac gtcggccccg gtctcgcccg ggaagcccac
     2941 gatcacgttg ctgcggaccc cggcgagggg ctggcggtcg cgcacgcgcc cgatgaggtc
     3001 gaggaagggc tcggtgcccc cgaaccggcg catccggcgc agcaccgtct cggaggagtg
     3061 ctggaaggac aggtcgtacc acggcatgac accgggggtg tctgccatcg ccgcgagcag
     3121 ccccgggcgc acctcggccg gttggaggta ggacacccgc acccgctcga cacccggcac
     3181 cgcggtcagc tcggggagca gcttctcgag caggccgagg tcacccaggt ccttgccgta
     3241 ggacgtcgag ttctcgctga cgaggaacag ctcgctcacg ccctgctccc cgagccaccg
     3301 ggcctcggcg aggacgtcgg tgggacgacg ggacacgaag gagccgcgga acatcgggat
     3361 ggcgcagaac gagcagcgac ggtcgcagcc gctcgcgatc ttcagcggtg cccacggccc
     3421 gcccccgagc cgcgagcgca cgaaggcctc accgtccccg tgaccgggca acgccacggc
     3481 atccgccgcg gcggagcggt cgaccggaga cagcggcagc agcctgcggc ggtcggaggg
     3541 gacgtgggat gccggtcgct cgccggccag gatcgccgtc aggtggctcg acatgtcgcg
     3601 gtaggagtcg aagccgagca cggcatcggc gtcgggcagc tcgtcggcga gggtctggcc
     3661 gtagcgctcc gcgaggcagc ccaccgcgac gaccgccttg gtgcgccccg actccttgag
     3721 gtcggccgcc tccaggaggg tgtcgatgga gtccttcttg gcctgctcga cgaagccgca
     3781 ggtgttgacc agggcgacgt cggcgtccgc cgcgtcgtcg accagggtcc agccctcggc
     3841 cgacaagcgg ccggccaact cctcggagtc gacctcgttg cgggtgcacc cgagggtgac
     3901 cagggcgacg ctgccgcgac tcggggtgga gggcatgacg ccactgtagt tcccggcagg
     3961 ggcgctccgg cacgtcgcag tagggtccgg gcatggcttc cgagaccgac gcccgcgttc
     4021 acgacctgct ccggcaccac cccgtgatcg acgggcacaa cgacctgccg tgggaggccc
     4081 gggagcgggt cgcctacgac tgggccgagc tcgacatcgc gctgcccggg cagccgaccc
     4141 acaccgacat cccccggttg cgagccggcg gcgtgggcgc ccagttctgg tcggtgtacg
     4201 tgccctccga cctacccggc agccaggcgg tgacggcgac gctcgagcag atcgacgcgg
     4261 tgcacgccat gatcgcgcgc taccccgacg acctcgtcgc cgcccgcacg gcatccgacg
     4321 tcgagcgggc gatctcacag gggaggatcg cctcgctcat cggcgccgag ggtgggcaga
     4381 gcatcgacag ctccctgggg gcgctgcgga tgctgttctc cctcggggtg cgctacctga
     4441 ccctgaccca caacgacaac gtgccgtggg cggactccgc caccgacgac cccgtgctcg
     4501 gcgggctgtc ggcgttcggc cgcgaggtcg tgctcgagat gaaccgcctc ggcatgctcg
     4561 tcgacctctc ccacgtgtcg gccgacacca tgcgggccgc cctggacacc accgaggcgc
     4621 cggtgatctt ctcgcactcc tccgcccggg cgctgtgcga ccacccccgc aacgtgcccg
     4681 acgacgtgct ggcccgcctg gccgacaacg gcggggtgtg catggtgacc ttcgtgccgt
     4741 acttcgtcag ccaggcgctg cgcgactggg accgcgagcc caccgacgcg ccccgtccgc
     4801 aggcgagcat cgacgacgtc gtcgcgcact gcgagcacgt gcgggaggtc gccggcatcg
     4861 agcacatcgg cctcggcggc gactacgacg gcgtcgacca cctccccacc ggcctcgagg
     4921 acgtcagcgg gtacccccgc ctgctcgccg cgctcgcgga gcgtggctgg agcgacaccg
     4981 acctcgccgc cctgacgagc gggaacatcc tgcgggtgct gcgcgaggcc gaggcgcggg
     5041 ctgctgagct ctccaccgcg cgcggcccca gcctggcccg catcgaggac ctcgacggct
     5101 gaacgacgac ggcggcggcg cttgctcagc tgcgggcgcg catgacggtc agcgccacca
     5161 ggcgcgagat gaccatggcg acgtacatga cgccggcgag ctgctcgacg gtgccgatgg
     5221 ctcgtgcgtg cggccggatc ggcccgacgt cggacaggcc gacactggtg aggttggccc
     5281 cggagtagta gatgagctcg agcagggtcc gtttcccggg cccgtcgaac gaggtgaacg
     5341 actgcgggta gacctcctgg atggccatga agaggtaggc gaaggcgaac acgagcaccg
     5401 tgaagcaggc accgacggcg aacagctcgt ccttggtgac ccacgagtcc tcgaacatgt
     5461 acgagatgag cgcgtacgcc gtgtagaagt agaagacggc cagcgccgag tgggccaacg
     5521 ccaccaccgt cgagctgtcg tcgatcaggc tccagatctc gaagaccgcc gccggcaggg
     5581 ccagcgttgc cgagagccac gtcagggccg gggtgctgcg caccgtgaac acggcggtga
     5641 tgagcgctga caggctgagc agcgagatga ccgcagcccc ccacgacgac gactcgagca
     5701 cggggagcag gaggatgacg aggatctgca cggcgagcag gaacgccgac ggctgcttgc
     5761 gcagcgtggc cttccatcgg tcgctgcgcg agggcgcctc ctgcaggtcc tcccacatca
     5821 acggcatggc cacagcctag gaccgggtgc gagagaatgc cgccatgccc agcgccctcc
     5881 tgctccccgt cctcacgtgt gccgtcgtgc tcctcgtcag tggtgtcgcc aagctgcgcg
     5941 atccggatgc cgtcgacacg gctttcgtcg acctcgacgt cccagcgggg ctgcgtcacc
     6001 cactgctgcg gcgggccctt ccgtgggtcg agatcgccct cggggtgtgg ctcgtgctcg
     6061 cgaccggctc ggccctggtg gtggtcgcgg cactgaccct ggggttgttc ctggcctacc
     6121 tctggctcgt cgtgcgcgcg gtgcgcatgc ccgaggcggt cgactgcggc tgcttcggcg
     6181 ccctgggcga cacccgggtc acccccgtca ccgtgtggcg caacatcgcg ctcgtggcga
     6241 gcgccgccct gtcgctggcc ctggggctca gcggctccgg cgtgctcgcg gccctgctcg
     6301 aggacccgtc actgtggggg tggcttgcga ccgcggccct ggcagctgcg gtgaccggcc
     6361 tggtgctcta ccgaccgacg gcggatccgg tcaccgccgt cgacggcgtg gtcacgggcg
     6421 agctgcccgt cgacgacgac ggcgactacc tgccgacccc cgcgccggtg gcccagctgc
     6481 tcgacgaggc cggtgctctc gtgctgctct cccgcgagac gccggcggct gcgcacctgc
     6541 tcatcttcct caacccgggg tgcgggtcgt gcgaggccgt caagcccttc gtcgcccagt
     6601 ggagccagga cctcgcaccc gtgacggtgc gggccgtgct catggtcgca ccggctgcgt
     6661 tgacgagcac ctggccggat ctgagcggcc tggcctggtt cgacccgaac ggtgttgccc
     6721 gccgcgcctt cgggtgcgcc acgccgggtg ccgttctcat cggcaccgac ggcaacctcg
     6781 ccggaggccc ggtcaacggg gcccaggcgg tcacggagtt cgtcgacgag atcgctgagc
     6841 agctgcgggc ggcgcacgtc accgggtgag tccacgcgtc gccgcggctg ggtgacgatc
     6901 gggggatgcc gtccgacgcc cagtccacgc gcggggtgac cgtgctgttg cggccctcct
     6961 gtggatgctt cggccgcatc ggcgaccagc gagtgaccgc ccgcacggtg gcggcggtcg
     7021 tcatcgcggc gctcgccgtg ttcatcctcg gcaacgcggc gcagaccccc aaggtggcgc
     7081 gtcccgccgc cggcgccgcg gggtgggcac gtgacctggg ccccgtcgag ctcaccgtcg
     7141 tggtgcccag caccccggcc gagctggctc ggctacccgc ccggcgtgat cgctgcttgc
     7201 gagaagggat gccaagagcg cgaactgccg cgcggttgac acccccggtg tgaacggcac
     7261 gcgcgaaacg ggatgccaca ggcgcaggat ccagtcgaga gatcgcacca cgggcacgac
     7321 atcgctcgat cccgggccag tcttgactcc tcgacctgtc ctgcggcccc ggattctccg
     7381 acctggggcc gagcacgtca ccggcgcccg gtgagctccc acgcgtcctg gtcgccgtcc
     7441 tcgtcgttcc actcgggggc cgcagcgtag gagtccaact ccgccgcgac ggcatccccg
     7501 ccgtacccga ccaccggcac ctcggaggtg tcgtcggtcg acgtgtcggt gacggcggcc
     7561 ggggcggcgt cgaaggtctc ggggacgtcg tcgccccgca tcagcgcgag cgtcgtcggc
     7621 aggtcgtcgg gcttgacgag cacgtcgcgc gccttggagc cctcggacgg gccgacgacc
     7681 ccacgcgact ccagcaggtc catgaggcga ccggccttgg cgaagccgac gcgtagcttg
     7741 cgctggagca tcgaggtcga cccgaactgg gtcgtcacga cgagttcggt cgcctgcagc
     7801 agcaggtcga ggtcgtcgcc gatgtcgtcc tcgacctgct tcttgggtgc ggtgaccgtg
     7861 acgtcgtcgc ggtagcgcgg cttgagctgg tcggtgacgt gcttgacgac gtcgtggatc
     7921 tccgactcgg tgacccaggc accctgcacg cgcatcggct tgctcgcacc catcggcagg
     7981 aagagcgcgt c
//
LOCUS       DAOSOC010000112         7874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9200_length_7874_cov_9.803811, whole genome shotgun sequence.
ACCESSION   DAOSOC010000112 DAOSOC010000000
VERSION     DAOSOC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9200_length_7874_cov_9.803811"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..730)
                     /gene="aroQ"
                     /locus_tag="PL014_09010"
     CDS             complement(290..730)
                     /gene="aroQ"
                     /locus_tag="PL014_09010"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOT57270.1"
                     /translation="MDPRTVLVLNGPNLNLLGEREPEVYGSATLADVEVLCQQAATAS
                     GLVLDFRQSNHEGVLIDWVQEGRAGIAGIVINAAGYTHTSVALRDALSACRVPKVEVH
                     ISDIHAREAFRHHSYLAAVCDHHVIGHGVQGYAEAITWLAGQHR"
     gene            complement(751..>2310)
                     /locus_tag="PL014_09015"
     CDS             complement(751..>2310)
                     /locus_tag="PL014_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOT57271.1"
                     /translation="HVPANPYAPKGPGGPGTLNNLWPERPLPPQTLPVLAALGIGAWA
                     ALTWPERDVGLAVALTMLATGGLMWRVARFRAHPWTLLCAVTAAVLASVTLLRDAGGV
                     IALSVFVAVIVAAAGVSRARTLLAVPLSVIAWPASALRGLPLLARTMSATGRASTLWP
                     VLRTLGFSLIALALFGGLFASGDAVFGAWAQVLVPDLRWDTIIARTFVLALVSGVALT
                     GTYLALNPPNIADAALPATPRARHAWEWAVPVGVVVALFAGFLIAQATAMFGGVEHLR
                     ATTGLTYAEYVHQGFGQLTVATFLTVVVVGLAMRVAPLASSRDRLLLRVLLGALCLLT
                     LAVVASALYRMSLYQEAYGYTVLRVFVDGFELWLGLVIVMLLVAGVRLSGAWVPRAVL
                     GSAAVFVLVFVGLNPDAFVASHNIDRFEAGRELDTAYLARLSADATPVIVERLPADVA
                     SCILDSPGAYTVGGREDDDDLLAWNLGRARAADAVATLGAPTQVPDPTASGERGELCA
                     ALLPPAAVPAG"
     assembly_gap    2311..2410
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2411..3471)
                     /locus_tag="PL014_09020"
     CDS             complement(<2411..3471)
                     /locus_tag="PL014_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT57272.1"
                     /translation="MNTARPLDGITSLKVKLGLLVAVSIVVAVVVERVADAGQVPWWI
                     SVPVTVVAALAVTQWLARGMTSPLREMTSAAGRMAGGDYGQRVTASSSDEVGELARAF
                     NAMAADLASADTQRRQLVATVSHELRTPLTAQRALLENLVDGVVTPDDRALRGALQQS
                     ERLSTLVADLLDLSRIDAGVTSLNLTQVRVADLLSDAVDEARVGARPGRVRHDVLPAD
                     LTVTADRARLAQLVANLLDNAVRHSPADGEVVVTARLVDDSTWCLDVRDDGPGIPADR
                     LERVFDRFGSGDDSGGGTGLGLAIARWVCDLHGGTISAIAPQPGQTGALLRAQLPLTP
                     PTDRPTHLTQERTMSTLTP"
     gene            complement(3468..4253)
                     /locus_tag="PL014_09025"
     CDS             complement(3468..4253)
                     /locus_tag="PL014_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT57273.1"
                     /translation="MTQGPTTPPSGSSAAANASATAATTPSASANTSAAASHVLVVED
                     EPVINQAVTDRLRAEGFHVTQAFDGPGAVDAFTTTSQDLVVLDVMLPGFDGLEVCRRI
                     QAIRPVPVLMLTARTDETDLLVGLGVGADDYVTKPFSMREVVARVRTLLRRVERAAEL
                     AASRPAHLDVQGLRIDVATRRVTAGDAEVHLTPLEFDLLCALAAEPGVVRSRDALMRE
                     VWGWADASGTRTLDSHVKALRAKIGADRVRTVHGVGYALEATP"
     assembly_gap    4345..4444
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4782..5621)
                     /locus_tag="PL014_09030"
     CDS             complement(4782..5621)
                     /locus_tag="PL014_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT57274.1"
                     /translation="MELTHDFTVPAPIDTTWATFMDLHRVGSCFPGAVVTDASDDGFA
                     GTVKVKLGPIALQYAGAGTFLERDDQAYRAVIEAKGKDKRGNGTAGATVTIQLAPSGE
                     GTRVDVTTDLSVTGKPAQFGRGVMQDVSDKLLGQFVACIEQQFAVEDEPAPETVPQPD
                     AGPSAPSAAAGEAGSAGGGTASGSDASGGGAASAGGAVAQSGGTATGAAASVGSTSGL
                     APAASRPAGRSTPRLTSVPEVDDSIDLGATVLPVLLKTYAPHAVVGLIGVLIGWLIAR
                     RRT"
     gene            complement(5630..6478)
                     /locus_tag="PL014_09035"
     CDS             complement(5630..6478)
                     /locus_tag="PL014_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOT57275.1"
                     /translation="MIPAAFDYAAPTTIDEALAALAEAGDEAKVLGGGQSLLPILRMR
                     LNAPEQIVDLGRIDELRGIREDGDHVVIGAMTTYADVIASPLLRTHAALLVQAVEEVA
                     DPQIRRRGTVGGALVHADPAGDVGAPVLALDAELVIRGVDGERTVPVSEFFLDLFETA
                     VGEGELLTAIRIPKHTGWGSHYEKFVRVSHQWSIVGIAVALRVEGGSIAEARVGLTNM
                     GSTPLRAPSVEAALVGRSAADLGDACDTVADGTNPPSDLNGDADYRRHLARVLTRRAV
                     LTALGG"
     gene            complement(6475..>7874)
                     /locus_tag="PL014_09040"
     CDS             complement(6475..>7874)
                     /locus_tag="PL014_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT57276.1"
                     /translation="CQTVLTNKTWVDAYRGAGRPEATYAIERLMDELAVEVGVDPIAI
                     REKNWITHDEFPFTTVAGMTYDSGNYEAATAKALELFGYDELRAEQKARRESGDPVQL
                     GIGVSTFTEMCGLAPSRVLGSLSYGAGGWESASIRMLATGTVEVVSGATPHGQGHETA
                     WSQIVADRLGIPFENVEVLHGDTQIAPKGLDTYGSRSLVVGGEAIVRAADKVIERARP
                     IAAHLLEASVDDLEFAGGRFTVRGTDKGIGITDVALATFAAHNLPDGVEPSLDADATY
                     DPIDFSFPHGTHLCAMEVDTETGATTMRSYVCVDDIGTIVNPLIVEGQIHGGLVQGIA
                     QALFEGAEYDDQGTLVSGSFVDYTLPTAADTINYVSEHTTSPSTTNTLGTKGVGEAGT
                     IASTPAVVNAVVDAVRHLGVADIEMPCTPQRVWKAIRSAGSSGGGAADEAQPHFADGA
                     PNHDPAAGATDGAGQ"
BASE COUNT         1093 a         2601 c         2947 g         1033 t
ORIGIN      
        1 aggtggatga cctcggcgcc ctggctctgc atgatccgcc gcatgatgtt gatcgaggcg
       61 tcgtggccgt cgaacagggc ggatgccgtg acgatccgga ccgggttcac cggcacgtgg
      121 agctcgggct tcgggctggt gggctgcgtt gccatggggg tcccttcggg ttgcctcccg
      181 agatactagg acgtcctagt acctactcgg aagtcaccgg cgctgggccc agcctgccac
      241 tgcgtccggc accttggccg gtggagcacc cacctgccgg acgcagaggt cagcggtgct
      301 ggcccgcgag ccaggtgatc gcctcggcgt agccctgcac cccgtggccg atgacgtggt
      361 ggtcgcacac ggcggcgagg tagctgtggt ggcggaaggc ctcgcgggcg tggatgtcac
      421 tgatgtgcac ctcgaccttg ggcacccggc acgccgacag tgcgtcgcgc agggcgaccg
      481 aggtgtgcgt gtaccccgcg gcgttgatga cgatccccgc gatgccggcc cgcccctcct
      541 gcacccagtc gatgagcacg ccctcgtggt tgctctggcg gaagtcgagc accagaccgg
      601 atgccgtcgc cgcctgctgg cacagcacct cgacgtccgc gagcgtggcg gagccgtaga
      661 cctcgggctc gcgctcaccg agcaggttga ggttcgggcc gttgagcacg agcacggtgc
      721 ggggatccat gacggcgagt atgccgcggg tcagccggca gggacggccg ccggcggcag
      781 cagtgccgcg cagagctcgc cacgctcccc ggatgccgtg gggtcgggaa cctgggtcgg
      841 cgcacccagg gtggccacgg catccgcggc tcgtgcccga ccgaggttcc atgcgagcag
      901 gtcgtcgtcg tcttccctgc cacccacggt gtaggcaccg gggctgtcca ggatgcagga
      961 cgcgacgtcg gcgggcaggc gctccacgat gaccggggtg gcgtcggcgc tcaacctcgc
     1021 caggtaggcg gtgtcgagct cacgcccggc ctcgaagcgg tcgatgttgt gcgaggccac
     1081 gaacgcgtcg gggttcaggc cgacgaagac gagcacgaac acggccgccg atccgagcac
     1141 ggcccgcggc acccacgcgc cgctcagccg cacgccggcg acgagcaaca tcacgatgac
     1201 cagcccgagc cacagctcga agccgtcgac gaagacccgc agcaccgtgt agccgtaggc
     1261 ctcctggtag agcgacatcc gatagagggc gctggcgacg acggccaggg tgagcaggca
     1321 gagcgcgccg aggaggaccc ggagcagcaa ccggtcgcgt gagctcgcga gcggtgcgac
     1381 acgcatcgcc aggccgacga cgacgaccgt gaggaacgtc gccaccgtga gctgaccgaa
     1441 gccctggtgg acgtactccg cgtaggtcag gcccgtggtg gcccgcaggt gctcgacccc
     1501 gccgaacatc gccgtggcct gggcgatcag gaagccggcg aacagggcga cgacgacgcc
     1561 gaccggcacg gcccactccc acgcgtggcg agcgcgcggt gtcgccggca gggcggcatc
     1621 cgcgatgttc ggcgggttca gcgcgaggta ggtgccggtc agtgccaccc cggagacgag
     1681 ggcgaggacg aacgtgcggg cgatgatcgt gtcccagcgc aggtcgggca cgaggacctg
     1741 ggcccaggca ccgaacacgg cgtcaccgga ggcgaacagc ccaccgaaca gggccagtgc
     1801 gatgagggag aacccgaggg tgcgcagcac cggccacagg gtggaggccc tgccggtcgc
     1861 cgacatggtg cgggcgagca ggggcagacc acgcagcgcc gatgccggcc aggcgatcac
     1921 gctgagcgga accgcgagca gggtgcgggc ccggctcacg ccagccgcgg cgacgatgac
     1981 ggcgacgaag acactgagcg cgatgacccc accggcatcg cgcagcaggg tgaccgaggc
     2041 cagcacagcc gcggtgacgg cgcacagcag cgtccacggg tgggcgcgga accgggccac
     2101 ccgccacatg aggccaccgg tggcgagcat ggtcagggcc acggccaggc cgacgtcgcg
     2161 ctcgggccag gtcagcgccg cccaggcgcc gatgccgagg gcggcgagga ccgggagggt
     2221 ctgcgggggc agcggccgct cgggccagag gttgttgagg gtgccggggc cacccgggcc
     2281 cttcggggcg taggggttgg ccgggacgtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn tctggggtca gcgtgctcat ggtgcgctcc tgggtcaggt gggtgggtcg
     2461 gtcagtgggg ggcgtgaggg gcagctgggc tcgcagcagc gcgccggtct gcccgggctg
     2521 gggcgcgatc gcgctgatgg tgccgccgtg caggtcgcac acccagcggg cgatggccag
     2581 gccgaggccg gtgccgccgc cggagtcgtc gccggagccg aagcggtcga agacccgctc
     2641 gagccggtcg gccgggatgc cggggccatc gtcacgcacg tcgaggcacc acgtggagtc
     2701 atcgaccagt cgcgcggtga cgacgacctc gccgtcggcc gggctgtggc gcacggcgtt
     2761 gtcgagcagg ttcgcgacga gctgggccag gcgcgcccgg tcggccgtca ccgtgaggtc
     2821 cgccggcagc acgtcgtgac gcacgcgtcc ggggcgagcg cccacgcgag cctcgtccac
     2881 ggcatccgag agaaggtccg cgaccctgac ctgcgtgagg ttcagagagg tgaccccggc
     2941 gtcgatgcgc gagaggtcga gcaggtccgc gacgagggtg ctgaggcgct cggactgctg
     3001 cagcgcgccg cgcagggccc ggtcgtccgg ggtgacgacc ccgtcgacga ggttctcgag
     3061 cagggcccgc tgggcagtga ggggggtgcg cagctcgtgc gacaccgtgg ccacgagctg
     3121 gcggcgctgg gtgtcggcgg aggccagatc cgcggccatg gcgttgaagg cccgcgccag
     3181 ctccccgacc tcgtcggagg aggatgccgt gacgcgctgt ccgtagtcgc ctcccgccat
     3241 gcgccccgcg gccgacgtca tctcgcgcag cggtgaggtc atgccgcgcg cgagccactg
     3301 ggtgaccgcg agggcggcga ccacggtgac ggggacgctg atccaccacg gcacctgacc
     3361 ggcatccgcc acccgctcga cgacgacggc cacgacgatg ctgacggcga cgagcaggcc
     3421 gagcttgacc ttgagcgagg tgatgccgtc gagcgggcgg gcggtgttca cggggtggcc
     3481 tcgagggcgt acccgacgcc gtgcacggtg cgcacgcggt cggcgccgat cttggcgcgc
     3541 agggccttga cgtggctgtc gagggtgcgg gtgcccgagg cgtcggccca gccccagacc
     3601 tcgcgcatca gggcgtcgcg ggagcggacc accccgggtt cggcggccag ggcgcacagc
     3661 aggtcgaact ccagcggggt caggtgcacc tcggcgtctc cggcggtgac gcgccgggtg
     3721 gcgacgtcga tgcgcaggcc ctgcacgtcg aggtgggccg ggcggctggc tgcgagctcg
     3781 gcggcccgct cgacacggcg cagcagggtg cgcacccggg cgacgacctc gcgcatcgag
     3841 aacggcttgg tcacgtagtc gtcggcgccg accccgagac cgacgagcag gtcggtctcg
     3901 tcggtgcggg cggtgagcat gagcaccggc accgggcgga tggcctggat gcggcgacac
     3961 acctcgaggc cgtcgaaccc gggcagcatg acgtcgagga cgacgaggtc ttggctggtg
     4021 gtggtgaacg cgtcgacggc gccggggccg tcgaaggcct gcgtgacgtg gaagccctcg
     4081 gcgcgcagcc ggtcggtgac ggcctggttg atgaccggct cgtcctcgac gacgagcacg
     4141 tgggatgccg ccgcgctggt gttggcgctc gcgctcggcg tcgtcgctgc ggtggccgag
     4201 gcgttcgctg cggctgatga tccgctcggc ggagtggtgg ggccctgggt catgctcccg
     4261 agcgtaggca ggtgcggctg gagcccgcgg gggcgacgtg tggagatcgt gtgaaggtgc
     4321 caccccgccc cgacttcgtg cgacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnggggcg acgtgtggag atcgtgtgaa ggtgccaccc cgccccgact tcgtgcgact
     4501 tccgtgcggc acctgagcgg atcagggccc acaagcgcac cgaagtcgca cgaagtcggg
     4561 aaggcagtgg ccctcagcag cgcccggggg ccaagggcgc gaccagccgc gcgtctggca
     4621 ccccacccgg cattgggtga cgatcaggcg cccctcaccc gggctccccc gcgaatgtgg
     4681 gtgacgttcg tcgcccaggc gacgatcttc acccacattc gccggaggga ggggcggccc
     4741 ggagggagga gtgcgggcgg agcaggcacc gacaggaaag gtcaggtgcg gcggcgggcg
     4801 atgagccagc cgatgagcac gccgatcagg cccaccacgg cgtgcggggc gtaggtcttc
     4861 agcagcaccg gcagcacggt cgcgccgagg tcgatcgagt cgtccacctc aggcaccgac
     4921 gtcaaccgcg gggtggagcg gcccgccggc cgggacgcag ccggggccag ccccgaggtg
     4981 gagccgacgg atgccgcggc accggttgcc gtgcctccgc tctgggcgac cgcaccgcct
     5041 gcagatgccg cgccaccgcc ggatgcgtca ctaccggatg ccgtgccacc acccgcgctg
     5101 cccgcctcac cggcggccgc gctgggagcc gacggcccgg catccggctg aggcacggtc
     5161 tcgggcgcgg gctcgtcctc cacggcgaac tgctgctcga tgcacgcgac gaactggccg
     5221 agcagcttgt cggagacgtc ctgcatcacc ccgcgcccga actgcgccgg cttgccggtg
     5281 accgacaggt cggtcgtgac gtcgacccgg gtgccctcac ccgagggcgc gagctggatc
     5341 gtcacggtgg cgccggccgt gccgttgccc cgcttgtcct tgcccttggc ctcgatgacg
     5401 gcccggtacg cctggtcgtc acgctcgagg aacgtgccgg caccggcgta ctggagcgcg
     5461 atggggccga gcttgacctt caccgtgccg gcgaaaccgt cgtcgctggc gtcggtgacg
     5521 accgcgccgg ggaagcagct gccgacccgg tgcaggtcca tgaaggtcgc ccaggtggtg
     5581 tcgatcgggg cggggacggt gaagtcgtgc gtcagttcca tgcggtgact cagcctccga
     5641 gcgcggtgag cacggcgcga cgggtgagca cccgcgcgag gtgccgccgg tagtcggcat
     5701 caccgttcaa gtcgctcggc gggttcgttc cgtcggcgac ggtgtcgcag gcgtcaccga
     5761 ggtccgcggc cgaccggccc acgagggcgg cctcgaccga gggcgcccgc aacggggtcg
     5821 agcccatgtt ggtcaggccg acccgggcct cggcgatcga gccgccctcg acgcgcaggg
     5881 ccaccgcgat gccgacgatc gaccactggt gggacacgcg cacgaacttc tcgtagtgcg
     5941 acccccaacc ggtgtgcttg gggatgcgga tggccgtgag caactcaccc tcaccgacgg
     6001 cggtctcgaa caggtcgagg aagaactcgc tgaccggcac cgtgcgctca ccgtccacgc
     6061 cgcggatgac gagctcggcg tcgagcgcga gcacgggggc accgacgtct cccgcggggt
     6121 cggcgtgcac cagggcaccg ccgacggtgc cgcggcgccg gatctgcggg tcggcgacct
     6181 cctcgacggc ctgcacgagc agcgccgcgt gggtgcgcag gagcgggctc gcgatgacgt
     6241 cggcatacgt cgtcatcgcg ccgatgacga cgtggtcgcc gtcctcgcgg atgccgcgca
     6301 gctcgtcgat gcgtccgagg tcgacgatct gctcgggcgc gttgaggcgc atgcgcagga
     6361 tcggcaggag cgactgccca ccgccgagga ccttggcctc gtcgcctgcc tcggccagcg
     6421 cggccagggc ctcgtcgatc gtcgtggggg cggcgtagtc gaatgctgcg gggatcactg
     6481 gcctgctccg tccgtcgcgc ctgctgctgg atcgtggttg ggtgcgccgt cggcgaagtg
     6541 cggctgagcc tcgtcggccg ctccgccacc cgacgacccg gccgaccgga tggccttcca
     6601 cacccgctgg ggcgtacacg gcatctcgat gtcggcgacg ccgaggtgac gcacggcatc
     6661 cacgacggcg ttgacgaccg cgggcgtcga ggcgatggtg ccggcctcgc cgaccccctt
     6721 ggtgccgagg gtgttcgtcg tcgacggcga ggtggtgtgc tcgctgacgt agttgatcgt
     6781 gtcggccgcg gtcgggagcg tgtagtcgac gaacgagccg gacaccaagg tgccctggtc
     6841 gtcgtactcg gcgccctcga acagcgcctg cgcgatgccc tggaccaacc cgccgtggat
     6901 ctgtccctcg acgatcagcg ggttgacgat ggtgccgatg tcgtcgacgc acacgtagga
     6961 gcgcatcgtc gtggcaccgg tctcggtgtc gacctccatc gcgcacaggt gagtgccgtg
     7021 cgggaaggag aagtcgatcg ggtcgtaggt ggcgtcggcg tcgaggctgg gctcgacccc
     7081 gtcgggcagg ttgtgcgccg cgaaggtcgc gagcgccacg tcggtgatgc cgatgccctt
     7141 gtcggtgccg cgcaccgtga accgaccacc cgcgaactcg aggtcgtcga cgctggcctc
     7201 gagcagatgg gccgcgatgg ggcgggccct ctcgatgacc ttgtcggcgg cccgcacgat
     7261 ggcctcaccg ccgacgacga gcgagcgcga cccgtaggtg tccaggccct tgggcgcgat
     7321 ctgggtgtcg ccgtgcagca cctcgacgtt ctcgaagggg atgccgagcc ggtcggcgac
     7381 gatctggctc cacgccgtct cgtggccctg cccgtgcggg gtggcccccg agacgacctc
     7441 gacggtgccg gtcgcgagca tccggatgct ggcgctctcc cagccgccgg cgccgtagct
     7501 gagcgagccg agcacccgcg agggcgccag gccgcacatc tcggtgaacg tcgagacgcc
     7561 gatgccgagc tgcacggggt cgccggactc gcgacgggcc ttctgctcgg cgcgcagctc
     7621 gtcgtagccg aagagctcga gcgccttggc ggtggcggcc tcgtagttgc ccgagtcgta
     7681 ggtcatgccc gcgaccgtgg tgaacgggaa ctcgtcgtgg gtgatccagt tcttctcgcg
     7741 gatggcgatc gggtcgaccc cgacctcgac ggcgagctcg tccatgagcc gctcgatggc
     7801 gtaggtggcc tcgggccgcc cggcgccgcg gtaggcgtcg acccaggtct tgttcgtcag
     7861 cacggtctgg cagt
//
LOCUS       DAOSOC010000113         7841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9245_length_7841_cov_6.578474, whole genome shotgun sequence.
ACCESSION   DAOSOC010000113 DAOSOC010000000
VERSION     DAOSOC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9245_length_7841_cov_6.578474"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..758)
                     /locus_tag="PL014_09045"
     CDS             complement(114..758)
                     /locus_tag="PL014_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT57277.1"
                     /translation="MSKRDQEPAATTPATTADGPDEHTLAVRRRLEDAMAAAVTQKGL
                     AATTIADVARHAGTSKRTFYEHYADKTQCYLSLFATRSAAMVDEVDAALRPGELPATV
                     QLANAARTFLGVVTTDPVLGRGHLSELATLGEEAAQVRREVVDRQATSILEMLERARA
                     GGTAVRRISHLEAVCVVGGLNEIGLRAVDPGSRIATDDLVETATRFMTAVLLAD"
     gene            844..1476
                     /locus_tag="PL014_09050"
     CDS             844..1476
                     /locus_tag="PL014_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57278.1"
                     /translation="MQLVVDEAAVAGARSPLVQATSFVAPSSRVTPVAGDPGYGQVAL
                     ALALAGRMPLAGGRVTLDGSDDPAVLRRHVAVVDVPGVSAPEDGLAVHHVLAEELAFA
                     GRPSGRSAVEAFLTAHGVGAAADAPFETLAPEDRVRVLAHAAAERTATRVLVITNPDR
                     RGGDARTWWPVLEALAADGITVVVQLTHATMRQLDLPVRAELGCVRGVLA"
     gene            1473..3452
                     /locus_tag="PL014_09055"
     CDS             1473..3452
                     /locus_tag="PL014_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HOT57279.1"
                     /translation="MTALRLALTELRRIGASRVGRLALVAMVLVPSIYGGLYLYANND
                     PYGGLNRVPAALVVEDTGTTLSTGERLEVGDQVADKLVDAGTFDWARVDRARADAGLE
                     AGDYDLVLVVPASFSQDLASSATNDPRQATLQIRTNDANNYLSRTIANTLVSQVTASV
                     AEQVSSTAASRFLEGFADIHAEVTKASDGAAKLADGATTAAKGAGALADGADQLVAGQ
                     QELVTGTDALASGAGDLSDGLGTLRDATADLPAQTTKLADGARQVSDGDAKVAAAGRD
                     VADAADGLLGDLTASRGRLADQLRAAGFTEAQITTVLAKVDGLSGPLEDANTRVQSTA
                     DDLDRLASGADQVADGAERLAASAPKLSGGIADAAAGSTKLATGADQLAAGQRTALDG
                     ATRLASGAHDLDDGLGTLADGANELSDGLAKGAASIPDPSAEQRKAMAQTIGSPVTVD
                     RDAEARAASYGAGLAPFFMSLALWIGGFVLFTRMRALSTRALAAGQPGWRVALGGWLG
                     AALLGAAQAVVAFGVVARGVGIDVANPWLLVLFMVGVSMAFIAIIHALMARFGVVGQF
                     MALVLMVLQLVSAGGTFPWQSLPEPLHPLHQALPMSYAVDGVRRLMYGGPLGQLWLDV
                     AVIGAWAVGALVFSSLAARRAGTWTAARVKPELVA"
     gene            complement(3514..4116)
                     /locus_tag="PL014_09060"
     CDS             complement(3514..4116)
                     /locus_tag="PL014_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57280.1"
                     /translation="MPRDAAAIDSRSRRAHDPEVTLFDTIGGIPLHPLIVHAVVVLVP
                     LASLLLLLAAVSPRIRHWAGILPPITATLALVMVPLATQSGESLERRVEASPALEEHT
                     ELGDSLLYFVIVLAILAWALWFLDRRAQAAVTTGADASDAAHGTGTAARSPLLTVVIV
                     LSVISVLATTVQVVRIGHSGASASWSDVGAKTVPAGGGDD"
     gene            complement(4204..5175)
                     /locus_tag="PL014_09065"
     CDS             complement(4204..5175)
                     /locus_tag="PL014_09065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HOT57281.1"
                     /translation="MRAAQVTRLDGPDAVEVVDLPEPAGDGVVIEVAAAGICFPDVLL
                     TRGQYQITPDLPFVPGSEVAGVVRSAPTSSGYAAGDRVAAFPFLGGFAEVVEADPAMV
                     FPLPDSVTYEQGAALPMNYLTCHFALRERGRLREGETVLVQGAAGGIGTAAVQLAAAW
                     GARIIAVVSSQDKGDVARAAGAHEVVLADGFRDAVSALTNGRGVDVVVDPVGGDRFTD
                     SLRTLAPGGRLLVIGFTAGSIPEVRVNRLLLGNISVVGVGWGAWWTGRGGPGPAFLRR
                     QWDDLVPLLESGAIDPVIGEVRDLEDVVAALTAVDERRAMGKILLAP"
     gene            5278..5667
                     /locus_tag="PL014_09070"
     CDS             5278..5667
                     /locus_tag="PL014_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT57282.1"
                     /translation="MIEFSLDGRSGVSPYLQVVHQVRQALRLGLLREGDQLPTVKDVV
                     ATLAINPNTVLKAYRELERDGLVTARPGVGTFVTRTLADDSLAAHEPLRRELQRWLVK
                     ARAAGVDDESIEALFRSAFRGGQRGVA"
     gene            5664..6578
                     /locus_tag="PL014_09075"
     CDS             5664..6578
                     /locus_tag="PL014_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57283.1"
                     /translation="MTAAIATDALGKRYRRRWALSDCTLDIPAGRVTGLVGQNGAGKS
                     TLLNLAAGLLRPTTGTIEVCGGRPATDAAQLAKVGFVAQDTPTYAGLSIRDHLRLGAR
                     LNPGWDESLAASRVERLGLDPGQKTGRLSGGLRAQLAFALGLAKRPELLILDEPVASL
                     DPFARREFLQVLMEAVAEQELSVVLSSHVVSDLERVCDHVVVLVDSQVRLAGEVEALL
                     DTHHRLTGPRRTTREMPRDQQVVSERHTDRQTTLVVRTEGPVLDPSWTVTQLGLEDLV
                     LSYMELPTSDLTGRGDGTTGAPSLEVLK"
     gene            6575..7597
                     /locus_tag="PL014_09080"
     CDS             6575..7597
                     /locus_tag="PL014_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57284.1"
                     /translation="MIWLTWRQLRAQAATVVLAVTAMAVVLALTGPRLVELARAGADE
                     VFDRLTSTDGTLFWAGLVVVAAVPALVGAFWGAPLVARELEAGTHRLVWNQSVTRTRW
                     LLTKLAVTTAVAALAVGALSFAVTWWSDPLDGAVGHARGSFPGVLTPVSFAMRGVAPV
                     GYTVFAVVLGVTLGAVLRRSLTAMALTLAVYVLVQLAVPLWVRPYLVAPTTATIAISA
                     RTLDSVVADDQGGPQSITVHTTDRGDWIVANETLAADGRPAALPAWMSDCFAAAPDRF
                     AGPIEASPINPCLERLASEGYTQRVVYQPAGNFWPVQLAETALYLAASGLLAGFCLWW
                     TRRRLS"
BASE COUNT         1008 a         2895 c         2889 g         1049 t
ORIGIN      
        1 cggccagcgc cacgggcacg gtgcacgagg cgacgcgggc gaacccctgg gcgtacgcgt
       61 tacggaagtc catgggccga gcctattgct ccgggtgaac cctcgatgag cggtcagtcg
      121 gcgagcagca cggcggtcat gaaccgggtg gccgtctcga cgaggtcgtc ggtggcgatc
      181 cgcgacccgg ggtcgacggc gcgcagcccg atctcgttga gcccaccgac cacgcagacc
      241 gcctcgaggt ggctgatccg gcgcaccgcg gtgccacccg cgcgggcccg ctcgagcatc
      301 tccaggatcg acgtcgcctg ccggtcgacg acctcgcggc gcacctgcgc ggcctcctcg
      361 ccgagggtgg ccagctcgct gaggtgcccg cgcccgagta ccgggtcggt ggtgacgacg
      421 ccgaggaagg tgcgcgcggc gttggccagc tgcaccgtcg cgggcagctc accgggccgc
      481 agcgcggcat ccacctcgtc gaccatcgcc gcactgcggg tggcgaacag cgacaggtag
      541 cactgcgtct tgtcggcgta gtgctcgtag aaggtccgct tggaggttcc cgcgtgccgc
      601 gccacgtctg cgatcgtcgt ggcggccagt cccttctgcg tgacggcggc agccatcgcg
      661 tcctccagcc ggcggcgcac ggcgagcgtg tgctcgtcgg ggccgtcggc ggtggtcgcc
      721 ggcgtcgtcg cggcgggttc ctggtcgcgt ttgctcatgc tggtaccgca ccgtaccatc
      781 ggaggtggta ccaatcggta ccagcggtgt cagccaccgc accgtcaccg gaaggagcag
      841 gccgtgcagc tcgtcgtcga cgaggccgcc gtcgccggcg cccgcagccc cctcgtacag
      901 gcgacctcgt ttgtcgcccc gtcctcgcgg gtcacgcccg tggcgggtga cccgggctac
      961 ggccaggtgg cgctcgccct cgcgctcgcc gggcggatgc cgctcgccgg gggtcgggtg
     1021 acgctcgacg gctccgacga cccggccgtg ctgcggcggc acgtcgccgt cgtcgacgtg
     1081 ccgggggtca gtgctcccga ggacggcctc gcggtgcacc acgtgctcgc cgaggagctc
     1141 gccttcgccg ggcgcccgtc cggtcgcagc gcggtcgagg cgttcctcac ggcccacggg
     1201 gtcggcgccg ccgccgacgc gccgttcgag acgctcgcgc ccgaggaccg tgtgcgggtg
     1261 ctcgcccacg ccgccgccga gcgcaccgcc acccgcgtgc tcgtcatcac caaccccgac
     1321 cgccgtggcg gtgacgctcg cacctggtgg ccggtgctcg aggccctcgc cgcggacggc
     1381 atcacggtgg tcgtgcagct cacccacgcc accatgcgcc agctcgacct gccggtgcgg
     1441 gccgagctcg gctgtgtccg gggggtgctc gcgtgaccgc cctgcgcctc gccctcaccg
     1501 agctgcgccg catcggtgcc agccgggtcg gccgcctggc cctggtcgcc atggtgctcg
     1561 tgccgtcgat ctacggcggc ctctacctct acgccaacaa cgacccctac ggcgggctga
     1621 accgggtgcc ggcggccctc gtcgtcgagg acaccggaac cacgctgtcg accggcgagc
     1681 ggctcgaggt cggtgaccag gtggccgaca agctcgtcga cgccggcacc ttcgactggg
     1741 cacgcgtcga ccgcgcccgg gccgatgccg gcctcgaggc gggcgactac gacctcgtgc
     1801 tggtcgtccc ggcgagcttc tcgcaggacc tcgcctccag tgccaccaac gacccccgcc
     1861 aggcgacgct gcagatccgc accaacgacg ccaacaacta cctgtcccgc accatcgcga
     1921 acaccctggt cagccaggtc accgcctcgg tggccgagca ggtcagctcg accgcggcga
     1981 gccgcttcct cgagggcttc gccgacatcc acgccgaggt caccaaggcc tcggacggcg
     2041 ctgcgaagct cgccgacggt gccacgacgg cggcgaaggg agcgggagcc ctggccgacg
     2101 gcgccgacca gctcgtcgcg ggccagcagg agctcgtcac cggtaccgac gcgctggcct
     2161 ccggcgccgg agacctctcc gacgggctcg gcacgctgcg cgacgccacc gccgacctcc
     2221 ccgcccagac cacgaagctg gccgacggcg cccggcaggt gtccgacggc gacgcgaagg
     2281 tcgctgccgc gggccgggac gtggcggatg ccgcggacgg cctgctcggt gacctcacgg
     2341 catcccgagg tcgcctcgcc gaccagctgc gcgcggccgg cttcaccgag gcgcagatca
     2401 ccaccgtgct cgcgaaggtg gacgggctgt ccgggccgct cgaggacgcc aacacgaggg
     2461 tgcagtcgac cgccgacgac ctcgaccggc tggcctccgg cgccgaccag gtcgccgacg
     2521 gcgccgagcg gctcgcggcc tcggccccga agctgtcggg gggaatcgcg gacgccgcgg
     2581 ccggcagcac gaagctggcc accggtgccg accagctcgc cgcggggcag cgcaccgccc
     2641 tcgacggggc gacccggctc gcgtccggcg cgcacgacct cgacgacggg ctcggcacgc
     2701 tggccgacgg cgcgaacgag ctctccgacg ggctcgcgaa gggcgcagcc tccatccccg
     2761 acccctccgc cgagcagcgc aaggccatgg cgcagaccat cggcagcccg gtgacggtcg
     2821 accgtgacgc cgaggcgcgg gcggccagct acggcgccgg cctggcgccg ttcttcatga
     2881 gcctggccct gtggatcggc ggcttcgtgc tcttcacccg gatgcgggcg ctgtcgaccc
     2941 gggcgctggc cgcgggccag cccgggtggc gggtcgcgct cggcggctgg ctcggtgccg
     3001 cgctgctcgg tgccgcccag gccgtggtgg ccttcggggt cgtcgcgcgg ggcgtgggca
     3061 tcgacgtggc caacccctgg ctgctcgtgc tgttcatggt cggggtctcg atggcgttca
     3121 tcgcgatcat ccacgcgctg atggcccgct tcggcgtggt ggggcagttc atggccctgg
     3181 tgctgatggt gctccagctg gtcagcgccg gtggaacctt cccctggcag tcgctaccgg
     3241 agccgctgca cccgctgcac caggcgctgc cgatgtccta cgccgtcgac ggggtgcggc
     3301 gcctgatgta cggcggcccg ctcggccagc tgtggctcga cgtcgccgtc atcggtgcgt
     3361 gggcggtcgg cgccctggtc ttctcgtcac tggccgcgcg gcgggccggc acgtggaccg
     3421 ccgcacgggt aaagcccgag ctggtcgcct gacgacgacg gcccggcgag cggtcgcaac
     3481 cgctggccgg gccgtcagcc gacgggctgg ggatcagtcg tcgccgccac cggcgggcac
     3541 ggtcttcgcg ccgacgtcgg accacgaggc ggaggcgccg ctgtggccga tccggacgac
     3601 ctgcacggtg gtcgcgagca ccgagatcac cgagagcacg atgacgacgg tcagcagggg
     3661 cgaccgagcg gccgtccccg tgccgtgagc ggcgtcggag gcgtccgccc cggtggtgac
     3721 cgccgcctgg gcgcggcggt cgaggaacca cagcgcccac gcgaggatcg cgaggacgat
     3781 cacgaagtag agcaggctgt cgccgagctc ggtgtgctcc tcgagggcgg ggctcgcctc
     3841 gacgcggcgt tcgagggact cgccgctctg ggtggccagc ggcaccatga ccagcgcgag
     3901 ggtcgccgtg atcggcggga ggatgccggc ccagtgccgg atccgcggcg agaccgcggc
     3961 gagcagcagc aggagtgacg cgagcggcac gaggaccacg acggcgtgga cgatgagcgg
     4021 gtgcaggggg attccgccga tggtgtcgaa caaggtgacc tccgggtcgt gggcgcggcg
     4081 agagcgcgag tcgatggccg cggcatcccg cggcactgcc cccacccttg ccgtcgacgc
     4141 gcggaaactc cctaagacag tcctaaggga acgtgagggt tgcgagtggc cctgtcggtt
     4201 gggtcacggt gccagcagga tcttgcccat ggcgcggcgc tcgtcgaccg cggtgagcgc
     4261 cgccacgacg tcctcgaggt cgcgcacctc gccgatgacc gggtcgatcg cacccgactc
     4321 gagcagcgga acgaggtcgt cccactgccg acgcaggaag gccggcccgg ggccgccgcg
     4381 cccggtccac cacgcgcccc agccgacgcc gacgaccgag atgttgccga gcagcagccg
     4441 gttgaccctg acctcgggga tcgacccggc ggtgaacccg atgacgagca gccgcccacc
     4501 gggcgcgagc gtgcgcagcg agtcggtgaa ccggtcgccg cccacggggt cgaccacgac
     4561 gtcgacgcca cgcccgttgg tgagcgcgga cacggcatcg cggaaaccgt cggcgagcac
     4621 cacctcgtgg gccccggcgg cccgcgccac gtcgcccttg tcctgcgacg acacgacggc
     4681 gatgatccgg gcgccccacg cggcggcgag ctgcaccgcc gcggtgccga tgcccccggc
     4741 cgcgccctgc acgagcaccg tctcgccctc gcgcaaccgg ccccgctcgc gcagggcgaa
     4801 gtggcaggtg aggtagttca tcggcagcgc ggcgccctgc tcgtaggtga ccgagtcggg
     4861 cagcgggaag accatcgccg ggtcggcctc caccacctcc gcgaacccac cgaggaaggg
     4921 aaaggccgcg acccggtcgc ccgcggcgta cccgctgctc gtcggcgccg agcgcaccac
     4981 gccggccacc tcggacccgg ggacgaacgg caggtcgggg gtgatctggt actgcccacg
     5041 ggtgagcagc acgtcgggga agcagatgcc ggccgccgcg acctcgatga cgacgccgtc
     5101 gcccgcgggt tcggggaggt cgaccacctc caccgcgtcg ggtccgtcga gtcgggtcac
     5161 ctgggctgcg cgcacctcgc catcgtaggg acgtaccggg tgcgtggtcg gatgagggtt
     5221 gtcagggtca cggcgctcac ctattgtgct agtcaactag gtgaatagga tgccgtgatg
     5281 atcgagttct cgctggacgg caggtccggg gtctcgccct acctgcaggt ggtgcaccag
     5341 gtccgccagg cgctgcggct cggcctgctg cgcgagggtg accagctgcc cacggtcaag
     5401 gacgtcgtgg ccacgttggc gatcaacccc aacaccgtgc tcaaggccta ccgcgagctc
     5461 gagcgcgacg gcctggtgac ggcccgtccc ggcgtgggca ccttcgtcac ccgcacgctc
     5521 gcggacgact ccctggccgc ccacgagccg ctgcgccgcg agctgcagcg gtggctggtg
     5581 aaggcgcggg ccgcgggggt cgacgacgag agcatcgagg cgctgttccg cagcgccttt
     5641 cgcggcgggc agcgaggcgt ggcgtgacgg ccgcgattgc cactgacgcc ctgggcaagc
     5701 gctaccggcg gcgctgggcg ctgtcggact gcaccctgga catccccgcc gggcgggtca
     5761 ccgggctggt gggccagaac ggcgcgggaa agagcaccct gctcaacctc gcggccgggc
     5821 tgctgcggcc gacgaccggc acgatcgagg tctgcggcgg gcgacccgcg acggatgccg
     5881 cccagctcgc gaaggtcggc ttcgtcgccc aggacacccc gacgtacgcc gggctgagca
     5941 tccgcgacca cctgcggctg ggcgcccggc tcaaccccgg gtgggacgag tccctcgcgg
     6001 cgtcgcgggt cgaacggctc gggctcgacc ccgggcagaa gacaggtcgg ctgtccggcg
     6061 ggctgcgcgc ccagctcgcg ttcgcgctgg ggttggccaa gcgccccgag ctgctcatcc
     6121 tcgacgagcc ggtcgcgagc ctcgacccgt tcgcccggcg ggagttcctc caggtgctga
     6181 tggaggcggt cgccgagcag gagctcagcg tggtgctgtc ctcgcacgtg gtgtccgacc
     6241 tcgagcgggt ctgcgaccac gtggtggtgc tcgtcgactc gcaggtgcgg ctcgccggcg
     6301 aggtcgaggc gctgctcgac acgcaccacc ggctgaccgg tccgcgacgg acgacccggg
     6361 agatgccgcg tgaccagcag gtggtgtcgg agcggcacac cgaccggcag accaccctcg
     6421 tggtccggac cgagggcccc gtcctcgacc cgtcctggac cgtcacccag ctgggcctcg
     6481 aggacctcgt cctctcgtac atggagctgc ccacgtcgga cctgaccggc cgcggcgacg
     6541 ggacgaccgg cgcgccgtcg ctggaggtgc tgaagtgatc tggctgacct ggcgccagct
     6601 gcgcgcgcag gccgcgaccg tcgtcctggc cgtgaccgcg atggcagtcg tgctcgccct
     6661 caccggcccg cgcctggtcg agctggcgag ggcgggcgcc gacgaggtgt tcgaccggct
     6721 caccagcacc gacggcaccc tcttctgggc ggggctggtc gtcgtcgccg cggtccccgc
     6781 cctggtgggg gccttctggg gcgcgccgct ggtggcccgc gagctggagg ccggcaccca
     6841 ccgtctggtg tggaaccaga gcgtcacccg cacccgctgg ctcctcacga agctggccgt
     6901 caccacggcg gttgccgcgc tggcggtggg agcgttgagc ttcgcggtga cctggtggtc
     6961 ggatcctctc gacggggccg tggggcacgc gcggggcagc ttccccggag tgctcacccc
     7021 ggtgtcgttc gcgatgcgcg gcgtggcacc ggtcggctac accgtgttcg cggtcgtgct
     7081 cggggtgacc ctgggcgccg tcctgcggcg ttccctgacc gccatggcgc tgacgctggc
     7141 ggtctacgtg ctggtgcagc tggcggtgcc gctctgggtg cggccgtacc tcgtcgcgcc
     7201 gacgacggcg accatcgcga tcagtgcccg gaccctcgac tcggtcgtgg ccgacgacca
     7261 gggcggcccc cagtcgatca cggtgcacac gaccgatcga ggggactgga tcgtcgccaa
     7321 cgagacgctg gctgcagacg ggcggcctgc cgcgctgccc gcctggatgt ccgactgctt
     7381 cgccgcggca ccggaccgct tcgccggccc gatcgaggct tcgccgatca acccctgcct
     7441 cgagcggctg gcgagcgagg gctacacgca gcgcgtggtc taccagccgg ccggcaactt
     7501 ctggccggtc cagctcgccg agaccgcgct ctacctcgca gcatcagggc tgctcgccgg
     7561 cttctgcctc tggtggacgc gccgccggct gtcctgaacc ggccgcgacg ggcgaggcgg
     7621 gctgcgcgcc cgaccccacg gcatccgtcg tgcccggtga gcttcgcgtg acatcgaccg
     7681 aaaggtccgt tcggcgggaa tgcggtcggg ggtgccgggc gctgcacgtg cagtgcccca
     7741 accccgccac gccaccctcg cgaccctcgc cctgatcctg ggcggggtcg ccatcggcac
     7801 caccgagttc gtgacgatgg gactgctgcc cgagatcgcc g
//
LOCUS       DAOSOC010000114         7806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9305_length_7806_cov_9.564701, whole genome shotgun sequence.
ACCESSION   DAOSOC010000114 DAOSOC010000000
VERSION     DAOSOC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9305_length_7806_cov_9.564701"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1356)
                     /locus_tag="PL014_09085"
     CDS             complement(121..1356)
                     /locus_tag="PL014_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOT57285.1"
                     /translation="MTTADVAAAGPDKKRLNLAPLQKFGRSLMLPIAALPVAALMLRL
                     GQPDLLGRDGLGWEKVAAVVGAGGGALFANLPLLFAVGVAIGVAKKADGSTALAAVVG
                     YLVFKGVGDAVSPFILPAPKPGEDQELVNYGVLGGILCGLIAAVLWQRYYRISLPPYL
                     AFFGGRRFVPIVTGFATLVLAVLLAIIYPAFDAVLTGLGEWVAANSIIGGFIYGTANR
                     LLIPLGLHHILNSVPWFVLGEYTPAGGDTVQGDIARFLKGDPTAGAFMSGFFPIMMFA
                     LPAAALAIYQEAKPKAKKAVGGLMASVALTAFLTGVTEPIEFAFMFVAWPLYVVHAVL
                     TGTSMALANALEIRDGFGFSAGLFDFLLNWNIATRPALLIVMGLGYAVVYYVLFRFVI
                     RRWNLRTPGREDDADDAAS"
     gene            1443..1676
                     /locus_tag="PL014_09090"
     CDS             1443..1676
                     /locus_tag="PL014_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose/sucrose transporter subunit IIB"
                     /protein_id="HOT57286.1"
                     /translation="MSSKAQQILIALGGADNIIEIEPCITRLRTEVKDPTVVDEAALK
                     EAGAHGVIRAGSVVQVVVGPEADNLAEDIEDLM"
     gene            1673..2224
                     /locus_tag="PL014_09095"
     CDS             1673..2224
                     /locus_tag="PL014_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT57287.1"
                     /translation="MSRPQALLLDLDGTLVDSEPIHRAGFERWFAHRGWAFDREVASL
                     FTGRRADDVFRTVDGPWSGGDPTEMFHEIVAMVPRDRLPDAVPGAVASIERALAAGIP
                     LALVTSAGAGGPTRRWPRSGASTGSTSSSPATTSPWASPTRRGTPSPANDSGWMPPAP
                     SRARTHLRGWSPPSLPEWEPSSG"
     gene            complement(2477..4147)
                     /locus_tag="PL014_09100"
     CDS             complement(2477..4147)
                     /locus_tag="PL014_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HOT57288.1"
                     /translation="MSTSSPAADAASATAVDAPAGPLSHRQIVTILIGLMMGMFLAAL
                     DQTIVATAIRTIADDLQGLNHQAWATTAYLITSTIVTPLYGKLGDIYGRKKLFIIAIS
                     LFILGSVLCTFATSMGSLAFYRAVQGLGAGGLFSLALAIIGDIVPPRERAKYQGYFLA
                     VFGTASVLGPVIGGFFAGQSSILGIDGWRWVFLVNVPIGIAALAVVTKTLHLRHTRLD
                     HRIDWAGAALLSLSLVPLLLVAEQGREWGWGSTNAIICYVIGVVAAIGFFWQEHRMGE
                     EAILPLAMFRNKTVGVSSIASVLIGVAMFGGLAALPLYLQIVKGATPTEAGLLLLPMT
                     LGIMIGSILSGQIISRTGRYRVFPIVGSALLTISLFIFHYVAYDTPLWQTMIVMTMFG
                     VGLGFNFQPLTLAVQNAVPPQQIGVATSTATFTRQIGGTLGTAVFLSILFSTASEKIT
                     TALAAAVPTQEFQAALADPANAAVAEQFAAAQSGGGAATSVLSDSSFLTGLDERLAKP
                     FLIGFSEAMDLVFLTGSAVMVIGFLVMLMLPNVELRKSSGYSERSIAE"
     gene            complement(4144..4620)
                     /locus_tag="PL014_09105"
     CDS             complement(4144..4620)
                     /locus_tag="PL014_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT57289.1"
                     /translation="MPFTSPRSSPTAILGDDLITVVKLLHLVRQHAPRQHPQVDPMAY
                     PLLFNLVAAPKRVSALAEAIHSDVSTVSRQVSTLVDLGFIDRGPDPDDRRAQALTLTD
                     EGRTLLLAIRDDRDRWMQGLLADWDPDDVAQFSTHLRHFAKDLENSLTALTSRTTS"
     gene            complement(4721..5194)
                     /locus_tag="PL014_09110"
     CDS             complement(4721..5194)
                     /locus_tag="PL014_09110"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT57290.1"
                     /translation="MATFTTSNRSVADVTADVNDVWEVLTDPDLLARLTPFLHSVTEH
                     GEHWVWQLTKVPVLGTSFSFTFRELMAFDEPHRINFTHDPAPGAAETAGVVGYYALTP
                     HPRGTHLETSMTITVDLPFPGLVRPGVTAAMRGVIAIMGQRFGHNLLAHLGATNA"
     gene            complement(5206..6054)
                     /locus_tag="PL014_09115"
     CDS             complement(5206..6054)
                     /locus_tag="PL014_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HOT57291.1"
                     /translation="MTLHPPAPSDLGELVDAYAQTVRNVIDVGRTTRPGDESLPTDCP
                     GWSVLDQFAHIASTEAMAAGEPLPEVDVSRHGHVRHAFGERIERYLESRRGRSLEEVL
                     DELEERRDERLALYRSGTAWAQTPVAGPFGPTTLGALLGIRVFDIWMHEQDIREALGR
                     PGGLESGAASHTVAQLFAALPRIVAKTAAIEPGNAVVLDLTGPTTGRAGVRVEEQDGR
                     AVGVPLFTGGAEDHGDAVTTSLTMSTQVATRLAGGRRRPQDAHVVVHGDDAVAQRVLA
                     ALTITP"
     gene            6082..>7806
                     /locus_tag="PL014_09120"
     CDS             6082..>7806
                     /locus_tag="PL014_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOT57292.1"
                     /translation="MPTREDTAIDTRSSAAQHGPAGPYGALGLAVAAGLGAVVVAALI
                     TGAVAPLDLGDPGPLVRWGVPVVSTISTLAASATVGLLGLAAFLMPERSRTSRRVTAT
                     RYAAGAATIWAIAALLEVVLAFADLAGTPLTSPELLDQLVSFVWTLETTRVLLISALV
                     AVVVAACARLATRRAPMAWLAVFTLFAVVILALTGHAAGSASHEDAVNALAIHLLGVV
                     VWTGGLIGLIVMRRGLGSDLGPTVSRYSTVAAWCFAAVTVTGIQQAWIRLGSFDAITT
                     SYGAVVAMKTVAIIALGVLGWLHRRTIATRLTANPGDGSAFARLAVVEIAVMGAATGL
                     AAVLARSIPPVPDAPPAPSRILELTGYPDPGPMSSGDWFTAWRIDWLFLAVAVLAVGL
                     YLAGVVRLRRRGDAWPVLRTVCWVLGWALFVWATCGAPGIWGRVLFSVHMVMHMAVAM
                     IVPLLLVPGAPVTLALRTLKARPDKTWGPREVLLQVVRSRAMKVLANPVVAACFFFFS
                     LAAFYWTGLFELSLTTHTGHLLMIAHFLLTGYLFTWVLIGIDPGVPRWNPLMLLVILF
                     ATISFHAFF"
BASE COUNT         1151 a         2617 c         2879 g         1159 t
ORIGIN      
        1 ccgacttcgt gcgacttcgg tgcggatttc tgggctgaat cgcgccgggg tcgcacgcaa
       61 gtcgcacgaa gtcgggtcgg gggtcgggtg tgagggtcgg aggaggaggt cggatgccgg
      121 tcagctcgcc gcgtcgtcgg cgtcgtcctc acggccgggt gtgcgcaggt tccaccggcg
      181 gatgacgaag cggaacagca cgtagtagac caccgcgtac cccaacccca tgacgatgag
      241 cagggccggt ctcgtggcga tgttccagtt cagcaggaag tcgaacagcc ctgcgctgaa
      301 gccgaacccg tcccggatct ccaaggcgtt cgccagcgcc atcgacgtgc cggtgagcac
      361 ggcgtgcacg acgtacagcg gccaggcgac gaacatgaag gcgaactcga tgggttcggt
      421 caccccggtg aggaacgccg tgagcgccac cgacgccatc agcccaccga cggccttctt
      481 cgccttgggc ttggcctcct ggtagatcgc gagggcggca gcgggcaggg cgaacatcat
      541 gatggggaag aacccgctca tgaacgcccc ggcggtcggg tcgcccttga ggaagcgcgc
      601 gatgtcgccc tgcacggtgt cgccaccggc gggcgtgtac tcgccgagca cgaaccacgg
      661 caccgagttg aggatgtggt gcaggccgag cgggatgagg aggcggttgg cggtgccgta
      721 gatgaagccg ccgatgatcg agttcgcggc gacccactca ccgaggccgg tcagcacggc
      781 gtcgaacgcc gggtagatga tcgcgagcag caccgcgagc acgagggtcg cgaagccggt
      841 gacgatcggc acgaaccggc gaccaccgaa gaaggccagg tagggcggca gcgagatccg
      901 gtagtagcgc tgccacagca cggcggcgat gagcccgcac aggatgccgc cgagcacgcc
      961 gtagttgacc agttcctggt cctcacccgg tttgggggcc gggaggatga agggcgacac
     1021 ggcatccccg acgcccttga agacgaggta accgacgacc gcggccaggg ccgtcgaccc
     1081 gtcggccttc ttcgcgacgc cgatggccac gccgacggcg aacagcaggg gcaggttcgc
     1141 gaacagggcc ccgccgccgg caccgacgac ggcggcgacc ttctcccagc ccaggccgtc
     1201 gcgcccgagc aggtcgggtt ggcccaggcg cagcatgagg gcggcgaccg gaagggccgc
     1261 gatcggcaac atgaggctgc ggccgaactt ctgcagcggc gccaggttga ggcgcttctt
     1321 gtccgggccg gcagcggcga cgtcagccgt ggtcatggcg gtggagcctc ctcaaaggca
     1381 agtcggtgag aggctgactc catcacaccg gcgcgacgaa tgcccgaaag gaccctcccg
     1441 gcatgagcag caaggcccag cagatcctga tcgccctcgg cggcgcggac aacatcatcg
     1501 agatcgagcc gtgcatcacc cggctgcgca ccgaggtcaa ggacccgacc gtcgtcgacg
     1561 aggccgcgct caaggaggcc ggtgcccacg gtgtcatccg cgccgggtcg gtcgtccagg
     1621 tcgtggtcgg gcccgaggcc gacaacctcg ccgaggacat cgaggacctg atgtgagccg
     1681 accgcaggct ctcctgctcg acctcgacgg caccctcgtg gactccgagc ccatccaccg
     1741 ggcggggttc gagcggtggt tcgcccaccg cggctgggcg ttcgaccgcg aggtggcctc
     1801 gctgttcacc ggccgacgtg ccgacgacgt cttccgcacc gtcgacgggc cgtggagcgg
     1861 cggggacccg acggagatgt tccacgagat cgtcgccatg gtgccccgcg accgactgcc
     1921 ggatgccgtg ccgggcgcgg tggcgagcat cgagcgggcg ctcgcggccg gcatcccact
     1981 ggcgctcgtc acgtcagccg gggccggtgg gccgacaagg cgctggccgc gctcgggggc
     2041 atcgaccggt tcgacgtcgt cgtcacccgc gacgacgtca ccgtgggcaa gcccgacccg
     2101 tcggggtacg ccctcgcctg cgaacgactc ggggtggatg ccgcccgcgc cctcgcgtgc
     2161 gaggacgcac ctcagggggt ggtcgccgcc gtctctgccg gagtgggaac cgtcgtcggg
     2221 gtgacgacga gcttctcgca ggaggtgctg cgcgaggccg gggcgcacgc gacgatgccg
     2281 gacctgaccg gcatcccgac cctcctcggc ccgggccacc cctgacccga tccgacgtct
     2341 tgcactccgg ccactgccac tgaccagctg gccacagcca gtcgagagga ggcaacacgc
     2401 agatcaggcg tctcggccct cggcgtgttg cctcctctcg agttggcacc gggcgggaca
     2461 cccggcgccg gcggtgtcac tcggcgatgg agcgctcgct gtagcccgag ctcttgcgca
     2521 gctcgacgtt gggcagcatc agcatgacga ggaagccgat caccatcacg gccgacccgg
     2581 tgaggaagac caggtccatc gcctcggaga acccgatgag gaacggcttg gccaggcgct
     2641 cgtcgaggcc ggtgaggaac gagctgtcgg acagcacgct cgtcgccgca ccaccgccgg
     2701 actgtgccgc cgcgaactgc tcggcgaccg cagcattggc cgggtcggcc agtgcggcct
     2761 ggaactcctg cgtcggcact gctgcggcca gcgcggtcgt gatcttctcc gacgcggtcg
     2821 agaacaggat cgacaggaac acggcggtgc cgagcgtgcc accgatctgg cgggtgaacg
     2881 tggccgtcga ggtggcgacg ccgatctgct gcggcggcac ggcgttctgc acggccaggg
     2941 tgagcggctg gaagttgaag cccagcccca cgccgaacat cgtcatgacg atcatcgtct
     3001 gccacagcgg ggtgtcatag gcgacgtagt ggaagatgaa cagcgagatc gtcagcaggg
     3061 ccgagccgac gatggggaag acccggtagc ggccggtgcg cgagatgatc tggccggaca
     3121 ggatcgagcc gatcatgatg ccgagggtca tcggcagcag cagcagcccg gcctcggtgg
     3181 gcgtcgcacc cttgacgatc tgcaggtaga gcggcagggc ggccaggcca ccgaacatcg
     3241 cgacaccgat gagcaccgag gcgatcgagg acaccccgac ggtcttgttg cggaacatcg
     3301 cgagcggcag gatcgcctcc tcgcccatgc ggtgctcctg ccagaagaag ccgatggcgg
     3361 cgacgacacc gatgacgtag cagatgatcg cgttcgtgga gccccagccc cactcgcggc
     3421 cctgctcggc gaccagcagc agcggcacga gcgagagcga gagcagcgcg gccccggccc
     3481 agtcgatgcg gtgatccagg cgggtgtggc gcaggtgcag cgtcttggtc acgacggcga
     3541 gggccgcgat gccgatcggc acgttgacga ggaagaccca gcgccagccg tcgatgccca
     3601 ggatgctgct ctggccggcg aagaacccgc cgatgaccgg gccgagcacg gatgccgtgc
     3661 cgaacaccgc gaggaagtag ccctggtact tggcgcgctc gcgcggggga acgatgtcgc
     3721 cgatgatcgc cagcgccagc gagaacaggc cgccggcgcc cagaccctgc accgcgcggt
     3781 agaacgcgag cgagcccatc gaggtggcga acgtgcacag cacggacccg aggatgaaca
     3841 gcgagatggc gatgatgaac agcttcttgc ggccgtagat gtcgccgagc ttgccgtaga
     3901 gcggcgtgac gatcgtcgag gtgatgaggt aggcggtggt cgcccaggcc tggtggttca
     3961 ggccctggag gtcgtcggcg atggtccgga tggccgtggc gacgatggtc tggtcgagcg
     4021 cggcgaggaa catgcccatc atcaggccga tgaggatggt gacgatctgc cggtggctca
     4081 gcggcccggc cggggcatcg accgccgtgg cggacgccgc gtcagcggcg ggggaggagg
     4141 tgctcatgac gtggtccttg aggtgagggc ggtcagggag ttttcgaggt ccttggcgaa
     4201 gtggcgcagg tgggtcgaga actgggcgac gtcgtcgggg tcccagtcgg cgagcaggcc
     4261 ctgcatccag cggtcgcggt cgtcgcggat ggcgaggagc agggtgcggc cctcatcagt
     4321 gagggtgagg gcctgggcgc ggcggtcgtc cgggtcgggc ccacggtcga tgaaaccgag
     4381 gtcgaccagg gtgctgacct ggcggctcac ggtcgagacg tccgagtgga tcgcctctgc
     4441 gagggccgac acccgcttcg gcgcagcgac gaggttgaac aacagcgggt aggccatcgg
     4501 gtcgacctgc gggtgctgtc gtggtgcgtg ctggcgaacg aggtgcagca gcttcaccac
     4561 cgtgatgagg tcgtcgccca ggatggccgt gggcgaggac cgcggtgacg tgaacggcat
     4621 ccgatcaccc tccgtggata gttgcatgag tcaagcaact atgcgcggtc gtgccgcatt
     4681 cccgcgtggt gcacgtgttt tcggatgccg gggagctggg tcaggcgttc gtcgcgccga
     4741 ggtgggcgag caggttgtgg ccgaagcgct gacccatgat cgcgatgact ccgcgcatcg
     4801 ccgcggtcac ccccgggcgc acgagcccgg ggaagggcag gtcgacggtg atggtcatcg
     4861 acgtctcgag gtgggtgccc ctggggtgcg gggtcagcgc gtagtagccg acgacacccg
     4921 ccgtctcggc ggcacccggg gccgggtcgt gggtgaagtt gatgcggtgg ggctcgtcga
     4981 aggccatgag ctcacggaag gtgaacgaga agctcgtgcc gagcacgggc accttggtca
     5041 gctgccacac ccagtgctcg ccgtgctcgg tgaccgagtg caggaacggg gtgaggcgtg
     5101 ccagcaggtc ggggtcggtg agcacctccc acacgtcgtt gacgtccgcg gtgacgtctg
     5161 ccaccgagcg gttcgaggtc gtgaaggtcg ccatggtccc ggccctcagg gggtgatcgt
     5221 cagggcggcc aggacgcgct gcgcgacggc gtcgtcaccg tggaccacga catgcgcatc
     5281 ctgaggcctg cgccggccgc cggcgaggcg ggtcgcgacc tgggtcgaca tcgtcagcga
     5341 cgtcgtcacg gcatccccgt ggtcctcggc gccgccggtg aacaggggga caccgacggc
     5401 gcggccgtcc tgttcctcga cgcgaacccc ggcgcgcccg gtcgtcgggc cggtgaggtc
     5461 gagcacgacc gcgttccccg gctcgatggc agccgtcttc gcgacgatgc ggggcagggc
     5521 cgcgaacagc tgcgccacgg tgtgcgacgc agcccccgac tccagcccgc ccggccgccc
     5581 gagcgcctcg cggatgtcct gttcgtgcat ccagatgtcg aagacgcgga tgccgagcag
     5641 cgccccgagc gtcgtggggc cgaacgggcc ggccacgggg gtctgcgccc aggcggtgcc
     5701 agaccggtag agcgccaggc gctcgtcacg ccgctcctcg agctcgtcga gcacctcttc
     5761 gaggctgcgc ccgcgccgtg actcgagata gcgctcgatg cgctcgccga aggcgtgccg
     5821 cacgtggccg tgccggctga cgtcgacctc cggcaggggt tcgcccgccg ccatcgcctc
     5881 ggtgctcgcg atgtgggcga actggtcgag cacgctccag ccggggcagt cggtgggcag
     5941 cgactcgtcg ccggggcggg tcgttctacc gacgtcgatg acgttgcgca ccgtctgggc
     6001 gtaggcgtcg acgagctcgc cgaggtcgct gggcgccggt gggtgcaggg tcatccggcc
     6061 acagtaccta cgctggtgcc catgcccacc cgagaggaca ccgcgatcga cacccgctcg
     6121 tcggcggctc agcacggccc ggcagggccg tacggggctc tcgggctcgc cgtcgcagcc
     6181 ggtctggggg ccgtggtcgt ggccgcgctg atcaccggcg ccgtggcgcc gctcgacctc
     6241 ggcgaccccg gcccgctcgt gcgctggggg gtgccggtgg tctcgacgat cagcaccctc
     6301 gcggccagcg caaccgtcgg gctgctcggg ttggcggcct tcctcatgcc cgagcggtcg
     6361 cgcacgagcc gccgggtcac cgccacccgg tacgccgccg gcgcggccac gatctgggcg
     6421 atcgccgcgc tgctcgaggt cgtgctcgcg ttcgccgacc tcgccggcac gcccctgacc
     6481 agccccgagc tgctcgacca gctcgtctcg ttcgtctgga ccctcgagac cacccgggtc
     6541 ctgctcatca gcgccctcgt ggccgtggtc gtcgcggcgt gtgcccggct ggccacccgc
     6601 cgtgctccga tggcctggct cgcggtgttc accctgttcg ccgtcgtcat cctcgcgctg
     6661 acgggtcacg ccgccggatc ggcgagccat gaggatgccg tgaacgcgtt ggcgatccac
     6721 ctcctcggcg tcgtggtgtg gaccgggggg ttgatcggtc tcatcgtcat gcgccgcggc
     6781 ctcgggtcag acctcggccc cacggtgtcg cgctactcga cggtcgcggc ctggtgtttc
     6841 gccgcggtga ccgtgaccgg catccaacag gcgtggatcc ggctcgggtc gttcgatgcc
     6901 atcaccacgt cctacggcgc ggtggtcgcg atgaagaccg tggcgatcat cgcgctcggc
     6961 gtgctgggct ggttgcaccg gcgcacgatc gcgacccggc tgacggccaa ccccggtgac
     7021 ggcagcgcgt tcgcccggct cgccgtcgtc gagatcgccg tcatgggcgc cgccaccggg
     7081 ctcgccgcgg tgctcgcgcg cagcatcccg ccggtgcccg acgccccgcc tgctccgagc
     7141 cgcatcctcg agctgaccgg gtacccggac ccggggccga tgtcgtcggg tgactggttc
     7201 accgcgtggc ggatcgactg gctgttcctc gccgtcgcgg tcctggccgt cgggctctac
     7261 ctggcggggg tcgtgcggtt gcggcggcgc ggcgacgcct ggccggtgct gcgcacggtg
     7321 tgctgggtgc tcgggtgggc gctgttcgtg tgggccacgt gtggggcgcc cggtatctgg
     7381 gggagggtgt tgttcagcgt gcacatggtg atgcacatgg ccgtcgcgat gatcgtgccg
     7441 ctgctgctcg tgcccggtgc gccggtgacc ctggcgctgc gcaccctcaa ggctcgcccc
     7501 gacaagacgt gggggccgcg tgaggtgctg ctccaggtgg tgcgctcccg ggcgatgaag
     7561 gtgctggcga accccgtggt cgcggcgtgc ttcttcttct tcagcctggc cgccttctac
     7621 tggaccgggc tgttcgagct ctcgttgacc acccacaccg ggcacctgct gatgatcgcc
     7681 cacttcctgc tcaccgggta cctgttcacc tgggtgctca tcggcatcga ccccggggtc
     7741 ccgcgctgga acccgctcat gctgctcgtc atcctcttcg cgacgatctc cttccacgcc
     7801 ttcttc
//
LOCUS       DAOSOC010000115         7802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9315_length_7802_cov_9.298180, whole genome shotgun sequence.
ACCESSION   DAOSOC010000115 DAOSOC010000000
VERSION     DAOSOC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9315_length_7802_cov_9.298180"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PL014_09125"
     CDS             complement(<1..316)
                     /locus_tag="PL014_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT57293.1"
                     /translation="MTTTDAVRTGDLPAAGWVPPTWEEVVEQHSARVYRLAYRLTGNV
                     HDAEDLTQDVFVRVFRSLHTYQPGTFEGWLHRITTNVFLDKMRRKQRIRFDALSDESA
                     ARL"
     gene            454..1089
                     /locus_tag="PL014_09130"
     CDS             454..1089
                     /locus_tag="PL014_09130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOT57294.1"
                     /translation="MSTLRPASWTYTEEFVTEPEVIENARRRGLEFGDASPVGTGAGA
                     MLRVVAAAVQARHVVEIGTGAGTSGLWLLSGMPDDGVLTTIDSSAEHQRAAREAYAEA
                     GYAHQRTRVITGDAAEVLPRMTDGAYDLVVIDADKPGYPTYVEHAIRLLRPGGVLVMD
                     NMLWHDQVADPAARDATTSTLRDLGKALRDHDQLTTTLLPVSDGLLVAVKR"
     gene            complement(1099..1455)
                     /locus_tag="PL014_09135"
     CDS             complement(1099..1455)
                     /locus_tag="PL014_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57295.1"
                     /translation="MNAGYGLTVRWSLEDAPADAADQLREYVVGTSIARFMFLDGLAF
                     KVWRMREGEWFEGTYVFDSEQDRTVFREGFVPGAATAPGSVMIGSAPITIEDWEVVAI
                     AEGPAGFRRGAGPEVS"
     gene            complement(1452..2987)
                     /locus_tag="PL014_09140"
     CDS             complement(1452..2987)
                     /locus_tag="PL014_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HOT57296.1"
                     /translation="MPRVDVLRHPATSSWSVLPDLGAALAAIQSGGSDPDGTALVAVV
                     GADDALGDTSARAAIEALGLHADDVLTTFAPSTKAGAQTTIPLMPGAHAVRRVVLVGV
                     DDGSDAAWRSAGAAIGRATRGRSRVVHAVAGAAGSQALAAYVEAVVLASWSSPRWTRK
                     GPTVVTHPASEAVVAGTEDAGTVADAEARARAQLLARGLSMTPSNTKNPAWLARQARA
                     VAKQSHLDVRVWDERQLAREGFGGLIAVGSGSATPPRLVQLDHVPDGATAATPRVVLV
                     GKGITFDTGGLQVKPVDGMVGMKTDMSGAAIVLAVVAACHELGVAVRVTGLLALAENA
                     VDGASYRPGDVVTQYGGRTVEIGNTDAEGRIVLADALAYADAALDPTVLVDVATLTGA
                     ARIALGRTIAPVFATDAALGAALEAAGATTGETLWPMPLAQTYRRALDSDVADVAHIA
                     PGVGGGAVTAALFLREFVGERPWAHLDIAGTGRSDVDAGTSAKGATGFGTRLLLRWLE
                     EMA"
     gene            complement(3078..3251)
                     /locus_tag="PL014_09145"
     CDS             complement(3078..3251)
                     /locus_tag="PL014_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HOT57297.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVLRMPLEGGGRLVVEMTPDEVR
                     ALGAAIDNCEGLA"
     gene            complement(3387..4682)
                     /locus_tag="PL014_09150"
     CDS             complement(3387..4682)
                     /locus_tag="PL014_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57298.1"
                     /translation="MALVDRAPAQPVRPSLLTSEGRHALGARALNHPVLTILAVYLIS
                     RLVALLALWVAATWFQTPAGVGHDDPGLGDMFGLWDGVWYQRIAQDGYPVPLPVDADT
                     GRVTYSAWAFYPLYPYLVKLLMVTGMPFTAAGVVLNVVLGGVAALLIWAVLRSGEHAD
                     RQPQRDRLALVAAGLWCFYPTTAVMLKPYTEALAVALVAGALLMLIRRNYLLVALVAI
                     PLGFARGVAPALGVAVLIHLVVRWREDRAAGVPPLLGQRWRALVMLAAVGVSGVAWPV
                     VAAVATGVPTAFFDVQAAWGQRPDKGPFVLWVEWAWQGKGVAGVVVLVALLGTYVALV
                     LGRHGRWICIEARAWALAYPLYMFAVVRPITSMWRFLLLDFPIAALVASVAMRTANGE
                     RVVPHWRRRVAVVLVVVVAGLFWWTAALLVYTPWGSFPP"
     gene            complement(4669..6687)
                     /locus_tag="PL014_09155"
     CDS             complement(4669..6687)
                     /locus_tag="PL014_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57299.1"
                     /translation="MTAPLDDALRTQLRGYRREQVDEVIRVLEGRVAAHDRAIAHLRG
                     EAHDPMFEHPDEAPGLLGPRVTAAALAPDDASQTQPGRAGNTSRDRSTTEPSPLPWRR
                     SDLAAPAVYLLVAVWLLSGLVADLRTGYLSQGVQDQQAFEWYFGATAHNLVTFSNPLF
                     TDLQNFPDGVNLMANAAVLGLGVPLAPLTLLAGPSATFVLVEVLGLALTASAWFWLFR
                     RRLAVHPLLAAIGAGFVGFAPGMVSHANGHPNFVAQFLVPVIVDRVIRLAQGGRPRRD
                     AVVLGLLAAWQVFIGEEVLLLTAIGLAIAAPVVLAHHREVLRPLARGLALGAGVCLAI
                     VAVPLWWQFAGPRSYTSIWHPPGGNDLAQLWGRATRSIGADVWASAALSMNRTEENSF
                     FGIPLLLAAVAVVIALWRRPAVQALGVVVLISCWFSLGEEVVVNGRSTGIPAPGVLFE
                     HVPILENVLPTRFALIAVPALGALLAIGAEEVRRAVGRSSASPGAALAAAAGAAGLVL
                     IPVLPTPLVVDQRAPMPQFFVDGSWKDWVDEGGSVLAAPAPWVGDTRALEWQAQARWG
                     FPVVAGYFVGPDTTPERGGQYGATPTGLTQWMAAIAEADQPTLATPEQVEEFTTNLRQ
                     GRVDAIVLPQGRPAEGALLASLSSAFGEPVTTGGVHVWDVRSVTDGAG"
     gene            complement(6684..7115)
                     /locus_tag="PL014_09160"
     CDS             complement(6684..7115)
                     /locus_tag="PL014_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57300.1"
                     /translation="MACTITRRTPLAPAVAFAALTDLAEHTAHVPLTDVEVPEQGLAL
                     GAEVIAWTRLGPLAIADRMLVTALEPGHRLRLVKTGRFLHGWADITVRPDPERPGGSS
                     IVWAEELWLPGLKTLTRPIGDRLAAVLFARVVDGVLARAAA"
     gene            complement(7120..7419)
                     /locus_tag="PL014_09165"
     CDS             complement(7120..7419)
                     /locus_tag="PL014_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT57301.1"
                     /translation="MVALIVLGLVAVALVLVVLGVATGRVPADPLVAAVSTTPETGLP
                     EVPSASDVAAVRFDTEVRGYRIDDVDTRLEVLHETLAQREAELARLLDENRSGGQ"
     gene            complement(7449..>7802)
                     /locus_tag="PL014_09170"
     CDS             complement(7449..>7802)
                     /locus_tag="PL014_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOT57302.1"
                     /translation="VNFRYFFARKTMFVKYAQGFIVLPGGFGTLDELFEAVTLVQTQK
                     VTSFPIVLLGRDYWGGLLDWLRDTAAARGTISTGDLDLLTLTDDVDEAVGTIIAADAS
                     ARRSSTPSPPAGTSE"
BASE COUNT         1203 a         2783 c         2874 g          942 t
ORIGIN      
        1 cgaggcgggc ggccgactcg tcggagaggg cgtcgaagcg gatccgctgc ttgcggcgca
       61 tcttgtcgag gaagacgttg gtcgtgatgc ggtgcagcca gccctcgaac gtgccgggct
      121 ggtaggtgtg cagcgaccgg aacacccgca cgaacacgtc ctgggtgagg tcctcggcgt
      181 cgtggacgtt ccccgtgagg cggtacgcga gccggtacac ccgcgcggag tgctgctcga
      241 cgacctcctc ccacgtcggg ggaacccaac cggcagccgg caggtcgccg gtccgcacgg
      301 catccgtcgt cgtcatcgtc tcttccctca ccgagctgct catgtcaccc atcgatacgc
      361 gtccagtccg acaattgttc ccggattccc tgaacaaacg ctgggaacgt gcgtcggggc
      421 cttcgaccgc gccccgggcc atagggttga cgcatgagca ccctgaggcc cgccagctgg
      481 acgtacaccg aggagttcgt gaccgagccg gaggtcatcg agaacgcccg acggcgcggt
      541 ctggagttcg gcgacgcctc cccggtcggc acgggcgccg gggcgatgct gcgcgtcgtc
      601 gcggccgctg tccaggcgcg gcacgtcgtc gagatcggca cgggggccgg cacgtcgggc
      661 ctgtggctgc tctccggcat gcccgacgac ggggtcctca cgaccatcga ctcctcggcc
      721 gagcaccagc gagccgcccg cgaggcctac gccgaggccg gctacgccca ccagcgcacc
      781 cgcgtcatca ccggggacgc cgccgaggtg ctgccccgga tgaccgacgg cgcctacgac
      841 ctggtcgtca tcgacgccga caagcccggc tacccgacgt acgtcgagca cgcgatccgg
      901 ctgctgcgac cgggcggggt gctcgtgatg gacaacatgc tctggcacga ccaggtcgcc
      961 gaccccgcgg cgcgcgacgc gacgacgagc accctgcgcg acctcggcaa ggcgctgcgc
     1021 gaccacgacc agctcacgac gaccctgctg ccggtgagcg acggcctgct cgtcgccgtc
     1081 aagcgctgag gcgaccgctc agctgacctc ggggcccgcc ccgcggcgga acccggcggg
     1141 gccctcggcg atcgcgacga cctcccagtc ctcgatggtg atcggcgccg agccgatcat
     1201 cacgctcccc ggtgccgtgg cggcaccggg gacgaagccc tcgcggaaga ccgtgcggtc
     1261 ctgctcggag tcgaagacgt acgtcccctc gaaccactcg ccctcgcgca tgcgccacac
     1321 cttgaaggcg aggccgtcga ggaacatgaa ccgtgcgatc gaggtgccga cgacgtactc
     1381 gcgcagctgg tcggcggcat ccgccggggc gtcttccagc gaccagcgaa cggtcagccc
     1441 gtaaccggcg ttcatgccat ctcctccagc caccgcagca gcagtcgtgt cccgaaaccg
     1501 gtggccccct tggcggaggt gccggcgtcg acgtccgagc ggccggtgcc tgcgatgtcg
     1561 aggtgtgccc acgggcgctc accgacgaac tcccgaagga acagggccgc cgtcaccgca
     1621 ccgccaccca cgccgggagc gatgtgggcg acgtcggcga cgtcggagtc gagggctcga
     1681 cggtaggtct gcgcgagggg catcggccac agcgtctcac cagtcgtcgc cccggcggcc
     1741 tcgagcgcgg cgcccaaggc cgcgtcggtg gcgaagaccg gtgcgatggt tcgccccagc
     1801 gcgatgcgcg cggcaccggt gagggtcgcg acgtcgacca ggaccgtggg atcgagtgcg
     1861 gcatcggcgt aggcgagtgc gtcggcgagc acgatgcggc cctcggcgtc ggtgttgccg
     1921 atctcgacgg tgcgaccgcc gtactgggtg acgacgtccc ccggccggta cgaggccccg
     1981 tcgacggcgt tctccgccag cgcgagcaga ccggtgaccc gcaccgcgac cccgagctcg
     2041 tggcaggcgg ccacgacggc caggacgatg gccgctccgg acatgtcggt cttcatcccg
     2101 accatgccgt cgaccggttt gacctggagg ccaccggtgt cgaacgtgat gcccttgccc
     2161 accagcacga cacgtggggt cgcagcggtg gcgccgtccg ggacgtggtc gagctggacg
     2221 agacgaggcg gcgtggccga ccccgacccc acggcgatca ggccgccgaa cccctcccgg
     2281 gcgagctggc gctcgtccca gacccgcacg tcgaggtggc tctgcttcgc cacggcccgg
     2341 gcctgccggg ccaaccaggc cgggttcttg gtgttcgacg gtgtcatcga caggcctcgc
     2401 gcgagcagct gggcgcgggc ccgggcctcg gcgtcggcga ccgtcccggc atcctcggtg
     2461 ccggcgacca cggcctcgga tgccgggtgc gtcaccaccg tgggtccctt gcgggtccag
     2521 cggggagagg accaggacgc gagcacgacg gcctcgacgt aggccgcgag ggcctgcgag
     2581 cccgcagccc ccgccacggc gtggaccacc cgcgatcggc cgcgagtcgc gcggccgatc
     2641 gcggcgccgg cactgcgcca cgcggcatcc gagccgtcgt cgacgcccac gaggacgacc
     2701 ctgcgcacgg cgtgcgcacc gggcatcagg gggatggtcg tctgcgcccc cgccttggtg
     2761 ctgggcgcga acgtcgtgag gacgtcatcg gcatggaggc cgagcgcctc gatggcggcg
     2821 cgggcgctcg tgtccccgag cgcgtcatcg gcaccgacga ccgcgacgag ggctgtgccg
     2881 tccgggtcgc tgccgccgga ctggatggcc gcgagggctg ccccgaggtc gggcagcacg
     2941 ctccacgagg aggtggctgg gtggcgcagg acgtcgactc ggggcaccgg tcgaccatat
     3001 cgagcgggat gcctgacacg cgccgaggcc cccaccggtt tcggtggggg cctcggcgtg
     3061 gaggggtcag gcgtgggtca cgccagacct tcgcagttgt cgatcgcggc gcccagggcg
     3121 cgcacctcgt cgggagtcat ctcgacgacg agtcgccccc caccctccag aggcatgcga
     3181 aggacgatgc cacgaccttc cttggtgacc tcgagcgggc cgtcgcccgt cctcggcttc
     3241 attgccgcca tggctcaaat cccttcctcg ccgcggcgcc gtctggtgcg tgccgcgtgg
     3301 tgtccgacgc cggagggccg gaatgctgac ccattatccc gcagtggtgc ggcgcgacga
     3361 aatccggctc aacgcacggc ggtcgctcac ggagggaagg agccccacgg ggtgtacacc
     3421 agaagggcag ccgtccacca gaagagcccg gcgacgacga cgacgaggac gaccgcgacc
     3481 ctgcggcgcc agtggggcac gacgcgttcg ccgttcgccg tgcgcatcgc gaccgaggcc
     3541 accagggcgg cgatcgggaa gtcgaggagg aggaagcgcc acatgctcgt gatggggcgc
     3601 acgaccgcga acatgtacag cgggtaggcg agcgcccagg cgcgggcctc gatgcagatc
     3661 caccggccgt ggcgaccgag cacgagggcc acgtacgtgc cgagcagggc gacgaggacg
     3721 acgactcccg cgacaccctt gccctgccag gcccactcca cccagagcac gaacggcccc
     3781 ttgtcggggc gctgacccca cgccgcctgc acgtcgaaga acgccgtcgg gacccccgtg
     3841 gccaccgcgg ccacgacggg ccaggccaca ccgctcaccc cgaccgccgc gagcatgacg
     3901 agggcacgcc accgctgccc caggagaggg gggacgccgg ccgcccggtc ctcgcgccag
     3961 cgcaccacga ggtggatgag gacggccacc ccgagcgcgg gggccactcc ccgggcaaac
     4021 cccaggggga tggcgaccag ggccacgagg aggtagttgc gccggatgag catgagcagg
     4081 gccccggcga ccagcgccac ggccaaggcc tcggtgtacg gcttgagcat gacggcggtc
     4141 gtcgggtaga agcaccagag gccggcggcc acgagggcca gccggtcgcg ctggggctga
     4201 cggtcagcgt gctcacccga gcgcaggacc gcccagatga gcagggcggc gacaccaccg
     4261 aggacgacgt tgagcaccac cccggcggcg gtgaacggca tccccgtcac catgagcagc
     4321 ttcaccagat aggggtagag cgggtagaac gcccaggcgc tgtacgtcac gcggccggtg
     4381 tcggcatcga ccgggagcgg gacggggtag ccgtcctgcg cgatccgctg gtaccagacg
     4441 ccgtcccaca gcccgaacat gtccccgagg ccggggtcgt cgtggccgac cccggccggg
     4501 gtctggaacc acgtcgccgc gacccagagc gcgagcagcg caacgaggcg ggagatgagg
     4561 tagacggcga ggatggtgag caccgggtgg ttcagggcac gcgcgccgag ggcgtgcctg
     4621 ccctcggagg tcagcaggct cggccgcacg ggctgtgccg gggctcggtc aaccagcgcc
     4681 atcggtcacg ctccggacgt cccagacgtg gacgccaccc gtcgtcacgg gctcgccgaa
     4741 ggcactggag agcgaggcca gcagggcccc ctcggccggc cggccctgcg ggagcacgat
     4801 ggcatcgact cggccctgcc gcaggttggt ggtgaactcc tcgacctgtt cgggggtcgc
     4861 gagcgtcggc tggtcggcct cggcgatcgc cgccatccac tgggtcaggc ccgtcggggt
     4921 ggccccgtac tggccccctc gttccggggt ggtgtcgggc ccgacgaagt acccggccac
     4981 gacggggaaa ccccatcgcg cctgcgcctg ccactccagg gcccgggtgt cgccgaccca
     5041 gggggccggc gccgcgagga cggacccgcc ctcgtcgacc cagtccttcc acgacccgtc
     5101 gacgaagaac tgcggcatcg gggcccgttg gtcgacgacg aggggcgtcg ggagcacggg
     5161 gatcaggacg agacccgccg cgcctgctgc ggcggccagg gcagcgcccg ggctcgccga
     5221 ggaccggccg accgcgcgcc tcacctcttc ggcaccgatc gcgagcagcg cacccagcgc
     5281 cggcaccgcg atcagggcaa agcgggtcgg gagcacgttc tcgaggatcg ggacgtgctc
     5341 gaacagcacg ccgggtgcgg ggatgccggt ggatcgaccg ttgacgacga cctcctcacc
     5401 gagtgagaac cagcaggaga tgagcacgac gactcccagc gcctggacgg ccgggcgccg
     5461 ccacagcgcg atgacgacgg cgaccgcggc aagcagcagg gggatgccga agaaggagtt
     5521 ctcctcggtg cggttcatcg acagcgcggc cgacgcccac acgtccgcgc cgatgctgcg
     5581 ggtggcccga ccccacagct gcgcgaggtc gttgccgccc ggcgggtgcc agatggaggt
     5641 gtagctgcgt ggaccggcga actgccacca cagcggcacc gcgacgatgg ccaggcagac
     5701 gccggcgccg agagccaggc cccgcgcgag cgggcgcagc acctcgcggt ggtgggcgag
     5761 caccaccggg gcggcgatcg ccaggccgat ggccgtgagc agcagcacct cctcgccgat
     5821 gaacacctgc caggccgcga gcaggccgag gaccaccgcg tcccgtcggg gacgcccacc
     5881 ctgcgcgagg cggatgaccc ggtcgacgat gaccggcacc aggaactggg cgacgaagtt
     5941 ggggtggccg ttggcgtgcg agaccatccc cggtgcgaag ccgacgaaac cggcgccgat
     6001 ggccgcgagc agggggtgga ccgcgagacg acggcggaac agccagaacc aggcggatgc
     6061 cgtgagcgcc agccccagca cctcgacgag cacgaacgtg gccgacggtc ccgcgagcag
     6121 ggtcaggggg gccagcggaa cgccgagccc gagcactgcc gcgttggcca tgaggttgac
     6181 gccgtcgggg aagttctgga ggtcggtgaa cagggggttg gagaacgtga cgaggttgtg
     6241 ggcggtggcc ccgaagtacc actcgaaggc ctgctggtcc tggactccct gggacaggta
     6301 gccggtgcgc aggtcggcga cgaggccgga gagcaaccac accgccacca ggaggtagac
     6361 cgccggggcg gcgaggtccg agcgccgcca cggcaggggg gacggctccg tcgtcgaccg
     6421 gtcccgactg gtgttcccag ctcggccggg ctgggtctgc gacgcgtcgt cgggggcgag
     6481 tgccgcggcc gtcacgcggg gcccgaggag tcccggggcc tcgtccgggt gctcgaacat
     6541 cgggtcgtgg gcctcgcccc gcaggtgcgc gatcgctcgg tcgtgcgcgg ccacccgacc
     6601 ctcgaggacc cggatgacct cgtcgacctg ctcgcgccgg taccccctca gctgggtgcg
     6661 cagcgcgtcg tcgagcggag cggtcatgcc gcggcccggg cgaggacccc gtcgacgacc
     6721 ctggcgaaca gcacggcggc cagccggtcg ccgatgggcc tcgtgagcgt cttcagcccg
     6781 ggcagccaca gttcctctgc ccacacgatc gacgagccgc cgggacgctc cgggtccgga
     6841 cgcaccgtga tgtcggccca gccgtgcagg aaccggccgg tcttgaccag tcgcaaccgg
     6901 tgcccgggct ccagggccgt cacgagcatg cggtccgcga tcgccagtgg ccccaggcgg
     6961 gtccaggcga tgacctcggc gccgagggcc aacccctgct cgggcacctc gacgtcggtc
     7021 aggggaacgt gcgcggtgtg ctcggccagg tcggtgaggg cggcaaaggc gacagcaggt
     7081 gccagcggcg tgcggcgggt gatcgtgcag gccatcggct cactgacctc ccgaccggtt
     7141 ctcgtcgagc agtcgcgcga gctcggcctc gcgttgcgcc agcgtctcgt gcaggacctc
     7201 caggcgggtg tcgacgtcgt cgatgcggta gccgcgcacc tcggtgtcga agcgcacggc
     7261 agccacgtcg ctcgcgctcg ggacctcggg gagccccgtc tccggggtcg tcgagacagc
     7321 agcgaccaac gggtcggcag gcacccgccc cgtggccacg ccgagcacga ccaggacgag
     7381 cgcgacggcg acgagcccga ggacgatgag agcaaccacg ccccgatcct acggcggcca
     7441 caccgcctct actctgacgt gcccgcgggt ggcgacggcg tggacgagcg tcgtgccgag
     7501 gcgtcggcgg cgatgatggt gcccaccgcc tcgtcgacgt cgtcggtgag ggtcagcagg
     7561 tcgaggtcac cggtgctgat cgtgccgcgc gcggctgcgg tgtcgcgcag ccagtcgagc
     7621 aggccacccc agtagtcacg accgagcagg acgatcggga agctggtcac cttctgcgtc
     7681 tgcacgaggg tcaccgcctc gaacagctcg tcgagcgtgc cgaacccgcc ggggagcacg
     7741 atgaagccct gcgcgtactt cacgaacatc gtcttgcggg cgaagaagta gcggaagttc
     7801 ac
//
LOCUS       DAOSOC010000116         7761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9385_length_7761_cov_8.765507, whole genome shotgun sequence.
ACCESSION   DAOSOC010000116 DAOSOC010000000
VERSION     DAOSOC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9385_length_7761_cov_8.765507"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PL014_09175"
     CDS             <1..976
                     /locus_tag="PL014_09175"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOT57303.1"
                     /translation="AVYFAGPGGSDGFLIENLTGNLDASPEALRWMFVGFFIAFAVKA
                     PMWPVHTWLPDAATEARPATAVLLVGVLDKVGTYGMIRFCLQLFPEASQWATPVVITL
                     AVISVIYGALLAIGQTDMMRLIAFTSISHFGFIVLGIFALTSVGGAGSTLYMVNHGFS
                     TAGLFLIAGFLVARRGSKRIPDYGGWQRVTPVLAGAFLVVGLSSLALPGLSSFVSEFL
                     VLQGTFMVYPWAAVIAALGIVLAALYILLMYQRVMTGPKPTFAEGEAPPDLSWREKVV
                     VAPIIASFLFLGFFPKPVLDLVNPAVDRTLQIVGVSDPAPANASGSEK"
     gene            973..2532
                     /gene="nuoN"
                     /locus_tag="PL014_09180"
     CDS             973..2532
                     /gene="nuoN"
                     /locus_tag="PL014_09180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HOT57304.1"
                     /translation="MNPEFSAVDIDYAAVAPMLIVVAGALIGVVVEAFAPRRSRHGIQ
                     VGLAVLTLVAAFVALVVWSRNNLTVTLGGSVVVDGVSVFLIGALLLMAALSVLIMAER
                     FGGVGSDAFTPMGASTPGSSQEDVATRAGYATSEVFPLTLFAVLGMMVFLTANDLITM
                     FVGLEVLSLPLYVLTGLARRRRLLSQEAALKYFLLGAFSSAFFLFGAALLYGFAGSSD
                     LRVIAAAISTTSSDFDGLLVPGVVLVLIGLLFKVGAVPFHSWTPDVYQGAPTPITGFM
                     AACTKLAAFGVILRVTYVGVSASRWDWSDGLWAIAAITMVVGAVLSVTQTDVKRLLAY
                     SSIAHAGFILVGVLAFSQAAIGGVMFYLVAYGFATVAAFGIIAMVRQGGSEASHLSQW
                     AGLGRRHPVVAGAFSFLLLAFAGIPLTSGFTAKFAAFAPAVEAGTGGVVLVVIAVLAS
                     AVTAFVYVRLIVLMYFTEASTEEGDEVVALTPSIPSTIGITVGVLVTAVLGIVPGFLL
                     DIASSASEFLL"
     gene            2538..3557
                     /locus_tag="PL014_09185"
     CDS             2538..3557
                     /locus_tag="PL014_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT57305.1"
                     /translation="MAATPAQRGTPSVLDLPGATADLRERLGAGLAAVNALIEERVDH
                     DDPFIAEAGAHLTAAGGKRFRPLLTLLTAELTHGINEQVVAAAAGVELTHLASLYHDD
                     VMDEADVRRGAPSANAAYGNSTAILVGDLLFGTASDIVADLGPDAVKIQAQTFIRLCS
                     GQIRDDRPCPAGTDPVEYYLGVLADKTGVLIATAARYGAMFSGGDEQTIETMREFGER
                     LGVAFQLADDLIDIESDADETGKTPGTDLREGKRTLPVLHVLASTDPADARLQALLTG
                     DLSDDDRLAEALTLLRAHPAMDLAREHTYAVARRAQDVLTALPDSDAKDALNALATGV
                     VTRVG"
     gene            complement(3718..5010)
                     /locus_tag="PL014_09190"
     CDS             complement(3718..5010)
                     /locus_tag="PL014_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT57306.1"
                     /translation="MTEDVETYVGDPPRTRWHRAWAVAGVTLAALVAAAAFRSSTGVL
                     LEPIEREFGWSRATTSGAVSLNLVLYGISAPFAAAAMERWGVRRTVTTALVVVGVASG
                     LTTVMTAPWQLWVLWGVFIGVGTGAMALVFGAIVANRWFHTQRGLVTGVFSAASATGQ
                     LIFLPVIARTATDAGWRAAALIVSALALLTGVLVVAFLRDRPSDVGVLPYGLPPGAVV
                     DAPQAPTASPAVVAVRVLRFASRTWVFWALALTFWICGWSTNGIIQTHFIPAAHDHGM
                     PSTTAAGLLALVGVFDIAGTIASGWLTDRVDPRWLLAGYYFGRALSLVALDAVLAPGI
                     VPGMWVFIVFYGLDWVATVPPTVALCRTHFGLERSGVVFGWVFASHMIGAGVGASVAG
                     LVRTDQGDYHWAWLIAAAMCFAAVALSLSIPRHPRIDQ"
     gene            5168..6313
                     /locus_tag="PL014_09195"
     CDS             5168..6313
                     /locus_tag="PL014_09195"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT57307.1"
                     /translation="MNPWESATAGLPSPLVAVDLDAFDANADDLVRRAGGLPIRVASK
                     SLRCRALLDRVLARPGFAGVMAYAVPEAVWLVTAGVRDVFVAYPSVDVEALRAVAADD
                     RLRSEVTITIDSVEHVRWLRDTLGAAARGLGVALDVDASLRVGPVHLGVRRSPTRTPA
                     QALAVVRAALGVGLDVRGVMTYDAQVAGLPDAGPHLRALKRRSVPEIADRRRAVVEAL
                     RAETDVRFVNAGGTGSLHRFAGDPVVTELAAGSGLYAPTLFDAYDDFSPRPAMAFALP
                     VVRRPAPGFATAFGGGYVASGEPGWARVPSPVRRGVKLVRTEAAGEVQTPLHGEGVGD
                     LAIGDRVWLRGAKAGELLERFDTVHLVSAGQLVDTVPSYRGEGRNFG"
     gene            6310..7611
                     /locus_tag="PL014_09200"
     CDS             6310..7611
                     /locus_tag="PL014_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HOT57308.1"
                     /translation="MTSWRNWGGNVVASPVQVRHATSADGVADAVRAAARAGLGVRVA
                     GSGHSFTPLVATDGMLLHVDGLSGVIGVDRERNRVRVLAGTPLHVLNPALQRLGLALP
                     NLGDVDRQTISGAIATGTHGTGTRRQGIAAAVTGLTLVLADGSTLECSRDVQPDVFAA
                     AAVGLGALGVVTEVELACVPAYRLHAKESSEALSDLLPRLQAEADTHDHLDLHWFPHT
                     DRVLVKRNDVVGAQQGPPPLAPWRARLDDDLLANRVFEVINRGAAALPASVPVINRAT
                     ARVLGAREFADDSWKVFCSSREVRFVESEYGVPRHALEAVLAELRAWFDRTRAPVPFP
                     VEVRFIGADDVWLSTAHERDNAYVAVHQYHRMDPRPLFEAFEAIVAEHAGRPHWGKVH
                     TLGADRLRELYPRLDDFRAVRERLDPGRRFTNAHVRHLLGD"
BASE COUNT         1018 a         2834 c         2706 g         1203 t
ORIGIN      
        1 cgccgtgtac ttcgcgggac cgggtgggtc agacggcttc ctcatcgaga acctcacggg
       61 caacctcgat gcctcgccgg aggccctgcg ctggatgttc gtcggcttct tcatcgcctt
      121 cgccgtcaag gccccgatgt ggccggtcca cacgtggctg cccgacgccg ccaccgaggc
      181 acgccccgcg accgcggtgc tgctcgtcgg cgtgctcgac aaggtcggca cctacggcat
      241 gatccgcttc tgcctccagc tgttcccgga ggcctcgcag tgggcgaccc cggtcgtcat
      301 caccctcgcg gtcatctcgg tgatctacgg tgcgctgctc gcgatcggcc agaccgacat
      361 gatgcggctc atcgcgttca cctcgatcag ccacttcggc ttcatcgtcc tgggcatctt
      421 cgccctgacg agtgtcggcg gcgccggctc gacgctgtac atggtcaacc acggcttctc
      481 cacggccggg ttgttcctga tcgccgggtt cctcgtggcc cgccgcggca gcaagcggat
      541 ccccgactac ggcggctggc agcgggtcac ccccgtgctc gccggagcct tcctcgtcgt
      601 cggcctgtcc agcctggccc tgcccggcct gtcgtcgttc gtctccgagt tcctcgtcct
      661 gcagggcacg ttcatggtct acccgtgggc agccgtcatc gccgccctgg gcatcgtcct
      721 cgcggccctg tacatcctgc tcatgtacca gcgcgtcatg accggcccga agccgacctt
      781 cgccgagggc gaggccccac cggacctgtc ctggcgggag aaggtcgtcg tcgcgccgat
      841 catcgcctcg ttcctcttcc tcggcttctt cccgaagccg gtgctggacc tggtcaaccc
      901 ggccgtggac cggacgctgc agatcgtcgg cgtctcggat ccggcccccg ccaatgcctc
      961 cgggagtgag aagtgaaccc cgagttctcg gccgtcgaca tcgactacgc cgccgtcgcc
     1021 ccgatgctca tcgtcgtcgc gggtgccctc atcggtgtcg tcgtggaggc cttcgctccg
     1081 cgacgcagcc gccacggcat ccaggtcggc ctcgccgtcc tcaccctcgt cgcggccttc
     1141 gtggctctcg tggtgtggtc acgcaacaac ctcacggtga ccctcggtgg gtccgtcgtg
     1201 gtcgacgggg tgtcggtctt cctcatcggt gccctgctgc tgatggccgc gctcagcgtg
     1261 ctcatcatgg ccgagcgctt cggcggagtc ggctcggacg cgttcacccc catgggtgcc
     1321 tcgacgcccg gctcgtccca ggaggacgtc gccacacggg cggggtacgc cacctccgag
     1381 gtcttcccgc tcacgctgtt cgcggtgctc gggatgatgg tcttcctcac cgccaacgac
     1441 ctcatcacga tgttcgtcgg actcgaggtg ctctcgctgc cgctctacgt cctcaccgga
     1501 ctggcgcgcc gccgccgtct gctctcgcag gaggccgcgc tcaagtactt cctgctcggc
     1561 gccttctcga gcgcgttctt cctcttcggt gcggcgctgc tctacggctt cgccgggtcc
     1621 tccgacctgc gggtcatcgc cgccgcgatc tccacgacgt ccagtgactt cgacggtctc
     1681 ctggtgcccg gggtcgtgct cgtgctcatc ggcctgttgt tcaaggtcgg cgccgtgccg
     1741 ttccactcgt ggacccccga cgtctaccag ggtgcgccga ccccgatcac cggcttcatg
     1801 gcggcctgca ccaagctcgc cgcgttcggg gtgatcctgc gggtgacgta cgtgggtgtc
     1861 agcgcgagcc gttgggactg gtcggacggg ctctgggcga tcgccgcgat cacgatggtc
     1921 gtcggcgcgg tgctctcggt cacccagacc gacgtcaagc gactgctcgc ctactcctcg
     1981 atcgcccacg cggggttcat cctcgtcggt gtcctggcgt tctcgcaggc ggccatcggc
     2041 ggggtgatgt tctacctcgt cgcctacggt ttcgcgacgg tggcggcgtt cggcatcatc
     2101 gccatggtcc gtcagggtgg gtccgaggcc tcgcacctct cgcagtgggc cggcctgggc
     2161 cggcgtcacc cggtcgtggc cggggcgttc tcgttcctgc tgctcgcgtt cgccggtatc
     2221 ccgctgacct cgggcttcac cgccaagttc gcggcgttcg cgccggccgt ggaggcgggc
     2281 accggaggtg tcgtcctcgt cgtcatcgcc gtgctggcct cggcggtcac ggccttcgtc
     2341 tacgtccggt tgatcgtcct gatgtacttc accgaggcct ccaccgagga gggcgacgag
     2401 gtcgtcgcgc tcacgccgtc gatcccgtcg acgatcggca tcaccgtcgg tgtcctcgtg
     2461 acggccgtgc tcggcatcgt gcccggcttc ctcctcgaca tcgcgagctc ggcctccgag
     2521 ttcctcctgt gacgcccgtg gcggccaccc ctgcccagcg cggcaccccc tcggtgctcg
     2581 acctgccggg ggcgactgcc gacctgcgtg agcgcctcgg ggcggggctc gccgcggtca
     2641 acgcgctcat cgaggagcgc gtcgaccacg acgacccgtt catcgccgag gccggagcac
     2701 acctcacggc ggccgggggc aaacgcttcc gtcccctgct gaccctgctc acggccgagc
     2761 tcacccacgg catcaacgag caggtcgtcg cggcagccgc cggcgtcgaa ctgacgcacc
     2821 tcgcgtcgtt gtaccacgac gacgtcatgg acgaggccga cgtgcgtcgg ggggcgccga
     2881 gcgccaacgc ggcctatggc aactccacgg cgatcctcgt cggtgacctg ctgttcggaa
     2941 cggcctccga catcgtcgcc gacctcggcc cggatgccgt gaagatccag gcgcagacgt
     3001 tcatccggtt gtgctcgggg cagatccgcg acgaccggcc gtgccccgcg ggcaccgacc
     3061 cggtggagta ctacctcggg gtgctggccg acaagacggg tgtgctgatc gccaccgctg
     3121 cgcggtacgg ggcgatgttc tccggtggcg acgagcagac catcgagacg atgcgtgagt
     3181 tcggcgaacg cctcggggtg gcgttccagc tggcggacga cctcatcgac atcgagtccg
     3241 acgccgacga gacgggcaag acccccggca ccgacctccg tgagggcaag cgcaccctgc
     3301 cggtgctgca cgtcctcgcc tcgaccgatc ctgccgatgc ccgactccag gcgctgttga
     3361 ccggggacct ctcggatgac gaccggcttg ccgaggcgct gaccctgctg cgagcccacc
     3421 cggcgatgga cctggctcgt gagcacacgt atgccgtggc ccgtcgcgcg caggacgtgt
     3481 tgaccgccct cccggacagc gacgccaagg acgccctgaa cgccctggcc accggggtcg
     3541 tcacgcgcgt cggctgaccc tggcgggcag gggggcggcg gccctcaccc gacttcgtgc
     3601 gacttgatga ccgttgccca ggccaaaaca ggcccgaaag cgtaggtaag tcgcacgaag
     3661 tcggagccaa cggggccagt cggtacgggt gtctgtggct tgcgtacctg gagtgaccta
     3721 ttggtcgatg cggggatgcc gggggatcga cagggacagg gccaccgcgg cgaagcacat
     3781 cgccgccgcg atcagccacg cccagtggta gtcgccctgg tcggtgcgca ccagccctgc
     3841 gacgctggct cccaccccgg ccccgatcat gtgggacgcg aacacccacc cgaacacgac
     3901 ccccgagcgt tcgagaccga agtgggttcg gcacagggcc acggtcggtg gcacggtggc
     3961 cacccagtcc aggccgtaga agacgatgaa cacccacatg ccggggacga tgccgggggc
     4021 cagcacggca tccagcgcca ccagggacag cgcgcgcccg aagtagtagc cggcgagcag
     4081 ccaccgaggg tcgacccggt cggtgagcca ccccgacgcg atcgtgccgg cgatgtcgaa
     4141 caccccgacg agggcgagca acccggctgc cgtggtggac ggcatcccgt ggtcatgggc
     4201 cgcggggatg aagtgggtct ggatgatgcc gttggtcgac cagccacaga tccagaacgt
     4261 cagggccagt gcccagaaca cccaggtgcg gctggcgaac cgcagcaccc gaacggcgac
     4321 gaccgcgggt gaggcggtcg gtgcctgtgg cgcgtcgacc actgcccccg gtggcagccc
     4381 gtagggcagc accccgacgt cgctgggtcg gtcgcgcagg aaggccacga cgagcacccc
     4441 ggtgaggagg gcgagcgcgc tgacgatgag ggcggcggcc cgccacccgg catccgtcgc
     4501 ggtgcgcgcg atgacaggca ggaagatcag ctggccggtc gcactcgccg ccgagaacac
     4561 gccggtgacc agtccgcgct gcgtgtggaa ccagcggttc gcgacgatcg caccgaacac
     4621 gagcgccatg gctccggtgc cgaccccgat gaacacgccc cagagcaccc agagctgcca
     4681 cggcgcggtc atcaccgtcg tgagcccgct cgccaccccg acgacgacca gcgccgtggt
     4741 caccgttcgc cgcacccccc accgctccat cgcggcggcg gcgaagggtg cactgatgcc
     4801 gtagagcacg aggttgaggc tgaccgcgcc ggacgtcgtg gcccgcgacc agccgaactc
     4861 ccgctcgatg ggttcgagga gcaccccggt cgaggaccgg aacgcggcgg ctgcgacgag
     4921 ggccgcgagc gtcacgccag ccacagccca tgcgcggtgc cagcgcgtgc ggggcgggtc
     4981 ccccacgtag gtctcgacgt cctcggtcac cgacgcagtc tgccaccggg cgaacgaggc
     5041 cggcggggtg tgtccggggc gggatgtcgg gacgatgtcg ggtgcgggat gtccagcggg
     5101 gagtgtccgg cgcggtggtg ccccaacagc cgacgggccc gaggtcgggg ttcagtaggg
     5161 tcggggcgtg aacccgtggg aatcggcgac ggcagggctg ccgtcaccgc tcgtcgccgt
     5221 cgacctcgac gcgttcgacg ccaacgccga cgacctcgtg cgtcgtgccg gcggcctgcc
     5281 gatcagggtc gcctcgaagt cactgcggtg ccgtgccctg ctcgaccggg tgctcgcccg
     5341 cccggggttc gccggcgtca tggcgtatgc cgtgcccgag gcggtctggc tcgtcaccgc
     5401 cggggtccgc gacgtgttcg tcgcctaccc gagcgtcgac gtcgaagcct tgcgggcggt
     5461 cgctgctgac gaccggctgc gttccgaggt gacgatcacc atcgactccg tcgagcacgt
     5521 ccgctggctg cgcgacaccc tgggtgccgc cgcccgtggc ctcggcgtgg cgctcgacgt
     5581 cgacgcctcg cttcgtgtcg gcccggtgca cctcggcgtg cggcgctcgc cgacccgtac
     5641 gcccgcgcag gccctcgccg tggtgcgagc agccctcggg gtggggctcg acgtgcgggg
     5701 cgtcatgacc tacgacgcgc aggtcgccgg gctgccggat gccgggccgc accttcgcgc
     5761 cctgaagcgc cgctcggtcc cggagatcgc cgaccggcgc cgcgccgtcg tcgaggcact
     5821 gcgggccgag accgacgtgc ggttcgtcaa cgccggtggg acgggcagcc tgcaccgctt
     5881 cgcgggcgac ccggtcgtca ccgagctggc cgccgggtcc ggcctgtacg cgcccacgct
     5941 cttcgacgcc tacgacgact tctccccgcg accggcgatg gccttcgccc tgcccgtggt
     6001 gcggcgtccg gcgccgggct tcgccacggc cttcggtggg gggtacgtcg ccagcggaga
     6061 gccgggctgg gcgcgggtcc cctcaccggt gcgccgcggc gtgaagctgg tgcgcaccga
     6121 ggctgccggc gaggtgcaga cgccgctgca cggggagggc gtcggcgacc tggccatcgg
     6181 tgaccgcgtc tggcttcgtg gggccaaggc cggcgagctc ctcgaacggt tcgacaccgt
     6241 gcacctcgtg agcgccggtc agctggtcga caccgtgccg tcctaccgcg gcgaaggaag
     6301 gaacttcgga tgaccagctg gcgcaactgg ggcggcaacg tcgtggcgtc cccagtgcag
     6361 gttcggcacg ccaccagcgc cgacggggtc gcggatgccg tacgcgccgc cgcgcgtgcc
     6421 gggctcggtg tgcgcgtggc cgggtccggg cactccttca ccccgctggt cgcgaccgac
     6481 gggatgctgc tgcacgtcga cggcctgagc ggggtgatcg gcgtcgaccg ggagcgcaac
     6541 cgggtcaggg tgctggccgg aacaccgttg cacgtgctca acccggcgct ccagcggctc
     6601 gggctggccc tgccgaacct cggcgacgtc gatcgccaga ccatctccgg ggccatcgcc
     6661 accggaaccc acggcacggg cacccgccga caggggatcg cggccgccgt gacggggctg
     6721 accctcgtgc tggcggacgg gagcacgctg gagtgctcgc gcgacgtcca gccggatgtc
     6781 ttcgcggcgg cggccgtcgg gctgggcgcc ctcggcgtcg tcaccgaggt cgagctcgcg
     6841 tgtgtacccg cgtaccggct gcacgccaag gagtcgagcg aggcgctcag cgacctgttg
     6901 ccccggctcc aggccgaggc agacacccac gaccacctcg acctgcactg gttcccccac
     6961 accgaccgcg tgctggtcaa gcgcaacgac gtcgtcggcg cgcagcaggg cccaccgccg
     7021 ctcgccccgt ggcgggcacg gctcgacgac gacctcctcg cgaaccgggt cttcgaggtg
     7081 atcaaccgcg gggccgccgc gctcccggca tccgtgccgg tgatcaaccg ggcgacggcg
     7141 cgcgtgctcg gggcgcgcga gttcgccgac gactcctgga aggtgttctg ttcctcgcgg
     7201 gaggtgcgct tcgtcgagag cgagtacggt gtgccgcgcc acgcgctcga ggcggtgctg
     7261 gcggaactgc gggcgtggtt cgaccgcacc cgcgcccccg ttccgttccc ggtggaggtg
     7321 cggttcatcg gggccgacga cgtgtggttg tcgacggcgc atgaacggga caacgcctac
     7381 gtggccgtgc accagtacca ccggatggac ccgcggccgc tgttcgaggc cttcgaggcg
     7441 atcgtggccg agcacgccgg gcggccgcac tggggcaagg ttcacaccct cggtgccgac
     7501 cggctgcgcg agctgtaccc gcggctcgac gacttccgag cggtccgcga gcgcctcgac
     7561 cccggtcggc gcttcaccaa cgcccacgtc cgccacctcc tcggggactg agccaccatg
     7621 cgggtcaccg aacgtgagat cacggccgag gtcgagctct gccggcccga cggcaccctg
     7681 aacccggacg cggtcgggtg gacccggcat ccgctccacg acacgagccg gatcggccga
     7741 ggccgtcgag ggtggggccg c
//
LOCUS       DAOSOC010000117         7716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9447_length_7716_cov_10.250098, whole genome shotgun sequence.
ACCESSION   DAOSOC010000117 DAOSOC010000000
VERSION     DAOSOC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9447_length_7716_cov_10.250098"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /gene="rpsN"
                     /locus_tag="PL014_09205"
     CDS             <1..107
                     /gene="rpsN"
                     /locus_tag="PL014_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="located in the peptidyl transferase center and
                     involved in assembly of 30S ribosome subunit; similar to
                     what is observed with proteins L31 and L33, some proteins
                     in this family contain CXXC motifs that are involved in
                     zinc binding; if two copies are present in a genome, then
                     the duplicated copy appears to have lost the zinc-binding
                     motif and is instead regulated by zinc; the proteins in
                     this group appear to contain the zinc-binding motif;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOT57309.1"
                     /translation="GRPHSVYRKFGLCRICLREMAHRGELPGVTKSSW"
     gene            250..648
                     /gene="rpsH"
                     /locus_tag="PL014_09210"
     CDS             250..648
                     /gene="rpsH"
                     /locus_tag="PL014_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOT57310.1"
                     /translation="MTMTDPIADMLTRVRNANSAHKDAVSMPFSKLKSHIAEILEAEG
                     YIAGWTVEDAEVGQTLTINLKYGPNRERSIAGVRRVSKPGLRVYAKSTNLPKVLGGLG
                     VAIISTSSGLLTDRQAANKGVGGEVLAYIW"
     gene            661..1197
                     /gene="rplF"
                     /locus_tag="PL014_09215"
     CDS             661..1197
                     /gene="rplF"
                     /locus_tag="PL014_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOT57311.1"
                     /translation="MSRIGRLPVTVPSGVDIAIDGQTVKVKGPKGELSHVVAEPITVE
                     TNDGVLEVKRPDDARTSRSLHGLTRSLINNMVLGVTDGYEKKLEIHGTGYRVLAKGSN
                     LEFALGYSHSITVEPPAGITFVVENQTRFSVQGIDKQLVGEVAANIRKLRKPDPYKGK
                     GVRYAGEQIRRKVGKAGK"
     gene            1201..1575
                     /gene="rplR"
                     /locus_tag="PL014_09220"
     CDS             1201..1575
                     /gene="rplR"
                     /locus_tag="PL014_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOT57312.1"
                     /translation="MALIVKRAKGKSASRARRHFRVRKVVTGTTGRPRLVVSRSSRHV
                     FVQVVDDTVGKTLASASTMEADLRSFDGDKTAKAKKVGELVAERAKAAGVEAVVFDRG
                     GNKYHGRVAAIADGAREGGLSL"
     gene            1619..2269
                     /gene="rpsE"
                     /locus_tag="PL014_09225"
     CDS             1619..2269
                     /gene="rpsE"
                     /locus_tag="PL014_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOT57313.1"
                     /translation="MPGPQRRGTGGPATAERGGREGRGGREGGRGGRDAREPEKSQYI
                     ERVVTINRVAKVVKGGRRFSFTALVVVGDGDGTVGVGYGKAKEVPAAIAKGVEEAKKQ
                     FFKVPRIQGTIPHPVQGEAAAGVVMLRPASPGTGVIAGGPVRAVLECAGIHDVLSKSL
                     GSDNAINIVHAAVAALKGLERPEQVAARRGLPVDRVAPAAMLRAQAAGIAEAKAGA"
     gene            2269..2451
                     /gene="rpmD"
                     /locus_tag="PL014_09230"
     CDS             2269..2451
                     /gene="rpmD"
                     /locus_tag="PL014_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOT57314.1"
                     /translation="MARLKVTQTRSEIGGKQNQRDTLRSLGLKRIGDVVVKEDRPEIR
                     GMVKTVTHLVAVEEVE"
     gene            2454..2939
                     /gene="rplO"
                     /locus_tag="PL014_09235"
     CDS             2454..2939
                     /gene="rplO"
                     /locus_tag="PL014_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOT57315.1"
                     /translation="MADTTKAAQAAEGAAALKVHHLRPAPGAKSAKIRVGRGEGGRRG
                     KTAGRGTKGTKARYQVPEGFQGGTTPLHMRFPKLRGFKNPFRTEYQVVNLDKLSSLFP
                     EGGDVSIEDLVSKGAVRNGQLVKVLGSGEITVAVNVTADAFSASAKQKIEAAGGSATT
                     A"
     gene            3111..4424
                     /gene="secY"
                     /locus_tag="PL014_09240"
     CDS             3111..4424
                     /gene="secY"
                     /locus_tag="PL014_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOT57316.1"
                     /translation="MLTAFGRAFKTPDLRRKILFTLSIITLYRLGAHVPTPGVSYPLV
                     QQCISGADNSNGLLGLVNLFSGGALLQLSIFALGIMPYITASIIVQLLTVVIPRFEAL
                     KKEGQAGQTKMTQYTRYLTIALAVLQSSTLITFAQNPASLFNNPACTNILSNDSVVTI
                     LIMVLTMTAGTGVVMWMGELITDKGIGNGMSLLIFTSIAAGFPASLWAIQQRSGWDVF
                     IGIILMGFVVISLVVFVEQSQRRIPVQYAKRMVGRRVYGGTSTYIPLKVNMAGVIPVI
                     FASSLMSLPMLLAQFNQRADGNNAGWVDWINANLVTGDKPMYMALYIGLILFFTFFYV
                     SITFNPTEVADNMKKYGGFIPGIRAGRPTAEYLSYVLTRITVPGAIYLALISLIPLIA
                     LVLVDANQNFPFGGTSILIIVGVGLETVKQIEAQLQQRHYEGFLR"
     gene            4424..4984
                     /locus_tag="PL014_09245"
     CDS             4424..4984
                     /locus_tag="PL014_09245"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOT57317.1"
                     /translation="MRLLILGPPGAGKGTQAARIAEHFGIPAISTGDIFRANIKNETE
                     LGLQVKSILASGGFVSDEVTNAIVADRLAQDDCQAGFLLDGYPRTLPQVAALDELLAS
                     KGQTLEHVLELTVDEDAVVQRLLKRAAVEGRADDTEDVIRERMAIYHRETKPLSDTFR
                     ERGVLAEVDGMGEVDEVTERVLATLA"
     gene            5114..5968
                     /gene="map"
                     /locus_tag="PL014_09250"
     CDS             5114..5968
                     /gene="map"
                     /locus_tag="PL014_09250"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT57318.1"
                     /translation="MGLFNRDRCEGKNPEQLAGMRTAGLLVGRTLEVVAAAVHPGMTT
                     LDLDTLAETHIRDHGGIPNFQLVPGYRHTLCTSVNDEIVHGMPGSRVLREGDVLSIDC
                     GAQVGLWNGDAAISVVVGGREAGSAQDLALIDDTETSLWAGIAALRVGQSLYAVGAAV
                     EDSLTASGEERGWEYGIVEDYVGHGIGTSMHMDPNVPNYRVSGKGPKVVEGTTICIEP
                     MVTLGEQDNHVLDDQWTVATNDRSRAAHWEHSIAATSAGLCVLTAIDGGAQRLAAMGA
                     PFAAIDPA"
     gene            6155..>7716
                     /locus_tag="PL014_09255"
     CDS             6155..>7716
                     /locus_tag="PL014_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HOT57319.1"
                     /translation="MSDPSAVPSTIDLAAGFPEQTREDWQVLVAAVLNKGRAPDAQLD
                     GAAAEQALRRHLEGGLEVDAVYLREDRTLGSPGRMPFTRGRAVRDATAPWDVRQRHDD
                     PDVAVTRAAVLDDLEHGVTSVWLHIGDDGLAVGDLREALADVRLDLAPVVVSSHTHQV
                     EAAQALLDHVGDHPSVGGNLGLDPLAAAARLGAEPDLTPLADLVRATATRPGWRAITV
                     DATVLHDAGATDVDALAAAVATGVAYVRHLEAAGIAPAQAFDQVEFRVGATADQFLTA
                     AALRALRRVWARVGEASGVPEASRGARTHAVTSLRMFTREDPWVNVLRSTLATFGASI
                     GGADAITVLPFDTVLGLPERLGRRLARNTQILLADEANVARVTDPGGGSWYLESLTDD
                     VASAVWARFQEVEASGGAVQALADGLFASWVADAVAQRDRDLATRSRPLTGVSMFPLV
                     GEVTPDRRARADLPTAAGGFAPRRDATAYKALRDRARELGQPAVVVHTLGTRRDFGAR
                     QAFVTNLLAAGG"
BASE COUNT         1223 a         2794 c         2519 g         1180 t
ORIGIN      
        1 gcggccgtcc gcactcggtc taccgcaagt tcggcctgtg ccggatctgc ctgcgggaga
       61 tggcccaccg cggcgagctc cccggcgtca ccaagtcttc ctggtgaacc cacgggatgc
      121 cggccggtca ccgacccggc atcccacccg ccgcaccacg cggcatccat gaaccacacc
      181 atctacatcg caggtccgcc cggccacccg gccgggagga aaccgtgatg agggagggcg
      241 gaagccccaa tgaccatgac cgacccgatc gcggacatgt tgacccgcgt ccgtaacgcc
      301 aactcggcgc acaaggacgc agtctccatg ccgttctcga agctcaagag ccacatcgcg
      361 gagatcctcg aggccgaggg ttacatcgcc ggttggaccg tcgaggacgc cgaggtgggc
      421 cagacgctca ccatcaacct caagtacggc cccaaccgcg agcgttccat cgccggtgtg
      481 cgccgcgtct ccaagccggg cctgcgcgtg tacgccaagt ccaccaacct gccgaaggtt
      541 ctcggtggcc tgggtgtcgc gatcatctcg acgtcgtccg gcctgctgac tgaccgtcag
      601 gccgccaaca agggtgtggg tggggaagtc ctcgcctaca tctggtaagg ggttactgac
      661 atgtcacgca tcggacgtct ccccgtcacc gtcccctcgg gtgtcgacat cgccatcgat
      721 ggccagaccg tcaaggtcaa gggccccaag ggcgagctca gccacgtcgt tgccgagccg
      781 atcaccgtcg agaccaatga cggtgttctc gaggtcaagc gccccgacga cgcgcgcacg
      841 tcgcgctcgc tgcacggcct gacccgctcg ctcatcaaca acatggtgct cggcgtcacc
      901 gacggctacg agaagaagtt ggagatccac ggcaccggtt accgcgtgct ggccaagggc
      961 tccaacctcg agttcgccct cggctactcg cactcgatca ccgtcgagcc gcccgccggc
     1021 atcaccttcg tggtcgagaa ccagacccgg ttctcggtcc agggcatcga caagcagctc
     1081 gtcggcgagg tcgccgcgaa catccgcaag ctccgcaagc ccgacccgta caagggcaag
     1141 ggcgtccgct acgcgggcga gcagatccgc cgcaaggtcg gaaaggctgg taagtaagcc
     1201 atggcactga tcgtcaagcg cgccaagggc aagagcgcct cccgcgcccg tcgccacttc
     1261 cgcgtccgca aggtcgtcac cggtaccacc gggcgtcccc gcctcgtcgt gtcccgctcg
     1321 tcgcgccacg tcttcgtcca ggtcgtcgac gacaccgtcg gcaagacctt ggcgtcggcc
     1381 tccaccatgg aggcggacct gcggtccttc gatggtgaca agaccgccaa ggccaagaag
     1441 gtcggcgagc tcgtcgccga gcgcgccaag gctgccggcg tcgaggccgt cgtcttcgac
     1501 cgtggcggca acaagtacca cggtcgcgtc gccgccatcg ctgatggcgc tcgcgaaggc
     1561 ggtctgtccc tgtgatcgcc acccagaaca cccccgttgt tgagatgagg aacatctgat
     1621 gccaggaccc cagcgtcgag gcaccggcgg acccgcaacc gccgagcgcg gcggccgcga
     1681 aggtcgcggt ggccgtgagg gcggtcgcgg cggtcgtgac gcccgcgagc ccgagaagag
     1741 ccagtacatc gagcgcgtcg tgacgatcaa ccgcgtcgcc aaggtcgtca agggtggtcg
     1801 tcgcttcagc ttcaccgcgc tcgtcgtcgt cggtgacggt gacggcaccg tcggtgtcgg
     1861 ctacggcaag gccaaggagg tgcccgcggc gatcgccaag ggcgtcgagg aggcgaagaa
     1921 gcagttcttc aaggtccctc ggatccaggg caccatcccg caccccgtcc agggtgaggc
     1981 cgctgccggt gtcgtcatgc tccgtcccgc cagccccggt accggtgtca tcgccggtgg
     2041 tccggtgcgt gcggtcctgg agtgcgccgg catccacgac gtgctgtcca agagcctcgg
     2101 ctcggacaac gcgatcaaca tcgtccacgc ggcggtcgcg gccctgaagg gtctcgagcg
     2161 tccggagcag gtcgcggcac gccgtggcct cccggtggac cgagtcgccc ccgcggcgat
     2221 gctgcgtgct caggccgccg gcatcgccga ggcaaaggct ggtgcgtgat ggcccgtctc
     2281 aaggtgaccc agacccgttc cgagatcggt ggcaagcaga accagcgtga cacgctgcgc
     2341 agcctcggtc tgaagcgcat cggcgacgtc gtcgtcaagg aggaccgtcc cgagatccgc
     2401 gggatggtca agacagtgac ccacctggtc gctgtcgagg aggttgagtg aacatggcag
     2461 acaccaccaa ggccgcacag gccgccgagg gtgccgcagc gctgaaggtg caccaccttc
     2521 gtcccgcgcc gggcgccaag tccgccaaga tccgcgtcgg tcgcggtgaa ggtggccgtc
     2581 gtggcaagac cgccggtcgt ggtaccaagg gcaccaaggc ccggtaccag gtgccggagg
     2641 gcttccaggg tggcaccacg ccgctgcaca tgcggttccc gaagcttcgc ggcttcaaga
     2701 acccgttccg caccgagtac caggtcgtga acctcgacaa gctcagctcg ctgttccccg
     2761 agggtggcga cgtgagcatc gaggacctcg tgtccaaggg tgccgtgcgc aacggacagc
     2821 tcgtgaaggt gctcggcagc ggtgagatca ccgtggccgt gaacgtcacg gccgacgcgt
     2881 tctccgcgag cgccaagcag aagatcgagg cggcgggcgg ctcggcgacg accgcctgac
     2941 cctctgcatt gaccggatgc cgtagggctg ggtcccggga cacctcggga acctgctcta
     3001 cggcatccgg cgttcaagcc ccgatacgcg ccgtgccctg tgcggcgggt atcttgtgac
     3061 ggattgttga cgacgcggcc accacaccgg gctgcacctc tggaggacct gtgctcaccg
     3121 ctttcggccg cgcgttcaag acgccggacc tgcgcaggaa gattctgttc accctgtcga
     3181 tcatcacgct gtaccgcctc ggcgcgcacg tgccgacccc gggtgtcagc tacccgctcg
     3241 tgcagcagtg catcagcggc gcggacaaca gcaacgggct gctcggcctg gtcaacctgt
     3301 tcagcggcgg cgcgctgttg cagttgtcga tcttcgcgct cgggatcatg ccgtacatca
     3361 cggccagcat catcgtgcag ctcttgacgg tggtgatccc gcggttcgag gccctgaaga
     3421 aggagggcca ggcgggccag acgaagatga cgcagtacac gcgctatctg accatcgcgt
     3481 tggccgtgct gcagtcctcg acgctcatca ccttcgcgca gaacccggcc tcgctgttca
     3541 acaacccggc gtgcacgaac atcctcagca acgactcggt cgtgaccatc ctcatcatgg
     3601 tgctgaccat gacggccggt accggtgtcg tcatgtggat gggtgagctg atcaccgaca
     3661 agggcatcgg caacggcatg tccctgctga tcttcacgtc catcgccgcc ggcttcccgg
     3721 cctcgctgtg ggccatccag cagcgctccg gctgggacgt cttcatcggc atcatcctca
     3781 tgggcttcgt cgtcatctcg ctcgtcgtgt tcgtcgagca gagccagcgc cgcatccccg
     3841 tgcagtacgc caagcggatg gtggggcggc gcgtctacgg cggcacctcg acctacatcc
     3901 cgctcaaggt gaacatggcc ggtgtcatcc ccgtcatctt cgccagctcg ctgatgtccc
     3961 tgcccatgct gctggcgcag ttcaaccagc gcgccgacgg caacaacgcc ggctgggtcg
     4021 actggatcaa cgccaacctc gtgaccggtg acaagcccat gtacatggcg ctctacatcg
     4081 gcctcatcct cttcttcacg ttcttctacg tgtcgatcac gttcaacccg accgaagtcg
     4141 ccgacaacat gaagaagtac ggcggcttca tcccgggtat tcgcgccggg cgacccacgg
     4201 ccgagtacct cagctacgtc cttacccgga tcacggtgcc gggcgcgatc tacctcgcgc
     4261 tcatctcgct catcccgctg atcgcgctcg tcctcgtcga tgccaaccag aacttcccct
     4321 tcggtggtac ctccatcctc atcatcgtgg gtgtcggtct ggagacggtg aagcagatcg
     4381 aggcccagct ccagcagcgc cactatgaag ggttcctccg ctgatgcgtc ttctcatcct
     4441 cggccctccc ggtgccggca agggcaccca ggcggcccgg atcgccgagc acttcggcat
     4501 tccggcgatc tccaccggtg acatcttccg ggccaacatc aagaacgaga ccgagctcgg
     4561 gctccaggtc aagtcgatcc tggcgtcggg gggcttcgtc agtgacgagg tgaccaacgc
     4621 gatcgtcgcc gaccgcctgg cgcaggacga ctgccaggca ggcttccttc tcgacggtta
     4681 cccgcgcacg ctgccgcagg tggccgcgct cgatgagctg ctggcgtcca agggccagac
     4741 gctcgagcac gtgctcgagc tgaccgtcga cgaggatgcc gtggtgcagc ggcttctcaa
     4801 gcgcgccgcg gtcgagggcc gggccgacga caccgaggac gtcatccgcg agcggatggc
     4861 gatctaccac cgcgagacca agccgctgtc tgacaccttc cgtgagcgcg gcgtcctcgc
     4921 cgaggtcgac ggcatgggcg aggtcgacga ggtcacggag cgggtgctgg ccaccctggc
     4981 ctgatccggc accagccgat ccaacctcgg tcgctcagca acgcagaagt tcggagggca
     5041 cgcagcaatt gctgcttgcc atccgaactt ctgcgttgcg ggccctgccg aaaccgcggg
     5101 tagggtttcg cgcatgggtc ttttcaaccg tgaccgctgc gagggcaaga accccgagca
     5161 gctcgccggc atgcgcaccg ccggtctcct cgtcggccgc acgctcgagg tggtggccgc
     5221 cgcggtccat ccgggcatga cgacgctcga cctcgacacg ttggctgaga cccacatccg
     5281 cgaccacggc ggcatcccga acttccagct cgtccccggg taccggcaca ccctgtgcac
     5341 gagcgtgaac gacgagatcg tgcacggcat gccgggctcg cgcgtcctgc gtgaagggga
     5401 cgtgctctcc atcgactgcg gcgcccaggt gggcctgtgg aacggcgacg ccgccatcag
     5461 cgtggtcgtc ggcggtcgcg aggccggctc tgcccaggac ctcgccctca tcgacgacac
     5521 tgagacctcc ctgtgggcgg gcatcgccgc cctgcgcgtg gggcagagcc tgtatgccgt
     5581 cggcgccgcc gtcgaggaca gcctcaccgc ctcgggggag gagcgcggct gggagtacgg
     5641 catcgtcgag gactacgtgg gccacggcat cggcacgtcg atgcacatgg accccaacgt
     5701 gccgaactac cgggtgtcgg gcaagggacc caaggtcgtc gagggcacga cgatctgcat
     5761 cgagccgatg gtcaccctcg gtgagcagga caaccacgtg ctcgacgacc agtggaccgt
     5821 cgccaccaac gaccggtcgc gcgcagccca ctgggagcac tcgatcgccg cgacctcggc
     5881 cgggttgtgc gtgctcaccg cgatcgacgg tggagcgcag cgactggctg ccatgggcgc
     5941 accgttcgcc gcgatcgacc ccgcctgacc gtcggacgac cccgcctgac cgggccccgg
     6001 gcccacccac gcggccgttc gccgacgact cgaacccgcc gaccgcacac gctcgcggtg
     6061 tgcccgatct ctcgctgacc ccccgccgcg acgccgttag ggtggcctgc ggggcccgtc
     6121 tcgcggcccc gcccaccgtc gggaagggag cacgatgtcg gacccgtccg ccgtgccgag
     6181 caccatcgat ctcgctgcag gctttcccga gcagacccgc gaggactggc aggtgctggt
     6241 cgcggccgtg ctcaacaagg ggcgagcacc cgacgcacag ctcgacggtg ccgccgccga
     6301 gcaggcactg cgtcgccatc tcgagggtgg gctcgaggtg gatgccgtct acctgcgtga
     6361 ggaccgcacc ctcggctcac cgggccggat gccgttcacc cgcggtcgcg cggtgcgcga
     6421 cgccaccgct ccgtgggacg tccgccagcg gcatgacgac cccgacgtcg ccgtcacccg
     6481 ggctgccgtc ctcgacgacc tcgagcacgg tgtgacctcg gtctggctgc acatcgggga
     6541 cgacgggctc gccgtgggcg atctgcgcga ggcgctcgcg gacgtgcgcc tcgacctcgc
     6601 cccggtcgtc gtgtcgtcgc acacccacca ggtcgaggct gcccaggcgc tgctcgacca
     6661 cgtcggcgac catcccagcg tcgggggcaa cctcggtctc gacccgctgg cggccgcagc
     6721 gaggctcggc gccgagcctg acctcacccc cctcgccgac ctcgtgcggg ctaccgcgac
     6781 ccgtcccggc tggcgcgcca tcacggtcga cgccacggtc ctgcacgacg cgggtgccac
     6841 cgacgtcgac gccctcgccg cagccgtcgc gacgggtgtc gcgtacgtgc ggcacctcga
     6901 ggccgccggc atcgcacccg cgcaggcctt cgaccaggtc gagttccgcg tcggggccac
     6961 cgccgaccag ttcctgaccg ccgccgccct gcgcgccctg cgccgggtgt gggcccgcgt
     7021 cggcgaggca agtggcgtcc ccgaggccag ccgcggcgcg cggacccacg cggtcacgag
     7081 cctgcggatg ttcacccgcg aggacccgtg ggtcaacgtc ctgcgcagca ccctcgccac
     7141 gttcggcgcc tcgatcggtg gcgccgacgc catcaccgtg ctgccgttcg acaccgtgct
     7201 cggcctgccc gagcgcctcg ggcgacggct cgcccgcaac acccagatcc tgctggccga
     7261 cgaggccaac gtcgcccggg tcaccgatcc cgggggtggg tcgtggtacc tcgagtcgct
     7321 caccgatgac gtggccagcg ccgtctgggc ccgcttccag gaggtcgagg cctccggtgg
     7381 cgcggtgcag gcgctcgccg acggtctctt cgcctcctgg gtcgcggatg ccgtcgcgca
     7441 gcgcgaccgt gatctcgcca cccggtcgcg tcccctcacg ggggtctcga tgttcccgct
     7501 ggtcggggaa gtcacccccg accggcgcgc ccgggccgac ctgcccaccg cagcgggtgg
     7561 gttcgccccg cgacgggacg ccacggcata caaggcactt cgtgaccgcg cccgagaact
     7621 cggccagccg gcggtcgtcg tccacaccct cggcacccgc cgggacttcg gtgcacgaca
     7681 ggcgttcgtc accaacctgc tcgccgccgg agggat
//
LOCUS       DAOSOC010000118         7715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9448_length_7715_cov_9.131201, whole genome shotgun sequence.
ACCESSION   DAOSOC010000118 DAOSOC010000000
VERSION     DAOSOC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9448_length_7715_cov_9.131201"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PL014_09260"
     CDS             <1..534
                     /locus_tag="PL014_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT57320.1"
                     /translation="RVGDPMDKNTDVGAINSRKQLDRIEEMVAVGEAEGAQRWDSGCE
                     LPTNGFWFRPTVFSGVSQTHRIAQEEIFGPVVSVLTFRTPAEAVAKANNTPYGLSAGV
                     WTEKGSRILWMADQLRAGVVWANTFNKFDPTSPFGGYKESGYGREGGRHGLEAYVTTA
                     GPLERASGADRSKGASK"
     gene            531..1454
                     /locus_tag="PL014_09265"
     CDS             531..1454
                     /locus_tag="PL014_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT57321.1"
                     /translation="MSARVDVRKTYKLYIGGKFPRSESGRSYEVFATASGSGRGSAAR
                     APKFLANAAMGSRKDARDAVVAARKAQPGWAGATAYNRGQVLYRVAEVMESRRAQLVQ
                     DVRDSEGLSPAAAEATVSAAIDRWVWYAGWADKFTQVLGGLNPVAGPYFDISAPEATG
                     VVAVLAPQRSSLLGLVSVVAPVIVSGNTAVVLTSEDRPLPAITLAECLTTSDVPGGVV
                     NLVTGRTGEMAPWLASHRDVNAIDLTGAAGAEGVEWGALEAAAAENLKRVLRPGGAGA
                     EAVEPDFSATPDLSRLTAFLETKTVWHPKGR"
     gene            1584..2243
                     /locus_tag="PL014_09270"
     CDS             1584..2243
                     /locus_tag="PL014_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT57322.1"
                     /translation="MAHDPTTAPASGSSGSPSRQRVVVLAGPSGSGKSRLASRLNSTH
                     GWPIFRLDDFYRDLDDPALPRSEELGIVDWDHPDSWDADRALAALLDLVDTGRTHTPD
                     YDISTSRAVGHRELTAAPDDLVLAEGIFAAEVVARLESVGILHSAWCVHHRPSVTFVR
                     RLARDLKERRKPPITLVRRGLALMRAEPEIVRRQTDLGALPARATEVERQLRRDVSGV
                     V"
     gene            2276..>2586
                     /locus_tag="PL014_09275"
     CDS             2276..>2586
                     /locus_tag="PL014_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57323.1"
                     /translation="MTENPWVAPGSTPPPEPTRPDPTAPPSDAVAPPPYAPSPPGYAA
                     APPGHAGPPPGYGPPPGYGTPPGYTSPPPGYASPPPGYAVPPAPAYAGLEFRPGIIPL
                     RP"
     assembly_gap    2587..2686
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2792..3430
                     /locus_tag="PL014_09280"
     CDS             2792..3430
                     /locus_tag="PL014_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOT57324.1"
                     /translation="MRLDPPLDPTSPEARTWLENELSKGIYRDDRSLLERLQNWLMEL
                     LSSTGSGSLPAWLIAVAIGIALAVLALVVARSVRAERRMTVDRPHGVLDGPTRTATEH
                     RAEAARALAAGDHATAVVESYRAIARSAVERTLLDDLPGRTAHEVAIALAPAFPASAD
                     RLRDAADTFDAVLYGRRAAAPEAARDLVALDTTLATTRPVLPEVPSQAGAGR"
     gene            3538..4692
                     /locus_tag="PL014_09285"
     CDS             3538..4692
                     /locus_tag="PL014_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOT57325.1"
                     /translation="MGAYALAVTLGLTLIALLALTAQRPGEPLDPQSPRPNGARALVE
                     VLREQGVDVEVVRSVADLEALSPDADTTVVMSNPANLGPGATARLREATTTAGRLVLL
                     GVDNDHLDELGLPVRAFGGSAGDLVARCSSSIARDSDVVSVTESRYLVEDGASGVRTC
                     FALPDPESLTGEPAPGGVYGSGMVELGGTATAPDVVLIGFGSSWSNEYIAENSNAGTA
                     VRALGSSPRLVWYHPGAQDLALAGGEAPQDVWPALTGSALVILSVAIVALALVRGRRL
                     GRLVSEPLPVVVRAIETTESRGRLYRRAGDPGRAAAVLRAATAERLGRRLAVAPGAGP
                     AGLVPAVAVATGRPTEEVAGILIGPAPADDAALIHLAQQLTDLEERAHHP"
     gene            4689..5717
                     /locus_tag="PL014_09290"
     CDS             4689..5717
                     /locus_tag="PL014_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT57326.1"
                     /translation="MTDSPHADAWRPAGAENPVSAENPASAESARAALLAVRAEVAKA
                     VVGQDAAVAGLLVALLCRGHILLEGVPGVAKTLLVRALAASLDIETRRVQFTPDLMPG
                     DLTGSLVFDARESDFTFRPGPVFTNLMLADEINRTPPKTQSAMLEAMEERQVSVDGHP
                     HPLPNPFMVAATQNPVEYEGTYPLPEAQLDRFLLKVVLPLPGRDDEVQVLRRHANGFD
                     PRDLAAAGIRPVASVADLDAGVAAVRAVSISDEVAAYIVDIARATRVSPSLALGVSPR
                     GATALMATSRAWAWLAGRTFVTPDDVKALAHATLAHRLSLRPEAELEGVGVSAVLDSA
                     LGSVPVPR"
     gene            5717..7009
                     /locus_tag="PL014_09295"
     CDS             5717..7009
                     /locus_tag="PL014_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT57327.1"
                     /translation="MAITWRSVVLVLLGLAPVAVWPSGATVRWWLLAVALLIALDVLL
                     ASRPGVLDIVRSAPTQVRLGERTTSTLWITNTSGRGVRGQLRDAWPPSAGAVDAVHDL
                     AVPPHERVRLTTMFAPTRRGDRNADRVTVRLRGPLGLAARQRSFPVPGSFRALHAFPS
                     RRHLPSRLAVLRQLDGRAAVRTRGQGTEFDSLREYVEGDDVRSIDWRATARRQHLVVR
                     TWQPEQHRRVIIVLDTSRTSAGRIGDAPRLDAAMDATLLLTALAGHARDRVQVLAGDR
                     VVHARISGTDRARLLHDTISTLAPVESRLVEADWRALATEVVRLGTHRALVVLITSLE
                     SAAIEEGLLPVLPALAAHHRVVVASVSDPALVAMAATRSTTSEVYDAAAAERTVALRR
                     RTASALEQLGVDVIDVGPDELPVRLADHYLMLKRQGLL"
     gene            7102..7332
                     /locus_tag="PL014_09300"
     CDS             7102..7332
                     /locus_tag="PL014_09300"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOT57328.1"
                     /translation="MPESRVIADHRIMGGVPCVRGTRIPVAVIVGLVAQGRTAQEILA
                     DYPTLTADDVRAALEFAAAAVSERQVPLRTSA"
     gene            7329..7691
                     /locus_tag="PL014_09305"
     CDS             7329..7691
                     /locus_tag="PL014_09305"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOT57329.1"
                     /translation="MKFLLDECLSQRVVSLLEEAGHDVVHVVSSALAGSVDDDVLEAA
                     RAAGRVLVSSDTDFGELLANQGAQLPSLILFRQGNRTPDHQAETLLANLEQVAEDLDR
                     GAIVVFTNDNIRIRALPI"
BASE COUNT         1028 a         2894 c         2625 g         1068 t
ORIGIN      
        1 cgcgtcggtg acccgatgga caagaacacc gacgtcggcg ccatcaacag ccgcaagcag
       61 ctcgaccgca tcgaggagat ggtcgcggtc ggcgaggccg agggggcgca gcgctgggac
      121 tccggctgcg agctgccgac caacggcttc tggttccgcc cgaccgtgtt cagcggggtc
      181 agccagaccc accgcatcgc ccaggaggag atcttcgggc cggtggtctc ggtgctcacc
      241 ttccgcacac ccgccgaggc cgtggccaag gcgaacaaca ccccgtacgg cctgtccgcc
      301 ggggtgtgga ccgagaaggg cagccgcatc ctgtggatgg ccgaccagct gcgcgcgggc
      361 gtggtgtggg ccaacacgtt caacaagttc gacccgacct cgccgttcgg cggctacaag
      421 gagtccggct acggccgcga gggtggccgc cacgggctcg aggcctacgt gacgacggcc
      481 ggcccactcg aacgggccag cggagcggac cgatcgaagg gagcgagcaa gtgagcgcgc
      541 gggtggatgt tcgcaagacg tacaagctct acatcggcgg gaagttcccc cgcagcgaga
      601 gcgggcgttc ctacgaggtg ttcgccacgg catccggctc cggtcgcggg tcagcggcgc
      661 gcgcaccgaa gttcctcgcc aacgccgcca tgggctcgcg caaggacgcc cgggatgccg
      721 tcgtcgcggc tcgcaaggcg cagccgggtt gggcgggggc cacggcatac aaccgcggcc
      781 aggtgctcta ccgggtggcc gaggtcatgg agtcccggcg ggcccagctc gtgcaggacg
      841 tgcgtgacag cgaaggcctg tcccccgccg cggccgaggc gaccgtcagc gcggccatcg
      901 accggtgggt ctggtacgcc gggtgggccg acaagttcac ccaggtgctc ggtgggctca
      961 accccgtcgc ggggccgtac ttcgacatct cggcccccga ggcgaccggt gtggtggccg
     1021 tgctggcgcc gcagcgctcg agcctgctcg gcctggtgtc ggtggtggct ccggtcatcg
     1081 tctccggcaa caccgcggtc gtgctgacca gtgaggaccg cccgctgccc gccatcacgc
     1141 tggccgagtg cctgacgacc tcggacgtgc cgggtggggt ggtcaacctc gtcaccgggc
     1201 gcaccgggga gatggccccg tggctggcct cgcaccgcga cgtcaacgcc atcgacctca
     1261 ccggcgcagc aggtgccgag ggcgtcgagt ggggagcgct cgaggctgcg gccgccgaga
     1321 acctcaagcg ggtgctgcgc ccgggcggtg ctggggccga ggcggtggag cccgacttct
     1381 cggccacccc cgacctgtcg cggctgacgg cgttcctgga gaccaagacg gtctggcacc
     1441 cgaagggccg ctgaccccgc agcactttct cgcccgatgc cagaaccttc agctcggcac
     1501 cacttcattt gtggtgtcaa cctgaaacga gtagcaccgg gcgagaaagt tccgagcggg
     1561 ggttggtcct agactgatca tccgtggccc acgacccgac gaccgcccct gcctccggat
     1621 cctcgggttc accgtcccgc cagcgggtgg tcgtgctcgc cgggccgagc ggatcgggca
     1681 agtcccgcct ggcgtcccgg ctcaacagca cccacgggtg gccgatcttc cggctcgacg
     1741 acttctaccg cgacctcgac gacccggccc tgccccgcag cgaggagctc ggcatcgtcg
     1801 actgggacca ccccgacagc tgggacgccg atcgagccct cgcagcgctc ctcgacctcg
     1861 tcgacaccgg ccgcacccac acccccgact acgacatctc cacgagccgc gccgtcggcc
     1921 accgcgagct cacggccgcc ccggacgacc tcgtcctcgc cgagggcatc ttcgccgccg
     1981 aggtcgtcgc gcggctggag tcggtcggca tcctgcacag cgcgtggtgc gtgcaccacc
     2041 ggccgtcggt gaccttcgtg cgacgcctgg ctcgcgacct caaggagcgt cgcaagcccc
     2101 cgatcacgct cgtgcgccgt ggcctagcgc tcatgcgggc cgaacccgag atcgtgcgcc
     2161 gccagaccga cctcggcgcc ctgcccgccc gcgccaccga ggtcgagcgc cagttgagaa
     2221 gggacgtcag cggcgtcgtc tagggtgatc cgatgggtga cggaggggac gcacgatgac
     2281 ggagaatcca tgggtggcac cgggctcgac gccgcctccg gagccgacgc gccctgaccc
     2341 cacggcaccg ccgtcggatg ccgtcgcgcc cccgccgtac gcaccctcac ctcccgggta
     2401 cgccgccgca ccaccgggcc atgccggccc accaccgggt tacggcccac caccgggtta
     2461 cggcacgcca ccggggtaca cctccccgcc acccgggtac gcatcgccgc caccggggta
     2521 cgccgtgccg cctgcgccgg cctatgcggg cctggagttc cggcccggga tcatcccgtt
     2581 gcgcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcga ccagcgcatc
     2701 cgccgggaag ggctggacgt ccagctcatc cacaccgccc agcacggtgg gccgcccccg
     2761 tggcccagcg ccgtccagga ccgctgagcc cgtgcgcctc gatccaccgc tcgaccccac
     2821 gtcccccgag gcgcggacct ggctcgagaa cgagctgagc aagggcatct accgcgacga
     2881 ccgcagcctg ctggaacgcc tgcagaactg gctcatggag ctgttgtcca gcaccgggtc
     2941 aggcagcctg cccgcgtggt tgatcgccgt ggcgatcggc atcgccctgg ccgtgctcgc
     3001 cctcgtggtg gcgcgttcgg tgcgggccga gcgtcgcatg acggtcgacc gaccccacgg
     3061 cgtcctcgac ggcccgacgc gcacggccac cgaacaccgg gccgaggctg cccgggcgct
     3121 ggcagcgggc gatcacgcca cggcagtcgt cgagtcctac cgcgccattg cccgctcggc
     3181 cgtcgagcgc accctgctcg acgacctgcc cggccgcacc gcccacgagg tggcgatcgc
     3241 gctggcaccc gccttcccgg catccgccga tcgcctgcgc gatgctgccg acaccttcga
     3301 cgccgtgctc tatgggcgcc gggccgccgc tcccgaggcc gctcgcgacc tggtcgccct
     3361 cgacaccacg ctggccacca cccggcccgt gctccccgag gtgccctcgc aggccggggc
     3421 cggccgatga ccgccggcgc ccccaccacc cagtccgcgc ccgccgacgt gctctcggct
     3481 ggcgacgccg gtgggatgcc gcgtgcagcg gcgccgcgcg cacggcgacg tcgtcgaatg
     3541 ggcgcctacg ccctcgccgt gacgctcggt ctgaccctca tcgccctcct ggccctcacg
     3601 gcccagcggc cgggagagcc gctggacccg cagagcccac gcccgaacgg ggcacgagcg
     3661 ctcgtcgagg tgctgcgcga gcagggcgtc gacgtcgagg tcgtccgctc ggtcgcagac
     3721 ctcgaggcgc tcagccccga cgccgacacc acggtggtga tgagcaaccc cgccaacctc
     3781 ggtccgggag ccaccgcacg tctgcgcgag gcgacgacca cggccggtcg gctcgtcctg
     3841 ctcggggtgg acaacgacca cctcgacgag ctcgggctgc ccgtgcgagc gttcggcggt
     3901 tctgccggcg acctcgtggc gcgctgctcc tcgagcattg cccgtgacag tgacgtcgtg
     3961 tcggtcaccg agagccgcta cctcgtcgag gacggagcga gcggggtgcg gacctgcttc
     4021 gccctgcccg accccgagag cctgaccggc gagcccgccc ccggtggtgt ctacggctcc
     4081 ggcatggtcg agctgggggg caccgcgaca gcaccggacg tcgtcctgat cgggttcggg
     4141 tcctcgtgga gcaacgagta catcgccgag aacagcaacg ccggcaccgc ggtgcgcgcg
     4201 ctcggctcgt ccccgcgcct ggtctggtac caccccggag cccaggatct cgcgctcgcc
     4261 ggaggcgagg ccccgcagga cgtgtggccg gcgttgaccg gttctgccct ggtcatcctc
     4321 tcggtcgcga tcgtggccct cgccctggtg cgcggacgtc gcctcgggcg tctcgtgagc
     4381 gaaccactgc ccgtcgtcgt gcgggccatc gagacgaccg agagccgcgg acggttgtac
     4441 cgccgcgccg gcgacccggg ccgggccgcc gccgtgctgc gtgccgccac ggctgaacgc
     4501 ctcggccgtc ggctcgccgt cgcccccggc gccggcccgg caggcctggt tcctgccgtg
     4561 gccgtcgcga ccggtcggcc caccgaagag gtcgctggca tcctgatcgg gcctgcgccc
     4621 gcggacgacg cggccctgat ccacctcgcc cagcagctca ccgacctcga ggagagggcc
     4681 caccacccat gaccgactca ccacacgccg acgcctggcg cccagcgggc gccgagaacc
     4741 ccgtgagcgc cgagaaccct gccagcgccg agagcgcccg ggcggccctg ctcgccgtgc
     4801 gcgccgaggt cgccaaggcc gtcgtcggtc aggatgccgc ggtcgccggg ctgctcgtcg
     4861 ccctgctgtg tcgcggccac atcctgctcg agggggtgcc tggtgtcgcc aagacgctgc
     4921 tggtgcgcgc cctcgccgcc tcgctcgaca tcgagacgcg gcgggtgcag ttcacccctg
     4981 acctcatgcc cggtgacctc accggttcgc tggtcttcga cgcccgcgag agcgacttca
     5041 ccttccgccc ggggccggtc ttcaccaacc tcatgctcgc cgacgagatc aaccgcactc
     5101 ccccgaagac gcagtcggcg atgctcgagg cgatggagga gcgccaggtc agcgtcgacg
     5161 gccaccccca cccgctgccg aacccgttca tggtggccgc gacccagaac cccgtcgagt
     5221 acgagggcac ctacccgctc cccgaggcgc agctcgaccg cttcctgctc aaggtcgtgc
     5281 tgcccctgcc cggccgcgac gacgaggtgc aggtgctgcg gcgccacgcg aacgggttcg
     5341 acccacgcga cctggccgcg gccggcatcc gcccggtcgc cagcgtcgcc gacctcgatg
     5401 ccggtgtcgc agcggtgcgc gcggtctcca tctccgacga ggtggctgcc tacatcgtcg
     5461 acatcgcccg ggcgacgcgg gtctcgccgt cgctggccct gggcgtgtcc ccccgtggtg
     5521 ccacggcgct catggcgacg agccgggcgt gggcctggct ggccgggcgc acgttcgtca
     5581 cccccgacga cgtcaaggcc ctcgcgcacg cgacgctggc gcaccggctg tcgctgcgcc
     5641 ccgaggccga gctcgagggc gtcggcgtca gtgcggtgct cgacagtgcc ctcggttcgg
     5701 tgcccgtccc ccgctgatgg cgatcacctg gcgctcggtc gtgctggtcc tgctggggct
     5761 ggcaccggtc gccgtctggc cgagcggcgc gacggtgcgc tggtggctgc tcgccgtcgc
     5821 cctgctcatc gcgctcgacg tgctgctggc gagccgcccg ggggtgctcg acatcgtgcg
     5881 cagcgcgccg acccaggttc gactcggcga acggacgacc agcaccctgt ggatcaccaa
     5941 cacctccggc cgcggggttc gtggtcagct gcgggacgcg tggcctccgt cggcaggggc
     6001 ggtggatgcc gtgcacgacc tcgcggtgcc gcctcacgaa cgggttcggt tgaccaccat
     6061 gttcgccccg acccggcgcg gtgatcgcaa cgccgaccgg gtgacggttc ggctgcgcgg
     6121 cccgctcggg ctggcggccc ggcagcgctc gttccccgtg cccggctcgt tccgagccct
     6181 gcatgccttc ccgtcgcggc gccacctccc gagccggttg gcggtccttc gccagctgga
     6241 cggccgggcg gcggtgcgga cccgcggcca gggcaccgag ttcgacagcc tgcgcgagta
     6301 cgtggagggc gacgacgtgc gctcgatcga ctggcgggcc acggctcggc gccagcacct
     6361 cgtcgtgcgc acctggcaac ccgagcagca ccggcgggtc atcatcgtgc tcgatacctc
     6421 acgcacgagc gccgggcgca tcggtgacgc accccgcctg gatgccgcga tggatgccac
     6481 gctgctgctc actgcgctgg ccggtcacgc tcgcgaccgg gtgcaggtgc tggccggtga
     6541 ccgggtcgtc cacgcccgga tcagcggcac cgaccgcgcc cggttgctgc acgacacgat
     6601 cagcaccctc gccccggtcg aatcccggct ggtcgaggcc gactggcgag ccctggccac
     6661 cgaggtggtg cgactgggca cccaccgggc gctcgtcgtg ctcatcacct cgctggagtc
     6721 cgcggccatc gaggaagggt tgcttcccgt gctgcctgcc ctcgcggccc accaccgggt
     6781 ggtcgtcgcc tcggtgagcg acccggcgct cgtggcgatg gccgcaacgc gctccacgac
     6841 gtccgaggtc tatgacgcag ccgccgcgga acgcacggtg gcgctgcgcc gccgcacggc
     6901 atccgcgctg gagcagttgg gggtcgacgt catcgacgtc gggcccgatg aactgcccgt
     6961 gcgcctggcc gaccactacc tgatgctcaa gcgccaaggc ctgctctgac gatcggggtc
     7021 catcgcttcg gaccactggg gcgaattgat gcggaacccg ggcccggcgc acgggcaccc
     7081 ctcgggccta cactcaatct catgccagag tcccgagtca tcgccgacca ccgcatcatg
     7141 ggcggggtcc cttgcgtgcg tggaactcgg atcccggtcg ccgtgatcgt cgggctggtg
     7201 gctcagggtc gcacggccca ggagatcctc gctgactacc ccaccttgac cgctgatgac
     7261 gtccgcgcgg ccttggagtt cgccgcagca gcggtcagcg agcggcaggt tccgctgcgc
     7321 acctcggcgt gaagttcctt ctcgacgagt gcctcagcca gcgtgtggtg tcgctactcg
     7381 aggaggccgg ccatgatgtc gtgcacgtcg tgagtagcgc acttgccgga agcgtcgacg
     7441 atgacgtctt ggaagcagcc cgcgccgcgg gtcgggtcct ggtctcctcg gacaccgact
     7501 tcggtgaatt gctggccaat cagggtgctc aacttccgag cctgatcctg ttccggcagg
     7561 gtaaccgaac accagaccat caagccgaga ccctgctggc gaacctcgag caggttgccg
     7621 aagacctgga tcgcggcgcc atcgtcgtct tcaccaacga caacatccgg attcgggccc
     7681 tgccgatcta gagcgggccg ggtgcactgg cggcg
//
LOCUS       DAOSOC010000119         7709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9457_length_7709_cov_7.048079, whole genome shotgun sequence.
ACCESSION   DAOSOC010000119 DAOSOC010000000
VERSION     DAOSOC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9457_length_7709_cov_7.048079"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PL014_09310"
     CDS             <1..327
                     /locus_tag="PL014_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HOT57330.1"
                     /translation="PSDYLWDTVSIGTLVAFSVVALGVLVLRRTHADLDRPFRIPGYP
                     VTPLLTIAACVWILIGLPMTTWVIFLAWLAIVMAFYLAYGRRNATLNTYVDPEEIAEP
                     RGKEDQ"
     gene            324..1211
                     /locus_tag="PL014_09315"
     CDS             324..1211
                     /locus_tag="PL014_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT57331.1"
                     /translation="MTILLAFSPHQDDTGAVELACQLARSNNDSVHAVTVVPQAWPTA
                     VAGDTDRDFKLWAQEEGEWSASAARDVLAEHPDVVSEASWCAGRSVPQALLDRAAELD
                     AGLIVVGSGDEGPHGKVFLTSKTDRLLHSSGVPVAIAPQGYWSGPTGRVTRATLGFRG
                     DDATWSLLDRAAEICRRVDASMRLVTYAVRGRTMRPSTVSGAEEMVHQQWIRQATAEL
                     KEAVEHLHSLGFTDDQVSSEVAVGSSWGRAMDRLEWHRGDVLVVGSSSTSSLLQRVFL
                     GSSAAKIVRNSPVPVLVVP"
     gene            complement(1232..2257)
                     /locus_tag="PL014_09320"
     CDS             complement(1232..2257)
                     /locus_tag="PL014_09320"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HOT57332.1"
                     /translation="MRLHEIPRHPLTFGPSPLQHLRRLSNHLGGAQVWAKREDVNSGL
                     AFGGNKVRKLEYIVPDVLASGADTLVSIGGYQSNHTRAVAAVAAHLGLKARLVQEKWV
                     PWDDPVNDKVGNILLSRMMGADSRLDPAGFDIGIRDSWKDALREVEESGGTAYPIPAG
                     ASEHPLGGVGFANWAFEVAEQEETLGILFDTIVVCTVTCSTHAGMIAGFAALEDLTGV
                     RRRVIGIDASATLAKTRDQLERIARHTAEVIDLGRDLRDDEFTLLDGWAGDLYGIPVD
                     STMAAMRLGAELEAMITDPVYEGKSLAGLIDLVRERDIPADSTVLFAHLGGQPALNAY
                     HSLFPGT"
     gene            complement(2276..2938)
                     /locus_tag="PL014_09325"
     CDS             complement(2276..2938)
                     /locus_tag="PL014_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOT57333.1"
                     /translation="MSSSKRPRLQRHDVVEAAVALADEVGIDALSMRTLAQRLDVVPM
                     ALYKHVANKDELLDAMVDHVVGEIEPAGPDENWRHGIRSRILSSRAALLRHPWAPGLI
                     ETRTQASPVVLDHMEAIAASFRAGGFSVDLTHRAMHAIGSRMWGFTQEVFPTQEPAQD
                     ETAREQQFDEWMKRYPRIVEMATEIGGSGGCDAHQEFEFALDLILDGLERIHRETVRD
                     DG"
     gene            3012..3731
                     /locus_tag="PL014_09330"
     CDS             3012..3731
                     /locus_tag="PL014_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HOT57334.1"
                     /translation="MTPTRRSALAAGLLYLATIVSSIPALALIQPILTQPEYVLGDGP
                     QTRVVFGIVLDLVNVAAAIGTAVALYPIIRRRHQTLAIGFVASRLLEGATLGIGALAL
                     VAVVTLRRDPGTASDETLVAASQSLVSLRDATFLLAPGLLASVNAALLGTALYRARLV
                     PRGIPLLGLIGAPLLFASTLATLVGVFEQLSPWSVVATLPIFLWELSLGLHLTLRGVT
                     ELDHDDSAPAPRPDRTPADAH"
     gene            3676..4587
                     /locus_tag="PL014_09335"
     CDS             3676..4587
                     /locus_tag="PL014_09335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT57335.1"
                     /translation="MTTPPPPPGLTARPLTPTDLDEVYAVYAADEVADAGHLAIEPED
                     LAGDWSRPSFDLAHDSIGLFENGHVVAAAEVTRGGTRAEGAVHPDVRGRGIGSWLVDW
                     TEARARELGAATVGQTTPEGSVAQRLFLNRGYRLGHTSWVLALPAGAEIEQRPLPPGY
                     TLGHCPDARRERAAYEVIQQAFDEWEGRERESYADWAATTVRRPGTQPWQLRIVEHEG
                     EVVGASFTILDMQATGYLYQLAVDPRHRGKGLAQAMLADAFERAREEGATHSELSTDS
                     RTGALDLYRKVGMEVTQTWTHLVTDLR"
     gene            complement(4594..4848)
                     /locus_tag="PL014_09340"
     CDS             complement(4594..4848)
                     /locus_tag="PL014_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HOT57336.1"
                     /translation="MTSEDEFHAAVEQVKTLVVDPGNSVKLQLYALYKQATEGDVTGK
                     RPGFTNPVGRAKYDAWAEQEGLSAEEARSEYVTLVHSLMA"
     gene            complement(4908..>5912)
                     /locus_tag="PL014_09345"
     CDS             complement(4908..>5912)
                     /locus_tag="PL014_09345"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT57337.1"
                     /translation="QVGNVMRTLLHERNFPVEQIRYFASARSAGSTLPWGDTEITVED
                     TATADFSGLDIALFSNGGSTSKEWAPRVAAAGAVVIDNSSAWRKDPDVPLVVSEVNPE
                     DLDVIPKGIVANPNCTTMAAMPVLKPLHDLAGLVRLHVSSYQAVSGSGGVGLTELDSQ
                     LRGGYAAGDPKELALDGSALTLPAPQVYAVGVGFNVVPLAGSIVDDGSLETDEEQKLR
                     NESRKILHVPDLRVSGTCVRVPVFSGHSLAIHAEFAGDISVEQALEALGQAPGVIVTD
                     VPNPLMATGRDEVFVGRVRADQAAPEGKGLNLFVVGDNLRKGAALNAVQVAEELLKRR
                     "
     assembly_gap    5913..6012
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6084..6242)
                     /locus_tag="PL014_09350"
     CDS             complement(6084..6242)
                     /locus_tag="PL014_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57338.1"
                     /translation="MIPHDFVVFGDAAKGVASVEDGLRLVWPVVADVFAQVWDADGPP
                     SAADLFAG"
     gene            complement(6461..7651)
                     /locus_tag="PL014_09355"
     CDS             complement(6461..7651)
                     /locus_tag="PL014_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HOT57339.1"
                     /translation="MAKSPRVREQHIAVFGERGSGKTVLVSSFFGPTQEGSYSNELWN
                     LIADETGQGNRLYQNYLGMRDRATAPATTKFASTTYYFTLKLKSGDNSASKRRPFDAL
                     RLAWHDYPGEWFEETPSSDEEAQRRVDTFRSLLRSDVAVFLVDGQKLVDYQGEEERYL
                     KSLFTNFRQGLLRLKDDLLVDDARLVEFPRIWILALSKADLFPDWDVYAFRDLVIGKA
                     AGDIDRLRETIEEFVDTPQALSVGEDFMLLSSAKFELTPTGSELGEIDVTQRVGLDLI
                     MPVASLLPLERRVLWNERLEIPRKVLDAMADGAEDIAAALKGPLGIGLVKVLSKLPKG
                     GRVASAAAVPVFLAAADAAGPKLKELNVQARANLDYLTATLTQFKLDLDQGVKDRLLI
                     RSLK"
BASE COUNT         1159 a         2602 c         2636 g         1212 t
ORIGIN      
        1 ccctcggact acctgtggga caccgtctcg atcggcacgc tcgtcgcgtt ctcggtcgta
       61 gccctgggcg tcctcgtcct gcgtcgcacg cacgccgacc tcgatcgccc cttccgcatt
      121 ccgggctacc ccgtgacgcc cctgctgacc atcgcggcct gcgtgtggat cctcatcggt
      181 ctgccgatga cgacgtgggt catcttcctc gcctggctcg ccatcgtgat ggcgttctac
      241 ctcgcgtacg gtcgtcgcaa cgcgacactg aacacgtacg tcgaccccga ggagatcgcc
      301 gagccgcgcg gtaaggagga ccagtgacca tcctgctggc gttcagccct caccaggacg
      361 acaccggggc agtggagctc gcgtgccagc tcgcccggtc caacaacgac tcggtgcacg
      421 ccgtcacggt ggtgccgcag gcctggccga cggccgtcgc cggtgacacc gatcgcgact
      481 tcaagctgtg ggcgcaagag gaaggtgagt ggtcggcatc cgctgcccgt gacgtgctcg
      541 cagagcaccc ggacgtcgtc agcgaggcca gctggtgcgc ggggcgttcc gttccccaag
      601 ccctgctcga ccgggcggcc gagctcgacg caggcctcat cgtcgtcggg tccggcgacg
      661 aggggccgca cggcaaggtc ttcctcacga gcaagaccga ccggctgctg cactcgtccg
      721 gggtgccggt ggcgatcgcg ccgcaggggt actggagcgg gcctacgggg cgggtcaccc
      781 gcgccaccct cgggttccgt ggcgatgacg ccacgtggtc gctgctcgac cgcgccgccg
      841 agatctgccg ccgcgtcgat gcctcgatgc gcctggtcac ctacgccgtg cgcggtcgca
      901 ccatgcggcc cagcaccgtc agcggagccg aggagatggt gcaccagcag tggatccgcc
      961 aggccaccgc cgagctcaag gaggcagtgg agcacctgca ctcgctgggg ttcaccgatg
     1021 accaggtgtc cagcgaggtg gccgtggggt cgtcgtgggg cagggccatg gatcgcctcg
     1081 agtggcaccg cggcgacgtg ctcgtcgtgg ggtcgtcgtc gacgtcgtcg ctgctccagc
     1141 gggtcttcct cgggtcgagc gccgcgaaga tcgtgcggaa ctcccccgtg ccggtgctgg
     1201 tcgttccgta gccgctaccc ctgctgccgg ctcaggtgcc cgggaagagt gagtggtagg
     1261 cgttgagcgc gggctgccca ccgaggtggg cgaagagcac cgtcgagtcg gcggggatgt
     1321 cgcgctcgcg cacgaggtcg atgagcccgg ccagtgactt gccctcgtac acggggtcgg
     1381 tgatcatcgc ctcgagctcg gcgcccaggc gcatggccgc catcgtggag tcgacgggga
     1441 tgccgtagag gtcgccggcc cagccgtcga gcagggtgaa ctcgtcgtcg cgcaggtcgc
     1501 gcccgaggtc gatgacctcg gccgtgtgcc gggcgatgcg ctcgagctgg tcgcgagtct
     1561 tggccagggt ggccgaggcg tcgatcccga tgacccgacg gcgcaccccg gtgaggtcct
     1621 cgagcgcggc gaacccggcg atcatcccgg cgtgggtcga gcaggtgacg gtgcacacga
     1681 cgatggtgtc gaagaggatg ccgagcgtct cctcctgctc ggcgacctcg aacgcccagt
     1741 tcgcgaagcc gaccccgccg agcgggtgct cggaggcacc ggcgggaatc gggtaggcgg
     1801 tgccgccgct ctcctcgacc tcgcgcaggg cgtccttcca cgagtcgcgg atgccgatgt
     1861 cgaacccggc cgggtccagg cgcgagtcgg cgcccatcat ccgggagagc aggatgttgc
     1921 cgaccttgtc gttgaccggg tcgtcccagg gcacccactt ctcctgcacg aggcgagcct
     1981 tgaggccgag gtgggccgcg accgcggcga cggctcgggt gtggttggac tggtagccac
     2041 cgatggacac gagagtgtcg gcgccactgg cgaggacgtc ggggacgatg tactcgagct
     2101 tgcgcacctt gttgccaccg aacgcgaggc cgctgttgac gtcctcacgc ttggcccaca
     2161 cctgggcacc cccgaggtgg ttgctcagcc ggcgcaggtg ctgaagaggg ctggggccga
     2221 aggtgagcgg gtggcgaggg atttcgtgca ggcgcatgga accacggtag ccacctcagc
     2281 cgtcgtcgcg caccgtctcg cggtggatcc gctcgaggcc gtcgaggatg aggtcgaggg
     2341 cgaactcgaa ctcctggtgg gcgtcgcacc caccactacc gccgatctcg gtggccatct
     2401 cgacgatgcg ggggtagcgc ttcatccact cgtcgaactg ctgctcacgg gccgtctcgt
     2461 cctgagccgg ctcctgtgtg gggaagacct cctgggtgaa cccccacatc cgggaaccga
     2521 tggcgtgcat ggcgcggtgc gtgaggtcga ccgagaaccc cccggcacga aacgacgcgg
     2581 cgatcgcctc catgtggtcg agtacgacgg gagaggcctg ggtgcgggtc tcgatgagcc
     2641 cgggcgccca cggatggcgc agcagcgccg ccctcgacga caaaatccgc gatcggatgc
     2701 cgtgccgcca gttctcgtcc gggccggcgg gttcgatctc gcccacgaca tggtcgacca
     2761 tcgcgtcgag gagctcgtcc ttgttggcca cgtgcttgta gagcgccatg gggacgacgt
     2821 cgaggcgctg ggccagggtg cgcatgctca gcgcgtcgat gccgacctcg tcggcgaggg
     2881 ccaccgcggc ctcgacgacg tcgtggcgct ggagccgggg gcgcttgctg cttgacacgc
     2941 gtacaccgta cacctatggt catcgcattc tcaggtgtac gtcgtacacg tttatgcaga
     3001 aggagccatc catgaccccc acccgacgct cagccctggc cgccgggctg ctctacctcg
     3061 cgacgatcgt gtcctcgatc cccgcactgg ccctcatcca gccgatcctc acccagcccg
     3121 agtacgtcct cggcgacggg ccgcagaccc gggtggtgtt cggcatcgtc ctcgacctcg
     3181 tcaacgtcgc cgcggcgatc ggcacggcgg tggcgctcta cccgatcatc cgtcggcgac
     3241 accagacgct tgccatcggc ttcgtcgcct cgcggctgct cgagggcgcc accctgggga
     3301 tcggcgcgct ggcgctggtc gcggtggtga ccttgcgacg cgaccccggc acggcatccg
     3361 atgaaaccct cgtcgcagca tcccagtcgc tcgtctcctt gcgcgacgcc acgttcctgc
     3421 tggcgccggg gctcctcgcc tcggtcaacg ccgccctgct cggcaccgcc ctgtaccgcg
     3481 cccggctcgt gccgcgcggc atcccgctgc tcggcctgat cggcgcaccg ctgctgttcg
     3541 cgagcacgct ggcgacgctc gtcggggtgt tcgagcagct gtcgccgtgg agtgtcgtcg
     3601 cgacgctgcc gatcttcctg tgggagctca gcctggggct gcacctgacc ttgcgcggag
     3661 tgacagagtt ggaccatgac gactccgccc ccgccccccg gcctgaccgc acgcccgctg
     3721 acgcccactg acctcgacga ggtgtatgcc gtctacgcgg ccgacgaggt cgcggatgcc
     3781 ggccacctcg ccatcgagcc cgaggacctc gccggcgact ggtcgcgccc gagcttcgac
     3841 ctggcgcacg acagcatcgg gctcttcgag aacgggcacg tcgtcgcggc tgccgaggtc
     3901 actcgcggcg gcacccgggc cgagggcgcc gtgcaccccg acgtgcgcgg tcgcggcatc
     3961 ggctcgtggc tcgtggactg gaccgaggcc cgggcccgcg agctgggtgc cgccacggtg
     4021 ggccagacga cacccgaggg ctcggtcgcg cagcggctgt tcctgaaccg cgggtaccgc
     4081 ctcggacaca cctcgtgggt gctggccctg ccggccggcg cggagatcga gcagcgcccg
     4141 ctcccgccgg gatacacgct cggtcactgt cccgacgcgc ggcgggagcg ggccgcctac
     4201 gaggtgatcc agcaggcgtt cgacgagtgg gaggggcgcg agcgggagag ctacgcagac
     4261 tgggcggcga cgacggtgcg ccgacccggc acccagccct ggcagctgcg gatcgtcgag
     4321 cacgagggtg aggtcgtcgg ggcctcgttc acgatcctgg acatgcaggc cacgggctac
     4381 ctctaccagc tcgcggtgga ccctcggcac cgcgggaagg gactggccca ggcgatgctg
     4441 gccgacgcct tcgagcgggc ccgcgaggag ggcgcgacgc acagcgagct gtcgaccgac
     4501 tcacgcaccg gcgcgctcga cctctaccgc aaggtcggca tggaggtcac gcagacctgg
     4561 acgcacctgg tgaccgacct gcgctgaggc gcctcaggcc atgagcgagt gcacgagcgt
     4621 gacgtactcg ctgcgtgcct cctcggcgct caggccctcc tgctcggccc aggcgtcgta
     4681 cttcgcccgg ccgacggggt tggtgaagcc ggggcgcttg cccgtgacgt caccctcggt
     4741 ggcctgcttg tagagggcgt agagctgaag cttgacgctg ttgcccgggt cgaccacgag
     4801 ggtcttgacc tgctcgactg cggcgtggaa ctcgtcctcg gaagtcatga cgcaactcta
     4861 ggggtcaacc ccgtggaggt caggggtttc gcggctcagc ggcgtggtca gcgacgcttg
     4921 agcagttcct ccgcgacctg gaccgcattc agggccgcac ccttgcgcag gttgtcaccg
     4981 acgacgaaca ggttcaggcc cttgccctcg ggggcggcct ggtcggcccg cacgcgaccg
     5041 acgaagacct cgtcgcggcc ggtggccatg agcgggttcg ggacgtcggt gacgatgacg
     5101 ccgggcgcct ggccgagcgc ctcgagggcc tgctcgacgc tgatgtcacc ggcgaactcg
     5161 gcgtggatcg cgaggctgtg gccggagaac accggcacgc gcacgcaggt gcccgagacg
     5221 cgcaggtcgg ggacgtgcag gatcttgcgc gactcgttgc gcagcttctg ctcctcgtcg
     5281 gtctccagcg agccgtcgtc gacgatcgag ccggccagcg gcacgacgtt gaacccgacg
     5341 cccacggcat acacctgggg cgcgggaagg gtgagggccg acccgtcgag cgcgagctcc
     5401 ttggggtcgc ctgcggcgta gccgccgcgc agctggctgt cgagctcggt gaggccgaca
     5461 ccaccggagc cggagacggc ctggtagctg ctgacgtgca gacgcacgag cccggcgagg
     5521 tcgtgcagcg gcttgagcac cggcatcgcg gccatcgtcg tgcagttggg gttggcgacg
     5581 atccccttgg ggatgacgtc gaggtcttcc gggttgacct cggagacgac gagcgggacg
     5641 tcggggtcct tgcgccacgc gctggagttg tcgatgacga cggcgccggc agcggcgacg
     5701 cggggcgccc actccttcga cgtcgaaccc ccgttggaga acagggcgat gtcgaggccg
     5761 gagaagtccg cggtcgcggt gtcctcgacg gtgatctcgg tgtcgcccca cggcaacgtc
     5821 gagcccgcgg aacgcgcgga cgcgaagtag cggatctgct cgaccgggaa gttccgctcg
     5881 tgcagcaggg tgcgcatgac gttgccgacc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nngctgaact cttctactcc agcctgatca ccagcatcga ccccgaagcc
     6061 gtgacgacat tcgagttccg gggttagcca gcgaacaggt cggccgcaga cggtgggcca
     6121 tcggcatccc acacctgggc gaagacgtcc gcgacgactg gccagacaag cctcagtccg
     6181 tcctccacgg atgcgacccc ctttgccgcg tcaccgaaca ccacgaagtc gtgcgggatc
     6241 acccgccccg cggtatctcg ccggcccaag gggtctgaga atcgcagcgc caccaactcg
     6301 ccctcccggt ggcaaccctg cgactcgtcc cccagaacga caaaagcccg ttcgtaggtt
     6361 gccaggggat cgggaagacc cgcagtcagc aagaagcgga agccccaacg acgcccacac
     6421 gtggcccaga tgagcgacgc ggcggcagct ccgggctcag tcacttcaga ctccggatga
     6481 gcaagcgatc cttgactcct tggtcaaggt ccaacttgaa ctgggtcagg gtcgccgtca
     6541 ggtagtcaag gttcgcgcgc gcttggacgt tgagctcctt gagtttgggg cccgctgcgt
     6601 cagccgccgc gaggaacaca ggcacggcag ccgcgctggc aacacggccg cccttcggta
     6661 gctttgacaa caccttgacc aagccgatcc ccaggggccc cttcagcgct gccgcgatgt
     6721 cctcggcacc atcggccatg gcgtcgagca ccttccgcgg gatctccaag cgttcgttcc
     6781 acagaacccg tcgctctagc ggcaacagcg acgccaccgg catgatcagg tcaaggccga
     6841 cgcgctgagt cacgtcaatc tccccgagct ccgagcccgt cggagtcagt tcgaacttcg
     6901 ccgacgacag caacatgaag tcctcgccga cggacaaggc ctggggcgtg tccacgaact
     6961 cctcgatggt ttcgcgaagg cggtcgatgt cgccggcggc cttaccgatg acgaggtcac
     7021 ggaaggcgta gacgtcccag tctgggaaca ggtccgcctt cgacagcgcc agaatccaga
     7081 tccgcgggaa ctcgaccaat cgggcgtcgt cgacgagcag atcatccttg aggcgcagca
     7141 ggccctgacg gaaattggtg aacagcgact tgaggtatcg ctcttcctcc ccttggtagt
     7201 cgactagctt ctggccgtcg acgagaaaga cggccacatc tgaacggagc agggatcgga
     7261 aggtgtccac gcgccgctgc gcttcctcgt cactgctcgg cgtctcctcg aaccactccc
     7321 cggggtagtc gtgccacgcg agtcgcaacg cgtcgaaggg gcgccgcttt gaggctgagt
     7381 tgtcaccact cttcagcttg agcgtgaagt agtacgtggt gctcgcgaac ttcgtcgtgg
     7441 caggggcggt ggcacggtct cgcatgccga ggtagttctg atacagccga ttgccttggc
     7501 cggtctcatc ggcgatgagg ttccacagct cgttggagta cgacccttct tgagttggtc
     7561 cgaagaagga cgacaccaag acggtcttgc ctgagcccct ttccccaaaa accgcgatgt
     7621 gctgctctcg gacccgcggg gatttcgcca tgccgcgaag gtacccgatc gagtgtcagg
     7681 gtgcggtgag caggtggcgc tgactccac
//
LOCUS       DAOSOC010000120         7708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9461_length_7708_cov_7.770940, whole genome shotgun sequence.
ACCESSION   DAOSOC010000120 DAOSOC010000000
VERSION     DAOSOC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9461_length_7708_cov_7.770940"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="PL014_09360"
     CDS             complement(<1..1313)
                     /locus_tag="PL014_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT57340.1"
                     /translation="MSAVVDTPAAPPTPQLPTEVVTHTPVQDVALPGGAGILALITLD
                     NGLDHTRPNTFGPASIAELQATVDGLKARAAAGEIQAVGITGKPFIFAVGADLSGVPY
                     VTDREQAVSIARAGHAAFASIMDLPVPTFAFINGAAMGGGVEIALACDYRTVSAGVPA
                     FALPETFLGLVPGWGGCYLLPNLIGPADALTVIVANPLSQNRMLKAKEVAALGIADVL
                     LEPADFIEDSLRWAAGVVSGAVPVQRRDVDRDESAWDAACDGARSVVLAKTAGRAPGA
                     MRAVELVRAARTATREEAFAAEDAALGDLIMGDELRAGLYAFDLVQKRAKRPAGAPDK
                     ALARPVTKVGIVGAGLMASQLALLFARRLGVPVVMTDLDEDRVAKGVGWVHAEIASLK
                     DKRRVSPDKASFLTGLVTGSTSKDGFADADFVIEAVFEEMAVKQQV"
     gene            complement(1315..2526)
                     /locus_tag="PL014_09365"
     CDS             complement(1315..2526)
                     /locus_tag="PL014_09365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT57341.1"
                     /translation="MPRTLQEVVFVDGVRTPFGKAGEKGIYAQTRADDLVIKCMRELL
                     RRHPELPKERVEEVAIAATTQIGDQGLTLGRLAGLLAGLPKTTPGYSVDRMCAGAMTA
                     VTNVASSIGFGAVDVAIAGGVEHMGRHPMGEGIDPNPRIIGERIVDESALVMGKTAEN
                     LHDRFPHLTKERCDAYAVNSQRKLAAAYAAGKVQPDLVPVATRHVEKGWGLATVDEPP
                     RPGTTMEELAGLKTPFRPHGNVTAGNAAGLNDGATACILASEAAAAELGLPVRMRLVS
                     YAFVGVEPEVMGIGPIPAAEKALRQAGLTIEDIGLFELNEAFAVQVLAFLDHYGIADD
                     DERVNPWGGAIAIGHPLASSGVRLMTQLAHHFEENPQVRYGLVAMCIGIGMGGCVIWE
                     NPHHADYGKEN"
     gene            complement(2713..3987)
                     /locus_tag="PL014_09370"
     CDS             complement(2713..3987)
                     /locus_tag="PL014_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOT57342.1"
                     /translation="MITETTTPPPPGDQHDRSSSDHRVPEAAPVTLVPLIEPSGGVPH
                     VVETERELARAAKAIAAGEGPVGIDAERASGYRYGQRAYLVQVRREGAGTWLIDPMGC
                     PDLSPLGEAIGAAEWILHAATQDLACLAEVGLRPVQLFDTELAGRILGLPRVGLAAVV
                     EHYLGLGLAKEHSAVDWSTRPLPEPWLRYAALDVEVLDELRNLMGVDLAAQGKAEWAR
                     QEFAALLQWAPTERVDPWRRTSGINTLRSRRSVAIVRELWYTRDDIARERDTSIGRVL
                     PDAALVEIAKAVPATTADLPRGHRAIARYGRHWVDAVRRAMEIPEDDLPPRTLPSDGP
                     PPPRVWAEKNPLAADRLGATRAALTAFAEERVIPVENVCSPDPLRRVVWQPPSEPDEA
                     AFTRALLDQGVRAWQAEIVAPMLVAAFAQFPG"
     gene            complement(4030..4614)
                     /locus_tag="PL014_09375"
     CDS             complement(4030..4614)
                     /locus_tag="PL014_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HOT57343.1"
                     /translation="MSSRSVSGRESPEFSRALKDLHRVRLRPEIRLTEVPAPQRIAPY
                     AVALTADVVDPRDTDDDLASGRFVLLHDPSSPEPWGGQWRAVTFARAELEPELATDPV
                     LGEVGWSWLIDALAIRGVEYTAEAGTVTRVVSQSFAGLADRPASVEMEVRASWTPLGP
                     EVGDHLLAWSDLLCTIAGLPPLPEGVVALPGPRR"
     gene            4739..5560
                     /locus_tag="PL014_09380"
     CDS             4739..5560
                     /locus_tag="PL014_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT57344.1"
                     /translation="MLALLALGSSVAWGTADFFGGLLSGRRPAIAVVGWSQGLGFLVI
                     SAVVLLRGDVSWQGWPLWALAAGLAGTTGLVSFYTALSTGTMGVVAPIAALGVIVPVA
                     LGVASGEQPSAWAWVGMVVAIVGVTLASGPEVSGAVSARPVVLAVVAAFCFGLALFFL
                     DRGARESTLMTLWGMRATSVTVLIVVALCLRSVGGVRRVELPALLAVGCGDLLANALF
                     AVASSRGEVSVAAVLGSLYPVVTAVLAWAVVKERLRPIQVLGSAMAVAGAVVIAL"
     gene            5584..6696
                     /gene="hemE"
                     /locus_tag="PL014_09385"
     CDS             5584..6696
                     /gene="hemE"
                     /locus_tag="PL014_09385"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOT57345.1"
                     /translation="MTADSRTADDRRPDSRTADQSSDSRPAADPKDSVLVRAARGQSV
                     PNAPVWFMRQAGRSLPEYREVRRGVGMLESCRRPDLVTEITLQPVRRHGVDAAIFFSD
                     IVVPLSAIGVDLDIVAGVGPVIAQPIRTRADLAQLRDLTPEDIPDIAESVRLLTAELG
                     GTPLIGFAGAPFTLASYLVEGGPSKNHEHTKALMHGDPQLWHDLCAQLAQISGTFLRV
                     QALAGASVVQLFDSWAGVLSKADYTASVMPHSAKALAAVADLDVPRIHFGVGTGELLP
                     LMGEAGADVVGVDYRVSLTDAIERLGGRYAVQGNLDPALLFAPWEPLERRVRTIVEEG
                     RAAPGHIVNLGHGVLPDTDPDVLTRVVELVHEVSAR"
     gene            6693..7208
                     /locus_tag="PL014_09390"
     CDS             6693..7208
                     /locus_tag="PL014_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT57346.1"
                     /translation="MSSTPHLRGRGVRLEPLSTAHAAPMEHVLADAGLYEFIGGSPPS
                     RADLEARYARQVAGPRGAVFAWHTWIIRLVDGDEPVGYVQATVNGDDSVAELAWVVGM
                     PWQGRGIASDAASAVVEFVDACGVERVIAHVHPGHVASQRVAARLGLVPTPRIVDGEV
                     EWEARSATRGR"
     gene            complement(7259..7564)
                     /locus_tag="PL014_09395"
     CDS             complement(7259..7564)
                     /locus_tag="PL014_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOT57347.1"
                     /translation="MGLLDLAAGHPTSTPHEQALAHAANGVAIYWRPGCPFTARLRLA
                     VRSRRDRARWVNIWEDDAGRAFVAGINHGNETVPTVVIDGIPHTNPDPSVVKAALSR"
BASE COUNT         1020 a         2692 c         2855 g         1141 t
ORIGIN      
        1 acctgctgct tgaccgccat ctcctcgaag accgcctcga tgacgaagtc ggcgtcggcg
       61 aagccgtcct tggaggtcga gccggtgacc aggcccgtga ggaaggacgc cttgtccggc
      121 gacacccgcc tcttgtcctt cagcgacgcg atctcggcgt gcacccagcc cacgcccttg
      181 gcgacgcggt cctcgtcgag atcggtcatg acgaccggca ccccgaggcg gcgcgcgaag
      241 agcagggcga gctggctggc catgagcccg gcgccgacga tgccgacctt ggtcaccggg
      301 cgggcgagcg ccttgtccgg tgcaccggcc gggcgcttgg cgcgcttctg cacgaggtcg
      361 aaggcgtaga gcccggcgcg caactcatca cccatgatga ggtcaccgag agcggcgtcc
      421 tccgcggcga aggcctcctc gcgagtcgcc gtccgcgcgg cgcgcacgag ctcgacggcc
      481 cgcatcgcac ctggcgcccg acccgcggtc ttggcgagga ctaccgagcg ggcgccatcg
      541 cacgcggcat cccacgcgct ctcgtcacgg tcgacgtcac gacgctgcac cggcacggca
      601 ccactgacga ccccggcggc ccaccgcagc gagtcctcga tgaagtccgc cggttcgagc
      661 agcacgtcgg cgatgccgag ggcggccacc tccttggcct tcagcatccg gttctgcgac
      721 aacgggttcg cgacgatgac ggtgagcgcg tcggccggcc cgatgaggtt gggcagcagg
      781 taacagccgc cccagcccgg aacgagcccg aggaaggtct cagggagggc gaacgcgggc
      841 acgccagcgg acaccgtgcg gtagtcgcag gccagggcga tctcgacgcc cccgcccatg
      901 gccgcgccgt tgatgaacgc gaacgtgggc accggcaggt ccatgatcga ggcgaacgcc
      961 gcgtgcccgg ctcgagcgat gctcacggcc tgctcgcggt cggtcacgta gggcacgccc
     1021 gacaggtcgg ccccgacggc gaagatgaac ggcttgccgg tgatgccgac agcctggatc
     1081 tcgccggctg ccgcacgggc cttcagcccg tcgacggtgg cctggagctc ggcgatcgac
     1141 gccgggccga aggtgttcgg ccgggtgtgg tccaggccgt tgtcgagcgt gatcagggcg
     1201 aggatgccgg cgccgccggg gagggcgacg tcctggaccg gggtgtgggt cacgacctcg
     1261 gtgggcagct gcggggtggg gggtgcggcg ggggtgtcaa caacagcgct cacgtcagtt
     1321 ctccttgccg tagtcggcgt ggtgcgggtt ctcccagatg acacagccac ccatgccgat
     1381 cccgatgcac atggccacca ggccgtagcg cacctgcggg ttctcctcga agtggtgggc
     1441 gagctgggtc atcaggcgaa cacccgacga cgccagcggg tggccgatgg cgatggctcc
     1501 accccagggg ttgacgcgct cgtcgtcgtc ggcgatgccg tagtggtcca ggaaggcgag
     1561 gacctgcacg gcgaacgcct cgttgagctc gaagagcccg atgtcctcga tggtcaggcc
     1621 agcctggcgc aacgccttct cggcagcggg gatcgggccg atgcccatga cctcgggctc
     1681 gacaccgacg aaggcgtagg agaccaggcg catgcgcacc ggcagcccca gttcggcggc
     1741 ggcggcctcg gaggcgagga tgcaggcggt cgcgccgtcg ttgaggccgg ccgcgttgcc
     1801 ggcggtgacg ttgccgtggg ggcggaacgg ggtcttcagg cccgcgagct cctccatggt
     1861 ggtgccggga cggggcggct cgtcgacggt tgccaggccc caacccttct cgacgtggcg
     1921 ggtggccacc ggcacgaggt cgggctgcac cttcccggcg gcgtaggccg cggcgagctt
     1981 gcgctggctg ttgacggcgt acgcgtcgca ccgctccttg gtgaggtgcg ggaagcggtc
     2041 gtgcaggttc tcggcggtct tgcccatgac cagggccgac tcgtcgacga tccgctcgcc
     2101 gatgatccgc gggttcgggt cgatgccctc acccatgggg tggcggccca tgtgctcgac
     2161 cccaccggcg atggcgacgt cgacggcccc gaaaccgatc gaggaggcca cgttcgtgac
     2221 ggcggtcatc gcaccggcgc acatgcggtc gaccgagtag cccggggtgg tcttgggcag
     2281 gcccgcgagc aggccggcca ggcgacccag cgtcaacccc tggtcgccga tctgcgtggt
     2341 ggcggcgatc gcgacctctt cgacccgttc cttgggcagt tcggggtggc gtcgcagcag
     2401 ttcacgcatg cacttgatga cgaggtcgtc ggcgcgggtc tgggcgtaga tgcccttctc
     2461 gccggctttg ccgaacgggg tgcggacgcc gtcgacgaag acgacttcct ggagggtacg
     2521 gggcacggcg gtggcctcct gggtcacggc ggacatggtg cacccggatg ccgggcgcgg
     2581 ccccgtcagc gtactaagcg agcgcttagt acgggagggc tggcggggtg agagacgtca
     2641 cgcggaggtg acttctcggc gatcccggga tcgccggcgt ccctgaggag catcggcatc
     2701 cggcgtggcg ggtcagcccg ggaactgggc gaacgcggcc acgagcatcg gggcgacgat
     2761 ctcggcctgc caggcccgca ccccctggtc gagaagggcc cgggtgaagg cggcctcgtc
     2821 cggttcggac gggggctgcc agaccacgcg ccgcaacggg tcgggggagc agacgttctc
     2881 gacggggatg acccgctcct ccgcgaacgc ggtgagcgct gcccgggtgg caccgaggcg
     2941 atcggccgcc aacgggttct tctccgccca cacgcgcggc ggtggcgggc cgtcagaggg
     3001 cagggtgcgc ggcggcaggt cgtcctcagg gatctccatg gctcgtcgca ccgcatcgac
     3061 ccagtgccgg ccgtagcggg cgatggcccg gtgcccgcgc ggcaggtcag cggtggtggc
     3121 gggcacggcc ttggcgatct cgacgagcgc ggcatcgggg agcacccgcc cgatcgaggt
     3181 gtcccgctcc ctggcgatgt cgtcgcgggt gtaccacagc tcgcggacga tggccacgga
     3241 ccgccgtgaa cgcagcgtgt tgatccccga cgttcgccgc cacgggtcga cccgctcggt
     3301 gggggcccac tgcagcagcg cggcgaactc ctggcgtgcc cactcggcct tgccctgcgc
     3361 ggcgaggtcg acccccatga ggttgcgtag ttcgtcgagc acctcgacgt cgagggcggc
     3421 gtagcgcaac cacggctcgg gcagcggccg ggtcgaccag tcgacggcgg agtgctcctt
     3481 ggccaggccc agcccgaggt agtgctcgac cacggcagcc agcccgacgc gaggcagccc
     3541 gaggatccgc ccggccagct cggtgtcgaa gagctgcacc ggtcgcagcc cgacctcggc
     3601 caggcaggca aggtcctggg tggcggcgtg caggatccac tcggcagcac cgatcgcctc
     3661 acccagggga ctgaggtcag ggcagcccat cggatcgatc agccaggtgc ccgcaccctc
     3721 tcgacgcacc tgaacgaggt aggcgcgctg cccgtagcgg tagccggacg cgcgttcggc
     3781 gtcgatgccg accggcccct caccggcagc gatggccttc gcggcccggg cgagctcgcg
     3841 ctcggtctcg acgacgtggg gcacaccacc ggacggctcg atgagcggca ccagggtgac
     3901 gggcgcggcc tcggggacgc ggtggtcgct ggaacttcgg tcgtgctggt cccctggcgg
     3961 gggtggcgtc gtggtctcgg tgatcatcgg tccgtggtgt ccgtcagtgg tgtctgtcgc
     4021 tggtgtccgt cagcggcggg gtccggggag ggcgacgacg ccctcgggaa ggggcggcag
     4081 acccgcgatg gtgcacagca ggtcggacca tgccagcagg tggtctccga cctccgggcc
     4141 gagtggggtc caggacgcgc gcacctccat ctcgacactc gcggggcggt cggccaggcc
     4201 ggcgaagctc tgcgagacga cccgtgtcac cgtgcccgcc tcggccgtgt actcgacgcc
     4261 acggatcgcg agcgcgtcga tgagccagct ccaccccacc tcgccgagca ccgggtcagt
     4321 cgccagctcg ggttcgagct cggcccgggc aaaggtcact gcacgccact gcccgcccca
     4381 cggctcgggg gagctggggt cgtgcagcag cacgaaccgg ccggaggcga ggtcgtcgtc
     4441 ggtgtcacga gggtcgacga catcagcggt gagcgcgacg gcatacggcg cgatgcgctg
     4501 gggcgccggg acctcggtga gtcggatctc ggggcgcaag cgcacccggt ggagatcctt
     4561 gagggcgcgg gagaactccg gggactcccg accggagacg ctgcggctgg acacgcctgc
     4621 aatgtatgcc gatccggccc cggcgcccgt caggcacgcc gcgaggagac gggcgaccac
     4681 gagcacgggc agggctatcg cgagcactga ccagggcgcg cgcggctaca ttcgagcggt
     4741 gctcgccctc ctggccctcg ggtcctccgt cgcgtggggg accgctgact tcttcggcgg
     4801 gctcctcagc ggacgccgcc cggccatcgc ggtggtcggc tggtcgcagg ggctggggtt
     4861 cctcgtcatc agcgccgtcg tgctcctgcg tggcgacgtg tcctggcagg gctggcccct
     4921 ctgggcgctc gccgccggtc ttgccgggac gaccggactc gtcagcttct acacggccct
     4981 gtccaccggg accatgggag tcgtcgcgcc gatcgccgca ctgggcgtga tcgtcccggt
     5041 tgccctgggc gtcgcgtccg gtgagcagcc atccgcctgg gcctgggtcg gaatggtggt
     5101 ggccatcgtc ggcgtcaccc tggcctcggg gccggaggtc agcggagcgg tgtccgccag
     5161 gcccgtggtg ctggctgtgg tcgcggcctt ctgcttcggc ctggccctgt tcttcctcga
     5221 ccggggagcg cgcgagtcga ccctcatgac gttgtggggg atgcgggcca cgtcggtgac
     5281 cgtgctgatc gtcgtggcgc tctgcctgcg cagcgtcggc ggggtgcggc gcgtggagct
     5341 gccggcgttg ctggccgtcg gctgcggtga cctgctggcc aacgccctgt tcgccgtggc
     5401 gtcctcgcgc ggtgaggtca gcgtcgccgc cgtgctcggc tcgctctacc ccgtcgtcac
     5461 cgcggtgctc gcgtgggccg tggtcaagga acggctgcga cccatccagg tgctcgggtc
     5521 cgcgatggcc gtggcgggtg ccgtcgtcat cgcgctctga ccagggtggg agaatcactg
     5581 atcgtgactg ccgactcccg caccgccgac gaccgacgtc ccgattcccg caccgctgac
     5641 cagagcagcg acagccgccc cgccgccgac cccaaggact cggttctcgt tcgggccgcg
     5701 cgggggcagt ccgtcccgaa cgcgccggtg tggttcatgc gacaggccgg gcgctcgctc
     5761 cccgagtacc gcgaggtgcg gcgcggcgtc ggcatgctcg agtcgtgccg gcggcccgac
     5821 ctcgtcaccg agatcaccct ccagccggtg cgccgccacg gcgtggatgc cgcgatcttc
     5881 ttctccgaca tcgtcgtccc cctctccgcg atcggtgtcg acctcgacat cgtcgccggg
     5941 gtcgggccgg tcatcgccca gccgatccgc acgcgggccg acctcgccca gctgcgcgac
     6001 ctcacccccg aggacatccc ggacatcgcc gagtccgtgc gcctgttgac cgctgagctc
     6061 ggtggcaccc ccctcatcgg cttcgccggg gcgccgttca ccctcgcgtc ctacctcgtc
     6121 gagggcggcc cgtcgaagaa ccacgagcac accaaggcgc tcatgcacgg tgacccgcag
     6181 ctctggcacg acctgtgtgc ccagctcgcg cagatctccg gcacgttcct tcgcgtgcag
     6241 gcgctggccg gagcctcggt cgtgcagctg ttcgactcgt gggccggggt cctcagcaaa
     6301 gccgactaca cggcatccgt catgccgcac tcggcgaagg ccctcgccgc ggtggccgac
     6361 ctcgacgtgc cgcgcatcca cttcggtgtc ggcaccggtg agctgctgcc gctcatgggc
     6421 gaggccggcg ccgacgtcgt cggtgtcgac taccgcgtct cgctcaccga cgccatcgag
     6481 cggctcggcg ggcgctacgc ggtgcagggc aacctcgacc cggccctgct cttcgccccg
     6541 tgggagcccc tcgagcgacg cgtgcgcacg atcgtcgagg aggggcgcgc ggcacccggg
     6601 cacatcgtca acctcggcca cggcgtgctg cccgacaccg accccgacgt gctcacccgc
     6661 gtcgtcgagc tcgtgcacga ggtctccgcg cggtgagcag caccccgcac ctgcggggcc
     6721 gcggtgtgcg tctggagccg ctctcgaccg cccacgccgc accgatggag cacgttctcg
     6781 cggatgccgg cctgtacgag ttcatcggcg gctcgccgcc gtcgcgggct gacctcgagg
     6841 cgcggtacgc ccgtcaggtg gccggtccgc gcggtgccgt gttcgcctgg cacacgtgga
     6901 tcatccggct ggtcgacggc gacgaacccg tcggctacgt gcaggccacc gtcaacggtg
     6961 acgactcggt cgccgagctc gcctgggtcg tcgggatgcc gtggcagggc cgggggatcg
     7021 cctcggatgc cgcgtcggcc gtcgtggagt tcgtcgacgc ctgcggggtc gagcgggtga
     7081 tcgcgcacgt gcaccctggc cacgtggcgt cgcagcgggt cgcggcccgc ctgggactgg
     7141 tgccgacgcc gcgcatcgtc gacggtgagg tcgagtggga ggcgcgctcc gcaaccaggg
     7201 gtcgttgacc ggatcgggcg gtcgcaccca tggtgatgcg gttcttgccg cgcgtgggtc
     7261 agcgcgacag ggcggccttc acgaccgagg ggtcggggtt ggtgtggggg atgccgtcga
     7321 tcaccaccgt gggcaccgtc tcgttcccgt ggttgatgcc tgcgacgaag gcacgacccg
     7381 cgtcgtcctc ccagatgttg acccagcggg cccggtcgcg ccgggaacgg accgccaggc
     7441 gcaggcgcgc cgtgaacgga cagcccggtc gccagtagat cgcaacaccg ttcgcggcgt
     7501 gagcgagcgc ctgctcgtgt ggggtcgagg tcgggtgccc ggcggcgaga tccagaagtc
     7561 ccatgcagac agggtacgaa cgggtggtga ccgtgggcga tcactggtgg tgagttccca
     7621 ccccaccctc gcgatggcgc gcgacgggca cctgtcctgc acgctcgccg ccgctgagcg
     7681 ggacaatctt cccggacatc acacctag
//
LOCUS       DAOSOC010000121         7563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9716_length_7563_cov_6.761854, whole genome shotgun sequence.
ACCESSION   DAOSOC010000121 DAOSOC010000000
VERSION     DAOSOC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9716_length_7563_cov_6.761854"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PL014_09400"
     CDS             complement(<1..932)
                     /locus_tag="PL014_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57348.1"
                     /translation="MSPSQQAQPDRPEVEVTVDDRALEPLVEADVIEVDVHEEVGKHG
                     RCTLLVQNWDADVRAVRHSDKGPFVPGAAIGVSLGYHAALKPVFEGVVTALTAHFSGG
                     RPVLRVEARSRSILLAHPPRSRVLADVSDADVASAVAADYDLTTDAEVGVTREAVVSD
                     RVSDWDFLLARAQRLGWVLYVRGTTLVMRPPAQTENPPEFEYTRSLVELHLTDDLTHA
                     IDTATGVGWDPAALETVESEQSSSAAAIDTGDRSDHAAAVGKTSWPLRSARDESAAET
                     AADAADARAVGRQRDAALAHLHGRGVVLGDPTLR"
     gene            complement(949..1635)
                     /locus_tag="PL014_09405"
     CDS             complement(949..1635)
                     /locus_tag="PL014_09405"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT57349.1"
                     /translation="MSATATALTKLTIDAYTDLEFRTPAGISWTALLNPTELAFSRKN
                     RYEATQSAGTSAPQQSFSGGEPDQVSIDLLLDGTGVVGDPGSIGPTLDELVGLTTFQG
                     DTHQPYYVHAHWGRFSFRGILTQVDVTYKLFDRAGEPLRATVKLALKEALSPQEVQAQ
                     DRPESPDLFQTWLVRDGERLDAIAHRVYGDARYWRPLAAANHLTNPRFLRPGTVLVLP
                     PVAARTGGRR"
     gene            complement(1632..1808)
                     /locus_tag="PL014_09410"
     CDS             complement(1632..1808)
                     /locus_tag="PL014_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57350.1"
                     /translation="MAVVIGEVVTETVVAQTAAASETASAPASAIVDDAQVELVVRRA
                     TERVLETLRREWDR"
     gene            complement(1808..2272)
                     /locus_tag="PL014_09415"
     CDS             complement(1808..2272)
                     /locus_tag="PL014_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOT57351.1"
                     /translation="MLPDTSPAVLPFTTARFVLAIDGLRSVSFSKCAGLAGEVSVEEY
                     QEGGENRFAHRFPSRASFPNLVLTQGVGPTRELWDWFAEFHVTGLVAPRDGTVSLLST
                     VDGALVPVRVWSFTRGWPAKLTGPDLDAQASAVGVESLEIAHHGLELRSVVA"
     gene            complement(2277..2705)
                     /locus_tag="PL014_09420"
     CDS             complement(2277..2705)
                     /locus_tag="PL014_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOT57352.1"
                     /translation="MTALHFAVSWGDSQNTSSFSEVSGLTVEAEVVEYRGGADPQLST
                     RKMPGLKKFSNVTLKRGIAPAEAGNGLFDWYNSIAAGTVARRSVTVSLLNEERNPVMT
                     WKIKEAWPVKIEGPSLKSTGTDVAIESVEFACEGIEVEVA"
     gene            complement(2731..5328)
                     /locus_tag="PL014_09425"
     CDS             complement(2731..5328)
                     /locus_tag="PL014_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HOT57353.1"
                     /translation="MSYQSPGVYIEEVPSGPQPIAAASTSVVAVLGTTRKGPVLSPTR
                     VTSWSDYVRVFGPATSRGVCAESVFGFFENGGPAAWIVRVDPSTAAEWTVRDSQGTPA
                     TSFLATAASPGSWANTVSLRISPDGSGGLGTMARATVSGPVTAAVGAATVPVVASGGL
                     AVGDAVLLTSRTATSAATVTAVSATSIGVNNTGASITLAAGDGVALTTVAAPSTLVFA
                     AGSGVRAHDLLVIQRPDGSQVSALVAAVDQDGPTMTVTLAAPLGAAVTAAGFARRRAT
                     FTGRTTGNTGGTLPLSRIQWDVADAGPRNADSQLDNARVTLPGGGSATWTGTTFAVAG
                     GGDVPPGPITAEAQVRVATYTASTSFAPADVAEVLARYSFLPVGTTLSLSNGTATTTV
                     ARTGAGVTNTDGTRGGDAFGPTYTSATFVLPGDASLGVVVRCPVAPEVGDFVAFSGTK
                     TFRITTVSPVDGDVYVLDFAETTTLAGETGGDGGRFALIGVAPTTAFPARFTLSVDDD
                     GATVETFAGLALHPSHPRYFAKDGVVNGISGHVRLAPRAAGAPAISLATMPSGATRTQ
                     DGVDQPATNQHYVSALEKLETVDEPAMVIAPDSVKVQDPLLQADLIGKVVRHCEQFRR
                     FAIIDGPDLSSIADYQRDKALVDWRNATLSSTYAAAYAPFLKIVTIDPDAPDRFTMVP
                     PSGFVAGVFARTDRERGVHKAPGNERVNGIVGLAEQYSQRRQDLLNPNAVNLIRSFPG
                     RGTRIWGARNATDDVTWRYVNVRRLFSMIETSVDRSTQWVVFEPNTATTWIRIKVSVE
                     NFLDQQWRAGALAGTRPEEAYRVRVGLGETMTETDIDVGLVITEVAIAPAKPAEFVVF
                     RFSHKRLSE"
     gene            complement(5346..5945)
                     /locus_tag="PL014_09430"
     CDS             complement(5346..5945)
                     /locus_tag="PL014_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57354.1"
                     /translation="MTITLGTTTTELRHRIRLVHATTGQPIAPLAVAPAPLALGWVAR
                     VVGPDVIVTATTSDAGSAARPAPTTLDVVVLDGALADLLALPAPAADQPPNSIGVALG
                     APEIEVPVTPAPMTLTVVLTGQGGTAPSTGRTVAVRGTSGTTIALPETATPGTYRSTA
                     RTWTADHHPGDLRVDGQSVRKVAVDVTRADTRIHVVDPT"
     gene            complement(5942..6469)
                     /locus_tag="PL014_09435"
     CDS             complement(5942..6469)
                     /locus_tag="PL014_09435"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HOT57355.1"
                     /translation="MSEALVDVIHQNTPDLGDWVVLHSLNSADTGPDTTKAALALLAV
                     APHPHLVNRPLVERAAGLVRAPLALRLHYLVTVFGAHDEAQTKIARIVQVFHTTPIIG
                     RSMLQPPLSTAVDSITVRLLSPSAEERNQIWSTLGRPGRLALFYEVDVAPVEVIEREG
                     AGRVRTHRVGYEVLA"
     gene            complement(6478..6690)
                     /locus_tag="PL014_09440"
     CDS             complement(6478..6690)
                     /locus_tag="PL014_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57356.1"
                     /translation="MAVSAWRRRFLLDVWAEPRAVDGLPRILRARVRDMTTDDTTFVG
                     SIAEIEAVLDRSLDDGGITHRRWERS"
     gene            complement(6977..>7563)
                     /locus_tag="PL014_09445"
     CDS             complement(6977..>7563)
                     /locus_tag="PL014_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57357.1"
                     /translation="HPGAGLGVATTGIHRVVRLPIALCQMVAGSGTIAAVCSMDDALH
                     DERVTTEQLRAAADLLPEERRDAVIRAIELTDATCESVGETRTRFLLRDLGFSVVSQH
                     PITNAQGFVGRVDFLVEDLVVVEFDGLVKYGGQDGRAALAAEKARESRIVDCGYEVVR
                     LVWSDLGQPADVARRIREARRRALNRRSATASAR"
BASE COUNT         1021 a         2541 c         2895 g         1106 t
ORIGIN      
        1 cagcgcaggg tcgggtcacc gaggacgacc ccgcggccgt gcaggtgggc cagggcggcg
       61 tcgcgctggc ggccgacagc tcgggcgtcg gccgcgtcgg cggcggtctc ggcggcgctc
      121 tcgtcgcggg ccgagcgcaa cggccacgac gtcttcccga cggcggcggc gtggtcgctg
      181 cggtcaccgg tgtcgatcgc ggccgcgctg gacgactgct cggactccac cgtctccagg
      241 gcagccgggt cccacccgac gccggtcgcc gtgtcgatgg cgtgggtcag gtcgtcggtg
      301 aggtgcagct cgacgaggga gcgggtgtac tcgaactcgg gcgggttctc ggtctgtgcc
      361 ggggggcgca tgaccagggt ggtgccgcgc acgtagagca cccagccgag gcgctgagcc
      421 cgcgcgagta ggaagtccca gtcgctgacc cggtcggaga cgaccgcctc gcgggtgact
      481 ccgacctcgg cgtcggtggt gaggtcgtag tcggcggcga cggccgaggc gacgtccgcg
      541 tcggacacgt cggcgagcac gcgcgagcgg ggcgggtggg ccaacaggat cgagcggctg
      601 cgcgcctcga cccgcagcac cgggcgaccc cccgagaagt gggcggtcag ggccgtgacg
      661 acgccctcga acaccggctt gagcgcggcg tggtagccca gcgacacccc gatcgcggca
      721 ccggggacga acgggccctt gtcgctgtgg cggaccgcgc gaacgtcggc gtcccagttc
      781 tggacgagca gggtgcagcg cccgtgcttg ccgacctcct cgtggacgtc gacctcgatg
      841 acgtcggcct cgacgagcgg ctcgagggcc ctgtcgtcga cggtgacctc gacctcgggc
      901 cgatccggct gggcctgctg gctggggctc atcgcggccc tcccccggtc atcgccggcc
      961 cccggtccgg gctgcgaccg gcggcagcac gagcacggtg ccgggccgca ggaagcgcgg
     1021 gttggtcagg tggttggccg ccgcgagcgg gcgccagtac cgtgcgtcgc cgtagacccg
     1081 gtgggcgatg gcgtcgagcc gctcgccgtc ccgcacgagc caggtctgga acaggtcggg
     1141 tgactcgggt cggtcctgcg cctgcacctc ctgcggcgag agcgcctcct tcagggcgag
     1201 cttcacggtg gcccgcagcg gctcccccgc acggtcgaac agcttgtagg tgacgtcgac
     1261 ctgggtgagg atcccgcgga agctgaaccg gccccagtgc gcgtgcacgt agtacggctg
     1321 gtgggtgtcg ccctggaagg tcgtcagccc gacgagctcg tcgagggtcg ggccgatgct
     1381 gccggggtca ccgacgacgc ccgtgccgtc gagcagcagg tcgatcgaga cctggtcggg
     1441 ctcaccgccc gagaaggact gctggggggc tgacgtcccg gcggactggg tcgcctcgta
     1501 gcggttcttg cgactgaagg cgagctcggt ggggttgagc agggcggtcc agctgatgcc
     1561 ggccggcgtc cggaactcca ggtcggtgta cgcgtcgatg gtcagcttcg tcagggccgt
     1621 ggcggtggcg ctcatcggtc ccactcccgt cgcagcgtct ccaggacccg ctcggtggcc
     1681 cgccgcacga cgagctcgac ctgcgcgtcg tcgacgatgg cggatgccgg tgcgctcgcg
     1741 gtctcgctgg cggctgcggt ctgcgccacc accgtctcgg tgaccacctc gccgatgacg
     1801 acggccatca cgccaccacc gaccgcagct cgagcccgtg gtgggcgatc tcgagcgact
     1861 cgacgccgac cgcgctggcc tgcgcgtcga gatccgggcc ggtcagcttg gccggccagc
     1921 cgcgggtgaa ggaccacacc cgcaccggca cgagggcgcc gtcgaccgtc gagagcaggc
     1981 tcaccgtgcc gtcgcggggg gccacgagac cggtgacgtg gaactcggcg aaccagtccc
     2041 agagctcgcg ggtgggcccg acaccctggg tgagcacgag gttcgggaac gaggcccgac
     2101 tcgggaagcg gtgcgcgaac cggttctcgc caccctcctg gtactcctcg accgacacct
     2161 cgcccgcgag ccccgcgcac ttgctgaagc tcaccgagcg cagaccgtcg atggccagca
     2221 cgaagcgagc cgtcgtgaac ggcaggaccg cgggagaggt gtcgggaagc atcggctcag
     2281 gccacctcga cctcgatgcc ctcacaggcg aactcgaccg actcgatggc gacgtcggtg
     2341 ccggtggact tcaggctggg gccctcgatc ttgaccggcc aggcctcctt gatcttccac
     2401 gtcatcaccg ggttgcgctc ctcgttgagc aggctcacgg tgaccgagcg ccgtgccacg
     2461 gtgccggcgg cgatggagtt gtaccagtcg aagagcccgt tgcccgcctc ggcgggggcg
     2521 atgccccgct tgagggtgac gttggagaac ttcttcagcc ccggcatctt gcgggtgctg
     2581 agctgggggt cggccccacc gcggtactcg acgacctcgg cctcgaccgt caggccgctg
     2641 acctcggaga agctcgaggt gttctgggag tcgccccagc tcacggcgaa gtgcagtgct
     2701 gtcatgggat acacgtcatg ctccttctgg tcactcggac aggcgcttgt ggctgaagcg
     2761 gaagaccacg aactcggccg gcttggccgg ggcgatggcc acttcggtga tgaccaggcc
     2821 gacgtcgatg tcggtctcgg tcatcgtctc cccgagcccg acccgcaccc gataggcctc
     2881 ctcgggccgg gtaccggcga gcgctccggc ccgccactgc tggtccagga agttctcgac
     2941 ggagaccttg atgcggatcc acgtcgtcgc cgtgttcggc tcgaagacca cccactgcgt
     3001 cgagcggtcg acgctcgtct cgatcatcga gaacagccgg cgcacgttga cgtagcgcca
     3061 ggtgacgtcg tcggtggcgt tgcgcgcccc ccagatccgc gtgccgcggc cggggaagga
     3121 acggatgagg ttgacggcgt tcgggttgag caggtcctgg cgacgctggc tgtactgctc
     3181 ggccaacccg acgatgccgt tcacgcgctc gttgccgggt gccttgtgga ccccgcgctc
     3241 gcggtcggtg cgggcgaaga ccccggcgac gaacccggag ggcggcacca tggtgaaccg
     3301 gtccggagcg tcggggtcga tcgtcacgat cttcaggaac ggcgcgtagg ccgccgcgta
     3361 ggtggagctg agggtggcgt tgcgccagtc gacgagcgcc ttgtcccgct ggtagtcggc
     3421 gatgctcgac aggtcggggc cgtcgatgat ggcgaagcgc cggaactgct cgcagtggcg
     3481 cacgaccttg ccgatgagat ccgcctgcag cagcgggtcc tggaccttca ccgagtccgg
     3541 ggcgatgacc atggccggct cgtcgacagt ctcgagcttc tcgagcgcac tgacgtagtg
     3601 ctggttcgtc gccggctggt cgacgccgtc ctgggtgcgg gtggcacccg acggcatggt
     3661 cgcgaggctg atggccggtg ccccggccgc ccggggggcg agccggacgt gaccggagat
     3721 gccgttcacg accccgtcct tggcgaagta ccgcgggtgc gaggggtgca gggccagacc
     3781 ggcgaaggtc tcgacggttg ccccgtcgtc gtccacggag agtgtgaagc gcgccgggaa
     3841 ggcggtcgtc ggggcgaccc cgatgagggc gaaccggcca ccgtcaccgc cggtctcccc
     3901 ggccagggtc gtggtctcgg cgaagtcgag cacgtacacg tcgccgtcga ccggggacac
     3961 cgtggtgatc cggaaggtct tcgtgccgga gaaggcgacg aagtcaccga cctcgggggc
     4021 gaccgggcag cgaacgacga ccccgagcga cgcgtcaccg ggcagcacga acgtcgcgct
     4081 cgtgtacgtc ggcccgaagg cgtcaccgcc gcgggtgccg tcggtgttgg tcaccccggc
     4141 gccggtgcgg gcgacggttg tcgtcgccgt gccgttgctc aggctgaggg tcgtgccgac
     4201 cggcaggaag gagtagcgcg cgagcacctc ggccacgtcg gccggggcga acgacgtgga
     4261 tgccgtgtac gtcgccaccc ggacctgcgc ctccgcggtg atcgggcccg ggggcacgtc
     4321 accgcctccg gccacggcga aggtcgtgcc ggtccacgtc gccgagccgc caccggggag
     4381 agtgacccgg gcgttgtcga gctggctgtc ggcgttgcgc gggccggcgt cggcgacgtc
     4441 ccactggatc ctgctcagcg gcagggtccc tccggtgttg ccggtggtgc gtccggtgaa
     4501 ggtcgcgcga cgtcgggcga agcctgcggc ggtgaccgcg gcaccgagcg gggcggccag
     4561 agtcaccgtc atcgtcgggc cgtcctggtc gaccgcggcg acgagggcgc tgacctgcga
     4621 cccgtccggt cgctggatga ccaacaggtc gtgggcacga accccggacc ccgcggcgaa
     4681 gacgagggtg gagggggcgg ccacggtggt gagggcgacg ccatcccccg cggcgagggt
     4741 gatgctcgcc ccggtgttgt tcacaccgat cgacgtcgcg gacacggcgg tgacggtcgc
     4801 ggcgctcgtg gcggtgcggg aggtgaggag cacggcatcc ccgaccgcga gcccaccgct
     4861 cgccaccacc gggacggtgg cggcgccgac cgctgcagtg accgggccgg acacggtcgc
     4921 gcgggccatc gtcccgagac cgcccgagcc gtcgggcgag atgcgcaggg acacggtgtt
     4981 ggcccacgaa ccggggctgg ccgccgtggc gaggaagctc gtcgctgggg ttccctggct
     5041 gtcgcgcacg gtccactcgg cggcggtcga cgggtcgacg cggacgatcc acgccgcggg
     5101 gccgccgttc tcgaagaagc cgaacaccga ctcggcgcac actcccctcg aggtggccgg
     5161 gccgaacacg cggacgtagt ccgaccagct cgtgacccgg gtcggactga gaacggggcc
     5221 cttgcgggtc gtgccgagca cggcgacgac actggtcgat gcggcggcga tcggttgcgg
     5281 ccccgacggg acctcctcga tgtacacccc gggtgactgg tagctcacgg ttgcctcctt
     5341 ggcgatcagg tcgggtcgac cacgtggatt cgggtgtcgg cgcgcgtgac gtccacggcg
     5401 accttgcgca cgctctgtcc gtcgacccgc aggtcgccgg ggtggtggtc ggcggtccag
     5461 gtgcgggccg tcgagcggta ggtgccaggg gtggcggtct ccggcagggc gatggtggtt
     5521 cctgaggtgc cgcggacggc gacggtgcgg ccggtgctgg gcgcggtgcc gccctggccg
     5581 gtgaggacga cggtgagggt catcggcgcc ggggtgacgg gcacctcgat ctcgggtgcg
     5641 ccgagggcga cgccgatcga gttcggcggc tggtccgcag ctggtgccgg cagggccagg
     5701 aggtcggcga gtgccccgtc gaggacgacc acgtcgaggg tggtgggcgc agggcgcgcc
     5761 gcgctcccgg catccgaggt cgtcgcggtg acgatgacgt ccgggccgac cacccgcgcg
     5821 acccacccca gggccagcgg tgcgggggcc acggcgaggg gggcgatcgg ttggccggtg
     5881 gtggcgtgga cgagccggat gcggtggcgc agctcggtgg tcgtggttcc cagggtgatg
     5941 gtcatgcgag cacctcgtag ccgacccggt gggtgcgcac ccgaccggcg ccctcgcgct
     6001 cgatgacctc gaccggggcg acgtcgacct cgtagaacag ggccaggcgt ccggggcggc
     6061 cgagggtcga ccagatctgg ttgcgttcct ccgcgctcgg gctgagcagt cgcacggtga
     6121 tgctgtcgac cgccgtggac agcgggggtt ggagcatcga gcggccgatg atcggggtcg
     6181 tgtggaagac ctgcacgatg cgggcgatct tggtctgggc ttcgtcgtgg gcgccgaaca
     6241 ccgtcacgag gtagtgcagg cgcagcgcca ggggtgcgcg cacgaggccc gcggcccgtt
     6301 cgacgagggg acggttcacc aggtgcgggt gcggcgcgac ggcgagcagg gccagggcgg
     6361 ccttggtggt gtcggggccg gtgtcggccg agttgagcga gtgcagcacc acccagtcgc
     6421 cgaggtcggg tgtgttctgg tgaatgacgt cgaccagggc ctcgctcacc gcgcggatca
     6481 tgaccgctcc cagcggcggt gcgtgatgcc gccgtcgtcg aggctgcggt cgagcaccgc
     6541 ctcgatctcg gcgatcgagc cgacgaaggt cgtgtcgtcg gtggtcatgt cacgaacccg
     6601 ggcccgcagg atgcgcggca gcccgtcgac cgctcgcggc tcggcccaga cgtcgagcag
     6661 gaacctgcgt cgccatgcgc tcaccgccat ccgcgtctcc ctctgtgtgg cggcccacgt
     6721 ttccgcggtg gtgtcacggt ggcgtcgtgg cgtcagggtg ggtcagtcac gggggcgtca
     6781 cggtcgtctc ggcactgtcg aacgcagaag ttcggacccg acgcaccact tcggatcgca
     6841 cgcagcagtt caaatcgcac gcagctatta ccggacacca tccgaacttc cgcgtccccg
     6901 tcgaggttct gcgcggcatc cgaactcccg cgtcagcgag cggatgccgt cgcgctggtg
     6961 ggtggatgcc gtcgcgtcag cgagcggatg ccgtcgcgct gcggcggttg agcgcccgtc
     7021 ggcgcgcctc gcggatgcgt cgggccacgt ccgcgggctg gccgaggtct gaccagacga
     7081 gacggaccac ctcgtaaccg cagtcgacga tgcgcgactc gcgagccttc tcggcagcca
     7141 gtgcggcccg gccgtcctga ccgccgtact tgaccagccc gtcgaactcg acgacgacca
     7201 ggtcctcgac gaggaagtcg acccggccca cgaagccctg tgcgttggtg atggggtgct
     7261 ggctgaccac gctgaagccg aggtcacgca ggaggaagcg ggtcctggtc tcgccgaccg
     7321 attcacaggt cgcgtcggtg agctcgatcg ctcggatgac ggcgtcgcgg cgctcctcgg
     7381 gcagcaggtc ggcggctgcc cggagctgtt ccgtggtgac ccgctcgtcg tgcagggcgt
     7441 cgtccatcga gcacacggct gcgatcgtcc cggagccggc caccatctgg cacagcgcaa
     7501 tcggcagacg cacgaccctg tggatgccgg tggtcgccac ccccagcccc gcgccggggt
     7561 ggg
//
LOCUS       DAOSOC010000122         7548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9754_length_7548_cov_9.487789, whole genome shotgun sequence.
ACCESSION   DAOSOC010000122 DAOSOC010000000
VERSION     DAOSOC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9754_length_7548_cov_9.487789"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PL014_09450"
     CDS             complement(<1..1027)
                     /locus_tag="PL014_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT57358.1"
                     /translation="MVEDGTWVGAGTPPPGVSSRARARAALALVGRNRTPGNPVLEPL
                     LQVVRGTHPKADVAVIERAYAVAERAHEGQKRRSGDDYITHPLAVTTILAELGMTPTT
                     LAAALLHDTVEDTSYSLEALRRDFGDEIAMLVDGVTKLDKVTYGQAAQAETVRKMVVA
                     MARDIRVLVIKLADRLHNARTWRYVSQESAARKAKETLEIYAPLAHRLGMNTIKWELE
                     DLSFQALYPKVYDEIVRLVAERAPAREEYLATVRDEVSADLATAKIKATVTGRPKHYY
                     SVYQKMIVGGRDFEQIYDLVAVRVLVETVRDCYAALGALHARWNPVPGRFKDYIAMPK
                     FNMYQSLH"
     gene            complement(1079..2272)
                     /gene="secF"
                     /locus_tag="PL014_09455"
     CDS             complement(1079..2272)
                     /gene="secF"
                     /locus_tag="PL014_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOT57359.1"
                     /translation="MSSRFATLGNDLYTGEKSVPVVAQRRRFYIASAVVMLIAVLGLV
                     FQGLNLGLEFRGGSEFRVATTSLPATYEDIARQAIGATESQRAVNVTVVGNNTVRVQT
                     ERLNDTDSQAVRQNLAEAFGVPTSEVSGTFIGPSWGASVSQQALRALVIFLALVAVVM
                     SIYFRNWKMAAAALIAVVHDMIITVGIYALIGFEVSPATMIGFLTVLGYSLYDTVVVF
                     DKVRENTTEAFANGRMSYAEAANLAVNQTMVRSINTTIIGVLPVAAVLAVGAIWLGPG
                     VLLDLSLVLFVGIIVGAYSSIFIATPLLVSLRRNEPAVIELAKRASRYQARRAKDVAA
                     AETAEAFGEGDGGAEDPRVAVSAGSGTRAAASGDASTLSGRAVHKYAQSGPRNQPRRP
                     PKSKR"
     gene            complement(2269..4065)
                     /gene="secD"
                     /locus_tag="PL014_09460"
     CDS             complement(2269..4065)
                     /gene="secD"
                     /locus_tag="PL014_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOT57360.1"
                     /translation="MSLKTQRSAARRALLALTILSLALMGLLAGAMHWSDAQATPKLG
                     LDLKGGTQIILEPKTVEGQTVTAEQLNQARNIIVQRVDAGGVSGAEVTTQGDRNIVVS
                     IPEIPTQEVRDAISKSSQLQFRPVLAIAAGSPQPQPTVSPSGTASPDPSASPAASGAP
                     AASPSPTPTVASSASPTNNSSVPEALMAPTPSPSATASPSPTPSPAASVASPTSPTDL
                     AWITPELSTQFELLNCADPAALDNLIDDPAKPLVTCQDDDSVKYILGPVAVAGDKISD
                     ATSGYRTNQQGVVTNDVEIALTLTSEGAKQYADISRAMVALPEPQNQLAATLDSRVIV
                     APRFNEAILDGRASITGGFTLAEARALSDQLKFGALPLSFVEQTSIDISPTLGSEQLR
                     YGFIAGFIGLFLVFVYSLLQYRVLGLVTVASILVAGLLTYLAITILGWSHNYRLDMAG
                     VTGLIVAIGFTADSFIVYFERIRDELRDGRSLAAAVETGWNRAKRTILIADGVNFLAA
                     AVLYALASSSVRGFAFTLGLTTLIDLLVVFWFTHPVVSLLARRPFFRDGHAWSGLDPR
                     RLTLSSAKPRYAGRGRFTHPTPAAAGTEGGVA"
     gene            complement(4068..4334)
                     /gene="yajC"
                     /locus_tag="PL014_09465"
     CDS             complement(4068..4334)
                     /gene="yajC"
                     /locus_tag="PL014_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT57361.1"
                     /translation="MDSGFGYVVILLLPFLLLVFMMYTQRKRAQQIQALQAALVIGDE
                     VVTTSGLYGTVVALDERVVTLDTGNGVTVRYDRRAIGSHASEVA"
     gene            complement(4500..5636)
                     /gene="ruvB"
                     /locus_tag="PL014_09470"
     CDS             complement(4500..5636)
                     /gene="ruvB"
                     /locus_tag="PL014_09470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT57362.1"
                     /translation="MSGGARERGFASEVVEADGSYEATARVVDPGRSEDDRVVEAALR
                     PRRLAEFPGQPRVRDQLGLVLEAARRRGRPPDHVLLSGPPGLGKTTLAMIVAAELEKP
                     LRLTSGPAIQHAGDLAAVLSSLDEGEVLFLDEIHRMSRSAEEMLYLAMEDFRVDVIVG
                     KGPGATAIPLELPPFTVVGATTRSGLLPAPLRDRFGFTAHLDYYDVADLVSILHRSAG
                     LLGVDADEEGIREIAGRSRGTPRIANRLLRRVRDWAQVHGEGHVDLAAAQVALALFDV
                     DAVGLDRLDRAVLEALCRRFGGGPVGLSTLAVAVGEEADTVETVAEPYLVREGYIVRT
                     PRGRAASPRAWTHLGITPPRSAPLQDLLPVDGDPDDTAAGRFGG"
     gene            complement(5633..6244)
                     /gene="ruvA"
                     /locus_tag="PL014_09475"
     CDS             complement(5633..6244)
                     /gene="ruvA"
                     /locus_tag="PL014_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT57363.1"
                     /translation="MIASVRGEVTHVGLDHVVVEVGGVGMFVHTTPTTASGCRRGAEV
                     SLATTLVVREDSLTLFGFASPAERDMFVTVQTVSGVGPRLALAMLSVMGPSELAAALA
                     SGDSKALTSIPGIGVKSAQRLVLELRDKVGAIPGAAPAAGAGSAVTRPGEEPWRAPVT
                     EALVGLGWSAKQSSDAVERVAASAPADADIAAYLRLALRELRP"
     gene            complement(6241..6756)
                     /gene="ruvC"
                     /locus_tag="PL014_09480"
     CDS             complement(6241..6756)
                     /gene="ruvC"
                     /locus_tag="PL014_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT57364.1"
                     /translation="MRVLGVDPGLTRCGLGVVDGRPGKAAMVAVGVVRTPSDGDPGER
                     LVYLERELDTWLGRYEPDVVAVERVFADTNITTVMTTAHATAVALLTATKRGIPVVFH
                     TPTEVKAAVTGSGRAGKAQVTAMVTRILGLEVAPRPADAADALALAICHAWRGAQLAR
                     LDALAAAQGRR"
     gene            complement(6866..>7548)
                     /locus_tag="PL014_09485"
     CDS             complement(6866..>7548)
                     /locus_tag="PL014_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT57365.1"
                     /translation="AKLIKNIEVAARTGGGDPAGNPTLYDAIQKAKKSSVPNSNIDNA
                     VKRGSGATAGGANWETITYEGYAPGGVAVLIECLTDNRNRAAAEVRTALTRNGGQLAD
                     PGSVSYVFDRKGVVIVPKGDKTEDDILEIVLEAGADEVNDLGEAFEVISEASDFVAVR
                     TALQEAGVDYDSAEASWVPNLQVPLDLDGAKKMIRVIEALEDSDDVQDVFANGDIPDD
                     VLAQLDEE"
BASE COUNT         1097 a         2495 c         2849 g         1107 t
ORIGIN      
        1 tgtgcaacga ctggtacatg ttgaacttcg gcatcgcgat gtagtccttg aaccgccccg
       61 gcaccgggtt ccaccgggcg tgcagcgccc ccagcgccgc gtagcagtcg cgcacggtct
      121 ccacgagtac gcgcacggcg acgaggtcgt agatctgctc gaagtcgcgg ccccccacga
      181 tcatcttctg gtacacggag tagtagtgct tgggccggcc ggtgacggtg gccttgatct
      241 tggccgtggc caggtcggcg ctcacctcgt cgcgcacggt ggcgaggtac tcctcgcgag
      301 ccggggcacg ctcggccacg agccggacga tctcgtcgta caccttcggg tacagcgcct
      361 ggaaggacag gtcctcgagc tcccacttga tggtgttcat gccgagccgg tgggccagcg
      421 gggcgtagat ctcgagggtc tccttggcct tgcgggcggc gctctcctgc gagacgtagc
      481 gccaggtgcg cgcgttgtgc agccggtccg cgagcttgat gacgaggacc cggatgtcgc
      541 gcgccatggc cacgaccatc ttgcgcacgg tctcggcctg cgcggcctgg ccgtaggtga
      601 ccttgtcgag cttggtcacg ccgtcgacga gcatggcgat ctcgtcaccg aagtcgcgcc
      661 gcaacgcctc gaggctgtac gacgtgtcct cgacggtgtc gtgcaacagc gccgcagcca
      721 acgtcgtcgg tgtcatgccg agctcggcga ggatcgtggt caccgccagg gggtgggtga
      781 tgtagtcatc gcccgatcgc cgcttctgcc cctcgtgcgc gcgctccgcg accgcgtagg
      841 cccgctcgat gacggcgacg tccgccttcg ggtgggttcc ccgcacgacc tggagcagcg
      901 gttcgaggac cgggttgcca ggggtgcggt tgcggcccac gagggccagg gccgcccggg
      961 ccctggcgcg cgaggacacg ccgggtgggg gtgtccccgc cccgacccac gtaccgtcct
     1021 cgaccatgcc gggagtgtag tgctcagcca gccggtcgcc gatgccggtc cacgatgctc
     1081 agcgcttgga cttcggcggg cgccgcggct ggttgcgcgg accggactgg gcgtacttgt
     1141 gcacggcccg gccggacagg gtcgatgcgt cgccggatgc cgcggcacgc gtgccgctgc
     1201 ccgcggacac cgcgacccgt gggtcctcgg cgccgccgtc accctcgccg aatgcctcag
     1261 cggtctcggc cgcggccacg tccttggccc gccgggcctg gtaccggctg gctcgcttgg
     1321 ccagttcgat gacggccggc tcgttgcgcc gcagggagac gagcaggggc gtggcgatga
     1381 agatcgagga gtaggcgccg acgatgatgc cgacgaagag cacgagcgag aggtcgagca
     1441 gcacgccggg cccgagccag atggcgccga ccgcgagcac ggcggccacg ggcagcacgc
     1501 cgatgatcgt ggtgttgatc gagcggacca tcgtctggtt gacggcgagg ttggcggcct
     1561 cggcatagga catgcggccg ttggcgaagg cctccgtggt gttctcgcgc accttgtcga
     1621 acacgacgac cgtgtcgtag aggctgtagc cgagcacggt gaggaagccg atcatcgtcg
     1681 cgggcgacac ctcgaacccg atcagggcgt agatgccgac ggtgatgatc atgtcgtgga
     1741 ccacggcgat cagcgccgcc gcggccatct tccagttgcg gaagtagatg ctcatgacca
     1801 cggccaccag ggcgaggaag atcaccaggg cgcgcagtgc ctgttgactg accgacgcac
     1861 cccacgatgg gccgatgaag gtgccgctga cctcagaggt cggcaccccg aaggcctcgg
     1921 cgaggttctg gcggacggct tggctgtcgg tgtcgttgag tcgttcggtc tgcacccgca
     1981 cggtgttgtt gccgacgacg gtgacgttga cggcacgctg gctctcagtt gcaccgatgg
     2041 cctggcgggc gatgtcctcg tacgtggcgg gcagcgacgt ggtcgccacc cggaactccg
     2101 aaccgccgcg gaactcgagc ccgaggttca ggccctggaa caccaggccg aggacggcga
     2161 tgagcatgac gacggctgag gcgatgtaga accggcgccg ttgggcgacg acgggcaccg
     2221 acttctcgcc ggtgtagagg tcgttgccga gcgtggcgaa gcgcgaactc atgccacacc
     2281 tccttcggtg cctgccgcgg caggagtggg gtgcgtgaaa cggccgcgac cggcataccg
     2341 gggcttggcc gaactcaggg tgaggcgacg tggatccagg ccggaccagg cgtggccgtc
     2401 acggaagaac gggcgccggg ccagcagcga gacgaccggg tgggtgaacc agaagacgac
     2461 caggaggtcg atgagcgtgg tcaggcccaa ggtgaacgcg aagccgcgca cgctcgagct
     2521 ggcgagcgcg tagagcacgg cggcggcgag gaagttcacg ccgtcggcga tgaggatggt
     2581 gcgcttggcg cggttccacc cggtctcgac ggcagcggcg agggagcgcc cgtcacgcag
     2641 ttcgtcacgg atgcgctcga agtacacgat gaacgagtcc gcggtgaacc cgatggcgac
     2701 gatgaggccg gtcacgccgg ccatgtcgag gcggtagttg tgcgaccacc cgaggatcgt
     2761 gatggcgagg taggtcagca ggcccgcgac gaggatcgag gccaccgtga ccagacccag
     2821 gacgcggtac tggagcaggg agtacacgaa cacgaggaac agtccgatga agccggcgat
     2881 gaacccgtac cgcaactgct cgctgcccag cgtggggctg atgtcgatgc tcgtctgctc
     2941 gacgaaggac aacggcagcg caccgaactt cagctggtcg gacagggctc gggcctcggc
     3001 cagggtgaag ccaccggtga tgctggcgcg gccgtcgagg atggcctcgt tgaaccgggg
     3061 ggcgacgatg acccgggagt ccagggtcgc ggcgagctgg ttctgcggct cgggcagggc
     3121 gaccatggcg cggctgatgt cggcgtactg cttggcaccc tcgctggtga gggtcagggc
     3181 gatctcgaca tcgttggtca cgacaccctg ctggttggtg cggtaccccg aggtggcgtc
     3241 actgatcttg tcgccggcga cggcgaccgg accgaggatg tacttgacgc tgtcgtcgtc
     3301 ctgacacgtg acgagcggct tggccgggtc gtcgatgagg ttgtccagcg ccgcggggtc
     3361 agcacagttg aggagctcga actgggtgct cagctcggga gtgatccacg cgaggtccgt
     3421 cgggctggtc ggggaggcga cagatgccgc cgggctgggc gtcggcgacg ggctggcggt
     3481 cgcactcggc gacggggtcg gggccatgag cgcctcgggg accgagctgt tgttcgtcgg
     3541 ggaggcgctg ctcgccacgg tcggggtcgg cgacgggctc gcggccgggg ccccggacgc
     3601 tgccggggac gcgctggggt cgggggatgc cgtgccgctc ggggacaccg tcggctgcgg
     3661 ctgcgggctt ccggcggcga tcgcgaggac gggacggaac tgcagctggg aggacttgct
     3721 gatcgcgtca cgcacctcct gcgtcgggat ctcggggatg ctgacgacga tgttgcggtc
     3781 accctgggtc gtcacctcgg cgccggagac accaccggcg tcgacgcgct ggacgatgat
     3841 gttgcgtgcc tggttgagct gctcggccgt gacggtctgg ccctcgacgg tcttgggctc
     3901 gaggatgatc tgcgtcccgc ccttgaggtc cagcccgagc ttcggggtgg cctgggcgtc
     3961 cgaccagtgc atggcaccgg cgagcagccc catgagggcg agcgacagga tggtgagggc
     4021 gagcagggct cggcgcgcgg cggagcgctg ggtcttcaac gacatgatca ggcgacctcg
     4081 gaggcgtgcg aaccgatggc gcggcggtca tagcgcaccg tgacaccgtt gccggtgtcc
     4141 agcgtgacga cgcgctcgtc gagcgccacg accgtcccgt agaggccgga ggtcgtgacg
     4201 acctcgtcgc cgatgaccag cgcggcctgg agggcctgga tctgctgggc ccgcttgcgc
     4261 tgggtgtaca tcatgaacac cagcagcagg aacgggagca gcaggatgac gacgtagccg
     4321 aagccggagt ccatgggtga gatcggtcct tagtggtcag ggtgcccgct cgtggcaggc
     4381 aggggtgcgg cgggggcgca cccgggccaa cgcccgagtc taggtgacgg gttccacccg
     4441 ctccaacgcc agcaccctcc cggcgtggct cgtgcggtgg gcggcacgtc ggcgcgtgct
     4501 cagccgccga accggcctgc ggccgtgtcg tcggggtcac cgtcgaccgg tagcaggtcc
     4561 tggagcggtg cgcttcgtgg cggggtgatg cccaggtggg tccacgcccg cggcgaggca
     4621 gcccgcccgc gcggggtgcg cacgatgtag ccctcgcgca cgaggtaggg ctcggcgacc
     4681 gtctcgacgg tgtcggcctc ctcccccacg gcgacggcca gggtcgacaa tccgaccggc
     4741 ccgccgccga accggcggca cagcgcctcg agcacggccc tgtccagccg gtcgaggccg
     4801 acggcgtcga cgtcgaagag cgccagggcg acctgcgcgg ccgcgaggtc gacgtggccc
     4861 tcgccgtgca cctgggccca gtcgcgcacc cggcgcagca ggcggttggc gattcggggg
     4921 gtcccccgag agcgaccggc gatctcgcgg atgccctcct cgtcggcgtc gaccccgagc
     4981 aggccggcag agcggtgcag gatcgacacg aggtcggcga cgtcgtagta gtcgaggtgg
     5041 gcggtgaagc cgaaccggtc acgcagcggc gccggaagca gccccgagcg ggtggtggcg
     5101 ccgaccacgg tgaacggcgg aagctcgagc gggatcgcgg tggccccggg ccccttgccg
     5161 acgatgacgt cgacgcggaa gtcctccatc gcgaggtaga gcatctcctc ggcggagcgg
     5221 ctcatccggt ggatctcgtc gaggaagagc acctccccct catcgagcga ggagagcacc
     5281 gccgccaggt ctccggcgtg ctggatggcc gggccgctcg tgagccgcaa gggcttctcg
     5341 agctcggccg cgacgatcat cgccagggtg gtcttgccca acccgggcgg gcccgagagc
     5401 aggacgtggt ccggtggccg gccgcgccgc cgggccgcct cgagcaccag cccgagctgg
     5461 tcacgcacgc gtggctgacc cgggaactcc gcgagacggc gggggcgcag cgcggcctcg
     5521 acgacccggt cgtcctcgga gcgtccgggg tccaccacgc gggccgtggc ctcgtaggac
     5581 ccgtccgcct cgacgacctc gctggcgaag ccgcgctcgc gcgccccgcc gctcacggcc
     5641 gcagctcccg cagggccagc cgcaggtagg cggcgatgtc ggcgtcagcc ggcgcggacg
     5701 cggcaacccg ctccacggca tccgaggact gcttggcaga ccagcccagc ccgaccaggg
     5761 cctcggtcac cggtgccctc cagggctcct caccggggcg ggtcaccgcc gagcccgcgc
     5821 ctgcggctgg tgcggcacct gggatggcgc cgaccttgtc gcgcagttcg agcacgagcc
     5881 gctgcgccga cttcacgccg atgccgggga tcgacgtcag cgccttgctg tcgccgctgg
     5941 ccagggctgc ggccagctcg ctcgggccca tgaccgacag catcgccagg gccagccggg
     6001 gcccgacccc ggagacggtc tggacggtga cgaacatgtc gcgctctgcg ggcgaggcga
     6061 agccgaagag cgtcagggag tcctcgcgca cgacgagcgt cgtcgcgagg gagacctcgg
     6121 cgccccgacg gcatccggat gccgtggtgg gcgtggtgtg cacgaacatg ccgacgccgc
     6181 ccacctcgac gacgacgtgg tcgagaccga cgtgggtgac ctcaccacgc accgaggcga
     6241 tcatcgccgc ccctgcgccg ccgcgagggc gtcgaggcgg gcgagctggg cgccgcgcca
     6301 cgcgtggcag atcgcgaggg ccagggcgtc cgcggcatcc gcggggcgcg gcgcgacctc
     6361 gaggccgagg atgcgggtga ccatggcggt gacctgagcc ttgcctgcgc ggccggagcc
     6421 cgtcacggcg gccttgacct cggtcggggt gtggaagacg acggggatgc cgcgcttggt
     6481 cgccgtgagc agcgcgaccg cggtggcgtg ggccgtggtc atgacggtgg tgatgttggt
     6541 gtcggcgaag acccgctcga cggccacgac gtcgggttcg tagcggccca gccaggtgtc
     6601 gagctcgcgc tcgaggtaga ccagccgctc ccccgggtcg ccgtcgctcg gggtccgtac
     6661 gacaccgacg gcgaccatcg cggccttgcc ggggcgaccg tcgacgacgc cgagaccaca
     6721 gcgggtcagg ccggggtcga ctccgagcac gcgcacgagc ggggtccctt cgacggtggg
     6781 cggcggttta gaacacctgt tcgggacgac acgctacaca gcgaaggccc gcggggtgtg
     6841 cagcaacacg cgcagcccgc ctcgctcact cctcgtcgag ctgggcgagc acgtcgtccg
     6901 ggatgtcacc gttggcgaag acgtcctgca cgtcgtcgga gtcctcgagg gcctcgatga
     6961 cgcggatcat cttcttggcc ccgtcgaggt cgagcggcac ctggaggttc ggcacccacg
     7021 aggcctcggc cgagtcgtag tcgaccccgg cctcctgcag cgccgtgcgc acggcgacga
     7081 agtcactggc ctcggagatg acctcgaagg cctcccccag gtcgttgacc tcgtcggccc
     7141 cggcctcgag gacgatctcg aggatgtcgt cctcggtctt gtcccccttg ggcacgatga
     7201 cgaccccctt gcggtcgaac acgtagctca ccgatcccgg atcggcgagc tggccgccgt
     7261 tgcgggtcag ggcggtgcgg acctcggcag ccgcccggtt gcggttgtcg gtgaggcact
     7321 cgatgagcac cgcgacgccg ccgggggcgt atccctcgta ggtgatggtc tcccagttgg
     7381 ccccacccgc ggtggcgccc gacccgcgct tgaccgcgtt gtcgatgttg ctgttgggga
     7441 cggaggactt cttggccttc tggatcgcgt cgtagagggt cgggttgccc gcagggtcac
     7501 cgccaccggt gcgcgcggcc acctcgatgt tcttgatgag cttggcga
//
LOCUS       DAOSOC010000123         7484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_9876_length_7484_cov_11.172836, whole genome shotgun sequence.
ACCESSION   DAOSOC010000123 DAOSOC010000000
VERSION     DAOSOC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9876_length_7484_cov_11.172836"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..794
                     /locus_tag="PL014_09490"
     CDS             186..794
                     /locus_tag="PL014_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57366.1"
                     /translation="MRTTRAATARGASTPAGQPAARAPRRAASVALVAVVALAVSACG
                     NDPEPEVIGAPIVLSGSPANDDATASGTPSPTPTASAQSGGAAPVVAVVRDDDAEVDV
                     DDQRGDGTTVLVREVRLPTTDGHVVVIDPRSRAVLGSALVRAGTTRPVTIDLTTPVQV
                     SGELVALLYADDGDGRFDPTTDAGVVDDDGDAVDDDFEFTVG"
     gene            787..2199
                     /locus_tag="PL014_09495"
     CDS             787..2199
                     /locus_tag="PL014_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOT57367.1"
                     /translation="MAESAARSWRPRDSARARLLLVQLALVALALLVSLVITRQYLMA
                     RVDDRIDATLTQEVDEFRALAQTGIDPVTRAPFDDPRRLLQLHLQRSIPQTNETLIGL
                     VDGVPVSRVAQEPPARLDLDPDAVAAFAAAQDTRTGQVRTERGVVTYRVVPVLVEGST
                     TRGALVVGVFRDLERREVDVVTYVLLGAGAVGLLIASALAWVAAGRVLRPVDDVRRAA
                     EEIGASDLSRRLEVRGTDEVAQLAGTFNGMLDRLEHAFADQRQFLDDASHELRTPITI
                     VRGNLETLSEVPAERAASMAVVLDELDRMGRLVEDLLTLARSDGPDFVVLGSVELSEL
                     TLGVLTRARSLADRDWQLDEVGAGFFSADEQRVLQAMLQLAENAVAHTEPGDLIRIGS
                     AISGDSVDLWVHDSGPGIAPEQRARVLERFVRAGTSRADGAGLGLAIVAGIARAHHGE
                     VVIDDTVAGARVRLHLPVRG"
     gene            2204..2884
                     /locus_tag="PL014_09500"
     CDS             2204..2884
                     /locus_tag="PL014_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT57368.1"
                     /translation="MARILIVEDETRISDFLDRGLRAAGHTTSVAADGTTGLHLALAG
                     DIDLVVLDLGLPDRDGLEVLADLRGSGSDVPVIVLTARGGVRDTVAGLTTGATDYVVK
                     PFHFAELEARVALRLAESRRVGAGSPRADDTRLTAGDVELDLLERRLTVGGADHDLTE
                     REFQLLETLMRHAGQVLSREQLLDLVWGFDFAPGSNVVDVYVAQLRSKVGRDRIETVR
                     GAGYRLRR"
     gene            2968..3354
                     /locus_tag="PL014_09505"
     CDS             2968..3354
                     /locus_tag="PL014_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57369.1"
                     /translation="MSAHVVEAVGVYAADGGVVGELTYVVGHLLGRTECSLCDVTHGP
                     VRRKRAWDEMAAVLPVHVRLVHRNETTPDEAAACAVSGLPVVLGVRADGSHTTLLGPR
                     ELKALDGSVDAFGTAMRAALARVETS"
     gene            3351..3863
                     /locus_tag="PL014_09510"
     CDS             3351..3863
                     /locus_tag="PL014_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HOT57370.1"
                     /translation="MSHPRPYPVGPGQESVWDYPRPPRVEASAEHVQVWFGGVLIADT
                     TASLRVLETSHPPTYYLPEGAFVAGALRPTAGSTWCEFKGVAAYFDVVGGDAVGGGAV
                     ALRAAWAYPRPSPGFEALVGHVALMPGAMDRCLVDGEVVTAQAGGFYGGWITSRVVGP
                     FKGEPGTRGW"
     gene            complement(3916..4551)
                     /locus_tag="PL014_09515"
     CDS             complement(3916..4551)
                     /locus_tag="PL014_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOT57371.1"
                     /translation="MEFDVTIEIPKGQRNKYEVDHESGRIRLDRMLFTAMAYPSDYGY
                     IEDSLGEDGDPLDALVLLDEPTFPGCLVRARPIGMFHMRDEAGGDDKILCIPAGDPRK
                     YHIREIEDVSEFDRLEIQHFFETYKDLEPGKSVEGAHWAGRDEAENCIREAITRAKEA
                     GISTARWRMPEHDDLVWDGGSSQRTPEEISAASERARNELATKENPLSDNA"
     assembly_gap    4675..4774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4775..5629
                     /locus_tag="PL014_09520"
     CDS             <4775..5629
                     /locus_tag="PL014_09520"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOT57372.1"
                     /translation="KVTLRLDLSWAPGPRYPPEWNPDDVRDGFTQAVVMTGLATQLPA
                     AGRPSPIRPETEVAKAFVAALKAKGVSATLAPDKTWGDPASEDAVELGSVRSATYGEV
                     LDLALDRSENALTENLVRQAAATAGRSTTKGGDNAAFVVERLTAHGIPTEGLVLKDAS
                     GLSPGQAASAATLSGVLHLAATDSPDQVPGLRDVIAGLPVSGVSGTMRGRFSSDATED
                     VVGVPRAKTGTLKAGSALAGTTVTADGRPLTFVVLVDEFPEDFGGTLRARAALDRIVA
                     ALTRCGCR"
     gene            5658..6710
                     /locus_tag="PL014_09525"
     CDS             5658..6710
                     /locus_tag="PL014_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOT57373.1"
                     /translation="MGYVDWEFAKTTGRKLVAAGPAVTHEEAVAEVEAIRAAAAAARE
                     PVAETARLRTPDDAPDALVVDRATWIAVNADSMSAMLDPTFEEIIAKRGGGESATPNA
                     TVAAVGAKVTGAEAGALLAFMASKVLGQYDLAPGGKPALMLVAPNIIDVASTLGVDRA
                     DFRAWVCMHEETHRVQFTANPWLREHLIAQARELAVDLAPDSERLGELTELLTQRLPE
                     VFREGSTGLTGLFTTPEQREKMADLTAVMSLLEGHADVVMDDVGPAHIPTVAHIRAKF
                     SEKRKGAGAPDRMLRRLLGLEAKLRQYRDGAVFVRGVQKSVGVDGFNAIWTSPETLPL
                     AREIEDPQAWVRRVHG"
     gene            6736..>7484
                     /gene="tilS"
                     /locus_tag="PL014_09530"
     CDS             6736..>7484
                     /gene="tilS"
                     /locus_tag="PL014_09530"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT57374.1"
                     /translation="MSRPWGKPDAATAAIRVAVRRTLAELSSSPASGTGPPAGTAADA
                     VAPPLVLVACSGGADSLALAAATAFEGPAAGIRAGAVVVDHGLQPASAEVAARAAATL
                     ITLGLGPVEMIRVEVTTDGAGVEAAARAARYRALEEAADRHGAALVLLGHTLDDQAEQ
                     VLLGLVRGSGARSLAGMPPARGRFRRPLLGTTRAQCRASVTAHGLTWWDDPMNEDPTF
                     ARVRARRAVADLERDLGPGVAAALARTASHLR"
BASE COUNT          965 a         2672 c         2698 g         1049 t
ORIGIN      
        1 cagagcgatt cactcgtaat gaataggtca tcggttcgat tccgataggc ggctcagatg
       61 atgccctcga ctccggtcgg gggcatttct gctgctcggg gtgaaccggc gaaaccgccc
      121 gcgcatgaag gatccttcat gaagcactca cgctgcgctc acccggacga ggcacgatgg
      181 tccccatgcg cacgacccga gctgccaccg cccgtggagc gagcactccc gccgggcagc
      241 ctgctgcgcg agcccctcga cgcgccgcat cggtggccct cgtggccgtc gtggcgctcg
      301 cggtcagcgc ctgcggcaac gaccccgagc ccgaggtcat cggtgccccc atcgtgttgt
      361 ccggctcgcc agcgaacgac gacgcaacgg catccggcac cccgtcgccc acgccgacgg
      421 cctccgcgca gtccggaggc gctgccccgg tcgtggctgt cgtgcgagac gacgacgccg
      481 aggtggacgt cgacgaccag cgcggcgacg gcaccacggt tctcgtccgg gaggtacgcc
      541 tgcccaccac cgacgggcac gtcgtcgtca tcgacccccg gagtcgagcg gtgctcggca
      601 gcgccctggt gagggccggc accacgcgcc ccgtcacgat cgacctgacc acccccgtgc
      661 aggtgagcgg tgagctggtc gccctcctgt acgccgatga cggtgacggc cggttcgacc
      721 cgaccaccga cgccggggtc gtcgatgacg acggcgacgc ggtcgacgac gacttcgagt
      781 tcaccgttgg ctgagtcagc cgcgcggtcc tggcgccctc gcgactcggc cagggcccgg
      841 ctgttgctcg tccagctggc cctggtcgcg ctggccctgc tcgtcagcct cgtcatcacc
      901 cgccagtacc tcatggcgcg cgtcgacgac cgcatcgacg ccaccctgac gcaggaggtc
      961 gacgagttcc gcgcgctggc ccagaccggc atcgaccccg tgacccgggc accgttcgac
     1021 gacccccgac ggctcctcca gctgcacctc cagcgcagca tcccccagac gaacgagacc
     1081 ctcatcgggc tggtggacgg ggtgccggtc agccgcgtcg cgcaggagcc gccggcccgt
     1141 ctggacctcg atccggatgc cgtggccgcc ttcgccgccg cccaggacac ccgcaccggt
     1201 caggtccgga ccgagcgcgg cgtggtcacc taccgcgtcg tcccggtgct cgtcgagggc
     1261 tcgacgacgc gtggggcgct ggtggtgggc gtgttccgcg acctggagcg gcgcgaggtc
     1321 gacgtcgtca cgtacgtgct cctcggtgcg ggtgccgtcg gactcctcat cgccagcgcc
     1381 ctggcctggg tggccgcggg ccgggtgctg cgaccggtgg atgacgtgcg ccgcgcggcc
     1441 gaggagatcg gtgcctccga cctgtcgcgg cgcctcgagg tgcgcgggac cgacgaggtc
     1501 gcccagctcg cgggcacgtt caacggcatg ctcgaccggc tcgagcacgc cttcgccgac
     1561 cagcggcagt tcctcgacga cgccagccac gagctgcgca cgcccatcac catcgtgcgg
     1621 ggcaacctcg agaccctgag cgaggtgcca gccgagcgcg cggcatccat ggccgtggtc
     1681 ctcgacgagc tcgaccgcat ggggcgcctc gtcgaggacc tgctcaccct cgcccgctcg
     1741 gacggcccgg acttcgtcgt cctcgggtcg gtcgagctga gcgagctcac cctcggcgtg
     1801 ctcacccgcg cgcgatcgct cgccgaccgc gactggcagc tcgacgaggt cggtgccggg
     1861 ttcttcagtg ccgacgagca gcgcgtgctc caggccatgc tccagctcgc cgagaacgcc
     1921 gtggcccaca ccgaacccgg cgacctcatc cggatcggca gcgcgatcag cggcgactcg
     1981 gtcgacctgt gggtgcacga cagcggcccg ggcatcgccc ccgagcagcg ggcccgggtg
     2041 ctcgagcgct tcgtgcgggc cggcacgtca cgcgccgacg gcgcgggcct cggtctcgca
     2101 atcgtcgcgg gtatcgcgcg ggcccaccac ggtgaggtcg tcatcgacga cacggttgcc
     2161 ggggcgcggg ttcgcctgca cctcccggtg agagggtgat ccggtggcac gcatcctgat
     2221 cgtcgaggac gagacgcgca tctccgactt cctcgaccgc ggcctgcgcg cggccgggca
     2281 cacgacctcg gtcgctgcgg acggcaccac gggcctgcac ctggccctgg ccggcgacat
     2341 cgacctggtc gtgctggacc tcggccttcc ggaccgcgac gggctcgagg tcctggccga
     2401 cctgcggggg agcggcagtg acgtccccgt catcgtgctg acggcccggg gaggggtgcg
     2461 cgacaccgtc gcgggcctca ccaccggcgc cacggactac gtcgtgaagc cgttccactt
     2521 cgccgagctc gaggccaggg tggcgctgcg cctggccgag tcgcggcggg tgggcgctgg
     2581 ctccccgcgg gccgacgaca cgcggctgac ggccggtgac gtcgagctcg acctgctcga
     2641 gcggcggctg acggtggggg gcgcggatca cgacctgacc gagcgcgagt tccagctgct
     2701 cgagaccctg atgcgccacg ccggccaggt gctctcgcgc gagcagctgc tggacctcgt
     2761 gtggggcttc gacttcgccc cggggtcgaa cgtcgtcgac gtctacgtcg cccagctgcg
     2821 ctccaaggtc ggccgcgacc ggatcgagac ggtgcggggc gcgggctacc gcttgcgacg
     2881 ctgaccgcac ccacggcatc cgccctgctg agcggggttt gtcgaagcct gaacgggccg
     2941 cagggcgctg cggattagcg ttgggccatg tcagcgcacg tggtcgaggc cgtcggggtg
     3001 tacgccgccg acggcggggt cgtcggtgag ctcacctacg tcgtcgggca cctgctcggc
     3061 cgcaccgagt gctcgctgtg cgacgtcacg cacggccccg tgcggcgcaa gcgggcctgg
     3121 gacgagatgg ccgcggtcct gcccgtgcac gttcgcctgg tccaccgcaa cgagacgacg
     3181 cccgacgagg ccgctgcgtg cgcggtcagc ggcctacccg tggtgctcgg ggtgcgggcc
     3241 gacggcagcc acacgaccct gctcggaccc cgtgagctca aggccctcga cggctcggtc
     3301 gacgcgttcg ggaccgccat gcgggccgcg ctggcccggg tggagacgtc atgagccacc
     3361 cgaggccgta cccggtcggg ccggggcagg agtccgtgtg ggactacccg cgcccacccc
     3421 gggtcgaggc cagcgccgag cacgtgcagg tctggttcgg cggagtactc atcgccgaca
     3481 ccacggcgtc gctgcgcgtg ctcgagacca gccacccgcc gacctactac ctgcccgagg
     3541 gagcgttcgt ggcgggggcg ttgcgtccca cggccggctc gacctggtgt gagttcaagg
     3601 gcgtcgccgc gtacttcgac gtcgtcggtg gggatgccgt cggtgggggt gccgtggcac
     3661 tgcgcgccgc gtgggcgtac ccccgcccca gccccggctt cgaggcgctc gtcggtcacg
     3721 tggcactcat gccgggcgcc atggatcgct gcctcgtcga cggcgaggtc gtgaccgccc
     3781 aggctggtgg gttctacgga ggctggatca cctcacgggt ggtcggcccc ttcaagggag
     3841 aacccggcac ccgagggtgg tagccggggc cccgccgggc cgttcacggc cggccccgcc
     3901 caccctggtc ggtgctcagg cgttgtcgct gagcgggttc tccttggtgg cgagctcgtt
     3961 gcgggcgcgc tcggacgctg ccgagatctc ctcgggggtg cgctgcgagg agccgccgtc
     4021 ccagacgagg tcgtcgtgct cgggcatgcg ccagcgggcc gtggagatgc cggcctcctt
     4081 ggcgcgggtg atggcctcac gaatgcagtt ctcggcctcg tcgcggcccg cccagtgggc
     4141 gccctcgacc gacttgccgg gctcgaggtc cttgtaggtc tcgaagaagt gctggatctc
     4201 gaggcggtcg aactcgctga cgtcctcgat ctcgcggatg tggtacttgc gcgggtcgcc
     4261 cgccgggatg cacaggatct tgtcgtcacc gccggcctcg tcacgcatgt ggaacatgcc
     4321 gatcgggcgg gcgcgcacga ggcagccggg gaaggtcggc tcgtcgagca gcaccagggc
     4381 atcgagcggg tcgccgtcct caccgaggga gtcctcgatg tagccgtagt ccgaggggta
     4441 ggccatcgcg gtgaacagca tgcggtccag gcggatgcgc ccggactcgt ggtcgacctc
     4501 gtacttgttg cgctgcccct tggggatctc gatggtgacg tcgaactcca cgacgggctc
     4561 tccttgcgtg agggtgctgg gggtgacagg cggttgacct aggctctttg cacagtgtgg
     4621 cccataggcg gggtcgaggg cgaagggagt ggccgtgaga cgagtcatgc tcgcnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaggtc accctgcgcc tcgacctcag
     4801 ctgggcgccc gggccgcggt acccaccgga gtggaacccc gatgacgtgc gcgacggctt
     4861 cacccaggcc gtcgtcatga ccgggctcgc gacacagctg cccgctgcgg ggcgcccgtc
     4921 accgatccgc cccgagacgg aggtggcgaa ggccttcgtc gcagcgctca aggccaaggg
     4981 ggtgagcgcc accctggcgc ccgacaagac ctggggcgac ccggcatccg aggatgccgt
     5041 cgagctgggc agcgtgcgct cggccaccta cggcgaggtg ctcgacctcg cgctggaccg
     5101 cagcgagaac gccctgaccg agaacctcgt gcgccaggcc gccgcgacgg cgggtcggag
     5161 cacgaccaag ggcggcgaca acgccgcgtt cgtcgtcgag cggctgacgg cgcacggcat
     5221 cccgaccgag gggctcgtgc tcaaggacgc cagcgggctc agccccggtc aggcggcgag
     5281 tgcggccacc ctgtcgggcg tgctgcacct ggccgcgacc gacagcccgg accaggtgcc
     5341 cggcttgcgc gacgtcatcg cggggctccc ggtcagcggc gtgtccggca cgatgcgcgg
     5401 gcgcttctcc tccgacgcca ccgaggacgt cgtgggggtg cctcgtgcca agacgggcac
     5461 cctgaaggcg gggtcggcgc tcgcgggaac gacggtcacc gccgacggcc gcccgctgac
     5521 gttcgtcgtg ctcgtcgacg agttccccga ggacttcggg ggaacgctgc gtgctcgggc
     5581 cgcgttggac cggatcgtgg ccgccctgac caggtgcggc tgccgctgac gcggcacaac
     5641 ggagaggctg ggcccacgtg ggatacgtcg actgggagtt cgcgaagaca accgggcgaa
     5701 agctggtggc cgccgggcct gccgtcaccc acgaggaagc cgtcgccgag gtcgaggcca
     5761 tccgcgctgc cgctgctgcg gcgcgggagc cggtcgccga gaccgcccgg ctgcgcaccc
     5821 ccgatgacgc gccggatgcc ctcgtcgtcg accgggccac gtggatcgcc gtcaacgccg
     5881 actcgatgag cgccatgctc gacccgacct tcgaggagat catcgccaag cgtggcggcg
     5941 gcgagtcggc cacacccaat gcgaccgtcg cggcggtcgg ggccaaggtc accggtgcgg
     6001 aggccggtgc cctgctcgcg ttcatggcga gcaaggtgct ggggcagtac gacctcgcgc
     6061 ccggcggcaa accggccctc atgctcgtcg ccccgaacat catcgacgtc gcctcgaccc
     6121 tcggggtcga ccgcgcggac ttccgcgcct gggtctgcat gcacgaggag acccaccggg
     6181 tgcagttcac cgccaacccc tggctgcgcg agcacctcat cgcgcaggct cgggagctgg
     6241 ccgtcgacct cgcgcccgac tccgagcgcc tcggtgagct gactgaactg ttgacccagc
     6301 gcctgcccga ggtcttccgc gagggcagca ccggtctcac cgggctgttc acgacacccg
     6361 agcagcgcga gaagatggcg gacctcaccg ccgtcatgtc gctgctcgag gggcacgccg
     6421 acgtggtcat ggacgacgtc ggcccggccc acatcccgac cgtcgcgcac atccgggcga
     6481 agttcagcga gaagcgcaag ggtgccgggg ccccggaccg gatgctgcgg cgcctgctcg
     6541 gcctcgaggc caagctgcgt cagtaccgcg acggtgcggt gttcgtgcgc ggggtgcaga
     6601 agtccgtcgg ggtggacggg ttcaacgcga tctggacctc accggagacc ctgccgctcg
     6661 cccgcgagat cgaggacccg caggcctggg tccgtcgcgt ccacggctga cccgctgggt
     6721 cgggaggatg gggtcgtgag tcgaccgtgg ggcaagccgg acgccgcgac tgcggcgatt
     6781 cgggtcgccg tccgtcgcac cctcgctgag ctgtcctcgt ccccggcctc gggcaccggg
     6841 ccccccgctg ggacggcggc ggatgccgtt gctccccctc tcgtgctcgt cgcgtgcagc
     6901 ggtggtgcgg actccctggc gctggctgcg gcgacggcgt tcgaggggcc agcggcgggg
     6961 atccgcgcgg gggcggtggt ggtcgaccac ggcctgcagc cagcctccgc cgaggtcgca
     7021 gcccgggccg cggcgaccct gatcaccttg gggctgggcc cggtcgagat gatccgcgtc
     7081 gaggtcacca ccgacggtgc gggtgtcgag gcggccgccc gggcagcccg ctaccgcgcg
     7141 ctggaggagg ccgccgaccg gcacggcgcc gccctcgtgc tgctcgggca caccctcgac
     7201 gaccaggccg agcaggtgct cctgggcctg gtgcgcggat ccggtgcccg atccctggcg
     7261 gggatgccgc ccgcgcgcgg ccgcttccgc cgcccgctgc tgggcaccac tcgcgcccag
     7321 tgccgggcct cggtcacggc gcatggcctg acctggtggg acgacccgat gaacgaggac
     7381 cccaccttcg cgcgggtgcg ggcgcgtcgg gcggtcgctg atctcgagcg cgacctcggg
     7441 ccgggggtcg ccgcggcact ggcgcgaacg gcatcccacc ttcg
//
LOCUS       DAOSOC010000124         7409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_10026_length_7409_cov_11.711857, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000124 DAOSOC010000000
VERSION     DAOSOC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10026_length_7409_cov_11.711857"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1395
                     /gene="tuf"
                     /locus_tag="PL014_09535"
     CDS             199..1395
                     /gene="tuf"
                     /locus_tag="PL014_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOT57375.1"
                     /translation="MAKAKFERTKPHVNIGTIGHIDHGKTTLTAAISKVLHDKYPDLN
                     PQFAFDDIDKAPEEKQRGITISIAHIEYQTEGRHYAHVDCPGHADYVKNMITGAAQMD
                     GAILVVAATDGPMPQTKEHVLLARQVGVPYIVVALNKADMVDDEEILELVEMEVRELL
                     STYEFPGDDLPVVKVSALKALEGDPEWGATVLELMDAVDDYIPEPVRETDKPFLMPVE
                     DVFTITGRGTVVTGRIERGILKVNEEVEIVGIHEGPATKTVATGIEMFRKLLDEGRAG
                     ENVGLLLRGIKREDVERGQVVVKPGSITPHTGFDANVYILSKDEGGRHTPFYDNYRPQ
                     FYFRTTDVTGVVKLPEGTEMVMPGDNTEMAVELIQPIAMEEGLKFAIREGGRTVGAGR
                     VTKITK"
     gene            complement(1597..2385)
                     /locus_tag="PL014_09540"
     CDS             complement(1597..2385)
                     /locus_tag="PL014_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT57376.1"
                     /translation="MSETPEGAVLATDHALALRLVTECGDLAQQMHAAGVDTEYKSSL
                     SDVVTAADRACEALVVSVTQQERPSDGVLGEEGASLRGTSGRRWVIDPVDGTHNFTMG
                     IPYWCSALALEGSDGALLGAVYQPSTADVWLGGAGAPTTRNGVPVAALVPRRLDECVL
                     ATYLHPATLRDPNLAEPWFAIVGDAATARMFGSGSCDLASVASGRMDLWVQHSTAEWD
                     WLPGRALVDAAGGRTAVVRHRGHTWHIAGNPRAVGDVLDVLRLS"
     gene            complement(2493..3092)
                     /locus_tag="PL014_09545"
     CDS             complement(2493..3092)
                     /locus_tag="PL014_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57377.1"
                     /translation="MPVSPTSLKIAAVSGLIAIGAGVGVATASAETTPSPSSTSSSTA
                     SSGAAAANQGAASTSTDDTGPRGGQKGGRGMDSAALASALALDEAKVTAAVSAAREAT
                     RPDAPAQGETRTPPTEAERTARQKAFATALAKELGIDEAKVTAALEKVKAAHEAEHKT
                     ELTARLDAAVTAGDLTASDKASVLKAFDAGVLQGGPGPR"
     gene            3572..3880
                     /gene="rpsJ"
                     /locus_tag="PL014_09550"
     CDS             3572..3880
                     /gene="rpsJ"
                     /locus_tag="PL014_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOT57378.1"
                     /translation="MAGQKIRIRLKSYDHEVIDSSARKIVDTVTRAGATVVGPVPLPT
                     EKNVFCVIRSPHKYKDSREHFEMRTHKRLIDIIDPTPKAVDSLMRLDLPADVNIEIKL
                     "
     gene            3891..4544
                     /gene="rplC"
                     /locus_tag="PL014_09555"
     CDS             3891..4544
                     /gene="rplC"
                     /locus_tag="PL014_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOT57379.1"
                     /translation="MTTSTKTQKGLLGTKLGMTQVWDENNRLVPVTVIQAGPNVVTQV
                     RDAETDGYAAVQLGYGAIDPRKVTKPLTGHFEKAGVTPRRHVVELRTADAGDYSLGQE
                     LGADTFEVGQSVDVTGTTKGKGFAGVMKRHGFKGVSSSHGSHRNHRKPGSIGGASTPG
                     RVFKGMRMAGRMGGVRQTTQNITVHAVDAEKGLLLLKGAVPGPRGAVVLVRSAAKGA"
     gene            4550..5134
                     /gene="rplD"
                     /locus_tag="PL014_09560"
                     /pseudo
     CDS             4550..5134
                     /gene="rplD"
                     /locus_tag="PL014_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
     gene            5365..5664
                     /gene="rplW"
                     /locus_tag="PL014_09565"
     CDS             5365..5664
                     /gene="rplW"
                     /locus_tag="PL014_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOT57380.1"
                     /translation="MSQGKNPRDILIAPVVSEKSYGLLDQGKYTFIVDPRSNKTEIKI
                     AVEQIFNVKVDSVHTMNRVGKARRTRFGIGKRKDTKRAIVTLREGSIDIFGSAGA"
     gene            5683..6519
                     /gene="rplB"
                     /locus_tag="PL014_09570"
     CDS             5683..6519
                     /gene="rplB"
                     /locus_tag="PL014_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOT57381.1"
                     /translation="MGIRKYKPTTPGRRGSSVADFVEVTRSTPEKSLVRPLTKTGGRN
                     NNGRITTRHIGGGHKRAYRVIDFRRHDKDGIPAKVAHIEYDPNRTARIALLHFADGEK
                     RYILAPKGLEQGMQIEHGPSADIKPGNNLPLRNIPTGTVIHAVELKPGGGAKIARSAG
                     VRVQLVAKDGPYAQLRMPSGEIRNVDVRCRATVGEVGNAEQSNINWGKAGRMRWKGKR
                     PTVRGVVMNPVDHPHGGGEGRTSGGRHPVSPWGKPEGRTRRPGKPSDKLIVRRRRTGK
                     KR"
     gene            6532..6813
                     /gene="rpsS"
                     /locus_tag="PL014_09575"
     CDS             6532..6813
                     /gene="rpsS"
                     /locus_tag="PL014_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOT57382.1"
                     /translation="MPRSLKKGPFIDDHLQKKVDVQNEAGTKNVIKTWSRRSVISPDM
                     LGHTLAVHDGRKHVPVFVTESMVGHKLGEFAPTRTFKGHVKDDKKGRRR"
     gene            6813..7211
                     /gene="rplV"
                     /locus_tag="PL014_09580"
     CDS             6813..7211
                     /gene="rplV"
                     /locus_tag="PL014_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOT57383.1"
                     /translation="MATETTVTEARAVARHVRVTPQKARRVIDLIRGKQATDAVSVLK
                     FAPQGAAEPILKVLESAIANARFKADAAAVRFDERDLIIASAFIDEGPTMKRFRPRAQ
                     GRAGRINKRTSHITVVVAPKPAKATNGGNR"
     gene            7211..>7409
                     /locus_tag="PL014_09585"
     CDS             7211..>7409
                     /locus_tag="PL014_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOT57384.1"
                     /translation="MGQKVNPHGFRLGITTEHRSRWFADSTKEGQRYRDYVKEDVAIR
                     KLMSEGMDRAGIARVDIERTRD"
BASE COUNT         1320 a         2611 c         2434 g         1044 t
ORIGIN      
        1 ctcgatggag ttcgactcct acgccgaggt ccccaagtcg gtggccgagg agatcatcaa
       61 gaagacccgg ggcgagtgac cgctagactg gtcgctggtc tcggctgaga agccatccca
      121 accaatccca agtacgacgc cacgtcctgc ccggtcgtat cggcgttcaa ctcactcaag
      181 tcctgaggag gacatcaagt ggcgaaggca aagttcgagc ggaccaagcc gcacgtcaac
      241 atcggcacca tcggtcacat cgaccacggt aagacgacgc tgacggctgc gatttccaag
      301 gttctgcacg acaagtaccc ggacctgaac ccccagttcg cgttcgacga catcgacaag
      361 gcgcccgagg agaagcagcg cggtatcacg atctccatcg cgcacatcga gtaccagacc
      421 gagggtcgtc actacgcgca cgtcgactgc cccggtcacg ctgactacgt gaagaacatg
      481 atcaccggtg cggcccagat ggacggtgcg attctcgtcg tcgccgccac cgacggcccg
      541 atgccgcaga ccaaggagca cgtcctcctg gcccgccagg tcggcgttcc ctacatcgtc
      601 gtggcgctca acaaggccga catggtcgac gacgaggaga tcctcgagct cgtcgagatg
      661 gaggtccgcg agctgctgtc cacctacgag ttcccgggcg acgacctgcc ggtcgtcaag
      721 gtctcggcgc tcaaggccct cgagggcgac ccggagtggg gcgccaccgt gctcgagctc
      781 atggacgccg tcgacgacta catccccgag ccggttcgtg agacggacaa gccgttcctc
      841 atgcccgtcg aggacgtctt cacgatcacc ggtcgtggca ccgtcgtcac cggtcgtatc
      901 gagcgcggca ttctcaaggt caacgaggag gtcgagatcg tcggcatcca cgagggcccg
      961 gccaccaaga ccgttgccac cggcatcgag atgttccgca agctcctcga cgagggtcgc
     1021 gcgggcgaga acgtcggcct gctccttcgc ggcatcaagc gcgaggacgt cgagcgtggc
     1081 caggtcgtcg tcaagccggg ttcgatcacc ccgcacaccg gcttcgacgc caacgtctac
     1141 atcctgtcca aggacgaggg tggccgtcac acgccgttct acgacaacta ccgcccgcag
     1201 ttctacttcc gcaccacgga cgtcaccggt gtcgtgaagc tgcctgaggg caccgagatg
     1261 gtcatgcccg gtgacaacac cgagatggcc gtcgagctca tccagccgat cgcgatggag
     1321 gaaggcctca agttcgcgat ccgcgagggc ggccgcaccg tcggcgccgg ccgcgtcacc
     1381 aagatcacca agtgatctga gggggttcgc cccccgcctg tagcctgagc aacgcaaggg
     1441 ccccgcaccg gaaacggtgc ggggccttcg ctgtgcggtc ccggcggggg tcagtcgagt
     1501 gagcgcaaca tgccgtggcg caacgatcgc cacggcatgt tgcgctcact cgttcacagg
     1561 tccgtctgga ggagggcgct ggcaagcctt cctgcctcac gacaaacgaa gaacatccag
     1621 cacgtcgccg actgctcggg ggttgccggc gatgtgccag gtgtgtccgc ggtgccggac
     1681 caccgccgtt cgtcctccgg ctgcatccac gagcgccctg cccggtagcc agtcccattc
     1741 ggccgtggag tgttggaccc acaggtccat tcggccgctc gcgaccgagg cgaggtcgca
     1801 cgatccggac ccgaacatgc gggccgtggc tgcatctccg acgatcgcga accacggctc
     1861 cgcgaggttg gggtcgcgca gcgtcgcggg atgcaggtag gtggccagca cgcactcgtc
     1921 gagccgtcgg gggaccaatg ccgcaaccgg caccccgtta cgagtcgtcg gcgcaccggc
     1981 accgcccaac cagacgtcgg cggtcgacgg ctggtagacg gctccgagca gggcgccgtc
     2041 cgagccttcg agggcgagcg cgctgcacca gtaggggatc cccatggtga agttgtgggt
     2101 gccgtcaacc gggtcgatca cccatcgacg accggaggtg ccacgcaagg acgcaccctc
     2161 ttcgcccagg actccatcgg acggtcgctc ctgctgcgtc accgagacga cgagggcctc
     2221 gcaggcgcga tctgcagcag tgaccacgtc ggacaacgac gacttgtact cggtgtccac
     2281 cccagcggcg tgcatctgct gggccaggtc cccgcactcg gtgacgagcc gcaaggccag
     2341 agcgtggtct gtggcaagga ctgcgccttc gggcgtctcc gacatggcgc aactgtagga
     2401 gccgtcgtcg ttcggcccgc cgagtgaccc gtagcccaga cccggactgt cgcggggtac
     2461 gccagtccgg gcctgaggtc cagcagccag ggtcagcggg ggccggggcc accctggagg
     2521 acgccggcgt cgaacgcctt gagcaccgag gccttgtcgg atgccgtgag gtcacccgcc
     2581 gtgaccgcag cgtccaggcg cgcggtcagc tcggtcttgt gctcggcctc gtgggcggcc
     2641 ttgaccttct ccagcgccgc cgtgaccttg gcctcgtcga tgccgagctc cttggccaga
     2701 gcggtggcga acgccttctg gcgcgccgtg cgctcggcct cggtgggcgg cgtccgggtc
     2761 tcgccctgcg ccggtgcgtc ggggcgggtg gcctcacgag cggcgctgac cgccgcggtg
     2821 accttggcct cgtcgagcgc gagggcgctg gcgagggcgg cgctgtccat gccgcgcccg
     2881 cccttctggc cgccgcgggg gcccgtgtcg tccgtgctgg tcgaggcggc gccctggttt
     2941 gccgcagcgg caccggagct cgcggtggac gagctggtgc tgctcgggct gggggtggtc
     3001 tcggccgagg cggtggcgac accgactccg gcgccgatgg cgatcaggcc gctcacggcg
     3061 gcgatcttca gggaggtggg ggagacgggc atgcgatctg ctccttgggt tcgtccggtg
     3121 ggcgggatgc ccgccggttc gtgtcccacg gtgtgtggca gggctggcag cgacgtcagc
     3181 gcgacctgtc ccctccatgt gagccggtac cggcacgacg cgccatcagc gcgaaggtgc
     3241 gcgggcggca tccagtcggt ggtgtatcgg ccgatttggt ccggccgcat cccctctggc
     3301 acacttatca ggttgcttgg cgtgcgaacg cgtcccgaga gggtcacgct cgcccaccgc
     3361 cgcgaggtga cgcaccgccg atctcggcgg ggcggctcgt gagcgaagtg ccaggccccg
     3421 gttccaccgg gacagccgaa cctccggaca catgcgggaa accgcctgcg ccgggctgcg
     3481 gagcaagggc gacacgcccg accgcggggg tcggaggttc accggaaccg cactgagaca
     3541 gatgtaggcg agagagagac gggaacgtca gatggcggga cagaagatcc gcatccgctt
     3601 gaagtcgtat gaccacgagg tcatcgacag ctcggcgcgc aagatcgtgg acacggtcac
     3661 ccgcgccgga gcgaccgtgg tcggccccgt gccgctcccg acggagaaga acgtgttctg
     3721 cgtcatccgg tcgccgcaca agtacaagga cagccgcgag cacttcgaga tgcgcaccca
     3781 caagcgcctc atcgacatca tcgacccgac gcccaaggcc gtcgactcgc tgatgcgtct
     3841 cgacctcccg gctgacgtca acatcgagat caagctctga ggctgacgac atgaccactt
     3901 ccaccaagac ccagaagggt ctgctgggca ccaagctcgg catgacccag gtgtgggacg
     3961 agaacaaccg cctcgtgccc gtcaccgtga tccaggccgg cccgaacgtc gtgacccagg
     4021 tccgcgacgc cgagaccgac ggctacgcgg ccgttcagct cggctacggc gcgatcgacc
     4081 cgcgcaaggt caccaagccg ctcaccggcc acttcgagaa ggctggggtc acccctcgcc
     4141 gtcacgtcgt cgagctgcgc accgctgacg cgggcgacta cagcctcggc caggagctcg
     4201 gcgccgacac cttcgaggtc ggccagagcg tcgacgtcac cggcacgacc aagggcaagg
     4261 gcttcgcggg cgtcatgaag cgtcacggct tcaagggcgt ctcgtcgtcg cacggttcgc
     4321 accgcaacca ccgcaagccc ggctcgatcg gtggggcctc cacgcccggc cgcgtcttca
     4381 agggcatgcg catggccggc cgcatgggcg gcgtgcgcca gaccacccag aacatcaccg
     4441 tccacgccgt ggacgccgag aagggcctgc tgctgctcaa gggtgccgtc cccggccccc
     4501 gcggcgccgt cgttctcgtg cgctcggccg cgaagggggc gtgacccgca tggcaaccaa
     4561 gacgctcacc gtcgacctcc ccgcggagat cttcgacgtg cagaccaacg tcccgctcat
     4621 ccaccaggtc gtcgtcggcc agctcgcggc agctcgtcag ggcacgcact cgacgaagac
     4681 ccgggcggac gtgcgcggtg gtggccgcaa gccctacaag cagaagggca ccggccgcgc
     4741 ccgtcagggc tcgacccgcg caccgcagtt cgccggtggt ggcatcgtcc acggcccgca
     4801 gccgcgtgac tactcgcagc gcacccccaa gaagatgaag gccgccgccc tgcgcggggc
     4861 cctgtcggac cgggcgcgcc acgaccgcat ccacgtcgtc gagtcgatct ccaacggaga
     4921 cgtcccctcg gccaaggctg ccgtccagct gctcgccggc ctgacgctgc gcaagaacgt
     4981 gctcgtcgtg ctcgaccgca gcgacatcgt gtcgctgaag tcggtgcgca acatcgccga
     5041 cctgcacgtg ctcgccgtcg accagctcaa cacctacgac gtgctgtgcg ccgatgacgt
     5101 cgtgttcacc aaggcggcgt tcgacgctct cgtcgaccgc ttccagggca ccacggcgtc
     5161 cgcgatccag gacaccacgc cggccgaggc cgtcgaggtc caggacgaca agccggccaa
     5221 gaccaagaag gccgacaagg ccgaggcggt ccaggacgac aagccggcca aggccaagaa
     5281 ggccgacaag gccgacaagc ccgcgaaggc cgacaagccc gcgaaggcca agaaggccga
     5341 caaggctgat gtggaggacg acaagtgagc cagggcaaga acccccgcga catcctgatc
     5401 gcgccggtgg tgtccgagaa gtcctacggg ctgctcgacc agggcaagta caccttcatc
     5461 gtcgacccgc ggtcgaacaa gaccgagatc aagatcgccg tcgagcagat cttcaacgtg
     5521 aaggtcgact cggtccacac catgaaccgc gtgggcaagg cccgtcgtac gcggttcggc
     5581 atcggcaagc gcaaggacac caagcgcgcg atcgtcaccc tccgcgaggg ctcgatcgac
     5641 atcttcggca gcgccggcgc ctgaggaagc tgaggacaaa ccatgggtat ccgtaagtac
     5701 aagccgacga cgccggggcg tcgcgggtcg agcgtggccg acttcgtcga ggtcacccgc
     5761 tccacccccg agaagtccct cgtccgtccg ctcaccaaga ccggtggccg caacaacaac
     5821 ggccgcatca ccacccgtca catcggtggt ggccacaagc gtgcctaccg cgtcatcgac
     5881 ttccgtcgcc acgacaagga cggcatcccg gcgaaggtcg ctcacatcga gtacgacccg
     5941 aaccgcaccg cgcggatcgc gctcctgcac ttcgccgatg gcgagaagcg ctacatcctg
     6001 gcccccaagg gcctggagca gggcatgcag atcgagcacg ggccgagcgc cgacatcaag
     6061 cccggcaaca acctgccgct gcgcaacatc ccgaccggta cggtcatcca cgccgtcgag
     6121 ctgaagcccg gtggcggcgc gaagatcgcc cgttcggccg gtgtgcgcgt gcagctcgtc
     6181 gccaaggacg gcccctacgc gcagctgcgc atgccgtccg gtgagatccg caacgtcgac
     6241 gtgcgctgcc gcgccaccgt cggcgaggtc ggcaacgccg agcagtcgaa catcaactgg
     6301 ggcaaggccg gccgcatgcg ctggaagggc aagcgcccga ccgtccgcgg tgtcgtgatg
     6361 aaccccgtcg accacccgca cggtggtggc gagggccgca cgtccggtgg tcgtcacccc
     6421 gtgtccccct ggggcaagcc cgagggtcgc acccgccgcc ccggcaagcc cagcgacaag
     6481 ctcatcgttc gtcgccgccg tactggcaag aagcgctgat aggagcctgg aatgcctcgt
     6541 agcctgaaaa agggcccctt catcgatgac caccttcaga agaaggtgga tgtccagaac
     6601 gaagcgggca ccaagaacgt catcaagacc tggtcgcgcc ggtcggtcat ctcgcccgac
     6661 atgctcggcc acaccctcgc cgtccacgac ggccgcaagc acgtcccggt gttcgtcacc
     6721 gagtcgatgg tcggccacaa gctcggcgag ttcgcaccga cgcgcacctt caagggtcac
     6781 gtcaaggacg acaagaaggg gcgtcgtcgc tgatggccac cgaaaccact gtcaccgagg
     6841 ctcgcgctgt cgcgcgccac gtccgggtga ccccgcagaa ggcccgtcgg gtcatcgacc
     6901 tcatccgcgg caagcaggcc acggatgccg tgtcggtcct gaagttcgcg ccccaaggtg
     6961 cggccgagcc gatcctgaag gtcctcgagt ccgcgatcgc caacgcccgg ttcaaggctg
     7021 acgccgccgc cgtgcgcttc gacgagcgtg acctcatcat cgcctccgcg ttcatcgacg
     7081 aggggcccac catgaagcgt ttccgtccgc gggcccaggg ccgcgccggt cgcatcaaca
     7141 agcgcacgag ccacatcacc gtggtcgtcg ctcccaagcc cgccaaggcc acgaacggag
     7201 gcaatcgctg atgggacaga aggtcaaccc gcacggcttc cgtctgggca tcaccaccga
     7261 gcaccgcagc cgctggttcg ccgactccac caaggagggt cagcgctacc gcgactacgt
     7321 caaggaggac gtggcgatcc gcaagctcat gtctgagggc atggaccgcg ccggcatcgc
     7381 ccgcgtcgac atcgagcgca cccgtgacc
//
LOCUS       DAOSOC010000125         7404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_10036_length_7404_cov_9.621173, whole genome shotgun sequence.
ACCESSION   DAOSOC010000125 DAOSOC010000000
VERSION     DAOSOC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10036_length_7404_cov_9.621173"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PL014_09590"
     CDS             <1..838
                     /locus_tag="PL014_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOT57385.1"
                     /translation="KDFGEERVIDTPLAEAGILGTAIGMALRGYRPVIEIQFDGFVYP
                     AFDHIVSQVAKLHARSLGYVKMPLVIRIPFGGGIGAVEHHSESNEAYFAHTAGLRVVA
                     VSNPHDAHWMIQQAIETDDPVVFYEPKRRYWDKGEVGDGPSGSLGSARVLREGTDVTL
                     LAYGPMVKTCLQAAEAAAEEGRNLEVIDLRSLSPLDEGTILASARKTGRVVVVHEAST
                     FLGMGSEISALITERAFHHLEAPVLRVGGYNVPYPPSRIEEEFLPDLDRVLDAVDRSL
                     AY"
     gene            854..2305
                     /locus_tag="PL014_09595"
     CDS             854..2305
                     /locus_tag="PL014_09595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOT57386.1"
                     /translation="MALREFKLPDPGEGLTEADLVTWHVAVGDTVKVNDIVVEVETAK
                     SLVELPVPFAGTVAALHAAEGDTVDVGSVIITVDDGVGGSSDAAAPAPAAAAPPAGAP
                     ASDAGAPAGGSGEEIAEGLIGGTTSTGRTAVLVGYGVKQTEAKRRPRTGGGAPTVPAS
                     TNRSPQVQPLSGERTRTLAKPPVRKLAKDLGLDLAAITPTGEGGIVTRADVEAAAARA
                     TGTAASAQEISDAVPAASRSGRPTASGDREERIPVKGVRKMTAQAMVASAFTAPHVTE
                     WVTVDVTATMDLVERLKADREFRDVKVTPLLVLAKAMCVAVRRNPGINASWDEAAQEI
                     VVKHYVNLGIAAATPRGLLVPNIKDADAMTMRELADAIGTLTATAREGRTQPADMSGG
                     TITITNVGVFGIDAGTPILNPGESAILAFGTVSRRPWVVTAADGSERIEPRWVTTLAL
                     SFDHRLVDGELGSRYLADVAALLGDPGRALVWG"
     gene            2373..2822
                     /locus_tag="PL014_09600"
     CDS             2373..2822
                     /locus_tag="PL014_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOT57387.1"
                     /translation="MEANRLVEARPLGLVLLGGAIGTAGRERLHLLLPAEGGIPWSVF
                     LVNIVGAALLGALLAHLAAVEPETPRRREVRLFVGTGILGGFTTYSALATDTVLLAGS
                     RLGLAMTYAVASVVLGVLAAGIGFRVGRLSADRRADRRADPARGGRA"
     gene            2819..3196
                     /locus_tag="PL014_09605"
     CDS             2819..3196
                     /locus_tag="PL014_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HOT57388.1"
                     /translation="MSVPVFLLGCLGGGIGACLRYLGDGLVMRRATPGFPSGIFLVNA
                     LGSFLLGLLVGSFEAGLVDGTWLFVLGGGVLGGFTTFSTAMVDTVRLMHQEAWGRAAA
                     NAVGMLVVGVVLALLGLTLGRGL"
     gene            3272..4735
                     /locus_tag="PL014_09610"
     CDS             3272..4735
                     /locus_tag="PL014_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57389.1"
                     /translation="MNDPTGVVFPEVDGRRSTSATGRGIVADALRPVDPVGAAAAQRE
                     TNWRSGYLTHFRRLVEAGLTSPQDAVTIADAGLASVHDRMRWRDPDGTECALDAALGG
                     VVGSPADNLVTQSHGGGGEPDTALTVPYRGTRLAGDDLRRRLDTWVDDGVVEPSLREA
                     VGLVLDNPDWLRLEGRTVVVLGAAAEMGPLRSLLRWGATVAAVDLPRPDLWTRVLDDT
                     ASSAGRLLVPASPGTSPLAERAGVDLIGGLPTATGWVCGLPGQLVVGNYVYADGATNV
                     RVSTAVDAMTRIVRLARPDTALSFLATPTDVFAVPGEAVDASAQSYAQRRTSKVLRGP
                     LRTLSGGRLLRRNYQPGEDPGINDSVVLQQGPNYLLAKRIQRWRATSARARGATVSFK
                     IAPPTRTRSVVKNRALAAAYAGSHRFGVEVFEPGTANTLMAALLVHDLTVGSPRQEHP
                     WQDEAYAAAHGGLWRAAYDPRSALGLAALLGAASARP"
     gene            4748..5950
                     /locus_tag="PL014_09615"
     CDS             4748..5950
                     /locus_tag="PL014_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT57390.1"
                     /translation="MSASSSMPKRPSDPLDLIDLDSQLSDEEKAIRAATRQVCADVVD
                     PHVMQWYEDGDLPARDLALEFGKVGLLGMHLEGYGCAGMSAVDYGLACLELEASDSGI
                     RSLVSVQGSLAMFAIWRFGTEEHKQQWLPRMAAGEAIGCFGLTEPDHGSDPASMRTRA
                     RKDGDDWVLNGSKMWITNGSIADVAVVWANAGEEHGGIRGFVVPTDTPGFAAHKITHK
                     MSLRASVTSELTFENLRLPADAVFPDVTGLKGPLACLSEARYGIVWGALGAARSSLDA
                     ARRYSMERIQFGRPIAGFQLTQAKLANMTTELQLGQLLALHLGRRKDTVGLRPEQVSV
                     GKYNNITKAIEICRTARTILGANGVSGEYPVLRHANNLESVLTYEGTVEMHTLVIGQA
                     LTGESAFR"
     gene            complement(6063..6659)
                     /locus_tag="PL014_09620"
     CDS             complement(6063..6659)
                     /locus_tag="PL014_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57391.1"
                     /translation="MGDGSMTDAVTRRGVARGAAWSVPLVAAVAAAPQAAATVCPGAS
                     FLGVIFSATQDQISVTNSGPVPWPAGTTITWVIQNVRQPAGSSAFAVGPVSGLTVGTV
                     PASIARNASATVTFSRTTALAVGASVSWRFSLAEYRYNSRVTVSFVGTTLANCPPLTA
                     CISNRFSEIGSTCPTGGAAARVAAADANATPDPIVPRP"
     gene            6788..>7404
                     /locus_tag="PL014_09625"
     CDS             6788..>7404
                     /locus_tag="PL014_09625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT57392.1"
                     /translation="MLSAYAPLFRVPGALRFIAGTALSRTGGSMFGVAVIVMVSERRG
                     SFGVAGAVSAVGVAVLAVAGPVIGRLIDKHGQRRVALPFILLSSCAGALTAYFSWSNA
                     PVWTLFVFYGLSAVLPEPGPMSRARWAHIFRDDPDQLHTAMSFEQVADEASFVLGPVV
                     AVLVSTLWFPEAGLILAEILFTAGMLIFLAARATEPPIVPHADRPG"
BASE COUNT          985 a         2762 c         2579 g         1078 t
ORIGIN      
        1 gaaggacttc ggcgaggagc gggtcatcga caccccgctg gccgaggccg gcatcctcgg
       61 caccgccatc ggcatggccc tgcgcggcta ccgcccggtc atcgagatcc agttcgacgg
      121 gttcgtctac ccggcgttcg accacatcgt cagccaggtc gcgaagctgc acgcccgctc
      181 gctggggtac gtgaagatgc cgctcgtcat ccgcatcccg ttcggcggtg gcatcggcgc
      241 ggtcgagcac cacagcgagt ccaacgaggc ctacttcgcc cacacggcgg ggctgcgcgt
      301 cgtcgccgtc tccaacccgc acgacgccca ctggatgatc cagcaggcca tcgagaccga
      361 cgaccccgtc gtgttctacg aacccaagcg ccgctactgg gacaagggtg aggtcggcga
      421 cgggccctcc gggtcgctcg gctcggcgcg cgtgctgcgc gagggcaccg acgtcacgct
      481 gctggcctac ggccccatgg tcaagacgtg cctccaggcc gccgaggccg cggccgaaga
      541 ggggcgcaac ctcgaggtca tcgacctgcg ctcgctctcg ccgctcgacg agggcacgat
      601 cctggcctcg gcccgcaaga ccggccgggt cgtcgtcgtc cacgaggcat cgacgttcct
      661 cggcatgggg tcggagatct cggcgctcat caccgagcgg gccttccacc acctcgaggc
      721 cccggtcctg cgcgtcggcg gctacaacgt gccctacccg cccagccgca tcgaggaaga
      781 gttcctcccc gacctcgacc gcgtgctcga cgccgtcgac cgctccctcg cgtactgaaa
      841 ggggttctcg cagatggctc ttcgtgagtt caagctcccc gacccgggtg agggtctgac
      901 cgaggccgac ctcgtcacgt ggcacgtggc cgtcggtgac acggtcaagg tcaacgacat
      961 cgtcgtcgag gtcgagacgg ccaagtcgct cgtcgagctt ccggtgccct tcgccggcac
     1021 cgtcgcggcc ctgcacgccg ccgagggcga caccgtcgac gtcggttcgg tcatcatcac
     1081 cgtcgacgac ggggtgggcg gttcctcgga tgccgccgcg ccggctcccg ctgccgccgc
     1141 accgcccgct ggtgccccgg catccgacgc gggtgccccg gctggtggct cgggcgagga
     1201 gatcgccgag ggcctcatcg gtggcacgac ctcgacggga cgcaccgcgg tgctcgtcgg
     1261 ctacggggtc aagcagaccg aggccaagcg ccgcccgcgc accggcgggg gagcaccgac
     1321 cgtccccgca tcgaccaata ggtcaccaca ggtacagccg ctgtccgggg agcgcacccg
     1381 caccctggcc aagccgccgg tgcgcaagct cgccaaggac ctcgggctcg acctcgccgc
     1441 catcaccccg accggcgagg gtggcatcgt cacccgcgcc gacgtcgagg cggcggccgc
     1501 gcgcgcgacg ggtaccgcgg catccgcaca ggagatctcg gatgccgtac ccgccgcgtc
     1561 ccgctcgggc cgccccacgg catccggcga ccgggaggag cggatccccg tcaagggcgt
     1621 gcgcaagatg acggcgcagg ccatggtcgc gtcggccttc accgcgcccc acgtcaccga
     1681 gtgggtgacc gtcgacgtca ccgcgacgat ggacctcgtc gagcggctca aggccgaccg
     1741 cgagttccgc gacgtcaagg tcactccgct gctcgtgctc gcgaaggcga tgtgtgtcgc
     1801 ggtgcgccgc aaccccggca tcaacgccag ctgggacgag gccgcccagg agatcgtcgt
     1861 caagcactac gtcaacctcg gcatcgccgc ggcgaccccg cgcgggctcc tcgtgccgaa
     1921 catcaaggat gccgacgcga tgacgatgcg tgagctcgcc gacgcgatcg gaaccctcac
     1981 ggccaccgcc cgcgaggggc gcacccagcc cgccgacatg agcggcggga cgatcacgat
     2041 caccaacgtc ggggtgttcg gcatcgacgc cgggacgccg atcctcaacc ccggtgagtc
     2101 cgcgatcctc gcgttcggca cggtgtcgcg tcgcccgtgg gtcgtcaccg cggccgatgg
     2161 cagcgaacgc atcgagccgc gctgggtgac cacgctggcg ctgagcttcg accaccgact
     2221 cgtcgacggc gagctcggca gccgctacct cgccgacgtc gccgccctgc tcggcgaccc
     2281 ggggcgggcc ctggtctggg gctgaacgcc cgccgcctcc gtcgcctccg tcgcctccac
     2341 cacggggggg cgccctcact agggtgtgcc ccgtggaggc gaatcggctg gtcgaggctc
     2401 ggccgctggg cctggtgctg ctcggcggcg ccatcggaac cgccggtcgg gagcgactgc
     2461 acctcctcct gcctgccgag ggcggcatcc cgtggtcggt gttcctcgtc aacatcgtcg
     2521 gcgccgccct gctcggtgcc ctcctcgccc acctcgccgc cgtggaaccg gagacgcccc
     2581 ggcgacgtga ggtgcggctg ttcgtgggca ccggcatcct cggcggtttc accacctaca
     2641 gcgccctggc gacggacacc gtgctgctgg ccggctcccg cctgggtctg gcgatgacgt
     2701 atgccgtggc gagcgtcgtc ctcggcgtcc tcgccgcggg gatcggcttt cgtgtcgggc
     2761 ggctgagtgc cgaccggcgt gctgaccggc gtgccgaccc ggcgcgggga ggtcgggcat
     2821 gagcgtcccc gtgttcctcc tcggctgcct cggcgggggc atcggcgcct gcctgcgcta
     2881 cctcggcgac gggctggtca tgcggcgtgc cacaccgggc tttccgtcag gcatcttcct
     2941 cgtcaacgcc ctcggatcgt tcctgctcgg cctgctcgtc gggtcgttcg aggcggggct
     3001 ggtcgacggc acgtggctgt tcgtcctcgg gggcggcgtg ctcgggggct tcacgacgtt
     3061 cagcacggcg atggtcgaca ccgttcgact catgcaccag gaggcctggg gtcgggctgc
     3121 tgccaacgcg gtgggcatgc tcgtggtggg cgtggtgctg gccctgctcg gcctgaccct
     3181 cggccgcggg ctctgacggc ggcgctcgcc gccctggtcc tcgcgcacgt ggcagagtgg
     3241 ccagcgttgc ccgaacgtcg tcaggagtga catgaacgac cccaccggtg tggtcttccc
     3301 cgaggtcgac ggccgccgct cgaccagcgc gacaggtcgc ggcatcgtgg ccgacgcgct
     3361 gcgccccgtc gaccccgtcg gagcggccgc cgcccagcgc gagacgaact ggcgcagcgg
     3421 ctacctcacc cacttccggc gcctggtgga ggcgggcctg accagcccgc aggatgccgt
     3481 gaccatcgcg gatgccggcc tggcctcggt gcacgaccgg atgcggtggc gtgacccgga
     3541 cggcacggag tgcgccctgg atgccgcgct gggcggggtc gtcggcagcc ccgccgacaa
     3601 cctcgtgacg cagagccacg gtggcggtgg tgagcccgac accgccctga cggtgcccta
     3661 ccggggcacc cggctcgccg gggacgacct gcgccgccgg ctggacacgt gggtggacga
     3721 cggggtcgtc gagccgagcc tgcgcgaggc cgtcgggttg gtgctcgaca accccgactg
     3781 gctgcggctc gaggggcgca ccgtcgtcgt gctcggggcg gcggccgaga tggggccgct
     3841 gcgctcgctg ctgcgctggg gtgccaccgt cgccgcggtc gacctgcccc gcccggacct
     3901 gtggacccgc gtcctcgacg acacggcatc cagcgccggc cggctgctcg tgccggcctc
     3961 cccgggcacg tccccgctgg ccgaacgggc cggggtcgat ctcatcgggg gtctgccgac
     4021 cgcgaccggc tgggtctgcg ggctgcccgg gcagctcgtc gtcggcaact acgtctacgc
     4081 cgacggggcg acgaacgtgc gggtgagcac cgcggtcgac gccatgaccc ggatcgtgcg
     4141 cctggcccgg ccggacaccg ccctgtcgtt cctcgcgacc ccgaccgacg tgttcgccgt
     4201 gccgggggag gccgtcgatg cctcggcgca gtcctatgcg cagcgccgca cctccaaggt
     4261 gctgcgcggc ccgctgcgca ccctgtcggg cgggcggctg ctgcgccgca actaccaacc
     4321 cggtgaggac cccggcatca acgactccgt ggtgctccag cagggcccga actacctgct
     4381 cgccaagcgg atccagcgct ggcgggccac gtcggcccgg gcgcgggggg ccacggtctc
     4441 gttcaagatc gccccgccca cccgcacccg ctcggtcgtg aagaaccggg cgctggctgc
     4501 cgcgtatgcc gggtcgcacc gcttcggggt cgaggtcttc gagccgggca cggccaacac
     4561 cctcatggcg gctctgctcg tgcacgacct caccgtcggc tcgccccgcc aggagcaccc
     4621 gtggcaggac gaggcgtatg ccgcggcgca cggcgggttg tggcgtgccg cgtacgaccc
     4681 gcgcagtgcg ctcgggttgg ccgccctgct cggcgccgcc tccgcccgcc cctgagagga
     4741 tgtcggtatg agtgcctcat cgtcgatgcc gaagcgcccc tccgacccgc tcgacctcat
     4801 cgacctcgac agccagctga gcgacgagga gaaggcgatc cgggcagcca cccgccaggt
     4861 gtgcgccgac gtcgtcgacc cgcacgtcat gcagtggtac gaggacggcg acctgccggc
     4921 ccgcgacctc gcgctggagt tcggcaaggt cgggctgctc ggcatgcacc tcgaggggta
     4981 cggctgcgcc ggcatgtcgg ccgtcgacta cggcctggcc tgcctggagc tcgaggcgag
     5041 cgactccggc atccggtccc tggtgtcggt gcagggcagc ctcgcgatgt tcgcgatctg
     5101 gcgcttcggc accgaggagc acaagcagca gtggctgccg cggatggccg ccggtgaggc
     5161 gatcggctgc ttcggcctga ccgagcccga ccacggctcc gaccccgcct cgatgcgcac
     5221 ccgcgcgcgc aaggacggag acgactgggt gctgaacggc tcgaagatgt ggatcaccaa
     5281 cggctcgatc gccgacgtcg ccgtggtctg ggcgaacgcg ggcgaggagc acggcggcat
     5341 ccgcggcttc gtggtgccca ccgacacacc gggattcgcg gcgcacaaga tcacccacaa
     5401 gatgtcgctg cgggcctcgg tgacctcgga gctcaccttc gagaacctgc gcctccccgc
     5461 agatgccgtg ttccccgacg tcacggggtt gaagggcccg ctcgcgtgcc tgtcggaggc
     5521 acgctacggc atcgtctggg gagcgctcgg cgcggcccgc tcgtcgctgg atgccgcgcg
     5581 gcgctactcg atggagcgca tccagttcgg ccggccgatc gccgggttcc agctcaccca
     5641 ggccaagctc gcgaacatga ccaccgagct gcagctcggg cagctgctcg cgctgcacct
     5701 cgggcgacgc aaggacaccg tgggcctgcg ccccgagcag gtctcggtcg gcaagtacaa
     5761 caacatcacc aaggccatcg agatctgccg cacggcccgc acgatcctcg gcgccaacgg
     5821 ggtgagcggg gagtacccgg tgctgcggca cgccaacaac ctcgagtcgg tgctgaccta
     5881 cgagggcacc gtcgagatgc acacgctcgt catcggccag gccctcacgg gggagtcggc
     5941 cttccgctga cgcgcgacgt gcgcccgcgt gctgggcgcc agtgccccga ccgacgcaga
     6001 agttcagttc tcgaccggta attgctgcgt gcgatccgag gttctgcgtt cgggctgact
     6061 gctcagggcc gcggcacgat cgggtccggt gtggcgttgg cgtcggccgc tgcgacccgc
     6121 gccgccgcac ccccggtcgg gcaggtcgag ccgatctcgg agaaccggtt ggagatgcag
     6181 gccgtcagtg gcgggcagtt cgccagcgtg gtgccgacga agctcacggt gacccgagag
     6241 ttgtagcggt actcggccaa ggagaaccgc cacgagacgc tggcccccac ggcgagtgcg
     6301 gtcgtgcggc tgaacgtcac cgttgcagag gcgtttcggg cgatcgacgc ggggaccgtg
     6361 cccaccgtga gcccgctgac cggcccgacg gcgaacgccg acgaccccgc cggttgccgg
     6421 acgttctgga tcacccacgt gatggtcgtg ccggccggcc acggcaccgg cccgctgttc
     6481 gtcacgctga tctggtcctg cgtcgcgctg aagatgacgc cgaggaacga cgcccccggg
     6541 cacaccgtgg ccgcagcctg tggcgctgcc gcgacggccg caaccagcgg gacggaccat
     6601 gccgcccccc gcgccacgcc ccgacgcgtc acggcatccg tcattgaccc atcacccatc
     6661 actcacccgc cactccaaac cccaccggca ccacgaacat ccccgcccca ccgaacagtg
     6721 gctggaacga ccctcaccgt gtgacaggct cctctcaaca gatagccgct cgatcgaggt
     6781 gcctcccgtg ttgtccgcct acgccccgct ctttcgtgtg cccggagccc tgcgcttcat
     6841 cgccggcacc gcgctctcgc gcaccggggg gtcgatgttc ggtgtggccg tcatcgtcat
     6901 ggtcagcgaa cgccggggct ccttcggggt ggccggcgcg gtgtcggccg tgggggtggc
     6961 cgtcctcgcg gtcgccgggc cggtcatcgg gcggctcatc gacaagcacg gccagcgtcg
     7021 cgtggccctg ccgttcatcc tcctgtcgtc gtgcgccgga gccctcacgg cctacttctc
     7081 ctggtccaac gcccccgtgt ggacactctt cgtgttctac ggcctgagcg ccgtgctgcc
     7141 cgagccgggg ccgatgtcgc gggcgcgctg ggcccacatc ttccgcgacg acccagacca
     7201 gctgcacacg gcgatgtcct tcgagcaggt ggctgacgag gcgtccttcg tgctgggccc
     7261 ggtggtggcc gtgctcgtgt cgaccctgtg gttccccgag gccggcctca tcctggccga
     7321 gatcctcttc accgcgggca tgctcatctt ccttgcggcg cgggccaccg agccgccgat
     7381 cgtgccgcac gccgaccgcc ccgg
//
LOCUS       DAOSOC010000126         7403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_10040_length_7403_cov_8.212575, whole genome shotgun sequence.
ACCESSION   DAOSOC010000126 DAOSOC010000000
VERSION     DAOSOC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10040_length_7403_cov_8.212575"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /gene="whiA"
                     /locus_tag="PL014_09630"
     CDS             <1..258
                     /gene="whiA"
                     /locus_tag="PL014_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOT57393.1"
                     /translation="AGARVERALEILGVDIPDHLREAGELRLAHKEASLEELGQLAKP
                     PMTKDAVAGRIRRLLAMADKRAADDGIPNTEAGLTPDMLDA"
     gene            400..1404
                     /gene="gap"
                     /locus_tag="PL014_09635"
     CDS             400..1404
                     /gene="gap"
                     /locus_tag="PL014_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT57394.1"
                     /translation="MTVRVGINGFGRIGRNFFRAVQASGADIEIVAANDLMDNKAIAH
                     LLKYDSVLGVLADDVSSTEDSITVGDKTIKIFAEKNPADIAWGDVGADIVIESTGFFT
                     DATKAHAHIDGGAKKVIISAPASNEDATFVMGVNHQDYDPANHHVISNASCTTNCLAP
                     MAKPLSDELGIVKGLMTTVHAYTSDQNLLDGPHRDMRRARAAALSIIPTSTGAAKAIG
                     LVMPELKGKLDGYALRVPTPTGSATDLTFEAGRETTVEEVNAIVKAAAEGPLKGFLKY
                     TEDPIVSADIVTDPSSCIFDSGLTKVIGTQVKVVGWYDNEWGYSNRLIDLTLLVGKSL
                     "
     gene            1426..2631
                     /locus_tag="PL014_09640"
     CDS             1426..2631
                     /locus_tag="PL014_09640"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT57395.1"
                     /translation="MGDLSSLGDVRGKRVLVRSDLNVPLDGTTITDDGRIRASVPTIS
                     ALAEAGARVIVCAHLGRPKGAPEDKYSLAPVVGRLSELLGRPVAFATDTVGESARATV
                     DALGDGDVALLENLRFNAGETAKVDEERAEFADALAALADAYVSDGFGVVHRKQASVY
                     DIATRLPSAMGGLVATEVDVLKRLTVDPERPYAVVLGGAKVADKLAVIDNLMNTADRL
                     LIGGGMLFTFLAAQGHEIGKSLFDADSVDACRDYLRRAEETGVEIVLPVDVICGREFS
                     ADTETVTVAADAIPADMMGLDIGPASAQLYAEKLADTRTVFWNGPMGAFEMAPFAEGT
                     RRLAQALVDVTAQGALTVVGGGDSAAAVRQLGFEDAQFGHISTGGGASLEYLEGKELP
                     GIAILTKEH"
     gene            2635..3432
                     /gene="tpiA"
                     /locus_tag="PL014_09645"
     CDS             2635..3432
                     /gene="tpiA"
                     /locus_tag="PL014_09645"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOT57396.1"
                     /translation="MTSGRTPLLAGNWKMNLDHLQATHLVQKLDWTLKDGKHDFGAVE
                     VAVLPPFTDLRSVQTLIDGDRLELKFGAQDLSVHDAGAYTGEISGAFLAKLGCSYVAV
                     GHSERREYHHEDDSVVNAKVKAAYRHGITPIFCCGEGLEIRQAGTHVEHVLAQVEAGL
                     DGLSVEDARSIVVAYEPIWAIGTGEVATPEDAQEVCAAIRTKLAELYSGDVADGIRVL
                     YGGSVKSSNVAAIMAKEDVDGALVGGASIDPAEFAAICRFRDHLATA"
     gene            3513..3758
                     /gene="secG"
                     /locus_tag="PL014_09650"
     CDS             3513..3758
                     /gene="secG"
                     /locus_tag="PL014_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOT57397.1"
                     /translation="MDAVRIGLQVLLVLGGLFLTLLILMHKGKGGGMSDMFGGGMSSS
                     LGGSSVAERNLDRITIGVALVWFTCVVGLGVLERFAV"
     gene            3773..4123
                     /locus_tag="PL014_09655"
     CDS             3773..4123
                     /locus_tag="PL014_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HOT57398.1"
                     /translation="MAGGNAIRGSRVGAGPMGEAERGEVAPRVHISFWCSNGHETRPS
                     FSQEPGLVIPEQWDCPRCGFPAGRDQDNPPAPMKVEPYKTHLAYVKERRSDVDGEAIL
                     DEALGSLRDRGLIQ"
     gene            complement(4148..4645)
                     /locus_tag="PL014_09660"
     CDS             complement(4148..4645)
                     /locus_tag="PL014_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3806 domain-containing protein"
                     /protein_id="HOT57399.1"
                     /translation="MGLFDRLRGRDADSTPDHAAASADDVTADDSGVEALEEIGIRRL
                     TAQDEAALDRARAGYAAHGIEPSDLQSIAAAYDRAVEADDGAIASADVVTLISTAIGD
                     HLVAAAGHRWVVSTDPFGTDLAVEPPRHGIPVVTRTMVAVRWMGRERGWIPGVVGHLA
                     RASRG"
     gene            complement(4650..5384)
                     /gene="pgl"
                     /locus_tag="PL014_09665"
     CDS             complement(4650..5384)
                     /gene="pgl"
                     /locus_tag="PL014_09665"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOT57400.1"
                     /translation="MTGPTTVVVHPGRQALADAAAARLVVAIVDAQCARGVAQISMTG
                     GSMGSQIISSLCAVPARDAVDWSQVHVWWGDERWLPAGDPDRNDTQNDQAGLATLGLT
                     PEHVHRVAGPDTSESAEASAALYEQALREHGGGMFDVMVLGVGPDGHVASLFPHHPAA
                     ASTAGIAIAVHDSPKPPPTRVSLTRECLERSREVWFLVSGADKADAVLRGTTGAPFET
                     TPAAHVHGIERTLWLVDDAAAGDLEG"
     gene            complement(5381..6517)
                     /locus_tag="PL014_09670"
     CDS             complement(5381..6517)
                     /locus_tag="PL014_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HOT57401.1"
                     /translation="MIVDLPDTTTAAISARLVRLRADTGSMALSRVLTLLVVVDAAHA
                     DAAIETANDASRQHPCRIIVVIAGSKRGAARLDGQIRVGGDAGASEVVVLRLFGRLAS
                     HGRAVVTPLLLADSPIVAWWPANGPHEPSKDPIGMMAQRRVTDAARFSTTSRTVLRRL
                     SDGYAAGDSDLAWSRITLWRGLLTAALDQPPYEPVTSAVVVAAADSPSGDLLAGWLAA
                     RLKCPVSIARARSGSGIVSVRLERDSGSIDLVRPVDGNTATLAQPGQPVRTLALAHRG
                     DAECLADELRRLDPDEVYHDALVVGLRRCTVSRQTASEAVRAGRAPSVAEAERTAKRL
                     RRAARASTRSAMVEAAPMPPKVDDPEVVKQAAKDKLAEVKERRS"
     gene            complement(6514..>7403)
                     /gene="zwf"
                     /locus_tag="PL014_09675"
     CDS             complement(6514..>7403)
                     /gene="zwf"
                     /locus_tag="PL014_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOT57402.1"
                     /translation="FANQMFEPVWNANYVDHVQITMAEDIGIGGRAGYYDGIGAARDV
                     IQNHLLQLLALTAMEEPASFDAHGLRLEKEKVLSAVRIPADLSTGTARGQYSAGWQGG
                     EEVVGYLEESGVAKGSGTETYAAIRLDVATRRWAGVPFYLRTGKRLGKRVTEIAVVFK
                     KAPHLPFTDTATEELGQNAIVIRVQPDEGVTLRFGAKVPGAQMEVRDVTMDFGYGRAF
                     TESSPEAYERLILDVLLGEPPLFPRHEEVELSWQILDPIEKYWATKGRAPEQYPAGTW
                     GPASADAMMQRDGREWRLP"
BASE COUNT         1101 a         2617 c         2573 g         1112 t
ORIGIN      
        1 gccggagccc gggtcgagcg ggccctggag atcctcggtg tcgacatccc cgaccacctg
       61 cgcgaggcgg gggagctgcg tctggcccac aaggaggcct cgctcgagga actcggccag
      121 ctcgcgaagc cgccgatgac caaggacgcc gtcgccggcc gcatccggcg actgctcgcc
      181 atggccgaca agcgagcggc cgacgacggc atcccgaaca ccgaggccgg gctcaccccg
      241 gacatgctcg acgcctgagt cgtctcatgc gcaagcggtt actcgcccgt actccggacg
      301 ccgcgcaacg gcatccgagg tgggtctagt gttagtgacg aaacccctga gtgccgctca
      361 tcggcggcac cgttcgatga gtggaaggca gcacataccg tgaccgttcg cgtagggatc
      421 aacggcttcg gccgcatcgg ccgcaacttc ttccgggctg tccaggcctc cggcgccgac
      481 atcgagatcg tcgccgccaa cgacctgatg gacaacaagg ccatcgcgca cctgctcaag
      541 tacgactcgg tgctcggtgt tctcgccgac gacgtctcct cgaccgagga ctcgatcacg
      601 gtgggcgaca agaccatcaa gatcttcgcc gagaagaacc ccgccgacat cgcctggggt
      661 gacgtgggcg ctgacatcgt catcgagtcc accggcttct tcaccgacgc caccaaggcg
      721 cacgcccaca tcgacggtgg cgcgaagaag gtcatcatct cggcgccggc ctccaacgag
      781 gacgccacgt tcgtcatggg tgtcaaccac caggactacg acccggccaa ccaccacgtc
      841 atctccaacg cgtcgtgcac gacgaactgc ctggccccca tggccaagcc gctgtccgac
      901 gagctcggca tcgtcaaggg cctgatgacc acggtgcacg cctacacctc cgaccagaac
      961 ctgctcgacg gcccgcaccg cgacatgcgc cgcgcccgcg ccgccgcgct gtcgatcatc
     1021 ccgacgagca ccggtgccgc caaggccatc ggcctggtca tgcccgagct gaagggcaag
     1081 ctcgacggct acgcgctgcg cgtgcccacc ccgaccggct cggccaccga cctcaccttc
     1141 gaggccggcc gcgagaccac cgtcgaagag gtcaacgcga tcgtcaaggc tgctgccgag
     1201 ggtccgctca agggcttcct gaagtacacc gaggacccga tcgtctcggc cgacatcgtc
     1261 accgacccga gctcgtgcat cttcgactcc ggtctgacca aggtcatcgg aacccaggtc
     1321 aaggtcgtcg gctggtacga caacgagtgg ggctactcca accgcctcat cgacctgacg
     1381 ctcctcgtcg gcaagagcct ctgagagcgc ggggccgagt cgatcatggg tgacctctcg
     1441 tcgttgggcg acgtgcgcgg caagcgcgtc ctcgtccgct ccgacctcaa cgtgccgctc
     1501 gacggcacga cgatcacgga cgacggccgc atccgggcct cggtcccgac catctccgcc
     1561 ctggccgagg cgggcgcacg cgtcatcgtg tgcgcccacc tcggtcggcc caagggtgcc
     1621 ccggaggaca agtactccct ggcgcccgtc gtcgggcggc tctccgagct gctcggacgc
     1681 ccggtggcct tcgccaccga caccgtcggc gagagcgcgc gcgcgaccgt cgacgcgctg
     1741 ggggacggcg acgtcgccct gctcgagaac ctgcggttca acgcggggga gaccgccaag
     1801 gtcgacgagg agcgtgccga gttcgccgac gccctcgccg ccctggccga cgcctacgtc
     1861 tccgatggtt tcggggtcgt gcaccgcaag caggcatcgg tctacgacat cgccacgcga
     1921 ctgccgtccg ccatgggtgg cctcgtcgcc accgaggtcg acgtcctcaa gcgcctgacc
     1981 gtcgaccccg agcgcccgta tgccgtcgtg ctcggtgggg ccaaggtcgc cgacaagctc
     2041 gccgtcatcg acaacctcat gaacaccgcg gatcgcctcc tcatcggggg cggcatgctc
     2101 ttcaccttcc tcgcggccca gggccacgag atcggtaaga gcctgttcga cgccgacagc
     2161 gtcgacgcgt gtcgtgacta cctgcgtcgc gcggaggaga ccggcgtcga gatcgtgctg
     2221 cccgtcgacg tcatctgcgg tcgcgagttc tccgccgaca ccgagaccgt caccgtggcc
     2281 gccgacgcca tccccgccga catgatgggc ctggacatcg gcccggcctc ggcgcagctg
     2341 tatgccgaga agctcgccga cacgcgcacg gtcttctgga acgggcccat gggcgccttc
     2401 gagatggccc cgttcgccga aggcacgcgc cgtctcgccc aggcgctggt cgacgtcact
     2461 gcccagggtg ccctcaccgt ggtcggcggt ggcgactccg cagccgcggt gcgccagctc
     2521 ggcttcgagg acgcccagtt cggacacatc tccaccggtg gtggtgcctc cctcgagtac
     2581 ctcgagggca aggaactgcc cggtatcgcc atcctcacga aggagcactg acgcatgacc
     2641 tccggccgca cccccctgct cgcgggcaac tggaagatga acctggacca cctccaggcc
     2701 acgcacctcg tgcagaagct cgactggacg ctcaaggacg gcaagcacga cttcggcgcg
     2761 gtcgaggtcg cggtgctgcc gccgttcacc gacctgcgca gcgtgcagac cctcatcgac
     2821 ggtgaccggc tcgagctgaa gttcggcgcg caggacctct cggtgcacga cgccggggcc
     2881 tacaccggtg agatcagcgg cgcgttcctc gccaagctgg gctgctccta cgtcgccgtc
     2941 gggcacagtg agcgtcgcga gtaccaccac gaggacgact ccgtggtcaa cgcgaaggtc
     3001 aaggccgcct atcggcacgg catcacgccg atcttctgct gcggcgaggg cctggagatc
     3061 cgtcaggccg gcacccacgt cgagcacgtc ctcgcgcagg tcgaggccgg gctggacggc
     3121 ctgtccgtcg aggacgcccg cagcatcgtc gtcgcctacg agcccatctg ggcgatcggc
     3181 accggcgagg tggcgacccc cgaggacgcg caggaggtct gcgcggcgat ccgtaccaag
     3241 ctcgccgagc tctacagcgg cgacgtggcg gacggcatcc gggttctcta cggcggttcg
     3301 gtgaagtcga gcaacgtggc ggccatcatg gccaaggagg acgtcgacgg cgccctcgtc
     3361 ggcggggcct cgatcgaccc ggccgagttc gccgcgatct gccgcttccg ggaccacctc
     3421 gccacggcct gacgtccccc tgaccggggt cgccgcctcc agcggttatc cttgagcggt
     3481 tgtccccccg atctcgcacg aaggactctc acgtggatgc ggttcgcatc ggcctccagg
     3541 tactcctggt cctcggtggc ctgttcttga ccctgctcat cctgatgcac aagggcaagg
     3601 gcggtggcat gtccgacatg ttcggtggtg gcatgtccag ctcgctcggt gggtcgtcgg
     3661 ttgccgagcg caacctcgac cggatcacca tcggggtggc cctcgtgtgg ttcacctgtg
     3721 tcgtgggtct gggcgtcctc gaacgcttcg ccgtctaggt cagggagcag tcatggcagg
     3781 tggaaacgcg attcgtggtt cgcgcgtcgg tgcgggcccc atgggtgagg ccgagcgcgg
     3841 ggaggttgcc cctcgggtgc acatctcgtt ctggtgctcc aacgggcacg agacccggcc
     3901 gagcttctcg caggagcccg gtctggtcat ccccgaacag tgggactgcc cgcgctgcgg
     3961 gttccccgcc gggcgcgacc aggacaaccc gccggcaccg atgaaggtcg agccgtacaa
     4021 gacgcacctg gcctacgtga aggaacggcg ctccgacgtc gacggcgagg cgatcctcga
     4081 cgaggccctg ggctcgctgc gtgaccgcgg cctgatccag taacagcggc cgctccggca
     4141 ggagcggtca gccccggctg gcgcgggcca ggtgtccgac gactccgggg atccagcccc
     4201 gttcccgccc catccagcgc accgcgacca tcgtgcgggt cacgacgggg atgccgtggc
     4261 gcggcggttc gacggccagg tcggttccga aggggtcggt gctgaccacc cagcggtgac
     4321 ccgcagcagc cacgaggtgg tcaccgatgg ccgtgctgat gagggtgacg acgtccgcgg
     4381 acgcgatggc gccgtcgtcg gcctcgaccg cccggtcgta ggccgctgcg atgctctgga
     4441 ggtcgctcgg ctcgatgccg tgggcagcgt agccggctcg ggcgcggtcc agcgctgcct
     4501 cgtcctgcgc agtcagccgt cggatgccga tctcctcgag ggcctccacg ccggagtcgt
     4561 cagccgtgac gtcgtcagcg ctcgcggcgg cgtggtcggg ggtggagtcg gcgtcgcgtc
     4621 cgcgcagtcg gtcgaacagt cccatcgcct caaccttcca ggtcgccggc agcggcgtcg
     4681 tcgacgagcc acagggtgcg ctcgatgccg tggacgtggg ctgccggggt ggtctcgaac
     4741 ggggctcccg tggtgcctcg gagcaccgcg tcggccttgt ccgccccgct gacgaggaac
     4801 cagacctcgc gggagcgctc gaggcactcc cgagtcagcg atacccgggt gggaggcggc
     4861 ttgggggagt cgtgcaccgc gatggcgatg ccggcggtgg aggcggccgc ggggtggtgg
     4921 gggaacagtg aggccacgtg accgtcgggg cccaccccca gcaccatgac gtcgaacatc
     4981 ccaccgccgt gctcgcgcag cgcctgctcg tacagggccg cactggcctc ggcgctctca
     5041 ctggtgtccg ggccggcaac tcggtgcacg tgctcgggtg tcaggcccag cgtggccagc
     5101 ccggcctggt cgttctgggt gtcgttgcgg tccgggtcac cggcaggcag ccaccgttcg
     5161 tcgccccacc agacgtgaac ctgagaccag tcgacggcat cgcgcgcggg caccgcgcac
     5221 aacgacgaga tgatctgcga ccccatcgac ccgccggtca tcgagatctg ggcgacgccg
     5281 cgggcacact gggcgtcgac gatcgcgacg acgaggcgcg ccgcggcggc atccgccagg
     5341 gcctgtcgac cggggtggac gacgacggtg gtgggaccgg tcatgatcga cgctccttga
     5401 cctcggcgag cttgtccttg gcggcctgct tgacgacctc cgggtcatcg accttcggcg
     5461 gcatcggggc ggcctcgacc atggcactgc gggtgctggc ccgcgctgcc cttcgtagcc
     5521 gcttcgcggt gcgctcggcc tccgcgaccg agggagcccg ccccgcccgc acggcctcgc
     5581 tggcggtctg gcgcgacacg gtgcaccggc gaagtcccac gacgagggcg tcgtggtaga
     5641 cctcgtcggg gtccaggcgc cgcagctcgt cggcgaggca ctccgcgtcg ccgcggtgcg
     5701 caagcgccag agttcgcacc ggctggcccg gctgggccag ggtcgcggtg ttgccgtcga
     5761 ccggcctcac gaggtcgatc gacccgctgt cgcgctccag tcggaccgag acgatgccgc
     5821 tcccggaacg cgcccgggcg atggacacgg ggcacttgag gcgagcggcc agccacccgg
     5881 cgagcaggtc acccgagggg gagtccgccg cagcgacgac gaccgcgctc gtgacgggct
     5941 cgtacggtgg ctggtccagg gctgcggtga gcaggccgcg ccacaaggtg atcctcgacc
     6001 aggcgaggtc gctgtcaccg gccgcgtacc cgtcggacag gcggcgcagg accgttcggg
     6061 acgtggtgct gaaccgggcg gcgtcggtga cgcggcgctg ggccatcatg ccgatcgggt
     6121 ccttggacgg ttcgtgggga ccgttggccg gccaccacgc gacgatgggg gagtcggcga
     6181 ggagcagtgg ggtcacgact gcccggccgt gggaggccag tcgaccgaag aggcgaagga
     6241 ccacgacctc ggaggccccg gcatccccac ccaccctgat ctgcccgtcg agccgtgcgg
     6301 caccacgttt gctgcccgcg atgacgacga tgatccgaca ggggtgttgg cgactcgcgt
     6361 cgttcgcggt ctcgatcgct gcgtcggcgt gggccgcgtc gacgacgacg agcagggtca
     6421 gcacccgcga gagcgccatc gacccggtgt cggcgcgcag ccgaaccagt cgcgccgaga
     6481 tggcggcggt ggtcgtgtcg ggcaggtcga cgatcatggc agcctccact ctcgtccgtc
     6541 gcgctgcatc atggcatcgg cgctggcggg tccccacgtg ccggcggggt actgctcggg
     6601 cgcgcgcccc ttcgtggccc agtacttctc gatggggtcg aggatctgcc aggagagttc
     6661 gacctcctcg tggcggggga agagcggtgg ctcgccgagg aggacgtcga ggatgaggcg
     6721 ctcgtaggcc tctggcgagg actcggtgaa ggcccggccg taaccgaagt ccatcgtcac
     6781 gtcgcgcacc tccatctggg cgccgggcac cttggcgccg aagcgcaggg tcacgccctc
     6841 gtcgggttgg acccggatga cgatggcgtt ctgcccgagc tcctcggtgg ccgtgtcggt
     6901 gaacggcagg tggggtgcct tcttgaagac caccgcgatc tcggtgaccc gcttgcccag
     6961 ccgcttgccc gtgcgcaggt agaacgggac accggcccag cggcgggtgg cgacgtcgag
     7021 tcggatggcg gcgtaggtct cggtgcccga gcccttggcg accccggact cctcgaggta
     7081 gccgaccacc tcctcgccac cctgccaccc ggcggagtac tgcccccggg cggtgcccgt
     7141 ggacaggtcg gcggggatcc tcacggcgga gaggaccttc tccttctcga gccgcagacc
     7201 gtgggcgtcg aaggatgccg gctcctccat cgcggtcagc gcgagcagct ggagcaggtg
     7261 gttctggatg acgtcacgag cggcgccgat gccgtcgtag tacccggcgc gcccgccgat
     7321 gccgatgtcc tcggccatgg tgatctgcac gtggtcgacg tagttggcgt tccagacggg
     7381 ttcgaacatc tggttggcga aac
//
LOCUS       DAOSOC010000127         7366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_10112_length_7366_cov_9.629326, whole genome shotgun sequence.
ACCESSION   DAOSOC010000127 DAOSOC010000000
VERSION     DAOSOC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10112_length_7366_cov_9.629326"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PL014_09680"
     CDS             <1..1335
                     /locus_tag="PL014_09680"
                     /inference="COORDINATES: protein motif:HMM:NF022113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HOT57403.1"
                     /translation="LETVGGQWRIAALPEGFGRWIASTEVNRLVRPYAVHYVSTSQRA
                     LIPDVRWFPVDRLATRLARAQILPLPEHLDGAAVTAVPSGARLLGDAVSINSGVATVN
                     LISSKLAPGQTTRQNLWAQFVATLTQDPAVTRVSLSVDDVPVNLLTLDGSAGTLAEIG
                     FIPPPPVTLAAPVVRRGEEVAVFDPSSLGDQEPREPADQRAYPAVPAGFSRLALSADG
                     SEVAAINPGGDGISRWRDANRYVVPLPGGAVGSPSYDRRGWLWVGAVGAGGAGAPRLF
                     VVNTASDPAGGDAAAVAIEAAWLAGRRVLEARVSPDGDRIAVLSTAADGRDARIDLTG
                     IVRSRAGQPQRLASPLRLGASLSRATSLAWLDERTLTALGVLEGSMLQPVIVTVGGGV
                     RALTAAPDAVAITSTGGERALWVTTSTGRLLGRAGSQWVDSGPATDLAVASG"
     gene            1412..2179
                     /locus_tag="PL014_09685"
     CDS             1412..2179
                     /locus_tag="PL014_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT57404.1"
                     /translation="MGDRLWERVVGAAAAAGELVLPSRCAACHRPGPPLCRACTQTLR
                     TASEGWPALVTLDPAPAVMPACWAAAPFDGPLRMAVTAYKDEERRDLRPALAQLLASA
                     LTTAFAADPTLRHHVSRGEPVLLVPLPTARSSRRRRGDDPVGDLARAGARGCPPGIHL
                     APALRHTRRVADQARLDRGARAANLAGAIEVSRRWRQVVRGSTCVLVDDVVTTGATMA
                     EATRALRAVGAGHVVAAAVAATPRRVTPALVAHRVAD"
     gene            2271..2924
                     /gene="raiA"
                     /locus_tag="PL014_09690"
     CDS             2271..2924
                     /gene="raiA"
                     /locus_tag="PL014_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT57405.1"
                     /translation="MDISVTGRHLNVSDRFRAHIEEKLAKITQLEPRTRRVEVLISHE
                     PNRRQAKACDKVEITCYAKGPVIRAEACEEDKHAALDVAIDKLMERLRRQHDRRQVHR
                     GRRTPESVAAATARIVPPAEQSVADAAPEVDEPDGPFGDSPVEVREKIHRSSPMTLAD
                     ALREMELVGHDFYLFHDTDTDRASVVYRRRGWSYGVLHLDLDDSPQDGLADDEAAAS"
     gene            3016..3696
                     /locus_tag="PL014_09695"
     CDS             3016..3696
                     /locus_tag="PL014_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT57406.1"
                     /translation="MQSVSPGAVTRVVIADDHAQYRRGMQIVVELEGTARVVGEASNG
                     DEALAVCTRLRPDIVLMDVRMPGVGGIEACRRIRWAVPETRIIMLTMSDEEDDLFEAI
                     KAGASGYLLKDVPGEEVLAAIGRVGEGQAIIPASMAATLLTEFARLSHDPEPLQRSLP
                     PLTDREVEVLRLVARGQANREIAEGLVISENTVKNHVRNILEKLHLHSRVEAAVYAHQ
                     KHLIPTDE"
     gene            3689..4894
                     /locus_tag="PL014_09700"
     CDS             3689..4894
                     /locus_tag="PL014_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOT57407.1"
                     /translation="MSDAPASVRLSQRAARRIALAAQGFGRPRPESPGIRDVQRVIDT
                     VTVVQIDSVNVVSRSHYLPFFSRLGVYDPALVDAARDRAPRRLVEYWAHEASLVAPST
                     WPLLDFRMRRAREDAWGNMRHVARENPDLVEAVLDEVARRGPLTSREVEAALAHDLPR
                     QRDDWGWNWSVVKSALEHLFWAGEVSSAGRTAQFERRYAVPARVLPPALRALALDPDA
                     RPSEDESFRRLIEMSARAHGVGTEQCLRDYFRLRPEQARPAIAALVAEGTLVPAEIEG
                     WRRPAYLHRDARRPRGIRARALLSPFDSLIWQRDRTSALFGFDYRLEIYVPEHLRVHG
                     YYVLPFLLGEDLVARLDLKADRAAGVLRARRCTWEPGRGGAEDQRELDEELALLATFL
                     GLTRTQIDQ"
     gene            complement(4913..6052)
                     /locus_tag="PL014_09705"
     CDS             complement(4913..6052)
                     /locus_tag="PL014_09705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT57408.1"
                     /translation="MTRMPFPGCVPELTDGVVRLRAHRPDDAEAIVEQSIDPESVRWT
                     TVPRPYALEQAREFLAIIESGWMAEDGNRLWAISDAADPQARFLGTIDVRPIAGGAAS
                     IGYGLHPDGRGRGLMAGALRLVCRWWFEQGGVRIHWEAERGNFASWRVAWACGFTHHG
                     TTPQASVDPAGGTAIDMWRGSLGADDVMDPRTPWADPPLLTADGGNGILLRPWSDDDV
                     THLEGRDQPAHYMPARGVLDADTFADWLLARRERMSLGVAQSWCIADAESDAALGEVL
                     VFVTEGTLEDDTAELGYQVLPSARGRGVATAAAQAVVEHAFTPRSDGGLGMRRLVAQT
                     AEDNAASNAVLDRLGFTIWGRETAADVLPGGKAIDALHWELLRSP"
     gene            6228..>7366
                     /locus_tag="PL014_09710"
     CDS             6228..>7366
                     /locus_tag="PL014_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT57409.1"
                     /translation="MKIVEKLLRAGEGRILKKLDNLAAQVNAIEEDFEKLTDAELRAE
                     TDVFKKRLADGETLDDILPEAFAAVREASKRTLGKRHFDVQIMGGAALHMGNVAEMRT
                     GEGKTLVATLPSYLNALTGKGVHVITVNDYLAEYQSELMGRVHRFLGLETGCILASMT
                     PEQRRAEYAKDITYGTNNEFGFDYLRDNMAWSVDELVQREHNFAIVDEVDSILIDEAR
                     TPLIISGPADAPAKWYVEFAKIAARLERGELESGRGDYEVDEKKRTVGVLESGIEKVE
                     DHLGIENLYESANTPLIGYLNNAIKAKELFQRDKDYVVLNGEVLIVDEHTGRMLAGRR
                     YNEGMHQAIEAKEGVEIKNENQTLATITLQNYFRMYDKLSGMTGTA"
BASE COUNT         1084 a         2702 c         2574 g         1006 t
ORIGIN      
        1 ctcgagaccg tgggcgggca gtggagaatc gccgcccttc ccgagggttt cggccgctgg
       61 atcgccagca ccgaggtcaa ccggctcgtg cgcccctacg ccgtgcacta cgtgtcgacg
      121 agccagcgag cgctcatccc ggacgtgcga tggttccccg tcgatcgcct ggccacccgg
      181 ctggcgcgcg cccagatcct gcccctgccc gagcacctcg acggtgctgc cgtcacggcg
      241 gtcccgtctg gtgcgcggct gctcggcgac gccgtctcga tcaactcagg ggtcgccacg
      301 gtcaacctca tctcgagcaa gctggcgccc ggccagacga cgaggcagaa cctgtgggcg
      361 cagttcgtcg ccacgctcac ccaggacccg gcggtcacga gggtctccct ctccgtcgac
      421 gacgttcccg tcaacctgct cacgctcgac ggttcagccg gcacgttggc cgagatcggg
      481 ttcatcccgc cgccgcccgt caccctcgct gccccggtgg ttcggcgcgg cgaggaggtc
      541 gcggtcttcg atcccagcag cctcggggac caggaaccgc gcgagcccgc cgaccagcgc
      601 gcctaccccg ccgtgcccgc agggttctcc cgactggccc tgtccgcgga cggcagcgag
      661 gtggcggcga tcaacccggg cggtgacggc atctcccgct ggcgcgatgc caatcgctac
      721 gtcgtgccgc tgcccggcgg tgccgtcggg agcccgtcct acgaccgccg ggggtggctg
      781 tgggtgggtg ccgtcggggc cggaggtgcc ggcgcgccgc gactgttcgt cgtcaacacg
      841 gcatccgacc cggccggggg cgacgccgct gccgtcgcca tcgaggcggc ctggctcgcc
      901 gggcgcaggg tgctcgaggc tcgagtgtcc cccgacggcg accgcatcgc cgtgctgtcc
      961 acggcggccg acggccgcga cgcgcgcatc gacctcactg gaatagttcg ctcgcgcgcc
     1021 ggtcagccgc agcgcctcgc gtcgccgctg cgcctcggcg cctcgttgtc gagggcgacg
     1081 agcctggcct ggctcgacga gcgcaccctg accgctctcg gggtcctcga gggcagcatg
     1141 ctccagccgg tcatcgtcac cgtcggcgga ggcgtgcggg ccctgacggc ggccccggat
     1201 gccgtggcca tcacctccac gggcggcgaa cgcgccctgt gggtcaccac gtccaccggc
     1261 cgcctgctcg gccgggcagg gtcccagtgg gtcgacagcg gcccggcgac cgacctcgcc
     1321 gtcgcgtccg gctgacccga ccgtccacag ggggcgatct cgaccggcgc cagcacgtgg
     1381 tgtggggtca tgctggggcg cagggggtgc catgggggac aggctctggg agcgggtggt
     1441 gggcgcagcg gccgcagccg gtgagctcgt gctgccgtcc cggtgcgcag cctgtcaccg
     1501 acccggccca ccgctctgcc gagcctgcac gcaaaccctg cgcacggcat ccgaggggtg
     1561 gcccgcgctc gtcaccctcg atccggcacc ggccgtgatg ccggcctgct gggcggcggc
     1621 gccgttcgac gggccgctgc ggatggccgt gaccgcctac aaggacgagg agagacgtga
     1681 cctgcgcccg gccctcgccc agctgctcgc gtccgccctg acgacggcct tcgcagcaga
     1741 ccccacgctg cgtcaccacg tctcgcgggg tgagcccgtg ctgctcgtgc cccttccgac
     1801 cgcgcgttcc tcgcggcggc ggcggggcga cgacccggtc ggggacctcg cccgggccgg
     1861 tgcgcgtggt tgcccacccg gcatccacct cgcgccagcc ctgcggcaca ccaggcgggt
     1921 ggccgaccag gcccgcctcg accggggtgc gcgggcggcg aacctcgccg gcgcgatcga
     1981 ggtctcgcgg cggtggcgcc aggtcgtcag gggatcgacg tgtgtgctcg tcgacgacgt
     2041 cgtgaccacc ggagcgacga tggcggaggc cacacgggcg cttcgcgccg tgggcgcggg
     2101 ccacgtcgtc gccgccgcgg tggccgccac cccgcgccgt gtgacccccg cccttgtggc
     2161 ccatcgggtg gccgactagc gtcagtgcat gagccccgat tgtgcggagg tggtgccgag
     2221 attcgccgta gagcactgag caacaacccc cccagcacaa ggaggaccac gtggacattt
     2281 ccgtcaccgg acggcacctg aacgtctctg atcgattccg tgcgcacatc gaggagaagc
     2341 tggcgaagat cacccagctc gagccccgca cgcgtcgagt ggaggttctg atcagccacg
     2401 agcccaaccg ccggcaggcc aaggcctgcg acaaggtgga gatcacctgc tacgccaagg
     2461 ggcccgtcat tcgggccgag gcgtgcgaag aggacaagca cgcagccctc gacgtggcga
     2521 tcgacaagct gatggaacgg ctcaggaggc agcacgaccg gcgacaggtc caccgagggc
     2581 gtcgaacccc cgagtccgtg gcggccgcga ccgcccgcat cgtgccgccg gcggagcagt
     2641 ccgtggccga cgccgccccc gaggtcgacg agccggacgg cccgttcggc gactcgccgg
     2701 tcgaggttcg cgagaagatc caccgctcct cgccgatgac gctggctgac gccctgcgcg
     2761 agatggaact cgtcgggcac gacttctacc tcttccacga caccgacacc gaccgcgcga
     2821 gcgtggtcta ccgccgccgt gggtggagct acggcgtgct gcacctcgac ctcgacgaca
     2881 gcccgcagga cgggctcgcc gacgacgagg cagccgcctc ctgagggcgc gcaggtccgg
     2941 gtggtgggca acaccgcccg gacctgccac catgttctcg tgcagggtga cgtagggacc
     3001 gtgaacggag accgcgtgca gtcagtttcc ccgggggcgg tgacccgtgt ggtcatcgcg
     3061 gacgaccacg cccagtaccg gcgaggcatg cagatcgtcg tcgagctcga gggcaccgcg
     3121 cgcgtcgtcg gcgaggcatc caacggtgac gaggccctgg cggtctgcac ccggctgcgg
     3181 cccgacatcg tgctcatgga cgttcggatg ccgggcgtcg gcggcatcga ggcctgccgc
     3241 cggatccgct gggcggtgcc cgagacccgc atcatcatgc tcaccatgtc cgacgaggag
     3301 gacgacctct tcgaggcgat caaggccggc gccagcggct acctgctcaa ggacgtgccg
     3361 ggtgaagagg tcctggcggc gatcggtcgg gtcggcgagg ggcaggcgat catcccggcc
     3421 agcatggctg ccacgctgct gaccgagttc gcccggctca gccacgaccc cgaaccgctc
     3481 cagcgctcgc tgcctccgct caccgatcgc gaggtcgagg tgctgcgcct cgtcgctcgg
     3541 ggccaggcca accgcgagat cgccgagggg ctggtcatct cggagaacac cgtgaagaac
     3601 cacgtgcgca acatcctcga gaagctgcac ctgcactcac gggtcgaggc cgccgtctac
     3661 gcgcaccaga agcacctgat ccccacggat gagtgacgcg cccgcgagcg tgcgtctgtc
     3721 gcaacgggcc gctcgccgca tcgcgctcgc tgcccagggg ttcgggcggc cgcgccccga
     3781 gagccccggc atccgtgacg tccagcgggt catcgacacc gtgaccgtcg tgcagatcga
     3841 cagcgtcaac gtcgtctcgc gcagccacta cctcccgttc ttctcgcggc tgggggtgta
     3901 cgaccccgcg ctcgtcgacg ccgcccgtga ccgggcgccg agacgcctcg tcgagtactg
     3961 ggcgcacgag gcctcgctcg tcgcaccctc gacgtggccc ctgctcgact tccggatgcg
     4021 gcgggcccgc gaggacgcgt ggggcaacat gcggcacgtc gcgcgcgaga accccgacct
     4081 cgtcgaggcc gtgctcgacg aggtcgcccg gcgcgggccg ctgaccagcc gcgaggtgga
     4141 ggccgccctg gcccacgacc tgcctcgcca gcgcgatgac tggggctgga actggtccgt
     4201 ggtcaagagc gccctcgagc acctgttctg ggcgggggag gtgtccagcg ccgggaggac
     4261 ggcgcagttc gaacggcgct acgcggtgcc ggcgcgggtg ctgccaccgg cgctgcgtgc
     4321 cctcgcgctc gacccggacg cccggccctc ggaggacgag agctttcgcc ggctcatcga
     4381 gatgtccgct cgtgctcacg gtgtcggcac cgagcagtgc ctgcgtgact acttccggct
     4441 gcgccccgag caggccaggc cggccatcgc ggcgctcgtg gccgagggaa ccctggtgcc
     4501 ggccgagatc gagggctggc gacgacccgc ctacctgcac cgagacgccc ggcgaccacg
     4561 cggcatccgg gcgcgggccc tgctctcacc gttcgactcc ctgatctggc agcgcgaccg
     4621 cacgagtgcc ctcttcgggt tcgactaccg tctcgagatc tacgtgcccg agcacctgcg
     4681 cgtgcacggg tactacgtgc tgccgttcct gttgggggag gacctcgtcg cgcgcctgga
     4741 cctcaaggcc gaccgggcag cgggggtgct gcgggctcgg cggtgcacgt gggagcccgg
     4801 ccgcggcggt gcggaggacc agcgagagct cgacgaggaa ctcgccctgc tggcgacctt
     4861 cctcggcctc acccgtaccc agatcgacca ataggtcact gggggtcgcg ggtcaggggg
     4921 agcggagcag ttcccagtgc agggcgtcga tggcctttcc gccgggcagc acgtcggcag
     4981 cggtctcgcg cccccagatg gtgaacccca accggtcgag cacggcgttg ctggccgcgt
     5041 tgtcctcggc ggtctgggcc acgagccggc gcatgccgag cccgccatcg ctccggggcg
     5101 tgaacgcgtg ctcgaccacc gcctgcgcgg cagcggtcgc gaccccgcga ccgcgtgcac
     5161 tgggcagcac ctggtagccc agctcggcgg tgtcgtcctc gagggtgccc tcggtgacga
     5221 acacgagcac ctcacccagt gcggcatccg actcggcgtc ggcgatgcac cagctctggg
     5281 ccacgccgag cgacatccgc tcgcgtcgtg cgagcagcca gtcggcaaag gtgtcggcgt
     5341 cgagcacgcc ccgcgcaggc atgtagtgcg ccggctggtc gcggccctcg aggtgggtga
     5401 cgtcgtcatc gctccacggg cgcagcagga tgccgttgcc cccgtcggcg gtgagcagcg
     5461 gtgggtcggc ccacggggtc cgggggtcca tgacgtcgtc ggcgcccagg ctgccccgcc
     5521 acatgtcgat cgcggtgccg cccgcagggt cgaccgaggc ctggggggtg gtgccgtggt
     5581 gggtgaaacc gcacgcccac gccacccgcc acgacgcgaa gttgccgcgc tcggcctccc
     5641 agtggatccg gaccccgccc tgctcgaacc accaccggca gaccaggcgc agggcaccgg
     5701 ccatcaggcc ccggccccgc ccgtcggggt gcagcccgta gccgatgctg gccgcgcccc
     5761 ccgcgatggg gcgcacgtcg atggtgccga gaaagcgcgc ctgcgggtcc gcggcatccg
     5821 agatcgccca gagccggttg ccgtcctcgg ccatccagcc ggactcgatg atcgcgagga
     5881 actcgcgggc ctgctccaac gcataggggc gcggaacggt ggtccaccgg acgctctcgg
     5941 ggtcgatgga ctgctcgacg atggcctcgg catcgtcggg gcggtgggcg cgaaggcgca
     6001 ccacaccatc ggtgagctcg ggcacgcacc cggggaacgg catcctcgtc atgcccccca
     6061 cccaaccatc cgactcctcc ccggggaacc gcattttgtg ctgcgtcgtt ggcctgaccg
     6121 gtgagtcgga cacgggtccg gcggccgtcc gcctgctcga ctaccctagg caggtccacc
     6181 ccgtggtggt cactgccgca cgtccgctgc ctgaggagtc ccctgccgtg aagatcgtcg
     6241 agaagctgtt gcgcgccggt gaaggccgca tcctcaagaa gctcgacaac ctcgcggccc
     6301 aggtcaacgc gatcgaggag gacttcgaga agctgaccga cgccgagctg cgggccgaga
     6361 cggacgtctt caagaagcgc ctcgctgacg gtgagaccct cgacgacatc ctgcccgagg
     6421 cgttcgccgc ggtgcgcgag gccagcaagc gcacgctcgg caagcggcac ttcgacgtgc
     6481 agatcatggg cggcgcggcg ctgcacatgg gcaacgtcgc cgagatgcgc accggtgagg
     6541 gcaagaccct cgtcgcgacc ctgccgtcct acctcaacgc cctgaccggc aagggcgtgc
     6601 acgtcatcac cgtcaacgac tacctcgcgg agtaccagtc cgagctcatg gggcgcgtgc
     6661 accgcttcct cggcctcgag accggctgca tcctcgcgtc gatgacgccc gagcagcggc
     6721 gcgccgagta cgccaaggac atcacctacg gcacgaacaa cgagttcggg ttcgactacc
     6781 tgcgcgacaa catggcctgg tcggtcgacg agctcgtgca gcgcgagcac aacttcgcca
     6841 tcgtcgacga ggtcgactcg atcctcatcg acgaggcgcg cacgcccctc atcatctccg
     6901 gcccggccga cgccccggcc aagtggtacg tcgagttcgc gaagatcgcc gcccgcctgg
     6961 agcgcggcga gctcgagtcc ggccgcggcg actacgaggt cgacgagaag aagcgcaccg
     7021 tcggcgtgct cgagagcggc atcgagaagg tcgaggacca cctcggcatc gagaacctct
     7081 acgagtcggc caacaccccg ctcatcggct acctcaacaa cgccatcaag gccaaggagc
     7141 tgttccagcg cgacaaggac tacgtcgtgc tcaacggtga ggtgctcatc gtcgacgagc
     7201 acaccgggcg catgctcgcc ggccgccgct acaacgaggg catgcaccag gccatcgagg
     7261 ccaaggaggg ggtggagatc aagaacgaga accagaccct cgcgacgatc accctccaga
     7321 actacttccg catgtacgac aagctctcgg gcatgaccgg cacggc
//
LOCUS       DAOSOC010000128         7211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_10382_length_7211_cov_9.924259, whole genome shotgun sequence.
ACCESSION   DAOSOC010000128 DAOSOC010000000
VERSION     DAOSOC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10382_length_7211_cov_9.924259"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PL014_09715"
     CDS             <1..261
                     /locus_tag="PL014_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57410.1"
                     /translation="HSPRNGGCSPSSCCSSSSGTCVFTQITLLQDGGGVANETNLLGT
                     YVDHLGTGKGDFGTASAVSILILALTIALCWYYVRSLTKEGS"
     gene            258..581
                     /locus_tag="PL014_09720"
     CDS             258..581
                     /locus_tag="PL014_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57411.1"
                     /translation="MNPRSPLARTGLSVLALALAAIWGFPVYWMLNSAFLPNVVLQST
                     DPTLLPFGGSFDNLRVVVTDPAFLSALGTSLVVTLTTVVARSCSRCCSSRCCPPRGCS
                     SPSTR"
     gene            complement(628..756)
                     /locus_tag="PL014_09725"
     CDS             complement(628..756)
                     /locus_tag="PL014_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57412.1"
                     /translation="MQDQFSAVEPDAEVGELGSDAGSDPATKPRSIQIVKGTTAAV"
     gene            complement(776..1666)
                     /gene="htpX"
                     /locus_tag="PL014_09730"
     CDS             complement(776..1666)
                     /gene="htpX"
                     /locus_tag="PL014_09730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOT57413.1"
                     /translation="MHRHHNGLKTAALFGGMWAILLGIGWVVAQMTGSSVFLLVFAFI
                     GVATTFYGYWNSDKLAIKAMHAQPVSEAEAPQMYRIVRELSTKAGKPMPRLYISPTQA
                     PNAFATGRNPENAAVCCTQGILGLLDERELRGVLGHELMHVYNRDILTSSVAAAIAGV
                     ISTVAQFLSWGMIFGGGGSDNENRNPIAGLALAILAPFAAMVVQLAISRTREYDADQD
                     GAELTGDPLALASALRKLELGVARAPLAPTQDLVNASHMMIANPFRAQDISRLTATHP
                     PMAERIARLEQMAYGHRINP"
     gene            complement(1704..3212)
                     /locus_tag="PL014_09735"
     CDS             complement(1704..3212)
                     /locus_tag="PL014_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT57414.1"
                     /translation="MTAAISTASLVSIDAVALAPALLPAIGVVLVLLGDALLPGRRDW
                     QVGLGLAVLVVGAVGALISAVQAADGAVRTLCLPAPEGACLWTASPMTGMLQAGILAA
                     TAAALALLHDRDGGPRDTTVTTALLLAAATGGAGVAASRDLGTWLVTLELATIPLVAL
                     VALRGTRSAAHGALTLLVTSVTSFALLVLGVALWMTATGDAAFGTDTVALAWADPHRR
                     AVLVLAAVVLLAGLGFKLSLVPFHAWTPQAYSTADLGTATALAAASKVSAAAALLVVV
                     QAFVGVPGDLRQVSIVIGALAAASMLLGNVMALRQDDVTRLLAWSTVAQAGWVVLPVA
                     ALTDAGLHAAAVYVLTYAVATVVAFAAVAAVGAGPLSRMRGLLRRDRLTGGALAFALL
                     VLAGLPPGVIGLVAKVVALRPAVEQGLWPLALVAVVAVVLGIAVYLRWFAVLIGDAGA
                     RGDAGEMSEPGAATHEVSTPASGGGARAVLVAGTVLLVLLSALPGLLLGLVS"
     gene            complement(3209..>3985)
                     /locus_tag="PL014_09740"
     CDS             complement(3209..>3985)
                     /locus_tag="PL014_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT57415.1"
                     /translation="PTAGSVLLAAVLLKMGTYGLVRLPVATVPDGFARLAPVLAVLGV
                     VGIIWGGLICLVERDLKRLIAYSSVAHMGFVVLALATGSETGLQAALFANIAHGVISA
                     LLFFLVGGLKARWGSVDLTVPRPALRESSPRLGFLLVLGLAASLGLPGLAGFWGEFFA
                     VYAAWSPADGRSRALFIACAVVAAVGAALAAAYALRVARTVWVGDALQEGERVTPDAD
                     GTELGILGVLAASVILLGVFPGIVLDRTADAVSQLIGTWP"
     assembly_gap    3986..4085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4086..4539)
                     /locus_tag="PL014_09745"
     CDS             complement(<4086..4539)
                     /locus_tag="PL014_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOT57416.1"
                     /translation="MISLLLALPAAVGLWLVTAGRDVSVAVARLVTAVAAVLTLGLAV
                     VVSAAGSSVDRAWLPDLGVRWTFGVDGIAVPLVLLTAAIGVLVAAHAWVDLPAGGSPA
                     AFLGCLLLVEAGALATFTARDAVLFFVAFELVLVPMWVLISRYGDPHDA"
     gene            complement(4536..6377)
                     /locus_tag="PL014_09750"
     CDS             complement(4536..6377)
                     /locus_tag="PL014_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOT57417.1"
                     /translation="MNPWPVVALPALASLIGLFTGQDLRDQRLAKGLAVGASAASLVL
                     VVLAWVSGAEPLEAAGPGVAAGELSVPLSLLSDSTRLAVAAVVALVALCVQAYSAVYL
                     RTDDRYARFAATVSLFTAAMLLVVFSGDLVLTVLGWEVMGWCSYLLIGHWSRRHSARR
                     AAHKAFLVTRVADIGFVLGMVGLAAGAGTTSLTGVVDAWSGEGADGAARAVAMTLIVI
                     GVLGKSAQVPFQDWLADAMEGPTPASALIHAATMVAAGTVVLAGLFPVLVLSQPARWV
                     LGISVAVTMVLAAVLAFAQSDLKRLLAWSTVSQVGVMLSALAVAPLAEGPDAGLFHLW
                     AHAIFKALLFLAIGWASVIAGGTAATTLRGSATAIPILRVSFLFGLLSLAGVPVVVGG
                     LSKEHVIAAAWHGAVEPGPGLVVLVALLLTVAVTAAYCTRAYLLLTAHVRSGDEGGPA
                     VEQAGAAAVPPVVQVVLGGLVVLSAIGGLVLFTDAFDVGSASLAWIAITLLIIAVGVA
                     AALRGGLDKDPAQVLAARLVPHADAGLGADALYRAVAVTPVLALARVVAFLDTEVVDL
                     YVRGTAVATRAAGWAGARAHHGERPSSAIGLVLGAVVVLALVGVLAS"
     gene            complement(6374..6682)
                     /gene="nuoK"
                     /locus_tag="PL014_09755"
     CDS             complement(6374..6682)
                     /gene="nuoK"
                     /locus_tag="PL014_09755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOT57418.1"
                     /translation="MIHLAGPYLLASALVGLGVFGIAVRRNAVLVLVGVELVLSGSLV
                     LLVTTQAAAADRWAAGSILPLFVITIAAAEVVVALAVILAAFRQRERIDLDEPSGEAP
                     "
     gene            complement(6679..7188)
                     /locus_tag="PL014_09760"
     CDS             complement(6679..7188)
                     /locus_tag="PL014_09760"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOT57419.1"
                     /translation="MTALDTAFAATALLAVIGGLFAVTTRHVVHAALWLVVALLGVAG
                     SLLVLGAELVALLLVLVYVGAVVVLVLFALMLTRAPIGPDLAHRVGPARTAGAALLGG
                     AVAVLLGSVLLPLATEPVERADVTSTQVAGQLFGTWVWPFELLSVLLLIALAAALAVS
                     RIPREGRAS"
BASE COUNT         1173 a         2551 c         2624 g          763 t
ORIGIN      
        1 cactcaccgc gcaacggggg atgctctcca tcgtcatgct gctccagctc atctgggact
       61 tgcgtcttca cccagatcac gctgctccaa gacggcggcg gcgtcgccaa cgagaccaac
      121 ctgctcggca cgtacgtcga ccatctcggc accggcaagg gcgacttcgg caccgcgagc
      181 gccgtcagca tcctcatcct cgcgctgacg atcgccctgt gctggtacta cgtgcgctcc
      241 ctcacgaagg aggggtcgtg aacccccgct ccccgctcgc ccgcaccggg ctcagcgtgc
      301 tggcgctggc cctcgcggcc atctggggct tccccgtgta ctggatgctc aactcggcgt
      361 tcctgcccaa cgtcgtgctc cagtcgaccg accccacgtt gctgccgttc ggcggatcgt
      421 tcgacaacct gcgcgtcgtc gtcaccgacc cggccttcct cagcgccctc ggcacctcgc
      481 tcgtcgtgac cctgaccacc gtggtcgctc gttcgtgctc gcgctgctgc tcatccagat
      541 gctgcccgcc gaggggctgt tcatcgccca gtacaagatg atgtccgagc tcggcatgct
      601 caaccggggc atcggcctgg ccgtgctcta cacggcggcg gtcgtgccct tcacgatctg
      661 gatgctgcgc ggtttcgtcg ccgggtcgct gccggcatcg ctgccgagct cgccgacctc
      721 ggcatcgggc tcgacggcgc tgaactggtc ctgcacgaac gcagcagacc gccgatcagg
      781 ggttgatgcg gtggccgtac gccatctgct cgagccgggc gatgcgctcg gccatcggcg
      841 ggtgggtcgc ggtgaggcgc gagatgtcct gcgcccggaa cgggttcgcg atcatcatgt
      901 ggctggcgtt gaccaggtcc tgggtcgggg ccagtggcgc gcgggccacg ccgagctcga
      961 gcttgcgcag cgccgacgcc agcgccagcg ggtcgccggt gagctcggcg ccgtcctggt
     1021 ctgcgtcgta ctcacgggtg cgcgagatcg ccagctggac gaccatggcc gcgaacggag
     1081 cgaggatggc gagcgcgaga cccgcgatgg ggttgcggtt ctcgttgtcc gagccgccac
     1141 cgccgaagat catcccccac gacaggaact gggcgaccgt ggagatgaca ccggcgatcg
     1201 ccgcggccac cgagctcgtg aggatgtcgc ggttgtacac gtgcatgagc tcgtgcccga
     1261 gcaccccgcg cagctcacgc tcgtcgagca gcccgaggat gccctgggtg cagcacaccg
     1321 ccgcgttctc ggggttgcgg ccggtggcga aggcgttcgg cgcctgcgtc ggcgagatgt
     1381 agaggcgggg catcggcttg ccggccttgg tcgacagctc gcgcacgatc cggtacatct
     1441 gcggggcctc ggcctcgctg accggttggg cgtgcatcgc cttgatggcg agcttgtcgg
     1501 agttccagta cccgtagaac gtcgtcgcca cgccgatgaa cgcgaagacg aggaggaaga
     1561 cgctgctgcc ggtcatctgc gcgaccaccc agccgatgcc gagcaggatc gcccacatgc
     1621 cgccgaacag cgcggcggtc ttcagtccgt tgtggtgcct gtgcatgttt cctccaacgc
     1681 agcgagcagg ccggttcgtt ccgtcatgac accagaccga gcagcagtcc cgggagggcg
     1741 ctgagcagga cgagcagcac cgttcctgcc acgaggaccg ccctcgcccc accaccggat
     1801 gcgggggtgc tcacctcgtg cgtcgcggcg cccggctcgc tcatctcacc ggcatccccg
     1861 cgtgcgcccg cgtcgccgat cagcaccgcg aaccagcgca ggtagacggc gatgccgagc
     1921 accacggcga cgaccgccac caacgccagc ggccacagcc cctgctccac ggccgggcgc
     1981 agcgcgacga ccttggccac gaggccgatg acgcccggcg gcagcccggc gagcacgagc
     2041 agcgcgaacg cgagcgcacc cccggtgagc cggtcacgcc gcagcagccc acgcatccgt
     2101 gacagcggcc ccgcccccac ggccgcgacg gcggcgaatg ccacgacggt ggccacggca
     2161 tacgtcagca cgtacacggc ggcggcgtgc agcccggcat ctgtcagcgc cgcgaccggc
     2221 agcacgaccc accctgcctg cgcgacggtg gaccacgcga gcaggcgggt cacgtcgtcc
     2281 tggcgcaggg ccatgacgtt gccgagcagc atggatgccg cggcgagcgc gccgatcacg
     2341 atgctcacct gccgcaggtc cccgggcacc ccgacgaagg cctgcacgac gacgaggagg
     2401 gctgccgcag cggagacctt cgaggcagcg gcgagcgcgg tggcggtgcc gaggtcggcg
     2461 gtggagtacg cctgcggggt ccaggcgtgg aacggtacga gcgagagctt gaacccgagc
     2521 ccggcgagca gcacgaccgc cgcgagcacg agcacggcac gccggtgcgg gtccgcccag
     2581 gcgagcgcca cggtgtcggt gccgaacgcg gcatcccctg tggcggtcat ccacagcgcc
     2641 accccgagga cgagcagggc gaacgacgtc accgacgtca cgagcagcgt gagtgcgccg
     2701 tgggcggcgg agcgggtgcc gcgaagcgcc acgagcgcga ccagggggat ggtggcgagc
     2761 tcgagcgtga ccagccaggt gccgaggtcg cgcgacgccg cgacgccggc gccgcccgtc
     2821 gcggcggcga gcagcagggc cgtcgtcacc gtcgtgtcgc gcgggccgcc gtcccggtcg
     2881 tgcagcaggg cgagcgccgc ggccgtcgct gccaggatgc cggcctgcag catgccggtc
     2941 atcgggctcg cggtccacag gcaggctccc tcgggcgccg gcaggcacag cgtgcggacc
     3001 gcaccgtccg cggcctgcac cgccgagatc agggcaccga cggcgccgac gacgagcacg
     3061 gccaggccca ggcccacctg ccagtcgcgg cgccccggca gcagcgcgtc gccgagcagc
     3121 acgagcacga ccccgatggc gggcagcagc gccggggcga gcgcaacggc atcgatgctg
     3181 acgagtgagg cggtgctgat ggctgcggtc atggccacgt cccgatcagc tggctgacgg
     3241 catcggccgt gcggtcgagc acgatccccg ggaagacgcc gaggaggatg acggatgccg
     3301 cgagcacccc gaggatgccg agttcggttc cgtcggcgtc gggggtcacc cgctccccct
     3361 cctggagggc gtcaccgacc cagacggtgc gggccacccg aagcgcatag gcggcagcga
     3421 gcgcagcgcc cacggccgcg acgaccgcgc aggcgatgaa cagggcgcgg gagcgaccgt
     3481 ccgccggcga ccacgcggca tacacggcga agaactcacc ccagaacccg gcgagaccgg
     3541 gcaggcccag gctcgcggcg aggccgagca cgagcaggaa gcccagccgc ggcgacgact
     3601 ctcgaagggc cggtcgcggc accgtgaggt cgacgctgcc ccaccgcgcc ttcagcccgc
     3661 cgacgaggaa gaacagcagg gccgagatga cgccgtgggc gatgttcgcg aacagggccg
     3721 cctgcagacc ggtctcggac ccggtcgcga gggccagcac gacgaagccc atgtgcgcga
     3781 cggaggagta ggcgatgagg cgtttgaggt cacgctcgac gaggcagatc aggccacccc
     3841 agatgatccc gacgacaccg aggaccgcga gcaccggggc gagccgggcg aacccgtcgg
     3901 gcacggtggc caccgggagg cggaccaggc cgtaggtgcc catcttcagc agcacggcgg
     3961 ccagcagcac cgacccggcg gtgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnncggcg tcgtgcgggt cgccgtaccg gctgatgagc acccacatcg gcacgaggac
     4141 gagttcgaag gcgacgaaga acagcacggc atcgcgggcg gtgaacgtcg cgagggcccc
     4201 ggcctcgacg agcagcaggc acccgaggaa cgccgccggg ctgcccccgg ccggcaggtc
     4261 gacccaggcg tgggcggcca cgagcacccc gatggcggcg gtgaggagaa cgagcgggac
     4321 ggcgatgccg tcgaccccga acgtccagcg cacccccaga tcgggcaacc aggcgcggtc
     4381 gacgcttgac ccggcagcgc tgacgacgac cgccagcccc agcgtgagca ctgcggccac
     4441 tgccgtgacg agccgggcca cggccaccga cacgtcgcgg cccgcggtca cgagccacaa
     4501 cccgacggcg gcgggcaggg cgaggagcag actgatcatg aggcgagcac ccccaccagc
     4561 gcgagcacga ccacggcccc gaggacgagc ccgatggccg aactcggccg ctccccgtgg
     4621 tgggccctgg ccccggccca ccccgcggcc cgggtggcga cggccgtgcc gcgcacgtag
     4681 aggtcgacga cctcggtgtc gaggaacgcg acgacgcggg cgagcgcgag gaccggcgtg
     4741 acggcgacgg cgcggtagag cgcgtcggcg cccagcccgg catccgcgtg gggaaccagg
     4801 cgggccgcga gcacctgcgc cgggtccttg tcgaggccgc cacgcagggc cgcagcgaca
     4861 ccgacggcga tgatgagcag ggtgatcgcg atccaggcga gcgaggccga cccgacgtcg
     4921 aaggcatcgg tgaacaggac gaggccgccg atcgcgctga gcacgacgag gccaccgagc
     4981 accacctgga cgacgggtgg caccgcagcc gctccagcct gctccaccgc cggacccccc
     5041 tcgtcgccgc tgcggacgtg tgcggtgagc aggaggtagg cacgggtgca gtaggcggcg
     5101 gtgacggcca ccgtgagcag cagggcgacg aggacgacca gacccggccc gggttcgacg
     5161 gcaccgtgcc acgccgcggc gatgacgtgc tccttggaca ggccaccgac gacgacgggt
     5221 actccggcga gcgacaacag cccgaagagg aaagagaccc gcaggatcgg gatcgcggtg
     5281 gcgctgccgc gcagggtggt ggccgccgtg ccgcccgcga tgaccgacgc ccacccgatc
     5341 gcgaggaaga gcagcgcctt gaagatggcg tgcgcccaca ggtggaacag cccggcatcc
     5401 ggcccctcgg ccagcggcgc gacggccagg gccgagagca tgactcccac ctggctgacc
     5461 gtcgaccacg cgagcaggcg tttgaggtca ctctgggcga aggcgagcac cgcggcgagc
     5521 accatcgtca cggcgaccga gatgccgagc acccagcggg ccggctggct gaggacgagg
     5581 accgggaaga gcccggcgag caccacggtg ccggccgcca ccatcgtcgc cgcgtggatg
     5641 agcgcactgg cgggggtcgg gccctccatg gcatcggcga gccagtcctg gaagggcacc
     5701 tgcgccgact tgccgagcac gccgatgacg atgagcgtca tggcgacggc gcgcgcggcg
     5761 ccatcggcgc cctcgccgga ccaggcgtcg acgacgccgg tcaggctggt cgtgccggct
     5821 ccggcggcga ggccgaccat gccgaggacg aacccgatgt cggcgacgcg ggtgaccagg
     5881 aaggccttgt gagcagcccg gcgcgcgctg tgtcgacgcg accagtgccc gatgagcagg
     5941 taggagcacc agcccatgac ctcccacccg agcaccgtca gcacgaggtc gccggagaac
     6001 acgacgagga gcatggccgc ggtgaacagc gacacggtgg cggcgaaccg ggcgtagcgg
     6061 tcgtcggtgc gcaggtagac cgccgagtag gcctgcacgc acagtgcgac gagcgcgacg
     6121 acggccgcga cggccaggcg ggtcgagtcg ctcagcagac tcagcggcac gctcagctca
     6181 ccggcggcaa cccccgggcc ggccgcctcg aggggctcgg cacccgagac ccaggccagg
     6241 acgacgagca cgagggatgc cgcgctcgca cccacggcga gccccttggc cagccgttgg
     6301 tcgcgaaggt cctgaccggt gaacagcccg atcagcgacg cgagggcggg cagcgccacc
     6361 accggccacg ggttcacggg gcctccccgc tcggctcgtc gaggtcgatg cgctcacgct
     6421 gccggaacgc cgcgaggatg accgcgaggg cgacgacgac ctcggccgcc gcgatggtga
     6481 tgacgaacag cggcaggatg ctgcccgccg cccaccggtc ggccgcggct gcctgggtcg
     6541 tgacgagcag gacgagcgag ccgctgagca ccagctcgac cccgacgagc acgagcacgg
     6601 cgttgcggcg cacggcgatg ccgaagacgc cgagcccgac gagggcgctg gcgagcaggt
     6661 agggcccggc gaggtggatc acgacgcccg tccctcacgc gggatgcgcg agacggccag
     6721 ggccgcggcg agcgcgatga gcagcagcac ggacagcagt tcgaagggcc agacccacgt
     6781 gccgaagagc tgcccggcga cctgggtgct cgtgacgtcg gcccgctcga ccggctcggt
     6841 ggccaggggc agcagcaccg agccgagcag caccgcgacg gcgccgccga gcagcgcggc
     6901 cccggcggtg cgcgccgggc cgacgcggtg ggcgaggtcg ggtccgatcg gcgcccgcgt
     6961 gagcatcagc gcgaagagca cgagcacgac gacggcgccc acgtagacga gcacgagcaa
     7021 cagggcgacg agctctgccc cgagcacgag cagcgacccg gcgacgccga gcagggcgac
     7081 gaccagccag agcgccgcgt gcacgacgtg gcgggtggtg acggcgaaca ggccaccgat
     7141 gacggcgagg agggcggtcg cggcgaaggc cgtgtccagg gccgtcacgg ggaggcctcg
     7201 ccggcatccg g
//
LOCUS       DAOSOC010000129         7166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_10473_length_7166_cov_9.988890, whole genome shotgun sequence.
ACCESSION   DAOSOC010000129 DAOSOC010000000
VERSION     DAOSOC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10473_length_7166_cov_9.988890"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1586
                     /locus_tag="PL014_09765"
     CDS             258..1586
                     /locus_tag="PL014_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOT57420.1"
                     /translation="MQQVIIEVDPHKLSATIEVVDDHERRLGEGRFTTDRAGYAAMRT
                     YVKAWPQRVWAVEGANGTGRPVAQRLLESGEDVLDVPAKLAARVRLFDTGHNRKTDAH
                     DAHSIAMVAVRTQGLRVLHVDGELQALRMLVDRREALSRRRVQTVDRLQALLAELLPG
                     QAKKDITTGQAKTMLATVRPRDLAGKTRRRIAAEELAELVAVEAKQKKATAELKAMVT
                     AGGSHLMDLYGVGPVVAARVLADVGDVARFADRNRFASWNGTAPLDASSGENNRHRLS
                     RAGNRRVNHMIHIAAVTQLRLPTDGRVYYRRKRAEGKKPMEAMRCLKRRISDAIYRQL
                     VADAMATTPRVNAGPGGHCGASLVSSAAGFNPHTGTSDQPLPGPAIPTLPANTVLANP
                     PAGAGSPAQAPTCRSRQGGAPHRTNDLDGDKRRRTLQRADPGPLTTEGRR"
     assembly_gap    1718..1817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1818..2461)
                     /locus_tag="PL014_09770"
     CDS             complement(<1818..2461)
                     /locus_tag="PL014_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOT57421.1"
                     /translation="MDLDAYLMVHQPQWKRLEELSGRRRLTGAEADELLDLYQRVSTH
                     LSVVRSASPDPSVVSYLSSVLARARVASAGARTSTWSDLLAFFTHTFPAALYRTRWWW
                     LTTTLASVALAAFVAWWAIDNPQVWSNQLTPAEIEAYVGTDFENYYSEYPHAEFGTLV
                     WFNNARVAALCIALGVLGLPVLYVLGSNVVSVGVAAALMISNDRGALFFGLILP"
     gene            2516..>3304
                     /locus_tag="PL014_09775"
     CDS             2516..>3304
                     /locus_tag="PL014_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT57422.1"
                     /translation="MSDPGSYRSYAGDDMITGEGVAVEVPVASVAARMASGAIDVFVT
                     LVVLIAGVFAIGFVAGGVSDAVIGILFIGLSVLAFIVLPTAVETLTRGRSLGRMALGL
                     RTLRDDGGPTTFRHALVRGLVGYVEIYLLLGVPAVIASMVHPRAKRLGDMAAGTIVIS
                     QRAALRLTPPPPMPPALMPWALSADIAALPSGLTVAIRQFLARAPGLSPASRHTLGVD
                     LLASTLAYVAPQPPPGFHPEVVLSAIVAERRRRDLDRLWREEQLR"
     assembly_gap    3305..3404
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3405..4448)
                     /locus_tag="PL014_09780"
     CDS             complement(<3405..4448)
                     /locus_tag="PL014_09780"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOT57423.1"
                     /translation="MSSAVALTPSIVARAPKALLHDHLDGGLRPQTIVDLAAESGYDG
                     LPTTDAVELGQWFLDSADSGSLVRYLETFSHTVAVMQTREQLVRVARECALDLAADGV
                     VYAESRFAPEQHLAGGLSLEHVVEAVLEGFAQGQAEAAEAGTPIRVVTLLTAMRHAAR
                     SREIAELAVRYRDEGVGGFDIAGAEAGFPPTRHLDAFEYLRRENAHFTIHAGEAFGLP
                     SIWEAIQWCGADRLGHGVRIVDDIETGADGIPVLGRLAAYVRDMRIPLEMCPSSNLQT
                     GAAESIAAHPITMLKNLRFRVTVNTDNRLMSGTSMTREMQLLVDEAGWTIEDLRWATI
                     NAMKSAFLPFDERL"
     gene            complement(4448..5737)
                     /locus_tag="PL014_09785"
     CDS             complement(4448..5737)
                     /locus_tag="PL014_09785"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOT57424.1"
                     /translation="MTESFDAVDVITTKRDRHELSDDQIRWVIDAYTRGVVADEQMSS
                     LAMAVLLNGMTRREIATWTAAMIASGERMDFSGLSRPTADKHSTGGVGDKITLPLAPL
                     VAVFGVAVPQLSGRGLGHTGGTLDKLESVAGWQAALSNEAMMRQLEDVGAVICAAGSG
                     LAPADKKLYALRDVTGTVEAIPLIASSIMSKKIAEGTGALVLDVKVGSGAFMKNREDA
                     TELARTMVDLGTDAGVNTVALLTDMSVPLGLTAGNALEVRESVEVLAGGGPADVVELT
                     VRLAEEMLDAAGRGGQDVAAALKDGRAMDAWKAMISAQGGDPDAPLPTAKETQEVRAT
                     ASGVLSGLDAYAVGVAAWRLGAGRARKEDPVQAGAGIEIHAKPGDEVREGDVLLTLHT
                     DTPERFERAVAALDGGITIAPAGTSVERSPIVIDRIA"
     assembly_gap    5761..5860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5861..6249)
                     /locus_tag="PL014_09790"
     CDS             complement(<5861..6249)
                     /locus_tag="PL014_09790"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOT57425.1"
                     /translation="MSTADVDWDALHAEAVRMTTLAYAPYSEYRVGVAGFVEDGRMVS
                     GCNVENAGYGVTLCAECGMVSQLHATGGGLLTAVYCVDGAGQALMPCGRCRQLLWENG
                     TPQTLLMTPLGVLPMEQILPQAFGPEHL"
     gene            complement(6246..>7166)
                     /locus_tag="PL014_09795"
     CDS             complement(6246..>7166)
                     /locus_tag="PL014_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT57426.1"
                     /translation="GVCHACGHDVHVTALIGAMLALASCEPALTERGVAVRGIFQAAE
                     EMIPGGAHDAILAGALDGVDAVFAVHCDPSLDVGQVGLREGPITAACDQVAVSLHGRG
                     GHTSRPQLTEDLTYALAKVVTDVPAALSRRLDPRAGAALVWGTIAAGRAPNVIPSTGE
                     CTGTLRMLDAHAWLTVGPLLEEIVHGVVAPYGVQAKVERVKGVPPVVNTSDGIQSFRL
                     AALAGGMVPVSTTQSLGGEDFSWYLAQAPGAMARLGTRTHGGPTFDLHQGDLVVDEDA
                     IALGARLLASVAIVWHTDATRRALDPMETE"
BASE COUNT         1023 a         2440 c         2447 g          956 t
ORIGIN      
        1 tgggcgaagt ggggcgccct cagtgggcta ctagatcatt gtcggtaggg ccggtgtctg
       61 gccagactcg gtccaggtgc tcggcggtga cgctctccag tgggctgccc accagctgca
      121 tcggtgtggc tcatcagcgg tttgctgccc gccgggcacc gtgacgaggc gtggctcaag
      181 aagggactgc cctgctcgta gtagcactgc ccacctcgcc gaccacatcg gaaacgagga
      241 caggcgggag caagagcatg caacaggtca tcatcgaggt tgatccccac aagctgtcag
      301 cgacgatcga ggtcgtcgac gatcacgaac ggcgcctggg cgagggccgg ttcaccaccg
      361 accgggccgg gtacgcggcg atgcgcacct atgtgaaggc gtggccgcag cgggtgtggg
      421 cggtcgaggg cgcgaacggc accggacgac ccgtggcgca gcgactcctg gaatcgggtg
      481 aggacgtgct tgacgttccg gccaagctcg ccgcccgggt ccggctcttc gacaccgggc
      541 acaaccgcaa gaccgacgcc cacgacgcgc actccatcgc gatggtcgcg gtacgcaccc
      601 aaggcctgcg ggtgctgcac gtcgacggcg aactgcaggc gttgcggatg ctggtggacc
      661 ggcgtgaggc gctgagccgg cgccgggtcc agaccgtcga ccggttgcag gccctactgg
      721 ctgagctgct gcccgggcag gcgaagaagg acatcaccac cggccaggcc aagacgatgc
      781 tcgccacggt gcgcccgcgc gacctcgccg ggaagacccg acgccggatc gccgccgagg
      841 agctcgccga gctcgtcgcc gtcgaggcca agcagaagaa ggccaccgcc gagctcaaag
      901 cgatggtcac cgccggcggc tcacacttga tggacctata cggcgtcggc ccggtcgtcg
      961 ccgccagggt cctggccgac gtcggcgacg tcgcccgctt cgccgaccgc aaccgcttcg
     1021 cgtcgtggaa cgggaccgcc ccgttggacg cgtcctccgg ggagaacaac aggcaccggc
     1081 tgtcccgggc cggcaaccgg cgggtcaacc acatgatcca catcgctgcg gtcacccagc
     1141 tccgcctgcc caccgacggt cgggtctact accggcgcaa gcgcgccgag ggcaagaagc
     1201 cgatggaggc gatgcggtgc ctgaagcgcc gcatctccga cgcgatctac cgacagctgg
     1261 tcgccgacgc catggccacc acgccaaggg tgaacgcggg cccgggaggg cactgcgggg
     1321 cgtcacttgt atccagcgcg gccggcttca acccgcacac cggcacttcg gatcagccac
     1381 ttcccggacc cgcaataccg acgctacccg cgaacacggt ccttgcgaac ccccctgccg
     1441 gggccggctc cccagcccag gcgccgacgt gccggagccg tcaaggtgga gcgccccacc
     1501 ggacgaacga ccttgacggc gacaagcgcc ggcgcacact ccaaagagcc gatcccggcc
     1561 ccttgacgac agaagggcgc cgatgagacg cggtccttcg atgcgagaat tgtcggctgg
     1621 ggcggatgcc ggtcaggcgg cggtgacggg ggcgacgtca ccagcatcgc gcgccatcac
     1681 gtcacctgtg gccccctgcg caacggcggc ccgcccgnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnntgc ggcaggatca ggccgaagaa cagggcaccg cggtcgttgc
     1861 tgatcatcag ggcggcagcc accccgacag agacgacgtt cgagcccagc acgtagagca
     1921 cgggcagccc gagcaccccg agcgcgatgc acagtgccgc gacccgggcg ttgttgaacc
     1981 acacgagcgt gccgaactcg gcgtgcgggt actcggagta gtagttctcg aagtcggtgc
     2041 cgacgtacgc ctcgatctcc gcaggcgtca gctgattgct ccacacctgc gggttgtcga
     2101 tggcccacca cgccacgaac gcggccagcg cgacgcttgc cagggtcgtg gtgagccacc
     2161 accagcgcgt gcggtacagc gccgccggga acgtgtgggt gaagaacgcc aacaggtccg
     2221 accaggtcga ggtgcgcgct ccggccgagg cgacccgggc ccgggccagc acggaggaca
     2281 ggtagctgac gaccgacggg tcggggctgg cggaccgcac gaccgacagg tgggtcgaca
     2341 cccgctggta gaggtcgagc agctcatcgg cctcggcccc ggtgagacgc cggcgaccgg
     2401 acagctcctc gaggcgcttc cactggggct ggtgcaccat gaggtaggcg tcgagatcca
     2461 cccgtgaaga ctaactgccg gggcccggcg gcgccggtcg ggttagcatc ggcaggtgag
     2521 cgatccgggg agctaccgca gctacgccgg ggacgacatg atcaccggcg aaggcgtggc
     2581 cgtcgaggtg ccggtcgcga gcgtcgccgc ccgcatggcg tccggggcca tcgacgtctt
     2641 cgtcacgctc gtggtcctca tcgccggggt gttcgcgatc ggtttcgtcg cgggcggtgt
     2701 gtcggatgcc gtgatcggga tcctgttcat cgggctcagc gtcctggcct tcatcgtcct
     2761 cccgacggcg gtcgagaccc tgacacgtgg acgcagcctc gggcggatgg ctctcgggtt
     2821 gcgcaccctg cgcgacgacg gcggcccgac gaccttccgg cacgccctcg tccgtggcct
     2881 cgtgggctac gtcgagatct acctgttgtt gggtgtccca gccgtcatcg cgtcgatggt
     2941 gcacccgcgc gccaagcggc tcggcgacat ggctgccggc accatcgtca tctcccagcg
     3001 ggcggcgctg cgcctgaccc ctcccccgcc gatgccgccg gccctgatgc cctgggccct
     3061 gtcggcagac atcgccgccc tgccctccgg cctgaccgtc gccattcggc agttcctggc
     3121 ccgggcaccg ggtctgtcac cggcctcgcg ccacaccctc ggggtcgacc tgttggcctc
     3181 gaccctggcc tacgtcgcgc cccaaccgcc gcccgggttc catcccgagg tggtcctgtc
     3241 ggccatcgtc gccgaacggc gtcgccgcga cctcgaccgc ctctggcgcg aggagcagct
     3301 gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncagccg ctcgtcgaac
     3421 ggcaggaacg ccgacttcat cgcgttgatc gtcgcccagc gcaggtcctc gatggtccaa
     3481 ccggcctcgt cgacgagcag ctgcatctcg cgggtcatcg acgtgccgct catgagccgg
     3541 ttgtcggtgt tcaccgtgac ccggaatcgc aggttcttca gcatcgtgat cgggtgcgcc
     3601 gcgatcgact cggcggcccc ggtctgcagg ttcgagctgg gacacatctc gagcgggatg
     3661 cgcatgtcgc gcacgtaggc ggccaaccga ccgagaacgg ggatgccgtc cgcccccgtc
     3721 tcgatgtcgt cgacgatccg cacgccgtgc ccgagccggt ctgcgccaca ccactggatg
     3781 gcctcccaga tcgacggcag cccgaaggcc tcaccggcat ggatcgtgaa gtgcgcgttc
     3841 tcgcggcgca ggtactcgaa ggcgtcgagg tggcgggtgg gagggaaccc ggcctcggcc
     3901 ccggcgatgt cgaagccgcc gacgccctcg tcgcggtagc gcacggccag ctcggcgatc
     3961 tcccggctgc gggcggcgtg ccgcatcgcc gtgagcagcg tgacgacccg gatcggcgtc
     4021 ccagcctccg cggcctcggc ctgcccctgc gcgaacccct cgagcaccgc ctcgacgacg
     4081 tgctcgagcg agagcccgcc ggccaggtgc tgctcgggcg cgaaccttga ctccgcgtag
     4141 acgacaccgt cagcggcgag gtcgagcgcg cactcgcgag cgacccgcac gagctgctca
     4201 cgcgtctgca tcaccgccac ggtgtgcgag aacgtctcga ggtatcgcac cagggagcca
     4261 gagtccgcgg agtccaggaa ccactgcccc agctcgacgg catccgtcgt cggcaacccg
     4321 tcgtagccgc tctccgcggc caggtcgacg atggtctgcg gccgcagccc accgtcgagg
     4381 tggtcgtgca gcagcgcctt gggggcgcgg gcgacgatgg agggggtcag ggcgaccgcg
     4441 gaggacatca ggcgatcctg tcgatgacga tgggcgagcg ctcgaccgag gtgccggccg
     4501 gcgcgatggt gatgccgccg tcgagggcgg cgacggcgcg ctcgaagcgc tcgggggtgt
     4561 cggtgtgcag cgtgagcagc acgtcgccct cgcggacctc gtcgccgggc ttggcgtgga
     4621 tctcgatgcc ggccccggcc tgcaccgggt cctccttgcg ggcccggccc gcaccgaggc
     4681 gccacgcggc gacacccacg gcgtacgcgt cgaggccgga cagcaccccg gatgccgtgg
     4741 cccgcacctc ctgcgtctcc ttggccgtcg gcagcggggc gtcggggtca ccaccctggg
     4801 cggagatcat cgccttccag gcgtccatcg cgcggccgtc cttcagcgcg gcggcgacgt
     4861 cctgaccgcc gcgacccgcg gcatccagca tctcctccgc caggcgcacg gtgagctcga
     4921 cgacgtcggc gggcccgcca ccggcgagca cctcgaccga ctcgcggacc tcgagcgcgt
     4981 tgccggccgt gaggcccagg ggcactgaca tgtcggtgag gagcgcgacg gtgttcacgc
     5041 cggcatccgt gccgaggtcg accatcgtgc gggcgagctc ggtggcgtcc tcgcggttct
     5101 tcatgaacgc gccgctgccg accttgacgt cgagcacgag cgcgccggtg ccctcggcga
     5161 tcttcttgct catgatcgag ctcgcgatga gcgggatcgc ctcgacggtg ccggtgacgt
     5221 cacgcagcgc gtacagcttc ttgtcggcgg gtgccaggcc cgacccggcg gcgcagatga
     5281 ccgcaccgac gtcctcgagc tggcgcatca tcgcctcgtt ggacagggct gcctgccagc
     5341 cggcgaccga ctcgagcttg tcgagcgtgc cgccggtgtg gccgaggccg cgccccgaca
     5401 gctgcggcac ggcgacgccg aacacggcga ccagcggtgc cagcggcagg gtgatcttgt
     5461 cgccgacgcc gcccgtggag tgcttgtcag cggtcgggcg cgacaggccg gagaagtcca
     5521 tccgttcgcc gctcgcgatc atcgccgcgg tccaggtcgc gatctcgcgg cgggtcatcc
     5581 cgttgagcag gacggccatg gccagcgagc tcatctgctc gtcggccacc acgccgcgcg
     5641 tgtacgcgtc gatgacccag cggatctggt cgtcggacag ctcgtggcgg tcccgcttgg
     5701 tggtgatgac gtcgacggca tcgaaggatt cagtcatcgg cccatcatgt cagggccggg
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aggtgctcgg ggccgaacgc
     5881 ctggggcagg atctgctcca tcggcagcac tccgagcggg gtcatgagca gggtctgcgg
     5941 ggtgccgttc tcccagagca gctggcggca gcggccgcac ggcatcaggg cctggccggc
     6001 accgtcgacg cagtagaccg cggtcagcag gccgcccccg gtggcgtgca gctgcgacac
     6061 catgccgcac tcggcgcaca gggtcacccc gtagccggcg ttctcgacgt tgcacccgga
     6121 gaccatccgg ccgtcctcga cgaaaccggc gacaccgacg cggtactcgc tgtacggcgc
     6181 atacgccagg gtcgtcatcc gcacggcctc ggcgtgcagg gcgtcccagt cgacgtcggc
     6241 ggtgctcatt cggtctccat cgggtcgagg gcgcgacggg tcgcgtcggt gtgccagacg
     6301 atcgccaccg atgcgaggag gcgggcaccg agggcgatgg cgtcctcgtc gacgacgagg
     6361 tcaccctggt gcaggtcgaa cgtggggcct ccgtgggtgc gggttcccag acgggccatc
     6421 gcgccgggag cctgcgcgag gtaccaggag aagtcctcac cgccgagcga ctgggtcgtg
     6481 gagacgggga ccatgccgcc ggcgagggca gcgaggcgga acgactggat gccgtcgctg
     6541 gtgttgacga cgggcggcac acccttgacc cgctccacct tggcctgcac cccgtacggg
     6601 gcgacgacgc cgtggacgat ctcctcgagc agcgggccga cggtgagcca ggcgtgggca
     6661 tcgagcatgc gcagcgtgcc ggtgcactca cccgtcgacg ggatgacgtt gggggcgcga
     6721 ccggcggcga tggtgcccca caccagagcg gctccggcgc gggggtccag ccggcgcgag
     6781 agcgctgcag gcacgtcggt gacgaccttg gccaaggcgt aggtgaggtc ctcggtcagc
     6841 tgcgggcggg aggtgtgccc gccgcggccg tgcagggaga ccgccacctg gtcgcaggcc
     6901 gcggtgatcg gcccctcacg caggcccacc tgccccacgt cgaggctggg gtcgcagtgc
     6961 accgcgaaga ccgcgtcgac cccgtcgagc gctccggcga ggatggcgtc gtgggccccg
     7021 ccggggatca tctcctcggc cgcctggaag atgccgcgaa ccgcgacacc gcgctcggtc
     7081 agcgccggct cgcaggaggc cagcgccagc atcgccccga tgagcgccgt gacgtgcacg
     7141 tcgtgcccgc aggcgtggca cacccc
//
LOCUS       DAOSOC010000130         7114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_10573_length_7114_cov_8.653634, whole genome shotgun sequence.
ACCESSION   DAOSOC010000130 DAOSOC010000000
VERSION     DAOSOC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10573_length_7114_cov_8.653634"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PL014_09800"
     CDS             complement(<1..389)
                     /locus_tag="PL014_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57427.1"
                     /translation="MARHTSSIRPFAVTTAVIAVVLAGGLASTAQSALAAGSTPDPSR
                     FTATPITAVESHFDVPKSASGKAAQSDPALLRRTDDAVVGVMVKVDIDPVASYAGGIK
                     GLKATSPAATKKPLKANKAAVAAYTKHA"
     gene            complement(617..1762)
                     /locus_tag="PL014_09805"
     CDS             complement(617..1762)
                     /locus_tag="PL014_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT57428.1"
                     /translation="MAGAAVGATTAVASGAGSLAAAAYFARRVLTPDRQRPDDVVVRA
                     AGAHTVTLTATPQTVVPGRYGLWIDNGFGHVRIGEVVDLDEGSATVERELIAVDRGYL
                     APGTARWNAYFHGAPPDVTIGAHTEHVTYAGELGPMPAWVVRPDGADSPPDHRWAVLV
                     HGRGARREETIRAMPPLLEQGWTCLVPSYRNDEGVPSGPDGRYALGLSEWRDIEAAID
                     HAVDHGAQEVLLAGWSMGGAIVLQLLDRSPLSELVSRVVLDAPVIDWGDVLAHHAHSH
                     RLPRHVGSLARTMMNQRWGHRLVGVGESVDLAKTDWVSRAHELHHGILLIHSAADDFV
                     PIGPSRALAQARPDLVQFHEWEVAHHCTEWNTEPERWERLVGEFAAP"
     gene            complement(1825..3987)
                     /locus_tag="PL014_09810"
     CDS             complement(1825..3987)
                     /locus_tag="PL014_09810"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOT57429.1"
                     /translation="MVDAQVLEIEGPNGPRQVRLSSPDRLMWPGAGITKGDLAAYVMA
                     VGDSLVHHIGDRPVTLQRFPDGIEGEEFYQKNPPKGLPEWVRTVSCRYPSGRRHAQIV
                     IDEVASAVWAVQMNTVTYHPWPVRTANNDNPDELRIDLDPQPGRTFADAVEAAVALRE
                     LLTEVGLTGWAKTSGNRGVHVYARIGPTHEFLDVRHGVIGIARELERRLPDLVTSSWW
                     KEERGERVFVDFNQACRDRTIASPYSPRPLPGAPVSMPVTWAELETVSPGDFTVLTVP
                     DVLEDRGDAWADIDGAVGDVSGALALWQRDVDERGLGELNFPPDYPKMPGEPPRVQPS
                     KRRTDKADDEYMPPKAERDAAERERWGMPVVPPVPPMLAKAVKGIAALDGKDVSFEPK
                     WDGFRSIIFRSGDLVEIGSRNEKPMTRYFPEVVEAALANLPERCVVDGEIIVVRPGED
                     RLDFDLLSQRIHPAASRVAKLSVETPARFVAFDLLALGDDDLTGRPFSERRALLEQAL
                     AAASAPIHLTPATTDRALAAQWFTEFEGAGLDGLVAKTLDGPYEPDKRTMAKIKHERT
                     ADCVVAGYRTHKSGDDLVGSLLLGIHDDAGQLLSVGVIGAFPMARRKELFDELQPLVT
                     TFEDHPWNWAAHEEGNRTPRAAAGSRWAAGKDLSFTPLRPELVVEVRYDHMEGVRFRH
                     TAQFARWRPDKSPADCTYDQLDEPVSYDLSEVLAEGGS"
     gene            4158..4601
                     /gene="msrB"
                     /locus_tag="PL014_09815"
     CDS             4158..4601
                     /gene="msrB"
                     /locus_tag="PL014_09815"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOT57430.1"
                     /translation="MTMKPSDRSYAVERTEEEWKAALSPAEYQVLRQAGTERPFVGEY
                     TDAKTVGTYACRACGAELFTSETKFDSHCGWPSFFSPLAGESVELLEDRSLPGRPRTE
                     VRCATCGSHLGHVFEGEGYDTPTDQRYCINSISLTLHPKDEPAAG"
     gene            4793..5725
                     /locus_tag="PL014_09820"
     CDS             4793..5725
                     /locus_tag="PL014_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOT57431.1"
                     /translation="MTTTVDPALRALAAGQHGMFTLAQARTLGVSPLVLERLVRENRL
                     LHPGRGLYAVTEDVDQAPEPWHRHLCVGAHLLYRDASLTSASAALAHGLPVWGVDLSR
                     PDLHRPVDRAVGVKAFRVRPRPKNPLRPEPVTTELGRADEVATSVVQLALDHGPVAGT
                     VSADNAVHRGLVTIDALTTAGDLVASWPGSSRVRSMLAFVDGRSESVGETRCRIELVT
                     HGIPLVPQVPIRDRDGNVFARVDFVIEGYKVVVEFDGRVKYAEGDKQVLWDEKRREDR
                     LRALGYLVVRITWADLEHPGAAVAKVRRAMAQAA"
     gene            complement(5859..6584)
                     /locus_tag="PL014_09825"
     CDS             complement(5859..6584)
                     /locus_tag="PL014_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HOT57432.1"
                     /translation="MSEPARPVPGNPNAARIREINDTIRYAMWSVFAVAEPIGDEDRD
                     ALAAEVEELFTDLAAKDVVVRGTYDVAGLRADADVMVWWHAETIEALQEAYHALLRTA
                     YGQHLDPVWSVAALHRPAEFNKSHIPAFMADEVARDYVCVYPFVRSYDWYLLEDAERR
                     DLLREHGQMARDYPDVRANTIASFALNDYEWILAFEADELHRIVDLMRELRASRARMH
                     VREEVPFYTGPRVDVRTLVARQR"
     gene            complement(6581..>7114)
                     /locus_tag="PL014_09830"
     CDS             complement(6581..>7114)
                     /locus_tag="PL014_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HOT57433.1"
                     /translation="ETASSAVVTLAVERRDLAEVRGSGFLVPPVEGRAIKASTFSFRK
                     WAWTGEVSDDVVHLRASLGRAREEAVLQRDDADLVAVSVAEVGEALGRPLPRVVDAHV
                     QRWGGGLPQYAVGHVDAVASVRADVARLPGLEVAGAAYDGVGIPAVIASATRAARAAL
                     SHLSTIPSTPHQGEHRA"
BASE COUNT         1023 a         2467 c         2543 g         1081 t
ORIGIN      
        1 gcgtgcttgg tgtaggccgc gacagccgcc ttgttcgcct tgaggggctt cttggtggcg
       61 gcggggctgg tcgccttcag ccccttgatc ccgcccgcgt acgacgcgac ggggtcgatg
      121 tcgaccttga ccatcacgcc caccacggcg tcatccgtgc gcctgagcag cgccgggtcg
      181 ctctgggcgg ccttgccgct tgccgacttc gggacgtcga agtgggactc gaccgccgtg
      241 atcggggtcg ctgtgaatct cgaggggtcg ggcgtggagc ccgctgccag cgccgactgg
      301 gcggtgctgg ccagacctcc cgcgagcacg acggcgatga ccgcggtggt caccgcgaac
      361 ggacggatgg acgaggtgtg tcgtgccacg tgcttcctcc gggcggacga gccccgaggg
      421 ggcgaccccg aggcacctgt ggggccccag cgggcaccct cgcgcacgct atacctgtgg
      481 cccggggcgg gacatagccc gaaatgacta tgagcccacc taacgcaact tgtcccccga
      541 aggtgacccc ggccgcgtgc tcgacgcgac tgcagacatg ctcgacaggg ctggattcag
      601 accgtggtgg tctgcctcac ggggcggcga actctcccac gaggcgctcc caccgctcgg
      661 gttcggtgtt ccactcggtg cagtggtggg ccacctccca ctcgtggaac tgcacgaggt
      721 cagggcgcgc ctgcgccagg gctcgcgacg gtccgatcgg cacgaagtcg tcagcggcgg
      781 aatggatgag caggatgccg tggtgcagtt cgtgggcccg gctcacccag tcggtcttgg
      841 cgaggtcgac gctctcgccg actccgacga gccggtgtcc ccagcgctgg ttcatcatgg
      901 tgcgcgcgag cgagccgacg tgccgcggca gtcggtggga gtgcgcgtgg tgggccagga
      961 cgtcacccca gtcgatgacc ggggcgtcca ggaccacccg ggacacgagc tcggagagcg
     1021 gtgagcggtc gagcagttgc aggacgatcg ccccacccat cgaccacccc gccagcagga
     1081 cctcctgggc gccgtggtcc accgcgtggt cgatggccgc ctcgatgtcg cgccactccg
     1141 acagtccgag cgcgtagcga ccatcgggcc ccgacggcac tccttcgtcg ttgcggtagc
     1201 tcgggaccag gcaggtccag ccctgctcga ggagcggggg catggcccgg atcgtctcct
     1261 cgcgccgggc tccccggccg tgcacgagca ccgcccaccg atgatctggc gggctgtcag
     1321 caccgtcggg ccgcaccacc caggcgggca tcggacccag ctcaccggca tacgtgacgt
     1381 gttctgtgtg cgcaccgatg gtgacgtcgg gaggtgcgcc gtggaagtac gcgttccacc
     1441 gggcggtgcc gggggcgagg tacccacggt cgacggcgat cagctcgcgc tcgacggtgg
     1501 ccgagccctc gtcgaggtcg accacttctc cgatgcgcac gtggccgaag ccgttgtcga
     1561 tccacaggcc gtagcgtccc gggacaaccg tctggggggt cgcggtcagc gtgacggtgt
     1621 gtgccccggc ggcgcgcacg acgacgtcgt cagggcgctg tcggtcaggg gtcagcacgc
     1681 gacgcgcgaa gtacgcagcg gcggcgagcg acccggcgcc gctcgcgacg gccgtcgtcg
     1741 ccccgactgc cgcaccggcc accgcggcag tgcgtacccg accactggtg ggcgtcgacg
     1801 tcgacgacga cctcgtcgac tgcgtcatga cccgccctcc gcgagcacct cggacaggtc
     1861 gtaggacacc ggctcgtcga gctggtcgta ggtgcagtcg gccgggctct tgtcgggacg
     1921 ccagcgggcg aactgcgcgg tgtggcggaa ccgcacgccc tccatgtggt cgtagcggac
     1981 ctcgacgacg agctcggggc gcaggggcgt gaacgacagg tccttgccgg cggcccaccg
     2041 gctccctgcc gcagcccgcg gggtgcggtt cccctcctcg tgcgccgccc agttccacgg
     2101 gtggtcctcg aaggtcgtga cgagcggctg caactcatcg aagagctcct tgcggcgggc
     2161 catggggaac gccccgatga cccccaccga gagcagctgc ccggcgtcgt cgtggatgcc
     2221 gagcaacagg ctgcccacga ggtcgtcacc gctcttgtgg gtgcggtacc ccgcgacgac
     2281 gcagtcggca gtgcgttcgt gcttgatctt ggccatcgtg cgcttgtcgg gctcgtacgg
     2341 cccgtcgagg gtcttggcca ccagcccgtc gagaccagcc ccctcgaact cggtgaacca
     2401 ctgggcagcc agggcccggt cagtcgtcgc cggggtgagg tggatggggg cgctcgcagc
     2461 ggcgagagcc tgctccagca gggcccgccg ctcgctgaac ggtcgcccgg tgaggtcgtc
     2521 gtccccgagc gccagcaggt cgaacgcgac gaaccgggcg ggggtctcga ccgacagctt
     2581 ggcgaccctg ctggcagccg ggtggatgcg ctggctcagc aggtcgaagt cgagacggtc
     2641 ctcccccggt cgcacgacga tgatctcgcc gtcgacgacg cagcgttcgg gcaggttggc
     2701 cagagcggcc tcgacgacct cggggaagta gcgcgtcatc ggcttctcgt tgcggctgcc
     2761 gatctcgacg aggtcgcccg agcggaagat gatcgagcgg aagccgtccc acttgggctc
     2821 gaagctgacg tccttgccgt ccagcgcggc gatgcccttg accgccttgg cgagcatcgg
     2881 cggcacgggc gggaccaccg gcatccccca gcgctcgcgc tcggccgcgt cgcgttcggc
     2941 cttgggcggc atgtactcgt cgtcggcctt gtcggtgcgc cgcttgctgg gctgcacccg
     3001 cggcggctcg cccggcatct tcgggtagtc gggcgggaag ttgagctcgc cgaggccccg
     3061 ctcgtcgacg tcgcgctgcc agagcgcgag tgccccggac acgtccccga cggcgccgtc
     3121 gatgtcggcc caggcatcac cgcggtcctc caggacgtcg ggcacggtca acacggtgaa
     3181 gtcgcccggg ctgaccgtct cgagctctgc ccacgtcacc ggcatcgaca ccggggctcc
     3241 gggcagcggc cgcgggctgt agggcgaggc gatggtgcgg tcacggcagg cctggttgaa
     3301 gtcgacgaag acccgttcgc cgcgttcctc cttccaccac gaggaggtca cgaggtcggg
     3361 aagccggcgc tcgagctcgc gcgcgatccc gatgacgccg tgccgcacgt cgaggaactc
     3421 gtgcgtcggg ccgatgcggg cgtagacgtg caccccccgg ttgccgctgg tcttggccca
     3481 gccggtgagc cccacctcgg tgagcagttc acgcagcgcg accgcagcct ccacggcatc
     3541 cgcgaacgtg cgcccaggct ggggatcgag gtcgatgcgc agctcgtcgg ggttgtcgtt
     3601 gttcgccgtg cgcaccggcc acgggtggta ggtgacggtg ttcatctgca ccgcccagac
     3661 ggcgctggcc acctcgtcga tcacgatctg cgcgtggcgt cgaccgctgg ggtagcggca
     3721 cgagacggtg cggacccact cgggaagacc cttgggaggg ttcttctggt agaactcctc
     3781 gccctcgatg ccgtccggaa agcgctggag ggtcaccggg cggtcaccga tgtggtggac
     3841 cagtgagtca ccgacggcca tgacgtaggc cgcgagatca cccttggtga tgccggcacc
     3901 aggccacatg agccggtccg ggctgctcag gcgcacctga cggggcccgt tcgggccctc
     3961 gatctccagc acctgggcat caaccatggc gcgagcctaa ccagcggcac cgacacacgg
     4021 gcccgggaag ccgagccacg gcatcacggt gggcctgcgc gccacgacat ccgaagggtg
     4081 cccttctcag cgggaaccat ccgaagtggg cgttcgacag tgggaaccat ccgaggtggc
     4141 tcgctgttgg tccagacatg acgatgaaac cgagtgaccg ttcctacgcc gtcgagcgca
     4201 ccgaggagga gtggaaggcc gccctgtccc ccgcggagta ccaggtgctg cgtcaggccg
     4261 gcaccgagcg gcccttcgtc ggcgagtaca ccgatgccaa gaccgtcggc acgtacgcct
     4321 gccgggcgtg cggtgccgag ctgttcacct ccgagaccaa gttcgacagc cactgcggct
     4381 ggccgtcgtt cttctcgccg ctcgcgggcg agtcggtcga gctgctcgag gaccggtcgc
     4441 tgcctggacg cccgcgcacc gaggtgcgct gtgcgacctg cggcagccac ctcgggcacg
     4501 tgttcgaggg cgagggctac gacacgccga ccgaccagcg ctactgcatc aactcgatca
     4561 gcctgaccct gcacccgaag gacgagcccg ccgctggctg acccgcctcg acttttcctc
     4621 cccatgaggc aagcccgagg ttcgcaaggg atcgcatccc ttgcgaacct cgggtttccc
     4681 atgtccagca cggagcggcc gcgttgtcca caggccggat gccgttcgct cgtcatccac
     4741 aggcccctca cctggcgtgc ccagcacctg ccggttcggc catcgtgacg ggatgacgac
     4801 gacggtggat cccgcgctgc gagctctggc cgcggggcag cacgggatgt tcacgctggc
     4861 ccaggcgcgc acgctgggtg tcagcccctt ggtcctggag cgtctggtcc gcgagaaccg
     4921 actgctccat cccggccgcg ggctgtatgc cgtcacggag gatgtcgacc aagcccctga
     4981 gccatggcac cggcacctct gcgtgggggc gcacctgctc tatcgcgacg cgtcactgac
     5041 cagcgccagc gctgcactgg cccacggcct gcccgtgtgg ggagtcgatc tgagcagacc
     5101 cgacctgcat cggcctgtcg accgcgccgt cggtgtcaag gcgtttcggg tgcggcctcg
     5161 accgaagaac cccctacgac ccgagccggt caccaccgag cttggccgcg ccgacgaggt
     5221 ggccacctcc gtcgtgcagt tggccctgga tcatggtccg gtggccggca ccgtcagcgc
     5281 ggacaacgcc gtccaccgag gtctggtgac gatcgacgcg ctgacgactg ccggtgacct
     5341 ggtggcgtcc tggcccgggt catcacgtgt ccggtccatg ctcgccttcg tcgacggccg
     5401 gtcggagtcc gtgggtgaga cccggtgccg catcgaactc gtgacgcacg gcatcccgct
     5461 ggtgccgcag gtgccgatcc gtgaccgcga cgggaacgtg ttcgcccggg tggacttcgt
     5521 catcgagggg tacaaggtcg tcgtcgagtt cgacgggagg gtgaagtacg ccgaaggaga
     5581 caaacaggtg ctgtgggatg agaagcgtcg cgaggatcgg ctgcgcgccc tgggctacct
     5641 cgtggtgcgg atcacctggg ccgatctcga gcaccccgga gcggccgttg cgaaggttcg
     5701 gcgcgcgatg gcccaggcgg cctgacctgc ccgagccttg acaagggaaa cccgaggttc
     5761 gccggggaag gcttgccttg cgaacctcgg gcttccccca tggggaggaa aagtcaggcg
     5821 cagggattcg gggttggggc gcgggacagg gctgggggtc agcgctggcg ggccaccagg
     5881 gtgcgcacgt ccacgcgcgg ccccgtgtag aacggcacct cctcgcgcac gtgcatccgg
     5941 gcccgggagg cccgcagctc tcgcatgagg tcgacgatgc ggtgcagctc gtcggcctcg
     6001 aacgcgagga tccactcgta gtcgttcagg gcgaacgagg cgatggtgtt ggcgcgcacg
     6061 tccgggtagt cgcgagccat ctggccgtgt tcacgcagca ggtcgcgccg ctcggcatcc
     6121 tcgagcaggt accaatcgta ggagcgcacg aacgggtaca cgcacacgta gtcgcgggcc
     6181 acctcgtcgg ccatgaacgc cgggatgtgg ctcttgttga actccgccgg ccggtgcagg
     6241 gcggccaccg accacaccgg gtcgaggtgc tggccgtatg ccgtgcggag cagcgcgtgg
     6301 taggcctcct ggagcgcctc gatcgtctcg gcgtgccacc acaccatgac gtcggcgtcg
     6361 gcgcgcagcc cggccacgtc ataggtgccg cgcacgacga cgtccttcgc ggcgaggtcg
     6421 gtgaacaact cctcgacctc tgccgcgagg gcgtcgcggt cctcgtcgcc gatcggctcg
     6481 gccacggcga acaccgacca catggcatac ctgatcgtgt cgttgatctc gcggatcctg
     6541 gcggcgttgg ggttgcctgg gacgggtcgg gctggctcgc tcatgcgcgg tgctctcctt
     6601 ggtgcggcgt gctcggaatg gtgctcaggt ggctcagggc ggctcgggcc gcgcgggtgg
     6661 cggaggcgat gacggccggg ataccgaccc cgtcgtaggc ggcaccggcc acctccaggc
     6721 cgggcagacg ggcgacgtcc gcgcgaaccg aggccacggc atccacgtgg ccgacggcat
     6781 actggggcag cccaccgccc caccgctgga cgtgcgcgtc gacgacccgg ggcagtgggc
     6841 gccccagggc ctcccccacc tcggcgacgg agacggcgac gaggtctgcg tcgtcgcgct
     6901 gcagcacggc ctcctcgcgc gccctgccga gcgaggcccg caggtgcacg acgtcgtcgg
     6961 agacctcacc ggtccacgcc cacttgcgaa agctgaacgt gctcgccttg atggcgcgcc
     7021 cttcgaccgg cgggacgagg aaccccgatc cccggacctc cgcgaggtca cggcgttcca
     7081 ccgcgagggt cacgacggca ctggatgccg tctc
//
LOCUS       DAOSOC010000131         6969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_10886_length_6969_cov_9.330344, whole genome shotgun sequence.
ACCESSION   DAOSOC010000131 DAOSOC010000000
VERSION     DAOSOC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10886_length_6969_cov_9.330344"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1268
                     /gene="pstS"
                     /locus_tag="PL014_09835"
     CDS             162..1268
                     /gene="pstS"
                     /locus_tag="PL014_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005271399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOT57434.1"
                     /translation="MKLHRIGTTATLAVVASIALAACGNDNPTGTTSPAASEGATALT
                     GTLKASGASSQESAMTAWIAGYQGVQPEVTVNYDSVGSGAGRENLLAGAVDFAGSDAA
                     LDEEERAKVVETCGPKGAMNIPVYISPVAVPFNLKGVTELNLKPDTLAKIFDRKITVW
                     NDPAIAADNPGAALPATKITVVNRSDESGTTENFMEYLSAAAPDSWSYEADKAWPVTG
                     GEAAAQTTGVIEVVGRTDGAIGYADASAVGTLSSAKIGVGDEFVAFSPEAAAKVVDAS
                     KPAESGIEGDLALELARDTTESGAYPIVLVSYHIVCSSYSDPAKAELVKDFVGYVVSE
                     AGQKASAEAAGSAPISADLRTKAEASLALITSGS"
     gene            1319..2251
                     /gene="pstC"
                     /locus_tag="PL014_09840"
     CDS             1319..2251
                     /gene="pstC"
                     /locus_tag="PL014_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOT57435.1"
                     /translation="MTTSPTTSSPKVRRRPGDVVFGGLSAASAFSILLILAGVALFLL
                     VEAAPVLSADSSDISGGEGFLSYVAPLVFGTLLASIIAMVVATPVAVGIALFVSHYVP
                     RRIGTSVGFVIDLLAAVPSVVFGMWGFQVLAPRLVPLYDWLEANVGFLPFFESASATG
                     RTILTASLVLAVMILPIITAVSREVFLQTPRLHEEAALALGATKWEMIRTTVLPFGKP
                     GVIGGTMLGLGRALGETMAVAIILSPGVFTLNLINSGNNTVPAEIALNFPEAAGLRLS
                     ELIAAGLVLFGITLGVNLAARYIVNRRAEFSGAN"
     gene            2251..3441
                     /gene="pstA"
                     /locus_tag="PL014_09845"
     CDS             2251..3441
                     /gene="pstA"
                     /locus_tag="PL014_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005271394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOT57436.1"
                     /translation="MSTPTMTPGAGAGAGAGADPGTTTETKPRQRKLSEIPGVELEVV
                     RPSSPRPALITLGLTAASAVALVVLGLNPALALAAAFIAYLIAVYAVYRRASGPRVAA
                     DQVVRGLVGGAFVIAVIPLLSLLWTVVSKGAATAFDPGFLTQTMNGVTGLQDQATQRD
                     GAPMVGGAYHAILGTLIITGIAAVISVPIGLFTAIYLVEYQPKGRLASMIRFLVDVMT
                     GIPSIVAGLFAFALWAVLFGVGTKNGAAGAVALAVLMIPTVVRNSEEMLRIVPNELRE
                     ASLALGVPQWRTIAKVVLPTAVSGLASGVTLAIARVIGETAPLLVAVGYARGLNVDPT
                     SGPMNTLAVYAYYMFTKPLSPGFREPSLDRAWAAALLLVIIVVLLNLVARLIARAFAP
                     KTSR"
     gene            3465..4244
                     /gene="pstB"
                     /locus_tag="PL014_09850"
     CDS             3465..4244
                     /gene="pstB"
                     /locus_tag="PL014_09850"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOT57437.1"
                     /translation="MSKRIDVKDLDIYYGSFHAVKNVSMTIEPRSVTAFIGPSGCGKS
                     TVLRTLNRMHEVIPGARVEGEVLMDDKNLYSKDTDPVDVRRQVGMVFQRPNPFPTMSI
                     RENVLAGVTLNSKRLSKAAADELTEQSLRGANLWTEVKDRLDRPGSGLSGGQQQRLCI
                     ARAIAVQPEVILMDEPCSALDPISTLAIEDLIEELKNDYTVVIVTHNMQQAARVSDRT
                     GFFNLEATGMPGELVEMDDTTKIFSNPSVKATEDYISGRFG"
     gene            complement(4278..5222)
                     /locus_tag="PL014_09855"
     CDS             complement(4278..5222)
                     /locus_tag="PL014_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT57438.1"
                     /translation="MRIPAAGTLPWRLRDGELEVALVHRPRYDDWSWAKGKLDPGEDW
                     AVAAVRETVEETGLVVRLGPPLPEARYTLLGRDGTPDEKVVRYWTAQVTGGDGHLLNE
                     IDEVVWLDVPAAHTRLDYARDRDQLLALVRLHQTARLDTWPLVLVRHAHAVARGEWSG
                     ADPLRPLSAEGERRSAALVPVLSAYGVSRVLTSPSERCLRTVEPFTDAAGVRLRTRTG
                     LSEEGFEADPRTAPAHLGRLLDRGEPAVLCTHGPVLPSLLDDLEGRLDLDQPASVEII
                     EEFAEARDDRLAKGEALVCHIVGTGDDARIVAVERHLP"
     gene            complement(5260..>6969)
                     /gene="ppk1"
                     /locus_tag="PL014_09860"
     CDS             complement(5260..>6969)
                     /gene="ppk1"
                     /locus_tag="PL014_09860"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOT57439.1"
                     /translation="FPVLTPLAVDPAHPFPYISGLSLNLAVVLVNPRTGKEHFARVKV
                     PPVLPRMLRVADSADESPLADMYATRFVPLEDVIAAHLDHLFPGMEIREHFTFRVTRN
                     EDLEVEEDDAENLLTALERELTRRRFGPPVRLEVEEDMDDHVLDLLVRELGVAGSEVY
                     RLPAPLDLRALNVIADLERSDLHFDPFVARTNPDLAPTESSKARDIFASIRKQDVLLQ
                     HPYDSFSTSVQAFIEQAAADPRVLAIKQTLYRTSGDSPIIDALIDAAEAGKQVLAVVE
                     IKARFDEENNISWARKLEHAGVHVVYGIVGLKTHAKLCLVVRQEAEGLRRYCHVGTGN
                     YNPKTARLYEDLGLLTDDPQVGEDLTRLFNQLSGVAPRSRFKRLLVAPRSVRSGLIDL
                     IDAEIANEEREPGSGLIAWKVNSIVDEQLIDALYGASRAGVRVQLWVRGICALRPGIP
                     GVSENIEVRSILGRFLEHSRIFLFGGGGETVALIGSADMMHRNLDRRVEALVHLVDPR
                     HLADLEALMKRGMSDEYMHWKLGADGRWIRHHLDAEGAPLTDLQTAMIEMHAKRRRKA
                     RRR"
BASE COUNT         1018 a         2534 c         2346 g         1071 t
ORIGIN      
        1 gggaaggggg tgggggtgtt cagcagcgcg tcaccgtcca ttcaccatcg gccaccggat
       61 cggacacctg cgctccttag cgtctggtcc agtcggcagt gtcgctggcc cgcgcggtcc
      121 tgacccgggc cgtgccccca tcatcaagag aggcaccatc cgtgaagctc caccgcatcg
      181 gcaccacggc caccctcgcc gtcgtggcgt ccatcgctct ggcggcctgt ggcaacgaca
      241 acccgaccgg cacgacctcg ccggccgcgt ccgagggcgc caccgccctc accggcaccc
      301 tcaaggcctc cggcgccagc tcgcaggagt cggccatgac cgcgtggatc gccggttacc
      361 agggcgtgca gcccgaggtc acggtcaact acgactccgt cggctccggc gccggacgtg
      421 agaacctgct cgccggtgcc gtcgacttcg ccggctcgga cgcagccctg gacgaggagg
      481 agcgcgccaa ggtcgtcgag acctgcggcc ccaagggcgc catgaacatc ccggtgtaca
      541 tctccccggt cgccgtcccc ttcaacctca agggcgtcac cgagctgaac ctcaagcccg
      601 acaccctcgc caagatcttc gaccgcaaga tcaccgtctg gaacgacccg gccatcgccg
      661 ccgacaaccc gggcgctgcc ctgccggcca cgaagatcac cgtggtcaac cgctccgacg
      721 agtcgggcac gaccgagaac ttcatggagt acctgagcgc cgccgctccc gactcgtgga
      781 gctacgaggc cgacaaggca tggcccgtca ccggtggtga ggccgccgcg cagaccaccg
      841 gcgtcatcga ggtcgtcggc cgcaccgacg gggccatcgg gtatgccgac gcctccgccg
      901 tgggcacgct ctcctcggcg aagatcgggg tcggcgacga gttcgtcgcc ttctcgcccg
      961 aggccgctgc caaggtcgtc gatgcctcca agcccgccga gagcggcatc gagggtgacc
     1021 tcgccctgga gctcgcccgc gacaccaccg agtccggcgc gtacccgatc gtcctcgtga
     1081 gctaccacat cgtgtgctcc tcctacagcg acccggccaa ggccgagctc gtcaaggact
     1141 tcgtcggcta cgtcgtctcc gaggcgggcc agaaggcctc cgccgaggca gctggctccg
     1201 ccccgatctc cgcggacctg cgcaccaagg ccgaggcctc gctggccctc atcacctccg
     1261 gtagctgacc cgcacccccg atcgatccga tcgacccgat agatccagaa ggacctccat
     1321 gaccacgtca cccacgacct cgtccccgaa ggtgcgtcgc cgcccgggtg acgtggtctt
     1381 cggagggctg tccgcggcat ccgcgttcag catcctgctc atcctcgcgg gggtcgcgct
     1441 cttcctcctc gtcgaggccg caccggtcct gagcgccgac agctccgaca tcagcggggg
     1501 agaggggttc ctcagctacg tcgccccgct ggtcttcggc acgctcctcg cgagcatcat
     1561 cgcgatggtc gtcgccaccc cggtggccgt cggcatcgcc ctgttcgtct cgcactacgt
     1621 gcctcggcgg atcggcacct cggtcgggtt cgtcatcgac ctgctcgccg ccgtgcccag
     1681 cgtcgtcttc ggcatgtggg gcttccaggt gctggctccc cggctcgtgc cgctctacga
     1741 ctggctcgag gcgaatgtcg gcttcctgcc gttcttcgag tcggcgtcgg ccaccggccg
     1801 caccatcctg accgcctccc tcgtgctcgc cgtgatgatc ctgccgatca tcacggcggt
     1861 ctcgcgcgag gtgttcctcc agaccccgcg gctgcacgag gaggccgccc tcgccctcgg
     1921 cgcgacgaag tgggagatga tccgcacgac ggtgctgccc ttcggcaagc ccggcgtcat
     1981 cggcggcacc atgctcggcc tgggccgcgc gctcggcgag acgatggccg tcgcgatcat
     2041 cctctcgccc ggggtcttca cgctgaacct gatcaacagc ggcaacaaca cggtgcccgc
     2101 agagatcgcg ttgaacttcc ccgaggcggc cgggcttcga ctgtccgagc tgatcgccgc
     2161 cggcctcgtg ctcttcggca tcaccctcgg ggtgaacctc gccgcccgct acatcgtcaa
     2221 ccgccgcgcc gagttctccg gagccaactg atgagcaccc ccaccatgac gcccggcgcc
     2281 ggagccggag ccggtgccgg cgctgacccc ggtaccacca ccgagaccaa gccgcgccag
     2341 cgcaagctct ccgagatccc cggggtcgag ctcgaggtcg tgcgcccctc gtccccacga
     2401 cccgcgctga tcacgctggg cctcaccgcg gcatccgccg tggccctcgt cgtgctgggc
     2461 ctgaacccgg cgctcgccct cgctgccgcc ttcatcgcct acctcatcgc cgtgtacgcg
     2521 gtctaccgcc gagcaagtgg cccgcgggtc gccgccgacc aggtcgtgcg cgggctcgtg
     2581 ggcggggcgt tcgtcatcgc cgtcatcccg ctgctctccc tgctgtggac cgtggtgtcc
     2641 aagggcgcgg caaccgcgtt cgacccgggc ttcctcacgc agaccatgaa cggcgtcacc
     2701 ggcctgcagg accaggcgac ccagcgtgac ggcgccccca tggtcggcgg cgcgtaccac
     2761 gccatcctcg gcaccctgat catcacgggt atcgccgcgg tcatctcggt gccgatcggt
     2821 ctgttcacgg cgatctacct cgtcgagtac cagcccaagg gccggctcgc ctcgatgatc
     2881 cgcttcctcg tcgacgtcat gaccggcatc ccgtccatcg tcgcgggtct gttcgccttc
     2941 gccctgtggg ccgtgctctt cggggtcggc acgaagaacg gggcggccgg tgccgtcgcg
     3001 ctcgccgtgc tcatgatccc gacggtggtg cgcaacagcg aggagatgct gcgcatcgtc
     3061 ccgaacgagc tgcgcgaggc ctcgttggcg ctcggtgtgc cgcagtggcg cacgatcgcc
     3121 aaggtcgtcc tcccgactgc ggtgtccggc ctggcctcgg gcgtgacgct ggccatcgcg
     3181 cgggtgatcg gcgagaccgc gccgctgctc gtcgccgtcg gttacgcccg cggcctgaac
     3241 gtcgacccca cgtcgggccc gatgaacacc ctggccgtct acgcgtacta catgttcacc
     3301 aagccgctca gcccgggctt ccgcgagccc agcctggacc gcgcctgggc cgccgcgctg
     3361 ctcctcgtga tcatcgtcgt cctgctcaac ctcgtggcac ggctcatcgc ccgagccttc
     3421 gcccccaaga ccagtcgctg aaccccaaag gactccagac ccccatgtct aagcgcatcg
     3481 acgtcaagga cctcgacatc tactacggct ccttccacgc cgtgaagaac gtgtcgatga
     3541 ccatcgaacc ccgctcggtc acggccttca tcggcccgtc cggctgtggc aagtccaccg
     3601 tgctgcgcac cctgaaccgc atgcacgagg tcatccccgg cgcacgcgtc gagggcgagg
     3661 tcctcatgga cgacaagaat ctctactcca aggacaccga cccggtcgac gtgcgccgcc
     3721 aggtcggcat ggtcttccag cggccgaacc cgttcccgac gatgtccatc cgcgagaacg
     3781 tgctcgccgg ggtgacgctg aactccaagc gcctgtccaa ggcggccgcg gacgagctga
     3841 ccgagcagtc gctgcggggc gcgaacctgt ggaccgaggt caaggaccgg ctcgaccgcc
     3901 ccggctccgg cctgtccggc gggcagcagc agcgcctgtg catcgcccgt gcgatcgcgg
     3961 tgcagcccga ggtgatcctc atggacgagc cgtgttccgc tcttgacccc atctcgacgc
     4021 tggccatcga ggacctcatc gaagagctga agaacgacta caccgtggtc atcgtcacgc
     4081 acaacatgca gcaggccgcg cgggtctccg atcgcaccgg cttcttcaac ctcgaggcca
     4141 ccggcatgcc cggcgagctc gtcgagatgg acgacacgac caagatcttc agcaacccgt
     4201 cggtcaaggc caccgaggac tacatctccg gccgcttcgg ctgatcggat gccgtgcggc
     4261 tggctcggat tcgcgcccta cggcaggtgc cgctcgaccg ccacgatccg ggcgtcatcg
     4321 ccggtgccga cgatgtggca gaccagcgcc tcacccttgg cgaggcggtc gtctcgggcc
     4381 tcggcgaact cctcgatgat ctcgaccgag gcgggctggt cgagatccaa ccggccttcg
     4441 aggtcatcga gcaggctggg gagcacgggg ccgtgggtgc agagcaccgc tggttcgccc
     4501 cggtcgagca gccggccgag gtgggcggga gcggtgcgag ggtcggcctc gaagccctcc
     4561 tccgacaacc cggtccgggt gcgcagccgc accccggctg cgtcggtgaa cggctccacg
     4621 gtgcgaagac accgttccga cggtgaggtg agcacccggc tcacgccgta ggccgagagc
     4681 accggcacga gggcagccga ccggcgctcg ccctccgccg acaaggggcg cagggggtcg
     4741 gcacccgacc actcaccccg ggcgaccgcg tgcgcgtgcc gcacgaggac gaggggccag
     4801 gtgtcgaggc gggccgtctg gtgcaggcgc accagggcga gcagctggtc gcggtcacgg
     4861 gcgtagtcca accgcgtgtg ggcggccggc acgtcgagcc agaccacctc gtcgatctcg
     4921 ttgaggaggt ggccgtcgcc gccggtgacc tgggcggtcc agtagcgcac gaccttctcg
     4981 tcaggggtgc cgtcgcggcc gagaagggtg tagcgggcct cgggcagcgg cggccccagt
     5041 cgcacgacga gcccggtctc ctcgacggtc tcgcgcacgg ccgcgaccgc ccagtcctcg
     5101 cccgggtcga gcttgccctt ggcccaggac cagtcgtcgt aacgagggcg gtgcacgagg
     5161 gcgacctcga gctcaccgtc gcgcagccgc cacggcaggg tacccgccgc ggggatcctc
     5221 atcgggccgc ctccgtgcgg ccagcccccg ggtagccggt caccgtcggc gggccttgcg
     5281 gcgccgcttg gcgtgcatct cgatcatcgc ggtctgcagg tcggtgagcg gggcgccctc
     5341 ggcatcgagg tggtggcgga tccagcggcc gtcagccccg agcttccagt gcatgtactc
     5401 gtcgctcatg ccgcgcttca tcagcgcctc gaggtcggcg aggtggcgcg ggtcgaccag
     5461 gtgcaccagc gcctcgacgc gtcggtcgag gttgcggtgc atcatgtcgg cgctgccgat
     5521 gagcgccacc gtctcgcctc cgccgccgaa caggaagatg cgggagtgct cgaggaagcg
     5581 gcccaggatc gagcgcacct cgatgttctc ggacacgccg gggatgccgg ggcgcagggc
     5641 gcagatgccg cgaacccaca gctggacgcg cactccggcg cgcgaggccc cgtagagcgc
     5701 gtcgatcagc tgctcgtcga cgatcgagtt gaccttccag gcgatcagac cgctgccggg
     5761 ctcccgctcc tcgttcgcga tctcggcgtc gatgaggtcg atgagccccg agcgcaccga
     5821 ccggggtgcc acgagcaggc gcttgaaccg gctgcggggg gcaacccccg agagctggtt
     5881 gaacaggcgg gtgaggtcct cgccgacctg cgggtcgtcg gtgagcagcc cgaggtcctc
     5941 gtagaggcga gccgtcttgg ggttgtagtt gccggttccg acgtggcagt agcggcgtag
     6001 cccctctgcc tcctggcgta ccacgaggca gagcttggcg tgggtcttca gaccgacgat
     6061 gccgtagacg acgtgcacac cggcgtgctc gagcttgcgc gcccaggaga tgttgttctc
     6121 ctcgtcgaag cgcgccttga tctcgacgac ggccagcacc tgcttgccgg cctcggcggc
     6181 gtcgatgagg gcgtcgatga tcgggctgtc accactggtg cggtacaggg tctgcttgat
     6241 ggcgagcacc cgcgggtcgg ccgcggcctg ctcgatgaag gcctgcaccg acgtcgagaa
     6301 cgagtcgtac gggtgctgga gcagcacgtc ctgcttgcgg atcgaggcga agatgtcgcg
     6361 ggccttcgag ctctcggtcg gtgcgaggtc ggggttggtg cgcgccacga acgggtcgaa
     6421 gtgcaggtcg gagcgctcga ggtcggcgat gacgttgagc gcgcgcaggt ccagcggcgc
     6481 cggcaggcgg tacacctcgg acccggcgac gccgagttcg cgcacgagca ggtccaggac
     6541 gtggtcgtcc atgtcctcct cgacctcgag gcgcaccggc gggccgaagc ggcgccgggt
     6601 cagctcacgc tcgagagccg tcagcaggtt ctcggcgtcg tcctcctcga cctcgaggtc
     6661 ctcgttgcgg gtgacccgga aggtgaagtg ctcacggatc tccatgccgg ggaacaggtg
     6721 gtccaggtgg gcggcgatga cgtcctcgag cgggacgaac ctggtggcgt acatgtcggc
     6781 cagcggcgac tcgtccgcgc tgtcggcgac gcgcagcata cgggggagca cgggcggcac
     6841 cttgacccgg gcgaagtgct ccttgccggt gcgcgggttg accaggacga ccgcgaggtt
     6901 cagggagagc cccgagatgt acgggaacgg gtgcgcgggg tcgacggcga gcggggtgag
     6961 caccgggaa
//
LOCUS       DAOSOC010000132         6825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_11199_length_6825_cov_10.259380, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000132 DAOSOC010000000
VERSION     DAOSOC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11199_length_6825_cov_10.259380"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PL014_09865"
     CDS             complement(<1..451)
                     /locus_tag="PL014_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOT57440.1"
                     /translation="MTAKLHPRVQPHFEMVLSRNPGETEFHQAVFEVMASLTPMIGTR
                     PEYAQWSVLSRMCEPERQIIFRVPWLDDQGRVQINRGFRVEFNSALGPYKGGLRFHPS
                     VNLSIIKFLGFEQVYKNALTGMPIGGGKGGSDFDPKGRSDTEIMRFCQ"
     gene            613..1086
                     /locus_tag="PL014_09870"
     CDS             613..1086
                     /locus_tag="PL014_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HOT57441.1"
                     /translation="MITVDELLASCDIALDAYAESCRALGDDLLNVRLDDVPGSNTAS
                     GLVAHVVGVMARWGRTVNRGIVVPRDRDAEFRATLTVDQAITMLEAGRASLHEDARAA
                     APHEAPRNPPAGDGLTRHRTQGAVLLHVFEELTQHLGHLDVTRDVLLARRRPAAG"
     gene            1092..3260
                     /gene="ligA"
                     /locus_tag="PL014_09875"
     CDS             1092..3260
                     /gene="ligA"
                     /locus_tag="PL014_09875"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT57442.1"
                     /translation="MNATPIDPASTPPTDADAVPDEARHAWVTLAEQVRAHQFAYHVK
                     DAPTISDGEYDALIRSLTALEDEHPALRTPDSPTQQVGGAIFSTDFQAVDHLERMLSL
                     DNAFDADELRTWAARVERDAGGAAHHFLCELKIDGLAINLLYEGGRLTRALTRGDGRT
                     GEDVTLNVRTIAGIPETLSGDDVPELVEIRGEVYFPLAAFADLNASLVAAGKAPFANP
                     RNTAAGSLRQKDPRVTATRDLRMLVHGIGAHRGLELTRQSEAYDRMREWGLPVSDQFR
                     VVETVDEVLAFITHTGEHRHDNAHDIDGVVVKVDEVAVQRQLGSTSRAPRWAIAYKYP
                     PEEVNTKLLDIQVNVGRTGRVTPYGVMKAVVVAGSTVTQATLHNADEVRRKGVLIGDT
                     IVLRKAGDVIPEILGPVVDLRDGTEREFVMPTHCPSCGTALAPEKEGDKDIRCPNARS
                     CPSQLRERLFALASRGAFDIEALGWEGSIALLESGVLEDPELGGPTEARLFSLTVDDV
                     ARVPLYTRTAKKTDPPQDVVDGRVLSANGHKLVANLDSAKAQPLWRVLVALSIRHVGP
                     TAARALASHLGSMDAVRGASLEELAGVEGVGPVIAESVRDWFDKPGNEWHVAIVDRWA
                     ADGVRMVDERDESVEQTLAGLTIVVTGSLEGFSRDETKEAILSRGGKASGSVSKKTDY
                     VVVGENAGSKEDKARELGLTILDEAGFRRLLDTGNPDPVS"
     gene            complement(3390..5198)
                     /locus_tag="PL014_09880"
     CDS             complement(3390..5198)
                     /locus_tag="PL014_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57443.1"
                     /translation="MPDNDRTSARLPAAVLAHLTEVLGATPQTTGKTEAELPGGERLV
                     AYHAVSAEKPNAVVAVVAAADGTITPRADFTAALGHDPFIPHFDPPVLGTVPPRRRPV
                     TINPRRNDWRLDQCDRRSEKITVTIPPTGTAPKADVYLLADTTGSMAPVIDAVKAGAD
                     AILSDPALAAFDVAWGVGNYRDFTVPAPNSYAFQHQLAPTTSTAAASGAISTWNADEG
                     GDTPEGQLYALQELATNTSIGWRTDSKRIIVWFGDAPGHDPICTDLTGLPAAITEATA
                     TAALTGANITVVAVSTTTGVADALDGDPNSGSFDYPTCAANGTAGQATRITAATGGEH
                     RTGVDPGSIVSTLSDLIADAVQSTGNVHLVATGDTAQFVESITPAAGYGPLAGDETHT
                     LTFEVVWVGTKACAERDQELTGTIDVVADDVVVASKPVRVVVPKCRYHYVVEVVCGER
                     PPLRGRDDHDDRGDHPEGPGGGHPTKDDGPCAVVPGRYATAVLISNPSTCTAVIEKRF
                     APLVVDGKTIGREPDIQPAKPFAKLELPANHATMDDCCALHEAVDHGPGLLLGVLDIV
                     SDRPLEVRAVHTAHGAQTQTGAPSITTRAVDPRRAP"
     gene            complement(5300..5965)
                     /locus_tag="PL014_09885"
     CDS             complement(5300..5965)
                     /locus_tag="PL014_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HOT57444.1"
                     /translation="MLTDTASTQLVLDLVGVFVFALTGAIVGVRRGLDVFGVLVLGWI
                     AGLGGGIIRDVFLGITPPVAVSDWRLLAAAVVAGLAVFLLHGTWQEVAQRHPDARLGR
                     VSFTVRLLDAAGLAVFAVSGALVALDAQAGALAATLIGGITGIGGGLLRDVLTGQVPE
                     VLRRELYAVPALAGAGLVVLLHEAGALTTLTAWAAVGFVFVTRVVAVVLNLNAPRPLR
                     SRA"
     gene            6011..6307
                     /gene="gatC"
                     /locus_tag="PL014_09890"
     CDS             6011..6307
                     /gene="gatC"
                     /locus_tag="PL014_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOT57445.1"
                     /translation="MADLTRDDVAHLAGLARIDLSDAELDRMVGELGVILEAVATVQQ
                     APIADVEPMSHPMPIVNVTRPDVVRPSLSPQDALSGAPESEQQRFAVPRILDVE"
     gene            6419..>6825
                     /locus_tag="PL014_09895"
     CDS             6419..>6825
                     /locus_tag="PL014_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOT57446.1"
                     /translation="MTTDLTRSTAADLADLLAQGSVSAREVTQAHLDRTAAVDGAVHS
                     YLHVDADGALAQADEVDRRRAAGESLHAMAGVPIAVKDVMTTRGLPTTCGSKILQGWV
                     PPYDATLVTRLREAGMPILGKTNMDEFAMGSST"
BASE COUNT          988 a         2482 c         2427 g          928 t
ORIGIN      
        1 actggcagaa gcgcatgatc tcggtgtcgg agcggccctt ggggtcgaag tcggacccgc
       61 ccttgccgcc gccgatcggc atgccggtca gggcgttctt gtagacctgc tcgaaaccga
      121 ggaacttgat gatcgacagg ttgacgctcg ggtggaagcg cagaccgccc ttgtaggggc
      181 ccagcgccga gttgaactcc acccggaagc cgcggttgat ctgcacccgg ccctggtcgt
      241 cgagccacgg cacgcggaag atgatttgcc gctcgggttc gcacatgcgc gagagcaccg
      301 accactgcgc gtactcgggg cgggtgccga tcatgggggt caggctcgcc atgacctcga
      361 agacggcctg atggaactcc gtctcaccgg gattgcgcga gaggaccatc tcgaagtgcg
      421 gctggacccg ggggtggagt ttcgcggtca tgggcccact ctggcaaacc tcgaccgctg
      481 gtgtcgtgcc gggtcaccca gcggaaggcc ggcgggcgtc gcccgcgggg cgcgcgagcc
      541 ggtggcgcac tccgcgcccg cagccgacct cgaccgtcgg tgcccgtcgg tgcccgtcgc
      601 tacggtggcc gggtgatcac cgtcgacgaa ctgctcgcct cctgcgacat cgccctggac
      661 gcctacgccg agagctgccg cgccctgggt gacgacctgc tcaacgtccg gctcgacgac
      721 gtgccgggga gcaacacggc atccggtctc gtcgcgcacg tggtcggggt catggcccgg
      781 tggggccgca cggtcaaccg cggcatcgtc gtgccccgcg accgcgatgc cgagttccga
      841 gcgaccctca ccgtcgacca ggcgatcacc atgctcgagg ccggccgagc cagcctgcac
      901 gaagacgccc gggcggcggc accgcacgag gcgccgcgca acccacccgc gggcgacggc
      961 ctcacccgcc accgcacgca gggtgccgtg ctgctccacg tgttcgagga gctcacccag
     1021 cacctggggc acctcgacgt cacgcgcgac gtgctgctcg cccgtcggcg ccctgcggca
     1081 ggatgaccgc cgtgaacgcc acgccgatcg accccgcctc gacgccaccc accgacgccg
     1141 acgccgtgcc ggacgaggcc cgtcacgcct gggtcaccct cgccgagcag gtccgggccc
     1201 accagttcgc ctaccacgtc aaggacgcgc cgacgatcag cgacggcgag tacgacgccc
     1261 tcatccgctc gctgacggcg ctcgaggacg agcaccccgc cctgcgcacc ccggacagcc
     1321 cgacccagca ggtgggcggg gcgatcttct ccaccgactt ccaggcggtc gaccacctcg
     1381 agcggatgct cagcctcgac aacgccttcg acgccgacga gctgcgcacc tgggcggcgc
     1441 gagtggagcg tgatgcgggt ggcgcggcgc accacttcct gtgcgagctg aagatcgacg
     1501 ggctcgcgat caacctgctc tacgagggcg gccggctcac ccgggcactc acccgcggcg
     1561 acgggcgaac cggtgaggac gtcaccctca acgtgcgcac catcgccggc atccccgaga
     1621 cgctgagcgg tgacgacgtc cccgagctcg tcgagatccg cggtgaggtc tacttcccgc
     1681 tcgccgcctt cgccgacctc aacgcgagcc tcgtcgccgc gggcaaggcg ccgttcgcca
     1741 acccgcgcaa caccgctgcg gggtcgctgc gccagaagga cccgcgggtc accgcgaccc
     1801 gcgacctgcg catgctcgtg cacggcatcg gcgcgcaccg ggggctggaa ctgacccggc
     1861 agagcgaggc ctacgaccgg atgcgggagt ggggtctgcc ggtgtccgac cagttccgcg
     1921 tggtcgagac cgtcgacgag gtgctcgcct tcatcacgca caccggcgag caccggcacg
     1981 acaacgccca cgacatcgac ggcgtcgtcg tcaaggtcga cgaggtcgcg gtccagcgcc
     2041 aactcggctc gacgtcgagg gcaccccggt gggccatcgc ctacaagtac ccaccggagg
     2101 aggtcaacac caagctcctc gacatccagg tcaacgtcgg acgcaccggg cgggtcacgc
     2161 cctacggggt catgaaggcg gtcgtcgtcg ccgggtcgac ggtcacccag gcgaccctgc
     2221 acaacgccga cgaggtgcgg cgcaagggcg tgctcatcgg cgacaccatc gtgctgcgca
     2281 aggcgggcga cgtcatcccc gagatcctcg ggccggtcgt cgacctgcgc gacggcaccg
     2341 agcgtgagtt cgtcatgccg acccactgcc cctcgtgcgg caccgcactg gcccccgaga
     2401 aggagggcga caaggacatc cgctgcccca acgcccgctc ctgcccgagc cagctgcggg
     2461 agcggctctt cgcgctcgcc tcacgcggtg cgttcgacat cgaggccctg ggctgggaag
     2521 ggtcgatcgc cctgctggag tcgggcgtcc tcgaggaccc cgagctcggc ggccccaccg
     2581 aggcccggct gttctcgctg accgtggacg acgtcgcccg ggtgccgctc tacacccgca
     2641 ccgcgaagaa gaccgacccg ccgcaggacg tcgtcgacgg ccgcgtgctc tcggcgaacg
     2701 gccacaagct cgtcgccaac ctcgactcgg cgaaggccca gccgctgtgg cgggtgctcg
     2761 tcgcgctgtc catccgccac gtgggcccga ccgccgcccg ggcgctcgcc tcccacctcg
     2821 gttcgatgga tgccgtccgc ggcgcctcgc tcgaggagct cgccggggtg gagggcgtcg
     2881 gcccggtcat cgccgagtcg gtgcgcgact ggttcgacaa gcccggcaac gagtggcacg
     2941 tcgcgatcgt cgaccgctgg gccgccgacg gtgtgcgcat ggtggacgaa cgggacgagt
     3001 cggtggagca gacgctggcc gggctcacga tcgtcgtcac cgggtcactc gaggggttct
     3061 cacgtgatga gaccaaggag gccatcctca gccgcggggg caaggcctcg gggtcggtgt
     3121 cgaagaagac cgactacgtc gtcgtcggcg agaacgccgg ctccaaggag gacaaggccc
     3181 gcgagctcgg gctgaccatc ctcgacgagg ccggcttccg ccgcctgctc gacaccggca
     3241 accccgatcc cgtgtcctga ccttccggcg ggaacagtca gcaaaacacg acccggctgg
     3301 cctggatcgg cgtggccagc cgggtcgagt ctgtgggcgg atcccccgag gcgtcgtgca
     3361 ggcggtcccg ggttaccagc agtgcgcact cagggagcgc gtcgcgggtc gacggcccgc
     3421 gtggtgatcg acggggcgcc ggtctgcgtc tgggcaccgt gtgcggtgtg cacggcccgc
     3481 acctccagcg gacggtcgga cacgatgtcg agcaccccga gcagcagacc cgggccgtgg
     3541 tcgacggcct cgtgcagagc gcagcagtcg tccatcgtcg cgtggttcgc gggcagttcc
     3601 agcttggcga acggcttggc cggctggatg tcgggctcgc gcccgatggt cttgccgtcg
     3661 accacgagcg gggcgaaccg cttctcgatg acggcggtgc acgtggacgg gttgctgatg
     3721 agcacggccg tcgcgtaccg gccgggcacg accgcgcagg ggccgtcgtc cttcgtgggg
     3781 tggccgccgc cggggccctc ggggtggtcg cctcggtcgt cgtggtcgtc gcgcccgcga
     3841 aggggcggcc gctcaccgca gaccacctcg acgacgtagt ggtagcggca cttcggcacc
     3901 acgacccgca ccggcttgct cgcgaccacg acgtcgtcgg cgacgacatc gatcgtgccg
     3961 gtgagctcct ggtcgcgctc ggcgcacgcc ttggtgccga cccacaccac ctcgaaggtc
     4021 agcgtgtgcg tctcgtcgcc ggcgagcgga ccgtaaccgg ccgcaggggt gatcgactcg
     4081 acgaactggg ccgtgtcgcc agtcgccacg aggtgcacgt tgccggttga ctgcaccgca
     4141 tcggcgatga ggtcgctgag cgtcgagacg atgctgcccg ggtcgacgcc ggtgcggtgc
     4201 tccccgccgg tggcggccgt gatccgggtg gcctgccccg cggttccgtt ggcggcacag
     4261 gtcgggtagt cgaaggatcc ggagttcggg tcgccgtcga gggcgtcggc gaccccggtg
     4321 gtggtgctca ccgccacgac ggtgatgttc gccccggtga gggcagcggt cgccgtggcc
     4381 tccgtgatcg ccgcaggcag gccggtgagg tcggtgcaga tggggtcgtg gccgggagcg
     4441 tcgccgaacc acacgatgat ccgcttgctg tcggtgcgcc acccgatgct cgtgttcgtc
     4501 gcgagctcct gaagggcgta gagctggccc tcgggcgtgt cgccgccctc gtcggcgttc
     4561 cacgtcgaga tggcgccgga ggcggccgcc gtgctggtcg tcggggcgag ctggtgctgg
     4621 aaggcgtagg agttgggggc tggcaccgtg aagtcgcggt agttgccgac tccccacgcg
     4681 acgtcgaagg ctgccagggc ggggtcgctg aggatggcgt cggcaccggc cttcacggcg
     4741 tcgatgaccg gcgccatgct gcccgtggtg tctgccagta ggtagacgtc ggccttgggc
     4801 gcagtgcctg tgggtgggat ggtgacggtg atcttctcgc tgcgccggtc gcactggtcg
     4861 agccgccagt cgttgcgtcg cgggttgatc gtcacgggcc gacggcgcgg cgggacggtg
     4921 ccgagcacgg gcgggtcgaa gtgcgggatg aacgggtcgt ggcccagggc tgccgtgaag
     4981 tcggcgcgcg gcgtgatggt gccgtcggct gcggcgacca cggccacgac ggcattgggc
     5041 ttctcggccg acacggcgtg gtacgcgacg aggcgttccc cgccgggcag ctcggcctcg
     5101 gtcttgccgg tggtctgcgg cgtggcaccg agcacctcgg tgaggtgtgc gagcaccgcg
     5161 gccggcaggc gcgcagaggt gcggtcgttg tctggcatgg cgttctcctt ggagcgtcgc
     5221 gggccgatcc ccagtccatc tggccgctca ccgtgaagga gccggactcc ccgtccctga
     5281 tacccgctcg tccgggtggt caggctcggc tgcgcaaggg gcgcggagcg ttcaggttga
     5341 gcacgaccgc cacgacccgg gtgacgaaga cgaacccgac cgccgcccag gcggtgagcg
     5401 tcgtgagggc ccccgcctcg tggagcagga cgaccagacc cgccccggca agggccggca
     5461 cggcatacag ctctcgccgc agcacctcgg gcacctgccc ggtgagcacg tcccgcagca
     5521 ggccgccgcc gatcccggtg atgcccccga tgagcgtggc tgccagcgcg cccgcctggg
     5581 cgtccagcgc cacgagggcg ccgctgacgg cgaacaccgc cagccccgcg gcatccagca
     5641 gccgcacggt gaacgagacc ctgcccagcc gcgcgtccgg gtgtcgctgc gcgacctcct
     5701 gccacgtccc gtggaggagg aagacggcca gccccgcgac caccgcggcc gcgagcaggc
     5761 gccagtcgct gaccgccacc ggcggggtga tgcccaggaa gacgtcgcgg atgatccctc
     5821 cacccaaccc ggcgatccag cccagcacga gcaccccgaa gacgtccagg ccccggcgca
     5881 cgccgacgat ggcgccggtg agcgcgaaga cgaacacgcc gacgaggtcg agcacgagct
     5941 gtgtcgaggc ggtgtccgtg agcaccggcc cagtctcgca gcccggcagc gggcccatag
     6001 actggcccgc atggccgacc tcacccgtga cgacgtggcg cacctcgccg gactcgcccg
     6061 catcgacctc tccgacgccg agctcgaccg catggtcggc gagctcgggg tcatcctcga
     6121 ggcggtggcg accgtgcagc aggcaccgat cgccgacgtc gagccgatga gccaccccat
     6181 gccgatcgtc aacgtgaccc gccccgacgt ggtgcgcccc tcgctcagcc cgcaggacgc
     6241 gctgtccggg gcgcccgagt ccgagcagca gcgcttcgcc gtgccgcgca tcctcgacgt
     6301 cgagtgacgc gcacgcccac cccgtgcacc acctgaccgc cacgcttcac cggatgccgg
     6361 ccacacggca tccgctcgcc gttcaccacc gcgccccgca ccccagagga accacgacgt
     6421 gaccaccgac ctgacccggt ccactgctgc cgacctcgcc gacctcctcg cgcaggggag
     6481 cgtgtcggcg cgcgaggtga cccaggcgca cctggaccgc accgcggccg tcgacggcgc
     6541 cgtgcactcc tacctgcacg tcgacgcgga cggtgccctc gcccaggccg acgaggtcga
     6601 ccgccgtcgc gccgcggggg agtcgctgca cgcgatggcc ggtgtgccga tcgccgtcaa
     6661 ggacgtcatg acgacccgcg gcctgccgac gacgtgtggc tccaagatcc tccagggctg
     6721 ggtgccgccg tacgacgcga ccctcgtgac gcgcctgcgc gaggccggca tgcccatcct
     6781 cggcaagacg aacatggacg agttcgccat gggctcctcg accga
//
LOCUS       DAOSOC010000133         6731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_11412_length_6731_cov_7.907430, whole genome shotgun sequence.
ACCESSION   DAOSOC010000133 DAOSOC010000000
VERSION     DAOSOC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11412_length_6731_cov_7.907430"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PL014_09900"
     CDS             complement(<1..329)
                     /locus_tag="PL014_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT57447.1"
                     /translation="MKRIAVTGSSGLIGTAVVAALTQRGDEVVRFVRRPARGRDEVQW
                     DPTSRTLDPRALDGVDGVVHLAGAGVGDKRWSPAYKREILSSRTDTTHALATAIAQVQ
                     HPVRFVSG"
     gene            complement(373..2226)
                     /gene="sucB"
                     /locus_tag="PL014_09905"
     CDS             complement(373..2226)
                     /gene="sucB"
                     /locus_tag="PL014_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HOT57448.1"
                     /translation="MSERVKMPALGESVTEGTVTRWLKGVGDTVEIDEPLLEVSTDKV
                     DTEIPSPIAGTLQEILVQEDDTVPVGADLAVIGDGDAPAASEPEAAPAEAAPEPAPVE
                     AEAAPAEAAPAPAPAESAPAEQAAPQGSADTSSDGGDGTTVTMPALGESVTEGTVTRW
                     LKQEGDDVEVDEPLLEVSTDKVDTEIPSPVAGKLNKILVQEDDTVPVGADLAVIGGSG
                     GGSAPAAAAEAPAPEPAAAAPAPAAPAPASAAPAPASAAPAPAAPAPASAAPAPAAPA
                     PAPAAPAPAAEAPAPAAAAPASSDDTEGRDAAAYVTPLVRKLAADHGIDLATLTGTGI
                     GGRIRKQDVMAAAEAKKAPAPAPEAPAPAAAAPAAPAKAPAATDSRRGTTEKMSRLRQ
                     TIARRMVESLQISAQLTTVVEVDVTRIAKLRDRAKADFAAREGTKLSFLPFFALAAVE
                     ALKAHPTVNASVDGDQVTYYGAEHLGVAVDTEKGLLVPVLKNAGDLNIAGLARGISDV
                     AERTRNNKIMPDDLSGGTFTITNTGSRGALFDTPIINQPQVAILGTGSVVRRPVVAMT
                     EDGGETIAIRSMVYLALSYDHRIVDGADAARFLTTMKQRLEEGAFEADLGL"
     gene            complement(2266..3648)
                     /gene="lpdA"
                     /locus_tag="PL014_09910"
     CDS             complement(2266..3648)
                     /gene="lpdA"
                     /locus_tag="PL014_09910"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOT57449.1"
                     /translation="MPATAEPTHDVVVLGGGSGGYACALRAAELGLSVALVEKDLLGG
                     TCLHRGCIPTKALLHAAEVADSAREATRFGVRATLDGVDLPGVTAYREGVVSRLHKGL
                     QGLVSSRKVDYVQGEGRLEGERTVVVGDRRLVGRHVVLATGSYARTLPGLELGGPIMT
                     SDGALALTEVPKSIVVLGGGVIGVEFASVMRSFGADVTIVEALPRLVAAEDPAVSTAL
                     ERAFKKRGIAFRTGVRFESATPTESGVTVRLEGGDELSADVLLVAVGRGPSTAGLGFE
                     DAGVSVERGFVITDERCRTSVDGVYAVGDIVPGLQLAHRGFAQGIFVAEDIAGLDPVP
                     LVESSIPRVTYCEPEIASVGLTQAQATEQYGDRVETYEYNLGGNGKSQILRTQGFVKL
                     VRVKDGPVVGIHMVGSRMGEQIGEAQLIVSWEAHAEDVAALLHAHPTQNEALGEAHLA
                     LAGKPLHAHN"
     gene            3721..4044
                     /locus_tag="PL014_09915"
     CDS             3721..4044
                     /locus_tag="PL014_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57450.1"
                     /translation="MAWWRRSRDKQAPHGGGDLSSARAHLQEFVGTRRGVEAYIEPAT
                     NITATTVVLIAHDGEWTRRALPSRPVAFEVARSLDIPVYDVNLTGYPARMRQWTARQR
                     RPDQR"
     gene            4162..4356
                     /locus_tag="PL014_09920"
     CDS             4162..4356
                     /locus_tag="PL014_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HOT57451.1"
                     /translation="MSGSKKPRATVDEDVKAKFRAALDAKKSHGGADVSDHSDHSKVD
                     HAHGPEASATQQMFRRKSGG"
     assembly_gap    4357..4456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4458..6017)
                     /locus_tag="PL014_09925"
     CDS             complement(4458..6017)
                     /locus_tag="PL014_09925"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOT57452.1"
                     /translation="MTSVSLTTRTPAELPVDCLVIGSVRTPDGTDLATGHALPRKAVV
                     HLQAVLADLEATGKADEVVRVVAVPGVKATSVVVTGLGEGTSRRATYPHTVLRSAAGA
                     ALRTVRGKRTVAVALPTPDVESLSAVADGAYAGCYVYDKSAPLAVVGAARRTVPARPG
                     KPTATTGATASGPRVLVVSGAGQGRAAKDAVTRAGILGSARDWARDLVNTPPNYLYPA
                     SFADAVKKRVAGSTARVTVSVLDDKALVKGGFGGIVGVGQGSVHPPRIVTMSYSPASP
                     KASVALVGKGITFDSGGLNIKPSSGMVTMKCDMAGAAAVAATVIAAAELALPVAVTGY
                     LCLAENMPSGTAQRPSDVVVMRNGTSVEILDTDAEGRMVLGDGLCLASEKDPDWVVDI
                     ATLTGAQVVALGTDIAGVMGNDDTFRSRVVDAADASGEGAWPMPLPVEMRAKLDTPTA
                     DLAHKGDRDGGMLTAGIFLKEFVGEGIPWAHVDIAGPAFNDKGPKGHTPKGGTGFGVA
                     TLLALVESSAG"
     gene            6125..>6731
                     /locus_tag="PL014_09930"
     CDS             6125..>6731
                     /locus_tag="PL014_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HOT57453.1"
                     /translation="MTELLHSPLHDQHVALGAKMADFGGWSMPIEYPGGGVVAEHTAV
                     RERVGIFDVSHLGKARVSGPGAADFVNACLTNDLRRIEPGQAQYTMCCTEEGGVVDDL
                     IQYLRSDDDVFLIPNAANTAAVVELLAAAAPEGITVENLHDRYGVIAVQGPKSDEVLA
                     GLGLPVDHDYMSFVETQWEGLPVIVCRTGYTGERGYEIVPAW"
BASE COUNT          915 a         2219 c         2541 g          956 t
ORIGIN      
        1 cccgagacga agcgcaccgg gtgttggacc tgtgcgatgg cggtggccag tgcgtgcgtg
       61 gtgtcggtgc gcgaggagag gatctcgcgc ttgtatgccg gggaccagcg cttgtcgccg
      121 actccggccc cggcgaggtg cacgacgccg tcgacgccgt cgagggcacg cgggtccagg
      181 gttcggctcg tcgggtccca ctgcacctcg tcacggcccc gggcagggcg gcgcacgaag
      241 cgcaccacct cgtcgccgcg ctgcgtgagc gcggcgacga cggcggtgcc gatcagcccc
      301 gaggagccag tgacggcgat gcgcttcatc tggtgctcca cccggtgccg gtcagtgctc
      361 gacgagacgt catcacagac ccaggtcggc ctcgaaggcg ccttcctcga gacgctgctt
      421 catcgtcgtg aggaagcggg cagcatccgc accatcgacg atgcggtggt cgtaggacag
      481 cgccaggtag accatcgagc ggatcgcgat cgtctcgcca ccgtcctcgg tcatggccac
      541 gacggggcgg cgaaccaccg agcccgtgcc gaggatcgcg acctgcggct ggttgatgat
      601 cggggtgtcg aacagcgccc ctcgcgagcc ggtgttggtg atcgtgaacg tgccaccgga
      661 caggtcgtca ggcatgatct tgttgttgcg cgtgcgctcg gcgacatcgg agatcccgcg
      721 agccaggccg gcgatgttga ggtcgccggc gttcttgagc accgggacga gcaggccctt
      781 ctcggtgtcg acagcgacgc cgaggtgctc ggcgccgtag tacgtcacct ggtcgccgtc
      841 gacgctggcg ttgaccgtcg ggtgggcctt gagggcctcg acggcggcga gcgcgaagaa
      901 cggcaggaag ctcagcttgg tgccctcacg agcggcgaag tctgccttgg cccggtcgcg
      961 cagcttggcg attcgggtga cgtcgacctc gacgaccgtg gtgagctgcg ccgagatctg
     1021 gagcgactcg accatgcggc gggcgatcgt ctggcgcagg cgggacatct tctcggtggt
     1081 gccccggcgc gagtcggtgg ccgcaggtgc cttggcgggc gcggcgggtg cggccgctgc
     1141 aggtgccggt gcctcggggg caggagccgg ggccttcttg gcctcggcgg ccgccatgac
     1201 gtcctgcttg cggatgcggc caccgatccc ggtgccggtc agcgtcgcga ggtcgatgcc
     1261 gtggtcggcg gccagcttgc gcacgagcgg cgtgacgtag gccgcggcat cgcggccctc
     1321 ggtgtcgtcg ctgctcgccg gtgcggctgc ggcaggagcc ggtgcctcgg cagcaggagc
     1381 aggagcggcg ggtgccgggg cgggagcagc cggggcggga gcagcggatg ccggggcggg
     1441 agctgccggg gcgggagctg cggatgccgg ggcgggagct gcggatgccg gggcgggagc
     1501 agcgggtgcc ggggcggcag cagccggctc gggagccgga gcctcagcgg cggcggcagg
     1561 agcggagcca ccaccggagc cgccgatgac cgccaggtca gcgccgacgg ggacggtgtc
     1621 gtcctcctgg acgaggatct tgttgagctt gccggccacg ggggagggga tctcggtgtc
     1681 gaccttgtcg gtcgagacct cgaggagcgg ctcgtcgacc tcgacgtcgt cgccctcctg
     1741 cttgagccag cgggtgacgg tgccctcggt gaccgactcg ccgagggccg gcatggtcac
     1801 ggtggtgccg tcgccgccgt cggaggacgt gtcggcagag ccctgaggtg cggcctgctc
     1861 ggccggtgca ctctccgcgg gggcgggggc gggggcggcc tcggcgggtg cggcctcggc
     1921 ttcgacgggt gcgggctcgg gcgcagcctc ggcgggtgcg gcctcgggct cgctcgcagc
     1981 gggcgcgtcg ccgtcaccga tgaccgcgag gtcagcgccg acggggacgg tgtcgtcctc
     2041 ctggaccagg atctcctgga gcgtgccggc gatgggcgaa gggatctcgg tgtcgacctt
     2101 gtcggtcgag acctcgagca gtggctcgtc gatctcgacg gtgtcgccga cccccttgag
     2161 ccagcgcgtc acggtccctt cggtgacgga ctcgccgagc gcaggcatct tcacgcgttc
     2221 tgacatcggg acagctctcc tttgtgggcg gtgacgttga ccactctagt tgtgtgcgtg
     2281 cagcggtttc ccagcgagcg ccagatgggc ctcgccgagg gcttcgttct gggtcgggtg
     2341 ggcgtgcagc agagccgcga cgtcctcggc gtgggcctcc cacgagacga tcaactgggc
     2401 ctccccgatc tgctcaccca tgcgtgaacc gaccatgtgg atgccgacga ccgggccgtc
     2461 cttgacccgc acgagcttga cgaagccctg ggtgcgcagg atctgggact tgccgttgcc
     2521 gccgaggttg tactcgtagg tctcgacccg gtcgccgtac tgctcggtgg cctgcgcctg
     2581 ggtcagcccc accgacgcga tctcgggttc gcagtaggtg acccggggga tggacgactc
     2641 cacgagaggc accggatcga gcccggcgat gtcctcggcc acgaagatgc cctgggcgaa
     2701 gccgcggtgg gcgagctgga gcccgggcac gatgtcgccg acggcgtaga ccccgtcgac
     2761 cgaggtacgg cagcgctcgt cggtgatgac gaatccgcgc tcgacgctca ccccggcatc
     2821 ctcgaatccc aacccggcgg tcgacggccc tcgcccgacg gcgacgagca gcacgtcggc
     2881 gctcagctcg tcaccaccct cgaggcgcac cgtgacaccg ctctcggtcg gggtggccga
     2941 ctcgaagcgc accccggtgc ggaaggcgat gccgcgcttc ttgaaggcgc gttccagcgc
     3001 ggtgctgacg gccgggtcct cggcggcgac gagccggggg agggcctcga cgatcgtcac
     3061 gtcggcgccg aacgagcgca tgacggaggc gaactcgacg ccgatgacac ccccgccgag
     3121 cacgacgatg ctcttgggca cctcggtcag cgcgagggcg ccgtcgctgg tcatgatcgg
     3181 gccgccgagc tcgagcccgg gcagggtccg ggcgtacgag ccggtggcca gcacgacgtg
     3241 gcggccgacg aggcgccggt cgccgacgac gacggtgcgc tcaccctcga ggcggccctc
     3301 gccctggacg tagtcgacct tgcgtgagga caccaggccc tgaaggccct tgtgcagccg
     3361 gctcacgacg ccctcgcggt aggcggtgac gcccggcagg tcgacaccgt cgagcgtcgc
     3421 gcgcaccccg aaacgggtgg cttcacgggc tgagtcggcg acctcggccg cgtgcagcag
     3481 cgccttggtg gggatgcagc cccggtgcag gcaggtgccg ccgaggaggt ccttctcgac
     3541 gagggcgacg ctcaatccga gctcggcggc gcgcagcgcg caggcgtagc cgccgctgcc
     3601 acctccgagg acgacgacgt catgggtggg ttcggcggtg gccggcatca gctgctcctt
     3661 cgcgcggggg tggggcgcct gcgagcatcc tgtcactccc tgagcgctta tcgtgtcgtc
     3721 atggcgtggt ggagacgcag tcgcgacaag caggcacccc atggcggtgg cgacctgagc
     3781 agcgcccgtg cccacctgca ggagttcgtc gggacgcggc ggggtgtgga ggcctacatc
     3841 gagcccgcga ccaacatcac cgcgacgacc gtggtcctca tcgcccacga cggggagtgg
     3901 acgaggcggg cactgccgtc ccggccggtg gccttcgagg tcgcccgctc gctcgacatc
     3961 cccgtctacg acgtgaacct caccggctac ccggcgcgca tgcgccagtg gaccgcccgc
     4021 cagcggcgcc ctgaccagcg ctgacggcag tgactcacgg cgccaccgac gccgggccag
     4081 ccgtcgtgga cggcaattcg atcgatcggg gcgctcgggt ctgcgaaggt tgacgcacca
     4141 cccgccgaac gaaggagcac catgtcaggc tcgaagaagc cgcgcgccac cgtcgacgag
     4201 gacgtgaagg ccaagttccg cgcagccctg gacgcgaaga agtcccacgg cggcgccgac
     4261 gtctcggacc actcggacca ctcgaaggtc gaccacgccc acggtccgga ggccagcgcg
     4321 acgcagcaga tgttccgccg caagagcggc ggctgannnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnctca gccggcgctg gactccacga gggccagcag ggtcgccacg
     4501 ccgaagccgg tgccgccctt cggggtgtgt cccttggggc ccttgtcgtt gaacgccggc
     4561 ccggcgatgt cgacgtgggc ccacgggatg ccctcgccga cgaactcctt gaggaagatc
     4621 ccggcggtca gcatcccgcc gtcacgatcg cccttgtggg ccaggtcggc ggtgggcgtg
     4681 tcgagcttcg cgcgcatctc gaccggcagc ggcatcggcc atgcgccctc acccgaggcg
     4741 tcggcggcgt cgacgacgcg ggagcggaag gtgtcgtcgt tgcccatgac acctgcgatg
     4801 tcggtgccca gcgccacgac ttgggcgccg gtcagggtgg cgatgtcgac cacccagtcg
     4861 gggtccttct cgctcgccag gcacagcccg tcgccgagga ccatgcgccc ttcagcgtcg
     4921 gtgtcgagga tctcgacgct cgtgccgttg cgcatgacga cgacgtcgct cggccgctgc
     4981 gcggtgccgc tcggcatgtt ctcggccagg cagaggtagc cggtgacggc cacgggcagg
     5041 gcgagctcgg ctgcggcgat gacggtggcc gcgacggcgg cggcaccggc catgtcgcac
     5101 ttcatcgtca ccatgcccga tgacggcttg atgttcaggc ctccggagtc gaaggtgatg
     5161 cccttgccga cgagggcgac cgaggccttc ggggaggccg ggctgtagga catcgtcacg
     5221 atgcgtggcg ggtgcaccga gccctgaccg acgccgacga tgccgccgaa cccgcccttg
     5281 acgagtgcct tgtcgtcgag cacggagacc gtgacgcgcg cggtcgaccc cgcgacgcgc
     5341 ttcttcacgg catccgcgaa ggaggcgggg tagaggtagt tcgggggagt gttgaccagg
     5401 tctcgggccc agtcgcgggc ggagccgagg atgccggcgc gcgtcacggc atccttggcg
     5461 gcacgcccct gcccggcacc ggacacgacg agcacccgcg ggccggatgc cgttgccccg
     5521 gtggtcgcgg tcggcttgcc ggggcgggcg ggcaccgtgc ggcgggccgc gccgaccacc
     5581 gcgaggggtg cgctcttgtc gtagacgtag cacccggcat acgccccgtc ggcgacggcg
     5641 gacaggctct cgacgtcggg ggtgggcagg gcgacggcga cggtgcgctt gccgcgcacg
     5701 gtgcgcaggg ccgctccggc agccgagcgc aggacggtgt gggggtaggt cgcccggcgg
     5761 gacgtgccct cgccgaggcc ggtgacgacc accgaggttg ccttgacgcc ggggacggcg
     5821 acgacccgta cgacctcgtc ggccttgccg gtggcctcga ggtcggcgag cacggcctgg
     5881 aggtggacga cggctttgcg gggcagggcg tgaccggtgg cgaggtcggt gccgtcggga
     5941 gttcgcacgg atccgatgac caggcagtcg acgggaagct cggcgggggt gcgggtggtg
     6001 agcgacacgg aagtcatgtc ggcaccctag cgaccacgtg ccgacgaccg ttggcacggt
     6061 tgagccgggt cgagcgtgct cgagcgcggg cgggcacggt cgggcacgcg gtaacgtgcc
     6121 gaccatgacc gagctgctgc actccccgct ccatgaccag cacgttgccc tcggcgccaa
     6181 gatggcggac ttcggggggt ggtcgatgcc gatcgagtac cccggcggcg gggtggtcgc
     6241 ggagcacacg gccgtgcgtg agcgagtcgg gatcttcgac gtgtcgcacc tgggcaaggc
     6301 gcgggtgtcc ggccccggtg cggcggactt cgtcaacgcg tgcctgacca acgacctgcg
     6361 ccgcatcgag cccggccagg cgcagtacac gatgtgctgc accgaggagg gcggggtcgt
     6421 cgacgacctc atccagtacc tgcgctcgga cgacgacgtc ttcctcatcc ccaacgcggc
     6481 gaacacggcg gccgtcgtcg agctgttggc cgcagccgcg cccgagggga tcacggtcga
     6541 gaacctgcac gaccgctacg gcgtcatcgc ggtgcagggg ccgaagtccg acgaggtgct
     6601 ggcggggctc gggctgcccg tcgaccacga ctacatgagc ttcgtggaga cccagtggga
     6661 ggggctgccc gtcatcgtct gccgcaccgg gtacacgggg gagcggggct acgagatcgt
     6721 gccggcgtgg g
//
LOCUS       DAOSOC010000134         6705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_11469_length_6705_cov_8.880602, whole genome shotgun sequence.
ACCESSION   DAOSOC010000134 DAOSOC010000000
VERSION     DAOSOC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11469_length_6705_cov_8.880602"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /gene="glyS"
                     /locus_tag="PL014_09935"
     CDS             complement(<1..645)
                     /gene="glyS"
                     /locus_tag="PL014_09935"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOT57454.1"
                     /translation="MAAPTSVVDTVVSLCKRRGFVFPCGEIYGGTRSAWDYGPLGVEL
                     KENIKRQWWRSMVTAREDVVGLDSSIILPRQVWVASGHVGAFTDPLTECQSCHKRFRV
                     DHMQEAVADREAKKGKEVDPDSIELTDLACPNCGTRGQWTPPREFNMMLKTHLGVLED
                     ESGLHYLRPETAQGIFVNFLNVMQASRKKPPFGIAQTGKSFRNEITPGNFIFRTR"
     gene            731..1015
                     /locus_tag="PL014_09940"
     CDS             731..1015
                     /locus_tag="PL014_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57455.1"
                     /translation="MSSLRESFERASLPALTYISSLPRFVPFLAILGLVIAGLLIPGW
                     GWVLIVLVVLLLAWIGALAWPRLSMPERMMRVAVVVMMAAIAITQAVPRG"
     gene            1058..2011
                     /locus_tag="PL014_09945"
     CDS             1058..2011
                     /locus_tag="PL014_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOT57456.1"
                     /translation="MKALAVAVLSSALVLAGCGDGGTDGASGTDGADRMPVTAAFYPL
                     QFVAERVGGDLVEVSTLTKPGTEPHDLELTPKAVAQLSQAKAVLYLAGFQPAVDDAVK
                     TQAADAALDVTQPANLIVTGADDGHDHAGESADEHAGHAADGVVDLHFWLDPTRMAAV
                     ATTVGERFAKADPANAATYAANAAALTSDLTTLDEEFAAGLKTCESKELVTGHAAFGY
                     LATRYGLGQEGIAGISPDAEPDAAALRDLVSHVRESGVSTVYAETLVSPALAETIARE
                     TGAKVAVLDPLEGLTDASPGSDYLEVMRANLKTLREGQGCS"
     gene            2008..2817
                     /locus_tag="PL014_09950"
     CDS             2008..2817
                     /locus_tag="PL014_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOT57457.1"
                     /translation="MTSPTLVRLRSASFGYADRTVVSGVDLTISSGEVVALLGPNGSG
                     KSTLVRGILGLSTRQGGDVELFDTPLDEFDQYTRLGYVPQRHSLSASVRATVTEIVEV
                     GRLPHRPWWKPASRQDQAIVREAIADVGLADRAQEEVAALSGGQQRRVLIARALAGRP
                     DMLVMDEPTAGVDHANQEALALVLQRLADRGTSMLVVTHELEALEGVVTRIVCLDAGH
                     IDFDGTPAAYADHLRRHAVGSDHHHPDDDSPRRGHVIGAAPLDPTPREVAP"
     gene            2814..3800
                     /locus_tag="PL014_09955"
     CDS             2814..3800
                     /locus_tag="PL014_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOT57458.1"
                     /translation="MSDLLALDFMRQALLAALLAGFAAPLVGVFLVQRRMSLIGDGMG
                     HVALAGVAVGVLTGQEPVLTALISAVLAAVAIELIRASGRTSGDTALAVMFYGGIALG
                     VVLISRSSSGTPANLTGYLFGAILTTSRADIIVFAVLAAVVLSATLVLRQRFFAVAND
                     EEYARAAGIPVLRYNLVLAVLTAVTVVVSMRVVGLLLISALMILPNATSQLFARSFRS
                     GLWWAVLIGVLSSVGGVLLSYAAETPSGGTIVLLAVAAFVTASVVTTVVARLRRAAHD
                     RAERHDHEHGPDCGHPAVEHDDHVDYLHDGHRHAAHEGHYDEHGQRAGVETR"
     gene            3797..4219
                     /locus_tag="PL014_09960"
     CDS             3797..4219
                     /locus_tag="PL014_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOT57459.1"
                     /translation="MTDQIQQIQTERRPTRQRAAIAEALDGSPEFRSAQDIHAAMAGS
                     GTKVGLATVYRNLQAMAAEGELDVIRTAEGEAVYRSCATAHHHHHVVCRSCGLAVEVT
                     GDAVERWAEAVAAANGFTQVRHTVEIDGLCAACSRASS"
     gene            complement(4209..5003)
                     /locus_tag="PL014_09965"
     CDS             complement(4209..5003)
                     /locus_tag="PL014_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOT57460.1"
                     /translation="MSAPTPYPPPFPHPSGARPPAIAPDRMPRHVAVVMDGNGRWANQ
                     RGLPRTKGHEAGEAALLDVVAGAIEVGVTHLSAYAFSTENWKRSPDEVKFLMGFNRDV
                     IRRRRDQLHEWGVRMRWVGRRPRLWGSVIKELEVAQELTRHNTGLTLYFCVNYGGRAE
                     LGDAVQRLAEDVAKGRLKARSVDERTIARYLPEPDMPDVDLFVRSSGEQRTSNFLLWQ
                     SAYAEMVFQNRLWPDYDRRDLWEAIQLYVDRERRYGGAIDLPETTS"
     gene            complement(5000..5725)
                     /gene="recO"
                     /locus_tag="PL014_09970"
     CDS             complement(5000..5725)
                     /gene="recO"
                     /locus_tag="PL014_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOT57461.1"
                     /translation="MPLYRDEAIVLRTQKLGEADRIVTLLTRRLGKVRVVGKGVRRTK
                     SRFGSRLEPGMHIDVQCYEGRNLDTVTQVETLDPWGDAIARDYPSYTAATAVLETCDR
                     LTEEREPALQQYLLLAGAVRSLAGREHDPNLVLDAYLLRALAVAGWAPSFHDCAKCGA
                     PGPHRGFHVVAGGAVCPSCRPPGSAAPAPETLLLLSALLAGDWAIADSSDLHHRREGS
                     GLTAAFLQWHIERGIRSLRMVER"
     gene            complement(5783..5974)
                     /locus_tag="PL014_09975"
     CDS             complement(5783..5974)
                     /locus_tag="PL014_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57462.1"
                     /translation="MTTVVTDSAGTAIAGLQAQPGGAAIAQAAGDAMIHASRLTTGLA
                     AIALVVGLLATFRLPRSAD"
     gene            6209..>6705
                     /locus_tag="PL014_09980"
     CDS             6209..>6705
                     /locus_tag="PL014_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57463.1"
                     /translation="MSDAPFFEPLPDPEELSEEEEQVVDLPWAPPSHIVGVIVPVDVD
                     VFRSDDVAVRVTHAACFGRGLELHVSAWVRPGSQPRQTDQRHHWRDHEPRVGVRLADG
                     TRLGHRHPHSPPPEEVEGAKGADEPTVSLTQMGGVGEMGRASSSWWLYPIPDGDSFEV
                     VVEWS"
BASE COUNT          941 a         2377 c         2391 g          996 t
ORIGIN      
        1 gcgggtgcgg aagatgaagt tgcccggggt gatctcgttg cggaagctct tgccggtctg
       61 ggcgatgccg aacggcggct tcttgcgcga ggcctgcatg acgttgagga agttcacgaa
      121 gatgccctgg gcggtctcgg ggcgcaggta gtgcagaccc gactcgtcct cgagaacgcc
      181 gaggtgcgtc ttgagcatca tgttgaactc gcggggtggg gtccactggc cgcgggtgcc
      241 gcagttgggg caggcgaggt cggtgagctc gatggagtcg gggtcgacct ccttgccctt
      301 cttggcctcg cggtcggcga cggcctcctg catgtggtcg acccggaacc gcttgtggca
      361 gctctggcac tcggtgagcg ggtcggtgaa ggcaccgacg tgccccgagg cgacccagac
      421 ctggcgcggc aggatgatcg aggagtcgag tccgacgacg tcttcgcggg cggtgaccat
      481 cgaacgccac cactggcgct tgatgttctc cttgagctcg acgccgagcg gcccgtagtc
      541 ccaggccgag cgcgtaccgc cgtagatctc gccacagggg aagacgaacc cccgacgctt
      601 gcagagggag acgacggtgt cgacgacgga ggtgggagcg gccatggcgg caaggctatc
      661 cggggaggtg ccccgcagcc gaaccggccc gtgcggggcc tgagcctcgc cgcgcgtagg
      721 ctggtgcacc gtgtccagcc ttcgtgagtc cttcgagcgc gccagcctcc cggcgctgac
      781 gtacatcagt tcgctcccgc ggttcgtgcc cttcctggcc atcctcggcc tggtcatcgc
      841 cggcctcctc atccccggct ggggctgggt gctcatcgtc ctcgtggtgc tcctgctcgc
      901 gtggatcggt gccctcgcgt ggccacgcct gagcatgccc gagcggatga tgcgggtggc
      961 agtcgtcgtc atgatggctg cgatcgccat cacccaggcg gttccgcgcg gctgacggtt
     1021 ttgacaatca ttctcaccat gattgagaat gattgtcatg aaggctcttg ctgttgctgt
     1081 cctgtcctcc gccctcgtgc tcgccgggtg tggtgacggc ggcaccgatg gggcgtcggg
     1141 caccgacgga gcagaccgga tgccggtcac ggccgccttc taccccctcc agttcgtcgc
     1201 ggagcgcgtc ggcggcgacc tcgtcgaggt gtcgacgctg accaagcccg gcaccgagcc
     1261 gcacgacctc gagctcacgc cgaaggccgt cgcccagctg tcgcaggcca aggccgtctt
     1321 gtacctcgcc ggcttccagc cggccgtcga cgatgccgtg aagacccagg ccgcggatgc
     1381 cgccctcgac gtcacgcagc ccgcgaacct catcgtcacc ggcgccgacg acggccacga
     1441 ccacgccggg gagagcgcgg acgagcacgc cgggcacgcc gcggacggcg tggtggacct
     1501 gcacttctgg ctcgacccga cccgcatggc cgcggtcgcg acgaccgtag gcgagcgctt
     1561 cgcgaaggcc gatccggcga acgcggcgac gtacgcggcc aacgcggccg ccctgacaag
     1621 cgacctgacc accctcgacg aggagttcgc cgccgggctg aagacctgcg agagcaagga
     1681 actcgtcacc gggcacgccg cgttcggcta cctcgccacc cgctacggcc tgggccagga
     1741 gggcatcgcc ggcatctctc ccgacgccga acccgacgcc gccgcgctgc gcgacctcgt
     1801 cagccacgtc cgcgagagcg gggtgagcac cgtctacgcc gagacgctcg tctcccccgc
     1861 gctcgccgag acgatcgccc gcgagaccgg cgccaaggtc gccgtgctcg accccctcga
     1921 aggactcacc gatgcctccc ccggctcgga ctaccttgag gtcatgcgcg ccaacctgaa
     1981 gaccctgcgc gagggccagg gctgttcatg accagcccca cgctcgtgcg actgcggtcg
     2041 gcctccttcg ggtacgccga ccgcacggtc gtgtccgggg tcgacctcac catcagctcg
     2101 ggcgaggtcg tggccctgct cggcccgaac gggtcgggca agtccacgct ggtgcgcggc
     2161 atcctcggcc tgagcacccg ccagggtggt gacgtcgagc tcttcgacac cccgctcgat
     2221 gagttcgacc agtacacccg gctcggctac gtgccgcagc gacactcgct gtcggcgtcc
     2281 gtgcgggcga cggtgaccga gatcgtcgag gtcggccgcc tcccccaccg gccgtggtgg
     2341 aagccggcat cccgccagga ccaggccatc gtgcgcgagg cgatcgccga cgtcggtctg
     2401 gccgatcgcg cccaggagga ggtcgccgca ctctccggcg gacagcagcg gcgcgtcctc
     2461 atcgcccggg cgctcgccgg gcgccccgac atgctcgtca tggacgagcc gaccgccggg
     2521 gtcgaccacg cgaaccagga ggcactggcg ctggtgctgc agcgactggc cgaccgggga
     2581 acctcgatgc tcgtcgtcac ccacgagctc gaggccctgg agggcgtcgt cacccgcatc
     2641 gtgtgtctgg atgccggcca catcgacttc gacggcaccc cggccgccta cgcggaccac
     2701 cttcgacggc acgccgtggg ctccgaccac caccacccgg acgacgactc cccccggcgc
     2761 ggacacgtca tcggggcggc cccgctggac ccgacccccc gggaggtggc gccgtgagcg
     2821 acctgctcgc cctcgacttc atgcgtcagg cgctgctcgc ggccttgctc gccgggttcg
     2881 cggcgcctct cgtcggggtg ttcctcgtcc aacgccggat gtcgctcatc ggtgacggca
     2941 tggggcacgt cgccctcgcg ggagtggcgg tcggtgtgct caccgggcag gagccggtgc
     3001 tgaccgcgct catctccgcg gtgctcgcgg cggtcgcgat cgagctgatc cgggcctcgg
     3061 ggcgcacgag cggtgacacc gccctcgcgg tgatgttcta cgggggcatc gcgctcggtg
     3121 tcgtgctcat ctcccgcagc agcagcggca ccccggccaa cctcaccgga tacctcttcg
     3181 gcgcgatcct cacgaccagc cgcgccgaca tcatcgtgtt cgccgtgctg gccgccgtgg
     3241 tgctgagcgc caccctcgtg ctgcgccaac ggttcttcgc cgtggccaac gacgaggagt
     3301 acgcgcgcgc ggccggcatc cccgtcctgc gctacaacct cgtgctcgcc gtgctcaccg
     3361 cggtcaccgt cgtcgtgtcg atgcgggtcg tcggcctgct gctcatcagc gcgctgatga
     3421 tcctgccgaa cgccacgtcg cagctgttcg cgcgcagctt ccgctccggg ctgtggtggg
     3481 cggtgctcat cggggtgctg agcagtgtcg gcggggtgct gttgtcctac gcggccgaga
     3541 cgccgtctgg cggcacgatc gtgctgctcg ccgtggctgc cttcgtgacg gcgagtgtcg
     3601 tgaccacggt ggtggcccgg ctgcgccggg cggcccacga ccgggccgag cggcacgacc
     3661 acgaacacgg gcccgactgc gggcaccccg cggtggagca cgacgaccac gtcgactacc
     3721 tgcacgacgg gcaccgccac gcggcgcacg aggggcacta cgacgaacac gggcaacgag
     3781 ccggggtgga gacacgatga ccgaccagat ccagcagatc cagaccgagc ggcgcccgac
     3841 ccgccaacgc gcagccatcg ccgaggccct cgacggctcc ccggagttcc gttcagccca
     3901 ggacatccat gcggccatgg ccgggtcggg caccaaggtg ggcctcgcga ccgtctaccg
     3961 caacctccag gccatggcag ccgagggcga gctcgacgtc atccgcaccg ccgagggcga
     4021 agccgtctac cgctcgtgtg cgacggcaca ccaccaccac cacgtcgtgt gccgctcgtg
     4081 cggtctcgcg gtcgaggtca ccggggacgc cgtcgagcgc tgggccgagg cggtcgccgc
     4141 ggccaacggg ttcacccagg tgcggcacac cgtcgagatc gacggcctct gcgccgcctg
     4201 ctcgagggct agctcgtagt ctctggtagg tcgatggcgc ctccatagcg acgctcgcga
     4261 tcgacgtaga gctggatggc ctcccacagg tcgcgccggt cgtagtcggg ccacagccgg
     4321 ttctggaaga ccatctcggc gtaggcgctc tgccagagca ggaagttgct cgtgcgctgc
     4381 tcccccgagc tgcgcacgaa caggtcgacg tcgggcatgt ccggctcggg caggtagcgg
     4441 gcgatggtgc gttcgtcgac cgatcgcgcc ttgagccgac ccttggcgac gtcctcggcg
     4501 aggcgctgca cggcatcccc gagctcggcg cgccccccgt agttgacgca gaagtacagg
     4561 gtgaggccgg tgttgtggcg agtgagctcc tgggccacct cgagctcctt gatcaccgat
     4621 ccccacaggc gcgggcggcg cccgacccag cgcatccgca ccccccactc gtgcagctgg
     4681 tcgcggcggc gacggatgac gtcgcggttg aagcccatga ggaacttcac ctcgtcgggg
     4741 ctgcgcttcc agttctccgt cgagaaggcg taggccgaca ggtgggtgac gccgacctcg
     4801 atggctccgg cgacgacgtc gagcagggcc gcctcgccgg cctcgtgacc cttggtgcgc
     4861 ggcaggcccc tctggttcgc ccagcgcccg ttgccgtcca tgacgacggc gacgtggcgc
     4921 ggcatccggt ccggtgcgat ggccgggggg cgggccccgc tcgggtgcgg gaacggcggc
     4981 gggtacgggg tgggcgcgct caccgctcca ccatacggag gcttcggatg ccgcgttcga
     5041 tgtgccactg caggaaggct gcggtcaggc cactgccctc acggcggtgg tgcaggtccg
     5101 aggagtcggc gatggcccag tcgccggcga gcagcgccga gagcagcagc agggtctcgg
     5161 gggccggggc ggccgatccc ggtggacgac agctcgggca caccgcccca cccgcgacga
     5221 cgtggaaccc gcggtgtggg ccgggtgcgc cgcacttggc gcagtcgtgg aagctcggcg
     5281 cccaacctgc gacggcgagc gcgcgcagca ggtaggcgtc gagcacgagg ttcgggtcgt
     5341 gctcgcggcc ggccagggaa cggaccgcgc cggcgagcag gaggtactgc tgaagggcgg
     5401 gttcgcgttc ctcggtgagc cggtcgcagg tctcgagcac ggcggtcgcg gcggtgtagc
     5461 tcgggtagtc gcgggcgatg gcatcccccc acgggtcgag ggtctcgacc tgggtcacgg
     5521 tgtcgaggtt gcgcccctcg tagcactgga cgtcgatgtg catgccgggc tcgagccggc
     5581 tgccgaagcg tgacttggtg cgccgcacgc cctttccgac gacccgcacc ttgcccaggc
     5641 gtcgggtgag gagggtgacg atgcggtcgg cctcacccag cttctgggtg cgcaggacga
     5701 tcgcctcgtc gcggtagagg ggcaccgcac cattgtcggt gctcgcggtg acatcgcggg
     5761 gatgccgtgc cgccgcacgt ggtcagtcgg ctgagcgcgg cagccggaac gtcgcgagca
     5821 gtcccaccac gagcgcgatg gcggccaggc cggtcgtgag ccggctggcg tggatcatcg
     5881 cgtcaccggc ggcctgggcg atggccgcac ccccgggctg ggcctgcaga ccggcgatcg
     5941 cggtgccggc cgagtcggtg accacggtcg tcacccggtc cacgacgtcg gccgggagcg
     6001 agagggccgc gaggttgctc tgcgtggtcc gccgaggctg gcgatgagga acccgccgag
     6061 cacggccact cccagggccc acccgagtgg gcgaggacat gatcgcagtc tggcaccggg
     6121 ggcgaacgcg caacagacct gggtcgacca tgtcgacagc cctcgtcgcg gctcaggact
     6181 cgcgagcgtc tctgacagga tctggcccat gagcgacgca ccgttcttcg agccactgcc
     6241 cgatcccgag gagttgtccg aggaggagga gcaggtcgtc gacctgccgt gggcaccgcc
     6301 gtcgcacatc gtcggggtga tcgtgcccgt ggatgtcgac gtgttccgca gcgacgacgt
     6361 cgccgtccgg gtgacgcacg ccgcctgctt cggacgcggg ctggagctgc acgtcagcgc
     6421 ctgggtgcga cccggcagcc agccacggca gaccgaccaa cgccaccact ggagggacca
     6481 tgagcctcga gtcggtgtgc ggctggccga cggcactcga ctgggtcacc ggcacccgca
     6541 ctcgcctccg cccgaggagg tcgagggcgc caagggcgcc gacgaaccca ccgtgtcact
     6601 cacgcagatg ggcggcgtcg gggagatggg ccgagcctcg tcatcctggt ggctgtaccc
     6661 catccccgac ggcgactcct tcgaggtcgt ggtcgagtgg tccca
//
LOCUS       DAOSOC010000135         6636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_11641_length_6636_cov_10.615712, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000135 DAOSOC010000000
VERSION     DAOSOC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11641_length_6636_cov_10.615712"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..829)
                     /locus_tag="PL014_09985"
                     /pseudo
     CDS             complement(1..829)
                     /locus_tag="PL014_09985"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
     gene            complement(906..2417)
                     /locus_tag="PL014_09990"
     CDS             complement(906..2417)
                     /locus_tag="PL014_09990"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOT57464.1"
                     /translation="MSPTPTVLDVAPSLRHPVDIRVVDHDDPLDGVEAVSVPVTTTDA
                     VPDVLGVDLAGLGRAGFRPTVGSALAFPASGAPVLVAVGVGDVASLTAATVRDAAAAF
                     ANAVPFDGHLATRVPRSSRMTTAESAAAIVEGVLLARYRFSLRSTEPETAEVASLTLV
                     APEGERAAVEEGAARGRLMARVGMLSRDLSTSPAGMLTATAMADLAVELGPDAGLEVE
                     VFDKEQLTEMGCGGLLGVNRGSVEPPRMIVMRYQPEGEPTGHLALVGKGIMYDSGGIN
                     LKPSDESHSQMKNDMTGAAAILAAMTALREVGCRARVTAYLMCTDNMPSGSAMQLGDV
                     LTARNGKTVEVKNTDAEGRLVMMDGLSLAVEDEADVIVDIATLTGACLRTFGTEVAGV
                     MGNHPAVVAQVRAAGERADEPAWELPLIRSYRSQLDSTIADMTNLGGPNAGSITAGLF
                     LEEFVHGTPWAHIDIAGTGQAESSRRWVQKGPTGFGSRLLVEFAASFTVPTDG"
     gene            complement(2467..2862)
                     /locus_tag="PL014_09995"
     CDS             complement(2467..2862)
                     /locus_tag="PL014_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOT57465.1"
                     /translation="MIRMVVRALVFLASAAVGLFVAAALVDGVRVGTSGLLTAIVVFA
                     VLQSVLAPFFAKVAARNAPAFLGGIGLLTTFVALFAANQLTKGLSISGGVGTWIAATV
                     VVWLATAVATLALPLLLAKAGLESRRTPA"
     gene            complement(2916..6323)
                     /locus_tag="PL014_10000"
     CDS             complement(2916..6323)
                     /locus_tag="PL014_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57466.1"
                     /translation="MTVTEKTAVRTATQNAGINLRLAQLGLPLPQGFEDTEAARLAAP
                     ILARQRELSRRLADRLCAADGRIQAFLDDYLADTGEQPQLPRRTLVLDEPGLARELSL
                     PFDGDTFTSPLLSSYRLVNGVLHNPANDRRTTAGVFHIAEGGLPIPDDKIAVPKAVFA
                     KLLGLAFQPPEVDMVLPYSANQPNPAACFVSLHLRPLVAPEVPGSVREKRMETRFIVP
                     GGTVANLDFVEGIFGNGGDPYLPENDASLAPETWTGHTGCVILAPHLIRVTKKELGLP
                     HVDDATERQQRDGMCWSSEDELYNGGKAFKICARDERGVIVTVIADTYFGYCKKEVKT
                     QISYSANLFGNAEEEHSGGALVFASYNLGQEYSGRYSGVADAVSVADVVARDPQRFTL
                     QPQGHAVDGEQPHIVIVPAQARFSMKDMTVTWGEANGIPLRADTVYLSPSGYRVTVAQ
                     QPHDPSTWTLVGTAATATSCHKPATVSGGGKSEISKAITDAFIVGSVFVADVDADLDA
                     VAAILDRDLTDRFLDPAQRGTDHRPILSPERSIGSVIKLLTPSPSTYTQEYNAWLRTI
                     PPYVKELVYVVKRSYQPQWGQDWRSHFSVAEIDGRTGNSLHLDGTKITLNMLRVGFDT
                     DGRWRLFSLRHDFAPAVKVQTQDDITASTVVPGDQLGLDPNRSYKLVENCENLLFQRP
                     DDAIHRGYDSVAERDIATPGTFLSNFAPLTHADAVAMRDDAVSFTAFTEPMATLIREF
                     ADTPEGAGPDFVVSSANPRIVDGKPSKNPRYLQQRPDLTNAKATAVAHLSERLAAGMP
                     ASAPLPRPVDVVAAGRRNNPPEAGVPALCSYGPLHYMEVPELFMEFIASMTGKSPSTT
                     GAGSEGALTKGPFNALPTIIDLNANLLSYVLTGHDGWLSAAGFVGPHVRVDHDVSLLV
                     PELFTRMTPQERDARNLIAEGALEKVEDVEHDGRRVPASRLGYRMTETMATKYFGRVF
                     LHPEVVFTPEMLRPELQDPAVFAESVTTIAMTHERVARAFVKDGTIDLAIPPLRALLE
                     IMADGVTASGLTLDSPEFREMFTRESVLASDWYAERLDAKQDAARSRADAGLAAIEKF
                     VSTPGNEEPTQRLGMPGRVDAARAESQRLAGPEFRDMLVGTTGSTPL"
BASE COUNT          969 a         2213 c         2485 g          969 t
ORIGIN      
        1 gcgaccgggg tcatcacgag tgccccgacg aacgcggaca gcaccccgcc gaggaggagc
       61 ccgctgagca cctgcgcggc gtacgcgacg tagaggacga ggatgatcca tgcggcggac
      121 cgcggccgtg cgcactcgtg caccaccacg ccgaccccga acaggaccac gccgatccac
      181 ggtgcccagg ccggcaccgg atccgccgcc agctgggtga ccgacacggt gggcaccccc
      241 gcgacggcgg cagcaccgac gattcccgcc gcgagcagga ccagcctcat cacccccgcg
      301 ctgaggcggc ccgacccgga gatcatctgc ccggtcgaca gctcgatgac cgcggtcgtc
      361 agcagcgccc cgggcaggaa gatcacgaga ggggcgatga gtggggtcgt caccgagagt
      421 tcggggagca cgtcgacgag caggaagacc gccagggcca cgccgaaggc agcggccacc
      481 ggaaggaaca ccccgaagga tggggcgaac cgtcgcaccg cgacctcgac gactcccacg
      541 agcaggccga gaccggtcgc caccgcgacg tcgacccagc tcccgcgcag gatgagggcg
      601 atcccgcctc ggcgtcgtcg acgaggcgga agaggtcgtc gacctggtcc agtcggagcg
      661 ggcgcccacc ggcggaggcc actgccgtgt gcgtggacgt ggacccgggc accgacacga
      721 acagcgccgt ggccaggacg acgacctcgg tgccgacggc gcccagtgcg agcgccaccc
      781 gctcgaggct ggcacgcacg tgggtcaccg ggtcgccggc atcgaccatg gcctcgccga
      841 gggcgacgag gaagtcggcg atgtcggtct ccgcggccgg ttcgggggcc gcccggctca
      901 cgtcatcaac cgtcggtggg gacggtgaac gaggccgcga actcgacgag caggcgggac
      961 ccgaagccgg tggggccctt ctgcacccag cgccgcgagg actccgcctg gcccgtgccg
     1021 gcgatgtcga tgtgcgccca cggggtgccg tggacgaact cctcgagaaa caggccggcg
     1081 gtgatcgacc cggcgttcgg cccaccgagg ttggtcatgt ccgcgatcgt ggagtcgagc
     1141 tgtgacctgt aggacctgat gagcggcagc tcccacgcgg gttcgtcggc ccgctcgccc
     1201 gcggcccgga cctgcgcgac gacggcgggg tggttgccca tgaccccggc gacctcggtg
     1261 ccgaaggtgc gcaggcaggc tcctgtgagg gtggcgatgt cgacgatgac gtccgcctcg
     1321 tcctccaccg ccagggacag cccgtccatc atcaccaggc gcccttcggc atcggtgttc
     1381 ttcacctcga cggtcttgcc gttgcgggcg gtgagcacgt caccgagctg catggccgag
     1441 ccggacggca tgttgtcagt gcacatgagg tatgccgtga cgcgggcccg gcagcccacc
     1501 tcgcgcaggg cggtcatcgc cgcgaggatg gcggccgccc cggtcatgtc gttcttcatc
     1561 tgcgagtgcg actcgtcgct cggcttgagg ttgatgccgc cggagtcgta catgatgccc
     1621 ttgccgacca gggccaggtg ccccgtgggc tcgccctcgg gctggtagcg catcacgatc
     1681 atgcggggtg gctcgacgct gccgcggttg actccgagca ggccaccgca gcccatctcg
     1741 gtgagttgtt ccttgtcgaa cacctcgacc tcgagcccgg cgtcgggccc gagctcgacg
     1801 gcgaggtcag ccatggcggt cgcggtgagc atcccggcag ggctggtgga caggtcccgg
     1861 ctgagcatgc cgaccctggc catgaggcgg cctcgggcgg cgccctcctc gaccgcggcc
     1921 cgctcgccct cgggcgccac gagggtcagg gaggcgacct cggccgtctc cggctcggtg
     1981 ctgcgcaggc tgaagcggta gcgcgcgagc agcacgccct cgacgatggc ggcggcggac
     2041 tcggcggtcg tcatccgcga cgagcgcggc acgcgggtcg cgaggtggcc gtcgaagggc
     2101 accgcgttcg cgaaggccgc cgcggcgtcg cgcacggtgg cggccgtcag ggaggccacg
     2161 tcaccgacgc ctacggccac gagcaccggt gcgccggagg cggggaacgc gagcgcggac
     2221 ccgaccgtgg gacggaaacc cgcgcgcccc aggccggcga ggtcgacgcc gaggacgtcg
     2281 gggacggcgt ccgtcgtcgt caccgggacg ctcaccgcct cgacgccgtc gagggggtcg
     2341 tcgtggtcga cgacacgaat gtcgacgggg tggcgcaggg acggggcgac gtcgaggacg
     2401 gtgggtgtcg ggctcatggc gccgacgcta gtccgcagcc gctgacgagg agggaaaacg
     2461 gcgctctcag gccggggttc ggcgtgactc caggccggcc ttcgcgagca gcagcggcag
     2521 cgcgagagtc gcgaccgcgg tggccagcca cacgacgacg gtcgcggcga tccacgtacc
     2581 gaccccaccg ctgatgctca ggcccttggt cagctggttg gcggcgaaca gcgcgacgaa
     2641 cgtcgtgagc aggccgatgc cgccgaggaa ggccggagcg ttgcgtgcgg ccaccttggc
     2701 gaagaacggg gccagcacgc tctggagcac ggcgaagacg acgatggccg tgaggagccc
     2761 gctcgtgccg acccggaccc cgtcgaccag ggctgcggcc acgaacagtc cgacggccgc
     2821 ggacgcgagg aacaccaggg cacgaacgac catccggatc atggagggat cctagcggcg
     2881 ccctcggtgg cgctcgtcgt cgtcgcccgt gtcgcctaga gcggcgtgct ccccgtcgtc
     2941 ccgacgagca tgtcgcggaa ctccggcccg gcgaggcgct gggactccgc gcgcgcggcg
     3001 tcgacgcgcc ccggcatccc gagtcgttgc gtcggctcct cgttgcccgg cgtcgagacg
     3061 aacttctcga tcgccgcgag acccgcatcg gcgcgcgacc gggcggcatc ctgcttggcg
     3121 tccagacgct cggcgtacca gtcggaggcc agcaccgact cccgggtgaa catctcccgg
     3181 aactcggggg agtccagggt cagccccgac gcggtgacgc cgtcggccat gatctcgagc
     3241 agtgcccgca gtggcgggat cgcgaggtcg atggtgccgt ccttgacgaa ggcccgcgcc
     3301 acccgctcgt gggtcatcgc gatggtcgtc accgactccg cgaacacggc cgggtcctgc
     3361 agctcggggc gcagcatctc gggggtgaac acgacctcgg ggtgcaggaa gacgcggccg
     3421 aagtacttcg tcgccatcgt ctcggtcatc cggtagccca ggcggctcgc aggcacgcgc
     3481 cgaccgtcgt gctcgacgtc ctcgaccttc tccagcgcgc cctcggcgat gaggttgcgc
     3541 gcgtcgcgct cctgcggggt catccgggtg aacagctcgg gcacgaggag cgagacgtcg
     3601 tggtcgaccc gcacgtgcgg cccgacgaac ccggccgcgg acagccagcc gtcgtgcccg
     3661 gtcagcacgt agctgagcag gttggcgttg aggtcgatga tcgtcggcag cgcgttgaac
     3721 gggcccttgg tcagggcccc ctcggagccg gcgccggtcg tcgacggcga cttgcccgtc
     3781 atcgacgcga tgaactccat gaagagctcg ggcacctcca tgtagtgcag cggtccgtac
     3841 gagcacagcg cggggacgcc ggcctcgggc gggttgttgc gccgaccggc cgcgacgacg
     3901 tccactggcc ggggaagggg ggcactcgcc ggcatccccg ccgccaggcg ctcgctgagg
     3961 tgcgccaccg cggtggcctt cgcgttggtg aggtcgggcc gctgctggag gtagcgcggg
     4021 ttcttcgacg gcttgccgtc gacgatgcgc ggattcgccg agctgacgac gaagtcgggc
     4081 ccggccccct cgggggtgtc ggcgaactcg cggatgaggg tggccatcgg ctcggtgaac
     4141 gccgtgaagc tgaccgcgtc gtcacgcatc gccacggcat ccgcgtgggt gaggggggcg
     4201 aagttggaca ggaacgtgcc gggggtcgcg atgtcccgct cggccaccga gtcgtacccg
     4261 cggtggatgg cgtcgtcggg tcgctggaag agcaggttct cgcagttctc gacgagcttg
     4321 tacgagcgat tcgggtccag gcccaactgg tcgccgggaa cgaccgtgga tgccgtgatg
     4381 tcgtcctgcg tctgcacctt caccgcgggc gcgaagtcgt gacgcaggct gaacaggcgc
     4441 caccggccgt cggtgtcgaa gcccacccgc agcatgttga gggtgatctt cgtgccgtcc
     4501 aggtgcagcg agttgccggt gcggccgtcg atctcggcga cgctgaagtg gctgcgccag
     4561 tcctgacccc actgcggctg gtacgagcgc ttgacgacgt agacgagttc cttgacgtac
     4621 ggcgggatcg tgcgcagcca ggcgttgtac tcctgcgtgt acgtcgaggg cgagggcgtc
     4681 agcagcttga tgaccgaccc gatcgagcgc tccggtgaca ggatcggccg gtggtcggtg
     4741 ccccgctggg cggggtcgag gaagcggtcg gtgaggtcgc gatcgaggat ggccgcgacg
     4801 gcatccaggt cggcgtcgac gtcggcgacg aagaccgagc cgacgatgaa cgcgtcggtg
     4861 atggccttgg agatctcgga cttgcctccg ccggagaccg tcgccggctt gtggcacgac
     4921 gtggcggtcg cggcggtgcc gacgagggtc cacgtgctgg ggtcgtgggg ttgctgagcg
     4981 acggtgaccc ggtagccgct cgggctgagg tacacggtgt cggcacgcag ggggatgccg
     5041 ttcgcctcgc cccaggtgac cgtcatgtcc ttcatcgaga agcgcgcctg cgccggcacg
     5101 atgacgatgt gcggctgctc gccgtcgacg gcgtgaccct gcggctggag ggtgaagcgc
     5161 tgcgggtcgc gcgcgacgac gtcggcgacg ctcacggcat ccgcgacgcc tgagtacctg
     5221 ccggagtact cctgaccgag gttgtagctc gcgaagacca gcgccccgcc ggagtgctcc
     5281 tcctcggcat tgccgaacag gttcgcggag taggagatct gcgtcttgac ctccttcttg
     5341 cagtacccga agtaggtgtc ggcgatgacg gtgacgatga cgccgcgctc gtcacgggcg
     5401 cagatcttga aggccttgcc cccgttgtag agctcgtcct cgctcgacca gcacatgccg
     5461 tcacgctgct ggcgctcggt ggcgtcgtcg acgtgcggca ggcccagttc cttcttcgtg
     5521 acgcggatca ggtgcggggc gaggatgacg cagccggtgt ggccggtcca cgtctcgggc
     5581 gccagcgagg cgtcgttctc cgggaggtag gggtcgccgc cgttgccgaa gatcccttcg
     5641 acgaagtcga ggttcgcgac cgtgccgccg ggcacgatga agcgcgtctc catccgcttc
     5701 tcgcgcaccg accccggcac ctccggggcg acgagcgggc gcaggtgcag cgagacgaag
     5761 caggcggccg ggttcggctg gttcgccgag tagggcagca ccatgtcgac ctcgggcggc
     5821 tggaaggcca ggccgagcag cttcgcgaag acggccttcg gcacggcgat cttgtcgtcc
     5881 gggatcggca ggccgccctc ggcgatgtgg aagaccccgg ccgtggtgcg ccggtcgttg
     5941 gccgggttgt gcagcacccc gttgacgagc cggtagctgc tcagcagggg tgaggtgaag
     6001 gtgtcgccgt cgaagggcag ggacagctcg cgggccaggc ccggctcgtc cagcacgagg
     6061 gttcgccgcg gcagctgggg ctgctcgccg gtgtcggcga ggtagtcgtc gaggaaggcc
     6121 tggatgcggc cgtcggcggc gcacaggcgg tcggccaggc gccggctgag ctcgcgctgg
     6181 cgggccagga tcggtgctgc caggcgcgcg gcctcggtgt cctcgaaccc ctgcggcagg
     6241 gggaggccga gctgggcgag ccgcaggttg atgccggcgt tctgcgtggc ggttcggacg
     6301 gcggtcttct cggtgacagt cacgcaacca gtctgtccct gccggggtcg cgtcgccatt
     6361 caggaaagcc tcatcaactt tcccgcccga tgccacaagg ttcaccttcg ccggggttca
     6421 ttcccccggc gatcatgaac cttctcgtgt cgagctcagc cgggatgctg acggtggggg
     6481 ccgtcgctcg ccctgcagcg tcggttctgg cgggtatctc gaagccggcg caagggccgt
     6541 ggcaggggtg tggtgagggt ctggaagagg gttcgaaaat tgtttgaaag tcgttgtgcc
     6601 acagctgctt tcgtggctca ctgggcttgt tgatcg
//
LOCUS       DAOSOC010000136         6624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_11679_length_6624_cov_9.538438, whole genome shotgun sequence.
ACCESSION   DAOSOC010000136 DAOSOC010000000
VERSION     DAOSOC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11679_length_6624_cov_9.538438"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..799)
                     /locus_tag="PL014_10005"
     CDS             complement(98..799)
                     /locus_tag="PL014_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT57467.1"
                     /translation="MRVVIAGAGSVGRSIARELLRNEHHVLLIDRDADGERVAKVPDA
                     SWLQADACEIEALSEAGLADCEVVVAATGDDKVNLVLSLLAKTEFGVPRTVARVNNPK
                     NEWMFDEAWGVDVAVSTPRIMTALVEEAVSVGDLVRIFEFQQGKAMMVEMTLPVGSPY
                     AGSRVGDVPLPGNTVLTGIIREGHPIAPTRDDALEPLDELLFLTTPDVEAELETLLSP
                     GQRHKRDSEDPDDER"
     gene            complement(818..1480)
                     /locus_tag="PL014_10010"
     CDS             complement(818..1480)
                     /locus_tag="PL014_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT57468.1"
                     /translation="MHFVIMGCGRVGASLARAIERAGHEVAVIDQDESSFRRLGTTFE
                     GRTVTGVGFDRDTLRAAGIENAYAFAAVSSGDNSNILAARVAREKYGVEHVVARIYDP
                     GRAEIYQRLGIPTVATVKWTSDQMVRRLLPQGHVPEFTDPSGKVVIAEMPISDAWIGR
                     RISEIEGATGGRTAYLTRLGDGLLPGPDTVYQEGDLLHLALLREDLATAERVLDAAPP
                     AH"
     gene            1571..>2823
                     /locus_tag="PL014_10015"
     CDS             1571..>2823
                     /locus_tag="PL014_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOT57469.1"
                     /translation="MPNSGRVVKRVILGRAMRSDNLGETLLPKRLALPVFASDALSSV
                     AYAPDEIFLTLGLAGGALALTHSWEIALAVVVVMAVVIASYRQNVRAYPSGGGDYEVA
                     SVNLGPKAGLGVGSALLVDYVLTVAVSISSGVQNAAAAFGFVRGNEVLVAILLIAALT
                     AMNLRGVRESGTAFAIPTYLFMATVLGMALIGVVRKMTGSLPQAESAGLELLPDPSHD
                     AITGLALAFLFLRAFSSGCAALTGVEAISNGVPAFRKPKSQNAATTLLLMGMISVTMM
                     LSMVALATWTGVKYAEDPAVQLARNGVLVGEDYEQHTVMVQVAQSVFSGFHAGVVLVS
                     IVTGLILVLAANTAFNGFPVLGSILARDGFLPRQLHTRGDRLAFSNGIIILAAAAALL
                     VAIYDAKVTALIQLYIVGVFVSFTLS"
     assembly_gap    2824..2923
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2924..3419
                     /locus_tag="PL014_10020"
     CDS             <2924..3419
                     /locus_tag="PL014_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOT57470.1"
                     /translation="DTKAQMLPSRVHAIVLVSKIHKPTLRAVAYARATRPTILEALTV
                     DVDPDETKALQAEWVRRDIPVPLKALDSPFREITRPVVDYVKSIRSGNPRDLVVVYIP
                     QYVVGHWWEQILHNQSALRLRSRLLFTPGVMVSSVPWQLASSEGMEERLDGPAAGDVR
                     RGEG"
     gene            3419..4720
                     /locus_tag="PL014_10025"
     CDS             3419..4720
                     /locus_tag="PL014_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOT57471.1"
                     /translation="MAAHDTAHASSLAVGDEVEVDVTTIAHGGHCIARHEGQVLFVRH
                     ALPGERVLARVTETGAGERFVRADAVAMLSASPDRVTPPCPWSGPGRCGGCDLQHVSL
                     PRQRRLKADVVREQFSRLARLDVDVTVEPVPGDDAGLDWRTRVEFAVGADGRAGLRRH
                     RSHEIVAVDHCRIAAPGVDRLRVTGRTWPGTDAVDAVAPSVGDAVAVVVPGGEAPLVR
                     ERVVVGAGGEREFTLSARGFWQVHPGAAQTFVDAVLEAAAPRAGERALDLYAGVGLFA
                     AALARAVGPSGQVIAIESDAAACAHARDNLADLPQVAVLAARVDDAFGVPRPSRRGSS
                     SQRGSRPRKPSRSTLVPASADVVVLDPPRTGAGKGVCAEIAAMAPRAVVYVACDPAAL
                     ARDTAYFADLGYRLDTLRAFDAFPMTHHVECVALLTKSGPA"
     gene            4723..5697
                     /locus_tag="PL014_10030"
     CDS             4723..5697
                     /locus_tag="PL014_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HOT57472.1"
                     /translation="MPEDFVVARNTEPGSTLPYLLRIPLGDGVMLKAKDTWPRTGKVY
                     CHRLDEWPADAEIIERVPVRSCVRRGAAIDLVLDRGRENRSQLVITTARGRQMIFWQT
                     SRTAKQARPAVAVPGARASGLADVEISVDIRERYPFTFADRQVTTRREPLRAGDYGLV
                     VDGVLQASVERKSLTDLVSSLTTGTLKFQLTELSAIPRAAVVVEDRYSRVFALEHVRP
                     SVVADGIAECHIRFPSVPIVFCETRKLAQEWTYRFLAAARVGLAEEVVGDLAVRGLEA
                     APPLAPPPPTPAQVRRWAVAHDIAVSDRGRIAGSVLERYLEARARGEL"
     gene            6119..>6624
                     /locus_tag="PL014_10035"
     CDS             6119..>6624
                     /locus_tag="PL014_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOT57473.1"
                     /translation="MASTNSFDAHGTLEVGEQSYEIYRLAAVEGSDTLPYSLKVLLEN
                     LLRTEDGANITAEHIRALAGWDENAEPDTEIQFTPARVIMQDFTGVPCVVDLATMREA
                     VADLGGDPSKINPLAPAELVIDHSVQIDVFGRSDAFERNVEFEYGRNFERYQFLRWGQ
                     TAFDDFKVV"
BASE COUNT          878 a         2318 c         2310 g         1018 t
ORIGIN      
        1 cgatcgcgat catggggtgg atgctcgtgg ccgggcacac cccgctggag cctgagccgg
       61 aggtcgtccg cgatcaggag gccgaccagg cctgagctca gcgctcgtcg tcggggtcct
      121 cggagtcgcg cttgtgccgc tggccggggc tgagcagggt ctcgagctcg gcctcgacgt
      181 caggggtggt gaggaagagc agctcatcga gcggctcgag cgcgtcgtcg cgggtggggg
      241 cgatcgggtg gccctcgcgg atgatgccgg tgagcacggt gttgcccggc agcggcacgt
      301 ccccgacgcg cgagccggcg taggggctgc ccacgggcag ggtcatctcg accatcatcg
      361 ccttgccctg ctggaactcg aagatccgca cgaggtcacc gacgctcacg gcctcctcga
      421 ccagggcggt catgatccgc ggggtcgaga ccgcgacgtc gacgccccag gcctcgtcga
      481 acatccactc gttcttcggg ttgttgaccc gggcgaccgt gcgcggcacg ccgaactcgg
      541 tcttggcgag cagggagagc acgaggttga ccttgtcgtc accggtggcg gccacgacga
      601 cctcgcagtc ggccaggccc gcctcgctga gcgcctcgat ctcgcaggcg tcggcctgca
      661 gccagctggc gtcgggcacc ttggccaccc gctcgccgtc ggcgtcgcgg tcgatgagga
      721 gcacgtggtg ctcgttgcgc agcagctcgc gggcgatcga acgcccgacg ctgccggctc
      781 cggcgatgac gacgcgcatg ggtgacgggc ctttcggtca gtgggccggg ggagcggcgt
      841 cgagcacgcg ctccgcggtg gcgaggtcct cacgcagcag cgcgaggtgg aggaggtcgc
      901 cctcctggta cacggtgtcc ggcccgggca gcaggccgtc gccgagccgg gtcagatagg
      961 cggtgcgccc acccgtcgcg ccctcgatct cgctgatccg tcggccgatc cacgcgtcgg
     1021 agatcggcat ctcggcgatg acgaccttgc cggaggggtc ggtgaactca ggcacgtgtc
     1081 cctgcgggag caggcggcgg accatctggt cgctcgtcca cttgacggtg gcgacggtgg
     1141 ggatgccgag ccgctggtag atctcggcgc gaccggggtc gtagatgcgg gcgaccacgt
     1201 gctcgacgcc gtacttctcg cgcgccaccc gggcggcgag gatgttggag ttgtcaccgc
     1261 tggagacagc ggcgaaggcg taggcgttct cgatgccggc cgcgcgcagg gtgtcgcggt
     1321 cgaagccgac cccggtgacc gtgcggccct cgaaggtcgt gcccaaccga cggaacgacg
     1381 actcgtcctg gtcgatgacg gcgacctcgt ggccggcccg ctcgatggct ctggcgaggg
     1441 aggccccgac gcggccgcag cccatgatga cgaagtgcac ggagtcgacg ctacaccgac
     1501 gggccacgcg cggtgagggc gtcccgcaag ggttcccggg gaggtgggcg accggcatac
     1561 agtgtgtgtc gtgccaaatt ctgggcgcgt ggtcaagcgc gtcatcctcg gtcgcgcgat
     1621 gcgcagcgac aacctcggtg agacgctcct tcccaagcgg ctcgccctgc ccgtcttcgc
     1681 cagtgacgct ctgtcgtcgg tggcgtacgc ccctgacgag atcttcctga cgctcggcct
     1741 ggccggcggc gcgctcgcgc tcacccactc gtgggagatc gccctggcgg tcgtcgtcgt
     1801 catggccgtg gtcatcgcga gctaccggca gaacgtgcgc gcctacccct cgggcggcgg
     1861 cgactacgag gtggccagtg tcaacctcgg gcccaaggcc gggctcgggg tcggcagcgc
     1921 cctgctcgtc gactacgtgc tgacggtggc cgtctcgatc tcctccggcg tccagaacgc
     1981 cgcggcagca ttcgggttcg tgcgcgggaa cgaagttctc gttgccatcc tcctcatcgc
     2041 ggccctgacc gcgatgaacc tgcggggggt ccgcgagtcg ggcacggcgt tcgcgatccc
     2101 gacctacctg ttcatggcga ccgtgctcgg catggcgctc atcggggtgg tccgcaagat
     2161 gaccggcagc ctgccccagg ccgagagtgc cgggctcgag ctgctgcctg atcccagcca
     2221 cgatgccatc accgggctgg ccctggcctt cctgttcctg cgcgcgttct cctctgggtg
     2281 cgccgccctc accggggtcg aggcgatcag caacggggtg ccggcgttcc gcaagcccaa
     2341 gagccagaac gcggcgacga cgctgctgct catgggcatg atctcggtga cgatgatgct
     2401 ctcgatggtc gcactcgcga cgtggaccgg ggtgaagtac gccgaggacc cggccgtcca
     2461 gctcgctcgc aacggggtgc tcgtcggtga ggactacgag cagcacaccg tgatggtgca
     2521 ggtggcccag tcggtcttct ccggcttcca cgccggggtg gtgctcgtct cgatcgtcac
     2581 cgggttgatc ctcgtgctgg ccgcgaacac cgcgttcaac ggcttcccgg tgctgggctc
     2641 gatcctcgcc cgcgacggct tcctgcctcg ccagctgcac acccggggcg accggctcgc
     2701 gttctccaac ggcatcatca tcctggccgc cgccgcggcg ctgctcgtcg ccatctacga
     2761 cgccaaggtc accgccctca tccagctcta catcgtcggg gtgttcgtgt cgttcaccct
     2821 gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgacacc aaggcgcaga
     2941 tgctgccgtc acgggtgcac gccatcgtgc tcgtctccaa gatccacaag ccgaccctgc
     3001 gggccgtcgc ctacgccagg gccacccgcc cgacgatcct cgaggcactg accgtcgacg
     3061 tcgaccccga cgagaccaag gcgctgcagg ccgagtgggt gcgccgcgac atcccggtgc
     3121 cgctgaaggc gctggacagc ccgttccgcg agatcacccg ccccgtggtc gactacgtga
     3181 agtcgatccg ctcgggcaac ccgcgcgacc tcgtcgtcgt ctacatcccg cagtacgtcg
     3241 tcggccactg gtgggagcag atcctgcaca accagagcgc cctgcgcctg cgctcgcgac
     3301 tgctgttcac cccgggcgtc atggtgtcgt cggtgccgtg gcagctggcg tcgtcggagg
     3361 gcatggagga acggctcgac ggtccggcgg cgggcgacgt gcgccgaggt gagggctgat
     3421 ggccgcccac gacaccgctc acgcgagcag cctcgccgtc ggggacgagg tcgaggtcga
     3481 cgtcacgacg atcgcccacg gcggccactg catcgcccgc cacgaggggc aggtgctgtt
     3541 cgttcggcac gccctgcccg gcgagcgggt gctcgcccgg gtgaccgaga cgggtgccgg
     3601 agagcgtttc gttcgggcgg atgccgtcgc gatgctctcg gcgtcaccgg accgcgtcac
     3661 acccccgtgc ccgtggtcgg ggccgggccg ctgcggtggg tgcgacctcc agcacgtgtc
     3721 gctcccgcgg cagcgccggc tcaaggctga cgtggtgcgc gagcagttct cccggctggc
     3781 ccgtctcgac gtcgacgtca cggtcgaacc cgtgcccggg gatgacgcag gcctggactg
     3841 gcgcacccgc gtcgagttcg cggtcggtgc cgacggccgc gcagggcttc gccgccaccg
     3901 ctcgcacgag atcgtcgccg tcgaccactg ccgcatcgcc gcgccgggcg tcgaccgcct
     3961 gcgggtcacc ggacgcacgt ggccgggcac ggacgccgtg gatgccgtcg cgccgtcggt
     4021 gggcgacgcc gtggccgtcg tcgtccccgg cggggaggcc ccgctcgtgc gcgagcgcgt
     4081 ggtcgtcggt gccggcggcg agcgcgagtt caccctctcc gctcgcggct tctggcaggt
     4141 gcaccccggt gcggcgcaga ccttcgtgga tgccgtgctc gaggctgccg cgccacgcgc
     4201 cggggagcgg gccctcgacc tctacgcggg cgtcggactc ttcgcggccg ccctcgcccg
     4261 ggcggtcggc ccgagcgggc aggtcatcgc catcgagtcc gatgcggcgg cctgcgcgca
     4321 tgctcgcgac aacctcgccg acctcccgca ggtcgcggtg ctcgccgccc gggtcgacga
     4381 cgcgttcggg gtgcctcgtc cgtcgcggcg cggcagttct tcgcagcggg ggtcacgccc
     4441 acgcaagccg tcgcgctcga ccctcgtccc ggcatccgcc gacgtcgtcg ttctcgaccc
     4501 gccgcgaacg ggcgcgggca agggcgtgtg tgccgagatc gccgcgatgg ccccgcgtgc
     4561 ggtggtatac gtcgcgtgcg acccggccgc gctggcgcgc gacacggcct acttcgccga
     4621 cctcggctac cgcctcgaca cgctgcgcgc gttcgacgcc ttcccgatga cgcaccacgt
     4681 cgagtgcgtc gccctgctga cgaagagcgg ccccgcgtag tcgtgcccga ggacttcgtg
     4741 gttgcgcgca acaccgagcc cgggtcgacc ctgccgtacc tgctgcggat ccccctcggt
     4801 gacggggtca tgctcaaggc gaaggacacc tggccacgca ccggcaaggt ctactgccac
     4861 cggctcgatg agtggcccgc ggatgcggag atcatcgaga gggtcccggt gcgctcgtgc
     4921 gtgcgacgcg gggcggccat cgacctcgtg ctcgatcgcg gccgcgagaa ccggtcgcaa
     4981 ctggtcatca ccaccgcccg cggccggcag atgatcttct ggcagacctc ccgcacggcc
     5041 aagcaggccc gcccggccgt ggccgtgccc ggagctcgag ccagtggcct ggccgacgtg
     5101 gagatctccg tcgacatccg ggagcggtac ccgttcacgt tcgccgaccg tcaggtgacg
     5161 acccggcgcg aaccgttgag ggcaggggac tacgggctcg tcgtcgacgg ggtcttgcag
     5221 gccagtgtgg agcgtaagtc cttgactgac ctggtgtcgt cgctgacgac gggcacgttg
     5281 aagttccagc tcaccgaact gtcggcgatc cctcgcgcgg cggtggtcgt cgaggaccgg
     5341 tactcacggg tcttcgccct cgaacacgtg cgcccgagcg tcgtggccga cgggatcgcc
     5401 gagtgccaca tccggttccc gagcgtgccg atcgtcttct gcgagacccg caaactcgcc
     5461 caggaatgga cctaccgctt tctcgctgcg gcgcgcgtcg gcctcgccga ggaggtggtc
     5521 ggggacttgg cggttcgcgg gctggaggcg gcgccgcccc ttgccccgcc gccgccgaca
     5581 ccggcgcagg tgcggcgctg ggcagttgcc cacgacatcg ccgtctccga ccgtggccgg
     5641 atcgcggggt cggttctgga gcgctacctc gaggcccgtg ctcgtggaga gctctgatgc
     5701 ccggccgccg ggctcctgcc cgatctcgcg gccctcggcg ggccgcactt cgacgcgtcc
     5761 ggggtggatg ccggggtgcg cgacctctac gagcacacgg cgaagtggcg gatggatgcg
     5821 tggtcgcagt ggtcggcgcc gttcctgcca gacggtgccc tcatcaccgc cctgttcggc
     5881 actaccgcga cgctcacgcc gtgcgcgccg accaccacct gtggctgggc cgcgcgctcg
     5941 tcgtccgtct gcactaccgg atggaacggc tggcttgaga agtccggtgg ctctgggtct
     6001 cgccgggtgg cacaccgcgg cataggctcg acgcgaccga cccggtgtgg ttagatatct
     6061 tgacatcaag ataaatcacg ggaatcgcgc gacgacgcac gacctgaagg gagccccggt
     6121 ggccagcacg aacagcttcg acgcccacgg cacactcgag gtgggtgagc agtcctacga
     6181 gatctaccgc ctcgcagcgg tcgaggggag cgacacgctc ccgtacagcc tcaaggtgct
     6241 gctcgagaac ctcctgcgca ccgaggacgg cgccaacatc acggccgagc acatccgtgc
     6301 cctcgccggc tgggacgaga acgccgagcc cgacaccgag atccagttca cgccggcccg
     6361 cgtgatcatg caggacttca cgggtgtgcc ctgcgtcgtc gacctcgcga ccatgcgcga
     6421 ggccgttgcc gacctcggcg gcgacccgag caagatcaac ccgctcgccc cggccgagct
     6481 cgtcatcgac cactccgtgc agatcgacgt cttcggccgg tccgacgcgt tcgagcgcaa
     6541 cgtcgagttc gagtacgggc gcaacttcga gcgctaccag ttcctgcgct ggggccagac
     6601 ggcgttcgac gacttcaagg tcgt
//
LOCUS       DAOSOC010000137         6570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_11812_length_6570_cov_9.639447, whole genome shotgun sequence.
ACCESSION   DAOSOC010000137 DAOSOC010000000
VERSION     DAOSOC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11812_length_6570_cov_9.639447"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PL014_10040"
     CDS             complement(<1..898)
                     /locus_tag="PL014_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT57474.1"
                     /translation="MDQFAQQLVNGLTLGSLYALIAVGYTVVYGIVQLINFAHGEIFM
                     IGAFGALSTWLIFFEGETALWTLPIMLVGAIAASVLTAVTMERIAYRPLRNAPRLAPL
                     ITAIGISIFLQEAVRLGFNDPTLFLSNWNPLDFPSAKQKVPFPQIDVVTGQAFTLGGV
                     TIQRSALFTIGCLVMCAAGLWYFVNRTKTGRGMQAVSQDPDTARLMGINVDRIIMVAF
                     AVGATLAAVAGVAQGLQVTNINFKMGFLAGLKAFTAAVLGGIGNVWGAVVGGLTLGIV
                     EAMAVQFIPGTFGGSPWKDVWAF"
     gene            complement(1113..2339)
                     /locus_tag="PL014_10045"
     CDS             complement(1113..2339)
                     /locus_tag="PL014_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT57475.1"
                     /translation="MPQRSVVAVGASLLVASLALAGCGSRSDDTEGTDTGSAENKVAK
                     IGVIAPLSGDLAALGKGIQNSVDLAIRQANEAKTIPGWTLELAAEDDQATPDVGKNAA
                     TKLAADKDVVGVVGTLNSSVAQSVQPVLAAANIVQVSPANTNPSLTQGADFATAPKRT
                     YPTYFRTCTTDSVQGPFAARYLFETAGIKEVATVHDKKTYGQGLVEAFTAEYEKLGGK
                     IVAAETINPDDDKYDAVISKIKPSNPKTVYYGGEYPQAGPFSQQMKAAGLNVPLMGGD
                     GIYSGEFIKLAGATAEGDLATSVGAPTDTLESAKDFIAAYEAAGFAEPYEAYGAYAFD
                     AANAIIQALKVSLASADSAEAARQATVDAMSKVSFDGVTGAVAFDEFGDTTSRVLTVY
                     GVKGGKWEAVNTSEFK"
     gene            2609..3124
                     /locus_tag="PL014_10050"
     CDS             2609..3124
                     /locus_tag="PL014_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HOT57476.1"
                     /translation="MSTTRSSDHHDTSAPAGPHPDPSAARCGCTSRRESLLAAGVVVA
                     GAAGLAACGSGEAVGAAASSAASAAASAAASVAGDAIAAASVPVGGGVILDRLKVVVT
                     QPTAGDFKAFSAVCPHRGCTVNAVEGGQITCPCHGSAFDIATGAVTKGPATSGLPTKG
                     VTVGADGITVS"
     gene            3157..5124
                     /gene="uvrC"
                     /locus_tag="PL014_10055"
     CDS             3157..5124
                     /gene="uvrC"
                     /locus_tag="PL014_10055"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT57477.1"
                     /translation="MADPSTYRPRPGEIPTEPGVYRFRDRDGRVIYVGKAKSLRPRLS
                     SYFQDVSALHHRTATMVRTGASVEWTVVSTEVEALQLEYAWIKEFDPRFNVRYRDDKS
                     YPYLAVTMGEEFPRAQVLRGAKRKGTRYFGPYAHAWAIRETLDLALRVFPVRTCSSGV
                     FKRASQVGRPCLLGYIDKCSAPCVGRVDAQRHREIAEDFCDFMAGETGRFVTRLTREM
                     KDAAAELDFERAARLRDDIGALERVLEKSAVVLPDATDADVFALAEDELEVAVQLFHV
                     RGGRVRGQRGWVAERDAESTPEVVEHLLQRVYGSEAGEAVPREVLVPVLPDDAPALAD
                     WLSGLRGSRVDLRVPQRGDKRTLMETVARNATQSLARHKVARAGDLTTRSQALQQIQD
                     ALGLDDAPLRIECFDISHVQGTQVVGSMVVFEDGLPRKSEYRRFIVRGDETGATDDTA
                     AMHEVLTRRFRRGRREDDEAAAADGSQDDTDAGAARPARFAYRPNLVVVDGGLPQVNA
                     AARALADAGAQDVAVVGLAKRLEEVWIPGEGHPLVLARSSEGLYLLQRLRDEAHRFAV
                     TFHRQRRSKAMTASRLDGIPGLGEKRRKALLKAFGSVKRIAAASVDELCEVPGIGPAL
                     AAVIAAQLASADTMPAVNLATGEVVDEGNEP"
     gene            5160..6050
                     /gene="rapZ"
                     /locus_tag="PL014_10060"
     CDS             5160..6050
                     /gene="rapZ"
                     /locus_tag="PL014_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOT57478.1"
                     /translation="MPSGGVPEANDFLIVTGMSGAGRSTAANVIEDRGWFIVDNLPPQ
                     LLSSMAELAGRVRNDSGTDLQIAAVVDVRARAFHSELHAGLAALRDAGWRPHLIFLDA
                     TDEALVRRFESVRRPHPLQGEGRLLDGIQAEREMLRDLRAEAQVVIDTSGLNIHQLGR
                     KLTPLLGVESGPSLRLALMSFGFKYGVPLDADFVLDMRFLPNPFWVPELKALTGVDEP
                     VADWVMAQDGAQEFVDAVVQLMGPVTAGYLAEGRRYATIGIGCTGGKHRSVAMTQAIA
                     ARLTTEQVATFVVHRDLGRE"
     gene            6047..>6570
                     /locus_tag="PL014_10065"
     CDS             6047..>6570
                     /locus_tag="PL014_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HOT57479.1"
                     /translation="MTGPVVAALGGGHGLAATLTALRYVTDEITAIVTVADDGGSSGR
                     LRDEFGVLPPGDLRMALAALCAESEWGHTWRDVLQHRFAGDGPLGGHALGNLLIVALW
                     ELHDDPVAGLDLVGRLLQAQGRVLPMAAVPLAIEADVSGLDPADPTAVQVVLGQAQVA
                     VTPGTVERVRLVPAA"
BASE COUNT          985 a         2210 c         2363 g         1012 t
ORIGIN      
        1 cgaaggccca gacgtccttc cacgggctac caccgaaggt gcccgggatg aactggacgg
       61 ccatggcctc gacgatgccc agggtgagac caccgacgac ggcgccccac acgtttccga
      121 tgccgccgag gacggccgcg gtgaacgcct tcagaccggc gaggaagccc atcttgaagt
      181 tgatgttcgt gacttgcaga ccctgcgcga caccggcgac ggcggccagc gtggcaccga
      241 cggcgaaggc gaccatgatg atccggtcga cgttgatgcc catgagacga gcggtgtcgg
      301 ggtcctggga gacggcctgc atgccgcgcc ccgtcttcgt gcggttgacg aagtaccaca
      361 ggccggccgc gcacatcacg aggcaaccga tggtgaacag ggccgagcgc tggatcgtga
      421 caccgcccag cgtgaacgcc tggccggtga ccacgtcgat ctgcgggaag ggcaccttct
      481 gcttggcgct cgggaagtcg agggggttcc agttggagag gaacaacgtc gggtcgttga
      541 accctaggcg caccgcttcc tggaggaaga tcgagatacc gatcgcggtg atcagcggcg
      601 cgaggcgtgg cgcgttgcgc aggggacggt aggcgatgcg ctccatggtc acggcggtga
      661 gcaccgaggc ggcgatcgcg ccgaccagca tgatcggcag cgtccacagg gccgtctcgc
      721 cctcgaagaa gatcagccag gtcgacaggg caccgaaggc gccgatcatg aagatctcgc
      781 cgtgggcgaa gttgatcagc tggacgatgc cgtacacgac ggtgtagccg acggcgatga
      841 gggcatagag cgatcccagg gtcagcccgt tgacgagctg ctgggcgaat tggtccacga
      901 gggtctcctc ggtgttcgcg aacggatgtg tggacttctg agtgtggttt tggtcacctg
      961 tccgatcgac aggctggggg gagcgtagcg gctgtcggcg tcgctgtcag gcgccacgcg
     1021 caaccgctgt gggacacgaa cagcgggggt ggttcgggcg aaccaccccc gctgtcaggt
     1081 gtgcatcacg gcagggccgt cagtgctggg tcttacttga actccgaggt gttcacggct
     1141 tcccacttgc cgcccttgac accgtagacg gtgaggacgc gggaggtcgt gtcgccgaac
     1201 tcgtcgaagg ccaccgcacc ggtgacaccg tcgaaggaga ccttggacat ggcgtccacg
     1261 gtcgcctggc gagcggcctc ggccgagtcg gcgctggcca gcgagacctt gagggcctgg
     1321 atgatggcgt tggccgcgtc gaaggcgtac gcaccgtagg cctcgtacgg ctcggcgaac
     1381 ccggctgcct cgtaggcggc gatgaagtcc ttggccgact cgagggtgtc ggtcggggca
     1441 ccgaccgagg tggcgaggtc gccctcggcg gtcgcaccgg cgagcttgat gaactcaccg
     1501 gagtagatgc cgtcgccgcc catgagcggg acgttgaggc cggcggcctt catctgctgc
     1561 gagaacggac cggcctgcgg gtactcgcca ccgtagtaga cggtcttcgg gttggacggc
     1621 ttgatcttgc tgatgacggc gtcgtacttg tcgtcgtccg ggttgatcgt ctcggctgcg
     1681 acgatcttgc cgccgagctt ctcgtactcc gcggtgaagg cctcgacgag accctgacca
     1741 taggtcttct tgtcgtggac cgtcgcgacc tccttgatgc cggccgtctc gaacaggtag
     1801 cgcgccgcga aggggccctg gaccgagtcg gtcgtgcagg tacggaagta cgtcgggtag
     1861 gtgcgcttgg gggccgtcgc gaagtccgca ccctgggtca gggaggggtt cgtgttggcc
     1921 ggcgagacct gaacgatgtt ggcggcggcg agaaccggct ggacgctctg cgcgacgctg
     1981 gagttcagcg tgccgacgac gcccacgacg tccttgtcag cggcgagctt cgtcgcggcg
     2041 ttcttgccga cgtcgggggt ggcctggtcg tcctcggcag cgagctcgag ggtccagccc
     2101 gggatcgtct tggcctcgtt ggcctgacgg atcgcgaggt ccacggagtt ctggatgccc
     2161 ttgccgagtg cggcgaggtc gccggacagg ggagcgatga cgccgatctt ggcgaccttg
     2221 ttctctgcgg aaccggtgtc ggtcccttcg gtgtcgtcac tgcgcgagcc acaaccggca
     2281 agcgcgagcg aagccacgag cagcgaggcg ccaacggcga ccacggaacg ctggggcacg
     2341 tttcctcctg ggataactga gtgagatgag cgggtagctc ctgcgcgcgt ctggcgagca
     2401 agcgaactgt aaccacacgc gcacccctcg acccagcatt gcgtgcagtc tggtgccgac
     2461 ttgttatctg aggttgacca aacccgcaac cgctgcagtc acacgacggc gtcgttctca
     2521 caccgttctg tcaggttcca tggtgatact gcagcagccg tcgcgcccac tgcacgggcg
     2581 cgcttcgtcg acgagtcgga ggaccaccat gagcaccacg cgatcgagcg accaccacga
     2641 cacctcggcc ccggcgggtc cgcaccctga cccctcagcc gcgcggtgcg gctgcacgtc
     2701 gcgacgcgag agcctgctcg ccgcgggagt cgtcgtcgcc ggcgcggccg gcctcgccgc
     2761 gtgtggttcg ggcgaggccg tcggtgctgc cgcgtcctcc gcagcctccg ctgcggcgtc
     2821 ggcggcagcc tccgtcgcgg gcgacgccat cgctgccgcc tcggtccccg tcgggggcgg
     2881 tgtcatcctc gacaggctca aggtcgtcgt cacgcagccg acagccggtg acttcaaggc
     2941 gttctcggcc gtgtgcccgc accgggggtg cacggtgaac gccgtcgagg gcggacagat
     3001 cacctgcccg tgccacggca gtgccttcga catcgcgacc ggcgcggtca cgaagggccc
     3061 ggccacctcg ggcctgccga ccaagggcgt caccgtgggc gcggacggca tcaccgtctc
     3121 gtgaaggctg tcggcccgcc gacatagggt tggcgcgtgg ccgacccgtc gacgtaccgc
     3181 ccgcggcccg gcgagatccc caccgagccg ggggtctacc ggttccgcga tcgcgacggt
     3241 cgcgtcatct acgtcggcaa ggccaagagc ctgcgcccgc ggttgtcgtc ctacttccag
     3301 gacgtctccg ctctgcacca ccgcacggcc accatggtgc gcaccggcgc cagcgtggag
     3361 tggacggtgg tctccaccga ggtcgaagcc ctccagctcg agtacgcgtg gatcaaggag
     3421 ttcgacccgc ggttcaacgt ccgctaccgg gacgacaagt cataccccta cctggcggtg
     3481 accatggggg aggagttccc ccgcgcccag gtgctgcggg gggccaagcg caagggcacc
     3541 cggtacttcg gcccgtacgc ccacgcgtgg gccatccgcg agaccctcga tctcgccctg
     3601 cgcgtgtttc ccgtgcgcac gtgctcgtcc ggggtgttca agcgggccag tcaggtgggt
     3661 cgtccctgcc tgctcgggta catcgacaag tgcagcgcac cgtgtgtggg gcgggtcgac
     3721 gcccagcgcc accgtgagat cgccgaggac ttctgcgact tcatggcagg cgagaccggc
     3781 cgcttcgtca ctcgcctcac ccgcgagatg aaggatgccg cggccgagct ggacttcgag
     3841 cgcgccgccc gcctgcgcga cgacatcggc gcgctggagc gggtcctgga gaagtcggcc
     3901 gtggtgttgc ccgacgcgac ggatgccgac gtgttcgccc tcgccgagga cgagttggag
     3961 gttgccgtgc agctcttcca cgtgcgggga gggcgcgtgc gcgggcagcg gggctgggtc
     4021 gccgagcgcg atgccgagtc gacccccgag gtcgtcgagc acctgcttca gcgggtctac
     4081 ggctcggagg ccggtgaggc cgttccgcgt gaggtgctcg ttcccgtcct cccggacgac
     4141 gcgccggccc tggccgactg gctgtcgggc ttgcggggaa gccgggtcga cctgcgcgtt
     4201 ccgcagcgtg gtgacaagcg cacgctcatg gagacggtgg cccgcaacgc gacgcagagc
     4261 ctggcgcgac acaaggtggc cagggctggt gacctcacca cccgcagtca ggcactccag
     4321 cagatccagg acgcactggg gttggacgac gcacccctgc gcatcgagtg cttcgacatc
     4381 agccacgtgc agggcaccca ggttgtcggc tccatggtcg tcttcgagga cgggctgccc
     4441 cgtaagtccg agtaccgccg cttcatcgtg cgcggcgacg agaccggggc caccgacgac
     4501 accgcagcga tgcacgaggt gctcacccgc cggttccggc gcgggcgtcg ggaggacgac
     4561 gaggccgccg cggctgacgg cagccaggac gacacggacg cgggagcggc ccgcccggcc
     4621 agattcgcgt accgaccgaa cctcgtcgtc gtcgacggcg gcctgcccca ggtcaacgcc
     4681 gccgcccgcg ccctcgccga cgcgggtgcc caggacgtcg ccgtggtggg gctcgccaag
     4741 cgcctcgagg aggtctggat cccgggggag ggccaccccc tggtgctcgc gcggtccagt
     4801 gagggcctct acctgctgca gcggctgcgt gacgaggcac accgcttcgc cgtgaccttc
     4861 caccgccaac gccggtccaa agccatgacc gcgagccggc tcgacggcat cccgggtctg
     4921 ggcgagaagc ggcgcaaggc cctgctcaag gcgttcggtt cggtcaagcg gatcgccgcg
     4981 gcctcggtcg acgagctctg cgaggttccc ggaatcgggc ctgcgctggc agccgtgatt
     5041 gcggcacagt tggcctccgc ggacaccatg cccgcagtga atctcgcgac cggagaggtc
     5101 gtcgacgaag ggaacgagcc gtgaccccgg accacgagca gcctgacggc gcatcagtcc
     5161 tgccctcggg aggtgtcccc gaggccaacg acttcctcat cgtcacgggg atgagtgggg
     5221 ccgggcgctc gacggcggcc aacgtcatcg aggaccgcgg ctggttcatc gtcgacaacc
     5281 tgccaccgca gctgctgtcg tcgatggccg agctcgcggg ccgggtacgc aatgacagtg
     5341 gcaccgacct gcagatcgcc gcggtcgtcg acgtccgggc cagggccttc cactccgagc
     5401 tgcacgcggg gctcgcggcg ttgcgggatg ccggttggcg gcctcacctg atcttcctcg
     5461 acgccaccga cgaggcgctc gtgcgccgat tcgagagcgt gcgtcgcccg cacccgctcc
     5521 agggagaggg tcggctgctc gacggcatcc aggccgagcg cgagatgctg cgcgacctgc
     5581 gcgccgaggc ccaggtcgtc atcgacacct cgggcctgaa catccaccag ctcggccgca
     5641 agctcacgcc cctcctgggg gtcgagagcg gcccgagcct gcgactggcc ctgatgtcct
     5701 tcggcttcaa gtacggcgtc ccgctcgacg ccgacttcgt gttggacatg cggttcctgc
     5761 ccaacccctt ctgggtgccc gaactgaagg ccttgaccgg agtcgacgaa ccggtcgccg
     5821 actgggtcat ggcccaggac ggcgcgcagg agttcgtgga tgccgtggtg cagctgatgg
     5881 gcccggtcac cgccgggtac ctcgccgagg gtcgtcgcta cgcgacgatc gggatcggct
     5941 gcaccggagg caaacaccgc tccgtggcga tgacccaggc gatcgcggca cgactcacga
     6001 cggagcaggt ggcgaccttc gtcgtccacc gtgacctggg gcgcgagtga ccggccccgt
     6061 cgtcgcggcc ctcggcggag ggcacggact ggccgcgacc ctgacggcgc tgcgatacgt
     6121 gaccgacgag atcaccgcca tcgtcaccgt cgcggacgac ggcggctcga gtgggcgact
     6181 gcgtgacgag ttcggggtgc tccccccggg agacctgcgg atggcactgg ccgccctgtg
     6241 tgccgagagc gagtgggggc acacgtggcg ggacgtcctg cagcaccggt tcgcgggcga
     6301 cgggccgctc ggcgggcacg ctctgggcaa ccttctcatc gtcgcgctct gggagctgca
     6361 cgacgacccg gtcgccgggc tcgacctcgt cggccggctc ctccaggccc aggggcgggt
     6421 gctgccgatg gcggcggtcc cactggcgat cgaggcagac gtgagcgggc tggacccggc
     6481 tgacccgacg gccgtgcagg tggtgctcgg ccaggcccag gtggcggtca ccccgggcac
     6541 ggtcgagcgg gtacggctcg tgccggcggc
//
LOCUS       DAOSOC010000138         6569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_11813_length_6569_cov_12.008443, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000138 DAOSOC010000000
VERSION     DAOSOC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11813_length_6569_cov_12.008443"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PL014_10070"
     CDS             <1..392
                     /locus_tag="PL014_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57480.1"
                     /translation="TFKSYYKIPRHQGSTENCVAHNGSIIPVNGRDVMIQSWYQGGVS
                     LWEFTDSANPKELDYFERGAINADSLVLGGTWSAYYYNGFVYSSDITKGLDVLMIKDP
                     TLRKANSVRLGEFNAQTQPVYPIKPGK"
     gene            complement(480..3098)
                     /locus_tag="PL014_10075"
     CDS             complement(480..3098)
                     /locus_tag="PL014_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57481.1"
                     /translation="MHGTLKDCAVGTTRHRPRFGGLGILVLTAALFGTPALADDGLST
                     GATSRYVIDAKAAVLKVDVTLELRNTTPDQGNRYFYYNFFTVPVPAGAENLRASSGGS
                     TLKVRLSDSEDPSTALARISFPDLRHGRSRTIRLTYTVPGEKPRAADSTRVGPGYATF
                     SVNGFGDPGRNTVQVVAPTSMTFDATSEDFTASDKGATTTHTVSSSDPDGFWAVVSLR
                     DPANSRKATVDVPGTSLELHGYQDDARWTEFVAEQVTAGLPELERLTGTSWPGGLERI
                     REDASPAIQGYDGWFDPGDDEIVIGEDLDADLIFHELSHAWVSSERFSERWVYEGLAQ
                     VLAERTVTATGGKVVPHPKASRKDRDAVALNEWGGSPSVPFDDVDSYAYPAAYAATKA
                     LVADLDDAQLAAVVSAGIRGESAYDPAGTRDSAGVSTSWTRWLDLLETRGGVKDAAGV
                     FRTWALTADQRAQLAPRATARAAYAAVDEGDGDWLPPEGLRAAMTRWDFDRATTVTEQ
                     VVELGPAVTQVQDAAAKGDLPVPEAVRETYEKAEDDEQYAALASSLPRAARVISAVGA
                     AVDDAATDRDPVSALGAAVLGVQDSAAEAVALLEAGKLDAAAAAAETASGRADKSLVV
                     GLAGPLLVALGLVGGVIVTRRVLRGRSTAGIPVDGEPLDATLGSMSATPASSPAQPGN
                     LSGHRTPEELEAFLPVLLAAPRDVGTLDLVVRRPAEGVREVLDEGELDLVVGLVGDTW
                     AERGSSRSADGGPHPDMQLNVMSSRMVGFLAGDPDRRALAGDQLYLDLDLSHENLPTG
                     SRLTIGDPAERGAVIEVTEQPHTGCAKFIDRFGAEAMRFVNGSVGRPLRLRGLNARVV
                     VPGRVRPGDAVTVTRA"
     gene            complement(3151..>3360)
                     /locus_tag="PL014_10080"
     CDS             complement(3151..>3360)
                     /locus_tag="PL014_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOT57482.1"
                     /translation="ARRGQAWHGTDRQVRGAVLQLLRDAPRALSRTALADAGPDAGQL
                     DRCIASLVEDGLVEPVADAHFRLPH"
     assembly_gap    3361..3460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3461..3857)
                     /locus_tag="PL014_10085"
     CDS             complement(<3461..3857)
                     /locus_tag="PL014_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOT57483.1"
                     /translation="MREVADLHENILRWYSRHARALPWRDAATTPWGVFVSEVMAQQT
                     PVARVEPVWLSWMQRWPTPAHLAAESPGEVVRAWGRLGYPRRALRLREAAVAIVECHD
                     GQVPSDEETLRTLPGVGAYTAAAVAAFAFG"
     gene            complement(3905..4987)
                     /gene="disA"
                     /locus_tag="PL014_10090"
     CDS             complement(3905..4987)
                     /gene="disA"
                     /locus_tag="PL014_10090"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HOT57484.1"
                     /translation="MPTQLPDEELLRATLAAVAPGTELRDGLERILRGRTGALIVLGN
                     DKVVESLATGGFPLDIEFSATRLRELAKMDGGIVCDRDVTRIVRAATQLVPDPSIETS
                     ESGTRHRTAERVAKQTGFPVVSVSQSMRIIALYVAGRRVVLEDSSAILSRANQALQTL
                     ERYKSRLDEVTGTLSALEIEDLVTVRDVANVLQRLEMVRRIKDEIAEYVVQLGTDGRL
                     LSLQLEELTGGLSDDLGLVIRDYLPETRASLTLDDAIANLEAIDSTELLDPSAGARAI
                     GFTIVGDSLDSSVSPLGHRLLSKIPRLPGAIVDRLVAHFGSLQKLLAANVDDLMDVDG
                     VGEGRARLVREGLSRLAESSILERYV"
     gene            complement(5130..6536)
                     /gene="radA"
                     /locus_tag="PL014_10095"
     CDS             complement(5130..6536)
                     /gene="radA"
                     /locus_tag="PL014_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOT57485.1"
                     /translation="MSGTASRRSARSAAAYRCSECGWQAVKWVGRCGECQAWGSVTEV
                     GAVTARTTAASAVDRPAIPIGEVDVRRAVARPTGVPEFDRVLGGGIVPGAVVLVAGEP
                     GIGKSTLLLDVAARAARSGDHGDRKVLYVSGEESSAQVRTRAERIEAMARSLYLASET
                     DLATVLGQIDALQPELVIVDSVQTISSAEVEGSAGNVSQVREVAASLIQVAKARGIAV
                     LLVGHVTKDGSIAGPRVLEHLVDVVVQFEGDRHSRLRLVRAVKNRFGPTDEVGCFDLS
                     DVGIVGLADPSALFLSGRTAAVAGTCVTVTLEGRRPLVTEVQALVTASTLSTPRRATS
                     GLDTARVNMILAVLDKRAAAPIGGSDAYLSTVGGVRLTEPAADLAITLALAGAITDRP
                     LPHGTIAFGEVGLAGEIRPVTGAHRRLAEAARLGFTRAVVPPGVAGAGPVPPGLHIVE
                     SATVSEAVGRGLYDGVTR"
BASE COUNT          965 a         2293 c         2303 g          908 t
ORIGIN      
        1 tgaccttcaa gtcgtactac aagatccccc gtcaccaggg cagcaccgag aactgcgtcg
       61 cccacaacgg atcgatcatc ccggtcaacg gccgtgacgt catgatccag tcctggtacc
      121 agggcggtgt ctccctgtgg gagttcaccg actccgccaa cccgaaggag ctcgactact
      181 tcgagcgcgg agcgatcaac gccgacagcc tcgtgctcgg tggcacctgg tccgcgtact
      241 actacaacgg gttcgtctac agctcggaca tcaccaaggg cctcgacgtc ctgatgatca
      301 aggacccgac gctgcgcaag gccaactcgg ttcgcctggg cgagttcaac gcccagaccc
      361 agccggtcta ccccatcaag cccggcaagt gagcctcgcc tgagggaacc agcacacgaa
      421 cggcccaccc cgcacggggt gggccgttcg ctgttctccg actcgacgtg ctccggtgcc
      481 tacgcgcggg tcacggtgac cgcgtcgccg gggcgcaccc gtccggggac gacgacccgg
      541 gcgttgaggc cacgcaggcg tagtggccga cccaccgacc cgttgacgaa ccgcatcgcc
      601 tcggcgccga agcggtcgat gaacttggca caacccgtgt gcggctgctc ggtcacctcg
      661 atgacggcgc cgcgctccgc cgggtccccg atcgtcagcc gcgacccggt cggtaggttc
      721 tcgtgcgaca ggtcgaggtc gaggtagagc tgatccccgg cgagcgcccg acggtcggga
      781 tcccccgcga ggaaacccac catgcgtgag ctcatgacgt tgagctgcat gtccgggtgc
      841 gggccgccgt cggcactgcg actgctgccc cgctcggccc acgtgtcgcc gaccagaccg
      901 acgacgaggt cgagctcgcc ctcgtcgagc acctcgcgca ctccctcggc cggccgtcgc
      961 acgacgaggt ctagggtgcc gacatcgcgt ggggcggcga gcagcacggg caggaacgcc
     1021 tccagctcct ccggcgtgcg gtgacccgag aggttcccgg gctgggctgg ggaggatgcc
     1081 ggggtcgcac tcatggagcc cagtgtggcg tccaggggtt caccgtccac cgggattcca
     1141 gcggtcgacc ggccgcggag tactcgccgg gtcacgatca ccccaccgac gaggccgagc
     1201 gcgaccagca ggggcccggc gagtccgacg accagggact tgtcggcacg ccccgaggcg
     1261 gtctcggcgg cggccgctgc cgcgtcgagc ttgccggcct cgagcagcgc gaccgcttcg
     1321 gcagcgctgt cctgcactcc cagcacggcc gccccgaggg cgctcacggg gtcgcggtcc
     1381 gtggcggcgt cgtccacggc ggcccccacg gcgctgatca cccgtgccgc ccggggcaat
     1441 gatgacgcca gggccgcgta ctgctcgtcg tcctcggcct tctcgtacgt ctcacgcacg
     1501 gcctcgggaa cgggcagatc gcccttggcc gcggcatcct gcacctgcgt gaccgcgggg
     1561 cccagctcga ccacctgctc cgtcaccgtg gtggcgcgat cgaagtccca ccgcgtcatg
     1621 gcggcacgca ggccctccgg tgggagccag tcaccgtcgc cctcgtcgac ggcggcgtac
     1681 gctgctcgcg ccgtcgccct gggagcgagc tgagcccgtt ggtcggcggt gagggcccag
     1741 gtccggaaga cgccggcggc atccttcaca ccacctcggg tctccaggag gtccagccac
     1801 cgtgtccagg acgtcgacac cccggccgag tctcgggtgc cggcagggtc gtaggcgctc
     1861 tcgccccgga tgccggcact cacgaccgcg gccagctggg catcgtcgag gtcggcgacc
     1921 agggccttgg tggcggcgta ggccgcgggg taggcgtagg agtcgacgtc gtcgaaggga
     1981 acgcttggcg agccacccca ctcgttgagc gccaccgcgt cgcggtcctt gcgggaagcc
     2041 ttcggatggg ggaccacctt tcctcccgtc gccgtgacgg tgcgttctgc gagcacctgg
     2101 gccaggcctt cgtagaccca ccgctcgctg aaccgctcgc tcgacaccca cgcgtgggac
     2161 agctcgtgga agatcaggtc ggcgtcgagg tcctcgccga tgacgatctc gtcgtcgccg
     2221 gggtcgaacc agccgtcgta accctggatg gccggggacg catcctcccg gatccgctcg
     2281 aggccaccgg gccacgacgt cccggtgagc cgttccagct ccggcagacc ggcggtgacc
     2341 tgctctgcca cgaactccgt ccaccgggcg tcgtcctggt aaccgtggag ctccagcgag
     2401 gttccgggca cgtccacggt tgccttcctg ctgttcgccg ggtcgcgcag ggacaccacc
     2461 gcccagaacc cgtcgggatc ggatgaggag acggtgtggg tcgtcgtggc gcccttgtcg
     2521 gacgcggtga agtcctcact cgtcgcatcg aaggtcatcg acgtcggggc caccacctgc
     2581 acggtgtttc gcccggggtc cccgaacccg ttgacgctga aggtggcata tcccgggccg
     2641 acccgcgtgc tgtcggcggc acggggcttc tcacccggca ccgtgtaggt gagtcggatg
     2701 gtgcgggagc ggccgtgacg caggtccggg aaggagatcc tggccagggc tgtcgacgga
     2761 tcctcgctgt cgctgagccg aaccttcagc gtggacccac ccgagctggc ccgcaggttc
     2821 tcggcgccgg ccgggaccgg gaccgtgaag aagttgtagt agaagtacct gttcccctgg
     2881 tcgggtgtgg tgttgcgcag ctcgagggtg acgtcgacct tcagcacggc ggccttggcg
     2941 tcgatgacgt agcgactggt cgcccccgtc gagagcccgt cgtcggccag tgccggcgtg
     3001 ccgaacagcg ccgcggtgag gaccaggatc cccagaccgc cgaacctcgg tcgatgtctc
     3061 gtcgtcccca ccgcgcagtc cttcagggtg ccgtgcaccc caccccgcgt gatgtgcctc
     3121 gggagcagcg taggccgact ggttgccgga tcagtgcggc agccggaagt gcgcgtctgc
     3181 gaccggctcg acgaggccgt cctcgacgag ggaagcgata caacgatcga gctgcccggc
     3241 atccggtccc gcgtcggcga gcgccgtgcg cgagagcgcg cgaggggcgt ctcgcagcag
     3301 ttggagcacg gcaccgcgta cctgccgatc ggtgccgtgc caggcctgac cgcgccgggc
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggccgaaggc aaatgcggca
     3481 actgctgccg ccgtgtacgc cccgaccccg ggaagggtgc gcaacgtctc ctcgtcggag
     3541 ggcacctgcc cgtcgtggca ctcgacgatc gccacggccg cctcgcgcag ccgcagcgcc
     3601 cggcgcgggt acccgagacg gccccaggcc cgcaccacct cgcccggtga ctcggccgcc
     3661 aggtgcgcgg gggtcggcca gcgctgcatc cagctcagcc agacgggttc cacgcgggcg
     3721 accggcgtct gctgagccat cacctcggag acgaagaccc cccacggggt ggtcgcagcg
     3781 tcacgccacg gcagcgcccg ggcatggcgc gagtaccagc gcaggatgtt ctcgtgcagg
     3841 tcggcgacct cgcgcaggtc gaccagggca cgtgacgcag gcactgggga cggagctgac
     3901 gcgctcagac gtagcgctcg aggatgctcg actcggccag gcgcgagagc ccctcgcgca
     3961 cgaggcgagc ccggccctcg ccgacaccgt cgacgtccat caggtcgtcg acgttggccg
     4021 ccagcagctt ctggaggctg ccgaagtgcg cgaccagacg gtcgacgatg gcacccggca
     4081 gacgcggaat cttcgagagc agacggtgcc cgagggggct gaccgaggag tcgagggagt
     4141 caccgacgat ggtgaacccg atcgcgcgag cgccagccga cgggtcgagc agttcggtgg
     4201 agtcgatggc ctcgaggttg gctatcgcgt cgtcgagggt caggctggcg cgtgtctcgg
     4261 ggaggtagtc acggatgacg agcccgaggt cgtcggacag acccccggtc aactcctcga
     4321 gctggaggct gagcaggcgc ccgtcggtgc cgagctggac gacgtactcg gcgatctcgt
     4381 ccttgatgcg gcgcaccatc tccaggcgct ggagcacgtt ggcgacgtcg cgcacggtga
     4441 cgaggtcctc gatctcgagg gccgacagcg tgccggtgac ctcgtcgagg cgggacttgt
     4501 agcgctcgag ggtctgcagg gcctggttgg cgcgcgacag gatggcgctg gagtcctcga
     4561 ggacgacccg acgaccggcg acgtacagcg cgatgatgcg catcgactgg ctgacggaga
     4621 cgaccgggaa gccggtctgc ttcgccaccc gctcggcggt gcggtgccgg gtgccggact
     4681 cgctggtctc gatgctggga tcgggcacga gctgggtcgc cgctcgcacg atccgcgtca
     4741 cgtcgcggtc gcagacgatg ccaccgtcca tcttggccag ctcgcgcagc cgcgtcgccg
     4801 agaactcgat gtcgagcggg aagccaccgg tggcgaggga ctcgacgacc ttgtcgttgc
     4861 cgaggacgat gagggcaccc gtgcgaccac gaagaatgcg ttcgaggccg tcacgcaact
     4921 cggtaccggg ggccaccgcc gcgagggtgg cgcgcagcaa ctcctcgtcg ggcagttgcg
     4981 tcggcactgc gagtcctccg gatccgtgtc cccaggccgt cgctgtctgc ggccgctggg
     5041 cgcatcctat ccggcacggg cctcctgccg cgcctgatca ccagcggcgg accgctcagg
     5101 accgcgtggc ggtcgtgagt cccacgcagt cagcgggtga caccgtcgta gaggccgcga
     5161 ccgaccgcct cgctgaccgt tgccgattcg acgatgtgca gtccgggagg aacgggcccg
     5221 gcgcccgcca cccccggggg gacgaccgcc cgagtgaagc ccagacgggc cgcctcggcc
     5281 aggcgacggt gcgctccggt cacgggccgg atctcgccgg ccagcccgac ctcaccgaag
     5341 gcgatggtgc cgtgcggcag cggcctgtcg gtgatggcgc ccgccagggc gagggtgatg
     5401 gccaggtcgg ccgcgggctc ggtgaggcgg accccgccca cggtcgacag gtaggcgtcg
     5461 ctaccgccga tgggcgcggc tgcccgcttg tcgagcacgg ccaggatcat gttgacccgg
     5521 gccgtgtcca gaccgctcgt cgcgcgccgc ggggtggaca gggtcgacgc ggtgaccagc
     5581 gcctgcacct cggtcacgag cggccggcgc ccctccaggg tcacggtcac acacgttccc
     5641 gccaccgcgg cggtacggcc agagaggaag agggcactcg ggtcggccag accgacgatt
     5701 ccgacgtcgg agaggtcgaa gcagccgact tcgtcggtcg ggccgaagcg gttcttcacg
     5761 gcacgcacga gccgcagcct gctgtggcga tcgccctcga actgcacgac gacgtcgacg
     5821 aggtgctcga gcacccgggg cccggcgatg gagccgtcct tggtgacgtg cccgacgagc
     5881 aggacggcga tgccgcgggc cttcgcgacc tggatgagcg aggcggcaac ctcacgaacc
     5941 tgcgagacgt tgccggcgga cccctccacc tccgcgctgc tgatcgtctg gacggagtcg
     6001 acgatgacga gctcgggctg cagggcgtcg atctggccga gcacggtggc gaggtcggtc
     6061 tcgctcgcca ggtagaggga tcgggccatg gcctcgatgc gctccgcgcg agttcgcacc
     6121 tgggccgagg actcctcacc gctgacgtag aggaccttgc ggtcgccgtg gtcacccgag
     6181 cgggccgccc gagcggcgac gtcgaggagc agcgtggact ttccgatgcc gggctcgccc
     6241 gcgacgagga cgaccgcacc gggcacgatg cccccaccga ggacgcggtc gaactcgggg
     6301 acaccggtcg gtcgagcgac ggcccgccgc acgtcgacct cgccgatcgg gatggccggg
     6361 cggtcgaccg ccgaggcggc cgtcgtgcgc gccgtgaccg cccccacctc ggtgacactg
     6421 ccccaggcct ggcactcgcc gcaccgaccg acccacttca ccgcctgcca gccgcactcc
     6481 gaacaacggt aggcagcggc ggagcgagcg gagcgacggg atgcggtgcc ggacatgcac
     6541 tccaccgtag gcgtcacccc cgacacacc
//
LOCUS       DAOSOC010000139         6474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12029_length_6474_cov_7.450693, whole genome shotgun sequence.
ACCESSION   DAOSOC010000139 DAOSOC010000000
VERSION     DAOSOC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12029_length_6474_cov_7.450693"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PL014_10100"
     CDS             <1..407
                     /locus_tag="PL014_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOT57486.1"
                     /translation="AVVLFLVIFFWTPPHYWPLSMAFRDDYSTAGVPMLPVERGPVAV
                     GRQIVAYSWVMVATSLVLVPVAPMGWVYVVTAVLSGALFIAEAHRLLRAARGGATGTA
                     LKPMRLFHYSITYVTLVFLAVAVDPLLYFPLS"
     gene            complement(902..1144)
                     /locus_tag="PL014_10105"
     CDS             complement(902..1144)
                     /locus_tag="PL014_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HOT57487.1"
                     /translation="MNVHPSALKHGMGAEDAAHAAHWAQWVEPLEDDDWPHRELRLGF
                     DTRARLLETIVLVFENGNELVIHAMPARKHYLDLLP"
     gene            complement(1141..1386)
                     /locus_tag="PL014_10110"
     CDS             complement(1141..1386)
                     /locus_tag="PL014_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOT57488.1"
                     /translation="MREDEVTDEQIEKWVDEAEAGYDVEALKRRGRGRPGRGAEPMQV
                     VAVRLTADEIAALDAVAEREHLTRSEAIRRALAHYAA"
     assembly_gap    1660..1759
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1760..2208)
                     /locus_tag="PL014_10115"
     CDS             complement(<1760..2208)
                     /locus_tag="PL014_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOT57489.1"
                     /translation="MTSTSTSSMTGSPRSDQALRPGSRWLSPILLANLVAEVAIVVTG
                     GLVRVTGSGLGCPTWPECVDGSITPTIEQAEGIHKWIEFGNRTLTGVLGLLALATIVA
                     VWRWAPRRAMKVAAVTVFVGVLVQAVLGGITVLLGLHPATVAAHFLVS"
     gene            complement(2257..3015)
                     /locus_tag="PL014_10120"
     CDS             complement(2257..3015)
                     /locus_tag="PL014_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT57490.1"
                     /translation="MSAALTPGAAPARARILSRAAFETRMLLANGEQLLVSLILPSLA
                     LVGLTLTSVPDLGPGARADLAFAGALALCVVSTAFTGQAIGTGFDRRAGVLRLLGTTP
                     LGRSGLLAAKALAVLSVLGVQVVVIGAIGVALGARAQPAGLVPAALTLLLGSACFVAL
                     ALLLAGTLRAEAVLAVANLVWVLLLGLGLLAPTESLPGILAPVAAALPSGALGDAMRL
                     ALVDGGWPLAQWAVLVVWGVGAAVLASRTFRWSD"
     gene            complement(3012..3722)
                     /locus_tag="PL014_10125"
     CDS             complement(3012..3722)
                     /locus_tag="PL014_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57491.1"
                     /translation="MATAVSVRDLRKSFGGVRAVDGATWGARAGQITAVLGPNGAGKT
                     TTIECLEGLQRPDAGTVQVLGVDPWGAGPQHRARVGVMLQEGGLPTTSRPVPLLRHLA
                     ALYAAPVDPDELVTRLGIDAFARTTVRRMSGGQRQRVALAAALLPRPDVLFLDEPTAG
                     LDPHARLDVWDLVRSEAARGACVVVTTHSFEEAERLADHVVILAAGTVVADGPLDQVR
                     AGRTLEAVYFELTAQATR"
     gene            3748..4422
                     /locus_tag="PL014_10130"
     CDS             3748..4422
                     /locus_tag="PL014_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOT57492.1"
                     /translation="MTRIDREAAPATAIAALPSVVALLAGRRGLAAGLAVLPAAIAAF
                     FRDPDRTPDHRPAPIDDVLSPADGKVMYVGPGQDLAAPEGEWQQISIFLSAFDVHVNR
                     APYGGRVTAVDFRLGKWLAAYKHESAHLNERSDITVEREVGGQVRRVHFRQIVGLMAR
                     RVVTRVSVGDEIATGQRIGLMKFGSRMDVFVPPECDLLVTAGDRVIAGETVIARWPAT
                     VTSVTS"
     gene            4419..5360
                     /gene="pssA"
                     /locus_tag="PL014_10135"
     CDS             4419..5360
                     /gene="pssA"
                     /locus_tag="PL014_10135"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOT57493.1"
                     /translation="MSTPAGGRRRILDRWRLVSPSGAQVVSLRQLPKRERARLMAPSM
                     FTTLNMVSGLSSVLLAFRGHFTIAAALIGVSIVLDIADGYVARLIGATSPFGLQLDSL
                     ADLISFGLAPAILVHTWALDEWPILAWLVAFLWLACAAFRLARFNVTIDPAADKRYFV
                     GLPSPGAAAVVMATIFALDSPDWGPGSGPWRAWFPLVVSVVPALLMVMTVRFRSFRNL
                     LAPQTRGARFTTAAVVVAILVGLALAPGLTAVVVAYGYVLTAPLGVLSAPLRERFLGA
                     ESIAPPRTRMPSVFLPLEEDDEVDGAQAAAVDGLDEG"
     gene            5408..6112
                     /locus_tag="PL014_10140"
     CDS             5408..6112
                     /locus_tag="PL014_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT57494.1"
                     /translation="MVNAETQVIESRTRDRVLRVVSQEGPVSIAALVARLGLTETALR
                     RHVDALHGEGLLEAREASGPKRRGRPAREWVLTDQGHRALVSDYDHLAGEALRFLGAT
                     AGPDAVTAFAEHRAADLENRYAERIATAGDDSASRATALVEALNADGFAASARPVGAA
                     DGPLTGVQLCQGHCPVQHVAAEFPQFCEAETEAFSRLLGVHVQRLATLAHGDHVCTTF
                     VPTPTTSTPTSERASS"
     gene            6109..>6474
                     /locus_tag="PL014_10145"
     CDS             6109..>6474
                     /locus_tag="PL014_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOT57495.1"
                     /translation="MTSHIEELNPGLKGLGTYEYGWADADTAGETARRGLNEEVVADI
                     SARKSEPQWMLDLRMKSLRLFGKKPMPSWGSDLTGIDFQNIKYFVKSTEKQATSWEDL
                     PEDIKNTYDRLGIPEAEKQR"
BASE COUNT          930 a         2281 c         2215 g          948 t
ORIGIN      
        1 ccgcagtggt gctcttcctc gtgatcttct tctggacccc gccgcactac tggccactgt
       61 cgatggcctt tcgcgacgac tactccacgg ccggggtccc gatgctgccg gtggagcgcg
      121 gcccggtggc cgtcggccgt cagatcgtcg cctattcgtg ggtcatggtc gcgacctcgc
      181 tcgtcctcgt tcccgtcgct cccatgggct gggtctacgt ggtgacggcc gtgctctccg
      241 gcgcgctgtt catcgccgag gcccaccgac tgctgcgcgc tgcccggggc ggcgccaccg
      301 ggaccgccct gaagccgatg cggctgttcc actactcgat cacgtacgtg accctggtct
      361 tcctcgcggt cgcggtcgac ccgctgctgt acttcccgct gtcctgagcc gaccccggcg
      421 gacagcggca tttcggatgc cgggagcggt gcgacgcgtc gtggcgacta ccgaacccgg
      481 atgcgagttg ccatcagcag catccgtccg acatcaccgg tgcacaggaa gcgcaccacg
      541 tgcggcatga gcggacttta tgaagatgct cgtccgtcgg tgaccttgtg gaaccacgcg
      601 gcagccatct gcgcagcccc tgcagccgac gacatctcgg caaccaggcc tccggccggc
      661 tcaaggctca tcccagtcgc tcggccccga acgccgtcat ccaaagggtt ccctcatcgt
      721 cagtgtgata cgtcctgagc ttgcgggacg gatgcactca agacctggaa gaaggccgac
      781 tcactcggtg ttggtggggc atcacgcacg aattcgagtc tctcaggtgc ggtgcgtact
      841 cgaccgccga tcggcatgga cgctcacact gctcacggca gctgcgtgtg ttctgctccg
      901 ctcagggcag aaggtcgagg tagtgcttgc gtgctggcat ggcgtggatc acgagctcgt
      961 tgccgttttc gaacacgagc acgatcgtct cgaggagacg cgcacgggtg tcgaagccga
     1021 gccgcagttc gcggtgtggc cagtcatcgt cttcgagcgg ctccacccat tgcgcccagt
     1081 gggctgcgtg ggcggcgtcc tcggcgccca tgccatgctt gagggcgctg gggtggacgt
     1141 tcacgcggcg taatgggcca acgcacggcg gatggcttcg gatcgggtga ggtgctcacg
     1201 ttcggcgact gcgtccaggg ccgcgatctc gtcggcagtg aggcggacgg cgacgacctg
     1261 catgggctcg gcgcccctgc cgggccgacc tcggccgcgg cgcttgagtg cctcgacgtc
     1321 gtagccagcc tcggcctcgt cgacccactt ctcgatctgc tcgtcggtga cctcgtcctc
     1381 acgcatgcga ttatcgtaaa cgaaaacatg cggttgtgtc gccaggaacg agtcaggtgc
     1441 cggaggccgt cgcggtccac ctccacggaa aggaccactg tccgcgtgag gtgatgcagg
     1501 gggcacgata ggtcgcgcgt gatgcgtcac ctgggtcggg cctggcgggt cgaggggtcg
     1561 agacatgccc gggatcgtcg actccgccgg gcgtgtctcg accggtcgat tggccagggc
     1621 caaacgtccg acattcgtca cgagcgcgcg caacacatcn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnng agacgaggaa gtgggcggca accgttgccg ggtgcagccc
     1801 gagcagcacc gtgatcccgc cgagcacggc ctggacgagg acaccgacga atacggtgac
     1861 agcggccacc ttcatggccc gccgcggtgc ccagcgccac acggcgacga tggtcgcgag
     1921 ggcgagcagc ccgagcaccc cggtcagggt gcggttgccg aactcgatcc acttgtggat
     1981 gccctcggcc tgttcgatgg tcggggtgat cgacccgtcg acgcactccg gccaggtcgg
     2041 gcacccgagg ccgctgccgg tcacccggac cagcccgccc gtgaccacga tcgcgacctc
     2101 ggcgacgagg ttggccagca ggatgggcga gagccaccgg gaaccgggcc gcagggcctg
     2161 atcgctacga gggctgccgg tcatcgaact cgtgctcgtg ctggtcacgg ggtcaaggct
     2221 acggcgcggg ccgcaggctt cgacgcgccg ggtggctcag tccgaccacc ggaaggtccg
     2281 gctcgcgagc accgcagcac cgacgcccca gaccacgagc accgcccact gggcgagcgg
     2341 ccacccaccg tcgacgagcg ccaggcgcat cgcgtcgccg agggcccctg acggcagggc
     2401 cgcggccacg ggagcgagga tgccgggaag cgactcggtg ggagcgagca gtcccagccc
     2461 caacagcagg acccagacga ggttggcgac cgcgaggacg gcttcggcgc gcagcgtgcc
     2521 ggcgagcagc agggccaggg cgacgaagca ggcgctgccg agcaggaggg tcagcgcggc
     2581 gggcaccagc ccagccggct gggcgcgggc ccccagggcc acaccgatcg cgccgatcac
     2641 gacgacctgg acgccgagga ccgacagcac cgccagcgcc ttcgcggcca gcagtccact
     2701 gcggcccaag ggggtggtgc ccagcagccg caggaccccg gcgcgccggt cgaacccggt
     2761 gccgatcgcc tgaccggtga aggcggtcga gaccacgcac agggcaagcg ccccggcgaa
     2821 ggccaggtcg gcgcgggcgc cgggcccgag gtcggggaca gaggtcaggg tcaggccgac
     2881 gagggcgagt gagggcagga tcagcgacac gagcagctgc tcaccgttgg cgagcagcat
     2941 ccgggtctcg aacgcagccc gagacaggat ccgggcccgg gctggcgcgg ctcccggtgt
     3001 caacgcggcg ctcatcgggt ggcctgcgcg gtgagctcga aatagacggc ctcgagggtg
     3061 cgaccggcgc gcacctggtc cagcgggccg tccgccacga ctgtgcccgc cgcgaggatg
     3121 acgacgtggt cggcgagccg ctcggcctcc tcgaaggagt gggtggtgac gacgacgcag
     3181 gcgccgcgtg ccgcctcgct gcgcacgagg tcccagacgt cgagccgggc atgagggtcc
     3241 aggccggcgg tgggttcgtc gaggaagagc acgtcgggcc gggggagcag cgcggcggcc
     3301 agggccacgc gctgccgctg gccccccgac atccgccgca ccgtggtgcg ggcgaaggcg
     3361 tcgatgccga gcctggtcac cagctcgtcg gggtcgaccg gcgccgcgta gagcgccgcg
     3421 aggtggcgca gcagcggcac gggtcggctc gtcgtcggca gcccaccctc ctggagcatg
     3481 accccgaccc gggcgcggtg ctgcggtcca gccccccacg ggtcgacgcc gaggacctgc
     3541 accgtgccgg catccgggcg ttgcagccct tcgaggcatt cgatcgtcgt cgtcttgccg
     3601 gcgccgttcg ggccgaggac ggcggtgatc tggccggcac gcgcacccca ggtggcgccg
     3661 tcgacggccc gcaccccgcc gaaggacttg cgcagatcgc ggacggagac tgcggtggcc
     3721 atgtcgagac atcctagggt ggggcccatg acgcgcatcg accgggaggc cgcccccgcc
     3781 acagccatcg ccgcactccc ttccgtcgtc gcgctgctcg ctggccgtcg gggcctggcg
     3841 gcgggactcg cggtgctccc tgcggcgatc gcggcgttct tccgtgaccc cgatcgaacc
     3901 ccggatcacc gcccggcgcc catcgacgac gtcctgtcgc ccgctgacgg caaggtcatg
     3961 tacgtcggcc ccggtcagga cctcgcggca cctgaggggg agtggcagca gatcagcatc
     4021 ttcctgtcgg ccttcgacgt gcacgtcaac cgggcgccct acggcggacg cgtcaccgcg
     4081 gtcgacttcc gcctcggcaa gtggctggct gcctacaagc acgagagcgc ccacctcaac
     4141 gagcgcagcg acatcaccgt ggaacgcgag gtcggcgggc aggtccgtcg ggtgcacttc
     4201 cgccagatcg tgggcctcat ggcgcgtcgc gtcgtgactc gggtgtccgt cggtgacgag
     4261 atcgccaccg gccagcggat cggcctgatg aagttcgggt cgcggatgga cgtcttcgtg
     4321 ccgccggagt gcgacctgct cgtcaccgcc ggtgaccggg tgatcgcggg ggagaccgtc
     4381 atcgcccggt ggccggcgac agtgacgtcg gtgacctcgt gagcactccc gcagggggtc
     4441 ggcgccgcat cctcgaccgc tggcgcctgg tgtctcccag tggcgcccag gtggtgtcgc
     4501 tgcggcagct gcccaagcgc gagcgggccc ggctgatggc tccgtcgatg ttcaccacac
     4561 tcaacatggt gagcgggctg tcctcggtgc tgctcgcctt ccgtggccac ttcaccatcg
     4621 ccgccgccct catcggcgtc tccatcgtgc tggacatcgc cgacggctac gtcgcccggt
     4681 tgatcggggc cacctccccg ttcggcctcc agctcgactc gctggccgac ctcatctcct
     4741 tcgggctcgc ccccgcgatc ctcgtccaca cctgggccct ggacgagtgg ccgatcctcg
     4801 cgtggctcgt cgcgttcctg tggctggcct gtgccgcgtt ccgtctggcc cgcttcaacg
     4861 tgaccatcga cccggctgcc gacaagcggt acttcgtggg gctcccgtca ccgggtgccg
     4921 ccgccgtcgt catggcgacg atcttcgcgc tcgactcgcc ggactggggc cccggctcag
     4981 gaccgtggcg tgcctggttc ccgctcgtcg tgagcgtggt cccggccctg ctcatggtca
     5041 tgaccgtgcg cttccggtcg ttccgcaacc tgctcgcacc gcagacccgg ggcgctcggt
     5101 tcaccaccgc ggccgtcgtg gtcgccatcc tcgtcgggct ggccctggca ccgggcctca
     5161 ccgcggtggt cgtcgcgtac ggctacgtcc tgaccgcccc gctcggcgtg ctgagcgccc
     5221 ccctgcgcga gcgctttctc ggcgcggagt cgatcgcgcc accgcgcacg aggatgccgt
     5281 ccgtcttcct cccgctcgag gaggacgacg aggtcgacgg cgcccaggca gccgccgtcg
     5341 acggccttga cgaaggttag gccacccttc cttggacgga tctccggtaa ttacctaaca
     5401 ttgctgtgtg gttaatgccg agacgcaggt catcgagtcg cgtacccgcg accgggtgct
     5461 gcgtgtcgtg agccaggagg ggccggtcag catcgcggcc ctcgtcgcca gactgggtct
     5521 caccgagacc gcgctgcgtc gccacgtcga cgccctgcac ggcgagggac tcctcgaggc
     5581 ccgtgaggcc agtggcccca agcgtcgcgg ccggcccgcc agggagtggg tgctcaccga
     5641 ccagggccac cgggcactgg tcagcgacta cgaccacctg gcgggtgagg cgctgcgctt
     5701 cctgggtgcc accgccgggc cggatgccgt cacggcgttc gccgagcacc gcgccgccga
     5761 cctcgagaac cgctacgccg agcgcatcgc gaccgcaggg gatgactccg cgtcccgcgc
     5821 caccgcgctc gtcgaggcac tcaacgctga tggcttcgcg gcatccgctc gtcccgtggg
     5881 ggccgcggac ggcccgctca ccggggtcca gctctgccag gggcactgtc ccgtccagca
     5941 cgtcgccgcc gagttcccgc agttctgcga ggccgagacc gaggcgttct cacgcctgct
     6001 cggcgtgcac gtccaacgac tcgcgaccct cgcccacggc gaccacgtct gcaccacctt
     6061 cgttccaacc ccgaccacct ccaccccgac cagcgagagg gcctcgtcat gacgagccac
     6121 atcgaagagc tcaaccccgg tctcaagggc ctcggcacct acgagtacgg ctgggccgac
     6181 gccgacacgg cgggcgagac ggcccgtcgt ggcctcaacg aagaggtcgt cgcggacatc
     6241 tcggcgcgca agtcagagcc gcagtggatg ctcgacctgc ggatgaagtc gctgcgcctg
     6301 ttcggcaaga agcccatgcc gagctggggc agcgacctca ccggcatcga cttccagaac
     6361 atcaagtact tcgtgaagtc caccgagaag caggccacct cgtgggagga cctgcccgag
     6421 gacatcaaga acacctacga ccgcctcggc atccccgagg ccgagaagca gcgc
//
LOCUS       DAOSOC010000140         6472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12034_length_6472_cov_8.474209, whole genome shotgun sequence.
ACCESSION   DAOSOC010000140 DAOSOC010000000
VERSION     DAOSOC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12034_length_6472_cov_8.474209"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PL014_10150"
     CDS             complement(<1..305)
                     /locus_tag="PL014_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HOT57496.1"
                     /translation="MAAAAPAGEPLEEVPTAATPAAAAGAVPACHSCGGAVADDGYCT
                     ECGTAAVKPRDHFTEQPAAWVAAVCDRGIRHHRNEDAVALAASSSPGERAVLVVCDGV
                     "
     gene            complement(446..2704)
                     /locus_tag="PL014_10155"
     CDS             complement(446..2704)
                     /locus_tag="PL014_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT57497.1"
                     /translation="MSTTACAEPGCTGSILDGYCDVCGSPAAPTSSAAPTSNAPPAGA
                     ASTATAPAGVAHSPSTVSRASNRLASTALGSARAGAGGSSVTRRVGTSSTRLRGARLG
                     AGLTTIPPVAAVDAAKAVLENPMVPEDRRTCPSCGSPVGRSRDGQPGRTEGFCPTCRN
                     PFSFTPKLKPGDVVGGQYVVAGAIAHGGLGWIYAARDRNVSDRWVVLKGLLNSGDPDA
                     LAAAIAERQFLAQVEHPLIVEIYNFVTHEGAGYIVMEYVGGTSLKQLLKARMKEAGGA
                     YNPLPVDQAIAFVLEVLPAFSYLHDLDLVYCDFKPDNVIQVGDAVRLIDLGGVRRIDD
                     ADSAIYGTVGYQAPEVAEVGPSVASDIYTIGRTLVVLAMEFRGYQSTYVSSLPPLADT
                     PLFQEYDSFYRLLLKACAPDPADRFASADELRVQLLGVLREVVAQHRRGAAEHSTSSL
                     LFDVPTVADDSLNWQDLPGLRPDEGDPQLSWLRTVSIDDPLQRLEALQGAPEISAEVL
                     LARARAGLEAAQGARVDDAVQTLLANDPWDWRAVWMSGLLALARGDTTQAQSAFNAVY
                     GQVPGELAPKLALAFACESGGEADVAESLYTVCARTDANYIAPAAFGLARIRAARGDV
                     AGAVRALDLVPGTSRAFTRARRRRAGLLASSGGGLPSLAQALDSIDNLTIDPTDRSRF
                     RVEVFSEALELVQGGGGDSTIRLGGHPASEPALRDGLESALRELAAITDDRDTKVALV
                     DRANGVRRWTLR"
     gene            complement(2701..>3011)
                     /locus_tag="PL014_10160"
     CDS             complement(2701..>3011)
                     /locus_tag="PL014_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57498.1"
                     /translation="LDAVGRALTIAQDAYASALAERDELRGRLEAYAAKATLAAGGSG
                     TALLADLDELAGRVRETVDASPADLVRARALMAAYQAYLGALPTTGGGSIHQTRGGHR
                     "
     assembly_gap    3012..3111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3257..4285)
                     /locus_tag="PL014_10165"
     CDS             complement(3257..4285)
                     /locus_tag="PL014_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT57499.1"
                     /translation="MSRETSRAGAALALVAAVTLAAACSSAGAYDPTPLYTPSPTASP
                     SATPSPSASPSPAPTGTDVVTANCLASYEPLKPMPAPGAMPAGSTMREIQQRGRLIAG
                     VSADTLTLAARNPINGRIEGFDIDMIRAVSQAIFGSPDKVELRVITAAQRLPALQDGS
                     VDIVARNMTITCARWKDIAFSTEYYRSGQKVMVRLGERTDSGGEITGIRDLVGKKVCA
                     PNGSTSMDKLRTFEGVEAVGASTHTGCLVLFQQGAVDAITGDDTVLAGLAAQDPFVEV
                     VQGAAFTAEPYGLGVNQENVDFVRFVNGVLADMKADGRWKASYTTWLAEALGPAPKPP
                     APVYGRNP"
     gene            complement(4378..5769)
                     /locus_tag="PL014_10170"
     CDS             complement(4378..5769)
                     /locus_tag="PL014_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57500.1"
                     /translation="MTTPTSAMATPPPPPTPSAAAAAATAASAGRPAATPAPPAPPAG
                     APAPSGAARSALTGLLAGTPGRLRALGVLGVVAALVFGLGAAQAFRSASGAVDRAAAN
                     ADQVVRIQAIQTNVVQADAVATNAFLVGGLEPASQREAYTTAIATASALIAEAAQNQP
                     ADGAALAALNTELVAYAGQIEIARANNRQGLPIGAQYLRSASADLRAQALPLLSNLAQ
                     ANDERVALEFNRAGQALWWLMVPGLLALAALGAALTWIARHSHRYVNVPLAGAALVVL
                     VTLVGGAAGLLGVKADVDTTRDGEYAATRAVAAARTAAFDAKANESLTLIARGSGAAF
                     QEAWAASSEAVTTQFATLDANLVATGLDEQLWADYVAAHEEIRALDDSGDWDGAVDLA
                     TGTGAGTGNTTFAAFDEDSAQVLSDVADRTASSLADTGGWLPIGGILSVLVGLLAAGL
                     SWWGVSQRLEEYR"
     gene            complement(5766..>6472)
                     /locus_tag="PL014_10175"
     CDS             complement(5766..>6472)
                     /locus_tag="PL014_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT57501.1"
                     /translation="VAGYLGQTATKTAEVVASAAGGVLFIDEAYSLTSSGPSGDQYGR
                     EAVDTLVKEMEDRRDDLVVIVAGYPAPMETFIGANPGLASRFRTTIEFEDYTDDELVA
                     ILLHVAEGADYEVLPEAVERFREVLARAPRGDGFGNGRFSRNALEAAIGHHAWRLRKV
                     DNPTLEQLRQLVAKDFDTEPQDDTAPDDMPADDAAPDDMPAVASAPPSSEDLAVDGAD
                     PDLPASHEPTSSGGTP"
BASE COUNT          832 a         2218 c         2419 g          903 t
ORIGIN      
        1 acgccgtcgc acacgacgag cacggcccgc tcccccggcg acgacgacgc cgccagggcc
       61 acggcatcct cgttgcgatg gtggcggatc ccgcggtcgc acacggcggc gacccacgcc
      121 gcgggctgct cggtgaagtg gtcgcgcggc ttgacggccg cggtgccgca ctcggtgcag
      181 tagccgtcgt cggcgaccgc tccaccgcag gagtggcacg cgggcactgc tcccgctgcc
      241 gcggccggcg tcgccgccgt cggcacctcc tccagcggct cgcccgctgg tgccgccgct
      301 gccacgccgg cctccgcacg ggtgccagcg gtcgcaggtg tgccagcgct tgcgggggtg
      361 cccaggtcgg cgccgcagcc ctcgcagaac cgctcgcccg cgctcacgtg gctgccacac
      421 gacgggcagg gggtcgcctc atcggtcatc gcagggtcca ccgccgcacg ccgttggccc
      481 gatcgacgag cgccaccttc gtgtcccggt cgtcggtgat cgcggcgagt tcgcgcaggg
      541 ccgactcgag gccgtcgcgc agcgccggct cggatgccgg atgcccaccg aggcggatcg
      601 tggagtctcc gcccccgccc tggaccagct ccagggcctc ggagaacacc tcgacccgaa
      661 agcgtgaccg gtccgtcggg tcgatggtga ggttgtcgat gctgtcgagc gcctgggcca
      721 gggagggcag gccgccgccg gacgaggcca gcaggccggc ccggcgccgt cgagcccggg
      781 tgaacgccct gctcgtgccg gggacgaggt cgagggcgcg cacggctcct gcgacgtcac
      841 cgcgcgcggc gcggatgcgc gccaggccga aggccgcggg agcgatgtag ttggcgtcgg
      901 ttcgggcaca cacggtgtag agcgactcgg cgacatcggc ctctcccccg ctctcgcagg
      961 cgaaggccag cgccagtttc ggagccagct caccggggac ctgcccgtag acggcgttga
     1021 aggcgctctg cgcctgcgtg gtgtcacctc gggccagcgc gagcagaccc gacatccaca
     1081 ccgcccgcca gtcccacgga tcgttggcca gcagggtctg cacggcatcg tccacgcgcg
     1141 caccctgggc tgcctcgagg ccggccctgg cccgggccag cagcacctcc gcgctgatct
     1201 cgggggctcc ctggagcgcc tcgagccgct ggagcgggtc gtcgatgctg accgtgcgca
     1261 gccacgacag ctgcgggtcg ccctcatcag gtcgcagacc gggcaggtcc tgccagttca
     1321 gggagtcatc ggccacggtg gggacgtcga agagcaacga cgacgtcgag tgctcggccg
     1381 caccgcggcg gtgctgcgcc accacctcgc gcagcacacc gagcagctgc acgcgcagct
     1441 cgtcggcaga ggcgaagcgg tcggccgggt ccggggcaca ggccttgagc agcaggcggt
     1501 agaaggagtc gtactcctgg aacaacgggg tgtcggcgag cggcggcagg ctgctcacgt
     1561 aggtgctctg gtagccccgg aactccatcg cgagcacgac gagggtgcgg ccgatggtgt
     1621 agatgtccga ggccaccgac ggcccgacct ccgcgacctc gggggcctgg tagccgacgg
     1681 tgccgtagat cgcggagtcc gcgtcgtcga tccgccggac cccaccgagg tcgatcaggc
     1741 gcacggcgtc acccacctgg atgacgttgt ccggcttgaa gtcgcagtag acgaggtcga
     1801 ggtcgtgcag gtaggagaac gcggggagca cctcgagcac gaaggcgatc gcctggtcga
     1861 cgggcagcgg gttgtaggcc ccacccgcct ccttcatccg cgccttgagc aactgcttga
     1921 gcgaggtccc gccgacgtac tccatgacga tgtagccggc accctcgtgg gtgacgaagt
     1981 tgtagatctc gacgatgagc gggtgctcga cctgcgcgag gaactgccgc tcggcgatgg
     2041 ccgcggcaag ggcatcgggg tcacccgagt tgagcaggcc cttgaggacg acccaccggt
     2101 cggagacgtt gcggtcgcgg gcggcgtaga tccagcccag gccaccgtgg gcgatcgccc
     2161 cggcgacgac gtactgaccg ccgacgacgt cgccgggctt gagcttcggg gtgaaggaga
     2221 aggggttgcg gcacgtgggg cagaacccct cggtgcgacc gggctgcccg tcgcgggagc
     2281 gacccaccgg tgacccgcac gaggggcagg tgcgccggtc ctcggggacc atcgggttct
     2341 cgagcacggc cttggccgcg tcgactgcgg cgaccggggg gatcgtcgtc agtcctgcgc
     2401 cgagacgggc cccgcgcagg cgggtcgacg aggtcccgac cctgcgggtg accgaactgc
     2461 cgccggcccc ggcgcgcgcg gagcccaggg cggtggacgc gaggcggttc gatgcgcgcg
     2521 acaccgtgga cgggctgtgg gccaccccgg ccggtgccgt ggcggtggat gccgcgccgg
     2581 ctggtggggc gttcgaggtg ggtgcggcac tcgaggtggg tgcggcaggc gagccgcaga
     2641 cgtcgcagta gccgtccagg atcgagcccg tgcagccggg ctcggcgcag gccgtcgtgc
     2701 tcatcggtgt cctccccgtg tctggtggat tgatccgccg cccgtggtcg gcagcgcccc
     2761 caggtaggcc tggtaggccg ccatgagggc ccgtgcccgg accaggtcgg cgggcgaggc
     2821 gtccacggtc tcccgcactc gcccagcgag ctcgtcgagg tccgccagga gcgccgttcc
     2881 cgacccgccc gcggccaggg tggccttggc ggcgtacgcc tcgaggcggc cacgcagttc
     2941 gtcccgctcc gcgagggccg aggcgtacgc gtcctgggcg atggtcaggg cgcgcccgac
     3001 ggcatccaac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcctcgtcg
     3121 atgtggtcga gctcgcgctt gcgctcctcg cgccaggtgc cgagggcgct gaggtactcc
     3181 agcgcgtcct gcgcctgcag cggccgcccg agtcgaccgg gcgcccgggg tgcggtggcc
     3241 ttcctcgcca cggagatcac gggttccgcc cgtagacggg ggccggtggc ttgggggccg
     3301 ggccgagggc ctcggcgagc cacgtggtgt agctggcctt ccagcggccg tcggccttca
     3361 tgtcggcgag caccccgttg acgaaccgca cgaagtcgac gttctcctgg ttgaccccca
     3421 gcccgtaggg ctcggcggtg aaggccgccc cctggacgac ctcgacgaag gggtcctgtg
     3481 cggccaggcc ggcgagcacc gtgtcgtcgc cggtgatggc gtcgaccgcc ccctgctgga
     3541 agagcacgag gcacccggtg tgcgtgctcg cgccgaccgc ctcgaccccc tcgaaggtgc
     3601 gcagcttgtc catgctcgtc gacccgttcg gagcacagac cttcttgccg acgaggtcgc
     3661 ggatgccggt gatctcacct ccgctgtcgg tgcgctcccc gagccggacc atgaccttct
     3721 ggccggaacg gtagtactcg gtggagaagg cgatgtcctt ccaccgggcg caggtgatcg
     3781 tcatgttgcg ggcgacgatg tcgacgctgc cgtcctggag cgcgggcagc cgctgcgccg
     3841 cggtgatcac gcgcagctcg accttgtccg ggctgccgaa gatcgcctgt gacaccgcgc
     3901 ggatcatgtc gatgtcgaag ccctcgatgc ggccgttgat cgggttgcgc gcggccagcg
     3961 tcagcgtgtc ggccgagaca ccggcgatga ggcggccacg ctgctggatc tcgcgcatcg
     4021 tcgagccggc gggcatcgca cccggtgcag gcatgggttt gagcggttcg taggaggcca
     4081 ggcagttggc cgtgacgacg tcggtgcccg tcggcgctgg cgacgggctg gccgacggcg
     4141 acggcgtggc cgaggggctg gccgtgggtg acggggtgta caggggggtc gggtcgtagg
     4201 cccctgcgct ggagcaggca gccgcgagcg tcacagcggc gacgagggcg agggcggcgc
     4261 ccgccctgga cgtctccctg gacatcgccg tcgccgtcac cctggaggtc gttgcgaagg
     4321 tcgccgtggc ggtggccctg acggcggcgc tgctgcgacc ggctcgcgtc tcgcggctca
     4381 tcggtactcc tccagtcgct gcgagactcc ccaccaggac aaccctgccg ccagcaaccc
     4441 cacgagcacg ctcaggatcc caccgatggg cagccagccc ccggtgtcgg cgagggagga
     4501 ggcggtgcgg tccgcgacgt cgctcagcac ctgggcggag tcctcgtcga aggccgcgaa
     4561 ggtcgtgttc ccggtgccgg caccggtgcc ggttgccagg tccacggcgc cgtcccagtc
     4621 accggagtcg tccagggccc ggatctcctc gtgggcggcg acgtagtcgg cccacagctg
     4681 ctcgtccagg ccggtggcga cgaggttggc atcaagcgtg gcgaactgcg tcgtgaccgc
     4741 ctcggacgac gccgcccagg cctcctggaa ggctgccccc gacccacgag cgatcagggt
     4801 gaggctctcg ttggccttgg cgtcgaaggc ggccgtgcgc gcggcggcga cagcgcgggt
     4861 ggcggcgtac tcgccgtcgc gagtggtgtc gacatcggcc ttgaccccga ggagcccggc
     4921 ggccccgccc acgagcgtga ccagcacgac gagggccgca ccggccagcg gcacgttgac
     4981 gtacctgtgg gagtgccgag cgatccaggt gagggctgcg ccgagtgccg cgagggccag
     5041 caggccgggg accatcagcc accacagggc ctggcctgct cggttgaact ccagcgccac
     5101 gcgctcgtcg ttggcctgcg cgaggttgct gagcaggggc agtgcctgcg cccgcaggtc
     5161 cgcgctggcc gagcgaaggt actgcgcgcc gatcggcaac ccctgacggt tgttggcccg
     5221 agcgatctcg atctgacctg cgtacgccac gagctcggtg ttgagggcgg ccagcgcggc
     5281 gccgtcggcg ggctggttct gtgcggcctc ggcgatgagg gcggatgccg tggcgatcgc
     5341 cgtcgtgtat gcctcgcgtt ggctggccgg ctcgaggccg ccgacgagga aggcattcgt
     5401 ggcgacggcg tcggcctgca cgacgttggt ctggatcgcc tggatccgca ccacctggtc
     5461 ggcgttcgcc gcggcccgat cgacggcgcc cgacgccgag cggaaggcct gcgcagcgcc
     5521 gagcccgaac accagggccg cgacgacccc gagcacccct agtgcacgca gccggccggg
     5581 cgtgccggcc agcagccccg tcagcgcgct gcgggctgca ccggacggtg ccggggcccc
     5641 tgccggtggc gcaggcgggg cgggcgttgc ggccggtcgg cctgccgagg ccgcagtggc
     5701 ggcggccgcc gcagcactgg gggtcggggg cggtggcggg gtggccatcg ctgacgtggg
     5761 ggtcgtcacg gggtgcctcc agatgacgtg ggctcgtgtg acgcaggaag gtcggggtcg
     5821 gctccgtcga cggccaggtc ctccgacgac gggggtgcac tggcgaccgc gggcatgtcg
     5881 tccggtgcag catcgtccgc gggcatgtcg tccggtgctg tgtcgtcctg tggctcggtg
     5941 tcgaagtcct tggcgacgag ctgtcgcagc tgctccaggg tcgggttgtc gaccttgcgc
     6001 agccgccacg cgtggtggcc gatcgcagcc tcgagggcgt tgcgagagaa ccggccgttg
     6061 ccgaaaccgt ccccccgagg ggcgcgtgcc aggacctccc ggaaccgctc gacggcctcg
     6121 gggagcactt cgtagtcagc gccctcggcc acgtgcagca ggatcgcgac gagttcgtcg
     6181 tcggtgtagt cctcgaactc gatggtcgtc cggaagcggc tggccaggcc cgggttggcg
     6241 ccgatgaagg tctccatcgg cgccgggtag cccgcgacga tgacgacgag gtcgtctcgt
     6301 cggtcctcca tctccttgac gagcgtgtcg acggcctcgc gaccgtactg gtcgccgctg
     6361 gggccactgc tcgtcaggct gtaggcctcg tcgatgaaga gcaccccccc ggcggccgag
     6421 gcgacgacct cggccgtctt ggtcgccgtc tgcccgaggt agccggcgac ga
//
LOCUS       DAOSOC010000141         6440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12133_length_6440_cov_7.300705, whole genome shotgun sequence.
ACCESSION   DAOSOC010000141 DAOSOC010000000
VERSION     DAOSOC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12133_length_6440_cov_7.300705"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PL014_10180"
     CDS             complement(<1..838)
                     /locus_tag="PL014_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT57502.1"
                     /translation="MAPLTFDAYSAQWLRERDLKPRTREGYEHLMRSYLSPVLGPLVI
                     DTITPSVVRTWWSQLPADKPTVRARSYALLKAVMNTAVADEVIDSNPCRIRGAANTPR
                     AREIRPASIDELAVIVEAIPSRHRALVLLCAWCALRSGEVLELRRKDVDIAAGTVRVV
                     RAVSWVGGQPIVGTPKSAAGTRVVSIPPHIVPAVAHHLDTMTAASPDALLFPGRDGVS
                     HLQPSTLHRNWRVAREAAGRPDLRVHDLRHTGATMAARAGATLAELQQRLGHSSVSAA
                     LRY"
     gene            complement(1200..1272)
                     /locus_tag="PL014_10185"
     tRNA            complement(1200..1272)
                     /locus_tag="PL014_10185"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1237..1239),aa:Thr,seq:tgt)
     gene            complement(1345..2829)
                     /locus_tag="PL014_10190"
     CDS             complement(1345..2829)
                     /locus_tag="PL014_10190"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOT57503.1"
                     /translation="MTTPPKRHPARVPGWRRGGFMVGEEPTEPMPMNDLLRGTPYRDI
                     RVPGEAAEDAQVRRALDLAARVGELMLRCGAGAPQVVGSVAAVAAAAGVDVIEVDITL
                     QSLLVQASSSSGRPHTLLRVVRRTRHDYARLAAVHELVEGLADGSIDSRDADRLLREI
                     KRTPRRFSVTAVSVASAVLASSVAVMIGASPAAAVITVLVVLVVTGVNRVHSGFDLPE
                     FYGNAINALVATVLAGLAYAVATLVGSPLGEQDFAFIVAGGIVAMLPGRTMAAAIEDV
                     LFGFPLTGAGRLLSVLVALMGLIIGIGAGLKVMLWITTVLDTQFVSPTVLELRTDQGA
                     LVTALLGALFVGLSAAVTVQSRARLILPTGLLSVGAATIGAILQRSLEFGVITATGFA
                     AIALGAVGRVVAARLRVPSMVIIVPASFALLPGLTIFRGLYELVAVGGASAGSLTIQS
                     GLTTLLSAGAVLLAIATGTVFGEILTSRWDTSVRGERPKGSDGG"
     gene            complement(2855..3595)
                     /locus_tag="PL014_10195"
     CDS             complement(2855..3595)
                     /locus_tag="PL014_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57504.1"
                     /translation="MQPQPGYRQHLPAPAPVPGQVAGPAPESESDWDAARQGLGPYAD
                     PRIGRPSRAGTLLALLALLGAVSAAAPVVAAMALLGWIALARTADRSVTSLVMRRHER
                     GARGSDVAVAVASSPWHLVVGTLGALLTAVLPLFVGLCALFATALVLGVATGADVSTG
                     QTVPLVAAGVFAGLTAWWGPGGASLRRGSRSIVRGISPGETMRQVVAAVLLVAAAGIL
                     AVTVTGDGATWWPRTTAPSWVDTAVPAP"
     assembly_gap    3723..3822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3823..4780)
                     /locus_tag="PL014_10200"
     CDS             complement(<3823..4780)
                     /locus_tag="PL014_10200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT57505.1"
                     /translation="MTADAIADGPVPDDAEELEEPREHTRLGQYRLVQRIGEGGMGVV
                     HLALDRHGRAVALKVLRPHIALDPDARTRLAREVNSLSRVSSPFVAPIVDADVEGPRP
                     YIVTRYVPGPSLDEQVADRGLLPPAELHRVAHGLAEALDAIHGAGVIHRDVKPANVLL
                     VDGEPVLIDFGIAHVADDVRLTMAGLVMGTPGYLAPEVISGQDVTAATDWWGWAATTA
                     FAAGGVPPFGRGAMSAVLARVAQGEPDLRAVDPRIEPLLYAALAPDPAQRPHHHEVVA
                     ALESYAHGAPATAAIEVRRVRPQTQVLAIAPTSVMQPAPPAAP"
     gene            complement(4845..5273)
                     /locus_tag="PL014_10205"
     CDS             complement(4845..5273)
                     /locus_tag="PL014_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT57506.1"
                     /translation="MRISDVVRLKGGDVITVRSDASVTELLALLSEHRIGAVVVSDDG
                     EKVHGIVSERDIVRHLHTDGAALLDAPVHQIMTAEVHTCVPEDSLDDLESAMTEKRFR
                     HVPVLSDGKLVAIVSIGDVVKNRIDDLQAERDQLRTYIQS"
     gene            complement(5406..6440)
                     /locus_tag="PL014_10210"
     CDS             complement(5406..6440)
                     /locus_tag="PL014_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT57507.1"
                     /translation="MAEIRSARLADIAAQADVSEATVSRVLNDRPGVADSTRRAVLTA
                     IDVLGYDRPARLRPKSALLVGLVMPELVNPIFPAFAQVIETALAAEGFTPVLCTQTPG
                     GVHEDDYVQMLIDRGVSGIIFLSGQHADATSDPARYQALRERGLPIVLVNGWLEGVDA
                     PFVSNDDVASMDLAVSHLAQLGHTEIGLALGPERYTPVIRKVQGFAAAMHQHLGVEQA
                     HLEQLITHTVFSLEGGAAAAGRLITRGATAVICGSDLMALGAIRQARAMGRSVPADFS
                     VVGYDDSTMMAFTDPPLTTIRQSVDAMGDAAVRALLDDIAGEGPPCAEYVFRPELVVR
                     GSTGAAPPRG"
BASE COUNT          953 a         2280 c         2295 g          812 t
ORIGIN      
        1 ggtacctgag cgccgcggag accgagctgt ggccgagtcg ttgctggagt tcggcgaggg
       61 tcgcccccgc ccgggcggcc atcgtggctc cggtgtggcg gaggtcatgc acccgcaggt
      121 ccgggcgacc ggcagcctcc cgcgccaccc gccagttccg gtgcagcgtg gacggctgca
      181 ggtgggagac gccgtcccga ccggggaaga gcaaggcgtc cgggctcgcg gccgtcatcg
      241 tgtcgaggtg gtgggcaacc gcggggacga tgtgcggcgg gatggagacc acccgcgtcc
      301 ccgccgccga cttcggcgtc cccacgatgg gctggccacc gacccacgac accgcacgga
      361 cgaccctcac cgttcccgcc gcgatgtcga cgtccttgcg ccgcagctcg agcacctcac
      421 cgctgcgcag cgcacaccac gcgcacagca gcaccagcgc acggtgccgg ctcgggatgg
      481 cctcgacgat caccgccagc tcatcgatgg aggccgggcg gatctcccgg gctcgaggcg
      541 tgttcgccgc gccccggatg cgacacgggt tgctgtcgat cacctcgtcg gcgaccgccg
      601 tgttcatgac cgccttgagc agcgcgtagc tgcgagcccg gacggtcggc ttgtccgcgg
      661 gcagctgcga ccaccaggtc cgcacgacgc tgggcgtgat cgtgtcgatg accagcggac
      721 cgagcaccgg gctcaggtag ctgcgcatca ggtgctcgta gccctcccgc gtccggggct
      781 tgaggtcacg ctctcgcaac cactgtgccg agtacgcgtc gaaggtcagc ggtgccacct
      841 tgggcacctt caccggcggc tgccactccc ccgccatcac ctcccacgac gtcgcccgag
      901 ccaaccctcg gcatccccct tggtctcgaa cgtctgcgga gcgggccgcc ggaccaggtc
      961 cgggcccacg aaacttgcct gccagcgccc cgaaggcagc ttccgaaggc tcccgaaccc
     1021 cctccgggac ttgcccgaag tcatctcgac gcacctgtct gttgccgctg gcgtgcaaca
     1081 tacgtgcaac aacggtggca attcctgtcc atctgcgtcc actcctgacc ccgtggcgac
     1141 gacactgtcg ccgcctcaat agcagcacag gacaggggtt ttcagcaaga actaccttga
     1201 gctggcgaag ggactcgaac cctcaacccc ctgtttacaa gacaggtgcg ctaccaattg
     1261 cgccacgcca gcacgcccga cccgacgcag cggaccggac agccccggag tctacggccc
     1321 ccaccggaca cggcgagaac ggcgtcagcc gccgtcgctg cccttcggcc gctccccgcg
     1381 caccgaggtg tcccaccgcg aggtgaggat ctcgccgaag accgttcccg tcgcgatcgc
     1441 gagcagcacg gccccggccg acagcagggt cgtcaggccc gactggatcg tcagcgaccc
     1501 cgctgacgcg ccgccgaccg ccaccagttc atagaggccg cggaagatcg tcagacccgg
     1561 gagcagcgcg aacgaggcgg gcacgatgat gaccatcgac ggcacccgca agcgcgcggc
     1621 cacgacccgc ccgacggcac cgagggcgat ggccgcgaac ccggtggccg tgatgacccc
     1681 gaactccagg gagcgctgca ggatcgcccc gatcgtggcg gcacccaccg agagcagccc
     1741 ggtcggcagg atgagtcgcg cccgactctg cacggtcacc gcggcgctga gcccgacgaa
     1801 cagggcgccg agcagagcag tgacgagcgc gccctggtcc gtgcgcagtt cgagcaccgt
     1861 cggcgagacg aactgggtgt cgagcacggt cgtgatccac agcatgacct tcaggccggc
     1921 gccgatgccg atgatcagcc ccatgagcgc gaccagcaca gacagcaacc gccccgcccc
     1981 ggtgagcggg aacccgaaca gcacgtcctc gattgccgcg gccatcgttc gcccgggcag
     2041 catggcgacg atgccgcccg cgacgatgaa cgcgaagtcc tgctccccga gcggggaccc
     2101 gacgagcgtc gccacggcat acgccaggcc tgccaacact gtggccacga gcgcgttgat
     2161 cgcgttgccg tagaactcgg gcaggtcgaa cccggagtgc acccggttca cccccgtcac
     2221 gacgagcacg acgagcaccg tgatgacggc ggccgccggg ctggcaccga tcatcaccgc
     2281 gacggacgac gccagcaccg cgctggccac ggataccgct gtgacggaga agcgccgcgg
     2341 ggtgcgcttg atctcgcgca gcaggcgatc ggcatcccgg gagtcgatcg acccgtccgc
     2401 gaggccctcg acgagttcgt ggaccgcggc caggcgcgcg tagtcgtgcc gggtgcgccg
     2461 cacgacgcgc agcagggtgt gcggtcgccc gctcgagctg ctggcctgga cgagcaggga
     2521 ctgcaaggtg atgtcgacct cgatgacgtc gacgcccgcc gcggccgcca ccgccgcgac
     2581 gctgcccacg acctgcgggg cgcccgcacc acagcgcagc atgagttcgc cgacccgcgc
     2641 ggcgaggtcg agcgcgcgcc tgacctgtgc gtcctcggcc gcctcaccgg ggacccggat
     2701 gtcgcggtac ggcgttccgc gcagcaggtc gttcatcggc atcggttcgg tcggctcctc
     2761 gcccaccatg aagccgccgc gacgccagcc cggcaccctg gccggatgcc gtttgggggg
     2821 cgtcgtcatc gcgtcaccgt cgtcgtcgcg ggcgtcacgg cgccggaacc gccgtgtcga
     2881 cccacgaggg agcggtggtg cgcggccacc aggtcgcgcc gtcacccgtc accgtcacgg
     2941 cgaggatgcc ggccgcggcc acgagcagga ctgccgccac gacctgacgc atcgtctccc
     3001 cgggcgagat gccgcgcacg atgctgcggc tgccccgccg gaggctggcg ccgcccggcc
     3061 cccaccaggc cgtgagcccc gcgaagacgc cggccgccac cagggggacg gtctggcccg
     3121 tgctgacatc ggccccggtc gcgaccccga gcacgagcgc cgtcgcgaag agggcacaca
     3181 gccccacgaa cagcgggagc accgcggtga gcagggcacc cagcgtgccg acgaccaggt
     3241 gccacgggct gctcgcgacg gccacggcga catcgcttcc gcgggctcca cgttcgtgac
     3301 ggcgcatgac gagggaggtc accgagcggt cagccgtgcg cgcgagagcg atccagccga
     3361 gcagggccat cgcggccacc accggggcgg ccgccgacac cgccccgagc agcgccagca
     3421 gggcgagcag ggtgcccgcc cggctcgggc gaccgatccg ggggtccgcg tagggaccca
     3481 gaccctgccg agcggcatcc cagtccgact ccgattccgg tgccgggcca gccacctggc
     3541 ccggcaccgg ggctggcgca gggaggtgct ggcggtagcc gggctgaggt tgcatcggcg
     3601 cgggcaccgg cgcaggaacc ggcgcgggcg cccactgccg cggtggcggc gcgggaaccg
     3661 gcgcggacgc ccactgcggc ggtggcggtg cgagcacggg cgcagcggga ggccgtggcg
     3721 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgggcgca gcgggaggcg
     3841 ccggctgcat gacgctcgtc ggggcgatgg cgagcacctg cgtctgcggg cgcacgcggc
     3901 gcacctcgat ggccgccgtc gccggggcgc cgtgggcgta gctctcgagc gccgcgacga
     3961 cctcgtggtg gtgcgggcgc tgggccgggt cgggcgcgag cgccgcgtag agcaggggtt
     4021 cgatgcgcgg gtcgacggca cgcaggtcgg gctccccctg ggccacccgg gccagcacgg
     4081 cgctcatggc cccacggccg aacggcggca ccccgcccgc ggcgaaggcg gtcgtcgcgg
     4141 cccaccccca ccagtcggtg gctgcggtga cgtcctggcc gctgatgacc tcgggggcga
     4201 ggtagccggg cgttcccatg accaacccag ccatcgtgag tcggacgtcg tcggcgacgt
     4261 gggcgatgcc gaagtcgatg agcacgggtt cgccgtcgac gaggaggacg ttcgccggct
     4321 tgacgtcacg gtggatgacg cccgcgccgt ggatggcgtc gagggcctcg gcgaggccgt
     4381 gggccacgcg gtgcagctcg gccggtggca ggagcccccg atcggcgacc tgctcgtcga
     4441 gcgaggggcc ggggacgtag cgcgtcacga tgtagggacg cgggccctcg acgtcggcgt
     4501 cgacgatcgg ggcgacgaac gggctgctca cccggctgag ggagttgacc tcgcgagcca
     4561 ggcgggtccg cgcatccggg tcgagggcga tgtgcggtcg cagcaccttc agggcgaccg
     4621 ctcgaccgtg ccggtccagg gcgaggtgca ccacgcccat cccgccctcg ccgatgcgct
     4681 ggacaaggcg gtactgcccg aggcgcgtgt gttcgcgggg ttcctcgagc tcttcggcat
     4741 cgtcaggcac ggggccgtcg gcgatcgcgt cggcggtcat ggtgaccacg gtaggcgctc
     4801 agtctgggtc tgggctgcat gcgcgaccca gtgcccctgc ggcgtcagga ctggatgtag
     4861 gtgcgcaact ggtctcgctc ggcctggagg tcatcgatcc ggttcttcac gacgtcgccg
     4921 atgctgacga tcgcgacgag cttgccgtcg gaaagcaccg gcacgtggcg gaaccgcttc
     4981 tccgtcatcg ccgactcgag gtcgtcgagg ctgtcctcgg gcacgcaggt gtgcacctcg
     5041 gccgtcatga tctggtgcac cggggcgtcg agcagcgccg caccgtcagt gtgcaggtgc
     5101 cgcacgatgt cgcgctcact gacgatgccg tgcaccttct caccgtcatc gctgacgacg
     5161 acggcaccga ttcggtgctc cgagagcagc gccagcaact cggtcacgga ggcgtccgaa
     5221 cgcaccgtga tgacgtctcc acccttgagc ctgaccacgt ccgagatcct catgagccga
     5281 cgataggtgc gcgcgggggc gacgcgccag tgcttgaccg cgtgacggac cactgattcc
     5341 gaagtggcga cgtccatgac accgaacgcg cacagcgcaa cgacgcgaac ggcatccgga
     5401 tgccgtcagc ctcgcggcgg tgccgcgccc gtcgacccgc gcacgacgag ttccgggcgg
     5461 aagacgtact ccgcgcacgg gggcccctcc ccggcgatgt cgtcgagcag cgctcgcacg
     5521 gcggcatccc ccatggcgtc gacggactgg cggatcgtcg tcagcggggg gtcggtgaac
     5581 gccatcatcg tggagtcgtc gtagccgacc accgagaagt cggccggcac gctgcgcccc
     5641 atggcccgcg cctggcggat cgctccgagg gccatgaggt cggagccgca gatgacggcc
     5701 gtcgcaccgc gggtgatgag gcgaccggcc gcggccgcac ccccttcgag gctgaagacc
     5761 gtgtgggtga tcagctgctc gaggtgcgcc tgttcgacgc cgaggtgctg gtgcatggcg
     5821 gcggcgaacc cctgcacctt gcggatcacg ggggtgtagc gctcgggccc cagggcgagg
     5881 ccgatctcgg tgtgtccgag ctgggcgagg tggctcacgg cgaggtccat cgaggcgacg
     5941 tcgtcgttgg agacgaacgg ggcgtcgacg ccctcgagcc agccattgac cagcacgatc
     6001 ggcaggcccc gctcgcgcag ggcctggtag cgggccgggt ccgacgtcgc gtcggcgtgc
     6061 tgcccggaca ggaagatgat gccggagacg ccgcggtcga tgagcatctg cacgtagtcg
     6121 tcctcgtgca ccccaccggg ggtctgggtg cacagcaccg gggtgaaccc ctcggccgcg
     6181 agcgcggtct cgatgacctg ggcgaacgcg gggaagatgg ggttgacgag ttcgggcatg
     6241 acgagcccga cgagcagcgc cgacttcggc cgcagcctgg ccggccggtc gtaaccgagc
     6301 acgtcgatgg cggtgagcac ggcgcgccgg gtggagtcgg ccacgccggg acggtcgttc
     6361 agcacgcggc tgaccgtcgc ctcgctgacg tcggcctggg cggcgatgtc ggcgagtcgc
     6421 gcagagcgga tctcggccac
//
LOCUS       DAOSOC010000142         6383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12278_length_6383_cov_10.588654, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000142 DAOSOC010000000
VERSION     DAOSOC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12278_length_6383_cov_10.588654"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PL014_10215"
     CDS             <1..843
                     /locus_tag="PL014_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT57508.1"
                     /translation="HEFIATGHEDQKFGFSLAGGQAFEAATRILASPDVLDLLGLHSH
                     IGSQIFDTGGFEMAAHRLVALHARIAEEHGHHCPEIDLGGGYGIAYTSQHTPLPASTL
                     AGEMAGILEREFKAVGDGDLSTLPRVSIEPGRAIVGPSTFTLYEVGTVKPVELGGGHT
                     RTYVSVDGGMSDNARPALYEADYSCTLANRRSEAGPMLSRVVGRHCESGDIVVLDEYL
                     PADVQPGDLIAVPGTGAYCRALSSQYNHTPRPPVVAVRGGRSRVLVRRETIEDLLALD
                     VADA"
     gene            873..2228
                     /locus_tag="PL014_10220"
     CDS             873..2228
                     /locus_tag="PL014_10220"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOT57509.1"
                     /translation="MAHETMAHATGAPTEAPGRAPLRVALLGCGVVGSAVATRLVEHA
                     DDLAARVGAPLELVGIAVRRPQRPRPDVPVDPALFTADAEELVTRADIVIEVIGGIDP
                     ARGLILRAMEHGASVVSANKALLAEDGPALYAAADAADVDLYYEAAVAGAIPILRPIR
                     ESLAGDDVRKVLGIVNGTTNFVLDKMDTSGAGFAETVEQAQALGYAEADPTADVEGFD
                     AAAKAAILASLAFHTRVSSADVHREGITEVTAGDIQAAKEMDCVVKLLAICQRVTSAD
                     GSDAVSVRVHPAMIPRSHPLASVRDAFNAVFVEATAAGPLMFYGRGAGGDPTASAVLG
                     DVVAVARHKVAGGRGPGESAYADLPVVDMGQALTRYHISLDVADRPGVLAQVATAFAE
                     QGVSIETVRQQVAAGSGERAKLIVVTHRASDAALSATVDALAGLDSVDDVASVMRVEG
                     D"
     gene            2235..3338
                     /gene="thrC"
                     /locus_tag="PL014_10225"
     CDS             2235..3338
                     /gene="thrC"
                     /locus_tag="PL014_10225"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOT57510.1"
                     /translation="MAHLWRGVIREYAERLPMLEGAPVVTLQEGGTPLIPCEGLSELT
                     GANVFVKFEGLNPTASFKDRGMTTAMSMAKKHGAKAVICASTGNTSASAAAYATKAGM
                     TCAVLVPDGKISMGKLSQAVAHGATLIQVEGNFDDCLTVARKLAEAYPVELVNSVNPA
                     RIEGQKTAAFEVVDALGDAPDIHILPVGNAGNITAYWRGYTEYASVTTGVGGVELPAV
                     ATRTPRMWGYQAAGAAPIVLGHPVDDPETIATAIRIGNPASWAQAEAARDESGGHIDS
                     VTDDEILEGHRLLSSTEGIFVEPGSAASIAGLLKAHRAGIVPRDATIVCTVTGHGLKD
                     PQWALRTADGGDVTPVRVPVDAFSVAVALGLDG"
     gene            3335..4282
                     /gene="thrB"
                     /locus_tag="PL014_10230"
     CDS             3335..4282
                     /gene="thrB"
                     /locus_tag="PL014_10230"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOT57511.1"
                     /translation="MSTTRLRAGVEVEVRVPASSANLGPGFDSIGCALGVWDTCRARV
                     TSTPGLTVTVAGEGAGSVPLDATHLVHRAMVVAWGELGVEPPTGLALDCTNEVPHGRG
                     MGSSATAIVTGIVAAQALADVSAGDAGDAVDLTFANSLASRLEGHPDNASASVYGGFT
                     LSWSDDEQASTTTVHLPVHPDVVPVVFAPDVQLSTAKARSVLPQQVRLADAAANSARS
                     ALLAHAIGFAPEHLLAATKEWLHQEARRPSYAASMSLVDRLRSRGHAAVISGAGPSVL
                     VLARREHVEEVRAHGEAGWRVLTPGIPDHGARVTVLSRA"
     gene            4481..>6383
                     /gene="rho"
                     /locus_tag="PL014_10235"
     CDS             4481..>6383
                     /gene="rho"
                     /locus_tag="PL014_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT57512.1"
                     /translation="MSDTTTLEPSTAGASPRRSGALSAMRLAELQGLASSMGISGTAK
                     MRKGDLVAAIKARQNGESGPAATSEPSARASEPTRGTEPRRSRRATSTGRGVETAPAT
                     EQATPVVAPEQAAQAPVETAPARADAERDVRSDAAEQADGQARGERGAGRTRTRAARQ
                     DRTEARDAQSEDRGDQTASADTGSGEAQGEQNAGAQGGQNQNRFDDEDGRGGRRRRNR
                     GRNRDKRRPGIGGDQVDGPVESSITEDDVLVPVAGILDILDSYAFVRTTGYLPGSSDV
                     YVPLQLVKRHGLRKGDAVTGSIKASRDGDEGQQQHVGGRNHRAKYTPLAQLDTVNGMT
                     PEDARHRPEFGKLTPLYPQDRLRLETEQGLLTTRIIDLVAPIGKGQRGLIVAPAKAGK
                     TMVMQAIANAITTNNPECHLMVVLVDERPEEVTDMQRAVKGEVISSTFDRPAEDHTTV
                     AELAIERAKRLVEMGHDVVVLLDSITKLGRAYNLAAPASGRILSGGVDSAALYPPKKF
                     FGAARNIEDGGSLTILATALVETGSRMDEVIFEEFKGTGNMELKLDRQLANRRIFPAV
                     DVNNSGTRKEEILLAPEELRIMWKLRRVLAALDSQQGIELLLDRLRKTKTNYEFLTQI
                     QHSASTKLED"
BASE COUNT          962 a         2402 c         2187 g          832 t
ORIGIN      
        1 cacgagttca tcgccaccgg tcacgaggac cagaagttcg gcttcagcct cgccggtggt
       61 caggcgttcg aggcggccac gcgcatcctc gccagccctg acgtcctcga cctgctcggc
      121 ctgcacagcc acatcggcag ccagatcttc gacaccggcg gcttcgagat ggccgcgcac
      181 cgactcgtcg cgctgcacgc ccgcatcgcc gaggaacacg gtcaccactg ccccgagatc
      241 gacctcggcg gcgggtacgg catcgcgtac acctcgcagc acacccccct cccggcgagc
      301 acgctggcag gcgagatggc cggcatcctc gaacgcgagt tcaaggccgt cggtgacggc
      361 gacctcagca cgctgccccg ggtctcgatc gagcccggtc gcgccatcgt cgggcccagc
      421 acgttcaccc tgtacgaggt gggcacggtc aagcccgtcg agctcggcgg cggccacacc
      481 cgcacctacg tctcggtcga cggcggcatg agcgacaacg cgcgcccggc gctgtacgag
      541 gccgactact cgtgcaccct ggccaatcgc cggtccgagg ccgggccgat gctgtcgcgg
      601 gtcgtcggac ggcactgcga gagcggtgac atcgtcgtgc tcgacgagta cctgccggcc
      661 gacgtgcagc ccggcgacct catcgccgtg ccgggcacgg gggcctactg ccgcgccctg
      721 tccagccagt acaaccacac tccgaggccg ccggtcgtcg ccgtccgtgg agggcggtca
      781 cgcgtgctcg tgcggcgtga gaccatcgaa gacctgctcg ccctcgacgt ggcggacgcg
      841 tgaggatcga cagcgaggag atggacccca ccatggcgca cgaaacgatg gcgcacgcca
      901 ccggtgcccc gacggaggcg cctggacgtg cccccctgcg cgtcgcgctg ctcggctgcg
      961 gcgtcgtggg ttcggcggtc gcgacgcggc tcgtcgagca cgcagacgac ctcgcggcgc
     1021 gcgtcggtgc cccgctcgag ctcgtcggca tcgccgtgcg gcggccccag cgcccgcgcc
     1081 cggacgtgcc ggtcgacccg gcgctgttca ccgcggacgc cgaggagctg gtcacccgcg
     1141 ccgacatcgt catcgaggtg atcggcggca tcgaccccgc ccgcgggctc atcctgcgcg
     1201 ccatggagca cggcgcctcg gtggtctcgg ccaacaaggc gctgctcgcc gaggacggcc
     1261 cggccctcta cgccgccgcg gatgccgccg acgtcgacct ctactacgag gccgccgtgg
     1321 ccggggccat cccgatcctg cgccccattc gtgagtccct cgccggtgac gacgtgcgca
     1381 aggtgctcgg catcgtcaac ggcacgacga acttcgtcct ggacaagatg gacaccagcg
     1441 gcgcgggttt cgccgagacc gtcgagcagg cccaggccct gggctacgcc gaggcagacc
     1501 cgaccgcgga cgtcgagggc ttcgacgccg ccgccaaggc cgccatcctc gccagcctgg
     1561 ccttccacac ccgggtctcc agcgccgacg tgcaccgcga gggcatcacc gaggtgacgg
     1621 ctggcgacat ccaggccgcc aaggagatgg actgcgtcgt caagctgctc gcgatctgcc
     1681 agcgggtgac cagcgccgac ggttcggatg ccgtgagcgt gcgcgtccac ccggcgatga
     1741 tcccgcgcag ccacccgctc gccagcgtcc gggatgcctt caacgcggtg ttcgtcgagg
     1801 ccaccgctgc cgggccgctc atgttctacg gccgcggcgc cgggggcgac ccgacggcat
     1861 ccgccgtcct cggtgacgtc gtcgccgtcg cccgccacaa ggtggccggt ggccgcggtc
     1921 cgggcgagtc cgcctacgcc gacctcccgg tggtcgacat ggggcaggcc ctcacgaggt
     1981 accacatcag cctcgacgtc gccgaccgcc cgggtgtgct cgcgcaggtc gccacggcct
     2041 tcgccgagca gggcgtgtcg atcgagaccg tgcgccagca ggtcgctgcc gggtcgggcg
     2101 agcgggcgaa gctcatcgtc gtgacgcacc gcgcatcgga tgccgcgttg tcggccaccg
     2161 tcgacgctct cgcggggctg gattccgtgg acgacgtcgc gtccgtcatg cgtgtggaag
     2221 gggactgacc agtcatggct cacctgtggc gcggagtgat ccgcgagtac gccgagcggc
     2281 tgccgatgct cgagggggca ccggtggtga ccctccagga gggcggcacg ccgctcatcc
     2341 cgtgcgaggg gctctcggag ctcaccgggg ccaacgtgtt cgtgaagttc gaagggctca
     2401 acccgacggc gtcgttcaag gaccgcggca tgacgaccgc gatgtcgatg gcgaagaagc
     2461 acggcgccaa ggccgtcatc tgcgccagca ccggcaacac gagcgccagc gccgccgcct
     2521 acgccacgaa ggccggcatg acgtgtgccg tgctcgtgcc cgacggcaag atctcgatgg
     2581 gcaagctcag ccaggccgtg gcccacggcg ccaccctgat ccaggtcgag ggcaacttcg
     2641 acgactgcct caccgtggcc cgcaagctcg ccgaggccta ccccgtcgag ctcgtcaact
     2701 cggtcaaccc cgcccgcatc gaggggcaga agaccgcggc cttcgaggtc gtcgacgccc
     2761 tcggtgacgc ccccgacatc cacatcctcc ccgtcggcaa cgccgggaac atcaccgcct
     2821 actggcgtgg ctacaccgag tacgcctcgg tgaccacggg tgtcggtggc gtcgagctgc
     2881 ccgccgtcgc gacgcgcacg ccccggatgt ggggctacca ggccgcgggg gcagcgccga
     2941 tcgtcctggg ccacccggtc gatgacccgg agacgatcgc cacggcgatt cgcatcggga
     3001 acccggcgtc ctgggcgcag gccgaggccg cccgtgacga gtccggtggg cacatcgact
     3061 ccgtgaccga cgacgagatc ctcgagggtc accggttgct gtcctcgacc gagggcatct
     3121 tcgtcgagcc cggctcggcg gcgagcatcg ccggcctgct gaaggcccac cgcgcaggca
     3181 tcgtcccgcg cgacgccacc atcgtgtgca ccgtgaccgg gcacgggctg aaggaccccc
     3241 agtgggcgct gcgcactgcc gacggtggcg acgtcacccc ggtacgtgtc ccggtcgacg
     3301 ccttcagcgt cgcggtggcc ctgggtctcg acgggtgagc accacgcggc ttcgggccgg
     3361 tgtcgaggtc gaggtgcggg tgcccgcgag cagcgcgaac ctcggccccg gtttcgactc
     3421 gatcggctgc gccctcgggg tgtgggacac ctgccgggct cgcgtcacct ccacgccggg
     3481 cctgaccgtg accgtcgccg gcgagggagc aggcagcgtt cccctcgacg cgacgcacct
     3541 cgtgcaccgg gcgatggtcg tggcctgggg tgagctcggc gtcgagccgc cgaccggcct
     3601 ggccctggac tgcacgaacg aggtgccgca cggccgggga atgggctcct cggccaccgc
     3661 gatcgtcacc ggcatcgtcg ccgcacaggc gctggcggat gtctcggccg gcgacgcggg
     3721 ggatgccgtc gacctcacct tcgcgaactc tctcgcctca cgcctggagg ggcaccccga
     3781 caacgcgtcc gcgagtgtgt acgggggctt cacgctctcc tggtccgacg acgagcaggc
     3841 cagcaccacg acggtgcacc tgccggtcca ccccgacgtc gtgccggtcg tcttcgcgcc
     3901 ggacgtccag ctgtcgaccg ccaaggcccg ctcggtgctg ccgcaacagg ttcgtctggc
     3961 ggatgccgcg gccaactccg cgcgatcagc cctgctcgcg cacgccatcg gcttcgcacc
     4021 cgaacacctg ctcgccgcca ccaaggagtg gctgcaccag gaggcccgcc gcccctcgta
     4081 tgccgcgtcg atgtccctcg tggatcggct gcggtcccgg ggccacgcgg ccgtcatctc
     4141 cggtgccggc ccgtccgtcc tggtgctggc ccggcgcgag cacgtcgagg aggtgcgcgc
     4201 gcacggtgag gccggctggc gcgtgctgac ccccggcatc cccgatcacg gggccagggt
     4261 cacggtgctg tcacgagcgt gatcgaccgg cggtgacgtc ggggcgtcgg tgctactgtg
     4321 accgtgcgac cgcagccgat gccgggcttc tctcccgatg cctgccgcga gtcgacctcc
     4381 acgatctggg aaccccgttc agcctgcgct gcggccccga cgtgaacccc ccgagatcgg
     4441 ccgcatcgag gccatccacg agcgagggaa aggatccttc gtgtcagaca ccaccactct
     4501 cgagccgtcg acggcgggcg cgagcccgcg tcgttccgga gcactcagcg ccatgcggtt
     4561 ggcagagctc caggggctgg cctcgagcat gggcatcagc ggcaccgcca agatgcgcaa
     4621 gggcgacctc gtcgccgcca tcaaggcccg ccagaacggt gagagcgggc ccgccgccac
     4681 gagcgagccg tcggcgcgcg ccagcgagcc cacgcgcggc accgaaccgc gtcgctcccg
     4741 ccgggccacg agcaccggcc gcggcgtcga gacggcacct gcgactgagc aggccacgcc
     4801 ggtcgttgcg cccgagcagg ccgcgcaggc ccccgtcgag accgcccctg cacgtgccga
     4861 cgccgagcgc gatgtccggt cggacgcagc cgagcaggcc gacggccagg cccgcgggga
     4921 gcgcggtgcc ggtcgcaccc gcacgcgcgc cgcacgccag gaccgcaccg aggcccgcga
     4981 cgcgcagtcc gaggaccgcg gcgaccagac ggcatccgcc gacaccgggt ccggtgaggc
     5041 gcagggcgag cagaacgccg gtgcccaggg cggccagaac cagaaccgct tcgacgacga
     5101 ggacggccgc ggtggccgtc gtcgccgcaa ccgtgggcgc aaccgcgaca agcgccgtcc
     5161 cggcatcggc ggcgaccagg tcgacggccc cgtcgagtcg tcgatcaccg aggacgacgt
     5221 cctcgttccc gtcgccggca tcctcgacat cctcgacagc tacgcgttcg tgcgcaccac
     5281 cgggtacctg cccggcagca gcgacgtcta cgtgccgctc cagctggtca agcgccacgg
     5341 gctgcgcaag ggcgacgccg tcaccggctc catcaaggcc tcgcgcgacg gtgacgaggg
     5401 ccagcagcag cacgtcggcg gccgcaacca ccgggcgaag tacaccccgc tggcccagct
     5461 cgacacggtc aacggcatga cgcccgagga cgcccgccac cgccccgagt tcggcaagct
     5521 cacgccgctc tacccgcagg accgactgcg cctggagacc gagcagggtc tgctgacgac
     5581 gcggatcatc gacctcgtcg cccccatcgg caagggccag cgcggcctca tcgtcgcccc
     5641 ggccaaggcg ggcaagacga tggtcatgca ggcgatcgcc aacgccatca cgacgaacaa
     5701 ccccgagtgc cacctcatgg tcgtcctcgt cgacgagcgc cccgaggagg tcaccgacat
     5761 gcagcgggcc gtgaagggcg aggtcatctc ctcgaccttc gaccgcccgg ccgaggacca
     5821 caccaccgtc gcggagctcg cgatcgagcg ggccaagcgc ctggtcgaga tggggcacga
     5881 cgtcgtcgtg ctgctcgact cgatcaccaa gctcgggcgg gcctacaacc tcgcggcccc
     5941 ggccagcggg cgcatcctgt ccggtggcgt cgactccgcc gcgctgtacc cgccgaagaa
     6001 gttcttcggc gcggcccgca acatcgagga cggtggctcg ctcaccatcc tcgcgacggc
     6061 gctggtcgag accggctcgc ggatggacga ggtgatcttc gaggagttca agggcaccgg
     6121 caacatggag ctcaagctcg accgtcagct cgcgaaccgc cggatcttcc ctgccgtcga
     6181 cgtcaacaac tcgggtactc gcaaggagga gatcctgctg gctcccgagg agctgcggat
     6241 catgtggaag ctgcgccggg tgctcgctgc gctcgactcg cagcagggca tcgagctgct
     6301 gctggaccgc ctgcgcaaga ccaagacgaa ctacgagttc ctcacccaga tccagcactc
     6361 ggcctcgacc aagctcgagg acg
//
LOCUS       DAOSOC010000143         6337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12388_length_6337_cov_10.081821, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000143 DAOSOC010000000
VERSION     DAOSOC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12388_length_6337_cov_10.081821"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..>1743
                     /locus_tag="PL014_10240"
     CDS             218..>1743
                     /locus_tag="PL014_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HOT57513.1"
                     /translation="MSSAQARTREDVLAPIAEAFEEAVPDGGGADFVRRYFRYVPLDE
                     LTSRTPDIYAGAAASHLALARDRHPGVANVRVYNPTTEVDGWSNARTVIQIVTDDMPF
                     LVDSVTGALVGAGIDIHLIVHPQLIVSRDAVGHLEAVLDRDATGKVAKGAVGEVAESW
                     MLLAVDRESDEARRNELERTVRHVLEDVRQAVEDWPKMRTKALVVAAELEGAPPEGID
                     ADETALAIRFLRWMADNHFTFLGYRDYTLRQTEAGEVIEPVTGSGLGLLRSDPPLGKA
                     PEVLSAEASAKAHEANILVLTKANSRSTVHRVAHLDYVGVKAYTDDGTVVGERRFLGL
                     YASTAYTESVLRVPLVAEKVQGVLDRSGVAHDSHTGKDLLGVLESYPRDELIQASVEQ
                     LYETTMAVTQLQERRRTKLFIREDDFGRFVSCLVYIPRDRYNTAVRMKMADLLKDTFG
                     GESVEFSARVSESALSRLQFVVRVERGKRIRRLDRAEQDLLEQRLVEVSRTWTDRLGD
                     A"
     assembly_gap    1744..1843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1844..4531
                     /locus_tag="PL014_10245"
     CDS             <1844..4531
                     /locus_tag="PL014_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HOT57514.1"
                     /translation="AAARVRDALDEVSSLDHDRIIRAFLGVILAGLRTNFYQAGDGDA
                     EHKPYVSLKLNPKAVPDLPAPRPQFEIWVYSPRVEGVHLRFGPVARGGLRWSDRREDF
                     RTEVLGLVKAQMVKNAVIVPTGSKGGFYAKDLPDPAVDREAWLEEGKAAYRLFISGLL
                     DVTDNRVSGGAIVPPERVVRHDGDDPYLVVAADKGTASFSDIANGVAQSYGFWLDDAF
                     ASGGSAGYDHKGMGITARGAWESVKRHFREMGVDTQSQDFTVVGVGDMSGDVFGNGML
                     LSEHIRLVAAFDHRHVFIDPDPVAAASFTERQRLFNLPRSSWDDYDRSLISAGGGVFP
                     RSLKSIDITPEMVAALGLPKGTSTMTPAELIHAILLAPVDLLWNGGIGTYVKAATEDH
                     LSVGDRANDAIRVDGRQVRAKVIGEGGNLGLSQLGRIEAALNGVRVNTDAIDNSAGVD
                     TSDHEVNIKILLGEVVRAGGLTTEERNELLASMTDDVAEHVLRDNYEQNVLLGNARAQ
                     ELSMASVHERLIHWLEERGDLDRGLEFLPTDAEIEKRVHDRLGLKSPEFAVLVAYAKL
                     ALKEDILASSIPDDPYFTRTLAEYFPPPIRESYADELAEHPLRREIITNSVVNSMVNR
                     GGITFAFRAQEEAGATPEQVTRAFVVCREVFGLADFVAKVEALDNVVPTTAQTGLYLE
                     FRRLLDRAVRWFLTSRPTTLDISAEIERFAGTIGDLGPRVGTLLRGDEKKRLERKVTE
                     FRKAGVPDDLAEHAASLLDWYSLLDIVDIATDTGREPDAVAPLYYLVSERFGIDTMLG
                     KVTRLPRDDRWDALARGALRDDLYSVLESLTRAVIETGGTGTEPAEQWKAWEDANKDA
                     LTRSTTALAAIRRLDNPNIAALSVALRTLRSVIRSGAAT"
     gene            4561..6048
                     /locus_tag="PL014_10250"
     CDS             4561..6048
                     /locus_tag="PL014_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HOT57515.1"
                     /translation="MSTLADMLRDTTDLEPAQSEWLHLLAGDWQLIADLSFADLVLWV
                     RVRGGLAGWRAVAHVRPNTGPMVFYDDIVGTTSSSARAAMLDEAARTHRLVAKPVTAM
                     HDDVSIREDAVPVVHAGHTIAVVTRHSNLTGARTPSRLELTYRNLADALMRMVASGEF
                     PSASAPTGMRRGAPRVGDGVIHLDPDGVVRYASPNAVSAVHRTGHVGNVVGEVLAQVV
                     ADIAARGGNTVDESLAVVAMGRAAWRSELETASATITLRAIPLTVGGVRTGAMVLLRD
                     VSELRRREQELLTKDATIREIHHRVKNNLQTVAALLRLQSRRVTDEAARAALDEAVRR
                     VGTIALVHETLSQGIDESVNFDDIAVRGLRAIVEVATREHRVDSTFTGSFGRMRAEDA
                     TALAMIISELVQNAVEHGFPDRSGTVDVAVERETLDDGEEQLTVVITDDGVGLPAGFR
                     PVLAGLGTRIVTSLASDLRGRIRWSSNEPHGTRVEFVARLRPLSR"
     gene            complement(6090..>6337)
                     /locus_tag="PL014_10255"
     CDS             complement(6090..>6337)
                     /locus_tag="PL014_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT57516.1"
                     /translation="DWRDRAACLDEDPELFFPIGNTGPAIAQIEEAKVVCRRCEVVDT
                     CLKWAIESGQDAGVWGGMSEDERRALKRRNARARRAG"
BASE COUNT          949 a         2248 c         2142 g          898 t
ORIGIN      
        1 cgttgttgcg cagctctcgg tgggcaatgg tcttcatggt ggacctcctg tagcaatcgt
       61 agcaaccaca ttcacctgcc gcgcgatctc caccctgaga caggttgggt gcggagtcgg
      121 gtcggcacgg cagcacgaag tggataggct cggcggagga cacgatggcg tgtcccaccg
      181 cagccggtga gtcgtgacca aggagtgcca ccgcaccatg tcgagcgccc aggcccgtac
      241 ccgcgaggac gtcctcgccc cgatcgctga agcgttcgag gaggcggtgc cggacggcgg
      301 aggagccgat ttcgtcaggc gctacttccg ctacgtgccc ctcgacgagc tcacgtcgcg
      361 cacccccgac atctatgccg gcgcggcggc cagccacctc gccctggcgc gcgaccggca
      421 tcccggcgtg gcgaacgtgc gggtctacaa cccgacgacc gaggtcgacg gctggtccaa
      481 cgcccgaacc gtcatccaga tcgtcaccga cgacatgccc ttcctggtcg attccgtcac
      541 cggcgccctc gtcggcgcgg gcatcgacat ccacctcatc gtccacccgc agctcatcgt
      601 gagccgcgac gccgtcggcc acctcgaggc ggtgctggac cgcgacgcca ccggcaaggt
      661 cgccaagggt gctgtcggcg aggtcgccga gtcctggatg ctgctcgccg tcgaccgcga
      721 gagcgacgag gcgcgaagga acgagctcga gcgcaccgtg cgccacgtcc tcgaggacgt
      781 tcgccaggcc gtcgaggact ggccgaagat gcgcaccaag gccctcgtcg tcgcggccga
      841 gctcgagggc gccccgcccg agggcatcga cgccgacgag acggcgctcg cgatcaggtt
      901 cctgcgctgg atggccgaca accacttcac cttcctgggt taccgcgact acaccctgcg
      961 ccagaccgag gccggcgagg tcatcgaacc cgtcaccggt tcgggcctgg gcctgctgcg
     1021 ctccgacccg ccgctgggca aggcaccgga ggtgctgagc gccgaggcgt cggccaaggc
     1081 ccacgaggcc aacatcctcg tgctgacgaa ggccaactcg cgctcgaccg tgcaccgcgt
     1141 ggcccacctc gactacgtcg gggtcaaggc ctacaccgac gacggcaccg tggtcggcga
     1201 gcgccgcttc ctcggcctct acgcatccac ggcctacacc gagtcggtgc tgcgggtgcc
     1261 gctcgtcgcc gagaaggtgc agggcgtgct cgaccgctcc ggcgtggccc acgacagcca
     1321 caccggcaag gacctcctcg gtgtcctcga gagctacccg cgcgacgagc tgatccaggc
     1381 cagcgtcgag cagctctacg agacgacgat ggccgtcacc cagctgcagg agcggcgccg
     1441 caccaagctg ttcatccgcg aggacgactt cggccgcttc gtctcgtgcc tggtctacat
     1501 cccgcgtgac cgctacaaca cggccgttcg catgaagatg gccgacctgc tcaaggacac
     1561 cttcggcggg gagtccgtcg agttctccgc gcgcgtcagc gagtcggccc tgtcccggtt
     1621 gcagttcgtc gtgcgcgtcg agcgtggcaa gcggatccgc cgtctcgacc gcgccgagca
     1681 ggacctcctc gagcagcgcc tcgtcgaggt cagccgcacg tggaccgacc gcctcggtga
     1741 cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgcgg cccgggtgcg
     1861 cgacgccctc gacgaggtgt cctcgctgga ccacgaccgg atcatccgcg cgttcctcgg
     1921 ggtgatcctg gccggcctgc gcacgaactt ctaccaggcc ggtgacggcg acgccgagca
     1981 caagccgtac gtctcgctga agctcaaccc caaggcggtg cccgacctgc cggccccgcg
     2041 cccgcagttc gagatctggg tctacagccc ccgggtcgag ggcgtgcacc tgcgcttcgg
     2101 cccggtcgcg cgcggcggac tgcgctggag cgaccggcgc gaggacttcc gcaccgaggt
     2161 cctcggcctg gtcaaggcgc agatggtcaa gaacgccgtc atcgtgccga ccggctccaa
     2221 gggcgggttc tacgccaagg acctccccga cccggccgtg gaccgcgagg cctggctcga
     2281 ggagggcaag gccgcctacc gcctgttcat ctccggcctg ctcgacgtca ccgacaaccg
     2341 ggtgtccggc ggtgcgatcg tgccgcccga gcgcgtggtg cgccacgacg gcgacgaccc
     2401 ctacctcgtc gtcgccgccg acaagggcac ggcgagcttc tccgacatcg ccaacggggt
     2461 cgcgcagtcg tacggcttct ggctcgacga cgcgttcgcc tccggtgggt cggccggcta
     2521 cgaccacaag gggatgggca tcacggcccg cggtgcgtgg gagtcggtca agcggcactt
     2581 ccgcgagatg ggcgtcgaca cccagagcca ggacttcacg gtcgtgggcg tcggtgacat
     2641 gagcggtgac gtcttcggca acggcatgct gctctccgag cacatccggc tcgtcgcggc
     2701 gttcgaccac cggcacgtgt tcatcgaccc cgatccggtg gcggccgcat cgttcaccga
     2761 gcggcagcga ctgttcaacc tgccccggtc ctcgtgggac gactacgacc ggtcgctcat
     2821 ctcggccggg ggaggggtct tcccccgctc gctgaagtcg atcgacatca cgcccgagat
     2881 ggtggcagcc ctgggcctgc ccaagggcac ctcgacgatg accccggccg agctgatcca
     2941 cgcgatcctg ctcgccccgg tcgacctgct gtggaacggc ggcatcggca cctacgtcaa
     3001 ggccgccacc gaggaccacc tctcggtcgg tgaccgggcc aacgacgcga tccgggtcga
     3061 cggccggcag gtgcgggcca aggtcatcgg cgagggcggc aacctcggcc tgtcccagct
     3121 cggccgcatc gaggcggcgc tgaacggggt tcgggtcaac accgacgcca tcgacaactc
     3181 cgccggcgtc gacacctccg accacgaggt caacatcaag atcctgctcg gtgaggtcgt
     3241 gcgcgcgggt ggtctgacca ccgaggagcg caacgagctg ctggcgtcga tgaccgatga
     3301 cgtggccgag cacgtgttgc gcgacaacta cgagcagaac gtcctgctgg gcaacgcccg
     3361 cgcgcaggag ctgtcgatgg ccagcgtcca cgaacggctc atccactggc tcgaggagcg
     3421 cggtgacctc gaccgtgggc tggagttcct gcccaccgac gccgagatcg aaaagcgggt
     3481 gcacgaccga ctgggcctga agtcgccgga gttcgccgtg ctcgtcgcct acgcgaagct
     3541 cgccctcaag gaggacatcc tcgcgagctc gatccccgac gacccgtact tcacgcgcac
     3601 cctggcggag tacttcccgc cgccgatccg ggagagctac gccgacgagc tcgccgagca
     3661 cccgctgcgc cgcgagatca tcaccaactc ggtggtcaac tccatggtca accgtggcgg
     3721 catcaccttc gccttccggg cgcaggagga ggccggagcc accccggagc aggtcacgcg
     3781 ggcgttcgtc gtgtgccggg aggtcttcgg tctggccgac ttcgtcgcca aggtcgaggc
     3841 gctcgacaac gtcgtgccga cgacggccca gacggggctc tacctcgagt tccggcgcct
     3901 gctggaccgc gcggtgcggt ggttcctcac ctcccgcccg acgaccctgg acatcagcgc
     3961 cgagatcgag cggttcgccg gcaccatcgg cgacctgggg ccccgggtcg gcacgttgct
     4021 gcgtggcgac gagaagaagc gcctcgagcg caaggtcacc gagttccgca aggctggcgt
     4081 gcccgacgac ctcgcggagc acgcggcatc cctgctcgac tggtactcgc tgctcgacat
     4141 cgtcgacatc gccaccgaca cgggtcgtga gccggatgcc gtggcaccgc tgtactacct
     4201 cgtctcggag cgcttcggca tcgacaccat gctcggcaag gtcacccgcc tgccgcgcga
     4261 cgaccggtgg gacgccctgg ctcgcggcgc gctgcgcgac gacctgtact cggtgctgga
     4321 gtcgttgacc cgtgcggtca tcgagaccgg tggcaccggc accgagccgg ccgagcagtg
     4381 gaaggcttgg gaggacgcca acaaggacgc cctgacacgc agcaccacgg cgctcgcggc
     4441 gatccgccga ctcgacaacc cgaacatcgc tgccctctcg gtggcgctgc ggacgctgcg
     4501 gtcggtcatc cgctcggggg ccgccacctg accgcggccg cgtgacctag ggttctgcgc
     4561 gtgagcaccc tggcggacat gctgcgcgac accactgacc tcgagccggc gcagtcggaa
     4621 tggctgcacc tgctcgcggg tgactggcaa ctcatcgccg acctgtcgtt cgccgacctc
     4681 gtgctgtggg tgcgggtgcg cggcggtctc gcgggctggc gggcggtggc ccacgtgcgg
     4741 cccaacaccg ggccgatggt cttctacgac gacatcgtcg gcacgacgtc gagcagtgcc
     4801 cgggcggcga tgctcgacga ggcggcgcgc acccaccggc tcgtcgccaa gccggtgacg
     4861 gcgatgcacg acgacgtctc catccgcgag gatgccgtgc ccgtcgtcca cgcgggccac
     4921 accatcgcgg tcgtcacgcg acactcgaac ctcaccgggg cgcgcacgcc cagccggctg
     4981 gaactgacgt atcgcaacct cgcggacgcc ctcatgcgga tggtggcgtc cggggagttc
     5041 ccctcggcgt cggccccgac cgggatgcgc cgtggcgcgc cccgggtcgg tgacggggtc
     5101 atccacctcg accccgacgg ggtggtgcgc tacgcgagcc ccaacgccgt gtccgccgta
     5161 caccgcaccg gacacgtggg caacgtcgtg ggtgaggtgc tggcgcaggt ggtggccgac
     5221 atcgccgccc gcggcgggaa cacggtcgac gagtccctgg cggtcgtcgc catggggcgg
     5281 gccgcctggc gctccgagct cgagaccgcg agcgcgacca tcacgctgcg ggcgatcccg
     5341 ctgacggttg gtggagtgcg caccggggcg atggtgctcc ttcgtgacgt ctccgagctg
     5401 cgccgtcggg agcaggagct gctcaccaag gacgcgacga tccgcgagat ccaccaccgg
     5461 gtgaagaaca acctgcagac ggtggccgcg ctgctgcgcc tccagtcacg gcgggtcacc
     5521 gacgaggcgg cgcgggcggc gctcgacgag gccgttcggc gggtcggcac gatcgccctg
     5581 gtgcacgaga cgctcagcca gggaatcgat gagtcggtga acttcgacga catcgccgtg
     5641 cgtgggctgc gggccatcgt ggaggtggcc acgcgcgagc accgggtcga ctcgaccttc
     5701 accggaagct tcggccggat gcgcgccgag gacgccacgg cgctcgccat gatcatctcc
     5761 gagctcgtcc agaacgccgt cgagcacggc ttccctgacc gctccggcac cgtcgacgtc
     5821 gcggtcgagc gcgagacgct cgacgacggt gaggagcagc tgaccgtggt gatcaccgat
     5881 gacggggtcg ggttgcccgc gggcttccgg ccggtgctcg ccggcctcgg cacgcgcatc
     5941 gtgacctcgc tggcctccga cctgcggggg cggattcgct ggtccagcaa cgagccgcac
     6001 ggaacgcggg tggagttcgt cgcgcggcta cgcccactca gccgctgagt cgagcagatg
     6061 tgaccacgtg gtcagacctg caccggtggt cagccggcgc ggcgcgcccg ggcgttgcgg
     6121 cgcttgaggg cgcggcgctc gtcctcggac atgccgcccc agacgccggc gtcctgcccg
     6181 gactcgatgg cccacttcag gcaggtgtcc acgacctcgc agcggcggca cacgactttg
     6241 gcctcctcga tctgagcgat cgccgggccg gtgttcccga tcgggaagaa cagctcgggg
     6301 tcctcgtcga ggcaggcagc gcggtcgcgc cagtcca
//
LOCUS       DAOSOC010000144         6298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12489_length_6298_cov_8.816595, whole genome shotgun sequence.
ACCESSION   DAOSOC010000144 DAOSOC010000000
VERSION     DAOSOC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12489_length_6298_cov_8.816595"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1335
                     /locus_tag="PL014_10260"
     CDS             160..1335
                     /locus_tag="PL014_10260"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOT57517.1"
                     /translation="MLEGLTPASLVGGLVPFVVAALTLLVFRWRRRDEVFVNLTPGEV
                     PGLGEAGESVRVRRGVEYSGAVAPRFHQPEHSTPALSGTVIDGVVHGRDLAATLVDLA
                     LRGHVLLAREGKDWRVTRADVTGDPLAPHEVALLDAVLGGATSVLLGDLRRAEVVARW
                     RAAEVAVYRAVVDRGWYTRHPRSRNGRLAALGVLLVVASAVAGVVSGLAEDHLRWAPL
                     GVGLGLGGALLLWRGRGRTPRTATGSAARIQALGYERYLATAEAGQLRHEELAGEVRA
                     MLPYAVAFGVAGHVAAVLGDALRAAQVAAGAQAALDIVVDAAIDPGVWDLVSGLVDLA
                     DLDVSGLGDLVPEDAFEALVEGVEGLGDALGHLAGAVGGILPTDGCGDGCGEGCLDF"
     gene            complement(1332..2216)
                     /locus_tag="PL014_10265"
     CDS             complement(1332..2216)
                     /locus_tag="PL014_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOT57518.1"
                     /translation="MTNRLLTLLADRPAVFDGGYGWLLQERGLPAGECAESWNVDNPD
                     AVARLHDEYAAAGAVIITTNTFGATEPRLAAHGLPGRAEEVNRAGAAIAREVADRHAV
                     LVAGDIGPTGELLEPLGTLTAQQATALFAEQVRGLAAGGADLILIETMSDLGEASAAV
                     AAAREVAPDLPVVVTMSFDTNLRTMMGVSPAAAVAALAAAGADAVGANCGRGPDEMRT
                     IAEQLVAARPEGLLLVAQSNAGLPALEGDKFVYTVGPEAMGEHAVELRDLGIDVIGAC
                     CGSSPEHIGEMAAALAGR"
     gene            complement(2260..3123)
                     /locus_tag="PL014_10270"
     CDS             complement(2260..3123)
                     /locus_tag="PL014_10270"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOT57519.1"
                     /translation="MSNELPTVALTRLLEGARYEVMPTATIADKVRAHVPLTVPLTVT
                     AAPGKGLGATFDLATALADSGYRVVPHVAARMVSGRDELVDIVARLKEHDISAIFVPA
                     GDADPPAGAYHGAVDLLRDLEEIGHSFSHVGITGYPESHPTIDDDITVQAMWDKRHYA
                     THIVSNMTFDAEHLATWIERVRRRGVTLPLLVGVPGPVERTKLLGMATKIGVGESLRF
                     LRKQKSIFARIASPGFSTDRFVSRVAALSSNPALRVEGLHVFTFNQVEVIETWRQRML
                     DEAVSARTRAR"
     gene            3204..4103
                     /locus_tag="PL014_10275"
     CDS             3204..4103
                     /locus_tag="PL014_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT57520.1"
                     /translation="MDPGTEVTTAARQGALPLTGVPTPLYAASPSKLLAFLDCPRRYR
                     LQYLDRPSPPRRAQRAHTSLGNAVHNALRDWWDLPVRTPDAGAALVDRAWIDVGFRDA
                     EQSRVWRDRMRTAVAAYLESVDPDTPPTGIERTVSFVSGDLRLTGRIDRLDDRDGELV
                     VVDYKTSRVPSTDDEARTSLALGLYAAAVWKMFRRRTLRVELHHVPSGTVASHVHTPE
                     SLTRKVEQARSIARDAQRADLDFADKGQDSEAFPPRTGPLCGWCDLRAHCPEGQAAAP
                     EKAPWAALEEPAPAGGWVARDDA"
     gene            4093..4647
                     /locus_tag="PL014_10280"
     CDS             4093..4647
                     /locus_tag="PL014_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT57521.1"
                     /translation="MTRDRHPSAFGTQRLLLRQWRESDLEPFARLNADPEVMRWFPAP
                     LSRDQSDAMVDRLRGHIEAEGWGMWAVERRDTGEFIGFTGLAVPRQELPFQPCVEVGW
                     RLARDAWGHGYASEAAREALRVGFDELGLTEIVAFTAVANSRSRAVMERLGMVRDAAE
                     DFDHPAVGVGHPVRRHVLYRKVRP"
     gene            complement(4637..4870)
                     /locus_tag="PL014_10285"
     CDS             complement(4637..4870)
                     /locus_tag="PL014_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57522.1"
                     /translation="MNTSTLWTTVKDELRERREARAAEAALRADLANYRTPSDIEDLL
                     ASVDSHDTPEAEMIRDVLMDNLRTYHQRRAFTA"
     gene            4946..5863
                     /locus_tag="PL014_10290"
     CDS             4946..5863
                     /locus_tag="PL014_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT57523.1"
                     /translation="MDTDALRWFQLVADGYTVTEVSEVFGVSQPGVSRALARLEKEVG
                     TPLLRRSGRVLRMTHAGVAFKRHVDSLVNDLDDGLAAVSELVDPEAGVVTLAFPLSLG
                     SWLVPRLLTGFRRGHPRVKFVLLRTAVGESGRVSQHLATRSADVELTTHRVHGPDIEW
                     RRVLVEPLVLAVGLAHPLASRDEVSLSEVASEPFIMRRAPSGMRGQTLDLCHAAGFEP
                     EVELEADDLLTVRGLVAAGLGVAVVPAMGLPAPTTFARSRLLPIVDAGAQREVGVAWV
                     RGRPLLPSGEAFRHFVLADGAMDGHAHHE"
     gene            5907..>6298
                     /locus_tag="PL014_10295"
     CDS             5907..>6298
                     /locus_tag="PL014_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT57524.1"
                     /translation="MAPKRKATIIALANQKGGVAKTTSVASLGAAFAEQGRRVLLVDL
                     DPQACLTFSLGVDPDTVESSVHDVLVGGVEVADVIVHCEEGIHLVPSTIDLAGAEAVL
                     LSRPAREYVLQSALDAVRRDYDIILLDCS"
BASE COUNT          798 a         2237 c         2314 g          949 t
ORIGIN      
        1 gctgcgcacc ctggcgcagg ccgcggccga ccgcatcggg ctgccgctcc aggtgaggca
       61 caccggtgag gacggcctcg agcgcgccct cgcctccctc cttcccgacc tctcctgacc
      121 gacctgcaca tcaggggcgg gtcgtctagg gtcgtgcagg tgctcgaggg actgactcct
      181 gcgagcctcg tcgggggact ggtgcccttc gtcgtggccg cgctgactct gctcgtcttc
      241 cggtggcggc ggcgtgacga ggtgttcgtc aacctcacgc ccggcgaagt cccggggctc
      301 ggtgaagccg gtgagagcgt ccgagtgcgc cgcggcgtgg agtactcggg cgccgtggct
      361 ccccgcttcc accagcccga gcactcgacc ccggccctgt cgggcacggt gatcgacggc
      421 gtggtgcacg ggcgtgacct cgccgccacc ctcgtggacc tggccctgcg cgggcacgtg
      481 ctcctcgcgc gcgagggcaa ggactggcgg gtcaccaggg ccgacgtgac gggagacccg
      541 ctcgcccccc acgaggtggc gcttctcgac gccgtgctcg gtggcgcgac ctcggtgctg
      601 ctcggcgacc tgcgacgggc cgaggtcgtg gcgcgctggc gggccgccga ggtggcggtc
      661 taccgggctg tggtcgaccg gggctggtac acccggcatc cgcgctcgcg caacgggcgg
      721 ctcgctgcgc tgggggtgct cctcgtggtg gcatcggccg tggcaggcgt ggtctccgga
      781 ctggcggagg accacctgcg atgggcccct ctgggagtgg ggctcggcct cggcggcgcc
      841 ttgctgctgt ggcgcgggcg cggacgcact ccgcgcacgg ccacggggtc cgcagcgcga
      901 atccaggccc tgggctacga gcgctacctc gccaccgctg aggccggcca gctgcggcac
      961 gaggagctcg ccggcgaggt gcgcgccatg ttgccctacg ccgttgcctt cggggtcgcg
     1021 ggccacgtcg ccgccgtcct gggtgatgcc ctgcgtgccg cccaggtcgc ggcgggcgcc
     1081 caggccgcgc tcgacatcgt cgtcgacgcc gccatcgacc ccggcgtgtg ggacctcgtg
     1141 tcggggctgg tcgacctcgc cgacctggac gtgagcgggc tcggcgacct cgtgccggag
     1201 gacgccttcg aggccctggt cgagggggtc gaggggctcg gcgacgccct gggccacctg
     1261 gccggcgccg tcggtggcat cctgcccacc gacggctgcg gcgacggctg cggcgagggg
     1321 tgcctggact tctagcggcc cgccagggcg gcggccatct cgccgatgtg ctcgggtgag
     1381 gatccgcagc acgctccgat gacgtcgatg ccgaggtcgc gcagttcgac cgcgtgctcc
     1441 cccatggcct cggggccgac ggtgtacacg aacttgtcac cctcgagcgc gggcagtccg
     1501 gcgttcgact gggccacgag gagcagcccc tcggggcggg ccgccacgag ctgctcggcg
     1561 atggtgcgca tctcgtcggg cccgcggccg cagttggccc cgacggcgtc ggcgcccgcg
     1621 gccgccagcg ccgccacggc ggcggccggt gagacgccca tcatggtgcg caggttcgtg
     1681 tcgaaggaca tcgtcacgac gaccggcagg tcgggtgcga cctcgcgcgc ggccgccacg
     1741 gcggcgctcg cctcgccgag gtcactcatc gtctcgatca ggatgaggtc ggcgccaccg
     1801 gcggccagcc cccgcacctg ctcggcgaac agcgcggtcg cctgctgcgc ggtcagggtt
     1861 ccgagcggct cgagcagctc acccgtcggg ccgatgtcgc cggccacgag caccgcgtgc
     1921 cggtctgcga cctcccgcgc gatggcagcc cccgcccggt tgacctcctc cgcccggccg
     1981 ggcaggccgt gggcggcgag acggggctcg gtggcgccga aggtgttggt ggtgatgatg
     2041 acggcacccg cggcggcgta ctcgtcgtga agcctcgcca cggcatccgg gttgtcgacg
     2101 ttccacgact ccgcgcactc gcccgcaggg agcccacgct cctgcagcaa ccagccgtat
     2161 ccgccgtcga agacggccgg gcggtcagcg agcagggtca gcagtcgatt cgtcaccaag
     2221 gaagggtacg cagcgcggat gccgtgcccc gccgccgtct catcgggcgc gggttcgggc
     2281 gctcaccgct tcgtcgagca tgcgctggcg ccaggtctcg atgacctcga cctggttgaa
     2341 ggtgaagacg tgcagcccct cgacccgcag ggccggattg ctcgacaggg cggcgacccg
     2401 gctgacgaag cggtcggtgg agaacccggg cgacgcgatg cgggcgaaga tcgacttctg
     2461 cttgcgcagg aaccgcagtg actcacccac tccgatcttc gtcgccatgc cgagcagctt
     2521 cgtgcgctcg acgggcccgg ggacaccgac gagcaggggg agggtgaccc cgcggcggcg
     2581 cacgcgttcg atccaggttg cgaggtgctc ggcgtcgaag gtcatgttcg acacgatgtg
     2641 ggtggcgtag tgccgcttgt cccacatggc ctggaccgtg atgtcgtcgt cgatggtcgg
     2701 gtgcgactcg gggtagccgg tgatcccgac gtggctgaag ctgtggccga tctcctcgag
     2761 gtcgcgcagc aggtcgacgg cgccgtggta cgccccggcc ggcgggtcgg cgtcaccagc
     2821 aggcacgaag atcgccgaga tgtcgtgctc cttcaggcgg gcgacgatgt cgacgagttc
     2881 gtcccggccc gagaccatgc gtgctgccac gtgcggcacc acccggtagc cgctgtcggc
     2941 gagcgccgtg gcgaggtcga acgtggcacc gaggcccttg cccggtgccg ccgtgacggt
     3001 gagcggcacg gtgaggggca cgtgggcccg caccttgtcc gcgatcgtcg cggtgggcat
     3061 cacctcgtag cgggcaccct cgaggaggcg ggtcagtgcc accgttggca attcgttgct
     3121 catcatgcaa cctgtctcgt catacgcacc gaatgtcgct tcagcgtaga gggcgtcggt
     3181 gctcggcgct agcgttgccg accatggatc cggggaccga ggtcacgaca gcggcacgcc
     3241 agggcgccct gccgctcacg ggtgtgccca cccccctcta tgccgccagt ccgagcaagc
     3301 tcctggcgtt cctggactgc ccccgccgct accggctgca gtacctcgac cggccctccc
     3361 cgccccgtcg ggcgcaacga gcccacacct cgctcggcaa cgccgtccac aacgcgctgc
     3421 gcgactggtg ggacctcccg gtcaggacgc cggatgccgg ggcggccctc gtcgaccgcg
     3481 cctggatcga cgtcgggttt cgtgacgccg agcagtcccg cgtctggcgc gatcggatgc
     3541 gcaccgcggt ggcggcctac ctggagtccg tcgacccgga caccccgccg accgggatcg
     3601 agcgcacggt gtccttcgtc agcggcgacc tgcgcctgac cgggcgcatc gaccggctcg
     3661 acgaccgcga tggtgagctc gtcgtcgtcg actacaagac ctcacgggtg ccgtccaccg
     3721 acgacgaggc gcgcacctcg ctggcgctgg gcctctacgc cgccgccgtg tggaagatgt
     3781 tccgacggcg cacgctgcgc gtcgagctgc accacgtgcc ctccggcacg gtggcctcgc
     3841 acgtccacac gcccgagtcg ctgacccgca aggtcgagca ggcgcgctcg atcgcccgtg
     3901 acgcccagcg ggccgacctc gacttcgccg acaagggtca ggacagcgag gcgttcccgc
     3961 cccggaccgg cccgctctgc gggtggtgtg acctgcgggc gcactgcccc gagggtcagg
     4021 cggccgcccc cgagaaggcg ccctgggccg cgctcgagga gccggccccg gctggtgggt
     4081 gggtggcgcg cgatgacgcg tgaccggcat ccgtcggcgt ttggcacgca gcgattgctc
     4141 ctgcggcagt ggcgggagtc ggacctcgaa ccgttcgccc ggctcaacgc cgaccccgag
     4201 gtcatgcggt ggttcccggc gccgctcagc cgcgatcagt ccgacgccat ggtcgaccgg
     4261 ctgcgcggcc acatcgaggc cgaagggtgg gggatgtggg cggtcgaacg ccgcgacacg
     4321 ggggagttca tcgggttcac cggcttggcg gtgccccggc aggagctgcc gttccagccc
     4381 tgcgtggagg tgggctggcg cctggcccgc gatgcatggg gccatgggta cgcgagcgag
     4441 gcggcacgcg aggcgctgcg ggtggggttc gacgagctcg gcctcaccga gatcgtcgcg
     4501 ttcacggccg tggcgaactc gcggtcacgg gcggtgatgg agcggttggg gatggtgcgc
     4561 gacgcggccg aggacttcga ccaccccgcg gtgggcgtgg ggcacccggt gcgccgccac
     4621 gtcctctatc gaaaggtcag gccgtgaacg cgcgacgctg gtggtaggtg cgcaggttgt
     4681 ccatgagcac gtcgcggatc atctcggcct cgggggtgtc gtgcgagtcc acggaggcga
     4741 ggaggtcctc gatgtcggag ggggtgcggt agttggccag gtcggcgcgc agggcggcct
     4801 cggcggcgcg ggcctcgcgg cgctcgcgaa gctcgtcctt gacggtggtc cacagggttg
     4861 aggtgttcat gtatccagtg tcgccaccga caagcaatgc gtccaatgaa atccagacat
     4921 acgaaccatg cgatatgatc atgtaatgga cactgacgcc ctgcgatggt tccagctcgt
     4981 ggccgacggt tacaccgtga ccgaggtcag cgaggtcttc ggcgtgagcc aacccggggt
     5041 ttcccgagca ctggcccgcc tcgagaagga ggttggcacg ccgctgctgc gccgatccgg
     5101 ccgggtgctg cggatgaccc acgccggggt ggccttcaag cggcacgtcg actccctggt
     5161 caatgacctc gacgacgggc tcgccgcggt cagcgagctc gtcgaccccg aggccggggt
     5221 ggtgacgctc gcgttcccgc tcagcctggg gtcctggctc gtcccgcgcc tcctcaccgg
     5281 ctttcgccgc gggcacccgc gggtgaagtt cgtgctgttg cgcacggccg tcggtgagag
     5341 cggccgggtc tcccagcacc tcgcgacccg ctcagccgat gtcgagctca ccacgcaccg
     5401 cgtccacgga cccgacatcg agtggcggcg cgtgctcgtc gagcccttgg tccttgccgt
     5461 cggactcgcc cacccgctcg cgtcccgcga cgaggtgtcg ctgagcgagg tcgcctccga
     5521 gccgttcatc atgcggcggg ccccctcggg catgcggggc cagaccctcg acctctgcca
     5581 cgccgctggg ttcgagcccg aggtcgagct cgaggccgac gacctgctca ccgtgcgcgg
     5641 cctcgtcgcg gccggtctgg gcgtggccgt cgtgccggcg atggggttgc cggctcccac
     5701 gacgttcgcc cgcagccgcc tgctgcccat cgtggatgcc ggggcgcagc gtgaggtggg
     5761 tgtggcctgg gttcgtggtc gccctctcct gccctcgggg gaggccttcc ggcacttcgt
     5821 gctcgccgac ggggcaatgg acggccacgc ccaccacgag tgacacaatc ggggccactg
     5881 cccgcggacc ccggaaaggc ttcccgatgg cgcccaagcg caaggccacg atcatcgccc
     5941 tggcgaacca gaagggcgga gtggccaaga ccacgtccgt cgcctcgctc ggggcggcgt
     6001 tcgccgagca gggtcggcgg gtcctcctcg tcgacctcga cccccaggcc tgcctgacct
     6061 tcagcctcgg tgtcgacccc gacaccgtcg agtcgtctgt gcacgacgtg ctcgtcggcg
     6121 gagtcgaggt ggccgacgtc atcgtgcact gcgaggaggg catccacctc gtgccgagca
     6181 cgatcgacct cgcgggagcc gaagccgtgc tgctctcgcg cccggcccgc gagtacgtgc
     6241 tgcagtccgc gctggatgcc gtgcggcgcg actacgacat catcctgctc gactgctc
//
LOCUS       DAOSOC010000145         6235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12651_length_6235_cov_9.403722, whole genome shotgun sequence.
ACCESSION   DAOSOC010000145 DAOSOC010000000
VERSION     DAOSOC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12651_length_6235_cov_9.403722"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="hemW"
                     /locus_tag="PL014_10300"
     CDS             complement(<1..890)
                     /gene="hemW"
                     /locus_tag="PL014_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOT57525.1"
                     /translation="MPRLPDGDPAPESGDLPEPALATLGDGRFGVYVHVPYCSVRCGY
                     CDFNTYTLGELGPGASMATFADQALAELDLAARVLGDQARPVDTVFVGGGTPTLLPAA
                     DLVRCVDGIRTRFGLSPDAEVTTEANPDSVTADDLAELAAGGFTRVSIGMQSVVPHVL
                     RTLERTHDPARVATAVAAAKAAGLATSVDLIYGTPGESLADWRASLDAAVALEPDHVS
                     AYALIVEDGTKLAAQVRRGVVAMPDGDDEADKYELADDVLAAAGYDWYEVSNWSRTPA
                     GRCRHNEGYWRGDAWWGVGPG"
     gene            946..2160
                     /locus_tag="PL014_10305"
     CDS             946..2160
                     /locus_tag="PL014_10305"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HOT57526.1"
                     /translation="MSTDVLVVGGGPAGRALAGACARAGLSTALADPQLDRPFTATYG
                     CWADELPAGIPESTIAARARGRAIAVGTHDLGWEYAVFDVEALRAHLDAQLAGVEVHA
                     GRVSGPAGPGRVALEGGGTLTASVVVDAAGRWQPLNGTARRRPGAGAPVAAEQTAFGV
                     VVPEEAAAALVPPGEALFMDWRADHGEPGWPTFLYGIPVGRGEVLLEETSLARRPGLP
                     VPALRRRLLARLAHHRIAVPDGARTETVRFPVDRPRHDVDGVLGFGAAAPLVHPATGF
                     SIAAALGLAGPVADAVAAHLPDGPDAALAAARAVVWSPSARAVHRFRRIGLEALLRMP
                     PAEVPGFFDVFFALPSHQRWAYLTGRDDLGGTMRTMGGLFAAADLRLRRRLVLPALRR
                     PLPSNDGVGAPT"
     gene            2170..2952
                     /locus_tag="PL014_10310"
     CDS             2170..2952
                     /locus_tag="PL014_10310"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOT57527.1"
                     /translation="MTDRWIELDGLVNMRDLGGLPTRDGGRTASGRLIRSDNLQDLTD
                     ADVAHLVHAVGVSDIIDLRTEVEVHLEGPGPLRRVESLTHHHHSLIAARGEEDLDALA
                     AKALAVPWSPEGTRRDASFWAEHYLGYLAARPDSVAAALRVIGDAPGAAVVHCAAGKD
                     RTGTVVAMALDVAGVPHEEIVADYVLTAERIEAIIGRLMSRPAYGHALSQQSIDEQSP
                     KPESIEAILRAVDAGWGGAAGWLRDHGWTASDVEGLRHHLTR"
     gene            complement(2942..4573)
                     /locus_tag="PL014_10315"
     CDS             complement(2942..4573)
                     /locus_tag="PL014_10315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT57528.1"
                     /translation="MCPSAQPADLITVLHGRVWCPVQGDRDAVAIRGDLVIAVDAAAL
                     ALADTPGAAVVEVGDGILIPAFGDGHAHPVFAGLQAHGPHISDAGSVAEVVAQVARWA
                     AEHPHEPWIRGASYDPALAPDGLFDARWLDEAVPDRPVVLRAADYHTVWVNTTALHLA
                     GIDASTPAPPLGEIARRADGTPLGTLREWGAVDLVLDVAGPHPLDVRVRALEEATTHL
                     ASLGITWVQDAWVEADDVEAYLAASAQKRLHTAVDLALYADPRRGPQQVERLVALRDR
                     VRERGDPLLTANTVKLFADGVIEGGTAALLDPYAGSGCAHRGMLVWEPVALVAIVTAV
                     DAHGFQVHVHTIGDAAVRTALDAVEAAISANGPRPDRRPVLAHVQLIDEADRPRLAAL
                     GVIANAEPLWAQLDDVMEALTVPRLGEPRASAQYPWATLRDLGTRLSFGSDWPVSSPD
                     PLAGIATACTRQTEDRMPAAGWTPHERLSLTAALTAYTAGSAYQAGRRAGTLEVGNDA
                     DLVLLDRDPFALTDPGDLANARVTRTWLRGRSLSG"
     gene            complement(4548..5012)
                     /locus_tag="PL014_10320"
     CDS             complement(4548..5012)
                     /locus_tag="PL014_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HOT57529.1"
                     /translation="MPDTYALYRSMSARPFGKRAFSLLFANKAPYFRTVRPQVRDVRP
                     HHAELTIRNRRAVHNHIGTLHAIAVCNGLEAAMGLLAEATTPKDRRWIPKGMQVSYLA
                     KSTTDLLCIAETDPADWAQLGDVPVRVRAVRTDGTVVVDGIITIYVSERPAR"
     gene            5061..5636
                     /locus_tag="PL014_10325"
     CDS             5061..5636
                     /locus_tag="PL014_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT57530.1"
                     /translation="MTTPSYPGQPGFVVPAPEDRPRFDRRAVRVLVLDEDDRMLLFKD
                     SDLGLDPVAHWWVTPGGGVDPGESDLEAAVRELWEETGLVVDAGDLVGPLLTRVVVHG
                     YSDKVVDQHEVFYVVRVAGFEVDTTSHTEEEQLTVADIRWWALPDLAATDDDVWPRDV
                     LAVLELASRPHEWADGPVVAPPVQESSVPVP"
     gene            5675..>6235
                     /locus_tag="PL014_10330"
     CDS             5675..>6235
                     /locus_tag="PL014_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOT57531.1"
                     /translation="MDVKSTVWHYLKNAREQVLWKVEGLPERDQRMPLTPTGTNLLGV
                     VKHLAGVEAEYFGMCLGRPWPQPMAFWADDAQPNDDMWATADESPAQILDLYRQVTAF
                     ADETIAPLPLDTRAHVPWWREPDTTLHHLLVHMTAETARHAGHLDILREQVDGQAGWS
                     PEHPNLPEGGADHWAAYVARLREIAER"
BASE COUNT          791 a         2271 c         2364 g          809 t
ORIGIN      
        1 cccggcccga cgccccacca ggcgtcaccg cgccagtacc cctcgttgtg ccggcagcgc
       61 cccgccggcg tgcgcgacca gttgctgacc tcgtaccagt cgtagcccgc cgcggcgagg
      121 acgtcgtcgg cgagctcgta cttgtcggcc tcgtcgtcac cgtcgggcat cgcgaccact
      181 ccccgacgca cctgcgccgc gagcttcgtc ccgtcctcga cgatcagcgc gtaggccgag
      241 acgtggtcgg gctccagggc gacggcggcg tcgaggctgg cgcgccagtc ggcgaggctc
      301 tcgcccgggg tgccgtagat gaggtcgacg ctcgtcgcca gcccggcagc cttggccgca
      361 gcgaccgcag tcgccacgcg ggcggggtcg tgggtgcgct cgagggtgcg caacacgtgc
      421 ggcaccaccg actgcatgcc gatcgagacc cgggtgaacc cacccgcggc gagctcggcg
      481 aggtcgtcgg cggtcaccga gtcagggttg gcctcggtgg tcacctcggc gtccggggac
      541 aggccgaaac gcgtccggat gccgtcgacg caccgcacga ggtcggcggc gggaagcagg
      601 gtgggcgtgc ctcccccgac gaagaccgtg tcgaccggcc gagcctggtc gccgagcacc
      661 cgagcggcga ggtcgagctc ggccagggcc tggtcggcga aggtcgccat cgacgccccc
      721 ggcccgagct caccgagggt gtaggtgttg aagtcgcagt agccgcaccg caccgagcag
      781 tagggcacgt gcacgtagac gccgaaccgg ccgtccccga gcgtggccag ggccggctcg
      841 ggcaggtcac cggactcggg cgcggggtca ccgtcgggca gtcggggcac gtccttatcc
      901 tcgcccacgc accgcagccg tggtggcgcc ggttagggtc ggcgcgtgag cacggacgtc
      961 ctcgtcgtcg gcggcggccc cgcgggccgg gcgctcgccg gggcgtgcgc gcgggccggc
     1021 ctgagcaccg cgctcgccga cccgcagctc gaccgcccct tcacggcgac gtacgggtgc
     1081 tgggcggacg agctgcccgc cggcatcccc gagtcgacga tcgccgcccg ggcgcggggt
     1141 cgcgccatcg cggtcggcac ccacgacctc ggctgggagt acgccgtgtt cgacgtcgag
     1201 gccctgcggg cccacctgga cgcgcagctg gccggggtcg aggtgcacgc cggcagggtc
     1261 agcgggccgg ccggccccgg acgggtcgcg ctcgagggcg ggggcacgct cacggcatcc
     1321 gtcgtcgtcg acgcggcggg gcggtggcag cccctgaacg gcacggcccg gcggcggccc
     1381 ggtgccggcg cgccggtcgc ggccgagcag accgccttcg gcgtcgtcgt gcccgaggag
     1441 gcggccgcgg cgctggtgcc ccccggcgag gcgctgttca tggactggcg cgcggaccac
     1501 ggcgagccgg gctggcccac gttcctctac ggcatccccg tcgggcgtgg tgaggtgctg
     1561 ctcgaggaga cctcgctggc ccgccgtccg gggctccccg tccccgcgct gcggcgccgg
     1621 ctgctggccc ggctcgcgca ccaccgcatc gcggtgccgg acggggcccg caccgagacc
     1681 gtgcgcttcc ccgtcgaccg gccacgccac gacgtcgacg gggtgctcgg cttcggcgcg
     1741 gcggcgccgc tcgtgcaccc cgcgaccggc ttcagcatcg ccgccgccct cgggctcgcc
     1801 ggaccggtcg cggatgccgt ggcggcgcac ctccccgacg gccccgacgc cgcgctcgcg
     1861 gccgcccgcg ccgtcgtgtg gtcgccgtcg gcccgggcgg tgcaccggtt ccggcgcatc
     1921 gggctcgagg cgctgctgcg gatgccgccc gccgaggtcc ccggcttctt cgacgtcttc
     1981 ttcgccctcc cgtcgcacca gcgctgggcc tacctgaccg gccgcgacga cctcgggggc
     2041 accatgcgca cgatgggcgg cctgttcgcc gccgccgacc tgcggcttcg ccgccggctc
     2101 gtgctgccgg ccctgcgtcg acccctgccc agcaacgacg gcgtcggtgc cccgacctag
     2161 gatgaccggg tgactgaccg gtggatcgag ctcgacgggc tcgtcaacat gcgcgacctc
     2221 ggagggttgc cgacccgcga cggcgggcgg acggcatccg ggcggttgat ccgttccgac
     2281 aacctccagg acctcaccga cgccgacgtc gcacacctcg tccacgcggt cggggtgagc
     2341 gacatcatcg acctgcgcac cgaggtcgag gtgcacctcg agggccccgg gccgctgcgc
     2401 cgggtggagt cgctgaccca tcaccaccac tcactcatcg ccgcccgcgg cgaggaggac
     2461 ctcgacgcgt tggcggccaa ggccctggcg gttccgtgga gccctgaggg cacccgccgc
     2521 gacgcgtcgt tctgggccga gcactacctg ggctacctcg cggcccgacc cgactcggtc
     2581 gcggctgcac tgcgggtgat cggcgatgcg ccgggcgcgg cggtggtgca ctgcgccgcg
     2641 ggcaaggacc gcaccggcac ggtcgtcgcg atggcactcg acgtggcggg ggtcccgcac
     2701 gaggagatcg tggccgacta cgtgctcacg gccgagcgga tcgaggccat catcggccgc
     2761 ctgatgtcac gcccggcgta cgggcatgcc ctgtcgcagc agtcgatcga cgagcagtca
     2821 cccaagcccg agtcgatcga ggcgatcctg cgtgccgtcg acgccggctg gggcggagcg
     2881 gccgggtggc tgcgcgacca cgggtggacc gcctccgacg tcgagggact gcgccaccac
     2941 ctcacccgct gaggctcctc ccgcggagcc aggtccgcgt gacccgcgcg ttggcgaggt
     3001 cgccggggtc ggtgagggcg aaggggtcgc ggtcgagcag cacgaggtcg gcgtcgttcc
     3061 cgacctcgag ggtgcccgct cgacgtccgg cctggtaggc actccccgcg gtgtaggccg
     3121 tgagcgccgc agtcagcgac agccgctcgt gcggggtcca gcccgcggcc ggcatccggt
     3181 cctcggtctg ccgggtgcag gcggtcgcga tgccggcgag cgggtcgggc gagctgaccg
     3241 gccagtccga gccgaagctg agcctggtgc cgaggtcgcg cagcgtcgcc cacgggtact
     3301 gcgccgaggc gcggggctcc cccaaccgcg gcacggtcag ggcctccatg acgtcgtcga
     3361 gctgcgccca gagcggctcg gcgttggcga tgaccccgag ggccgccagc cgggggcggt
     3421 cggcctcgtc gatgagctgc acgtgcgcga gcaccggccg gcggtccggc cgggggccgt
     3481 tggcgctgat cgcggcctcg acggcatcga gcgccgtgcg caccgcggcg tcgccgatgg
     3541 tgtgcacgtg aacctggaag ccgtgcgcgt cgacagcggt gacgatggct accagggcca
     3601 cgggctccca gacgagcatg ccgcggtggg cgcacccgct gccggcatac gggtcgagca
     3661 gcgccgcggt gccgccttcg atgacaccgt cggcgaacag cttcacggtg ttggcggtca
     3721 gcagcgggtc gccgcgctcg cgcacccggt cgcgcagggc gacgagccgc tcgacctgct
     3781 gcggcccgcg gcgcgggtcg gcgtacaggg cgaggtcgac ggcggtgtgc agcctcttct
     3841 gtgcggatgc ggcgaggtag gcctcgacgt cgtcggcctc gacccaggcg tcctgcaccc
     3901 aggtgatccc gagcgaggcc aggtgggtgg tggcttcctc gagcgcgcgc acccggacgt
     3961 cgagggggtg cgggcccgcg acgtcgagca cgaggtcgac cgcgccccac tcgcgcagcg
     4021 tgccgagcgg cgttccgtcg gcgcgtcggg cgatctcacc gagcggcggg gcgggcgtcg
     4081 aggcgtcgat gccggcgagg tgcagcgccg tcgtgttgac ccacaccgtg tggtagtcgg
     4141 cggctcgcag caccacgggt cggtcgggca cggcctcgtc gagccaccgg gcgtcgaaca
     4201 gcccgtcggg cgcgagcgcc gggtcgtagg acgccccgcg gatccacggc tcgtgcgggt
     4261 gctcggccgc ccagcgcgcg acctgcgcga cgacctcggc gaccgacccg gcatccgaga
     4321 tgtgtggtcc atgggcctgc aggcccgcga acaccgggtg ggcgtgcccg tcaccgaacg
     4381 ccgggatgag gatgccgtca cccacctcga ccacagcggc accgggcgtg tccgcgaggg
     4441 cgagcgcagc ggcatccacg gcaatgacga ggtcaccgcg gatcgcgacc gcgtcgcggt
     4501 ccccctgcac cgggcaccag acgcgcccgt gaagaaccgt gatcaggtca gcgggctggg
     4561 cgctcggaca catagatcgt gatgatgcca tcgacgacga cggtgccatc ggtacgaacc
     4621 gccctgaccc gcaccggcac gtcgccgagc tgcgcccagt cggccgggtc ggtctcggcg
     4681 atgcacagca ggtcggtggt cgacttcgcg aggtaggaga cctgcatccc cttggggatc
     4741 cagcgccggt ccttcggcgt ggtggcctcg gcgagcaggc ccatcgctgc ctcgagcccg
     4801 ttgcacaccg cgatggcgtg cagggtgccg atgtggttgt gcaccgcccg gcggttgcgg
     4861 atggtcagct cggcgtggtg cggtcgcacg tcgcgcacct gcggccgcac ggtgcggaag
     4921 tagggggcct tgttcgcgaa gagcagcgag aaggcgcgct tgccgaacgg gcgggcggac
     4981 atcgagcggt acagcgcgta ggtgtcaggc acccggccga ggttaccggc gagtactgcc
     5041 ggccgggtta acgtgtggcc atgaccaccc cctcctaccc cggccagccc ggcttcgtcg
     5101 tgcccgcacc cgaggaccgg ccacggttcg accggcgggc ggtgcgggtg ctggtgctcg
     5161 acgaggacga ccggatgctc ctgttcaagg actccgacct cgggctggac ccggtcgcgc
     5221 actggtgggt gacgcccggc gggggtgtcg acccgggcga gagcgacctc gaggccgcgg
     5281 tgcgcgagct gtgggaggag accgggctcg tcgtggatgc cggggacctc gtcggcccgc
     5341 tgctcacccg ggtcgtcgtg cacggctaca gcgacaaggt ggtcgaccag cacgaggtgt
     5401 tctacgtggt gcgggtggcg ggcttcgagg tggacaccac gtcgcacacc gaggaggagc
     5461 agctgaccgt ggccgacatc cggtggtggg ccctgcccga cctcgccgcc accgacgacg
     5521 acgtctggcc ccgcgacgtg ctggcggtgc tcgagctggc ctcccgaccg catgagtggg
     5581 ccgacggccc ggtggtcgcc ccgcccgtgc aggagtcgag cgtccccgtc ccgtgacggg
     5641 gagctgtgac agtgcctcgt gacaggctgc cgtcatggac gtcaagagca cggtatggca
     5701 ctacctgaag aacgctcgcg agcaggttct ctggaaggtc gaggggctgc ccgagcgcga
     5761 ccagcggatg ccgctcaccc ccaccggcac gaacctgctc ggtgtcgtca agcacctggc
     5821 cggggtcgag gccgagtact tcggcatgtg cctgggccgc ccgtggccgc agccgatggc
     5881 gttctgggcg gacgacgcgc agcccaacga cgacatgtgg gctaccgcag acgagtcgcc
     5941 ggcgcagatc ctcgacctct accgccaggt gacggcgttc gccgacgaga ccattgcgcc
     6001 cctgcccctg gacaccaggg cccacgtgcc gtggtggagg gagccggaca ccacgctgca
     6061 ccacctgctg gtgcacatga ccgctgagac ggcccggcac gccgggcacc tggacatcct
     6121 gcgcgagcag gtcgacggtc aggccggctg gtcgcccgag cacccgaacc tgcccgaggg
     6181 cggcgcggac cactgggcgg cctacgtggc ccggctccgc gagatcgccg agcgc
//
LOCUS       DAOSOC010000146         6230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12662_length_6230_cov_7.737328, whole genome shotgun sequence.
ACCESSION   DAOSOC010000146 DAOSOC010000000
VERSION     DAOSOC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12662_length_6230_cov_7.737328"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PL014_10335"
     CDS             <1..385
                     /locus_tag="PL014_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT57532.1"
                     /translation="VIQNVLPSVLDATYAQIAAAAPDAHVVVTGYPRLFSPESGDYFN
                     ASPAEQQAMNDGADLLNSVIAEAAGDAGFQFVDVTSRFVGHGVNAPEPWVLGVTDPGR
                     FHPNLAGYEAYAAAVTSQVNPRDLR"
     gene            complement(410..964)
                     /locus_tag="PL014_10340"
     CDS             complement(410..964)
                     /locus_tag="PL014_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOT57533.1"
                     /translation="MTASTRERALEAAVAVLGAEGVRALTHARVDARAGLPPGSTSNW
                     FRTRRALLAGVVDWIAERERADFDPGAMPVVTDAEELVRGLCAMAELQTGPFAARTRA
                     RYALFLELAGDPGLGESLRRQRREFEAWTEHLVGTLGFADPVPATRALMATLDGLLLH
                     RLTVDPGLDLRPAIDRVVRSLAAT"
     gene            1030..1935
                     /locus_tag="PL014_10345"
     CDS             1030..1935
                     /locus_tag="PL014_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT57534.1"
                     /translation="MPEPTAPWSPPLPAAPGFEHLVVETPGLRSHVAVIGDGDGDGDG
                     GGDPVLLLHGFPQHWWQWRAVAPVIAARGYRAICPDLRGAGWTVADDPRVERETRQRD
                     VLALLDALHVERAHLVSHDMGAITAMQLTYEHPERWLTAVQLSVPPGFMTFSPRVAPG
                     FRHLPRFIWHHPGDSLRGIFSEAYVAHPLTEATIDAHLAPMSRPEVDAAVRPLTRGMI
                     LPEAVRLARGVYRRRRLTVPTLVVFGRRDRPWTEENLRRICRHPERHADGVEFAFVDD
                     AAHFITDDAPAEVAELAVDWFARAA"
     gene            2028..2372
                     /locus_tag="PL014_10350"
     CDS             2028..2372
                     /locus_tag="PL014_10350"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOT57535.1"
                     /translation="MAYDEDLAGRIRAAVEAATGPEGYREIKMFGGLCWTVNTHMAVG
                     TTGDDLMVHVGTDGVADALEQGARPAMMGERTMGGMVLVASADLQASGAMDAWVRPAV
                     ARARAKPPKKPR"
     gene            complement(2422..3384)
                     /locus_tag="PL014_10355"
     CDS             complement(2422..3384)
                     /locus_tag="PL014_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT57536.1"
                     /translation="MDATDLAQRFSIGGAARLSDGPVARGKQGVVWRLDTDMGRWALK
                     VPFGSPSEEQVASATAFHEAAHAAGVPTPRVLRTDDGAVFAPFNGGQARLYEWVDLLP
                     PDRFLDPAQVGAVVAAIHRVPTPDGALGALDPFFCEPVGADRWDHVLDQLTTEGAPFA
                     SRLAELRDELVALESWIEPPRRLLTCHRDLWADNVLGTADGGVCVIDWEESGPADPSQ
                     ELGYVLFEFAGGDPGRARDLVTAYRDAGGPATVSRRGDFTMLIAQLGHIIELAAKDWL
                     TPNLRSPQRADAVAWISEALDDPHTREVLDSLLAAVDGSGDPGP"
     gene            3536..5134
                     /locus_tag="PL014_10360"
     CDS             3536..5134
                     /locus_tag="PL014_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOT57537.1"
                     /translation="MPTVDLTDTARLERLSDLMVARAPRPDTLVEIHEQRDGILRRLE
                     EARARGLVAVPDLQPLRRNVVTLERHQSSYKSQGGRGTCYAFAAVAAMEAAYKRAHGV
                     ELDLSEEFLFQLNKVAELFPFYLTGTPAHENNCSYSGFQGSSDIVAKVVRSAICDEAA
                     APYRQESDVEAIRTSDPSMGTLGWGASTQEQFDRFEFDERYVPTAARYQARYRVKSMH
                     FLPSASRENVMATIDAGYEVIADIPGHCYLIIGYDLDTQDWLVKNSWGAPGPERWPMA
                     TTTLLGGTYITAVEPLDAPRDMEATWIGRWNMNHDGWPGQLVIRRTQDFRAGDGDFTK
                     LGDYYIDGVARQVNGRTEDDGRQLHFWIADTPGRITPGAQAGQEFRVYQFTREDGLAA
                     GVTWWNGSPFGVTLARSPITSPPVSGTTWQGAWDMNHDGWHGRLDITSTGPLAGTYTP
                     AGGSPVALRDVVGADGYRLSFTIPFSADNVQPFSLFRHTQEGGRFSGTTQWAGMTFGA
                     QGRRALPTELRVEKLVVKRFPPIG"
     gene            5159..6052
                     /locus_tag="PL014_10365"
     CDS             5159..6052
                     /locus_tag="PL014_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57538.1"
                     /translation="MEIADVAHQLYGLPPEEFTAARNACAKAAKDGGDRELAASVTAL
                     RKPTAGAWLLNQLVRRHRGEVDGVLELGVRLRAAQGTLGAADLRALDEQRRQLTRAVA
                     RQAVAIAVAAGRKVSAQVTADVEETLRSAMVDPEAGAALATGLLTDTFSSNGLEPVEV
                     ARVVALGERATGPAAPVPRRAATVSDAAAHARAARALADAEHAVEAAQEHLRHAREES
                     EQARQAAVEARRRREEVESQLAEVRRRLADLETQLAASSQAEDAARRTQVSATRAERA
                     AVESANRARHELTTLLDRQVP"
     gene            complement(6104..>6230)
                     /locus_tag="PL014_10370"
     CDS             complement(6104..>6230)
                     /locus_tag="PL014_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT57539.1"
                     /translation="PIERLASLVALTDFMATYVALGLGLDPSVSRHVTELRDRTS"
BASE COUNT          938 a         2314 c         2159 g          819 t
ORIGIN      
        1 ggtcatccag aacgtgctgc cgagcgtgct ggacgcgacg tacgcccaga tcgcggcggc
       61 cgctcccgac gcgcacgtcg tcgtgaccgg ctacccgcgc ctgttctcgc ccgagtccgg
      121 cgactacttc aatgcctccc ccgccgagca gcaggcgatg aacgacggtg cagacctcct
      181 caactcggtg atcgccgagg ccgcggggga tgccgggttc cagttcgtcg acgtgacgag
      241 ccgcttcgtc gggcacggag tcaacgcccc cgaaccgtgg gtcctcggcg tgaccgaccc
      301 cggccggttc cacccgaacc tcgccggcta cgaggcctac gcggcggcgg tcacctcaca
      361 ggtgaacccc cgagacctgc gctagtccct caccggctca cgggtcggct caggtggctg
      421 cgagggaacg caccacgcgg tcgatcgcgg ggcgcaggtc gagccccggg tcgaccgtga
      481 gccggtgcag cagcagcccg tccagggtcg ccatgagcgc ccgggtcgcg ggcacggggt
      541 cggcgaagcc gagggtgccc accaggtgct cggtccacgc ttcgaactcc cggcgctggc
      601 gccggaggga ctcaccgagc ccgggatccc ccgcgagctc gaggaagagg gcgtagcgcg
      661 cccgggtccg ggcggcgaag ggcccggtct ggagctcggc catggcgcac aacccccgga
      721 ccagctcctc ggcatccgtg accacaggca tggcaccggg atcgaagtcg gcccgctctc
      781 gctcggcgat ccagtcgacg acgccggcga gcagggccct gcgggtgcgg aaccagttgg
      841 aggtcgaccc cggcgggagc ccggcccggg cgtcgacccg cgcgtgggtc agcgcccgga
      901 caccctcggc gccgagcacg gcgaccgccg cctcgagcgc gcgctcccgg gtggacgcag
      961 tcatggacac ctcctgcacc tcggttctac tatgaacata gtgccccgtc cgtgcgaaag
     1021 gagcgcgaca tgccggagcc gaccgcaccg tggtctccgc ccctgcccgc agcacccggt
     1081 ttcgagcacc tcgtcgtcga gacgcccggc ctcaggtcgc acgtcgcggt catcggcgac
     1141 ggcgacggtg acggtgacgg tggtggggac ccggtcctgc tgctgcacgg gtttcctcag
     1201 cactggtggc agtggcgcgc cgtcgccccg gtgatcgccg cccgcggcta ccgcgccatc
     1261 tgccccgacc tgcgcggtgc cggctggacc gtcgctgacg acccgcgtgt cgagcgggag
     1321 acgcggcagc gcgacgtgct cgccctcctc gacgcgctgc acgtcgagcg ggctcatctc
     1381 gtctcccacg acatgggcgc gatcaccgcg atgcagctga cctacgagca ccctgagcga
     1441 tggctcaccg cggtgcagct ctccgtaccg ccggggttca tgacgttcag ccccagggtc
     1501 gccccgggct tccgccacct accccggttc atctggcacc acccgggcga ctccctacgc
     1561 gggatcttct ccgaggcgta cgtcgcccac ccgctgaccg aggccaccat cgacgcccac
     1621 ctggccccga tgagccggcc ggaggtcgac gcggcggtcc gcccactcac gcgcggcatg
     1681 atcctgcccg aagccgtgcg cctggcccgc ggcgtctacc gccgccgccg gctcacggtg
     1741 ccaaccctgg tcgtcttcgg tcgccgcgac cgcccttgga ccgaggagaa cctgcgacgg
     1801 atctgccgcc atcccgagcg acacgccgat ggcgtcgagt tcgccttcgt cgacgacgcc
     1861 gcgcacttca tcaccgacga cgccccggcc gaagtcgccg aactggccgt cgactggttc
     1921 gctcgcgctg cctgacggat gccgcgtgca ggaggcggag tcggggatgg agcacggggt
     1981 tggcgcaccc tcgtcgctag gctcagtgag acggagggag tctcttgatg gcatacgacg
     2041 aggacctggc gggacggatc cgcgctgcgg tcgaggcagc gaccgggccg gaggggtacc
     2101 gcgagatcaa gatgttcggc gggctctgct ggacggtcaa cacccacatg gcggtcggga
     2161 ccaccggtga cgacctcatg gtccacgtcg gcacggacgg cgtcgccgac gccctcgagc
     2221 agggtgcccg ccctgccatg atgggcgaac gaaccatggg cggcatggtg ctggtcgcgt
     2281 cggcagacct gcaagcctct ggtgcgatgg atgcctgggt cagaccggcg gtggcacggg
     2341 cccgggcgaa gccgccgaag aagccgaggt agcgacgccc acagccgacg aagaacccga
     2401 gggcggcgca ctgtcactcc tctacggacc cgggtcgccg ctgccgtcga ccgcagccaa
     2461 gagggagtcg agcacctcgc gggtgtgcgg gtcgtccagg gcctcgctga tccacgccac
     2521 ggcgtcagca cgctgcgggg accgcaggtt gggcgtcagc cagtccttgg cggccagctc
     2581 gatgatgtgc ccgagctgcg cgatgagcat cgtgaagtct ccgcgacgac tcacggtcgc
     2641 cggccccccg gcatcccggt acgcggtgac caggtctcgg gcccgaccgg ggtcaccacc
     2701 ggcgaactcg aaaagcacgt acccgagctc ctggctcggg tctgccgggc cgctctcctc
     2761 ccagtcgatg acgcagactc ccccgtctgc cgtccccagg acgttgtcgg cccacaggtc
     2821 ccggtgacag gtcagcagcc gccgcggcgg ctcgatccag gattccagcg caaccagctc
     2881 gtcgcgcagc tcagccagcc ggctcgcgaa cggcgctccc tccgtcgtca gctgatccag
     2941 cacgtggtcc cagcgatcag cgccgacggg ttcgcagaag aaggggtcca gcgcacccag
     3001 ggctccgtcg ggggtgggga cgcggtggat ggctgccacg acggcgccga cctgagccgg
     3061 atccaggaaa cggtcgggag ggagcaggtc gacccactcg tacagccggg cctgaccacc
     3121 gttgaagggt gcgaacaccg ccccgtcgtc ggtgcggagg acccgagggg tcgggacgcc
     3181 cgcggcgtgg gcagcttcgt ggaacgccgt cgccgaagcc acctgctcct ccgacggcga
     3241 cccgaacggc accttcaggg cccacctgcc catgtcggtg tccagccgcc agacgacacc
     3301 ctgcttgccg cgcgcgacgg ggccatccga caggcgcgca gccccgccga tcgagaagcg
     3361 ctgcgccagg tccgtcgcgt ccatccacgc attcgagcac gtgatgaccc cgacgtcgac
     3421 ccgggcgacc tgtgccggca cacgatccgg cgaccacgct gtatcaggcg cacccgggcg
     3481 cgctcttcac ctccacagcg tgcgcgaccg cgccgctact tccctggagg acacgatgcc
     3541 caccgtcgac ctgaccgaca ccgcccgcct cgagcgcctg agcgacctca tggtcgcgcg
     3601 ggctcctcgg cccgacacgc tcgtcgagat ccacgagcag cgcgacggca tccttcgccg
     3661 cctcgaggag gcccgggctc gtggtctcgt cgcggtcccc gacctgcaac cgctacggcg
     3721 caacgtcgtc acgttggagc gccaccagag ctcgtacaag tcccagggtg gtcgcgggac
     3781 gtgttacgcc ttcgccgccg tcgcggccat ggaagcggcc tacaagcgcg cccacggcgt
     3841 cgagctcgac ctgtccgagg agttcctctt ccagctcaac aaggtcgccg agctcttccc
     3901 gttctacctc acgggcaccc cggcccacga gaacaactgc tcctactcgg gcttccaggg
     3961 ttccagcgac atcgtcgcga aggtcgtgcg gtcagccatc tgtgacgagg cggccgcccc
     4021 ctaccgccag gagtccgacg tcgaggccat ccgcacgtcg gacccgtcga tggggactct
     4081 ggggtggggg gcgagcaccc aggaacagtt cgatcggttc gagttcgacg agcgctacgt
     4141 cccgaccgct gcccgctacc aggcccgcta ccgggtgaag tcgatgcact tcctccccag
     4201 cgcgtcgcgc gagaacgtca tggcgaccat cgacgcgggg tacgaggtca tcgccgacat
     4261 cccgggccac tgctacctca tcatcggcta cgacctggac acgcaggact ggctggtgaa
     4321 gaactcgtgg ggcgcccccg gaccggaacg ctggcccatg gcgaccacca cgctcctcgg
     4381 tggcacctac atcaccgcag tggagccgct ggacgcacct cgggacatgg aggccacctg
     4441 gatcggccgc tggaacatga accacgacgg gtggccgggg cagctcgtca tccggcgtac
     4501 ccaggacttc cgcgccggcg acggtgactt caccaagctc ggggactact acatcgacgg
     4561 ggtcgcccga caggtcaacg ggcgcaccga ggacgacggt cgccagctgc acttctggat
     4621 cgccgacacc ccgggtcgga tcacccccgg ggcgcaggcg ggacaggagt tccgggtcta
     4681 ccagttcacc cgtgaggacg gcttggctgc cggtgtcacg tggtggaacg gctccccgtt
     4741 cggcgtcacc ctggcgcgat cgcccatcac gtcgccgccc gtctcgggca cgacctggca
     4801 gggcgcctgg gacatgaacc acgacggctg gcacggccgg ctcgacatca cgtcgaccgg
     4861 gccgctcgcg gggacctaca cccctgccgg cggatccccc gtggcgctgc gcgacgtggt
     4921 cggcgccgac ggctaccggc tctcgttcac gatcccgttc tcggcggaca acgtgcagcc
     4981 cttcagcctc ttccggcaca cccaggaggg cggtcggttc tccggcacca cccagtgggc
     5041 ggggatgacc ttcggggccc agggccgccg ggcactgccc accgagctgc gcgtggagaa
     5101 gctcgtcgtc aagcggttcc cgccgatcgg ttgagcacag ccgtggcacc atcacccgat
     5161 ggagatcgcg gacgtcgccc accagctgta cggcctgccc ccggaggagt tcaccgccgc
     5221 ccgcaacgcc tgcgcgaagg cggccaagga cggcggtgac cgcgagctgg cggcctccgt
     5281 gacggccctg cgcaaaccga cggcgggcgc gtggctgctc aaccagctgg tgcgccggca
     5341 ccgcggtgag gtggacgggg tgctcgagct cggcgtccga ctgcgcgcgg cccagggcac
     5401 gctcggggcc gccgaccttc gagccctgga cgagcagcgg cggcagctga ctcgagccgt
     5461 cgcccggcag gccgtcgcga tcgccgtggc tgccggcagg aaggtctcgg cccaggtgac
     5521 ggcagacgtc gaggagacgt tgcgctcggc gatggtggac ccggaggccg gcgccgccct
     5581 cgcgacgggg ctgctcaccg acaccttcag ctccaacggg ctggagccgg tggaggtggc
     5641 gcgggtggtg gccctcggcg agcgcgccac gggccccgcc gctccggtcc cgagacgagc
     5701 cgccaccgtc tcggatgccg cggcgcacgc ccgcgccgcg cgcgccctcg cggacgccga
     5761 gcacgcggtc gaggcagcgc aggagcacct gcgccacgca cgcgaggagt cggagcaggc
     5821 caggcaggcg gcggtcgagg cccgtcgccg ccgtgaggag gtcgagtccc agctggccga
     5881 ggtccgccgt cgcctcgccg acctcgagac gcagctcgca gcgagcagcc aggccgagga
     5941 tgccgcccgg cgcacccagg tgtccgcgac cagggccgag cgtgcggcgg tcgagtcagc
     6001 gaaccgagcg cgtcacgagc tgacgaccct cctcgaccgc caggtcccgt agcgccggcc
     6061 catggcatga ggcggacacc tcaggcgtcg atcaccccac aggtcaggag gtgcggtcgc
     6121 gcagctccgt gacgtgccgc gacaccgacg ggtcgagccc gagcccgagc gccacgtagg
     6181 tggccatgaa gtcggtcagg gccacgagcg aggccagccg ctcgatcggc
//
LOCUS       DAOSOC010000147         6194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12757_length_6194_cov_5.812836, whole genome shotgun sequence.
ACCESSION   DAOSOC010000147 DAOSOC010000000
VERSION     DAOSOC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12757_length_6194_cov_5.812836"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2070)
                     /locus_tag="PL014_10375"
     CDS             complement(<1..2070)
                     /locus_tag="PL014_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT57540.1"
                     /translation="MTDRDREAQSFDLLHPVVQHHVVNSLGWPGLRPLQTASIVPVLR
                     GDDALLVAATAGGKTEAAMLPLLTRMADDEWPGLSVLYVCPLKALLNNLEPRLATYAG
                     WLGREASVRHGDTAAGARQRQATSRPSVLLTTPESLEAMLVSTLLDAPHMFGDLRAVV
                     VDEVHAFAGDDRGWHLLAVLDRLGALAGRPLQRIGLSATVGNPDVLLQWLQGRERSRP
                     RTVIAPPTASSTAPELALDHVGNVGNAAKVVNGIHLGEKRLVFADSRRTVEGLAVALR
                     GLGTQTYVSHSSLSVDERRRAETAFAEARDCVIVSTSTLELGIDVGDLDRVVQLGAPN
                     TVASVLQRLGRTGRRPGSTRNLTFLATEDDELLRAAGLLLLLDEGFVEPVVAPPEPLH
                     VAAQQLLGVALQKGRISLAEESIWICGLGLADEVRLAEICAWLVETGHLDTDQGLAFI
                     GPRAEARYGRRNFMDLLAVFTAAPEVVVVHGRREVGSVDPMLLMAKVTGPRVIALAGR
                     PWLVTHIDWRRRRAYVEPSDRAGRSRWTGEPRAYSLELSDAVRRVLLGATPVGAELTT
                     RAMNRLGSLREQYRHRVAPDASVVTEDDGRVRWWTFAGAGANAVLTAALDAVAPDLLD
                     QGTFGNLNIALRSDATAVAVGSAVRRARAEFGSDLVGVTPPVSEQALAKLKFAELLPP
                     HLALGTLS"
     gene            complement(2063..3403)
                     /gene="brxD"
                     /locus_tag="PL014_10380"
     CDS             complement(2063..3403)
                     /gene="brxD"
                     /locus_tag="PL014_10380"
                     /inference="COORDINATES: protein motif:HMM:NF033438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HOT57541.1"
                     /translation="MAGAVSNRRRGEVIGALRRGTVPESGLDLLAVGLDRFSVALDAE
                     LATVAGGGAMFKAVRGEYGAGKTFFTRHLAERAMRQGFATAEVQVSETETPLHRLETV
                     YRRITESLRTSSMPPSAFRPVLDSWLFTLESDAIAADPSLEHADEASVTQAVNALLER
                     RLASVSSRTPVFAQALRGYRTAVVSGDAATADGLAAWMGGQPQVAASAKRAAGIRGEL
                     DHFGAMGFLQGLLAVLRDAGHPGLVLVLDEVETLQRVRGDVRDKAFNALRQLIDEIDA
                     GRFPGLYLLVTGTPAFYEGPSGVARLAPLAQRLATDFATDARFDNPRAVQVRLPGFDH
                     DALVSLGVKVRDLYSGGSAASARVASTVDDAYVADLATAVAGRLGGRVGVAPRVFLKK
                     LVADVLDRVDQFEDFDPREHYALTVGVDELTTEERVARGGSGLDGIDLDLSDDD"
     gene            complement(3403..>6194)
                     /gene="pglZ"
                     /locus_tag="PL014_10385"
     CDS             complement(3403..>6194)
                     /gene="pglZ"
                     /locus_tag="PL014_10385"
                     /inference="COORDINATES: protein motif:HMM:NF033446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HOT57542.1"
                     /translation="PTLVTAATTPMLLAMVDGARHRNYTSGILGVLGQPAQSQAEEVH
                     HDGQLVRIRPAESALAVREALLEHVEGDWLVVVTDRDEQDLGAGILAHLVGSRLWRPD
                     AWEALRQSFSASGVAPALASRPGSRDLATAILAARPPQGWPPAPAGVLTRAHAMTCVA
                     RTHLGLVGDVVDAITVLSWSVLPQAADALADLRQTYGPTLADALVDWVAESTGPAATP
                     VRKLLEAGAVADLVPVGLALHLITSEGHDLDESQVAKLASVRLEQALGKPLPTRDSLA
                     AHGSAAVAVVADLARTERNEAHVARVVSRADTILTSLDATALAVHSDVMPSGFRQRLA
                     LLAAALHRGVSLHGAGDPIAEASRAIETAWTLCARHRLARRDTAQWLAFDSAVKLWRW
                     LTTPEIPGSADLPARVRAHLDHGSWADIAINDVDTGVDDPDLSESLHAVYAAAFARRD
                     HEERAFAARLAALTAAQPHGALVPQDTGAEPIWNLESLLPSLVIPMAKQTPILLVVMD
                     GMSAAAANEVLADVTGELGWVEAGATPGATRRAGGLAVLPSVTEVSRASLLCGSLQQG
                     GQGVERTGYQALTARGSRLRAELFHKKGIDTTRPGALVADGVGAAIDDAKGIPLVTVV
                     LNTIDDALDRSDPIGTVWTADAVKHLAPLLTRARAAGRTVVMTSDHGHVVERRRGTQR
                     FAPDLTSGRSRGMSSPAAEDEVAVEGPRVLTSDHRAVLAVSEGLRYGPLKAGYHGGAS
                     AQEVVVPVVVLLPDQATNELRLPALPPQAPLWWSTAEPGSSDLPTMPTLSTPPTGIRP
                     TDGRRGKLPDGPTLFDDPQPSAAEAAPVASLGSQVVASEVYAAQRQVLSRLPIRDDQV
                     AALLDALTAAAGQRLPRTVVAAALAVPAFRLDGALSQVRQLLNIEGYNVVGVDPDGQT
                     VVLDVPLLRDQFEVR"
BASE COUNT          851 a         2141 c         2391 g          811 t
ORIGIN      
        1 ggacagcgtg ccaagcgcca ggtgcggagg caggagttcg gcgaacttga gcttcgcgag
       61 cgcctgctcg ctgaccggag gcgtcacgcc cacgaggtcc gagccgaact ccgcccgggc
      121 ccggcgcacc gccgacccca ccgcgaccgc cgtggcatcg gatcgcagcg cgatgttcag
      181 gttgccgaac gtgccctggt cgagcaggtc aggtgccacc gcgtcgaggg cagcagtgag
      241 cactgcgttc gcgcctgccc ccgcgaaggt ccaccagcga actcgcccgt cgtcctcggt
      301 gaccacgctg gcgtccggcg cgacccggtg tcggtactgc tcacgaagag acccgagccg
      361 gttcatcgcc cgggtggtca actcggcacc cacgggcgtg gctccgagca gcacccggcg
      421 cacggcatcc gacagctcga gcgagtaggc gcgaggttcg ccggtccatc gtgagcgacc
      481 ggcccggtcg ctcggctcga cgtatgcccg gcgccgccgc cagtcgatgt gggtgaccag
      541 ccacgggcga ccggcgaggg cgatgacccg cgggccggtg accttggcca tgagcagcat
      601 cgggtcgacg ctgccgactt cccgccggcc gtgaaccacc acgacctcgg gcgccgcggt
      661 gaagacggcg agcaggtcca tgaagttgcg ccgaccatag cgagcctccg cccgggggcc
      721 gatgaacgcc agcccctggt cggtgtcgag gtggccggtc tccacgagcc acgcgcagat
      781 ctccgccagc cgcacctcat cggccaggcc gagcccgcag atccagatgg actcctcggc
      841 gagcgagatc cgacccttct ggagagccac ccccagcagt tgctgcgcgg ccacgtgcag
      901 cggctcgggg ggagccacga ccggctcgac gaagccctcg tccagcagca gcaacaaacc
      961 cgcggcgcgg agcagttcgt cgtcctcggt ggcgaggaag gtgaggttgc gggtcgaccc
     1021 ggggcgccgc ccggtgcgcc cgagccgttg cagcacggac gcgacggtgt tcggcgcgcc
     1081 gagttgcacg acgcggtcga ggtcgccgac gtcgataccg agctcgaggg ttgaggtcga
     1141 gacgatgacg cagtcgcgcg cctcggcgaa ggccgtctcg gcccggcgcc gctcgtccac
     1201 cgacaacgag gagtgggaga cgtacgtctg cgtccccaac ccacgcaggg caactgcgag
     1261 cccctcgacg gtcctcctgg agtcggcgaa cacgaggcgt ttctccccga ggtggatgcc
     1321 gttgacgacc ttcgcggcgt tgcccacgtt cccgacgtgg tccagagcca gttcgggtgc
     1381 tgtcgacgac gcagtcggtg gcgcgatgac ggtgcgcggc cgcgagcgct ctcgcccctg
     1441 gagccactgg agcagcacat ccgggttgcc gacggtggcg gacagaccga tccgctggag
     1501 cgggcgcccg gccagcgcgc ccagccgatc gagcacggcg agaaggtgcc agccgcggtc
     1561 gtcgcccgcg aaggcgtgca cctcgtcgac gacgacggcc cgcaggtcac cgaacatgtg
     1621 cggcgcgtcg agcagcgtcg agacgagcat cgcctcgagc gactcgggcg tcgtgagcag
     1681 gaccgagggc cgcgacgtgg cctgtcgctg gcgggcccct gcagcggtgt cgccgtggcg
     1741 cacgctggcc tcccggccga gccacccggc atacgtcgcg agccggggtt cgaggttgtt
     1801 gagcagcgct ttgagggggc acacgtacag caccgacagg cccggccact catcgtccgc
     1861 catgcgggtg aggagcggca gcatcgcggc ctcggtcttg ccgccggccg tggcggcgac
     1921 gagcagcgcg tcgtccccgc gcaacaccgg gacgatcgac gcggtctgca gcggccgcag
     1981 ccctggccag ccgagcgagt tgacgacgtg gtgctgcacg accgggtgca gcaggtcgaa
     2041 ggactgcgcc tcccggtcgc ggtcagtcat cgtccgacag gtcgaggtcg ataccgtcga
     2101 gccctgagcc gccgcgggcg acccgctcct ccgtagtcag ctcgtcgaca ccgacggtga
     2161 gggcgtagtg ctccctcggg tcgaagtcct cgaactggtc gacccggtcg agaacgtcgg
     2221 cgacgagctt cttgaggaag acccggggtg ccaccccgac ccggccaccg agccgtccgg
     2281 cgaccgcggt ggcgaggtca gcgacgtatg cgtcatcgac cgtcgaggcc accctggcgc
     2341 tcgcggcgct cccgccggag tagaggtcgc ggaccttcac accgagggag acgagagcgt
     2401 cgtggtcgaa gccgggcagg cgcacctgca cggcccgcgg gttgtcgaag cgggcgtcgg
     2461 tggcgaagtc ggtcgcgagt cgctgcgcga gcggcgccag gcgtgcgacc ccggagggcc
     2521 cctcgtagaa ggccggtgtc ccggtcacga gcaggtagag ccccgggaag cggcctgcgt
     2581 cgatctcgtc gatgagctgg cgcagcgcgt tgaacgcctt gtcccgcacg tcgccacgca
     2641 cccgctggag cgtctcgacc tcgtcgagca cgagcacgag acccgggtgc ccggcatcgc
     2701 gcagcacggc gagcagccct tggaggaagc ccatcgcccc gaagtggtcg agctcgccgc
     2761 ggatgccggc cgctcgcttc gccgatgccg cgacctgggg ttgtccaccc atccaggccg
     2821 cgagtccgtc ggccgtcgcg gcgtctccgc tgacgacagc ggtgcggtag ccgcgcaggg
     2881 cctgggcgaa caccggtgtc cgggagctga ccgatgcgag ccgtcgttcg agcagggcgt
     2941 tgacggcctg ggtcaccgac gcctcgtcgg cgtgctccag cgacgggtcg gccgcgatgg
     3001 catccgactc gagggtgaac agccaggagt cgaggacggg ccggaaggcg ctgggtggca
     3061 tcgacgacgt ccgcagcgac tcggtgatcc gccggtagac ggtctcgagg cggtgcaggg
     3121 gcgtctcggt ctcggagacc tgcacctccg cggtggcgaa gccctggcgc atcgcccgct
     3181 cggccaggtg gcgcgtgaag aaggtcttgc cggcgccgta ctcaccgcgc acggccttga
     3241 acatcgcgcc gccgccggcg acggtggcga gctccgcgtc gagggccacg gagaagcggt
     3301 cgagcccgac ggcgagcagg tcgaggcccg actcgggcac cgtgccccgc cggagcgcgc
     3361 cgatgacctc gccccgacgc cggttggaga cggcgccggc catcaccgca cctcgaactg
     3421 gtcgcgcagc agcggcacgt cgagcacgac ggtctgcccg tcggggtcga cgccgacgac
     3481 gttgtagccc tcgatgttga gcagttggcg cacctggctc agggcgccgt cgaggcggaa
     3541 cgccggcacc gccagggccg cggccacgac ggtgcgcggc aggcgctggc ccgcggcagc
     3601 ggtgagggcg tccagcaggg cggcgacctg gtcgtcgcgg atgggcaggc gggacagcac
     3661 ctgtcgctgg gcggcgtaca cctcgctcgc cacgacctgc gacccgagcg aggcgacggg
     3721 tgcggcctcg gcggcgctcg gctggggatc gtcgaacaac gtgggtccgt cgggcagttt
     3781 cccacggcgc ccgtcagtgg ggcggatgcc ggtgggcggg gtcgacaggg tgggcatggt
     3841 gggcaggtcg gaggatcccg gctcggccgt cgaccaccac aaaggtgcct ggggtggcag
     3901 ggcgggcagt cggagctcgt tggtcgcctg gtccgggagc agcacgacca cgggcacgac
     3961 gacttcctgg gcgctcgcgc caccgtggta gccggccttg agcgggccgt agcgcagccc
     4021 ttcgctgacg gcgagcaccg cccggtggtc gctggtgagc acccgcggtc cctcgacggc
     4081 gacctcgtcc tcggcggccg gtgacgacat cccccgcgag cgcccgctcg tgaggtcggg
     4141 tgcgaagcgt tgggtgccgc gtcgccgctc cacgacgtgg ccgtggtcgg acgtcatgac
     4201 gacggtccgc ccggcggccc gcgcccgggt gagcagcggt gcgaggtgct tgacggcatc
     4261 cgcggtccac accgtgccga tcgggtcgct gcggtcgagc gcgtcgtcga tcgtgttgag
     4321 cacgaccgtg accaggggaa tgcccttcgc gtcgtcgatg gccgccccca cgccgtcggc
     4381 gaccaaggca ccggggcggg tggtgtcgat gcccttcttg tggaacagct ctgcacggag
     4441 cctgctgccc cgggcggtca gggcctggta cccggtgcgt tcgacgccct gcccgccctg
     4501 ctggagtgag ccgcagagca gcgacgcccg gctcacctcg gtgaccgacg gcagcacggc
     4561 aaggccgccg gcgcggcggg tggcgccggg tgtcgccccg gcctcgaccc agccgagttc
     4621 tccggtgacg tcggcgagga cctcgttcgc tgctgcggcg ctcatgccgt ccatgacgac
     4681 gaggaggatc ggtgtctgct tcgccatcgg gatgacgagg gagggcagga gtgactcgag
     4741 gttccagatc ggctccgccc cggtgtcctg tggcaccaag gccccgtgcg gctgggcggc
     4801 ggtcagggcc gccagtcgtg cggcgaaggc gcgctcctcg tggtcgcgcc gggcaaacgc
     4861 cgcggcgtag acggcgtgca gcgactcgct gaggtcgggg tcgtcgacgc cggtgtcgac
     4921 gtcgttgatg gcgatgtcgg cccacgaccc gtggtcgagg tgggcccgca cccgcgccgg
     4981 gaggtccgcg ctgccgggga tctcgggggt ggtcagccag cgccacagct tgacggcgga
     5041 gtcgaaggcc agccactggg ccgtgtcgcg tcgggcgagc cggtgccggg cgcacagcgt
     5101 ccacgccgtc tcgatggcgc gggaggcctc ggcgatcggg tcgcccgcgc cgtgcaggga
     5161 caccccgcgg tgcagggcgg cggcgagcag cgcgaggcgt tgccggaagc cgctcggcat
     5221 cacgtcggag tgcacggcga gcgcggtggc gtcgagcgac gtgaggatcg tgtcggcccg
     5281 ggagaccacc cgtgcgacgt gggcttcgtt gcgctcggtg cgggccaggt cggcgaccac
     5341 ggcgacggcg gcggagccgt gggcggcgag gctgtcgcgt gtcggcagcg gctttccgag
     5401 ggcctgttcc agccgcaccg aggcgagctt ggcgacctgg ctctcgtcga ggtcgtgccc
     5461 ctccgaagtg atcaggtgca gcgcgagccc caccggcacg agatcggcga cggccccggc
     5521 ctcgagcagc ttgcggaccg gggtggcggc cgggccggtc gactcggcga cccagtcgac
     5581 cagggcgtcg gcgagggtgg ggccgtaggt ctgccgcagg tcggcgaggg catcggcggc
     5641 ctggggcagc accgaccagc tgagcaccgt gatggcgtcg acgacgtcgc cgacgaggcc
     5701 gaggtgggtg cgggcgacgc acgtcatggc gtgggcccgg gtgagcaccc ccgccggcgc
     5761 gggcggccac ccctgcgggg ggcgggccgc gaggatcgcg gtggcgaggt cgcggctgcc
     5821 ggggcgggag gccagtgcgg gggcgacccc cgacgcggag aacgactggc gcagggcctc
     5881 ccaggcatcc ggtcgccaga gccggctgcc gacgaggtgg gcgaggatac cggcgccgag
     5941 gtcctgctcg tcgcggtcgg tcacgacgac gagccagtcg ccctcgacgt gctcgagcag
     6001 ggcctcgcgc acggcgagcg ccgactcggc agggcggatc cgtacgagct gtccgtcgtg
     6061 gtggacctcc tccgcctggc tctgtgcggg ttggccgagc acgccgagga tgccgctggt
     6121 gtagttgcgg tgccttgccc cgtcgaccat ggcgagcagc atcggggtgg tcgcggcggt
     6181 cacgagcgtg ggga
//
LOCUS       DAOSOC010000148         6162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12835_length_6162_cov_10.148354, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000148 DAOSOC010000000
VERSION     DAOSOC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12835_length_6162_cov_10.148354"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="PL014_10390"
     CDS             complement(<1..160)
                     /locus_tag="PL014_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010058817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOT57543.1"
                     /translation="MPPKKKKISGYINLQIQAGAATPAPPVGPALGQHGVNIMEFVKA
                     YNAATEHQR"
     gene            complement(241..1008)
                     /gene="nusG"
                     /locus_tag="PL014_10395"
     CDS             complement(241..1008)
                     /gene="nusG"
                     /locus_tag="PL014_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOT57544.1"
                     /translation="MAEGLDAAVGVDSADDVDTSADDVHASADDDDAETPVASATDED
                     GDRLDVVDPVQAFKDDLRSKVGDWYVVHSYAGYENRVKANLETRITSLNMEDYIFEIQ
                     VPMEEVTEIKGGQRKLVRRVRMPGYVVVRMDLTDESWGAVRHTPGVTGFVGNAHQPVP
                     LTFDEVYSMIKPADLEAAAAAAAAGGTASASSAGRETTVVDFEVGESVTVMDGPFETL
                     PATISEINADTQKLKVLVSIFGRETPVELSFSQVTKL"
     gene            complement(1175..1432)
                     /gene="secE"
                     /locus_tag="PL014_10400"
     CDS             complement(1175..1432)
                     /gene="secE"
                     /locus_tag="PL014_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOT57545.1"
                     /translation="MGQTRTPSKPAKGKESGGNPISRWVKALTLFVSQILDELRKVVR
                     PTRNELWNYTLVVIVFVTVMMALVSGLDLGFSKLVGLVFGS"
     gene            complement(1546..1618)
                     /locus_tag="PL014_10405"
     tRNA            complement(1546..1618)
                     /locus_tag="PL014_10405"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1583..1585),aa:Trp,seq:cca)
     gene            1796..3094
                     /locus_tag="PL014_10410"
     CDS             1796..3094
                     /locus_tag="PL014_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOT57546.1"
                     /translation="MSTDTAATTPLAERSPQELQAYLEEQRAAYDALCERGLTLDLTR
                     GKPSSAQLDLSDRLLSLPEGVKDADGVDVRNYGGLTGLRELREMFAELLWVDPDQLVA
                     GGSSSLTMMHDVLVQHLLHGGLDSPRPWKDEEKVTFVCPVPGYDRHFTMLASYGIEMV
                     SVPMLADGPDVAAVEALVRDDPSVKGMWIVPTYANPAGSVVTQEVASRLASMPTAAPD
                     FRIFWDNAYALHHLTADEAKSADILSLSSAAGHPHRPIMFASTSKITYAGAGVAFLAA
                     SRENIAAYLGHQQFASIGPDKVNQLRHIQFFGSAQGVRDHMARHREILAPKFAAVDAA
                     LTAELGGLGIAQWTRPAGGYFVSLDVPDGCAARVVALAKAAGIALTPAGASFPHGQDP
                     RDRNIRLAPSFPQLAEVETAMAGVATCVALAAAEQLTAVG"
     gene            3099..3614
                     /locus_tag="PL014_10415"
     CDS             3099..3614
                     /locus_tag="PL014_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HOT57547.1"
                     /translation="MSEPQESLTTDLGAYSLDDEDQLQPGDTLDDDDVDVLDRGYSPP
                     DTDRGSRAHGVTASEQHRDETIEQRIAQEVPDPTSAYGAPDNESGLDERDRIGGDDPD
                     SIDAEDDWIGDGEVGGLRAGRLVAPDRGVGEDSESLLIGEDVGIDGAAASAEEAAMHV
                     IERLPEDNDSE"
     gene            3611..4657
                     /locus_tag="PL014_10420"
     CDS             3611..4657
                     /locus_tag="PL014_10420"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOT57548.1"
                     /translation="MSEATAASQRSVTDLPKAHLHLHFTGSMRVETVRDLAEQHGIRL
                     PAALTHDWPPHFDAADDRGWFRFQRLYDAARACVRGEADMRRIVREAALDDGAEGSRW
                     LELQIDPTSYAPFVGGITPAIEIVLDEARAVSAESGAAQVAVVVAASRMRHPLEARTL
                     ARLAARYSGSGPGTVVGFGLSNDERRGVTAEFGPAFAIADRAGLALLPHAGELLGAQA
                     VAETLDSLRPDRLGHGVRSVEDPAVLDRVVAAGVALEVCPGSNVALGVYVDAHEVPLR
                     QLLDAGATVALGADDPVIFGSRLAQQYTTAREVHGIDDAGLAGLALGSLRASRAPSDV
                     VAAAVADVDAWLRG"
     assembly_gap    4669..4768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5219..5707
                     /locus_tag="PL014_10425"
     CDS             5219..5707
                     /locus_tag="PL014_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57549.1"
                     /translation="MLARLEAGAGQMRLDTISDALVGTGFMLCVTFSQSEPDPSPDDA
                     ASGCAAASDDEPQIQVVPRRVPPEAWSPTDLVAAVRGGGRRFPAHRKVYAVTNPPLWW
                     WMHEFFNGPTEKPKWYAPVPRPDLTLLLGDLGDDDEGDDHGDSSPDDAAAAPHGDEHD
                     AA"
     gene            complement(6048..>6162)
                     /locus_tag="PL014_10430"
     CDS             complement(6048..>6162)
                     /locus_tag="PL014_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57550.1"
                     /translation="QKTAYEIADLGRTVRDGVHDVFGVTLVNEPVFVGHSL"
BASE COUNT          870 a         2140 c         2131 g          921 t
ORIGIN      
        1 cgcgctggtg ctcggtcgcc gcgttgtacg ccttgacgaa ctccatgatg ttgacaccgt
       61 gctgaccgag cgcggggccg accggcggcg ccggggtggc cgcgccggcc tggatctgca
      121 ggttgatgta cccggagatc ttcttcttct tggggggcat gttctgtcct tgtggtggtg
      181 gtgacgggcc gacgccgtgg gcgatgaccc ctttgcagcc ccgaaccccg gggcagggtc
      241 ttacagcttg gtgacctggc tgaacgacag ctcgaccggg gtctcgcgac cgaagatcga
      301 cacgagcacc ttgagcttct gggtgtcggc gttgatctcg gagatcgtgg cgggcagggt
      361 ctcgaagggc ccgtccatga cggtgacgga ctcgccgacc tcgaagtcga cgaccgtggt
      421 ctcgcgaccc gcgctcgacg cggatgccgt gccgcccgct gccgcggcgg ccgcagcggc
      481 ctcgaggtcg gccggcttga tcatcgagta gacctcgtcg aaggtcagcg gcaccggctg
      541 gtgcgcgttc ccgacgaacc cggtgacccc gggggtgtgg cgcacggcgc cccacgactc
      601 gtcggtgagg tccatgcgga cgacgacgta tcccggcatc cggacccggc ggacgagctt
      661 gcgctggcca cccttgatct cggtgacctc ctccatcggg acctggatct cgaagatgta
      721 gtcctccatg ttcaaggagg tgatgcgggt ctcgaggttg gccttcacgc ggttctcgta
      781 gccggcgtag gagtgcacga cgtaccagtc gccgaccttg ctgcgcaggt cgtccttgaa
      841 cgcctggacc gggtcgacca cgtcgaggcg gtcgccgtcc tcgtcggtcg cggaggcaac
      901 cggggtctcg gcgtcgtcat cgtcggcgga agcgtgcacg tcatctgcgg aggtgtccac
      961 gtcgtcggcg ctgtcgacac ccacggcggc gtccagcccc tcggccaccg agtccgcctc
     1021 acgcgacccc tcggtcacgg tgtcgggcag cacctcgggc gcgtcgtcgg acgctgcgtc
     1081 ggggttgtcg gtccactggt cggacacggg aaacctcttc cttgttcggt gaatgccgga
     1141 tggtgcggcc gtgatggcgc cttgcggcgc ggcgtcagga gccgaacacc aacccgacga
     1201 gcttgctgaa gcccaggtcg agcccggaca cgagggccat catcacggtg acgaagacga
     1261 tgacgacgag cgtgtagttc cacagctcgt tacgagtcgg gcggacgacc ttgcgcagct
     1321 cgtcgaggat ctggctgacg aacagggtca gtgccttgac ccaccgggaa atggggttgc
     1381 cgcccgactc cttgcccttg gcgggcttgg agggagtcct ggtctgaccc acgtcagtca
     1441 cggtgcctct tcacgttcgt tgccatgtcg gtgcctttgc gcgcggcgcg accgcgaagg
     1501 cacccatcgt tcggatgcag tcgtggacga accacacgtg cgacgcaggg caggagggac
     1561 tcgaaccccc aaccgccggt tttggagacc ggtgctctac caattgagcc actgccctac
     1621 gagtgtcccg gtcgggtggg gcactcgccc ggtgcgaccg tcaccctcgg gcacgccgaa
     1681 ggatggtgat cacaccgaag agcaagcata cgcgacgccc ccacccgggc gcgaactcgc
     1741 tcgcgactgc ggcgctcgtg ggcgggtccg gtccctgctc tgccacgata tgggggtgag
     1801 caccgacacc gcagccacca cccccctcgc cgagcgttcc ccccaggaac tgcaggccta
     1861 cctcgaggag cagcgcgcag cctacgacgc actgtgcgag cgcggcctga cgctcgacct
     1921 cacccgtggc aagccgagca gcgcccagct cgacctgtcc gacaggttgt tgtccctgcc
     1981 cgagggggtc aaggacgccg acggtgtcga cgtgcgcaac tacgggggcc tgaccggcct
     2041 gcgggaactg cgcgagatgt tcgccgagct gctgtgggtc gaccccgacc agctcgtcgc
     2101 cggtggcagt tccagcctga cgatgatgca cgacgtgctc gtccagcacc tgctgcacgg
     2161 cgggctggac tcgcccaggc cgtggaagga cgaggagaag gtcaccttcg tctgtccggt
     2221 gcccgggtac gaccggcact tcacgatgct cgcctcctac ggcatcgaga tggtgagcgt
     2281 gccgatgctg gccgacggcc cggacgtcgc cgccgtcgag gccctggtgc gcgacgaccc
     2341 gtcggtcaag ggcatgtgga tcgtcccgac gtacgccaac ccggcagggt ccgtggtcac
     2401 gcaggaggtc gcgagccggc tggcgtcgat gcccacggcg gcccccgact tcaggatctt
     2461 ctgggacaac gcgtacgcgc tgcaccacct caccgccgac gaggcgaagt cggccgacat
     2521 cctctccctg tcctcggccg ccgggcaccc gcaccgaccg atcatgttcg cgtcgacgag
     2581 caagatcacc tacgccggcg ccggggtcgc gttcctcgcc gcgtcccgcg agaacatcgc
     2641 cgcctacctg gggcaccagc agttcgcctc gatcggcccc gacaaggtca accagctccg
     2701 ccacatccag ttcttcggct cggcgcaggg tgtgcgtgac cacatggccc gccaccgcga
     2761 gatcctcgct ccgaagttcg ccgcggtgga tgccgcgttg accgccgagc tgggcggcct
     2821 gggcatcgcg cagtggaccc gcccggccgg cgggtacttc gtctccctcg acgtcccgga
     2881 cggctgcgcg gcccgcgtcg tcgccctggc gaaggccgca ggcatcgccc tgacccccgc
     2941 gggggcatcg ttcccgcacg gtcaggaccc ccgcgaccgc aacatccgac tggcaccgtc
     3001 cttcccccaa ctggccgagg tcgagacggc catggcgggg gttgcgacgt gcgtcgccct
     3061 cgccgccgct gagcagctca ccgccgtagg ctgagggcat gtccgaaccc caggaaagcc
     3121 tgaccaccga cctcggcgcc tacagcctcg atgacgagga ccagctgcag cccggggaca
     3181 ccttggacga cgacgacgtc gacgtcctcg accgcggcta ctccccgccg gacaccgacc
     3241 gcgggtcacg cgcccacggc gtcacggcat ccgaacagca ccgcgacgag accatcgagc
     3301 agcggatcgc ccaggaggtg ccggacccga cgtcggccta cggtgccccc gacaacgagt
     3361 cgggtctgga cgagcgggac cgcatcggcg gcgacgaccc cgacagcatc gacgccgagg
     3421 acgactggat cggcgacggc gaggtcggcg gactgcgggc cggtcgcctc gtcgcccccg
     3481 accgcggggt gggcgaggac agcgagagcc tcctgatcgg cgaggacgtc ggcatcgacg
     3541 gtgcggccgc ctcggccgaa gaggccgcga tgcacgtcat cgagcgactg cccgaggaca
     3601 acgactccga gtgagtgagg cgaccgctgc gtcccagcgg tccgtgacgg acctgcccaa
     3661 ggctcacctg cacctgcact tcaccgggtc catgcgggtg gagaccgtcc gcgacctcgc
     3721 cgagcagcac ggcatccgac tgcctgcggc cctgacccac gactggccgc cccacttcga
     3781 cgccgcggac gatcgcggct ggttccgctt ccagcggctg tacgacgcgg cccgcgcgtg
     3841 tgtgcgcggg gaggccgaca tgcgccggat cgtgcgcgag gcagcgctcg acgacggcgc
     3901 tgaggggtcg cgctggctcg agctgcagat cgacccgacg tcgtacgcgc cgttcgtcgg
     3961 cggcatcacc ccggcgatcg agatcgtgct cgacgaggcc cgggcggtct ccgccgagtc
     4021 gggggccgcg caggtcgctg tcgtcgtggc ggcgagccgg atgcggcacc cgttggaggc
     4081 gcggacgctg gccaggctgg cggcgcggta ttcgggatcg gggccgggca ccgtggtcgg
     4141 cttcggcctc tccaacgacg agcgccgcgg ggtgacggcc gagttcgggc cggccttcgc
     4201 gatcgcggac cgggcggggc tggccctgct cccccatgcc ggtgagttgc tcggggcgca
     4261 ggccgtcgcc gagaccctgg actcgctgcg cccggaccgg ctcggccacg gggtccgcag
     4321 cgtggaggac ccggcggtgc tcgaccgggt cgtcgccgct ggcgtggcgc tcgaggtgtg
     4381 ccccgggtcg aacgtggccc tcggggtgta cgtggacgcg cacgaggttc cattgcgaca
     4441 gttgctggat gccggggcga ccgttgcgct cggagccgac gacccggtga tcttcgggtc
     4501 gcggctggcg cagcagtaca cgaccgcgcg cgaggtgcac ggcatcgacg acgccggcct
     4561 ggccgggttg gcgctggggt cgctgcgggc cagccgggca ccctcggacg tggtcgccgc
     4621 ggccgtggcg gacgtcgatg cctggctgcg cggctgagtc ggcgcggcnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gccgcggccg tggcggacgt cgatgcctgg
     4801 ctgcgcggct gagtcggcgc ggctgacgcg ctgcggaggg cgttcctgag agcccgaggg
     4861 gggtgggggc caggggacgt ccggatgctt cacgtcgtgc gccaccgcct ggccaagcca
     4921 tacccacgct cgcggcgcag gcggccccgg cctgaaggct cccgttgtcc acagggatgc
     4981 ggggcgcgag cacttgtccc cagccgtcgg agtcggtctt cagtcagcga gcctccgtgg
     5041 gcacgctggc gccatggatg atgcacgagg tctaccgaac acgtgggtgc ccgcccttcg
     5101 ccgcgcccga acgttcctca ccgtgcagga ggaggtgggg ttggctctgc gcgagcatcg
     5161 ccggcgactc ggcctgagtc agcgtgcgta cgcgaagcat cggggtctga gtcgcgccat
     5221 gctcgctcga ctcgaagcag gcgccgggca gatgcgcctc gacacgatct ccgatgctct
     5281 cgtcggaacg ggcttcatgc tgtgtgtcac cttctcccag agcgagcctg atccgtcccc
     5341 ggacgacgcg gcatcgggat gcgccgccgc cagcgacgat gagccgcaga tccaggtggt
     5401 gcctcggcgg gtccctcctg aggcctggtc gccgaccgac ctcgtggccg cggttcgcgg
     5461 tggtggccgc cggttcccgg ctcaccgaaa ggtctacgcg gtgaccaacc cgccgctgtg
     5521 gtggtggatg cacgagttct tcaacggtcc gaccgagaag ccgaagtggt acgcacccgt
     5581 gccacgtccc gacctcactc tgctcctcgg cgacctgggt gacgacgacg agggggatga
     5641 ccacggggac agcagccccg acgacgccgc ggcggctcca cacggcgacg aacacgacgc
     5701 ggcgtgatcg acagctcgcg caaccctcac agccacccaa ccatccctgt ccgcccctct
     5761 gggacatccc tgccctcccc tgcgaaccat cccccggatg gttcgccggc gcggagcatc
     5821 ccttgggtgg cgcactactc gatccatccc ctggatggtt cgagcacggg gcgagcgggg
     5881 cccgtctggc gtgccacgcg atgcatcccg ggattgcttc gcgtggtgag ccggcggggc
     5941 aggtggctca cgatgtgaag catccggacg tccccccacc agtacctggc cccgctctca
     6001 gcggtcgccg ttgcggatgc cgccccaccg gtgtcagcgc accaccttca caggctgtgg
     6061 ccgacgaaca ccggctcgtt gacgagggtg acgccgaaga cgtcgtgaac gccgtcgcgc
     6121 accgttcgcc ccaagtcagc gatctcgtat gccgtcttct gc
//
LOCUS       DAOSOC010000149         6162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_12836_length_6162_cov_8.492877, whole genome shotgun sequence.
ACCESSION   DAOSOC010000149 DAOSOC010000000
VERSION     DAOSOC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12836_length_6162_cov_8.492877"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PL014_10435"
     CDS             <1..1186
                     /locus_tag="PL014_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT57551.1"
                     /translation="DLEDSSAELSNAYIALQQTKAKLPAAQTALAEAESAALAADRAN
                     TLAAQELEAARASEAKAQAELGSTTKEVTAGRSRVAQFAAQIYQEQGFGQLDMALSST
                     DPQQFADRIALVDTVMDVQGETLDRLATEKASLTALEDHLSALRADSAAKKKRAEEAL
                     AAAESARDSAARAKADLDALAAQQTSQAAEVESQIAADKVRLSAMKAEQSRLKAVLVA
                     RAAEARRKAAAAAKAEAARRAAEKKAGLAARPSTGSGSSGSSEGDDGGYLSRPITGRI
                     SSEFGMRFHPIDRVWRLHAGRDFAAPCGTPVRAAAPGEIIMAGYAAGAGNRIVIDHGI
                     VNGEHLATVYKHLEGYAQRSGKVSRGEVIGYEGSTGLSTGCHLHFETHENGEPVDPRR
                     WL"
     gene            1247..1726
                     /gene="smpB"
                     /locus_tag="PL014_10440"
     CDS             1247..1726
                     /gene="smpB"
                     /locus_tag="PL014_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOT57552.1"
                     /translation="MAKETGRKLVASNRKARHDYLIEDTYEAGLVLMGTEVKALRMGR
                     ASLVDGWAGFDRDDELWLEGVHIPEYFNGTWNNHTPRRRRKLLLHRRELNKILQATRE
                     TGHTIVPLSLYFKDGRAKVELAVAKGKKNYDKRHALRERQDNREAARAMSARTRGED"
     gene            1728..>3604
                     /locus_tag="PL014_10445"
     CDS             1728..>3604
                     /locus_tag="PL014_10445"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOT57553.1"
                     /translation="MDSPVAPVRRSATPWVGFWACLGLIVAAVVLAPLALAAGERMPA
                     LQAWGPALVLPTEEQADSLSTQFEVQSDGSVIVTETIRWRFPDGEDRHGILRNVKVRA
                     GYQDSETQYRYYELSGVVVTSPSGAPTDIAISDMGAFRQIRIGSPSQTVRGTADYVVQ
                     FRLANLVNDIGDGTAEFYYNVVDPSNGFPQLGVRAAVTAPVPATRAACFYGELGADTP
                     CQAQAGATSTFSSPDLAAREGMSVVTSYPRDAFGDLTPDLRDGDSEAGAGSPVSPGQS
                     RALGWLAMGLGVLGPLLAGSLMGMLVWTRGRDEQYAGLTPGLTPGPGEDVPVVVGGTA
                     PTVAVQFNPPPGVQPGMVGTIVDEQVNLVDVTATLVDLAVRGHLTIARDDRGIFRADD
                     WVLTRTAPPASAVPLAPYEQVLIDSIFAGGNQIALSQLKNRFKPTLDAVERLMYEEVV
                     ERGWFRRSPERARAGWTGLGTLLIFGSVFAAFFLGGMLSGLFADSGFPISPTWVLVGG
                     GVVSGLIVRALGQRMAARTASGSAMLAQSRGFERYIATAEANQIKWEEAQEVFSKFLP
                     FAIVFGLADRWAKTFEEVAAAAAAAGVSVASPTWYVGNWGTGGFSDVASSMDSFSTVA
                     AG"
     assembly_gap    3605..3704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3768..3941)
                     /locus_tag="PL014_10450"
     CDS             complement(3768..3941)
                     /locus_tag="PL014_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HOT57554.1"
                     /translation="MGIGDKIENAKDELVGKGKEAAGKVTDNERLEAEGRAQQAEADV
                     KQAGEKVKDAFKN"
     gene            complement(4066..>6162)
                     /locus_tag="PL014_10455"
     CDS             complement(4066..>6162)
                     /locus_tag="PL014_10455"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOT57555.1"
                     /translation="ADPVFQAGEATTSFIDQRPELLEARMGGDRGTRLLTYLADVSVN
                     QPHGPATTHLKPRTKLPTLDTASPLPRGSRDLLREVGPAQFARQLRERTDVPVTDTTF
                     RDAHQSLLATRMRTRDLLHVAGHVSRLTPELLSMEAWGGATYDVALRFLSEDPWERLA
                     LLREAMPNIPLQMLLRGRNTVGYTPYPTKVTDAFVAEAASTGLDIFRIFDSLNDVSQM
                     RPAVDAVLATDTAVAEVALCYTGNLLDPGERLYTLDYYLRLAEQIVDTGAHVLAIKDM
                     AGLLRAPAAATLVRALRERFDLPVHLHTHDTAGGQIGTLLAAIDAGVDAVDAACSSMS
                     GTTSQPSLSALVAATDGTERPTGLDIDKVFSLEPYWEAVRTIYKPFESGLASPTGRVY
                     FHEIPGGQLSNLRQQAIALGLGDRFEDIEDMYAAANRILGNIVKVTPSSKVVGDLALA
                     LVGAGADPADFEENPTRYDIPDSVIGFLEGELGDPPGGWPEPFRTKALQGRAAKPRQT
                     ELTPEQESALATEPRRTLNQLLFPGPTREFEASREAYGDLSVLGTIEFLHGLEQGTEY
                     EAEIEAGKRLILGISSVGEADRQGMRTVMCTLNGQFRPVTVRDRSITADVKAVEKADP
                     SVPGHVPAPFSGVVTPVVAEGDTVEPGGVVATIEAMKMEANITSPVGGTVARLAFSGH
                     HQQVEGGDLIVVVTPA"
BASE COUNT          876 a         2108 c         2162 g          916 t
ORIGIN      
        1 agacctcgag gattcctcgg ctgagctctc gaacgcgtac atcgcgctcc agcagaccaa
       61 ggcgaagctg cccgccgccc agaccgccct ggccgaggcc gagtcggccg ccctcgccgc
      121 agaccgggcc aacacgctgg ctgcccagga gctcgaagcc gcccgggcca gcgaggccaa
      181 ggcacaggcc gaactcgggt cgacgaccaa ggaggtcacg gccgggcggt cccgggtggc
      241 ccagttcgcc gcgcagatct accaggagca gggtttcggc cagctcgaca tggcgctgtc
      301 cagcaccgac cctcagcagt tcgccgaccg gatcgccctc gtcgacacgg tgatggacgt
      361 gcagggcgag acccttgatc gcctcgcgac cgagaaggcc agcctgaccg cgctcgagga
      421 ccacctctcg gcgctgcgcg ccgactcggc cgcgaagaag aagcgtgccg aggaagccct
      481 cgcggccgcc gagagcgctc gcgactccgc ggcccgcgcc aaggccgacc tcgacgccct
      541 cgccgcccag cagaccagcc aggccgccga ggtcgagtcg cagatcgctg ccgacaaggt
      601 tcgcctcagc gccatgaagg ccgagcagag ccggctcaag gccgtcctcg tggctcgcgc
      661 cgccgaggcg cggcgcaagg cggcagccgc agccaaggca gaagccgctc ggcgggccgc
      721 ggagaagaag gccgggctgg ccgcccggcc gtcaaccgga tccggctcct ccggctcctc
      781 cgagggtgac gacggtggct acctcagccg cccgatcacc ggccggatca gttccgagtt
      841 cggcatgcgc ttccacccca tcgaccgcgt ctggcggctg cacgccggcc gcgacttcgc
      901 cgcaccgtgc gggacgcccg ttcgcgctgc tgccccgggc gagatcatca tggccggcta
      961 cgccgcgggc gccggcaacc gaatcgtgat cgaccacggg atcgtcaacg gcgagcacct
     1021 cgcgacggtg tacaagcacc tggagggcta cgcccagcgc agcggcaagg tctcgcgcgg
     1081 cgaggtcatc ggttatgagg gcagcaccgg cctctccacc ggttgtcacc tgcacttcga
     1141 gacccacgag aacggcgagc ctgtcgaccc gcgtcgctgg ctgtgacaac ccgggcttcg
     1201 ggtaatgggt gcggtctgcg ccctcgcgga aggtaacctc gctcccgtgg ccaaggagac
     1261 cggacgaaag ctcgtcgcga gcaaccgcaa ggcccgtcac gactacctca tcgaggacac
     1321 gtacgaggcg ggcctcgtcc tgatgggcac ggaggtcaag gcgctgcgga tggggcgcgc
     1381 ctcgctcgtc gacggatggg cgggctttga ccgtgacgac gagctgtggc tcgagggtgt
     1441 tcacatcccc gagtacttca acgggacgtg gaacaaccac acgccgcgcc gccgccgcaa
     1501 gctgctgctc caccggcgtg agctgaacaa gatcctccag gccacccgcg agaccgggca
     1561 caccatcgtg ccgctctcgc tgtacttcaa ggacggccgg gccaaggtcg agctcgccgt
     1621 ggccaagggc aagaagaact acgacaagcg ccatgccctg cgcgaacgcc aggacaaccg
     1681 cgaggcagcc cgcgccatgt cagcccgcac ccgcggcgag gactgaggtg gacagcccgg
     1741 tcgcaccggt gcgtcgatcc gcgacgccgt gggtgggatt ctgggcctgc ctcgggctga
     1801 tcgtcgcggc ggtggtgctc gcaccgctgg cgctggctgc gggggagcgg atgccggcgc
     1861 tgcaggcctg gggcccggcg ctggtgctgc ccaccgagga gcaggccgac tccctgtcaa
     1921 cgcagttcga ggtccagtca gacggatcgg tcatcgtcac cgagacgatc cggtggcgct
     1981 tccccgacgg cgaggatcgc cacggcatcc tgcgcaacgt caaggtccgc gcgggctacc
     2041 aggacagcga gacccagtac cgctactacg agctgtccgg ggtggtggtc acctcaccgt
     2101 ccggggcgcc gaccgacatc gcgatctccg acatgggcgc cttccgccag atccggatcg
     2161 gcagcccctc gcagaccgtg cgcggcaccg ccgactacgt cgtgcagttc cggttggcga
     2221 acctggtcaa cgacatcggt gacggcacgg ccgagttcta ctacaacgtc gtcgacccct
     2281 cgaacggctt cccgcagctg ggcgttcgcg ccgcggtgac ggcgcccgtc ccggccacca
     2341 gggccgcctg cttctacggc gagctcggcg ccgacacacc ctgccaggca caggcggggg
     2401 ccacctcgac gttcagttca cccgacctcg cggcccgtga gggcatgagc gtggtgacgt
     2461 cgtacccgcg cgacgccttc ggcgacctca ccccggacct tcgcgacggt gactccgagg
     2521 cgggcgccgg atcaccggtc agccccgggc agtcgcgcgc tctgggctgg ttggccatgg
     2581 gcttgggcgt gctcgggccg ctgctcgccg gctccctcat gggcatgctc gtgtggacca
     2641 ggggccgcga cgagcagtac gccggcctca ccccgggtct gacccccggg cccggcgagg
     2701 acgtccccgt cgtcgtcggc ggcacggcgc ccaccgtcgc ggtgcagttc aacccgccgc
     2761 cgggtgtgca gcccggcatg gtcggcacca ttgtcgatga gcaggtcaat ctcgtcgacg
     2821 tgacggcgac gctcgtcgac ctcgccgttc gagggcacct gacgatcgcg cgcgatgacc
     2881 gggggatctt ccgggccgac gactgggtgc tcacgcgaac cgcacccccg gcatccgccg
     2941 tgcccctcgc cccgtacgaa caggtgctca tcgactcgat cttcgccggg ggcaaccaga
     3001 tcgccctgtc gcagctgaag aaccgcttca agcccaccct ggatgccgtg gagcgtctga
     3061 tgtacgagga ggtcgtcgag cgtgggtggt tccgtcgaag cccggagcgg gctcgcgcgg
     3121 gctggacggg tcttggcacg ctgctgatct tcggctcggt cttcgccgcg ttcttcctcg
     3181 gggggatgct gtcgggcctg ttcgcggact ccggcttccc gatctcgccg acgtgggtgc
     3241 tcgtcggggg tggggtggtg tccgggctga tcgtgcgagc cctcggccag cggatggcgg
     3301 ctcgaacggc atccggctca gccatgctgg cgcagtcgcg cggtttcgag cggtacatcg
     3361 ccaccgccga ggccaaccag atcaagtggg aggaagccca ggaggtcttc tcgaagttcc
     3421 tgccgtttgc catcgtgttc ggtctggcgg accggtgggc caagaccttc gaggaggtgg
     3481 cggcggctgc ggctgccgcg ggggtgagcg ttgcgagccc cacgtggtac gtggggaact
     3541 ggggcaccgg cggcttctcc gacgtcgcct cgagcatgga ctcgttctcg accgtggctg
     3601 ccggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgtacc cacaccgacc
     3721 aataggtcga tgtgggtacg ggttccttgg ggcgagcccc cggaaggtca gttcttgaac
     3781 gcgtccttga ccttctcgcc ggcctgcttg acgtcggcct cggcctgctg ggcgcggccc
     3841 tcggcctcga ggcgctcgtt gtcggtcacc ttgccggcgg cctccttgcc cttgcccacc
     3901 agctcatcct tggcgttctc gatcttgtcg ccgattccca tggtgttctc cctcgttcga
     3961 tgggtggagc gccggccacg atggtgaccg gtgcgaccct cgacggtacg tacacgctca
     4021 cccaaacggg ggtttgggta cggacccttc gggaaggtcc gggagtcagg ccggggtgac
     4081 gacgacgatg aggtcgccgc cctccacctg ctggtggtgt ccgctgaagg ccaggcgtgc
     4141 cacggtgccg cccacggggc tggtgatgtt ggcctccatc ttcatcgcct cgatcgtcgc
     4201 gacgacaccg ccgggctcga ccgtgtcgcc ctcggcgacg accggcgtga cgaccccgga
     4261 gaacggtgcc ggcacgtgcc cgggcaccga tgggtcggcc ttctccacgg ccttgacgtc
     4321 agcggtgatc gagcggtcgc gcaccgtgac gggtcggaac tgcccgttga gcgtgcacat
     4381 cacggtgcgc atgccctggc ggtcggcctc acccaccgag ctgatcccga ggatcaggcg
     4441 cttgcccgcc tcgatctcgg cctcgtactc ggttccctgc tccaagccgt gcaggaactc
     4501 gatggtgccg agcaccgaca ggtcgccgta ggcctcgcgg ctggcctcga actccctggt
     4561 cggcccggga aagagcagct ggttcagggt gcggcgcggc tcggtggcca gtgcagactc
     4621 ctgctcgggg gtcagctcgg tctgccgcgg cttggcggcg cggccctgaa gtgccttggt
     4681 gcggaagggc tcgggccagc caccgggcgg gtcgccgagc tcaccttcga ggaagccgat
     4741 caccgagtcg gggatgtcgt acctcgtcgg gttctcctcg aagtcggccg ggtcggctcc
     4801 ggccccgacg agggccaggg ccaggtcgcc gacgaccttg gaggacggcg tcaccttgac
     4861 gatgttgccg aggatgcggt tcgccgcggc gtacatgtcc tcgatgtcct cgaagcggtc
     4921 gccgagaccg agcgcgatcg cctgctggcg caggttggac agctgcccgc cggggatctc
     4981 gtggaagtac acgcgcccgg tcggtgaggc cagccctgac tcgaacggct tgtagatcgt
     5041 gcgcacggcc tcccagtagg gctcgagcga gaacaccttg tcgatgtcca gcccggtggg
     5101 ccgctcggtg ccgtcggtgg ccgccacgag ggcggacagg ctcggctggc tggtcgtgcc
     5161 ggacatcgac gaacaggcgg cgtccacggc atccaccccg gcgtcgatgg cggcgagcag
     5221 cgtcccgatc tgaccgccgg cggtgtcgtg ggtgtgcagg tgcacgggca ggtcgaagcg
     5281 ttcacgcagc gcccgcacca gcgtcgccgc ggccggcgcg cgcagcaacc ccgccatgtc
     5341 cttgatggcg agcacgtggg cgccggtgtc gacgatctgc tcggccaggc gcaggtagta
     5401 gtcgagcgtg tagaggcgct caccggggtc gagcaggttg ccggtgtagc acagggccac
     5461 ctcggcgacc gccgtgtcgg tggcgagcac ggcatccacc gcagggcgca tctgcgacac
     5521 gtcgttgagc gagtcgaaga tgcggaagat gtccagaccg gtgctggccg cctccgcgac
     5581 gaacgcgtcg gtgaccttcg tggggtaggg cgtgtagccg acggtgttgc gcccccgcag
     5641 cagcatctgc agagggatgt tgggcatcgc ctcgcgcagc agcgccagcc gctcccacgg
     5701 gtcctcggac aggaagcgca gcgcgacgtc gtaggtcgcg ccgccccatg cctccatgga
     5761 gagcagctca ggcgtcaggc gggacacgtg ccccgcgacg tgcagcaggt cgcgggtgcg
     5821 catgcgggtg gcgagcaggc tctggtgggc gtcgcggaag gtcgtgtcgg ttacgggcac
     5881 gtcggtgcgc tcgcgcagct gccgggcgaa ctgcgccggg cccacctcgc gaagcaggtc
     5941 gcggctgccc cgggggagcg gggatgccgt gtcgagagtc ggcagcttcg tgcggggctt
     6001 caggtgcgtg gtcgcggggc cgtgcggctg gttgacgctc acgtcggcca ggtaggtgag
     6061 caggcgggtg ccgcggtcgc cgcccatgcg cgcctcgagc agctcgggac gctggtcgat
     6121 gaagctcgtc gtcgcctcac cagcctggaa caccgggtcg gc
//
LOCUS       DAOSOC010000150         6098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_13032_length_6098_cov_10.020354, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000150 DAOSOC010000000
VERSION     DAOSOC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13032_length_6098_cov_10.020354"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="PL014_10460"
     CDS             complement(<1..1904)
                     /locus_tag="PL014_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HOT57556.1"
                     /translation="MSNGAYAAAYEQSISDPTTFWGNASSAIEWITEPTTVLDDTNPP
                     FYRWFRGGTLNTCFNALDRHVRDGRGEQAALIYDSPVTSTVETISYAELLTRVATFAG
                     ALDSLGVAKGDRVVIYMPMVPEAVVAMLACARLGAIHSVVFGGFAPAELAARIEDAKP
                     KVIVAASCGIEPSRVVEYKPMLDAALDRSSHTPEAVIVLQREQAAASVGERDTDWEEL
                     DWDELVTDAEPVGCVEVEATDPLYVLYTSGTTGRPKGIVRDNGGHAVALRWSMANVYG
                     IEPGDVWFTASDVGWVVGHSYIVYAPLLTGATTVLYEGKPVGTPDASAFWRVIAQHGV
                     KAMFTAPTAYRAIKREDPTGSLMAGHDLSSLQTVFLAGERLDPDTYEWASQVLGVPIV
                     DNWWQTETGWPIAANLRGLEPMPIKAGSPSVRVPGYDVQVLDDAGQPMPPGHEGAIAI
                     KLPLPPGTLPTLWEDDERYVAGYLSVYEGYYLTGDGGMIDADGYLYVMGRTDDVLNVA
                     GHRLSTGSIEAALAGHPAVAECAVIGVADDFKGQVPRGLVVLKGGVDGAGDEGVRICK
                     ELVQRVRDEVGAVASLKQVDIVAGLPKTRSGKILRKTMRELADGKVPTVPGTIEDASV
                     LDALGPVLRPG"
     gene            complement(2066..2153)
                     /locus_tag="PL014_10465"
     tRNA            complement(2066..2153)
                     /locus_tag="PL014_10465"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2117..2119),aa:Ser,seq:gga)
     gene            2215..3147
                     /locus_tag="PL014_10470"
     CDS             2215..3147
                     /locus_tag="PL014_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57557.1"
                     /translation="MSTGGMSGSDWNAIVKGGDPPSVTQSAPWLAGIRLTLAQVPVRQ
                     VLGAAAVLVWAVWLIAMWVTQPRIVPPSQLEADLAQGQVTSFGVVRLVETDGGFFISG
                     GRTSVEPAGDGRDALGETVDEWPSSDGRLTVAYWVDSPVADLRVIDPDRAAPPELEAI
                     ADRLGAAAVEDRTRSLTYSTFSDGVSKADVAISLVALLVVVMGPRPGRGTRWFWFWLI
                     WLPFGLGLLAFAVFEVLRPPRLVLVPESAGSGALSDEGDPTASDDDPASAHPVSPRRY
                     SGWAGLGLSILGGILIGTIADQLAQTLPLVFVRY"
     gene            complement(3160..3627)
                     /locus_tag="PL014_10475"
     CDS             complement(3160..3627)
                     /locus_tag="PL014_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HOT57558.1"
                     /translation="MTPRTLFTWLARAEVVTWTLLLIGMALKYVTRTTELGVSVFGLV
                     HGVVFLAFVLVTVVLWVDQRWPLREVVLVLACAIPPFATLYAERRVERLQLAPSRWRL
                     RAGGDEAASVPERAVAAALARPVAAALVGALAVGTATLVLLLIGPPPVPGNGA"
     gene            3684..3780
                     /gene="ffs"
                     /locus_tag="PL014_10480"
     ncRNA           3684..3780
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PL014_10480"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3776..4558)
                     /locus_tag="PL014_10485"
     CDS             complement(3776..4558)
                     /locus_tag="PL014_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOT57559.1"
                     /translation="MRIDVWSDILCPFCHLGRRHLERALEQFEHRDEVGVVWHSFQLD
                     RTADPVDDAPVIDRVAAKYGVPREQMVAQHEQMARDAASVGLDFQWEKLRGGNSYDAH
                     RLIHFARTLGLEEPLTQRLMTAWYSEGAAIGDQDTLVRLAVEAGLEADAVRALLASDD
                     HGIDVRTDLALASQIGITSVPTFVLDQKYGVTGAQPVEVLLNSIRQVWDLQGTEPEPV
                     ADAGGCGGGCCGGVCGSDSADAGATADGTEATESPAADPARA"
     gene            4646..>6098
                     /locus_tag="PL014_10490"
     CDS             4646..>6098
                     /locus_tag="PL014_10490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HOT57560.1"
                     /translation="MSGIAARVRQVSTALYRRYRPETFADVIGQEHVTEPLMQALRTG
                     RVNHAYLFSGPRGCGKTTSARILARCLNCEQGPTATPCGTCDSCVALARGGSGTVDVI
                     EIDAASHGGVDDARDLRERASYGPAQSRYKIYIIDEAHMVTPQGFNALLKIVEEPPEH
                     VKFVFATTEPEKVIGTIRSRTHHYPFRLVPPARLHSYMEELCVREGVAVAPGVLSFVV
                     RAGGGSVRDSLSVLDQLMAGSGEAGLTYESAAALLGFTDGELLDGAIDAFAAGDAASV
                     FRTIDRVVETGLDPRRFVEDVLERLRDLIVVAAVGEGAGQVLRHVPQDQLERMMVQAA
                     AFGSGALSRSADVVNAGLTEMTGATAPRLQLELIAARVLLPGAAGEAGYAARLDRLER
                     RLDVGGVPTGAVPYSAPAPAAAPAPVAEPAPAPAPAPAAAPAPAPAAAPPPDPAPASA
                     PPSAGVEVMTHGSAGGSTSTPAQQAAPAQQAAPA"
BASE COUNT          854 a         2134 c         2212 g          898 t
ORIGIN      
        1 ccggggcgca gcaccggtcc gagcgcgtcg agcaccgacg cgtcctcgat ggtcccgggc
       61 accgtgggca ccttgccgtc ggccagctca cgcatcgtct tgcgcaggat cttgcccgag
      121 cgggtcttcg gcagccccgc cacgatgtcg acctgcttca ggctcgccac ggcaccgacc
      181 tcgtcgcgca cccgctgcac gagttccttg cagatgcgca caccctcgtc gccagcaccg
      241 tcgacgccac ccttgagcac gacgagccca cggggcacct ggcccttgaa gtcgtcagcc
      301 acgccgatga ccgcgcactc ggcgacggct gggtgcccgg ccagggcggc ctcgatcgag
      361 ccggtcgaca gccggtgccc ggcgacgttc agcacgtcgt cggtgcggcc catgacgtag
      421 aggtagccgt cggcgtcgat catcccgccg tcaccggtga ggtagtaccc ctcgtacacc
      481 gagaggtagc cggcgacgta gcgctcgtcg tcctcccaca gggtcggcag ggtgccgggc
      541 ggaagcggca gcttgatggc gatggcgccc tcgtggccgg gcggcatcgg ctggccggcg
      601 tcgtcgagca cctgcacgtc gtagccgggc acccgcaccg agggcgagcc cgccttgatc
      661 ggcatcggct ccagaccgcg caggttcgcc gcgatcggcc agccggtctc ggtctgccac
      721 cagttgtcga cgatcggcac gccgagcacc tggctggccc actcgtaggt gtcggggtcg
      781 agccgctcgc ccgcgaggaa cacggtctgc aggctcgaca ggtcgtgacc ggccatgagg
      841 gacccggtcg ggtcctcgcg cttgatggcc cggtaggcgg tcggcgcggt gaacatcgcc
      901 ttcaccccgt gctgggcgat gacccgccag aaggcgctcg cgtcgggggt gcccacgggc
      961 ttgccctcgt agagcaccgt cgtcgcgccc gtcagcagcg gcgcgtagac gatgtaggag
     1021 tggccgacga cccacccgac gtcggatgcc gtgaaccaca cgtcccccgg ctcgatgccg
     1081 tagacgttgg ccatcgacca ccgcagcgcc acggcgtggc cgccgttgtc gcgcacgatg
     1141 cccttggggc gcccggtcgt gcccgaggtg tagagcacgt agagcgggtc ggtcgcctcg
     1201 acctcgacac agccgaccgg ttcggcgtcg gtgacgagct cgtcccagtc cagctcctcc
     1261 cagtcggtgt cgcgctcgcc gaccgaggcc gcggcctgct cacgttgcag gacgatgacg
     1321 gcctcggggg tgtggctgct gcggtcgagc gcggcatcca gcatcggctt gtactccacg
     1381 acccggctgg gttcgatgcc gcaggatgcc gcgacgatca ccttgggctt ggcgtcctcg
     1441 atccgggcgg cgagctcggc cggggcgaac ccgccgaaga ccaccgagtg gatcgctccg
     1501 agccgggcgc acgcgagcat cgcgacgacg gcctcgggca ccatcggcat gtagatgacg
     1561 acccggtcgc ccttggccac cccgagggag tccagcgcgc cggcgaacgt cgccaccctg
     1621 gtgagcagtt cggcgtagct gatcgtctcg accgtcgagg tgaccgggct gtcgtagatc
     1681 agcgcggcct gctcgccgcg gccgtcgcgc acgtgccggt cgagggcgtt gaagcaggtg
     1741 ttgagggtgc cgccacggaa ccatcggtag aacggcgggt tggtgtcgtc gagcaccgtc
     1801 gtgggctcgg tgatccactc gatggcgctg gaggcgttgc cccagaacgt cgtcgggtcg
     1861 gagatcgact gctcgtatgc cgctgcgtag gcgccgttgc tcatggaacc tcatcgtgct
     1921 ggggtcgctc gctcggcgcc accggtgcgg taggaaccgt ccgtggacgg gcggttggcg
     1981 cgacgagcgg atgccagact gcccggacgg agattccacc gcacacggaa cggccccgga
     2041 cgcaagcgcc cggggccgtt ccgtgtggcg gaggatgggg gatttgaacc cccgagggtt
     2101 ttatcccaac acgatttcca atcgtgcgca ctaggccact atgcgaatcc tccgcgggcg
     2161 aggctactag agggaacgct cgccccgaaa tcgcggcggt gcgatgatgg caccgtgagc
     2221 actgggggaa tgtcgggaag cgactggaac gccatcgtca aggggggtga tccgccgtcg
     2281 gtgacgcaga gcgcgccgtg gctcgccggc atccgactga ccctcgcgca ggtgcccgtc
     2341 aggcaggtgc tcggtgcggc agccgtgctg gtctgggccg tatggctgat cgcgatgtgg
     2401 gtcacccaac cgcgcatcgt gccgcccagt cagctcgagg ccgacctggc gcagggccag
     2461 gtcacgagct tcggggtggt gcgcctcgtc gagaccgacg ggggtttctt catctccggc
     2521 ggcaggacct cggtcgaacc ggccggtgac ggccgcgacg cgctgggcga gacggtggac
     2581 gagtggccgt cgagtgacgg ccggctcacg gtcgcctact gggtggactc cccggtcgcg
     2641 gacctgcggg tgatcgatcc ggatcgggcc gcgccaccgg agctcgaggc catcgccgac
     2701 cgcctcggcg ccgccgccgt cgaggaccgc acccggtcgc tgacctactc cacgttctcc
     2761 gacggggtca gcaaggcaga cgtggccatc agcctcgtcg ccctgctcgt cgtcgtcatg
     2821 ggcccgaggc ccggccgcgg cacccggtgg ttctggttct ggctcatctg gctcccgttc
     2881 ggcctgggcc tgctcgcctt tgccgtgttc gaggtcctca ggccaccgcg gctggtgctc
     2941 gtccccgaat cggccgggag cggtgcactg agcgacgaag gcgacccgac ggcatccgat
     3001 gacgacccgg catccgcgca ccccgtctcg ccacgcaggt acagcggttg ggcagggctc
     3061 gggctctcga tcctcggggg gatcctcatc ggcacgatcg ccgaccaact ggcgcagacg
     3121 ctgccgctgg tcttcgtgcg gtactgagcc cgcgtgcgct caggcgccgt tgcccggcac
     3181 cggcggcggg ccgatgagca ggagcacgag cgtcgcggtc cccacggcga gcgccccgac
     3241 gagagccgcg gcaaccggcc gggcgagggc ggccgcgacc gcacgctcgg gcaccgatgc
     3301 cgcctcgtca ccgccggccc gcagccgcca gcggctcggc gcgagctgga ggcgctcgac
     3361 ccgccgctcg gcgtacagcg tcgcgaacgg ggggatggcg caggcgagca cgagcacgac
     3421 ctcgcgaagc ggccagcgct ggtcgaccca cagcacgacc gtcacgagaa cgaacgcgag
     3481 gaacaccacg ccgtgcacca acccgaacac gctgacaccg agctcggtcg tgcgggtcac
     3541 gtacttcagc gccatgccga tgagcagcag cgtccaggtc accacctcgg cgcgagcgag
     3601 ccaggtgaac agcgttcggg gggtcatgtc gtccttctca tcgggcgatt tagtcggatg
     3661 ccgtcagggc gcctaagatt gccaacggca ccccgcgtgg tggtaccccg ccgaactccc
     3721 ccagggcagg aatgcagcaa gggcaggtgg gctcttccag gtacgcgggg tgtccttatg
     3781 cccgggcggg gtcggccgcg ggtgactcgg tggcctcggt gccgtcggcg gttgcacccg
     3841 cgtcagcgga gtcgctgccg cacacaccac cgcaacaccc gcccccgcag ccacccgcgt
     3901 cggccaccgg ttcgggctcg gtgccctgca ggtcccagac ctgccggatg gagttgagca
     3961 gcacctcgac gggctgggcg ccggtgacgc cgtacttctg gtcgaggacg aacgtcggca
     4021 ccgaggtgat gccgatctgc gaggccaggg cgaggtcggt gcgcacgtcg atgccgtggt
     4081 cgtcgctcgc gaggagggcg cggacggcat ccgcctcgag accggcctcg acggccaggc
     4141 gcacgagggt gtcctggtcg ccgatggccg cgccctcgga gtaccaggcg gtcatgaggc
     4201 gctgcgtgag cggttcctcg aggccgagcg tgcgggcgaa gtggatgagg cggtgggcgt
     4261 cgtaggagtt gccgccgcgc agcttctccc actggaagtc aaggccgacg gatgccgcgt
     4321 cgcgcgccat ctgctcgtgc tgggccacca tctgctcgcg cgggacgccg tacttcgcgg
     4381 cgacccggtc gatcaccgga gcgtcgtcga ccggatcggc ggtgcggtcg agctggaagc
     4441 tgtgccagac cacccccacc tcgtcgcggt gctcgaactg ctcgagggca cgttcgaggt
     4501 gccgcctgcc gaggtggcag aaggggcaga ggatgtcgga ccacacgtcg atgcgcacgt
     4561 ttgtcagggt acgtcgggtg gaggccgaga acccaaccag cacccgcagt gggcggcgtc
     4621 cacaggggac ggcacggcct acgggatgtc tggcatcgcg gctagggttc gacaggtgag
     4681 caccgcgctg taccgccgct accgccccga gacgttcgcc gacgtcatcg ggcaggagca
     4741 cgtgaccgaa ccgctcatgc aggcgctgcg caccggccgg gtcaaccacg cctacctgtt
     4801 cagcgggcct cgtgggtgcg gcaagacgac gagtgcccgc atcctcgccc gctgcctgaa
     4861 ctgcgagcag gggccgaccg cgactccgtg cggcacctgt gactcctgcg tggcgctggc
     4921 ccggggtggg tcaggcacgg tcgacgtcat cgagatcgac gcggcctcgc acggtggtgt
     4981 cgacgacgcc cgcgacctgc gcgagcgtgc gtcctacggc ccggcccaga gccgctacaa
     5041 gatctacatc atcgacgagg cgcacatggt gacgccgcag ggcttcaacg cgctgctcaa
     5101 gatcgtcgag gagccgcccg agcacgtgaa gttcgtcttc gcgacgaccg agcccgagaa
     5161 ggtcatcggc acgatccgct cgcgcacgca ccactacccg ttccggctcg tgccgccagc
     5221 ccgcctgcac agctacatgg aggagctgtg cgtgcgcgag ggcgtggcgg ttgcgccggg
     5281 ggtgctgtcg ttcgtcgtgc gggccggtgg tgggtcggtg cgtgactcgc tctccgtgct
     5341 cgaccagttg atggccgggt cgggcgaagc ggggctgacg tacgagtcgg cggcggcgct
     5401 gctcggcttc accgacggtg aactgctcga cggggcgatc gacgcgttcg cggcggggga
     5461 tgccgcgtcg gtctttcgca cgatcgaccg ggtcgtggag accgggttgg acccgcgacg
     5521 gttcgtcgag gacgtcctgg agcggttgcg cgacctcatc gtcgtggccg cggtcggcga
     5581 gggggcgggg caggtgctcc ggcacgtccc gcaggaccag ctcgagcgga tgatggtgca
     5641 ggcggcggcc ttcggatcgg gggcgctgtc ccgctcggcc gatgtcgtca atgccgggct
     5701 caccgagatg accggggcga cggcgccgcg gttgcagctc gagctcatcg ctgcccgcgt
     5761 gctgcttccg ggggcggcag gggaggcggg ttatgccgct cgcctcgacc ggctcgagcg
     5821 tcgccttgac gtgggtggtg tgccgacggg agcggtcccg tactccgcgc ctgcaccagc
     5881 cgctgcccct gcccccgtgg ccgagcccgc gccagcgccc gcgcccgcgc ctgcggctgc
     5941 accagcccct gcccccgcgg ctgcacctcc ccccgatcct gcaccggcat ccgccccacc
     6001 ctctgccggg gtcgaggtga tgacgcacgg ttccgcgggc ggtagcacct cgactcccgc
     6061 gcagcaggca gcccccgcgc agcaggcagc ccccgcgc
//
LOCUS       DAOSOC010000151         6095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_13042_length_6095_cov_10.773675, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000151 DAOSOC010000000
VERSION     DAOSOC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13042_length_6095_cov_10.773675"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PL014_10495"
     CDS             <1..296
                     /locus_tag="PL014_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HOT57561.1"
                     /translation="GCPNSCARIQVADIGLKGMIVTVDDVQQPGFQVHLGGGLASADR
                     DEAGLGRTVRGLKVTAADLPDLVERLVRRWLDQRAPGETFATWAHRVDEEELR"
     gene            293..1063
                     /locus_tag="PL014_10500"
     CDS             293..1063
                     /locus_tag="PL014_10500"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOT57562.1"
                     /translation="MSADQTTTTTPASTAPTRRRTTDELRALATEGERLLTAAHRPGG
                     TEADAAEVAEWAARTFPGTLAVACSMADAVLPHVVAEQAPGVDVLFLDTGYHFAETYG
                     TRNQVEHELDVTIVDVLPTLTVAEQDAQYGPKLHDRDPAACCRMRKVEPLKAALAGYE
                     AWVTGVRREEGPTRADTPLVTFDEQFGLVKLNPLAAWSFDDLLGYATDRRVPVNLLLS
                     DGYPSIGCEPCTKRVAPGDDPRAGRWAGFAKTECGLHP"
     gene            1060..2010
                     /gene="cysD"
                     /locus_tag="PL014_10505"
     CDS             1060..2010
                     /gene="cysD"
                     /locus_tag="PL014_10505"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOT57563.1"
                     /translation="MSATMTKESTSVSTTSTDLPVLTQLDVLESEAMLVIREITAELE
                     RPVLLFSGGKDSVVMLHVAAKAFWPAPVPFPVLHVDTGHNFPEVLAYRDATVERLGLR
                     LEVASVQDWIDDGRLRERADGTRNPLQTQPLLDAIARHRFDGVFGGGRRDEEKARAKE
                     RVVSLRDDFGQWDPKNQRPELWNIFNPRHRPGEHVRVFPLSNWTELDVWRYIERENID
                     LPGLYYAHDREVFERDGMLLAVGPYSQPRPGERVETRQVRYRTVGDMSCTGAVASDAA
                     SVADVVAEVAASTLTERGATRADDRISEAAMEDRKKEGYF"
     gene            2010..3308
                     /locus_tag="PL014_10510"
     CDS             2010..3308
                     /locus_tag="PL014_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT57564.1"
                     /translation="MSTLLRLATAGSVDDGKSTLVGRLLHDSKSVLVDQLDAVERVSR
                     DRGLDTADLALLTDGLRAEREQGITIDVAYRYFATAERSFVLADCPGHVQYTRNTVTG
                     SSTADALVLLVDARKGVLEQTRRHLAVAALMRVPHVVIAVNKIDLVAHSEEVFRTLAA
                     DVRAIATELGIPDAHAVPVSALVGDNVVDRSPNTPWYDGPSLLELLETLPADTDPVHE
                     SFRMPVQVVIRPQGAARSPEHVEYRGYAGQLASGSVRVGDEVVVHPSGLRSTVAGIDL
                     GEQSLDVAVAPQSVTIRLTDDVDVSRGDLLAAATDPVPLRQEVTALACWLGDEPLRPG
                     ARLLVKHGSRTVLAMVRSIDGRLDLDALRLAPAGELALNDIGRVTVRFAAPLPVEEYS
                     TSRRGGAFLLIDPDDGRTLAAVMADARAPGEHPTNGTTSP"
     gene            3305..4489
                     /gene="cobA"
                     /locus_tag="PL014_10515"
     CDS             3305..4489
                     /gene="cobA"
                     /locus_tag="PL014_10515"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOT57565.1"
                     /translation="MTLPVGLDLTDRRVVVVGGGPAALPGIVALVGAQAAVHVIAPWV
                     CEDAAALVATGEVAWTERDYAGSVDLDGAWLALAASGDVAVDEAVRADAVATRLWCLD
                     ATAALPATARVSTPDGTVDVAVHAGNDAVLARGVARSVGGLLAGGQVDTRRRTRRTGA
                     GWVALVGGGPGDDGLLTVRGRELLASADVVVLDRLAPRAVVDALPADVQVIDVGKSPG
                     RHALPQERINDLLVDLALAGRSVVRLKGGDPYVLGRGGEERLACQAHGIRVEVVPGVS
                     SAMAVPAAAGIPVTHRGVARGFTVVTGHDELPEVPAGTDHTVVLLMGVGRLARSAQAL
                     MDAGRPASCPAAIVERGFSPDQRVTTGTLRDIAERAASVGVENPAVVVVGDVVRLSPD
                     WS"
     gene            complement(4505..5776)
                     /locus_tag="PL014_10520"
     CDS             complement(4505..5776)
                     /locus_tag="PL014_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010533583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOT57566.1"
                     /translation="MIDVLWGLGGMVVLLGLAVAISENRKAIRLRTVGAALALQVAFA
                     VIVLYVPAGRTVLDAITRGVQAVIDSSSAGISFLFGPLIPAEGEGLIFALQVLPVIIF
                     FAALTSVLYHWRILQKVVGWLGTGLRWVLGTSRAESVNAATNIFLGQTESPLVIRPFI
                     ARVSRSSLFAIMVGGLATVAGSVLVGYALLGADLDSLIAAAFMAAPGALLMAKIIVPE
                     SEEVDADGVPVASTDPADTEALEEAEAQAQEEVRHVNVIDAAAAGAADGLKLAANIGA
                     MLLAFISLIALINLILGTVAGWFGVESLTFEQILGWIFAPIMFVIGVPWDEAVRAGSF
                     VGQKTVVNEFVAFSNFGPVADEFTQKTQAIITFALTGFANLGSLAILLGGLGGLAPSR
                     RSEIAQMGVRCVAAATLANLMSAAIAGILIG"
     gene            5871..>6095
                     /locus_tag="PL014_10525"
     CDS             5871..>6095
                     /locus_tag="PL014_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOT57567.1"
                     /translation="MTTIISTLFISLDGVAEIDFAWHGPYFDEAMGECVAADYDGVDV
                     LLLGRGTYESFAGAWPEREAAGGEDAEFGAL"
BASE COUNT          882 a         2303 c         2084 g          826 t
ORIGIN      
        1 acggctgccc caactcgtgc gcccgcatcc aggtcgccga catcggcctc aagggcatga
       61 tcgtgaccgt cgacgacgtc cagcagcccg gcttccaggt gcacctcggc ggcgggctgg
      121 cctcggccga ccgcgacgag gccggcctcg ggcgcaccgt gcgtggcctg aaggtcactg
      181 ctgcagacct acccgacctg gtcgaacgcc tcgtccgccg ctggctggac cagcgcgcac
      241 ccggtgagac gttcgcgacc tgggcccacc gcgtcgacga ggaggagctg cgatgagcgc
      301 agaccagacc acgaccacca ccccggcatc caccgccccg acccgccggc gcaccaccga
      361 cgagttgcgc gcgctcgcca ccgagggtga gcggctcctc accgccgccc accgtccggg
      421 tgggaccgag gcggatgccg cggaggtcgc ggagtgggcc gcgcgcacct tccccggcac
      481 cctcgccgtc gcgtgcagca tggcggatgc cgtgctcccc cacgtcgtcg cggagcaggc
      541 ccccggggtc gacgtgctct tcctcgacac cggttaccac ttcgccgaga cctacggaac
      601 gcgcaaccag gtcgagcacg agctcgacgt caccatcgtc gacgtcctgc ccacgctgac
      661 cgtcgccgag caggacgcgc agtacggccc gaagctgcac gaccgcgacc ccgccgcctg
      721 ctgccggatg cgcaaggtcg aaccgctgaa ggccgcgctg gccggttacg aggcctgggt
      781 caccggcgtg cgacgcgagg agggaccgac ccgggccgac accccgctcg tcaccttcga
      841 cgagcagttc ggcctcgtga agctcaaccc cctcgctgcc tggtcgttcg acgacctgct
      901 cggctacgcc accgaccggc gggtgcccgt gaacctcctg ctctccgacg ggtacccctc
      961 gatcggctgc gagccgtgca cgaagcgcgt cgcccccggc gacgacccac gcgccggacg
     1021 ctgggccggg ttcgccaaga ccgagtgcgg gctgcaccca tgagcgccac gatgacgaag
     1081 gaatccacat ccgtgagcac gacctccacc gacctcccgg tcctgaccca gctcgacgtc
     1141 ctcgagagcg aggcgatgct cgtcatccgc gagatcaccg ccgagctcga acgtcccgtg
     1201 ctgctcttca gcggcggcaa ggactccgtg gtcatgctgc acgtcgccgc caaggcattc
     1261 tggccggccc ccgtgccctt ccccgtgctg cacgtcgaca ccgggcacaa cttccccgag
     1321 gtgctcgcct atcgcgacgc caccgtcgag cggctcggcc tgcggctcga ggtggcctcg
     1381 gtccaggact ggatcgacga cggccgcctg cgcgagcgtg ccgacggcac ccgcaacccg
     1441 ctgcagaccc agccgctgct cgacgccatc gcccggcacc gcttcgacgg ggtcttcggg
     1501 ggcggtcgcc gcgacgagga gaaggcccgc gccaaggagc gcgtcgtcag cctgcgcgac
     1561 gacttcggcc agtgggaccc caagaaccag cgccccgagc tgtggaacat cttcaacccg
     1621 cgccaccgcc cgggcgagca cgtgcgggtg ttcccgctgt cgaactggac cgagctcgac
     1681 gtgtggcgct acatcgagcg cgagaacatc gacctgcccg gcctgtacta cgcccacgac
     1741 cgcgaggtct tcgagcgtga cggcatgttg ctcgcggtcg gtccgtactc ccagcctcga
     1801 cccggcgagc gcgtcgagac ccggcaggtc cgctaccgca ccgtcggtga catgtcctgc
     1861 acgggcgcgg tggccagtga cgcggcatcc gtcgccgacg tcgtcgccga ggtcgcggcc
     1921 tccacgctga ccgagcgcgg ggcgacccgc gccgacgacc ggatctccga ggccgccatg
     1981 gaggaccgca agaaggaagg ctatttctga tgtcgaccct gcttcgcctc gcgaccgccg
     2041 gatcggtcga cgacggcaag tccaccctcg tcgggcggct gctgcacgac tccaagtccg
     2101 tcctcgtcga ccagctggat gccgtggagc gggtcagccg ggaccgcggt ctcgacacgg
     2161 ccgacctcgc gctgctcacc gacgggctac gggccgaacg cgagcagggc atcaccatcg
     2221 acgtcgcgta ccgctacttc gcgaccgccg agcgctcgtt cgtcctggcc gactgtcccg
     2281 gccacgtgca gtacacgcgc aacaccgtca ccgggtcctc gacggccgac gccctcgtgc
     2341 tgctcgtcga cgcccgcaag ggagtgctcg agcagacccg tcgccacctc gccgtcgcgg
     2401 ccctcatgcg ggtgccgcac gtcgtcatcg ccgtgaacaa gatcgacctc gtcgcccaca
     2461 gcgaagaggt cttccgcacc ctggccgccg acgtgcgggc gatcgcgacc gagctcggca
     2521 tccccgacgc gcacgccgtg ccggtctccg ccctcgtcgg ggacaacgtc gtggaccgct
     2581 cccccaacac cccgtggtac gacgggccgt cgctgctcga gctcctcgag accctgcccg
     2641 ccgacaccga cccggtgcac gagtcgttcc ggatgccggt ccaggtcgtc atccgtcccc
     2701 agggcgccgc ccgctcaccc gaacacgtgg agtaccgcgg gtacgcggga cagctcgcat
     2761 cgggttcggt gcgggtcggc gacgaggtcg tcgtgcaccc gtcggggctg cggtccacgg
     2821 ttgcggggat cgatctcggt gagcagtcgc tcgacgtggc cgtagccccg cagtcggtga
     2881 ccatccgcct caccgacgac gtcgacgtca gtcgcggcga cctcctcgcc gccgccaccg
     2941 accccgtgcc gctgcgtcag gaggtgaccg cgctcgcctg ctggctcggc gacgagccgc
     3001 tgcgccccgg tgctcgcctg ctcgtcaagc acgggtcccg cacggtgctc gcgatggtgc
     3061 gctcgatcga cggccgcctc gacctcgacg ccctgcgcct ggccccggcc ggcgaactgg
     3121 ccctcaacga catcggccgg gtgacggtgc gcttcgccgc tcccctgccc gtcgaggagt
     3181 actcgacctc acgccgtggt ggtgccttcc tgctcatcga ccccgacgac ggccgcaccc
     3241 tggccgcggt gatggccgac gcacgcgccc cgggagagca ccccacgaac ggcacgacgt
     3301 caccgtgacc cttcccgtcg ggctcgacct caccgaccgc cgcgtggtcg tcgtcggtgg
     3361 cggccccgcg gcgcttcccg gcatcgtggc cctcgtcggc gcgcaggccg cggtgcacgt
     3421 catcgcgccc tgggtgtgtg aggacgccgc cgccctcgtc gccaccggcg aggtcgcctg
     3481 gaccgagcgc gactacgccg gctccgtcga cctcgacggt gcctggctgg ccctggccgc
     3541 ctcgggggac gtcgccgtcg acgaggccgt gcgggcggat gccgtcgcga cccgcctctg
     3601 gtgcctcgac gccactgcgg ccctgcctgc gacggcccgc gtctcgaccc ccgacggcac
     3661 cgtcgacgtg gccgtgcacg ccgggaacga cgccgtcctg gcccgcggtg tggcccgctc
     3721 ggtggggggc ctgctcgccg gcggtcaggt cgacacccgg cgacgcaccc gtcgcaccgg
     3781 cgccggctgg gtggccctcg tcggtggagg gccgggcgac gacgggctgt tgaccgtgcg
     3841 cgggcgcgaa ctgctcgcga gcgccgacgt cgtcgtgctc gaccgcctcg ctccgcgcgc
     3901 cgtggtcgat gcccttcccg ccgacgtgca ggtcatcgac gtcggcaagt cccccggccg
     3961 ccacgccctc ccccaggagc ggatcaacga cctgctcgtc gacctcgccc tcgccggccg
     4021 gtccgtggtg cgcctcaagg gcggcgaccc ctacgtcctc ggtcggggcg gcgaggaacg
     4081 cctcgcgtgc caggcgcacg gcatccgggt cgaggtcgtg cccggggtga gcagcgccat
     4141 ggctgtgccc gcggcggccg ggatccccgt gacgcaccgg ggcgtggcac gcgggttcac
     4201 cgtcgtcacc ggtcacgacg agctgcccga ggtgccggcc ggcaccgacc acaccgtcgt
     4261 cctgctcatg ggtgtcggtc gcctcgcccg ctcggcacag gcgctcatgg atgccggccg
     4321 cccggcatcg tgccccgccg ccatcgtgga gcgcggcttc tcccccgacc agcgggtcac
     4381 gaccgggacg ctgcgcgaca tcgccgagcg cgcggcatcc gtgggtgtcg agaacccggc
     4441 agtcgtcgtc gtcggagacg tggtgcggct cagccccgac tggagctgag cctctcagcc
     4501 gacgtcagcc gatgaggatg ccggcgatgg cggcactcat gaggttggcc agcgtcgccg
     4561 cagcaacgca ccgcacgccc atctgggcga tctcggagcg ccggctgggg gcgaggccac
     4621 cgagcccacc gaggaggatc gcgagcgagc cgaggttcgc gaacccggtg agggcgaagg
     4681 tgatgatcgc ctgggtcttc tgggtgaact catcggcgac cgggccgaag ttcgagaacg
     4741 cgacgaactc gttgacgacg gtcttctggc cgacgaagct gcccgcgcgc actgcctcgt
     4801 cccacggcac gccgatgacg aacatgatcg gcgcgaagat ccacccgagg atctgctcga
     4861 aggtcagcga ctcgaccccg aaccacccgg cgacggtgcc gaggatgagg ttgatcaggg
     4921 cgatgagcga gatgaaggcc agcagcatcg cgccgatgtt cgcggccagc ttgaggccgt
     4981 cggctgcccc ggcggcagca gcgtcgatga cgttgacgtg ccgcacctcc tcctgcgcct
     5041 gcgcctcggc ctcctcgagc gcctccgtgt cggcagggtc ggtgctggcg acgggaacac
     5101 catcggcgtc gacctcctcg gactcgggga cgatgatctt ggccatgagc agggctccgg
     5161 gggctgccat gaaggcggcc gcgatgagcg agtcgaggtc ggcgccgagc agggcgtagc
     5221 cgacgagcac cgagcccgcg accgtggcga ggccgccgac catgatggcg aacaggctgg
     5281 agcgggagac ccgcgcgatg aaggggcgga tgaccagcgg tgactcggtc tgcccgagga
     5341 agatgttcgt ggcggcgttg accgactcgg cgcgcgaggt gccgagcacc cagcgcagcc
     5401 cggtgccgag ccagccgacg accttctgca ggatccgcca gtggtagagc accgacgtca
     5461 gcgccgcgaa gaagatgatg accggcagca cctggagggc gaagatcagg ccttcaccct
     5521 ccgccgggat gagcgggccg aacaggaagc tgatgccggc actcgaggag tcgatgacgg
     5581 cctgcacccc gcgggtgatc gcgtcgagga cggttcgacc ggcgggaacg tagagcacga
     5641 tgaccgcgaa ggccacctgc agggcgagcg ccgcacccac cgtgcgcagc cgaattgcct
     5701 tgcggttctc cgagatcgcg acggcgagcc cgagcagcac gaccattccg ccgagccccc
     5761 agagcacgtc gatcatccga tcctcctggc ctgacacccg gctcgcgctg agccgctgcg
     5821 ttcaggcagg ctacccagac tgcactcgag aaacggcgaa aggctcctca ttgaccacga
     5881 tcatctccac cctgttcatc agcctcgacg gcgtcgccga gatcgacttc gcctggcacg
     5941 ggccgtactt cgacgaggcg atgggcgagt gcgtcgccgc ggactacgac ggcgtcgacg
     6001 tgctgctgct gggccggggc acctacgagt ccttcgccgg cgcctggccc gagcgggagg
     6061 ccgcgggtgg cgaggacgcc gagttcggcg cgctc
//
LOCUS       DAOSOC010000152         6053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_13185_length_6053_cov_8.147049, whole genome shotgun sequence.
ACCESSION   DAOSOC010000152 DAOSOC010000000
VERSION     DAOSOC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13185_length_6053_cov_8.147049"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..1623)
                     /gene="ccrA"
                     /locus_tag="PL014_10530"
     CDS             complement(283..1623)
                     /gene="ccrA"
                     /locus_tag="PL014_10530"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HOT57568.1"
                     /translation="MDQIREAILSGDRTEQTYAGIEVPKTYRAATVHKDEALMFEGLA
                     SRDKDPRKSLHVEQVPTPELAPGEALVAVMASAINYNTVWTSIFEPVSTFGFLERYGR
                     TSEFAKRHDRDYHVVGSDLAGVVLRVGPGVTKWKPGTEVVAHCLSVELEDAAGHDDTM
                     MDPQQRIWGFETNFGGLAELAIVKANQLMPKPTHLSWEEAASPGLVNSTAYRQLISRN
                     GAHMKLGDRVLIWGASGGLGSYATQMALAGGATPICVVSSPEKAAICRAMGAELIIDR
                     SAEGYRFWNEDGTAQNPKEWQRLGKKIRELTGGHDVDIVFEHPGRETFGASVYVARKG
                     GTIVTCASTSGYMHEYDNRYLWMNLKRIVSSHFANYREAWEANELIDRGLIHPTLSRT
                     YRLEDVGTAALDVHRNLHQGKVGVLTLSPEEGLGVSNPEKRARFESEINRFRNA"
     gene            1775..3085
                     /locus_tag="PL014_10535"
     CDS             1775..3085
                     /locus_tag="PL014_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57569.1"
                     /translation="MSPIALTRRRVLLGGVGLLAYSGTAYWSITERIMRAEVPLYGET
                     VAFGGGGSREIVPVGRVDAVVPGTRVLADRVQTAALLEEEQAWLASCAPWVQTRLDAG
                     DDVLRSALLDLRALSADLKVSVAGWTDRWRYAWPRDVSFVAAALARVGHHDAAADQLR
                     WLRGVQGRDGGFEARYDPSTRRPPDDRVAQLDGTGWVVWGAGQIAAQAPERATELLTP
                     LTSLLVRSCTKLLDSLDEATALPPASSDYWEIVEDTLTLGTAAAVLAGLRSGAVVLPL
                     VGEYALAERARAASDTLAAQVRKHFGPNGYPRLLGGSASDAAVTFLVAPIGPTEADVE
                     VLTAIDRAQTSMLRPAGGLAPGASWKNDGISWTPTTALFAAAWAANGYPSKAEILLKW
                     LGDHRTEAGSYPEKVLHDGRPAAVAPLAWTASLVVIATHELVRG"
     gene            3082..5133
                     /locus_tag="PL014_10540"
     CDS             3082..5133
                     /locus_tag="PL014_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57570.1"
                     /translation="MMRRALVAAMALVAATLGLAATPWAWATASAATSATTATASNTP
                     PLVIVGAPGLAWSDLERDDLPALTALVEQGASGSLTVRAVRSRSCAIDGWLTLSAGRR
                     AGDLPGPCREPLPPVGGLVPYWSDYLAAAAADSYDARPGLLAEAVAASGACLQSSGAG
                     AAIGAADPDGSVGSTYSDTVPTSVECPVVLVDGGALPVTQPERADAVARLDALVAQVV
                     GAAPGADVVVAGLGDGDSSVRPRAVVASGPSFGEGLLTSGSTRQPGAIQLQDLTATAL
                     ERVGVEDAPISGRAVSVVSGASSAGQRVDGLVGFETRAATLRSVSPQVTAWLAVGFAL
                     WAALGAWSWWRRGASRELPRALVVGGVAVAAVPISTFAANLVPWWQMPAPAAVFVAIL
                     VAVTALVAAVALGAERRHPLGALRTLALFTVVVLAGDVLLGSRLQLGSVFGQNPTVGG
                     RFYGFGNTSFALYGLAALVLVAWVARARWRSRQVAVGLGLGLLVVLLAIEGLPSLGAD
                     FGGPPGLLLGGLVVIAGVAGVRLTLRRSVVAVVAAGLLVGAISWLDWRRPQASRTHLG
                     EFVDTVISGEAGSVLGRKLAQNLTNLGSPPLLAISIATVVLVVVAWRVGWRPVDAGVP
                     VLRGAVVLALVGFAVNDSGLVIPAFIAVVLAPTLVAAGAASDGRGGSQNAGSAEPGVP
                     R"
     gene            5138..5395
                     /locus_tag="PL014_10545"
     CDS             5138..5395
                     /locus_tag="PL014_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57571.1"
                     /translation="MSNTMPGSGPWRPTSYVGPLVMLLMGVGFLALGWWANAQAEVVR
                     ATDVWAYNSLAAVALLTGALFLPFAGATFVRAMRNRKALVR"
     gene            complement(5429..5869)
                     /gene="mce"
                     /locus_tag="PL014_10550"
     CDS             complement(5429..5869)
                     /gene="mce"
                     /locus_tag="PL014_10550"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOT57572.1"
                     /translation="MTALPTDLFTAIDHVGVAVPDLDEAIAFYTSTYGMRMLHEETNE
                     EQGVREAMMAVGDSGSCIQLLAPLTPASTIAKFLDRSGPGIQQLAYRVTDLDAVSATL
                     RERGLRLLYDAPRRGTSNSRVNFIHPRDAGGILVELVEPADSAH"
BASE COUNT          703 a         2096 c         2283 g          971 t
ORIGIN      
        1 ggcggtgtgg tcgacgtcgg cgggttcggc gtccgtcgcc cgggccgcga tgcggttgct
       61 ctcctcgagc gccgcgagtt gccgtcgccg gaactcaccc caccgggtca ccgggctcca
      121 cagcctcgac acgtccacgc cacacctccc gcggcaaccc tacggtgaga tgaggccgcc
      181 gggcgccacg gcatccctct ccgacttcgt gcgacttcgg caccggtctg ggcaggtgtg
      241 tggcgcagaa ccgcacccaa gtcgcacgaa gtcgggaaaa ggtcaggcgt tgcggaagcg
      301 gttgatctcg ctctcgaagc gggcccgctt ctcggggttg ctcacgccga ggccctcctc
      361 gggcgacagg gtgagcacac cgaccttgcc ctggtggagg ttgcggtgca cgtccagggc
      421 ggcggtaccg acgtcctcga ggcggtaggt gcggctgagg gtcgggtgga tcaggccgcg
      481 gtcgatgagc tcgttggcct cccacgcctc gcggtagttc gcgaagtgcg agctgacgat
      541 gcgcttgagg ttcatccaca ggtagcggtt gtcgtactcg tgcatgtacc ccgaggtcga
      601 cgcacacgtg acgatcgtgc cgcccttgcg ggcgacgtag acgctggcgc cgaaggtctc
      661 gcggccgggg tgctcgaaga cgatgtcgac gtcgtgccca ccggtcagct cacggatctt
      721 cttgcccagg cgctgccact ccttggggtt ctgcgccgtc ccgtcctcgt tccagaaccg
      781 gtacccctcc gcggagcggt cgatgatgag ctcggcgccc atcgcgcggc agatcgcggc
      841 cttctcgggg gaggagacca cgcagatcgg ggtcgcgccg ccggccaggg ccatctgggt
      901 cgcgtaggag cccaatccgc ccgacgcacc ccagatgagc acgcggtcac cgagcttcat
      961 gtgggcgccg ttgcgcgaga tgagctggcg gtaggccgtg ctgttgacca ggccgggaga
     1021 tgccgcctcc tcccacgaca ggtgggtggg cttgggcatc agctggttgg ccttgacgat
     1081 cgcgagctcg gccagaccgc cgaagttggt ctcgaacccc cagatccgct gctgcgggtc
     1141 catcatcgtg tcgtcgtgac cggcggcgtc ctcgagctcg acggacaggc agtgcgcgac
     1201 gacctcggtg ccgggcttcc acttggtcac acccggaccc acgcgcagga cgacgcccgc
     1261 gaggtcggag ccgacgacgt ggtagtcgcg gtcgtgacgc ttggcgaact ccgaggtgcg
     1321 gccgtagcgc tcgaggaacc cgaacgtcga caccggctcg aagatcgagg tccacacggt
     1381 gttgtagttg atggcgctgg ccatgacggc gacgagggct tcaccgggcg cgagttcggg
     1441 ggtgggcacc tgctcgacgt gcagggactt gcgcgggtcc ttgtcgcggc tggccagacc
     1501 ctcgaacatc agggcctcgt ccttgtgcac ggtcgcggcc cgataggtct tcggcacctc
     1561 gatcccggcg taggtctgct cggtgcggtc cccggagagg atggcctcgc ggatctggtc
     1621 catgcggtcg tgctcctttg gcggcgaagg ctgggtcttg cgtgaaccta gccgccgacc
     1681 cggccgcggt cgcgtgcgta tgacggtgcc cacagcgtga tccggctcac gggggtcggg
     1741 agccctgctc gggcatccgg catacagttt cgtcgtgtcc ccgatcgccc tgacccgccg
     1801 ccgcgtgctg ctcggtgggg tcgggctgct ggcctactcg ggcacggcgt actggagcat
     1861 caccgagcgg atcatgcgcg ccgaggtgcc gttgtacggg gagacggtgg ccttcggcgg
     1921 gggcgggagc cgtgagatcg tcccggtggg acgggtggat gccgtggtgc cgggcacgcg
     1981 ggtactcgcc gaccgcgtcc agacggctgc cctgctcgag gaggagcagg cgtggctcgc
     2041 ctcgtgcgcc ccgtgggtgc agacccgact cgacgcgggt gacgacgtgc tccgctctgc
     2101 cctgctcgac ctgcgcgccc tctccgccga cctcaaggtc tccgtggcgg ggtggaccga
     2161 ccggtggcgc tacgcgtggc ccagggacgt ctcgttcgtg gcagcagccc tggcccgggt
     2221 cggccaccac gacgcagcgg ccgaccagct gcgctggctg cgcggtgtcc agggcaggga
     2281 cggcgggttc gaggcgcgct acgacccgag cacgcggcgc ccccctgacg accgcgtcgc
     2341 ccagctcgac ggcaccggct gggtggtgtg gggcgctggc cagatcgcgg cgcaggcccc
     2401 tgagcgggcc accgagctgc tcaccccgct cacctccctg ctcgtgcgca gctgcaccaa
     2461 gctgctggac tccctcgacg aggcgaccgc cctgccgccg gcgtcgtcgg actactggga
     2521 gatcgtcgag gacaccctca ccctgggcac cgcggccgcc gtcctcgccg ggctgcgcag
     2581 cggcgcggtc gtgctgcccc tggtggggga gtacgcgctg gccgagcggg cccgggccgc
     2641 ctcggacacc ctggcggcgc aggttcgcaa gcacttcggc cccaacggct acccgcgact
     2701 gctcggcggt tcggcctcgg atgccgccgt gacgttcctc gtcgccccca tcgggccgac
     2761 ggaggcggac gtcgaggtgc tgacggccat cgaccgggcc cagacctcga tgctgcgccc
     2821 ggccgggggg ctggcgcccg gcgcctcgtg gaagaacgac ggcatcagct ggaccccgac
     2881 caccgcgctg ttcgccgccg cgtgggccgc gaacggctac ccgagcaagg ccgagatcct
     2941 cctgaaatgg ctcggtgacc accgcaccga ggccggctcc taccccgaga aggtgctgca
     3001 cgacggccga ccggccgcgg tcgcccccct cgcgtggacg gcatccctcg tggtcatcgc
     3061 cacccacgag ctggtgcggg gatgatgcgc cgcgccctcg tcgccgccat ggctctcgtg
     3121 gccgcgaccc tcggcctcgc ggccacacca tgggcgtggg cgacggcttc cgccgcgacg
     3181 tccgcgacga cagccacggc ctcaaacacc ccgccactcg tcatcgtcgg cgcgcccggg
     3241 ctcgcctggt ccgacctcga gcgcgacgac ctgccggccc tcacggcgct cgtcgagcag
     3301 ggcgcctccg ggtcgctgac cgtgcgggcg gtgcgcagcc gctcctgcgc catcgacggc
     3361 tggctcaccc tctccgccgg gcgacgcgcg ggggacctgc ccgggccgtg ccgcgagccg
     3421 ctgcccccgg tcggtgggct cgtgccgtac tggtcggact acctggctgc tgccgctgca
     3481 gattcctacg acgccagacc ggggctgctc gccgaggcgg tcgcggcatc cggggcgtgc
     3541 ctgcagagca gcggggcggg cgcggcgatc ggtgctgccg accctgacgg ctcggtgggg
     3601 tcgacgtact ccgacacggt gccgaccagt gtcgagtgcc ccgtggtgct cgtcgacggg
     3661 ggagcgctgc cggtcaccca gcccgagcgg gcggatgccg tggcgcgcct cgacgcgctc
     3721 gtcgcccagg tcgtcggggc ggcacccggg gccgacgtcg tcgtggccgg gctcggcgac
     3781 ggtgactcca gcgtgcgacc gcgggcggtc gtggcgagcg gtccgtcgtt cggcgagggc
     3841 ctgctgacct cgggctcgac gcggcagccc ggcgccatcc agctccagga cctcacggcc
     3901 acggccctcg agcgggtcgg ggtcgaggac gcaccgatca gcgggcgggc cgtgtccgtg
     3961 gtctcggggg cctcgtcggc cggtcagcgg gtcgatggcc tcgtcgggtt cgagacgcgg
     4021 gccgcgaccc tgcgatcggt gagcccgcag gtgacggcct ggctggccgt gggcttcgcc
     4081 ctctgggcgg ccctgggtgc gtggagctgg tggcgtcgcg gggcgtcgcg agagctgccc
     4141 cgggcgctgg tcgtcggcgg tgtcgcggtg gctgccgtgc ccatctcgac gtttgccgcg
     4201 aacctggtgc cctggtggca gatgccggcc ccggccgcgg tgttcgtcgc gatcctcgtg
     4261 gcggtgaccg cgctggtggc ggcggtcgcg ctgggtgccg agcgacggca tccgctgggt
     4321 gcgctgcgca ccctggcgct gttcacggtg gtggtgctcg ccggtgacgt gctcctcggg
     4381 tcgcggctgc agctcgggtc ggtcttcggg cagaacccga ccgtcggtgg gcggttctac
     4441 ggtttcggga acacgagctt cgcgctctac gggctggcgg cgctcgtgct cgtcgcctgg
     4501 gtcgcgaggg cgcggtggcg ctcgcgccag gtcgcggtgg ggctggggct cgggctgctc
     4561 gtcgtgctgt tggcgatcga gggcctgccg tcgctcggtg ccgacttcgg cggcccgccg
     4621 gggctgctgc tcggtgggct cgtcgtcatc gccggtgtcg ctggcgtccg gctgacgctg
     4681 cgccggtcgg tggtggctgt cgtggcggcc gggctgctcg tcggggcgat ctcctggctc
     4741 gactggcggc gcccgcaggc gtcgcgcacc cacctcggcg agttcgtcga cacggtgatc
     4801 tcgggggagg cgggctcggt gctcgggcgc aagctcgcgc agaacctcac caacctcggg
     4861 tccccgccgc tgctggccat ctcgatcgcg acggtggttc tcgtggtggt ggcctggcgg
     4921 gtcggctggc gcccggtgga tgccggggtg ccggtgctgc gcggggccgt cgtcctcgcc
     4981 ctggtcgggt tcgcggtcaa cgactcgggc ctggtcatcc cggccttcat cgccgtcgtg
     5041 ttggccccga cgctcgtcgc cgccggggcg gcatcggatg ggcgcggcgg ctcgcagaac
     5101 gctggctcgg ccgagcccgg tgtgccacgc tgagagcatg tcgaacacga tgccggggtc
     5161 ggggccgtgg cggccgacga gctacgtggg ccccctggtc atgctcctga tgggggtcgg
     5221 gttcctcgcc ctgggctggt gggccaacgc tcaggccgag gtcgttcggg ccaccgacgt
     5281 gtgggcctac aactcgttgg ctgcggtggc gctgctcacc ggggcgctgt tcctgccctt
     5341 tgccggcgcc accttcgtgc gggcgatgcg caaccgcaag gccctggtgc gctgacccgt
     5401 cgagcccgtc agcgcgccgt cgtgaccgtc agtgggctga gtcggccggc tcgacgagct
     5461 cgacgaggat gccgcccgcg tccctggggt ggatgaagtt gacccggctg ttgctcgtgc
     5521 cgcgccgggg cgcgtcgtag agcaggcgca acccacgctc tcgcaaggtg gcgctcacgg
     5581 catccagatc ggtgacgcgg taggcgagct gctggatgcc ggggccgctg cgatcgagga
     5641 acttcgcgat cgtcgaggcg ggggtgagtg gagccaggag ctggatgcag gacccggagt
     5701 cgccgacggc catcatcgcc tcgcgcacgc cctgctcctc gttggtctcc tcgtggagca
     5761 tccgcatccc gtaggtcgag gtgtagaagg cgatcgcctc gtcgaggtcg ggcacggcga
     5821 cgccgacgtg gtcgatcgcg gtgaacaggt cggtgggcag cgcagtcatg gcccacagcg
     5881 taggaagcgc ggatgccgcg cgcacacctc acgtggcgag catcacccgc cgcgtcggtc
     5941 gagccctgtg gcagaggtgt caaggccact tgacataccg ctgacggtga gtaaagtctg
     6001 ctttacgtca agcacgcttt acttggaggg gattgccatg cgggtcacga tgc
//
LOCUS       DAOSOC010000153         5989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_13386_length_5989_cov_11.695652, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000153 DAOSOC010000000
VERSION     DAOSOC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13386_length_5989_cov_11.695652"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1083
                     /locus_tag="PL014_10555"
     CDS             172..1083
                     /locus_tag="PL014_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOT57573.1"
                     /translation="MRWTPDQMPDLTGRRAVVTGANSGIGLVEARELARHGADVVLAV
                     RSTDAGEAAAERIRATGAAGRVSVERLDLASQESVREFAARLDGPLDLLVNNAGVMAP
                     PKHRTTVDGFELQFGTNHLGHFALTGLLLPHLLEAEAPRVTTVASIAHHRGDGAVAEG
                     NPASGYDPQTSYGRSKLANLLFALELQRRATASGSSLTSTAAHPGVSGTNLFGSRDGM
                     GSIPVIGTVGSWAVKLAFPSPERGAEAILYAATVAEPASYSGPTGIGETRGAVGTAKQ
                     SQWAQDPALAALLWERSEELTGVHYSF"
     gene            1183..2835
                     /locus_tag="PL014_10560"
     CDS             1183..2835
                     /locus_tag="PL014_10560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOT57574.1"
                     /translation="MSLRRTAISRMKNGHVDVLVVGAGINGAVTAAALAGRGASVALI
                     DRADFGGFTSQESSNLVWGGFKYLENYELPLVFGLCKSRNRLMKAYPDNVKEISFLAT
                     LDNTAPFKPWFAALGATAYWGIGQFGTKPPRLYNAEKLEAVEPVIDTTSARGAVQYQD
                     CYLVDNDSRFVFSFVRSAIEAGAAVANYVELVSARREGDRWVCQLRDTDSGEEFTTTA
                     RVVVNAAGPFVDELNTSWGLTTDHRIVYSKGIHLIVPRLTTTRHNRVLAFFDDTQRLF
                     YVIPMGRRSVIGTTDTRVDDPHTHVTDEDRDFLLAQINARLDLPEPLTHDDIIAERSG
                     VRPLVVRRGGSDQTNVDWTSLSRKHEIERDDARGVVTIFGGKLTDCLNVGEEVAEHVE
                     RLGIPLEKDLRNWYGEPAAATRKEFYRQARLMRLDSLRTKPDTEPLTDRLWRRYGRRA
                     FDLLEMIRADPTMGEDVMGSADYLRAELHTAAEHEMVVTLDDFMRRRSKIDLVVRDRD
                     ILGSPGLTEVAGILFGDDADARLADYVLSKTSHHEPPADAAG"
     gene            2931..3587
                     /locus_tag="PL014_10565"
     CDS             2931..3587
                     /locus_tag="PL014_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT57575.1"
                     /translation="MRVIVIGGHGNVARLLTPLLVAGSHEVTGVIRDPAHVEDLRADG
                     ATPVVADVESMDVEALARLVHGHDAVVWSAGAGGGDPERTYAVDRDAAIRSMEAAGRS
                     WVKRYVMVSYFGAGPDHAVPEDNAFFAYADAKSAADAHLEGSGLDWTILRPSRLTHDA
                     PTGRIETSRTGAEPGSVSRADVALVIAQALERPDLAGTVVEFNNGTVPVAEELDASAA
                     "
     gene            3691..4452
                     /locus_tag="PL014_10570"
     CDS             3691..4452
                     /locus_tag="PL014_10570"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HOT57576.1"
                     /translation="MTTTSSAHSPSGPPPQDSPRPISRAARTAFAANAAIAWLGVVLT
                     VILSGLGAYTEVTVEPGLYGGHPAGVAGALPRLADTASYFTIWSNVVVAVTMTLLARQ
                     PLRATPLLRVLRLDSILMITITAIVYAVLLAPVTEVVGWSLLTDPILHQVTPIVTVLV
                     WLVFGPRGWITGRTVLAAMVIPIAWIVWVMVRGSITSTYPYGFVNVAEYGVGPVAGTL
                     AGILAFGLLVMAIYWGVDAALRRWLGQRAGATQIS"
     gene            complement(4445..5251)
                     /locus_tag="PL014_10575"
     CDS             complement(4445..5251)
                     /locus_tag="PL014_10575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT57577.1"
                     /translation="MELTGNVFVVTGGGNGIGREVVLGLLTRGARVAAVDLSEQGLAE
                     TAALAGAGERLSTHTVDITDRAAVEVLPDAVISAHGRVDGLLNVAGIIQPFVRFADLE
                     YSHIEKVMAVNFWGVVHTTKAFLPHLRSRPQAAVVNVSSMGALAPVPGQAVYGASKAA
                     VMLLTEALYAELKGTPVTVSVVFPGGVSTHIAENSGAAIPGRSTDAAASAGSLTTAPD
                     AARQIIDKAVVAGSFRVVIGKDARMLDSMSRLSPRRATDFIAKKMAGLLS"
     gene            complement(5276..>5989)
                     /locus_tag="PL014_10580"
     CDS             complement(5276..>5989)
                     /locus_tag="PL014_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOT57578.1"
                     /translation="VAATALTVATTWSAQTSGTRLFALTRRDLGTGLLANTVALLGWD
                     AIYYWNHRFNHESRWLWAMHVVHHSSERYNLSTALRQPVAEGLTMSVPYGLLALAGVR
                     PSIIENARALNLIYQFWIHTEAVRSIGWLENVLNTPSHHRVHHGTNRQYLDRNHGSIL
                     ILWDRLFGTFEREDERVVYGLTTNIDTFNPVVIATHEWRDIGRDIAGATTWRERWSFL
                     LRGPGWAYDRRAGLVDART"
BASE COUNT          883 a         2189 c         2045 g          872 t
ORIGIN      
        1 cgagcgtgag ggtgccgccg gggccgacgg gctcgtcgag ggctcctgac ccgagggagg
       61 cgaggcgttc cccgagaccc aggcgaccgg catccgccat ccctcggaca ctcccccgca
      121 acgaccagcg catactgaat gcccgctcct gagctgaacc gaggacgccc catgcgctgg
      181 acccctgacc agatgcccga cctcaccgga cgtcgcgccg tcgtgaccgg cgcgaactcc
      241 ggcatcggcc tggtcgaggc gcgtgagctc gcccggcacg gcgccgacgt cgtgctcgcc
      301 gtgcgcagca cggatgccgg tgaggccgcc gctgagcgca ttcgggccac cggcgccgca
      361 ggccgggtca gcgtggagcg cctcgacctg gcgtcgcagg aatcggtgcg ggagttcgcc
      421 gctcgcctcg acgggccgct cgacctgctc gtcaacaacg ccggggtgat ggccccgccc
      481 aagcaccgca ccaccgtcga cgggttcgag ctgcagttcg gcacgaacca cctgggccac
      541 ttcgcgctca ccgggctgct gctgccgcac ctgctcgagg ccgaggcgcc gcgggtgacg
      601 acggtggcct cgatcgccca ccaccgcggt gacggcgcgg tcgccgaggg caacccggca
      661 tccggctatg acccgcagac gtcctacggc cgctcgaagc tggccaacct gctgttcgcc
      721 ctcgagctgc agcggcgggc gacggcatcc ggttcgtccc tgacgtcgac ggccgcgcac
      781 ccgggcgtct ccgggacgaa cctgttcggc agccgcgacg ggatgggctc gatccccgtc
      841 atcggcaccg tcggcagctg ggccgtcaag ctcgcgttcc cctcgccgga gcggggggcc
      901 gaggcgatcc tgtatgccgc gaccgttgcc gaaccggcct cgtactcggg cccgaccggc
      961 atcggcgaga cgcgtggcgc ggtcggcacg gccaagcaga gccagtgggc gcaggacccc
     1021 gccctggcgg ccctgctctg ggaacgcagc gaagagctga cgggcgtcca ctactcgttc
     1081 tgacaccaca cgccgtccgc cccgaagggg ggccagacgc gcagccagtc gcgcgtctgg
     1141 ccccctcgcc cgcgccacgc gcggatctgg gacgcttggc ccatgtcact gcgccgcacc
     1201 gccatcagca ggatgaagaa cggccacgtc gacgtcctcg tcgtcggtgc cggcatcaac
     1261 ggggcggtga ccgcagcggc tctggccggt cgcggcgcct cggtggccct gatcgaccgg
     1321 gccgacttcg gcgggttcac cagccaggag tcgagcaacc tcgtgtgggg tggcttcaag
     1381 tacctcgaga actacgagct gccgctggtg ttcgggttgt gcaagtcgcg caaccggctg
     1441 atgaaggcct acccggacaa cgtcaaggag atctccttcc tggcgacgct ggacaacacg
     1501 gcccccttca agccgtggtt cgccgccctg ggcgcaacgg cctactgggg catcggccag
     1561 ttcggcacga agccgccgcg gctctacaac gccgagaagc tcgaggccgt cgagccggtc
     1621 atcgacacca ccagcgcccg cggcgccgtg cagtaccagg actgctacct ggtcgacaac
     1681 gactcccggt tcgtcttctc cttcgtccgc tcggccatcg aggcgggggc ggccgtcgcc
     1741 aactacgtcg agctcgtgag cgcccggcgc gagggcgacc gctgggtgtg ccagttgcgc
     1801 gacaccgact cgggcgagga gttcacgacg acggcccggg tcgtcgtcaa cgcggccggc
     1861 ccgttcgtcg acgagctcaa cacctcatgg gggctcacga cggatcaccg catcgtgtac
     1921 tccaagggca tccacctcat cgtcccacgc ctcacgacca cgaggcacaa ccgggtgctc
     1981 gcgttcttcg acgacaccca gcggctcttc tacgtcatcc ccatgggccg gcgttcggtg
     2041 atcggcacga ccgacacccg cgtcgacgac ccccacaccc acgtcaccga cgaggaccgc
     2101 gacttcctcc tcgcccagat caacgcgcgc ctcgacctgc ccgagccgct gacccacgac
     2161 gacatcatcg ccgagcgctc tggcgtgcgc ccgctcgtgg ttcgtcgcgg cggcagcgac
     2221 cagaccaacg tcgactggac gtcgctgtcg cgcaagcacg agatcgagcg cgacgacgcc
     2281 cgaggcgtcg tgaccatctt cggcggcaag ctcaccgact gcctcaacgt cggtgaggag
     2341 gtcgccgagc acgtcgagag actcggcatc cccctcgaga aggacctgcg caactggtac
     2401 ggcgaaccgg ccgccgcgac ccgcaaggag ttctaccggc aggcacgcct catgcgcctg
     2461 gactcgctgc gcaccaagcc cgacaccgag ccgctcaccg atcggctctg gcgacgctac
     2521 ggccgccgcg ccttcgacct gctcgagatg atccgcgccg acccgacgat gggtgaggac
     2581 gtcatgggct ccgccgacta cctgcgcgcc gagctccaca cggccgccga gcacgagatg
     2641 gtcgtgaccc tcgacgactt catgcgccgc cggagcaaga tcgacctcgt cgtgcgcgac
     2701 cgcgacatcc tcggctcccc cgggctcacc gaggtggccg gcatcctctt cggggatgac
     2761 gcggacgcgc gactcgcgga ctacgtcctg tccaagacca gccaccacga gcccccggcc
     2821 gacgcagcgg gctgacgcac tgggctcggc gcttcgaggg cgaccgcacc acgaagccga
     2881 gggcgaccgc cggagggaac agcacggcat ccgacgaggt tggaccccca atgcgcgtca
     2941 tcgtcatcgg cggccacggc aacgtggcca gactcctcac ccccctgctc gtcgccggat
     3001 cccacgaggt caccggggtc atccgcgacc ccgctcacgt cgaggacctg cgcgcggacg
     3061 gcgccacccc tgtcgtcgct gacgtcgagt cgatggacgt cgaggccctg gcccgtctcg
     3121 tccacggaca cgatgcggtc gtctggtctg ccggtgcagg cggcggcgac cccgagcgca
     3181 cctacgcggt cgaccgggac gccgcgatca ggtcgatgga ggccgctggc cgctcgtggg
     3241 tgaagcggta cgtgatggtg tcctacttcg gggccggccc cgaccacgcg gtccccgagg
     3301 acaacgcctt cttcgcctat gccgacgcca agtcggccgc cgacgcccac ctcgaggggt
     3361 cgggcctgga ctggacgatc ctgcgtccca gccgcctcac gcacgacgca ccgaccgggc
     3421 gcatcgagac ctcacgaacg ggtgccgaac cggggtcggt gtcgcgcgcc gacgtggcgc
     3481 tcgtcatcgc gcaggccctc gagcggccgg acctggccgg taccgtcgtg gagttcaaca
     3541 acggcacggt gccggtcgcc gaggagctcg acgccagcgc tgcctgagga cggccgccca
     3601 ggtgctccca cccgtcgaca caccgttgga tgccgttcgg cctccccatc acgctccggc
     3661 ccgacctcgg ccctcgtagg ctggctcacc atgacgacga cctcctcggc ccactccccg
     3721 tccgggccgc cgccgcagga ctcgccgagg ccgatctcga gagcggcgcg cacggcattc
     3781 gccgcgaatg ccgccatcgc ctggctcggc gtcgtgctca ccgtcatcct ctccgggctc
     3841 ggtgcgtaca ccgaagtcac ggtcgagccg ggcctgtacg gcggtcatcc cgccggggtc
     3901 gccggcgcgc tgccgcgact ggcggacacg gccagctact tcacgatctg gtccaacgtc
     3961 gtcgtcgccg tgacgatgac gctgctggct cggcaaccgc ttcgggcgac gcccctgctg
     4021 cgggtcctgc gcctcgacag catcctgatg atcaccatca cggcgatcgt ctacgcggtg
     4081 ctcctggccc cggtcacgga ggtggtgggt tggtctctgc tgaccgaccc gatcctgcac
     4141 caggtgaccc cgatcgtgac ggtgctcgtg tggctcgtgt tcggaccgcg cggttggatc
     4201 accggtcgca ccgtcctggc cgcgatggtc atccccatcg cgtggatcgt gtgggtcatg
     4261 gtgcgcggca gcatcaccag cacctacccg tacgggttcg tcaacgtcgc cgagtacgga
     4321 gtgggcccgg tcgccggcac gttggccggc atcctggcgt tcggcctgct cgtcatggcg
     4381 atctactggg gtgtcgacgc cgccctgcgc cgctggctcg gccagcgtgc aggggccacg
     4441 cagatcagct gagcagcccg gccatcttct tggcgatgaa gtcggtggcc cggcgcgggg
     4501 acagccgcga catcgagtcg agcatgcgcg cgtccttgcc gatgacgacg cggaagctgc
     4561 cggcgacgac ggccttgtcg atgatctggc gagcggcgtc gggagccgtg gtgaggctgc
     4621 ccgccgaggc cgcggcgtcc gtgctccgac cggggatggc agccccggag ttctccgcga
     4681 tgtgggtgga gaccccaccg gggaagacga ccgacaccgt caccggtgtg cccttgagct
     4741 cggcgtacag cgcctcggtg aggagcatga cggccgcctt gctcgcgccg tagacggcct
     4801 ggcccgggac cggcgccagg gcacccatgc tcgagacgtt gacgacggca gcctgcgggc
     4861 gcgacctcag gtgcggcagg aacgccttgg tcgtgtgcac cacgccccag aagttcacgg
     4921 ccatgacctt ctcgatgtgg ctgtactcca ggtcggcgaa gcggacgaag ggttggatga
     4981 tgccggcgac gttgagcagg ccgtcgaccc ggccgtgggc ggagatgacg gcgtcgggca
     5041 ggacctcgac ggcggcccgg tcggtgatgt cgacggtgtg ggtgctcagc cgctcgccgg
     5101 cgcccgcgag ggcggccgtc tcggccaggc cctgctcgct gaggtcgacc gcggcgaccc
     5161 gggccccgcg ggtcagcagc cccagcacca cctcacggcc gatgccgttg ccgccgccgg
     5221 tcacgacgaa gacgttgccg gtgagttcca tggtcagacc tctcgattcg atggttcagg
     5281 tccgtgcgtc caccaggccg gcacgccggt cgtaggccca cccgggcccg cgcagcagga
     5341 aggaccaccg ctcccgccac gtcgtggccc cggcgatgtc acgcccgatg tcgcgccact
     5401 cgtgggtggc gatgaccacc gggttgaacg tgtcgatgtt cgtcgtcaac ccgtagacga
     5461 ctcgctcgtc ctcacgctcg aaggtgccga acagccggtc ccagaggatg aggatgctgc
     5521 cgtggttgcg gtcgaggtac tgccggttgg tgccgtggtg cacccggtgg tgggacggcg
     5581 tgttgagcac gttctcgagc cagccgatgg agcgcaccgc ctcggtgtgg atccagaact
     5641 ggtagatgag gttgagggcg cgggcgttct cgatgatgct ggggcgcacc ccggcgagcg
     5701 cgagcaggcc gtagggcacg ctcatcgtca acccctccgc gaccggttgc cgcagcgcgg
     5761 tcgagaggtt gtagcgctca ctgctgtggt gcacgacgtg catggcccac agccaccggc
     5821 tctcgtggtt gaaccggtgg ttccagtagt agatggcgtc ccagccgagc agggccacgg
     5881 tgttggcgag cagcccggtc ccgaggtcac gccgcgtcag cgcgaacagt ctcgtcccac
     5941 tcgtctgcgc cgaccaggtg gtggccacgg tcagggcggt cgcggccac
//
LOCUS       DAOSOC010000154         5964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_13455_length_5964_cov_8.186495, whole genome shotgun sequence.
ACCESSION   DAOSOC010000154 DAOSOC010000000
VERSION     DAOSOC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13455_length_5964_cov_8.186495"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PL014_10585"
     CDS             <1..398
                     /locus_tag="PL014_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT57579.1"
                     /translation="GPKRVILISLGALVILGIAIFVLHDGGPSTFWVLGLLMTVFVGP
                     AQAASRSFLARLIPEGKNGEIFGLYATTGRVVSFLSPAAFGVFIAIGAAVTGQENTQY
                     WGILGIVLILAAGFAVMLPVKEHGEHSVS"
     gene            complement(411..1913)
                     /locus_tag="PL014_10590"
     CDS             complement(411..1913)
                     /locus_tag="PL014_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT57580.1"
                     /translation="MLWAHRIGTAVGAAALAAALVGTVSTAAAQSPATARFPGVFATD
                     PTDPLPSDQPSPTPSVEPTPTPTPETSSTPDPAPTPGTSPSPKPTPKPSVTPIPPAAP
                     IRPPASSSEPGRQSSGGGTGGVVDYAPLTGIYLNSQIAEADRITAALSASTSKVAVAT
                     RKMDLLAEKSNALLESLAAAKESERGAIEEAARAKADLEVLDAKLEKARDVVREWVFL
                     VYSGGAGSSDVAFMIEAMASRAEDVGDPLGDLSYLTEQRTRALQDVRVLTAEQVRLTA
                     ASQAAVAEAAVATVKIQSDSSALTKVVAAQRELVGELRALQIDEVEKAGPIASALVGA
                     RSPKAKAAAERLRDALSAVSGEVTDIGEPCTDDTGLYPNGLYPPSALCPLWGAPGESL
                     SPAAAAAFNALSKSYAAQTGEPLCVTDSYRSLAGQIAIKAKRGHFAATPGTSNHGLGR
                     ALDLCGGVQDFSSPAHRWMKQNGPLFGWFHPSWAAADGRKPEPWHFEFAG"
     gene            2191..2514
                     /locus_tag="PL014_10595"
     CDS             2191..2514
                     /locus_tag="PL014_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT57581.1"
                     /translation="MGDDVNVVKLVGRVSAVGEPRELPSGDVVVMVRVVVSRPPRRAR
                     GATVDTIDVACWSARARRAASRCAVGDEVEVEGSLRRRFFRAGGGVASRYEVEASAVK
                     RRRGA"
     gene            complement(2523..3122)
                     /locus_tag="PL014_10600"
     CDS             complement(2523..3122)
                     /locus_tag="PL014_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HOT57582.1"
                     /translation="MSRTTLVVVAVIAALVGALGASWFMHTRNSPPSDFGADAGFSRD
                     MQAHHAQAVEMALLVRERSDDEELRTVAYDILTSQQHQAGQMYGWLVQWGLPQTGSGD
                     PMAWVSDDHAKAHTRPDGTMPGMATREQLDQLRAADGVAAERLFLTLMIAHHEGGVAM
                     ADAALAQARTTEVRTLAAAISSAQASEITLLQRMLDERG"
     gene            complement(3166..3801)
                     /locus_tag="PL014_10605"
     CDS             complement(3166..3801)
                     /locus_tag="PL014_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HOT57583.1"
                     /translation="MSASSKGKGGARAGRAKVDELRQAQQAKERRSRLILIGAVTTVV
                     ALIAGVVGFTVWRDVSTRPSLDAVKKFDVTREHTTEPVTYEQSPPVGGDHHPAWLNCG
                     VYPQPVPDELAVHSLEHGAVWVTYRPDLPADQVTALEDAIPDTYMVLSPREGLPSPVV
                     ASAWGTQIELTGADDPRLEVFIKEFRQGPNTPEPGAACTGGSDGELPTTTG"
     gene            complement(3902..4018)
                     /locus_tag="PL014_10610"
                     /pseudo
     CDS             complement(3902..4018)
                     /locus_tag="PL014_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
     gene            complement(4214..5476)
                     /locus_tag="PL014_10615"
     CDS             complement(4214..5476)
                     /locus_tag="PL014_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT57584.1"
                     /translation="MSNLIRYGGSFSTFRPVRARGSHLYGADGRAVLDFTSGQLSSIL
                     GHSHPDVVAAVTESVRTLDHLHSSMLSEPVLALAERLTAMAPQELDRAMFLSTGAESN
                     EAALRLAKLVTGRHEVVAFAQSWHGVTGGAAGSTYAFGRRGYGPMGVGTFAIPAPDLL
                     RGPFRSVSGYDWRAELDYAFDLVDRQSSGSLAAFIAEPILSTGGMLELPEGYFAALAE
                     HCRSRGMLLVVDEAQTGLGRTGSMFAVERDGVSPDILTLSKTLGAGLPLSAVLTTAAI
                     EDRAHDLGYLFYTTHVSDPLPAAVGLAVLDVIVRDDLVARARDAGSRLESGLRDIMAE
                     HACVGDVRGRGLLLGMEIVSDRESMAPDHERGARISARCLDLGLSMNVVQRVGAGGVF
                     RIAPPLTVTDAELDRGLEILAEAIAGTP"
     gene            complement(5527..>5964)
                     /locus_tag="PL014_10620"
     CDS             complement(5527..>5964)
                     /locus_tag="PL014_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT57585.1"
                     /translation="EVMEVVVDGRMAHFGTYNGNPLVMAAAKAVDEICTPQALAEAEL
                     VNVRALQAIDAIIDEYELPAHTVGLGVKGCVTWSTTPVRNYRDYKATDFGLAELSWLW
                     GVNRGILTPPGLDEQWLVSLVTTDEDMSTLVEDFRSLAQALRA"
BASE COUNT          860 a         2003 c         2185 g          916 t
ORIGIN      
        1 tcggccccaa gcgggtcatc ctcatctcgc tcggggccct ggtcatcctc ggcatcgcca
       61 tcttcgtcct gcacgatggc gggccgtcga ccttctgggt gctcggcctc ctgatgaccg
      121 tgttcgtcgg gccggcccag gcggcgtcgc gaagcttcct ggcacgcctg atccccgagg
      181 gcaagaacgg cgagatcttc ggcctgtacg ccacgacggg acgcgtcgtc tcgttcctgt
      241 cacccgcggc gttcggggtg ttcatcgcca tcggtgccgc ggtgaccggt caggagaaca
      301 cccagtactg ggggatcctg ggcatcgtgc tcatcctcgc cgccgggttc gcggtcatgc
      361 tgccggtcaa ggagcacggc gagcacagcg tgagctgacg gccggctggg tcagccggcg
      421 aactcgaagt gccagggctc gggcttgcga ccatcggcag cagcccacga ggggtggaac
      481 cagccgaaca gcggcccgtt ctgcttcatc cagcggtgtg ccggagaact gaagtcctgc
      541 acaccgccgc acaggtcgag tgcccgaccc agcccgtggt tgctggtgcc gggtgtcgcc
      601 gcgaagtggc cgcgcttggc cttgatggcg atctggcccg cgagtgaacg gtaggagtcc
      661 gtgacacaga gcggctcacc cgtctgcgcc gcgtagctct tgctcagggc gttgaacgcc
      721 gcagcggccg cggggctgag actctcccct ggcgcgcccc agagtgggca gagggcactc
      781 ggcgggtaaa ggccgttggg gtacaggccc gtgtcgtcgg tgcagggctc gccgatgtcg
      841 gtgacttcac cggacacggc agacagggcg tcgcgcaggc gttcggccgc agccttggcc
      901 ttcggtgacc gcgcacccac gagggcggac gcgatagggc cggctttctc cacctcgtcg
      961 atctgaaggg cccgcaactc accgacgagc tcgcgctggg ctgcgacgac cttggtgagg
     1021 gcggagctgt ccgactggat cttcacggtt gccaccgctg cctcggcgac cgcagcctgg
     1081 gaggctgccg tcagtcggac ctgctcggcg gtgaggaccc ggacgtcctg gagtgcccgg
     1141 gtgcgctgct cggtcaggta ggacaggtca ccaagggggt caccgacgtc ttccgcccgc
     1201 gaggccatcg cctcgatcat gaacgcgacg tcggaactgc ctgcgccgcc ggagtacacg
     1261 aggaagaccc actcgcggac cacgtctcgc gccttctcga gcttggcgtc cagcacctcg
     1321 aggtcggcct tggcgcgcgc cgcctcctcg atggcccctc gctcactctc cttcgccgct
     1381 gccaaggact cgagcagggc gttggacttc tcggccagca gatccatctt gcgggtggcc
     1441 accgccacct tcgacgtgct cgccgacagg gcggcggtga tgcgatcggc ctcggcgatc
     1501 tgcgagttca ggtagatgcc ggtgagcggc gcgtagtcga cgacaccccc ggtgccgccg
     1561 ccggaactct gtctcccggg ttcgctcgaa ctggccggcg gtcggatcgg ggcagccggc
     1621 gggatcgggg tgacgctcgg tttcggcgtg ggtttggggg acggggacgt ccccggagtc
     1681 ggcgcggggt cgggtgtgct gctggtctca ggggtgggtg tcggtgtcgg ctcgacgctg
     1741 ggtgtgggcg agggctggtc gctggggagc ggatctgtcg ggtcggtggc gaacacaccg
     1801 gggaaccgag ccgtcgcggg cgactgcgca gcggctgtcg ataccgtccc gacgagggcg
     1861 gcggcgagtg ccgcggcccc caccgcggtc ccgatccggt gcgcccagag cacgtgcctg
     1921 gccttcctga tgcatccgag ggcaccacgt cgacgcccct gctgtccgac ggggtcaaga
     1981 ctaggcgctc agggcccact cgaccacggc ttcggtgaac tgatttccgc aacgggcggc
     2041 gggctgttct ccacagggac gccgagcgag ggccaccatc cacaaccggg tcgtgagggc
     2101 tgcgcagcgg ctctgggagc agcagtctgg gatgcggccg ggggacacgc ctcgggatga
     2161 agaacatgcg gcggaggacg ggggcaaatg atgggcgacg acgtcaacgt ggtgaagctg
     2221 gtggggcggg tgtctgcggt gggggagccg cgcgaacttc ccagtgggga cgtcgtggtc
     2281 atggtgcggg tggtggtgtc ccggccgccc cggcgagcac gcggtgcaac cgtcgacacc
     2341 atcgacgtcg cgtgctggtc cgccagggcg cggcgcgccg cctcgcggtg cgctgtcggc
     2401 gacgaggtcg aggtcgaggg ttccctgagg cggcggttct tccgggccgg agggggtgtc
     2461 gcgagccggt acgaggtgga ggccagcgcg gtgaagaggc ggcgtggggc gtgagatgcg
     2521 gcctagccgc gctcgtcgag catgcgctgc aggagcgtga tctccgacgc ctgggcggag
     2581 gagatcgccg cggccagcgt gcgcacctcg gtggtgcggg cctgcgccag cgccgcgtcg
     2641 gccatggcga ccccaccctc gtggtgggcg atcatcaggg tcaggaagag ccgctcggcg
     2701 gccaccccgt cagcggcccg gagttgatcg agctgttcac gtgtcgccat gcccggcatg
     2761 gtcccgtccg ggcgagtgtg ggccttcgcg tggtcatcgc tcacccacgc catcggatcg
     2821 ccactgccgg tctgagggag cccccactgc acgagccagc cgtacatctg gccagcctgg
     2881 tgctgctggc tggtcaggat gtcgtaggcc acggttcgca gctcctcgtc gtcggagcgt
     2941 tcccgcacga gcagcgccat ctcgaccgcc tgcgcatggt gtgcctgcat gtccctggag
     3001 aatccggcat cggccccgaa gtccgagggc ggcgagttcc tcgtgtgcat gaaccacgac
     3061 gcgcccaggg cgccgaccaa cgcggcgatg acggcgacca cgaccaaggt ggtccggctc
     3121 acggacggtg cagtcgggcc ggccggggaa cccctcatcg gtgcgtcacc cggtggtggt
     3181 cggcagctcg ccgtcgctgc cgccggtgca ggcagctccg ggctcggggg tgttcgggcc
     3241 ctggcggaac tccttgatga acacctcgag ccgtgggtcg tcagcacccg tcagctcgat
     3301 ctgggtgccc caggcgctgg cgacgacggg ggaggggagg ccctcccggg gggagagcac
     3361 catgtaggtg tcggggatcg cgtcctcgag cgccgtgacc tggtcagcgg gaaggtcggg
     3421 acggtaggtc acccacacgg caccgtgctc gagggagtgc accgccagct cgtcggggac
     3481 cggctgcggg tagacgccac agttcagcca ggcggggtgg tgatcaccac cgacgggagg
     3541 gctctgctcg taggtcacag gttctgtcgt gtgctcacgg gtcacgtcga acttcttcac
     3601 ggcatccagg gagggccgcg tggacacgtc gcgccagaca gtgaagccga cgacgccggc
     3661 gatgagcgcc acgacggtgg tcacggcacc gatgaggatc aggcgcgagc ggcgttcctt
     3721 cgcctgctgc gcctgccgca gctcatcaac cttggcccga cctgcgcgag caccaccttt
     3781 gcccttggac gacgcactca ccctgctgct cctttgactg ccgtggcccg atcgagggca
     3841 cattgtcacc cgagaacgtg gcaggggtgg ccatcgtgcc cgcgtggggg agttgtcgcg
     3901 ctcagatgcc gaggacagcc cgagcgatca ggaagtagat gatgagcccg ctcgcgtcga
     3961 cgaacgtggt gatgaagggg ttggagaaca gcgccgggtc gaccttgatg gccctggcgg
     4021 cgcggctgga ccctgcgagg tcagtgtggc ccacgtgacg tggtgctgct cgcgttcgcg
     4081 cgtggtgcgc ggagatggtc gtcggagcat ggatcctgag cgctcccgcc ccgagtcggg
     4141 ccgacagggc ataacgccga taatcaacat tatgtcattc ccgcggatgc cggcgacggg
     4201 tggtcctccc cccttacggt gtgccggcga tcgcctcggc gaggatctcc agcccgcggt
     4261 ccagctcggc atccgtcacc gtcagcggcg gcgcgatgcg gaagacgcca ccggcaccga
     4321 cccgctggac gacgttcatc gacaacccga ggtccaggca ccgggccgag atccgagcgc
     4381 cgcgctcgtg gtcgggcgcc atgctctccc ggtcgctgac gatctccatc ccgaggagca
     4441 gccctctgcc ccgcacgtcc ccgacgcagg cgtgctcggc catgatgtcg cgcagccctg
     4501 actcgagccg cgacccggcg tcgcgagccc gggccaccaa gtcgtcccgc acgatgacat
     4561 cgagcaccgc gaggcccacg gccgcgggca gcgggtcgga cacgtgggtc gtgtagaaga
     4621 ggtacccgag gtcatgggct cggtcctcga tggccgcggt ggtcagcacg gctgacagtg
     4681 gcagcccggc accgagcgtc ttggacaggg tgaggatgtc cggggacacc ccgtcgcgct
     4741 cgacggcgaa catcgagccg gtgcgaccga gcccggtctg ggcctcgtcg acgacgagaa
     4801 gcatcccgcg ggaccgacag tgctccgcca gcgcggcgaa gtagccctcg ggcagctcga
     4861 gcatgccccc cgtgctgagg atcggctcgg cgatgaaggc ggccaggctc ccggacgact
     4921 ggcggtcgac gaggtcgaag gcgtagtcca gctccgcccg ccagtcatag ccggacaccg
     4981 agcggaacgg cccgcgcagc aggtcgggcg ccgggatcgc gaacgtcccg acgcccatcg
     5041 ggccgtaccc gcgacgaccg aaggcatagg tcgaccccgc cgctcccccg gtgacgccgt
     5101 gccagctctg cgcgaaggcc acgacctcgt ggcgcccggt gaccagcttg gccaatcgca
     5161 gggcggcctc gttggactcg gcacccgtgc tgaggaacat cgcccggtcc agctcctgcg
     5221 gcgccatcgc cgtgagccgc tcggccagcg ccagcaccgg ctcggacagc atggacgagt
     5281 gcaggtggtc cagagtccgc accgactcgg tgaccgccgc gacgacgtcg ggatggctgt
     5341 gcccgaggat cgaactgagc tggcccgagg tgaagtcgag caccgctcgc ccgtcagccc
     5401 cgtagaggtg gctgccccgc gcccggaccg gccggaacgt ggagaacgac cccccgtagc
     5461 ggatgaggtt gctcatccgg ggatcgtcct ccacgttcgc ccctcctggt ccggggtctg
     5521 ccggtgtcag gcccgcaggg cctgcgcgag cgagcggaag tcctccacga gcgtggacat
     5581 gtcctcgtcg gtggtgacga gcgagacgag ccactgctcg tcgagtccgg ggggcgtgag
     5641 gatgcctcgg ttgacgcccc acagccacga cagctcggcc agcccgaagt cggtcgcctt
     5701 gtagtcgcgg tagttgcgca ccggggtcgt cgaccacgtg acgcacccct tgaccccgag
     5761 gccgacggtg tgtgccggca gctcgtactc gtcgatgatg gcgtcgatgg cctgcagcgc
     5821 gcggacgttg accaactcgg cctcggccag cgcctgcggg gtgcagatct cgtcgacggc
     5881 cttggcggcc gccatgacga gcgggttgcc gttgtaggtg ccgaagtgcg ccatccggcc
     5941 gtcgacgacg acctccatga cctc
//
LOCUS       DAOSOC010000155         5920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_13594_length_5920_cov_8.849275, whole genome shotgun sequence.
ACCESSION   DAOSOC010000155 DAOSOC010000000
VERSION     DAOSOC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13594_length_5920_cov_8.849275"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PL014_10625"
     CDS             complement(<1..1166)
                     /locus_tag="PL014_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57586.1"
                     /translation="MNPIVAFVVVMAVWTVSEFVARKTKSLLSSLFVASLIFLVGFLS
                     GIFPEDLLASSSLLALAGVVVGFIIVHLGTVISLDDFKKQWKTFLIGFSTVIGIGIAL
                     WVAGLIFGGRISGAAEDGYAQALDFVVAGTGALSGGTISVIIVQEAALGVALTSIAVF
                     PVLIAALQGLVGFPLTSLILRKEAARLRDEYRAGNLEAPAAPDEQAASASLLPAAFQN
                     TPGTLFVVGLVVLAALGINNLTDGVLNTFVVALIFGIALRAFKVFKPSVLAGIDAFGL
                     MMLAIMILVFGPLATVEPADVKALAFPLFLAFVFGVAGIVGFSALVGKLVGYSIPMSV
                     AIGLTSLYGFPGTMILSQEAAKGAGETPEEVAAIENSILPKMIVAGFSTVTITSV"
     gene            complement(1281..2840)
                     /locus_tag="PL014_10630"
     CDS             complement(1281..2840)
                     /locus_tag="PL014_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT57587.1"
                     /translation="MSPVAMTLLILVIAVVAFMSNRVPLGVVAIGVSVSLWLTGILDL
                     NDALAGFGDPTVIFIATLFVVGEALDSTGVTAWAGQQLLARGGESRSSLLVVVCLLVA
                     VLTALISVNGAVAALIPVVVVVATRAGLPPSQMLMPLAFSAHAGSLLALTGTPVNIIV
                     SNAAEDAGARPFAFFEFALAGIPLLAGVLLIIVFFGRRLLPDRMPATLPQDLGDLTDA
                     LREHYELPDGVLVGSGRGVTEVVIAPRSPLIGQHLFTGMATPSGDLVVLAVQRAGDDL
                     KGTDDTLRAGDTLLLSGTWEKLQEHTSGSDEVLVVDDPARLRRGVPLGRGAKRAIAIL
                     IGMVVLLATGIVPAAIAGLFAACAAVLTGVVTSAQAYRSVSWTTVVLVGGMIPLSTAF
                     VTTGTADLIASRLLDVVGSASPRLALLALCVLTMVLGQLISNTATVLIMAPIASVVAL
                     DLGASPLPFLMALSISGAASFLTPVATPANTMILEPGGYRFSDYWKLGLPLLLLFLGV
                     AVFWVPVVWSF"
     gene            2946..4466
                     /locus_tag="PL014_10635"
     CDS             2946..4466
                     /locus_tag="PL014_10635"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOT57588.1"
                     /translation="MCPNPNLPVPDDFTPVPSTSATTEVSSVEAIDASAVDVLAVVVP
                     ADGDLPSELGDLDRDTLGAAGFDGKPGSTFCLPRAGAPALLLVGAPAADDLDANALRD
                     AAATASRTSAKLGARLGIRVPASGVDAAQAGQVLTEGALLARYRYTALKAEHPEKPLS
                     ALVINVTGADSGAVADGIRTGVVTSRASAIARDLANSPPAHLTAVALADVAATLGDAH
                     GFTVEAFDREQLIEMRCGGLLGVNAGSTEEPRMLVLRYTPEGEPTGHLGLVGKGIMYD
                     SGGISIKPSDPMHLLMKMDMGGAAAVLGAFTALRDLGVPATVTGWLMCTDNMPSGTAV
                     KLGDVLTARNGTTVEIKNTDAEGRLVMMDALALAVEEGVDAVVDIATLTGAVLVALGP
                     ATAGLFGNEPGIIEQVKAAGAAVDEPVWQLPLEKKYRKTLDSEVADIANLGGPYAGST
                     TAALFLAEFVGEVPWAHIDIAGTMNVDADESWRSKGATGYGARLLAELARDFTPKA"
     gene            complement(4483..5904)
                     /locus_tag="PL014_10640"
     CDS             complement(4483..5904)
                     /locus_tag="PL014_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT57589.1"
                     /translation="MPTDPRGLRGDAPLLDAWLRFQEAAPTPFTIPGHKQRHDLVGDV
                     VAGDVPLYAGLDTMKLSRGVVGQAEAQAAHLWGADLCRFSVGGSTHGNQALALAIGRP
                     GDRVVVARTLHRSMLLGLVLAGLEPVWVRPEVDPAHGLPLGVTAAALEKALTSAPDAK
                     GVFIGEPSYVGTVGDVSALAEVAHRHGIPLVIDAAWGAHLGFHPGLPPHALQAGADAL
                     VTSAHKALPAWSQGALVLARTIRIGRAQFDAAVEATATTSPSGAILASIDAARALLQR
                     DGEALLGPVIEAVATARSRLSTIPGLVVLDGPTVDPTRLVLVLPGTGADGNEVEQDLL
                     ARGMPVEMADRDTIVAMVTMADRPEAIDELAGALAESIHTRRSVARPVVVSGVWAVDP
                     VTVMSPREAFFAPRESVPVERAVGRVSAELIAPYPPGIPVLAPGEEVTDAVVAALRAA
                     RAAGSRIAYAADPSVNTLDVVAE"
BASE COUNT          996 a         2052 c         2128 g          744 t
ORIGIN      
        1 accgaggtga tggtcaccgt cgagaacccg gcgacgatca tcttcggcag gatcgagttc
       61 tcgatggctg cgacctcttc gggggtctcg cccgcgccct tggctgcttc ctgcgacagg
      121 atcatcgtgc ccgggaagcc gtagagcgag gtgagcccga tggccaccga catggggatg
      181 ctgtagccca ccagcttgcc gaccagggcg gagaacccga cgatgccggc gacaccgaag
      241 acgaacgcca ggaacagcgg gaacgccaac gccttaacgt cagccggctc gaccgtggcc
      301 agcgggccga acacgaggat catgatggcg agcatcatca gcccgaacgc gtcgatgccg
      361 gcgagcaccg agggcttgaa caccttgaac gcccgcagtg cgatcccgaa gatcagcgcg
      421 acgacgaacg tgttgaggac gccgtcggtg aggttgttga tcccgagggc cgcgagcacg
      481 acgagaccga cgacgaagag cgtgcccggg gtgttctgga acgcagccgg cagcaaggac
      541 gcggatgccg cctgctcatc gggcgccgcg ggcgcctcga ggttgccggc gcggtactca
      601 tcgcgcaggc gggccgcctc cttgcgcagg atgagcgacg tgagcgggaa cccgaccagc
      661 ccctgaagag ccgcgatgag cacggggaag acggcgatgc tcgtcagcgc cacacccagc
      721 gcggcctcct ggacgatgat caccgagatc gttccgccgc tcagggcgcc ggtgccggcc
      781 acgacgaagt cgagcgcctg ggcgtagccg tcctcggccg ccccggagat ccggccgccg
      841 aagatcaggc cagccaccca gagggcaatc ccgatgccga tgacggtgga gaacccgatg
      901 aggaaggtct tccactgctt cttgaaatca tcgaggctga tgacggtgcc gaggtggacg
      961 atgatgaagc cgacgacgac ccccgcgagc gcgagcaacg acgagctcgc gagcaggtcc
     1021 tcggggaaga tcccggacag gaaccctacg aggaagatca gcgaggccac gaacagcgag
     1081 gacagcaagg acttggtctt gcgggcgacg aactcgctga ccgtccacac cgccatgacg
     1141 accacgaagg cgacgatcgg gttcatgcca acctccagct tgagttgtga gttgtcatcc
     1201 aaccacggcg cagcaggcca cccgggctgc ttggtggaac ccgccgcggc gcgtcgtcac
     1261 agatacccat ggacgggtcg tcagaaggac cagacgacgg gcacccagaa cacggccacc
     1321 ccgaggaaga gcaggagaag cggcagcccg agcttccagt agtcactgaa gcggtaccca
     1381 cccggctcga ggatcatcgt gttggccgga gtggccaccg gggtgaggaa ggacgccgcg
     1441 ccggagatgc tcagcgccat gaggaacggc agtggcgagg caccgaggtc gagggcgacg
     1501 acggaggcga tgggcgccat gatcagcacc gtcgcggtgt tgctgatcag ctggccgagg
     1561 accatcgtca gcacgcacag cgccagcagg gccaaccgcg gcgaggccga accgacgacg
     1621 tcgagcagtc ggctggcgat gagatcagcg gtgccggtgg tgacgaacgc ggtcgacagc
     1681 gggatcatgc ctccgacgag gaccaccgtc gtccacgaga ccgagcggta ggcctgggcc
     1741 gaggtcacca ctcccgtcag gacggcggcg cacgcggcga acaggcccgc gatcgcggcg
     1801 ggaacgatcc ccgtcgcgag cagcacgacc atgccgatga ggatcgcgat ggcgcgcttg
     1861 gctcctcgac cgagcggcac gccgcggcgc aggcgcgccg ggtcgtcgac gacgagcacc
     1921 tcgtcgctgc cggaggtgtg ctcctgcaac ttctcccagg tcccgctgag caggagggtg
     1981 tcgcccgccc gcagggtgtc gtcggtgccc ttgaggtcgt cgccggctcg ctggaccgcg
     2041 agcacgacga ggtcgccact cggggtggcc atcccggtga acaggtgctg gccgatgagc
     2101 ggcgacctgg gcgcgatcac gacctcggtc actccacgcc cggagccgac gaggacaccg
     2161 tctggcagct cgtagtgctc gcgcagcgcg tcggtgaggt cccccaggtc ctgcggcagc
     2221 gtggccggca tccggtcggg cagcagtcgc cggccgaaga acacgatgat gagcagcacc
     2281 cccgcgagca acgggatgcc ggcgagggcg aactcgaaga acgcgaaggg acgcgcaccg
     2341 gcatcttccg cggcattgct gacgatgatg ttgaccgggg tgccggtgag cgcgagcagc
     2401 gagccggcat gggcggaaaa ggccagcggc atgagcatct gggagggtgg caggccggcc
     2461 ctggtcgcca ccacgacgac gacggggatg agcgccgcga cggcgccgtt gacgctgatc
     2521 agggcggtga ggaccgccac gaggagacag acgaccacga gcaggctcga gcggctctca
     2581 ccaccgcggg ccagcagttg ctgcccggcc cacgcggtga caccggtgga gtccaacgcc
     2641 tcaccgacga cgaagagcgt cgcgatgaag atgaccgtcg ggtcgccgaa gcccgcgagc
     2701 gcgtcgttga ggtcgaggat cccggtcagc cacagcgaga ccgagacccc gatggcgacg
     2761 acgccgagag ggacgcggtt cgacatgaac gccacgactg cgatgacgag aatgagcaac
     2821 gtcatcgcga ctggactcat gcgcggagcc taaccctgtg atctaccccg tgtgcaacga
     2881 cgccggatgc cgggtcaggt cgccgcgcta gggtccagac acgtcgacct cgaggagtcc
     2941 ttcgcatgtg ccccaacccg aacctgcccg ttcccgacga cttcaccccg gtgccgtcga
     3001 cgagcgcgac caccgaggtc agctcggtcg aggccatcga tgcctcggcg gtcgacgtcc
     3061 tcgccgtggt cgtgccggcc gacggtgacc tgccgagtga gctcggcgac ctcgaccgcg
     3121 acacgttggg cgctgccggc ttcgacggca agcccgggtc gacgttctgc ctcccccgcg
     3181 ctggcgcgcc agcccttctc ctcgtcggcg cccccgcagc cgacgacctc gacgccaacg
     3241 ccctgcgcga cgccgcggca accgcctcac gcaccagcgc aaagctgggc gcgcgcctcg
     3301 gcatccgagt gcccgcgagc ggtgtcgacg cggcgcaggc cggccaggtt ctcaccgagg
     3361 gtgccctgct ggcgcgctac cgctacaccg ccctgaaggc cgagcacccg gagaagccct
     3421 tgtcggccct ggtgatcaac gtgacgggag ccgacagcgg cgcggtcgcg gacggcatcc
     3481 gcaccggtgt cgtgacctcg cgcgcgagcg ccatcgcccg cgacctcgcg aacagcccgc
     3541 ctgcgcacct gacggccgtg gccctggccg atgtcgcggc gacgctcggc gacgcccacg
     3601 gcttcaccgt cgaggccttc gaccgcgagc agctcatcga gatgcgctgc ggcgggttgc
     3661 tcggtgtcaa tgccggcagc accgaggaac cccgcatgct cgtgctgcgc tacacccccg
     3721 agggcgagcc gaccggccac ctcgggctgg tgggcaaggg catcatgtac gactcgggcg
     3781 gcatcagcat caagccctcg gacccgatgc acctgctcat gaagatggac atgggcggcg
     3841 ccgcggcggt gctcggcgcg ttcaccgcgc tgcgcgacct cggcgtgccg gccacggtca
     3901 ccggctggct gatgtgcacc gacaacatgc cgtcgggcac ggccgtcaag ctcggtgacg
     3961 tgctcaccgc gcgcaacggc accaccgtcg agatcaagaa cacggatgcc gaggggcgcc
     4021 tggtgatgat ggacgcgctc gccctcgctg tcgaggaggg ggtggatgcc gtcgtcgaca
     4081 tcgcgacgct gaccggcgcg gtcctcgtcg ccctcggtcc ggcgacggcc gggctgttcg
     4141 gcaacgagcc gggcatcatc gagcaggtca aggcagccgg cgccgcggtg gacgagccgg
     4201 tctggcagct gcccctggag aagaagtacc gcaagacgct cgactcggag gtcgccgaca
     4261 tcgccaacct cggtgggccg tacgcggggt cgacgacggc ggcgctcttc ctcgccgagt
     4321 tcgtcggcga ggtgccctgg gcccacatcg acatcgccgg gacgatgaac gtcgacgccg
     4381 acgagtcgtg gcgctccaag ggcgcgacgg gttacggcgc tcgtctgctc gccgagctgg
     4441 cacgggactt caccccgaag gcctgacccc caccgtccgc ggttactcgg cgacgacgtc
     4501 gagggtgttg acgctcggat cagcggcgta ggcgatccgg ctgcccgcgg ctcgtgccgc
     4561 ccgcagggct gcgacgacag cgtcagtgac ctcctcgccg ggtgcgagca cggggatgcc
     4621 gggcgggtag ggcgcgatga gctcagcgct cacccgcccg accgcccgct ccaccgggac
     4681 gctctcgcgg ggcgcgaaga acgcctcgcg agggctcatc accgtcaccg ggtccaccgc
     4741 ccacacccct gacacgacga ccgggcgcgc cacggagcgc cgggtgtgga tcgactcggc
     4801 cagcgcgccc gcgagctcgt cgatggcctc ggggcggtcg gccatcgtca ccatcgcgac
     4861 gatcgtgtcg cgatcagcca tctcgaccgg catcccccgt gccagcaggt cctgctcgac
     4921 ctcgttgccg tcggcgccgg tgccggggag cacgagcacg agccgggtgg ggtccaccgt
     4981 cgggccgtcg aggacgacga gacccggaat cgtcgagagc cgcgagcgtg ccgtggcgac
     5041 cgcctcgatg accgggccga gcagggcctc gccgtcgcgc tggagcagtg cccgtgcggc
     5101 gtcgatgctc gcgaggatgg cccccgaggg gctcgtcgtc gccgtcgcct cgaccgccgc
     5161 gtcgaactgc gcccgcccga tgcgaatggt gcgagccagc acgagggcac cctgcgacca
     5221 cgccggcaac gccttgtgcg cactggtcac caacgcgtca gccccggcct ggagggcgtg
     5281 tggtggcagc ccggggtgga acccgaggtg ggccccccag gcggcgtcga tgacgagcgg
     5341 gatgccgtgt cggtgggcca cctccgcgag cgcgctgacg tcgccgacgg tgcccacgta
     5401 cgacggctcg ccgatgaaga cacccttggc gtccggggcg gacgtgaggg ccttctcgag
     5461 cgcggcggcc gtcaccccga gcggcaggcc gtgggccggg tcgacctctg ggcgcaccca
     5521 gacaggctcc aggccggcaa gcacgaggcc gagcagcatc gagcggtgca gggtgcgcgc
     5581 gacgacgacc cggtctcccg gacgcccgat cgcgagcgcg agcgcctggt tgccgtgggt
     5641 cgacccgccg acggagaagc ggcacaggtc ggccccccac aggtgcgcgg cctgggcttc
     5701 ggcctgcccg acgacacctc gcgagagctt catcgtgtcg aggccggcgt acagggggac
     5761 gtcaccggcg acgacgtccc cgacgaggtc gtggcgctgc ttgtgccccg ggatcgtgaa
     5821 gggggtcggg gcggcctcct ggaagcgcag ccacgcgtcg agcaggggcg cgtcgccgcg
     5881 caggcctcgg ggatcggtgg gcatgggcct cagtctgccc
//
LOCUS       DAOSOC010000156         5897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_13668_length_5897_cov_9.396611, whole genome shotgun sequence.
ACCESSION   DAOSOC010000156 DAOSOC010000000
VERSION     DAOSOC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13668_length_5897_cov_9.396611"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1295
                     /locus_tag="PL014_10645"
     CDS             63..1295
                     /locus_tag="PL014_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOT57590.1"
                     /translation="MSQTPQRTDVLVVGAGPAGSSAAAWAARAGRDVVLADAAVFPRD
                     KTCGDGLTPRAVAELDRLGLGQWVREHTVNQGLRAHGFGQTLHLRWPGGSLPDHGSAV
                     PRAELDDRIRTQALDSGATGVQGAKAVDARVEGGRVRAVTFTKGAETFEIECERLVVA
                     DGVRSGLGKVLGRQWHQDTVYAVAGRCYVDSARADDPWISSHLELRSETGETLPGYGW
                     IFPLGGGQVNVGVGTLATTKRPADVAIKPLMKHYTDLRRDDFALTGEQRMPTSALLPM
                     GGAVSNVAGANWALIGDAAACINPLNGEGIDYGLETGRLLVDHLDDDLSVVWPALLQE
                     NYGEAFSIARRLAGVFTTPKILSALGPVGMRSDWLMTLALRWMGNLITEEDRDRSARV
                     WRWAGRRSVAVDARPPFS"
     gene            complement(1320..2639)
                     /locus_tag="PL014_10650"
     CDS             complement(1320..2639)
                     /locus_tag="PL014_10650"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HOT57591.1"
                     /translation="MTSLPAGDRPSDPVASAAPLVSRTVPLPPELGTALLALLPARGA
                     RDLCSWVRRGEGVVGWGRAASHTASGPDRMAELDQWWSGLMAHAVVRDEVRRPGTGPI
                     AFGSISYSASSPAGATLVVPEVVVGVRGGAAWLTTVTVDTTPAVPGGVEPTHQPAPQG
                     PADVAFADGSLSPAEWAGAVADAVQRITAGDLDKVVLARDLEVSTTGELDVRWLLHRL
                     AERYDTTWVFAVDGLVGATPELLVRREKGLVTSRVLAGTIRRTGDDDHDLALAASLAR
                     SSKDLEEHEYAVRSVADALAPHCSSMNVPEVPFVLHLSNVMHLATDVAGVLVDDASAL
                     ALAASLHPSAAVCGTPTPVADAVIAELEHMDRGRYAGPVGWIDASGDGEFGIALRCGE
                     VSATDPSRMRLFAGCGIVAGSVPEDEVAESSAKLVPMRDALTASGPA"
     assembly_gap    2650..2749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2768..3466
                     /locus_tag="PL014_10655"
     CDS             2768..3466
                     /locus_tag="PL014_10655"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HOT57592.1"
                     /translation="MSRASLDKDPRDVAAMFDDVASKYDVTNDVLSLGQDRLWRRAVV
                     AACEAVPGQVVLDIAAGTGTSSEPFADSGVDVVPADFSLGMLRVGHQRRSDLGFTAAD
                     AMHLPFADESFDVVTMSFGLRNVADPDAALREFLRVTKPGGRLVICEFSQPTNKAFRT
                     VYSNYLMRSLPAVARKVSSNPESYVYLAESIQAWPAQRGLAQRVSGAGWGEVQWRNLS
                     GGIVALHRATKPTG"
     gene            3744..4106
                     /locus_tag="PL014_10660"
     CDS             3744..4106
                     /locus_tag="PL014_10660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOT57593.1"
                     /translation="MSNPYAPIFILLALGFGFAALSVGTSLVVGPKRYNRAKLEAYEC
                     GIQPTPRAAGGGRVPVKYFLTAMLFIIFDIESIFLYPFAVAFDSLGLFALVEMVLFIL
                     TVFVAYAYVWKRGGLEWD"
     gene            4183..4782
                     /locus_tag="PL014_10665"
     CDS             4183..4782
                     /locus_tag="PL014_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOT57594.1"
                     /translation="MGLEEKLPAGFVLTTLENVVGYMRKASIWPATFGLACCAIEMMA
                     VGTPDYDIARFGMERFSATPRQADLMIVAGRVSQKMAPVVRQVYDQMPNPKWVISMGV
                     CASSGGMFNNYAIVQGVDHIIPVDIYLPGCPPRPEMLLNAIIELHAQIQGSKLGVNRI
                     AAARAAEAAALAATPTSLMAPTHDHLAHLAAPSGKNLLA"
     gene            4779..5555
                     /locus_tag="PL014_10670"
     CDS             4779..5555
                     /locus_tag="PL014_10670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOT57595.1"
                     /translation="MSPEQNSGEVEGTLDEATQAHTANIATWQDISDEAREPVKVGER
                     HGMFGATKGHDTSGYGGLVTPTLLPGASPRPYGSWFDDVADQLGTALTTGDGPGYHEA
                     IERVVVDRGELTFFVAREHLLAVARAMRDTPQLRFEMCMGVSGVHYPNETGRELHAVY
                     PLLSITNGSRRVRLEVAAPDSDPHIPSIISVYPGNDWHERETWDMFGIIFDGHPALTR
                     ILMPDDWPGHPQRKDYPLGGIPVEYKGGVVPPPDQRRAFN"
     gene            5555..>5897
                     /locus_tag="PL014_10675"
     CDS             5555..>5897
                     /locus_tag="PL014_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HOT57596.1"
                     /translation="MTTHDHDTEVFEDQGTHESFSDAYGASVDEGLDGAPVFNVSGGD
                     WNDLVDEATALHEERIVVNMGPQHPSTHGVLRLILELDGETVTEARAGIGYLHTGIEK
                     NMEFRTWVQGVT"
BASE COUNT          864 a         2123 c         2000 g          810 t
ORIGIN      
        1 gtggtctagg gatgccgggg ggcgatttcg ggcgcgcgcc ctcaccttgc gacaatggcg
       61 gggtgtcaca gaccccacag cgcaccgatg tcctcgtcgt gggggccggg ccggccggat
      121 cgtcggcggc agcgtgggct gcgcgtgccg gccgcgacgt cgtgctggcc gacgcggccg
      181 tcttcccccg cgacaagacc tgcggcgacg ggctgacccc gcgcgccgtc gccgagctcg
      241 accgcctcgg cctggggcag tgggtgcgtg agcacaccgt caaccagggc ctgcgcgccc
      301 acggcttcgg gcagacgctg cacctgcgct ggcccggcgg ttcactgccc gaccacgggt
      361 cggccgtgcc gcgcgccgag ctcgacgacc gcatccgcac gcaggcgctg gattcgggcg
      421 ccaccggggt ccagggggcc aaggccgtcg acgcgcgggt cgagggtggg cgcgttcgcg
      481 cggtcacctt caccaagggc gcagagacct tcgagatcga gtgcgagcgc ctcgtcgtcg
      541 ccgatggcgt gcggtccggg ctgggcaagg tgctcgggcg gcagtggcac caggacaccg
      601 tgtatgccgt ggcgggtcgc tgctacgtcg actcggcccg cgccgacgac ccgtggatca
      661 gcagccacct cgagctgcga tccgagaccg gcgagaccct gcccgggtac ggctggatct
      721 tcccgctcgg cggtggccag gtgaacgtcg gcgtcggcac cctcgcgacg accaagcgcc
      781 ccgccgacgt ggcgatcaag ccgctgatga agcactacac cgacctgcgc cgggacgact
      841 tcgcgctcac cggtgagcag cggatgccca cgagcgccct gctgcccatg ggcggtgccg
      901 tgagcaatgt cgccggggcg aactgggccc tcatcggcga tgccgccgcg tgcatcaacc
      961 cgctcaacgg cgaaggcatc gactacggcc tcgagaccgg ccggttgctc gtcgaccacc
     1021 tcgacgacga cctgtcggtg gtctggccgg cgctgctcca ggagaactac ggcgaggcct
     1081 tctcgatcgc gcgccggctc gccggggtgt tcacgacgcc gaagatcctc agcgcgctcg
     1141 gcccggtcgg gatgcgcagc gactggctca tgacgctcgc gctgcgctgg atgggcaacc
     1201 tcatcaccga ggaggaccgc gaccgctccg cccgggtgtg gcgctgggcc ggtcgccggt
     1261 ccgtggcggt cgacgcacgc ccgccgttca gctgacccgc tctgggtgcc gggtgagggt
     1321 caggcgggcc cggatgccgt gagcgcgtcg cgcatcggta ccagcttggc cgacgactcc
     1381 gcgacctcgt cctcgggcac cgaaccggcg acgatgccgc acccggcgaa cagccgcatc
     1441 cgtgaaggat cggttgccga cacctccccg caccgaagcg cgatcccgaa ctccccgtcg
     1501 ccagaggcgt cgatccaccc gacgggcccg gcgtagcggc cccggtccat gtgctcgagc
     1561 tcggcgatga cggcatccgc gacgggtgtg ggcgtgccgc acacggccgc cgacgggtgc
     1621 aacgaggccg ccagtgccag ggcggatgcg tcgtcgacga gcaccccggc gacgtcggtg
     1681 gccaggtgca tgacgttgga caggtgcagc acgaacggca cctcgggcac gttcatcgac
     1741 gaacagtgcg gcgcgagggc gtcggccaca gagcgcaccg cgtactcgtg ctcctcgagg
     1801 tccttgctcg agcgggccag ggatgccgcg agcgccaggt cgtggtcgtc gtcgccggtg
     1861 cggcggatcg ttcccgcgag cacacgggag gtgaccagcc ccttctcgcg gcggacgagc
     1921 agctcaggtg tggccccgac gaggccgtcg accgcgaaca cccacgtggt gtcgtagcgc
     1981 tctgcgaggc ggtgcagcag ccaccgaacg tcgagctcgc cggtggtgga cacctcgagg
     2041 tcgcgggcga gcaccacctt gtcgaggtcg ccggcggtga tgcgctgcac ggcatccgcg
     2101 acggcaccgg cccactcggc gggggagagg gagccgtcgg cgaaggcgac gtcggccggg
     2161 ccctgggggg ccggttggtg ggtcggctcg acaccgcccg ggaccgcggg ggtggtgtcg
     2221 accgtgaccg tcgtcagcca ggcggccccg ccgcggacac ccacgacgac ctcagggacg
     2281 acgagcgtgg caccggcggg cgaggaggcg gaatacgaga tggagccgaa cgcgatcggc
     2341 ccggtacccg ggcggcgcac ctcgtcgcgc acgacggcat gcgccatgag gccggaccac
     2401 cactggtcga gctcggccat ccggtcgggg ccggatgccg tgtggctggc cgcgcggccc
     2461 cacccgacga ccccttcgcc gcgtcgaacc caggagcaca ggtcgcgggc gccgcgggcg
     2521 ggcagcagtg cgagcagcgc cgtgccgagc tcggggggca ggggcacggt gcgcgagacg
     2581 aggggcgcgg cggaggcgac ggggtcgctc ggtcggtcac ccgcgggcag cgaggtcatc
     2641 gcgcagcagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtgggaggat
     2761 ggcccccatg agccgcgcct ccctcgacaa ggacccccgc gacgtcgccg cgatgttcga
     2821 tgacgtcgcg agcaagtacg acgtgaccaa cgacgtgctc tcgctgggcc aggaccgcct
     2881 ctggcgccgc gcggtcgtcg cggcctgtga ggccgtgccc gggcaggtcg tgctcgacat
     2941 cgccgcgggc acgggcacga gcagcgagcc gttcgccgac tccggtgtcg acgtcgtgcc
     3001 cgccgacttc tcgctgggga tgctgcgcgt gggccaccag cgccgttcgg acctcggctt
     3061 caccgcggcc gacgcgatgc acctgccctt cgccgacgag tccttcgacg tcgtgacgat
     3121 gagcttcggt ctgcgcaacg tcgccgatcc ggatgccgcg ctgcgcgagt tcctgcgcgt
     3181 caccaagccg ggtggtcgcc tcgtcatctg cgagttcagc cagccgacga acaaggcatt
     3241 ccgcacggtg tactcgaact acctcatgcg ctcgctcccg gcggtggcgc gcaaggtcag
     3301 cagcaacccc gagtcctacg tctacctcgc agagtccatc caggcctggc cggcgcagcg
     3361 cggcctggcc cagcgggtgt ccggggccgg ctggggcgag gtccagtggc gcaacctttc
     3421 tggcggcatc gtcgccctcc accgggccac caagccgacc ggctgaccgc ggccgcacct
     3481 cgacgtcaag agttaggagc gcctaagtaa aaggtcgccg cgcggccgtc ctagactgcc
     3541 gccgatcgtg aagagattca caagcaccct cacggcgttc gcgcgccggt ccggaaacgc
     3601 gttcacggcg tgcgtccgga ccgactgtcg agcgtcgccg ggtgaggcgt aggtcactcc
     3661 ccctaggatg gccgggacgc tgtctgttgt tcggccgtgc cagcccgatg tacctgagca
     3721 ccgaaagggg agttgtcacc tcgatgagca acccctacgc accgattttc atcctgctcg
     3781 cgctgggctt cggcttcgcc gcgctctccg tgggcaccag cctcgtcgtc ggccccaagc
     3841 gctacaaccg cgccaagctc gaggcctacg agtgcggcat ccagcccacg ccgcgcgccg
     3901 ccggtggtgg ccgcgtcccg gtcaagtact tcctcacggc gatgctcttc atcatcttcg
     3961 acatcgagtc gatcttcctg taccccttcg ccgtcgcctt cgactccctc ggcctgttcg
     4021 cgctcgtcga gatggtgctg ttcatcctca ccgtcttcgt cgcctacgcc tacgtgtgga
     4081 agcgcggcgg gttggagtgg gactgatgac caccgcccgg atgccgtccg cacccctgtt
     4141 cgtgcacccc acctcaccga ccgctcaccc caaggagggc tgatgggact cgaggagaag
     4201 ctgcccgccg gcttcgtgct gaccacgctc gagaacgtcg tcggctacat gcgcaaggcc
     4261 tcgatctggc cggcgacgtt cggtctggcc tgctgcgcca tcgagatgat ggccgtcggc
     4321 acccctgact acgacatcgc ccggttcggc atggagcggt tctccgccac cccccgccag
     4381 gccgacctga tgatcgtcgc cggacgggtc tcgcagaaga tggcgcccgt cgtgcggcag
     4441 gtctacgacc agatgcccaa ccccaagtgg gtcatctcca tgggtgtgtg cgccagctcg
     4501 ggcgggatgt tcaacaacta cgcgatcgtc cagggtgtcg accacatcat cccggtcgac
     4561 atctacctgc cggggtgtcc gccccgcccc gagatgctcc tgaacgcgat catcgagctg
     4621 cacgcccaga tccagggcag caagctcggc gtcaaccgca tcgccgcggc ccgggcagcc
     4681 gaggccgcag ccctcgctgc aaccccgacc tcgctgatgg ccccgaccca cgaccacctg
     4741 gcccacctcg cggctccgag cgggaagaac ctcctcgcat gagccccgag cagaactccg
     4801 gagaggtcga gggcaccctc gacgaggcca cgcaggccca caccgcgaac atcgccacct
     4861 ggcaggacat ctccgacgag gctcgcgagc ccgtcaaggt cggcgagcgc cacggcatgt
     4921 tcggcgcgac caagggtcac gacacctccg gctacggcgg cctcgtgacc ccgaccctgc
     4981 tgcccggcgc gagcccgcgg ccctacggct cgtggttcga cgacgtggcc gaccagctcg
     5041 gtaccgcgct cacgaccggc gacggccccg gctaccacga ggccatcgag cgggtcgtcg
     5101 tcgaccgcgg cgagctgacc ttcttcgtcg cccgcgagca cctgctcgcc gtcgcccgcg
     5161 cgatgcgcga caccccgcag ctgcgtttcg agatgtgcat gggcgtctcc ggggtgcact
     5221 acccgaacga gacgggccgc gaactgcacg cggtctaccc gctgctctcg atcaccaacg
     5281 gcagccgccg cgtccgcctc gaggtcgccg ctcccgacag cgacccgcac atcccgtcga
     5341 tcatctcggt ctacccgggc aacgactggc acgagcgcga gacctgggac atgttcggca
     5401 tcatcttcga cgggcacccg gccctgaccc gcatcctcat gccggacgac tggccgggcc
     5461 acccccagcg caaggactac ccgctcggcg gcatccccgt cgagtacaag ggcggcgtcg
     5521 tgccgccgcc ggaccagcgg agggcgttca actgatgacg acccacgacc acgacaccga
     5581 ggtcttcgag gaccagggca cccacgagtc gttcagcgac gcctacgggg cctccgtcga
     5641 cgaagggctc gacggcgcac cggtcttcaa cgtctccggc ggtgactgga acgacctcgt
     5701 cgacgaggcg accgcgctcc acgaggagcg gatcgtcgtc aacatgggcc cgcagcaccc
     5761 gtcgacgcac ggcgtgctcc gcctcatcct cgagctcgac ggcgagacgg tcaccgaggc
     5821 ccgcgccggc atcggctacc tgcacaccgg catcgagaag aacatggagt tccgcacctg
     5881 ggtgcagggc gtcacgt
//
LOCUS       DAOSOC010000157         5864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_13762_length_5864_cov_9.592012, whole genome shotgun sequence.
ACCESSION   DAOSOC010000157 DAOSOC010000000
VERSION     DAOSOC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13762_length_5864_cov_9.592012"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PL014_10680"
     CDS             complement(<1..266)
                     /locus_tag="PL014_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOT57597.1"
                     /translation="MAGTPGSERTIGQLVADATHDFQGLVRSEIALAKAEVTSGAKDV
                     GKGAGLLAAAAFFGLMGLVFLLHGAAWGLAEALPIWAAYLIVAV"
     gene            complement(391..1722)
                     /gene="nhaA"
                     /locus_tag="PL014_10685"
     CDS             complement(391..1722)
                     /gene="nhaA"
                     /locus_tag="PL014_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOT57598.1"
                     /translation="MTNSTPPRKSGTILRRPAWAESQYVAEALRTETIGGALLLAAAV
                     AALVFANSPWREAYETLRDTYVGPEWGDLRLSLGHWAADGLLAIFFFVAGLELKREFL
                     VGELRTVAKAAVPVTAAIGGVIMPALVFVGIVLALGGDSQALRGWAIPTATDIAFALA
                     VLAVIGSYLPSALRSFLLTLAVVDDLIAITIIAIFYTADLEVTALLWAVLPLAAFAML
                     VQRRITYWWLLIPLAFATWTLVHASGVHATVAGVLLGLVVPVKARASVPKISTLERDT
                     DVAHRFEHRIRPLSAGFAVPVFAFFASGVTVIGGGFAAAAADEVAIAIVCALVLGKLF
                     GVFGTTWLLARFTRAELDEGLGWRDVFGLALLTGVGFTVSLLVGELAFGEGSERNDHV
                     KLAVLTGSLIAAILASIVLRMRNTHYKQIAELENHDGDADGIPDVYQRDNT"
     gene            complement(1799..2353)
                     /locus_tag="PL014_10690"
     CDS             complement(1799..2353)
                     /locus_tag="PL014_10690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT57599.1"
                     /translation="MTRIGIVTSSTRPTRIGHHVAQWVATQAPEGVEVEPIDLRDLAL
                     PLLDEPEMPSSGKYAHEHTKRWAALVDGVDAVVFVMPEYNRGYNAALKNAIDYLYAQW
                     QGKPVACVGYGWGGAEYARAALRQTLDRVGMVVVDGAALVFEKTLSTQGAVTADDSLV
                     ADVAALYGGLVAAVPTGQDTDAAG"
     gene            complement(2465..4429)
                     /gene="acs"
                     /locus_tag="PL014_10695"
     CDS             complement(2465..4429)
                     /gene="acs"
                     /locus_tag="PL014_10695"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOT57600.1"
                     /translation="MTDDSLSSHAHELDLAPAPAFAAQANGTAEMYEAAAADHEGFWA
                     QQARERITWATDFERTLDWDDAPFAKWFVGGQLNVAYNCVDRHVEAGNGDRVAIHFEG
                     EGGDTRTITYADLQREVSKTANALTELGVGTGDTVAVYMPMIPETVFTMLACARLGAP
                     HSVIFGGFSGEALHTRIDDAQCKVVVTTDGQFRRGKPSPLKPAVDEALQYPNPVQKVL
                     VVRRTETEVDWHEGRDVWWHDVVDRQADTHEAQPHDSEHPLFILYTSGTTGKPKGIFH
                     TSGGYLTQAAYTNAVVHDVHPETDVYWCTADVGWVTGHSYIVYGPLANGATQVMYEGT
                     PDTPHQGRWWELIEKYKVSILYTAPTAIRTFMKWGDDIPAKFDLSSVRVLGSVGEPIN
                     PEAWLWYRKHIGGNTAPIVDTWWQTETGGIMISPLPGVTTLEPGSAQRPIPGISAEIL
                     DDEGMPFTEAEKVGYLVLTKPWPAMLRGIWGDPERYKDTYWTRFGPKYYFAGDGAKYD
                     EKGNIWLLGRVDDVMNVSGHRLSTAEIESALVSHPAVAEAAVVGASDETTGQAVCAFV
                     ILRGDAADEGDATVQALRNHVAHEIGPIAKPRSIMIVPELPKTRSGKIMRRLLKDVAE
                     GRDIGDATTLADSSVMSLIAKGMPGGGSSS"
     gene            4484..5689
                     /locus_tag="PL014_10700"
     CDS             4484..5689
                     /locus_tag="PL014_10700"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HOT57601.1"
                     /translation="MSNRPTAGCRRAGRGCAERGAPDRPSRPGQRDFLHSSHPAVKWL
                     ECKKCGFGGQVDQCFRMPGGAMVRALTTASGNGSIRTASVEPWRERPDRPSTMRKEAM
                     TVFEEGQLYSAVTTDADGQVQVHLGEDHPGFTDPVYRARRADIASASLAHRPGQPVPT
                     IDYTDEEHGVWALVSAELAAKHERYAHPEYLEAKAALALPTDHVPQLHEVTQALMPLT
                     GWCYTAAPGLVPLREFYADLGRRTFNSTQYLRHPSEPLYTPEPDIIHEVIGHANQLAS
                     PRFAAIAQAAGEASERLETEDALQFVADVFWFTIEFGVMNDGGVTKAYGAGILSSYGE
                     LDEFSHMDIRPLDIPTMGTLNYDITAYQPVLFEAQSMDQLEGVVGTFFETVDDDLAAR
                     MRAEAHAHA"
BASE COUNT          883 a         1991 c         2100 g          890 t
ORIGIN      
        1 atcaccgcga cgatgaggta ggccgcccag atcggcaggg cctcggccag gccccacgcc
       61 gcgccgtgga gcaggaacac caggcccatg aggccgaaga acgctgccgc ggcgagcagg
      121 ccggcaccct tgccgacatc cttggcgcca ctggtcacct cggccttggc cagcgcgatc
      181 tcgctgcgga ccaagccctg gaagtcatgc gtcgcatcgg ccacgagttg accgatcgtg
      241 cgctcgctgc ccggtgtgcc tgccatgaat cctccgccgt ggtcccgctg tgtcgaaagc
      301 cgtctccccg accctactcg ggaccccgcc gtcgggtgct gggacggtcg cgccggcacc
      361 cgtgagcacc ctattcaggg agcggttggg tcaggtgttg tcgcgctggt agacgtcggg
      421 gatgccgtcg gcgtcgccgt cgtggttctc gagctcggcg atctgcttgt agtgcgtgtt
      481 gcgcatccgc aacacgatcg aggcgaggat cgcggcgatc agggaccccg tgagcacggc
      541 cagcttgacg tggtcgttgc gctcggagcc ctctccgaag gccagttcgc cgacgagcag
      601 ggagacggtg aagccgaccc cggtgagcag ggcgaggccg aagacgtcgc gccaacccag
      661 cccttcgtcg agctcggccc gggtgaagcg ggccagcagc cacgtcgtgc cgaagacccc
      721 gaagagcttg ccgagcacga gggcacacac gatggcgatg gccacctcgt cggcggctgc
      781 cgcggcgaac ccgcccccga tcacggtcac tccggaggcg aagaaggcga acacgggcac
      841 ggcgaagccg gccgagagcg ggcggatgcg gtgctcgaac cggtgggcga cgtcggtgtc
      901 gcgctcgagg gtgctgatct tgggcacgga ggcccgtgcc ttgaccggca cgaccagccc
      961 gagcaggaca ccggcgacgg tggcgtgcac ccccgaggcg tggaccagcg tccacgtggc
     1021 gaaggccaac ggaatgagca gccaccagta ggtgatccgc cgctgcacga gcatcgcgaa
     1081 tgcagccagc ggcagcacag cccagagcag ggccgtgacc tcgaggtcgg cggtgtagaa
     1141 gatcgcgatg atcgtgatgg cgatgaggtc gtcgacgacc gcgagggtga gcaggaacga
     1201 gcgcagggct gagggcaggt aggacccgat gacggcgagg acggccaggg cgaaggcgat
     1261 gtcggtcgcg gtcgggatgg cccagccacg cagggcctgg ctgtcaccgc cgagtgccag
     1321 cacgatgccg acgaagacca gggccggcat gatgacgccc ccgatggccg ccgtgaccgg
     1381 caccgcggcc ttggccaccg tgcgcagctc accgacgagg aactcgcgct tgagctcgag
     1441 cccggcgacg aagaagaaga tggcgaggag gccgtctgcc gcccagtggc ccaggctcag
     1501 ccgcaggtcg ccccactcgg ggccgacgta ggtgtcacgc agcgtctcgt aggcctcacg
     1561 ccacggactg ttggcgaaga cgagagccgc gacggcagcg gcgagcagca gggccccacc
     1621 gatggtctcg gtccgcagcg cctcggcgac gtactggctt tccgcccagg ccggtcgacg
     1681 aaggatcgtg ccgctcttgc ggggtggagt cgagttggtc attgccgtcc tcgtggtcgg
     1741 tcgtcctcgg ggtcgcttca acggtgccga ccagacttcc cggcgctccg cgggcagtct
     1801 atccagccgc gtcggtgtcc tgcccggtgg gcaccgcggc cacgaggccg ccgtagaggg
     1861 ccgcgacgtc cgccaccagg ctgtcgtcgg cagtcacggc accctgtgtc gagagcgtct
     1921 tctcgaacac cagggcggcc ccgtcgacga cgaccatccc cacccgatcc aacgtctggc
     1981 gcaacgctgc ccgggcgtac tcggccccgc cccagccgta gccgacacac gcgaccggct
     2041 tgccctgcca ctgcgcgtac aggtagtcga tcgcgttctt cagcgctgcg ttgtagcccc
     2101 ggttgtactc gggcatgacg aacacgacgg cgtcgacacc gtcgacgagg gcagcccagc
     2161 gcttggtgtg ctcgtgcgcg tacttgccgc tcgacggcat ctcgggctcg tcgagcaggg
     2221 gcagggcgag atcacgcagg tcgatgggtt cgacctcgac gccctctggc gcctgggtgg
     2281 ccacccactg tgcgacgtgg tggccgatcc gggtcgggcg ggtgctggag gtgacgatgc
     2341 cgatgcgggt catcccggca tcctcacacc tcgcgagctg tcaagggccc atgcgcaacg
     2401 tccggcaggt tggtgcctga ggcaaccaac ctgccggacg tcagcgggtg cggggggcgc
     2461 gaggtcagct cgacgagccg ccgcccggca tccccttggc gatgaggctc atcaccgacg
     2521 agtcggccag cgtcgtcgcg tcgccgatgt cgcgaccctc ggcgacgtcc ttgagcaggc
     2581 ggcgcatgat cttgccggag cgggtcttgg gcagctcggg cacgatcatg atcgagcgcg
     2641 gcttggcgat cgggccgatc tcgtgggcca cgtggttgcg cagcgcctgc accgtggcgt
     2701 cgccctcgtc ggcggcgtcg ccgcgcagga tgacgaaggc gcacacggcc tgaccagtgg
     2761 tctcgtccga cgccccgacg accgcggcct cggccaccgc cgggtgcgac accagcgccg
     2821 actcgatctc ggccgtcgac aggcggtggc cggagacgtt catgacgtcg tcgacccggc
     2881 cgagcagcca gatgttgccc ttctcgtcgt acttggcgcc gtcgccggcg aagtagtact
     2941 tcgggccgaa gcgggtccag tacgtgtcct tgtaccgctc cgggtcgccc cagatgccgc
     3001 gcagcatcgc cggccacggc ttggtcagca cgaggtaccc gaccttctcg gcctcggtga
     3061 acggcatccc ctcgtcgtcg aggatctcgg ccgagatgcc ggggatcggg cgctgcgccg
     3121 aaccgggctc gagcgtcgtg acaccgggca gcgggctgat catgatgccg ccggtctcgg
     3181 tctgccacca cgtgtcgacg atcggggcgg tgttgccgcc gatgtgcttg cggtaccaga
     3241 gccaggcctc ggggttgatc ggctcgccga cgctgccgag cacgcgcacc gaggacaggt
     3301 cgaacttcgc ggggatgtcg tcgccccact tcatgaacgt gcggatcgcc gtgggggcgg
     3361 tgtagaggat cgagaccttg tacttctcga tgagctccca ccagcggccc tggtgcgggg
     3421 tgtccggggt gccctcgtac atgacctggg tggcgccgtt ggcgagcggc ccgtagacga
     3481 tgtaggagtg cccggtgacc cagccgacgt cggccgtgca ccagtagacg tcggtctcgg
     3541 ggtggacgtc gtggacgacg gcgttggtgt aggcggcctg ggtcaggtag ccgccgctgg
     3601 tgtggaagat gcccttgggc tttccggtcg tgccactcgt gtagaggatg aacagcgggt
     3661 gctcgctgtc gtgcggctgg gcctcgtggg tgtcggcctg gcggtcgacg acgtcgtgcc
     3721 accagacgtc gcggccctcg tgccagtcga cctcggtctc ggtgcggcgc acgacgagga
     3781 ccttctgcac cgggttgggg tactgaagcg cctcgtcgac ggcgggcttg aggggggagg
     3841 gcttaccgcg ccggaactgc ccgtcggtgg tcacgacgac cttgcactgg gcgtcgtcga
     3901 tgcgggtgtg cagcgcctcg cccgagaacc cgccgaagat gaccgagtgc ggggcgccga
     3961 gtcgggcgca ggccagcatg gtgaagacgg tctcggggat catcggcatg tagaccgcca
     4021 ccgtgtcacc ggtcccgacg ccgagctcgg tcagggcgtt ggcggtcttg ctgacctcgc
     4081 gctggaggtc ggcgtaggtg atggtgcggg tgtcgccgcc ctcgccctcg aagtggatcg
     4141 cgacccggtc accgttgccg gcctcgacgt gccggtcgac gcagttgtac gcgacgttga
     4201 gctggccgcc gacgaaccac ttcgcgaagg gtgcgtcgtc ccagtccagg gtgcgctcga
     4261 agtcggtcgc ccaggtgatg cgctcgcggg cctgctgggc ccagaagccc tcgtgatcgg
     4321 cggcggcggc ctcgtacatc tcggcggtgc cgttggcctg cgcggcgaac gccggggcag
     4381 gggcaaggtc gagctcgtgg gcgtggctgg acaggctgtc gtcggtcacg gcggtgatcc
     4441 tcctcgttga gtgactggcc ctagccaacc ccagcggtgg gccgtgagca accggcccac
     4501 cgcaggatgc cgtcgggcgg gccgcggctg cgccgagcgg ggcgcccccg accgcccgtc
     4561 gcgccccggg cagcgcgact tcttgcactc cagccaccct gccgtgaagt ggctggagtg
     4621 caagaagtgc gggtttggtg ggcaggttga ccagtgtttt cggatgccgg gtggcgcgat
     4681 ggtgcgcgca ttgaccaccg cgtcgggcaa cggttccatc cgcacggcat ccgtggaacc
     4741 gtggcgagaa cggcccgatc ggccgtccac gatgcgcaag gaggcgatga ccgtgttcga
     4801 agagggccag ctctactcag ccgtcacgac cgacgccgac ggccaggtgc aggtgcacct
     4861 gggcgaggac caccccggct tcaccgaccc ggtgtaccgc gccaggcgtg ccgacatcgc
     4921 ctcggcctcg ctggcgcacc ggcccgggca gcccgtgccg acgatcgact acaccgacga
     4981 ggagcacggc gtctgggcgc tggtgtccgc cgagctcgcg gcgaagcacg agcggtacgc
     5041 ccaccccgag tacctcgagg ccaaggccgc cctcgccctg ccgaccgacc acgtgcccca
     5101 gctgcacgag gtgacccagg cgctcatgcc gctcaccggc tggtgctaca ccgcggcacc
     5161 tggactggtt cccctgcgcg agttctacgc cgacctcggt cgccgcacct tcaactccac
     5221 tcagtacctg cgccacccga gtgagccgct gtacacgccg gagcccgaca tcatccacga
     5281 ggtcatcgga cacgcgaacc agctggcgag cccgcgcttc gcggcgatcg cgcaggccgc
     5341 gggtgaggcc agtgagcgcc tggagaccga ggacgccctg cagttcgtcg ccgacgtctt
     5401 ctggttcacc atcgagttcg gggtgatgaa cgacggcggg gtcaccaagg cctacggagc
     5461 cggcatcctc tccagctacg gcgagctcga cgagttctcg cacatggaca tccgccccct
     5521 cgacatcccg accatgggca ccctcaacta cgacatcacg gcctaccagc ccgtgctgtt
     5581 cgaggcgcag agcatggacc agctcgaggg tgtcgtcggc acgttcttcg agaccgtgga
     5641 cgacgacctg gccgcccgga tgcgagccga ggcgcacgcc cacgcctgac gccctgccgg
     5701 agcgtggcgc aacagttgag cggatgccgt ggcacggcat ccgctcaact gttgcgttca
     5761 ggcgtcgatc agtggttgac cgcctcggcg acaccgtgac cggtgaggct gcggacctcc
     5821 atctcggcgt acttctgggc gtcgtgctcc ttggagagca ccga
//
LOCUS       DAOSOC010000158         5764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_14085_length_5764_cov_9.791732, whole genome shotgun sequence.
ACCESSION   DAOSOC010000158 DAOSOC010000000
VERSION     DAOSOC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14085_length_5764_cov_9.791732"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..>3029
                     /locus_tag="PL014_10705"
     CDS             106..>3029
                     /locus_tag="PL014_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOT57602.1"
                     /translation="MTDPLLRAHLAPEPRTLVDILRATADAHPDSPAIDNGREVLTYD
                     ELLDAAQELAAALRSDGVRRGDRVGVRIPSGTTDLYVAITGILLAGAAYVPVDHDDPD
                     ERARVVFGEAGVAAIVGTDLVIRSGPTARPDADPDEAPEEDPELTDDAWVIFTSGSTG
                     LPKGVAVSHRNAAAFVDAESRMFLQQRPIGPGDRVMAGLSVAFDASCEEMWLAWRYGA
                     CLVPAPRSLVRSGMDLGPWLVANDITIVSTVPTLVALWPADALADVRLLILGGEACPP
                     EIGARLATDTREVWNTYGPTEATVVACGAQLDGQPPVRIGLPLDGWDLAVVDAQGQRV
                     PDGEPGELIIGGVGLARYLDPEKDAAVYAPMPGLGWERAYRSGDVVRFDGVGLVFQGR
                     ADDQVKVGGRRIELGEVDSALLGLPGVSGAAAAVKRTEAGNRLLVGYVTADAGFDPKA
                     AAEQLRASMPAALVPRIAVVDTLPTRTSGKIDRDALPWPPPGASRGTGATASGTPDLE
                     LDGTAAWIAGHWAAILGSPPSAPDEDFFDLGGGSLSAAQLVSKLRERHPDVTVADIYE
                     HPVLADLAQTLDAMAAPTGRTNASVSPVPRDTQVAQVLGTVVVRSIGALRWLTWIGLG
                     LLVAHRVVDAPWLPRIAWGWVLTGWLLLINPFGRVLLGAAAARLVLRGVGPGRYPRGG
                     RVHLRLWLAERLVDELGATNLSAAPLVRVYAKLLGCRVGRHVDLHSIPPVTGLLTLGT
                     GCSIEPEVDLAGHWLDGGHVYIGAMAVGEEARVGARSTLLPGADIGDRAEVAPGSAVF
                     GPVPADEAWSGAPAGPSGAARGPWTPERPPNRRVWVLAYAASAIGLALVPVLAVLAGV
                     TVALPELAAATDLAGLVTAALWWVPVATAVGLVVLVGLVLGTVRLLSIGLEPGHHPVH
                     GLRAWQAWSVLRVLDEARTWLFPLYSSSLTPWWLRALGADIGPDVEASTVLMLPKFVT
                     VRA"
     assembly_gap    3030..3129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3130..3842
                     /locus_tag="PL014_10710"
     CDS             <3130..3842
                     /locus_tag="PL014_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation protein"
                     /protein_id="HOT57603.1"
                     /translation="GDTTRTHAPPTRLRVARALVELARIVAVWVHVALIVLSAAALLA
                     LVDAGALWAIALGGPVLLVAGAVAVGVTTVAKWLLVGRLRPSEHPLWSSFVWRNELAD
                     TFVEVVAAPWFARAAVGTSVLNVWLRSMGARVGRGVWCETYWLPEADLIDLRDGATVG
                     RGCVVQTHLFHDRILAMDRVTIADGGTLGPNSVILPAARIGRHATVGPVSLVMRGESV
                     PDKTRWIGNPVGPWAEPE"
     gene            3839..5146
                     /locus_tag="PL014_10715"
     CDS             3839..5146
                     /locus_tag="PL014_10715"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOT57604.1"
                     /translation="MTGSDPYLPGHGDDRYQVEHYALDLRYKPLGNHLEGRAELSVRA
                     VVDLTDLVVDLHRLEVRSLRVEGAKVARWSHRGSRLRIRLTQELPAGEPLLLTIAYRG
                     NPGTMPGPDGPAGWEELTDGVLVAAQPHGAPTWFPCNDRMADKARYRIRVETDPAYRV
                     VANGILVSHKTVGRRAEWVHEQREPMAPYLATLQIGRYDAVQQTGAPVPTWLVGPPAR
                     MQSVREALADQPRMLEVFTELFGPYPFERYTAVVTDDPLEIPLESQSLSTFGTNHLSR
                     DWSAQRLIAHELSHQWFGNAVTAASLADIWLHEGFACYAEWLWSQASGRTTLRQEAAR
                     HHDGLARKPQDLVLTDPGADRVFDDRVYKRGALTLFALHTALGDEMFFDVLRTWVDTH
                     RYEVVMTADFEACVESVSGSPHRELFDAWLRREELPELPPQRG"
     gene            complement(5064..>5764)
                     /locus_tag="PL014_10720"
     CDS             complement(5064..>5764)
                     /locus_tag="PL014_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOT57605.1"
                     /translation="IGWGVTTVAADPATLHPAEAALVDCAQPQRRAEFATGRECARTA
                     LREIGAEAPDHPILADANGAPLWPAGVIGSISHTAGWTGAVAARQGTGVRGHRTGLAS
                     IGLDAEVAVPLPEGVLDVVATPAERAALEHLGRSRADLPWDTLLFAAKEATYKAWYPL
                     TGVVLHHEQVRVHLAADGTFHAEASDGIRMPLRLQGRWRAGPDVVVTLAAVAAREVPR
                     DAATRRTARGAASR"
BASE COUNT          681 a         2187 c         1998 g          798 t
ORIGIN      
        1 cggcgcggcg aacgcagtcg tcacgcggcg ttcacctcgt cacgccagac tgggcacgtg
       61 gagccttcga cgccgtcagg cgaaccctcg cagcgtgacc tcgacgtgac cgacccgctg
      121 ctgcgtgccc acctcgctcc ggagccgcgc accctcgtcg acatcctgcg cgccaccgcc
      181 gatgcacatc ccgactcccc ggcgatcgac aacggccgtg aggtcctgac ctacgacgaa
      241 ctgctcgacg ccgcccagga gctagcggcg gcgctgcgca gcgacggtgt ccggcgtggc
      301 gaccgggtcg gggtgcgcat cccgtcgggg accaccgacc tctacgtcgc gatcaccggc
      361 atcctgctcg ccggcgcggc gtacgtgccg gtcgaccacg acgacccaga cgagcgggcg
      421 cgagtcgtct tcggcgaggc cggggtcgcc gcgatcgtcg gcaccgacct cgtcatccga
      481 tccggcccca ccgcgcggcc cgacgcagac cccgacgagg ctccggagga ggaccccgag
      541 ctcaccgacg acgcctgggt catcttcacc tcgggctcga ccggcctgcc caagggcgtc
      601 gccgtcagcc accgcaacgc cgccgccttc gtcgacgccg agtcgcggat gttcctccag
      661 cagcgcccga tcggccccgg cgaccgggtc atggccggcc tgtccgtcgc gttcgacgcc
      721 agctgcgagg agatgtggct cgcctggcgg tacggcgcct gcctcgtgcc ggcaccgcgc
      781 tccctcgtgc gctcgggcat ggacctcggc ccgtggctgg tcgccaacga catcaccatc
      841 gtctctaccg tgcccaccct cgtcgccctc tggccggccg acgccctcgc cgacgtccgc
      901 ctgctcatcc tcggcggcga ggcgtgccct cccgagatcg gcgcgcgcct ggccaccgac
      961 acccgcgagg tctggaacac ctacggcccc accgaggcca ccgtcgtcgc ctgcggtgcc
     1021 cagctcgacg gccagccccc cgtgcgcatc ggcctcccgc tcgacggctg ggacctcgcc
     1081 gtcgtcgacg cgcagggcca gcgcgtgccc gacggcgagc cgggcgagct gatcatcggg
     1141 ggcgtgggtc tggcccgcta cctcgacccc gagaaggatg ccgcggtgta cgccccgatg
     1201 ccgggcctgg gctgggagcg cgcctaccgc agcggcgacg tcgtccgctt cgacggcgtg
     1261 ggtctggtct tccaggggcg tgccgacgac caggtcaagg tcggtggtcg acggatcgag
     1321 ctcggtgagg tcgacagcgc cctgctcggc ctccccgggg tcagcggagc cgccgctgcg
     1381 gtcaagcgca ccgaggcggg caaccggctg ctcgtcggct acgtcaccgc cgacgcgggc
     1441 ttcgacccca aggccgcggc cgagcagctg cgggcgtcga tgccggccgc cctcgtcccg
     1501 cgcatcgccg tcgtcgacac cctgcccacc cgcacctccg ggaagatcga ccgcgacgcc
     1561 ctgccgtggc cgccacccgg cgcgtcccgc ggaaccggtg ccacggcatc cggcaccccc
     1621 gacctcgagc tcgacggcac ggccgcctgg atcgccgggc actgggccgc catcctcggc
     1681 tcgccgccct ccgcccccga cgaggacttc ttcgacctcg gtggggggag cctcagcgca
     1741 gcccagctgg tctccaaact gcgcgaacgg catccggacg tcaccgtcgc cgacatctac
     1801 gagcaccccg tcctcgccga cctcgcgcag accctcgacg ccatggccgc gccgaccggc
     1861 cgcaccaacg cctcggtctc gccggtcccg cgcgacaccc aggtcgcgca ggtgctcggc
     1921 accgtcgtcg tgcggtcgat cggggcgctg cgctggctga cgtggatcgg gctcgggctg
     1981 ctcgtcgctc accgggtcgt cgatgcgccg tggctgccga ggatcgcgtg gggctgggtg
     2041 ctcaccgggt ggctgctgct catcaacccc ttcgggcggg tgctgctcgg tgccgccgct
     2101 gcgcgcctgg tgctgcgcgg ggtcggccct ggccggtacc cccgcggtgg ccgggtgcac
     2161 ctgcggctct ggctcgccga gcgactggtt gacgaactgg gggccacgaa cctgtcggcc
     2221 gcgccactcg ttcgcgtcta cgcgaaactg ctcggatgcc gggtgggtcg tcacgtcgac
     2281 ctccacagca tcccgcccgt caccggcctg ctcacgctcg gcaccgggtg ctccatcgag
     2341 cccgaggtcg acctcgcggg gcactggctc gacggcggcc acgtgtacat cggcgcgatg
     2401 gcggtcggtg aggaggcccg cgtcggggcg cgcagcaccc tgctgcccgg ggccgacatc
     2461 ggcgaccggg cggaggtcgc tcccgggtcg gccgtgttcg ggccggtgcc ggccgacgag
     2521 gcctggtcgg gtgcgcccgc cggcccgtcg ggtgcggccc gcggcccgtg gacgccggag
     2581 cgcccgccga accgtcgcgt ctgggtgctc gcctacgccg cgtcggccat cgggctggcg
     2641 ctggtgccgg tgctcgccgt gctcgccggc gtcaccgtcg cgctcccgga gctggccgcg
     2701 gccaccgacc tcgccgggct ggtgaccgcg gcgctgtggt gggtgcccgt cgccaccgcg
     2761 gtcggtcttg tcgtgctcgt gggtctggtg ctcggcacgg tgcggctgct ctcgatcggt
     2821 ctcgagccgg gccaccaccc cgtgcacggt ctgcgggcgt ggcaggcctg gtcggtgctg
     2881 cgggtgctcg acgaggcgcg cacctggctc ttcccgctct actcctcgag cctgacgccg
     2941 tggtggctgc gagccctcgg cgccgacatc ggccccgacg tcgaggcctc gaccgtgctc
     3001 atgctgccga agttcgtcac ggtgcgcgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnc cggcgacacc acccgtaccc atgcgccgcc gacgcggctg cgggtggccc
     3181 gcgccctcgt cgagctcgcc cggatcgtcg cggtctgggt gcacgtggcg ctcatcgtgc
     3241 tcagcgccgc ggcccttctc gcactggtcg acgccggggc gctgtgggcg atcgcgctcg
     3301 gcgggccggt gctgctcgtc gcgggcgccg tggccgtcgg ggtcaccacc gtcgccaagt
     3361 ggctgctggt cgggcggctg cgaccgtcag aacacccgct gtggagctcg ttcgtctggc
     3421 gcaacgagct cgccgacacc ttcgtcgagg tcgtggccgc cccgtggttc gcccgggccg
     3481 ccgtcggcac gtccgtgctc aacgtctggt tgcgaagcat gggggcccgg gtgggccgcg
     3541 gcgtgtggtg cgagacctac tggttgcccg aggccgacct catcgacctg cgcgacgggg
     3601 cgacagtggg gcgcggctgc gtggtccaga cccacctgtt ccatgacagg atcctcgcga
     3661 tggaccgggt gacgatcgca gacgggggca cactgggccc caactccgtc atcctcccgg
     3721 ccgcccgcat cgggcgccac gccaccgtcg ggccggtctc gctcgtcatg cgcggggagt
     3781 cggtgcccga caagacccgg tggatcggca accccgtcgg cccatgggct gagcccgaat
     3841 gaccggctcc gacccctacc tgcccggcca cggcgacgac cggtaccagg tcgagcacta
     3901 cgccctcgac ctgcgctaca agccgctcgg caaccacctc gaaggccgtg ccgagctgtc
     3961 ggtgcgagcc gtggtcgacc tcaccgacct cgtcgtcgac ctgcaccggc tcgaggtgcg
     4021 cagcctgcgc gtcgagggag ccaaggtggc ccgctggtcg caccggggct cgcggctgcg
     4081 catccggctc acccaggaac tgccggctgg tgaaccgctg ctgctcacca tcgcctaccg
     4141 gggcaacccc ggcacgatgc ccggccccga cggaccggcc ggctgggaag agctcaccga
     4201 cggcgtgctc gtcgccgccc aaccgcacgg cgccccgacg tggttcccct gcaacgaccg
     4261 gatggccgac aaggcccgct accgcatccg ggtcgagacc gaccccgcct accgggtcgt
     4321 cgcgaacggc atcctcgtca gccacaagac cgtggggcgc cgcgccgagt gggtgcacga
     4381 gcagcgcgaa cccatggccc cgtacctcgc gaccctccag atcggccgct acgacgccgt
     4441 gcagcagacg ggcgctccgg tgccgacgtg gctcgtcggg ccgccggcgc gcatgcagtc
     4501 ggtgcgtgag gcgctcgccg accagccgcg catgctcgag gtgttcaccg agctgttcgg
     4561 cccgtacccg ttcgagcgct acaccgccgt cgtcaccgac gacccgctgg agatcccgct
     4621 cgagtcgcag tcgctgtcga cgttcggcac caaccacctc agccgggact ggtcggcgca
     4681 gcggctcatc gcgcacgagc tgtcgcacca gtggttcggc aacgccgtca ccgccgcctc
     4741 gctggccgac atctggctgc acgaggggtt cgcctgctac gccgagtggt tgtggtcgca
     4801 ggcgtccggg cgcacgacgc tcaggcagga ggccgcccgc caccacgacg ggctcgcccg
     4861 caagccccag gacctcgtgc tcaccgaccc cggagccgac cgcgtcttcg acgaccgggt
     4921 ctacaagcgg ggggcgctga cgttgtttgc cctgcacacg gctcttggcg acgagatgtt
     4981 cttcgacgtg ctgcgcacct gggtcgacac ccaccgctac gaggtcgtca tgaccgccga
     5041 cttcgaggca tgcgtcgagt cggtcagcgg ctcgccgcac cgcgagctgt tcgacgcgtg
     5101 gctgcgtcgc gaggaacttc ccgagctgcc accgcagcga gggtgacgac cacgtcggga
     5161 ccggcgcgcc accgcccctg cagacgcaac ggcatccgga tgccgtcact cgcctccgcg
     5221 tggaaggtgc cgtcggcggc gaggtggacc cggacctgct cgtggtgcag caccaccccg
     5281 gtcagcgggt accacgcctt gtaggtggcc tccttggcgg cgaagagcag ggtgtcccag
     5341 gggaggtcgg cgcggctgcg gcccaagtgc tccagcgcgg cacgctcggc tggggtcgcg
     5401 acgacgtcga gcacaccctc gggcaggggc accgccacct cggcgtcgag gccgatcgag
     5461 gcgaggccgg tgcgatgccc ccgcacgccc gtgccctgtc gggccgcgac cgcaccggtc
     5521 cagcccgccg tgtggctgat gctgccgatg actccggcgg gccagagggg cgcgccgttg
     5581 gcgtcggcca ggatcgggtg gtcaggcgcc tcggccccga tctcgcgcag ggcggttcgt
     5641 gcgcactcgc gtcccgtggc gaactcggca cggcgttgcg gttgggcgca gtcgacgagg
     5701 gccgcctcgg ccgggtgcag ggtggcgggg tcggccgcca cggtcgtcac gccccacccg
     5761 atcc
//
LOCUS       DAOSOC010000159         5695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_14321_length_5695_cov_9.508333, whole genome shotgun sequence.
ACCESSION   DAOSOC010000159 DAOSOC010000000
VERSION     DAOSOC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14321_length_5695_cov_9.508333"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PL014_10725"
     CDS             <1..434
                     /locus_tag="PL014_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HOT57606.1"
                     /translation="FCLGWAAGKAQGLGQRVLVTAVAIPTVGTFSGNPKRDLLTLTAA
                     LLVIWVPTLRVPRGLVPLVQVLAASSLFIYVLHWHVLQETRSTPWLGYLGGLVAGVAY
                     WWVWTRGPGLARAARGRWARGTRPAAPRHADQPMTSAASSH"
     gene            complement(401..2305)
                     /locus_tag="PL014_10730"
     CDS             complement(401..2305)
                     /locus_tag="PL014_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT57607.1"
                     /translation="MANLVSVERASLALGTAQVLDAVSLGVGGGDRIGIVGRNGGGKT
                     TLLRVLSGTRDVDTGRVARVGGLTVGVLTQDDGLDPAATVHETVIGDLPEHEWAASAR
                     IRDVLTGLLGGLDAPAVGGMAAAVGPLSGGERRRVALAALLVSAPDLLLLDEPTNHLD
                     VEGVAWLADHLVRQHARPDNAVVAITHDRWFLDAIATQTWEVDSGQVFAYEGGYAAYV
                     LAKAERERMAGVIAERRDNLLRKELAWLRRGPPARTSKPKFRIDAANALIADEPPARD
                     GVELMRFATTRLGKDVVDLIDARISVGDRVLLDHVTWRLAPGERTGIVGVNGAGKSTL
                     LRAIAGDVALAAGKRKVGVTVRLAVLSQEVRELEKWADRRVIEAVEDVRAVVRFGAKE
                     LTASQLAQRLGFSGPRQQTRVGDLSGGERRRLQLTRLLMDEPNVLLLDEPTNDLDIET
                     LTSLEDLLDGWAGTLIVVSHDRYLLERVCDRQVALLGDGRLRELPGGVEEYLRLRAAA
                     AGGPASTWDAGRSAAAAVATAAAVSASAGPGAAGTAGGGQAASAKPATPTAPAVSPAE
                     AREARKTMARVEKQLSRLADREEKLHAAMLTAATDHARVLVLNRELREIVDEREDLEL
                     QWLEAAEVIG"
     gene            complement(2305..3234)
                     /locus_tag="PL014_10735"
     CDS             complement(2305..3234)
                     /locus_tag="PL014_10735"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT57608.1"
                     /translation="MTSAPLVPAPVTVRVPAKVNLELLVGPRRPDGYHALSTVFQAVS
                     LYDDVTVAAAEDWGVTVSGPLAAGVPEDGDNLAMRAARLVEAHFDIDPVHIAIRKGIP
                     VAGGMAGGSADAAATLVALDHLWDLDLDREELEDLASELGSDVPFLIAGGTAMGSGRG
                     ELLAPVLARGTYHWVFALAEGGLSTPAVYAECDRLRADEDVPDPVPTAALMSALRTGD
                     PHELAAQLSNDLQAASLSLRPDLAEVLAAGMEYGALGGIVSGSGPTTAFLVENNEAGI
                     DLAVALTASGVVRDVRRASGPVHGAHVIHQRTD"
     gene            complement(3254..>3763)
                     /locus_tag="PL014_10740"
     CDS             complement(3254..>3763)
                     /locus_tag="PL014_10740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HOT57609.1"
                     /translation="PTALVANLPYNVSVPVVLRFLELFPTVRTILVMVQLEVAERLAA
                     GPGSKTYGVPSVKAAWYADVRLAGRVPRSVFWPVPNVDSGLVALTRREPPVTDVTRQE
                     VFACVDAAFAQRRKTLRAALASWAGSPALAEAALTAAGVDPRTRGEQLDVATFAAVAS
                     ARVQAVQGP"
     assembly_gap    3764..3863
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3864..4003)
                     /locus_tag="PL014_10745"
     CDS             complement(<3864..4003)
                     /locus_tag="PL014_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HOT57610.1"
                     /translation="MSDDHEVSLLGAGEIRDLAARHGIRPTKQWGQNFVVDGNTVRRI
                     VRV"
     gene            complement(4086..5228)
                     /locus_tag="PL014_10750"
     CDS             complement(4086..5228)
                     /locus_tag="PL014_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HOT57611.1"
                     /translation="MLFTRPLRHVAQGVAALVIAGGAVGIAHADKAVEVTVDGQPTSV
                     HVFGSTVADVLAKQDITVGPHDDVVPSLDTPVSDGDRISVRYGRLLTVTVDGETTERW
                     TTATTVDGALTELNIRADGAALTASRSQSLGRHGLTLAVTTPKNVTVVADGKTRKVTT
                     TSQTVFGVLAELDVAKRTADVVSPVMPTSVEDGMKITLKRVDVKTETAKQAIAFTTTK
                     QKDSSLYKGQTKVVTKGKKGAKTVVSQVTYVDGKKTSTKVVKSTVTAKPVAAVVKVGT
                     KARPAATTSTGGSGGGSTSGAGINTARSAMWDRIAQCESGGRWNINTGNGYYGGLQFA
                     TASWLAMGGDDFAPRADLASRAEQITIANRYYARAGLSPWGCAHAA"
     gene            complement(5511..>5695)
                     /locus_tag="PL014_10755"
     CDS             complement(5511..>5695)
                     /locus_tag="PL014_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT57612.1"
                     /translation="DAPYLTPHPWRGATNSPALVPLTVRVMAGVLGVAVPTLCEALSD
                     NAERLYGPWSAPFATP"
BASE COUNT          739 a         1952 c         2098 g          806 t
ORIGIN      
        1 tgttctgcct gggctgggcc gctggcaagg cgcaggggct gggtcagcgg gtgctcgtca
       61 cggccgtcgc gatccccacc gtcggcacct tctcgggcaa ccccaagcgt gacctgctca
      121 ccctcaccgc ggccctgctc gtcatctggg tgccgaccct gcgagtgccc cgtggcctcg
      181 tgccgctggt gcaggtgctc gccgcgagct cgctgttcat ctacgtgctg cactggcacg
      241 tgctccagga gacccgctcg accccgtggc tcggctacct gggtggcctc gtggccggtg
      301 tcgcctactg gtgggtgtgg acccgcggcc ccggcctggc gcgcgccgct cgtggcaggt
      361 gggcgcgggg cactcggccg gccgcgcccc gccacgcaga tcagccgatg acctcggcgg
      421 cctccagcca ctgaagctcg aggtcctcgc gctcgtcgac gatctcgcgc agctcgcggt
      481 tgagcacgag cacccgggcg tggtcggtgg ctgccgtgag catcgcggcg tgcagcttct
      541 cctcgcggtc ggcgaggcgg ctcagctgct tctcgacccg ggccatcgtc ttgcgggcct
      601 cacgggcctc ggccggcgac accgccggtg ccgtgggcgt cgcaggcttg gcggatgccg
      661 cctgcccacc acccgctgtc cctgcagccc ccggcccggc cgacgccgag accgcggccg
      721 ccgtcgcgac ggcagcggca gccgagcgac cggcatccca ggtcgaggcc ggaccaccgg
      781 ccgcggcggc gcgcaggcga aggtactcct cgacgccccc gggcagctcg cgcaggcggc
      841 cgtcaccgag cagcgcaacc tgtcggtcac acacgcgctc gaggaggtag cggtcgtggg
      901 agacgacgat gagggtgccc gcccacccgt cgaggaggtc ttccagggag gtcagcgtct
      961 cgatgtcgag gtcgttggtc ggctcgtcga gcagcagcac gttcggctcg tccatgagga
     1021 ggcgggtcag ctgcaggcgc cgccgctcac caccggagag gtcgccgacg cgcgtctgct
     1081 ggcggggccc gctgaacccg agccgctgcg cgagctggga tgccgtgagc tccttggcgc
     1141 cgaagcgcac caccgcgcgc acgtcctcga cggcctcgat gacccgtcgg tcggcccact
     1201 tctcgagctc gcgaacctcc tgcgagagca ccgccaggcg cacggtgaca ccgaccttgc
     1261 gcttgcctgc ggctagcgcg acgtcccccg cgatggcgcg cagcaacgtc gacttgccgg
     1321 cgccgttgac gccgacgatg ccggtgcgct cgccgggggc caggcgccag gtgacgtggt
     1381 cgagcagcac ccggtcgccg acgctgatcc gggcgtcgat gaggtcgacg acgtccttgc
     1441 cgagacgggt cgtggcgaac cgcatgagct cgacgccgtc acgggccggg ggctcgtcgg
     1501 cgatgagcgc gttggccgcg tcgatccgga acttgggctt gctcgtgcgg gccggcgggc
     1561 cgcgccgcaa ccaggcgagc tccttgcgaa ggaggttgtc acggcgctcg gcgatcaccc
     1621 cggccatccg ctcgcgttcg gccttggcca gcacgtaggc cgcgtagccg ccctcgtagg
     1681 cgaagacctg gccggagtcg acctcccagg tctgcgtggc gatcgcgtcg aggaaccagc
     1741 ggtcgtgggt gatcgcgacg acggcgttgt ccggccgggc gtgctggcgc acgaggtggt
     1801 cggccagcca ggccacccct tcgacgtcga ggtggttggt cggctcgtcg agcagcagca
     1861 ggtcgggcgc ggagacgagc agcgccgcca gcgccacccg ccggcgctcg ccaccggaca
     1921 acggccccac cgcggcagcc atcccaccga cggcgggtgc gtcgagccca ccgagcaacc
     1981 cggtgaggac gtcgcggatg cgtgcgctgg cggcccactc gtgctcgggg aggtcaccga
     2041 tgacggtctc gtgaacggtg gctgccgggt cgaggccgtc gtcctgggtc agcaccccca
     2101 cggtcagccc gcccacgcga gccacccggc cggtgtcgac gtcccgggtg cccgacagca
     2161 cccgcaggag ggtggtcttg ccaccgccgt tgcgaccgac gatgccgatg cggtcgccgc
     2221 ccccgacccc gagggagacg gcatcgagca cctgggcggt gccgagcgcg agggaggccc
     2281 gctcgaccga gacgaggttg gccactagtc ggtccgctgg tggatcacgt gggcaccgtg
     2341 gaccggaccg ctggcgcgcc gcacgtcgcg gacgacgccg ctggccgtga gggcgacggc
     2401 gaggtcgatg ccggcctcgt tgttctcgac gaggaaggcc gtcgtcgggc cggagccgga
     2461 gacgatgccg cccagcgccc cgtactccat gcccgcggcc agcacctcgg cgaggtcggg
     2521 acgcagcgac agtgacgccg cctggaggtc gttggacagc tgtgcggcga gctcgtgggg
     2581 gtcgccggtg cgcagggcgc tcatgagggc cgcggtggga acggggtcgg gcacgtcctc
     2641 gtcggcacgc aggcggtcgc actcggcgta gacggccggg gtggacagac cgccctcggc
     2701 gagcgcgaac acccagtggt aggtgccccg ggcgagcacg ggggcgagca gttcgccgcg
     2761 cccgctgccc atcgccgtgc ccccggcgat gaggaagggc acgtcgctgc cgagctcgct
     2821 ggcgaggtcc tccagctctt cgcggtccag gtcgaggtcc cagaggtggt cgagcgcgac
     2881 cagggtggcc gcggcatccg ccgacccgcc ggccatgccg cccgccaccg ggatgccctt
     2941 gcggatggcg atgtgcaccg ggtcgatgtc gaagtgggcc tccacgagcc gtgccgcgcg
     3001 catggcgagg ttgtcgccgt cctcgggcac ccccgccgcg agtggcccgg agacggtgac
     3061 accccagtct tcggcggcgg ccacggtgac gtcgtcgtag aggctcaccg cctggaacac
     3121 cgtggacagt gcgtggtagc cgtcggggcg gcgcgggccg acgagcagct cgaggttgac
     3181 cttggccggc acccggaccg tcaccggggc ggggacgagg ggagccgagg tcatgtcccc
     3241 accctaaagt gcttcagggc ccctgcaccg cctgaacccg agcggaggcg accgcggcga
     3301 aggttgcgac gtcgagctgc tccccacggg tgcgggggtc aactcccgcc gccgtcaggg
     3361 cggcctcggc cagcgcgggt gaccccgccc acgaggcgag ggcagcgcgc agggtcttgc
     3421 ggcgctgcgc gaaggccgcg tcgacgcagg cgaagacctc ctgccgggtg acgtcggtga
     3481 ccggtggttc gcggcgggtc agggcgacga gtccggagtc gacgttcggc accggccaga
     3541 agacgctgcg cggtacccgg ccggcgagcc gcacgtcggc gtaccaggcg gccttgacgc
     3601 tcggcacccc gtacgtcttg gaaccagggc ctgccgcgag ccgctcggca acctcgagct
     3661 ggaccatgac gaggatcgtg cgcaccgtgg ggaacagctc gaggaagcgc aggaccaccg
     3721 gcacggacac gttgtagggc aggttcgcga cgagggccgt cggnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnacccgca cgatgcgccg gacggtgttg ccgtcgacga
     3901 cgaagttctg cccccactgc ttggtcgggc ggatgccgtg gcgcgccgcg aggtcgcgga
     3961 tctcacctgc cccgagcagg ctcacctcgt ggtcgtcgga catgcgctca ccctagccac
     4021 gacgcacggt gccgggccga cgcgaggcgt cgacccggca tccgaggtcg tgatcaggtc
     4081 gttgatcagg cggcgtgcgc gcagccccac ggggagaggc cggcccgcgc gtagtagcgg
     4141 ttggcgatgg tgatctgctc ggcgcgcgat gcgaggtcgg cgcggggggc gaagtcgtcg
     4201 ccacccatgg ccagccagga cgcggtggcg aactgcaggc cgccgtagta gccgttgccg
     4261 gtgttgatgt tccagcggcc accggactcg cactgggcga tccggtccca catggcggag
     4321 cgggcggtgt tgatgcccgc gccagacgtg ctgccgccgc cgctgccacc ggtgctcgtg
     4381 gtggccgccg ggcgggcctt ggtgccgacc ttgacgaccg ccgcgacggg cttggcggtg
     4441 accgtgctct tgacgacctt ggtgctcgtc ttcttgccgt cgacgtaggt gacctggctg
     4501 acgacggtct tggcgccctt cttccccttg gtgacgacct tggtctggcc cttgtacagg
     4561 ctcgagtcct tctgcttggt cgtcgtgaac gcgatggcct gcttcgcggt ctcggtcttg
     4621 acgtccacgc gcttcagggt gatcttcatg ccgtcctcga ccgaggtcgg catgaccggg
     4681 gacacgacgt ccgccgtgcg cttggcgacg tcgagctcgg ccaggacgcc gaacacggtc
     4741 tggctcgtcg tggtgacctt gcgggtcttg ccgtcggcga cgaccgtgac gttcttcggc
     4801 gtggtgaccg ccagggtgag gccgtgacgg cccaggctct gcgaacgcga ggccgtcagg
     4861 gccgcaccgt cggcgcggat gttcagctcg gtcagggcgc cgtcaaccgt ggtggccgtg
     4921 gtccagcgct cggtcgtctc accgtcgacg gtcaccgtga ggaggcggcc gtagcgcacc
     4981 gagatgcggt cgccgtcgct gaccggggtg tccagggagg gcaccacgtc gtcgtgcggg
     5041 ccgacggtga tgtcctgctt ggcaaggacg tcggcgacgg tgctgccgaa gacgtgcacc
     5101 gaggtgggct gaccgtcgac ggtgacctcg actgccttgt ccgcgtgggc gatgccgacg
     5161 gcaccgccag cgatgacgag ggcagcgaca ccttgggcga catgacgaag ggggcgggtg
     5221 aaaagcacgg ttctccagtg ttcgtgatct ccgggcaccg atctcggacg cggccgcagg
     5281 cggccctgac gacccgtcgc cggggcgtcg cacgcagaca ccttccctgc cgacaccggc
     5341 ggcgaacagg gtggtcgtac gtgccgtccg aggcgtttcg gccccggcca ttccctccag
     5401 cgtgacggag tcagcgggtc aaggtcaaat tttggtaacg gtcatgcagg gctcggcgcg
     5461 cctcacgcag cacatctgtc gcaccagccc caccagtaac gagatctgcg tcatggtgtg
     5521 gcgaacggcg ctgaccacgg gccgtagaga cgctcggcgt tgtcgctgag ggcctcgcac
     5581 agggtgggca ccgcgacgcc gaggaccccg gccatcaccc gcaccgtgag gggcaccagg
     5641 gccggtgagt tcgtcgcgcc gcgccacggg tgcggcgtga ggtagggcgc gtcgg
//
LOCUS       DAOSOC010000160         5644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_14486_length_5644_cov_8.932904, whole genome shotgun sequence.
ACCESSION   DAOSOC010000160 DAOSOC010000000
VERSION     DAOSOC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14486_length_5644_cov_8.932904"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..822)
                     /locus_tag="PL014_10760"
     CDS             complement(43..822)
                     /locus_tag="PL014_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57613.1"
                     /translation="MLDHGPLGGDSGWRAALLHVGTSARLGGVTALQASGLTGFEETA
                     IHIWVTKSTRKGRPAGVVLHESRRWGDDDAAPHGIPRARPAVAAVQAALWARTPRQAA
                     YLLVLPVQQRLVSVEDLGVEMSRVRRHAYRGVLSGVVADIGDGVRSMNELDFARLCRA
                     RGLPEPSRQTLRESAQGRRYLDVEWVAYDVRLEVQGAGHARLLNVLSDDVRLMDLAAE
                     DGAAVSVSVLSLRVDPEPLFASLARLLQSRGWRGAYRPPSP"
     gene            complement(1217..1702)
                     /locus_tag="PL014_10765"
     CDS             complement(1217..1702)
                     /locus_tag="PL014_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT57614.1"
                     /translation="MPRILRGDDVLNTTFTQTQFRRGYDEREVDDFLDRAVVALRHHE
                     SGRPVAEAPMSSRDVEGGRFTQTQFRRGYDEEEVDTLLADLAGTLAQHEAGGSAAGSA
                     AEAAAAAAAAVDGSAVPGSAGSGAARSARPAGIPYAGGILIEEESWGSKLLRKLRGDK
                     G"
     gene            complement(1702..5601)
                     /gene="purL"
                     /locus_tag="PL014_10770"
                     /gene_synonym="purI"
     CDS             complement(1702..5601)
                     /gene="purL"
                     /locus_tag="PL014_10770"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOT57615.1"
                     /translation="MASSHDLVLTTVPGGNALSPFRAAALLERLHRAVPTVTGVSARH
                     VHWVASEQPLDEATAATVTKLLTYGPPDAGAASGAHLATVVVAPRLGTLSPWASKATD
                     IAHNCGVDIRRVERVTEYLVATDEPLTEEQWGALADLLHDRMTEAALPTRADAAALFD
                     EREAEPMERVDVLGRGREAIVEADTTYGLALSDDEVDYLVDAFTGLGRNPSDVELMMF
                     AQANSEHCRHKIFNADFVVDGEVMPRSLFGMIRHTEAVAGEGTVVAYKDNASVMVGGR
                     RVRWMPEAADGPSRYAGREGEVHVLMKVETHNHPTAISPFPGAATGAGGEIRDEGATG
                     RGSAPKAGLTGFAVSNLHLPGLEEPWESEQYGAPAHIASPLEIMLDGPIGAAAFNNEF
                     GRPGLGGFFRVYEQTVDGVRRGFHKPIMSAGGLGTISADMTEKIVFPAGTLLVQIGGP
                     GMRIGMGGGAASSMAAGANAAELDFDSVQRGNPEIERRAQEVINHCWGLGDDNPVLAI
                     HDVGAGGLSNAFPELVDGAGLGARFDLSAVPLEESGLAPKEAWCNESQERYVLALAPD
                     SLPLFASLCERERCPYAVVGVARDDGRLVLADGPDDLDDEDRAIDMPMEVLLGKPPKM
                     LRDVTRVERDPGTLDLTGLDLKDAAYAVLRHPTVASKRFLVTIADRTVGGLTHRDQMV
                     GPWQVPVADVAVTLADHVGFAGEAMATGERMPVASVDAPASGRMAVGEALTNLLAAPL
                     SSLTGVKLSCNWMAACGEDGEDAALFDTVEAVAMQLLPELGVSVPVGKDSLSMRTRWT
                     DAATGEARQVTSPVSLVVTAFASLPDVRGTWTPQLRPGDVLVLVDLGAGRDRLGGSIL
                     GQVQGEFGGPVPDLDDPQLLLSLVAALADLRSRSVDVAYHDRSDGGLWAAVCEMAFAG
                     AVGVELDVPSVAALFAEELGAVLGVPATRTAEVLQVFASHGLGDVVTVVGGTASDRVV
                     RVSVAGEAAIQEPVRDLAQAWDEVSWRIATLRDNPECAAQEHEAFGADGDPGLVVAPT
                     FDPTDDVAAPYVNLGARPKVAILREQGVNSHVETAFAFDRAGFDTYDVHMTDLQAGRF
                     DLADVVGLVACGGFSYGDTLGAGEGWARSVLFNEALTQGFHDFFHRGDTFGLGICNGC
                     QMFAALADLIPGADAWPRFTRNVSEQYEARLSMVEVVESPSIFTAGMAGSRLPIAVAH
                     GEGFADFSSRGDADAVARVARFVDSHGAVASRYPANPNGSPDGLTAVTTPDGRFTAMM
                     PHPERVARNAQLSWTSEPVEAESPWLRMFRNARVWVD"
BASE COUNT          864 a         2081 c         1962 g          737 t
ORIGIN      
        1 cgtccggcac cttggctgcc agaggcacca acctgccgga cgctacgggc tgggcggacg
       61 gtaggcccct cgccagccac gtgactgcaa caggcgcgcc aagctcgcga acagcggctc
      121 agggtcgacc cgcagcgaca gcacggacac actcacggcc gcaccgtcct cggcagcgag
      181 gtccatgagg cggacgtcat cactgaggac gttgagcaga cgcgcgtgcc cggcaccctg
      241 aacctcaaga cgaacgtcgt acgccaccca ctcgacgtcg aggtatctgc gcccctgggc
      301 cgactcacgc agggtctggc ggcttggctc cgggagacca cgcgcgcgac acagccgggc
      361 aaagtccagt tcgttcatgg agcgcacgcc gtccccgatg tcagcgacga ctccggagag
      421 gaccccccgg taggcgtggc gccgaacgcg agacatctcg actcccagat cttccacact
      481 caccaaccgc tgctgaaccg gcaacaccag caggtacgcc gcctgtcgag gggttcgtgc
      541 ccacaatgcc gcctgcaccg cagccaccgc gggacgggcg cgcgggatgc cgtgaggcgc
      601 cgcgtcgtcg tcgccccacc gtcggctctc atgcagaacg acgccggctg gacgcccttt
      661 gcgggtggac ttcgtgaccc agatgtggat ggcggtctcc tcgaacccgg tcagcccgga
      721 tgcctggagt gcagtgaccc cacccagccg ggccgacgta ccgacgtgga gcagggccgc
      781 gcgccacccg gaatccccgc cgagcggccc gtggtcgagc acgacaccac ggtgcggcag
      841 gtgccgccaa cgctcggcgg cgacatgcgc acgtcggcgt cctcgggtca cccctgcctc
      901 tcggagacga gccaaggaca cgacacgatc gatggcctcg gggagctcgc cgacgtctcg
      961 gaggcggcgc gactgatggt ccatggctcg atggtcagac cccgggtcga tccggcacag
     1021 ccagcgggag ggggctgtgg acaacgcgag cagtccgcgg gccctgtgga caacggcatc
     1081 cagcccgttg cgtccggcaa gttggctgtc agaggaacca acctgccgga cgcaagaaat
     1141 cacgcttggc caacgtccgg cactttggct gccgggggca ccaacctgcc ggacgcaagc
     1201 cgaaaaggcc tgggggtcag cccttgtcgc cgcgcagctt tcttagcagc ttggagcccc
     1261 aggactcctc ctcgatcagg atgccgccgg catacgggat acccgcgggg cgtgcactcc
     1321 gagcggcccc tgagcccgcc gaaccaggca cggccgatcc atccaccgca gccgcagccg
     1381 cagccgctgc ctctgccgcc gagccagctg ctgagccacc ggcctcatgc tgtgcaagcg
     1441 tgcccgcgag gtcggcgagc agcgtgtcga cctcctcctc gtcataaccc cggcggaact
     1501 gcgtctgggt gaaccgccca ccctccacgt cgcgggacga catcggcgcc tcagcgacgg
     1561 gccggcccga ctcgtggtgc cgcagggcga cgaccgcccg atcgaggaag tcatcgacct
     1621 cccgctcgtc gtacccccgc cggaactgcg tctgggtgaa ggtggtgttg aggacgtcgt
     1681 cccctcgcag gatcctgggc atcagtccac ccacacccgc gcgttgcgga acatccgcag
     1741 ccacgggctc tcggcctcga ccggctccga ggtccacgac agctgcgcgt tgcgggcgac
     1801 ccgctcgggg tgcggcatca tcgcggtgaa ccggccgtcg ggcgtcgtca ccgcggtcaa
     1861 gccgtcgggc gacccgttgg ggttggccgg gtaccgcgaa gccaccgcac cgtgcgagtc
     1921 gacgaaccgc gcgacgcgcg ccacggcatc cgcgtcaccg cgtgacgaga agtcggcgaa
     1981 gccctcaccg tgggcgacgg cgatcggcaa ccggctgccc gccatgccgg cggtgaagat
     2041 cgacggcgac tccacgacct cgaccatcga cagccgcgcc tcgtactgct cggagacgtt
     2101 gcgggtgaac cgcggccacg cgtcggcgcc cgggatcagg tcggccagcg ccgcgaacat
     2161 ctggcagccg ttgcagatgc cgagcccgaa ggtgtcgccg cggtggaaga agtcgtggaa
     2221 gccctgcgtc aacgcctcgt tgaacagcac cgagcgcgcc cagccctcac cggcgccgag
     2281 ggtgtcgccg tacgagaacc cgccgcacgc caccagcccg acgacgtcgg cgaggtcgaa
     2341 ccgccccgcc tggaggtcgg tcatgtgcac gtcgtaggtg tcgaacccgg cccggtcgaa
     2401 cgcgaacgcg gtctcgacgt ggctgttcac gccctgctcg cgcaggatgg ccaccttcgg
     2461 ccgcgccccg aggttcacat agggcgccgc cacgtcgtcg gtggggtcga aggtcggcgc
     2521 gacgacgagc ccggggtcgc catcggcccc gaacgcctcg tgctcctgcg cggcgcactc
     2581 cgggttgtcg cgcagcgtcg cgatgcgcca cgacacctcg tcccaggcct gtgcgaggtc
     2641 acgcaccggc tcctggatcg ccgcctcacc ggccaccgac acgcgaacga cccgatcgga
     2701 tgccgtcccg cccaccaccg tgaccacgtc accgaggccg tggctggcga acacctggag
     2761 cacctcggcg gtgcgggtgg cgggcacccc gagcacggca ccgagctctt cggcgaacag
     2821 cgcagccacc gacggcacgt cgagctcgac ccccacggca ccggcaaaag ccatctcgca
     2881 caccgcagcc cacaggccac cgtcggaccg gtcgtggtac gccacgtcga ccgagcggga
     2941 ccgcaggtcg gccagcgccg ccacgagtga gagcagcagc tgcgggtcgt cgaggtccgg
     3001 aaccgggcca ccgaactcac cctgcacctg ccccaggatc gacccgccca gccggtcgcg
     3061 accagcgccg aggtcgacga gcacgagcac gtcgcccggg cgcagctgcg gggtccacgt
     3121 gccacgcacg tcgggcagcg aggcgaacgc cgtcacgacg agcgacaccg gcgaggtgac
     3181 ctggcgcgcc tccccggtcg cggcatccgt ccagcgggtg cgcatcgaca gcgagtcctt
     3241 gccgaccggc accgacaccc cgagctcggg gagcagctgc atcgcgacgg cctcgacggt
     3301 gtcgaacagc gcagcgtcct cgccgtcctc accgcaggcg gccatccagt tgcacgacaa
     3361 cttgaccccg gtcaacgacg acagcggcgc tgcgagcagg ttggtcagcg cttccccgac
     3421 cgccatccga cccgacgcgg gagcgtcgac cgaggccacc ggcatccgct cgcccgtggc
     3481 catcgcctca cccgcgaagc cgacgtggtc ggccagggtc acagcgacgt cggcgacggg
     3541 cacctgccac ggcccgacca tctggtcgcg gtgggtcagc ccgccgaccg tgcggtcggc
     3601 gatcgtcacg aggaaccgct tggacgcgac cgtcggatgc cgcagcacgg catacgcggc
     3661 gtccttcagg tccagcccgg tgaggtccag ggtgcccggg tcgcgctcca cgcgggtcac
     3721 gtcgcgcagc atcttcggtg gcttgccgag cagcacctcc atcggcatgt cgatggcgcg
     3781 gtcctcgtcg tcgaggtcgt ccgggccgtc ggcgagcacg aggcggccgt cgtcgcgagc
     3841 gaccccgacg acggcgtagg ggcagcgctc acgctcgcac agcgacgcga acagcggcaa
     3901 tgaatcgggc gcgagcgcca gcacgtagcg ctcctgcgac tcgttgcacc aggcctcctt
     3961 gggggcgagc cccgactcct cgagcggcac ggccgacagg tcgaagcggg cccccagccc
     4021 ggccccgtcg acgagctcgg ggaaggcgtt cgacaggccg ccggcgccga cgtcgtggat
     4081 ggcgagcacg gggttgtcgt ccccgaggcc ccagcagtgg ttgatgacct cctgggcgcg
     4141 gcgctcgatc tcggggttgc cgcgctgcac ggagtcgaag tcgagctcgg ccgcgttggc
     4201 cccggccgcc atcgacgacg cggccccacc gcccatgccg atgcgcatgc cgggcccgcc
     4261 gatctgcacg agcaacgtgc ccgcggggaa gacgatcttc tcggtcatgt cggcgctgat
     4321 cgtgccgagg ccgccggcgc tcatgatcgg cttgtggaag ccgcggcgca ccccgtcgac
     4381 ggtctgctcg tagacccgga agaacccgcc cagcccgggc cggccgaact cgttgttgaa
     4441 ggctgccgcc ccgatcgggc cgtcgagcat gatctccagc gggctcgcga tgtgcgccgg
     4501 ggcaccgtac tgctcgctct cccagggctc ctcgagaccg ggcaggtgca ggttggacac
     4561 ggcgaacccg gtgagcccgg ccttcggcgc cgatccccgg ccggtggcgc cctcgtcgcg
     4621 gatctcgccg ccggcacccg tggccgcacc cgggaagggg ctgatcgcgg tcgggtggtt
     4681 gtgcgtctcg accttcatga gcacgtgaac ctcgccctcg cgcccggcgt agcggctcgg
     4741 cccgtcggcg gcctccggca tccagcgcac gcgacgtccg ccgaccatga ccgaggcgtt
     4801 gtccttgtag gcgacgaccg tgccctcacc ggcgacggcc tcggtgtgcc ggatcatccc
     4861 gaacagcgag cgcggcatca cctcgccgtc gacgacgaag tcggcgttga agatcttgtg
     4921 gcggcagtgc tcggagttgg cctgcgcgaa catcatcagc tcgacgtcgc tggggttgcg
     4981 cccgagcccg gtgaacgcgt cgacgaggta gtcgacctcg tcgtcggaca gggccaggcc
     5041 gtaggtcgtg tcggcctcga cgatcgcctc gcggccgcgt ccgagcacgt cgacgcgctc
     5101 catcggctcg gcctcgcgct cgtcgaacag cgcggcggcg tcggcccggg tgggcagggc
     5161 agcttcggtc atccggtcgt ggaggaggtc tgcgagggcg ccccactgct cctcggtgag
     5221 cggctcgtcg gtggcgacga ggtactcggt gacgcgctcc acccggcgga tgtcgacgcc
     5281 gcagttgtgc gcgatgtcgg tggccttgct cgcccacggc gacagcgtcc ccaggcgcgg
     5341 ggcgacgacc acggtggcga ggtgggcacc cgaggcggca ccggcatccg gcggcccgta
     5401 ggtcagcagc ttcgtgaccg tggccgcggt cgcctcgtcg aggggctgct cactggcgac
     5461 ccagtgcacg tgccgcgcgc tgacaccggt gaccgtcggg acggcgcggt gcaggcgttc
     5521 gagaagcgct gccgcacgga acggcgagag ggcgttgccg cccgggacgg tggtcagcac
     5581 gaggtcgtgc gacgaagcca tgagcaggtc tctcccggag ctgggcgcga gggcggatgc
     5641 cgtg
//
LOCUS       DAOSOC010000161         5591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_14641_length_5591_cov_10.663656, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000161 DAOSOC010000000
VERSION     DAOSOC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14641_length_5591_cov_10.663656"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="PL014_10775"
     CDS             complement(<1..125)
                     /locus_tag="PL014_10775"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851464.1"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT57616.1"
                     /translation="MDSRTTVEAMLRADLRGRTAYGAPQLDVPVALNTNENSYPVP"
     gene            complement(197..1537)
                     /gene="hisD"
                     /locus_tag="PL014_10780"
     CDS             complement(197..1537)
                     /gene="hisD"
                     /locus_tag="PL014_10780"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOT57617.1"
                     /translation="MPDTIIPRLDLRGQHLSLRELKAALPRAEFDVEAALDAVRPIVA
                     DVAERGAAALYDAAERFDGIRPARLRVPAEVIADALTTLDPAVRAALEESIRRARVVH
                     ADQRRSDTTTQVADGGTVTERWVPVDRVGLYVPGGLAVYPSSVVMNVVPAQLAQVPSL
                     AVASPPQRDHGGWPHPTILAACALLEVDEVWAMGGAQAVAAFAYGIEDDGEVCDPVSL
                     VTGPGNIYVAAAKRLLKGIIGIDAEAGPTEIAVLADDSADADHVASDLISQAEHDPLA
                     AAVLVTDSPDLVDAVEAALARRVATTKHTERVATALAGRQSAIVLVDDVEAGLALVNA
                     YAAEHLEIQTRDAASVAARVRNAGAVFVGPWSPVSLGDYAAGSNHVLPTGGCACHSSG
                     LSVQSFLRGIHVVTYSSEALREVGGHVVTLARAEDLPAHGEAVLARHPELDPSA"
     gene            complement(1561..4557)
                     /locus_tag="PL014_10785"
     CDS             complement(1561..4557)
                     /locus_tag="PL014_10785"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HOT57618.1"
                     /translation="MSSRGPGSIPVAIARLGFDDAARAFGLLQDPALAGLIHSREHIE
                     DHGLAAALAGVPDPDGALLGLVRFMEVVGRDPELRGPVTEALAAPGPARQRLLAVLGS
                     SAALGDHLCAHPHQWSAVTRAQPLTAQQRIQRLVGAVSAPEGAPAVDVLRTAYREQLL
                     EIAALDLTSGDPLVSLPETAAALADLAQAALEAALVIARDEVGADAGRTRLAVIAMGK
                     TGGRELNYISDVDVIFVAEPADGVPEEESTAIATDLATRLMRICSASTATGALWQVDP
                     ALRPEGKNGPLVRTVASHRSYYERWAKTWEFQALLKARPVAGDAEVGQAYCDAVQPMV
                     WQASTRENFVADVQAMRRRVEQHIPPAEAARQLKLGAGGLRDVEFSVQLLQLVHGRAD
                     ESLRTGTTLDGLAALSAGGYVGREDAATLATAYRLLRTLEHRIQLYRLRRTHLMPTAA
                     SDLRRLGRALGHRSSPADAVIAQWKAQQREVRRLHERIFYHPLLSAVARLSDSEVRLT
                     PEAARERLSALGFRDPHGALRHLEALTDGLSRRAAIQRQLLPVMLGWFADEADPDAGL
                     LAFRKISDELGTTPWYLRLLRDEGSAAERLAHTLARSRFAAGLLEQAPECVQFLGDAA
                     GLRPRAREDVLRRMRSAAGRKDDPASAVLAARTIRRSELFRVAVADLSGLLTLDGLGT
                     AMTDLTSALLEVTLELCVRDLEARTGAPALTRVVVVGMGRLGGGEQGYGSDADVLFVH
                     DPVDGADEGAAQAQALEVVKDLIRLLGLRGPDPNLDIDAGLRPEGKNGPLVRSLASYR
                     EYYARWSLVWEAHALLRAKPIAGDADLGQRFLDLVAPLRWPAGGLDATQVREIRTLKA
                     RMEAERLPRGADRKTHFKLGHGGLSDVEWVVQLVQLQHAHAHPELRTTSTMAALSAAE
                     SLGLIDPDHATDLAASWRLASSMRNAGVLFRAKTVDAVPTDPRDADGIARILGLPGGS
                     GQELGERYRKLARRARAAHEAEFYDD"
     gene            complement(4569..>5591)
                     /locus_tag="PL014_10790"
     CDS             complement(4569..>5591)
                     /locus_tag="PL014_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOT57619.1"
                     /translation="DPRHVLQRALTKAADLGFTFYTHPEIEFFLLKQGWVPGTRPEPI
                     DQAGYFDHVPHGNSHDFRRAAIGMLEAMGISVEFSHHEGAPGQNEIDLRYADALSTAD
                     NIMTFRAVIKEVALEQGIFASFMPKPFAEHPGSGMHTHLSLFEGDTNAFHEPGAPYQL
                     SRTGRQFIAGLLHHGAELTAVTNQWVNSYKRLWGGGEAPAHLTWGHNNRSAMVRVPMY
                     KPSKGNSTRVEVRSIDSACNPYLAFAVLLAAGLKGIEEGYELPPETEDDVWSLTDAER
                     KAMGIKPLPASLSDAISVMEGSELIAETLGEHTFDFFLRNKRAEWADYRNQVSAFELD
                     RYLPRL"
BASE COUNT          750 a         1927 c         2155 g          759 t
ORIGIN      
        1 ggaaccgggt acgagttctc gttggtgttc agggccaccg gcacgtcgag ctgcggagcg
       61 ccgtaggcgg tgcgcccgcg caggtcggcg cgcagcatcg cctcgacggt ggtgcgggaa
      121 tccatggcgc agagtctagg tcgctgcgcc ctgtggcaac ttttccgccc gacgcccgag
      181 cggcgccgcg gcgggctcag gcgctcgggt cgagctcggg gtgccgcgcg agcacggcct
      241 caccgtgggc gggcaggtcc tcggcgcggg cgagcgtgac gacgtggccg cccacctcgc
      301 gcagcgcctc cgacgagtac gtgacgacgt ggatgccgcg caggaagctc tgcaccgaca
      361 gcccgctgct gtggcaggca cacccacccg tgggcagcac gtggttggag ccggcggcgt
      421 agtcaccgag gctgaccggc gaccaggggc cgacgaagac cgcaccggca ttgcgcaccc
      481 gggcggcgac ggatgccgcg tcgcgggtct ggatctccag gtgctcggcc gcgtaggcgt
      541 tgaccagggc caggccggcc tcgacgtcgt cgacgaggac gatcgccgac tgccgaccgg
      601 ccagcgcggt ggccacccgc tcggtgtgct tggtcgtggc gacccgacgc gccagcgccg
      661 cctcgacggc atccacgagg tccggggaat cggtgaccag gacggctgca gcgagcgggt
      721 cgtgctcggc ctgggagatg aggtcggagg cgacgtggtc ggcatccgcg ctgtcgtcgg
      781 cgagcacggc gatctccgtg gggccggcct cggcgtcgat gccgatgatc cccttgagca
      841 ggcgcttggc cgcggcgacg tagatgttgc ccgggccggt gacgaggctg acggggtcgc
      901 agacctcacc gtcgtcctcg atgccgtacg cgaaggcagc cacggcctgc gccccaccca
      961 tggcccacac ctcgtccacc tccaggaggg cgcacgcggc gaggatcgtc gggtgcggcc
     1021 agccaccgtg gtcgcgctgc ggtgggctcg cgacggccag gctcggaacc tgcgcgagct
     1081 gggcgggcac gacgttcatg acgacgctgc tcgggtacac ggccaagcca ccgggcacgt
     1141 agagcccgac ccggtcgacg ggcacccagc gctcggtgac cgtgcccccg tcggcgacct
     1201 gcgtggtcgt gtcggaccgg cgctggtcgg cgtgcacgac gcgcgcccgg cggatggact
     1261 cctcgagggc cgcgcgcacg gccgggtcca gcgtcgtcag ggcgtcggcg atgacctcgg
     1321 cgggcacccg cagccgcgcc ggacggatgc cgtcgaagcg ctccgcggcg tcgtagaggg
     1381 ccgccgcacc gcgctcggcg acgtccgcga cgatcgggcg cacggcatcc aacgccgcct
     1441 cgacgtcgaa ctcggcgcgc ggcagggcag ccttgagctc acgcaggctg aggtgctgcc
     1501 cgcgcaggtc gagccgggga atgatcgtgt cgggcatgcc cccaagttta ggtcgagccc
     1561 tcagtcgtcg tagaactcgg cctcgtgggc ggcccgggcc cggcgagcca gcttgcggta
     1621 gcgctccccc agctcctggc ccgagccccc gggcaggccg aggatgcgcg cgatgccgtc
     1681 ggcatcgcgc gggtcggtcg gcacggcgtc caccgtcttg gcgcggaaga gcaccccggc
     1741 gttgcgcatc gacgacgcca ggcgccacga cgccgcgagg tccgtggcgt ggtcggggtc
     1801 gatcaacccg agcgactccg ccgccgacag ggccgccatc gtcgaggtcg tgcgcagctc
     1861 cgggtgggcg tgggcgtgct gcagctgcac gagctggacg acccactcga cgtcggacag
     1921 gccgccgtgg ccgagtttga agtgggtctt gcggtccgcg ccgcgcggca gccgctcggc
     1981 ctccatccgg gccttcaggg tgcggatctc gcgaacctgg gtcgcgtcga gcccgccagc
     2041 cggccagcgc agcggggcga ccaggtcgag gaagcgctgg ccgaggtcgg cgtcgccggc
     2101 gatcggcttc gcccgcagca gggcgtgcgc ctcccagacg agcgaccagc gcgcgtagta
     2161 ctcgcggtac gaggcgagcg agcgcacgag cgggccgttc ttgccctcgg ggcgcagccc
     2221 ggcgtcgatg tcgaggttgg ggtcgggccc gcgcaggccg agcaggcgga tgaggtcctt
     2281 gacgacctcg agggcctgcg cctgcgccgc cccctcgtcg gccccgtcga ccgggtcgtg
     2341 gacgaacagc acgtcggcgt ccgagccgta cccctgctcg ccgccaccga ggcgccccat
     2401 gccgacgacg acgaccctgg tgagggcagg ggcaccggtg cgggcctcga ggtcacgcac
     2461 gcacagctcg agggtcacct cgagcagcgc cgaggtgagg tcggtcatgg ccgtgccgag
     2521 cccgtcgagg gtgagcaggc cggacaggtc ggcgaccgcg acgcggaaga gctcggatcg
     2581 gcggatggtg cgggcggcga gcacggcgga tgccgggtcg tccttgcgcc ctgcggcgga
     2641 ccgcatccgg cgcaggacgt cctcgcgcgc acgcggccgt aggccggccg cgtcaccgag
     2701 gaactgcacg cactccggcg cctgctcgag cagaccggcg gcgaagcggc tgcgggccag
     2761 ggtgtgcgcg agccgctcgg cggccgagcc ctcgtcgcga agcagccgca ggtaccacgg
     2821 ggtggtcccg agctcgtcgc tgatcttgcg gaaggcgagc agacccgcat ccgggtctgc
     2881 ctcgtcggcg aaccagccga gcatcaccgg caacagctgg cgctggatcg cggcccgtcg
     2941 gctgagcccg tcggtcaagg cttcgaggtg gcggagcgcg ccatgcggat cgcggaaacc
     3001 caacgcggac agacgctctc gcgcggcctc gggagtcagg cgcacctcgg agtcggagag
     3061 gcgggccacg gccgagagca gcgggtggta gaagatccgc tcgtgcaggc ggcgcacctc
     3121 gcgctgctgg gccttccact gggcgatgac ggcatccgcc ggtgaggacc ggtgaccgag
     3181 cgcccgtccg aggcggcgca ggtccgaggc tgcggtgggc atgaggtggg tgcggcgcag
     3241 gcggtagagc tggatgcggt gttccagcgt gcgcagcagc cggtatgccg tggcgagcgt
     3301 cgcggcgtcc tcacgcccca cgtaaccacc cgccgacagg gccgcgagcc cgtcgagcgt
     3361 ggtcccggtg cgcagcgact catcggcccg gccgtgcacg agctggagca gctgcacgga
     3421 gaactcgacg tcgcgcagcc cacccgcacc gagcttgagc tggcgggcgg cctcggcggg
     3481 tgggatgtgc tgttcgacgc gtcggcgcat cgcctgcacg tcggcgacga agttctcgcg
     3541 ggtggaggct tgccagacca tcggctgcac ggcgtcgcag taggcctgcc ccacctcggc
     3601 gtcacccgcg accgggcggg ccttgagcag ggcctggaac tcccacgtct tggcccaccg
     3661 ctcgtagtag gagcggtggc tcgcgacggt gcgcacgagt ggcccgttct tgccctcggg
     3721 gcgcagggcc gggtcgacct gccacaacgc ccccgtggcc gtcgatgccg agcagatcct
     3781 catcaggcgc gtggcaaggt ccgtcgcgat cgccgtcgac tcctcctcgg gcaccccgtc
     3841 cgccggttcg gccacgaaga tgacgtcgac gtcggagatg tagttcagct cgcgcccacc
     3901 ggtcttgccc atggcgatga ccgccaggcg ggtgcgcccg gcatccgccc cgacctcgtc
     3961 gcgggcgatc acgagagcgg cctccagcgc cgcctgcgcc aggtcggcca gggccgcggc
     4021 cgtctccggg agggacacga gtgggtcgcc ggacgtgagg tccagggccg cgatctcgag
     4081 cagttgttcg cggtatgccg tgcgcaggac gtcgacggcc ggcgcaccct cgggggcgct
     4141 caccgccccg acgagccgct ggatgcgctg ctgcgcggtg aggggctgcg cccgcgtgac
     4201 ggcgctccac tggtgcgggt gggcgcacag gtggtcgccg agggccgccg agctgcccag
     4261 caccgcgagc agccgctgac gcgcggggcc cggtgccgcc agcgcctcgg tgaccgggcc
     4321 gcgaagctca gggtcgcggc cgacgacctc catgaagcgc accagcccca gcagggcgcc
     4381 gtcagggtcc gggacaccgg cgagggcagc ggcgagcccg tggtcctcga tgtgctcgcg
     4441 ggagtggatg agcccggcga gcgccgggtc ctgcaacagg ccgaacgccc gggcagcgtc
     4501 gtcgaacccc agccgcgcga tggcgacggg gatcgacccg ggaccgcgag agctcacccc
     4561 ggttccggtc agaggcgggg caggtagcgg tcgagctcga aggcgctcac ctggttgcgg
     4621 tagtcggccc actcggcccg cttgttgcgc aggaagaagt cgaaggtgtg ctccccgagg
     4681 gtctcggcga tgagctcgct gccctccatg acgctgatcg cgtcgctgag gctcgcgggc
     4741 agcggcttga tgcccatggc cttgcgctcg gcgtcggtga ggctccacac gtcgtcctcg
     4801 gtctcgggag gcagttcgta gccctcctcg atgcccttga gcccggcggc gagcagcacc
     4861 gcgaacgcca ggtaggggtt gcaggcgctg tcgatgctgc gcacctcgac ccgggtgctg
     4921 ttgcccttgc tcggcttgta catgggcacg cgcaccatgg cggagcggtt gttgtggccc
     4981 caggtgaggt gagccggtgc ctctcccccg ccccagaggc gcttgtagga gttgacccac
     5041 tggttggtga cggcggtgag ctcagcaccg tggtggagca ggccggcgat gaactgccgt
     5101 cccgtgcgcg agagctggta gggcgcgccg ggctcgtgga aggcgttggt gtccccctcg
     5161 aacagggaca ggtgggtgtg catgcccgac cccgggtgct cggcgaaggg cttgggcatg
     5221 aacgaggcga agatgccctg ctcgagggcg acctccttga tgaccgcccg gaaggtcatg
     5281 atgttgtcgg ccgtggagag tgcgtcggcg tagcgcaggt cgatctcgtt ctggccgggg
     5341 gcgccctcgt ggtggctgaa ctcgaccgag atgcccatcg cctcgagcat gccgatggcg
     5401 gcgcgccgga agtcgtggct gttgccgtgc ggcacgtggt cgaagtagcc ggcctggtcg
     5461 atcggctccg gcctggtgcc gggcacccag ccctgcttga gcaggaagaa ctcgatctcc
     5521 gggtgggtgt agaaggtgaa cccgaggtcg gcggccttgg tcagcgcccg ctggaggacg
     5581 tgccgggggt c
//
LOCUS       DAOSOC010000162         5559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_14753_length_5559_cov_10.859375, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000162 DAOSOC010000000
VERSION     DAOSOC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14753_length_5559_cov_10.859375"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PL014_10795"
     CDS             complement(<1..689)
                     /locus_tag="PL014_10795"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HOT57620.1"
                     /translation="MGDRLAGKVAVVTGGCSGIGLATVRRFAQEGAKVVIADLNDELG
                     EQYAAEVGGTYVHTNVADKADVDNLFKVAHDTYGRIDIAFNNAGISPPDDDSILDTEL
                     DAWDKVQLVNLTSVYLCCKAVLPYMLEQKSGSIINTASFVAVMGAATSQISYSASKGG
                     VLSMTRELGVQFAREGIRVNALCPGPVNTPLLKELFAKDAERAARRLVHVPMGRFGEP
                     EEMANAVLFLAS"
     gene            complement(699..2108)
                     /locus_tag="PL014_10800"
     CDS             complement(699..2108)
                     /locus_tag="PL014_10800"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT57621.1"
                     /translation="MTTTVTTDAVRHDVINPATEQVVTTIDLVGVEETDAAVARAVEV
                     GPAWRAVSPADRAQLLRRFADAVEAATDDLAALEVANSGHTIGNARWEVGNVVNVLRY
                     YSAAPERLFGRQIPVAGGLDVTFKEPLGVVSVIVPWNFPMPIAGWGLAPALAAGNTVV
                     LKPAELTPLTAIRLGELALDAGIPEGVFTVLPGKGSVVGERFVTHPDVRKVCFTGSTP
                     VGQGIMRKAADQLKRVTLELGGKSANIVFADADLDAAAASAPMSFLDNAGQDCCARTR
                     ILVQRSVFDAFMERFEQAMSQVVVGDPGDEATHMGPLISAGQRETVRGFVEGAADAGD
                     APVDVAFRGAAPEGAGWWYPPTVVLPRSTQDRVWREEVFGPVVSVMAFDDEADAIAKA
                     NDTAYGLSGSIWTRDVGRAIRVARGVEAGNLSVNSHSSVRYSTPFGGFKASGIGRELG
                     PDALDAFTEVKNVFINTEA"
     gene            complement(2120..3502)
                     /locus_tag="PL014_10805"
     CDS             complement(2120..3502)
                     /locus_tag="PL014_10805"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOT57622.1"
                     /translation="MSSPHSSGPPRLTIDQLRADILAGDVDTVIVAFTDMQGRLQGKR
                     LHGHFFLDHVLDHGTEGCNYLLAVDVDMNTVDGYRMSSWERGYGDMFFAMDLDTLRRT
                     PGHPNSATIQSDLTWLDGTGEVAQSPRTILKQQIARAADAGYGAFAGTELEFILFEDS
                     YDAAWDRRYHGLTGANRYNVDYSILGGSKVEPVLREIRNEMYRAGMTVESAKGECNFG
                     QHEIGFLYDEVLRTCDNHTIYKTGAKEIAARHGQALTFMAKYDEREGNSCHIHLSLRG
                     TDGDIVFADDDRDGGRSELFDSFLAGVLATMREFSYFYAPNINSYKRFAAGSFAPTAV
                     AWGTDNRTCSLRVVGHGAGLRVENRVPGGDVNPYLAVAAMLAGGLHGIREGLALEPAC
                     VGNAYVGDYEHVPSTLAQARDLLAGSAIAREAFGDEVVDHYVNAADVELAAFNSAVTD
                     WERVRGFERL"
     gene            complement(3520..4266)
                     /locus_tag="PL014_10810"
     CDS             complement(3520..4266)
                     /locus_tag="PL014_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOT57623.1"
                     /translation="MTPEQPSPPPTRLPDAVLRPAVGNVFETTVEQLATAIRLGVFVV
                     GEQLPPERDLAERLAISRNTLREAIAALRDAGLVETRRGRGGGTIVTDAGHAPGRAGE
                     PSAVNGIRRGAALEDALDFRRVVEPGAARLAATRTLSGDQRAWLLESCRAVRQADPAA
                     HRIADSRLHLAIATVTGSPMVVEAVTRAQAALGELLGAIPVLRTNIEHSHDQHDTIVA
                     AILTGDPDTARTTMEEHCDATSALLRGLIG"
     gene            complement(4325..5182)
                     /locus_tag="PL014_10815"
     CDS             complement(4325..5182)
                     /locus_tag="PL014_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOT57624.1"
                     /translation="MDDPPRVTWPSVLPRVVATDLDGTLLRSDGSISLRTRQVLADVE
                     RAGTSVLFVTARPPRWVHPLADAVGSHGVVIAGNGAFVVDLARGEVISTGCFTAADLL
                     PLVTDLRAAFPLAGLALERRTGMAVEASFLSPHPGDDDAVTVVEALADVPDLDEDPTG
                     KLLVVAPDGAGGPDFIAAVETVVGARGHVAYSGVANLAEISAPGTTKAAGLARWCSAR
                     GIDAAEVWAFGDMPNDLPMLRWAGTSFAVESGHPDVLAATTHTCPDNDRDGVATVLGH
                     ALAGGRTRR"
     gene            complement(5161..>5559)
                     /locus_tag="PL014_10820"
     CDS             complement(5161..>5559)
                     /locus_tag="PL014_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT57625.1"
                     /translation="QTYIEELGGMNLFFVTRDNHLVTPELTGSILEGITRGSILELAT
                     SLGLTPEERRIPIQEWKDGVASGEISEVFACGTAAVITPVGELRWEGGSAPSTAGEGG
                     GEVTKAIRTALLDIQYGRVEDTHGWMTRLA"
BASE COUNT          780 a         1874 c         2062 g          843 t
ORIGIN      
        1 gaggcgagga agagcacggc gttggccatc tcctcgggct cgccgaagcg gcccatgggc
       61 acgtggacga ggcggcgcgc ggcccgctcg gcgtccttgg cgaacagctc cttgagcagc
      121 ggggtgttga ccgggccggg gcacagcgcg ttgacccgga tgccctcgcg ggcgaactgc
      181 accccgagct cgcgggtcat cgacagcacc ccacccttgg acgccgagta cgagatctgc
      241 gaggtcgccg cgcccatcac ggccacgaag ctcgccgtgt tgatgatcga gccggacttc
      301 tgctcgagca tgtacggcag caccgccttg cagcacaggt acaccgacgt gaggttgacg
      361 agctggacct tgtcccaggc gtcgagctcg gtgtcgagga tcgagtcgtc gtcgggcggg
      421 ctgatgccgg cgttgttgaa ggcgatgtcg atgcgcccgt aggtgtcgtg agccaccttg
      481 aacaggttgt cgacgtcggc cttgtcggcg acgttggtgt gcacgtacgt gccaccgacc
      541 tcggccgcgt actgctcccc cagctcgtcg ttgaggtcgg cgatgacgac ctttgcgccc
      601 tcctgcgcga accgtcgaac ggtcgcgagg ccgatgccgg agcaccctcc ggtcacgacg
      661 gcgaccttgc cagcaagccg atctcccatg gtgctctcct atgcctcggt gttgatgaag
      721 acgttcttga cctcggtgaa cgcgtcgagc gcgtcggggc cgagctcgcg gccgatgccg
      781 ctggccttga agccgccgaa gggcgtcgag tagcgcaccg agctgtggct gttgaccgac
      841 aggttgccgg cctcgacgcc gcgggccacc cggatcgccc gaccgacgtc gcgggtccag
      901 atcgacccgg acagtccgta tgccgtgtcg ttggccttgg cgatcgcgtc ggcctcgtcg
      961 tcgaacgcca tgacggacac gaccggcccg aagacctcct cgcgccacac ccggtcctgc
     1021 gtgctgcggg gcagcacgac cgtgggcggg taccaccagc cggcgccctc gggcgccgcg
     1081 ccacggaacg cgacgtcgac cggcgcgtca ccggcatccg cagcgccctc gacgaagccg
     1141 cgaacggtct cgcgctgacc cgcggagatc agcgggccca tgtgcgtggc ctcgtcgccg
     1201 gggtcgccga cgacgacctg gctcatggcc tgctcgaagc gctccatgaa cgcgtcgaag
     1261 accgagcgct gcacgaggat ccgcgtgcgc gcacagcagt cctggccggc gttgtcgagg
     1321 aagctcatcg gggcgctggc cgccgcggcg tcgaggtcgg catcggcgaa gacgatgttg
     1381 gccgacttgc cgccgagctc gagggtgacc cgcttgagct ggtcggcggc cttgcgcatg
     1441 atgccctgac cgaccggcgt ggagccggtg aagcagacct tgcgcacgtc ggggtgggtg
     1501 acgaaccgct cgccgacgac cgatcccttt cctggcagca cggtgaacac gccctcgggg
     1561 atgccggcgt ccagcgcgag ctcaccgagc cggatcgccg tcaggggggt gagctcggcc
     1621 ggtttgagca cgacggtgtt gccggccgcg agcgccgggg ccaggcccca gcccgcgatc
     1681 ggcatcggga agttccacgg cacgatgacc gagacgaccc cgagcggctc cttgaaggtc
     1741 acgtcgagcc caccggcgac ggggatctgc cggccgaaga ggcgctcggg tgccgcggag
     1801 tagtagcgca gcacgttgac cacgttgccg acctcccacc gggcgttgcc gatggtgtgg
     1861 ccggagttcg ccacctcgag ggcggcgagg tcgtcggtgg cggcctcgac ggcgtcggcg
     1921 aaccggcgca gcagctgggc acggtcggcg gggctgacgg cgcgccaggc cggtccgacc
     1981 tcgacggccc gcgcgacggc cgcgtcggtc tcctcgacgc cgacgaggtc gatcgtggtg
     2041 acgacctgct cggtggcggg gttgatgacg tcgtgccgca cggcatccgt tgtcacagtg
     2101 gtggtcatgg tgtggggggt cacagcctct cgaagccgcg gacgcgttcc cagtcggtca
     2161 ccgcggagtt gaaggccgcg agttcgacgt ccgcggcgtt gacgtagtgg tcgacgacct
     2221 cgtcgccgaa ggcctcgcgg gcgatggcgc tgcccgcgag caggtcacgt gcctgagcca
     2281 gggtcgaggg cacgtgctcg tagtcgccga cgtaggcgtt gccgacgcac gccggctcca
     2341 gggccagtcc ctcgcggatg ccgtgcagcc cacccgcgag catcgccgcc accgcgaggt
     2401 aggggttgac gtcgcctccg gggacgcggt tctccacgcg cagcccggca ccgtggccga
     2461 cgacgcgcag gctgcacgtt cggttgtcgg tcccccacgc cacggcggtc ggggcgaagg
     2521 acccggcggc gaagcgcttg taggagttga tgttcggtgc gtagaagtag gagaactcgc
     2581 gcatcgtcgc gagcaccccg gcgaggaagc tgtcgaacag ctcgctgcgg ccgccgtcac
     2641 ggtcgtcgtc ggcgaagacg atgtccccgt cggtgccgcg cagtgacagg tggatgtggc
     2701 acgagttgcc ctcacgctcg tcgtacttgg ccatgaaggt cagcgcctga ccgtggcggg
     2761 cggcgatctc cttggccccg gtcttgtaga tcgtgtggtt gtcgcacgtg cgcagcacct
     2821 cgtcgtagag gaaaccgatc tcgtgctggc cgaagttgca ctcccccttg gccgactcca
     2881 cggtcatgcc ggcccggtac atctcgttgc ggatctcgcg cagcaccggc tcgaccttgc
     2941 tgccgccgag gatggagtag tcgacgttgt agcggttggc gcccgtcagg ccgtggtagc
     3001 gacgatccca cgcggcgtcg tagctgtcct cgaagaggat gaactcgagc tcggtgccgg
     3061 cgaaggcgcc gtacccggca tcggcggccc gggcgatctg ctgcttgagg atggtgcgcg
     3121 gggactgggc gacctcgccg gtgccgtcga gccacgtgag gtcgctctgg atggtggccg
     3181 agttcgggtg gcccggggtg cggcgcaggg tgtcgaggtc catcgcgaag aacatgtcgc
     3241 cgtaaccacg ctcccacgac gacatcctgt aaccgtcgac ggtgttcatg tcgacgtcga
     3301 cggcgagcag gtagttgcag ccctcggtgc cgtggtcgag cacgtggtcg aggaagaagt
     3361 gcccgtgcag gcgcttgccc tgaaggcgtc cctgcatgtc ggtgaaggcg acgatgaccg
     3421 tgtcgacgtc cccggcgagg atgtcggctc gcaactggtc gatcgtgagc ctgggcgggc
     3481 ctgatgagtg tggtgacgac atgcttaccc cttctggcgc tatccgatga gtccacgcag
     3541 gagcgccgac gtcgcgtcgc agtgctcctc catggtcgtg cgggcagtgt cggggtcacc
     3601 cgtgaggatg gccgcgacga tcgtgtcgtg ctggtcgtgc gagtgctcga tgttggtgcg
     3661 cagcaccggg atcgccccca gcagctcacc cagggccgcc tgggcccggg tcaccgcctc
     3721 gacgaccatc ggtgatccgg tgacggtggc gatcgccagg tgcaggcgcg agtcggcgat
     3781 ccggtgcgcc gccgggtcgg cctgtcgcac cgcccggcac gactccagca accaggcgcg
     3841 ctggtcaccg gacagggtgc gcgtggcggc gaggcgggcc gcgccgggtt cgacgacgcg
     3901 ccggaagtcc agggcgtcct cgagcgcggc accgcgtcgg atgccgttca ccgcggacgg
     3961 ttcgccggcc cgcccgggtg cgtggccggc atccgtgacg atcgtgccgc caccgcgtcc
     4021 gcggcgggtc tcgacgagac cggcgtcgcg cagcgcggcg atcgcctcgc gcagggtgtt
     4081 gcgggagatc gcgagccttt cggcgaggtc acgctcgggt gggagctgtt caccgacgac
     4141 gaagacgccg agccggatcg cggtggcgag ctgctcgacg gtggtctcga agacgttgcc
     4201 gacggccggg cgcaggacgg cgtcgggcag ccgggtgggc ggcggcgacg gctgctccgg
     4261 ggtcacggga gcgaggatag cggcgcaatg gtccttgtgt gaacctttga ccgcgactcg
     4321 gcggtcagcg acgggtgcga ccccccgcga gggcgtggcc gagcaccgtg gccacaccgt
     4381 cgcggtcgtt gtccgggcac gtgtgggtcg tcgcggccag gacgtccggg tgcccgctct
     4441 cgaccgcgaa ggaggtcccc gcccaccgca gcatcggcag gtcgttgggc atgtcaccga
     4501 aggcccagac ctcggccgcg tcgatgccgc gggccgaaca ccaccgggcc agcccggctg
     4561 ccttggtcgt gcccggcgcc gagatctcgg ccaggttggc gacccccgag taggcgacgt
     4621 ggccgcgcgc gccgacgacg gtctcgaccg cggcgatgaa gtccgggccg cccgcaccgt
     4681 ccggtgccac gacgagcagc ttgccggtgg ggtcctcgtc gaggtcgggg acgtcggcga
     4741 gcgcctcgac gacggtgacc gcgtcgtcgt cgcccgggtg cggggagagg aagctcgcct
     4801 cgaccgccat gccggtgcgc cgctccaggg ccagcccggc cagggggaag gctgcccgca
     4861 ggtcggtgac gagcgggagg aggtcggccg ccgtgaagca gccggtggag atcacctcgc
     4921 cgcgggcgag gtcgacgacg aacgcgccgt tgccggcgat gacgaccccg tgcgacccga
     4981 ctgcatcggc cagggggtgg acccaccggg gcggccgggc cgtcacgaac agcaccgacg
     5041 tccccgcccg ctcgacgtcc gccaggacct gcctcgtgcg cagggagatg ctgccgtcac
     5101 tgcgcagcaa cgttccgtcc aggtcggtgg cgacgacccg tggcaggacg ctcggccagg
     5161 tcacgcgagg cgggtcatcc atccgtgggt gtcctcgacg cggccgtact ggatgtccag
     5221 caacgccgtg cggatggcct tggtgacctc accgccaccc tccccggcgg tcgacggggc
     5281 cgaaccaccc tcccagcgca gctcgccgac gggcgtgatg actgcggctg tcccgcacgc
     5341 gaacacctcg ctgatctcgc cgctggcgac gccgtccttc cactcctgga tggggatgcg
     5401 gcgttcctcc ggcgtcaggc ccagcgacgt cgcgagctcg aggatggagc cgcgggtgat
     5461 gccctcgagg atcgacccgg tgagctcggg ggtcacgagg tggttgtcgc gggtgacgaa
     5521 gaacaggttc atccccccga gctcctcgat gtaggtctg
//
LOCUS       DAOSOC010000163         5430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_15167_length_5430_cov_8.862698, whole genome shotgun sequence.
ACCESSION   DAOSOC010000163 DAOSOC010000000
VERSION     DAOSOC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15167_length_5430_cov_8.862698"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /gene="efp"
                     /locus_tag="PL014_10825"
     CDS             <1..494
                     /gene="efp"
                     /locus_tag="PL014_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT57626.1"
                     /translation="EFQHVKPGKGPAFVRTKLKNVMSGKVVDKTFNAGVKVETANVDK
                     RNMQYLYNDGTDFIFMDGDTYDQIAVSPEVVGDAAKYMLENQDAIVARHDGTVLFIEL
                     PASVVLEISYTEPGLQGDRSTGGTKPATLETGAEIAVPLFLETGTKVKVDTRDGSYLG
                     RVS"
     gene            498..911
                     /gene="nusB"
                     /locus_tag="PL014_10830"
     CDS             498..911
                     /gene="nusB"
                     /locus_tag="PL014_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT57627.1"
                     /translation="MAARTKARKRALDLLFEAEQRQVNVGELLRQRLAAPVTEAPLSE
                     FTGDLVSGVVEHWTQIDELIATYSQGWTLARMPAVDRSILRLGAYEVLYSTDVPEAVA
                     ISEWVGLATELSTDDSPKFVNGLLGRLVEVKPTLA"
     gene            complement(1050..1274)
                     /locus_tag="PL014_10835"
     CDS             complement(1050..1274)
                     /locus_tag="PL014_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57628.1"
                     /translation="MQTSVQPLTPRDASCADTSRASGALDGGDVGPAAVVAPMRAQLF
                     PPRDADAETRRRWLASSPWPEVVFASAGAT"
     gene            1508..2098
                     /gene="pyrR"
                     /locus_tag="PL014_10840"
     CDS             1508..2098
                     /gene="pyrR"
                     /locus_tag="PL014_10840"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOT57629.1"
                     /translation="MTRADSIVPPGQPLDPDGRTVLTASDVARALRRIAHEILEHNKG
                     PDDLVVLGIPSRGVELANRLVDLIREVEGAEVPVGSLDITMHRDDLRHQPTRAPMHTQ
                     IPSGGIDDKVVVLVDDVLYSGRTIRAALDALSDLGRPRAVRLAVLVDRGHRELPIRAD
                     HVGKNLPTSHAEKVQVRLAGHDGLEDVVRIAGGDAR"
     gene            2095..3042
                     /locus_tag="PL014_10845"
     CDS             2095..3042
                     /locus_tag="PL014_10845"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOT57630.1"
                     /translation="MNRHLLSSADLTRDDVRMLLATASEMHEVQRRDVKKLPTLRGRT
                     IINLFFEDSTRTRSSFEIAGKWMSADTINITGKGSSASKGESLRDTVRTIDAMAVDAI
                     VMRHMASGAAHQVAQWVDASVINAGDGTHEHPTQALLDAYTIEQRLGDLDGAHVAIVG
                     DLTHSRVFRSNAIMLRTLGARVTLVAPPTLMPSGIRPWAAADGLTLSDDLDAVLASGP
                     DAVMMLRVQRERMSGGFFPTPREYTVGFGLTRDRLRALADANSDAVICHPGPMNRGLE
                     ISADAADAARSLVLDQVSAGVAVRMSVLYHLLAGEGVAA"
     gene            3039..4343
                     /locus_tag="PL014_10850"
     CDS             3039..4343
                     /locus_tag="PL014_10850"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOT57631.1"
                     /translation="MSDLLVIGADVLGGGAADVLVRDGLVAAVGADATADARSGAERL
                     DADGLVLLPGFVDLHTHLREPGREDAETIASGSRAAAVGGFTAVLAMANTNPVTDTAE
                     AAERIRDLGAAAGLVDVVPVGAVTKGLDGAELAELGLMHRSRARVRVFSDDGRCVADG
                     RVMRRALEYVRAFGGVISQHSQDPSLAGPQACCHEGETSGRLGLPGWPAVAEASIIAR
                     DAMLAEHTGSRVHVAHVSTAEGLDVVRWAKARGIAMTAEVTPHHLALGTDLLAGYDPV
                     YKVNPPLRPDTDRAALIEGLLDGTIDAIATDHAPHARHDKEHAFVDAAFGMLGLETAF
                     AVVHDLLVADGRLPWSDLARLMSVAPAAIAGLADHGRPITPGEPANLVLVDPTASVTV
                     DRDSSQSLSRNTPWHGASLTGAVRTTILRGRVTAHEGAPTSC"
     gene            4337..5299
                     /gene="carA"
                     /locus_tag="PL014_10855"
                     /pseudo
     CDS             4337..5299
                     /gene="carA"
                     /locus_tag="PL014_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851343.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
BASE COUNT          846 a         2074 c         1782 g          728 t
ORIGIN      
        1 tcgagttcca gcacgtcaag cccggcaagg gcccggcctt cgtgcgcacc aagctgaaga
       61 acgtcatgag cggcaaggtc gtcgacaaga ccttcaacgc cggtgtcaag gtcgagaccg
      121 cgaacgtcga caagcggaac atgcagtacc tctacaacga cggcaccgac ttcatcttca
      181 tggacggtga cacctacgac cagatcgcgg tcagccccga ggtcgtcggt gacgccgcca
      241 agtacatgct cgagaaccag gacgcgatcg tggcccgcca cgacggcacc gtgctgttca
      301 tcgagctccc cgcgtcggtc gtcctcgaga tctcgtacac cgagccgggc ctccagggcg
      361 accgctctac cggcggcacc aagccggcga cgctcgagac cggcgccgag atcgccgttc
      421 cgctgttcct ggagaccggc accaaggtca aggtcgacac ccgcgacggc tcctacctcg
      481 gtcgggtcag ctgacccgtg gccgcccgca ccaaggcccg caagcgcgcg ctcgacctgc
      541 tcttcgaggc cgagcagcgc caggtcaacg tcggtgagct gctgcggcag cggctggccg
      601 ctcccgtgac cgaagccccg ctgtcggagt tcaccggtga cctcgtcagc ggggtcgtcg
      661 agcactggac ccagatcgac gaactgatcg cgacgtactc acagggctgg accctggcca
      721 ggatgccggc cgtcgaccgc tcgatcctgc gcctgggcgc ctacgaggtg ctctactcca
      781 cggatgtccc cgaggccgtc gcgatcagcg agtgggtggg gctggccacc gagctgtcca
      841 ccgatgacag cccgaagttc gtcaacggcc tcctgggccg cctggtcgag gtcaagccga
      901 ccctggcctg agccgcattc gaaccccagg tgcacggatt cgcgagcggt acacctcgac
      961 ccgcggtcac tcctcgaggc ggaagccgat cttgagcccc acctggtagt gggcgatcgc
     1021 accgtcgaag gcgcggacgg ggttgcggct caggtggcgc cggcggacgc gaagacgacc
     1081 tccggccacg gtgagctggc cagccagcgc cgacgggtct cggcgtcggc gtcgcgcggg
     1141 gggaacagct gcgctcgcat gggagccacg accgccgccg ggccgacgtc gccgccgtcg
     1201 agggcacctg acgcccgtga ggtatcggcg cacgacgcgt cacgcggggt gaggggctgg
     1261 acggacgtct gcacgatggc ctccttctcg ggtgggggag gccccgctct ggcgcctcac
     1321 acgagccagt agatcacgaa atgtttggtc aggcatagca tcgcgttatg tgagtccatc
     1381 atccggacct ggtgtccggc acgcgaacct cgtcaggggc tcggccgcgg accccgtaca
     1441 gtggttcgcg caccaacaga catcctttaa cgacctgtcc cgtgaggcag ggaaggaggt
     1501 ccgcgtcatg acgcgcgccg acagcatcgt gccccccgga caacccctcg accccgacgg
     1561 ccgtaccgtc ctcactgcga gtgacgtcgc ccgggccctg cgccgtatcg cccacgagat
     1621 cctcgagcac aacaaggggc ctgatgatct cgtcgtcctc ggcatcccca gtcgaggcgt
     1681 cgagctggcc aaccggctcg tcgacctcat ccgcgaggtc gagggcgctg aggttcccgt
     1741 gggcagcctg gacatcacca tgcaccgcga cgacctgcgc caccagccca ctcgggcccc
     1801 gatgcacacg cagatcccga gcggcggcat cgacgacaag gtcgtcgtcc tcgtcgacga
     1861 cgtgctctac tccgggcgca ccatccgcgc agccctcgac gcgctctccg atctgggccg
     1921 tcctcgcgcg gtgcgactgg ccgtgctcgt cgaccgcggc caccgcgagc tgccgatccg
     1981 ggccgaccac gtcggcaaga acctgccgac cagccacgcc gagaaggtcc aggtgcgcct
     2041 ggccgggcac gacggcctcg aggacgtcgt gcggatcgcc gggggtgacg cccgatgaac
     2101 cgccacctgc tctccagcgc cgacctgacc cgcgacgacg tgcggatgct gctcgcgacg
     2161 gcatccgaga tgcacgaggt gcagcgccgc gacgtcaaga agctgcccac gctgcgtggg
     2221 cgcacgatca tcaacctctt cttcgaggac tccacccgca cccgcagcag cttcgagatc
     2281 gcgggcaagt ggatgagcgc cgacaccatc aacatcaccg gcaagggcag cagcgccagc
     2341 aagggtgaga gcctgcgcga caccgtgcgc accatcgacg ccatggccgt cgacgccatc
     2401 gtcatgcgcc acatggccag cggagccgcc caccaggtgg cgcagtgggt cgacgccagc
     2461 gtcatcaacg ccggcgacgg cacccacgag cacccgaccc aggccctgct cgacgcctac
     2521 accatcgaac agcggctcgg agacctcgac ggtgcccacg tcgcgatcgt cggcgacctc
     2581 acccacagcc gcgtgttccg ctccaacgcg atcatgctgc ggaccctggg cgcccgggtc
     2641 accctcgtcg cccctccgac gctgatgccg agcggcatcc gcccgtgggc tgccgccgac
     2701 ggcctcaccc tctccgacga cctcgacgcc gtgctcgcga gcgggccgga cgccgtcatg
     2761 atgctgcgcg tgcagcgcga gcggatgagc ggcggcttct tccccacgcc acgcgagtac
     2821 acggtcggct tcgggctgac ccgcgaccgc ctgcgagccc tggccgacgc caactccgac
     2881 gccgtcatct gccaccccgg cccgatgaac cgcggcctgg agatcagcgc cgacgccgcc
     2941 gacgccgccc gcagcctcgt gctcgaccag gtctccgcag gggtggcggt gcggatgtcc
     3001 gtcctctacc acctgctcgc cggagagggg gtggcggcgt gagtgacctg ctggtcatcg
     3061 gtgccgacgt cctcggcgga ggagccgccg acgtgctcgt ccgcgacggc ctcgtcgccg
     3121 cggtcggtgc ggacgccaca gccgacgcgc gctccggggc agagcggctc gacgccgacg
     3181 ggctggtcct gctgcccggc ttcgtcgacc tgcacaccca cctgcgcgaa cccggccgcg
     3241 aggacgccga gacgatcgcc tccggttcgc gcgccgcggc cgtcggcggg ttcaccgccg
     3301 tgctcgccat ggccaacacc aacccggtca ccgacaccgc cgaggccgcc gagcggatcc
     3361 gcgacctcgg cgccgccgcg gggctcgtcg acgtcgtgcc ggtcggggcc gtcaccaagg
     3421 gtctcgacgg ggccgagctc gccgagctcg gcctcatgca ccgctcccgg gcacgagttc
     3481 gggtgttctc cgacgacggc cgctgcgtcg ccgacggccg ggtcatgcgc cgggccctgg
     3541 agtacgtgcg cgcgttcggg ggtgtcatct cccagcacag ccaggacccg agcctggctg
     3601 ggccgcaggc ctgctgccac gagggcgaga cctcgggacg gctcggcctg cccggctggc
     3661 cggcggtggc cgaggcgagc atcatcgccc gcgacgcgat gctcgccgag cacaccgggt
     3721 cgcgggtgca cgtcgcccac gtcagcacgg cagagggcct cgacgtcgtg cgctgggcca
     3781 aggcccgggg catcgcgatg accgccgagg tgaccccgca ccacctggcg ttgggcaccg
     3841 acctgctggc cggctacgac ccggtctaca aggtcaaccc gccgctgcgc cccgacaccg
     3901 accgggcggc cctgatcgag ggcctgctcg acggcaccat cgacgcgatc gccaccgatc
     3961 acgccccgca cgcgcggcac gacaaggaac acgcgttcgt cgacgccgcg ttcggcatgc
     4021 tcgggctcga gaccgccttc gcggtcgtcc acgacctgct cgtcgccgac ggccgcctgc
     4081 cgtggagcga cctcgcccgg ctgatgtcgg tcgccccggc cgccatcgcc gggctggccg
     4141 accacggccg cccgatcact cccggcgagc ccgccaacct cgtgctcgtc gaccccacag
     4201 cctcggtcac cgtcgaccgg gactcctcgc agtccctgtc ccgcaacacc ccgtggcacg
     4261 gcgccagcct caccggcgcc gtccgcacga cgatcttgcg cggtcgcgtc accgcgcacg
     4321 aaggagcgcc cacctcatgc tgaccacccg cgagcccgcc gtcctcgtgc tcgaggacgg
     4381 ccgcacgttc accggccggg cctacggcca tcgcggcagc acggtcggtg aggccgtctt
     4441 caacaccggc atgaccggtt accaggagac cctcaccgac cccagctacc accgccaggt
     4501 cgtcgtcatg acggcccccc acgtcggcaa caccggctgg aacgacgagg acgacgagtc
     4561 cggtcgcatc tgggtctccg gcttcgtcgt gcgcgaccct gccctgcggc cctcgaactg
     4621 gcgctcccag cgctccctcg aggacgagct cgacgcgaag ggtgtcgtcg gcatctgcga
     4681 cctcgacacc cgggcgctga cccggcacct gcgcgagcgc ggcgccatgc gcgccggcat
     4741 cttctccggg gatgccgcga gcgcacctcg cgagcgactc ctcacccagg tcatcgacgc
     4801 accgtcgatg accggcgcgg cgctcgccga gcaggtggcg acccaggtgg cctacgtcgt
     4861 gccggcggtg ggggagaggc gcttcaccgt ggccgcggtc gacctcggca tcaaggcgat
     4921 gacgccgacg atgatggcgc agcgcggcat cgaggtgcac gtgctgcccg ccaccacgac
     4981 catcgaggag atccgggcga tcgcccctga cggggtgttc ttctccaacg gccccggcga
     5041 cccgagcacc gccgagcgcc aggccgacct gctgcgcgcc gtgctgcgcg acggcatccc
     5101 ctacttcggc atctgcttcg gcaaccagat cctcggcaag gcgctcgggt tcggcaccta
     5161 caagctgaag tacggccacc gcggcatcaa ccagccggtc atggaccgca cgaccggcaa
     5221 ggtcgaggtg accgcgcaca accacgggtt cgccgtcgac gcgccgctcg acacggtcac
     5281 cgagaccgag ttcggcagcg tcgcggtcag cgaggacttg atctccttga acagcaccga
     5341 cgcgagccct tccaggacct tcagcgcgac gttcccggtg aacccgtcgg tcaccacgac
     5401 gtccactttc cctgcaggga tgtcgcgacc
//
LOCUS       DAOSOC010000164         5426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_15184_length_5426_cov_8.441631, whole genome shotgun sequence.
ACCESSION   DAOSOC010000164 DAOSOC010000000
VERSION     DAOSOC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15184_length_5426_cov_8.441631"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PL014_10860"
     CDS             complement(<1..1018)
                     /locus_tag="PL014_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT57632.1"
                     /translation="MTRPVLRQVHNIGRRRAWVIWLVALSVYILAVFHRTSLGVAGLL
                     AADRFGISAAQLATFTVLQLAVYAAMQIPVGVLIDRYGSKRLMVCGLTLMTVGQMWFA
                     FAGTFEAGIAARVLLGAGDAMIFTSLLRLVAVWFRVKQAPFITQLTGMAGQLGAIAAA
                     TPLAAALATWGWTRSFATAAGIGIVLGVLLLAVVKDSPYRGESVERIKVRALARTLAE
                     VWGNPGTQLGLWVHFTSQFGATVFVMLWGFPFLVSGEGVDEDVASWLLIVMTVTGMAA
                     GPAVAAFTARIPYRRSQLVLGIVGTIMAVWAVVLLWPGPAPLWLLVVLVVVTALGGPG
                     SMVGF"
     gene            complement(1045..1518)
                     /locus_tag="PL014_10865"
     CDS             complement(1045..1518)
                     /locus_tag="PL014_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57633.1"
                     /translation="MNLRLAAALVTALVATGGCAAPTQESPDATTSPNPASTTPAAST
                     TPSPTASESPSPGTGSTLPADLRTRPEVAAAIADTAQRAQVDPEKVLVAAWSPVTWND
                     GSLGCPQKGMDYTQALVDGELLMLRVEQALFQYHSARGGPFTYCANPDASYTVTG"
     gene            complement(1532..2692)
                     /locus_tag="PL014_10870"
     CDS             complement(1532..2692)
                     /locus_tag="PL014_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT57634.1"
                     /translation="MAATRLMPTEEGQDLIDLTREICDKELRPKVDDAERAAATDESF
                     PTEVFRTLGAAGLLSLPHPEEFGGYGQPYEVYLQVVEEIASAWMSVAVGVSVHSLTAY
                     PVTTFGTPEQQSTLLPDMLSGEQLGAYCLSEQQAGSDIGSMTTRATTDETSGEYVLRG
                     RKAWISHAGHADYYTTFARTSDDGGKGLSCFIVPGDATGLSFGAPERKMGLHCDTVRE
                     VMYDDVRVDGARRIGAEGQGMAIALSALDAGRLGIAAAATGLAQSALDLAASYAKERQ
                     QFGRAIGANQGLAFLIADMEAAVTSARATYLHAARLKDAGRPFSKEAAVAKLTATDAA
                     MRVTTDAVQVLGGAGYTQDFPAERFMREAKVTQIFEGTNQIQRLVISRHVLR"
     gene            complement(2812..3903)
                     /locus_tag="PL014_10875"
     CDS             complement(2812..3903)
                     /locus_tag="PL014_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57635.1"
                     /translation="MTGGAGGLDVDGLIERGQFSLRLTASVAPGEVVAVLGPNGAGKS
                     TLLRALAGLVELKDGSIRLDGVVLDDAANDTWVPPELRPVGIVFQDYRLFPHLDVLDN
                     IAFAPRSMGASRAASRATAREWVGRLGLTELAHHRPGQLSGGQAQRVALARSLAADPR
                     LLLLDEPLAALDARTRLDVRTALRAHLEQFAGPVVVVTHDPIDAMVIADRIIVIEQGR
                     VVQEGTPAEVARHPATDYVARLVGLNLYAGTRDPASAVVNLDGGGTLTVSPTPGTDAD
                     ARRLLVALPPGAVSLFTTPVENTSARNVWTGAVTGIELLGDRVRVEVTGSPSCLVDIT
                     PAALADLGLERGTPVWLTAKATETIAYPA"
     gene            complement(3900..4718)
                     /locus_tag="PL014_10880"
     CDS             complement(3900..4718)
                     /locus_tag="PL014_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT57636.1"
                     /translation="MKRSARQPIRRETAPWPLAVPAAVGVTFLLVPLAAMLLRTPWSS
                     LPDILRDPAVLDALRLSLVTATSSTAVAVVLGVPLAWVLARTHFRGLSLLRALVTLPL
                     VLPPVVGGVALLLAFGRLGVVGRWLDEWFGFTLPFTTLGVVLAQCFVAMPFLVVTVEG
                     AFRGLDRGFEEAASTLGASRLATFRRVTLPLIAPSLAAGTVLCWARALGEFGATITFA
                     GNSPGITQTMPLAVYLAMESDPEAAIALSLVLLTVSVAVLALLRDRWLRPGSTL"
     gene            complement(4715..>5426)
                     /gene="modA"
                     /locus_tag="PL014_10885"
     CDS             complement(4715..>5426)
                     /gene="modA"
                     /locus_tag="PL014_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT57637.1"
                     /translation="PGSSSTQSASSSLSGTVTVFAAASLQESFTTFEEQFEQANPDVD
                     VILNLGASSALATGITQGQPGDVFASASQSTMDTVIAAGAAETATAFARNTMQIAVPP
                     ANPAGITALSDLTRSGVKVALCQAQVPCGKAAATVFDNASLTVVPVTQESDVKAVLTK
                     VRLGEVDAGIVYVTDVRAAGGEVTGIDIPADVNASTTYPITTLTESENPAAARAFVEH
                     VLSEEGAEVLASAGFATP"
BASE COUNT          812 a         1806 c         2095 g          713 t
ORIGIN      
        1 cgaagccgac catcgacccg gggccgccga gggcggtcac gacgacgagc acgaccagca
       61 gccacaacgg cgccggcccg ggccacagca gcacgacggc ccagacggcc atgatcgtgc
      121 cgacgatgcc gagcaccagc tgggagcggc ggtaggggat gcgtgcggtg aaggccgcca
      181 cggccgggcc ggcggccatg ccggtgacgg tcatgacgat gagcagccac gacgcgacgt
      241 cctcgtcgac cccctcaccg ctgaccagga acgggaaccc ccacagcatg acgaacacgg
      301 tggcgccgaa ctgcgaggtg aagtgcaccc acagcccgag ctgggtaccg gggttgcccc
      361 acacctcggc cagcgtccgg gccagggcgc ggaccttgat ccgctcgacc gactcgcccc
      421 ggtacggcga gtccttgacg acggcgagga gcagcacgcc gaggacgatg ccgatgccgg
      481 ccgcggtggc gaacgagcgg gtccagcccc aggtggccag ggccgcggcg aggggggtcg
      541 ctgcggcgat ggctccgagc tggccagcca tgccggtcag ctgggtgatg aacggggcct
      601 gcttgacccg gaaccacacg gcgaccaggc gcagcagcga ggtgaagatc atcgcgtcgc
      661 ccgcgccgag caggacgcgg gcggcgatcc cggcctcgaa ggtccccgcg aaggcgaacc
      721 acatctggcc gacggtcatc agggtgagcc cgcagaccat gaggcgcttg gagccgtacc
      781 ggtcgatgag caccccgacg gggatctgca tcgccgcgta gaccgcgagc tggagcacgg
      841 tgaacgtcgc gagctgggcg gccgagatgc cgaagcggtc ggccgcgagc agccccgcga
      901 cgccgagcga ggtgcggtgg aagacggcga ggatgtagac cgagagcgcg accagccaga
      961 tcacccaggc acggcgacgg ccgatgttgt gcacctggcg cagcacgggt cgggtcacca
     1021 cccgagtgta ggagccgggt ggggtcagcc ggtgaccgtg tagctggcgt ccgggttggc
     1081 gcagtacgtg aagggcccgc ctcgggcgga gtggtactgg aacagggcct gctcgacgcg
     1141 cagcatgagc agttcaccgt cgacgagcgc ctgcgtgtag tccatgccct tctgcgggca
     1201 gcccagcgaa ccgtcgttcc acgtgaccgg ggaccacgcc gcgacgagta ccttctcggg
     1261 gtcgacctgg gcgcgttggg cggtgtcggc gatggccgcc gcgacctcgg ggcgggtgcg
     1321 gaggtcggcg ggcagggtgc tgccggtgcc gggagacgga ctctcggatg ccgtcgggct
     1381 gggtgtcgtg gatgccgcgg gcgtcgtgct cgcggggttt ggtgaggtcg tggcgtcggg
     1441 gctttcctgc gtcggtgcgg cgcacccgcc cgtcgccacg agggcggtga cgagtgcggc
     1501 agccagtcgc aggttcatgg cgggcacgtg atcagcgcag gacgtggcgg ctgatgacga
     1561 ggcgctggat ctggttggtg ccctcgaaga tctgggtgac cttggcctcg cgcatgaatc
     1621 gctcggcggg gaagtcctgg gtgtacccgg ccccgccgag aacctggacg gcatccgtcg
     1681 tgacccgcat cgcggcatcc gtggcagtga gcttggcgac cgcggcctcc ttggagaagg
     1741 ggcggccggc gtccttgagg cgggcggcgt gcaggtaggt ggcgcgggcg ctggtcaccg
     1801 cggcctccat gtcggcgatg aggaaggcca gcccctggtt ggcaccgatg gcgcggccga
     1861 actgctggcg ctccttcgcg tacgacgcgg cgaggtcgag ggccgactgg gccagaccgg
     1921 tggcggcggc agcgattccc aggcggccgg catccagcgc ggacagggcg atggccatcc
     1981 cctgcccttc ggcgccgatg cggcgggcgc cgtcgacgcg cacgtcgtcg tacatcactt
     2041 cgcgcacggt gtcgcagtgc aggcccatct tgcgctcggg ggcgccgaag gacaggccgg
     2101 tggcgtcacc ggggacgatg aagcaggaca ggcccttgcc gccgtcatcg gaggtgcggg
     2161 cgaaggtcgt gtagtagtcg gcgtgcccgg cgtggctgat ccacgccttg cgaccgcgca
     2221 gcacgtactc gcccgaggtc tcgtcggtcg tcgcgcgggt ggtcatcgag ccgatgtcgc
     2281 tgccggcctg ctgctcggac aggcagtagg cgccgagctg ctcgcccgag agcatgtcgg
     2341 gcagcagggt gctctgctgc tcgggggtgc cgaacgtcgt gaccgggtat gcggtcagtg
     2401 agtgcaccga gacgccgacg gccacgctca tccacgcgga ggcgatctcc tcgacgacct
     2461 ggaggtagac ctcgtagggc tgcccgtagc cgccgaactc ctcggggtgg gggagggaga
     2521 gcaggcccgc ggcgcccagc gtgcggaaga cctcggtggg gaaggactcg tcggtggcgg
     2581 cggcccgttc ggcgtcgtcg accttgggcc gcagttcctt gtcgcagatc tcgcgggtca
     2641 ggtcgatgag gtcctgaccc tcctcggtgg gcatcagccg cgtcgctgcc atggcccgac
     2701 catacccact cccggctttt cctccccatg agggaagcac aaggttcgca agggatgcct
     2761 tcccttgcgg acctgacctt ttccgcccca ggcaggcacg gtcgagcggc gtcaggccgg
     2821 gtaggcgatg gtctcggtcg ccttggccgt cagccacacc ggggtgccgc gttcgaggcc
     2881 gaggtcggcc agcgccgcgg gggtgatgtc gaccaggcag gacggagacc cggtcacctc
     2941 gacgcgcacg cggtcaccga ggagttcgat gccggtcacc gcgccggtcc acacgttgcg
     3001 cgcactggtg ttctcgacgg gagtggtgaa caggctgacc gctcccggag ggagcgccac
     3061 gagcaggcgc cgcgcgtcgg cgtcggtgcc cggggtcggc gacaccgtga gggttccgcc
     3121 gccgtcgagg ttcaccaccg cggatgccgg gtcacgcgtc cccgcgtaga ggttgagtcc
     3181 gacgaggcgg gcgacgtagt ccgtcgcggg atggcgggcg acctccgcgg gggtgccttc
     3241 ttggacgacc cgcccttgct cgatgacgat gatccggtcg gcgatgacca tggcgtcgat
     3301 cgggtcgtgg gtgacgacga ccacagggcc ggcgaactgc tcgaggtggg cacgcaacgc
     3361 cgtgcggacg tcgagccgcg tccgggcatc gagggccgcg agcggttcgt cgagcaggag
     3421 caggcgggga tcggcggcca gggaccgcgc cagggcgacc cgctgggcct gccctcccga
     3481 gagctgcccc ggccggtggt gggcgagttc ggtgaggcca agccggccca cccactcccg
     3541 ggcagtggcg cgcgaagccg cccggctcgc gcccatggag cggggggcga aggcgatgtt
     3601 gtcgaggacg tcgaggtgcg ggaacagccg gtagtcctgg aacacgatgc cgaccggtcg
     3661 cagctccggc gggacccacg tgtcgttggc ggcgtcgtcg agcaccaccc cgtcgagccg
     3721 gatcgagccg tccttgagct cgacgagacc cgcgagcgcg cgcagcaggg tcgacttgcc
     3781 ggcgccgttg gggcccagga cggccacgac ctcacccggc gccacggatg ccgtgaggcg
     3841 cagggagaac tggccgcgct cgatgaggcc gtcgacgtcg aggcctccgg cgcccccggt
     3901 cacagcgtgc tcccgggccg cagccagcgg tcgcgcagca gcgcgagcac cgccaccgag
     3961 acggtgagca gcacgagcga cagggcgatc gccgcctccg gatcgctctc catcgcgagg
     4021 tagaccgcga gcggcatcgt ctgggtgatg ccgggggagt tgccggcgaa ggtgatggtg
     4081 gcgccgaact cgcccagggc tcgtgcccag cacagcacgg tgcccgcggc cagcgacggc
     4141 gcgatcaggg gcagcgtcac cctccggaag gtggccagcc gcgacgcccc caaggtggag
     4201 gccgcctcct cgaagccgcg gtccagaccc cggaaggcgc cctcgacggt gacgacgagg
     4261 aacggcatcg ccacgaagca ctgcgcgagg accaccccca gcgtcgtgaa gggcagggtg
     4321 aacccgaacc actcgtcgag ccagcggccc accaccccga ggcgcccgaa cgcgagcagc
     4381 agcgcgacgc ctccgacgac cgggggcagc acgagcggga gggtcaccag ggcccgcagc
     4441 agcgacaggc cacggaagtg ggttcgggcg aggacccagg ccagcggcac cccgagcacg
     4501 acggccacgg ctgtcgaact ggtcgcggtg accagggaca gtcgcagggc atcgagcacc
     4561 gcggggtcgc gcaggatgtc gggcaggctg ctccagggcg tgcgcagcag catggccgcg
     4621 agggggacga ggaggaaggt gacgccgacg gcagcgggga cggcgagtgg ccacggcgcc
     4681 gtctcgcggc gaatgggctg ccgcgccgag cgtttcacgg ggtggcgaac cctgctgacg
     4741 cgagcacctc ggctccctcc tcggagagga cgtgctcgac gaaggcgcgg gcagcggcgg
     4801 ggttctccga ctccgtcagc gtggtgatgg ggtaggtcgt gctggcgttg acgtcggccg
     4861 ggatgtcgat gcccgtgacc tcgccgccgg cggcccggac gtcggtgacg tagacgatgc
     4921 cggcgtccac ctcgcccagc ctgaccttgg tgagcaccgc cttgacgtcc gactcctgcg
     4981 tcacgggcac caccgtgagg gaggcgttgt cgaacaccgt cgcggcggcc ttgccgcacg
     5041 gcacctgggc ctggcacagg gccaccttga cgcccgacct cgtgaggtcg gacaaggcgg
     5101 tgatccccgc cgggttggcc ggtgggaccg cgatctgcat cgtgttcctc gcgaaggccg
     5161 tcgccgtctc cgcggctccg gcggcgatga cggtgtccat cgtcgactgg ctcgcggagg
     5221 cgaacacgtc gcccggctgt ccctgcgtga tgccggtggc cagggccgag ctcgccccga
     5281 ggttcaggat gacgtcgacg tcggggttcg cctgttcgaa ctgctcctcg aacgtcgtga
     5341 acgactcttg gagcgacgcg gccgcgaaca ccgtgacggt gccggagagg ctgctcgagg
     5401 ccgactgcgt gctggacgat ccgggg
//
LOCUS       DAOSOC010000165         5389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_15305_length_5389_cov_8.080240, whole genome shotgun sequence.
ACCESSION   DAOSOC010000165 DAOSOC010000000
VERSION     DAOSOC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15305_length_5389_cov_8.080240"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..616
                     /locus_tag="PL014_10890"
     CDS             98..616
                     /locus_tag="PL014_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57638.1"
                     /translation="MPTDSGWTPSVPEAVHVTRGNQESTAAGIYGVIVSSAVMSASHA
                     DSAGKVIGAVLITLMVYWGAERYARIVAERIHEGHRPNWSTVRHQLTHGWEIVTASTL
                     PLLVLAVFTVLGSELFTAVLAALICSTALLCVAGWAVGRHGRLSTGERIVSAIVAGMF
                     GVFFIALKTLLH"
     gene            691..2385
                     /locus_tag="PL014_10895"
     CDS             691..2385
                     /locus_tag="PL014_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT57639.1"
                     /translation="MGHLDVNSVCFALPDGRELLRDVTFRVGEGAKVALVGPNGTGKT
                     TLLRILAGDLTASDGAVTRSGGLGVMRQFIGKVRDDTTVRDLLVSVAPEAVRLAAAEI
                     DRTELMMMERDSETDQLAYAQALADWGDVGGYEWETHWDVCTVAAIGTPFERAQWRKV
                     STLSGGEQKRLVLESLLRGPEEVLVLDEPDNYLDVPAKRWLEERLIESPKTVLFVSHD
                     RELLDRVATRIVTLEPSHGGNTVWVHPGRFSTYDQARSDRNDRLDELLRRWDEEHAKI
                     KALVLMYRQKAAFNDGLASRLQAAETRLRKFEDAGPPEAVPLHQKVTMRLTGGRTAKR
                     AVVATGLELRVDGGQLMKPFDAEIWFGERIGVLGSNGSGKSHFLRLLAAGGSDPDREH
                     EPVGDIRPAPVEHAGRVVLGSRVRPGWFAQTHDHASLHGRTLLEILHRGDEHRAGMPR
                     EVAARALDRYGLAKASEQTFETLSGGQQARLQILLLELSGATLLLLDEPTDNLDLHSA
                     EALEDALAAFEGTVLAVTHDRWFARGLDRFLVFRSDGRVVASDEPVWDEGRVGRAR"
     assembly_gap    2539..2638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2934..>5389
                     /locus_tag="PL014_10900"
     CDS             2934..>5389
                     /locus_tag="PL014_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT57640.1"
                     /translation="MPAPRRLTAVLIGGAVALTPALLTAVPAEAATASVALVGDLQSE
                     VGCPDDWDPACTQSELSRVGDTDIYKGTFEVPEGDFKFKVALNDGWDENYGAGGTFNG
                     DNIPLLLAGPASITFTFDDATNMVTFAPADLDEGTTAADAKLAGNSLRSALTRERFYF
                     VMADRFANGSTANDRGGLTGGPLKTGFDPTHKGFYHGGDLKGITEKLDYIKGMGTTAI
                     WLTPSFKNKPVQGGPGQESAGYHGYWITDFTQIDPHLGTNDDMKALIKAAHRKGMKVF
                     FDIITNHTADVIDYEEKQYTYRSKKAYPYEDAAGEPFDDREYVSKPASQWPELDAGSF
                     PYTPFLAEGDEDAKTPAWLNDPTMYHNRGDSTFAGESSTYGDFIGLDDLFTERPEVVD
                     GMGEIYKSWVEFGIDGFRIDTVKHVNLEFWQKFVPDILGAADRIGNDDFFAFGEVYDG
                     NPAVMSEYTTAGKLQATLDFGFQQQGVDFAKGNPTDVLSDFFAKDDWYTDPDSNAYQL
                     PTFLGNHDMGRASMFLARDGQSEAEHLARVKLAQSLMFTTRGNPVTYYGDEQGFVSTG
                     GDQAAREDMFASQVDLYNTETVLGGPEGSRDRFNTKHPLYNHVKTLSQVRAAHPALAD
                     GAQIARYATDGPGIFAISRIGSDTKGKPGNAAGESKGKGEGKGKGKGKGKDAGRVEYV
                     VAANNGTKAATASFPTWTKGKGAVFTPVFGTDTAVKPAADGTVKVTVPPLGISVWRAN
                     TPISTSGAAPTVEVVLPESGVNGRAEIGADVKASTFAQTTFLWRQLGAKEWQPLGTDD
                     NAPFRVFHDTSGLPLSTPLE"
BASE COUNT          884 a         1870 c         1773 g          762 t
ORIGIN      
        1 gaggtggtgt accccgcgga tgccgacctc gccagccgcg ccgacatggc acgatcacgt
       61 cgagaactgc ccgcggaaca gctcgaagga gtccccgatg cccactgact ccggctggac
      121 tccctcggtg cccgaggcgg tgcacgtcac ccggggcaac caggagtcga ccgcggccgg
      181 catctacgga gtcatcgtca gctcggccgt catgtcggcc tcgcacgcgg actccgccgg
      241 caaggtcatc ggggccgtgc tcatcacgct catggtctat tggggcgctg agcggtacgc
      301 gcgcatcgtg gccgagcgca tccacgaggg gcaccgcccc aactggtcga cggtgcgcca
      361 ccagctgacc cacggctggg agatcgtcac ggcgtccacc ctgccgctgc tcgtcctggc
      421 cgtgttcacg gtgctcgggt cggagctgtt caccgcagtg ctcgccgccc tgatctgcag
      481 caccgccctg ctctgcgtcg ccgggtgggc cgtcgggcgc cacggtcggc tgtcgacggg
      541 ggaacgcatc gtctcggcga tcgtggccgg catgttcggt gtcttcttca tcgcactgaa
      601 gacgttgttg cactgaccct gccggtcccc atccgcgtgc tggcgaaact cgggtgaggt
      661 cgcggcctcg gtcgtggaca ctggaccctc gtgggccatc tcgatgtcaa ctccgtgtgc
      721 ttcgccctcc ccgacgggcg agaactgctg cgtgacgtca ccttccgggt gggggagggg
      781 gccaaggtcg ccctggtcgg cccgaacggc accgggaaga ccaccttgct gcgcatcctc
      841 gccggcgacc tcacggcatc cgacggggcc gtgacgcgca gcggtggtct cggggtcatg
      901 cggcagttca tcggcaaggt gcgcgacgac accacggtgc gtgacctgct cgtgtcggtg
      961 gctcctgagg cggttcgcct cgcggccgcc gagatcgacc gcaccgagct gatgatgatg
     1021 gaacgggact ccgagaccga ccagctcgcg tacgcgcagg cgctcgccga ctggggtgat
     1081 gtcggtggct acgagtggga gacccactgg gacgtgtgca ccgtggcggc catcggcacc
     1141 ccgttcgagc gagcccagtg gcgcaaggtc tccaccctgt ccgggggcga gcagaagcgg
     1201 ctcgtgctcg agtcgttgct gcggggtccc gaggaggtgc tcgtgctcga cgagccggac
     1261 aactacctcg acgtcccggc caagcgctgg ctcgaggaac gcctcatcga gtcgccgaag
     1321 accgtgttgt tcgtcagcca cgaccgtgag ctgctcgacc gggtggcgac gcggatcgtg
     1381 accctggagc ccagccacgg cggcaacacc gtgtgggtcc accccgggcg gttctcgacc
     1441 tacgaccagg cccggtccga ccgcaacgac cgcctcgacg agctgctgcg ccgctgggac
     1501 gaggagcacg cgaagatcaa ggccctcgtg ctcatgtacc ggcagaaggc cgcgttcaac
     1561 gacgggctgg cctcacggct gcaggccgcc gagaccaggc tgcgcaagtt cgaggatgcc
     1621 gggccgcccg aggcggtgcc gctgcaccag aaggtcacca tgcgcctcac cggtggccgc
     1681 accgccaagc gtgcggtggt ggcgaccggg ctcgagctgc gggtcgacgg cgggcagctg
     1741 atgaagccct tcgatgccga gatctggttc ggcgaacgca tcggtgtgct ggggagcaac
     1801 ggctcgggga agtcgcactt cctccgcctc ctcgcggccg gaggcagcga ccccgaccgc
     1861 gagcacgagc cggtgggtga catccgccct gcgccggtcg agcacgccgg acgggtggtg
     1921 ctcggttccc gggtgcgccc cggctggttc gcccagacgc acgaccacgc cagcctgcac
     1981 ggccgcaccc tgctcgagat cctccaccgt ggggacgagc accgagccgg gatgccgcgg
     2041 gaggtcgccg cgcgcgccct cgaccgctac ggactcgcca aggcctcgga gcagaccttc
     2101 gagacgttgt ccggcgggca gcaggcgcgg ttgcagatcc tgctgctcga gctctcgggg
     2161 gcgaccctgc tgctgctcga cgagccgacc gacaacctcg acctgcactc ggccgaggcc
     2221 ctcgaggacg cgctcgccgc gttcgagggc accgtcctgg cggtgaccca tgaccggtgg
     2281 ttcgcacgcg ggctcgaccg gttcctcgtg ttccggtccg acggtcgcgt ggtcgccagc
     2341 gacgagccgg tgtgggacga ggggcgggtg ggccgggccc gctgaggggc ggacctcaca
     2401 ccgctgcgcc cttgttgcct cgtcagggca gcgggagact gcaggtatgg acgcgcagct
     2461 caacaaggcc cgcacgctcg acgcggtcaa cgccctcaac cgcggagacc tcgacgggta
     2521 cctcgcgatc tacgccccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     2641 gctcggcctg atctgagcgc tgccgtcgcg gtcggggcac tgctggccga cgggccccac
     2701 cgggataccg cggcacgctt cggcgcccgg gtccggtcct ccgcaggagc cgtgggtgcg
     2761 gctgatgcgc tcgaggccgc cctgccgtcc catgcgaaca ccaagtgatg gtcaaggaat
     2821 cgtaagttct gtgcgccctc ccttcatgta aagcgtttac gcaactagtg tccagcgcgg
     2881 atcaatgccg tccgcgcgcc catcccgtcg cgcggtcctg aggaggagca ccgatgcccg
     2941 caccccgacg cctgaccgcc gtcctgatcg gtggcgcggt cgcgctcacc ccagcgctcc
     3001 tgacggccgt acccgcagag gcggccacgg catccgtcgc cctggtgggc gacctgcaga
     3061 gcgaggtcgg gtgtccggac gactgggacc ctgcgtgcac ccagagcgag ctgtcccgtg
     3121 tcggcgacac cgacatctac aagggcacct tcgaggttcc ggagggtgac ttcaagttca
     3181 aggtggccct caacgacgga tgggacgaga actacggtgc gggtggcacc ttcaacggcg
     3241 acaacatccc gctgctgctc gccggcccgg cgtcgatcac cttcaccttc gacgacgcga
     3301 cgaacatggt caccttcgca cccgccgacc tcgacgaggg aaccaccgcg gctgacgcga
     3361 agctggccgg gaactcgttg cgctcggcgc tcacccgtga gcggttctac ttcgtcatgg
     3421 ctgaccggtt cgccaacggg tccaccgcca acgaccgcgg tgggctcacg ggtgggccgc
     3481 tgaagaccgg cttcgacccc acccacaagg gcttctacca cggtggtgac ctcaagggca
     3541 tcaccgagaa gctcgactac atcaagggca tgggcaccac cgcgatctgg ctgacaccca
     3601 gcttcaagaa caagccggtt cagggcggcc ccggccagga gagcgccggc taccacgggt
     3661 actggatcac cgacttcacc cagatcgacc cgcacctcgg caccaacgac gacatgaagg
     3721 cgctcatcaa ggccgctcac cgcaagggca tgaaggtctt cttcgacatc atcaccaacc
     3781 acaccgcaga cgtcatcgac tacgaggaga agcagtacac ctaccgctcc aagaaggcgt
     3841 acccctacga ggacgccgcc ggtgagccgt tcgacgaccg cgagtacgtg agcaagcccg
     3901 cctcgcagtg gcccgagctc gacgccggat ccttcccgta caccccgttc ctcgccgagg
     3961 gggacgagga cgccaagacc ccggcctggc tcaacgaccc gacgatgtac cacaaccggg
     4021 gcgactcgac cttcgccggt gagtcctcga cgtacggcga cttcatcggc ctggacgacc
     4081 tgttcaccga gcggcccgag gtcgtcgacg gcatgggtga gatctacaag agctgggtcg
     4141 agttcggcat cgacggcttc cggatcgaca ccgtgaagca cgtcaacctc gagttctggc
     4201 agaagttcgt gccggacatc ctcggcgcgg ccgaccgcat cggcaacgac gacttcttcg
     4261 ccttcggcga ggtctacgac ggcaaccccg cggtgatgtc ggagtacacg acggccggca
     4321 agctccaggc cacgctcgac ttcgggttcc agcagcaggg cgtcgacttc gccaagggca
     4381 acccgaccga cgtgctgagc gacttcttcg ccaaggacga ctggtacacc gaccccgaca
     4441 gcaacgccta ccagctgccg accttcctcg ggaaccacga catgggccgg gcctcgatgt
     4501 tcctggcgcg cgacgggcag agcgaggccg agcacctggc acgcgtcaag ctcgcgcagt
     4561 cgctgatgtt cacgacccgc ggcaaccccg tgacctacta cggcgacgag cagggcttcg
     4621 tcagcacggg cggggaccag gccgctcgcg aggacatgtt cgccagccag gtagacctct
     4681 acaacaccga gacagtgctc ggcggccccg agggatcgag ggaccgcttc aacaccaagc
     4741 accctctcta caaccacgtg aagaccctct cccaggtgag ggccgcacac ccggccctcg
     4801 cagacggcgc ccagatcgct cgttacgcga ccgacggccc cggcatcttc gcgatctcgc
     4861 gcatcggctc cgacaccaag ggcaagccgg gcaacgccgc gggcgagagc aagggcaagg
     4921 gagagggcaa gggcaagggc aagggcaagg gcaaggatgc cggtcgcgtc gagtacgtcg
     4981 tcgcggccaa caacggcacc aaggccgcca cggcatcctt cccgacctgg accaagggca
     5041 agggcgcggt cttcacaccg gtgttcggca cggacacggc ggtcaagccg gcggccgacg
     5101 gcaccgtgaa ggtcaccgtg ccgccactgg gcatcagcgt gtggcgggcc aacaccccga
     5161 tcagcacctc cggggcggcg ccgacggtcg aggtcgtcct ccctgagtcg ggggtgaacg
     5221 ggcgcgccga gatcggcgcc gacgtgaagg cgagcacctt cgcgcagacg acgttcctgt
     5281 ggcgtcagct cggcgccaag gagtggcagc cgctcggcac cgacgacaac gcaccgttcc
     5341 gggtcttcca cgacacgtcc ggtctgccgc tgtccacgcc gctggagta
//
LOCUS       DAOSOC010000166         5386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_15316_length_5386_cov_10.205778, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000166 DAOSOC010000000
VERSION     DAOSOC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15316_length_5386_cov_10.205778"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1294
                     /locus_tag="PL014_10905"
     CDS             65..1294
                     /locus_tag="PL014_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT57641.1"
                     /translation="MDAVRTLENEVRELIRRSGLDPSRDEVAVRRLVGEAVSDYDERS
                     LSGGMPVLADLEAASRSVWDSVAGYGPLQQYFDDPSVEEIWVNEPSKVFVARNGVAEL
                     TTTLLTQDQVRDLVERMLKSSGRRVDLSTPFVDAVLPDGSRLHVVIPDITRDHWFVNV
                     RKFVVKADSMDDLVRLGTVTRQAAHFLEAAVVSGLNILVAGGTQAGKTTFLNCLGAAV
                     PPRERIVTCEEVFELRIPHRDVASMQCRQPSLEGTGEIPLRRLVKEALRMRPSRIVVG
                     EVRQAESLDLLIALNSGLPGMATVHANSAREAVTKMCTLPLLAGENVSARFVVPTVAS
                     SIDIVVHAALERDGRRVVREIAAVPGRVEGDVVEIADLFVRRRGELRRAEGFPPHEER
                     FERAGYDLSALLAPGVP"
     gene            1291..2160
                     /locus_tag="PL014_10910"
     CDS             1291..2160
                     /locus_tag="PL014_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT57642.1"
                     /translation="MTGLLLGLTFGVGIFLIWWSLWVPTPREPRSVTRVGPLERLSDE
                     IVQAGFTGLTVRTLIAACVIAFVLVLAFVQAAVGVLPIAGCFALMASFAPVALVRRRA
                     RMRRARLRDLWPDAVDNITSGVRAGMALPEALSQLGTRGPDELREPFLAFADDYRLSG
                     RFHDCLDRLKERLSDPVGDRLVESLRIAREVGGTDLGHLLRTLSGFLREDARTRAELE
                     TRQSWTVNAARLAVAAPWLVLAMLSTNPQSVRAYASGLGFVVLAVGGALTVLAYWSML
                     RIGRLPEDERVLR"
     gene            2157..3089
                     /locus_tag="PL014_10915"
     CDS             2157..3089
                     /locus_tag="PL014_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT57643.1"
                     /translation="MSGVARSGAILGFLMGCGLLLIWVGLPRHRRPGLAGRVVPYLRD
                     TPRPSRLLDIERAPKGAVAAVTAPIVKDLGALVERIMGGADSVRRRQLRAGVAADVDR
                     FRAEQVLWGALGAAVGGLLGTTALLVRDAGAVTVVMMTLIGAGVGITGADYNLSRRAQ
                     RREARMLMEFPTVAELLALAVAAGEGAVGALDRVCRLSSGELAGELRRCLADARAGAN
                     LPTALQGLADRTGLPSLRRFVDGVVIAVQRGTPLAEVLRAQAQDVREEGRRGLMEAGG
                     RKEIAMMVPVVFLILPVTVLFAVFPGFSYFQFTL"
     gene            3112..3321
                     /locus_tag="PL014_10920"
     CDS             3112..3321
                     /locus_tag="PL014_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57644.1"
                     /translation="MSITRTLSARLVVATHVVLTRAVRRSERGDVPGWVLITLMTAGL
                     VTALWVVAKGQLVSMFQNALSSVRG"
     gene            3331..3711
                     /locus_tag="PL014_10925"
     CDS             3331..3711
                     /locus_tag="PL014_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOT57645.1"
                     /translation="MPRHDERGAAVADFVMVAVLVVMLFLAVFQLGLTLHTRNTLISC
                     ASEGARLGARLDAVPGQGVARTQSLISQSLSPEYARKVSARESVVDGVRVVEVRVVAP
                     IPVLGLLGPNDRLDVVGRAFLEAQ"
     gene            3708..4169
                     /locus_tag="PL014_10930"
     CDS             3708..4169
                     /locus_tag="PL014_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOT57646.1"
                     /translation="MTDHGPRRSGQRVRDERGAAVVEFIALAVLMLIPLIYLVMMMAR
                     LQAGSFAVSQAAREAGRAFVTAESGSDAAGRAQSAARIAFLDHSFDGTGRLSIACDGT
                     PCLRPDGRVETTATVRVPLPLIPAFAREVIPMSVPVSATHLSTVDRFRGLP"
     gene            4190..4657
                     /locus_tag="PL014_10935"
     CDS             4190..4657
                     /locus_tag="PL014_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HOT57647.1"
                     /translation="MPAGPAPRRPRQEDGSISIFIVGLTVIAMILIAGTIAVTSAHLS
                     RMRLLDVADGAALTAANGLDDSAYTSGVKTSVPLSNATVRERAAGYVASRPRPNGILA
                     WRLGPGTGTPDGRTAVVSLSGEAELPMIGAVLSDLGVSIRITVQSSARADLEP"
     gene            4737..>5386
                     /locus_tag="PL014_10940"
     CDS             4737..>5386
                     /locus_tag="PL014_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOT57648.1"
                     /translation="MAVDFSQEIKDLRTTMASVREVTDLSKLEATIADFEQQASDPNL
                     WDDQEKAQVVTSGLSRAKAEHERVTNMDARIDDLQALVEMGQEENDADTLAEAEAELA
                     VVKKAVGELEVRTLLAGEYDERAAVVTIRAGAGGVDAADFAEMLMRMYLRWAERHGYP
                     VKVLDTSYAEEAGLKSATFEVDVPYAYGNLCVEGGTHRLVRISPFDNQGRRQTSFAA"
BASE COUNT          719 a         1865 c         1901 g          901 t
ORIGIN      
        1 tctcaggttc gatgtgagtc tgatggtcgt cgaggggcgg tcgcggggga gggagggctt
       61 ggtcatggat gccgtgcgca cgctcgagaa cgaggtgcgc gagctcatcc gtcggtcggg
      121 gttggatccc tcacgcgacg aggtggccgt gcgccggctc gtcggcgaag cggtgtcgga
      181 ctacgacgag cgttcgctct ccggtgggat gccggtgctc gccgatctgg aggccgcctc
      241 gcggtcggtg tgggacagcg tcgccggcta cgggccgctc cagcagtact tcgacgaccc
      301 gagcgtggag gagatctggg tcaacgagcc gtcgaaggtg ttcgtggcac gcaacggggt
      361 ggccgaactg acgacgaccc tgttgacgca agaccaggtc cgcgacctcg tcgaacgcat
      421 gctcaagtcg tcgggccggc gggttgacct ctcgacgccc ttcgtggatg ccgttctccc
      481 agacgggagt cggcttcacg tcgtcatccc cgacatcacc cgtgaccact ggttcgtcaa
      541 cgtccgcaag ttcgtggtca aggccgattc gatggacgac ctcgtgcggc tcggcacggt
      601 gacacgccag gcggcgcact tcctcgaggc ggccgtcgtt tccggtctca acatcctcgt
      661 cgcgggtggg acccaggccg gcaagacgac cttcctcaac tgtctggggg ccgcggtgcc
      721 gcctcgcgag cggatcgtca cctgtgagga ggtcttcgag ttgcggatcc cgcaccgtga
      781 cgtcgcgtcg atgcagtgtc gccagccctc gctcgagggc acgggtgaga tcccgctgcg
      841 ccgcctggtc aaggaggcgc tgcgcatgcg gccgtcgcgg atcgtcgtcg gcgaggtgcg
      901 ccaggccgag agcctggacc tgctcattgc gctcaactcc gggcttcccg gcatggcgac
      961 cgttcacgcc aacagcgccc gtgaggccgt cacgaagatg tgcaccctgc cactgctcgc
     1021 gggggagaac gtctcggccc ggttcgtcgt gccgaccgtc gcctcatcga tcgacatcgt
     1081 cgtccatgcg gccctcgagc gtgacggtcg acgcgtcgtg cgcgagattg ccgccgtgcc
     1141 cgggcgcgtc gagggcgacg tcgtcgagat cgccgacctc ttcgtcaggc gtcggggcga
     1201 gctccggcgc gctgagggct tcccgcccca tgaggagcgg ttcgaacgcg cgggatacga
     1261 cctctccgcg ctgttggcac cgggtgtgcc atgaccggct tgctgctcgg cctgaccttc
     1321 ggcgttggga tcttcctcat ctggtggagc ctctgggtgc cgaccccgcg agagcccagg
     1381 tcggtcaccc gcgtcggccc ccttgagcgg ctgtccgacg agatcgtcca ggcggggttc
     1441 accggactca cggttcgcac actgatcgcc gcgtgcgtca tcgccttcgt cctcgtgctg
     1501 gccttcgtgc aggcggccgt gggggtccta ccgatcgctg gctgcttcgc cctcatggcc
     1561 tcgtttgccc ccgttgccct ggtgcgtcga cgggcccgga tgcggcgggc tcgcctgcgc
     1621 gatctgtggc cggatgccgt cgacaacatc acgtcggggg tgcgggccgg gatggccctg
     1681 cccgaggcgt tgtcacagct gggcacgcgc ggcccggatg agctgcgcga gccctttctc
     1741 gcctttgccg atgactaccg cctgagcggg cggttccacg actgcctcga ccggctcaag
     1801 gagcgactca gcgacccggt cggtgaccgc ttggtcgaga gcctgcgcat tgctcgagag
     1861 gtgggcggca ctgatctcgg gcacctcctg cgcacgctgt cgggcttcct gcgtgaagac
     1921 gcgcgcacgc gggccgaact cgagacgcgg cagtcgtgga ccgtcaatgc cgcacgactg
     1981 gccgtcgccg cgccgtggct cgtgctcgcg atgctgtcca cgaacccgca gtcggtgcgc
     2041 gcgtacgcca gcgggttggg gttcgtggtg ctggccgtcg gcggcgcact cacggtgctc
     2101 gcgtactggt cgatgctgcg catcggtcgc ctgcccgagg acgagcgggt gctgcggtga
     2161 gcggtgtggc gcgctcgggg gcgatcctcg gcttcctcat gggctgtggc ctcctgctca
     2221 tctgggtagg gctgccccga caccgccggc ccgggctcgc gggacgagtg gtcccctacc
     2281 tgcgtgacac acccagaccg tcgagactgc tcgacatcga gcgggcgccc aagggggccg
     2341 ttgccgccgt caccgccccg atcgtcaagg acctcggcgc cctggtcgag cggatcatgg
     2401 gcggcgccga ctcggtgcga cgacggcagt tgcgcgccgg cgtggcggcc gacgtcgacc
     2461 ggtttcgtgc agagcaggtg ctgtggggcg ctctcggtgc tgcggtcgga ggcctcctcg
     2521 gtaccacggc cctcctcgtg agggatgccg gtgccgtgac cgtggtgatg atgacgctca
     2581 tcggtgccgg ggtcggcatc accggtgccg actacaacct ctcccgccgg gcccagcgcc
     2641 gcgaggcgcg catgctcatg gagttcccca ccgttgccga gctcctcgcc ctggcggttg
     2701 ccgccggtga gggcgcagtc ggtgccctcg accgggtctg ccgactgtcc agcggcgagc
     2761 tggccggcga gctgcgacgc tgcctggctg atgcccgcgc cggtgcgaac ctgcccaccg
     2821 cgctgcaagg gctcgcggac cgcaccgggc tgccgagcct gcgccggttc gtcgacggcg
     2881 tcgtcatcgc cgtccagcgc ggcacaccgt tggccgaggt gctgcgcgcg caggcgcagg
     2941 acgtccgtga ggaaggtcgg cggggcctga tggaggcagg cggtcgcaag gagatcgcga
     3001 tgatggtccc ggtcgtgttc ctcatcctcc ccgtcaccgt gctcttcgcc gtgttccccg
     3061 gcttcagcta cttccagttc acgctctgac ccgatggaaa ggaccgaccc catgtcgatc
     3121 acccgcaccc tgtccgcccg ccttgtcgtc gccacccatg tggtcctgac gcgcgcagtc
     3181 cgcagatcag agcgaggtga cgtgccgggc tgggtgctca tcaccctcat gaccgcgggc
     3241 ctggtcacgg cgctgtgggt cgtcgccaag ggacagctgg tctcgatgtt ccagaacgcc
     3301 ctctcctcgg tgaggggctg agctgtcgcg atgcctcggc acgacgagcg gggtgcagcc
     3361 gtggcggact tcgtcatggt ggccgtgctc gtcgtcatgc tcttcctcgc ggtgttccaa
     3421 ctcggtctga cgttgcacac ccgcaacacc ctcatctcgt gtgcgtccga gggggctcgc
     3481 ctgggtgcga ggctggatgc cgttcccgga cagggcgtcg cgcgtaccca gtcgctgatc
     3541 agccagtcac tgtcgccgga gtacgcacgt aaggtcagcg ctcgggagag cgtcgttgac
     3601 ggggtccggg tggttgaggt gagagtcgtc gcgcccatcc cggtgctcgg tctgcttggt
     3661 ccgaacgatc gtctcgacgt cgtcggtcgc gccttcctgg aggcgcagtg accgaccacg
     3721 gcccccggcg aagtggccag cgcgtccgcg acgagagggg tgccgcggtg gtggagttca
     3781 tcgccctcgc cgtgctgatg ctgatcccgc tcatctacct cgtgatgatg atggccagac
     3841 tccaggcggg ctcgttcgcc gtcagccagg ccgcccggga ggcgggccgg gccttcgtca
     3901 ccgcggagag cggcagcgac gctgcgggcc gggcccagtc cgcggccagg atcgccttcc
     3961 tcgaccactc cttcgacggc acggggaggt tgtcgatcgc gtgtgacgga accccgtgtc
     4021 tgcgacccga cgggcgggtg gagaccacgg caaccgtgcg ggtgccgctg ccgctgatcc
     4081 ccgccttcgc gcgcgaggtc atcccgatgt cggtcccggt gtcggcgacc cacctgtcga
     4141 ccgtggatcg tttccggggc ctgccgtgag cgggcgggat cgacctgtgg tgccggccgg
     4201 cccagctccg cgtcggccgc ggcaggaaga cggctcgatc agcatcttca tcgtgggcct
     4261 cacggtcatc gcgatgatcc tcatcgcggg gacgatcgcc gtcacctcgg ctcacctgtc
     4321 ccggatgcgg ttgctcgacg tcgctgacgg cgcagccctc accgcggcca acggcttgga
     4381 cgactccgcc tacacctcgg gggtgaagac ctcggtgccg ctgtcgaacg cgacggtccg
     4441 cgagcgcgcc gccggctacg tcgcgagccg cccaaggccc aacggcatcc tcgcgtggag
     4501 gctcggaccc ggcacgggca cgcccgacgg acgcacggca gtagtctcgc tcagcggtga
     4561 ggcagaactg ccgatgatag gggccgtgct gagcgacctc ggggtgagca tccgcatcac
     4621 cgtgcagtcc agcgcccgcg ccgacctcga accctgagac ctggaacgct tatggctgct
     4681 gtcgcagacc cgtgcccgca cggtcacggc cggggactcg cgtaggctgg ctcaccgtgg
     4741 ctgtcgactt ctcccaggag atcaaggacc tccgcacgac catggcgtcc gtgcgtgagg
     4801 tgaccgacct gtccaagctc gaggcgacca tcgccgactt cgagcagcag gcctcagacc
     4861 cgaacctgtg ggacgaccag gagaaggccc aggtcgtgac ctccgggctc tcgcgcgcca
     4921 aggccgagca cgagcgcgtc acgaacatgg acgcccgcat cgacgacctc caggccctgg
     4981 tcgagatggg ccaggaggag aacgacgccg acaccctggc cgaggccgag gccgagctgg
     5041 ccgtcgtgaa gaaggccgtc ggtgagctcg aggtgcgcac cctgctcgcc ggcgagtacg
     5101 acgagcgggc cgcggtcgtg accatccgcg ccggcgccgg gggagtggac gccgccgact
     5161 tcgccgagat gctcatgcgc atgtacctgc gctgggccga gcgccacggt taccccgtga
     5221 aggtcctcga cacctcctac gccgaggagg ccggcctcaa gagcgccacc ttcgaggtcg
     5281 acgtgcccta cgcctacggc aacctctgcg tcgagggcgg cacccaccgc ctcgtgcgga
     5341 tcagcccctt cgacaaccag ggccgccgcc agaccagctt cgccgc
//
LOCUS       DAOSOC010000167         5341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_15482_length_5341_cov_9.285282, whole genome shotgun sequence.
ACCESSION   DAOSOC010000167 DAOSOC010000000
VERSION     DAOSOC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15482_length_5341_cov_9.285282"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..751)
                     /locus_tag="PL014_10945"
     CDS             complement(2..751)
                     /locus_tag="PL014_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOT57649.1"
                     /translation="MVVLTSDTVTSGALPLALAVAVLAGLVSFASPCVLPLVPGFLGY
                     VTGLTGEPLAQRSRSRMVTGALLFVLGFTLVFVLGSIFVTSAGRALIEHRVLLMRLGG
                     IVVIVMGLVFLGVGGQREAKIAWRPKAGLAGAPLLGAVFALGWAPCTGPTLAAVLVMA
                     SATADPQVARSVTLATAYGLGLGLPFILIAAGLDRAGRASGWLRRHQRTIQVVGGVML
                     VLVGLLMVTGVWEDLNTWLKIELVDRFQVSL"
     gene            complement(793..1416)
                     /locus_tag="PL014_10950"
     CDS             complement(793..1416)
                     /locus_tag="PL014_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT57650.1"
                     /translation="MSGRPLVRWAGWVAAATLLLGTAACTSDPNSVAEQAKAGDQKGY
                     VSGDGAVETIPAADRAEPVRLSGQLLDGADWSSDSARGEVLVLNVWGSWCAPCVAEAP
                     DLQKVWSELEAQDAPVQFMGINFREDPARGAAFEKRAGITYPSLSDESGVAILALQGK
                     APTVPTTLVLDREGRIAARVNGPVTASTLRALVEDAVAESSPTASPS"
     gene            complement(1413..2111)
                     /locus_tag="PL014_10955"
     CDS             complement(1413..2111)
                     /locus_tag="PL014_10955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT57651.1"
                     /translation="MTTFPRATGGPGAAGPTGGEPQRTVVHLLRHGEVHNPDRVLYGR
                     LPGYHLSELGRHMAELAAEHLGDHDITHVVSSPLERARETAAPIAQGHELEITIDDRV
                     IEAGNAFEGLPVAGGAGLLKHPRLYPKFLNPFRPSWGEPYVEIVERMRLAIIDARAAA
                     RGHEAVIVSHQAPIWMIRLATEGRPLWHNPAKRECSLASLTSLTFLGDELLSLSYSEP
                     SASLLGQAQPGAGA"
     gene            complement(2170..2304)
                     /locus_tag="PL014_10960"
     CDS             complement(2170..2304)
                     /locus_tag="PL014_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HOT57652.1"
                     /translation="MSTPAIVMLVVSVLVIWGGLLFALLHLRRADREDAIEQEMHRDL
                     "
     gene            complement(2301..3863)
                     /locus_tag="PL014_10965"
     CDS             complement(2301..3863)
                     /locus_tag="PL014_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007270952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOT57653.1"
                     /translation="MSTTTSQDEQAEARRGAFSGRRVFILAAIGSAVGLGNIWRFPYV
                     AYENGGGAFMLPYLVALLTAGIPFLLVEYALGHKYRGSAPLSFARLSRRTEGLGWWQV
                     GICFMIAVYYAAVIAWALSYTVFSFTQAWGEDPDAFFFGDYLRAGDPGVTLEFVPGVL
                     VPLAVVWVALVAVMALGVSRGVGATSVIFIPVLIVAFAALVVQALTLPGAAQGLDALF
                     APDWSTLADPAVWAAAYGQIFFSLSIGFGIMITYSSYVHRRTDMSGSALVVGLSNSGF
                     ELLAGIGVFAALGFMAQASGAAVGEVATSGIGLAFIAFPTIVNEAPAGAVIGVLFFAS
                     LVMAGFTSLVSVLEVVISAVRDKFETSRVRATLVVTIPCALISLIGFSTTSGIYVLDI
                     VDHFINRFGILLVAVVSMVVIAWGVRALPRLRDHLNRDGSVPVRGWWIALVSGVTPLA
                     LAFILVRELWAVIEEPYGGYPQWMLVVFGWLAAALVAVAGFAIARVPWRPETSLDVGD
                     RPENDTTARSQP"
     gene            complement(3964..5319)
                     /gene="hemL"
                     /locus_tag="PL014_10970"
     CDS             complement(3964..5319)
                     /gene="hemL"
                     /locus_tag="PL014_10970"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOT57654.1"
                     /translation="MSPDPTPHASRTATSASTASDAPASAALLERARAVTPGGVNSPV
                     RAFRAVGGTPRFMASGRGPYLTDVDGREYVDLVCSWGPMILGHAHPAVLDAVRSAAAR
                     GFSFGTPSENEVLLAEEIVARVDPVQQVRLVSSGTEATMSAIRLARGFTGRSLVVKFA
                     GHYHGHVDSLLVSAGSGLATFALPDSAGVPAEMAAETLVLPYNDVDALEAVFAARGSE
                     IACLVTEAAAGNMGVVPPDPGFTQALRRVTAEHGALLVSDEVMTGFRVSRAGWWGHEG
                     AGVAPDLMTFGKVMGGGFPAAAFGGRADVMALLAPDGPVYQAGTLSGNPIATAAGLAT
                     LRACDDALYARLGEVSSAIADAASAALGAAGVPHRVQWAGSMFSVFFREGAVRTYDDA
                     REQDAAAFGRFFHAMLDAGVYLPPSAFESWFVSGAHDDAAVERVLAALPGAARAAAER
                     G"
BASE COUNT          854 a         1887 c         1973 g          627 t
ORIGIN      
        1 ctcacagcga cacctggaat cggtcaacga gctcgatctt gagccacgtg ttgaggtctt
       61 cccagacccc cgtgaccatg agcagtccga cgagcacgag catcacgccc cccaccacct
      121 ggatggtgcg ctggtgccgc cgcagccacc ccgaggcgcg cccggcccga tccaacccgg
      181 cagcgatgag gatgaacggc agaccgaggc ccaggccgta tgccgtggcg agggtgacgc
      241 tgcgcgccac ctgcgggtcg gcggtcgcgc tggccatgac gagcaccgcc gccagggtcg
      301 ggccggtgca cggagcccag ccgagggcga agacagcccc gagcagcggc gctccggcca
      361 accccgcctt gggtcgccac gcgatcttgg cctcgcgctg accgcccacc ccgaggaaga
      421 ccagccccat gacgatgacg acgatcccgc cgaggcgcat gaggagcacc cggtgctcga
      481 tgagcgctcg acccgctgac gtgacgaaga tcgagccgag cacgaagacg agggtgaagc
      541 ccagcacgaa gagcagcgcc ccggtgacca tgcggctgcg tgagcgctgc gccagcggtt
      601 caccggtcag gccggtgacg tagccgagga agcccggaac gaggggcagg acgcacgggc
      661 tggcgaacga caccaacccg gcgagcaccg ccacggccag ggccagcggc agcgcccccg
      721 acgtgacggt gtccgaggtg agcaccacca tcggggtcac ggccggagcg agtgtcggag
      781 ggagcaccgg catcacgatg gtgaggcggt cgggctcgac tcggcaacgg catcctcgac
      841 cagtgcccgc aacgtgctgg ccgtcaccgg cccgttgacg cgcgcggcga tccgaccctc
      901 ccggtcgagc acgagcgtgg tcggcacggt cggggccttg ccctgcagcg cgaggatcgc
      961 caccccggac tcgtcggaca ggctggggta ggtgatcccg gccctcttct cgaaggctgc
     1021 acccctggcg gggtcctccc ggaagttgat gcccatgaac tgcaccgggg catcctgcgc
     1081 ctcgagctcc gaccagacct tctgcagatc gggggcctcg gcgacgcagg gcgcgcacca
     1141 cgaaccccag acgttgagga cgagcacctc gcccctcgcg gagtcgctcg accagtcggc
     1201 gccgtcgagc agctggccgg agagcctgac cggttcggcc cggtctgccg ccgggatcgt
     1261 ctcgacggca ccgtcaccgg agacgtagcc cttctggtcg ccggccttgg cctgttcggc
     1321 caccgagttc gggtcgctcg tgcacgcagc cgtgccgagc agcagggtgg ccgcggccac
     1381 ccacccggcc caccggacga ggggtctgcc gctcatgcgc ccgccccggg ctgggcctga
     1441 ccgagcaggg atgcggacgg ctcgctgtag gagagcgaca gcagctcgtc gccgaggaac
     1501 gtcagcgagg tgagcgacgc cagggagcac tcgcgcttgg ccgggttgtg ccacagcggg
     1561 cggccctcgg tggcgaggcg gatcatccag atcggtgcct ggtggctgac gatgaccgcc
     1621 tcgtggccgc gggccgcagc ccgggcgtcg atgatcgcga ggcgcatccg ctcgacgatc
     1681 tcgacgtagg gctcacccca tgacggtcgg aacgggttga ggaacttggg gtagagccgt
     1741 gggtgcttga gcagcccggc tcccccggcg accggcagtc cctcgaaggc gttgcccgcc
     1801 tcgatgaccc ggtcgtcgat cgtgatctcg agctcgtggc cctgcgcgat cggggccgcg
     1861 gtctcccgcg cccgctcgag tgggctggac acgacgtggg tgatgtcgtg gtcgccgagg
     1921 tgctcggctg ccagctcggc catgtggcgc cccagctcgg acaggtggta gcccggcagc
     1981 cgtccgtaga gcacccgatc ggggttgtgg acctcgccgt gccgcagcag gtggacgacc
     2041 gtgcgctgcg gctcgccacc ggtgggcccg gccgcgccgg ggccgccagt tgcacgggga
     2101 aaggtcgtca taccggcgat tgtcgcaaac ccggctggat gcccggtcac cgcgcccgcg
     2161 ctcggcccct cagaggtcgc ggtgcatctc ctgctcgatc gcgtcctcgc ggtcggcccg
     2221 gcgcaggtgc agcagggcga acagcagccc gccccagatg acgagcacgg agacgacgag
     2281 catgacgatg gcgggggtgc tcacggctgg ctccttgcgg tggtgtcgtt ctcgggtcgg
     2341 tcgccgacgt ccaggctcgt ctcggggcgc cacggcacgc gggcgatggc gaagcccgcc
     2401 acggccacga gggcggcggc gagccaaccg aagacgacga gcatccactg cgggtagccc
     2461 ccgtagggct cctcgatgac ggcccagagc tcccggacga ggatgaacgc cagggccagg
     2521 ggcgtgacgc cggagacgag ggcgatccac cacccgcgca ccggcaccga gccgtcgcgg
     2581 ttgaggtggt ccctgagccg gggcagggcg cgcacgcccc acgcgatgac gaccatgctg
     2641 acgaccgcga cgagcaggat gccgaaccgg ttgatgaagt ggtcgacgat gtccaggacg
     2701 tagatcccgc tcgtcgtgct gaacccgatg aggctgatca gcgcgcacgg gatcgtgacg
     2761 acgagggtgg cccgaacccg gctggtctcg aacttgtcgc gcaccgcgga gatgacgacc
     2821 tccagcacgc tgacgagcga ggtgaagccc gccatgacga gcgaggcgaa gaacagcacg
     2881 ccgatcacgg ccccggccgg ggcctcgttg acgatcgtgg ggaacgcgat gaaggccagg
     2941 ccgatccccg acgtcgccac ctcgccgacg gccgcaccgg aggcctgggc catgaagccc
     3001 agcgcagcga agacgccgat gcccgcgagc agctcgaagc ccgagttcga caggccgacg
     3061 accagggccg agccggacat gtcggtacgc cggtgcacgt aggaggagta ggtgatcatg
     3121 atgccgaagc cgatggacag cgagaagaag atctggccgt acgcggcggc ccacaccgcc
     3181 gggtccgcga gggtcgacca gtccggcgcg aagagggcgt ccagcccctg ggccgccccg
     3241 ggcagcgtca gggcctgcac cacgagggcg gcgaaggcca cgatgagcac cgggatgaag
     3301 atgacggacg tggcgccgac accgcggctg acgcccaggg ccatcacggc gaccagtgcc
     3361 acccagacga ccgcgagcgg caccaggacg cccggcacga actcgagcgt gaccccgggg
     3421 tcgcccgccc ggaggtagtc gccgaagaag aaggcgtccg gatcctcacc ccaggcctgg
     3481 gtgaacgaga agacggtgta gctgagcgcc caggcgatga cggccgcgta gtagacggcg
     3541 atcatgaagc agatcccgac ctgccaccac cccaggccct cggtacgccg cgacagccgc
     3601 gcgaaggaca gcggcgccga gccacgatac ttgtgcccga gggcgtactc gacgaggagg
     3661 aacgggatgc ccgccgtcag cagcgccacg aggtagggga gcatgaacgc ccctccgccg
     3721 ttctcgtagg ccacgtaggg gaaccgccag atgttgccca ggccgaccgc ggacccgatc
     3781 gccgcgagga tgaagaccct ccggcccgag aaggcgcccc gccgtgcctc ggcctgctcg
     3841 tcctggctcg tcgtcgtact catggaacct ccctgcccgg ggtcatgtgg tcccgccagc
     3901 atcccgcgct cgcggcgagg acgccagccg acgcggcggg ccaggtcgag cccgacgggc
     3961 cgctcagcct cgctcggccg ctgcccgggc cgcccccggc agggcggcga ggacgcgctc
     4021 gacggcagcg tcgtcgtgcg ccccggagac gaaccacgac tcgaaggcgc tcggcggcag
     4081 gtacacgccg gcatccaaca tggcgtggaa gaagcggccg aaggccgcgg cgtcctgctc
     4141 gcgcgcgtcg tcgtaggtgc gcaccgcccc ctcgcggaag aagacggaga acatcgagcc
     4201 cgcccactgg acgcggtggg gcacgccggc tgcgcccagg gcggcggatg ccgcgtcggc
     4261 gatcgcgctc gacacctcac ccaggcgggc gtagagcgcg tcgtcgcagg cccgcagcgt
     4321 ggccagcccg gccgcggtgg cgatggggtt tccggagagg gtgcccgcct ggtagaccgg
     4381 cccgtcgggg gcgagcaggg ccatgacgtc ggcccggccg ccgaacgctg ccgcggggaa
     4441 gccaccgccc atgaccttgc cgaacgtcat cagatcgggg gcgacgccag caccttcgtg
     4501 gccccaccag ccggcgcggc tgacccggaa cccggtcatc acctcgtccg acaccaggag
     4561 cgcgccgtgc tcggccgtca cccggcgcag ggcctgcgtg aaccccgggt ccggggggac
     4621 gacgcccatg ttgccggccg cggcctcggt gacgaggcag gcgatctcgc tgccccgggc
     4681 ggcgaagacg gcctcgagcg cgtcgacgtc gttgtagggc aggacgaggg tctcggcggc
     4741 catctcggcg ggcaccccgg ccgagtccgg cagcgcgaac gtggccagcc ccgagccggc
     4801 gctgaccagc agcgagtcga cgtggccgtg gtagtgcccg gcgaacttca cgaccaggct
     4861 gcggcccgtg aagccgcggg cgaggcggat ggcgctcatg gtcgcctcgg ttccgctgga
     4921 gaccaggcgc acctgctgca cggggtcgac gcgggcgacg atctcctcgg cgagcagcac
     4981 ctcgttctcc gagggggtgc cgaagctgaa cccgcgagcg gcggccgagc ggacggcatc
     5041 cagcacggcg gggtgcgcgt gaccgaggat catcggcccc cagctgcaca cgaggtccac
     5101 gtactcgcgg ccgtcgacgt cggtgaggta cgggccgcgc ccgctcgcca tgaagcgggg
     5161 ggtgccgccg accgcgcgga aggcgcgcac cggggagttg accccgccgg gggtgacggc
     5221 gcgggcgcgc tcgaggaggg cggcggatgc cggggcgtcg ctcgcggtgg acgcgctggt
     5281 cgcggtgcgg ctggcgtggg gtgtcgggtc agggctcacc ccaccattgt ccgacacccc
     5341 t
//
LOCUS       DAOSOC010000168         5326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_15542_length_5326_cov_5.641814, whole genome shotgun sequence.
ACCESSION   DAOSOC010000168 DAOSOC010000000
VERSION     DAOSOC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15542_length_5326_cov_5.641814"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1177
                     /locus_tag="PL014_10975"
     CDS             65..1177
                     /locus_tag="PL014_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann fold nucleotide-binding protein"
                     /protein_id="HOT57655.1"
                     /translation="MQEIEDLDGLDAVLSTPRPLAGLRFQDLDLTGHEAPLLERTDLE
                     GLVVLGGRVSADLARHLRQHGALVFPTDPGVPINPYRATLYQPHELYTGLSEHGYDAT
                     PDALAYHWSRDGDSHHDAFVTLLRAIHDDSMSDALSEVIGSSAVVGVMGGHALERGTA
                     GYTGAAHLGHALAEAGFVVATGGGPGAMEAANLGAYAAFADAVDGAVERLAGVPSFRS
                     GIGPWAQVALDLHDDLLAARGGAGAVRSIGIPTWFYGHEPPNVFCDGIAKYFSNAIRE
                     DGLLARCDAGIVVLPGVAGTVQEVFQAATRLFYATPDAVLPLLVLVGRDHWTNTIPVW
                     PALQALAVGRPMEQRLHLVDSPAEAAALVTGNSATL"
     gene            complement(1235..1570)
                     /locus_tag="PL014_10980"
     CDS             complement(1235..1570)
                     /locus_tag="PL014_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57656.1"
                     /translation="MQHGYQRALNIIGLVVAAGLNVAVVSFYVLWFIADSDAINRMET
                     ATSVDPTVLLPNATLLWLAANASLVALLALDGVIVAMTMAARSRRRLAGQDPDTAEEL
                     STPISSRPG"
     gene            complement(1580..2575)
                     /locus_tag="PL014_10985"
     CDS             complement(1580..2575)
                     /locus_tag="PL014_10985"
                     /inference="COORDINATES: protein motif:HMM:NF022240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="HOT57657.1"
                     /translation="MSVYSLFRAVVGATILAALLPVSAAHSQELGDEAVVRLVEDAAP
                     IRVPVLSVQEFDEGTTAVPAVTSNGRVSVPESDEGLIVLEAPETPRQPGSRIGVALPA
                     EAAAGHGTVTESGAVVYSGDSDRVDVVVQPVEDGSVRISTVVNESSAPHAFTYKLSLP
                     GAASLELLGDGSIIIMRGGRHIGGVAAPWAVDAAGRDVATHFEIDEHSFTQVVEPTAS
                     ATYPIVADPYLGISLISKAVWARDLWQYSPTLKVYPTWYGRYGPAAARWAAWSETLNK
                     TPRSGWPNPDTASMKNQFYCHFDVVRLRAPNKEYWGLDSKIPNRGYWGFVNNSCN"
     gene            complement(2878..3441)
                     /locus_tag="PL014_10990"
     CDS             complement(2878..3441)
                     /locus_tag="PL014_10990"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HOT57658.1"
                     /translation="MSLRDLRLRRGWSQEHLAEASGVSVRTIQRIEGGQPPSPFTASA
                     LAVALDVDPGSFGEPNTEPADGERADALEPAPALTFVEAVRQGVRRWGDFDGRASRSE
                     FWFFFLAVSVVVGALAALDDRLGAAALVLCLVPLAAAGTRRLRDAGQSPWWLLFAIVP
                     MGFVVLLTLMVMPGKAIEEVATPQNSR"
     gene            complement(3556..4734)
                     /locus_tag="PL014_10995"
     CDS             complement(3556..4734)
                     /locus_tag="PL014_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57659.1"
                     /translation="MHRRAPALLAALALLLAGCGSEADLPANLKSADTHASAHATAPS
                     ATVEPVKETPLRKGERFQEIRLPAKYTPKAPEGTGTDDYRCFLVDPGFETDQLVSGVQ
                     VLLDNAPLVHHVIVSKVEPDEVAKAEKIDAQTPGEGWTCFGGAGISGIAGGNLDKADW
                     VGAWAPGGGERVMAEDIGIPLKAGSQLVVQMHYNLLAGDGADRSAVLLRLSEAKGSDK
                     QALQTMLLPAPVELPCRDNRTKGMCSRALAVADLKKRFAEDPATADLLHLLCGPLKTG
                     PVQSCTRPIREEVTIRAVSGHMHLLGRAITVDVNKGTPKAKRVLDIPVWNFDDQGSIP
                     LETPLDVGPGDTITVTCTHDQELRDLLPAFEGTEDRYVAWGEGTTDEMCLGIVLMTER
                     "
     gene            complement(5002..>5326)
                     /locus_tag="PL014_11000"
     CDS             complement(5002..>5326)
                     /locus_tag="PL014_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57660.1"
                     /translation="RYHEATGRRVSIEYALIRDINDQAWRADLLGQKLRQRGRGWVHV
                     NPIPLNPTPGSKWTASRRGVEQQFVERLRAHGIPTTVRDTRGSDIDGACGQLAAATSG
                     APERG"
BASE COUNT          824 a         1881 c         1818 g          803 t
ORIGIN      
        1 cgaccggctc accgcccact gacctttctc cccccgaggg tgacgggtcg gctacgttcg
       61 gggcgtgcag gagatcgagg acctcgacgg cctggatgcc gtgctgagca ctccgcgccc
      121 gctcgccggg ttgcgcttcc aggacctcga cctgaccggc cacgaggctc cgctgctgga
      181 acgcaccgac ctcgaggggc tcgtcgtcct cggtggacgg gtcagcgccg acctcgctcg
      241 acacctgcgc cagcacgggg ctctggtctt cccgaccgac cccggggtgc cgatcaaccc
      301 ctaccgggcg acgctctacc aaccgcacga gctctacacg gggctcagcg agcacgggta
      361 cgacgccacg cccgacgccc tggcctacca ctggtcacgg gacggtgact cgcaccacga
      421 cgccttcgtc accctgctgc gggccatcca cgacgactcg atgagcgacg cgctgagcga
      481 ggtcatcggc agctcagcgg tcgtcggggt catgggtggg cacgcgctcg aacggggaac
      541 ggcgggctac accggggccg cccacctcgg ccatgccctg gccgaggccg ggttcgtcgt
      601 ggccacgggg ggcggaccgg gcgcgatgga ggctgccaac ctcggcgcgt acgcggcatt
      661 cgctgatgcc gttgacggcg cagtagagcg gctggccggc gtcccgtcgt tccgctcggg
      721 gatagggccc tgggcccagg tggcgctcga cctgcacgac gacctgctcg ccgcacgcgg
      781 cggcgcgggc gcggtgcgca gcatcggcat ccccacgtgg ttctacggcc acgaaccgcc
      841 caacgtcttc tgcgacggca tcgccaagta cttctcgaac gcgatccgcg aggacggcct
      901 gctcgcgcgc tgcgatgccg ggatcgtcgt gctccccggc gtggccggca ccgtgcagga
      961 ggtcttccag gcggcgacgc ggctcttcta cgccacgccg gatgccgtgc tccccctcct
     1021 cgtgctcgtc ggccgggatc actggacgaa cacgatcccc gtgtggccgg cgctgcaggc
     1081 cctggccgtg gggcgcccga tggagcagcg gctgcacctc gtcgactccc ccgccgaggc
     1141 cgccgccctg gtcaccggca actcggccac gctgtgacat gcagaacggg cagcatgctg
     1201 cccgttctgc atgtcacagc ccacgacgaa cgtttcagcc aggccgggat gagatcgggg
     1261 tgctcaactc ctcagcagtg tcggggtcct ggccggcgag acgacgcctt gaccgcgccg
     1321 ccatggtcat tgccacgatc accccatcga gtgcgagcag ggccacgagc gacgcattcg
     1381 cagcgagcca cagaagagtc gcgttgggca acaggacagt cgggtcgacc gaggtggccg
     1441 tctccatccg attgatggcg tccgagtcgg cgatgaacca cagcacatag aaggacacga
     1501 ccgcgacgtt gaggccggca gccacgacga gtccaatgat gttgagggcc cgctggtaac
     1561 catgctgcat gattgctcct tagttgcagc tgttattgac gaagccccaa tacccgcggt
     1621 taggaatctt cgagtccaag ccccagtatt ccttgttcgg tgcgcgaagt cggacgacgt
     1681 cgaagtgaca gtagaactga ttcttcatcg acgcggtgtc tgggttgggc caacccgagc
     1741 gcggggtctt gttcagcgtt tcgctccacg cggcccagcg cgcagccgcg ggaccatacc
     1801 ggccgtacca cgtcgggtac accttgagcg tggggctgta ctgccacagg tcccgggccc
     1861 acaccgcctt ggagatgaga gagatcccca ggtacgggtc cgccacaatc gggtaggtgg
     1921 ccgaggccgt gggttcgaca acctgcgtga atgagtgttc gtcgatctca aagtgcgtcg
     1981 cgacatcgcg accggcggca tccactgccc acggtgccgc gacgccgccg atgtgccgac
     2041 cacccctcat gatgatgatg gaaccgtccc ccaacagttc caacgaggcc gcgccgggaa
     2101 ggctcagctt gtaggtgaaa gcgtgtggtg ccgacgactc gttgacgacg gtgctgatgc
     2161 gaaccgaacc gtcctccacg ggctggacga cgacatccac ccggtcgcta tcgccgctgt
     2221 agacaacagc cccagattcg gtgacggtgc cgtggccggc ggctgcctca gccgggagcg
     2281 cgacaccgat cctggaccca ggctgccggg gcgtctcggg tgcctcaagc acaatgagcc
     2341 cttcgtcaga ctccggcacc gaaacgcgcc cgttcgaggt gacggctggc acagctgtcg
     2401 tcccctcgtc gaactcttgt acggaaagga ccggaactcg tatcggcgcg gcgtcctcta
     2461 ccaacctgac aacggcctcg tccccgagtt cctgcgaatg ggccgccgat acaggcagca
     2521 gagccgccag gatcgtcgcc cccaccacag cacggaacaa gctgtacacg ctcattgata
     2581 tccctcctgg gaccaacccc ctgttggtcc ggcgccaacc gtagcgcagc gaatcggccg
     2641 cgtccattgg agcgcaatct actctcgcag tcgggcttcc gcatccgaga ggacgggctg
     2701 ccgcgcgctg aggcgccagg atcgtcgtgc tgcccggcac ggcaggccgc accacccgtg
     2761 tggcgggcgc tccaagcgtt ggccgctggg cgctgggcgg agcagcggct gcacctcgtc
     2821 gactcccccg ccgacgccgc cgccctcttc gctcggtagg accgcggcgc ccatgggtca
     2881 ccgactgttc tggggcgtcg ccacctcctc gatcgccttt ccgggcatca ccatgagggt
     2941 cagcaggacg acgaacccca tcggcacgat cgcgaagagc agccaccagg ggctctgacc
     3001 tgcgtcccgc aaccggcggg tcccggccgc ggcgaggggc acgaggcaca gcacgagggc
     3061 ggcagccccg agccggtcgt ccagcgcagc gagggcaccg acgacgacgg acacggcgag
     3121 gaagaagaac cagaactccg agcgactggc ccggccgtcg aagtcgcccc accggcggac
     3181 cccctgacgc acggcctcga cgaaggtcag cgccggcgcc ggctcgaggg cgtcagcgcg
     3241 ttcaccgtcg gcgggctcgg tgttcggttc gccaaaggac ccgggatcga catcgagtgc
     3301 caccgcgagg gcggaggccg tgaaggggct cggtggttgg ccaccctcga ttcgctggat
     3361 ggtgcgcacg ctcacgccgc tggcctcggc caggtgttcc tggctccagc cgcgccggag
     3421 ccggaggtct cgaagactca cgtcaccagt gtcgccccac cacgggcgcc accaccacga
     3481 cgacaacccg ccatcctgcc gccatcctcc cgccatcggc ccctgcacgg gcgtcggcag
     3541 tcatcagcag gagcgtcagc gctcggtcat gaggacgatt cccaggcaca tctcgtcggt
     3601 cgtgccctcg ccccacgcga cgtagcggtc ctcggtgccc tcgaacgcgg ggagcaggtc
     3661 gcgcagctcc tggtcgtggg tgcaggtcac cgtgatggtg tcaccgggac cgacgtcgag
     3721 aggggtctcc agggggatgc tgccctggtc gtcgaagttc cagacgggga tgtccagcac
     3781 gcgcttggcc ttcggcgtcc ccttgttgac gtccaccgtg atcgcccggc cgagcaggtg
     3841 catgtgcccg gagacggcgc ggatcgtcac ctcctcacgg atcggtcggg tgcagctctg
     3901 caccggcccg gtcttcagcg gcccgcagag caggtgcagc aggtcggcgg tggcggggtc
     3961 ctcggcgaag cgcttcttga ggtcggcgac cgcgagtgcg cggctgcaca tccccttcgt
     4021 gcggttgtcg cggcagggca gctcgaccgg agcaggcagg agcatcgtct gcagggcctg
     4081 cttgtcactg cccttggcct cagacagcct caggagcacg gcactgcgat cggcgccgtc
     4141 gcccgcgagg aggttgtagt gcatctggac gacgagctgc gagccggcct tgagcgggat
     4201 gccgatgtcc tcggccatga cccgctcgcc cccaccgggt gcccacgcgc cgacccagtc
     4261 ggccttgtcg aggttgccgc cggcgatgcc cgagatgcct gcgccaccga agcaggtcca
     4321 gccctcaccg ggggtctgcg cgtcgatctt ctcggccttc gcgacctcat ccggctcgac
     4381 cttggagacg atgacgtggt gcaccagcgg tgcgttgtcg agcaggacct ggacgccact
     4441 gacgagctgg tcggtctcga agccggggtc gacgaggaag caacggtagt cgtcggtgcc
     4501 ggtgccctca ggcgccttcg gggtgtactt cgccgggagg cggatctcct ggaagcgctc
     4561 gcccttgcgc agaggcgtct ccttgaccgg ctcgaccgtg gcgctcggcg cggtggcgtg
     4621 ggcgctcgca tgcgtgtcgg cggacttcag gttcgccggc aggtcggcct cgctgccgca
     4681 cccggcgagc agcagggcaa gggcagccag aagtgccggg gcgcgacgat gcatcggcca
     4741 agcgtacccg cgggtacgag ggtgcagggc gcttgtcgag ccccggcatc cgaccagggt
     4801 ggaccttgcc cgggcactgg tcgaaccagt gagcccgggt accagtgctg tcaggggccc
     4861 ttgcgcccga caagggagcc cttgcgcgcc gcaaggacgc ctgacagcac cccccgacgc
     4921 cctctcagca ccgagcaccg agcaccgagc accgagcacc gcgatgccac gggcccccgt
     4981 cagaaccgcg cgcgacgacg ctcagccgcg ttcgggggct cccgaggtcg cggcagccag
     5041 ctgaccgcag gcgccgtcga tgtcggaccc gcgcgtgtca cgaacggtcg tcgggatgcc
     5101 gtgcgcccgc agccgctcga cgaactgctg ctcgacgcct cgtcgggatg ccgtccactt
     5161 cgacccgggg gtcgggttca gcgggatggg gttgacgtgg acccagcccc ggccgcgctg
     5221 gcgcagcttc tggccgagca ggtccgcccg ccacgcctgg tcgttgatgt cgcggatgag
     5281 ggcgtactcg atgctgaccc ggcgcccggt ggcctcgtgg tagcgg
//
LOCUS       DAOSOC010000169         5301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_15641_length_5301_cov_10.180900, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000169 DAOSOC010000000
VERSION     DAOSOC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15641_length_5301_cov_10.180900"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..612
                     /locus_tag="PL014_11005"
     CDS             43..612
                     /locus_tag="PL014_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOT57661.1"
                     /translation="MPRKGKDWARSRGIGPGEREWNEPWERLRHHLMERHGERVDVVR
                     RLERPDPDDLRNLAIFARQASAAMDADLARLLGTMTPQLTPLELDTLRRIADKPSCGV
                     NLAEYLRISPVRVCRILNRLDEAGLTVRGPTYLDARVRRAEVTDEGRDYLHSVKDQLQ
                     DLAHQWLDDLEDGADRALVPLLATLADLT"
     gene            complement(640..2103)
                     /locus_tag="PL014_11010"
     CDS             complement(640..2103)
                     /locus_tag="PL014_11010"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT57662.1"
                     /translation="MSPPHARPLDVDVLVVGAGIAGVDVGARLGMHCPGTAWAVLESR
                     DAVGGTWDLFRYPGIRSDSDMYTLGFPFRPWRGEASLASGEQIRQYVQDTAREFGVTE
                     RIHHGQRVERLDWSSDDARWTVTARTDAGTVRHTARLVYLATGYFSYEGGHVVDFPGQ
                     ELFAGPVIHPQAWPDDLSVQGKRVVVIGSGATAVTLVPALVEAGACHVSMLQRSPSYV
                     ASVPSRDRVATRVRQVMPDAVAHRVVRAKNVALSTLGYQVLRRYPDAGRRLLRRRMEQ
                     ALPAGYPVELHFTPTYDPWDQRLCAAADGDLFTVLGDGRASVVTASIAAFEGDGIRLA
                     GGEHVPADVIVTATGLQMEFGGGARVFIDGVEVDVSSGHVYKGLMLSDVPNLAMAVGY
                     TNASWTLRADLSARWFCSLVRYLDRRGLSVATPRYDEPDAVDAPMLDLTSGYVQRAAH
                     LLPKQGRRRPWRVVQSYAYDLAVTRLGRIDDGHLELR"
     gene            2201..2692
                     /locus_tag="PL014_11015"
     CDS             2201..2692
                     /locus_tag="PL014_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOT57663.1"
                     /translation="MTTLSDFSATTLTGQERDLSDYAGKVVLVVNTASKCGLTPQYEG
                     LEALYQEFKDDGLVVLGFPCNQFGSQEPGTEDEIGEFCQVNYGVSFPMFAKVDVNGDD
                     THPLFEWLKSETKGLLGGRVKWNFTKFLVGRDGQAISRYAPTTEPGDLADDIRAALAA
                     KAA"
     gene            2689..3837
                     /locus_tag="PL014_11020"
     CDS             2689..3837
                     /locus_tag="PL014_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT57664.1"
                     /translation="MSPWLILIPLGVFGGFIAFVWHRLAIAPGWRPRWARYAVAAALV
                     VLTAFAFAGFDVWGGSFTPAQMRPVAWLGQAFLASCLYLFLGLVPVWLASVAIWLLRW
                     RHDDHGRSARRRLNAIASPLVAAVAVGVTAYGAVEAATPSVSRFELASPQLPAEFDGA
                     RVALVTDVHAGAVRSAAFTQQVVDLVNEQEPDLIVLAGDLVDGKAARYAPELAPLADL
                     RAPLGVFATTGNHEMFEDTVNWVSAFEDVGLVVLGNESVPLERGGATMVLAGVHDAIG
                     EGVFAPDYDAALAGVDPGGFTLLAAHQPLQAFDVEGRGVDLQVSGHTHGGQMWPINYL
                     VPLQQPMLEGRGDVAGTPVITSRGAGAWGPAIRVAAPPEVPIITLKRS"
     gene            3885..4490
                     /locus_tag="PL014_11025"
     CDS             3885..4490
                     /locus_tag="PL014_11025"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOT57665.1"
                     /translation="MSEPGVVIGDDGLARPAWASVDPLLRHYYDTEWGMPVRDERGMF
                     ERLSLEAFQSGLSWATILRKRPAFRKAFAGFDPEVVAAFDDRDVERLMADAGIVRNRA
                     KVAATITNARATLALRDDPDGDLATFVWSFQPPQTPRPQTMADVPTSAPESVALSKAL
                     KRKGFTFVGPTTMWALMEAIGIVDTHLLGSHRRGTSGVWPH"
     gene            complement(4487..>5301)
                     /locus_tag="PL014_11030"
     CDS             complement(4487..>5301)
                     /locus_tag="PL014_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOT57666.1"
                     /translation="VPGVEGEQFGVVDHQRGTGLLVVSLAFVLALLAFGRWRGLSALA
                     GLGVTFLVLVFFVVPAILGGESPMLVALVGSAAITLTVLYLTHGITMTSTVAVLGTLA
                     SLVLTGVLAAVSVSALRLSGITDDVSSSVSDRYGITMSGLLVASIIIGSVGVLDDVTV
                     TQASIVAELAEANPTYGVGQLYRSASRVGRSHIASVVNTIVLAYAGASLPLLILVAAD
                     NPSFADVVTTQFISQEIVRSIVATLGLIAAVPMTTALAAYAARASNAQPVDG"
BASE COUNT          784 a         1979 c         1812 g          726 t
ORIGIN      
        1 gtgatgatgg cccgacgcac aacgaaccgg ggggatcgga gcatgccacg caagggcaaa
       61 gactgggcac gcagccgggg catcggaccc ggcgaacgtg agtggaacga gccgtgggag
      121 cggctgcgcc accacctcat ggaacgccac ggcgaacgcg tcgacgtcgt gcgccgtctg
      181 gagcggccgg accccgacga cctccgcaac ctcgcgatct tcgcgcgtca ggccagcgcc
      241 gccatggatg ccgacctggc ccgcctcctc ggcaccatga cccctcagct caccccgctc
      301 gagctcgaca ccctgcgacg catcgccgac aagccgtcct gcggcgtcaa cctcgccgag
      361 tacctgcgca tctcccccgt ccgggtctgc cggatcctca accggctcga cgaggcgggg
      421 ctgaccgtcc gtggcccgac ctacctcgac gctcgggtgc gccgcgccga ggtgaccgac
      481 gagggccggg actacctcca cagcgtcaag gaccagctcc aggacctcgc ccaccagtgg
      541 ctcgacgacc tcgaggacgg cgcagatcgg gccctcgtcc ccctgctggc caccttggcc
      601 gacctcacct gacccctcgg caggacctca ccagatcgct cacctgagct ccaggtgccc
      661 gtcgtcgatc cggccgagcc gcgtgaccgc gaggtcgtag gcgtagctct gcaccacccg
      721 ccacgggcgg cggcgcccct gcttcggcag caggtgcgcc gcccgctgca cgtaccccga
      781 cgtcaggtcg agcatcggcg cgtccacggc atccggctcg tcgtagcgcg gggtcgcgac
      841 cgacaacccg cgccggtcca ggtaccgcac gagcgaacag aaccaccggg ccgacaggtc
      901 ggcgcgcagg gtccagctgg cgttggtgta ccccacggcc atcgcgaggt tcggcacgtc
      961 ggagagcatc agccccttgt agacgtggcc gctcgacaca tcgacctcca ccccgtcgat
     1021 gaacacccgc gctcccccgc cgaactccat ctgcagcccg gtcgccgtca cgatgacgtc
     1081 tgccggcacg tgctcgccgc cggcaagtcg gatgccgtcg ccctcgaagg cggcgatgct
     1141 cgccgtcacg acgctggccc gcccgtcgcc gagcacggtg aacaggtcgc cgtccgccgc
     1201 ggcgcacagg cgctggtccc acgggtcgta cgtcggtgtg aagtgcagct ccaccgggta
     1261 cccggcagga agcgcctgct ccatccgtcg acgcaggagg cgacgcccgg catccgggta
     1321 ccgccgcagc acctggtacc cgagggtgga cagcgcgacg ttcttggcgc gcacgacccg
     1381 gtgcgccacg gcatccggca tcacctgccg tacccgggtg gccacgcggt cgcggctggg
     1441 caccgacgcc acgtagctcg ggctgcgctg cagcatgctc acgtggcagg cccccgcctc
     1501 gaccagtgcc ggcacgaggg tcacggccgt cgcgccgctg ccgatgacga cgacccgctt
     1561 gccctgcacc gacaggtcgt ccggccacgc ctgcgggtgg atgaccgggc cggcgaacag
     1621 ctcctgaccg ggaaagtcga cgacgtgccc gccctcgtag gagaagtagc ccgtcgcgag
     1681 gtagaccagc cgcgcggtgt gccgcaccgt cccggcatcc gtgcgcgccg tcaccgtcca
     1741 gcgcgcgtca tccgacgacc agtccagccg ctcgacccgc tgcccgtggt ggatgcgctc
     1801 ggtgaccccg aactcacgcg cggtgtcctg cacgtactgc cggatctgct cccccgacgc
     1861 cagggacgcc tcccctcgcc agggccggaa cgggaagccg agggtgtaca tgtcggagtc
     1921 ggagcggatg ccggggtagc ggaacaggtc ccacgtgccg ccgacggcgt cccgcgactc
     1981 gagcaccgcc cacgcggtgc ccggacagtg catgcccaac cgggcgccga cgtcgacccc
     2041 ggcgatgcca gcgcccacga cgaggacgtc gacatcgagg ggccgggcgt gcggtggtga
     2101 caccgggtca gcgtattccg cctcggggcg tgtcaaacct cgcgcccgcc gggggcctca
     2161 cgactgcgtc cgcgtcggtg gcccgtgtga cgatgacctc atgaccactc tctccgactt
     2221 cagcgccacg accctcaccg gccaggagcg cgacctctcc gactacgccg gcaaggtcgt
     2281 cctcgtcgtc aacaccgcca gcaagtgcgg cctgaccccc cagtacgaag gcctcgaagc
     2341 cctgtaccag gagttcaagg acgacggcct cgtcgtgctc ggcttcccgt gcaaccagtt
     2401 cggcagccag gagcccggca ccgaggacga gatcggtgag ttctgccagg tgaactacgg
     2461 cgtgagcttc ccgatgttcg cgaaggtcga cgtgaacggc gacgacacgc acccgctctt
     2521 cgagtggctg aagtcggaga ccaagggcct gctcggtggc cgggtgaagt ggaacttcac
     2581 gaagttcctc gtcggccgtg acgggcaggc catcagccgg tacgcgccga cgaccgagcc
     2641 cggcgacctc gccgacgaca tccgcgcagc cctcgccgcc aaggcggcat gagcccctgg
     2701 ctcatcctca tcccgctcgg ggtcttcggg ggtttcatcg cctttgtgtg gcaccgcctg
     2761 gcgatcgccc ccgggtggcg cccccgctgg gcgaggtatg ccgtggcggc tgcgctcgtc
     2821 gtgctcacgg cgttcgcgtt cgccgggttc gacgtctggg gtgggtcctt cacgcccgcg
     2881 cagatgcggc cggtggcctg gctcggccag gcgttcctcg cgtcctgcct gtacctgttc
     2941 ctcgggttgg tcccggtgtg gctcgcgagc gtcgcgatct ggctcctgcg ctggcggcac
     3001 gacgaccacg gccgctccgc caggcggcgc ctgaacgcga tcgcgtcgcc gctggtggct
     3061 gccgtcgcgg tgggggtcac cgcctacggc gccgtcgagg cggccacccc gagcgtgagc
     3121 cggttcgagc tggcgtcccc gcagctgcct gccgagttcg acggggcccg cgtcgcgttg
     3181 gtcaccgacg tgcacgcggg ggcggtgcgc agcgccgcct tcacccagca ggtcgtcgac
     3241 ctcgtcaacg agcaggagcc cgacctcatc gtgctcgccg gcgacctcgt cgacggcaag
     3301 gccgcgcgct acgcccccga gctcgccccg ctcgctgacc tgcgcgcccc gctgggtgtc
     3361 ttcgcaacga cgggcaacca cgagatgttc gaggacaccg tcaactgggt gtcggcgttc
     3421 gaggacgtcg gcctggtcgt gctgggcaac gagtcggtgc cgctggagcg tggtggggcg
     3481 accatggtgc tggcgggcgt tcacgacgcc atcggcgaag gggtcttcgc gccggactac
     3541 gacgcggccc tggccggcgt cgaccccggg ggcttcacgc tgctcgccgc ccaccagccg
     3601 ttgcaggcct tcgacgtcga gggtcgcggc gtcgacctcc aggtctccgg gcacacccac
     3661 ggcggtcaga tgtggccgat caactacctc gtgccccttc agcagccgat gctcgagggc
     3721 cgcggcgacg tcgccggcac tcccgtcatc acctcgcgtg gtgccggggc ctgggggccg
     3781 gcgatccggg tggctgctcc cccggaggtc ccgatcatca ccctgaagcg gtcgtgagcg
     3841 gatgccgcgc ctgaccggcg gccggccgtg accggcatcc ggccgtgagc gagcccggcg
     3901 tcgtcatcgg cgatgacggg ctcgcccggc ccgcgtgggc gtcggtggac ccgttgttgc
     3961 gtcactacta cgacaccgag tgggggatgc cggtgcgcga cgaacgcggg atgttcgagc
     4021 ggctgtccct cgaggccttc cagtcggggc tgtcctgggc gacgatcctg cgcaagcgac
     4081 ctgccttccg caaggcgttc gccggcttcg acccggaggt cgtcgccgcg ttcgacgacc
     4141 gcgacgtcga gcgcctcatg gctgacgccg ggatcgtccg caaccgcgcg aaggtcgcgg
     4201 cgacgatcac caacgcccgg gccaccctcg cgctgcggga tgaccccgac ggcgacctgg
     4261 ccacgttcgt gtggtccttc cagccgccgc agacaccgcg gccccagacc atggccgacg
     4321 tgccgacctc ggccccggag tcggtggcgc tgtccaaggc cctcaagcgc aagggcttca
     4381 ccttcgtcgg gccgacgacg atgtgggcgc tcatggaggc catcggcatc gtcgacaccc
     4441 acctgctcgg ctcccaccgg cgcggcacca gcggcgtctg gccgcactag ccgtcgacgg
     4501 gctgcgcgtt cgacgcgcgg gcggcgtagg cagccagggc cgtggtcatc gggacggcgg
     4561 cgatgaggcc cagggtcgcc acgatgctgc gcacgatctc ctgggagatg aactgcgtgg
     4621 tcaccacgtc ggcgaacgag gggttgtcgg cggccacgag gatgagcaac ggcagcgacg
     4681 cacccgcgta ggccagcacg atcgtgttga cgaccgaggc gatgtgcgac cggccgacac
     4741 ggctcgccga gcggtacagc tgaccgaccc cgtacgtcgg gttggcctcg gccagctcgg
     4801 ccacgatgct cgcctgggtc acggtcacgt cgtcgagcac gccgacggag ccgatgatga
     4861 tgctcgccac gagcagaccg gacatggtga tgccgtagcg gtcgctcacc gacgacgaca
     4921 cgtcgtccgt gatgccgctc agcctgaggg cactgaccga gaccgctgcg agcacccccg
     4981 tgagcaccag gctggccagc gtgccgagga cggccaccgt cgacgtcatc gtgatgccgt
     5041 gggtcaggta gagcaccgtg agcgtgatcg cggccgaccc gacgagcgcc acgagcatgg
     5101 gcgactcccc accgaggatc gccggcacga cgaagaacac caggacgagg aacgtcacgc
     5161 cgagacccgc caacgcggac agcccgcgcc accgcccgaa cgccaggagc gccagcacga
     5221 aggccaggga caccacgagc agcccggtgc cgcgctggtg gtccacgacc ccgaactgct
     5281 ccccctccac acctggcacc g
//
LOCUS       DAOSOC010000170         5209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_16019_length_5209_cov_14.244470, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000170 DAOSOC010000000
VERSION     DAOSOC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16019_length_5209_cov_14.244470"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..2621
                     /gene="valS"
                     /locus_tag="PL014_11035"
     CDS             27..2621
                     /gene="valS"
                     /locus_tag="PL014_11035"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOT57667.1"
                     /translation="MPDKPSLDGLEDKWAAVWQEQDTYAFDREAALAGPREAVFAIDT
                     PPPTASGSLHMGHVFSYTHTDCVARYKRMAGFNVFYPIGWDDNGLPTEKRVQNYYGVR
                     GDAALHHDPDFVPPFRGDARSTKAADEMPVSRQNFIELCDELTVRDEEAFESLFRRIG
                     HSYDWSITYRTIDDHSRATAQQAFLRNLARGEAYQAEAPGLWDVTFQTAVAQAELEAR
                     DYPGHYHRVAFHGPHGPVHIETTRPELIPSCVALIAHPDDERYQGLFGTTVTTPLFGV
                     EVPVLAHPGAEMDKGAGIAMCCTFGDLTDVIWWRELQLPTRSVITRSGRLQGETPAWL
                     EGTPGEALYAEQLAGKTPFGARAALVDALRESGDLDGEPKATQRMANFYERGEKPLEI
                     VTSRQWYIRNGGRDAELNAALIARGEQIDFHPAFMRSRYANWVSGLNGDWLVSRQRFF
                     GVPIPVWYPLDADGEPVYDTPIVPDESALPVDPAANPPAGYTEDQRGVPGGFIGDPDV
                     LDTWATSSLSPQIAGGWRNADGTGTDPLFDKVFPMDVRPQGHDIIRTWLFSTVVRAHF
                     EHGSVPWTDAAISGWILDPDRKKMSKSRGNVVTPEDLLKEHSADAVRYWAASGRLGTD
                     AAYDTGQIKVGRRLAIKVLNASKFALSFGSVEGDLAAQVTEPIDLSLLAGLRDVVAKA
                     TAGFEAWDFTRALEVTEQWFWSFCDDYLELVKDRAYGAQGDAGAASARATLRISLDVV
                     LRLFAPVLPYVTEEVWSWTHDGSVHRASWPTVDEVPGSGDPAVLGSVGAALAVVRKAK
                     SEAKVGMRAEVPSMTLVGPDAVHAHVRSAEGDLRAAGKLTGILDYAEGDEVAARDIEL
                     VAVPKPTA"
     gene            complement(2745..3416)
                     /locus_tag="PL014_11040"
     CDS             complement(2745..3416)
                     /locus_tag="PL014_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57668.1"
                     /translation="MPYRLRAGDTGPVQQSPVTCGSACLTVARMHVDPHFASWVRTGF
                     PNPPGSPQGATPGERFAAYERIVMRRTNSLFPGSGRLNVPWPRQLGTPPWGARRELEF
                     GAARRGTQYTIDVLRADSRTDRVLAFDTLVEVVAEGEPALLYVGNATLPRHVVLILPG
                     DGDRMLDVYDPGSGRVTHLRRDVVVDAQLALSGWDVPWFAVRPTGLRPVIRGGYAVTP
                     DAAPA"
     gene            complement(3547..4557)
                     /locus_tag="PL014_11045"
     CDS             complement(3547..4557)
                     /locus_tag="PL014_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT57669.1"
                     /translation="MARYAGVSRATVSRVVNGSTTVDPALREIVNQAVDDLNYVPNAA
                     ARALMTRRTDSVTLVAAEGDDRVFGDPFFAQIVRGASQELTRAGVQLSLSMTQSDLQI
                     TSLSRYLAGGHTDGVLLISEHGGHRLAEKLVEAGIPVVIGGRPLDPAVEVPYVDNDNV
                     AGGRLAAEHLRARGCRRVATIAGPADMSAGCDRLEGFRQGLGPDFREDLVEHGLFTMA
                     SGEAAATRLFERSPDIDGIFVASDLMALGAISVIRKLGKRVPDDVAIVGFDDIELASM
                     AEPSLTTIRQRTENQGRLMARLLLRQLGHEVDDVESEPAPSTPDGRGIVQPVDLIERE
                     SA"
     gene            complement(4601..>5209)
                     /locus_tag="PL014_11050"
     CDS             complement(4601..>5209)
                     /locus_tag="PL014_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT57670.1"
                     /translation="PQGGRKHPHQEFIQIDTTNVLFIVGGAFAGLEKLIESRNGKKGL
                     GFGSELHTPKDLAESIHEVMPEDLMKFGLIPEFIGRVPVITTVAPLDNDALVRILTEP
                     RNALIKQYQKMFEIDGVVLEFTEDAVAAVADQALARGTGARGLRAIMEEVLLPTMFDV
                     PSDDEIAKVVVTSEVVRKNVLPTIVRHSDDPTRKRAPRKRSA"
BASE COUNT          775 a         1870 c         1812 g          752 t
ORIGIN      
        1 ccctttcgcc atatcgtgct gcctccgtgc cggacaagcc gtccctcgac ggcctcgagg
       61 acaagtgggc ggccgtatgg caggagcagg acacctacgc gttcgaccgc gaggcggccc
      121 tcgccggccc ccgcgaggcg gtcttcgcca tcgacacccc gccgcccacg gcatccgggt
      181 cactgcacat ggggcacgtg ttcagctaca cccacaccga ctgcgtggcg cggtacaagc
      241 ggatggccgg gttcaacgtc ttctacccga tcggctggga cgacaacggg ctgcccaccg
      301 agaagcgggt gcagaactac tacggcgtgc gcggtgacgc cgccctgcac cacgaccccg
      361 acttcgtgcc gcccttccgc ggtgacgcac gcagcaccaa ggccgccgac gagatgcccg
      421 tgtcgcggca gaacttcatc gagctctgcg acgagctgac ggtgcgcgac gaggaggcct
      481 tcgagtccct cttccgtcgc atcgggcaca gctacgactg gtcgatcacc taccgcacga
      541 tcgacgacca ctcccgcgcc accgcccagc aggcgttcct gcgcaacctg gcccgcggcg
      601 aggcctacca ggccgaggct ccggggctgt gggacgtcac gttccagacg gccgtcgccc
      661 aggccgagct cgaggcccgc gactaccccg ggcactacca ccgggtggcc ttccacggcc
      721 cccatggccc ggtgcacatc gagaccaccc gccccgagct catcccgagc tgtgtggccc
      781 tgatcgccca ccccgacgac gagcgctacc agggcctgtt cggcacgacg gtgacgactc
      841 ccctgttcgg tgtggaggtc cccgtgctcg cccacccggg cgccgagatg gacaagggtg
      901 ccggcatcgc gatgtgctgc acgttcggtg acctcaccga cgtcatctgg tggcgcgagc
      961 tgcagctgcc gacccgctcg gtgatcaccc gcagcggtcg cctccagggc gagacccctg
     1021 cctggctcga gggcacgccc ggtgaagccc tctacgccga gcagctcgcg ggcaagaccc
     1081 cgttcggggc acgcgccgcc ctcgtcgacg ccctgcgcga gtccggtgac ctcgacggcg
     1141 agcccaaggc cacccagcgc atggcgaact tctacgagcg cggtgagaag ccgctcgaga
     1201 tcgtcacctc gcgccagtgg tacatccgca acggcggccg cgacgccgag ctcaacgcgg
     1261 cgctgatcgc ccgcggggag cagatcgact tccaccccgc gttcatgcgc agccgctacg
     1321 ccaactgggt gagcgggctc aacggcgact ggctggtctc ccgccagcgc ttcttcggtg
     1381 tgccgatccc cgtctggtac cccctcgacg ccgacggcga gccggtctac gacaccccga
     1441 tcgtcccgga cgagagcgcc ctgccggtcg accccgcggc caacccgccg gccggctaca
     1501 ccgaggacca gcgcggcgtg cccggcggct tcatcggcga ccccgacgtg ctcgacacct
     1561 gggccacgtc ctcgctcagc ccgcagatcg ccggcgggtg gcgcaacgcc gacggcaccg
     1621 gcaccgaccc gctgttcgac aaggtcttcc cgatggacgt gcgcccccag ggccacgaca
     1681 tcatccgcac gtggctcttc tccacggtcg tgcgcgccca cttcgagcac ggcagcgtgc
     1741 cgtggaccga cgccgccatc agcggctgga tcctcgaccc cgaccgcaag aagatgagca
     1801 agtcccgcgg caacgtcgtc acccccgagg acctgctcaa ggagcacagc gcggatgccg
     1861 tgcgctactg ggcggccagc ggtcgcctgg gcacggatgc cgcctacgac accggccaga
     1921 tcaaggtcgg ccggcgcctc gcgatcaagg tgctcaacgc gagcaagttc gcgctgtcgt
     1981 tcgggtcggt cgagggtgac ctcgccgcgc aggtgaccga gccgatcgac ctgtccctgc
     2041 tcgccggcct gcgcgacgtc gtggccaagg ccaccgccgg tttcgaggcc tgggacttca
     2101 cccgcgccct cgaggtcacc gagcagtggt tctggtcgtt ctgcgacgac tacctcgagc
     2161 tcgtcaagga ccgcgcctac ggtgcccagg gtgacgccgg ggcggcatcg gcccgtgcga
     2221 cgctgcggat cagcctcgac gtggtgctgc ggctgttcgc gccggtcctg ccctacgtca
     2281 ccgaggaggt ctggtcctgg acccacgacg ggtcggtgca ccgcgcctcg tggccgacgg
     2341 tcgacgaggt gcccggcagc ggcgacccgg ccgtgctcgg gtcggtgggt gccgccctgg
     2401 ccgtcgtgcg caaggcgaag tcggaggcca aggtcggcat gcgcgccgag gtgccctcga
     2461 tgaccctcgt cgggccggat gccgtgcacg cccacgtgcg cagcgccgaa ggtgacctgc
     2521 gagcggccgg caagctcacc ggcatcctcg actacgccga aggcgatgag gtcgccgcgc
     2581 gcgacatcga gctcgtggcg gtccccaagc ccacggcctg acccacccga ccctgaccca
     2641 ccccgcccac atagttcccg acttcgtgcg acttgcgtgc ggtctttccg gccaaaagcc
     2701 cgtgaaaccg caccgaagtc gcacgaagtc gcgttcagat ggagtcaggc gggggctgcg
     2761 tccggggtga cggcgtaccc gcctcggatg accgggcgca gccccgtcgg gcgcaccgcg
     2821 aaccacggca cgtcccaccc gctgagcgcc agctgtgcgt cgacgacgac gtcacgccgc
     2881 aggtgagtga cccggccgct gccgggatcg tagacgtcga gcatgcggtc accgtccccc
     2941 gggaggatga gcacgacgtg tcggggcagc gtggcgttgc cgacgtacag cagcgccggt
     3001 tcaccctcgg cgacgacctc gacgagggtg tcgaacgcca ggacccggtc ggtccgggag
     3061 tcggcgcgca ggacgtcgat cgtgtactgc gtgccgcggc gagcagcccc gaactcgagc
     3121 tcgcgacggg ccccccacgg cggcgtcccg agttggcggg gccaggggac gttgagccga
     3181 ccggatccgg ggaacaggct gttggttcgc cgcatgacga tgcgttcgta ggcggcgaac
     3241 cgctcccccg gggtggcccc ctgcggagaa cccggcggat tgggaaaacc ggtgcgcacc
     3301 cacgaggcga agtgggggtc gacgtgcatg cgggccacag tgaggcaggc cgagccgcac
     3361 gtcacggggg actgctgcac ggggccggtg tctccggcgc gcagccgata gggcatcacc
     3421 actcccccat cgtgtcggcc ccggggtcag cgcgccagca ccgaccgtgg ggcgggcggg
     3481 tggggcgggt ccgccctcgt gctgctcgtc gtcaagctcg cgaacctcgt tcccgagggc
     3541 gggcggtcaa gctgattcgc gctcgatgag gtcgacgggc tggacgatgc cgcgaccgtc
     3601 cggggtgctc ggggcgggct cggactcgac gtcgtcgacc tcgtggccga gctggcgcag
     3661 cagcaaccgg gccatcagcc gtccctggtt ctcggtgcgc tggcggatgg tcgtcaggct
     3721 gggttcggcc atgctcgcca gctcgatgtc gtcgaacccg acgatcgcca cgtcatcggg
     3781 gacgcgcttg ccgagcttgc ggatcacgct gatggcaccg agcgccatga ggtcggacgc
     3841 gacgaagatg ccgtcgatgt ccggtgaccg ctcgaagagc cgggtcgccg cggcctcccc
     3901 cgaggccatg gtgaacaggc cgtgctcgac gaggtcttcc cggaagtcgg ggccgagccc
     3961 ctggcgaaag ccctcgagcc ggtcacaccc ggccgacatg tcggcggggc ccgcgatcgt
     4021 ggcgacccgg cggcatccgc gggcccgcag gtgctcggcc gccaggcgtc cgccggccac
     4081 gttgtcgttg tcgacgtacg ggacctcgac cgcggggtcg aggggtcgcc caccgatgac
     4141 gacggggatg ccggcctcga cgagcttctc ggccaggcgg tggcccccgt gctcggagat
     4201 gaggaggacg ccgtcggtgt gaccacccgc gaggtagcgc gacagcgatg tgatctgaag
     4261 atcggactgg gtcatcgaca gggacagctg caccccggcc cgggtgagct cctggctcgc
     4321 accccgcacg atctgcgcga agaacgggtc cccgaacacg cggtcgtcac cttcggctgc
     4381 gacgagcgtg acgctgtcgg tgcgccgcgt catcagggct cgcgcagcag cgttgggcac
     4441 gtagttcagg tcgtcgacgg cctgattgac gatctcgcgc agcgcggggt ccaccgtggt
     4501 cgagccgttg acgactcgtg agaccgtcgc ccgtgacacc cccgcgtagc gagccacctg
     4561 ttcgagcgtg ggccggcgcc ggcgagccgg agccgtcatg tcaggcggag cgcttgcggg
     4621 gagcgcgctt gcgggtggga tcgtcactgt gacgcacgat ggtcggcagg acgttcttgc
     4681 ggacgacctc actggtgacg acgaccttgg cgatctcgtc gtcgctcggc acgtcgaaca
     4741 tcgtcggcag caggacctct tccatgatcg cgcgcaggcc acgagcgccg gtgccgcgag
     4801 ccaacgcctg gtcggcgacg gccgcgacgg catcctccgt gaactccagg acgacgccgt
     4861 cgatctcgaa catcttctgg tactgcttga tgagggcgtt gcgcggctcg gtgaggatgc
     4921 gcacgagggc gtcgttgtcg agcggcgcca ccgtcgtgat gaccggcacg cgcccgatga
     4981 actcggggat caggccgaac ttcatgaggt cctcgggcat gacctcgtgg atgctctcgg
     5041 cgaggtcctt cggggtgtgc agctcggagc cgaagcccag gcccttcttg ccgttgcggg
     5101 actcgatgag cttctcgagg ccggcgaacg ccccgccgac gatgaacaac acgttggtcg
     5161 tgtcgatctg gatgaactcc tggtgggggt gcttgcgccc accctgcgg
//
LOCUS       DAOSOC010000171         5053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_16716_length_5053_cov_8.306523, whole genome shotgun sequence.
ACCESSION   DAOSOC010000171 DAOSOC010000000
VERSION     DAOSOC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16716_length_5053_cov_8.306523"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="PL014_11055"
     CDS             <1..1782
                     /locus_tag="PL014_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT57671.1"
                     /translation="DTETDNHFVPAVHLEGPNADLLAFLGSHSGVTATWAAGQPATVR
                     GDVMAGFSSRGPLGDFLKPDITAPGVQILAGHTPTPLTYNGGPEGELFQAIAGTSMSS
                     PHLAGASALVKAAHPTWTPGQIKSALMTSSTQDVLKEDGVTPADPFDRGAGSMRVDAA
                     VAAVATISETPENLYGIAAMDRVDLNLPSIQASPLPGAITTQRTLTNTTTRDQPFKAS
                     ATGAKGLKVSVSPSSFSVPAGGTRTITITIDGTAAPDGWAFGQVTLTSTNRKAPSLVL
                     PVTARVADSVVPMTHTCEPTDLARNQDAACTVTLTNNSAAPAPVKLSLAAPSRVDISS
                     VSAPATGTSRGFTWNGTLTPALPPTITSVTPGATPAGGYLPLSAFGIAPIAGMGDETI
                     VNYNVPPFTYGTETYTRVGVVSNGYVVIGGGTAQDVDYLNQPIPSTTRPNNIIAPFWT
                     DLNPGAGGAVRVGSLSDGTNDWLVIDYDKVPNWSSAAQQNSFQVWIGLNGTDDVTIAN
                     SVVTGGDGGFLSQGAENRDGTSGAAYSGGSNTAWVVNATGPTPGGSVQITYDASSSRA
                     GSYVLAPTATSPLVKGTISVPQTLRVK"
     gene            complement(1822..2562)
                     /locus_tag="PL014_11060"
     CDS             complement(1822..2562)
                     /locus_tag="PL014_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOT57672.1"
                     /translation="MRVLLDPRRPSHPGARVTAAELADLYAFPHRRWVRSNFVSTLDG
                     SAVGADGLSGSINTPADNRVFALQRSLCQAVLVGAGTVRAEGYERLEPTRARPVPPAL
                     VVVSASGRVPEGLRTPTAGRGAGLLVTCESAGERALERARRVLGPDAVLVAGGAHVDL
                     ARALDALAERGLGQVLCEGGPSLFASALSAGVVDEVALSLAPTVVGGDGTRVTSGPAL
                     GDADGIHLTPRLLLEEAGTMLGLWRVRT"
     gene            2646..3608
                     /locus_tag="PL014_11065"
     CDS             2646..3608
                     /locus_tag="PL014_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOT57673.1"
                     /translation="MGKGAKKSKKSVEKAELTGKAARKAEKKRQKAAKARQDDAVQAV
                     AWSHVLRVGPGFDLAEFDPASTPGFTGDRAAAEAVMAELQERTSDLQERLYAESRGGG
                     QRSVLLVIQGMDTSGKGGIMRHVVGAVDPQGVEITSFKAPTAEEKRHPFLWRIRRALP
                     SAGDIGVFDRSHYEDVLIVRVHDLVPRSTWSRRYAQINDFEAGVVESGTTIIKVMLHI
                     SSDEQKARLTERLAREDKHWKYNPGDVDERMHWVDYMQAYQAALEKCSTAAAPWFVVP
                     ADNKWYARLAVTNLLLEHLEAMDPQWPAADFDVEAEKVRLALSP"
     gene            3696..4847
                     /locus_tag="PL014_11070"
     CDS             3696..4847
                     /locus_tag="PL014_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOT57674.1"
                     /translation="MTDAKRRLRRLLAQVPGLLPLARLVVVTTRICLRYRVTGLASEA
                     GFFALLSLPPLVLGLFGGLGYLGTALGPVAVEQVRDAINDYASRFLTEDIINSTLGPT
                     INDVFTDGRFDLISLGFVLSLWSGSRALNVFVDTISIMYGQSGVRGIVQTRALSFSLY
                     LLALVLGIVTIPLVLLGPTLLGEILPDPWDALTALYWPLVTLLVVGGLTSLYHVSTPR
                     RSPWLRDAPGAVVTLLIWILASFVVRGTIAASLGGTSIYGPLSAPIVLLIWLYALAIA
                     VLIGAAANAAIRELWPVEENRSLHERFGAWMGEHWRRRSAPRTTVGWDDPFDGYGRGL
                     ALDEDDLGLSGLREAARGPMTPISRASRGDGADAAGEAVGAGRRGDSQA"
BASE COUNT          790 a         1824 c         1724 g          715 t
ORIGIN      
        1 gacaccgaga ccgacaacca cttcgtcccg gcggtccacc tcgagggacc gaacgccgat
       61 ctgctcgcct tcctcgggtc ccacagcggg gtcaccgcca cgtgggcggc cggccaaccg
      121 gcgacggtgc ggggcgacgt catggcgggc ttcagctcgc gcgggccgct gggtgacttc
      181 ctcaagcctg acatcaccgc ccccggcgtg cagatcctcg ctgggcacac cccgacgccg
      241 ctcacctaca acggcgggcc cgagggtgag ctgttccagg ccatcgccgg cacctcgatg
      301 tcgagcccgc acctggccgg tgcctcggca ctggtcaagg cggcgcaccc gacctggacg
      361 cccggccaga tcaagtcggc cctcatgacg tcctcgacgc aggatgtgct caaggaggac
      421 ggcgtgaccc cggcggaccc gttcgaccgc ggtgccggct cgatgcgcgt cgatgcggcc
      481 gtcgcggccg tcgccacgat cagcgagacc cccgagaacc tctacgggat cgctgccatg
      541 gaccgggtcg acctcaacct gccgagcatc caggccagcc cactgcccgg ggccatcacc
      601 acgcagcgga ccctcacgaa cacgaccacg cgcgaccaac cgttcaaggc cagcgccacg
      661 ggtgccaagg gcctgaaggt cagcgtgtcg ccgagttcgt tctccgtgcc ggccggtggg
      721 acgcgcacga tcacgatcac gatcgacggc acggccgcac ccgacggttg ggccttcggc
      781 caggtgacgc tcacctccac gaaccgcaag gcgccgagcc tcgtcctgcc ggtgaccgca
      841 cgcgtggccg acagcgtcgt cccgatgacg cacacctgcg agccgaccga cctggcgcgc
      901 aaccaggacg ccgcgtgcac cgtgaccctc acgaacaaca gcgccgcccc ggcaccggtc
      961 aagctctcgc tcgccgcacc gagccgggtg gacatcagct cggtcagcgc cccggccacc
     1021 ggcacctcga gagggttcac ctggaacggc acgctcacac ccgccctgcc gccgaccatc
     1081 acctcggtca ccccgggcgc cactccggcc ggcggctacc tgccgctgag cgcgttcggc
     1141 atcgccccga tcgccggcat gggcgacgag acgatcgtga actacaacgt cccaccgttc
     1201 acctacggca cggagacgta cacccgcgtc ggcgtcgtct ccaacgggta cgtcgtcatc
     1261 ggcggcggaa cggcccagga cgtcgactac ctcaaccagc cgatcccctc gaccacgcgg
     1321 ccgaacaaca tcatcgcgcc gttctggacg gacctgaacc ccggtgccgg tggcgcggtg
     1381 cgtgtcggct ccctcagcga cgggaccaac gactggctcg tcatcgacta cgacaaggtg
     1441 ccgaactgga gctccgccgc ccagcagaac tccttccagg tctggatcgg gctcaacggc
     1501 accgacgacg tgacgatcgc caactcggtg gtgaccggcg gcgacggagg cttcctcagc
     1561 cagggcgcgg agaaccgtga cggcacgagt ggcgccgcgt actccggcgg ctcgaacacg
     1621 gcctgggtcg tcaacgcaac aggcccgacc ccgggaggct cggttcagat cacctatgac
     1681 gcgtccagtt cacgggcggg ctcctacgtc ctggctccga cggcgacgtc gccactggtc
     1741 aagggcacga tcagcgtccc gcagacgctt cgagtcaagt gaccgggtgg gggtgggcgc
     1801 cttgcccacc cccaccgcgg gtcaggtccg aacccgccag agtccgagca tcgtgcccgc
     1861 ctcctccagc aggaggcggg gggtgaggtg gatgccgtcc gcgtcgccga gcgctggccc
     1921 gctcgtcacc cgcgtgccgt cccctccgac gacggtgggc gcgagggaca gggcgacctc
     1981 gtcgacgacc ccggcggaca gggccgaggc gaacaggctg gggccgccct cgcacaggac
     2041 ctgaccgagc ccgcgctcgg cgagggcgtc caatgcacgc gcgaggtcga cgtgggcacc
     2101 tccggcgacg agcacggcat ccggaccgag cacgcggcga gcacgctcca gggcgcgctc
     2161 accagctgac tcacaggtga cgagcagccc cgcgccacgc ccggcggtcg gggtgcgcaa
     2221 cccctcggga acacgaccgg aggcgctgac gacgaccagc gcggggggca ccggtcgtgc
     2281 ccgcgtgggt tcgagccgct cgtacccctc ggcccgcacg gtcccggcgc cgacgagcac
     2341 cgcctggcac agcgagcgtt ggagggcgaa cacccggttg tcggcggggg tgttgatgct
     2401 tccgctcaga ccgtcggccc cgaccgctga accgtcgagg gtgctgacga agttcgagcg
     2461 cacccagcgg cggtgaggaa aggcatagag gtcggccagt tcagccgccg tgacgcgtgc
     2521 gcccgggtgc gacggtcggc ggggatcgag gaggacgcgc atggcgtgag tgtcgcaggg
     2581 aacagggctg ggggaccccg gatgttccgg ggcatccgag gcgtcgggtc tgcaaggatg
     2641 cggcgatggg caagggtgcg aagaagagca agaagtccgt cgagaaggcc gaactgaccg
     2701 gcaaggccgc gaggaaggcc gagaagaagc gccagaaggc cgccaaggca cgccaggacg
     2761 atgcggtgca ggcggtggcc tggtcgcacg tcctgcgggt ggggcccggt ttcgacctcg
     2821 ccgagttcga ccctgcctcg acgccggggt tcaccggcga cagggcggcc gctgaggccg
     2881 tcatggccga gctccaggaa cgcaccagcg acctccagga gcggctctac gccgaatcga
     2941 gggggggagg tcagcgctcg gtgctgctcg tcatccaggg catggacacc tccggcaagg
     3001 gcggcatcat gcgccacgtc gtcggtgcgg tcgacccgca aggagtggag atcacgtcgt
     3061 tcaaggcgcc gaccgccgag gagaagcgcc acccattcct ctggcggatc cggcgtgcac
     3121 tgccgtcagc cggtgacatc ggcgtcttcg accgcagcca ctacgaggac gtcctcatcg
     3181 tgcgggtgca cgacctggtg ccacggtcga cctggtcgcg ccgctacgcc cagatcaacg
     3241 acttcgaggc gggagtcgtc gaatcgggga ccacgatcat caaggtcatg ctgcacatct
     3301 ccagcgacga gcagaaggcg cgcctcaccg aacgactcgc ccgcgaggac aagcactgga
     3361 agtacaaccc cggcgacgtc gacgagcgca tgcactgggt ggactacatg caggcctacc
     3421 aggcagccct ggagaagtgc tcgaccgctg ccgcgccgtg gttcgtcgtg ccggcggaca
     3481 acaagtggta cgcccggctc gccgtgacga atctgctgct ggaacacctc gaggcgatgg
     3541 acccgcagtg gcccgcagcc gacttcgacg tcgaggcgga gaaggtccgc ctggcgctct
     3601 cgccctgagg gtgatcgcga ccagaacgct caaggcaacc ctcgtgcagc cctgatgtcc
     3661 gcggttctcg cgggcgcggc ggttaccgtt gccccatgac cgatgcgaaa cggcgcctgc
     3721 gccgcctgct cgcacaggtc ccgggcctgc tgcccctggc ccggctggtg gtcgtgacca
     3781 cgcgcatctg cctgcgctac cgggtcaccg gcctggcgtc cgaggcgggg ttcttcgccc
     3841 tgctcagcct gccgccgctg gtcctggggc tcttcggcgg cctcggctac ctcggcaccg
     3901 ctctcgggcc ggtggccgtg gagcaggtgc gcgatgcgat caacgactac gcgtcgcgct
     3961 tcctcaccga ggacatcatc aactccacgc tcggcccgac gatcaacgac gtcttcaccg
     4021 acggacgctt cgacctcatc tccctcggct tcgtcctgtc gctgtggtcc ggatcccggg
     4081 cgctcaacgt cttcgtcgac acgatctcga tcatgtacgg ccagtcgggg gtgcgcggca
     4141 tcgtccagac gagggcgctg tcgttctcgc tctacctcct ggcgctggtc ctcggcatcg
     4201 tgacgatccc cctcgtgctg ctcggcccca ccctgctcgg cgagatcctt cccgacccgt
     4261 gggatgccct cacggccctc tactggccgc tcgtcaccct gctcgtcgtc ggggggttga
     4321 ccagcctgta ccacgtgtcg actccgagac gctcgccctg gcttcgtgac gctcccggcg
     4381 ccgtcgtcac cctgttgatc tggatcctcg cctccttcgt cgtgcgcggg accatcgcgg
     4441 catccctggg tggcacgtcg atctacggcc cactgtccgc tcccatcgtg ctgctcatct
     4501 ggctctacgc actggccatc gccgtgctga tcggtgccgc cgcgaatgcc gccatccgtg
     4561 agctgtggcc ggtggaggag aaccgcagcc tccacgagcg cttcggtgcc tggatgggtg
     4621 agcactggcg ccgccggtcg gctccgcgca ccaccgtggg ctgggacgac cccttcgacg
     4681 gctacggccg cgggctcgcg ctcgacgagg acgacctcgg cctgtccggg ctgcgggagg
     4741 ccgcccgcgg cccgatgacg ccgatctccc gggcatcacg cggtgatggt gcggatgccg
     4801 cgggggaggc agtcggtgcc ggtcgccgcg gcgactcgca ggcctgagcg ctggcccgcc
     4861 gtggtgggga ggcactcggc gagtggtcgg gaagcagtcg ggggaggggt catgggggcc
     4921 cggtttggcg atcccctcga ctgtcagata atgtctgcga gcacgccaag cgcggttcgc
     4981 aagagccgca cggacgcgcg cggatgtagc tcagttggta gagcgcaacc ttgccaaggt
     5041 tgaggtcgcc ggt
//
LOCUS       DAOSOC010000172         5036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_16782_length_5036_cov_8.742823, whole genome shotgun sequence.
ACCESSION   DAOSOC010000172 DAOSOC010000000
VERSION     DAOSOC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16782_length_5036_cov_8.742823"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1655)
                     /locus_tag="PL014_11075"
     CDS             complement(189..1655)
                     /locus_tag="PL014_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT57675.1"
                     /translation="MSIAPSTQSHSFHRRPRLRAVPDVALLAPSTGSDAVARLAHPAH
                     RGATGLDHAGLPPVVRAGLVPTLSGDWVDYANLDHAASTPAFERVASAVESATRTYSS
                     VHRGTGWLSRVTSAHYEAARDEVARFVGAREDDVVVFTRGTTDSVNLLARALPRGTTV
                     IVFNSEHHATLLPWSKRGTVRLPVPGSARDAEVLLESALRDLPAGTARRPRQALVVLT
                     AASNVTGEFWPVESLTAIARRHGARVLLDAAQSAAHRPLDLAALDVDWVAFSGHKLHA
                     PFGTGVLAGRSDWLDAAAPYLAGGGATAQVTPESTRWATGAARHEAGSPNVLGAVALA
                     AACATIREHRAAIDAHEAALTTALLDGLRAIDGVQTYSLFGEDTARGPVVTFTVDGLD
                     SQLVAAALSAEHGIGVRAGKFCAHILVDALLDEGSDEHDSAVRVSAGLATTPEHVTRL
                     VAAVTALAANGPGAEYELTEQGWAPVDDTRELDAPLPW"
     regulatory      complement(1699..1814)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            complement(1911..4841)
                     /gene="leuS"
                     /locus_tag="PL014_11080"
     CDS             complement(1911..4841)
                     /gene="leuS"
                     /locus_tag="PL014_11080"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT57676.1"
                     /translation="MSETPYRYTAELAGQIEVAWQDRWEQRGTYHAPNPAGPWADPEG
                     VAAHSGGHLLVLDMFPYPSGAGLHVGHPLGYIATDVFARFNRMLGKNVLHCLGYDAFG
                     LPAEQYAVQTGQHPRKTTEDNIVIMARQLRRLGLGHDDRRAIQTIDPDYYRWTQWIFT
                     QIYNAWYDADAVRPDGGTGRARPIAELEQEYRDGTRPLPGGEDRAWDTLSATERAGIL
                     DASRLAYTSEAPVNWCPGLGTVLSNEEVTNEGRSERGNFPVFKRNLRQWMMRITAYAD
                     RLADDLDRVEWPEKVKIMQRNWIGRSHGAKVTFPIARPSGDQLVDLGTADAGIEVFTT
                     RPDTLFGATFMVLAPEHPLVDALVPAGGWPEGTKPAWTGGADTPAQAVAAYRLAASRK
                     NDIERQSEGKDKTGVFTGGWATNPVTGTQVPVFIADYVLMGYGTGAIMAVPAQDERDW
                     DFATAFDLPIVRTVQPSEGHDENTAFTGDGPAINSSNDDVDLNGLGVAQAKERITAYL
                     AERGLGEATVTYKLRDWLFSRQRYWGEPFPIVFDEDGVAHALPDEMLPVELPEVPDYS
                     PKTYDAEDASSEPEPPLSRVPDWVEVDLDLGDGRGTRRFRRETNTMPNWAGSCWYYLR
                     YLDPANEGVLVDPENEAYWMGPRSEPVAGAPAGTRDPGGVDLYVGGVEHAVLHLLYAR
                     FWHKVLHDLGHLSSDEPFRTYFSQGYIQAAAYRDDRGQPVEAKEVQEGPDGTFTWQGQ
                     PVTREFGKIGKSLKNMVSPDEMYASFGADTFRLYEMGLGPLEQSKPWDTRAVVGSQRF
                     LQRLWRNVVDEESGEVVVVDTPIDDATNRVLHRTIDAVRADYAGLGFNTAIARLTELN
                     NALTKLPGGVPRAAAEQIVLMVAPLAPHIAEELWARLGHTESLVHEAFPVADPALLVE
                     DTVTCVVQVRGKVRDRFEVPADISEDDLRELALASEKVQAAAPDGIRTVIVRAPKLVN
                     VVPA"
BASE COUNT          675 a         1729 c         1879 g          753 t
ORIGIN      
        1 tcgaacctgt gagcccgggt accagtgctg tcaggagtcc ttgcgcccga caaggaagtc
       61 cttgcgcggc gaaaggtctc ctgacagcac cccctatgcc cctctctcag cctcgagcag
      121 ggtcgggtcg ccagccccgc gatgggccac cggagcacca agtcgtcggc cgccgaagcg
      181 ccgatcgctc accagggcag cggcgcgtcc agctcgcggg tgtcatccac cggcgcccag
      241 ccctgctcgg tcagctcgta ctcggcgccc ggcccgttgg cggccaacgc cgtcacggcc
      301 gccaccagcc gggtcacgtg ctccggggtg gtcgccagcc cggcgctgac ccgcacagcg
      361 ctgtcgtgct cgtccgaccc ctcgtcgagc agcgcgtcga cgaggatgtg cgcgcagaac
      421 ttgccggccc gcaccccgat gccgtgctct gccgacagcg ctgccgccac gagctggctg
      481 tcgagcccgt cgacggtgaa cgtcacgacc ggcccgcgcg cggtgtcctc accgaacagc
      541 gagtaggtct gcaccccgtc gatggcgcgc aacccatcga gcagggccgt ggtgagagca
      601 gcctcgtggg cgtcgatggc ggcccggtgc tcgcggatcg tcgcgcacgc agccgcgagc
      661 gccacggcac cgagcacgtt cgggctcccg gcctcgtggc gggcagcacc cgtcgcccag
      721 cgggtcgact cgggcgtgac ctgcgcggtc gcgccgccac ccgccaggta gggcgctgcg
      781 gcatccagcc agtcgctacg cccggcgagc accccggtgc cgaacggggc gtgcagcttg
      841 tgacccgaga aggcgaccca gtccacgtcg agcgccgcca ggtcgagcgg gcggtgggcg
      901 gccgactgcg cggcgtcgag cagcacgcgc gcgccgtggc gacgggcgat ggccgtcagg
      961 gactcgaccg gccagaactc gccggtgacg ttgctcgcag ccgtcagcac gaccagggct
     1021 tggcgcgggc gccgagcggt gccggccggc aggtcacgaa gggcgctctc cagcaggacc
     1081 tcggcatcgc gggcgctgcc cggcacgggc aggcgcaccg tgccccgctt ggaccacggc
     1141 agcagcgtcg cgtggtgctc ggagttgaac acgatgacgg tcgtgccccg cggcagggcc
     1201 cgcgcgagca ggttcaccga gtcggtggtg ccgcgggtga acacgacgac gtcgtcctca
     1261 cgggcaccga cgaagcgggc cacctcgtcg cgagccgcct cgtagtgggc gctggtcacc
     1321 cgcgagagcc accccgtgcc gcggtggacg ctggagtacg tgcgggtcgc cgactccacg
     1381 gcgctcgcaa cgcgctcgaa ggccggggtg gatgccgcgt ggtcgaggtt cgcgtagtcg
     1441 acccagtcac cggacagggt cggcacgagg ccggcgcgaa ccaccggggg caggccggcg
     1501 tggtcgaggc cggtggcccc ccggtgcgcc gggtgcgcga ggcgcgccac ggcatccgac
     1561 ccggtgctgg gggcgagcag ggcgacatca gggacggccc gcagcctcgg gcggcggtgg
     1621 aaggagtggg actgggtgct cggggcgatc gacacgatgg aggtctccgt cgggactgtg
     1681 gacctcgggt gaccccgtga tccgcgcttg ccggcgccgg tttcgacgtc gaccaggtcg
     1741 tcacccggag caccccaccg cggaaggagg gttgctgacc aacgagccgg ggcttgtcgc
     1801 tggcactcat gacctacgtc ggaaatcgta ggagcggatg ccgcgccacg gcatccgcgg
     1861 tccacgacgc gagacacccc cggggcgcgg tccccggggg tcgcctcagg tcaggcaggg
     1921 acgacgttga cgagcttggg tgcccggacg atgacggtgc ggatgccgtc cggcgccgct
     1981 gcctggacct tctcgctcgc cagcgccagc tctcgcaggt cgtcctcgct gatgtccgcg
     2041 ggcacctcga agcggtcgcg gaccttgccg cggacctgca cgacgcaggt gacggtgtcc
     2101 tcgacgagca gggccgggtc ggccaccggg aacgcctcgt ggaccagcga ctcggtgtgt
     2161 ccgagacgcg cccacagctc ttcggcgatg tgcggcgcga ggggcgcgac catgagcacg
     2221 atctgctcgg cggccgcgcg gggcactccc ccgggcagct tggtcagggc gttgttgagc
     2281 tcggtgagcc gggcgatggc cgtgttgaag cccagccccg cgtagtcggc acgcacggca
     2341 tcgatggtgc ggtgcagcac ccggttcgtc gcgtcgtcga tcggggtgtc gacgacgacg
     2401 acctcgccgc tctcctcgtc gacgacgttg cgccacagcc gctggaggaa gcgctggctg
     2461 ccgacgacgg cccgggtgtc ccagggcttg ctctgctcga gcgggcccag tcccatctcg
     2521 tagaggcgga aggtgtcggc accgaacgag gcgtacatct cgtcggggct gaccatgttc
     2581 ttcagcgact tgccgatctt gccgaactcg cgggtgacgg gctggccctg ccaggtgaag
     2641 gtgccgtcgg ggccctcctg cacctccttg gcctccaccg gctggccgcg gtcgtcgcgg
     2701 taggccgcgg cctggatgta gccctggctg aagtaggtgc ggaacggctc gtcactgctc
     2761 aggtggccca ggtcgtgcag caccttgtgc cagaaccggg catagagcag gtgcagcacg
     2821 gcgtgctcga caccgccgac gtacaggtcg acgccacccg ggtcccgggt accggcgggg
     2881 gcgcctgcca cgggctcgga gcgcgggccc atccagtacg cctcgttctc ggggtcgacg
     2941 aggacgccct cgttcgccgg gtcgaggtag cgcaggtagt accagcacga gcctgcccag
     3001 ttcggcatgg tgttggtctc gcggcggaag cgccgggtgc ctcggccgtc accgaggtcg
     3061 aggtcgacct ccacccagtc ggggacacgc gacagcggcg gctcgggctc gctgctggcg
     3121 tcctcggcgt cgtaggtctt cgggctgtag tcgggcacct cgggcagctc gacgggcagc
     3181 atctcgtcgg gcagcgcgtg ggcgacaccg tcctcgtcga agacgatcgg gaagggctca
     3241 ccccagtagc gctgccggct gaacagccag tcgcgcagct tgtaggtgac ggtcgcttcg
     3301 ccgaggcccc gctcggccag gtaggcggtg atccgctcct tggcctgcgc gacccccagc
     3361 ccgttgaggt cgacgtcgtc attgctgctg ttgatcgccg ggccgtcacc ggtgaaggcg
     3421 gtgttctcgt cgtgcccctc ggacggctgc acggtgcgga cgatcggcag gtcgaacgcg
     3481 gtcgcgaagt cccagtcgcg ctcgtcctgt gcgggcacgg ccatgatcgc gccggtgccg
     3541 tagcccatga gcacgtagtc ggcgatgaac accggaacct gcgtgccggt caccgggttg
     3601 gtggcccagc caccggtgaa gacaccggtc ttgtccttgc cctccgactg gcgctcgatg
     3661 tcgttcttgc gcgaggccgc gagtcggtag gcggcgacgg cctgcgccgg ggtgtcggcg
     3721 ccaccggtcc acgcgggctt ggtgccctcg ggccagccgc cggccggcac gagcgcgtcg
     3781 acgagcgggt gctcgggggc cagcaccatg aacgtggcgc cgaacagcgt gtcgggacgg
     3841 gtcgtgaaga cctcgatccc ggcatccgcc gtcccgagat cgaccaactg gtcaccggag
     3901 ggacgggcga tcgggaaggt gaccttcgca ccgtggctgc gcccgatcca gttgcgctgc
     3961 atgatcttga ccttctcggg ccactcgacc cggtcgaggt cgtcggccag ccggtcggcg
     4021 tacgcggtga tccgcatcat ccactggcgc aggttgcgct tgaacaccgg gaagttgccc
     4081 cgctcggagc ggccctcgtt ggtgacctcc tcgttggaga gcactgtgcc caggccaggg
     4141 caccagttga ccggcgcctc gctcgtgtac gccaggcgcg aggcatcgag gatgccggcg
     4201 cgctcggtcg cgctcaacgt gtcccacgcg cggtcctcac caccgggcag cgggcgcgtt
     4261 ccgtcgcggt actcctgctc gagttccgcg atggggcggg cgcggccggt cccgccgtcg
     4321 gggcgcacgg catccgcgtc gtaccaggcg ttgtagatct gggtgaagat ccactgtgtc
     4381 cagcggtagt agtcggggtc gatggtctgg atggcgcggc ggtcgtcgtg gcccaggccc
     4441 agtcggcgca gctggcgcgc catgatgacg atgttgtcct cggtggtctt gcgcgggtgc
     4501 tggccggtct ggacggcgta ctgctcggcc ggcagcccga acgcgtcgta ccccaggcag
     4561 tgcaggacgt tcttgccgag catgcggttg aagcgcgcga agacgtcggt ggcgatgtag
     4621 ccgagcgggt gcccgacgtg caggccggca ccgctcgggt aggggaacat gtcgaggacg
     4681 agcagatgcc cgccggagtg cgcggcgacc ccctcggggt ctgcccaggg gccggccggg
     4741 ttcggggcgt ggtaggtgcc tcgctgctcc cagcggtcct gccaggccac ctcgatctgc
     4801 ccggccagct cggcggtgta gcggtacggg gtctcgctca tctcgtcctc gttcgtcgtg
     4861 tcgtcgtgcg cggatgctcg gtggtgcgct catgaaaaaa cccctcgctc aggaggggtc
     4921 gccgcgcggc tggggtggcc gtcgcggcta ggtaagcagg agcaccgtgg tcacggggtc
     4981 agggtatcgc agcaggtggg tcaggccagc aggccggcga cgatcagggc ctgcgc
//
LOCUS       DAOSOC010000173         5025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_16827_length_5025_cov_10.044064, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000173 DAOSOC010000000
VERSION     DAOSOC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16827_length_5025_cov_10.044064"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PL014_11085"
     CDS             <1..639
                     /locus_tag="PL014_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOT57677.1"
                     /translation="IDGGRIWVDGDLMGYEDRGGVLHHLTDKQIAGQRREIGMCFQRF
                     NLFPHKTVLENIIEAPTQVKGVSKAEAKARAVELLEQVGLSDKPAAYPSQLSGGQQQR
                     VAIARALAMDPKLMLFDEPTSALDPELVGEVLKVMRDLAEGGMTMIVVTHEMGFAREV
                     ADRVVFMDAGVVVEQGPPSEVIGNPQHARTRAFLSRMRQEEAAHAAAQDPIA"
     gene            660..980
                     /locus_tag="PL014_11090"
     CDS             660..980
                     /locus_tag="PL014_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOT57678.1"
                     /translation="MPLPALVRVRGVSMLPTHREGDVLLVVRGRHPRPGQVAVVRLPP
                     DASGTPRPLSVKRVTGPDPHDPTRWWIDSDNAAQGLTSFDVGSLATDDIVAVVVGRLW
                     RPLR"
     gene            1025..1420
                     /gene="sodN"
                     /locus_tag="PL014_11095"
     CDS             1025..1420
                     /gene="sodN"
                     /locus_tag="PL014_11095"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HOT57679.1"
                     /translation="MLSLFRITDVSAHCDLPCGVYDPAQARIEAESIKAIIAKVADND
                     DPDFRTRAIVIKEQRSELVKHHLWVLWTDYFKPPHFEKYPELHTLVNEATKLAGASGT
                     KGELDAAKADELLAKIDEIAKIFWETKKA"
     gene            complement(1501..2661)
                     /gene="manA"
                     /locus_tag="PL014_11100"
     CDS             complement(1501..2661)
                     /gene="manA"
                     /locus_tag="PL014_11100"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOT57680.1"
                     /translation="MDRLTPVVFSDAWGSRVSIASLQGRDIPSPEPESELWMGAHEGG
                     ASGTDRPGNPDLAAVIAADAVQELGADCIDRFGPRLPFLLKVLAPGRAISIQAHPTAE
                     QACAERARTRDAVYVDDWAKPELLLALAPFEVFVGMRSFVEVVELARRLKVNRFTTIV
                     EQASSAADPVHEALALVLATPAEEVDEFARSIVAACVRLEASGDDLGHAAAAIVGVAE
                     EHPDDIGLVVLLLMHYRVLEPGEYVDVAAGVLHSYVRGLGIEVLANSDNVVRAGLTSK
                     EVNVAELLRIVDPRAGGIAGRGRVVEPGLEVFDSMSDRFVLYRVTPGRVLPGGRGPRI
                     AFCLRGTVTLRSGSRVLDLRDAESAFLPAGEGTVALEGSGEVYVASVPPPSS"
     gene            complement(2738..4069)
                     /locus_tag="PL014_11105"
     CDS             complement(2738..4069)
                     /locus_tag="PL014_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HOT57681.1"
                     /translation="MSVPRPLLERPRAAALLALGILTAAAALTTAFGSAGWASGLHYR
                     TIVVFVVALVAGELVRLRMPSRREAAPLASASALALAFVGDIAGEPTFDVPSGVVVLV
                     VAAGLVLAAVVRLAFGRAVRVDQMAARLVGVGVAAWLARAASPWGLPLWDLDVGEQVP
                     LTVVAAGFLVIAAIGLTVEIGLSSAVRSERQRTAWSSALRDEAGEVAPLTYAVITTGP
                     MVALMAPQLGLLALPVALFPLAITYVAVDRASRNREIYRQTIAILSGLTEAGGYTPSG
                     HAERVAVSSVRMGRVLGLSGRELRDVEYAALLHDLGQISLTEPIPGGATVLAAPSDQL
                     EISSEGARIIRRASGLENIASIVESVPTPYRRVRELGEDVALGSRIIKVANAYDDLTQ
                     KDSGASAVDAALERIQLGLGYEYDPDVVTALAVVTSATAPGRSGPGHRDPR"
     gene            complement(4066..>5025)
                     /locus_tag="PL014_11110"
     CDS             complement(4066..>5025)
                     /locus_tag="PL014_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT57682.1"
                     /translation="QPWRARLFNTGMFALLGVFGGVVYRAAGGVVGGAGLTGPWEIAR
                     AVGLPILVADVAQALANLLLIAMVVRLAQGVPMRTQIWRLLVTSGPNQLGYGVIAFIL
                     VVLWEPVGLGPFGVVLVLPPLLVARWAFNQYSDEVKGHERALGVLVGAVESKAPHLVG
                     HSARVAQLSVHMAESLGLRPAVVADIRAAGMLHDLGLTTLPTATVRRESVGASVALGD
                     YPQRGARLVQEVGFLAGSVDAVAHHREAVGSGAEGAALGLPALVVGLADEYDLLTEVG
                     SPDGRVLTHDEAMAALRARSPDDHGLLPTLEQALVRRANVAGA"
BASE COUNT          843 a         1819 c         1710 g          653 t
ORIGIN      
        1 atcgacggcg gccgcatctg ggtcgacggc gacctcatgg gctacgagga ccgcggcggg
       61 gtgctgcacc acctcaccga caagcagatc gctggacagc gccgcgagat cgggatgtgt
      121 ttccagcggt tcaacctgtt cccgcacaag acggtgctcg agaacatcat cgaggcgccc
      181 acccaggtca agggcgtcag caaggccgag gcgaaggctc gtgccgtgga actgctcgag
      241 caggtggggc tgtccgacaa gccggccgcc tacccgtcgc agctgtccgg cgggcagcag
      301 cagcgagtgg cgatcgcgcg ggcgctcgcg atggacccca agctcatgct cttcgacgag
      361 ccgacgagcg cgctcgaccc cgaactcgtc ggtgaggtgc tcaaggtcat gcgtgacctg
      421 gccgaaggcg gtatgacgat gatcgtcgtc acccacgaga tgggcttcgc ccgcgaggtc
      481 gccgatcgcg tcgtcttcat ggacgccggc gtcgtcgtcg aacagggccc cccgtcggag
      541 gtcatcggca acccccagca cgcccgcacc cgggccttcc tgtcccgcat gcgccaggag
      601 gaggccgcac acgccgccgc ccaggacccc atcgcatagc ggtgagaccc cggtcgcgga
      661 tgccgctgcc cgccctcgtg cgggtacgcg gcgtctcgat gctgcccacc caccgcgaag
      721 gtgacgtcct gctcgtggtg cgtgggcggc atccgcgccc cggccaggtc gccgtcgtgc
      781 gccttccccc ggacgcctcg ggcaccccga ggccgctgtc ggtcaaacgg gtcaccggcc
      841 ccgatccgca cgatccgacc cggtggtgga tcgactccga caacgccgcc caggggctga
      901 cgtccttcga cgtcggttcg ctcgcgaccg acgacatcgt tgccgtcgtc gtcggtcgac
      961 tctggcggcc gctgaggtag gttgacgggg accgcgagat ccccacatct tcaggagaga
     1021 gaccatgctg tcgctgttcc gcatcaccga tgtctccgcc cactgcgacc tgccctgtgg
     1081 cgtgtacgac ccggctcagg cacgcatcga ggccgagtcg atcaaggcga tcatcgccaa
     1141 ggtcgccgac aacgacgacc ccgacttccg cacccgtgcg atcgtcatca aggagcagcg
     1201 ctccgaactc gtcaagcacc acctctgggt gctgtggacc gactacttca agcccccgca
     1261 cttcgagaag taccccgagc tgcacaccct ggtcaacgag gccacgaagc ttgcgggcgc
     1321 gtccggcacc aagggcgagc tcgacgcggc gaaggccgac gagctgctcg ccaagatcga
     1381 cgagatcgcc aagatcttct gggagaccaa gaaggcctga cccggccttc ctgacctacc
     1441 tgtcggcagc cgaccgacat gccgcttcgc ggtgtgtcgg tcggcttgct gcatgaaggg
     1501 tcaggacgac ggtggcggaa cgctcgccac gtagacctcg ccggatccct cgagagcgac
     1561 cgtcccctca cccgcaggca ggaacgcgga ctcggcgtca cgcaggtcga gcacccggga
     1621 gcccgagcga agggtgaccg tgccccgcag gcagaacgcg atgcgaggcc ccctgccacc
     1681 gggcaggacg cgcccgggcg tcacccggta gagcacgaac cggtccgaca tgctgtcaaa
     1741 gacctcgagg cccggctcca ccacgcgtcc acgaccggcg atgccacccg cgcgcgggtc
     1801 gacgatccgc aggagctccg cgacgttgac ctccttgctg gtcagtccgg cgcgcaccac
     1861 gttgtcggag ttggcgagca cctcgatccc gagccctcgc acgtacgagt gcagcactcc
     1921 tgccgcgacg tcgacgtact cccccggctc gaggacgcgg tagtgcatga gcagcaggac
     1981 gacgagcccg atgtcgtcgg ggtgctcctc ggccaccccg acgatggcgg cggccgcgtg
     2041 gccgaggtca tctcccgacg cctcgagccg gacacaggcg gccacgatcg atcgtgcgaa
     2101 ctcgtcgacc tcctccgccg gggtcgcgag cacgagtgcc agcgcctcgt gcaccgggtc
     2161 cgccgccgac gacgcctgct cgacgatcgt cgtgaaccgg ttgaccttca ggcgccgagc
     2221 cagctcgacg acctcgacga aactgcgcat gccgacgaac acctcgaagg gggccagcgc
     2281 cagcagcagc tcgggtttgg cccagtcgtc gacgtagacg gcatcgcggg tccgggcgcg
     2341 ctccgcgcac gcctgctcgg ccgtggggtg tgcctggatc gagatggccc gccccggggc
     2401 cagcaccttg agcaggaaag ggagtcgagg gccgaaccga tcgatgcagt cagcccccag
     2461 ctcctggacg gcatcggcgg cgatcacggc ggcgaggtcc gggttgcccg ggcgatcagt
     2521 gccgctcgca cctccctcgt gggcccccat ccacagttcg gactccggtt ccgggctcgg
     2581 gatgtcacgg ccctgcaggc tggcgatgga gacgcgcgat ccccacgcgt cggagaagac
     2641 gacgggcgtc agacgatcca cggcagagca actctcgggg tccggggtgc cgctcggcgt
     2701 gcactggccg aagcgggtgg aagaacgacc tcagatgcta acgcggatca cggtgcccgg
     2761 ggccggaccg gccgggggca gtggccgacg tcaccaccgc aagtgccgtc acgacgtccg
     2821 ggtcgtactc gtagcccagt cccagttgga tccgctcgag cgcggcgtcg acggccgacg
     2881 cccccgagtc cttctgggtg aggtcgtcgt acgcgttcgc caccttgatg atccgactcc
     2941 cgagggcgac gtcctccccg agttcgcgca cccgccggta cggagtgggc accgattcga
     3001 cgatcgaggc gatgttctcc agccccgagg cccggcggat gatgcgagcg ccctcgctgg
     3061 agatctccag ctggtcactc ggtgcagcca gcaccgtggc accaccggga atgggctcgg
     3121 tcaacgagat ctggccgaga tcgtgcagca gggctgcgta ttcgacgtct cgcagctcac
     3181 ggcccgacag gccgagcacc cggcccatcc gcacgctgga gaccgcgacc cgctcagcgt
     3241 gccccgaggg ggtgtaccca ccagcctcgg tgagcccgga gaggatggcg atcgtctgtc
     3301 ggtagatctc tcggttcctc gaggcccggt ccacggccac gtacgtgatg gcgagcggga
     3361 acagcgcaac gggcagtgcc aggagtccca actgcggtgc catgagcgca accatcggtc
     3421 ccgtggtgat gaccgcatag gtgagcgggg ccacctctcc ggcctcgtcc cgcagcgcgg
     3481 aggaccacgc cgtgcgctga cgttccgagc gcaccgcgga cgacaacccg atctcgacgg
     3541 tgaggccgat ggcggcgatg acgaggaacc cggcagccac gacggtgagc ggaacctgct
     3601 cgccgacatc gaggtcccag agcggtagac cccacgggct cgctgcccgc gccaaccagg
     3661 cggccacacc gacgccgacg aggcgggccg ccatctggtc gacgcgcacc gcacgaccga
     3721 aggccaatcg caccaccgcc gcgaggacca gccccgccgc cacgaccagc acgacgaccc
     3781 cggagggcac gtcgaaggtc ggctcgcctg cgatgtcccc gacgaacgcg agcgccaagg
     3841 cggacgccga ggcgagcggc gccgcctccc gccggctcgg catccgcagt cgcaccagct
     3901 ctccggccac gagggcgacg acgaacacga cgatcgtccg gtagtgcagg cccgacgccc
     3961 agccagcact gccgaatgcc gtcgtgaggg ctgccgcggc cgtgaggatg ccgagtgcga
     4021 gcagggcggc tgctcggggt cgctcgagga gcggacgcgg gacgctcatg cgccggccac
     4081 gtttgctcgg cgcacgagag cctgctcgag ggtcggcagc aggccgtggt catccgggga
     4141 gcgggctcga agggccgcca tggcctcgtc atgggtgagc acacggccat cagggctacc
     4201 gacctcggtg aggaggtcgt actcgtcggc cagtccgacc accagcgctg gcaaccccag
     4261 ggctgcgccc tcggcgcccg acccgacggc ctcgcggtgg tgggcgacgg catccaccga
     4321 tccggcgagg aaaccgactt cctgcaccag gcgagcacct cgctgcgggt agtcgcccag
     4381 cgcgacgctg gcaccgacgg attcgcggcg caccgtcgcc gtcggcagcg tcgtcaggcc
     4441 cagatcgtga agcatgcccg cggcgcggat gtcggcgacg acggcagggc gcagtccgag
     4501 cgactcagcc atgtgcacac tgagctgggc cacccgcgcg ctgtgtccga ccaggtgcgg
     4561 cgccttggac tccacagcgc cgaccagcac cccgagggcc cgctcatggc ccttgacctc
     4621 gtcgctgtac tggttgaagg cccaccgggc cacgagcagt ggtgggagca cgaggacgac
     4681 cccgaacggg ccgagaccca ccggctccca gagcaccacc aggatgaacg cgatgacgcc
     4741 gtagccgagc tggttcgggc cgctcgtgac cagcaggcgc cagatctggg tgcgcatcgg
     4801 gacaccctgg gccagcctga cgaccatggc gatgagcagc aggttggcaa gcgcctgcgc
     4861 gacatcggcg accaggatcg ggagaccgac ggcgcgagcg atctcccacg gacccgtgag
     4921 gcccgcaccc ccgaccaccc cgccggcagc ccggtacacg acgcccccga agaccccgag
     4981 cagggcgaac atgccggtgt tgaacaggcg agcccgccac ggctg
//
LOCUS       DAOSOC010000174         4929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_17242_length_4929_cov_7.099508, whole genome shotgun sequence.
ACCESSION   DAOSOC010000174 DAOSOC010000000
VERSION     DAOSOC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17242_length_4929_cov_7.099508"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..>2191)
                     /locus_tag="PL014_11115"
     CDS             complement(234..>2191)
                     /locus_tag="PL014_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT57683.1"
                     /translation="VNMSIGGLPALNDGNNARARLYDALIDEYGVQLFISAGNSGAGI
                     NTIGDPSVATSVVSVASSVSKETWLSNYGSVVSTPLTLHNYSSRGPREDGGFKPNVMA
                     PGSAVSTVPQWLEQPGVAETGYTLPVGYAMFNGTSMASPQAAGGAALLLSAGFATGTP
                     ITPAQLRESMYTTADFVKGVEAVSQGNGQIDVPASWNLLKTKPATRTFTTDAPVCTPI
                     SDFLATPDRGTGVYNRCAAGDGGHKVGQSKTYRVKVTRTSGASGNVAHRLRLIGNDGT
                     FTVPSAASLRLDRAQSITVVAKPRTAGLHSAILVVDDPKTGVVDHRVMLAVVASEDLT
                     KPSYALTKTGEVERNLVKRHFVTVPEGTKALQVNLGGIATNSQVRWIAFNPYGVPVDP
                     TATTQCYTNFPGSPCNANSRAYSNPIPGVWELIVESRRTSPFLDNPYRLTAAAQGVTV
                     DPAIQTIAAPIGEATAVSWTVRNDFGPVTVTPKGGNLGSALSQRPSIADGDVQEFEVV
                     VPAGAERLDVSIGNPADLSADLDLSVYNAAGELVAQDADGDSDESVSLANPAPGTYTV
                     EVDGYSVPAGTTEYDYLDVFFSSALGTLTVPGAAITLDGGQSATVTGSLTATAPAAAG
                     RQLFGEMNVVTAAGAVLGTGSVLVSAP"
     assembly_gap    2192..2291
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2292..2764)
                     /locus_tag="PL014_11120"
     CDS             complement(<2292..2764)
                     /locus_tag="PL014_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57684.1"
                     /translation="MPRRSTQPARTALAALAAAALAAGLVSTTATSAGAAEPEPRAKV
                     TAPKASADKGTLKKKASKDKLGQHDRQLLEKARSKGEKRVTILLATEKGATKGVAASI
                     KANGGFTGMTNDRIGYVRATVPTSAVEKVAGLAKVLAVDLNESIPLPDPTVESSGR"
     gene            complement(2919..4028)
                     /locus_tag="PL014_11125"
     CDS             complement(2919..4028)
                     /locus_tag="PL014_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT57685.1"
                     /translation="MTRLNRLAIAAGAASALGALAAAGRGVNAAMGGRPAGARLERMK
                     RSAAYRAGAFHNTSRGTSSMPSGQRDVLRRFRDSAETRKPARPIPVVSASHEPRPDGL
                     HITWFGHASCLVELDGARILLDPVWSDRCSPSQHVGPKRLHAVPVPLTGLGRVDAVVI
                     SHDHYDHLDMDTIRQLCQLTSAVFVVPLGVGAHLETWGVSAHRIVECDWEDGHDVAGM
                     RITAVEAQHFSGRGLRRDGTLWASWVLSGAGGRVFFSGDTGYFDGYPRIGAEHGPFDA
                     LLMAVGAYDPAWHDIHLDPEESVEAAVAMGGGLVLPIHWCTFVLAPHPWAEPVERLLA
                     AADTAGVEVTVPKVGQRIDPAEPPAADPWWAQLDA"
     gene            complement(4039..4782)
                     /locus_tag="PL014_11130"
     CDS             complement(4039..4782)
                     /locus_tag="PL014_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850450.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOT57686.1"
                     /translation="MGVDRPVIGVSCYVEDVDRAPWVAQRSAVLPHGYVDHLERAGAL
                     VVVLPPRPDADDDLAAAVLARLDGLVIAGGADVEAARYGAPPHVTSQDPRPDRDAWEL
                     ALARVSKVARMPVLGICRGMQVMAVAGGAALEQHLPDVVGNDEHCPAPGVYAHHLVTP
                     VSGTRLAGILGDGPLDVPTYHHQAVRPSSLEGTGYRAAAWHDDGTLEAMEDPASLFRV
                     AVQWHPEAGADPRLFDELVASARQGGDRG"
     gene            complement(4796..>4929)
                     /locus_tag="PL014_11135"
     CDS             complement(4796..>4929)
                     /locus_tag="PL014_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT57687.1"
                     /translation="GRFGEPEEMANAVLFLASDESSFMTASTFLVDGGISGAYVTPL"
BASE COUNT          705 a         1637 c         1766 g          721 t
ORIGIN      
        1 cgctcccgac aagcaaaaga ccccccgggt gcgggaggtc tgcgcgacga ggagttctcg
       61 tcgcgctagc cgataatgag cgtggcggtg gccacaggaa gcatcacccc cacactgtcg
      121 cacgggtcag gggccggggg acagcaggag tccacatgct ggcgaccgcg gacgcacgga
      181 cagcagaagg cggcgacccc cgatgtgggg gccgccgcct ttcccggatg gaatcagggt
      241 gcggagacga gcaccgaccc ggttccgagc acggcaccgg ccgcggtgac gacgttcatc
      301 tcaccgaaga gctggcgccc ggccgcggcc ggagccgtcg ccgtgagcga gcccgtgacc
      361 gtggccgact gcccaccatc gagggtgatg gcggcgccgg gcaccgtgag cgtcccgagc
      421 gccgacgaga agaacacgtc gaggtagtcg tactcggtcg tgcccgcggg cacggagtac
      481 ccgtcgacct ccaccgtgta cgtcccgggc gccgggttgg ccagcgacac cgactcgtcg
      541 gagtcaccgt cggcgtcctg ggcgaccagc tcaccagcgg cgttgtagac cgacaggtcg
      601 aggtctgccg agaggtcggc ggggttgccg atgctcacgt cgaggcgctc ggcacctgcc
      661 ggaaccacca cctcgaactc ctggacgtcc ccgtcagcga tgctcgggcg ctggctcagg
      721 gccgagccga ggttgccacc cttgggtgtg accgtgaccg ggccgaagtc gttgcgcacc
      781 gtccacgaga ccgcggtggc ctcgccgatg ggggcagcga tggtctggat cgccgggtca
      841 accgtgacgc cctgggcggc agcggtcagg cggtacgggt tgtcgaggaa cggcgaggtg
      901 cgccgcgact cgacgatgag ctcccagaca cccgggatcg ggttgctgta cgcccgcgag
      961 ttcgcgttgc acggcgagcc ggggaagttc gtgtagcact gcgtcgtcgc cgtcgggtcc
     1021 accgggaccc cgtacgggtt gaacgcgatc caacgaacct ggctgttcgt cgcgatgcca
     1081 ccgaggttga cctggagggc cttggtgccc tcgggcacgg tcacgaagtg gcgcttgacg
     1141 aggttgcgct cgacctcgcc ggtcttggtc agcgcgtacg agggcttcgt caggtcctcg
     1201 gacgccacca cggcgagcat gacgcggtgg tcgacgacgc cggtcttcgg gtcgtcgacg
     1261 acgaggatcg cggagtgcag accggccgtg cgcggcttgg ccacgacggt gatgctctgc
     1321 gcgcggtcca gacgaaggga tgccgccgag ggaaccgtga acgtgccgtc gttgccgatc
     1381 agacggaggc ggtgagccac gttgcccgag gcaccggagg tgcgggtcac cttgaccctg
     1441 taggtcttgc tctggccgac cttgtggccg ccgtcgcccg cggcgcagcg gttgtagaca
     1501 ccggtgccac ggtcgggggt ggccaggaag tcggagatcg gcgtgcacac cggggcgtcg
     1561 gtggtgaacg tgcgggtcgc cggcttggtc ttcagcaggt tccaggaggc cgggacgtcg
     1621 atctgaccgt tgccctggga gaccgcctcg acgcccttga cgaagtcagc cgtcgtgtac
     1681 atcgactccc gcagctgcgc gggggtgatc ggcgtgcccg tggcgaaacc ggccgagagc
     1741 agcagtgctg caccaccggc ggcctgcggg gaggccatcg acgtcccgtt gaacatcgcg
     1801 tagcccaccg ggagggtgta gccggtctcc gcgacgccgg gctgctcgag ccactgcggc
     1861 accgtcgaga ccgccgaacc cggggccatg acgttgggct tgaagccgcc gtcctcacgc
     1921 gggccgcgtg aggagtagtt gtgcagcgtg agcggggtgg agacgaccga gccgtagttg
     1981 gacagccagg tctccttgct gaccgacgag gccacgctga ccacgctcgt cgcgaccgac
     2041 gggtcgccga tcgtgttgat gcccgcgccg ctgttgccgg cagagatgaa cagctgcacg
     2101 ccgtactcgt cgatgagcgc gtcgtagagg cgggcccggg cgttgttgcc gtcgttgagg
     2161 gcgggcaggc cgccgatcga catgttgacg annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn ncgtccggag ctctcgaccg tcgggtcggg cagcgggatc gactcgttga
     2341 ggtcgacggc cagcaccttg gccaggccgg cgaccttctc gacggccgag gtgggcacgg
     2401 tcgcgcgcac gtacccgatg cggtcgttgg tcatgccggt gaagccgccg ttggccttga
     2461 tcgaagcagc aacccccttc gtggcgccct tctcggtcgc cagcaggatg gtcactcgct
     2521 tctcgccctt gctgcgggcc ttctcgagca gctgacggtc gtgctggccc agcttgtcct
     2581 tgctggcctt cttcttcagc gtgcccttgt cggcgctggc cttgggtgcc gtgaccttgg
     2641 cccggggttc gggctcggcg gcacctgcgg acgtggccgt ggtcgagacc aggcccgccg
     2701 cgagggcagc cgcggcaagg gctgcgagcg ctgttcgcgc tggttgggtg gaacgtcgtg
     2761 gcattggcgc atcctgtcgg gaagggtggt gagtgaaccc cagggtcggt tggtgcgcgc
     2821 acagtgcacc atgacgccct ctccaacgga ggatgtttcc cgatttctga ccattccttt
     2881 accttttgcg gcgtcgcccc tgacgttcgc gtcaagcgtc aggcgtcgag ctgggcccac
     2941 cacgggtctg ctgccggcgg ttcggccgga tcaatgcgtt gccccacctt gggcaccgtg
     3001 acttcgaccc cggccgtgtc cgcggcggcg agcagccgtt caaccggctc ggcccaggga
     3061 tgcggcgcga gcacgaaggt gcaccagtgg atcggcagca ccagcccgcc gcccatcgcc
     3121 acggccgcct cgacggactc ctccgggtcg aggtggatgt cgtgccaggc cgggtcgtag
     3181 gcgccgacgg ccatcagcaa cgcgtcgaac ggaccgtgct cggcgccgat ccgggggtac
     3241 ccgtcgaagt accccgtgtc gcccgagaag aacacccgac cgccggcccc ggagagcacc
     3301 cacgacgccc agagcgtgcc gtcgcgccgc agaccgcgac cggagaagtg ctgggcctcg
     3361 acggcggtga tccgcattcc cgccacgtcg tgaccgtcct cccagtcgca ctcgacgatg
     3421 cgatgggcgg acacccccca cgtctcgagg tgggcaccga cgccgagcgg aacgacgaag
     3481 acggcactgg tcagctggca gagctgacgg atggtgtcca tgtcgaggtg gtcgtagtgg
     3541 tcgtgcgaga tgacgacggc atcaacccgg cccagaccgg tcagcggcac cggcaccgcg
     3601 tgcagccgct tgggcccgac gtgctggctc gggctgcaac ggtcgctcca gaccggatcg
     3661 agcaggatcc gagcaccgtc gagctcgacc aggcacgacg cgtgcccgaa ccaggtgatg
     3721 tgcagaccgt ctgggcgcgg ctcgtgcgaa gcactgacga ccgggatcgg ccgggccggt
     3781 ttgcgggtct cagcgctgtc ccggaagcgg cgcagcacgt cccgctgccc tgagggcatc
     3841 gaggaggtcc cccggctggt gttgtggaac gcccccgcgc ggtaggccgc cgagcgcttc
     3901 atccgctcga ggcgggcacc agccgggcgc ccgcccatgg cggcgttcac ccctcgtccg
     3961 gcggcggcga gggcaccgag ggcactggct gcacccgcgg cgatggcaag tctgttgagc
     4021 ctggtcaccg ggtgattgtc acccgcggtc gccgccctgt ctcgcagacg cgacgagctc
     4081 gtcgaacagt cgcgggtccg cgcccgcctc ggggtgccac tgcacggcca cccggaacag
     4141 cgatgccggg tcctccatcg cctcgagcgt gccgtcgtcg tgccaggcgg cggcccggta
     4201 gccggtgccc tcgagtgagg agggccgcac ggcctggtgg tggtaggtcg gcacgtcgag
     4261 cggcccgtcc ccgaggatgc cggccagccg ggttccgctg acgggtgtca cgaggtggtg
     4321 ggcgtagacc cccggcgcgg gacagtgctc gtcgtttccg acgacgtcgg gcaggtgctg
     4381 ctcgagggcc gcaccgccgg ccacggccat gacctgcatc ccgcgacaga tgccgagcac
     4441 cggcatccga gcgaccttcg acacccgggc gagcgcgagc tcccaggcgt cacgatcggg
     4501 ccgcgggtcc tgcgaggtga catgcggtgg ggcgccgtag cgcgccgcct cgacgtccgc
     4561 tccccctgcg atgacgaggc cgtcgaggcg agcgagcacc gctgccgcga ggtcgtcgtc
     4621 agcgtccggt cgcggtggca ggaccaccac gagcgcccct gcgcgctcga ggtggtccac
     4681 gtacccgtgc ggcagcaccg cactgcgctg ggccacccag ggggcccggt cgacgtcctc
     4741 gacgtagcac gagacgccga tcaccggccg gtcgacaccc atcaccagtt cgtcatcaca
     4801 gcggcgtgac gtaggcgccc gagatgccgc cgtcgacgag gaaggtcgac gccgtcatga
     4861 agctcgactc gtcggaggcg aggaagagca cggcgttggc catctcctcg ggctcgccga
     4921 agcggccca
//
LOCUS       DAOSOC010000175         4918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_17291_length_4918_cov_8.156282, whole genome shotgun sequence.
ACCESSION   DAOSOC010000175 DAOSOC010000000
VERSION     DAOSOC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17291_length_4918_cov_8.156282"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3325)
                     /locus_tag="PL014_11140"
     CDS             complement(<1..3325)
                     /locus_tag="PL014_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HOT57688.1"
                     /translation="MTHHGLLDLVVTGGPDAGRAHPLAPPGVSIGRAPVAGLALADHA
                     LSRTHAVVDVGPSGVSVRDAGSTNGVFVDGTLVTETTTVDAASTIVVGSSTLRLRRAS
                     GRGLPTRSPGDGTLIVQPSTWPAPDPGPVEIVCPPTPGERHRVRIPWIAALVPVPIGV
                     GLAFLLGPYLLIFALMGPLVLLTSALGDRWGAGRAHRRAVAAHAVAVHAARDRLRAAL
                     VDETARLHRGHPDPHQVLRVAEGALPGLWSAGPSDGALPIRLGTGSVATRVVWTDGPD
                     RVNPAADNAPVVLDLAACRAVAVVGPPLVTDGHLRMLLGQVCTTSPPQRLSIAVVAPD
                     TSWAWVDLLPHAHDVADLLAPPGPASASTHHRVLVIPEPAKVVGGVHPLITRALDADV
                     LVVVACTDRAEVPEGVSTVVEPGGNGRHVLHHDAKPEDVDVVIDAVGPWWADRVARAL
                     APLRSAEGGVGSLPRSISLSQAVAADLSPPALAARWRARARSGTPTAVVGVTADGLFS
                     IDLVHDGPHVLIGGTTGSGKSEFLRTLVTSLALACPPEDLTFVLVDFKGGAAFGPCAS
                     LPHVVGLVTDLDEHLVSRALRSLGAELRRRERIFATVGASDLEGYHRAQGPGTESVPR
                     LVIVIDELKALVDEVPDFVSGLVRLAALGRSLGVHLVLATQRPSGAVTAEIQANVNLR
                     IAFRVRDRTDSVDILEDPAAAGIRSSTPGRALSRGGDGILVMFQAATLGDGDSAAEPF
                     LRVSAPDVQEDARMPAPSVHAVTPLVDAARRAHALRGGAAPRTPWLPPLPDLVHPVSE
                     PSIPAHDPAPRATIGLVDEPEHQQVSPLVWTPGDGSWLLSGRPGSGRTTALRTLVLSL
                     ARRLPSDRLHIHVIDPSAALADLEALPHVGTRLTPTRRRSLAALIVHLRHEVEVRRST
                     PDQAPDAPLVVLVIDGWEQFTEAQGALDPEGVVDAVLAILRDGSSVGVVGVVAGGRGL
                     LQPRWSAIGGTTLLLGAVDPLDAALVGLRLADVPRDPPRGRAVRLADRREVQIALSMA
                     QDSCEVAAHASRGGTGRSTDAGQPWAWRDLPDIAHRPAGGPSGADGHHHDATPGPTPL
                     GLGLRAPSHEVWRWDPSDIG"
     gene            complement(3888..4313)
                     /locus_tag="PL014_11145"
     CDS             complement(3888..4313)
                     /locus_tag="PL014_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT57689.1"
                     /translation="MEQSITVDIAAPSEQVWEVLSDVEYWSEWTSTVTSVHRLDEGPL
                     RTGSRAKIRQPRIPENEYVVTELEPGRSFTWVATGPGVLTTARHDAEPLPGGGTRVRL
                     SVSQTGLLGSVMGRFYRGLTDRYLANEANGLKARCEQQM"
BASE COUNT          715 a         1680 c         1859 g          664 t
ORIGIN      
        1 gcccgatgtc cgaggggtcc caccgccaca cctcgtggct gggcgcgcgc aggccgaggc
       61 cgaggggcgt cgggccgggg gtcgcgtcgt ggtggtgtcc atcggcgccg ctcgggcccc
      121 ccgctggtcg atgcgcgatg tccggcaggt cgcgccacgc ccacggctgg ccggcgtcgg
      181 tgctccggcc ggtgccgccc cgactggcgt gcgcggcgac ctcgcaggag tcctgcgcca
      241 tcgacagggc gatctgcacc tcgcggcggt ctgccaaccg aacggcccgc cctcggggtg
      301 ggtcgcgggg gacgtccgcc agtcgtagac cgaccagggc ggcatccagc ggatcgactg
      361 ccccgaggag cagggtggtc ccgccgatcg ccgaccatcg aggctggagc aggccgcgcc
      421 cgccggcgac cacgccaacc accccgacag acgagccgtc gcgcaggatc gcgagcacgg
      481 catccacgac accctcgggg tcgagggccc cctgcgcttc ggtgaactgc tcccacccgt
      541 cgatcaccag gacgacgagg ggtgcgtccg gcgcctgatc gggggtcgat cgtcgtacct
      601 ccacctcgtg tcggaggtgg acgatcaggg cggcgaggct gcgccgacgc gtcggtgtga
      661 ggcgtgttcc cacgtgaggg agggcctcga ggtctgcgag cgcggcgctc gggtcgatga
      721 cgtggatgtg caggcggtcg gacggcagac ggcgcgcgag ggagagcacc aacgtccgca
      781 gcgcagtggt gcgaccgctg ccgggccggc cggacagcag ccacgagccg tcaccgggcg
      841 tccacacgag cggcgagacc tgctgatgct cgggttcatc gaccaacccg atggtggccc
      901 tcggggccgg gtcgtgcgcc ggtatcgatg gctcgctgac ggggtgcacg aggtcgggca
      961 ggggcgggag ccacggtgtg cggggcgcgg caccgccgcg cagggcatgg gcccgccgcg
     1021 ccgcatcgac caggggggtg acggcgtgca ccgacggagc cggcatccgt gcgtcctcct
     1081 gcacgtcagg cgcgctcacc ctgaggaacg gctcggcagc gctgtctccg tcaccgaggg
     1141 tcgcggcctg gaacatcacg aggatgccgt cgccgccgcg gctgagggcg cgtcccgggg
     1201 tgctcgagcg gatgccggcc gcggccgggt cctcgaggat gtcgacggag tcggtgcggt
     1261 ctcggacccg gaaggcgatg cggaggttga cgttggcctg gatctcggcg gtgaccgcac
     1321 ccgacggccg ctgggtggcg agcacgaggt ggacgcccag cgagcggccg agggcggcca
     1381 ggcgcacgag tccgctgacg aagtcgggca cctcgtcgac cagagccttg agctcgtcga
     1441 tgacgatgac gaggcggggc acgctctccg tgccggggcc ctgggcgcgg tggtacccct
     1501 cgaggtcact cgcccccacg gttgcgaaga tccgttctcg ccgccgcagc tcggccccga
     1561 gggaccgaag ggcgcgggac acgaggtgtt cgtcgaggtc ggtgaccagc ccgacgacgt
     1621 gggggagtga ggcgcacggg ccgaaggcgg cgccgccctt gaagtcgacg aggacgaacg
     1681 tcaggtcctc gggtgggcag gccagggcga gcgaggtgac cagggtgcgc aggaactcgg
     1741 acttgcccga tccggtcgtg ccgccgatca gcacgtgcgg gccgtcgtgc accaggtcga
     1801 tcgagaacag cccgtcggcc gtgacgccga ctacggccgt cggcgtgccg gatcgagccc
     1861 gtgcccgcca ccgggcggcc aatgctggcg ggctgaggtc ggcggcgacg gcctgcgaca
     1921 gggagatgga tcgggggagg gagccgaccc cgccctcggc agaccgcagc ggggccaggg
     1981 cccgcgcgac ccgatcagcc caccacgggc ccacggcgtc gatgacgacg tcgacgtcct
     2041 ccggtttcgc gtcgtggtgc aggacgtggc gcccgttgcc gcccggttcc accaccgtgc
     2101 tcaccccctc gggcacctcg gcgcggtcgg tgcacgcgac gaccacgagc acgtcagcat
     2161 ccagggcgcg ggtgatgagg gggtgcaccc caccgacgac cttcgccggt tcggggatga
     2221 cgagcacccg gtggtgggtc gacgccgacg ccgggcccgg gggtgccagg aggtccgcca
     2281 catcgtgcgc gtgggggagc aggtcgaccc aggcccacga ggtgtccggc gccaccacgg
     2341 cgatggacag ccgctgcgga gggctcgtcg tgcacacctg gccgaggagc atgcgcaggt
     2401 gaccgtcagt gaccagtggg gggcccacga ccgccacggc tcggcaggct gcgaggtcga
     2461 gcacgaccgg agcgttgtcg gcggcagggt tgacccggtc cgggccgtct gtccagacca
     2521 ctcgggtggc gaccgagccc gtgccgagtc gaatcggcag cgcgccgtcc gagggcccgg
     2581 ctgaccagag gcccgggagt gctccctccg cgaccctgag cacttggtgg gggtcggggt
     2641 ggccccggtg cagccgggcc gtctcgtcga ccaacgctgc gcgaaggcgg tctcgcgccg
     2701 cgtggacggc cacggcatgt gctgccacgg cacgtcgatg ggcccgcccc gccccccatc
     2761 ggtccccgag cgcgctggtc agcaggacga gcgggcccat cagggcgaag atcagcaggt
     2821 aggggccgag gaggaacgcc agtcccaccc cgatcgggac ggggaccagg gccgcgatcc
     2881 acgggatgcg gacacggtgc ctctcaccgg gagtcggcgg gcagacgatc tcgacaggtc
     2941 ccgggtcggg ggcgggccac gtcgagggtt gcacgatcag ggtcccgtca cccggggacc
     3001 gggtgggcag gccgcgccct gatgcccgac gcagccgcag ggtcgaggat ccgacgacga
     3061 tcgtcgacgc ggcatccacc gtagtggtct cggtgacgag ggtgccgtcg acgaacaccc
     3121 cgttggtcga cccggcatcg cgcacgctga ctccggacgg accgacgtcg accaccgcgt
     3181 gggttcgact cagggcatgg tcggccaggg ccagcccggc gacgggtgca cggccgatgc
     3241 tcacgccggg cggcgcgagg gggtgggcgc ggccggcatc cggcccgccc gtgacgacga
     3301 ggtcgaggag gccgtggtgc gtcacggttc gaggcaaacc accggcggcg ccagcgactg
     3361 gggcgacgtg ggcggtacca gcccgaccaa gcggaccaag ctccgcctcg ggtggtggga
     3421 cccccgccac caccaccgag gccccgtgaa ccaggggtgg caaccccacg acggcgctcg
     3481 catcgagaag ctgccctcgg ctggacaggc cgtccacgga ctctccgagg tgctccgcca
     3541 gtgcccggca gaggtcgccc gcggtgtgct ctcccaccca ctcgacgcag acgtcgacag
     3601 gtagtggcct gcgccgcggc acgtgcacgg tgagggtcag gcgcacgtcg tcatctccat
     3661 gtcgtcccct cagatggtcc cccggtgaca gggcccccca cggtcccgtc cgtgacccac
     3721 cgtaagtgcc cgggaggccg ttcctgaggt tgtccacagg ttcgcaggag acgtcaggcc
     3781 aaccaccgtc aacctgccag cgttgaaaga ccttcagccc acggctggat ggttgatgtt
     3841 cctcagatgc gtctcattgc cgccagatgg ccacggggaa tgcggcccta catctgctgt
     3901 tcgcacctgg ccttgaggcc gttggcctcg ttggcgaggt agcggtcggt cagtccgcgg
     3961 tagaaacgcc ccatcaccga ccccaacaag ccggtctgcg acaccgacag ccggacccgt
     4021 gtgccgccgc cgggcagggg ctcggcgtcg tggcgggcgg tcgtgagcac ccccggaccg
     4081 gtcgccaccc aggtgaacga tcgcccaggc tcgagctcgg tgacgacgta ctcgttctcg
     4141 ggtatgcgtg gctggcggat cttcgcacgg cttcccgtgc gcaacggacc ctcgtcgagc
     4201 cggtgcaccg acgtcacggt cgacgtccac tcggaccagt actcgacgtc gctcaacacc
     4261 tcccacacct gctcggacgg cgccgcaatg tcaacggtga tgctctgctc catcggccca
     4321 tcctgacgtg caaggcaacg gcatcgccac cagcccacac cgtggaggtg gccgcgtctc
     4381 agcgccgcct agacgcgcgc gtggggtgtc gcgggccggc tggggatcgg atgcgtggct
     4441 ctgcaggagc ggccggttga aggacgccag tggtgacgtt gaaggaccct gcgtcgtttg
     4501 cgttcgccca aacgacggag ccaccacgcg atgacatgtc ggggcttgaa tctcacgtcc
     4561 atgtcggtgg gaagtggaag cggtcgatca gcaggctcgc ggaatgcacc cgcggattcg
     4621 ggaccgcttc gacctcaccc tggagtggat cccgccgctc atacatgggc gccgagagcc
     4681 cttcgccagc gcgctgagtg gctacggaga cttcttcggg ctcttgagga cttcccgggg
     4741 atgtcgttga tggttcacgg gatgcgcgga gcgtctctgt gccctgacgg cagcgcgggg
     4801 tgacgcgcac ggcatcgggg ggttatccac aggttgacgt tctcgggatg cggtgtgggc
     4861 tgctctcagg ttcgatgtga gtctgatggt cgtcgagggg cggtcgcggg ggagggag
//
LOCUS       DAOSOC010000176         4915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_17316_length_4915_cov_7.994033, whole genome shotgun sequence.
ACCESSION   DAOSOC010000176 DAOSOC010000000
VERSION     DAOSOC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17316_length_4915_cov_7.994033"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..981)
                     /locus_tag="PL014_11150"
     CDS             complement(214..981)
                     /locus_tag="PL014_11150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT57690.1"
                     /translation="MSEVRDAEIALNRAVWAVVNERFTDAAAHDQWVRPEPVWGLFAV
                     PERELGVLGDVRGLDVVELGGGTAYVSAWLARAGARPVAVDLSSEQLATARRLQVELG
                     PEFPLVQADGERLPLADGCADVVVSEHGVGAWCDPQRWLPEAARLLRPGGRLAFLTNS
                     HLSALCVPDDEGVAGETLLRGHREAYRVHWEGGGVEFHPSHGDWVRLLRASGFVIEAL
                     HEIYAPDDGSDHEFYEIVSQRWAGRWPAEELWVATRG"
     gene            complement(1042..3081)
                     /locus_tag="PL014_11155"
     CDS             complement(1042..3081)
                     /locus_tag="PL014_11155"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HOT57691.1"
                     /translation="MSEYPHLLAPLDLGHVTLPNRVVMGSMHTGLEDHARDFPKLAAY
                     FAERARGGAALIVTGGFAPSIEGTFYPLASKMTTRREARQHRQVTDAVHAEGGRIALQ
                     ILHAGRYAYTPWCVAPSAVKSPISKFKPRALSDRGVRRQIRGFARAARLAREAGYDGV
                     EIMGSEGYLINQFLAPRTNHRTDTWGGTPEKRRRFAVETVRAVREAAGPDFIVIYRIS
                     VLDLVEEGQTWDEVTALAREVEAAGASILNLGIGWHEARVPTIVTSVPRAAFAEYAGR
                     LKEHVSVPVVATNRINMPEVAESVLASSGADLVSLARPFLADPQWVRKAAESRSDEIN
                     TCIACNQACLDHTFVNKRATCLVNPRAGYETELVLGPTRTRKKIAVVGAGPAGLAAAT
                     TLADRGHTVELFEARDHVGGQFDIAMRIPGKEEFAETIRYFTRQLDLGGVKVHLGRKV
                     GADELADAGFDEVVVATGVEPRMPSIPGIDHPMVMTYAELVRGERVAGQRVAVIGAGG
                     IGVDVSEYLTTSHSVTLDLEAWRAEWGVGDPAQTRGGLTKPQPEASPRHVILLQRKAS
                     SIGKGLGKTSGWVHRAALKAKGVEHVTGVVSYDLIDDDGLHVSVGTGPEGATERRTYA
                     VDSVVICAGQESVRTIAHDLHALGLVTHVIGGADVASELDAKRAIRQGTEVAAAI"
     gene            complement(3197..3484)
                     /locus_tag="PL014_11160"
     CDS             complement(3197..3484)
                     /locus_tag="PL014_11160"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HOT57692.1"
                     /translation="MPCSSSRTASPSGCSPGLTCSPSCPTDASRVVDGARSPCVGEPG
                     REGDPLWWVRRYAEREGLDLSLALPAPLQLRKEAASLPQSLVELRTEASVA"
     gene            complement(3405..4781)
                     /locus_tag="PL014_11165"
     CDS             complement(3405..4781)
                     /locus_tag="PL014_11165"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HOT57693.1"
                     /translation="MKYAEHILDLVGNTPLVKLNSVTDGIAATVLGKVEYLNPGGSVK
                     DRIALAMVEAAEASGALKPGGVIVEPTSGNTGVGLALVAQRKGYSCIFVCPDKVSEDK
                     RNVLKAYGAQVVVCPTSVPPEHPDSYYSTSDRLVRETPGAWKPDQYSNPNGPLSHYAS
                     TGPEIWADTDGRITHFVTGAGTGGTISGAGRYLREVSADREGGRVRVVAADPEGSVYS
                     GGTGRPYLVEGVGEDFWPSAYDPAQVDEVVAVTDAESFEMTRRLAREEGLLVGGSCGM
                     AVVAALRVAKDLPADAVVVVLLPDSGRGYLSKIFNDDWMASYGFMRTGAERTVGDVLR
                     GKSRDLPALVHTHPTETLRDAIEILREYHVSQMPVVNAEPPVMSGEVAGSVSERALLE
                     ALFTGQAHLADPVAKHMSPGLPLVGAGEPVATARHELENADAVLVVEDGKPVGVLTRA
                     DLLSFLSD"
BASE COUNT          701 a         1753 c         1749 g          712 t
ORIGIN      
        1 gcgtggtctc cgcggtgccc agcctggtcg gtgcgttcct ggccgtccgg gccaagcgcg
       61 ccggcgccgg gacagccgcc accgtggcac tggtgatcaa catcctggtg ccgatcgggt
      121 ggatcgccct ccagatgctc atggcgtgac gaggcacggg tgccgtgtca cggcgcccgt
      181 ggcctgacgg ggcacacgag gcacacgagg cggtcacccg cgggtggcga cccagagttc
      241 ctcggccggc cagcggccag cccaccgctg ggagacgatc tcgtagaact cgtggtccga
      301 cccgtcgtcg ggcgcgtaga tctcgtgcag tgcctcgatg acgaagcccg acgcccgcag
      361 caaccgcacc cagtcgccgt gcgaggggtg gaactcgacc ccgcctccct cccagtgcac
      421 ccggtaggcc tcgcggtgac cgcgcaggag cgtctcccca gcgaccccct cgtcgtcggg
      481 cacgcacagg gctgacaggt ggctgttcgt gaggaaggcc agtcggcctc ccgggcgcag
      541 cagccgagcg gcctcgggga gccagcgctg cgggtcgcac caggccccga ctccgtgttc
      601 actcacgacg acgtctgcac agccgtcggc caggggcagt cgctcaccat cggcctgcac
      661 gagtgggaac tcggggccta gttcgacctg cagcctccgg gccgtcgcca gctgctcgga
      721 tgacaggtcg acagccacgg gtcgcgctcc cgcccgcgcc aaccatgccg agacgtacgc
      781 cgtgccgccc ccgagctcga cgacgtcgag gccacgcacg tccccgagca ccccgagctc
      841 gcgctcgggc accgcgaaga gcccccacac gggttccggg cgcacccact ggtcgtgtgc
      901 cgcggcatcc gtgaatcgct cgttcaccac cgcccacacc gcacgattca gcgcgatctc
      961 ggcgtctcgg acctcgctca cgggcacagg ctagacccgc gcccgccgac cgagtcccca
     1021 ctcacggcga agcgttcgct ctcagatggc ggcggcgacc tcggtgccct gtcggatggc
     1081 gcgcttggcg tcgagctcgg aggcgacgtc cgcgccaccg atgacgtggg tgaccaggcc
     1141 gagggcgtgc aggtcgtggg cgatggtgcg caccgactcc tggcccgcgc agatgacgac
     1201 gctgtcgacg gcatacgtgc gacgttcggt ggcgccctcg gggccggtgc cgacgctcac
     1261 gtgcagcccg tcgtcgtcga tgaggtcgta ggacaccacg cccgtgacgt gctcgacccc
     1321 cttggccttc agcgcggcgc ggtgcaccca gcccgaggtc ttgcccaggc ccttgccgat
     1381 cgagctcgcc ttgcgctgga gcaggatgac gtgacgaggg ctggcctccg gctggggctt
     1441 ggtcaagccg ccgcgggtct gcgccgggtc accgacgccc cactcggccc gccacgcctc
     1501 gaggtcgagg gtcaccgagt gcgacgtcgt gaggtactcg ctgacgtcga ccccgatgcc
     1561 acccgcgccg atgaccgcca cccgctgacc tgcgacgcgc tcaccgcgca ccagttcggc
     1621 gtacgtcatg accatggggt ggtcgatgcc ggggatcgac ggcatccgcg gctcgacccc
     1681 ggtggccacg acaacctcgt cgaaaccggc atccgccagc tcgtctgcgc cgaccttgcg
     1741 tccgaggtgc accttcacgc cgccgaggtc gagctgacgg gtgaagtaac ggatcgtctc
     1801 ggcgaactcc tccttgccgg ggatgcgcat ggcgatgtcg aactgcccgc cgacgtggtc
     1861 acgggcctcg aagagctcga cggtgtggcc gcggtcggcc aacgtcgtcg ccgcggccag
     1921 accggccggg cccgccccga cgacggcgat cttcttacgc gtccgggtcg ggccgaggac
     1981 gagctcggtc tcgtagccag cccgggggtt gaccaggcat gtggcgcgct tgttgacgaa
     2041 cgtgtggtcc aggcaggcct ggttgcaggc gatgcacgtg ttgatctcgt cggagcggga
     2101 ctcggctgcc ttgcgcaccc actgcgggtc ggcgaggaac ggccgcgcca gcgacaccag
     2161 gtcggcgcca ctactcgcga gcaccgactc ggccacctcg ggcatgttga tccggttggt
     2221 cgcgaccacg ggcacggaca cgtgctcctt gaggcgcccg gcgtactcgg cgaaggccgc
     2281 acgcggcacc gacgtgacga tggtcgggac ccgggcctcg tgccacccga tgccgaggtt
     2341 gaggatgctc gccccggcgg cctcgacctc gcgagccagt gccgtcacct cgtcccaggt
     2401 ctggccctcc tcgacgaggt cgagcacgga gatgcggtag atgacgatga agtccggccc
     2461 ggcggcctcg cgcacggcgc gcacggtctc gaccgcgaac cggcggcgct tctcgggggt
     2521 gcctccccac gtgtcagtgc ggtggttggt ccggggcgcg aggaactggt tgatgaggta
     2581 gccctcggac cccatgatct cgacgccgtc gtaaccggcc tcacgcgcca ggcgggccgc
     2641 acgggcgaat ccgcggatct gccgccgcac tccgcggtcg ctgagcgctc ggggcttgaa
     2701 cttgctgatg gggctcttca ccgccgacgg cgcgacgcac cacggggtgt aggcgtagcg
     2761 gccggcgtgc aggatctgca gggcgatgcg gccaccctcc gcgtgcacgg catccgtcac
     2821 ctgacggtgc tggcgggcct cacggcgggt cgtcatcttc gaggcgaggg ggtagaacgt
     2881 gccctcgatg ctcggggcga accccccggt gacgatgagg gcggctcccc cacgggcgcg
     2941 ctcggcgaag taggcggcga gcttggggaa gtcgcgggcg tggtcctcca ggccggtgtg
     3001 catcgaaccc atgacgaccc ggttggggag cgtgacgtgc ccgaggtcga ggggggccaa
     3061 caggtggggg tactcgctca tgtcactgct ctcgtctcac tcgggccggg gtcggcgcca
     3121 ccgccggtgc cggggtcgag ccgcttgcga tgttaccggc gggtcaacct ctgcgggttg
     3181 tgaggctgct cgcagctcac gccaccgagg cctcggtgcg cagctcgacg agcgactgcg
     3241 ggagggaggc agcctccttg cgcagctgaa gaggcgccgg cagcgcgagg gacaggtcga
     3301 gcccctcacg ctcggcgtac cgccgcaccc accacagcgg atcgccttct cgcccgggct
     3361 ctcccacgca gggagaccgg gctccgtcga cgaccctcga agcgtcagtc ggacaggaag
     3421 gagagcaggt cagcccgggt gagcaccccg acgggcttgc cgtcctcgac gacgagcacg
     3481 gcatcggcgt tctcgagctc gtgacgggct gtggcgacgg gctcaccggc gccgacgagc
     3541 ggcagaccgg ggctcatgtg cttggcgacc ggatcggcca ggtgcgcctg accggtgaac
     3601 agcgcctcga gcagggcccg ctcggacacc gacccggcca cctcaccgga catcaccggg
     3661 ggctcggcgt tgaccaccgg catctgcgac acgtggtact cgcgcaggat ctcgatggcg
     3721 tcgcgcaggg tctcggtcgg gtgcgtgtgc accagggccg gcaggtcgcg gctcttgccc
     3781 cgtaggacgt ccccgacggt gcgctcagca cccgtgcgca tgaacccgta ggaggccatc
     3841 cagtcgtcgt tgaagatctt ggacaggtag ccgcggcccg agtccggcag caggacgacg
     3901 acgacggcat ccgccggcag gtccttggcg acacgcaggg cggcgacgac ggccatgccg
     3961 caggagccgc cgacgagcag gccctcctca cgagccaggc gccgggtcat ctcgaaggac
     4021 tcggcgtcgg tgaccgccac gacctcgtcg acctgggccg ggtcgtaggc cgagggccag
     4081 aagtcctcgc cgacaccttc gacgagatag gggcgaccgg tgccaccgct gtagaccgac
     4141 ccctccgggt cggccgccac gacgcgcacc cgcccgccct cacgatcggc cgagacctca
     4201 cgcaggtagc gacccgcccc cgagatcgtg ccgcccgtgc ccgccccggt cacgaagtgg
     4261 gtgatgcggc cgtcggtgtc ggcccagatc tccgggccgg tgctcgcgta gtgcgacagc
     4321 gggccgttgg ggttggagta ctggtcgggc ttccacgccc ccggggtctc gcgcacgagc
     4381 cggtcggacg tggagtagta cgagtcgggg tgctccgggg gcaccgaggt ggggcagacg
     4441 acgacctgcg cgccgtaggc cttgagcacg ttgcgcttgt cctcggagac cttgtccggg
     4501 cacacgaaga tgcaggagta ccccttgcgc tgcgccacca gtgccagccc cacgccggtg
     4561 ttgcccgacg tcggctcgac gatgacccca ccgggcttca gggcgccgga ggcctcggcc
     4621 gcctcgacca tcgcgagcgc gatgcggtcc ttcacggacc cgcccgggtt gaggtactcg
     4681 accttgccca gcaccgtggc ggcgatgccg tcggtgaccg agttcagctt gacgaggggc
     4741 gtgttgccca cgaggtccag gatgtgctcc gcatacttca ccggttcatc ctcccatgcg
     4801 gcacgggcgc cctcgcgcat ccggagcgtg cccgaacgtg ggtgaagatc gtcggctggg
     4861 caacagtttt cacccacgtt cgcggatgcc gtgcggcggg ttcgcggatg ccgtg
//
LOCUS       DAOSOC010000177         4868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_17495_length_4868_cov_7.476210, whole genome shotgun sequence.
ACCESSION   DAOSOC010000177 DAOSOC010000000
VERSION     DAOSOC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17495_length_4868_cov_7.476210"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..2228)
                     /locus_tag="PL014_11170"
     CDS             complement(60..2228)
                     /locus_tag="PL014_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57694.1"
                     /translation="MRSRLLTSLALGAALWVVLVSAVGAVLSGFGWWSPVVAWPVAVV
                     VAVASGWWARSVPGVRMPVAASGALVAVTLGFTVWVGATHSEQVLPRRDAASNLQAAV
                     SLATTHERVVGIDAESVGGPAVLALDGVTLASPAFFQVGTPQEPAVQPQFVVGPAVVY
                     GFGWWAGGGPAALVLPAVAMGLALLALGLLVARVVSAWAGVVSALGVGLLFPVVHTAR
                     ATYSEPLAMLTLTGALLALTIAVAQSSDAVPGPVGAPGPGFVGDQSSQGRVLEGSLAW
                     PDGAGRAALVAGVLLGGTGLVRIDALREAVLLLPVLALGAVLGARWVRPALVGLLTSL
                     AVAAAAAVTLSSEYVAAIAGSLLPLVALAVVVGAASLVAVALRRRGVRMPLAPSMVRR
                     LPDVAAAGVVVVGVALASRPLWLVVRQDASDPGARYVAGMQARLGLPIDGGRTYAEQS
                     VAWLSWYLGPVALAVALVVLAVLVRRAVSSVALGRIEAWVPALVVAAGSTLLTLARPG
                     ITPDHPWADRRLLIALPLVVVLVVVAGQWLVERTAVAGAPAGRVWAGRLGAAALGLLV
                     VGPALVATWPHRAGGVERGSLSAVERVCDSLAPGDVVMAVDSRAANEWPQVVRGMCEV
                     PTLSTTSFLRRDPLALRAVVDQVSAAVGERGGRLVLLGADSPDAGAGGGAQEPEVFSG
                     VGLRAELVADVLVREDEHVLVTRPNGTDPLPVRVWLAPAD"
     gene            complement(2225..2629)
                     /locus_tag="PL014_11175"
     CDS             complement(2225..2629)
                     /locus_tag="PL014_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOT57695.1"
                     /translation="MSERTVITFGTFDVFHVGHIRVLQRAAALGDRLVVGVSADALNL
                     AKKGRAPVYSQEERMEIVASLRVVDEVFVEESLEQKRDYIIEHGADVLVMGDDWAGKF
                     DWVSDVCEVVYLPRTPSVSTTGIIEHIASLGS"
     gene            2717..3496
                     /locus_tag="PL014_11180"
     CDS             2717..3496
                     /locus_tag="PL014_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HOT57696.1"
                     /translation="MSTAHPTPAHLLRALVAADPGRPRITVYDDTESATRGERIELSA
                     RVLANWVAKGANLLRDDLDVGGGSVVQLDLPPHWRSLYWALAAWSVGAAVDVPARRRP
                     DAGGAAAAADSGDAGVDVVVTDAVDVASAATASGADVVFVTLAALARSATVPVPAGAV
                     DEARELATHGDVFDAWDEPDPSDVALRLLGDGETTYAAVVPEAAARGERLHTSTADTA
                     AFLRLALEVWAGDGSLVLSRGDAGDDVLTSRFVAEGVTRRS"
     gene            complement(3538..>4868)
                     /locus_tag="PL014_11185"
     CDS             complement(3538..>4868)
                     /locus_tag="PL014_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOT57697.1"
                     /translation="PHTDHIHFSFNWDGARMRSSWWSGVAVTTPLTNANYTPALPVTP
                     PPAPVMTASGYPLLAQGATGPEVILAQKVIGVTADGKFGPVTATALGAWQTKNNVSAT
                     KKLDNATWSKMVALKLIPARTVHPLQQYVNVVLKRGSTGTAVKALQKALGGLTVDGSF
                     GPATESKVKAYQQAKGLTVNGVVDSKVWNALIASSTTAAPAPAPAPAPAPTTHPLAKY
                     AGVTLKLWSKGEAVTAMQKAIGKLTADGSFGRLTEARVKEYQSEKKLPVTGVVDAGVW
                     KALMGTTTTTAPAPSGSTVTSLATEYTPYKGTVLKVGSSGAAVKVLQRGLGGLVVDGS
                     FGSLTLTAVKRFQTAKGLAVTGVVDAKTWAALELTTHPLLPYWGTVVKRGSTGATVVA
                     LQKALRITADGSFGPATEAAVKSVQATAKLSQTGVVGTLTWKAVEARMPR"
BASE COUNT          748 a         1839 c         1697 g          584 t
ORIGIN      
        1 aactgtagac cgactccacg gagcccacca aatcggctct cacccgggcg tgtcggcgct
       61 cagtccgcag gcgccagcca gacccgtacg ggcagcggat cagtcccgtt cggtcgggtc
      121 accagcacgt gctcgtcctc gcggacgagc acgtcggcaa cgagctcggc gcgaagcccg
      181 acacccgaga agacctcggg ttcctgggcc ccgccgccgg cgccggcgtc gggtgagtcc
      241 gcaccgagga ggacgagcct cccaccccgc tcccccactg ccgccgacac ctggtccacg
      301 accgcccgca aggcaagagg gtcacgccgc aggaacgacg tcgtcgacaa ggtcgggacc
      361 tcgcacatgc cccgcaccac ctgcggccac tcgttcgcgg ctcgagagtc gaccgccatc
      421 acgacgtccc cgggggccag ggagtcacac acgcgctcca ccgccgacag cgagcctcgc
      481 tccaccccgc ctgcgcggtg cggccaggtc gcgacgagcg ccggcccgac gacgagcagc
      541 ccgagcgccg ccgcgcccag ccggccagcc cacaccctgc ccgcgggtgc ccccgcgacc
      601 gcagtgcgct ccaccaacca ctgcccagcc accacgacga gcacgaccac cagcggcagc
      661 gcgatgagca accggcgatc ggcccacggg tggtccgggg tgatgccggg acgcgccagg
      721 gtcagcaggg tcgaccctgc tgccaccacc agcgcaggca cccatgcctc gatccggccc
      781 agcgcgactg acgacaccgc tcgacgcacg agcaccgcga ggacgacgag cgccaccgcg
      841 agcgccaccg ggccgaggta ccacgacagc cacgcgaccg actgctcggc gtaggtccgt
      901 ccaccatcga tcggtaggcc caaccgcgcc tgcatcccgg cgacgtaccg ggcaccggga
      961 tcgctcgcgt cctgacgcac gacgagccac agcggacgag aggcgagggc gactcccacg
     1021 acgaccacgc ctgccgcagc cacgtcgggg aggcgtcgaa ccatgctcgg ggcgagcggc
     1081 atccgcaccc ccctcctgcg cagggcgacg gccaccagcg acgccgcccc caccaccacc
     1141 gcgagcgcca ccagcggcag gaggctgccc gcgatggcgg cgacgtactc cgacgacaac
     1201 gtcaccgctg ccgccgcggc caccgccaga gaagtcagca acccgaccag ggcagggcgc
     1261 acccacctgg ctccgaggac cgcccccagc gcgagcaccg gcaggaggag cacggcctcg
     1321 cgcagggcat cgatgcgcac cagaccagtg ccgccgagca gcacccccgc gacgagcgca
     1381 gcccgcccgg caccgtccgg ccacgccagc gatccctcga gcacgcgacc ctgtgacgac
     1441 tggtcaccca cgaagcccgg ccccggcgcg ccaaccggcc ccggcacggc atccgacgac
     1501 tgcgccacgg cgatcgtcag ggccagcagg gcgcccgtaa gggtgagcat cgcgagcggc
     1561 tccgaatagg tggcgcgcgc cgtgtgcacc accgggaaca gcagcccgac ccccagcgcc
     1621 gacaccaccc ccgcccaggc gctgaccacc cgcgccacga ggagccccaa cgcaagaagt
     1681 gccagaccca tcgccacggc gggaaggaca agggccgccg gcccaccacc ggcccaccac
     1741 ccgaagccgt agaccacggc tggcccgacg acgaactgcg gctgcacggc cggctcctgc
     1801 ggtgtgccca cctggaagaa ggccgggctc gcgagggtca ccccgtcgag cgcgagcacc
     1861 gccggcccgc ccaccgactc ggcatcgatg ccgacgacgc gttcatgggt ggtcgcgagg
     1921 ctcacggccg cctggaggtt gctcgcagcg tcacgccggg gcaggacctg ctcggagtgc
     1981 gtggcaccca cccagacggt gaacccgagc gtgacggcga cgagggcgcc cgacgccgcg
     2041 accggcatcc gcacgcctgg aacgctgcgg gcccaccacc cgctcgccac ggccaccacc
     2101 accgcaacgg gccatgcgac caccggtgac caccacccga accccgacag cacagccccc
     2161 accgcgctca cgagaacgac ccacagggcc gccccgagcg ccagcgacgt caggaggcgg
     2221 gacctcacga acccaggctg gcgatgtgct cgatgatccc ggtggtggac accgacggcg
     2281 tgcgcggaag gtagacgacc tcgcagacgt cactcaccca gtcgaacttg cccgcccagt
     2341 cgtcacccat gacgaggacg tcggcaccgt gctcgatgat gtagtcgcgc ttctgctcca
     2401 gcgactcctc gacgaagacc tcgtcgacga cccgcaggct cgcgacgatc tccatccgct
     2461 cctcctgcga gtacacgggg gcgcggccct tcttggccag gttgagggcg tctgccgaga
     2521 cgccgacgac caaccggtca ccgagcgcgg cggcacgctg gagcacccgg atgtggccga
     2581 cgtggaagac gtcgaaggtt ccgaaggtga tcaccgtgcg ttcactcatg tcccgagcct
     2641 agtgccgggc tgttgcccgg cgggcaggcc accgaccagc gtggccctgt cgggggcgcc
     2701 ggttatggtc gcctgcgtga gcaccgcgca cccgaccccg gcccacctcc tgcgcgccct
     2761 cgtcgccgcc gaccccgggc gaccccgcat caccgtgtac gacgacaccg aatccgccac
     2821 ccgcggcgag cgcatcgagc tgtccgcgcg cgtcctcgcc aactgggtgg ccaagggggc
     2881 gaacctcctg cgcgacgacc tcgatgtcgg gggcggttcg gtggtgcagc tggacctgcc
     2941 gccgcactgg cgctcgctct actgggcgct cgcggcgtgg tctgtcggcg cggctgtcga
     3001 cgtgcccgcc agacggcgcc cggatgccgg gggtgcggca gccgctgccg actccggtga
     3061 cgctggggtc gacgtggtcg tgacggatgc cgtcgacgtc gcctcggcgg cgaccgcatc
     3121 cggggccgac gtggtgttcg tgaccctcgc ggcgctggcc cgctcggcca cggtgcccgt
     3181 tcccgccgga gcggtggacg aggcccgcga gctcgcgacc cacggtgacg tgttcgatgc
     3241 gtgggacgag ccggatccgt ccgacgtggc gctgcgcctg ctcggcgacg gcgagacgac
     3301 ctacgccgcc gtcgtgcccg aggcagccgc ccgaggcgag cggctgcaca ccagcacggc
     3361 ggacaccgca gcattcctgc gcctcgcgct cgaggtgtgg gcaggcgacg gctcgctcgt
     3421 cctctcccgc ggcgacgcgg gtgacgacgt gctcacgtca cggttcgtcg ccgagggcgt
     3481 gacgcgccgg tcctgagcgc tgaccgtcgg cccgggccgc ggtcagccgg gccgcggtca
     3541 gcggggcatg cgcgcctcga cggccttcca ggtcagggtg ccgacgacac cggtctggga
     3601 gagcttggcc gtcgcctgca cggacttgac cgcggcctcg gtcgcgggtc cgaaggagcc
     3661 gtcagcggtg atgcgtaggg ccttctgcag ggccaccacg gtcgcgccgg tggagccacg
     3721 cttgaccacc gttccccagt acggcaggag ggggtgggtg gtgagctcga gggcagccca
     3781 ggtcttcgcg tcaacgaccc cggtcaccgc gagccccttg gccgtctgga agcgcttgac
     3841 ggcagtgagg gtgagggagc cgaacgagcc gtcgacgacg agcccaccca gaccgcgctg
     3901 gagcaccttg acagccgcgc cgctggagcc gaccttgagc acggtgccct tgtacggcgt
     3961 gtactcggtc gcgagcgagg tgacggtcga ccccgacggc gcaggggcgg tggtggtcgt
     4021 ggtgcccatg agggccttcc acaccccggc gtcgacgacg ccggtgacgg ggagcttctt
     4081 ctcgctctgg tactccttga cgcgggcctc ggtcaggcgg ccgaaggacc cgtcggccgt
     4141 gagcttgccg atggccttct gcatggccgt cacggcctcg cccttggacc acagcttgag
     4201 cgtgacgcct gcgtacttgg ccagcgggtg cgtcgtcgga gcgggtgctg gggcgggggc
     4261 tggggcgggg gccgccgtcg tggagctcgc gatgagcgcg ttccagacct tggagtcgac
     4321 gacaccgttg acggtgagcc ccttggcctg ctggtaggcc ttgaccttgg actcggttgc
     4381 cggcccgaag gacccgtcga ccgtcagccc accgagcgcc ttctgcagcg ccttcacagc
     4441 agtgccggtg gacccccgct tgagcacgac gttgacgtac tgctgcagcg ggtgcaccgt
     4501 gcgggccggg atgagcttga gcgccaccat cttggaccag gtggcgttgt cgagcttctt
     4561 ggtggcggac acgttgttct tcgtctgcca ggcaccgagg gccgtggccg tgaccggccc
     4621 gaacttgccg tcggcggtga cgccgatgac cttctgggcc aggatgacct cggggccggt
     4681 ggcaccctgc gcgagcagcg ggtagccgct ggcggtcatg accggggccg gcggcggggt
     4741 gaccggcaga gccggggtgt agttcgcgtt ggtgaggggc gtggtcacgg cgacgccgct
     4801 ccaccacgac gaccgcatgc gggcgccgtc ccagttgaag gagaagtgga tgtggtcggt
     4861 gtgcgggg
//
LOCUS       DAOSOC010000178         4795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_17793_length_4795_cov_9.622152, whole genome shotgun sequence.
ACCESSION   DAOSOC010000178 DAOSOC010000000
VERSION     DAOSOC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17793_length_4795_cov_9.622152"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PL014_11190"
     CDS             <1..915
                     /locus_tag="PL014_11190"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOT57698.1"
                     /translation="GSAAAGTAIASGEGGAHPRERDNAAHYIFEMASGYFGWTEENIQ
                     AADAIEIKLGQGAKPGLGGTLLGPKVTEEIAAIRGVAPGTDVHSPAHFPDINSVADLT
                     HRVAEIKALTGGTPVGIKFATGHVEADLAAAIEAGADWITVDGLGGGTGAAPTHVKDN
                     VGLPSITGIRRVRRWMESEGVTDVQLIATGGFRTPDEMAKALALGADAVALATTSLMA
                     IGCQQYRACHRGTCPVGIATQDPNLRGRLDIDVSAQRLTNYLVQCTNLMSDFVRVVGK
                     TRLADLDRHDLVALDARLADHCDLEYAF"
     gene            915..1937
                     /locus_tag="PL014_11195"
     CDS             915..1937
                     /locus_tag="PL014_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57699.1"
                     /translation="MAPDDPTGPSSAPEPALDPRAEAELRLGGRYRALVLEPSPPAIG
                     DPWFADDPVRPGPLDAAGDRPLVSPVGNGDVTWSELCSDDADLAAWCADRSLAAWHPL
                     PAALPDAFVATRLTLHAVAEHVVCPARFHANGKIGLRFTRAGFGTPYFWGGGDTRQVR
                     VEAGELVVADGGGLHRYALTTLGEAAVAAGVDLGAPIEVFTPTTDADAEAPLALDPQS
                     ARLLGQWFGLAAAALEQVRADLPEADPPSRVQLWPEHFDLATDLGTETDGSRVTFGAS
                     PGDAANPEPYLYVLPWAEVERGGFWNAESFTGALLPYAALAGVDDPRAAVLQFFHQGR
                     EAIDAR"
     gene            1927..2790
                     /locus_tag="PL014_11200"
     CDS             1927..2790
                     /locus_tag="PL014_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOT57700.1"
                     /translation="MPAESHGTASADAPTPVGPPPAETRVWVRTELPDIDVGRVTTVV
                     AGDRAVAVTRTEDGWGALDNHCPHQGGPLGDGQLEGGYLICPWHAYEYHPCTGRPPAG
                     FSDGATAYAVEEREDGLYVEVPVVDGAPTLMDQMVDVMCEWGVDTVFGMVGHSNLGLA
                     EALRKAEVDGRLRFFGIRHEGAGAFAASGYAKLTGRPAACFTIAGPGATNLLTGLWDA
                     KVDRVPILALTGQVQTQVLGPGAFQEVPLAEAFAAVSEWSQTVLSASNGSAARGRHPA
                     RRVRVVGGRGV"
     gene            3201..3875
                     /locus_tag="PL014_11205"
     CDS             3201..3875
                     /locus_tag="PL014_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOT57701.1"
                     /translation="MTRLFVLLAGMVFAVVAALQVGTAPVARADGAPAGYDREVGAPC
                     RIIAAGATVRRVQAVVGVTQDGVLGPGTCDAIKSFQCTKNLTVDGVVGPITWGVMNGD
                     PVPAGTRVCANLTRQTLWVAKGGQRTYTATRMRSGDPRLPNSSTTPTGNFTIYWKDKD
                     HVSSIFDSPMPYSMFFHRGQAIHGSTFTLPGSHGCINVTMDDARRVWNRAAVGTPVSI
                     WGVRPL"
     gene            3991..>4795
                     /locus_tag="PL014_11210"
     CDS             3991..>4795
                     /locus_tag="PL014_11210"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H) small subunit"
                     /protein_id="HOT57702.1"
                     /translation="MAFVERGRSTEHLMGIGDRHEGDGWMAVCALDEIESGRGVVRVV
                     NGLDLAIMRDGDGSPDDAVHALGNLCPHRGGQLGDGKVVDGKAICPLHTWDFDLRTGV
                     SPFNPADSVPTFPVRVRDGQVEVDASAVPLTAPRPPVYLGPWIRRGATDRGMYLVQHL
                     AEGAGAFVEAMGSERNEPGHDTGRRYPSYDDLVFRPAQLAQLPTMGDIPIDTTTVLGT
                     GATKPLTLDRPIFVSHMSFGALSAESKEALARGSAAAGTAIASGEGGAHP"
BASE COUNT          653 a         1773 c         1687 g          682 t
ORIGIN      
        1 ggctcggcgg cggcgggcac ggccatcgcc tccggggaag gtggtgccca cccccgcgag
       61 cgggacaacg ccgcccacta catcttcgag atggcgtccg ggtacttcgg ctggaccgag
      121 gagaacatcc aggcggctga cgccatcgag atcaagctcg gccagggcgc gaagcccggc
      181 ctgggcggca ccctgctcgg gccgaaggtc accgaggaga tcgccgccat ccgcggcgtc
      241 gcgcccggca ccgacgtgca ctcgccggcc cacttccccg acatcaactc ggtggcggat
      301 ctgacgcatc gggtggcgga gatcaaagcg ctcaccggcg gcacgcccgt cggcatcaag
      361 ttcgccaccg gccacgtcga ggccgacctg gccgccgcca tcgaggcggg ggccgactgg
      421 atcaccgtcg acgggttggg gggcgggacg ggcgcggcgc ccacccacgt gaaggacaac
      481 gtcggcctgc cttcgatcac cggcatccgg cgggtgcggc gctggatgga atccgagggc
      541 gtgaccgacg tgcagctcat cgccacgggt ggctttcgca cccccgacga gatggcgaag
      601 gcgctcgccc tcggcgccga cgccgtcgcg ttggccacca cctcgttgat ggccatcggc
      661 tgccagcagt accgggcctg ccaccggggc acctgccccg tcggcatcgc cacccaggac
      721 ccgaacctgc ggggccggct cgacatcgac gtgtcggcgc agcgcctcac caactacctg
      781 gtgcagtgca cgaacctgat gagcgacttc gtgcgggtgg tgggcaagac ccggttggcc
      841 gacctggatc gtcacgacct ggtggccctc gacgcccgcc tcgccgacca ctgcgacctg
      901 gagtacgcgt tctgatggct cccgacgatc cgaccggtcc gtcctccgcc cccgaaccgg
      961 cgctcgaccc ccgggccgag gcggagctcc gcctcggggg ccgttaccgg gccctggtgc
     1021 tcgagccctc accgccggcg atcggtgatc cgtggttcgc cgacgacccc gtccgacccg
     1081 gcccgctcga cgccgccggc gatcggcccc tcgtgtcccc ggtgggcaac ggggacgtca
     1141 cctggtccga gctctgctcc gacgacgccg atctggccgc gtggtgcgcg gaccggtcgc
     1201 tggccgcgtg gcatccgctg ccagcggcgc tgcccgacgc cttcgtcgcc acccgactca
     1261 ccctgcacgc cgtggccgag cacgtggtgt gcccggcgcg gttccacgcc aacgggaaga
     1321 tcgggctgcg cttcacccgg gccggtttcg gcactcccta cttctggggc ggcggcgaca
     1381 cccgccaggt gcgggtggag gccggcgagc tggtggtcgc cgacggcggc gggcttcacc
     1441 ggtacgcact gaccacactc ggcgaggccg cggtcgcggc gggcgtggac ctcggtgcgc
     1501 cgatcgaggt gttcaccccg accaccgacg ccgacgccga ggccccgctg gcgctcgacc
     1561 cccagtcggc gcggctgctg ggtcagtggt tcgggctggc cgcggcggcg ctcgagcagg
     1621 tgcgggccga cctccccgag gccgacccgc cgtcccgggt gcagctctgg cccgagcact
     1681 tcgacctcgc caccgacctc ggcacggaga ccgacggcag ccgcgtcacc ttcggcgcct
     1741 ctccgggcga cgccgccaac cccgagccct acctctacgt cctgccctgg gccgaggtcg
     1801 agcgcggtgg cttctggaac gccgagtcct tcaccggggc gctcttgccg tatgccgcgc
     1861 tggcgggggt cgacgacccc cgggccgcgg tgctgcagtt cttccatcag gggagggagg
     1921 ccatcgatgc ccgctgaatc ccacggcacg gcttcggccg atgcgccgac gccggtcggg
     1981 ccgccgcccg ccgaaacccg ggtctgggtc cggaccgagc tccccgacat cgacgtcggt
     2041 cgggtgacga cggtggtcgc gggcgaccgc gccgtcgcgg tcacgcgcac cgaggacggc
     2101 tggggcgcgc tggacaacca ctgcccgcac cagggcggcc cgctcggtga cggccagctc
     2161 gagggcggct acctgatctg cccctggcac gcctacgagt accacccgtg cacgggccgg
     2221 ccgcccgcgg gcttcagcga cggcgccacc gcctacgcgg tggaggagcg cgaggacggg
     2281 ctctacgtcg aggtgcccgt ggtcgacggc gctcccaccc tcatggacca gatggtcgac
     2341 gtgatgtgcg aatggggcgt ggacacggtc ttcggcatgg tgggccactc caacctcggc
     2401 ctcgccgaag cgctccgcaa ggccgaggtc gacggccgcc tgcgcttctt cggcattcgc
     2461 cacgaggggg ccggtgcctt cgccgcctct ggctacgcca agctcaccgg ccggcccgcc
     2521 gcctgtttca ccatcgccgg ccccggcgcc accaacctgc tcacggggct gtgggacgcc
     2581 aaggtggacc gggtgcccat cctggccctc accggtcagg tgcagaccca ggtgctcggc
     2641 cccggcgcct tccaggaggt cccgctggca gaggctttcg ccgcggtgtc cgagtggagc
     2701 cagacggtgc tgtcggcgtc caacggcagc gcagcgcgag gtcgtcaccc tgcgcgacgt
     2761 gtacgggtgg tcggcggtcg aggtgtgtaa tgccctcgag gtcagcgaga ccaaccagcg
     2821 agtgctcctg caccgcggcc gaacccggct tcggctagcg ctcgaggacc tcgaccactc
     2881 accatgacca gacccgacac cgacccccgt ccgctctcct gtgatgagat ggtcgaaatc
     2941 gtgaccgcgt acctcgaggg cgcgctcggt cccgacgaca cggtgagctt cgaagctcac
     3001 ctcgggctgt gcccgggctg tcggatctac ctcgaccagt tccgtgagac catacggtcc
     3061 gcgagcacgc tcgctgagtc cgaggttccc cccgccgtca tggccgatct gctcgcgtgc
     3121 ttccgggggt ggcggggtcg ccggggcgtg tcttgacccg ccgcacggcg tatggccggg
     3181 tacggccgta gcctgacctc gtgactcggc tcttcgtcct cttggccggg atggtgttcg
     3241 ccgtcgtggc ggcgctgcag gtgggcacgg cccccgtcgc ccgagccgac ggggcacccg
     3301 cggggtacga ccgcgaggtc ggcgcgccgt gccgcatcat cgcggccggt gccaccgtcc
     3361 gccgggtaca ggcagtggtg ggtgtgaccc aggacggtgt cctgggccct gggacgtgcg
     3421 atgccatcaa gtcgttccag tgcacgaaga acttgacggt cgacggggtg gtcggcccga
     3481 tcacctgggg ggtcatgaac ggcgatccgg tccctgcggg cacgcgggtc tgtgccaacc
     3541 tcacccgcca gacgctgtgg gtggccaagg gtggacagcg cacgtacacg gccacgcgca
     3601 tgcgctccgg tgaccctcga ctgccgaact cgtcgacgac gccaaccggc aacttcacga
     3661 tctactggaa ggacaaggac cacgtctcgt cgatcttcga ctcgcccatg ccctactcga
     3721 tgttcttcca tcgcggacag gccatccacg gctccacgtt cacactgccc ggaagccacg
     3781 gctgcatcaa cgtcacgatg gacgacgccc ggcgtgtgtg gaacagggcg gcggtgggca
     3841 ccccggtgag catctggggt gtacgtccgc tctgaaccgt tggccgaccc ttgcgggcga
     3901 tgcatcgggg ttgccgggtg gcgcctgtaa cggctcggcc ggtcggggga caggagcacc
     3961 gggcgtcgtc ggaagtgggg agcacgagtc atggcattcg tcgagcgagg tcgcagcacc
     4021 gagcacctga tggggatcgg cgatcgccac gagggggacg gctggatggc cgtctgcgcc
     4081 ctcgacgaga tcgagtcggg tcggggggtg gtgcgggtgg tcaacggcct cgacctcgcc
     4141 atcatgcgcg acggcgacgg gtcgccggac gacgccgtgc acgccctcgg caatctctgc
     4201 ccgcaccggg gcggccagct cggcgacggc aaggtggtcg atggcaaggc gatctgcccg
     4261 ctgcacacct gggacttcga cctgcgcacc ggcgtctcgc ccttcaaccc cgccgactcg
     4321 gtgcccacct tcccggtgcg ggtgcgcgac gggcaggtcg aggtggacgc ctccgccgtg
     4381 cccctcaccg caccccgccc gccggtctac ctcggcccgt ggatccggcg gggcgccacc
     4441 gaccggggca tgtacctcgt gcaacacctg gccgagggag ccggcgcctt cgtcgaggcc
     4501 atgggctccg agcgcaacga gcccggccac gatactggcc ggcggtaccc gagctacgac
     4561 gacctcgtgt tccggccggc gcagctcgcc cagctcccga ccatggggga catcccgatc
     4621 gacacgacca ccgtcctggg gaccggcgcg acgaagcccc tgaccctcga ccggccgatc
     4681 ttcgtcagcc acatgtcctt cggggccctg agcgccgagt ccaaggaggc cctcgcccga
     4741 ggctcggcgg cggcgggcac ggccatcgcc tccggggaag gtggtgccca ccccc
//
LOCUS       DAOSOC010000179         4747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_18013_length_4747_cov_9.569054, whole genome shotgun sequence.
ACCESSION   DAOSOC010000179 DAOSOC010000000
VERSION     DAOSOC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18013_length_4747_cov_9.569054"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..693
                     /locus_tag="PL014_11215"
     CDS             79..693
                     /locus_tag="PL014_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5701 family protein"
                     /protein_id="HOT57703.1"
                     /translation="MTASQHLQRLLDLGLADLTAVSDATLARHASALPHTPNTTLAIH
                     PSLASPRQLAPLLSRTGKEGFVVEDLTDLEQFTDVEGLAVPDAPLYLVHDIDRGDDLR
                     NWSPNEALPEITARGRTPLTLNEGISWLLQEPEHLLPGSCFMTIGTRKRTAKGLDTRT
                     PALWISGGTGRDGKERKGAPKVGWCWAGNRHTWLGFASATGRSA"
     gene            831..>1307
                     /locus_tag="PL014_11220"
     CDS             831..>1307
                     /locus_tag="PL014_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57704.1"
                     /translation="MSSFPPGWATDLAILELSGSTIEQHADHLVVRSPHNPGFHWGNC
                     VFVTDPDAVDDADRWVAVFTSAFPEAGWVAIGLTRMPDAVEAWTSHGLALELDDVLTT
                     TTLPRQTPCPEGYSVRPLAEPDWQASLARAVAENTRTREHDDDGFAQFAQRRTQSRR"
     assembly_gap    1308..1407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1589..2986)
                     /gene="fumC"
                     /locus_tag="PL014_11225"
     CDS             complement(1589..2986)
                     /gene="fumC"
                     /locus_tag="PL014_11225"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOT57705.1"
                     /translation="MTETRTERDTMGPVQVPADRYWGAQTQRSLEHFDIGRDTFVWGR
                     PVVRGLGLLKKCAALANAELGLLGADVAEAVVQAADEVARGDLDDHFPLVVFQTGSGT
                     QSNMNANEVIANRAIELLGGDLGSKTPVHPNDDVNRSQSSNDTFPTAVHVAVVLELIE
                     RLHPAVDELCAALDAKADELGDVVKVGRTHLQDATPVTLRQEIGAWAAQVRDAQGDVR
                     YAGERVLALAIGGTAVGTGLNAPAEFGPLVARHLSELTGQPVHQAENLFVQLASHDGL
                     VAVSSSLRTLAGALMKLANDVRWLASGPRTGIGELVIPENEPGSSIMPGKVNPTQSEA
                     MTMVVTRVFGNDATVGFAGTQGNFQLNVFTPVMAHAVLESIRLLADACRSFTTHCVVG
                     LSADRDRIAAHLEADLMLVTALAPHVGYDTAAAIAKQAHRDGSTLRDAALASGAVTAE
                     DYDRWVDPEAMTRPG"
     gene            complement(3095..3631)
                     /locus_tag="PL014_11230"
     CDS             complement(3095..3631)
                     /locus_tag="PL014_11230"
                     /inference="COORDINATES: protein motif:HMM:NF025379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HOT57706.1"
                     /translation="MTTTDQLRTVLRDLEAKAKEMELDKKLTTFADQADQFLRVAATR
                     AGDYAAENRERVEATLDKAGAKVDEKTDGKYRDSFAKVRAGVLTGVDWVAEQREGTGM
                     PSTSGATSAPGTTDAAATADGDVTADGGPAASDASPAAVWSDVTDAAPQDSGIQDSVI
                     QDDVVRDDPTSEGPTDPR"
     gene            3990..>4747
                     /locus_tag="PL014_11235"
     CDS             3990..>4747
                     /locus_tag="PL014_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT57707.1"
                     /translation="MPSAWQARREWRTALELASAAVRTRDLHEWPRGDGHPVIVLPGF
                     LAGPESTAFLRSHLRRLGYHVHDWRHGRNLGASPELAEELEDLLLEIHDRYQRRVSLI
                     GWSAGGIYAREMARALPDYTRSVITLGSPFRGHPASTRAWRVYRLMNRHNLEKMFTDE
                     ALATRAAPLSVPTTCIYSRNDGIVAWECCLSDPAPLTENVEVTASHLGYGHEMHTLRV
                     ICDRLAQPEGAWLPYTGHAPAVRGYVMAMVRRPDL"
BASE COUNT          670 a         1716 c         1584 g          677 t
ORIGIN      
        1 gggcacggga acatcctcac agtggacggc gttggcgtat cgttcggcta accgtacaac
       61 gtacgacaaa ggagcggcat gacggcatcc caacacctgc agcgactgct cgacctcgga
      121 ctggcggacc tcaccgcggt cagcgatgca accttggccc gccacgccag cgcactcccg
      181 cacacgccga acaccaccct ggcgatccac ccctccctgg cgagcccgcg ccagctcgcg
      241 cccctgctga gccgcacggg caaggagggc ttcgtcgtcg aggacctgac cgacctcgag
      301 cagttcaccg acgtcgaagg cctcgccgtg ccggacgccc cgctctacct ggtgcacgac
      361 atcgaccggg gcgacgacct gcgcaactgg agcccgaacg aggccctgcc ggagatcacc
      421 gcccgcggcc gcaccccgct gacgctcaac gagggcatca gctggctcct tcaggaaccc
      481 gagcacctcc tgccgggtag ctgcttcatg accatcggca cccgcaagcg cacggcgaag
      541 ggcctggaca ctcgtactcc ggccctgtgg atcagtggcg gcaccgggcg cgacggcaag
      601 gagcgcaagg gagccccgaa ggtgggctgg tgctgggcgg ggaaccgcca cacctggctg
      661 ggcttcgcgt cggccacggg ccgcagcgcc tgacgaccgg gccgcaggca ctcctcccga
      721 atgtgggtga aaaccgtcgg ctggacgacg aatttcaccc acattcgtcg cagagctggg
      781 cttcgccttc gcctcagcca ccggccgccg acctcggcta ccgtcgcgcc atgagcagct
      841 tcccaccggg gtgggccacc gacctggcca tcctcgagct cagcggttcg acgatcgagc
      901 agcacgctga ccacctcgtc gtccgctcgc cccacaaccc cggcttccac tggggcaact
      961 gcgtcttcgt caccgacccg gatgccgtgg acgatgccga ccgctgggtg gcggtcttca
     1021 ccagcgcctt ccccgaggcc ggctgggtgg cgatcgggct cacgcgcatg ccggatgccg
     1081 tggaggcgtg gacgtcgcac ggcttggccc tcgagctcga cgacgtcctc accaccacga
     1141 ccctgccccg gcagacccct tgtcccgagg gctactcggt gcgcccgctc gccgagcccg
     1201 actggcaggc cagcctggcc cgcgcggtcg ccgagaacac ccggacccgg gaacacgacg
     1261 acgacgggtt cgcgcagttc gcgcagcgtc gcacccagtc ccggcgannn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnngca cccagtgggt gatcgtcacc gaggagacga
     1441 acgcggcagg ccgggtgtac cgcggcgtgg ggttcgcccc cgacagcggc agcatctcgg
     1501 cctaccggca tccgacccac tgacccgacc gcagccacgg ggtgcgtgtg aaaaccggcg
     1561 gcatgtggac ggttctcaca cacaaccatt agccgggccg ggtcatggcc tccgggtcga
     1621 cccagcggtc gtagtcctcg gcagtgaccg cacccgacgc caacgcggca tcccgcagcg
     1681 tcgagccgtc acggtgcgcc tgcttggcga tggccgccgc ggtgtcgtag ccgacgtgtg
     1741 gcgcgagtgc cgtgacgagc atgaggtcgg cctcgaggtg ggccgcgatc cggtcacggt
     1801 cggccgagag ccccacgacg cagtgcgtcg tgaacgagcg gcacgcgtcg gccagcagcc
     1861 gaatcgactc cagcaccgcg tgcgccatga cgggggtgaa cacgttgagc tggaagttgc
     1921 cctgcgtgcc ggcgaacccc actgtcgcat cgttgccgaa gacccgggtg accaccatcg
     1981 tcatcgcctc ggactgggtc gggttgacct tgcccggcat gatcgacgaa ccgggctcgt
     2041 tctcggggat cacgagttcg ccgatgccgg tgcgcggccc ggacgccagc caccgcacgt
     2101 cgttcgcgag cttcatcagc gcccccgcga gcgtgcgcag cgacgacgac acggcgacga
     2161 gtccgtcgtg cgacgcgagc tgcacgaaga ggttctcggc ctggtggacg ggctgcccgg
     2221 tcagctcgct gaggtgtcgg gcgacgagcg gaccgaactc cgccggggca ttcagcccgg
     2281 tgcccaccgc ggtcccgccg atggcgaggg cgagaacccg ctccccggca tacctcacgt
     2341 cgccctgcgc gtcgcgcacc tgtgccgccc acgcgccgat ctcctggcgc agggtcaccg
     2401 gtgtggcgtc ctggaggtgg gtgcgcccca ccttgacgac gtcgcccagc tcgtcggcct
     2461 tcgcgtcgag ggccgcgcac aactcgtcga ccgccgggtg cagacgctcg atgagctcga
     2521 gcaccacggc gacgtgcacc gcggtcggga aggtgtcgtt gctcgactgg ctgcggttga
     2581 cgtcgtcgtt ggggtgcacg ggcgtcttcg acccgaggtc accgcccagg agctcgatgg
     2641 cccggttggc gatgacctcg ttggcgttca tgttgctctg ggttcccgac cccgtctgga
     2701 acacgacgag cgggaagtgg tcgtcgaggt ctccgcgggc cacctcgtcg gcggcctgca
     2761 ccacggcctc ggcgacgtcg gcgccgagca ggcccagctc ggcgttggcc agcgcagcgc
     2821 acttcttgag cagccccagc ccccgcacca ccggtcgccc ccacacgaag gtgtcacgcc
     2881 cgatgtcgaa gtgttcgagg ctgcgttgcg tctgtgcacc ccagtagcgg tcagcgggca
     2941 cctgaacggg ccccatggtg tcgcgctcgg tgcgggtctc cgtcatcacc ccacggtatg
     3001 cctcgcgagc cgcgtttccc cctgcccggg aatcattgcg ctccacccac cccgatcggg
     3061 gtgggtggag cgcaatgggt cggtggagct gtggtcagcg ggggtcggtc gggccctcgg
     3121 aggtcgggtc gtcgcgaacc acgtcgtcct ggatcacgct gtcctggata ccgctgtcct
     3181 gcggggcggc gtcggtgacg tcgctccaca cggcggcagg cgaggcgtcg gatgccgccg
     3241 gcccgccatc ggcggtgacg tcgccgtccg cggtggccgc ggcatccgtg gtgccagggg
     3301 cgctcgtggc cccggacgtg ctcggcatgc cggtgccctc gcgctgctcg gcgacccagt
     3361 cgacaccggt gagcacaccc gcacggacct tggcgaagga gtcgcggtac ttgccgtcgg
     3421 tcttctcgtc gaccttggca ccggccttgt cgagggtggc ctcgacgcgc tcgcggttct
     3481 cggcggcgta gtcgccggcg cgggtcgcag ccacgcgaag gaactggtcg gcctggtcgg
     3541 cgaaggtcgt gagcttcttg tcgagctcca tctccttggc cttggcctcg aggtcgcgca
     3601 acacggtgcg cagttggtcg gtggtggtca tgggggtccc ctttcgtgcg ggatcggccg
     3661 ggcgtcgaac accacggtcc atctgctcca actactgggc gcgtgaccgg tgttcccgcg
     3721 ggccgccgcc tcaggggtgg ctcagggtgc accctgagcc gggtggctgc tcgctcacaa
     3781 ccccgagcgc acaaccgccc catctgtcac actgcggttc tgccacctcg cagcgtcgcg
     3841 aacctggcca cggctgtcgc gacgcccgga ctgattggag cgtcgccgtg acccagcagc
     3901 acgaactccc cgcggtcccg ggcccggacg cgcaagaccc gcaggcggct gcggggggcc
     3961 agttcgtccc gaccgccgag ggtgccccca tgccgagcgc ctggcaggcc cggcgcgagt
     4021 ggcgcacggc cctcgagctg gcgagtgcgg cggtgcgcac gagagacctg cacgagtggc
     4081 cccgcgggga cgggcacccc gtcatcgtgc tccccggctt cctcgccggc cccgagtcca
     4141 cggccttcct gcgcagccac ctgcgccgac tggggtacca cgtccacgac tggcggcacg
     4201 gccgcaacct cggcgccagc ccggagctcg ccgaagagct cgaggacctc ctcctcgaga
     4261 tccacgaccg ctaccagcgc cgggtcagcc tcatcggctg gagcgccggg ggcatctacg
     4321 cccgcgagat ggcccgggcc ctgccggact acacccgctc ggtgatcacg ctcggcagcc
     4381 cgttccgcgg ccacccggca tccacccgcg cgtggcgcgt ctaccggctg atgaaccgcc
     4441 acaacctcga gaagatgttc accgacgagg ccctggccac ccgcgccgcg ccgctgagcg
     4501 tgccgacgac gtgcatctac tcgcgcaacg acggcatcgt cgcgtgggag tgctgcctgt
     4561 ccgaccccgc accgctgacg gagaacgtcg aggtcacggc cagccatctc ggctacggtc
     4621 acgagatgca cacgctgcgg gtcatctgcg accgactggc ccagcccgag ggggcatggc
     4681 tgccgtacac cggtcacgcg ccggccgtgc gcggctatgt gatggccatg gtgcggcgcc
     4741 ccgatct
//
LOCUS       DAOSOC010000180         4646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_18534_length_4646_cov_8.719015, whole genome shotgun sequence.
ACCESSION   DAOSOC010000180 DAOSOC010000000
VERSION     DAOSOC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18534_length_4646_cov_8.719015"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1785)
                     /gene="purF"
                     /locus_tag="PL014_11240"
     CDS             complement(187..1785)
                     /gene="purF"
                     /locus_tag="PL014_11240"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT57708.1"
                     /translation="MPRGDGRLSHDLIPGEKGPQDACGVFGVWAPGEDVAKLTYYGLY
                     ALQHRGQESAGIATSDGQRLLVYKDMGLVSQVFDERSLASLRGHLAVGHCRYSTTGGS
                     TWENAQPTLGGHAGGTVALAHNGNLTNSAELRDLVEGRLEGDAHRGELARGNTTDTAL
                     VTALLADHPDRTLEASALDVLPRLRGAFCFVFMDEHTLYAARDPHGIRPLVIGRLERG
                     WVVASETAALDIVGASYVREVEPGEMIIVDEDGLRSQRFAEPTPKGCVFEYVYLARPD
                     TTINGRVVHEARVEMGRALAREHPVEADLVMPTPESGTPAAIGYAQESGIPYGQGLVK
                     NSYVGRTFIAPSQTIRQLGIRLKLNPLKDVIKGKRLVVVDDSIVRGNTQRALVRMLRE
                     AGAAEVHVRISSPPVRWPCFYGIDFATRAELIATGLGVAEVCNSIGADSLGYISEEGM
                     IQATNQAPDRLCTACFTGSYPVPLPEEGLGKGVLELDLAVSTGANGRRVTGERHPHRH
                     VPVLDVEGVSTSAAGGAADAVRRP"
     gene            complement(1834..2073)
                     /locus_tag="PL014_11245"
     CDS             complement(1834..2073)
                     /locus_tag="PL014_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57709.1"
                     /translation="MTPARRPRFLPIIITGAVIGFLVGASLAVFGWFEDRDTMLESNY
                     APSAGIGYLGFMGALVFGLLAAVLAVLLDRRSERS"
     gene            2136..3770
                     /locus_tag="PL014_11250"
     CDS             2136..3770
                     /locus_tag="PL014_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT57710.1"
                     /translation="MRASENPSGSEPLDMTNPRMSQPETKPDGRPGRLWYAGGAALAA
                     AAAAGVGHLVAGLVSPEASPVLAVGSAVIDATPTPVKEWAVAQFGTADKPILIGSVAI
                     GALLLAGGIGLIARARRTLGLALLGALAFTSVGAALLRPTSTAVDLLPGFATALFGVL
                     AAGWFLGLLDQWGARDRDPAAGADAGATASGDGRSDRRRVLFAAAGLGAVAATGSTLG
                     KVLSRGASPSSVALPVAAEALPTLPGGLESTVRGISAFRTPNAQFYRIDTALVIPRVS
                     TDGWSLTIDGDVERPYTITYDELLAMPMVERDITLTCVSNEVGGPYIGAARWLGVRTK
                     DLLERAGVRPGADQILSHSTEGMTISTPVQALTDDREALVAVGMNGEPLPNRHGFPAR
                     LVTPGLYGFVGATKWLTRMEATTYAAQSAYWTDRGWATDAPILTQSRIDTPRGLDRRD
                     AGQVMIGGVAWAQQRGIAQVEVRVDDGPWQVATLGPDAGIDYWRQWYLVWDAAPGRHS
                     LSVRATDLTGELQPEGRTTPFPEGARGWHTIVVTID"
     gene            3924..4571
                     /locus_tag="PL014_11255"
     CDS             3924..4571
                     /locus_tag="PL014_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOT57711.1"
                     /translation="MRSTRLTVAALALALPLSIAACGSDSEMDAGSTDTMSSSSAPAP
                     SASASESMGTDMAGAPFGPGCAAVPADGAGSFGGMAKDPVATAASNNPVLSTLVTAVT
                     EAGLGDTLNTTPDITVFAPTNDAFAAMDKATLDKAMADPKGLLTTVLTNHVVAGRLAP
                     DQVAGEHETLAGTKITVEGSGEDFSVGEAKVVCGDVQTANATVYIIDGVLLPAAN"
BASE COUNT          672 a         1711 c         1616 g          647 t
ORIGIN      
        1 gctcggctcg gctcggctcg gctcagctcg gagagggccg accggtaggt accctccagc
       61 aatcgttgct ccggtcaagg gactccttgc gtcgcgcaag ggtccccaca gagaccccca
      121 tgcccccgct cacaggaaga gccgcgcccg aaacgtgccg gtcaggggcg gagcacggca
      181 cccaggtcag gggcggcgaa cggcatccgc ggcgccaccg gcggccgagg tcgacacgcc
      241 ctcgacgtcc agcacgggca cgtggcggtg cggatgccgt tcgcccgtca cccgcctacc
      301 gttcgccccg gtcgacaccg ccagatcgag ctcgagcacc cccttgccga gaccctcctc
      361 gggcaggggc accgggtacg acccggtgaa gcacgccgtg cagagccgat cgggcgcctg
      421 gttggtggcc tggatcatcc cctcctcgga gatgtagccc agcgagtcgg cgccgatcga
      481 gttgcacacc tcggccaccc cgagaccggt ggcgatcagc tcggcccggg tcgcgaagtc
      541 gatgccgtag aagcagggcc agcgaaccgg gggcgaggag atgcgcacgt gcacctcggc
      601 cgccccggcc tcacgcagca tccgcacgag ggcgcgctgg gtgttgccgc gcacgatcga
      661 gtcgtcgacg acgacgagcc gcttgccctt gatgacgtcc ttcaacgggt tgagcttgag
      721 ccggatgccc agttggcgga tggtctgcga cggcgcgatg aatgtgcgcc cgacgtagga
      781 gttcttcacc agcccctgcc cgtaggggat gccggactcc tgcgcgtacc cgatcgcggc
      841 cggcgtcccc gactccgggg tcggcatgac caggtcggcc tcgaccgggt gctcccgggc
      901 cagcgcgcgg cccatctcga ccctggcctc gtgcacgacg cggccgttga tcgtcgtgtc
      961 ggggcgtgcc aggtagacgt actcgaagac gcagcccttg ggggtcggct cggcgaagcg
     1021 ctgcgagcgc agcccgtcct cgtcgacgat gatcatctcg ccgggctcga cctcgcgcac
     1081 gtaggaggca cccacgatgt cgagggcggc cgtctccgag gcgacgaccc agccgcgttc
     1141 gagcctgccg atgacgaggg ggcggatgcc gtggggatca cgagcggcat acaacgtgtg
     1201 ttcgtccatg aagacgaagc agaacgcgcc gcgcagtcgg ggtagcacgt cgagggccga
     1261 ggcctcgagg gtgcggtcgg ggtggtcggc caggagtgcc gtgacgagcg cggtgtcggt
     1321 ggtgttgccc cgggccagct cgccccggtg ggcgtcgccc tcgaggcggc cctcgacgag
     1381 gtcgcgcagc tcggcggagt tcgtgaggtt gccgttgtgg gccagcgcca cggtgccacc
     1441 ggcgtggccg ccgagggtgg gctgggcgtt ctcccacgtc gagccgccgg tggtggagta
     1501 gcggcagtgg cccactgcga ggtggccgcg cagcgacgcc agcgagcgct cgtcgaagac
     1561 ctgggagacc aggcccatgt ccttgtagac cagcagccgc tgcccgtcgg acgtcgcgat
     1621 gccggccgac tcctgcccgc ggtgctggag ggcatagagg ccgtagtagg tcagcttggc
     1681 gacgtcctca ccgggggccc agaccccgaa gaccccgcag gcatcctgcg ggcccttctc
     1741 accgggaatc aggtcgtggc tgaggcgtcc gtctccgcgt ggcacgtgcc cgatcctccc
     1801 acaccctgct ggtggcaccc caccgacgaa cggtcaggag cgttcgctgc gccgatcgag
     1861 cagcacggcg agcacggccg cgagcagacc gaacaccagg gcgcccatga agcccaggta
     1921 gccgatgccg gccgacgggg cgtagttcga ctccagcatc gtgtcgcggt cctcgaacca
     1981 gccgaagacg gccagcgagg cccccacgag gaacccgatg acggccccgg tgatgatgat
     2041 gggcaggaag cgcgggcggc gtgcaggtgt cacggtccct aaggtacgtg aggccgagca
     2101 tccctcgacg aaccaccctc gcgcacactt gggtcgtgcg cgcatccgag aacccctccg
     2161 gttccgaacc actggacatg acgaaccctc ggatgtcgca gccagagacc aagcctgacg
     2221 ggcgcccggg gcgcctctgg tacgccgggg gcgccgcact ggccgcggcc gccgccgccg
     2281 gggtgggtca cctcgtcgcc gggctggtct cccccgaggc ctcacccgtg ctcgccgtgg
     2341 ggtccgccgt catcgacgcc actcccaccc cggtcaagga atgggcggtg gcgcagttcg
     2401 gcaccgctga caagcccatc ctcatcggat cggtggcgat cggtgcgctg ctgctggccg
     2461 gcggcatcgg gctgatcgcc cgcgcccggc gcaccctggg cctggccctg ctcggggccc
     2521 tggccttcac gtccgtcggc gccgccctgc tgcgccccac gagcaccgcg gtggacctgc
     2581 tgcccgggtt cgccacggcg ctgttcggag tgctggccgc cggctggttc ctcgggctcc
     2641 tcgaccagtg gggggcgcgt gatcgcgacc cggcggcggg ggcggatgcc ggcgccacgg
     2701 catccggcga cggccgcagc gatcgtcggc gcgtgctgtt cgccgcggcc gggctgggag
     2761 cggtcgccgc caccggcagc accctgggca aggtgctgtc acgcggcgca tcgccgtcgt
     2821 ccgttgccct gccggtggcc gccgaggccc tgccgacgct gccgggtggc ctggagtcga
     2881 ccgttcgcgg catctcggcc tttcgcaccc cgaacgcgca gttctaccgg atcgacacgg
     2941 ccctggtcat cccccgggtc tccaccgacg gctggtccct gaccatcgac ggcgacgtgg
     3001 agcgtcccta cacgatcacc tacgacgagc tcctcgcgat gccgatggtc gagcgcgaca
     3061 tcaccttgac ctgcgtgtcc aacgaggtcg gcggcccgta catcggggcc gcccggtggc
     3121 tcggcgtgcg caccaaggac ctgctcgagc gggcgggcgt gcggccggga gccgaccaga
     3181 tcctcagcca ctccaccgag ggcatgacga tctcgacccc cgtgcaggcc ctgaccgacg
     3241 accgcgaggc cctcgtggcc gtcgggatga acggtgagcc gctgcccaac cgccacggct
     3301 tcccggcgcg gctggtcacc ccggggctgt acggcttcgt cggtgccacc aagtggctga
     3361 cccgcatgga ggcgacgacc tacgccgcgc agagcgccta ctggaccgac cgcgggtggg
     3421 ccaccgacgc accgatcctc acccagtcac gcatcgacac ccctcgcggg ctggaccggc
     3481 gtgatgcggg gcaggtcatg atcggtgggg tggcgtgggc gcagcagcgc gggatcgccc
     3541 aggtcgaggt gcgcgtcgac gacgggccgt ggcaggtcgc caccctcggg ccggatgccg
     3601 gcatcgacta ctggcgccag tggtacctgg tgtgggatgc cgccccgggt cgccactcgc
     3661 tgagcgtgcg cgcgaccgac ctgaccggcg agctgcagcc cgagggccgc acgacgccgt
     3721 tccccgaggg tgcccggggc tggcacacca tcgtcgtcac catcgactga caccctctgc
     3781 ccctcccaca acgtccggca ccttggtggc tccagccgcc aaggtgccgg acgttgttct
     3841 ggtccgggca atccggttca cgagctgctc cgaaccactg gtgtacgcac cacgacacac
     3901 acctgcgaga aggagcacct cccatgcgtt ccacccgact gaccgttgca gccctggccc
     3961 tggccctccc cctgtcgatc gccgcctgcg gctcggacag cgagatggat gccggctcca
     4021 ccgacaccat gagctcgtcg tccgctccgg ccccctcggc ctccgcctcg gagtcgatgg
     4081 gcaccgacat ggctggcgcc ccgttcggcc ccggctgcgc ggccgtcccc gccgacggtg
     4141 cgggctcgtt cggcggcatg gccaaggacc cggtcgccac ggcggcgagc aacaaccccg
     4201 tcctgtcgac gctggtcacc gcggtgaccg aggccggcct cggtgacacc ctcaacacca
     4261 cgccggacat caccgtcttc gcgccgacca acgacgcctt cgcggccatg gacaaggcca
     4321 ccctcgacaa ggccatggcc gacccgaagg gcctgctcac cacggtgctc accaaccacg
     4381 tcgtcgcggg ccgcctcgcg cccgaccagg tcgccggtga gcacgagacc ctggccggca
     4441 ccaagatcac ggtcgagggc tccggagagg acttcagcgt cggcgaggcc aaggtcgtct
     4501 gcggtgacgt gcagaccgcc aacgccaccg tctacatcat cgacggcgtg ctgctccccg
     4561 cagccaactg acgtctgacc gacctccaca gtccatgatg ggtgacatga cagcctcctc
     4621 ctcgggtacc tccgtgcccg gggagg
//
LOCUS       DAOSOC010000181         4512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_19310_length_4512_cov_9.778999, whole genome shotgun sequence.
ACCESSION   DAOSOC010000181 DAOSOC010000000
VERSION     DAOSOC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19310_length_4512_cov_9.778999"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PL014_11260"
     CDS             complement(<1..278)
                     /locus_tag="PL014_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOT57712.1"
                     /translation="MDTSPRPVPRSRRQRAPRHGGSRWGARPALVLALAGTLLATTGA
                     VVSAASDEPDLMTMKLRIEGGYYTEKALSQDSDGDGWTDWVERLNGTDP"
     gene            345..1031
                     /locus_tag="PL014_11265"
     CDS             345..1031
                     /locus_tag="PL014_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOT57713.1"
                     /translation="MSTTPGAPTNPAGADGIPIDALDFYDDLAVENTRTWWQANKDRY
                     LRSVRAPLDALLAALEDEFGAAHVYRPNRDVRFSADNSPYKDHQGALAATVPGMGFYV
                     QVSAAGLMTGGGFYPTGTDQVSRLRAAIDSPRSGQDLQRITDALVGQGFELGGERVAT
                     RPRGVAADHPRLPLMRFKSLVAKREHGCPPWLSTPEVVTQVRADWQSVRPLVDWLTEH
                     VGATDHPRRR"
     gene            1086..1511
                     /locus_tag="PL014_11270"
     CDS             1086..1511
                     /locus_tag="PL014_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOT57714.1"
                     /translation="MDSGDSDRKTHPTDADVTAFLDAVPDPRRRADALDALDLIREVT
                     GAEPRMWGPTMVGFGRQPYRTADGKEHEWFAVGLSPRKAALTLYGLTFYDSNTDLLDR
                     LGPHTTGKGCVYVKKFEALDREVLTDLVRRAWETNHQPD"
     gene            1528..4362
                     /locus_tag="PL014_11275"
     CDS             1528..4362
                     /locus_tag="PL014_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HOT57715.1"
                     /translation="MTLTDRLNRARTRSDAGAPTAPSGSTARGGSSGSSASDAAFDVF
                     IEWAGDQGLTLYPAQEEAVLALAGGAHVVLATPTGSGKSLVAVAAHAQALAQGQRSWY
                     TAPIKALVSEKFFALVAIFGAEQVGMLTGDAAVNPGAPIICATAEVLANHALRDRELA
                     DIGLVVMDEFHFYTEPDRGWAWQVPLLLLPDTQFLLMSATLGDTRPFEEDLTRRTGRE
                     TIAVTGVERPVPLEFEWVLTPVHETLELLLRDDRGPVYVVSFTQSSAVEQAQALMSVD
                     IVSSARKAAIKEAVGGFRFGKGFGQALRRLVLHGIGVHHAGMLPKYRRLVETLAQRGL
                     LAVICGTDTLGVGINVPIRTVLLTSLTKYDGRKQRVLRAREFHQIAGRAGRAGFDSVG
                     YVVVQAPEHVVLNAKALAKAGDDAKKRRKVVHKKAPEGTLTYTEATYERLVMAQPEPL
                     VSKMRVNHAMVLNVLDQWEGQQAALHTLLLDNHEEPARRDALVERAVQVLTSLLRAGV
                     VEPDDGSSLEDWLPAEVEATTSDAVAPQPLPRLLPDVVADEGDLGPIGELLAAAGHLA
                     PAPSAPVAQADETPQEAAEERQPAGKVEQFIRGEGRRYDEGPFQVALDLHDGFALNQP
                     LSAFALATLEMFDPDAPGYALDVVSVIEATLDDPRPVLMAQRFEARGEAVGAMKAEGM
                     EYEERMDALDDVTWPRPLAEELEGALRVYRQRHPWVDPRDLSPKSVVREMYERAMTFG
                     EFIAHHKLFRAEGVVLRYLTDAYRALRSTVPTSARTEELDDIVEWLGEVVRHTDSSLL
                     DEWEALANPGDDPLTEVRTPTEGRALSANPRALRVMVRQSMFRRVELLSLRRYAALAA
                     LGGGLSDAAWSAAHEAYLDDYDDFGTGPQARGPALLRIDDSDAEVWLVEQILDDPEGD
                     HDWRISAELDVAATDEAGELVLTVTGLGPT"
BASE COUNT          671 a         1649 c         1580 g          612 t
ORIGIN      
        1 gggtcggtgc cgttcagccg ctcgacccag tcggtccaac cgtcaccgtc ggagtcctga
       61 ctgagtgcct tctcggtgta gtagccgccc tcgatgcgca gcttcatggt catcaggtcg
      121 ggctcgtcgg atgccgcgct caccacggct cccgtcgtgg cgagcaaggt gccggcgagg
      181 gcaagcacga gggcggggcg cgccccccag cggctcccgc cgtgacgggg cgcacgctgg
      241 cgccgggacc ggggcacggg gcgaggtgag gtgtccatgt cgaggaggag cggcgtccgg
      301 ccgtcctgat accgcggcca gcgactcgtg gcagggtgtc gacggtgagc acgacacctg
      361 gtgcgccgac gaacccggcc ggcgcggacg gcatcccgat cgacgccctg gacttctacg
      421 acgacctcgc cgtcgagaac acccgcacgt ggtggcaggc caacaaggac cggtacctgc
      481 ggtcggtgcg tgcaccactg gacgccctgc tcgcggcgct ggaggacgag ttcggggcag
      541 cgcacgtgta ccgccccaac cgcgacgtgc ggttcagcgc ggacaattcg ccgtacaagg
      601 accaccaggg agcgctcgcc gcgacggttc ccgggatggg gttctacgtg caggtcagcg
      661 ccgccgggct gatgaccggc ggcggtttct accccacggg caccgaccag gtgtcgcgac
      721 tgcgggcggc gatcgacagc ccgcgctcgg ggcaggacct gcaacggatc accgacgcgc
      781 tcgtggggca ggggttcgag ctcggcgggg agcgcgtggc cacccggccg cggggggtcg
      841 ccgcggatca cccccggctg ccgttgatgc gcttcaagag cctggtggcc aagcgggagc
      901 acggctgccc gccgtggctg tcgacccccg aggtcgtcac gcaggtccgc gcggactggc
      961 agagcgtgcg gccgctcgtc gactggctga ccgagcacgt gggcgcgacg gatcaccccc
     1021 gccgccgctg acccccgggc ggtggccgac ggcatccggg cggccgctgg aggaggatgt
     1081 cggccatgga ctccggtgac agcgaccgca agacccaccc cacggacgcc gacgtcacgg
     1141 cattcctgga tgccgtgccc gacccgcgcc gccgagccga cgccctcgac gccctggatc
     1201 tcatccgcga ggtgaccggt gccgagccgc ggatgtgggg gccgacgatg gtcggcttcg
     1261 gccgccagcc gtaccgcacg gccgacggca aggagcacga gtggttcgcc gtcgggctct
     1321 ccccccgcaa ggccgcgctg accctgtacg ggctgacctt ctatgacagc aacaccgacc
     1381 tgctcgaccg cctcgggccg cacaccaccg gcaagggctg cgtctacgtc aagaagttcg
     1441 aggccctcga ccgtgaggtg ctgaccgacc tcgtccgccg cgcctgggag accaaccacc
     1501 agcccgactg agggagactg gggcgcgatg accctcaccg accgactgaa ccgcgcccga
     1561 accaggtcgg atgccggtgc ccccaccgcg cccagcggct ccaccgcgcg cggggggtcc
     1621 agcgggtcct ccgcgtcgga tgccgccttc gacgtcttca tcgagtgggc cggcgaccag
     1681 ggcctgaccc tgtaccccgc gcaggaggag gcggtgctgg ccctggccgg tggagcccac
     1741 gtcgtgctcg cgaccccgac cggctcgggc aagtcgctcg tggccgtggc cgcgcacgcg
     1801 caggccctcg cgcaggggca gcgcagctgg tacaccgcgc cgatcaaggc cctggtcagc
     1861 gagaagttct tcgcgctcgt cgcgatcttc ggggccgagc aggtcggtat gctcaccggc
     1921 gacgccgcgg tgaacccggg cgccccgatc atctgcgcca ccgccgaggt cctggccaac
     1981 cacgctttgc gtgacaggga actggccgac atcggcctcg tcgtcatgga cgagttccac
     2041 ttctacaccg agcccgaccg cggctgggcc tggcaggtgc cgctgctgct cctgcccgac
     2101 acccagttcc tcctcatgtc ggcgaccctg ggcgacaccc gcccgttcga ggaggacctc
     2161 acccgccgta ccggccgcga gaccatcgcc gtcaccgggg tcgagcgccc cgtgccgctg
     2221 gagttcgagt gggtgctcac accggtccac gagaccctcg agctgctgct gcgcgacgac
     2281 cgcggcccgg tctacgtcgt gtcgttcacc cagtcctcgg ccgtcgagca ggcgcaggcg
     2341 ctgatgagcg tcgacatcgt ctcctcggcc cgcaaggccg ccatcaagga ggccgtcggc
     2401 gggttccggt tcggcaaggg gttcggccag gcgctgcgcc ggctcgtgct gcacggcatc
     2461 ggcgtccacc acgcggggat gctgcccaag taccgccgcc tcgtcgagac cctggcccag
     2521 cgcgggctgc tcgcggtcat ctgcggcacc gacaccctcg gcgtcggcat caacgtgccg
     2581 atccgcacgg tgctgctcac ctcgctcacc aagtacgacg ggcgcaagca gcgcgtgctg
     2641 cgggcccgtg agttccacca gatcgcgggc cgggccgggc gggccgggtt cgactccgtc
     2701 gggtacgtcg tcgttcaggc ccctgagcac gtcgtgctca acgccaaggc cctggccaag
     2761 gccggcgacg acgcgaagaa gcgccgcaag gtcgtgcaca agaaggcccc ggagggcacg
     2821 ctcacctaca ccgaggccac ctacgagcgc ctcgtcatgg cccaacccga gccgttggtc
     2881 tccaagatgc gggtcaacca cgcgatggtg ctcaacgtgc tcgaccagtg ggagggccag
     2941 caggcagcgc tgcacaccct cctgctcgac aatcacgagg agcccgcccg tcgcgacgcc
     3001 ctcgtcgagc gcgcggtgca ggtgctgacc tcgctcctgc gcgccggggt ggtcgagccc
     3061 gacgacggct cctccctcga ggactggctc cccgccgagg tcgaggcaac gacgtcggat
     3121 gccgttgcgc cacagccgct cccacgtctg cttcccgatg tggtcgctga cgagggtgac
     3181 ctcggcccga tcggcgaact gctcgccgcc gccggccacc tcgcccctgc cccttctgcc
     3241 cccgtggccc aggctgacga gacgccgcag gaggctgccg aggagcggca accggcagga
     3301 aaagtcgagc agttcatccg cggcgaggga cggcgctacg acgaagggcc cttccaggtc
     3361 gcgctcgacc tgcacgacgg cttcgcgctc aaccagccgc tgtcggcgtt cgccctggcc
     3421 accctcgaga tgttcgaccc cgatgccccc ggctacgcgc tcgacgtcgt gagcgtcatc
     3481 gaggccacgc tcgacgaccc gcgcccggtg ctcatggcgc agcggttcga ggcccgcggc
     3541 gaggccgtcg gcgcgatgaa ggccgagggc atggagtacg aggagcggat ggacgccctc
     3601 gacgacgtca cctggccgcg cccgctcgcc gaggagctcg agggggcgct gcgggtctac
     3661 cggcagcggc acccgtgggt cgacccgcgc gacctgtcgc cgaagtcggt cgtgcgcgag
     3721 atgtacgagc gggcgatgac cttcggcgag ttcatcgcgc accacaagct cttccgggcc
     3781 gagggggtcg tgctgcgcta cctcaccgat gcctaccggg cgctgcgctc gaccgtgccg
     3841 acgtcggcgc gcaccgagga gctcgacgac atcgtcgagt ggctcggcga ggtcgtgcgg
     3901 cacaccgact ccagcctgct cgatgaatgg gaggccctgg ccaaccccgg cgatgacccg
     3961 ctcaccgagg tgcgcacccc caccgagggc cgcgccctgt cggcgaaccc tcgggcactg
     4021 cgagtcatgg tgcggcagag catgttccgc cgggtcgagc tgttgtcgct tcgtcggtac
     4081 gccgctctgg cggccctcgg cggcgggctc tcggatgccg cgtggtcggc tgcgcacgag
     4141 gcctacctcg acgactacga cgacttcggc accggcccgc aggctcgggg gccggccctg
     4201 ctgcggatcg acgactcgga tgccgaggtg tggctggtcg agcagatcct cgacgacccc
     4261 gagggcgacc acgactggcg gatcagcgcc gagctcgacg tggcggccac cgacgaggca
     4321 ggcgaactcg tgctgaccgt caccggcctg ggccccacct gagcacgccg aaaccgcacg
     4381 atctgaaggc aaaacttctc cgccatcaca gggatgacgg agaagatccg cccgggcgga
     4441 gatatatctc cgccagagcc aaacaacccc gacgtcactg ggatggcgga gaagtttcag
     4501 ggggtgccga gg
//
LOCUS       DAOSOC010000182         4506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_19335_length_4506_cov_10.248259, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000182 DAOSOC010000000
VERSION     DAOSOC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19335_length_4506_cov_10.248259"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PL014_11280"
     CDS             <1..386
                     /locus_tag="PL014_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT57716.1"
                     /translation="TDPSDEMFTTEYFGPILAVHVYPDRQYAKVLEQMESVAPYALTG
                     AIIAQDRAVVADALHRLRFAAGNFYINDKPTGAVVGQQPFGGGRASGTNDKAGSAANL
                     LRWTSVRSIKENFVPPTQHTYPHMG"
     gene            complement(460..957)
                     /locus_tag="PL014_11285"
     CDS             complement(460..957)
                     /locus_tag="PL014_11285"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HOT57717.1"
                     /translation="MRVTTREQLDGTVEEVYALFTDVAFQEAKCLATTDDANHFSVDV
                     MGGPTGEKVRTERHLPSETLPDVAKSFVGEHLTIIEVQTWSMPAADGSRESVVDMHVK
                     GAPLTLKGTLRLEPSKGGTVQVLDAELKAHVPFIGGKIEQAAADPIRTAIGIETGLLR
                     EWLAR"
     gene            complement(994..1515)
                     /locus_tag="PL014_11290"
     CDS             complement(994..1515)
                     /locus_tag="PL014_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57718.1"
                     /translation="MSVVPGDPASLSACASTARSVGHGLTGQASRVRATSRELAQGWT
                     GRTSAGVRQRASGLADATDAAASELDRMGRVLQDHASDLADLVARGRAVEERARAAGL
                     VVRDGRVELAWGVTGAADSGADREREHLRTALQADLDLVLAQHRRRRDWVLEVLRDST
                     TALAGVSHKLRGG"
     gene            complement(1512..1817)
                     /locus_tag="PL014_11295"
     CDS             complement(1512..1817)
                     /locus_tag="PL014_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57719.1"
                     /translation="MPLGPDPALARAAADALFALRDRVADAEDDVLSSLVVTGEPRPQ
                     RVLDDWLDQVADTLRAVGETASDLAVSLNARADVAADTPATAPSPTVRTAAVDGRRA"
     gene            1897..3129
                     /locus_tag="PL014_11300"
     CDS             1897..3129
                     /locus_tag="PL014_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT57720.1"
                     /translation="MASDDAPRGTTASDAALNTASDAVRPDAAVESEAVELTAALVAI
                     DSVNPGLVPGAAGEAAIVEHLRHRLSRGGFDTHVVQAPGHVGRPSLVAVSPAGAPGPT
                     VVLTGHLDTVGVEGMDDPFGARVDGDRLEGRGACDMKGGVAAMVVAAEELARRRLPGR
                     VVLALVADEEDASLGTLAVLDALPGLGIEPDVAVIGEPTWLALAQSLRGYALVEVTLE
                     GRAAHSSQPEQGVNAVAHLGRLLAAVESRGSLVAERGGSLMVTVASGGESPFVLARHA
                     TAIVERRTVPGERSEAALAEVEHLLVHLRLQDDTVSARARLVVARDPWQLDDSGPAAA
                     LAADLSRALGDDAASEPFHAPYWMEAPLWQAAGIPTVVCGPAGGGLHAADEWVDLHQV
                     RRYTLALIEAATTWGASA"
     gene            3126..4313
                     /locus_tag="PL014_11305"
     CDS             3126..4313
                     /locus_tag="PL014_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT57721.1"
                     /translation="MTSFARRAERVQPSAIREFLALAGQPGITSFAGGYPDPTLFPMR
                     ELHGIYDALLTDGNASALQYTASEGLPELRALVAARLSADGMPCTADDVLITQGGQQG
                     LDLTAKLFVDAGDVIVTERPTFLGALIAFNPCEPDYRSVPMDDEGMDTDALEQVLRTT
                     DRVKLVYTVPDHQNPTGRTMSLARRRRLVELAEEFDVVVLEDTPYRELRYEGERLPTI
                     KSLDTTGRVVHLGSFSKILAPGLRLGWALAEPEVREKLALLKLAADTQNGTLNMRAAA
                     AYLGGYDIEGHIAGMLPTYRHQRDLMLASLQEHFPPGITWTRANGGLFTWVTFPAGLD
                     MAAFQRDVLIPQAGVIVVPGTPFFADRPEANHARMSYSGVPDDRLVEGVRRMGALLTD
                     ALR"
BASE COUNT          599 a         1703 c         1578 g          626 t
ORIGIN      
        1 gcaccgaccc gagcgacgag atgttcacca ccgagtactt cgggccgatc ctcgcggtgc
       61 acgtctaccc ggaccggcag tacgccaagg tgctggagca gatggaatcg gtcgcgccct
      121 acgccctcac cggcgcgatc atcgcccagg accgcgccgt cgtcgccgac gccctgcacc
      181 gactgcgctt cgcggcgggc aacttctaca tcaacgacaa gccgacgggt gcggtcgtcg
      241 gacagcagcc gttcggtgga ggtcgcgcct cgggaaccaa cgacaaggcc ggctcggccg
      301 cgaacctcct gcgctggacg tcggtgcgct cgatcaagga gaacttcgtg ccgccgaccc
      361 agcacaccta cccccacatg ggctgaccac tccgactcat tgcactccag ccactctcag
      421 ggggtggctg gagtgcaatg agtcgggaga ggggagggct cagcgggcga gccactcccg
      481 cagcagcccg gtctcgatcc cgatcgccgt ccggatcggg tccgcggcgg cctgctcgat
      541 cttcccgccg atgaacggca cgtgcgcctt gagctcggcg tcgagcacct gcaccgtccc
      601 gcccttcgag ggctccaggc gtagcgtgcc cttgagcgtc aggggcgcgc ccttgacgtg
      661 catgtcgacc acggactcgc gcgagccgtc cgcggccggc atcgaccacg tctggacctc
      721 gatgatcgtc aggtgctcgc cgacgaacga cttcgcgacg tcgggcaggg tctccgacgg
      781 caggtgacgc tcggtgcgca ccttctcgcc cgtcgggccc cccatgacgt cgacgctgaa
      841 gtggttcgcg tcgtcggtcg tcgccaggca cttggcctcc tggaaggcca cgtccgtgaa
      901 gagggcgtag acctcctcga cggtcccgtc cagctgctcg cgcgtcgtca ctcgcaccct
      961 gtcatcctct cggtcggcgg ttcggcgatc ctctcatcct ccccgcagct tgtgggagac
     1021 cccggccagg gccgtcgtcg agtcgcgcag cacctcgagc acccagtcgc gccgccggcg
     1081 gtgctgcgcg agcacgaggt cgaggtcggc ctggagcgcc gtgcggaggt gctcgcgctc
     1141 gcggtcggcc ccggagtcgg cggcccccgt cacgccccac gccagctcga cccgtccgtc
     1201 gcgcaccacg agtcccgcgg cgcgggcccg ctcctcgacg gcgcgacccc gggccacgag
     1261 gtcggccagg tcgctggcgt ggtcctggag cacccgcccc atccggtcga gttcgctcgc
     1321 tgccgcgtcg gtcgcgtcgg cgagaccgct ggcgcgctgc cgcaccccgg ccgaggtccg
     1381 gccggtccac ccctgggcga gctctcgcga cgtggcacgc acccgactcg cctgcccggt
     1441 caggccgtgt ccgaccgagc gcgccgtcga ggcgcacgct gacagggatg ccgggtcgcc
     1501 cggcaccacg ctcacgctcg cctcccgtcg acagcagcgg tccgcaccgt cggtgacggg
     1561 gcggtggcgg gggtgtccgc cgcaacgtcg gcgcgagcgt tcagggacac ggcgaggtcg
     1621 gatgccgtct cgcccaccgc tcgcagggtg tcggccacct ggtccagcca gtcgtcgagc
     1681 acccgctgcg ggcgtggttc ccccgtgacg accagtgagg agaggacgtc gtcctcggcg
     1741 tcggcgacgc ggtcgcgcag ggcgaacagg gcatcggcgg ccgcgcgggc gagcgccggg
     1801 tcgggaccaa ggggcacccc ttcactgtag gcggcgcgac gcccactcct cggagttacc
     1861 cacagggccc gcggcggggg tcctaggctc tggcccatgg ccagcgacga cgctcctcga
     1921 gggaccacgg catccgatgc cgccctgaac acggcatccg acgccgtgcg acccgacgcc
     1981 gccgtagagt ccgaggccgt cgagctgacg gccgcactcg tggcgatcga ctcggtgaac
     2041 ccggggctcg tgccgggggc tgcgggggaa gcagccatcg tcgagcacct gcgacaccgg
     2101 ctgtcacgcg gcggcttcga cacccacgtg gttcaggctc ccgggcacgt gggccgaccg
     2161 agcctcgtgg cggtcagccc ggccggcgca cccgggccca ccgtcgtcct gaccggccac
     2221 ctcgacaccg tcggggtcga gggcatggac gatccgttcg gggcgcgggt cgacggcgac
     2281 cgtctcgagg ggcggggtgc ctgcgacatg aagggcgggg tggccgcgat ggtcgtcgcc
     2341 gccgaggaac tcgcccgccg ccggctgccc ggtcgggtcg tgctcgccct ggtggccgac
     2401 gaggaggacg ccagcctggg caccctggcc gtgctcgatg cactccctgg tctcggcatc
     2461 gagcccgacg tcgccgtcat cggtgagccc acgtggctcg cgctggcgca gtccctgcgg
     2521 ggctacgccc tcgtcgaggt gactctcgag gggcgcgccg cgcactcgtc gcagcccgag
     2581 cagggtgtca acgctgtcgc ccacctcggc cgcctgctcg ccgccgtgga gtcccgcggg
     2641 agcctggtcg ccgaacgcgg cggctcgctc atggtcaccg tggcttccgg cggggagtcg
     2701 ccgttcgtgc tggctcgaca cgccacggcg atcgtggagc ggaggaccgt gccgggcgag
     2761 cgctccgagg cggccctcgc cgaggtcgag cacctgctcg tgcacctgcg cctccaggac
     2821 gacaccgtca gcgcccgcgc gcggctcgtc gtcgcgcgcg acccgtggca gctcgacgac
     2881 tccggcccgg cggccgccct cgcggccgac ctgagccgcg cgctcggtga cgacgcggca
     2941 tccgaaccct tccacgcgcc ctactggatg gaggcgccgc tgtggcaggc cgccggcatc
     3001 cccacggtgg tctgcggccc ggccggtggt gggctgcacg ccgccgacga gtgggtggac
     3061 ctgcaccagg tgcgccgcta caccctcgcc ctcatcgagg cggccacgac gtggggggca
     3121 tcggcatgac gtccttcgcc cgccgcgccg agcgcgtcca gccctcggcg atccgcgagt
     3181 tcctggccct ggccggccag cccggcatca cgtccttcgc cggcggctac cccgacccga
     3241 cgctgttccc gatgcgcgag ctgcacggca tctacgacgc cctcctcacc gacggcaacg
     3301 cgtcagcgct gcagtacacg gcatccgaag ggttgcccga gctgcgcgcc ctcgtcgccg
     3361 cccgcctgag tgcggacggg atgccgtgca cggcggacga cgtcctcatc acccagggtg
     3421 ggcagcaggg tctggacctc accgccaagc tcttcgtgga tgccggcgac gtcatcgtca
     3481 cggagcggcc caccttcctc ggggcgctga tcgccttcaa cccgtgtgag cccgactacc
     3541 ggtcggtgcc gatggacgac gagggcatgg acaccgacgc cctcgaacag gtgctgcgca
     3601 cgaccgatcg ggtcaagctc gtctacaccg tgcccgacca ccagaacccg acgggccgca
     3661 cgatgagcct ggctcggcgc cggcggttgg tcgagctggc ggaggagttc gacgtcgtcg
     3721 tcctcgagga caccccctac cgcgaactgc ggtacgaggg ggagcggctg ccgacgatca
     3781 agagcctgga caccaccggc cgggtcgtgc acctcggcag cttctcgaag atcctcgcgc
     3841 ccgggctgcg cctcggctgg gcgctcgcgg agcctgaggt ccgcgagaag ctcgcgctcc
     3901 tcaagctcgc cgcggacacc cagaacggca ccctcaacat gcgggccgcg gccgcctacc
     3961 tcggtgggta cgacatcgag ggccacatcg cggggatgct gccgacctac cggcaccagc
     4021 gcgacctcat gctcgcctcc ctgcaagagc acttcccgcc cggcatcacc tggacccgcg
     4081 ccaacggcgg gttgttcacc tgggtgacct tccccgccgg gctggacatg gccgcgttcc
     4141 agcgcgacgt gctgatcccg caggccggcg tcatcgtcgt gcccggcacc ccgttcttcg
     4201 ccgaccggcc cgaggccaac cacgcccgga tgagctactc cggggtgccg gacgaccgct
     4261 tggtcgaggg agtgcgccgc atgggggcgc tgctcaccga cgcgctgcgc tgagcgaggc
     4321 cagtgcctgt cgagtgagcg caacatgccg tgttgctcga cgcggcacgg cgtgttgcgc
     4381 tcactcgatc gggcgtgacc tgggccagat ggtgctcgcc cggtggtgcg ggcgatccgc
     4441 gcgaggaacg ggggcagact gaggcccatg cccaccgatc cccgaggcct gcgcggcgac
     4501 gcgccc
//
LOCUS       DAOSOC010000183         4442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_19697_length_4442_cov_10.435377, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000183 DAOSOC010000000
VERSION     DAOSOC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19697_length_4442_cov_10.435377"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PL014_11310"
     CDS             <1..653
                     /locus_tag="PL014_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT57722.1"
                     /translation="IVKGLGSERAWVLLGTESLQTFGGSGFLQDYPIEQYVRDAKIDT
                     LYEGTTAIQGLDFFFRKIVRDKGQALTFLSSQMQEFAKDLGAHDGRLDRDRELLGQGL
                     EDVQGILGFMVGELMKSDPRNGGEITNIYSVGQNTSRLLLSAGDLVVGWLLLRQAAVA
                     LDALAAGATGKDKDFYEGKVAAAQFYARQVLPRLSAERAMAEATDSMLMDVPESAF"
     gene            complement(810..1058)
                     /locus_tag="PL014_11315"
     CDS             complement(810..1058)
                     /locus_tag="PL014_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HOT57723.1"
                     /translation="MYIGLGIVLFVLGAILAFALNIDIPGVSDDTLGYILMGAGVVAI
                     LLSFLATRRTAAGYSATRESHVDPATGSRIDRTDVDPR"
     assembly_gap    1319..1418
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1573..2955)
                     /locus_tag="PL014_11320"
     CDS             complement(1573..2955)
                     /locus_tag="PL014_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT57724.1"
                     /translation="MDARDAVERAVAELVASLPSGVVVTDPDAAEKYRFDWTHDARAG
                     HPAAVVRAENAGHVVTTMRWATRHGIPVVPRGAGSGLSGGALAVDGGIVLSTERMRAV
                     EIDVDARVAVVEPGALNVEVKRAAAEHGLWYPPDPSSFEMCSIGGNIATNAGGLCCVK
                     YGVTTDYVLGLDVVLADGTPVTLGGKRVKDVAGLSLLKLFVGSEGTLGVVTRAILRLV
                     PGQQPPATVVATFPSVAAAADAVIALGRTVRASMVELMDHGSINAVEDHTPMGLNRDA
                     GALLLIQSDAPSAARADEVAAAVAACEGAGASEVFATDDPAEGELFVRARRLHFTAIE
                     ARGNVLPEDVGVPVPQLPALLAAVAEIAARHDLEIPMVAHAGDGNTHPAIVYPAGDAD
                     AERRARLAFDDIMAAAIALGGTITGEHGVGRLKAGSLEAQLGPDVMALNRRVKAALDP
                     HGILNPGSFV"
     gene            complement(3234..4073)
                     /locus_tag="PL014_11325"
     CDS             complement(3234..4073)
                     /locus_tag="PL014_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HOT57725.1"
                     /translation="MRIAVVQLAYGDEESFEQRVERVAGLVAAQAGHDLVVLPELWGA
                     TGFDYSRWPGAAQPLDGPWARAMADAARTAGVVLHAGSFVERLPEAGVDGNDLANTSL
                     LFAADGQVIASYRKIHRFGFGSGEPKLMEAGDEVVVTGIPLRDGGSVTAGLSTCYDLR
                     FPELYRRQVAAGAELFVIPAAWPAARVAHWTLLGRARAVENQAVVVQCNTAGSHGGYE
                     MGGHSQVVSATGAVLAEAGGDERRSGGDGERAVECVLSVDVDLAVITEYREAFPVLGD
                     RRL"
     gene            complement(4097..>4442)
                     /locus_tag="PL014_11330"
     CDS             complement(4097..>4442)
                     /locus_tag="PL014_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOT57726.1"
                     /translation="PLMLLVILFATISFHAFFGVALTGSDTLLAPDFFTQLNLPWGPD
                     PLADQQRAGEIAWGVGEAPTLVLAVIVAVQWFRRDEAESRRLDRQADRDGDAQLRAYN
                     EHLAQLRGETER"
BASE COUNT          636 a         1569 c         1547 g          590 t
ORIGIN      
        1 cgatcgtcaa gggcctgggc tccgagcgcg cctgggtgct gctcggcacc gagtcgctgc
       61 agaccttcgg cgggtccggg ttcctccagg actacccgat cgagcagtac gtgcgtgacg
      121 cgaagatcga caccctctac gagggcacga cggccatcca gggcctggac ttcttcttcc
      181 gcaagatcgt gcgcgacaag ggccaggcgc tgaccttcct gtcctcgcag atgcaggagt
      241 tcgccaagga cctcggcgcc cacgacggcc gcctcgaccg cgaccgcgag ctgctcggcc
      301 agggcctgga ggacgtgcag ggcatcctcg ggttcatggt cggcgagctg atgaagtccg
      361 acccgcgcaa cggcggcgag atcacgaaca tctactcggt cggccagaac acctcgcggc
      421 tgctgctgtc cgcgggcgac ctcgtcgtcg ggtggctgct gctgcgccag gccgcggtgg
      481 ccctggacgc cctggccgcc ggtgccaccg gcaaggacaa ggacttctac gagggcaagg
      541 tcgcggcggc gcagttctac gcccggcagg tgctgccccg cctgagcgcc gagcgggcga
      601 tggccgaggc caccgactcg atgctcatgg acgttcccga gtcggccttc tgagtcgccg
      661 ccccatcggt cggggccttc gggcctcgac ccctgaaccc ggggcgggcg cgcagcccct
      721 ttgcgtgtgc ccgccccgcg ccacgagaac cgaacggccc accccgcgtg cggggtgggc
      781 cgttcgtcgt tgcggctctg ggcgcaggct cagcgggggt cgacgtcggt gcggtcgatg
      841 cggctgccgg ttgcggggtc gacgtggctc tcacgggtgg ccgagtagcc ggccgcggtg
      901 cggcgggtcg cgaggaagga cagcaggatc gccacgacgc ctgcgcccat gaggatgtag
      961 cccagggtgt cgtcgctgac ccccgggatg tcgatgttca gggcgaaggc gaggatggcg
     1021 ccgaggacga acaggacgat gccgagtccg atgtacatga gtgctcctgg gtgggggaga
     1081 aagtgccggg ttcggctgga cccatcgacg ctacccgcgg gtagtgcttg aaatgtgtcc
     1141 taggtgcacg ccagttgctg agtcgggggt ggtcggatgc cgtgcggcag gtcgagcgcg
     1201 acgcggaagt tcgggtgccg cgcgatagtt cggatgccgc gcagcacttc gatcggcacg
     1261 caccagttcg gatcgccgcc gggaattact gcgtgcgctc cgagctcctg cgttctaann
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ggccatggcc gcgatggcca
     1441 gcacgactcc gatgatcaac caccacatga cggcctcctt cggcccggcc gactgcgggt
     1501 cggcgttgct gccacgatcc cccgccgctg caaagctgtc catggtcgtt tcgggccaac
     1561 ccgcgccgcc ggtcagacga aggagcccgg gttgaggatg ccgtgcgggt cgagggccgc
     1621 cttgacgcgc cggttgagtg ccatgacgtc cggaccgagc tgcgcctcga gcgagccggc
     1681 cttgaggcgg cccaccccgt gctcgccggt gatggtgccg ccgagggcga tggccgcggc
     1741 catgatgtcg tcgaaggcga ggcgcgcgcg gcgctcggca tccgcgtcac cggcagggta
     1801 gacgatggcc gggtgcgtgt tgccgtcccc ggcgtgcgcc accatgggga tctcgaggtc
     1861 gtgccgggcc gcgatctcgg cgacggccgc gagcagtgcc gggagctgcg gcaccggcac
     1921 gccgacgtcc tcgggcagca cgttcccacg cgcctcgatc gcggtgaagt gcagccgcct
     1981 ggcccgcacg aacagctcgc cctcggccgg gtcgtccgtc gcgaagacct cgctggcccc
     2041 ggcgccctcg caggcggcga ccgcggccgc cacctcgtcg gcccgggccg cgctgggagc
     2101 gtcggactgg atgagaagga gcgccccggc atcccgattc aggcccatgg gggtgtggtc
     2161 ctcgacggcg ttgatggagc cgtggtccat gagctcgacc atcgaggcgc ggaccgttcg
     2221 gcccagggcg atcaccgcgt cggctgcggc ggcgacgctg gggaaggtcg cgacgacggt
     2281 cgccggcggc tgctgcccgg gcacgagccg caggatggcc ctggtgacga cgccgagcgt
     2341 gccctcggag ccgacgaaga gcttgaggag tgacaacccg gcgacgtcct tgacgcgttt
     2401 gccgccgagg gtgaccggcg tgccgtcggc gaggacgacg tcgaggccga gcacgtagtc
     2461 ggtggtcacg ccgtacttca cgcagcacag cccgcccgcg ttggtggcga tgttgccacc
     2521 gatcgagcac atctcgaacg agctcgggtc gggcgggtac cagaggccgt gctcggcggc
     2581 ggcgcgcttg acctcgacgt tcagggcgcc gggctcgacg acggcgaccc gggcgtcgac
     2641 gtcgatctcg accgcgcgca tccgctcggt cgacagcacg atgccgccgt cgaccgccag
     2701 tgccccgccg gagaggcccg acccggcgcc ccgggggacg acggggatgc cgtgccgcgt
     2761 cgcccagcgc atcgtcgtca cgacgtggcc ggcgttctcg gcgcgcacga cggccgcggg
     2821 gtgaccggcg cgggcgtcgt gcgtccagtc gaagcggtac ttctcggcgg catccggatc
     2881 ggtgacgacg accccgcttg gcagggaggc gaccaactcg gcgacggcgc gctccacggc
     2941 atccctggca tccatggccc caccttcgca gactcggccg agcgaccggt gctgctcgac
     3001 cgacccgccg acctggctca gccgacgcac tcaaccggct caggtggcga accgggctgg
     3061 ctcaggtggc gagccgaccc gaagggggta cgtccacgaa ccgtttcacc cgtcaaggaa
     3121 tcccttgcgc acggcaacga cccccacagg cacccccacg accctccaca gcccgggctc
     3181 tccccacccg aacgcatgaa ccgtcgccgc cccagcggcc ctgagaggct gggtcagagg
     3241 cgacggtcgc cgagcaccgg gaaggcctcg cggtactccg ttatcacagc gaggtcgacg
     3301 tccaccgaga gcacgcactc cacggctcgc tcgccatcgc cacccgaacg gcgctcgtca
     3361 ccgccagcct cggcgagcac cgcgcccgtg gccgacacga cctggctgtg cccacccatc
     3421 tcgtacccgc cgtgcgaccc cgccgtgttg cactgcacga ccacggcctg gttctccacg
     3481 gcccgcgccc tgccgagcag cgtccagtgc gccacgcggg ccgcgggcca cgccgccggg
     3541 atgacgaaca gctcggcgcc cgcggccacc tgtcgccggt agagctcggg aaagcgcagg
     3601 tcgtaacagg tcgacagccc cgcggtcacc gagccgccgt cgcgcagggg gatgccggtc
     3661 acgacgacct cgtcgccggc ctccatgagc ttgggttcgc cggaaccgaa cccgaagcgg
     3721 tggatcttcc ggtagctcgc gatcacctgg ccgtcagcag cgaacagcag tgacgtgttc
     3781 gccaggtcgt tcccgtccac cccggcctcg ggcagccgct cgacgaacga gccggcgtgc
     3841 agcacgaccc ccgccgtgcg cgcggcatcc gccatcgccc gggcccacgg gccgtcgagc
     3901 ggctgggcgg cgcctggcca gcggctgtag tcgaagcccg tcgcacccca cagctcgggc
     3961 agcacgacga ggtcgtgccc ggcctgggct gcaacgagcc cggccacccg ctcgacccgc
     4021 tgctcgaagg actcctcgtc gccgtaggcg agctgaacga ccgcgatccg catcccccca
     4081 tcctgccagc gcagactcag cgctcggtct ccccacgcag ctgcgccagg tgctcgttgt
     4141 acgcccgcaa ctgcgcgtca ccgtcgcgat cggcctggcg gtccaggcgc cgcgactcgg
     4201 cctcgtcccg ccggaaccac tgcacggcca cgatcaccgc gagcaccagg gtcggggcct
     4261 cgccgacccc ccaggcgatc tcaccggcgc gctgctggtc ggccagggga tccggacccc
     4321 acggcaggtt cagctgggtg aagaagtcgg gggccagcaa ggtgtcgctg ccggtcagcg
     4381 ccaccccgaa gaaggcgtgg aaggagatcg tcgcgaagag gatgacgagc agcatgagcg
     4441 gg
//
LOCUS       DAOSOC010000184         4399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_19929_length_4399_cov_6.889042, whole genome shotgun sequence.
ACCESSION   DAOSOC010000184 DAOSOC010000000
VERSION     DAOSOC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19929_length_4399_cov_6.889042"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..704
                     /locus_tag="PL014_11335"
     CDS             84..704
                     /locus_tag="PL014_11335"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT57727.1"
                     /translation="MSRTSVSDPDPAPGPAPDFATKPTLRGQHVTLRPMTGEDAEAMA
                     QILSDPQVRLLTGSVETTAQAHEPWSLDDTLVQWYSSRPDQDDRLDLAIEDEAGSLVG
                     EVVLNDYDPQERTCNLRVLIGPAGRDRGLGTEAVALTTSYGIRDLGLTRITLEVLDTN
                     PRGRRVYEKVGYRPTGHRDQAAVLDGQDIGALDMSVDADTWPGYPA"
     gene            701..3829
                     /locus_tag="PL014_11340"
     CDS             701..3829
                     /locus_tag="PL014_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57728.1"
                     /translation="MRNGLRQTWAAVRHRRSQSLVLVVVSALVTTCAVFAPLFVRTLE
                     QGLLQAGLQQRDVADTTVVVRAARTAQDPALSPDDLRAVMPEEATRWFDDGVGMTTAD
                     TRVTPRDGMQTSPLRLVARQGSCEHVEIVSGSCPTAPGDVLVSAADATAWGWAEGSTF
                     EVADSSAFPATPSDAVAPVALEVTGVYRVVPDPTYWLRTVVDGKSGFLIAEGTNTVAA
                     IDDFLTAQATFDTGWSQASTSVEFPLDRDTVTLATVPQISDSLQGLERASGNVTVTSP
                     VPDIVDAVVAGRSIVRALVPLLLAQLALLAVTVLALMAHAAVEQRRPEIALARLRGRS
                     RDGGGRVVMGELALTVALGAPLGVALALAVGDLLRRTVMPPGVPFELRWPVAVAAVVA
                     VLGSLLAVYVVSRPVLREPIGALLRRVPPQASRGLGMLDVVVIVVAALGVAAVLTGDV
                     DGPTALLVPMLLALAVGLLGAAALRRLASRVADRTLARGRLPAGLAALSLARRPALRN
                     VLVVVTAAVALATFAANAVVVGAHNRAARAELENGAPAVITTDATSPAALAAAVDGLE
                     PRYRSLATPVVVIRPRDPSAVPTLLARPAELARIGFALPTASLPALTPEVAPSIQLTD
                     GVITGTLTWALEDFRTGDEPVGKAPRSPTGIPGGDLSVNPTPVQIGITVSMPDGELLD
                     RDLAQVPQSSAGTSAVRAPVLCPQGCRLAGLWVRGTDPWAERVAGRVTLSGLSLGGEP
                     LELGGAQRWLPAQGDPGEGTQALSGSGTDLVIDVETTGRRVFSPVADVPSPAPVVLAG
                     TPPADATDDGFTLIGLGGRPVASQAVATVDALPGVTSRGALADLDAQVRVGGAAPPGS
                     LLQVWLATQDPDELRAVERSLAAAGIPVGPATTVEQAQARYDASATGWGLLLAVFTGL
                     MSLLVAALVVGLVAVTSWRGVARDLAGLIVAGTPRAVLRAAARGEHLVTVVAGVVLGT
                     ACGIAGSLLAMPLLPLFDRPAPVPVPDLAPVWWVIAATALVALVVIGGVAVVAARAVM
                     ARALPERLRESL"
     gene            3826..>4399
                     /locus_tag="PL014_11345"
     CDS             3826..>4399
                     /locus_tag="PL014_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT57729.1"
                     /translation="MSGLRVVTRGLVHIYHADGHDVAALSGIDLVVGAGERLALLGPS
                     GAGKSTLLTLFGGLLRPSAGRLLVGDRELSQLTEAELDEVRGTEVAMVLQGAARNLLP
                     YLSARGNVTFAQGAARQRGRKVPTPDEVLDLVGFPAASADGPLEHLTPGALQLLAVAV
                     AVSCRPGLLLGDEPTSQLDHGARDVVLEALA"
BASE COUNT          482 a         1697 c         1578 g          642 t
ORIGIN      
        1 ggccgcagcg acccgtacgc cgcggccgat gcgctggtgc gctccctcac gtgagatcgg
       61 ccggcgtccg gcaggatgtc ggggtgtccc gcacgtccgt cagcgatcct gaccccgctc
      121 ccggccccgc gcccgacttc gccacgaagc cgacgttgcg cggccagcac gtgacgttgc
      181 ggccgatgac gggcgaggac gccgaggcga tggcgcagat cctctccgac ccgcaggtgc
      241 gtctgctgac cggcagcgtc gagaccaccg cgcaggcgca tgagccctgg tccctcgacg
      301 acacgctcgt gcagtggtac tcctcgcgcc ccgaccagga cgaccgcctc gacctggcca
      361 tcgaggacga ggcagggtcc ctcgtcgggg aggtggtgct caacgactac gacccgcagg
      421 aacgtacgtg caacctgcgc gtcctcatcg ggcccgccgg gcgtgaccgg gggctcggaa
      481 ccgaagccgt cgcgctcacg acgagctacg gcatccgaga cctcggcctc acccgcatca
      541 ccctcgaggt gctcgacacc aacccgcgcg gccgccgcgt ctacgagaag gtcggatacc
      601 ggcccaccgg ccaccgggac caggctgccg tgctcgacgg gcaggacatc ggcgccctcg
      661 acatgtcggt cgacgccgac acctggcccg ggtacccggc gtgaggaacg ggctgcggca
      721 gacgtgggcc gcggtgcgcc accggcgcag ccagtcgctc gtgctcgtcg tcgtctcggc
      781 cctggtgacg acctgcgccg tcttcgcccc gctgttcgtg cgcaccctcg agcaggggtt
      841 gctccaggcg gggctccagc agcgtgacgt cgccgacacc accgtcgtcg tgcgggcggc
      901 gcgcaccgcg caggaccccg ccctcagccc cgacgacctg cgcgcggtca tgcccgagga
      961 ggccacccgg tggttcgacg acggcgtcgg catgacgacg gccgacacca gggtcacgcc
     1021 ccgtgacggg atgcagacct cgcccctgcg cctcgtggcc cgccagggca gctgcgagca
     1081 cgtcgagatc gtgtcgggct cgtgcccgac ggcacccggc gacgtcctcg tctccgcggc
     1141 cgacgcgacc gcgtgggggt gggccgaggg ctcgaccttc gaggtcgccg actcgtccgc
     1201 gttccccgca accccatcgg atgccgtggc accggtcgcg ctggaggtca ccggggtcta
     1261 ccgcgtcgtc ccggacccca cctactggct gcggacggtg gtcgacggca agtccggctt
     1321 cctcatcgcc gagggaacca acaccgtcgc ggcgatcgac gacttcctca ccgctcaggc
     1381 caccttcgac accggctggt cgcaggcctc gacgtcggtg gagttcccgc tcgaccggga
     1441 caccgtgacg ctggcgacgg tgccgcagat cagcgactcc ctgcaggggc tggagcgggc
     1501 gagcgggaac gtcacggtga cctccccggt gcccgacatc gtcgacgccg tcgtcgccgg
     1561 ccgctcgatc gtgcgcgccc tggtgccgct gctgctcgcg cagctcgcac tgctcgcggt
     1621 cacggtgctc gcgctcatgg cccatgccgc cgtcgaacag cgccgacccg agatcgccct
     1681 tgcccggttg cgcgggcgca gccgtgacgg gggcgggcgc gtcgtcatgg gtgagctggc
     1741 gctgacggtg gcgctgggtg ccccgctcgg ggtcgcgttg gcccttgcgg tcggtgacct
     1801 gctgcgccgc accgtcatgc cacccggggt tcccttcgag ctgcgctggc cggtcgcggt
     1861 ggctgcggtc gtcgccgtgc tggggtcgct gctcgcggtg tacgtcgtgt cccgcccggt
     1921 ccttcgcgag cccatcgggg cgctgttgcg ccgcgtccca ccgcaggcca gccggggcct
     1981 ggggatgctc gacgtcgtcg tcatcgtcgt cgccgctctc ggggtggcgg cggtgctgac
     2041 cggcgacgtc gacggaccca cggcgctgct cgtccccatg ctgctcgcgc tggccgtcgg
     2101 cctgctcggg gccgccgccc tgcgccgcct cgcctcccgg gttgcggatc ggaccctcgc
     2161 ccggggtcgc ctgcccgccg gcctcgcggc cctgtccctg gcgcgccggc cggccctgcg
     2221 caacgtcctc gtcgtcgtca cggcggccgt ggctctcgcg accttcgcgg cgaacgccgt
     2281 ggtcgtcggg gcgcacaacc gtgccgcccg ggccgagctc gagaacggcg ccccggcggt
     2341 catcacgacc gacgccacct cacccgccgc actggcggcc gcggtcgacg ggctcgagcc
     2401 gcgctaccgg tcgctcgcca cccccgtggt cgtcatccgt ccccgggacc cctcggccgt
     2461 accgaccctg ctcgcccgcc ccgccgagct cgcacgcatc ggtttcgccc tgcccaccgc
     2521 gagcctgccc gccctgacgc ccgaggtggc gccgagcatc cagctcaccg acggcgtcat
     2581 caccggcacg ctcacctggg ccctcgagga cttccgaacc ggcgacgaac cggtcggcaa
     2641 ggcaccgagg tcacccaccg gcatccccgg tggtgacctg tcggtcaacc cgaccccggt
     2701 ccagatcggg atcaccgtgt cgatgcccga cggtgagctc ctcgaccgtg acctcgcgca
     2761 ggtgccgcag tcctcggcag ggacgtcggc ggtccgtgcc cccgtgctgt gcccgcaggg
     2821 atgccgtctg gccgggttgt gggtgcgcgg caccgatccg tgggccgagc gggtcgccgg
     2881 gcgggtcacg ctctccggtc tcagcctcgg tggcgagccc ctcgagctcg gcggcgcgca
     2941 gcggtggctg ccggcccagg gtgacccggg ggagggcacc caggcgttgt ccggctcggg
     3001 caccgacctc gtcatcgacg tcgagaccac cgggcgccgg gtgttctcgc ccgtcgcgga
     3061 cgtgccgagc ccggcccccg tggtgctcgc cggcacccct cctgccgatg ccaccgacga
     3121 cggcttcacc ctcatcggcc tcggggggcg gcccgtcgcc tcgcaggccg tggcgacggt
     3181 cgatgcgctg ccgggggtga cctcccgggg tgcgctcgcc gacctcgacg cccaggtgcg
     3241 ggtgggtggt gcggccccgc cggggtcgct gctccaggtg tggctggcca cccaggaccc
     3301 tgacgagctt cgtgccgtcg agcgctcgct ggctgccgcc gggatccccg tgggcccggc
     3361 cacgacggtg gagcaggcgc aggcccgcta cgacgcctcg gccaccgggt ggggcctgct
     3421 gctcgcggtg ttcaccgggc tgatgtccct gctcgtggcc gccctcgtcg tcgggctcgt
     3481 ggccgtgacg tcgtggaggg gggtggcccg tgacctcgcc ggactcatcg tggccggcac
     3541 cccgcgggcg gtcctgcgcg ccgcagcgcg tggcgagcac ctcgtgaccg tggtcgccgg
     3601 tgtggtgctc ggcaccgcat gcggcatcgc cgggtcgctg ctggcgatgc cgctcctgcc
     3661 gttgttcgac cggccagccc cggtaccggt gccggacctc gccccggtct ggtgggtgat
     3721 cgccgcgact gcgctggtgg ctctcgtggt catcggcggg gtggccgtgg tcgccgcacg
     3781 ggccgtcatg gcccgtgcgc tgcccgaacg actgcgggag tcgctgtgag cggcctgcgc
     3841 gtggtgacgc gcggcctcgt gcacatctac cacgctgacg ggcacgacgt cgctgcgctg
     3901 tcgggcatcg acctcgtcgt gggggcgggg gagcggttgg cgctgctcgg cccgtccggt
     3961 gctgggaagt cgacgttgct caccctgttc ggcggcctgc tgcgcccgag cgccgggcgc
     4021 ctgctcgtcg gcgaccgcga gctcagccag ctcaccgaag ccgaactcga cgaggtccgc
     4081 ggtaccgagg tcgcgatggt cctccagggt gcggcacgca acctgctgcc ctacctctcg
     4141 gcgcggggca acgtcacgtt cgcccaaggg gcagcgcgcc agcggggccg taaggttccc
     4201 acgcccgacg aggtcctcga cctcgtcggc ttccccgcgg catccgccga cggcccgctc
     4261 gaacacctga cccccggtgc ccttcagctg ctcgccgtgg ccgtcgccgt ctcgtgccga
     4321 ccggggttgc tgctcggcga tgaaccgacg agccagctcg accacggggc gcgcgacgtc
     4381 gtgctcgagg cactggcgc
//
LOCUS       DAOSOC010000185         4384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_20016_length_4384_cov_9.049896, whole genome shotgun sequence.
ACCESSION   DAOSOC010000185 DAOSOC010000000
VERSION     DAOSOC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20016_length_4384_cov_9.049896"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..967)
                     /gene="rarD"
                     /locus_tag="PL014_11350"
     CDS             complement(2..967)
                     /gene="rarD"
                     /locus_tag="PL014_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOT57730.1"
                     /translation="MTPPPSGAAPTGGGEAELRRGTAYAFLAYGIWGMFPLYFAALEP
                     SGAWEILAHRILWTLAVVALVLLVRRDLAWIRPFLSRPRLVAGITLAALLIAGNWVVY
                     VLAVLTGRTYEAALGYFLNPIVTVGLGVLVLGERLRRLQWAAVAVGAVAAVYLTVAGG
                     VLPWISLSLAFSFGLYGLVKKRVGASLEALHSLAAETAVLAPVAVGVIVVLSLQGATT
                     FAGHGVAHTTLLVLAGVVTAVPLLLFAAAARRIPLVTVGLIQFITPMLQLLVGVTLLG
                     EHVSGRLWFGFGIVWVALALLTFDSLRAARAARAGRREPPVEPCP"
     gene            complement(964..2040)
                     /locus_tag="PL014_11355"
     CDS             complement(964..2040)
                     /locus_tag="PL014_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOT57731.1"
                     /translation="MPVSGLRSVPHLPDDADKQTKRDFTSDQEVRWCPGCGDYVILAA
                     VQSFLPELGLKRENIVFVSGIGCSSRFPYYLDTYGMHSIHGRAPAIATGLATSRSDLS
                     VWVVTGDGDALSIGGNHLIHALRRNVNMTILLFNNQIYGLTKGQYSPTSKVGQVTKSS
                     PVGSVDSPFNPVSLALGAEATFVARAMESDRAELTTILKAAAEHRGTALVEIYQNCPI
                     FNDGAFEVLKDRTQQEARIVRLRAGEAVTVGAEGERKVLVRQGSGVMKFIAEAEAASG
                     EKGAVVVHDPALDDPSQAFQLSRLDSPEMTHVPMGIFRQVDRPTYDDLVRAQVDAAVS
                     TAGGPATDADLTDLLLGRDTWTVG"
     gene            complement(2061..3926)
                     /locus_tag="PL014_11360"
     CDS             complement(2061..3926)
                     /locus_tag="PL014_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOT57732.1"
                     /translation="MSSKTVQTLDRVVIRFAGDSGDGMQLTGDRFTSDTAALGNDLST
                     LPNFPAEIRAPQGTLPGVSSFQLHFADHDVLTPGDAPDVLVAMNPAALKANLGDLPRG
                     ATIIVNTDEFTKRNLAKVGYSDNPVDDGTLDSFHVHAVALTSITVEALAEFPTLTRKE
                     KERAKNMFALGLLSWMYTRPTTGTEAFLKSKFGARPEILAANLAALTAGWNYGETTED
                     FASSFIVPAATMPAGTYRNITGNTALSLGLVAAAHRAGIPLVLGSYPITPASDILHAL
                     AALKRHGVTTMQAEDEIAGIGIALGASFGGAIGVTTTSGPGVALKSEAIGLAVSLELP
                     LIIVDVQRGGPSTGLPTKTEQADLLQAMFGRNGESPVPIVAPKTPADCFTAAMDAVRI
                     ATTYRTPVFLLSDGYLANGSEPWSVPSVDSLPDLTVEFASEPNGVDPKGEPTFHPFLR
                     DEQTLARPWAVPGTAGLEHRIGGIEKADVTGNISYDPDNHDLMTRLRQAKVDGITVPD
                     LVVDDPSGQARVLVLGWGSTFGPIAAATRVVRNAGHPVAQAHLTHLNPFPANLGEVLK
                     SYDRVIVPEMNLGQLSMLLRAKYLVDIRSHTSVRGLPFSTVELADVISTTLEELS"
     gene            4198..>4384
                     /gene="ndhC"
                     /locus_tag="PL014_11365"
     CDS             4198..>4384
                     /gene="ndhC"
                     /locus_tag="PL014_11365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOT57733.1"
                     /translation="MEGYAAAAAVVLAGVLLVTAAMVARRLLAPRAVTEAGLRTYESG
                     VDPVGTGWAQSQIRYIAF"
BASE COUNT          665 a         1393 c         1695 g          631 t
ORIGIN      
        1 gtcaggggca gggttcgacc ggcggctcgc gacgccccgc gcgcgctgcc cgggccgcac
       61 gcagcgagtc gaaggtcagc agcgccagtg ccacccagac gatcccgaag ccgaaccaca
      121 ggcggccgct cacgtgctca ccgagcaggg tcaccccgac gagcagctgg agcatcgggg
      181 tgatgaactg gatgaggccg acggtgacca gggggatccg ccgggcagcc gcggcgaaca
      241 gcagcagcgg cacggccgtg accaccccgg cgagcacgag cagcgtcgtg tgcgcgacgc
      301 cgtgcccggc gaaggtcgtc gcaccctgca gcgacagcac gacgatgacg ccgacggcca
      361 ccggtgcgag caccgcggtc tccgcggcca acgagtgcag cgcctcgagg ctcgcgccga
      421 cccgcttctt caccagcccg tagagaccga acgagaacgc caggctgagg ctgatccacg
      481 gcagcacccc gccggccacc gtgaggtaga ccgccgccac cgcgcccacg gccaccgccg
      541 cccactgcag gcgcctgagc cgttcgccga gcacgagcac gccgaggccg acggtgacga
      601 tggggttgag gaagtagccg agggcagcct cgtacgtgcg accggtgagc acggcgagca
      661 cgtagacgac ccagttcccg gcgatgagca gggccgccag cgtgatgccg gcgacgagac
      721 gaggtcgtga caggaacggc cggatccacg cgaggtcgcg ccgcacgagc agcacgaggg
      781 cgacgacggc caacgtccac aggatgcggt gggccaggat ctcccaggca ccggagggtt
      841 cgagggcagc gaagtacagg gggaacatcc cccagatgcc gtaggcgagg aaggcgtatg
      901 ccgtgccacg ccgtagctcg gcctccccac ctcccgtcgg ggccgcgccc gacggaggcg
      961 gggtcacccc acggtccagg tgtcgcgtcc gagtagcagg tcggtgaggt cggcgtccgt
     1021 ggcggggccc ccagcggtgc tgaccgcggc gtcgacctgg gcacgcacga ggtcgtcgta
     1081 ggtgggccgg tcgacctggc ggaagatgcc catgggcacg tgcgtcatct cgggggagtc
     1141 cagccgggag agctggaagg cctgcgacgg gtcgtcgagc gccgggtcgt ggacgacgac
     1201 ggcacccttc tcgccggacg cggcctctgc ctcggcgatg aacttcatga cgcccgagcc
     1261 ctgccggacg agcaccttgc gctcaccctc ggcgccgacg gtgacggcct cgccggcgcg
     1321 cagccgcacg atgcgcgcct cctgctgggt gcggtccttg agcacctcga aggcgccgtc
     1381 gttgaagatc gggcagttct ggtagatctc gaccagggcg gtgccccggt gctcggcggc
     1441 cgccttgagg atggtggtga gctcggcgcg gtcggactcc atcgcccgcg cgacgaacgt
     1501 cgcctcggcg ccgagcgcca gcgacacggg gttgaacggg ctgtcgaccg acccgaccgg
     1561 gctggacttg gtgacctgac cgaccttcga cgtgggggag tactgcccct tggtcaggcc
     1621 gtagatctgg ttgttgaaca gcaggatcgt catgttgacg ttgcggcgca gtgcgtggat
     1681 gaggtggttg ccgccgatcg acagggcgtc gccgtcgccg gtgacgaccc acaccgacag
     1741 gtccgaacgg ctcgtggcca ggccggtggc gatcgccggg gcacgcccgt ggatcgagtg
     1801 catgccgtag gtgtcgaggt agtacgggaa gcggctcgag cagccgatgc cggagacgaa
     1861 cacgatgttc tcgcgcttga ggcccagttc gggcaggaag gactgcaccg cggcgaggat
     1921 gacgtagtca ccgcacccgg ggcaccaacg cacctcctgg tcggaggtga agtcgcgctt
     1981 ggtctgcttg tcagcgtcgt ccgggaggtg ggggaccgaa cgaaggccgc tgaccggcat
     2041 ccccaaatcg gtggtggtgc tcacgagagc tcctcgaggg tggtggagat gacgtcggcg
     2101 agttcgacgg tggagaacgg aagcccacgc accgaggtgt gcgagcggat gtcgacgagg
     2161 tacttcgcgc gcagcagcat cgagagctgg ccgaggttca tctcggggac gatgacccgg
     2221 tcgtacgact tcagcacctc gccgaggttt gcagggaagg ggttgaggtg cgtcaggtgg
     2281 gcctgcgcaa cggggtgccc ggcgttgcgg acgacccggg tcgcggccgc gatcgggccg
     2341 aaggtcgatc cccagccgag cacgagcacg cgagcctggc cgctcgggtc gtcgacgacg
     2401 agatcgggca cggtgatgcc gtcgaccttg gcctggcgca gccgggtcat caggtcgtgg
     2461 ttgtcggggt cgtaggagat gttgccggtg acgtcggcct tctcgatgcc gccgatccgg
     2521 tgctccagac ccgcagtgcc gggcacggcc cacggccggg ccagcgtctg ctcgtcgcgc
     2581 aggaacgggt ggaacgtggg ctcgcccttg gggtcgaccc cgttgggttc gctcgcgaac
     2641 tcgacggtga ggtcgggcag ggagtcgacc gacgggaccg accacggctc ggagccgttg
     2701 gcgaggtagc cgtcggacag caggaacacg ggggtgcggt acgtcgtcgc gatgcgaacg
     2761 gcatccatcg cggcggtgaa gcagtcggcc ggggtcttcg gcgcgacgat cggcaccggg
     2821 gactcgccgt tgcggccgaa catcgcctgc agcaggtcgg cctgctcggt cttggtcggc
     2881 agtccggtgg acgggccacc acgctggacg tcgacgatga tgagcggcag ctcgagggag
     2941 acggccaggc cgatcgcctc ggacttcagc gccacccccg gccccgacgt cgtcgtgaca
     3001 ccgatggcgc caccgaagct ggccccgagg gcgatgccga tgccggcgat ctcgtcctcg
     3061 gcctgcatcg tcgtgacgcc gtggcgcttc agcgcagcga gcgcgtggag gatgtcggat
     3121 gccggggtga tggggtagga gccgagcacc agggggatgc cggcgcggtg cgcagcggcg
     3181 accaggccca gggagagggc ggtgttgccg gtgatgttgc ggtaggtgcc ggccggcatc
     3241 gtcgcggcgg ggacgatgaa ggaggaggcg aagtcctcgg tggtctcgcc gtagttccag
     3301 ccggcggtga gagcggcgag gttcgccgcg aggatctcgg ggcgggcgcc gaacttgctc
     3361 ttgaggaagg cctcggtgcc ggtcgtcggc cgggtgtaca tccacgagag caggccgagc
     3421 gcgaacatgt tcttcgcgcg ctccttctcc ttgcgggtga gcgtcgggaa ctcggccagg
     3481 gcctccacgg tgatgctcgt gagggccacc gcgtgcacgt ggaaggagtc gagggtgccg
     3541 tcgtcgacgg ggttgtcgct gtagccgacc ttggcgaggt tgcgcttggt gaactcgtcg
     3601 gtgttgacga tgatcgtcgc cccgcggggc aggtcgccga ggttggcctt gagggcggcc
     3661 gggttcatcg cgacgagtac gtcgggggcg tcaccggggg tgagcacgtc gtggtcggcg
     3721 aagtgcagct ggaagctgga gacgccgggc agcgtgccct ggggggcgcg gatctcggcc
     3781 gggaagttgg gcagcgtcga caggtcgttg cccagggcag cggtgtcgct ggtgaaccgg
     3841 tcaccggtca gctgcatgcc gtcgccggag tccccggcga agcggatgac gacgcggtcg
     3901 agcgtctgga cggtcttgct gctcacgggg caccctgtgg tcaagtcggc gacggaaact
     3961 cacgtgctgt gccgccggtt ctgccgcggg cgcagcgttc tccatggtac gtcgcgatga
     4021 gggtagcctc accgctgtcc acgaggtgtc ggcccgcgta ccccgagtga cctattggtc
     4081 ggtggaggtg cggatgccgg gtggtcggtg gtgtaccccg agtgacctat tggtcggtgc
     4141 aggtacgggc gggcgaggct cgctcaccgc ggccgaggcg cactaggctc ggtgcccatg
     4201 gagggctacg cggcagcagc ggcagtggtg ctcgccggcg tgctgctcgt caccgccgcg
     4261 atggtcgccc gccgactgct tgcgccccgg gccgtcaccg aggccgggct gcgcacctac
     4321 gagagcggcg tcgaccccgt cggcacgggc tgggcgcaga gccagatccg ctacatcgcc
     4381 ttcg
//
LOCUS       DAOSOC010000186         4293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_20568_length_4293_cov_7.969797, whole genome shotgun sequence.
ACCESSION   DAOSOC010000186 DAOSOC010000000
VERSION     DAOSOC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20568_length_4293_cov_7.969797"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PL014_11370"
     CDS             <1..324
                     /locus_tag="PL014_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT57734.1"
                     /translation="PGALMSAASWVEAGIVADARSARHGERLDRVIEELGIEIVPVTT
                     RQAEVARLAYQRFGRGSGSPARLTYGDCFSYALSTTEGRPLLYVGNDFSQTDVAPALR
                     ADWSP"
     gene            321..>1737
                     /locus_tag="PL014_11375"
     CDS             321..>1737
                     /locus_tag="PL014_11375"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOT57735.1"
                     /translation="MTKAPGTSPESVRGLGPKTAAALVFGSSAAVLVVELAALRLLAP
                     YLGLTLETSTIVIGIALAAIATGSWAGGRAADIMPPRRALGPLLAVSGVAVAATPFLV
                     RAAGETGGSGAALLMAIVAILVPGALLSAVSPMVTKLVLTDLAETGSVVGRLSGIGTV
                     GAIAGTVLTGFVFLAALPVSSVLVATGVLLVLAAAAVEVRVRGIRAAVLPLVLLVPGA
                     LGAAYGPGRCEAETVYHCAQVQTDPARESGRVLVLDSLRHSYVDLDDPTHLEFQYVQA
                     LAGVADTAFPAGEPLEAYHLGGGGLTFPRYLERVRPGTVSVVSEIDRGVLAIDERDLG
                     LVTGPAMDVRIEDARLGLRSLDTDSRDLVVGDAFGGISVPWHLTTREALTDVQRVVRP
                     GGIYAANLIDFAPLSFARAELATMRTVFEHVAVAADAATLGREVGSGGNVVAIASDEP
                     LDLEALRSGFAAQGLEWEVVEG"
     assembly_gap    1738..1837
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1838..2625)
                     /locus_tag="PL014_11380"
     CDS             complement(<1838..2625)
                     /locus_tag="PL014_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOT57736.1"
                     /translation="MTGHHSAPRVPVRRKIAVATWRPSRDGRIYARMEVDVTPALAYI
                     ERVREETGERITITHVVGAALGRALRAVPEVRARVVFGRLRPLERCDIGFAVDIDRGS
                     DLAPIKVHDVDRLSPVEIAQALDAGAGKLRRGDHTSYKRTSSLVKIAPAWALRPVMAA
                     AGVVAGGFGKGFVGQPGFPLGTAFVSNVGTLGLDEAFLAPLPLARTPIYLAVGTVQQR
                     AVVVDGEVVARPVLVLVATADHRIVDGAHAGQVTTIVRDLLTHP"
     gene            complement(2622..3404)
                     /locus_tag="PL014_11385"
     CDS             complement(2622..3404)
                     /locus_tag="PL014_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT57737.1"
                     /translation="MRVAGTHVLLTGATGDIGRRLATHLSDAGARLTLVARSTQPLEA
                     LAADLGAHAVPADLTDRDTLRSLIRDVEQAHGPVDVLVNNAGTETVGHLTHLSADDLE
                     HLLALNLLAPVELCRQVLPGMTARGRGHLVNISSLAGVATFPGLATYGATKAGLTHFT
                     SGLRADLRGGPIGTLAVEIGPVSSQMMSRIASHPPTAAAFDRVLGLRALTMLDPDAVA
                     RAVVAAVEDDQATLRMPRRAAPLATLAHAPRSVVRLALTGIR"
     gene            3616..3822
                     /locus_tag="PL014_11390"
     CDS             3616..3822
                     /locus_tag="PL014_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOT57738.1"
                     /translation="MIVCHCHVVNDAAIAAAVDAGARTLGQVCRSTGAGSDCGSCVFS
                     VRRLVCEHVSRQVPESVEAQHAAS"
     gene            3809..4291
                     /gene="bfr"
                     /locus_tag="PL014_11395"
     CDS             3809..4291
                     /gene="bfr"
                     /locus_tag="PL014_11395"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOT57739.1"
                     /translation="MRPVDPKIVDHLNDALTFELSVVNTYFLHARMLDSWGFTKLGKV
                     FYDLSIDEMRDADSLITRILFFEGHPNIQRLNALHVGESAEEMLRLALESEYAAVAQF
                     NAAAQECHDLGDHGTASLFETMVLDEEKHADWFEGQIEAIARIGLPQYLAQQVETTTP
                     "
BASE COUNT          545 a         1447 c         1575 g          626 t
ORIGIN      
        1 ccgggggcgc tgatgtcggc ggccagctgg gtcgaggcgg gaatcgtcgc ggacgctcgc
       61 tccgcgcgcc acggggagcg cctcgaccgg gtgatcgagg agctggggat cgagatcgtt
      121 ccggtcacca cccgccaggc cgaggtggcg cgactggcct accagcgctt cggtcgcggg
      181 tcgggttcac ctgctcgcct cacctacggc gactgcttct cctacgccct gtccaccacg
      241 gagggtcgac ccctgctcta cgtcggcaac gacttcagcc agaccgacgt cgctccggcg
      301 ctgcgtgcag actggtcgcc atgacgaaag ccccaggcac atccccggaa tctgtgcgtg
      361 ggctggggcc gaagacggcg gctgcattgg tgttcggctc gagtgcggcc gtgctcgtcg
      421 tcgagctcgc ggcgctgcgg ctgctcgccc cctacctcgg cctcaccctc gagacgagca
      481 cgatcgtcat cggcatcgcg ctggccgcga tcgccacggg gtcgtgggcc gggggccggg
      541 ccgccgacat catgccgccg cgtcgtgcgc tcggcccgct gctcgccgtc tcgggggtgg
      601 ccgtggccgc gaccccgttc ctcgttcggg ccgccggtga gaccggaggg tcgggagcgg
      661 cgctgctcat ggcgatcgtg gcgatcctcg tgccgggtgc cctgctgtcg gccgtgtccc
      721 cgatggtgac gaagctcgtg ctcacggacc ttgccgagac cggcagcgtc gtcggccggc
      781 tgtcgggcat cggcacggtc ggtgccatcg ccgggacggt gctcaccggg ttcgtgttcc
      841 tcgcggcgct gccggtcagc tcggtgctcg tcgccaccgg ggtgctgctc gtgctggccg
      901 cggcggccgt ggaggtgcgc gtgcgcggca tccgggctgc ggtgctgccg ctcgtgttgt
      961 tggtgccggg ggcgctcggc gcggcctacg ggcccggccg gtgcgaggcc gagacggtct
     1021 accactgcgc gcaggtgcag acggatccgg cgcgcgagag cggtcgggtg ctcgtgctcg
     1081 acagcctccg ccactcctac gtcgacctcg atgacccgac ccacctcgag ttccagtacg
     1141 tgcaggcgct cgccggtgtc gcggacacgg cgttcccggc cggtgaaccg ctggaggcgt
     1201 atcacctcgg cggcggcggg ttgacctttc ctcgctacct cgagcgggtg cggcccggca
     1261 cggtcagcgt ggtctccgag atcgaccgcg gggtgctggc gatcgacgag cgcgacctcg
     1321 gactcgtcac cggcccggcg atggacgtgc gcatcgagga cgcccggctc gggttgcgca
     1381 gcctcgacac cgacagccgc gacctcgtgg tcggcgacgc gttcggtggg atcagcgtcc
     1441 cctggcacct gacgacccgc gaggccctca ccgacgtcca gcgcgtggtg cggcccggcg
     1501 gcatctacgc cgccaacctc atcgacttcg cgccgttgtc cttcgcccgg gccgagctcg
     1561 ccacgatgcg aacggtcttc gagcacgtcg cggtggccgc ggatgccgcg acgctgggtc
     1621 gcgaggtcgg gtccggtggc aacgtggtcg cgatcgcctc cgacgagccg ctcgacctgg
     1681 aggccctgag gtcaggtttt gccgcccaag ggctggagtg ggaggtcgtc gagggcgnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc gggtgggtca gcaggtcgcg
     1861 cacgatcgtc gtgacctgac cggcgtgggc cccgtcgacg atccggtggt cggccgtcgc
     1921 gacgagcacg agcaccggcc gggcgacgac ctccccgtcg acgacgaccg cccgctgctg
     1981 caccgtgccc accgcgaggt agatcggcgt gcgggccagc ggcagcggtg cgaggaaggc
     2041 ctcgtcgagc ccgagggtgc cgacgttgga gacgaacgcc gtgccgagcg ggaagccggg
     2101 ctggccgacg aatcccttcc cgaagccacc ggccacgaca ccggccgccg ccatcaccgg
     2161 gcgcagcgcc cacgcggggg cgatcttcac caggctcgac gtgcgcttgt acgaggtgtg
     2221 gtcaccgcgg cgcagcttgc cggccccggc atccagcgcc tgggcgatct ccacggggct
     2281 gaggcgatcg acgtcgtgca ccttgatcgg cgcgaggtcg gacccgcgat cgatgtcgac
     2341 ggcgaacccg atgtcacacc tctccagtgg acgcagccgc ccgaagacca cccgcgcccg
     2401 cacctcgggc accgcgcgca gggcccgccc gagagccgcc ccgacgacgt gcgtgatcgt
     2461 gatccgttcg ccggtctcct cccgcacccg ctcgatgtag gccagggcgg gcgtgacgtc
     2521 gacctccatg cgcgcgtaga tgcgtccgtc gcgggatggg cgccaggtcg ccacggcgat
     2581 cttgcggcgc accggcaccc ggggtgcgct gtggtggccg gtcatcggat gccggtcagg
     2641 gccaaccgca cgacgctgcg gggcgcgtgg gcgagggttg cgagcggggc cgcgcggcgc
     2701 ggcatccgaa gcgtggcctg gtcgtcctcg acggcggcga cgacggcgcg tgccacggca
     2761 tccgggtcca acatggtcag ggcgcgcagg ccgagcaccc ggtcgaacgc cgctgccgtg
     2821 ggtgggtggc tcgcgatgcg gctcatcatc tgtgaggaga ccggcccgat ctcgaccgcg
     2881 agggtgccga tcgggccgcc gcgcaggtcg gcgcgcaggc cggacgtgaa gtgcgtcagc
     2941 ccggccttgg tcgcgccgta ggtggccagg cccgggaagg tggcgacccc ggccagcgag
     3001 gagatgttga cgaggtggcc gcggccacgg gcggtcatgc cgggtagcac ctggcggcag
     3061 agctcgacgg gggcgagcag gttgagggcg agcaggtgct cgaggtcgtc ggcgctgagg
     3121 tgggtgaggt ggccgacggt ctcggtgccg gcgttgttga cgagaacgtc gaccgggccg
     3181 tgggcctgct ccacgtcgcg gatcagggag cgcagggtgt cgcggtcggt gaggtcggcg
     3241 ggcacggcgt gggcgcccag gtcggcggcg agggcctcga ggggctgggt gctgcgggcg
     3301 acgagcgtga ggcgcgcccc ggcatccgag aggtgggtgg cgaggcgccg cccgatgtca
     3361 ccggtggctc cggtgagcag gacgtgggtt ccggcaactc gcatccgtcc agtgtcgtgc
     3421 acggcgggtc ggtggccacc atcgtgagcg gcctgggggt ggggatgtcg aaccttggtc
     3481 gggctgcggc cccgggcgtt ccggtgtgcg gaatggcgga tgcaccgagt tgagcccgtc
     3541 gggaacaatg tggttaggta agcctcacct tcatccgctg gtgcctcgtg cgcccggccc
     3601 gcgacaggag acgccgtgat cgtctgccac tgccacgtcg tcaacgacgc ggccatcgcg
     3661 gcggccgtcg acgccggcgc gcgcaccctc gggcaggtct gccgctcgac cggggcgggc
     3721 agcgactgcg gctcgtgcgt gttctcggtg cgacggctcg tgtgcgagca tgtctcgcgt
     3781 caggttcccg agagtgtgga ggcgcagcat gcggccagtt gaccccaaga tcgtcgacca
     3841 cctcaacgac gcactgacct tcgagctgag cgtcgtcaac acctacttcc tgcacgcgcg
     3901 catgctcgac agctggggct tcaccaagct cggcaaggtc ttctacgacc tgtcgatcga
     3961 cgagatgcgt gacgcagact ccctcatcac ccgcatcctg ttcttcgagg gccacccgaa
     4021 catccagcgg ctgaacgccc tgcacgtcgg cgagagtgcc gaggagatgc tgcgcctcgc
     4081 cctcgagagc gagtacgccg ccgtcgcgca gttcaacgcc gccgcgcagg agtgccacga
     4141 cctcggcgac cacggcacgg catccctgtt cgagacgatg gtgctcgacg aggagaagca
     4201 cgccgactgg ttcgagggtc agatcgaggc catcgcacgc atcgggctgc cgcagtacct
     4261 cgcccagcag gtcgagacca ccactccctg acc
//
LOCUS       DAOSOC010000187         4248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_20869_length_4248_cov_6.247794, whole genome shotgun sequence.
ACCESSION   DAOSOC010000187 DAOSOC010000000
VERSION     DAOSOC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20869_length_4248_cov_6.247794"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PL014_11400"
     CDS             <1..308
                     /locus_tag="PL014_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57740.1"
                     /translation="GHDDGEEHRDGRGIAVVWLNARDVRRGVQQGSMPWDRTRYVAMY
                     RAVAVAMLLALAATVALSLLTGSTSLVFWVEVVLLVLFAVFWVIQTIELWDQGLRRP"
     gene            369..989
                     /locus_tag="PL014_11405"
     CDS             369..989
                     /locus_tag="PL014_11405"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT57741.1"
                     /translation="MARYLDVHPVDPQPRAMAQAVSVLREGGLIAYPTDSGYALGAMM
                     GNQHAKERILAIRRLDDKHHFTLVCRDFAQLGQLVHVDNAVFRAVKASTPGPYTFILP
                     GTSEVPRRLLHPKKRTVGVRIPDHPVVRALLAELGEPLLSSTLILPGEKGPMTDGWTV
                     KEELDHVVDVVIDSGECGTEPTTVVDLSSGSAEIVRVGAGDPARFE"
     gene            complement(1023..>1755)
                     /locus_tag="PL014_11410"
     CDS             complement(1023..>1755)
                     /locus_tag="PL014_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HOT57742.1"
                     /translation="GGIPTFQQFVDSTFQDEDRGFIVNGDEPATTTGDLRRFYDGMVT
                     DTATPIDGLIVNTVSGRDDKWSAAQALNLTYCVSTKFSGDYERVKTAMAEGAAQWEAA
                     SSNVNFTYVPAQDRSCTTRNSNVVFSVEPTKTTQYIARAFFPSSGKSSRNVLVNAKSL
                     YSSGSWTPSNIMAHELGHVLGLRHEHTRPEAGTCFEDNNWRPLTPYDSASIMHYPQCN
                     GTSSNLSMTSSDRTGIRALYGGTGN"
     assembly_gap    1756..1855
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1856..2955)
                     /locus_tag="PL014_11415"
     CDS             complement(<1856..2955)
                     /locus_tag="PL014_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT57743.1"
                     /translation="MQCDHFDAGRCRSCTLMGVPYDQQLADKERRVAEVLAPVAPALL
                     WVDPARSPSSHYRNKAKLVVGGTAGNPTLGILDHRGRGIDLRDCGLYEPGLAATFGPL
                     HDLVVRLGLEPYDVPARRGELKYLLVTHSPAGEQLVRFVLRSQRHLDAVRAAIPALLD
                     ELPSVRVVTANLHPEHKAVLEGDTEIALTPQTTLPMTVGDVTLRLGPRSFFQTNTTVA
                     AALYRQARDWVDAAAPTVVWDLYCGVGGFALHAAAPGRTVVGVETSADAVAAARGAVA
                     AAPDAGYVEFVVGDATTTTVEPAPDLVVVNPPRRGIGPDLAAWLDASPAGHVIYSSCN
                     VVTLARDLAAMPSLRAVTGRVFDMFPQTPHTEV"
     gene            3204..>4248
                     /locus_tag="PL014_11420"
     CDS             3204..>4248
                     /locus_tag="PL014_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007539999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT57744.1"
                     /translation="MTENTLDAAAPTLPEHDPATYRRLPARTRPVDPSVVERLLAQEW
                     ERFETWSAGSGEHNKRASESLPLGVTSSFQHWDPYPISVASARGAWLTDVDGNRMLDL
                     SMGFGAMLVGHLNPAVVAAVTDALTETGTLFVTPSPQATEMSERFCQRFQLDMIRFTN
                     SGTESLMYAIRCARAYTGRKAIVKVEGGYHGGYDGLQISVKPGLDEIGPADAPTPQVP
                     FDVEAGTVHVVAYNDLGQLTRVLEEHGSEIAAFVVEPVIENLSIVVPDAGYLAAVREL
                     CDAHGVVLIFDEVKTGLTAGAAGAAQRLGVKPDLITLAKSIGGGLPLAAFGGRREVME
                     VVVDGRMAHFGTYN"
BASE COUNT          598 a         1373 c         1524 g          653 t
ORIGIN      
        1 gcggtcacga tgacggcgag gaacatcggg atggccgcgg catcgccgtg gtgtggctca
       61 acgcccgaga cgtccgccgc ggggtgcagc agggctcgat gccttgggac cgcacgcgct
      121 acgtcgcgat gtaccgggcc gtcgccgtcg cgatgttgct cgccctcgcc gccaccgttg
      181 cgctgagcct gctgaccggg tcgacctcac tggtgttctg ggtcgaggtc gtgctgctcg
      241 tcctgttcgc cgtgttctgg gtcatccaga ccatcgagtt gtgggaccag ggtctgcgcc
      301 gtccctgagg cggtcgcgcg ggtggccacg cgacggcatc cgggccgtcc cgggcctatc
      361 gtgtcgtcat ggcccgctac ctcgacgtcc acccggtcga cccccagccg cgcgccatgg
      421 cgcaggccgt cagcgtgttg cgagagggcg ggttgatcgc gtacccgacc gactcggggt
      481 acgccctcgg ggcgatgatg ggcaaccagc acgccaagga gcgcatcctc gcgatccgcc
      541 ggctggacga caagcaccac ttcaccctcg tgtgccgcga cttcgcccag ctggggcagc
      601 tcgtgcacgt cgacaacgcg gtgttccggg ccgtcaaggc gtcgacgccg ggcccctaca
      661 ccttcatcct gccgggcacg tccgaggtgc cgcggcggct gctgcacccg aagaagcgca
      721 cggtgggcgt gcggattccc gatcacccgg tggtgcgcgc cctgctcgcc gagctcgggg
      781 agcccctgct gtcctcgacg ctcatcctgc ccggcgagaa gggtccgatg accgacggct
      841 ggacggtcaa ggaggaactc gaccacgtcg tcgacgtcgt catcgactcg ggggagtgcg
      901 gcaccgaacc gacgacggtc gtcgacctgt ccagcgggtc ggccgagatc gtgcgggtcg
      961 gggcgggaga cccggcgcgc ttcgagtgag gcgcgccggg cccggggggt gaccctcgac
     1021 ggtcagttcc cggtgccgcc gtacaacgcc cggatgccgg tgcggtcgct cgacgtcatg
     1081 ctcaggttgc tcgacgtccc gttgcactgc gggtagtgca tgatcgacgc cgagtcgtag
     1141 ggcgtcagcg ggcgccagtt gttgtcctcg aagcaggttc ccgcttcggg gcgggtgtgc
     1201 tcgtggcgca ggcccaggac gtgtcccagc tcgtgggcca tgatgttgct cggggtccac
     1261 gacccggacg agtacaggct cttggcgttg acgagcacgt tgcgcgagga cttgcccgag
     1321 ctcgggaaga acgcgcgggc gatgtactgc gtggtcttcg tcggctccac cgagaagacg
     1381 acgttgctgt tgcgcgtcgt gcaggaccgg tcctgggccg gcacgtaggt gaagttcacg
     1441 ttgctcgagg ctgcctccca ctgggccgca ccttcggcca tggccgtctt cacgcgctcg
     1501 tagtcaccgc tgaacttggt gctgacgcag taggtgaggt tcagggcctg ggcggcactc
     1561 cacttgtcgt cgcgaccgct cacggtgttg acgatgaggc cgtcgatggg ggtggctgtg
     1621 tcggtgacca tgccgtcgta gaaccggcgc aggtcacccg tggtcgtggc cggttcgtcg
     1681 ccgttgacga tgaagccgcg gtcctcgtcc tggaacgtcg agtcgacgaa ctgctggaag
     1741 gtggggatgc cgccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacctc
     1861 ggtgtgcggc gtctgcggga acatgtcgaa cacgcgcccg gtgaccgctc gcagggacgg
     1921 catcgccgcc aggtcacggg cgagggtcac cacgttgcag ctggagtaga tgacgtggcc
     1981 tgccggtgag gcgtcgagcc aggccgccag atcaggcccg atcccacgcc gaggggggtt
     2041 gaccaccacg aggtccgggg ccggctccac ggtggtcgtc gtcgcgtcgc cgacgacgaa
     2101 ctcgacgtac ccggcatccg gcgccgcggc gaccgcgcca cgggcggcgg ccacggcatc
     2161 cgcggacgtc tccacgccga ccacggtgcg accgggtgcg gcggcgtgga gggcgaaccc
     2221 accgaccccg cagtacaggt cccacacgac ggtgggcgcg gccgcgtcga cccagtcgcg
     2281 ggcctgacgg tagagcgcgg cggcgacggt ggtgttggtc tggaagaacg agcgggggcc
     2341 cagccggagg gtgacgtcac cgacggtcat cggcagcgtg gtctgcgggg tcagggcgat
     2401 ctccgtgtcc ccctcgagga cggccttgtg ctcggggtgc aggttggccg tcaccacccg
     2461 caccgacggc agctcgtcga gcagggccgg gatggcggcg cggacagcgt cgaggtgtcg
     2521 ctgtgagcga aggacgaagc gcaccagctg ttcaccggcg ggggagtggg tgacgaggag
     2581 gtacttcagc tcgccgcgcc gggcgggtac gtcgtagggc tcgagcccga ggcggaccac
     2641 gagatcgtgc agcgggccga aggtcgctgc cagaccgggt tcgtagaggc cgcagtcgcg
     2701 caggtcgatg ccgcggcccc ggtggtcgag gatcccgagg gtcgggtttc cggcggtgcc
     2761 gcccacgacg agcttggcct tgttgcggta gtgcgacgag gggctgcgag ccgggtcgac
     2821 ccagagcagc gcgggggcga cgggcgcgag cacctcggcg actcggcgct ccttgtcggc
     2881 cagctgctgg tcatagggaa cgcccatgag cgtgcaggac cggcagcgtc cggcgtcgaa
     2941 gtggtcacac tgcatggtga ccgtaagcct aggtggtggc ggtgccgagc cctaccgtgg
     3001 cagtcggcac cggcgggttc gatgcctgaa tcgagtgaac tgctgccgtt accaccggtg
     3061 atgggcgtca ccgacggctg aatccgtagg ccgatctgat gcaacgccat gggatcagta
     3121 ggtcatcggc gtcatccgac gctaggctgg cccgacaccg caccacgatc ccgccccgag
     3181 gggtggggga ggagagtcac ccaatgaccg agaacacgct ggacgcagcc gcgccgacgc
     3241 tgcccgagca cgacccggcg acctaccgtc gcctcccggc gcggacccga ccggtggatc
     3301 cttccgtcgt cgagcggttg ctcgcgcagg agtgggagcg gttcgagacg tggtcggcag
     3361 gctccgggga gcacaacaag cgggccagcg agtcgctgcc gctcggcgtg acgagctcgt
     3421 tccagcactg ggacccgtac ccgatcagcg tcgcgtctgc gcggggcgcc tggttgaccg
     3481 atgtcgacgg caaccgcatg ctcgacctgt cgatgggctt cggggccatg ctcgtcggtc
     3541 acctcaaccc cgcagtggtc gccgccgtca ccgacgcgct gacggaaacg ggcacgctgt
     3601 tcgtgacccc ctcgccccag gcgacggaga tgagtgagcg gttctgccag cgcttccagc
     3661 tcgacatgat ccggttcacc aactccggca ccgagtcgct catgtacgcc atccgctgtg
     3721 cgcgcgccta caccgggcgc aaggcgatcg tgaaggtcga gggcggctac cacggtggct
     3781 acgacggtct ccagatctcg gtcaagccgg ggctcgacga gatcggcccg gcggacgccc
     3841 cgaccccgca ggtgcccttc gacgtcgagg ccggcaccgt tcacgtcgtc gcctacaacg
     3901 acctcggcca gctgacgcgg gtccttgagg agcacggcag cgagatcgcc gcgttcgtcg
     3961 tcgagccggt catcgagaac ctgtcgatcg tcgttcccga cgccggctac ctcgcagcgg
     4021 tccgtgagct gtgtgacgcc cacggtgtcg tcctcatctt cgacgaggtc aagaccgggc
     4081 tgaccgcggg tgcggcgggg gcggcccagc gcctgggtgt gaagcccgac ctcatcacct
     4141 tggcgaagtc catcggtggc gggctgccgc tggctgcctt cggcggccgg cgtgaggtca
     4201 tggaggtcgt cgtcgacggc cggatggcgc acttcggcac ctacaacg
//
LOCUS       DAOSOC010000188         4247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_20874_length_4247_cov_10.194418, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000188 DAOSOC010000000
VERSION     DAOSOC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20874_length_4247_cov_10.194418"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PL014_11425"
     CDS             complement(<1..1488)
                     /locus_tag="PL014_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT57745.1"
                     /translation="MASTLTDTTELPTADEAIAPRPERRHPAIIQGGMGVAVSGWRLA
                     RAVSRAGQLGVISGTAMDAVIARRLQDGDAGGHYRRALEHFPSPAMAQRVLDKYFIEG
                     GRAEDAPYKPLPKLTIDTSVAAQELALVGNFAEVWLAKEGHDGVVGTNCLEKIQMANP
                     TAILGAMLAGVDYVLMGAGIPREIPQLIRALAAGRVGRIPVDVSGGTEHHYVEADPVA
                     LLGDDLPQLTRPNFLAIISLDQLASYLHRDPEIRPDGFIVERPPAGGHSAPPRGKMVL
                     DEHGAPVYGARDEADLTKMAAIGLPFWMAGAYGTSERVAAAVAAGAQGVQVGTVFALS
                     SDSGLTDEARSLLLGRLRDGELVVRNDPRASPTGFPFKVISVDGTVSEQAVYESRPRL
                     CDLSYLRQAYQREDGSIGYRCAAEPVHMFVKKGGSVDDTVGRKCLCNGLVATIGMAQH
                     RKDGYVEDRIMTLGEDLDGPRDLIARHPQGWTAAQALAYLQEGLPS"
     gene            complement(1562..2461)
                     /locus_tag="PL014_11430"
     CDS             complement(1562..2461)
                     /locus_tag="PL014_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HOT57746.1"
                     /translation="MIPALEVLETGPLVLIEDEGRPGHASVGVGHSGAADRSAHRLGA
                     RLVGNDEHLASLEVLLGGLRVRARGRLTVALTGAPAPARVDDRPIGHASLVDLAHGSV
                     LTLGMPAAGLRTYLTVRGGIDVAPVLGSRSADTLSGVGPPPVRAGDVLPVGPAGATFP
                     HVDQAAHPSHAVPWGSGDTGVAELEVLPGPRSAWVGGWGSSLGGLLGTTWHVHGDSDR
                     IGLRLSGFRVERAAPYRDVELPSEGLVRGAVQIPAGGAPVLFLADHPATGGYPVIAVL
                     TASAIDRAAQLRPGDLVRLRPSP"
     gene            complement(2458..>2831)
                     /locus_tag="PL014_11435"
     CDS             complement(2458..>2831)
                     /locus_tag="PL014_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HOT57747.1"
                     /translation="VRYDGADLAEVAAMTGLDPAEVVRAHTSTLWRVAFGGFVPGFGY
                     LVGGDPRLQVPRRESPRTRVPAGSVALAGEFTGVYPRASPGGWQLIGTTDAVLWDPDR
                     SPPAVLTPGTRVRFVDIEASR"
     assembly_gap    2832..2931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2932..3648)
                     /locus_tag="PL014_11440"
     CDS             complement(<2932..3648)
                     /locus_tag="PL014_11440"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOT57748.1"
                     /translation="MTVRIDLNADLGESFGTWVVGDDEAMLDVVTSANIACGFHAGDA
                     TTIRRTVAMAAERGVVIGAQVSYRDLAGFGRRRMDMDPHDLTADVLYQLGALEAMCRV
                     AGTRVAYVKPHGALYTTAAVDEGQARAVVDAVAAYDRTLPLLGLPGSVLLRIAEAEGL
                     TVVGEAFADRGYAATGHLVPRSHPEALHRDPDAVAERVVRLAVEGTVVSVEGEVVAVD
                     ARSVCTHGDTPDAVALARAVR"
     gene            complement(3745..3951)
                     /locus_tag="PL014_11445"
     CDS             complement(3745..3951)
                     /locus_tag="PL014_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT57749.1"
                     /translation="MATGTVKWFNADKGFGFIAPDDGSADVFAHYSAINSNGYRSLDE
                     NAKVEYETQQGAKGPQAANITVIG"
BASE COUNT          585 a         1445 c         1560 g          557 t
ORIGIN      
        1 gctgggcagg ccctcctgga ggtaggcgag cgcctgggcg gcggtccacc cctgcggatg
       61 ccgggcgatc aggtcgcgcg gaccgtcgag gtcctcgccg agggtcatga tccggtcctc
      121 gacgtacccg tccttgcggt gttgtgccat gccgatcgtc gcgacgaggc cgttgcacag
      181 gcacttccgc cccacggtgt cgtcgaccga cccacccttc ttgacgaaca tgtgcaccgg
      241 ttcggcggcg cagcggtagc cgatgctgcc gtcctcgcgc tggtaggcct ggcgcaggta
      301 cgacaggtcg cacagccgag gccgggactc gtagacggcc tgctcgctga ccgttccgtc
      361 gacggagatc accttgaacg ggaacccggt cgggctggcg cgtgggtcgt tgcgcacgac
      421 cagctcaccg tcgcgcagcc gccccagcag cagggaccgg gcctcgtcgg tcagccccga
      481 gtccgacgac agggcgaaga cggtgccgac ctgcacgccc tgggcgccgg ccgccaccgc
      541 ggcggccacc cgctcggacg tgccgtaggc gccggccatc cagaacggca ggccgatcgc
      601 ggccatcttc gtcaggtcgg cctcgtcacg agccccgtag acgggcgcgc cgtgttcgtc
      661 gaggaccatc ttcccgcgcg gcggcgcgga gtgaccgccc gccggggggc gttcgacgat
      721 gaagccgtcg gggcggatct cggggtcgcg gtgcaggtac gacgccagct ggtccagcga
      781 gatgatcgcg aggaagttcg ggcgggtgag ctgcggcagg tcgtcgccga gcagcgcgac
      841 cgggtcggcc tcgacgtagt ggtgctcggt gccccccgag acgtcgacgg ggatgcgtcc
      901 gacccgtccc gcggcgagtg cccgaatgag ctgggggatc tcgcgtggga tgcccgcgcc
      961 catgaggacg tagtcgacac cggcgagcat ggccccgagg atggccgtcg ggttggccat
     1021 ctggatcttc tcgaggcagt tcgtgcccac gacgccgtcg tgaccctcct tcgccagcca
     1081 cacctcggcg aagttgccga cgagggccag ctcctgggcg gcgaccgagg tgtcgatggt
     1141 gagcttcggc agaggcttgt acggggcgtc ctcggcgcgg ccgccctcga tgaagtactt
     1201 gtcgagcacc cgctgcgcca tcgccgggct ggggaagtgc tcgagggctc gccggtagtg
     1261 cccgcccgcg tcgccgtcct ggaggcggcg ggcgatcacg gcatccatcg ccgtccccga
     1321 gatgaccccg agctgcccgg cgcgcgagac ggctcgcgcc agtcgccacc ccgagaccgc
     1381 gacccccatc ccgccctgga tgatcgccgg gtgacgtcgt tcaggtcgtg gcgcgatggc
     1441 ctcgtcggca gtcggcagct cggtggtgtc ggtcagggtc gaggccatgg gtggctcacc
     1501 ttcggtcgtt ccgggtctgc gtcgtcgcag gtgtcgtgtc cgcaacctac ggtagcgtaa
     1561 gttagggcga agggcgcagc cggacgaggt cgccggggcg cagctgggcg gcgcgatcga
     1621 tcgcggatgc cgtgagcacc gcgatcacgg gatagccgcc ggttgcgggg tggtcggcga
     1681 ggaagagcac gggagccccg ccagcgggga tctgcaccgc gccccgcacg agcccctcgc
     1741 tgggcagctc cacgtcgcgg tagggggctg ctcgttcgac gcggaacccg ctgagccgca
     1801 gcccgatccg gtcactgtcg ccgtggacgt gccacgtcgt gccgaggagg ccgccgagcg
     1861 aggaccccca cccgccgacc cacgccgacc gtggcccggg cagcacctcg agctcggcca
     1921 ccccggtgtc gcccgagccc cagggcaccg catggctcgg gtgggcggcc tggtcgacgt
     1981 gggggaaggt ggctcccgcc gggccgacgg gcagcacgtc gccggcgcgc accgggggcg
     2041 gcccgacccc ggacaacgtg tccgccgagc gcgagccgag caccggcgcg acgtcgatgc
     2101 cgccacgcac ggtgaggtag gtgcgcaggc ccgcggccgg catccccagg gtgagcaccg
     2161 agccgtgggc gaggtcgacg aggctggcgt gcccgatcgg gcgatcgtcg acgcgggctg
     2221 gagccggggc gccggtcagc gcgacggtga ggcggccgcg ggcccgcacg cgcaggccgc
     2281 cgagcagcac ctcgagggac gcgaggtgtt cgtcgttccc gacgaggcgg gcgcccagtc
     2341 ggtgtgccga gcggtcggcg gcgccggagt ggccgactcc gacgctggcg tggccgggtc
     2401 ggccctcgtc ctcgatgagc acgagcgggc cggtttccag gacctcgaga gccgggatca
     2461 ccgcgacgcc tcgatgtcga cgaagcgcac ccgggtgccg ggggtgagca ccgccggtgg
     2521 gctgcgatcg gggtcccaga gaacggcatc cgtcgtgccg atgagctgcc acccgccggg
     2581 ggaggcccgc gggtagacac cggtgaactc cccggccagc gcgaccgatc cggcgggcac
     2641 ccgggtgcgc ggggactcgc ggcggggtac ctggaggcgt ggatcgccgc cgacgaggta
     2701 gccgaagccc gggacgaagc caccgaaggc cactcgccac agggtgctcg tgtgggcccg
     2761 cacgacctcg gcagggtcga ggccggtcat cgccgcgacc tccgcgaggt cggcgccgtc
     2821 gtagcgcacg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgcacggc
     2941 gcgggccagc gcgacggcat caggggtgtc gccgtgcgtg cagaccgacc gcgcgtccac
     3001 ggcgacgacc tcgccctcga cggagacgac ggtgccctcg acggcgaggc ggacgacccg
     3061 ctcggccacg gcgtccgggt cacggtgcag ggcctcgggg tgcgagcgcg gcaccaggtg
     3121 accggtcgcc gcgtacccgc ggtcggcgaa ggcctcgccc acgacggtca gcccctcggc
     3181 ctcggcgatg cgcagcagca ccgagcccgg caggcccagc agggggaggg tgcggtcgta
     3241 ggcggccacg gcatccacga cggcccgggc ctgaccctcg tcgacggcgg ccgtggtgta
     3301 cagggcgccg tggggcttga cgtacgcgac gcgggtcccc gccacccggc acatcgcctc
     3361 gagggcaccg agctggtaga gcacgtcggc ggtgaggtcg tgcgggtcca tgtccatgcg
     3421 gcggcgcccg aagccggcca ggtcgcggta ggagacctgc gccccgatga ccaccccccg
     3481 ctcggcggcc atggccacgg tgcggcggat cgtcgtggcg tcgccggcgt ggaagccgca
     3541 cgcgatgttg gcgctggtca ccacgtcgag catcgcctcg tcgtcaccga ccacccaggt
     3601 gccgaaggac tccccgaggt cggcgttgag gtcgatccgc acggtcatgc ccgaaggcta
     3661 gtgtgcgccg gacatgacac gaccgccgcc cggttgcccg gacggcggtc gtgtgaggtg
     3721 ccccgagggg caacaggtgg aacgtcagcc gatgacggtg atgttcgcgg cctgcgggcc
     3781 cttcgcgccc tgctgggtct cgtactcgac cttggcgttc tcgtcgagcg aacggtagcc
     3841 gttcgagttg atcgccgagt agtgggcgaa gacgtcggcg gagccgtcgt cgggggcgat
     3901 gaagccgaag cccttgtcgg cgttgaacca cttcacggtg ccagtagcca taggaaggcc
     3961 tactttctga gagaggagta ccacccgctt gtcgggggac tccgcgttgg tgatgcctgt
     4021 agtacctctg ctcagaaagt gctctgtccc ggcgggacgt tctgccacta cttcacaacg
     4081 gaaagctcaa caactggtga caacactacc ggctaccggc gagttcacca caaccacgtg
     4141 ccgcagtggt cgatccagcc ttcgtgggct ggcccgatga cgaccaccag aggagacggc
     4201 cagacccccc tgacccgcct gacgaccaac tccgtgggca cccacgg
//
LOCUS       DAOSOC010000189         4244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_20897_length_4244_cov_8.614705, whole genome shotgun sequence.
ACCESSION   DAOSOC010000189 DAOSOC010000000
VERSION     DAOSOC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20897_length_4244_cov_8.614705"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PL014_11450"
     CDS             complement(<1..435)
                     /locus_tag="PL014_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT57750.1"
                     /translation="MPPIDRSRLTDLIAAEQEVYTAAHPRSRELFAQGSNLFGRVPMT
                     WMNKWTGGFPLYLDHARGNRISDVDGHTYVDFALGDTGAFAGHSPRPTVEAVARRMGD
                     LGGITTMLPSADAQWVGAELTARFTMPLWSFTLSATDANRWAV"
     gene            631..1386
                     /locus_tag="PL014_11455"
     CDS             631..1386
                     /locus_tag="PL014_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOT57751.1"
                     /translation="MARQRTPEGPPPAPAVQKLRIRYAKRGRLRFSSTRDFSRALERA
                     LRRADVPMAFSAGFHPHPLISYANAAPTGTASEAEYFEIRVTERVEPEAVRAALDEAL
                     PEGLDVLEVVQAGPGALADRLEASEWHIVVRAADPAEVTAAVDAYLACEVAETTRTFK
                     TGPRTFDTRSAVLAMDASGVDDEGCAILRMVVRHTTPSVRPDDILTALHAVTGFSPPV
                     PPLVTRLAQGPLLDAAAGVADPLAADKEAVGSG"
     gene            1549..>4244
                     /locus_tag="PL014_11460"
     CDS             1549..>4244
                     /locus_tag="PL014_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOT57752.1"
                     /translation="MASENDSTTTLDTAAGEQAPAEKPKRRRATKKAAAPAAVDPAAA
                     AESAADATDAVAADAGEVAPAPVKRAPRKRAAKKAAPAAQGTDLAPSAADGEAAAPAD
                     VADSAEGTDGDAPAPAEAPKARRATKAAKSPRVSKRAKATKAPAEVLLDDLSLLEPGT
                     ADLLLPIDEDESEAVAQAPDAEAAAPADDESPLPAVPSLSMLFQAPEPVAPRRRRATA
                     PVQAPVERPAVDDTAADSVSTSADADAADARDDVAAADDAAAADAGQGESATTDRASE
                     DADTVGRGRRRRGGRGRRGRSQGEDDKGATASADAPDAEQPSGAGPEDTGADTEGESV
                     SSRRRRRRRRGAGSGDGEGDDPPGTVTRVREPRGQRDEPTAVKGSTRLEAKKQRRREG
                     REAGRRRTIITEAEFLARRESVERVMVVRGRGDRTQIGVLEDGVLVEHYVSRETTSSM
                     AGNVYLGRVQNVLPSMEAAFVDIGKGRNAVLYAGEVNWDAAGLEGGQARRIENALSSG
                     DTVLVQVTKDPIGHKGARLTSQISLPGRYLVYVPEGQMTGISRKLPDTERARLKAILK
                     EVVPDHAGVIVRTAAEGASEEELRADVERLTKIWDKIQAKAESAGAKKAGSGGAPALL
                     HGEPDLTVRVIRDVFTEDFTSLVVSGDKAWSEVSQYVEDVAPDLAPRLQRWTSEADVF
                     AAHRIDEAIAKAMDRKVWLPSGGSLVIDRTEAMTVVDVNTGKFIGAGGNLEETVTKNN
                     LEAAEEIVRQLRLRDIGGIIVVDFIDMVLESNRDLVVRRLLECLGRDRTKHQVAEVTS
                     LGLVQMTRKRVGSGLVEVFSETCEHCSGRGIVVHSEPIDRAGANDVGGANAAPSRSGR
                     GRRGTPAAGGSSNGGSDAVAQVEEVRTPSGPTAAQIAAAAHAA"
BASE COUNT          630 a         1551 c         1533 g          530 t
ORIGIN      
        1 caccgcccag cggttcgcgt cggtcgccga gagcgtgaag ctccacagcg gcatcgtgaa
       61 gcgtgcggtc agctcggccc cgacccactg ggcgtcagca ctcggcagca tcgtcgtgat
      121 gccgccgagg tcgcccatgc ggcgcgccac cgcttcgacg gtcgggcggg gggagtgccc
      181 ggcgaacgct ccggtgtcac cgagcgcgaa gtccacgtag gtgtggccgt cgacgtcgga
      241 gatccggttg ccgcgggcgt ggtcgaggta gagcgggaag ccgccggtcc acttgttcat
      301 ccacgtcatc gggacacggc cgaacaggtt gctgccctgc gcgaagagct cgcggctgcg
      361 cgggtgggct gcggtgtaga cctcctgctc ggcggcgatg aggtcggtca ggcgagagcg
      421 gtcgatcggt ggcatgcgag cacgatagcg gactgaatca gtggtcaatc actgttcgta
      481 ggccgaaaac ggcatcggat gccgtggcgc acgacggatt tcggagctcg cccaggggtg
      541 gggctgatcg tgaacagctg ccgaccctgc cgcgggcgat ggtcgagccc atccggccga
      601 ggccgcccag ccgtctacgc tgggggcacc atggctcgac agcgcacccc tgaggggccc
      661 ccacccgcac ccgccgtgca gaagctgcgc atccgctacg ccaagcgtgg ccggttgcgc
      721 ttctcgtcca cgcgtgactt ctcccgggcc ctggagcgcg cgcttcgccg ggccgacgtc
      781 ccgatggcgt tctcggccgg gttccacccg cacccgctca tctcctacgc caacgcggcg
      841 cccacgggca cggcgagcga ggccgagtac ttcgagatcc gcgtcaccga acgggtcgag
      901 ccggaggcgg tgcgggccgc cctcgacgag gcgctgcctg aggggctcga cgtcctcgag
      961 gtcgtgcagg ctggccccgg tgcgctcgcc gaccggctcg aggccagcga gtggcacatc
     1021 gtcgtgcggg cagccgaccc tgccgaggtc accgcggccg tcgacgcgta cctggcctgc
     1081 gaggtcgcgg agacgacacg aaccttcaag accggcccgc gcacgttcga cacgcgcagc
     1141 gccgtgctgg cgatggatgc ctccggtgtc gacgacgagg ggtgtgcgat actgcgcatg
     1201 gtcgttcggc acaccacacc gtccgttcga cccgatgaca tcctgaccgc actgcacgca
     1261 gtgactgggt tctcgcctcc ggtcccaccc ctggtgactc ggctggccca aggcccactg
     1321 ctcgacgcag ctgctggggt ggccgacccg ttggctgccg acaaggaggc cgtcggttcc
     1381 ggataggtgc cgcgccgccc gcgcgagccg ctccggggca gcagctgact tccgtgcagg
     1441 gcacgcccgg cacgatccga tcgcaccacg cggtcggcga gggaccggtc aggagttggg
     1501 gccggaaccg gtgtggtcgt cgcccacgtg gatgacggaa ggggcctcat ggcttccgag
     1561 aacgactcca ccacgacgct tgacacggcc gccggtgaac aggcgcccgc cgagaagccc
     1621 aagcgccgcc gcgccacgaa gaaggcggcc gcgccggccg ccgtcgatcc ggctgctgcc
     1681 gccgagagcg ccgcggacgc cacggatgcc gtggccgccg acgccggcga ggtcgccccc
     1741 gcgcccgtca agcgcgcccc gcgcaagcgg gcggcgaaga aggccgcccc ggcagcccag
     1801 ggcaccgacc tcgcgccgag cgctgccgat ggggaggccg ccgcacccgc cgacgtggcc
     1861 gattccgctg agggcacgga cggtgacgcc ccggcacccg ccgaggcgcc caaggccagg
     1921 cgcgcgacca aggccgcgaa gtcaccccgg gtcagcaagc gcgccaaggc gaccaaggcc
     1981 ccggccgagg tcctgctcga cgacctctcc ctgctcgagc ccggcacggc cgacctgctc
     2041 ctgcccatcg acgaggacga gtccgaggcc gtggcgcagg cgccagacgc cgaggccgct
     2101 gctcccgccg acgacgagtc gcccctgccc gcggtcccga gcctgtccat gctcttccag
     2161 gcccctgagc cggtcgcccc gcgccgccgc cgggccaccg ctccggtgca ggccccggtc
     2221 gagcgccccg cggtcgacga caccgctgcc gactccgtgt cgacctccgc agacgccgac
     2281 gcagccgacg cacgcgacga cgtggccgcg gccgacgacg ccgccgcagc cgacgcgggc
     2341 cagggcgagt ccgccacgac cgaccgcgcc tccgaggacg cagacaccgt cggccgtgga
     2401 cgccgtcgcc gtggcggtcg aggccgccgt gggcgaagcc agggtgagga cgacaagggg
     2461 gccacggcat ccgccgacgc ccccgacgcc gagcagccca gcggcgccgg gcccgaggac
     2521 accggggctg acaccgaggg cgagtcggtc tcgagccggc gtcgtcgtcg gcgccgccgc
     2581 ggcgcaggca gtggtgacgg cgagggcgac gacccgcccg gaaccgtgac ccgggtgcgc
     2641 gagccccgtg gccagcggga tgagcccacc gccgtcaagg gctccacgcg cctcgaggcc
     2701 aagaagcagc gtcgtcgcga gggccgtgag gccgggcgtc gccgcacgat catcaccgag
     2761 gccgagttcc tggcccgtcg cgagagcgtc gagcgggtca tggtcgtgcg cggccgtggt
     2821 gaccgcaccc agatcggcgt gctcgaggac ggcgtgctcg tcgagcacta cgtctcgcgt
     2881 gagaccacgt cgtcgatggc tggcaatgtc tacctcggcc gggtgcagaa cgtgctgccg
     2941 tcgatggagg ccgcgttcgt cgacatcggc aagggccgca acgccgtgct ctacgccggt
     3001 gaggtcaact gggacgccgc cgggctcgag ggcggccagg ctcgccgcat cgagaacgcg
     3061 ctgtcctcgg gcgacaccgt gctcgtccag gtcaccaagg acccgatcgg gcacaagggc
     3121 gctcgcctga cctcgcagat cagcctgccc gggcgctacc tcgtctacgt gcccgagggg
     3181 cagatgacgg gcatctcccg caagcttccc gacaccgagc gggcgcgcct gaaggccatc
     3241 ctcaaggagg tcgtgcccga ccacgccggt gtcatcgtgc gcaccgccgc cgagggggcc
     3301 tccgaggagg agctgcgcgc cgacgtcgag cgcctcacga agatctggga caagatccag
     3361 gccaaggccg agtcggccgg ggccaagaag gccggcagtg gcggcgctcc cgccctgctg
     3421 cacggcgagc ccgacctcac cgtgcgcgtc atccgcgacg tcttcaccga ggacttcacc
     3481 tcgttggtcg tctccggcga caaggcgtgg tcagaggtca gccagtacgt cgaggacgtc
     3541 gcccccgacc tcgcaccgcg cctgcagcgc tggacctccg aggccgacgt gttcgcggcg
     3601 caccgcatcg acgaggcgat tgccaaggcg atggaccgca aggtgtggct gccctcgggt
     3661 gggtcgctcg tgatcgaccg caccgaggcg atgaccgtcg tcgacgtcaa caccggcaag
     3721 ttcatcggtg ccgggggcaa cctcgaggag acggtcacca agaacaacct cgaggccgct
     3781 gaggagatcg tccgccagct gcgactgcgc gacatcggcg gcatcatcgt cgtcgacttc
     3841 atcgacatgg tgctggagtc caaccgcgac ctcgtcgtgc gacggctcct ggagtgcctg
     3901 ggccgtgacc gcacgaagca ccaggtggct gaggtcacct cgctcggcct cgtgcagatg
     3961 acccgcaagc gggtcgggtc gggcctggtc gaggtcttct cggagacgtg cgagcactgc
     4021 tccgggcgcg gcatcgtcgt gcactccgag ccgatcgacc gggccggcgc gaacgacgtc
     4081 ggcggggcca acgcagcgcc gtcgcgatcg ggtcgcggcc gtcgggggac cccggctgcc
     4141 ggtggttcgt cgaacggcgg gtcggatgcc gtggcgcagg tcgaggaggt ccgcaccccg
     4201 tcgggtccca cggccgccca gatcgccgct gcggcgcacg cggc
//
LOCUS       DAOSOC010000190         4227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_21020_length_4227_cov_8.784276, whole genome shotgun sequence.
ACCESSION   DAOSOC010000190 DAOSOC010000000
VERSION     DAOSOC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21020_length_4227_cov_8.784276"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /gene="mshA"
                     /locus_tag="PL014_11465"
     CDS             <1..1061
                     /gene="mshA"
                     /locus_tag="PL014_11465"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HOT57753.1"
                     /translation="SKEDLPGQLCAFAAGMMRVAAHAPPGYYDLVHSHYWLSGQVGWL
                     AADRWGVPLVHTMHTMARVKNLHLADGDEPEPRGREIGEAQVVEAADRLVANTPREAA
                     ELVELYGADPARVAVAEPGVDLTVFSPGSKAEARARVGIPADAVLLLFVGRIQPLKAP
                     DVLVRAAAELVRRRPDLRSRLVVGILGGASGTGVRNPMGLTELAQQLGIADLVRFVPP
                     VDRSTLAQWYRAADLVAVPSHSESFGLVAVEAQACGTPVVAANVGGLPTAVGPAGVLV
                     DGHDTADWATALEAVLRDPRGAAALARTSVEHAAGFSWARTAGRLAEVYADAMDHPRE
                     VPIHDAELLIGIPTAVIP"
     gene            1076..1585
                     /locus_tag="PL014_11470"
     CDS             1076..1585
                     /locus_tag="PL014_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOT57754.1"
                     /translation="MRATIEAFLAASGLEWEPGARDDEYVVALPGEKKLKTITSLVVS
                     DTALSVSAFVIRNPDENHAEVYRFLLRRNLRLPGLAYSVDKAGDVFVTGRLPAAGVDV
                     DSLDQLLGVVLTACDEPFNELLVMGFLSSMRKEWEWRVSRGESLRNLEAFRGILARPG
                     DPDPGSAPT"
     gene            1618..2355
                     /locus_tag="PL014_11475"
     CDS             1618..2355
                     /locus_tag="PL014_11475"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HOT57755.1"
                     /translation="MTEHTLILLRHGESDWNQKNLFTGWVDVDLTDKGRAEATRGGHL
                     MKDAGILPDVVHTSVQRRAITTANLALDAADRHWIPVHRSWRLNERHYGALQGLDKAA
                     TREKYGDEQFMLWRRSFDTPPPPIEVGSEFDQTNDPRYAGIKVPLTECLKDVIPRMMP
                     YWENEIRSDLAEGRTVLVTAHGNSLRALVKHLDGISDDDIAGLNIPTGMPLVYRLGDD
                     FMPTGPGEYLDPEAAAAAAAAVANQGR"
     gene            complement(2358..>4227)
                     /locus_tag="PL014_11480"
     CDS             complement(2358..>4227)
                     /locus_tag="PL014_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57756.1"
                     /translation="DRASRVTADALARLNRTWRDVVDAGRPGEQARAAVLHAALNTAG
                     AATPTSSPGTGPTTRSDVSHEAVRDAGTTADPVTTALIDAIRSAPPIERALLATHHVW
                     DLDPDAVSHLLQQTSTRGRATRDTDPATLTDHAASLDARLTAAHDAAHLADGRDPSPW
                     RRERDVSTAIDHLLRDTHDPPDPAALVATRTTGIRRRTLLTAGATTLALTAAGAALVR
                     RGSADAPSMSTTTTARLAPEDPRWASATQWTPRGPLADDAAVIALALRSGDPRNRLLW
                     AGDIGTQRAVLILVHTSPGNDVDGADPPEPGVTYSEPFISVYQGPRGLDPARLTPRTL
                     VTSGIVTSGEDGDVVAVVLQPDPEGPAQAPLVVLARPTVQSASLSQALRPRADGTIER
                     DWAPLPLDGGVGTLTLATGLPLATRLRVGSWEGTPESFTEIYTAISEGPVPSTSPEWA
                     HRCITAITGIPAADLTSTVFVDAEIPRGLFDWPPPPDVVPARLVSLLTTTPEGGVFRT
                     TAYTGDGFTWALEPAQVFSRENARDPLVVWASDERVGVARFLVAAPGAAQVQLISTSP
                     DGYPVSKVVPTRGQDAVIVPVVNGADAAEYRLVTKDADGRVLFDGVPDQGRHPTDG"
BASE COUNT          585 a         1440 c         1613 g          589 t
ORIGIN      
        1 tgtccaagga ggacctgccc ggtcagctgt gcgccttcgc ggccggcatg atgcgcgtcg
       61 ccgcccacgc gccgccgggg tactacgacc tcgtgcactc gcactactgg ctctctggac
      121 aggtcgggtg gctggccgcc gatcggtggg gcgttcccct ggtccacacc atgcacacga
      181 tggcgcgggt gaagaacctg cacctcgcgg acggtgacga gcccgaaccg cgcggccgtg
      241 agatcggtga ggcgcaggtc gtcgaggctg ctgaccggct cgtcgcgaac accccgcgag
      301 aggcggccga gctcgtcgag ctgtacggtg cggatcctgc ccgcgtcgcc gtggccgagc
      361 cgggtgtcga cctcaccgtg ttcagccccg gctccaaggc cgaggcgcga gcgcgcgtcg
      421 ggattcctgc ggatgccgtg ctcctcctct tcgtcgggcg gatccagccg ctgaaggcgc
      481 ccgacgtcct cgtgcgcgcc gccgccgagc tggtgcggcg ccggcccgac ctgcgctccc
      541 gtctcgtcgt cggcatcctc ggcggggcca gcggtaccgg ggtgcgcaac cccatgggct
      601 tgaccgagct ggcccagcag ctcggcatcg ccgacctcgt ccgcttcgtg ccacccgtcg
      661 accggtcgac cctggcgcag tggtaccgcg ccgcggacct cgtggcggtg ccctcgcaca
      721 gcgagtcctt cgggctggtc gcggtggagg cccaggcgtg cggcacaccc gtcgtggccg
      781 ccaacgtcgg tggcctgccc acggccgtcg gtcctgcggg ggtcctcgtc gacgggcacg
      841 acacggccga ctgggcgacc gccctcgagg cggtgctccg cgacccccgc ggtgctgcgg
      901 cgctggcccg caccagcgtc gagcacgcgg ccgggttctc gtgggcgcga acggcagggc
      961 gcctggcgga ggtctacgcc gacgcgatgg accacccgcg agaggtgccg atccacgacg
     1021 ccgagctgct catcggcatc cccacggcgg tgatcccgtg acgggcaggg acgagatgcg
     1081 ggccacgatc gaggcgttcc tcgcggcatc cggtctggag tgggaacccg gagcccgcga
     1141 cgacgagtac gtcgtggcgc tgcctggcga gaagaagctg aagacgatca cctcgctcgt
     1201 cgtctcggac acggcactgt cggtgtcggc gttcgtcatc cgcaaccccg acgagaacca
     1261 cgccgaggtc taccgcttcc tcctgcgccg aaacctgcgg ctgccggggc tggcctactc
     1321 ggtcgacaag gcgggcgacg tgttcgtcac cggccggctg ccggctgcgg gcgtcgacgt
     1381 cgactccctc gaccagctgc tcggagtggt gctgaccgcg tgcgacgagc cgttcaacga
     1441 gctgctcgtc atgggcttcc tgtcctcgat gcgcaaggag tgggagtggc gggtctcgcg
     1501 cggtgagagc ctgcgcaacc tcgaggcctt ccgcggcatc ctcgccagac ccggcgaccc
     1561 cgacccggga tccgcgccca cctgaccctg acgggcgcgg gtggctaggc tggcgacatg
     1621 accgaacaca cgctcatcct cctgcgccac ggcgagtccg actggaacca gaagaacctg
     1681 ttcaccgggt gggtcgacgt cgacctcacc gacaaggggc gcgccgaggc cactcgcggt
     1741 ggtcacctga tgaaggatgc cgggatcctg cctgacgtcg tgcacacctc ggtgcagcgt
     1801 cgcgcgatca cgacggccaa cctcgcgctc gacgccgctg accggcactg gatcccggtg
     1861 caccgcagct ggcgcctcaa cgagcggcac tacggcgcgc tgcaggggct ggacaaggcg
     1921 gcgacgcgcg agaagtacgg cgacgagcag ttcatgctct ggcgtcgcag cttcgacacc
     1981 ccgccgccgc ccatcgaggt cggcagcgag ttcgaccaga cgaacgaccc gcgctacgcc
     2041 ggcatcaagg tgccgctgac cgagtgcctc aaggacgtca tcccgcggat gatgccgtac
     2101 tgggagaacg agatccgcag cgacctcgct gaaggcagga cggtgctcgt caccgcccac
     2161 ggcaactcgc tgcgcgccct ggtcaagcac ctcgacggca tcagcgacga cgacatcgcc
     2221 ggcctgaaca tcccgaccgg gatgccgctc gtctaccgcc tcggcgatga cttcatgccg
     2281 accggcccgg gcgagtacct cgaccccgag gcggcagcgg cggcagcagc agcggtggcc
     2341 aaccagggtc gctgagctca gccgtccgtc gggtgccgac cctggtccgg tacgccgtcg
     2401 aagagcaccc gcccgtcggc atccttggtg acgaggcggt actcggccgc gtcggccccg
     2461 ttcacgacgg ggacgatgac cgcgtcctga ccgcgcgtcg gaaccacctt ggacaccggg
     2521 tagccgtcgg ggctggtcga gatgagctgc acctgggcgg cccccggcgc cgcgacgagg
     2581 aagcgcgcca ccccgacccg ctcgtcggag gcccacacga cgagcgggtc gcgcgcgttc
     2641 tcgcgcgaga acacctgggc cggctccagg gcccaggtga acccgtcgcc ggtgtacgcc
     2701 gtcgtgcgga agaccccacc ctccggcgtc gtggtgagca gcgacaccag acgcgccggc
     2761 acgacatccg gaggtggcgg ccagtcgaag agcccgcgag ggatctccgc gtccacgaac
     2821 accgtgctcg tgaggtcggc agcggggatg cccgtgatcg cagtgatgca ccggtgtgcc
     2881 cactccggtg acgtgctcgg gacggggcct tcggagatgg ccgtgtagat ctcggtgaag
     2941 gactcgggtg ttccctccca cgaacccacg cgcaggcggg tcgccagcgg caggccggtg
     3001 gccagggtca gggtgccgac gccgccatcc agcggcagcg gagcccagtc gcgctcgatg
     3061 gtgccgtcgg cgcggggtcg aagcgcctgc gacagcgacg cggactgcac cgtggggcgg
     3121 gccagcacca ccaggggcgc ctgcgccggg ccctccgggt cgggctgcag cacgacggcg
     3181 acgacgtccc cgtcctcgcc ggaggtgacg atgccgctgg tgaccagagt tcgcggtgtc
     3241 aggcgggccg ggtcaagacc gcgaggcccc tggtacacgc tgatgaaggg ttcggagtac
     3301 gtcacgcccg gctccggggg atcggcgccg tcgacgtcgt tgccggggga ggtgtggacg
     3361 aggatcagca cggctcgttg ggtgccgatg tcaccggccc acagcaaccg gtttcgtggg
     3421 tcgccagacc gcagggccag cgcgatgacc gcggcgtcgt cggccagagg gccacggggt
     3481 gtccactgcg tcgcagaagc ccaccgaggg tcctcagggg ccaggcgggc cgtggtggtc
     3541 gtggacatcg agggagcgtc ggcgctgccg cggcgcacca gagcggcgcc ggcggcagtg
     3601 agggcgaggg tggtggcacc tgcggtgagg agggtgcgtc gtcggatgcc ggtcgtgcgg
     3661 gtggcgacga gggcggcggg gtctggtggg tcgtgggtgt cgcgcaggag gtggtcgatg
     3721 gcggtgctga cgtcgcgttc acgacgccac ggggaggggt cgcgaccatc ggcgaggtgt
     3781 gcggcgtcgt gggctgcggt gagccgcgcg tcgagggatg ccgcgtggtc ggtgagggtg
     3841 gcggggtcgg tgtcgcgggt ggctcggccg cgggtggagg tctgctggag aaggtgggag
     3901 acggcgtcgg ggtcgaggtc ccacacgtgg tgggtggcca ggagggcgcg ctcgatcggt
     3961 ggtgcggagc ggatggcgtc tatgagggcg gtggtgacgg ggtcggcggt ggtacctgcg
     4021 tcgcgcaccg cctcgtggga cacatccgac ctagtggtcg gacccgtccc gggggaggag
     4081 gtgggggtgg cggcaccagc ggtgttgagg gctgcgtgca ggacggcggc gcgggcctgc
     4141 tcaccggggc gcccggcatc cacgacgtcg cgccaggtgc ggttgagccg tgccagggcg
     4201 tcggcggtga ccctgctggc gcggtcg
//
LOCUS       DAOSOC010000191         4204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_21181_length_4204_cov_7.561581, whole genome shotgun sequence.
ACCESSION   DAOSOC010000191 DAOSOC010000000
VERSION     DAOSOC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21181_length_4204_cov_7.561581"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PL014_11485"
     CDS             complement(<1..934)
                     /locus_tag="PL014_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOT57757.1"
                     /translation="MAHYQAMGSIPPKRHTQHRRPAKRGQVGELYSEELMGEEGFSSD
                     SSLLYHRNIPSAVAEYRVWNVGDLATTPNHPLLPLHLKPHDLFPASVVKDTDVVTGRR
                     LLLGNGDVRLSYAVAGAPSPWYRNGIGDECVYVERGRARVETVFGAFEVGEGDYVILP
                     RATTHRWIPKKSAKDPLRTIVIEASSHIAPPKRYLSKYGQFLEHSPFCERDLRGPQGP
                     LLAEDIGEDPDAHTEVYIKHRGGGPASSGGIVGTVHVVPFHPLDVVGWDGCLYPYVFN
                     VRDYEPITGRIHQPPPAHQVFEGWNFVICNFVPRK"
     gene            1017..1886
                     /locus_tag="PL014_11490"
     CDS             1017..1886
                     /locus_tag="PL014_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57758.1"
                     /translation="MPSTTSSVFRDLIDDAAVFPPGNATLPDAVAHHRAHLTSGYAGC
                     VGPLLVPAASAADLVSVLDGEPTTDAGRAASEPFEVVLIARPGADPALVPAARDLLLD
                     DPRVQVAGAEVGWFDGWVQALAGALPLAVEIPRGADQPRAITEIRAACEAGHPVVAKF
                     RTGPTPAWAWPDEAELAEVLRSMAPQVPFKLTGGLHHAARGTYEVGGVAEENHGILNV
                     LLATAAALDGAGHDEVAGLLAVRDAQALAALVGAWTPETATRVRAAFTAYGCCTVTDP
                     IDELTALNLLTKD"
     gene            1890..3116
                     /gene="fahA"
                     /locus_tag="PL014_11495"
     CDS             1890..3116
                     /gene="fahA"
                     /locus_tag="PL014_11495"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HOT57759.1"
                     /translation="MTWLDLPADHPYGIHNLPYGVFSTDGAQPRVGVRVGEHVLDVAA
                     CAEQAGMESGSVWRAGSLNPFLAQGRSAWTAAREWLTEQLTNEAHRDCVESHLVPVAE
                     VTMHLPIEVADYVDFYASEHHATNVGRMFRPDSEPLTPNWKHLPIGYHGRSGTVVVSG
                     TDIVRPCGQRKPPTEPAPVFGPSARLDIEAELGFVVGGASRLGEPVPLADAGEHLFGV
                     VILNDWSARDLQAWEYVPLGPFLGKSFATSISPWVVTMEALGAAHVRLPGQDPEPLPY
                     LRGEPTADGAGTAYGLDISLEVEWNGTVVARPQFRDMYWSPAQMLAHMTVNGASVRTG
                     DLFGSGTISGEQKDTRGSFLELSWSGTEPITLADGSQRTFLEDGDTVTLRAVAPGPDG
                     AVIGLGECTGTVLAAR"
     gene            complement(3148..4035)
                     /locus_tag="PL014_11500"
     CDS             complement(3148..4035)
                     /locus_tag="PL014_11500"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOT57760.1"
                     /translation="MSETPSGPGWYDDPDDASQLRYFDGVVWSSHTTPRKSPTIDDSV
                     IGRGAQQPDGAGLQGGWVHPGATQPGAAQQGGPQGPGQGGWSGPGYGGGQGYGAGPAY
                     GGYGAAYAQRRDVLPDGAVLAQWWRRLLARILDSFITSIVALVVAIPWLGPMFAAVTD
                     FVDATVAAAEAGGAMPDQNAYITEMLTASLPITIVSLFVGLLYETVFLVWRGATPGKM
                     ALGTVVRRVGSPGKLTVVDAIKRQLIGLATAAVGVLPVVGLLGSIVSILDPAWLLWDP
                     KRQTLHDKLADTVVVLKAR"
BASE COUNT          589 a         1512 c         1489 g          614 t
ORIGIN      
        1 ccttgcgcgg cacgaagttg cagatgacga agttccagcc ctcgaagacc tggtgcgccg
       61 gcggtggctg gtggatgcgc ccggtgatcg gctcgtagtc gcgcacgttg aacacgtacg
      121 ggtagaggca gccgtcccag ccgacgacgt cgagcgggtg gaacggcacg acgtgcacgg
      181 tgccgacgat gccgccgctg ctcgccgggc cacctccgcg gtgcttgatg tagacctcgg
      241 tgtgcgcgtc ggggtcctca ccgatgtcct cggcgagcag cgggccctgc ggaccacgca
      301 ggtcccgctc gcagaacggg ctgtgctcga ggaactggcc gtacttgctc aggtagcgct
      361 tcggcggagc gatgtggctg ctcgcctcga tgacgatggt gcgcagcggg tccttggcgg
      421 acttcttggg gatccaccgg tgcgtcgtcg cccgcggcag gatgacgtag tcaccctccc
      481 ccacctcgaa cgcgccgaac acggtctcga cacgggcccg gccacgctcg acgtacacgc
      541 actcgtcgcc gatgccgttg cggtaccacg ggctcggggc gcccgcgacg gcatacgaca
      601 gtcgcacgtc gccgttgccg agcaggagtc gacggccggt gacgacgtcg gtgtccttga
      661 ccactgacgc ggggaacagg tcgtggggct tcaggtgcag cggcagcagc gggtggttgg
      721 gcgtcgtcgc gaggtcaccg acgttccaga cccggtactc ggccaccgcc gaggggatgt
      781 tgcggtggta gagcaggctg gagtccgagg agaacccctc ctcacccatg agctcctcgg
      841 agtacagctc acccacctgg ccgcgctttg cgggccgccg gtgctgggtg tgccgcttcg
      901 gggggatgct gcccattgcc tggtagtgcg ccactgctgc cgtccactcc ttcgaggtcc
      961 ctggtgtctc ccccgcaggc tacgcccggt cagggcggct cggtaccgtg agcaccatgc
     1021 cctcgacgac cagcagcgtg ttccgcgacc tgatcgacga cgccgccgtg ttccctcccg
     1081 ggaacgccac actgccggat gccgtggcgc accaccgcgc ccacctcacc tccgggtacg
     1141 ccggctgtgt gggacccctg ctcgtcccgg ctgccagtgc tgccgacctg gtgtccgttc
     1201 tcgacggcga gccgaccacc gatgctgggc gggcggcatc agagccgttt gaggtcgtgc
     1261 tcatcgcgcg ccccggtgcg gaccccgcgc tggtgccggc ggcccgggac ctgctgctcg
     1321 acgacccacg agtgcaggtc gccggcgccg aggtcgggtg gttcgacggg tgggtgcagg
     1381 ccctcgccgg agcgctgccg ctcgccgtcg agatcccccg gggtgccgat cagccgcggg
     1441 ccatcaccga gatccgtgcc gcgtgcgagg ccggccaccc cgtcgtcgcg aagttccgca
     1501 ccgggccgac gccggcctgg gcgtggccgg acgaggccga gctggccgag gtgctgcgct
     1561 ccatggcgcc ccaggtgcct ttcaagctca ccgggggcct gcaccatgcc gctcgaggca
     1621 cctacgaggt ggggggtgtg gccgaggaga accacggcat cctcaacgtc ctgctcgcga
     1681 cggctgccgc gctcgacggc gccgggcacg acgaggtcgc cgggctgctc gccgtgcgcg
     1741 atgcgcaggc cctggccgcc ctcgtcggcg cgtggacgcc cgagacggcg acgcgggtgc
     1801 gcgccgcctt cacggcctac ggttgctgca ccgtgaccga cccgatcgac gaactgaccg
     1861 ccctgaacct gctgacgaag gactgagcga tgacctggct cgacctgccc gccgaccacc
     1921 cgtacggcat ccacaacctc ccctacggcg tcttctcgac cgacggcgct cagccacgcg
     1981 tgggggttcg cgtgggcgag cacgtgctcg acgtcgccgc gtgcgccgag caggcgggca
     2041 tggagtccgg ctcggtgtgg cgggcgggct cgctcaaccc gttcctcgcc cagggccggt
     2101 ccgcgtggac ggcggcccgc gagtggctca ccgagcagct gaccaacgag gcccaccgcg
     2161 actgcgtcga gtcccacctc gtgccggtgg ccgaggtgac gatgcacctg ccgatcgagg
     2221 tcgccgacta cgtcgacttc tacgccagcg agcaccacgc gacgaacgtc ggccggatgt
     2281 tccggcccga ctccgaaccg ctcaccccga actggaagca cctgccgatc ggctaccacg
     2341 gccgctccgg cacggtcgtc gtctcgggca ccgacatcgt gcgcccctgc ggccaacgaa
     2401 agccccccac cgagccggcg ccggtgttcg ggccgtcggc tcgcctggac atcgaggccg
     2461 agctcggctt cgtcgtcggc ggcgcgtcgc gcctcggcga acccgtgcct ctcgcggatg
     2521 ccggggagca cctgttcggt gtcgtcatcc tcaacgactg gtcggctcgg gacctccagg
     2581 cgtgggagta cgtgccgctc gggcccttcc tcggcaagtc gttcgcgacg tcgatctccc
     2641 cctgggtggt cacgatggag gccctgggcg cggctcacgt gcggctgccc gggcaggacc
     2701 ccgaaccgct gccctacctg cgcggtgagc cgaccgcgga cggcgccggc acggcatacg
     2761 ggctcgacat cagcctcgag gtggagtgga acggcaccgt cgtcgcgagg ccgcagttcc
     2821 gcgacatgta ctggtcgccg gcgcagatgc tcgcccacat gacggtcaac ggggcgagtg
     2881 tgcgcaccgg agacctgttc gggtcgggca cgatctctgg tgagcagaag gacacccgcg
     2941 gcagcttcct ggagctgtcg tggagcggga cggagccgat cacgctggcc gacgggtcac
     3001 agcggacctt cctcgaggac ggcgacaccg tgaccctgcg ggccgtcgcc ccggggccgg
     3061 acggtgcggt catcgggctc ggcgagtgca cggggacggt gctcgccgcc cgctgagacc
     3121 ggggccgacg gcggtgcccg ccgaggctca gcgcgccttg aggacgacca ccgtgtctgc
     3181 cagcttgtcg tgcagggtct gacgcttggg gtcccagagc agccacgcgg ggtcgaggat
     3241 gctgacgatc gagcccagca gccccacgac cggcaggaca ccgacggcgg cggtggcgag
     3301 cccgatgagc tgacgcttga tggcatccac cacggtgagc ttgcccggtg atccgacgcg
     3361 acggaccacc gtgcccagcg ccatcttgcc gggggtggca ccccgccaga cgaggaagac
     3421 cgtctcgtag agcaggccca cgaagaggct gacgatcgtg atcgggagac tggccgtcag
     3481 catctcggtg atgtaggcgt tctggtcggg catcgcgcca cccgcctctg ccgcggccac
     3541 ggtggcgtcg acgaagtcgg tgacggccgc gaacatgggc ccgagccacg ggatggccac
     3601 aaccagcgcc acgatgctcg tgatgaagct gtcgaggatg cgggccagca ggcgccgcca
     3661 ccactgggcg agcaccgcac cgtcaggaag cacgtcacgg cgctgcgcgt aggccgcgcc
     3721 gtaaccgccg taggccggcc cagcgccgta tccctgaccg cccccgtagc ccggtcccga
     3781 ccagccaccc tgcccgggac cctggggccc gccctgctgg gccgcacccg gctgggtcgc
     3841 gccggggtga acccacccgc cctgcaggcc tgcgccgtcc ggctgctgcg caccacggcc
     3901 gatcaccgag tcgtcgatcg tcggtgactt gcgcggcgtg gtgtgcgaac tccacaccac
     3961 gccgtcgaag tagcgcagct gggaggcgtc gtcggggtcg tcgtaccagc cgggtccgga
     4021 aggggtctcg ctcatggcct cagggtctca cgcgtgcgat gactcccgga cacggcacac
     4081 acccaaccgg tgcaccgcct cggtcagagg ttgccgcggc gctcctgctc gcgctcgatc
     4141 gactcgaaca gggccttgaa gttgcccttg ccgaagccga gcgagccgtg ccgctcgatg
     4201 atct
//
LOCUS       DAOSOC010000192         4188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_21302_length_4188_cov_7.830390, whole genome shotgun sequence.
ACCESSION   DAOSOC010000192 DAOSOC010000000
VERSION     DAOSOC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21302_length_4188_cov_7.830390"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..1824)
                     /locus_tag="PL014_11505"
     CDS             complement(325..1824)
                     /locus_tag="PL014_11505"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOT57761.1"
                     /translation="MSPDRLEVVVLVVSAVLLLGVAAVRVSTGAGLPSLLLYLGIGLA
                     IGEAGLGLRFDDAAVAQTVCTVALAVILAEGGLTTRLHVIRPVALHAGVLATLGVAIS
                     VAVTSTAAWLLLDVDVRTALLLGAVVSSTDAAAVFAVMRRLPLNRRTRALLEAESGLN
                     DPPVIILVTLVVSSAWEDLDGWAALFGFVQQMGLGVVAGVLVAFAGRFVLSRVALPAT
                     GLYPLATMTFAMLGFGVAGVVGGSPFVAVYLAGLLLGNADLPHRAATLGFAEGLAWLS
                     QIGLFVVLGLLASPSRLADALVPALVVGVALTLVARPLSVALCSIPFRMPWREQAFTS
                     WAGLRGAVPIVLATFPITAGVPNAQQIFDVTFLLVVVFTVIQGPTLPWAASRLAVVEP
                     EITAELEIEHAPFEDIGASMLTCDIPKGSLLHGVEISELRLPGDATVALILRDGILFA
                     PKPSTMLRRGDHLLLAVTDSERPGVERRLRAISRAGRLATWRGELGLLS"
     gene            complement(1927..2937)
                     /locus_tag="PL014_11510"
     CDS             complement(1927..2937)
                     /locus_tag="PL014_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HOT57762.1"
                     /translation="MITVTAARKNYGDFVALDGVGLEIPSGSLTALLGPSGSGKSTLL
                     RSIAGLEALDGGTVTIAGVDVTREPPQRRGIGFVFQHYAAFKHMTVRDNVAFGLTIRK
                     RPKAEVKRKVDELLEIVGLDGFQHRYPAQLSGGQRQRMALARALAVDPQVLLLDEPFG
                     ALDAKVRADLRQWLRRLHDEVHVTTVLVTHDQEEALDVADRIAVLNHGRIEQVGDPVS
                     LYERPANDFVMSFLGSVARLGGHLVRPHDIVLERDLAVAQRGDARNTGSPGVVTATIE
                     RVVRLGFEVRVDLRATTGERFAAQITRAEADRHQYVQGETVHARAVHPADATDADPAL
                     AI"
     gene            complement(2934..3743)
                     /gene="cysW"
                     /locus_tag="PL014_11515"
     CDS             complement(2934..3743)
                     /gene="cysW"
                     /locus_tag="PL014_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOT57763.1"
                     /translation="MIATRRSTLALRALALVYVLGLLAVPILVILYRTFEPGVGAFFD
                     SIRTPAAIAALNLSLLIVAIVVPLNVVFGIVTAIALARWRVPGRGVVQAVVDLPFAVS
                     PIVVGVSLIMLWGSSGWFGGLEASTGVKVIFGLPGMVIATIFVTLPFIVREVEPVLHE
                     IGTEQEQAASTLGASGWQTFWRITLPAIRWGLTYGVVLTVARALGEFGAVIMVSSGFP
                     GVSQTLTLLVHSRYIDDHNTYGAYAAATLLMGLAVATLLLMTLLDRKRSAR"
     gene            complement(3740..>4188)
                     /locus_tag="PL014_11520"
     CDS             complement(3740..>4188)
                     /locus_tag="PL014_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT57764.1"
                     /translation="PVGVQLNATRWGLVVALAFVTLPFVVRSVQPVLIELDREVEEAA
                     ASLGASNATTFRRVVLPTLAPAIISGAGLAFARAVGEYGSVVLIGGNIPRETQVVSQY
                     IQQQIEIDRPVNAAAVSVALLSISFLTLFVLRLWGGRAQRREERAS"
BASE COUNT          735 a         1433 c         1501 g          519 t
ORIGIN      
        1 tcggaaggtc ggcggttcga ccccgcccct ggccaccaca gcatcgcatc gcattacccc
       61 gagccgcagg tcaccactgg tgaacctgcg gcttcgtcgt tccaggcgcg actggtggtt
      121 ggggtggtga aggtactggt ggtcaggcgt cccgggcacc tacgctttcg ggcacaccga
      181 ccgccgaaga gagcacgatg cgctacctgc tgggagcagt tgccctcgca ccgttcgcgc
      241 tgctcctgct ggccatggtc acgggccgtg ccacggtcca accctgctgt gcaccagcgg
      301 cgcccgcgga cccgaaccca gatgtcagga cagcaggccc agctcaccgc gccaggtcgc
      361 gaggcggccg gcccggctga tcgcccgcag ccgccgctcg accccaggtc gctcggagtc
      421 ggtgaccgcc agcagcaggt ggtcgccgcg gcgcagcatc gtcgacggtt tcggtgcgaa
      481 gaggatgccg tcacgcagga tcagcgcgac cgtcgcgtcc ccgggcagcc tgagttcgga
      541 gatctcgacc ccgtgcagca gcgacccctt ggggatgtcg caggtgagca tgctggcccc
      601 gatgtcctcg aacggtgcgt gttcgatctc cagctcggcg gtgatctccg gttcgacgac
      661 ggccagccgg gatgccgccc agggcagggt cggcccctgg atgacggtga agacgacgac
      721 gagcaggaag gtgacgtcga agatctgctg cgcgttcggc acccctgccg tgatggggaa
      781 ggtggccagc acgatgggaa ccgcaccgcg caacccagcc cacgaggtga acgcctgctc
      841 gcgccacggc atccgaaacg ggatggaaca cagcgcaacc gacagggggc gcgccacgag
      901 ggtcagggcg accccgacga cgagcgctgg caccaacgca tcggccagcc ggctcggtga
      961 ggcgagcagc cccagcacga cgaacaggcc gatctgcgag agccaggcca ggccctcggc
     1021 gaagcccagg gtggccgccc ggtgcggcag gtcggcgttg ccgagcagca ggcccgcgag
     1081 gtagaccgcc acgaacgggc tcccaccgac caccccggcg acgccgaagc cgagcatcgc
     1141 gaacgtcatc gtcgccagcg ggtagaggcc ggtggccggt agggcgaccc gcgacagcac
     1201 gaaccgcccg gcgaacgcca cgaggactcc ggcgacgaca cccaggccca tctgctggac
     1261 gaatccgaac agggcagccc acccgtcgag gtcctcccag gccgaggaga cgacgagggt
     1321 gacgaggatg atgacgggtg ggtcgttgag ccccgactcg gcctcgagca gggcccgcgt
     1381 gcggcggttc agcggcagcc ggcgcatgac ggcgaacacc gcggccgcgt cggtggagga
     1441 cacgacggcc ccgagcagca gtgcagtgcg cacgtcgacg tcgagcagca gccacgcagc
     1501 cgtcgaggtc acggccacgc tgatcgcgac ccccagggtc gcgaggaccc ctgcgtgcag
     1561 ggccaccggg cggatgacgt gcaaccgggt cgtcaggccc ccctcggcga ggatgacggc
     1621 cagcgccacg gtgcacaccg tctgcgccac ggcggcgtcg tcgaaccgca ggcccagacc
     1681 cgcttcaccg atggccagcc cgatgccgag gtagagcagc aggctcggca ggccggcgcc
     1741 cgtggacacg cgaaccgccg ccacccccag cagcagcacg gccgagacga cgaggacgac
     1801 gacctcgaga cggtcaggcg acatgtgcgg gggcacggct gacggcatcc atggaacaag
     1861 cctgacccgg ctggatcgcg cgcaccatgg cggcaggacc ccatttacgc cgccgaggtt
     1921 gacgcctcag atcgcgagcg ccgggtcggc gtccgtggca tcggccgggt gaacggcccg
     1981 tgcgtgcacc gtctcacctt ggacgtactg gtgccggtcg gcctcggccc gggtgatctg
     2041 ggcggcgaat cgttcaccgg tggtcgccct caggtccacc ctgacctcga acccgaggcg
     2101 cacgacccgc tcgatcgtcg cggtcacgac accgggagac cccgtgttgc gtgcatcgcc
     2161 tcgctgcgcg acggcaaggt ctcgctcgag gacgatgtcg tgagggcgca cgaggtgtcc
     2221 gccgagccgc gccaccgacc cgaggaagga catgacgaag tcgttggcgg gccgctcgta
     2281 gagcgacacg gggtcaccca cctgctcgat acggccatgg ttgagcaccg cgatgcggtc
     2341 agccacgtct agggcctcct cctggtcgtg ggtgacgagc accgtggtga cgtggacctc
     2401 gtcgtgcagc cggcgcagcc actggcgcag gtcggcgcgg accttggcat cgagagcccc
     2461 gaacggctcg tcgagcagga gcacctgcgg gtcgaccgcg agggcccggg ccagggccat
     2521 ccgctgtcgt tgaccaccgg acagctgcgc ggggtagcgg tgctggaagc cgtcgagacc
     2581 gacgatctcc agcaactcgt cgaccttgcg cttcacctcg gccttgggcc gcttgcggat
     2641 ggtcaggccg aaggcgacgt tgtcccgcac ggtcatgtgc ttgaacgcgg cgtagtgctg
     2701 gaagacgaag ccgatgcccc ggcgctgcgg cggctctcga gtgacgtcga ctccggcgat
     2761 ggtgacggtg ccaccgtcga gagcctccaa cccggcgatg gatcgcagca gggtcgactt
     2821 accggacccg ctggggccga ggagcgccgt gagggagccc gaggggatct cgagcccgac
     2881 accgtccagg gccacgaagt ccccgtagtt cttgcgggct gcggtgacgg tgatcatcgg
     2941 gcgctcctct tgcgatccag aagtgtcatg agaaggagcg tcgcgacggc caggcccatc
     3001 aggagggtgg cagcggcgta ggccccgtag gtgttgtggt cgtcgatgta gcgcgagtgc
     3061 acgaggaggg tgagcgtctg ggagaccccg gggaaccctg aggacaccat gatcaccgcg
     3121 ccgaactcgc ccagggctcg ggccacggtg agcacgaccc cgtaggtgag cccccagcgg
     3181 atggctggca gggtgatccg ccagaacgtc tgccaccccg acgccccgag agtcgaggct
     3241 gcctgttcct gctccgtgcc gatctcgtgc aggaccggtt cgacctcacg cacgatgaag
     3301 ggcagggtca cgaagatggt cgcgatgacc atgccgggga ggccgaagat gaccttgacg
     3361 ccggtcgagg cctccagacc gccgaaccag cccgaggaac cccacaacat gatcagggag
     3421 acgccgacga cgatgggtga cacggcaaag ggcaggtcga ccacggcctg gaccacgccg
     3481 cggccgggaa cgcgccaccg ggccagggcg atggccgtga cgatcccgaa caccacgttg
     3541 aggggtacga cgatggccac gatgagcagg gacaggttga gagccgcgat cgcggcgggc
     3601 gtccggatgc tgtcgaagaa cgcacccacg cccggctcga acgtgcggta gaggatgacg
     3661 aggatcggca cggccagcag gccgaggacg tagaccagcg ccagggcgcg cagggccagg
     3721 gtcgagcgtc tggtcgcgat catgaggccc tctcctcccg gcgctgggcg cgcccacccc
     3781 acagccgaag cacgaacagg gtgaggaacg agatcgagag cagcgccacc gacacggccg
     3841 ccgcattgac cgggcggtcg atctcgatct gctgctggat gtactgcgag accacctgcg
     3901 tctcgcgggg gatgttgccg ccgatgagca ccaccgagcc gtactcgccg accgcgcggg
     3961 cgaaggccag cccggcgccg ctgatgatgg cgggagcaag ggtcggcagg accacccggc
     4021 gaaacgtcgt ggcgttggac gcacccaggg atgccgcggc ctcctccacc tcccggtcca
     4081 gttcgatcag gaccggctgg accgagcgga cgacgaacgg cagcgtcacg aaggccagcg
     4141 ccacgaccag gccccaccgg gtggcgttca gctgaacgcc gaccgggc
//
LOCUS       DAOSOC010000193         4109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_21829_length_4109_cov_8.517267, whole genome shotgun sequence.
ACCESSION   DAOSOC010000193 DAOSOC010000000
VERSION     DAOSOC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21829_length_4109_cov_8.517267"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(2..197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(380..1174)
                     /locus_tag="PL014_11525"
     CDS             complement(380..1174)
                     /locus_tag="PL014_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57765.1"
                     /translation="MSRHSMPGRRRAPGAVRRLPDGFSRLGATWAAKTVATVGVVGGA
                     VLAVSLPVSASPTTLALPAAASSDGQSRTAAFTASRSEARAAAPAAPAVEAPADAAPA
                     APETVGVAGVKAVAKPKPKPKPKPKPVAEKASSSSSSSSSSSARKVNVSPGAVSSKCG
                     SIGLDSNAARLCTVVQKTFGVSSIGGYRPNAGEHSTGEAIDFMISSRSQGDAIAAFVQ
                     ANVGAYDVKYVIWRQRYWAPGKSWRMMEDRGSITANHYDHVHVTVN"
     gene            complement(1343..2041)
                     /locus_tag="PL014_11530"
     CDS             complement(1343..2041)
                     /locus_tag="PL014_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT57766.1"
                     /translation="MTDLIDTTEMYLRTIFELEEEGITPLRARIAERLGHSGPTVSQT
                     VARMERDGLLAVAGDRHLELSEQGRTLATRVMRKHRIAERLLVDIIGLEWEYVHDEAC
                     RWEHVMSDRVERKILAMLPDHALSPYGNPIPGLEELGDFGERIDFRRGLSTLEEVAGA
                     EPATVVVRRIGEPVQVDSEALGLLTTVGLVPGQPARVRRDGVRVIAWRDGSQESTGVS
                     LPGDIASHVFVQVT"
     gene            complement(2078..>2468)
                     /locus_tag="PL014_11535"
     CDS             complement(2078..>2468)
                     /locus_tag="PL014_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT57767.1"
                     /translation="VMDRYGRVWVAVPVALLLGGGLLVLPLTASLVGVAVVTLVMGVG
                     NGIGSGIVMTLGADAAPTVGRAPFLGVWRLLSLVGTNGAAVVLAAVAAVASIAAASVA
                     VGALTLVGGAWLARWLPEYDPRRGRAT"
     assembly_gap    2469..2568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2569..3319
                     /locus_tag="PL014_11540"
     CDS             <2569..3319
                     /locus_tag="PL014_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57768.1"
                     /translation="PVDITIPQRFCGPPESGNGGWVSGTLATHVATTDAHPAVTVRLA
                     APPPLDRPLTLVVDTPVPDAVAPVPAAARLLDGDHLVASATASAPLAQEAQTPVTVAA
                     AHAAEERYEGLSDHPFPTCFSCGTARDPDDALCLRPGPLDDESGRYATSWIPREASVP
                     LVWAALDCPGGWSAGIAGRPMVLGTMTTEVIALPEPGVAHTVLAWHRGSQGRKHFSGS
                     ALVAPDGRVLARAEATWIAVDPTTIRPREIS"
     gene            3316..4080
                     /locus_tag="PL014_11545"
     CDS             3316..4080
                     /locus_tag="PL014_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT57769.1"
                     /translation="MTDTTRTAVVTGASSGIGAATARELVGQGFRVVCAARRADRLEA
                     LVAELGDTAVAVACDVTNDDDVARLADVAGDRVHVLVNNAGGALGLEPVAQGDVALWQ
                     QMYDTNVLGTLRVTKALLPALVAADGEGIIVNVGSVAGFTAYEGGGGYTVAKHGVHVM
                     SETLRLELVDQPVRITVVAPGMVQTEEFSLTRFGGDKERADAVYAGVPGPLVAQDVAD
                     AIVWMATRPAHVNVDLLVIKPRAQAAAHKVQRLANP"
BASE COUNT          604 a         1478 c         1387 g          540 t
ORIGIN      
        1 gtgagcctct cagtgcctac ggggtgagct gtcgggttcg ggctgaggtt gcccggccgg
       61 gtccgacccg aaggtcgcca ccggcttcac cccaaggagt cgcgatggtg ccggactccg
      121 aaagtggttc ccccgctcct gccagcggtg atgcgtggtg cctcacggtg tctgacagga
      181 ctcggcggtg actccgtgag caccttccac ggatgggaag gccatacgaa agtagcaccg
      241 cccggcaaaa gtcacaaaac gatcacgggc gagtcgcgtc acctcgccgg ggccgcgccc
      301 gtccctactg cgagaagccg ggcaacggcc gtggggacgg cgcgttgccc ggcttctcgc
      361 cggaaaggtc aggaagtcgt cagttgacgg tgacgtgcac gtggtcgtag tggttcgcgg
      421 tgatcgatcc gcgatcctcc atcatccgcc aggacttgcc gggcgcccag tagcgctgac
      481 gccagatgac gtacttgacg tcatacgcac cgacgttggc ctggacgaac gcagcaatgg
      541 catcgccctg gcttcgggac gagatcatga agtcgatcgc ctcaccggtg ctgtgctccc
      601 cggcgttggg tcggtagcca ccgatcgagg agaccccgaa ggtcttctgc accacggtgc
      661 acagccgggc cgcgttgctg tccagaccga tgctgccgca cttggaggac acggcccctg
      721 ggctgacgtt gaccttgcgg gccgacgacg aggacgatga agaggaggag gacgaggcct
      781 tctcggcgac cggcttgggc ttcggcttcg gcttcggctt gggcttggcg acggccttga
      841 caccggccac gccgacagtc tcgggcgctg ccggagcggc atccgcgggg gcctccacgg
      901 cgggggctgc gggcgcggcg gcgcgggcct cggagcgcga ggccgtgaac gcggccgtgc
      961 ggctctgacc gtcgctgctc gctgcggcgg gcagcgccaa ggtggtcggg ctcgccgaga
     1021 ccgggaggga caccgcgagg acggcaccgc ccacgactcc gaccgtcgcg acggtcttgg
     1081 cggcccaggt cgcgccgagg cgcgagaagc cgtcggggag ccgccggacg gcgccggggg
     1141 cgcggcggcg cccgggcatg ctgtggcgtg acacgaggtt ggaggacctt ccgagggggg
     1201 taagaattcc cccaacggta acggggacct aggggaaagt cacattctgg taacgagacg
     1261 acggtcacca ggtgtcacga acagccaccc ggcacgccgc tgacctgcac ggacgcgaac
     1321 gtcactgcgg caggagcgac tctcaggtca cctgcacgaa gacgtgggag gcgatgtccc
     1381 ccgggagcga gaccccggtc gactcctgcg acccgtcgcg ccacgcgatg accctcaccc
     1441 cgtcgcgccg cacccgggcg ggctgccccg gcaccagacc gacggtggtc agcagcccga
     1501 gcgcctcgga gtcgacctgc accggttcac cgatccggcg taccaccacg gtcgcgggct
     1561 cggcaccagc cacctcctcg agcgtggaca ggccgcgacg gaagtcgatc cgctcgccga
     1621 agtccccgag ctcttcaagc cccgggatcg ggttgccgta gggagagagc gcgtgatcag
     1681 gcagcatcgc gaggatcttg cgctcaaccc ggtcggacat gacgtgctcc caccgacagg
     1741 cctcgtcgtg gacgtactcc cactccaggc cgatgatgtc gacgagcagg cgctccgcaa
     1801 tgcggtgctt gcgcatgacg cgggtcgcga gcgtgcggcc ctgctcgctc agctcgaggt
     1861 gccggtcgcc ggccaccgcg agcagcccgt cacgctccat ccgtgccacc gtctgcgaga
     1921 ccgtggggcc ggagtgcccg aggcgttcgg cgatgcgggc acgcaggggt gtgatgccct
     1981 cttcctcgag ctcgaagatc gtgcggaggt acatctccgt ggtgtcgatg aggtcggtca
     2041 cacaggtaag cctaacgggc cgccagccca tcccccctta cgtcgcccga ccccgcctcg
     2101 ggtcgtactc gggcagccat cgcgcgagcc atgcgccgcc gaccagggtc agcgcaccga
     2161 cggccacgct ggcggccgcg atcgacgcca cggcagccac cgccgcgagg acgaccgcag
     2221 ccccgttcgt gcccacgagg gagagcaggc gccacacgcc gaggaagggt gcccgcccga
     2281 ccgtcggcgc ggcatccgcc cccagggtca tgacgatgcc ggagccgatc ccgttgccga
     2341 cccccatgac cagcgtgacc acggccacgc ccacgagcga cgccgtgagg ggcagcacga
     2401 gcagaccccc accgaggagc agcgcaaccg gcacggccac ccacacccgt ccgtaccggt
     2461 ccatgacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ccgtggacat
     2581 caccatcccc cagcgcttct gtgggccgcc tgaatccggc aacggcggct gggtcagcgg
     2641 caccctcgcc acccacgtcg cgaccaccga cgcgcacccc gccgtcacgg ttcggctggc
     2701 tgcgccccca ccgctggatc gtcccctgac gctcgtcgtc gacacccccg tcccggatgc
     2761 cgtcgcgccc gttccagccg cggcccgcct cctcgacggc gaccacctcg tcgcgagcgc
     2821 cacggcatcc gcacccttgg ctcaggaggc gcagaccccg gtcaccgtgg cggccgcgca
     2881 cgccgccgag gagcgctacg agggcctgag cgaccacccg ttcccgacgt gcttctcctg
     2941 cggcaccgcc cgcgaccccg acgacgcgct ctgcctgcgc ccggggccgc tcgatgacga
     3001 gagcgggcgc tacgccacct cgtggatccc gcgcgaggcg tcggtgccgc tggtctgggc
     3061 tgcgctcgac tgccccggcg ggtggtccgc cggcatcgcc gggcgcccga tggtgctcgg
     3121 cacgatgacc accgaggtca tcgcccttcc cgaacccggc gtcgcccaca cggttctcgc
     3181 gtggcaccgc gggtcccagg gtcgcaagca cttctccggc agcgccctcg tcgcacccga
     3241 cggacgggtg ctggcccgtg ccgaggccac gtggatcgcc gtcgacccca ccacgatccg
     3301 cccgagggag atctcatgac cgacaccacc cgcaccgccg tcgtcacggg agcgagcagc
     3361 ggcatcggcg ccgccaccgc ccgcgagctc gtcggtcagg gctttcgggt cgtgtgcgcg
     3421 gcccgacggg ccgaccggct cgaggctctc gtcgccgagc tcggtgacac ggccgtggcc
     3481 gtggcgtgcg acgtgaccaa cgacgacgac gtcgcccgcc tggccgacgt cgcgggtgac
     3541 cgggtgcacg tgctggtcaa caacgccggc ggggcgctcg gcctcgagcc ggtggcgcag
     3601 ggcgacgtcg cgctgtggca gcagatgtac gacaccaacg tcctcggcac cctgcgcgtc
     3661 accaaggccc tgctgcccgc actggtcgcc gccgacggtg agggcatcat cgtcaacgtc
     3721 ggctcggtgg cgggcttcac cgcctacgag ggcggcgggg gctacaccgt cgccaagcac
     3781 ggcgtgcacg tcatgagcga gaccctgcgg ctcgagctgg tcgaccagcc ggtgcggatc
     3841 acggtcgtcg cgccggggat ggtgcagacc gaggagttct cgctgacccg cttcggcggc
     3901 gacaaggaac gtgcggatgc cgtgtacgcc ggcgtccccg gacctctcgt cgcgcaggac
     3961 gtcgccgacg cgatcgtgtg gatggcgacg cgaccggcgc acgtcaacgt cgacctgctc
     4021 gtcatcaagc cgcgggccca ggcggccgcc cacaaggtcc agcgcctcgc caacccctga
     4081 cgctccccct atgcgacttc gtgcgactt
//
LOCUS       DAOSOC010000194         4100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_21901_length_4100_cov_10.686279, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000194 DAOSOC010000000
VERSION     DAOSOC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21901_length_4100_cov_10.686279"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>2188
                     /gene="scpA"
                     /locus_tag="PL014_11550"
     CDS             36..>2188
                     /gene="scpA"
                     /locus_tag="PL014_11550"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOT57770.1"
                     /translation="MSAVPDFTTVDLGAAEPTPAAAADDAQPSGEVWLTPEQVPVKPL
                     YTEADLDGLDFLDTVPGAPPYLRGPYPTMYTTQPWTIRQYAGFSTAEESNAFYRRNLA
                     AGQKGLSVAFDLATHRGYDSDHPRVTGDVGMAGVAIDSIYDMRTLFDRIPLDQMSVSM
                     TMNGAVIPILALFVVAAEEQGVAPEQLTGTIQNDILKEFMVRNTYIYPPEPSMRIISD
                     IFAFTSQKMPRFNSISISGYHMQEAGATQDLELAYTLADGVEYLRAGKAVGLDVDVFA
                     PRLSFFWAIGMNFFMEVAKMRAARLLWARLVKDQGATNPKSLSLRTHSQTSGWSLTAQ
                     DVFNNVVRTCVEAMASTQGHTQSLHTNALDEAIALPTDFSARIARNTQLVLQQESGTT
                     RVIDPWAGSAYVERLTHDLARRAWEHLEEVEAAGGMAKAIEAGIPKMRIEEAAARTQA
                     RIDSGQQPVIGVNRYPVDEDEPIEVLKVDNAAVRASQVAKLERLRAERDEDATRAALA
                     KMTDAARSGSDGTLATNLLALAIDAARAKATVGEMSAAMEEVFGRYTAQIRTIGGVYS
                     AEAGQDESVLRAQALVEEFEAEQGRRPRILVAKMGQDGHDRGQKVISTAFADLGFDVD
                     VGPLFQTPAEVARQAVEADVHVVGVSSLAAGHLTLVPALRHELDALGREDLMIVVGGV
                     IPAQDFEALRAAGADAIYPPGTVISTAAIDLIGDLR"
     assembly_gap    2189..2288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2289..2705
                     /locus_tag="PL014_11555"
     CDS             <2289..2705
                     /locus_tag="PL014_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOT57771.1"
                     /translation="DGDGEGPARVAARELAGAMRLMMPGAATRRPPVLTCSALTGAGL
                     DEVWEAVLEHRAHLESEGSLEARRAQQQQDWMWAMVDAHLQDLARQTPSVHAQREQIE
                     QAVRAGDLSAVDGSARILELVTSDLTDRGSPLMDGC"
     gene            complement(3038..3517)
                     /locus_tag="PL014_11560"
     CDS             complement(3038..3517)
                     /locus_tag="PL014_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57772.1"
                     /translation="MTAAAMGDTRKLERMLASPRADLARAAVQQWVLTHPEPKPNLDS
                     PTAKQTMLAAAGSYDYYCTGYNGSTIGWNGKKFRACHGYLNSYISGSHVGRYLPDVYP
                     TGAKISAACKKAYAGATITLMGALTAGVPTGGWGWVAGTAAVGWAVQDVWFSCGRRY"
     gene            complement(3771..>4100)
                     /locus_tag="PL014_11565"
     CDS             complement(3771..>4100)
                     /locus_tag="PL014_11565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT57773.1"
                     /translation="AAFALAHGSLDPWILLELGSLAFFGCYLAWRTGGLEAVIVIHVI
                     NNILILVTGALFGGLAESYVDGTSEGSPVSAVLNLVVTAATTAVLVWLARRRGIAPAG
                     WLTPARG"
BASE COUNT          671 a         1377 c         1352 g          600 t
ORIGIN      
        1 ccacgtgatg ccggcaccac cacggaaggt tcccgatgag cgccgtcccc gacttcacca
       61 ccgtcgacct cggcgcggct gagccgaccc cggccgccgc agccgacgat gcccagccct
      121 cgggggaggt gtggctgacc ccggagcagg ttccggtgaa gccgctctac accgaggccg
      181 acctcgacgg gctcgacttc ctcgacaccg tgccgggcgc gccgccctac ctgcgcggcc
      241 cgtacccgac gatgtacacg acgcagccgt ggacgatccg tcagtacgcc ggcttctcca
      301 ccgcggagga gtccaacgcc ttctaccggc gcaacctcgc ggccggccag aaggggctgt
      361 cggtcgcctt cgacctggcg acccaccgcg gctacgactc cgaccacccg cgggtgacgg
      421 gtgacgtggg catggccggc gtggcgatcg actcgatcta cgacatgcgc acgttgttcg
      481 accgcatccc gctggaccag atgtcggtgt cgatgaccat gaacggggcg gtcatcccga
      541 tcctcgcgct gttcgtcgtc gccgctgagg agcagggggt cgctcccgag cagctcaccg
      601 ggaccatcca gaacgacatc ctcaaggagt tcatggtccg caacacctac atctacccgc
      661 ccgagccgtc gatgcggatc atcagcgaca tcttcgcgtt cacgagccag aagatgccgc
      721 ggttcaactc gatctccatc tccgggtacc acatgcagga ggccggggcc acgcaggacc
      781 tcgagctggc gtacacgctc gcggacggcg tggagtacct gcgcgccggc aaggccgtcg
      841 gcctggacgt cgacgtgttc gccccgaggc tgtcgttctt ctgggccatc ggcatgaact
      901 tcttcatgga ggtggccaag atgcgggccg cccgcctgct gtgggcgcga ctggtcaagg
      961 accagggcgc caccaacccg aagtcactca gcctgcgcac gcacagccag acctcgggct
     1021 ggtccctgac ggctcaggac gtgttcaaca acgtcgtgcg cacgtgtgtc gaggcgatgg
     1081 cctcgaccca gggccacacc cagagcctgc acaccaacgc cctcgacgag gccatcgcgt
     1141 tgccgaccga cttctcggcg cgcatcgccc gcaacaccca gctggtgctc cagcaggagt
     1201 cgggcacgac ccgggtcatc gacccgtggg cgggctcggc ctacgtcgag cgcctcaccc
     1261 acgacctcgc gcgccgcgcg tgggagcacc ttgaggaggt cgaggccgcc ggtggcatgg
     1321 cgaaggcgat cgaggccggc atcccgaaga tgcgcatcga ggaggccgca gcccgcaccc
     1381 aggcccgcat cgactccggc cagcagccgg tcatcggcgt caaccgctac ccggtcgacg
     1441 aggacgagcc gatcgaggtg ctcaaggtcg acaacgccgc ggtgcgggcg agccaggtcg
     1501 ccaagctcga gcggctgcgg gccgagcgcg acgaggacgc cacccgtgca gcgctcgcga
     1561 agatgacgga tgccgctcgc tcgggttccg atggcacgct cgctacgaat ctgctggccc
     1621 tggcgatcga tgctgctcgc gccaaggcga ccgtggggga gatgtcggcg gcgatggagg
     1681 aggtcttcgg ccggtacacc gcgcagatcc gtacgatcgg tggcgtgtac agcgctgagg
     1741 cagggcagga cgagagcgtg ctgcgtgccc aggcgctcgt cgaggagttc gaggcagagc
     1801 agggccgccg cccgcgcatc ctcgtcgcga agatgggcca ggacgggcac gaccgcggcc
     1861 agaaggtcat ctcgaccgcg ttcgccgacc tcgggttcga cgtcgacgtg ggcccgttgt
     1921 tccagacgcc ggccgaggtc gcgcgccagg ccgtcgaggc cgacgtgcac gtcgtcgggg
     1981 tcagttcgct cgcggccggg cacctcacgc tcgtgccggc gctgcgccac gagctcgacg
     2041 ccctgggccg tgaggacctg atgatcgtcg tcggcggcgt catccccgcg caggacttcg
     2101 aggcgctgcg ggctgccggt gcggacgcga tctacccgcc gggcacggtc atcagcacgg
     2161 cggcgatcga cctcatcggc gacctgcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnga cggcgacggc gaggggccgg cgcgggtggc cgcgcgcgag cttgctgggg
     2341 cgatgcggtt gatgatgcca ggtgctgcca ctcgacgacc ccctgtactg acctgctcgg
     2401 ccctgaccgg tgccgggctc gacgaggtgt gggaggcggt gctcgagcac cgggcccacc
     2461 tggagtccga aggatcgctg gaggcgcggc gcgcgcagca gcagcaggat tggatgtggg
     2521 cgatggtcga tgcccacctg caggacctgg cgcgacagac gccgtctgtt cacgcgcagc
     2581 gtgagcagat cgaacaggcc gtgcgcgccg gcgacctgag cgcggtcgat ggcagcgccc
     2641 gaatcctcga gctcgtcacc tccgacctca ccgaccgggg cagcccgctg atggacggat
     2701 gttgatcgat tctctgtctg cctacgtcgg gggctggtgc ctcccgaaag agttgccgtg
     2761 gatcccgatg atgccaatgc accgcggaga ccccacggca agtcgccccg actatcgctg
     2821 gtgagggctc tcgtcggttg gctgtcgacg tcggaggctg gtgaccaccc atccgaggag
     2881 cgcaacttgc cacagcacga ggaggaggac cgcaatcggc gactgctcct ctgcggcaac
     2941 ggtccgccac acggcccaca caagactcgc ctcaagaagg ccaaaggcaa tccacatccg
     3001 gggggtcatc acagttttcc tttccactgc tgagtactca gtaccgacgg ccacaactga
     3061 accagacgtc ctgaaccgcc catccgacgg ccgccgtgcc agcgacccat ccccagccgc
     3121 ccgtcggcac cccagccgtg agtgcgccca tgagcgtgat tgtcgcgcca gcgtaggcct
     3181 tcttgcacgc ggccgaaatc ttggctcctg tgggatagac gtctggcagg taacggccta
     3241 catgtgaacc gctaatgtag gagttcaggt agccatggca cgcgcggaac ttcttgccgt
     3301 tccacccgat cgttgacccg ttgtaccccg tgcagtagta gtcgtacgac cctgcggccg
     3361 ccaacatggt ctgcttcgcg gttggactgt cgaggtttgg cttcggctcc gggtgggtga
     3421 ggacccactg ctgcacggcg gcgcgagcaa gatccgccct cggggaggcc agcattctct
     3481 caagcttcct ggtatcgccc atcgccgcag ccgtcatggc agcaagagcc tcggggtcca
     3541 gatcgctgac cgtggctgta ggcgactgct ctgctatcgg gcgggggggg ttgcctggct
     3601 gggagcggcg aacaccagca acatcgcaag caacaccaag atcgaactga ctaccttcgt
     3661 tctcatcgac tcatccccat cgtggttgag atatccacgc cagttggcgc gaacggcgtt
     3721 gagtcttgca ccagcccacc caccgcgcat cctgagcgac aggttcgcag tcagccgcgg
     3781 gctggagtga gccagccagc gggggcgata ccgcggcgcc gggccagcca caccagcacg
     3841 gccgtggtcg cggctgtgac gacgaggttg agcacggccg agaccggtga gccctcgctc
     3901 gttccgtcga cgtaggactc cgccaggcca ccgaacaggg ccccggtgac caggatcagg
     3961 atgttgttga tgacgtggat gacgatgacg gcttcgagcc cgccggtgcg ccacgcgagg
     4021 tagcagccga agaaggccag cgacccgagc tcgagcagga tccacgggtc gagcgacccg
     4081 tgcgccaggg cgaaggccgc
//
LOCUS       DAOSOC010000195         4089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_21975_length_4089_cov_10.176252, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000195 DAOSOC010000000
VERSION     DAOSOC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21975_length_4089_cov_10.176252"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1598)
                     /gene="ffh"
                     /locus_tag="PL014_11570"
     CDS             complement(3..1598)
                     /gene="ffh"
                     /locus_tag="PL014_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT57774.1"
                     /translation="MFTSLSDRLTETFKNLRSKGRLTESDVNKTIRDIRLALLDADVA
                     LPVVKEFTHAVRERALGAEVSEALNPAQQVVKIVNEELVGILGGQTRTIAFAKQPPTV
                     IMLAGLQGAGKTTLAGKLSKWLKDQGHTPLLVAADLQRPNAVTQLEVVGERAGVPVFA
                     PERGNVFGHDVVSETGEGTRSHGDPVAVSRAGVEHARRAQHDVVIVDTAGRLAIDANL
                     MQQAADIRAAIQPDEVLFVIDAMIGQAAVETARAFQDGVDLTGVVLTKLDGDARGGAA
                     LSVASVTGRPIMFASTGEGVKDFEVFHPDRMASRILDMGDILTLIEQAEKAFDRTHAA
                     EMERKFLAAEDFTFDDFLQQMAAIKKMGSLKSILGMMPGMGQMKAQIDAFDEREFDRV
                     EAMVRSMTPFERNHPKQINGSRRARIAKGSGTSVSEVNQLLERFTDAQKMMKQLARGG
                     GIPGMPGMPGGGSGPGKRGRQQVPQRKKSKSGNPAKRAAEQRAAAERPASGGGAFGAP
                     GAGDPATIDPASLPKGFEKFLGQ"
     gene            complement(1760..>2308)
                     /locus_tag="PL014_11575"
     CDS             complement(1760..>2308)
                     /locus_tag="PL014_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT57775.1"
                     /translation="LDITDLDRVGLFADTAGLLAAESHTVRTAILRTVDGIAVNEWHV
                     ESPSGAVPDAARIERGLARLAQGDRGPLTTLDRRRAHHPRPSGRSSIGTPGQARALIV
                     PGASSDATVLEVRAQDRPGLLHEIGAAFASAGISVRSAHIATYAGQTLDTFYLTGADG
                     LPLPPARVAQAVSLIIDTCDGI"
     assembly_gap    2309..2408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2409..3628)
                     /locus_tag="PL014_11580"
     CDS             complement(<2409..3628)
                     /locus_tag="PL014_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOT57776.1"
                     /translation="MTTPSSELSSRRLDLAGTRDFAVAGAGPARRAALAAFNRRWLAG
                     IWASATGGSRIEGIALAAVGSIGRGDAGPLSDYDLVLLHSERSLPAKELTALADRIWY
                     PIWDAGVRLDHSVRTVTQCRQVAASDLTAAVGLLDLAPLAGDEQLVVAARSTVAHDWR
                     GNARKRLPLLVEAVASRHQRQGDLAQSIEPELKEAHGGLRDMTVLSALAEAWLADRPH
                     GGVDAAQATLLDVRDAVHVVTGRGRDRLGREDHDAVAALLGHQDSDDLLTAVSHAGRV
                     IAYALDGTLRRAGQSQRARTLRVGPRRPQMTPLGHGLFSSDGEVVLGSARLAEADAGM
                     PLRAGVVAARAGLPIAPATLDNLASSSPLPEAPWPPWMRDLLGDLLAAGPGLVGVWEG
                     LDQVGIIDRWIPEWA"
     gene            complement(3631..3969)
                     /locus_tag="PL014_11585"
     CDS             complement(3631..3969)
                     /locus_tag="PL014_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT57777.1"
                     /translation="MKLVTAIIKPHQLDEVKEALEAFGIQGMTISEAHGYGRQRGHSE
                     VYRGAEYTVDFVPKSRIEVLVDDMDSSSVVDVILKAAQTGRIGDGKIWVVPVEDVARV
                     RTGERGVEAI"
BASE COUNT          550 a         1353 c         1493 g          593 t
ORIGIN      
        1 ggtcactgcc ccaggaactt ctcgaagccc ttgggcaggc ttgccgggtc gatggtcgcc
       61 gggtcgcccg cgccgggagc gccgaaggca ccaccgccgg atgccgggcg ctcggccgcc
      121 gctcgctgct cggctgcgcg cttggccggg tttccggact tggacttctt gcgctggggc
      181 acctgctgac ggccacgctt gcccgggccg gagcctccgc ccggcatccc cggcatgccc
      241 gggatgcccc cgccgcgagc gagctgcttc atcatcttct gggcgtcggt gaagcgctcg
      301 agcagctggt tgacctcgga aacgctggtg cccgacccct tggcgatgcg ggcccggcgc
      361 gagccgttga tctgcttggg gtggttgcgc tcgaacgggg tcatcgagcg caccatggcc
      421 tcgacccggt cgaactcgcg ctcgtcgaag gcgtcgatct gggccttcat ctggcccatg
      481 cccggcatca tgccgaggat cgacttcagc gagcccatct tcttgatggc cgccatctgc
      541 tgaaggaagt cgtcgaaggt gaagtcctcg gcagcgagga acttgcgctc catctccgcg
      601 gcgtgggtgc ggtcgaacgc cttctcggcc tgctcgatga gggtgaggat gtcacccatg
      661 tcgaggatgc gagaggccat ccggtcgggg tggaagacct cgaagtcctt cacgccctcg
      721 ccggtgctcg cgaacatgat cgggcggccg gtgaccgagg caaccgacag ggcagcacca
      781 ccgcgggcgt cgccgtcgag ctttgtcagc acgacaccgg tgaggtcgac cccgtcctgg
      841 aaggcgcggg cggtctcgac cgcagcctga ccgatcatcg cgtcgatgac gaacaggacc
      901 tcgtcgggct ggatcgccgc acggatgtcg gcggcctgct gcatgaggtt cgcgtcgatg
      961 gcgaggcgac ctgcggtgtc gacgatgacg acgtcgtgct gggcccggcg cgcgtgctcg
     1021 acaccggcgc gcgagacggc caccgggtcg ccgtggctgc gggtgccctc acccgtctcg
     1081 gagacgacgt cgtggccgaa gacgttgccg cgctcaggcg cgaagaccgg cacgccagcg
     1141 cgctcaccga cgacctcgag ctgggtgacg gcgttcgggc gctggaggtc ggcggccacg
     1201 agcagagggg tgtggccctg gtccttgagc cacttcgaga gcttgccggc caaggtcgtc
     1261 ttgccggcac cctggaggcc ggcgagcatg atgacggtcg gcggctgctt ggcgaacgcg
     1321 atcgtgcgcg tctgtccccc gaggatgccg acgagctcct cgttgacgat cttcacgacc
     1381 tgctgcgcgg ggttcagcgc ctcgctgacc tcggccccca gcgcgcgctc acgcacggcg
     1441 tgggtgaact ccttgaccac cggcagcgcg acatcggcgt cgagcagggc gagacggatg
     1501 tcgcggatcg tcttgttgac gtccgactcc gtgagacgac ccttgctgcg gaggttcttg
     1561 aaggtctcgg tcaggcggtc ggagaggctg gtgaacaccg gtcaaggcta acggctgatg
     1621 ccgacgggcc ccgaccgcca acaccctcac cgcgaacgtg ggtgaaaatc gtcggctgga
     1681 cgacgatctt cacccacgtt cgcaggaggg ccggagggtt cgcaggaggg tcggaggttt
     1741 cgcaggaggg tcgcgtggct cagatgccgt cacacgtgtc gatgatgagg ctgaccgcct
     1801 gcgcgacccg agcgggcggc aggggcaacc cgtcggcccc ggtcaggtag aaggtgtcga
     1861 gggtctggcc ggcataggtc gcgatgtggg ccgagcgcac cgagatgccg gccgacgcga
     1921 aggcggcacc gatctcgtgg agcagtccgg gccggtcctg ggcacgcacc tcgagcacgg
     1981 tggcgtcgct gctcgcgccg gggacgatca gcgctcgcgc ctgacccggc gtgccgatcg
     2041 acgaccgccc tgacggcctc gggtggtgtg cccgacgccg gtccaaggtc gtgaggggtc
     2101 cacgatcccc ctgcgcgagg cgagccaacc cgcgctcgat gcgcgcggca tccggcaccg
     2161 ctccactcgg cgactcgacg tgccactcgt tgacggcgat cccgtcgacg gtgcgcagga
     2221 tggccgtgcg cacggtgtgg ctctcagccg cgagcaggcc cgcggtgtcg gcgaagagcc
     2281 cgacccgatc gaggtcggtg atgtcgagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnngc ccactccggg atccaccggt cgatgatgcc gacctggtcc agcccttccc
     2461 agaccccgac gaggccgggt ccggcggcga gcaggtcacc gaggaggtca cgcatccacg
     2521 gcggccacgg ggcctcgggc agcggcgagc tggaagcgag gttgtccagc gtcgccgggg
     2581 cgatcggcaa cccggcccgg gccgcgacca caccggcccg cagcggcatc cccgcgtcgg
     2641 cctcggcgag ccgggcggag ccgaggacga cctcgccgtc gctggagaac agcccgtggc
     2701 cgagcggcgt catctgcggg cggcgcggcc cgacgcgcag ggtcctcgcg cgctgcgact
     2761 ggccggcccg gcgcagcgtg ccgtcgaggg cgtaggcgat gactcgtccg gcgtgcgaga
     2821 ccgccgtcaa gaggtcgtcg gagtcctggt ggcccaggag cgcggcgacg gcgtcgtggt
     2881 cctcgcgtcc gagccggtca cggccccggc cggtgacgac gtgcacggcg tcgcggacgt
     2941 cgagcagggt ggcctgggcg gcatcgaccc cgccgtgggg tcggtcggcg agccaggcct
     3001 cggccagggc ggacagcacg gtcatgtccc gcaggccgcc gtgggcctcc ttgagctcgg
     3061 gctcgatgga ctgggcgagg tcaccctgtc gctggtggcg cgaggccacg gcctcgacca
     3121 gcaggggcag ccgcttgcgg gcgttgccgc gccagtcgtg ggccacggtc gagcgggccg
     3181 cgacgacgag ctgctcgtca cccgccaacg gagcgaggtc gagcagcccc acggcggcgg
     3241 tgaggtcgga tgccgcgacc tggcggcact gcgtcaccgt gcggacgctg tggtcaagac
     3301 gcaccccggc atcccagatc gggtaccaga tgcggtcggc gagcgccgtc agctccttgg
     3361 cgggcagcga gcgctcggag tgcaggagga cgaggtcgta gtcgctgagg gggccggcgt
     3421 caccgcggcc gatgctgccg acggccgcca gggcgattcc ctcgatccgg ctcccacccg
     3481 tggcgctcgc ccagatcccg gcgagccacc gacggttgaa ggctgcgagg gcggcccggc
     3541 gtgccgggcc ggcacccgcg acggcgaagt cgcgggtgcc ggccaggtcg agccgcctgg
     3601 agctgagctc gctcgagggg gttgtcatgc tcagatcgcc tcgacgccgc gctctccggt
     3661 gcggacccgg gcgacgtcct cgaccgggac gacccagatc ttgccgtcac cgatgcggcc
     3721 ggtctgggcg gccttgagga tgacgtcgac cacggaggag gagtccatgt cgtcgacgag
     3781 cacctcgatc cggctcttgg gtacgaagtc gacggtgtac tcggcaccgc ggtagacctc
     3841 gctgtggcca cgctggcgcc cgtagccgtg ggcctcgctg atggtcatgc cctggatgcc
     3901 gaaggcctcg agggcctcct tcacctcgtc gagctggtgg ggcttgatga tggcagtgac
     3961 gagcttcatg cctgggctcc ttcgaggttc ttggccgggg cgccagtggg ggcggcgctg
     4021 gggccaccgc tcatccgacc accgcgagag ttgatgtcgt aggccgactc agcgtgctcg
     4081 gagccgtcg
//
LOCUS       DAOSOC010000196         4086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_21997_length_4086_cov_10.677499, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000196 DAOSOC010000000
VERSION     DAOSOC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21997_length_4086_cov_10.677499"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PL014_11590"
     CDS             complement(<1..1052)
                     /locus_tag="PL014_11590"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HOT57778.1"
                     /translation="MNTYWCEHAALPDGIRRSVRIEVAEGRIAAVTPGEPARPGDVVV
                     HGVTLPGLANGHSHAFHRALRGRTHDDGGNFWTWRTAMYAVTHRLDPQTYQALATAVF
                     AEMVLAGFTVVGEFHYLHHAAGGRRYDDPNAMGVALLDAARAAGIRITLLDTVYLEGG
                     LTADGHLPLDEVQQRFSDGSVAAWAQRVDLLADDATSRIGTAVHSVRAVPAHALAAVG
                     EFAALGERPLHVHLSEQPGENAACQGFHGCSPTELLAREGLLTPATTAVHATHLSDRD
                     IELLADTRTTACFCPTTERDLADGIGPARGLHDAGAPLSIGSDQHAVIDPFEEIRGVE
                     MHERLHTLERGRFSPL"
     gene            complement(1049..2347)
                     /locus_tag="PL014_11595"
     CDS             complement(1049..2347)
                     /locus_tag="PL014_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HOT57779.1"
                     /translation="MASPGGAVGPPSPAVNGFRRLWQDLHDIGRSRDTGGYNRFAWTR
                     EDHDLREWFTAQCQQRDLDVTTDRMGNQWAWWGDPDAAVAAGDPGVVIGSHLDSVPDG
                     GAFDGPLGVVGALAAIDHLRATGFTPSRPIGAVNFVDEEGARFGVACAGSRVITGVLG
                     ADRARGLTDRDGTSMAEAMRAAGRDPGTLGRDDETLARIGTFVELHVEQGRRLVDLGH
                     AVGVGSDIWPHGRWRVDLPGEANHAGTTRLEDREDAMLGLAAMIIAARRAATEHGCVA
                     TVGKVNVVPGGVNAIASGATGWLDARGADADAVRRVVADVGAVVADFDGLVTQESWTP
                     TTMFDPALAQQMAARLGGVPVIGTGAGHDAGILANDGIRTAMLFVRNPTGVSHSPDEW
                     ASADDCVAGVLALADVVSDLAGADAARGGERDEQAEGPAA"
     gene            2472..2945
                     /locus_tag="PL014_11600"
     CDS             2472..2945
                     /locus_tag="PL014_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOT57780.1"
                     /translation="MTTTGEGRAQDALTASGIVFSITRHGRVGSLAEAAAARGVEPRD
                     IVKTLVVRRGEGDFLFVLVPGDREISWPKLRAHLGVNRVSMPDAATALEVTGYERGTI
                     TPFGSTTAWPVIADVSMTGRRVSLGAGAHGVAATVEADAALVALGADVADITDPL"
     gene            complement(3071..>4086)
                     /gene="hutU"
                     /locus_tag="PL014_11605"
     CDS             complement(3071..>4086)
                     /gene="hutU"
                     /locus_tag="PL014_11605"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOT57781.1"
                     /translation="DDAVEKVLRAKAERRAWSVGVVGNAAEIFPELLRRGVPIDIVTD
                     QTSAHDPLSYLPEGVALEEWAELAEKKPEEFTDRARASMAKQVKAMVEFQDAGAEVFD
                     YGNSIRDEARQGGYERAFEFPGFVPAYIRPLFCEGKGPFRWAALSGDPKDIAVTDKAV
                     LDLFPDNDRLHKWIRGAQERIAFQGLPARICWLGQGERDKAGLAFNDLVRTGAVQAPI
                     VIGRDHLDTGSVASPYRETESMLDGSDAIADWPLLNALVNTASGASWVSIHHGGGVGI
                     GRSLHAGQVSLADGTDLAAQKLERVLTNDPAMGVIRHVDAGYDIAERTAAEQGVRIPM
                     RES"
BASE COUNT          537 a         1419 c         1556 g          574 t
ORIGIN      
        1 tcgagcgggc tgaaccgacc acgctcgagg gtgtggaggc gttcgtgcat ctcgaccccg
       61 cggatctcct cgaaggggtc gatgaccgcg tgctggtccg acccgatcga cagcggcgcc
      121 ccggcatcgt gcaggccgcg cgccgggccg atgccgtcgg cgaggtcgcg ctcggtggtc
      181 gggcagaagc acgcggtcgt gcgggtgtcg gcgagcagct cgatgtcgcg atcgctgagg
      241 tgggtggcgt gcacggcggt cgtcgcaggg gtcagcagcc cctcgcgagc gagcagctcg
      301 gtcggcgagc acccgtggaa accctggcag gcggcgttct cgcccggctg ctccgacagg
      361 tgcacgtgca gcggtcgctc cccgagcgcg gcgaactcgc cgacggccgc cagggcgtgg
      421 gccggcacgg cgcgcaccga gtgcaccgcg gtgccgatgc ggctggtcgc gtcgtcggcg
      481 agcaggtcga cgcgctgcgc ccaggcagcg acgctgccgt cgctgaagcg ttgctgcacc
      541 tcgtcgagtg gcaggtggcc gtcggcggtg agcccaccct cgaggtagac cgtgtcgagc
      601 aacgtgatcc ggatgccggc cgcgcgcgcc gcgtcgagca gcgccacccc catcgcgttc
      661 gggtcgtcgt accggcggcc accggccgcg tggtggaggt agtggaactc cccgacgacg
      721 gtgaacccgg cgaggaccat ctcggcgaag accgccgtgg ccagggcctg gtaggtctgc
      781 gggtcgaggc ggtgggtcac ggcgtacatc gccgtgcgcc acgtccagaa gttgccgccg
      841 tcgtcgtggg tgcgtccgcg cagggcccgg tggaaggcgt ggctgtggcc gttcgcgagg
      901 ccgggcaggg tgacgccgtg cacgacgacg tcgcccgggc gggcgggctc accgggtgtg
      961 acggcggcga tcctgccctc ggccacctcg atgcgcaccg agcgccggat gccgtcgggc
     1021 agggcggcgt gttcgcacca gtacgtgttc acgcggcggg cccttcagcc tgctcatcac
     1081 gctcgccacc gcgcgcggca tccgcccccg cgaggtcgct gacgacgtcg gcgagtgcca
     1141 gcaccccggc gacgcagtcg tcggccgagg cccactcgtc gggcgagtgc gacaccccgg
     1201 tcgggttgcg cacgaacaac atcgccgtgc ggatgccgtc gttcgcgagg attccggcgt
     1261 cgtggcccgc acccgtcccg atcaccggga cgccacccag ccttgccgcc atctgctgcg
     1321 cgagagccgg gtcgaacatc gtcgtcgggg tccaggactc ctgggtcacg agcccgtcga
     1381 agtcggcgac gacggccccg acgtccgcga cgacccgccg cacggcatcc gcatcggcgc
     1441 cgcgcgcatc gagccagccc gtggcgccgc tggcgatcgc gttgacaccg cccggcacga
     1501 cgttgacctt gcccaccgtg gcgacgcagc cgtgctcggt ggcggcccgg cgggcggcga
     1561 tgatcatggc ggcgaggccg agcatggcgt cctcgcggtc ctcgaggcgg gtggttccgg
     1621 cgtggttggc ctcgccgggg aggtcgacgc gccaccgacc gtggggccag atgtcgctgc
     1681 ccacaccgac ggcgtggccg aggtcgacga ggcggcgacc ctgctcgacg tgcagctcga
     1741 cgaaggtgcc gatccgggcg agcgtctcgt cgtcgcgccc gagcgtgccg gggtcgcgcc
     1801 cggcagctcg catcgcctcg gccatcgagg tgccgtcacg gtcggtcagg ccacgggctc
     1861 tgtccgcacc gaggacgccg gtgatgacgc gcgagcccgc gcaggcgacc ccgaagcggg
     1921 cgccctcctc gtcgacgaag ttgaccgcgc cgatggggcg gctcggggtg aagccggtcg
     1981 cccgcaggtg gtcgatggcc gcgagggcgc cgacgacgcc gagcgggccg tcgaacgcgc
     2041 cgccgtctgg gaccgagtcc aggtgcgacc cgatgaccac cccggggtca cccgcggcga
     2101 ccgcggcgtc ggggtcgccc caccaggccc actggttgcc catccggtcg gtggtgacgt
     2161 cgagatcgcg ctgctggcac tgggcggtga accactcgcg caggtcgtgg tcctccctgg
     2221 tccaggcgaa tcggttgtag ccgccggtgt cgcgggaacg accgatgtcg tgcaggtcct
     2281 gccacagtcg tcggaagccg ttcacggccg ggctcggggg gccgaccgca cccccggggc
     2341 tcgccatcgt gaccatgggt ccattggacc aactccggcg ccgtcgcggg ggcgcaagag
     2401 cggtggactt gtctcgggtg ccagacgagt ggcccgtccc ccgtcgccgg gtcgggccgt
     2461 actctgggcc gatgacgacg acaggcgagg gccgagcgca agatgcgctg acggcatccg
     2521 ggatcgtttt ctccatcacg cgacacgggc gcgtcgggtc gttggccgag gcggccgccg
     2581 cgcgcggggt cgaaccgcgc gacatcgtca agacgctcgt ggtgcgacgc ggggagggcg
     2641 acttcctgtt cgtgctcgtg ccgggcgacc gggagatctc gtggcccaag ctgcgggcgc
     2701 acctcggagt gaaccgcgtg tcgatgccgg atgccgcgac ggccctcgag gtcacggggt
     2761 acgagcgcgg cacgatcact ccgttcggct cgacgacggc ctggccggtc atcgccgacg
     2821 tgagcatgac cgggcgcagg gtgtcgctcg gggcgggggc acacggtgtc gcggcgacgg
     2881 ttgaggcgga tgccgcgctg gtcgccctgg gtgccgacgt cgccgacatc accgaccccc
     2941 tctagaaccg acttcgtgcg acttgcgtgc ggaaatgagc cacttctcgg gctcggaccg
     3001 caccgaagtc gcacgaagtc ggggtgtggt cggcgggtgt gaggggtggg gcccgcctga
     3061 acccgacggg tcaggactcg cgcatcggga tgcggacacc ctgctcggcg gcggtgcgct
     3121 cggcgatgtc gtacccggcg tcgacgtgcc ggatgacgcc catggccggg tcgttggtca
     3181 gcacgcgctc gagcttctgc gcggcgaggt cggtgccgtc ggcgagggag acctggccgg
     3241 cgtgcagcga acggccgatg ccgaccccac caccgtggtg gatcgacacc cacgacgcgc
     3301 cggatgccgt gttgacgagc gcgttgagca gcggccagtc ggcgatcgcg tcggacccgt
     3361 cgagcatcga ctcggtctcg cggtaggggc tggccaccga gccggtgtcg aggtggtcgc
     3421 ggccgatgac gatcggggcc tggaccgcac cggtgcgcac gaggtcgttg aacgccaacc
     3481 cggccttgtc gcgctcaccc tggccgagcc agcagatccg cgccggcagg ccctggaagg
     3541 cgatgcgctc ctgcgcgccg cggatccact tgtgcaggcg gtcgttgtcg gggaacaggt
     3601 cgagcacggc cttgtcggtg acggcgatgt ccttggggtc acccgagagg gcggcccagc
     3661 ggaacgggcc cttgccctcg cagaacagcg gccggatgta ggccggcacg aagccgggga
     3721 actcgaacgc ccgctcgtag ccgccctgac gggcctcgtc acggatcgag ttgccgtagt
     3781 cgaagacctc ggcgccggcg tcctggaact cgaccatcgc cttgacctgc ttggccatcg
     3841 aggcccgggc gcgatcggtg aactcctcgg gcttcttctc ggcgagctcg gcccactcct
     3901 ccagcgccac accctcgggc aggtaggaca gcgggtcgtg ggcgctggtc tggtcggtga
     3961 cgatgtcgat cggcacgccg cggcgcagca gctcggggaa gatctcggca gcgttgccga
     4021 cgaccccgac cgaccaggcg cgacgctcgg ccttggcgcg cagcaccttc tcgacggcgt
     4081 cgtcga
//
LOCUS       DAOSOC010000197         4078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_22044_length_4078_cov_5.819041, whole genome shotgun sequence.
ACCESSION   DAOSOC010000197 DAOSOC010000000
VERSION     DAOSOC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22044_length_4078_cov_5.819041"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PL014_11610"
     CDS             complement(<1..584)
                     /locus_tag="PL014_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57782.1"
                     /translation="MRADAVSLAYDERIVVDGVTLDVPTGKITVVVGPNGCGKSTLLR
                     GLGRLLKPRGGSVLLDGEVITELPTREVARRLGLLPQQPIVPEGVSVLELVERGRHPH
                     HGLFRSWGRADEEAVASALTRTDLVELASVPVDSLSGGQRQRVWLAMVLAQDTPVLLL
                     DEPTSFLDIAHQLDVLDLVRSLRDQSGTTVVMVL"
     gene            complement(746..1882)
                     /locus_tag="PL014_11615"
     CDS             complement(746..1882)
                     /locus_tag="PL014_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT57783.1"
                     /translation="MATSTTDAVPSTTDAVPAPTATITASGTTASDAVASDAVARHLA
                     AARRGPRRRRALVVGVLLLLLVALVLVRALLGDYRVSFVDAIAIIGGEQIPGASFVLM
                     ESTLPRAVMAVLAGAAFGAAGSAFQVLLRNPLASPDVLGITLGASAGAVIAVVTFGVR
                     GTPVTLAALAGAFGAAALILSQAGGRGGATGRMVLVGVALAAGLSAVVHWVLVRASTY
                     QAQDALIWLSGSLNSVTWAEIARLLALDSVLIPLLLVLASALPVLGLGDDLAAGLGVR
                     VGRLRVGVTAIVVALVASATAVVGPIAFVGFLAGPIARRLVPGRPAIGIGALVGAVVV
                     VAADHVAAYAIPGTALPVGVVTGLLGAPVLLWMLRSRSTSPEGR"
     gene            complement(1882..2940)
                     /locus_tag="PL014_11620"
     CDS             complement(1882..2940)
                     /locus_tag="PL014_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT57784.1"
                     /translation="MTSTLTPPRVRKWSPWQPLPHSRQGKPVVIVVAAGLALIAVTSL
                     TSVLVGVQSLAPGDVLDALRGMPAGQETAAIVASRVDRTVIGLVVGAAIALSGVVLQG
                     LTRNPIAEPGILGLNAGAALAVVIGIRFLGLSSVTGYVVAALVGTIAVAVLVQSLTLM
                     APRASAPLSMALAGAAVMALAQSLIGAVIVTDRGALDSFRFWQVGSVAGRDATQVLDI
                     APFLLVGLVLALTSGPTLNAVALGDELARGLGQNVMLHRGLAAAGAVLLAACATALAG
                     PIAFVGLVVPHLVRAVVGVEHRRVLLVSLLLGPVFVVLADIVGRLIAQPGEVQAGIVC
                     AVLGAPVLVAVVRRVKGL"
     gene            complement(2937..3992)
                     /locus_tag="PL014_11625"
     CDS             complement(2937..3992)
                     /locus_tag="PL014_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT57785.1"
                     /translation="MPSRRHLLAAAPALALIALAGCSTGPSDTAASSSATSSTAVDAA
                     AYPVTITHAHGSTTIEQTPTRVATIGWADADVVAALGVVPVGAPKITWGGNADGSTDW
                     FDAKADELGVKDGDIARYDDSAGIPFDDIAATRPDLILGLSSGVSKEDYDKLSKIAPV
                     VPYTVAPWGTPWQDQLEAIGQALGRPTQAAALEASTTKTIADAVAANPQLKGRSAAWA
                     WFSPTDLSTIGLYTTADLRPQMLREFGLVDSPTVVELSKADPSAFSVNLSAEKASTLD
                     ADLVVFYAAEGTQTDDIMGNALLSQIPALRKKQFVASADNAIALSMSLPSPLSVRTAV
                     DDFLPKIATALSGTPTS"
BASE COUNT          656 a         1398 c         1541 g          483 t
ORIGIN      
        1 tgcagcacca tgacgaccgt ggtgccggac tggtcacgta gcgagcgcac gaggtcgagc
       61 acgtcgagct ggtgcgcgat gtcgaggaag gacgtcggtt cgtcgagcag cagcaccggc
      121 gtgtcctggg ccagcaccat ggccagccac acccgttgtc gctgaccgcc cgacaggctg
      181 tcgaccggca ctgacgcgag ctcgacgagg tcggtgcggg tcagtgccga cgcgacggcc
      241 tcctcgtcgg cgcggcccca gctacggaac aggccgtggt gcggatgccg cccgcgctcg
      301 acgagctcga gcacgctgac accctcgggc acgatcggct gctgcggcag caaccccaga
      361 cgccgagcca cctcacgcgt cggcagttcg gtgatcacct caccgtcgag cagcaccgac
      421 ccaccgcgtg gcttcagcag ccggccgagc ccgcgcagca acgtcgactt cccgcacccg
      481 ttgggcccga cgacgacggt gatcttgccc gtgggcacgt cgagggtgac cccatcgacg
      541 acgatccgct cgtcgtaagc caggctcacg gcatccgccc gcagtcgggg cgaaggctcc
      601 tgcgacctct gcgacgactc cgaccgctca gcctcctgcg aatgtgggtg aagaccgtcg
      661 gctggacgac gattttcacc cacgttccag cgaggggcag gggtcgaggg agaggtcgag
      721 gtgggggtgg tggtctctga cacggtcatc ggccctccgg ggacgtggat cgggagcgga
      781 gcatccagag caggacgggg gcgccgagca ggccggtgac gaccccgacg ggcagggcgg
      841 tgccggggat ggcgtaggcc gcgacgtggt cggcagccac gacgacgacg gctccgacga
      901 gggcgccgat gccgatggcg gggcggccgg gcacgaggcg gcgggcgatc ggcccggcga
      961 ggaacccgac gaaggcgatc ggcccgacga cggccgtcgc ggaggccacg agcgccacga
     1021 cgatcgccgt cacccccacg cgcagccgcc cgacgcgcac cccgagaccg gcggcgaggt
     1081 catctcccag cccgagcacg ggcagcgccg aggcgagcac gagcagcagc gggatgagca
     1141 cggaatccag ggccagcagg cgcgcgatct cggcccaggt gaccgagttc aggctgccgc
     1201 tcagccagat cagcgcatcc tgcgcctggt aggtgctcgc gcgcacgagc acccagtgca
     1261 cgaccgccga cagcccggcc gcgagcgcca ccccgacgag caccatccgg ccggtcgccc
     1321 caccgcgacc tccggcctgg ctgaggatga gcgccgccgc accgaacgcg ccggccagcg
     1381 ccgcgagcgt gaccggtgtg ccgcgcaccc cgaacgtcac gaccgcgatg acggccccgg
     1441 cgctggcccc gagggtgatg cccaggacgt cggggctcgc gagcgggttg cgcagcagca
     1501 cctggaacgc cgacccggcc gccccgaacg ccgcccccgc gagcacggcc atgaccgccc
     1561 gcggcagggt cgactccatg agcacgaacg acgccccggg gatctgctcg ccgccgatga
     1621 tcgcgatcgc gtcgacgaac gagacccggt agtcgccgag cagcgcccgc acgagcacga
     1681 gggcgacgag cagcaggagc aggacaccga cgacgagggc gcggcggcgg cgcgggcccc
     1741 gccgggcggc ggcgaggtgc cgggcgacgg cgtcggatgc caccgcatcg gatgccgtgg
     1801 tgccggatgc cgtgatggtc gccgtgggtg ccgggacggc atccgtggtt gacgggacgg
     1861 catccgtggt tgacgtcgcc atcacagccc cttgacccgg cggacgaccg ccacgaggac
     1921 gggtgccccg agcaccgcgc agacgattcc ggcctgtacc tcacccggtt gggcgatgag
     1981 gcggccgacg atgtcagcaa gcacgacgaa caccgggccg aggaggagcg agacgagcag
     2041 cacccgccgg tgctcgaccc cgacgacggc gcgcacgagg tgcggcacca cgagcccgac
     2101 gaacgcgatg ggcccggcca gcgccgtggc acatgccgcc aacagcaccg caccagcggc
     2161 ggccagccct cggtgcagca tgacgttctg cccgagcccg cgggccagct cgtcaccgag
     2221 ggcgacggcg ttgagcgtcg gaccggacgt gagggccagc acgaggccga cgaggaggaa
     2281 gggcgcgatg tcgagcacct gggtggcgtc acgaccggcg accgagccca cctgccagaa
     2341 ccggaagctg tccagggcac cccggtcggt gacgatcacg gcaccgatga ggctctgcgc
     2401 gagggccatg acggctgccc cggcgagtgc catcgacagc ggggcactcg cgcgcggcgc
     2461 catgagggtg agtgactgca cgagcacggc gacggcgatg gtgccgacga gcgccgcgac
     2521 gacgtacccg gtcacgctcg acagcccgag gaagcggatg ccgatgacga cggccagggc
     2581 agccccggcg ttcagcccga ggatgccggg ttcggcgatg gggttgcggg tcaacccctg
     2641 cagcaccacc ccgctgagag cgatggctgc cccgaccacg agcccgatga ccgtgcggtc
     2701 gacgcgggag gcgacgatcg ccgcggtctc ctggcccgcc ggcatccccc gcagcgcgtc
     2761 gagaacgtcc ccaggcgcaa gggactggac accgacgagc accgacgtca gcgaggtgac
     2821 cgcgatcagg gcgaggccgg ccgcgacgac gatgacgacg ggctttccct gcctcgagtg
     2881 cggaagtggt tgccagggcg accacttccg cactcgaggc ggggtgaggg tggaggtcac
     2941 gaggtgggcg tgccgctcag tgccgtggcg atcttgggca ggaagtcgtc gacggccgtg
     3001 cgcaccgaca gcgggctcgg cagcgacatg gacagggcga tcgcgttgtc ggcgctggcg
     3061 acgaactgct tcttgcgcag cgccgggatc tgcgagagca gggcgttgcc catgatgtcg
     3121 tcggtctggg tgccctcggc ggcgtagaag acgacgaggt cggcgtcgag cgtcgaggcc
     3181 ttctcggccg acaggttcac cgagaacgcc gacgggtcgg ccttgctcag ctcgacgacg
     3241 gtcggggagt cgacgaggcc gaactcacgc agcatctgcg gtcgcaggtc ggcggtggtg
     3301 tagaggccga tcgtcgacag gtcggtcggg ctgaaccacg cccacgccgc actcctgccc
     3361 ttgagctgcg ggttggcggc cacggcatcc gcgatggtct tcgtcgtgga ggcctcgagc
     3421 gcggcggcct gcgtgggccg gccgagcgcc tggccgatgg cctcgagctg gtcctgccag
     3481 ggcgtccccc acggcgcgac cgtgtagggc acgacagggg cgatcttgct cagcttgtcg
     3541 tagtcctcct tggagacacc cgaggacagg ccgaggatga ggtcgggcct ggtcgccgcg
     3601 atgtcgtcga aggggatgcc ggcggagtcg tcgtagcggg cgatgtcgcc gtccttgacg
     3661 ccgagttcgt cggccttggc gtcgaaccag tcggtggagc cgtcggcgtt gccaccccag
     3721 gtgatcttgg gggcgccgac ggggacgacc ccaagcgccg cgacgacgtc ggcgtcggcc
     3781 cagccgatgg tggcgacccg ggtgggggtc tgctcgatcg tcgtcgaccc gtgggcgtgg
     3841 gtgatcgtga cggggtaggc cgcggcgtcg acggcggtgc tgctcgtggc cgaggacgag
     3901 gccgcggtgt cactgggccc ggtcgagcac ccggcgaggg cgatgagggc cagcgcgggg
     3961 gcggcggcga gcaggtgacg gcgagacggc attcgggtga cctccggagc agcagacgta
     4021 ccaaggtaag gcaacccttg gttaggtaag cctcacctta atcacaccct gctacgtc
//
LOCUS       DAOSOC010000198         4031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_22424_length_4031_cov_9.747988, whole genome shotgun sequence.
ACCESSION   DAOSOC010000198 DAOSOC010000000
VERSION     DAOSOC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22424_length_4031_cov_9.747988"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..543)
                     /locus_tag="PL014_11630"
     CDS             complement(94..543)
                     /locus_tag="PL014_11630"
                     /inference="COORDINATES: protein motif:HMM:NF040041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HOT57786.1"
                     /translation="MRANNSHHLAAAARERAERTRAKALRALQDLEDAGHPVTYEAVA
                     SKAGVSRSWLYSQPGMRARIDQLRTRDLTSPTTRRLPQRQRASEDSLLRRLEATTERM
                     RQLEQDNRQLRHALAQALGAARTRRVTGEPSRRDTPEKRATRITGPC"
     gene            complement(533..2416)
                     /locus_tag="PL014_11635"
     CDS             complement(533..2416)
                     /locus_tag="PL014_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT57787.1"
                     /translation="MWAEAAPATDPAGQLSCTLPFCSLWIEHERNPFCKAHQTRWRMQ
                     GCPDVEAFIQRCLLAGRDRIDYGGLPPLLKLELQYAVQCRVDQARITLPAPVVNWTIR
                     QARDAHVSSLLDLPAAQWAERAGPKNNSYPAFLAFARDVVDMLHEGIGWEVEYPRDIW
                     RLDRLPGLTPNAANPNPRNRIRFDRIAQPWLRPLVKRWSRLRLSSGLTIGTVATDVIA
                     LTRFSTFLAQALPEVDTLAGLDRAVLERYLAWLATSGLGHGAREDAVTGVGMFFQAVR
                     QHDWDPSLAATAAFFTGDLPHRPARVSRRLAEHVMAQVEAPANLNRWPTPEGQLVTLI
                     LIQCGLRATDACALGFDCLVHDGAGAPYLRYFNHKMRREAAVPIDPDLEVQVRGQQQR
                     VAQRWPGQHPHLFPQLTGNVGGHRALSYHSYRGMLNTWLALCDIRDEHGEPVHLTPHQ
                     WRHTFACRLINRDVPQEVIRVLLDHESTQMTAHYARITDQTVRRRWEQATKVNIRGER
                     IDIDPDGPLAQAAWAKTRYAMATQSLPHGYCGLPVQKSCPHANACLTCPVFLTGPQFL
                     PELREQQRRTLTLIDKAEADGHTRMAQMNTEVLTNLNTMIGQLEQIDEPIGEQRAGGD
                     NAS"
     gene            complement(2794..3912)
                     /locus_tag="PL014_11640"
     CDS             complement(2794..3912)
                     /locus_tag="PL014_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT57788.1"
                     /translation="MRVQRVLIEGVRVESWTVLGEDGVPVEPVERFLGYLSAIERSPN
                     TVKAYAHDLKDWFAFLGRHGLDWRLVTLEDVAGWVAWLRLPPAAREGRVLVLPSVEHH
                     CTASSVNRKLAALASFCEFHARHGVPLAGLLVTTGPPGRRRASTSSFKPFLHHVTKSA
                     PTRGRAITLRSSAARPRVLTVAEAQAILDGCKHRRDRLLFALLLDTGVRIGEALGLRH
                     EDLDIAQPRVAVIARDNDNRARAKGGRSRFIPASAQLIRLYADYLGEEYGALDSDYVF
                     VNLWGRPHGHPLTYPAVYDLVGRLRRRTGIGFSPHWFRHTYATWLLRRGAGMESVKAL
                     LGHASITTTIDTYGHLTVEDARATLEAAGWFTGKEVRL"
BASE COUNT          598 a         1354 c         1420 g          659 t
ORIGIN      
        1 tcccttatct ttagtccgcc tccggggagg gtgactgacc tgcacgtttg tgtggtggac
       61 ggtgttgtcg acgcgagttc gggtgggctt ggttcagcag ggtccggtga ttctggtggc
      121 gcgtttctcg ggcgtgtcgc ggcgtgaggg ttcgccggtg acgcggcgtg ttcgggcggc
      181 gccgagggcc tgggcgaggg catggcggag ctggcggttg tcctgttcga gctgacgcat
      241 ccgctcggtg gtggcttcca ggcgacgcag cagtgagtcc tcgctggctc gctgcctctg
      301 cggcagcctc cttgtggtgg gcgatgtgag gtcgcgggtt cgtagctggt cgatcctggc
      361 ccgcatgccg ggttgggagt acagccagga gcgggacacg cccgccttgc tcgcgacggc
      421 ctcataggtg accggatggc cggcgtcctc gaggtcctgt agggcgcgca gcgccttggc
      481 gcgggtgcgt tcggcccgct ctcgtgccgc ggcggccagg tggtgggagt tgttagctcg
      541 cattgtcgcc gcctgctcgt tgctcgccga tcggctcgtc gatctgctcg agctgtccga
      601 tcatggtgtt caggttggtc agcacctcag tgttcatctg ggccattcgg gtgtgcccgt
      661 cggcttcggc cttgtcgatc agggtcaggg tccggcgttg ctgctcgcgc agctcgggaa
      721 ggaactgcgg tccggtcagg aagacggggc aggtcaggca ggcgttggcg tgagggcagc
      781 tcttctgcac tggcagcccg cagtagccgt gcgggaggga ctgggtggcc atcgcgtagc
      841 gggtcttggc ccaggcggct tgggccagcg gcccgtcggg gtcgatgtcg atccgctcac
      901 cgcggatgtt gaccttggtg gcctgttccc agcgtcggcg cacggtttgg tcggtgatcc
      961 gggcgtaatg cgctgtcatc tgcgtggatt cgtgatcgag caggacccgg atgacctctt
     1021 gggggacgtc ccggttgatc aaccggcagg cgaaggtgtg gcgccactgg tgcggggtga
     1081 ggtggaccgg ctcgccgtgc tcgtcgcgga tgtcgcatag agcgagccaa gtgttcagca
     1141 tcccgcggta gctgtggtag ctcagcgcgc ggtgcccgcc gacgttgccc gtgagctgcg
     1201 ggaacaggtg cgggtgctgc ccgggccagc gctgggcgac gcgctgctgc tgtcctcgga
     1261 cctgcacctc gaggtcgggg tcgatgggca cggcggcttc acggcgcatc ttgtggttga
     1321 agtagcgcag gtaaggcgcg ccagcaccgt cgtggaccag gcagtcgaag cccagcgcgc
     1381 acgcgtcagt ggcgcgtagg ccgcactgga tcaggatgag ggtgaccagc tgcccttcgg
     1441 gggttggcca ccggttcagg ttcgcggggg cctcgacctg agccatgacg tgctcggcca
     1501 ggcgccggct gaccctggct gggcgatggg gcaggtcccc ggtgaagaac gccgcggtgg
     1561 ccgccaggct ggggtcccag tcgtgctggc ggacggcctg gaagaacatg cccacgccgg
     1621 tgacggcgtc ttccctggcg ccgtgaccga gcccggaggt ggccagccag gccaggtagc
     1681 gttccagtac cgctcggtcc agcccggcga gggtgtccac ctcgggcagg gcctgcgcca
     1741 ggaaggtact aaagcgcgtc agggcgatga cgtcggtggc cacggtgccg atcgtcaacc
     1801 cggagctgag ccggagccgt gaccagcgtt tgaccagtgg gcgcagccag ggctgggcga
     1861 tccggtcgaa acgaatccgg ttccgcgggt tcgggttagc cgcgttcggg gtcaggccgg
     1921 gcagccgatc gagccgccag atgtcccggg ggtactccac ctcccagccg atgccctcgt
     1981 ggagcatgtc caccacgtcg cgggcgaacg ccaggaacgc cgggtagctg ttgttcttcg
     2041 ggccagcccg ctcagcccac tgcgcagccg gcaggtccag cagcgaggac acgtgcgcgt
     2101 cccgtgcctg ccggatcgtc cagttcacca ccggtgccgg cagcgtgatc ctggcctggt
     2161 cgacccggca ctggaccgcg tactgcagct ccagcttgag cagcggcggc agcccgccgt
     2221 agtcgatgcg gtccctcccc gcgagcaggc accgctgaat gaacgcctcg acatctgggc
     2281 acccctgcat ccgccaccgg gtctggtgcg ccttacagaa cgggttccgc tcatgctcga
     2341 tccacagcga gcagaacggc agagtgcaac tcagttgacc cgctggatcc gtcgctggcg
     2401 cggcctcggc ccacactgtc ggatcggggt gcccgctggc ggtgaaggcg tggcggtggc
     2461 gcatgcacaa ccccaggccg ctgcttccgt agcggcagcc gagcacggtg catccggtca
     2521 ggtctcgtcg cccgttgagc accgggccgg gatcagccag gaaggcagcc atgtccgggc
     2581 ggcctcggtc ggcccatctc ctctggtggg tcgcgcacaa ccggttccca ctcggcgagc
     2641 ggtcgcaacc agccaccgaa cagggcccgc ggcccaacac cggatgatca gggtcaggga
     2701 cgtacacctc gacgcggaac tcggcgcgga cctcaccgat cagtttctcc aacagtcccg
     2761 gccacgaggt cgtcccgagg tcggtgcggg cgctcacagg cgcacctctt tgccggtgaa
     2821 ccagcccgcg gcctccaacg tcgcccgggc gtcctcgacg gtcaggtgac cgtaggtgtc
     2881 gatcgtggtc gtgatcgagg catggcccag caatgccttc acggactcca tcccggcccc
     2941 tcgacgcagc agccacgtcg cgtaggtgtg tcggaaccaa tgcgggctga acccgatacc
     3001 ggtgcggcgg cgcagccgac cgaccaggtc atagaccgcc gggtaggtca gcgggtgccc
     3061 gtgcgggcgg ccccacaagt tgacgaagac gtagtccgag tccagcgccc cgtactcctc
     3121 acccaggtag tcggcgtaca gcctgatcag ctgcgcactg gccgggatga accgggaccg
     3181 gccacccttg gctcgggccc ggttgtcgtt gtcccgggcg atgaccgcca cccgtggctg
     3241 ggcgatgtcg aggtcttcgt gccgcaaccc cagcgcctca ccgatccgca ccccggtgtc
     3301 cagcagcagc gcgaagagca accggtcccg ccggtgctta cacccatcca ggatcgcctg
     3361 cgcctcggcc acggtcagca cccgcggccg cgctgcagag gacctcaacg tgatcgcacg
     3421 cccccgcgtc ggggcgctct tggtcacgtg gtgcaagaac ggtttgaacg aggacgtcga
     3481 cgcccgtcga cgccccggcg ggcccgtggt gaccagcaac cctgccagcg gcaccccgtg
     3541 ccgggcatgg aactcgcaga acgaggccaa cgccgcgagc ttgcggttca cgctcgacgc
     3601 cgtgcagtga tgctccaccg acggcagaac cagcacccga ccctcccgcg cagccggcgg
     3661 cagccgcagc cacgcgaccc acccggcgac gtcctcgagc gtgaccaacc gccagtccag
     3721 gccgtgccgc cccaagaagg cgaaccagtc cttcaggtcg tgcgcgtacg ccttgaccgt
     3781 gttcggcgat cgctcgatcg cggacaagta ccccaggaac cgctcgacag gctcgaccgg
     3841 gacaccgtcc tcaccgagca ccgtccagga ctcgacccga acaccctcga tgagcacacg
     3901 ttgaactcgc atgaactgcc tcccgccgtc gtgatggaca gcgcaaggta accgcgtcca
     3961 cgacgcaccc cgcaggacag acaaccctcg tcaactacgt catggacacg cgacaagccg
     4021 ctccagataa c
//
LOCUS       DAOSOC010000199         4016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_22543_length_4016_cov_8.190608, whole genome shotgun sequence.
ACCESSION   DAOSOC010000199 DAOSOC010000000
VERSION     DAOSOC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22543_length_4016_cov_8.190608"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PL014_11645"
     CDS             <1..455
                     /locus_tag="PL014_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOT57789.1"
                     /translation="ALGLDPEDVVEMAWVDNGPGWAGILLRSPDAVLAADPDAGRCRG
                     VKVGLVAVYPQGSRADGVAAEVRAFYSDGREFAEDPVTGSLNAGLAQWLVPAGHLPPQ
                     YVAAQGTVIHREGRVHVDVVDGEVWVGGDTRTIFTGHLPEPKRPETDQ"
     gene            complement(527..1015)
                     /gene="greA"
                     /locus_tag="PL014_11650"
     CDS             complement(527..1015)
                     /gene="greA"
                     /locus_tag="PL014_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT57790.1"
                     /translation="MTETANQASFLTQGAFDRLQAELDELSGPGRTEIAKKIEAAREE
                     GDLKENGGYHAAKEEQGKMEARIRQLTQLLETAIIGEKPADDGVVEPGMVVTVEMFGD
                     KETFLLGSREITDESLNVFSEKSPIGAAVNGRKVGDTASYTAPNGKQIEVKILETTPY
                     DV"
     assembly_gap    1311..1410
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1475..2413
                     /gene="mca"
                     /locus_tag="PL014_11655"
     CDS             1475..2413
                     /gene="mca"
                     /locus_tag="PL014_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HOT57791.1"
                     /translation="MSDRLRLMCVHAHPDDESSKGAATMAKYVDEGHDVLVVSCTGGE
                     RGDVLNPRLKDDAHILRDLAQVRRDEMAAAQAILGVQHTWLGFVDSGLPEGDPLPPLP
                     EGCFALEPLSVTTEALVREIRRFRPHVVTTYDENGGYPHPDHIMCHDVSMAAVVAAGD
                     PEAFPHAGPAWQPLKVYYNQTFSKARVEAFHQALTALGEESPYAEWLENWTRPDRAVT
                     TRVECSAWFDRRDAALVAHATQVDPDGAFFRVPREVQVQVWPTEDYEAALSFVPIAPD
                     EDDLFAGLPADVRAADALATSGNLTIVHDTRTERDS"
     gene            2410..2721
                     /locus_tag="PL014_11660"
     CDS             2410..2721
                     /locus_tag="PL014_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57792.1"
                     /translation="MTDAPSAAVAPGFFAFVAFFIMAMVLWFLMRNMNARMRRMSYRQ
                     QAAHRGRDEAQAGPGDVSDSRRNDDDRADDRSPADDSSSKDTGSSDSGGSDGGGGGGS
                     D"
     gene            complement(2742..3476)
                     /locus_tag="PL014_11665"
     CDS             complement(2742..3476)
                     /locus_tag="PL014_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOT57793.1"
                     /translation="MTPLDPPATPDQSRREALEQRVEELEQRVEAAFDHVKPKLRGWL
                     HAGMVPVALIAGIVLVVLATTTTGKVSAAIFGGTAVLLFGTSAVYHRGTWSPRMGAFL
                     RRFDHSNIFLIIAGTYTPFALLLPPDQARHMLTIVWAGAIIGVLFRVFWSHAPRWLYV
                     PAYVMLGWVAVFNFGPLLEHAGAAVMTWIVIGGALYSAGAIVYGTKKPDISPRWFGFH
                     EVFHTLTILAFGSHFVAASLALSAPA"
     gene            3688..>4016
                     /locus_tag="PL014_11670"
     CDS             3688..>4016
                     /locus_tag="PL014_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOT57794.1"
                     /translation="MALSDVLYAAYARRLLRELEPSSVPRHVGVMLDGNRRWARARGA
                     GASEGHQAGADNIENLLSWCDEVGVDVVTLWLLSTDNLNRPEDELRPLLGIIEAAVDT
                     LAATHRWR"
BASE COUNT          599 a         1338 c         1391 g          588 t
ORIGIN      
        1 ccgccctggg cctggacccc gaggacgtcg tggagatggc gtgggtcgac aacggacccg
       61 ggtgggccgg catcctgctt cgttctcccg acgccgtcct ggccgcggac cccgacgccg
      121 gtcggtgtcg cggggtcaag gtcgggctgg tggcggtcta tccgcagggc tcacgtgccg
      181 acggcgtcgc cgccgaggtc agggcgttct actccgatgg ccgagagttc gccgaggacc
      241 cggtgaccgg gagcctcaac gccgggctcg cccagtggct ggtgccggcg gggcacctgc
      301 cgccgcagta cgtcgccgcg cagggaaccg tcatccatcg cgaggggcgg gtgcacgtcg
      361 acgtcgtgga cggtgaggtg tgggtcggcg gcgacacccg cacgatcttc accgggcacc
      421 tccccgaacc gaaacgtccc gagaccgacc aataggtcga tctcgggacg ctgtggggtc
      481 ggctctgccg tacctggtgt gacctattgg tcgatctcgg tacggatcag acgtcgtacg
      541 gcgtggtctc gaggatcttg acctcgatct gcttgccgtt cggtgcggtg taggaggcgg
      601 tgtcgcccac cttgcgcccg ttgaccgcgg caccgatcgg ggacttctcg gagaacacgt
      661 tcagcgactc gtcggtgatc tcgcgcgagc cgagcaggaa ggtctccttg tcgccgaaca
      721 tctcgacggt gacgaccatg cccggctcga cgacgccgtc gtccgccggc ttctcgccga
      781 tgatggccgt ctcgagcagc tgggtcagct gtcggatgcg ggcctccatc ttgccctgct
      841 cctccttggc ggcgtggtag ccaccgttct ccttgaggtc gccctcctcg cgggccgctt
      901 cgatcttctt ggcgatctcg gtacgtcccg ggccggagag ctcgtcgagc tctgcctgga
      961 ggcggtcgaa ggccccctgg gtcaggaagc tcgcctggtt ggcggtctcg gtcacgtcac
     1021 tcactcctgg tcatggtgtg gcaccccgcc cggtgtgaca ggggcgcccg atagacaacg
     1081 gcaacgggcc cgggtcgcgg tgctgtgcgg cacgaggtcg gacccagacc cgatgtgaaa
     1141 cgtcaatggt agcaaccggg tgggtgatcg gcgggtcagg gggtcgaggc gacccgtatg
     1201 catgagtcca cgacgccggt caccgcgcga acgctggtgc gaacgcgcac ctcacgccgc
     1261 agcgacgtgg gaccggcggc gggcacggca tcctcgacgg tgccgacgcg nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggatgagagg atgtcgaggt ccattctcgg
     1441 gtacgcccga acacccccgt acgaagggtc ccacgtgtct gaccggctcc gtctcatgtg
     1501 cgtccatgcg cacccggacg acgagtccag caagggtgcc gccaccatgg ccaagtacgt
     1561 cgacgagggc catgacgtcc tcgtcgtgtc gtgcaccggt ggcgagcgcg gcgacgtcct
     1621 caacccgcga ctgaaggacg acgcccacat ccttcgcgac ctcgcgcagg tgcgccgtga
     1681 cgagatggct gcggcccagg cgatcctcgg ggtgcagcac acctggctcg gcttcgtcga
     1741 ctccggtctg cccgagggcg acccgctgcc gccgttgccg gagggctgtt tcgccctcga
     1801 gccgctcagt gtcaccaccg aggcgctggt gcgtgagatc cgccggttcc gcccgcacgt
     1861 cgtcacgacc tacgacgaga acggtggcta tccgcacccg gaccacatca tgtgccacga
     1921 cgtgtcgatg gccgcggtcg tcgccgctgg cgacccggag gccttcccgc acgccggccc
     1981 cgcgtggcag ccgttgaagg tgtactacaa ccagacgttc agcaaggcac gcgtggaggc
     2041 gttccaccag gcgctgaccg cgctcggcga ggagagcccc tacgccgagt ggctcgagaa
     2101 ctggacgcgc cccgaccggg cggtcaccac ccgcgtcgag tgctccgcgt ggttcgaccg
     2161 cagggatgcc gcgctggtcg cccacgcgac ccaggtcgac cctgacggtg cgttcttccg
     2221 tgtgccgcgc gaggtgcagg tgcaggtctg gcccaccgag gactacgagg cggcgctgtc
     2281 cttcgtgccg atcgccccgg acgaggacga cctgttcgcg ggcctgccgg ccgacgtgcg
     2341 ggccgccgac gcgttggcca cctcggggaa cctgaccatc gtccacgaca cgcgtacgga
     2401 gcgagactca tgaccgatgc accctcagcc gccgtcgcac cgggcttctt cgcgttcgtc
     2461 gccttcttca tcatggcgat ggtcctgtgg ttcctcatgc ggaacatgaa tgcccggatg
     2521 cgccggatgt cctatcgcca gcaggcggcc caccgtggcc gcgacgaggc ccaggccggc
     2581 ccgggcgacg tttccgacag ccgcaggaac gacgacgaca gggcggatga ccgctcaccc
     2641 gcggacgaca gcagctccaa ggacaccggc tccagcgaca gcggtggctc ggacggcgga
     2701 ggcggcggcg ggagcgactg acccccgcga cgaagtcggg gtcaggcggg ggcgctcagg
     2761 gcgagcgagg cggccacgaa gtgcgacccg aacgccagga tcgtgagcgt gtggaagacc
     2821 tcgtggaagc cgaaccagcg cggtgagatg tccggcttct tcgtgccgta gacgatggcg
     2881 ccggcgctgt agagggctcc accgatgacg atccacgtca tgaccgcggc gccggcgtgc
     2941 tcgagcagcg gcccgaagtt gaagaccgcc acccagccga gcatcacgta cgccgggacg
     3001 tacagccagc gcggagcgtg cgaccagaac acccggaaca gcaccccgat gatcgcgccg
     3061 gcccagacga tggtgagcat gtggcgggcc tggtccgggg gtaggagcag ggcgaacggc
     3121 gtgtaggtgc cggcgatgat gaggaagatg ttcgagtggt cgaagcgccg caggaacgcc
     3181 cccatgcgcg gagaccacgt cccgcggtgg tagacggccg aggtgccgaa cagcagcacc
     3241 gcggtgccgc cgaagatcgc cgccgagacc ttgccggtcg tcgtggttgc caggaccacg
     3301 aggacgatcc cggcgatcag cgccaccggc accatgccgg cgtgcagcca gccgcgcagc
     3361 ttgggcttca cgtggtcgaa ggcggcctcg acgcgctgct cgagctcttc gacccgctgt
     3421 tcgagggcct cgcgacggct ctggtccggg gtcgcgggcg ggtcgagcgg agtcatgagt
     3481 cgaatctaac ttacgcaagc gtaggttggc gaggcccgca gccgtgactc tcgacgcagg
     3541 ccgggacggg aacaccattg cctcgccgcc cgttctgggg tccggcacac cccgctaccc
     3601 tccgcaagaa ggccccctgc gggagtagcg tgaccctgtc gtccgcgaga ccgcgacggc
     3661 cgggcggtga tggagaggca ggcgagcatg gcgctgagtg acgtgctcta cgcggcatac
     3721 gcgcgccgcc tgctgcgtga gctcgagccg tcctcggtgc cgcgccacgt cggggtcatg
     3781 ctcgacggca accgtcggtg ggcccgggcc cgcggtgccg gagccagtga gggtcaccag
     3841 gcaggcgccg acaacatcga gaacctgctc tcctggtgcg acgaggtcgg cgtcgacgtc
     3901 gtcacgctgt ggctgctgtc caccgacaac ctcaaccggc ccgaggacga gctgcggcca
     3961 ctgctcggca tcatcgaggc agccgtcgac acgctggcgg ccacccaccg ctggcg
//
LOCUS       DAOSOC010000200         3996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_22689_length_3996_cov_7.636640, whole genome shotgun sequence.
ACCESSION   DAOSOC010000200 DAOSOC010000000
VERSION     DAOSOC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22689_length_3996_cov_7.636640"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PL014_11675"
     CDS             complement(<1..384)
                     /locus_tag="PL014_11675"
                     /inference="COORDINATES: protein motif:HMM:NF015862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOT57795.1"
                     /translation="MTPLHLPSIASLEQEERDLVLPRFTHDEVWLLGSRLAERARRES
                     LPVMIDIRRPGSILFRAAMSGTTGDQDCWVERKSATAFRFEASTLLVGLRLEADKRDP
                     FTDGWLDAVRYTLAGGAVPIRVAGAG"
     gene            complement(381..1028)
                     /locus_tag="PL014_11680"
     CDS             complement(381..1028)
                     /locus_tag="PL014_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57796.1"
                     /translation="MRPSRLLAARVVAVVLAVGVSVSACTDAPTAGDAASGGHDAAGH
                     GASASPSPGESAGHEHGAEEEPAAATTGPPRPADPALGCGTTVQPDGEQVTRYCNEGL
                     ATFIAADGEGTDVKGASCEQRGVIFLAHFGANYSDESSGRGEYLGLALEDMPAKEGRA
                     SIYALEMTLAGYRQSLSKATVDVTRKGDVVDFHLVGALADGRDVSVTASCHTHKA"
     gene            complement(1044..2420)
                     /locus_tag="PL014_11685"
     CDS             complement(1044..2420)
                     /locus_tag="PL014_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57797.1"
                     /translation="MAAAAAMVALPTVAYACTDDFKHEMEMAAAREQAATGKAAKAAA
                     VAEGEMVTTAPTGAFDNVEFIGQLPEAVGATAINFIDYGSKKKVMFVTGRFGLKSYDV
                     SNPEKPVALDSIDMPGFWQNEDMDVDPNRKLVYMARDPRAFGQNQATGESGVYIVNAK
                     DPSKLEVLSYTVVPAGHTTTCINDCQYLWSGGPRPADWQPEDWEGRPIFVVDVRNPKK
                     PKVSEQPVDLGRNDGVTDYAHDVQIDEAGVAWVSGAGGIRGYWTKGRQWDPVQKKYRV
                     ATAFDPVPYAGGKFYQSNQETWGGSLAHNMARPLEALGDHEAGSLALVTIENFTSTCE
                     TDGRLLIVDLADSREGQAWSSTPEDPYRLPVVSEWSPFGKPGSLPNAGCSAHYFQLRD
                     GVLAQSYYGQGTHFIDVSDPENPEQVGYFRADGGSNWAPYWNGDVMYIADGRRGVDIV
                     RPIIPAKG"
     gene            complement(2622..3890)
                     /locus_tag="PL014_11690"
     CDS             complement(2622..3890)
                     /locus_tag="PL014_11690"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOT57798.1"
                     /translation="MSEVDPAGASSVDMARAERALDDLLDLGGVAAPHELPRLVDDTA
                     LALGAVAAVIHLVDLQQVALTPFPEPGHPPVTELPAPLVIDSTIAGRVYQHDEVIVQT
                     TSDAGAPVTVWLPLRDGADRLGVLAVTLQEPSLVEDADSPTSRLLRRLAAITSDLIAQ
                     RSRYGDSIIAATRTTEVTLAAEIQWGLLPPLTFESTQVAVAGLLEPAYDVAGDSIDYA
                     VDPGVIHLAIFDGMGHGLRSAQLAILAVSAYRNARRSGHSLTTTASDVDQAVEAGFGG
                     EAYTTSVLAQLDTDTGYLSWVNAGHPEPLLLRGGALVRSLHTGPDLPFGIGLREGDAP
                     YEVGSEQLQPGDHVLFYTDGVVEGHAPGGEQFGVERLVDLLARNVADNLSAAESMRRV
                     GRALVAHHAAHLADDASILLVHWPGPGDGQ"
BASE COUNT          583 a         1345 c         1453 g          615 t
ORIGIN      
        1 cccggcgccg gcgacccgga tcgggaccgc acctccggcg agggtgtagc gcacggcatc
       61 caaccacccg tccgtgaacg gatcacgttt gtcggcttcg aggcgcaacc cgacgagcag
      121 ggtgctcgcc tcgaagcgga aggccgtcgc ggacttgcgc tcgacccagc agtcctggtc
      181 gccggtcgtg cccgacatcg cggcccggaa gaggatgctc cctgggcgcc ggatgtcgat
      241 catcaccgga aggctctcgc ggcgagcgcg ctcggccagg cgcgagccga gcagccacac
      301 ctcgtcgtgg gtgaaccgag gcaggacgag gtcgcgctcc tcctgctcga gcgaggcgat
      361 cgacggcagg tggagggggg tcatgccttg tgggtgtgac agctcgcggt gacggagacg
      421 tcgcgaccat cggcgagggc gccgacgagg tggaagtcga cgacgtcgcc cttgcgggtc
      481 acgtcgacgg tggccttgga cagtgactgc cggtatccgg ccaacgtcat ctccagcgcg
      541 tagatggagg cgcggccctc cttggctggc atgtcctcca gggccaggcc gaggtactcc
      601 ccgcgacccg aggactcgtc ggagtagttc gcgccgaagt gggcgaggaa gatcacgcca
      661 cgctgctcgc acgaggcgcc cttgacatcg gtgccctcgc catcggcggc gatgaacgtc
      721 gccaacccct cgttgcagta gcgggtcacc tgttcgccgt cgggctgcac cgtcgtgccg
      781 cagcccaacg cggggtcggc ggggcggggc gggcccgtcg tggcggctgc gggttcctct
      841 tccgcgccgt gctcgtggcc tgcggactcg cctggtgacg ggctcgctga cgcgccgtgg
      901 ccggctgcgt catgaccgcc ggatgccgca tcgcccgcgg tgggggcgtc ggtgcacgct
      961 gacaccgaca cccccaccgc gagaacgacg gcgacgaccc gagccgcaag cagtcgggac
     1021 ggcctcacgg ctcgggtcat cgatcagccc ttcgcgggga tgatcggacg aacgatgtcc
     1081 acgccacggc gaccgtcggc gatgtacatg acgtcaccgt tccagtacgg cgcccagttc
     1141 gacccgccgt cggcacggaa gtagccgacc tgctcggggt tctcggggtc gctcacgtcg
     1201 atgaagtggg tgccctgacc gtagtaggac tgggccagca ctccgtcacg cagctggaag
     1261 tagtgcgccg agcaaccggc gttgggcagg ctgccgggct tgccgaacgg gctccactcc
     1321 gagacgaccg gaaggcggta cgggtcctcg ggagtcgagc tccaagcctg tccttcccgg
     1381 ctgtccgcga ggtcgacgat gagcaggcgc ccgtcggtct cacacgtgga ggtgaagttc
     1441 tcgatggtca ccagggccag cgagccggcc tcgtggtcac cgagggcctc gaggggacgc
     1501 gccatgttgt gggccagcga cccaccccac gtctcctggt tggactggta gaactttcca
     1561 ccggcgtacg ggaccgggtc gaacgcggtc gcgacgcggt acttcttctg caccgggtcc
     1621 cactgacggc ccttggtcca gtagccgcgg atgccaccgg cacccgagac ccaggccact
     1681 ccggcctcgt cgatctggac gtcgtgcgcg tagtcggtga cgccgtcgtt gcggccgagg
     1741 tcaaccggct gctcggacac cttcggcttc ttggggttgc ggacgtcgac cacgaagatc
     1801 ggacggccct cccagtcctc gggctgccag tcggctggcc gcggaccgcc ggaccacagg
     1861 tactggcagt cgttgatgca cgtcgtcgtg tggccggcag gcaccacggt gtagctgagc
     1921 acctcgagct tgctcgggtc cttggcgttg acgatgtaga cgccggactc gccggtcgcc
     1981 tggttctggc cgaaggcccg cgggtcgcgt gccatgtaca cgagcttgcg gttcgggtcg
     2041 acgtccatgt cctcgttctg ccagaagccc ggcatgtcga tggagtcgag cgcgacgggc
     2101 ttctcggggt tggacacgtc gtacgacttc aggccgaagc ggccggtcac gaacatgacc
     2161 ttcttcttcg agccgtagtc gatgaagttg atcgcggtgg cgccgacggc ctcgggcagc
     2221 tgcccgatga actcgacgtt gtcgaacgcg ccggtcgggg ccgtggtgac catctcgccc
     2281 tcggcgacgg ctgcagcctt ggccgccttg ccggtggctg cctgctcacg cgcggccgcc
     2341 atctccatct cgtgcttgaa gtcgtcggta caggcgtagg caacggtggg cagcgccacc
     2401 atggccgccg cggcagccat ggtgatcacg ctgcgccgca gtgctgaccg ccccggttgg
     2461 ggtgttgcat gcagaggcat tctcgtcctt tcacacacaa gggaaccgtc ctgggtgtgg
     2521 gacggtgcgc gaacactatt gcccgatggt gcgcaaaagg gttttccgta caacaaactt
     2581 tcgggaacat tcgctctgct ggtcgccggg gttcgccgag gtcactggcc gtcgcccggg
     2641 cccggccagt gcacgagcag gatcgacgcg tcgtcggcga ggtgggccgc gtggtgggcg
     2701 accagggccc gcccgacgcg gcgcatcgac tctgccgcgg acaggttgtc cgccacgttg
     2761 cgtgcgagca ggtcgacgag ccgctcgacg ccgaactgct cgccgccggg ggcgtgcccc
     2821 tcgaccacac cgtcggtgta gaagagcacg tggtcgccgg gctggagctg ctcggacccg
     2881 acctcgtagg gggcgtcgcc ctcgcgcagg ccgatgccga acggcaggtc ggggccggtg
     2941 tgcaggctgc gcacgagcgc accgccgcgc agcagcagcg gctcggggtg accggcgttg
     3001 acccagctga ggtagccggt gtcggtgtcg agctgggcca ggaccgaggt ggtgtacgcc
     3061 tcgccaccga accccgcctc gacggcctgg tcgacgtcgg atgccgttgt cgtcagggag
     3121 tgcccgctgc ggcgggcgtt gcggtaggcc gacaccgcga ggatggcgag ctgcgcgctg
     3181 cgcagcccgt ggcccatgcc gtcgaagatg gcgaggtgga tgaccccggg gtccaccgcg
     3241 tagtcgatcg agtcacccgc gacgtcgtag gccggctcga gcaaccccgc aaccgccacc
     3301 tgcgtggact cgaacgtcag cggcgggagc aggccccact ggatctccgc cgcgagcgtg
     3361 acctcggtgg tgcgggtcgc ggcgatgatg ctgtcgccgt accgcgagcg ctgggcgatg
     3421 aggtcgctcg tgatggcggc gagtcggcgc agcaggcggg aggtggggga gtcggcatcc
     3481 tcgacgagcg acggctcctg cagcgtcacc gccagcaccc ccagacggtc cgcaccatcg
     3541 cgcaacggca gccacacggt gaccggtgca ccggcatccg acgtggtctg cacgatgacc
     3601 tcgtcgtgct ggtagacccg accggcgatc gtcgagtcga tgacgagcgg ggcggggagc
     3661 tccgtcacag gtgggtgccc gggctcgggg aagggggtga gggcgacctg ctggaggtcg
     3721 acgaggtgga tgacggccgc gacagcgccc agcgcgagcg cggtgtcgtc gacgaggcgc
     3781 gggagctcgt ggggtgctgc gaccccgccc aggtcgagca ggtcgtcgag ggcgcgctca
     3841 gcccgggcca tgtccacgga cgatgctccg gccgggtcca cctcgctcac gacaggcaac
     3901 ctagcgcagc ccgcggccgg tcaaccctcg acgaggacga tccgaccctc gacgtgaccg
     3961 accctcccgg tccacgatgg tcacctcccg ccccgg
//
LOCUS       DAOSOC010000201         3989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_22746_length_3989_cov_9.947382, whole genome shotgun sequence.
ACCESSION   DAOSOC010000201 DAOSOC010000000
VERSION     DAOSOC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22746_length_3989_cov_9.947382"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..2499
                     /locus_tag="PL014_11695"
     CDS             34..2499
                     /locus_tag="PL014_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT57799.1"
                     /translation="MSTALPARARVVIIGGGVIGASIAYHLAHLGITDVLVLERDRLT
                     SGTTWHAAGLMTCFGSTSETNTRMRLYSRDLYARLEAETGVATGFKPVGLIEAAADAD
                     RLEEYRRVAAFQRHIGLEVEEIGPKEMADLFPLARTDDLLAGFHVPGDGRVNPVDVTM
                     SLARGARNLGVRIVEGVRVEQVVTRPRGLVEEVTGVRTDAGDVECDVVVNCAGMWARE
                     LAERNGVVVPNQAAEHYYLITEPIDGMSPDAPVFEDPASYGYYREEGGGMMVGLFEPV
                     AAAWNVDGIPRDFSFGEIAPDWDRMGPFVERAIARVPVVETVGIRTFFCGPESFTPDL
                     APAVGEAPGIHGYFVCAGLNSVGILSAGGMGRIVAQWVADGHPDVDVTGFDVARFRDW
                     QLNPTHRAQRTAEILGTVYAAHTPGIELTSARGEFTSPVHDRLVAEGARLRDVSGWES
                     AAWYAGRGQTPTDEPGWGHHPWFANWRAEHEAVREAVGLMDMSFMAKFAVRGPDSGVV
                     LDRLSAGAVNERDGVITYTQWLGEDGGIRADLTVTRLAADDFLVVVSDTAHGSALGQL
                     RRAVGDADATVEDVTTELALLTVQGPSSRATLAQAAPGADWSSEAFPFRAARRVDIAG
                     VEVLAIRITYLGELGWELYAPASHAGEVWDAVLAAGAAHGIRPVGLKALSSLRMEKGY
                     RDFGHDIDNTDDVYGVGLGFAVALDKPGGFVGREATLAAKERGTPHHRLVQVLLRDPQ
                     PLLFHAEPVLRDGVVVGEVRSASYGWTLGGAVGLAFVGGDGPVTPQWLADGQWEVDVA
                     GTRHPAVVSLRPMYDPTSARVTG"
     gene            2523..2801
                     /locus_tag="PL014_11700"
     CDS             2523..2801
                     /locus_tag="PL014_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57800.1"
                     /translation="MPRANRRRRDLESRPLSGASEVGTQTYSGRPWHVRQLSGASSTR
                     EYRCPGCHQGIAVGTPHVVVWPAQGVGGIDERRHWHTRCWSARHAARP"
     gene            complement(2816..3865)
                     /locus_tag="PL014_11705"
     CDS             complement(2816..3865)
                     /locus_tag="PL014_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT57801.1"
                     /translation="MGRPLNRHSPFYIGFFGATGAIIAIGLWRLVGQITTTLTLLVVS
                     LFIALALSPIVDGLVSRGVRRAAAVAAVFSGLLVIFGVILAVVIPPVALEGAELARQA
                     PGYVEQLLDARWVRDLDANYDVLDTLQAEIRARAMDQSFIQGVLGGIFGVGRAVLNGT
                     FQVFTVLVLTLYFLGSLPRVKQAGYALVPASRRSRVEPLSEEIMRRVGSYALGQVSIA
                     TLNGVLSYLMMTLVGIPYAAVLAVTVGLLGLIPMIGATLGAVLVCAIAFVSEPNTALI
                     AGVYYLIYQQVENYLVAPRIMQRTVSVPGPVTIVAALAGGALLGVLGALLAIPVAAGL
                     LLLYEEVLVPRQNRA"
BASE COUNT          577 a         1373 c         1481 g          558 t
ORIGIN      
        1 ccgagccggg cgcggggtgg ataggttggg cgggtgagca cagcattgcc ggcgcgggcc
       61 cgggtcgtca tcatcggagg aggcgtcatc ggcgcctcga tcgcctacca cctggcgcat
      121 ctgggcatca ccgatgtcct cgtgctcgaa cgtgaccggc tgacgtccgg cacgacgtgg
      181 cacgcggccg gcctgatgac ctgtttcggc tccacgagcg agacgaacac ccggatgcgg
      241 ctgtactcgc gtgatctcta tgcccgcctg gaggcggaga cgggtgtggc gacggggttc
      301 aagccggtcg ggctcatcga ggcggctgcg gacgcggacc ggctcgagga gtaccgccgg
      361 gtcgcggcgt tccagcgcca catcggcctc gaggtcgagg agatcggccc gaaggagatg
      421 gccgacctct tcccgctggc gcgcaccgac gacctgctcg cgggcttcca cgtgccgggc
      481 gacggccggg tcaacccggt cgacgtcacg atgagcctgg ccagaggggc ccgcaacctc
      541 ggggtcagga tcgtcgaggg ggtgcgggtc gagcaggtcg tcacccgccc gcgcgggctc
      601 gtcgaggagg tcaccggggt ccgcacggat gccggtgacg tcgagtgcga cgtcgtcgtc
      661 aactgcgcgg gcatgtgggc ccgtgagctc gccgagcgca acggcgtcgt cgtgccgaac
      721 caggcggccg agcactacta cctcatcacc gagccgatcg acggcatgag cccggacgcg
      781 ccggtgttcg aggacccggc ctcctacggc tactaccgcg aggagggcgg cggcatgatg
      841 gtcgggctct tcgagccggt cgccgccgca tggaacgtcg acggcatccc acgcgacttc
      901 tccttcggtg agatcgcccc cgactgggac cggatgggcc ccttcgtcga gcgcgccatc
      961 gcgcgcgtac cggttgtcga gaccgtcggc atccgcacgt tcttctgcgg gcccgagtcc
     1021 ttcacccccg acctcgcgcc ggcggtgggt gaggctcccg gcatccacgg ctacttcgtg
     1081 tgtgcggggc tgaactccgt cggcatcctg tcggctggcg ggatgggccg catcgtcgct
     1141 cagtgggtcg ccgacggcca ccccgacgtc gacgtcaccg ggttcgacgt cgcccgcttc
     1201 cgggactggc agctcaaccc gacgcaccga gcgcagcgca ccgccgagat cctcggcacc
     1261 gtgtatgccg ctcacacgcc gggcatcgag ctcacctctg cgcgtggcga gttcacctca
     1321 cccgtgcacg accggctggt cgcggagggt gcccgtctgc gtgacgtcag cggctgggag
     1381 tccgcggcct ggtacgccgg gcgtgggcag acccccaccg acgaaccagg ctgggggcac
     1441 cacccgtggt tcgcgaactg gcgcgccgag cacgaggcgg tgcgcgaggc cgtcgggctc
     1501 atggacatgt cgttcatggc caagttcgcg gtgcgcgggc ctgattccgg cgtggtgctc
     1561 gaccgcctga gcgctggcgc ggtcaacgag cgcgacgggg tcatcacgta cacccagtgg
     1621 ctcggggagg acggtggcat ccgggcagac ctcacggtga cccgcttggc ggcggacgac
     1681 ttcctcgtcg tcgtgtccga caccgcgcac gggtcggcgc tgggccagct gcggcgggcg
     1741 gtcggagacg ccgacgccac cgtcgaggac gtcacgaccg agctcgcgct gctcaccgtg
     1801 cagggcccca gctcccgcgc gaccctcgcg caggctgctc ccggcgctga ctggtcgagt
     1861 gaggccttcc cgttccgggc ggcgcgccgc gtcgacatcg ccggtgtcga ggtgctcgcc
     1921 atccgcatca cctacctcgg tgagctgggg tgggagctgt acgcgcccgc ttcgcacgcg
     1981 ggcgaggtgt gggatgccgt cctcgcggcg ggtgctgcgc acggcatccg acccgtgggt
     2041 ctcaaggcgt tgtcgagcct gcggatggag aagggctacc gcgacttcgg ccacgacatc
     2101 gacaacaccg acgacgtcta cggcgtcggg ctcggtttcg ccgtcgccct cgacaagccg
     2161 ggcgggttcg tcgggcgcga ggccaccctg gcggccaagg agcggggcac accgcaccac
     2221 cggctcgtcc aggtgctgct gagagacccc caaccgctgc tcttccacgc cgagccggtg
     2281 ctacgcgacg gtgtcgtcgt cggggaggtg cgctcggcgt cgtacggctg gaccctcggc
     2341 ggcgcggtgg gcctggcgtt cgtcggcggc gacgggccgg tgacgccgca gtggctcgcc
     2401 gacgggcagt gggaggtcga tgtcgccggg acccggcatc cggccgtggt gtcgttgcgt
     2461 ccgatgtacg acccgacgag cgcgcgcgtc acgggctgac gacgtctgga agcatgggcg
     2521 gcatgcccag ggccaaccgc cgccgccgcg acctcgagtc ccggccgctc tcgggcgcca
     2581 gcgaggtcgg tacccagacc tactcgggcc gtccgtggca cgtccgccag ctgtcggggg
     2641 cgtcatcgac ccgggagtac cggtgcccgg ggtgtcatca gggcattgcc gtcggtacgc
     2701 cgcacgtcgt cgtgtggccg gcccaggggg tcggcggtat cgatgagcgg cggcactggc
     2761 acacccgctg ctggtcggcc cggcacgccg cccgcccctg agccgtaggg ggcggtcagg
     2821 cgcggttctg gcgcgggacc agcacctctt cgtagagcaa caggagcccg gcggccaccg
     2881 ggatcgcgag cagggcgccg agcaccccga gcagcgcgcc gccggcaagg gcggcgacga
     2941 tcgtcaccgg ccccggtacc gacaccgtgc gctgcatgat ccgcggcgcc acgaggtagt
     3001 tctcgacctg ctggtagatc aggtagtaca cgcccgcgat gagcgccgtg ttcggctcgg
     3061 acacgaacgc gatggcgcac acgagcaccg ccccgagggt ggcaccgatc atcgggatga
     3121 gcccgagcag gccgacggtc acggcgagca ccgcggcgta cgggatgccg acgagggtca
     3181 tcatcaggta cgacagcacc ccgttcaggg tggcgatgga gacctggccc agggcgtagg
     3241 agcccacccg gcgcatgatc tcctccgaca gcggctcgac gcgggaccgc cgcgaggccg
     3301 gcacgagggc gtaaccagcc tgcttgacgc gcggcagcga ccccaggaag tacagcgtca
     3361 gcacgagcac cgtgaagacc tggaaggtgc cgttgagcac ggctctgccc accccgaaga
     3421 tgccgccgag caccccctgg atgaacgact ggtccatggc ccgcgctcgg atctcggcct
     3481 ggagggtgtc caggacgtca tagttcgcgt cgaggtcgcg cacccagcgg gcatcaagga
     3541 gctgctcgac gtatccgggg gcctgtcgcg ccagttccgc cccttcgagg gcgacgggcg
     3601 ggatgaccac ggccaggatc acgccgaaga tcacgagcaa cccgctgaag acggcggcca
     3661 cggctgcggc ccggcgcacg ccccgcgaca cgaggccgtc gacgatcggg ctgagtgcga
     3721 gcgcgatgaa gagggagacg acgagcagcg tcagcgtcgt cgtgatctgc ccgacgagcc
     3781 gccacaggcc gatggcgatg atcgcccccg tggcgccgaa gaagccgatg tagaacggcg
     3841 agtggcggtt gagcgggcgg ccgatgacac cgtagggcgt gaccgtcggc tgggtcgggt
     3901 cgacgacgaa cggcgaggat gccgaggtgc gggattcctc ggagctcccg gtgggggttt
     3961 ggggctggac gaccgcgtca ccgtcgccg
//
LOCUS       DAOSOC010000202         3954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_22990_length_3954_cov_9.074122, whole genome shotgun sequence.
ACCESSION   DAOSOC010000202 DAOSOC010000000
VERSION     DAOSOC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22990_length_3954_cov_9.074122"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PL014_11710"
     CDS             complement(<1..800)
                     /locus_tag="PL014_11710"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOT57802.1"
                     /translation="MTDAPDRTAADAPDVPDAPDAPAAPATPKAGRDLRAAVGVGLLL
                     GGLVIGTLIVDARAFLVVVIAAICVGAWELRRAIATKGINIPLVPLLLGVAGMLVSAY
                     ARGAEALVVTFGLTIIGILIWRVADGVAGAARDLSASALVTFYPVFLAGFAALMLAAP
                     DGRQRIVVFILVTVFSDIGGYAVGVKFGKHPMAPSLSPKKSWEGFAGSVLSCALVGAI
                     SLPLLLDGVWWAGAVLGVVAAAAATLGDLIESSIKRDLGIKDMSTILPG"
     gene            complement(816..1373)
                     /gene="frr"
                     /locus_tag="PL014_11715"
     CDS             complement(816..1373)
                     /gene="frr"
                     /locus_tag="PL014_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT57803.1"
                     /translation="MIDETLFEAEEKMEKTVEAAKEAFAGIRTGRAHPGMFQKVLVDY
                     YGAPTPLQQLASFQTPEARTIIISPFDKSSMHAIEKAIQVADLGVNPSNDGNIIRCVM
                     PVLTEERRREYVKMAHSQAEDAKVSVRNVRRSAKTTLEKLVKDGEVGEDEGARAEKEL
                     EAVTKRHVDQIDELLKHKEAELLEV"
     gene            complement(1477..2235)
                     /gene="pyrH"
                     /locus_tag="PL014_11720"
     CDS             complement(1477..2235)
                     /gene="pyrH"
                     /locus_tag="PL014_11720"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT57804.1"
                     /translation="MTTHAQTQAAPNASGAASPRRVLLKLSGEVFGGGKVGVDPDVVR
                     GIARQIADAVREGIEVAVVVGGGNFFRGAELQQKGMDRSRADYIGMLGTVMNCLALQD
                     FLEKEGIDTRVQTAITMGQVAEPYIPRRAIRHLEKGRVVIFGAGAGMPYFSTDTVSAQ
                     RALECRCDMVLMSKSGVDGVYDADPRTNPDARKLEHVTFEEALRTGLKVVDAAAFSLC
                     MDNKLPMVVFGMEGDGNITRALRGERIGTLVSAG"
     gene            2398..2931
                     /locus_tag="PL014_11725"
     CDS             2398..2931
                     /locus_tag="PL014_11725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT57805.1"
                     /translation="MWPVTESRTVYANAWIEVVEDAVVRPDGSDGIYGVVHLRHPAVF
                     VVAVTDDDEVVLVTVDRHTTGPSVEVPAGGSDGPDTLAGAQRELAEETGLQAATWREI
                     GRMNALNGVCVAPEVVYLATGLSPVDGAEQEVEGISEVRRVPWGEVLAMLARGDITDG
                     ETVAALMYAALARGRVT"
     gene            complement(3036..3866)
                     /gene="tsf"
                     /locus_tag="PL014_11730"
     CDS             complement(3036..3866)
                     /gene="tsf"
                     /locus_tag="PL014_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT57806.1"
                     /translation="MANYTAADIKALREKTAAGMMDVKKALDEADGDMAKAEEILRVK
                     GLKGVTKREGRTASNGLVTANAGAGEGTLVEVLCETDFVAKGERFGALAAQVLDQAVA
                     TKSADAEALLASTLEDGRTVKELLDEANATIGEKIEVKRVARLEGEHVVSYLHKTSPD
                     LPAQIGVLVSTAGGDEQTAKDVAMHIAAFSPSVLTREEIDAATVENERRVAEATAKEE
                     GKPEAALPKIVEGRVNGFFKENVLLEQPFAKDAKKTVAKVLSEAGADAKGFARFRVGS
                     "
BASE COUNT          555 a         1350 c         1400 g          649 t
ORIGIN      
        1 ccgggcagga tcgtgctcat gtccttgatg ccgaggtcgc gcttgatgct ggactcgatg
       61 aggtcaccga gggtggcagc ggccgcggcg acgacgccga gcacggcgcc agcccaccag
      121 acgccgtcca ggagcagggg cagcgagatg gcaccgacga gtgcgcacga cagcaccgaa
      181 ccggcgaacc cttcccacga cttcttgggg ctgagcgacg gcgccatcgg gtgcttgccg
      241 aacttgaccc ccaccgcgta gccaccgatg tcggagaaga cggtgacgag gatgaacacg
      301 acaatgcgct ggcgaccgtc aggtgccgcg agcatgaggg cggcgaaccc ggcgaggaac
      361 acggggtaga acgtcacgag ggctgatgcg ctcaggtcac gagccgcccc ggcgaccccg
      421 tcggccaccc gccagatgag gatgccgatg atcgtcaggc cgaacgtcac gaccaacgcc
      481 tcggccccgc gcgcgtaggc agacaccagc atgccggcca ccccgaggag gagcggcacc
      541 agcgggatgt tgatgccctt ggtggcgatg gcccgacgca gttcccaggc ccccacgcag
      601 atcgcggcga tcacgacgac gagaaaggcc cgcgcatcga cgatgagcgt gccgatgacc
      661 agcccgccga gcagcagccc caccccgact gctgcccgaa ggtcgcgccc ggccttcggg
      721 gtggccggcg ccgccggagc atcaggtgcg tcagggacgt ccggggcgtc ggcggcagtg
      781 cggtcggggg cgtcggtcat cgtcgtggtg cgaggtcaga cctcgagcag ctcggcttcc
      841 ttgtgcttga gcagctcgtc gatctggtcg acgtggcgct tggtgacggc ctcgagctcc
      901 ttctcggcgc gcgccccctc gtcctcaccg acctcgccgt ccttgacgag cttctccagg
      961 gtcgtcttgg cgctgcgtcg cacgttgcgc accgacacct tcgcgtcctc ggcctgcgag
     1021 tgcgccatct tcacgtactc acgacgccgc tcctcggtga gcaccggcat gacgcagcgg
     1081 atgatgttgc cgtcgttgct ggggttgacg cccaggtcag ccacctggat cgccttctcg
     1141 atggcgtgca tcgagctctt gtcgaacggg ctgatgatga tggtgcgcgc ctcgggggtc
     1201 tggaacgacg cgagctgctg gagcggggtc ggcgcaccgt agtagtccac gagcaccttc
     1261 tggaacatgc cggggtgcgc gcgaccggtg cggatgccgg cgaaagcctc cttggcggcc
     1321 tcgacggtct tctccatctt ctcctcggcc tcgaacaagg tctcgtcgat catctgcggt
     1381 ggttctcctt cggtatgccg cgctaccggc gcggcgggtc tggtcccagt gtgtcagggt
     1441 cgcgggccgc ccggtgaccg ccgcgcgagg agcgcctcag cccgcggaga ccagcgtccc
     1501 gatgcgctcg ccccgcaggg cacgggtgat gttgccgtcg ccctccatcc cgaacacgac
     1561 catgggcagc ttgttgtcca tgcagaggct gaacgcggcg gcgtcgacga ccttcagacc
     1621 ggtgcgcagc gcctcctcga aggtcacgtg ctcgagcttg cgggcgtcag gattggtgcg
     1681 agggtctgcg tcgtacacgc cgtcgacgcc gctcttgctc atgagcacca tgtcgcagcg
     1741 gcactccagc gctcgctggg cgctcacggt gtcggtggag aagtacggca tccccgcccc
     1801 ggcaccgaag atgacgaccc gccccttctc gaggtggcgg atcgcgcgac gcgggatgta
     1861 gggctcggcg acctggccca tcgtgatggc ggtctgcacc cgggtgtcga tgccctcctt
     1921 ctcgaggaag tcctggagag cgaggcagtt catcacggtg ccgagcatgc cgatgtagtc
     1981 ggcgcgcgag cggtccatgc ccttctgctg gagctcggca ccgcggaaga agttgccccc
     2041 acccacgacg acggcgacct cgatgccctc acggacggca tccgcgatct ggcgggcgat
     2101 gccgcgcacg acgtcggggt cgaccccgac cttgcctccg ccgaacacct caccggagag
     2161 cttgagcagc acccgtcgcg gagaggctgc gcccgacgca ttcggtgcgg cctgggtctg
     2221 ggcgtgcgtg gtcacggtgc ctcctcggca gtggttcgcg gtcgctcgat cctgccacag
     2281 cgccggtgtt ccacccggac ggggaccggg tcggatgccg ttttcgtccc cgtcgtggat
     2341 gatcctggct cgacctcggg cggtgcgccc actccacacc cgtggcaggc tgacgccgtg
     2401 tggcctgtca ctgagtcccg gaccgtgtac gccaacgcgt ggatcgaggt cgtcgaggat
     2461 gccgtggtgc gccccgacgg gagcgacggc atctacggcg tcgtgcacct gcggcatccg
     2521 gcggtgttcg tcgtggcggt gaccgacgac gacgaggtgg tgctcgtgac ggtcgaccgg
     2581 cacacgaccg ggccttccgt cgaggtgccc gccggtgggt ccgacggccc ggacaccctc
     2641 gcgggggcgc agcgggaact cgccgaggag accggcctcc aggcggcgac ctggcgcgag
     2701 atcggccgga tgaacgccct caacggcgtc tgcgtggccc ccgaggtcgt gtacctggcg
     2761 acgggcctgt cccccgtgga cggtgccgag caggaggtcg agggcatcag cgaggtgcgg
     2821 cgggtgccgt ggggtgaggt gctcgccatg ctcgctcgcg gtgacatcac cgacggtgag
     2881 accgtggccg ccctcatgta cgcagccctg gcccggggcc gcgtcacctg acgccgcgcc
     2941 gtcgatccct cgacgcgaac ctgccgacaa cgcacggatg ccgtccgctc aggtgagcgg
     3001 acggcatccg gtgatgctgg tggtgcggtg cctcgtcagc taccgacccg gaagcgcgcg
     3061 aagcccttgg cgtcggcgcc ggcctcggac agcaccttgg cgacggtctt cttggcgtcc
     3121 ttggcgaacg gctgctcgag caggacgttc tccttgaaga agccgttcac gcggccctcg
     3181 acgatcttcg gaagggcggc ctcgggcttg ccctcctcct tcgcggtggc ctcggcgacg
     3241 cgacgctcgt tctcgaccgt cgcggcgtcg atctcctcac gggtgagcac gctcgggctg
     3301 aacgcggcga tgtgcatcgc cacgtccttg gccgtctgct cgtcgccacc ggcggtggag
     3361 acgaggacac cgatctgcgc gggcaggtcg gggctcgtct tgtgcaggta ggacacgacg
     3421 tgctcgccct cgaggcgggc aacgcgcttg acctcgatct tctcgccgat ggtggcgttg
     3481 gcctcgtcga ggagctcctt gacggtgcgg ccgtcctcga gcgtcgaggc cagcagggcc
     3541 tcggcgtcgg cggacttggt ggcaacggcc tggtcgagga cctgcgcggc cagggcgccg
     3601 aagcgctcac ccttggccac gaagtcggtc tcgcacagaa cctcgacgag ggtgccctcg
     3661 ccggcgccgg cgttggcggt gaccagcccg ttggaggccg tacggccctc gcgcttggtc
     3721 acacccttca ggcccttgac gcgaaggatc tcctcggcct tggccatgtc gccatcggcc
     3781 tcgtcgaggg ccttcttgac gtccatcatt ccggcggcgg tcttctcgcg gagcgccttg
     3841 atgtcagcgg cggtgtagtt cgccatgagt ggtatcgctc tttcagctag cagttcggac
     3901 agggacgagg gggcaggctc aggcctggtc cgcgtcggcg gccggggtgg cctc
//
LOCUS       DAOSOC010000203         3914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_23285_length_3914_cov_13.388961, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000203 DAOSOC010000000
VERSION     DAOSOC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23285_length_3914_cov_13.388961"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PL014_11735"
     CDS             complement(<1..1061)
                     /locus_tag="PL014_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOT57807.1"
                     /translation="MSTIPGFWAVVPAGGAGTRLWPLSRAAHPKFLLDLTGSGRTLLQ
                     ATVDRLEPLTGDRVVVVTGAAHADAVRAQLPGLAGDQVLAEPSPRDSMAAIGLAAAVV
                     ERQDPQAVIGSFAADHVIPDTAAFESVIREAAEVAREGHLVTIGIEPTSPATGFGYIR
                     AGEALPGFATALRAVEFVEKPDAVRAAQYVASGEFRWNAGMFVVRAATLLDLLAQWHH
                     ELAAGVRAIAASPERLDELWPGLTRIAIDHAVAEPAADAGHVVVVPAPFTWDDVGDFA
                     SLAELLPPVEGEPGLRVLGSVDDVTTIDASGIVAAAGGRRVAVVGIEDVVVIDTPDAV
                     LVTTRSRAQDVKAVVDALKA"
     gene            complement(1088..1600)
                     /locus_tag="PL014_11740"
     CDS             complement(1088..1600)
                     /locus_tag="PL014_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOT57808.1"
                     /translation="MPNHGVAVTIPEPWATTLQSAREAVGDTMASAIPPHVTLLPPTA
                     IHPPQMPSFLEHLSAVTESVAPFEMVLSGTGTFRPVSPVVFVQVSRGIGECEALERAV
                     RSGPIERLLDFPYHPHVTIAHHLGEDALDRAFDELADFRCRFPVTSVELYHHDHDGVW
                     RVIDSFPLRG"
     gene            complement(1612..2691)
                     /gene="trpS"
                     /locus_tag="PL014_11745"
     CDS             complement(1612..2691)
                     /gene="trpS"
                     /locus_tag="PL014_11745"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT57809.1"
                     /translation="MSTPASPASARPRILSGMQPTSDSLHLGNYLGALVNWVGLQQDF
                     DAYFFVADLHALTVPTDPEVLRRRTRVTAAQFIAGGVDPHTSAVFCQSHVGPHPELAW
                     VLACQTAMGEMNRMTQFKDKTAKGHNANVGLFTYPVLMAADILMYDAAFVPVGEDQRQ
                     HLEITRDLAERMNARFGDVLTVPEAYILKESAKIMDLQEPTSKMSGSSSTEKGMVLLS
                     DEPTRIAKKIRSAVTDTEAVVRYDPEAKPGVSNLLVIHSVLSGTAIPALEDEFAGRGY
                     GDLKKAVADVVVEAVTPFRTRMAELLDDPAELDRILASGAERAAAVADATMSRIRDAV
                     GLLHPVGAAVAAVALAGSSAAIAAE"
     gene            2733..3911
                     /gene="galK"
                     /locus_tag="PL014_11750"
     CDS             2733..3911
                     /gene="galK"
                     /locus_tag="PL014_11750"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOT57810.1"
                     /translation="MTRAPGIHLEVFGSAPHGVWSAPGRVNLIGEHTDYNDGLVLPIA
                     LPQRTYAACSPRDDGLLRVHSAQAGATVEVDLASVGPGSPAGWTAYVAGVLWALAQDG
                     HDVRGMDVTVDSEVPVGAGLSSSAALECAVGAAASDLFGLGLLADDASRARLAAACVR
                     AENDVAGAPTGGMDQSASLLCRADHALLLDCRTGATEHVPFVLGSGEGSDAVAGHVLL
                     VTDTRAEHSLNDGQYAQRVASCDAASAALGVSSLRDVDVAGLEASLVVLSDDVLRRRA
                     RHVVTEIARVEATVAALRGGDLTEVGRLFDESHTSLRDDYEVSCAELDVSVQAARSAG
                     ALGARMTGGGFGGSSIALLPADAVDTAVAAIAQAFAERGWRAPASFAVTAGDSAHRDA
                     "
BASE COUNT          539 a         1388 c         1449 g          538 t
ORIGIN      
        1 gccttgaggg cgtcgacgac ggccttgacg tcctgcgccc gcgagcgggt ggtgacgagc
       61 acggcatccg gggtgtcgat cacgacgacg tcctcgatcc ccaccactgc cacgcggcgg
      121 ccaccggctg ccgcgacgat cccggacgcg tcgatggtcg tcacgtcgtc gaccgacccg
      181 agcacccgca ggccgggctc cccctcgacg ggcggcagca gctcggccag gctggcgaag
      241 tcgcccacgt cgtcccaggt gaagggggcc gggacgacga cgacgtgacc ggcatccgcg
      301 gccggctcgg cgacggcgtg gtcgatggcg atcctcgtca gccccggcca cagctcgtcg
      361 agccgctcgg gcgaggccgc gatggctcgc acccctgccg ccagctcgtg gtgccactgc
      421 gccaggaggt cgagcagcgt cgccgcccgc acgacgaaca tccccgcgtt ccagcggaac
      481 tcgcccgatg ccacgtactg cgctgcccgc acggcatccg gcttctcgac gaactcgacg
      541 gcccgcagcg ccgtggcgaa ccccggcagg gcctcgcccg cccggatgta gccgaagccc
      601 gtggcgggtg acgtcggctc gatgccgatc gtgacgagat gaccctcgcg ggccacctcc
      661 gcggcctctc ggatcacgct ctcgaaggcg gcggtgtcag ggatgacgtg gtcggccgcg
      721 aacgagccga tgacggcctg cgggtcctgg cgctcgacca cggcggcggc cagaccgatc
      781 gccgccatcg agtcgcgcgg cgacggctct gcgagcacct ggtcgccggc cagcccggga
      841 agctgagccc gcacggcatc cgcgtgcgcc gccccggtca cgacgaccac ccggtcgccg
      901 gtcagcggct cgagccggtc aaccgtcgcc tggagcagcg tgcgaccgct gccagtgagg
      961 tcgaggagga acttggggtg cgcggcgcga ctgagcggcc acagtcgcgt gccggcgcca
     1021 ccagcaggca cgacggccca gaaaccaggg atggtggaca tgggatgcga gggtacgcgg
     1081 cgcgcgctca gccccgcagc gggaagctgt cgatgactcg ccagaccccg tcgtggtcgt
     1141 ggtggtagag ctcgaccgag gtcaccggga agcggcagcg gaagtcggcc agctcgtcga
     1201 aggcccggtc gagggcgtcc tcgccgaggt ggtgggcgat cgtcacgtgc gggtggtagg
     1261 ggaagtcgag cagtcgctcg atcggcccgg agcgcacagc gcgctcgagc gcctcgcact
     1321 cgccgatccc gcgggagacc tgcacgaaca ccacgggtga gaccgggcgg aaggtccccg
     1381 tgcccgacag caccatctcg aacggcgcca ccgactcggt gaccgcgctc aggtgctcca
     1441 ggaacgacgg catctgcggc gggtggatcg cggtcggcgg aagcagggtg acgtgcggcg
     1501 ggatggctga cgccatcgtg tcgccgacgg cctcacgggc ggactggaga gtcgtcgccc
     1561 acggctcggg gatggtgacg gcaacgccat ggttcggcat ggggctggac cctactccgc
     1621 tgcgatggct gccgaggacc cggccagggc gacggcggcg acggccgccc cgacagggtg
     1681 cagcagcccc acggcatccc ggatgcgcga catcgtcgcg tcggcgacgg cggcagcccg
     1741 ctccgcaccg ctggcgagga tgcggtcgag ctcggcgggg tcgtcgagca gctcggccat
     1801 ccgcgtccgg aacggggtca cggcctcgac gacgacgtcg gcaacggcct tcttcaggtc
     1861 gccgtagccg cgcccggcga actcgtcctc gagcgccggg atcgccgtgc ccgacagcac
     1921 cgagtggatg acgagcaggt tcgacacccc gggcttggcc tcggggtcgt agcgcaccac
     1981 ggcctcggtg tcggtgacgg ccgagcggat cttcttcgcg atccgggtcg gctcgtcgct
     2041 gagcagcacc atgcccttct cggtcgaact cgaaccgctc atcttcgacg tcggctcctg
     2101 gaggtccatg atcttggccg actccttgag gatgtacgcc tcggggacag tgagcacgtc
     2161 gccgaaccgg gcgttcatcc gctcggcgag gtcgcgggtg atctccaggt gctggcgctg
     2221 gtcctcaccg acggggacga acgcggcgtc gtacatgagg atgtccgcgg ccatgagcac
     2281 cgggtaggtg aacagcccga cgttggcgtt gtgccccttg gccgtcttgt ccttgaactg
     2341 cgtcatgcgg ttcatctcac ccatggccgt ctggcacgcg agcacccacg cgagctcggg
     2401 gtgcgggccc acgtggctct ggcagaacac ggccgaggtg tgcgggtcga caccacccgc
     2461 gatgaactgg gcagcagtga cccgcgtgcg gcggcgcagc acctcggggt cggtcggcac
     2521 cgtgagcgcg tgcaggtcgg ccacgaagaa gtaggcgtcg aagtcctgct gcagcccgac
     2581 ccagttcacc agggcgccga ggtagttgcc gaggtgcagg gagtcgctcg tcggctgcat
     2641 cccggagagg atgcgcgggc gtgcggaagc gggcgaagca ggcgtggaca tgggctcatt
     2701 gtgtcagggg cagcgcccct agactcacgc gcatgacgcg agctcccggc atccacctcg
     2761 aggtgttcgg cagtgcgccg cacggtgtct ggtcggcccc cggacgggtc aacctcatcg
     2821 gcgagcacac cgactacaac gacgggctcg tgctgccgat cgccctgccg cagcgcacgt
     2881 atgccgcgtg ctcgcctcgc gatgacggcc tccttcgcgt gcactcggcc caggccggcg
     2941 caaccgtcga ggtcgatctg gcgagcgtcg ggccgggcag tccggcgggg tggacggcct
     3001 acgtcgccgg ggtgctctgg gcgctggcgc aggacggtca cgacgtgcgc ggcatggacg
     3061 tcaccgtcga cagcgaggta ccggtcggag ccggcctgtc gagctcggcg gcgctggagt
     3121 gcgcggtggg ggcggcggcc agcgacctgt tcgggctggg cctgctcgcc gacgacgcct
     3181 cgcgtgcccg cctcgccgcg gcctgcgtgc gtgccgagaa cgacgtcgcc ggcgcaccga
     3241 ccggcgggat ggaccagtcg gcatccctgc tctgccgcgc cgaccacgcc ctgctgctcg
     3301 actgccgcac cggcgccacc gagcacgtcc cgttcgtcct cggcagcggg gaggggtcgg
     3361 atgccgtggc tggccacgtc ctgctcgtca ccgacacccg ggccgagcac tcgctcaacg
     3421 acggccagta cgcgcagcgc gtcgcctcgt gcgatgccgc gtcggctgcc ctcggggtga
     3481 gctcgctgcg tgacgtcgac gttgcagggc tcgaggcgtc gctcgtcgtg ctgtccgacg
     3541 acgtcctgcg ccgccgggcc cggcacgtcg tcaccgagat cgctcgcgtg gaggcgaccg
     3601 tggccgcgct gcgcgggggt gacctgaccg aggtcgggcg cctgttcgac gagtcgcaca
     3661 cctcgctgcg cgacgactac gaggtcagct gcgccgagct cgacgtctcg gttcaggcgg
     3721 cccgctcggc cggcgccctg ggggcgcgga tgaccggtgg cgggttcggg ggctcgagca
     3781 tcgcgctcct gcccgcggat gccgtcgaca ccgccgtcgc tgcgatcgcg caggcgttcg
     3841 ccgagcgcgg gtggcgcgcc ccggcatcct tcgccgtgac cgcgggcgac tccgcccacc
     3901 gggacgcctg accc
//
LOCUS       DAOSOC010000204         3847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_23782_length_3847_cov_5.660601, whole genome shotgun sequence.
ACCESSION   DAOSOC010000204 DAOSOC010000000
VERSION     DAOSOC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23782_length_3847_cov_5.660601"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PL014_11755"
     CDS             complement(<1..612)
                     /locus_tag="PL014_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HOT57811.1"
                     /translation="MPELIFPLDSTKKFTDQKYVGHNRWHPEVPAAVHVKPGDSFRMH
                     CREWFDGAIVNDDSADDILNAPLKTVHVLSGPVAVEGAQPGDLLVVDILDVGPIPQED
                     SGPLAGQGWGYTGVFATQNGGGFLTEQFPDAYKVIWDFTGGVATSRHVPGVSFVGIVH
                     PGLMGTAPSADLLARWNAREAALIATDPDRVPPLALPPEPEHAI"
     gene            complement(676..1284)
                     /locus_tag="PL014_11760"
     CDS             complement(676..1284)
                     /locus_tag="PL014_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmiS/UreI family transporter"
                     /protein_id="HOT57812.1"
                     /translation="MGNVGLLYVGAILFINGCALLGWVRGNGAVPLNIFVGLLQVLTP
                     TYLIFTANGDPDVIFGASGLYLFGFTYLYVALNTIWGFDGTGLGYFSLFVAIAAVGYA
                     VVNVVEYQDYAFAVIWLLWAYLWWLFFQLLGRDRDGLTAWTGAVAAVEGWFTAAIPAF
                     LLLTGWWDALPNATWAVIVAVLNLGGLILARARLGSRVPAMV"
     gene            complement(1330..1620)
                     /locus_tag="PL014_11765"
     CDS             complement(1330..1620)
                     /locus_tag="PL014_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT57813.1"
                     /translation="MATYEFVCAEHGSLTITMPMAEVTATLPCPSCGEPARRRYTAPG
                     LALGDATARRLIEATSRSAHEPAVVSAPRGAPMTPRRARPIADPRTARLPAP"
     gene            1759..2574
                     /locus_tag="PL014_11770"
     CDS             1759..2574
                     /locus_tag="PL014_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOT57814.1"
                     /translation="MDSSQVAPPSAPTMLPAHRYAPERQELILRRARDQGRVEVTTLA
                     EELAVTAETVRRDLTALERRGLLRRVHGGALPIERLEVEPTLAARRTQASTQKQRIAQ
                     RAVQELPAEGTVLLDSGTTTQAIAEQFPTDRRLTVVTNSVAIAATLNTLDTIELYVLG
                     GRVRERTGAAVGQWLESALADVCVDLAFLGTNGFSVARGMTTPDQSEAMAKRAMCRAA
                     RRTIVVTDSTKAGVDHFHRFARPEEVDLLVTDTQLDDETAEQLDAAGTDVVRT"
     gene            complement(2622..3662)
                     /locus_tag="PL014_11775"
     CDS             complement(2622..3662)
                     /locus_tag="PL014_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT57815.1"
                     /translation="MSHTSTGGEARPAAAESTSPPRRWVWGVLAALVAASLITGWVLL
                     SQRGDEGATAASSPTPSASTAPSPTSATPTPAASTTVALIADFGNCGPGAQRVADMVD
                     TWDAAAVTTAGDNTYDDDPTCTPFTDSVGDFYGDWVEDPEGPRFWPALGNHDYEDKGA
                     GLEKYRAYFTYLPTEADPQQRWYDVKVDGIHLFILDNDAPDADLAAQQVWLRERLAAS
                     RAQDPAAWNVVIFHKPAFTSASHPDEVEMRPDAGWDYRGWGADVVIAGHQHVYEDVVI
                     DGMHYVTAGIATNGLERGGCDDELTDGSRLCLTGVEGAMRLDATATSLKMEYRQPSDA
                     GEVVLDAIELTR"
BASE COUNT          565 a         1299 c         1448 g          535 t
ORIGIN      
        1 aatggcgtgc tccggctcgg gaggcagggc caggggcggc acccggtcgg ggtcggtggc
       61 gatgagagcc gcctcccggg cgttccagcg ggccagcagg tccgccgacg gtgcggtgcc
      121 catgaggccg gggtggacga tgccgacgaa cgagaccccc gggacgtgcc gcgacgtcgc
      181 gacgcccccc gtgaagtccc agatgacctt gtaggcgtcc gggaactgct cggtgaggaa
      241 gccgccgccg ttctgggtgg cgaagacccc ggtgtagccc cagccctggc cggccaacgg
      301 gccgctgtcc tcctggggga tcggccccac gtcgaggatg tcgacgacga ggaggtcacc
      361 gggctgggcg ccctcgacgg cgaccgggcc ggagagcacg tggacggtct tcagcggggc
      421 gttgaggatg tcgtcggccg agtcgtcgtt gacgatcgcc ccgtcgaacc attcgcggca
      481 gtgcatccgg aaggagtccc ccggcttgac gtggacggcg gccggcacct cggggtgcca
      541 gcggttgtgg ccgacgtact tctggtcggt gaacttcttg gtcgagtcga gggggaagat
      601 cagttctggc atcggtgcgc tcctgtgcag tggcggcggc gctgggcgcc gcgggagtgg
      661 atgggaggtg cgtggtcaga ccatggccgg cacccgggac ccgaggcggg cacgggcgag
      721 gatgaggccg ccgaggttga ggacggccac gatgaccgcc cacgtggcgt tcgggagggc
      781 gtcccaccag ccggtgagga ggaggaacgc cgggatggcc gcggtgaacc agccctcgac
      841 ggcggcgacg gcaccggtcc aggcggtcag gccgtcccgg tcccgtccga gcagctggaa
      901 gaacagccac cacaggtagg cccacagcag ccagatgacg gcgaaggcgt agtcctggta
      961 ctccacgacg ttgaccacgg cgtacccgac cgccgcgatc gcgacgaaga gggagaagta
     1021 gccgaggccg gtcccgtcga atccccagat cgtgttcagc gcgacgtaga gataggtgaa
     1081 gccgaacagg tacaggcccg aggcgccgaa gatgacgtcc gggtcgccgt tcgccgtgaa
     1141 gatgaggtag gtgggcgtga ggacctggag cagccccacg aagatgttca acgggacggc
     1201 cccgttgcca cgcacccagc cgagcagcgc gcagccgttg atgaagagga tggccccgac
     1261 gtacagcagg ccgacgttac ccatggacct ccgatctgcc tgagcagccg gtgtgtgcct
     1321 gtgctcaggt cacggtgcag ggagtcgggc cgtccgcggg tcggcgatcg gtcgggcgcg
     1381 gcgcggcgtc atcggggcgc cgcggggtgc actgaccacc gctggttcgt gggcggacct
     1441 cgaggtcgcc tcgatgaggc gacgtgctgt ggcatccccg agcgcgagcc cgggtgccgt
     1501 gtaccggcgg cgagcaggct caccgcagga cgggcaggga agggtggcag tgacctcggc
     1561 catcggcatc gtgatcgtca gcgatccgtg ctcggcgcag acgaactcgt acgtggccat
     1621 caggttaccc ttcctgtgtc gtccgtcaca ccaccgtagc ggctggaagg ggggtcggcc
     1681 accggagcga gaagttgggc ctgacgggat cgcaccacga ccgcaccacg acgaccgcgc
     1741 tgaaggacaa tgggcgtcat ggactcctct caggtcgccc cgccgtcggc acccaccatg
     1801 ctcccggcgc accgctatgc ccctgagcgc caggagctca tcctgcggcg ggcccgtgac
     1861 caggggcgcg tcgaggtcac cacgctcgcc gaggagctcg cggtcaccgc cgagacggtg
     1921 cgccgggatc tcaccgcgct cgagcgtcgc ggcctgctgc gccgcgtcca cgggggcgcg
     1981 ctgcccatcg agcggctcga ggtcgagccc accctggccg cccgtcgcac ccaggcgagc
     2041 acgcagaagc agcgcatcgc ccagcgggcc gtccaggagc tacccgccga gggcaccgtg
     2101 ctgctcgact cagggacgac gacccaggcc atcgccgagc agttccccac cgaccgccgg
     2161 ctcaccgtgg tcacgaactc ggtggccatc gcggccacgc tgaacaccct ggacaccatc
     2221 gagctctacg tgctcggcgg gcgcgtgcgc gagcgcaccg gggcggcggt cggccagtgg
     2281 ctcgagtccg cgctcgccga cgtctgcgtg gacctcgcgt tcctcggcac caacggcttc
     2341 tcggtggcgc ggggcatgac gacccccgac cagtccgagg cgatggcgaa gcgggccatg
     2401 tgccgggccg cccgccgcac catcgtcgtg accgactcca cgaaggcggg cgtcgaccac
     2461 ttccaccggt tcgcccgacc cgaggaggtc gacctcctcg tcaccgacac ccagctcgac
     2521 gacgagaccg cggagcagct cgacgccgcg ggcaccgacg tggtccgcac ctgatcagga
     2581 ggctgtcgcg ccgccggtgg gcgcagcgcc ggggagcccc gtcagcgggt gagctcgatg
     2641 gcgtcgagga cgacctcacc ggcatccgag ggttggcggt actccatctt cagcgaggtc
     2701 gccgtggcgt cgaggcgcat cgcgccctcg acgccggtga ggcacaggcg gctcccgtcg
     2761 gtcagctcgt cgtcgcagcc cccgcgctcc aggccgttcg tggcgatgcc ggccgtgacg
     2821 tagtgcatcc cgtcgatgac gacgtcctcg tagacgtgct ggtggccggc gatgacgacg
     2881 tcggcgcccc agccgcggta gtcccatccg gcatccggtc gcatctcgac ctcgtccggg
     2941 tgggaggccg aggtgaacgc gggcttgtgg aagatgacga cgttccacgc ggcggggtcc
     3001 tgggcccgtg acgccgccag ccgctcgcgc agccagacct gctgggcggc caggtcggcg
     3061 tcgggtgcgt cgttgtcgag gatgaacagg tggatgccgt cgaccttgac gtcgtaccac
     3121 cgctgttggg ggtcggcctc ggtgggaagg taggtgaagt aggcccggta cttctcgagc
     3181 cccgcgccct tgtcctcgta gtcatggttg cccagggccg gccagaatcg cggcccctcg
     3241 gggtcctcga cccagtcgcc gtagaagtcg ccgacggagt cggtgaacgg ggtgcaggtg
     3301 gggtcgtcgt cgtaggtgtt gtcacccgcg gtggtgacag cagcggcatc ccacgtgtcg
     3361 accatgtcgg ccacccgctg ggctcccggg ccgcagttgc cgaagtcggc gatgagcgcc
     3421 accgtggtcg acgccgccgg ggtcggtgtc gccgaggtcg gcgacggggc ggtcgacgct
     3481 gacggcgtcg gcgacgaggc ggcggtggcg ccctcgtcgc cgcgttggga cagcaggacc
     3541 cagcccgtga tgagggacgc ggcaaccagg gcggcgagca cgccccacac ccaccgtcgt
     3601 ggtggcgacg tggactcggc ggcggcggga cgggcctcgc cgcccgtgct ggtgtggctc
     3661 atgtgactcc cctgacgtgg aggacaagtc taggcgctgg tgccggcacg acgagcagtg
     3721 gggcccccgc gcggtgcggg ggccccactg ccgttgtgct cagtgccggg tcagggcttg
     3781 ggcacgaggg agatctgcac cgcctcgacc cgcttggcct gcccggtggt gccggccgtc
     3841 gcaccgt
//
LOCUS       DAOSOC010000205         3742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_24643_length_3742_cov_8.454028, whole genome shotgun sequence.
ACCESSION   DAOSOC010000205 DAOSOC010000000
VERSION     DAOSOC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24643_length_3742_cov_8.454028"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PL014_11780"
     CDS             <1..240
                     /locus_tag="PL014_11780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT57816.1"
                     /translation="AHSTGEEFDASWNRGAPLDFRLGVGMVIAGWDQGIAGMKVGGRR
                     KLTIPPHLGYGERGAGGAIAPGETLIFVVDLMDVR"
     gene            complement(224..1339)
                     /locus_tag="PL014_11785"
     CDS             complement(224..1339)
                     /locus_tag="PL014_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HOT57817.1"
                     /translation="MMQWRAGTVVEQVRRWPGAVEYSVALDEAADPPVRALAYTAMVG
                     EPAVGERVLLNASALLRGLGTGGLAFVVARTDHLPADPETGPGHIVKARYTPMQQMLL
                     AVDEQDSPHHADLADADDLAGMPVVVADLHSALPAVLAGIRSTHPHARVAYVMTDGGA
                     LPAAFSRAVAGLADAGWLASTITVGQAFGGDHEAVTTHTGLLAARFVVGADVAVVTQG
                     PGNVGTGTRWGYSGVGCADALHAAEVLGGHGIALLRVSGADLRERHHGISHHSRTAYG
                     RALLTGADVPVTTLPDAELDALVREQLRTLVDTARARLTVVEVPADGLLEALHTSPVA
                     LRTMGRGLDSDPAPFLAAAAAGVHAAALTDSAVSGRP"
     gene            1375..2385
                     /locus_tag="PL014_11790"
     CDS             1375..2385
                     /locus_tag="PL014_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT57818.1"
                     /translation="MSNEPGVGAPSAAQVKTERLLNLVLVLLYTRRPLPKAQIRSLVP
                     QYGQSASTEAFERMFERDKDELRELGIPLVTEDIDPLHEDEPGYRIHQREYALPDIAF
                     EPDELAVLGLASRTWAQASLAGPAAAALRKLRAAGVERDVDALIGIEPRLRTTEPAFD
                     AVKDGVLRRVVLTFGYRRPEAAEVQVRTVQPWSLVSWHGRWYLNAFDLEREAPRVFRL
                     SRIVGTVKASGRAGAFEVPEDHEPQAMVRRVARPQTQPSPAVLRVRRDTGHSLRRRAR
                     TVGSVDDTWDLVDLDHGDLDAFAEELAGYGPDVVVEQPPELVDAVVERLRGALAAHGG
                     RG"
     gene            2385..3365
                     /locus_tag="PL014_11795"
     CDS             2385..3365
                     /locus_tag="PL014_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT57819.1"
                     /translation="MAGFATESATARLARLLTMVPWLVNRQGIDLRQAADDLGVSVEQ
                     LEADLNLLFLCGYGQMPDELIEADWEEGRVFVGNADTIARPLRLGVDEAVTLIVGLRA
                     LREVPGLGDRDAVERALAKLEDAAGSAAAPAARVRAEVADGGSSQVLARVRQAVTDRR
                     RVHLRYLVPARDEATERDVDPMRVVGMDGRWYLEGWCHRAGDTRMFRLDRIEELTVLD
                     VDGTPPAHARERDLSEGTFRPATDDLVVTVHLLPGATWVADYYPVESSEEVPAADGGG
                     LMVRLRTADTAWLRRLIWRLGGRGVVVDPPEVVTEVAAGAAAALTAYGEA"
     gene            3369..3683
                     /locus_tag="PL014_11800"
     CDS             3369..3683
                     /locus_tag="PL014_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57820.1"
                     /translation="MLPWWGWVLVWTVLLAASAALIGQRAWRVWGSVKGVSREAARAS
                     ALVAELEARADELRTVEPAPPAVTQSPLRMREEYRAGKAATRAARRARRAEHLPPWAR
                     VD"
BASE COUNT          487 a         1359 c         1403 g          493 t
ORIGIN      
        1 gcccactcca ccggcgagga gttcgacgcc tcgtggaacc gcggcgcgcc gctggacttc
       61 cgcctcggtg tcggcatggt catcgccggg tgggaccagg gcatcgcggg catgaaggtc
      121 ggcggccggc gcaagctgac catcccgccg cacctgggct acggcgagcg gggcgccggt
      181 ggcgccatcg cccccggcga gacgctgatc ttcgtcgtcg acctcatgga cgtccgctga
      241 ccgcggagtc ggtcaacgcg gcagcatgaa cacccgcggc ggcagccgcg aggaacgggg
      301 cggggtctga gtccagaccc cgccccatcg tgcgcagggc gaccggcgag gtgtgcagcg
      361 cctcgagcag tccgtcagcg ggcacctcga ccaccgtgag ccgggcccgc gccgtgtcca
      421 ccagggttcg cagctgctcg cgcacgagcg cgtcgagctc ggcatccggc agcgtggtca
      481 cggggacgtc cgccccggtg agcagggcgc ggccgtaggc cgtgcggctg tggtggctga
      541 tgccatggtg ccgctcgcgc aggtcggccc ccgacacccg cagcaacgcg attccgtgtc
      601 cgccgagcac ctctgcggca tgcagggcgt cggcacaccc gaccccggag tagccccacc
      661 gggtgccggt gcccacgttg cccggcccct gcgtcacgac ggcgacatcc gcaccgacga
      721 cgaaccgggc cgcgagcagg ccggtgtgcg tcgtcaccgc ctcgtggtcg ccaccgaacg
      781 cctggccgac ggtgatcgtc gacgccagcc acccggcatc cgccagtcct gcgacggctc
      841 tcgagaacgc cgccgggagg gcgccgccgt cggtcatgac gtaggcgacc cgcgcgtgcg
      901 ggtgagtgga tcggatgccg gcgagcaccg ccgggagcgc ggagtgcagg tccgcgacga
      961 ccaccggcat ccccgccagg tcgtcggcgt cggcgaggtc agcgtggtgc gggctgtcct
     1021 gctcgtcgac ggcgagcagc atctgctgca tcggggtgta gcgcgccttg acgatgtgcc
     1081 cggggccggt ctcggggtcc gccggcaggt ggtcggtgcg ggccacgacg aacgcgagcc
     1141 ctcccgtgcc cagcccacgc agtagggccg aggcgttgag caggacccgt tcgccgaccg
     1201 ccggttcacc gaccatcgcg gtgtatgcca gtgcccgaac gggcggatcg gccgcctcgt
     1261 cgagggccac ggagtactcc acggcgcccg gccaccgacg cacctgctcg accacggtcc
     1321 cggctcgcca ctgcatcacg ctcggcagcg tatccgtcag gtagcgtctg gcgggtgagc
     1381 aacgagcccg gcgtgggcgc cccctcggcg gcacaggtga agaccgagcg actgctcaac
     1441 ctcgtgctgg tgctgctcta cacccgtcgg ccgctgccga aggcgcagat ccgctcgctc
     1501 gtgccgcagt acgggcagtc ggcgtcgacc gaggccttcg agaggatgtt cgagcgcgac
     1561 aaggacgagc tgcgcgagct cggcatcccg ctggtcaccg aggacatcga cccgctgcac
     1621 gaggacgagc ccggctaccg catccaccag cgcgagtacg ccctgcccga catcgccttc
     1681 gagcccgacg agctcgctgt cctcgggttg gcgagccgca cctgggcgca ggcgagcctg
     1741 gccgggccgg ccgctgccgc cctgcgcaag ctgcgcgcgg ccggggtcga gcgcgacgtc
     1801 gacgcgctca tcggcatcga gccgcggctg cgcaccaccg aacccgcctt cgacgcggtc
     1861 aaggacggtg tgctgcgccg ggtggtgctg accttcgggt accggcgccc cgaggcggcc
     1921 gaggtgcagg tgcgcacggt gcagccgtgg tcgctcgtct cctggcacgg gcggtggtac
     1981 ctcaacgcct tcgacctcga gcgcgaggct cctcgcgtgt tccgcctctc gcggatcgtc
     2041 ggcaccgtca aggcctcggg tcgggcaggt gcattcgagg ttcccgagga ccacgagccc
     2101 caggcgatgg tccgccgggt ggcgcgtccg cagacccaac cgagcccggc cgtgctgcgc
     2161 gtgcgccgcg acaccgggca cagcctgcgc cgccgggccc gcacggtcgg gtccgtcgac
     2221 gacacgtggg acctcgtcga cctcgaccac ggcgacctcg acgccttcgc cgaggagctc
     2281 gccggctacg gccccgacgt cgtcgtcgaa caaccacccg agctcgtcga cgccgtcgtc
     2341 gagcgcctgc gcggggcgct ggctgcccac ggggggaggg gctgatggcc gggttcgcga
     2401 ccgagagtgc caccgcgcgg ctggcgcgcc tgctgacgat ggtgccgtgg ctggtcaacc
     2461 gccagggcat cgacctgcgt caggcggccg acgacctcgg ggtcagcgtc gagcagctcg
     2521 aggccgacct caacctgctc ttcctgtgcg gttacgggca gatgcccgac gagctcatcg
     2581 aggccgactg ggaggagggc cgggtcttcg tcggcaacgc cgacaccatc gcccgccccc
     2641 tgcggctcgg ggtcgacgag gcggtcaccc tcatcgtggg cctgcgggcg ctgcgcgagg
     2701 tgcccggtct gggcgaccgc gacgcggtcg agcgggccct ggccaagctc gaggatgccg
     2761 ccgggtcggc tgccgctccc gccgcgcggg tgcgggccga ggtggccgac ggcggttcgt
     2821 cgcaggtgct ggcccgggtg cgccaggccg tcaccgaccg acgccgcgtg cacctgcgct
     2881 acctcgtgcc ggcccgcgac gaggccaccg agcgcgacgt cgaccccatg cgcgtcgtcg
     2941 gcatggacgg ccgctggtac ctcgagggtt ggtgccaccg ggcgggggac acccggatgt
     3001 tccggctgga ccgcatcgag gagctcacgg tcctcgacgt cgacggcacg ccgccggcgc
     3061 acgcccgcga acgtgacctc tcggagggca ccttccgccc ggcgaccgac gacctcgtgg
     3121 tcacggttca cctgctgccg ggggcgacct gggtggccga ctactacccg gtggagtcca
     3181 gcgaggaggt cccggccgcg gacggcggcg gactcatggt gcggctgcgc accgcggaca
     3241 ccgcgtggct gcggcgactg atctggcggc tcggcgggcg tggtgtcgtc gtcgatccgc
     3301 cggaggtcgt caccgaggtg gcggccggag ccgctgccgc tctcactgcg tacggggagg
     3361 cctgagcgat gttgccctgg tggggttggg ttctggtctg gacggtgctg ctcgccgcgt
     3421 cggccgcgct gatcggtcag cgggcctggc gggtgtgggg gagcgtgaag ggcgtgagcc
     3481 gcgaggccgc gcgagccagt gccctggtcg ccgagctcga ggcgcgggcc gacgagcttc
     3541 gcaccgtgga gccggcgccc ccggccgtga cccagtctcc tctcaggatg cgcgaggagt
     3601 atcgagcagg caaggccgcg acacgggcgg cccgccgtgc gcgccgggcg gagcacctgc
     3661 cgccatgggc acgcgtagac tgacgattcg acgtgaccgt cgccacccgg cggcggacga
     3721 cagcacagga gatgcaccga tg
//
LOCUS       DAOSOC010000206         3706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_24932_length_3706_cov_8.849356, whole genome shotgun sequence.
ACCESSION   DAOSOC010000206 DAOSOC010000000
VERSION     DAOSOC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24932_length_3706_cov_8.849356"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PL014_11805"
     CDS             complement(<1..769)
                     /locus_tag="PL014_11805"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOT57821.1"
                     /translation="MSERDTQTLDIAVIGGDGIGPEVVAQGLKALDAVVGADGPKVST
                     TEYDLGARRWHATGETLPDSVLEELKGHDAILLGAIGDPTVPSGVLERGVLLPLRFAL
                     EHYVNLRPARLYPGVESPLAVERVAPNGIDFVVVREGTEGPYVGNGGSLRTGTANEIA
                     TEVSVNTRFGVERVVRDAFARAQARPRKHLTLVHKHNVLTHAGHLWRRTVDEVGAEFP
                     EVETAYQHVDAATIFLATDPGRFDVIVTDNLFGDIITD"
     gene            complement(932..1027)
                     /locus_tag="PL014_11810"
     CDS             complement(932..1027)
                     /locus_tag="PL014_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOT57822.1"
                     /translation="MNENSTLDEATLARQALQESMDLRDQGHSDD"
     gene            complement(1370..2956)
                     /gene="serA"
                     /locus_tag="PL014_11815"
     CDS             complement(1370..2956)
                     /gene="serA"
                     /locus_tag="PL014_11815"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOT57823.1"
                     /translation="MSKPVVLIAEELSPATLDALGPDFEVRHVDGADRSALLPAIADV
                     DAVLIRSATKIDAEALAAAKNLKVVARAGVGLDNVDVPAATKAGVMVVNAPTSNITSA
                     AELAVGLLLACARNIAPANEALKNGAWKRSKYGGVELLEKKVGIVGFGRIGQLVAERL
                     KGFGVEVLAYDPYVSVQRAGQLGARLVTLDELLAESDFITIHLPKTPETIGLIGTEAL
                     TKVKPSVRIINAARGGVLDEQALADAIAEGRVAGAGLDVFATEPCTDSPLFAHESVVV
                     TPHLGASTEEAQEKAGVAVAKSVRLALGGDLVPDAVNVSGGAVAEEVRPGIDLVEKLG
                     RVFTGFAGAVPVQLDVEARGEITSHDVSIWQLAALKGLFTDITEDPVTYVNAPLIAKE
                     FGCESRLLTEEVSEDFRNVTTLSGTMADGSTLSVSGTLTGPKMVQKLTGINGYDLEIP
                     LSVHMAFFSYVDRPGVIGAVGRILGDANVNIAGMQVSRDDVTGKALVALILDNEIPAD
                     VVTSVEAAIGAETVRVVDLV"
     gene            complement(3230..3703)
                     /locus_tag="PL014_11820"
     CDS             complement(3230..3703)
                     /locus_tag="PL014_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOT57824.1"
                     /translation="MESWVEGWPVWLVFGVLWLGAFTRGNATYWVGRGVRAGGGRSRF
                     ADHLERPIVRRAEGWVRRFGAPAVALGFLTVGVQTAINASAGMLRMPQRRFLPAVTVG
                     ALLWATVYTTVGFTVLDAWFGDLAWWWALAAVAVVVAIVVVSRRLERAPADSAGG"
BASE COUNT          552 a         1272 c         1337 g          545 t
ORIGIN      
        1 tgtcggtgat gatgtcgccg aagaggttgt cggtgacgat gacgtcgaag cggccggggt
       61 cggtcgccag gaagatcgtc gcggcatcca cgtgctggta cgccgtctcg acctcgggga
      121 actccgcgcc cacctcgtcg accgtgcgac gccagagatg accggcgtgg gtgagcacgt
      181 tgtgcttgtg caccagggtg aggtgcttgc gggggcgggc ctgggcgcgg gcgaaggcgt
      241 cacgcacgac gcgctcgacc ccgaagcggg tgttgacgct gacctcggtg gcgatctcgt
      301 tcgcggtgcc cgtgcgcagc gacccgccgt tgccgacgta cgggccctcg gtgccctcgc
      361 gcaccacgac gaagtcgatg ccgttcgggg ccacccgctc gacggccagc gggctctcga
      421 cgcccgggta gagacgagcg gggcgcaggt tgacgtagtg ctccagggcg aagcgcagcg
      481 ggagcagcac cccgcgctcg aggacgccgc tcggcacggt ggggtcgccg atcgccccga
      541 gcaggatcgc gtcgtggccc ttcagctcct cgagcaccga gtcgggcagg gtctcaccgg
      601 tggcgtgcca gcgtcgggca ccgaggtcgt actcggtggt cgagaccttc gggccgtccg
      661 caccgacgac ggcatcgagg gccttcaggc cctgcgcgac cacctcgggg ccgatgccgt
      721 ctccgccgat gacggcgatg tcaagggtct gggtgtcacg ctcgctcatg ccgccagcct
      781 aaggatcgat accggcatgc ggacggtcgt cccagagcgt ggaaaggccc gcgacgggaa
      841 cggcgcgccc gccgaccggc gggtccccac ggacccgggc acgacaaagg ccccggaccg
      901 gacggtcggg ggccttggcg tggtgagcga ctcagtcgtc gctgtgaccc tggtcgcgca
      961 ggtccatgga ctcctggagc gcctggcgcg ccagcgtggc ctcgtcgagg gtgctgttct
     1021 cgttcatcgg tgtcgggccc tttctcggcg cggcggacag gttcgtccct tcgagagtag
     1081 ggagatgccg gcagggcggc tccgccctct caccatccgg acgttgcagc gacgagaccc
     1141 tgatcacact gaggctcagg ctgacgcttt ccgctgccca aagacctgca tacacgtgat
     1201 tccgaaggtt gcatgacggc atccgcaccc tgcctccacg gcatccctgc gcgactcccc
     1261 cagatccgtg ccctccccga acgcagaagt tcggatggca cgcagtaatt gctgcgtgcc
     1321 atccgaactt ctgcgtggcc ggccaagcga tgggccggaa ccggctcggt cagacgaggt
     1381 cgaccacgcg caccgtctcg gcgccgatcg cggcctcgac ggaggtgacg acgtcggcgg
     1441 ggatctcgtt gtcgaggatc agggcgacca gggccttgcc ggtcacgtcg tcgcgggaga
     1501 cctgcatgcc ggcgatgttg acgttggcgt cgcccaggat cctgccgacg gcgccgatga
     1561 cgcccggccg gtcgacgtag gagaagaacg ccatgtgcac gctgagcggg atctcgaggt
     1621 cgtagccgtt gatgccggtg agcttctgca ccatcttcgg gccggtgagc gtgcccgaga
     1681 ccgagagggt cgagccgtcg gccatcgtgc cgctgagggt cgtgacgttg cggaagtcct
     1741 cggagacctc ctcggtcagc aggcgcgact cgcagccgaa ctccttggcg atgagcgggg
     1801 cgttgacgta ggtgaccggg tcctcggtga tgtcggtgaa cagacccttg agcgctgcga
     1861 gctgccagat cgacacgtcg tggctcgtga tctcgccacg ggcctcgacg tcgagctgca
     1921 ccggcacggc acccgcgaac ccggtgaaca cgcggcccag cttctcgacg aggtcgatgc
     1981 cggggcgcac ctcctcggcg accgcgccac cggagacgtt cacggcatcc ggcacgaggt
     2041 cgccgccgag ggcgaggcgc accgacttgg ccacggcgac accggccttc tcctgggcct
     2101 cctcggtcga ggcgccgagg tgcggggtga cgacgaccga ctcgtgggcg aacagggggc
     2161 tgtcggtgca gggctcggtg gcgaagacgt cgaggccggc gccggcgacc cgaccctcgg
     2221 cgatggcgtc ggcgagtgcc tgctcgtcga gcactccccc gcgggccgcg ttgatgatgc
     2281 gcacgctggg cttgaccttg gtcagcgcct cggtgccgat gaggccgatg gtctcggggg
     2341 tcttcggcag gtggatcgtg atgaagtcgc tctcggcgag cagctcgtcg agggtgacca
     2401 ggcgggcgcc cagctgtccg gcgcgctgca cggagacgta ggggtcgtac gcgaggacct
     2461 cgacgccgaa gcccttgagc cgctcggcga ccagctggcc gatgcggccg aacccgacga
     2521 tgccgacctt cttctcgagc agctcgacac cgccgtactt gctgcgcttc cacgccccgt
     2581 tcttcagtgc ttcgttggcc ggagcgatgt tgcgcgcgca ggcgagcagc aggccgacgg
     2641 cgagctcggc agcggaggtg atgttggagg tcggggcgtt cacgaccatg acgccggcct
     2701 tcgtggcggc gggcacgtcg acgttgtcca gacccacgcc ggcgcgcgcg acgaccttca
     2761 ggttcttggc tgctgccagg gcctcggcgt cgatcttggt ggccgagcgg atcagcaccg
     2821 cgtcgacgtc ggcgatggcc gggagcaggg ccgatcggtc ggctccgtcg acgtggcgca
     2881 cctcgaagtc ggggccgagt gcgtcgaggg tggccgggga cagttcttcg gcgatgagga
     2941 cgacgggctt gctcacgaaa ggaatcctgc cttcggtgct gctgcggtcg gtcatccgct
     3001 ggagatgcgg gatgccgtgg cccggacggg ccgcgcgcct caccggtgct cggcgaggcg
     3061 ctccggcacg ccgctgggcc gggagttcca tgatacgggc agcccagcat gtggactcca
     3121 ctgaccactc tgtgagagtg gacgcatgcc gccaccacgg catccagcgc ggggtcgagg
     3181 tgttaccgcc cgcgaaaccg ggcgcccacg attcaactga tccttcgact cagcccccgg
     3241 cggagtccgc aggtgcccgc tcgagtcggc gcgacacgac gacgatcgcc accaccacgg
     3301 cgaccgccgc gagggcccac caccacgcca ggtcgccgaa ccacgcgtcg agcaccgtga
     3361 acccgacggt cgtgtacacg gtggcccaga gcagggcgcc cacggtgact gccggcagga
     3421 agcgtcgctg cggcatccgg agcatgccag ccgacgcgtt gatcgccgtc tgcaccccga
     3481 cggtgaggaa gcccagcgcc acggccgggg ccccgaagcg ccggacccac ccctcggccc
     3541 ggcggacgat ggggcgctcc aggtggtcgg cgaagcgcga ccgaccaccc ccggcgcgca
     3601 ccccacggcc gacccagtag gtcgcgttcc cgcgggtgaa ggcgccgagc cagagcaccc
     3661 cgaagaccag ccacaccggc cagccctcca cccacgactc cacccc
//
LOCUS       DAOSOC010000207         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_25161_length_3682_cov_10.156603, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000207 DAOSOC010000000
VERSION     DAOSOC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25161_length_3682_cov_10.156603"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1691)
                     /locus_tag="PL014_11825"
     CDS             complement(90..1691)
                     /locus_tag="PL014_11825"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT57825.1"
                     /translation="MTRLRWLHWLDASVAIRLTFLILALFVAEISGTLIEVLPWLALI
                     TMVELVITTLDRLPVARHGVVESLVVAMLTTSAAAAGAAYGLIHTGATLLVFAPVYHA
                     GTRLGRYGALLTSAAAGGACLLALTLGRHDQDLSWSLLVWLGAAAALGILGAWNQRLA
                     AEAAAEEPDPAAREAIELIARLQDLSAQMSTGLDALASATLALDLLASDVPSTRSAVL
                     VTPDSRHLVPVALRGSTRAPWAVSDEISRVLSVGADRFTPTEVTFTDDLGDRRALVVP
                     CALGNGSTTVLVAERAATQPFTAEERRVAGEVTRRVSPTIQAGLLFGELRQVASIEER
                     DRIARDIHDGIAQELAALGYGVDALRSQAGPPGVPLRDSLDAFRQQLSSVMADLRLNI
                     TDLRVAERPGSGLGAVLGAAVQSFGSVTGMRTTVTVSERQRRLDPRVEILVHRLVMDV
                     LADAQASGARNAWVTLTASVVGPVSVEVEHDGNPRSARQTYTTPEIEGVHAHLAVRSD
                     TPGCLTVTLSVEEPTPSLGGVQGQT"
     gene            1813..2490
                     /gene="mtrA"
                     /locus_tag="PL014_11830"
     CDS             1813..2490
                     /gene="mtrA"
                     /locus_tag="PL014_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HOT57826.1"
                     /translation="MKGRVLVVDDDLALAEMLGIVLRNEGLEVHHVADGSSAIGAFRE
                     YRPDLVLLDVMLPGIDGLEVCRQIRAESGVPIVMLTARTDTVDVVVGLESGADDYVVK
                     PFKPQELIARVRARLRRGDEPEPERLTIGDLAIDVAGHSVTRGDERLSLTPLEFDLLV
                     ALARKPWQVFTREVLLEQVWGYRHAGDTRLVNVHVQRLRSKIEHDPERPEIVVTVRGV
                     GYKAGQA"
     gene            2496..>3682
                     /locus_tag="PL014_11835"
     CDS             2496..>3682
                     /locus_tag="PL014_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOT57827.1"
                     /translation="MRDALAHLGRRWRQSLQLRVVTTTMLLGLLVVSALGGVLYQQIA
                     SGLERDKIDTAQSEALALTAQAQATWDASTATSVDALDLAAFDIMRRILTAPGSNPSR
                     YVVMSRSAGNDSDIVLASLRSGSLDLSEVSPELQAAVAASPGRQQTQMSEVTVGGENL
                     PAVIIGSVVLVPNAGPYDLYFIYPLGQEVATMDLITNSFAIAGLTLTLLVGAVAWVVT
                     RQVVAPVRRASEVAQRLSAGKLNERMPSRGEDDLALLATSFNAMADSLQSQIRQLEGL
                     SAVQQRFVSDVSHELRTPLTTIRMAVDVIHDSRTGFDPSVARSAELLAGELDRFEELL
                     ADLLEISRFDAGAAALDVETVDLRSPLLRVVEATRPLADRRGSVVTLHLPDEPAEAEV
                     DERR"
BASE COUNT          532 a         1387 c         1257 g          506 t
ORIGIN      
        1 ctgggcgccc agcatcgccg ccaccccctc gcgcacgacc gcgtggtcgt cgacgagcag
       61 gaccttgagc gtcacgagcg caccctccct caggtctggc cttgcacccc gcccagtgac
      121 ggggtcggct cctcgacgct cagggtaacc gtcaggcacc ccggggtgtc cgagcgaacc
      181 gcgaggtggg cgtgaactcc ctcgatctcc ggggtcgtgt acgtctgccg cgccgagcga
      241 gggttcccgt cgtgctcgac ctcgacgctc accggcccga cgacggatgc cgtgagcgtc
      301 acccacgcgt tgcgcgcacc gctcgcctgg gcgtcggcga gcacgtccat gacgaggcgg
      361 tgcacgagga tctcgacccg ggggtcgagc cggcgctggc gctcgctcac ggtcaccgtg
      421 gtgcgcatgc cggtgacgct gccgaagctc tggacggccg ccccgagcac cgcccccagc
      481 ccgctgcccg gccgctcggc gacccgcagg tcggtgatgt tcaggcgcag gtcggccatg
      541 acactgctca actgctggcg gaacgcatcg aggctgtcgc gcaggggcac cccgggtggc
      601 ccggcctgcg accgcagggc gtcgacgccg taaccgaggg ccgcgagctc ctgcgcgatc
      661 ccgtcgtgga tgtcgcgcgc gatccggtca cgttcctcga tgctcgccac ctgccgcagc
      721 tcgccgaaca gcagcccggc ctggatcgtc ggcgagaccc ggcgggtcac ctccccggcg
      781 acccgccgct cctcggccgt gaacggctgc gtcgcggccc gctcggccac cagcacggtc
      841 gtcgacccgt tgccgagcgc acacggcacg acgagcgcac gacgatcacc gaggtcgtcg
      901 gtgaaggtca cctcggtcgg ggtgaaccgg tccgcaccga ccgacagcac ccggctgatc
      961 tcgtcggaga ccgcccacgg cgcccgcgtc gatccgcgca gcgcgaccgg cacgaggtgc
     1021 cgtgaatccg gcgtcacgag cacggcgctg cgggtcgagg gcacgtccga ggccaacagg
     1081 tcgagggcaa gggtcgcgct cgcgagggcg tccagaccgg tggacatctg ggcggagagg
     1141 tcctgcaggc gagcgatgag ctcgatggcc tcccgggcgg ccgggtccgg ctcctcggcg
     1201 gccgcctccg ctgccagccg ctggttccat gccccgagga tgccgagcgc ggctgccgcg
     1261 cccagccaga cgagcagcga ccacgagagg tcctggtcgt gtcggccgag ggtgagggcc
     1321 agcaggcagg cgccgccggc cgcggccgag gtcagcaagg ccccgtacct gcccaggcgg
     1381 gtgccggcgt ggtacaccgg ggcgaacacg agcagggtcg ctccggtgtg gatcaggccg
     1441 taggccgcac ccgccgctgc ggcggacgtc gtgagcatcg ccacgacgag gctctcgacc
     1501 acgccgtgcc gagccaccgg caaccggtcg agggtcgtga tgacgagttc gaccatcgtg
     1561 atgagggcca gccacggcag cacctcgatg agcgtgccgc tgatctcggc gacgaagagc
     1621 gcgaggatga ggaaggtgag gcgaatggcg accgaggcgt cgagccagtg gagccagcgc
     1681 agtcgggtca cctctgcagg gtagccccgg gccgtgctca ccccgcctga ccagcaccct
     1741 tgcgtgcagg gaactcccag catgctgtgc gagactgacg caacagtgtg ccccgggaaa
     1801 ggaaccgccg acgtgaaggg tcgtgtgctc gtcgtcgatg acgacctcgc gctggcggag
     1861 atgctcggca tcgtgctgcg caacgagggc ctcgaggtgc accacgtcgc cgacggctcg
     1921 tccgcgatcg gtgccttccg tgagtaccgc cccgacctcg tgctcctcga cgtcatgctt
     1981 cctggcatcg acgggctcga ggtgtgccgc cagatccggg ccgagtcggg cgtgcccatc
     2041 gtcatgctga ccgcccgcac cgacaccgtc gacgtcgtcg tcggcctgga atccggtgcc
     2101 gacgactacg tcgtcaagcc cttcaagccg caggagctca tcgcccgggt gcgggcccgc
     2161 cttcgccgtg gcgacgaacc ggagcccgag cgtctcacca tcggcgacct cgccatcgac
     2221 gtcgccggcc actcggtgac ccggggcgac gagcggctct cgctcacccc gctcgagttc
     2281 gacctcctcg tcgccctggc ccgcaagccc tggcaggtct tcacccgtga ggtgctcctc
     2341 gagcaggtct ggggctaccg gcacgccggc gacacccgac tggtcaacgt gcacgtgcag
     2401 cgcctgcgca gcaagatcga gcacgacccc gaacgccccg agatcgtcgt caccgtgcgc
     2461 ggggtcggct acaaggccgg ccaggcctga gcgcaatgcg cgatgcgctc gcccacctgg
     2521 gtcgacggtg gcggcagtcc cttcagctga gggtcgtcac gacgacgatg ctgctcggtc
     2581 tgctcgtcgt ctctgccctc ggcggggtgc tctaccagca gatcgccagc ggcctggagc
     2641 gcgacaagat cgacaccgcg cagtccgagg cgctcgccct caccgcgcag gcccaggcca
     2701 cgtgggacgc ctccaccgcg acgtcggtcg acgcgctcga cctggcggcg ttcgacatca
     2761 tgagacgcat cctcaccgcc cccgggtcca accccagccg ctacgtcgtc atgagccgct
     2821 cggccggcaa cgacagcgac atcgtgctcg cgtccctgcg cagtggctcc ctcgacctgt
     2881 ccgaggtgag ccccgagctc caggctgccg tcgcggcatc cccgggtcgc cagcagaccc
     2941 agatgagcga ggtcacggtc ggaggcgaga acctcccggc cgtcatcatc ggcagcgtcg
     3001 tcctggtacc caacgccggc ccctacgacc tgtacttcat ctacccgctg gggcaggagg
     3061 tcgcgacgat ggacctcatc acgaactcgt tcgcgatcgc cggcctcacc ctgaccctgc
     3121 tcgtcggtgc cgtcgcgtgg gtcgtcaccc ggcaggtcgt cgccccggtc cgccgcgcca
     3181 gcgaggtcgc gcagcggctg tcggccggca agctcaacga acggatgccg tcgcgcggcg
     3241 aggacgacct cgccctcctc gcgacgtcgt tcaacgccat ggccgacagc ctgcagtcgc
     3301 agatccgcca gctcgagggg ctgtccgccg tgcagcagcg cttcgtctcc gacgtctcgc
     3361 acgagctgcg cacgccgctc acgacgatcc ggatggccgt cgacgtcatc cacgactccc
     3421 gcaccggctt cgacccgtcg gtggcccgct ccgccgagct gctcgccggt gagctcgacc
     3481 ggttcgagga gctgctcgcc gacctcctcg agatcagccg cttcgacgcc ggcgccgccg
     3541 cgctcgacgt cgagaccgtc gacctgcgca gccccctgct ccgcgtcgtc gaggccaccc
     3601 gaccgttggc ggaccggcgg gggagcgtgg tcaccctcca cctgcccgac gagccggccg
     3661 aggccgaggt cgacgagcgc cg
//
LOCUS       DAOSOC010000208         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_25346_length_3661_cov_9.969218, whole genome shotgun sequence.
ACCESSION   DAOSOC010000208 DAOSOC010000000
VERSION     DAOSOC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25346_length_3661_cov_9.969218"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..796)
                     /locus_tag="PL014_11840"
     CDS             complement(131..796)
                     /locus_tag="PL014_11840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT57828.1"
                     /translation="MSPAPARGGGRRLIVLRHGETGHNAAGIWQGQLDVPLSDVGVAQ
                     ADAAGPAVAALSPSRIVTSDLARARRTADSVGRATGIRVEVDARFREIHAGAWQGLTA
                     DEVAQGWPELRAAALRGEDVRRGDDGETMGDVLVRVGEALDELLSDLPAGECAVVSTH
                     GAASRAVASWVLDLDLAVAWRLLGGLGNCHWGELREGRHGWMLHTWNASSGVPSQAGS
                     SPP"
     gene            complement(793..1173)
                     /gene="rsfS"
                     /locus_tag="PL014_11845"
     CDS             complement(793..1173)
                     /gene="rsfS"
                     /locus_tag="PL014_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT57829.1"
                     /translation="MTATDRALELARAAAAAAADKLATTITGIDVSEQLALTDVFVIV
                     SAESERQVGSIVDEVEDTLREMGSKPVRREGQREGRWVLIDFSDIVVHVQHDEERSFY
                     ELERLWKDCPSIDLGVVGTEQGGR"
     gene            complement(1170..1787)
                     /gene="nadD"
                     /locus_tag="PL014_11850"
     CDS             complement(1170..1787)
                     /gene="nadD"
                     /locus_tag="PL014_11850"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOT57830.1"
                     /translation="MRLGVMGGTFDPIHHGHLVAASEAQSLFGLDEVVFVPTGEPWQK
                     QGRDVAPAEHRYLMTVVATASNPRFTVSRVDIDRDGPTYTIDTLRDLHAQRPDDELFF
                     ITGADALAQILSWKDSDELFDLARFIGVTRPGYELSESGLPADRVDLQEVPAMAISST
                     DCRERVARGEPVWYLVPDGVVQYISKHHLYSSPEAPSPHASPGAP"
     gene            complement(1799..2032)
                     /locus_tag="PL014_11855"
     CDS             complement(1799..2032)
                     /locus_tag="PL014_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57831.1"
                     /translation="MSMTVTRFLAEEAAHRELPMPPWMFGVLGLLAFAFLLGLTWSFR
                     GTAQKYAPPTAHGEQHGAPEGDTPHWPEHPGHH"
     gene            2563..3543
                     /locus_tag="PL014_11860"
     CDS             2563..3543
                     /locus_tag="PL014_11860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT57832.1"
                     /translation="MPETVEGPRSPVELSVVVPVYNEEAVLPLLVDRLRPLADSWGVS
                     YEVLCVDDGSSDATPVLLQRLRREWPEVRVVRLRANAGHQAAISAGLARARGDWVATI
                     DADLQDPPEAIGEMLAVARAEGVDVVYGVRGDRASDTAFKRISARAFYRSIRALSAVD
                     APVDAGDFRLMSRATVDAVNALPEHHRVLRLVVPALGFPSASVSYTRAARAAGESKYP
                     LGKMIRLSVDSLTGFSIAPLRFATWLGLLGGLAALGVLVYAVIAMLLGNTLPGWTSTV
                     VIVAAVGAVQLLALGILGEYVGRMYAVLQGRPSYYVAHDSLVDSPPRSQR"
BASE COUNT          503 a         1339 c         1299 g          520 t
ORIGIN      
        1 aacccctgac cccctccatg ccatggaggt gcgctaccaa ctgcgctaca gccccttgcg
       61 ggtgttgctc ccctggtggg gggcgacggt gaagtttacc catgccaccc cacgcccgac
      121 caaatcggcc tcagggaggc gacgaaccgg cctgcgacgg caccccggac gacgcgttcc
      181 acgtgtgcag catccacccg tgccgcccct cgcgcagctc accccagtgg cagttgccga
      241 gaccgccgag caggcgccag gccaccgcga ggtcgaggtc gagcacccac gacgccaccg
      301 cccgagaggc cgccccgtgc gtcgacacca cggcgcactc ccccgccggc aggtccgaga
      361 gcagctcgtc cagggcctca ccgacccgca cgagcacgtc ccccatcgtc tcgccgtcgt
      421 ccccgcggcg cacgtcctca ccgcgcagcg cagccgcccg cagctcgggc cacccctgcg
      481 cgacctcgtc ggccgtcaac ccctgccagg cccccgcgtg gatctcccgg aatcgcgcat
      541 caacctcgac ccggatgccg gtcgcccggc ccacgctgtc ggccgtgcgt cgggcacgcg
      601 caaggtcgga ggtgacgatc cgcgacggcg acagggccgc gacagccggc cccgcggcat
      661 ccgcctgcgc gaccccgacg tcggacaacg gcacgtcgag ctgcccctgc cagataccgg
      721 ctgcgttgtg cccggtctcg ccgtggcgaa ggacgatcag gcgccgcccg ccgccccgtg
      781 caggcgctgg gctcaccgac cgccctgctc ggtgccgacg acgccgaggt cgatgctcgg
      841 gcagtccttc cagagccgct cgagctcgta gaacgagcgc tcctcgtcgt gctggacgtg
      901 cacgacgatg tcggagaagt cgatgagcac ccagcggccc tcgcgctgcc cctcacggcg
      961 aaccggcttg gagcccatct cgcgcagggt gtcttccacc tcgtcgacga tcgagccgac
     1021 ctggcgctcg gactccgcgg agacgatgac gaagacgtcg gtcagcgcga gctgctccga
     1081 gacgtcgatg ccggtgatgg tcgtggcgag cttgtcggcg gcggcggcgg cggccgccct
     1141 ggccagctcg agcgcgcggt cggtggcggt catggtgctc ccggtgaggc gtgaggtgat
     1201 ggtgcttcgg gtgatgagta gaggtggtgc ttggagatgt actgcacgac gccgtcgggc
     1261 acgaggtacc agaccggctc cccgcgagcg acccgctcac ggcagtcggt cgagctgatc
     1321 gccatggccg ggacctcctg gaggtcgacc cggtcggcgg gcaggccgga ctccgacagc
     1381 tcgtacccgg ggcgggtcac cccgatgaag cgggcgaggt cgaacagctc gtcggagtcc
     1441 ttccacgaca ggatctgcgc caacgcatcg gccccggtga tgaagaacag ctcgtcgtca
     1501 gggcgctgcg cgtgcaggtc acgcagggtg tcgatcgtgt acgtcggccc gtcgcggtcg
     1561 atgtcgacgc gcgagacggt gaagcgcggg ttggatgccg tggccaccac cgtcatgagg
     1621 tagcggtgct cggcgggggc gacgtcgcgg ccctgcttct gccacggctc acccgtgggg
     1681 acgaagacga cctcgtcgag gccgaacagt gactgggcct ccgaggcggc gacgaggtgc
     1741 ccgtggtgaa tggggtcgaa ggtgccgccc atcaccccga gccgcatacg cgccgtgctc
     1801 agtggtggcc ggggtgctcg ggccagtgcg gcgtgtcacc ctcgggggcg ccgtgctgct
     1861 cgccgtgggc ggtcgggggc gcgtacttct gggccgtgcc gcggaaggac cacgtgaggc
     1921 cgagcaggaa ggcgaacgcc aggagaccga gcacgccgaa catccacggc ggcatgggca
     1981 gctcgcggtg cgcggcctcc tcggccagga aacgggtcac ggtcatcgac atgggggtca
     2041 gcctagcgcg tccgctgtcc acgaatgtcg tccacagccc tgtgcacaag acgcgcctcc
     2101 cgcacgaggc ctgtggacga cggcgagcgc gaatgaccgg tcaccgattg gccgctcgtt
     2161 tccttgcgag gcccccacct cggcgactag aactgtcacc acgcccctgc tggacagcac
     2221 cgacgccacg gcgccggagc ggcacccggg gggcaggtcg tacggataac ggcacaccgc
     2281 tcccgccccg caagggacgg gacgtgcggg ctctcgggct cgtgcgacgc gcgcgacggc
     2341 gcgggtgccg cggccgttcg acctgagtcg aagcccgtcc tgaccccagg gcggcgccac
     2401 cgtcgcgtgg cagaccgttg cgggagaccg cacggaccga ccggctcgac acgtcggcag
     2461 cagtcgaccg gcgagggccc ccgatctcat actcgggggc cctcggccgt cgccgggccc
     2521 gccctgagcg gatcgacacc gtcggcgacc tagtcttggc ccatgccgga gactgtcgag
     2581 ggaccgcgct cgcccgtcga gctcagcgtc gtggtcccgg tgtacaacga ggaggcggtg
     2641 ctgccccttc tcgtcgaccg gctgcgaccg ctggccgact cgtggggggt cagctacgag
     2701 gtgctgtgcg tcgacgacgg ctcgagcgac gccacccccg tgctcctgca gcgacttcgg
     2761 cgcgagtggc cagaggtgcg cgtggtccgg ctgcgggcga acgccggcca ccaggcggcg
     2821 atctcggccg gtctggcgcg tgctcgtggc gactgggtcg ccaccatcga cgccgacctc
     2881 caagacccgc cggaggccat cggcgagatg ctcgccgtcg cccgcgccga gggtgtcgac
     2941 gtggtctacg gcgtgcgggg cgatcgcgcg agcgacaccg ccttcaagcg aatctccgcc
     3001 cgggcgttct accggtcgat ccgggcgttg tcggcggtgg acgccccggt ggatgccggc
     3061 gacttccggc tcatgtcccg cgccaccgtg gatgccgtga acgccctccc cgagcaccac
     3121 cgggtgctgc gcctcgtcgt cccggcgctg gggttcccga gcgcgtcggt gtcctacacc
     3181 cgggcggccc gcgccgccgg ggagtcgaag tacccgctgg gcaagatgat ccgcctctcc
     3241 gtcgacagcc tcacgggctt ctcgatcgcg ccgctgcggt tcgcgacgtg gctcgggctg
     3301 ctcggcgggc tcgcggccct gggcgtgctc gtgtacgccg tcatcgccat gctgctcggc
     3361 aacaccctgc ccggctggac gtcgaccgtg gtcatcgtcg ctgccgtcgg ggcggtgcag
     3421 ctgctggcgc tcggcatcct cggcgagtac gtcgggcgga tgtacgccgt gctgcagggc
     3481 aggccgtcgt actacgtcgc ccacgactcc ctggtggact caccacctcg ttcgcagcgc
     3541 tgagctcggc tcaggcgcgg acctgaccgt cgccctcgac gacccacttg gtcgtcgtca
     3601 gctcgggcag cgccatcggc ccgcgggcgt gcagcttctg ggtcgagatg ccgatctcgg
     3661 c
//
LOCUS       DAOSOC010000209         3645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_25490_length_3645_cov_8.361003, whole genome shotgun sequence.
ACCESSION   DAOSOC010000209 DAOSOC010000000
VERSION     DAOSOC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25490_length_3645_cov_8.361003"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PL014_11865"
     CDS             <1..654
                     /locus_tag="PL014_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT57833.1"
                     /translation="PRGTTWTERVGGWIEGTGLDAWVVQREVQDPAEYAETWARDGGH
                     HPGTADFNRMYAAWLDDFESRDVEAIGFGVVTLHRPEVERAPFVDLMDVSWPVESPMG
                     PTVLAGLRARCWLAEHDDDAVLDTSWSCAPDVHEERHSLPGAEHPSVIVLRQGGGLRR
                     HLTLTTVTAAFASVCDGDLTARAAAGAISGLLDLDADAVRDEIVAFVRDAAKDGILR"
     gene            913..1341
                     /locus_tag="PL014_11870"
     CDS             913..1341
                     /locus_tag="PL014_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57834.1"
                     /translation="MKNLPRKTWMISGALGAIALTGAVAAGGASADTRSPSPSASSLI
                     ESVPVPTDTTIVVEREASSAATSDLVVSANTPNTPNSPVTPASPTSANTPNTPNSPVS
                     ANTANTPNSPVSPVSPASPVSANTPNTPNSPASPVSAPSA"
     gene            1352..2587
                     /locus_tag="PL014_11875"
     CDS             1352..2587
                     /locus_tag="PL014_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT57835.1"
                     /translation="MTGTASGLPGLPATTSPRESREADVLELLTGPGAREPLSLAVAT
                     VQAELADHEVHSVQHRPGDGVTVGYRVWLRTASGDLVEDYVLLSSTAGRDVPDDAAHV
                     VSMQGPSGRLLGWRHPHDPALPGLEVACDPVALEHVVPGSGVVTSIELLGYRPLRRAV
                     VRAVRDGRTAYVKVLRPAAGRGGAPDVLHRLAVLAEAGLPVPAVLAAQSDGLVVLEEV
                     VGTPLVEAIGQDDASGLELDQLVALLDALPASVLDLPRRPAWSDRAADYADAVAAAGI
                     HAHRALAVGREVVARSRTVDLGPVVATHGDFHEGQLTVTRVGEGWRPSGLLDVDTVGP
                     GHRVDDLACLVAHAIALGPAGEAVARRWEQAAQVVVDREALAVRTAGALLSLAAGAVH
                     EAHLAMSTADLLDAAEARL"
     gene            2662..3252
                     /locus_tag="PL014_11880"
     CDS             2662..3252
                     /locus_tag="PL014_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT57836.1"
                     /translation="MPPRAPALSPEDRRAALVDVTLPLLREHGPSVTTRQVAEAAGIA
                     EGTVFRAFGSKDELVQACVAAVFDSAPAVAELRAIDPSLTLDERLTSGVAILLRHVES
                     IVGLISVLHHAGSPAGGPVAHDAKRHQRPSDPEVDAAFLDLIGDDAAALRKPASDVVA
                     LLQLLTLSSVHPLMSTGRLDAADIVDVVLDGTRKKA"
     gene            3252..>3645
                     /locus_tag="PL014_11885"
     CDS             3252..>3645
                     /locus_tag="PL014_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOT57837.1"
                     /translation="MLYRLVRTHLTPYAASLLVLLALQFVATIASLYLPSLNGRIIDE
                     GVAVGDTGFIMRAGAIMLVVSLVQIAATIAATRIGAQSAASLGRDVRARVFRTVGAFS
                     AQELSRFGAPTLISRSTNDVTQVQTVVYM"
BASE COUNT          473 a         1387 c         1265 g          520 t
ORIGIN      
        1 ccgcgcggca cgacgtggac cgagcgggtc ggcggctgga tcgagggcac cggcctggac
       61 gcctgggtcg tgcaacggga ggtgcaggac ccggcggagt acgccgagac gtgggcgcgc
      121 gacggggggc accacccggg caccgccgac ttcaacagga tgtatgccgc gtggctcgac
      181 gacttcgagt cccgcgacgt cgaggcgatc gggttcggcg tcgtgacgct gcaccgtcct
      241 gaggtcgagc gggctccctt cgtcgacctc atggacgtgt cgtggcccgt cgagtcgccg
      301 atgggtccga cggtgctggc cgggctccga gcgcgctgct ggctcgctga gcacgacgac
      361 gacgccgtgc tcgacacctc gtggtcgtgt gctcccgacg tgcacgagga acggcacagc
      421 ctgccgggcg ccgagcaccc gagcgtcatc gtgctgcgac agggcggggg gttgcggcgc
      481 cacctgacgc tgacgaccgt gaccgctgcc ttcgcctcgg tgtgtgacgg cgacctgacc
      541 gcgcgggctg cggcgggggc gatctcgggg ctgctggacc tcgacgcgga tgccgtgcgt
      601 gacgagatcg tggccttcgt ccgggacgcg gccaaggacg gcatcctgcg ctgaccaatc
      661 cggtcggcgc tcggtgacga accaccatcg acgggccgga ctggccggcc tgaccgatgg
      721 tgaggccggc acgggcctga acccacgacc tggcaggggg gtcgtggggc agggccgcca
      781 gtcccccgtc atcggtcgtg gggcggacat gaggacgccc tcatgttcgc ctcacgacca
      841 cctcatcaac gtcctcgagg ctggtgcaac agcaggcggc accccgtcgc cccctttcca
      901 ggaggacctg agatgaagaa cctgccccgc aagacgtgga tgatcagtgg cgcgctcggc
      961 gccatcgccc tcaccggtgc cgttgcggcc ggcggcgcat cggcggacac ccgcagcccc
     1021 tccccgagcg cctccagcct catcgagtcg gtgcccgtgc ccaccgacac gaccatcgtg
     1081 gtggagcgcg aggcgtcgag cgcggcgacg agcgacctcg tggtctcggc gaacacgccc
     1141 aacaccccca actcgcccgt gaccccggcc agcccgacgt cggcgaacac gcccaacacc
     1201 cccaactcgc cggtgtcggc caacacggcg aacaccccca actcgccggt gtccccggtc
     1261 tccccggcca gcccggtgtc ggcgaacacg cccaacaccc ccaacagccc ggcatccccg
     1321 gtctccgccc ccagcgcgtg accctcagtt gatgaccggc acggcatccg gcctcccggg
     1381 actcccggcg accacgtcgc cgcgggagtc ccgggaggcc gacgtgctcg agctgctcac
     1441 cggccccggc gcgagggaac cgctctcgct cgccgtcgcg acggtgcagg ccgagctggc
     1501 ggaccacgag gtgcactccg tccagcaccg tccgggcgat ggtgtcaccg tcggctaccg
     1561 ggtgtggctg cgcaccgcct cgggcgatct cgtcgaggac tacgtgctcc tcagctccac
     1621 cgccgggcgt gacgtgcccg atgacgccgc ccacgtggtg agcatgcagg ggccgtctgg
     1681 gcggctgctc gggtggcggc atccgcacga ccccgcactg ccggggctcg aggtcgcgtg
     1741 cgaccccgtg gccctggagc acgtcgtgcc ggggagcggg gtcgtgacga gcatcgagct
     1801 gctggggtac cggcccctgc gtcgggccgt ggtccgtgcg gtgcgtgacg ggcggacggc
     1861 gtacgtcaag gtgctgcgcc ccgcagcggg gcgtggtggc gccccggacg tccttcaccg
     1921 gttggcggtc ctcgccgagg ccgggctgcc cgtgcccgcg gtgctcgcgg cccagagcga
     1981 cgggctcgtc gtcctggagg aggtcgtcgg caccccgctc gtcgaggcca tcggccagga
     2041 cgacgcgagc ggcctcgagc tcgaccagct cgtcgcgctg ctcgacgccc tgccggcgtc
     2101 ggttctcgac cttcctcggc gcccggcgtg gtcggaccgg gctgccgact atgcggatgc
     2161 cgtggccgcc gccggaatcc acgcccaccg tgcgctcgcc gtcggtcgcg aggtcgtggc
     2221 ccggtcccgg acggtggacc tcgggccggt cgtcgcgacc cacggtgact tccacgaggg
     2281 gcagctgacg gtcacccggg tgggggaggg ctggcgcccg agcggcctgc tcgacgtcga
     2341 caccgtcggg cccggccacc gggtcgacga cctggcctgc ctcgtggcgc acgcgatcgc
     2401 gctgggccct gctggagagg cggtggcgcg tcgctgggag caggcggctc aggtcgtcgt
     2461 cgaccgcgag gcgctggccg tgcgcacggc gggcgcgctc ctgtcgctcg cagcgggtgc
     2521 ggtgcacgag gcgcacctgg cgatgtcgac ggctgacctg ctcgatgccg ccgaggcgcg
     2581 cctctaggtc tgctgacccc cacgaacacc cgagcgtcaa acctgttggt gagtgagtgc
     2641 tcactcactt atagtgtccg ggtgccaccc cgtgccccag ccctcagccc cgaagaccgc
     2701 cgcgccgccc tcgtcgacgt gacccttccg ctcctgcgcg agcacggccc gtccgtgacg
     2761 acccgccagg tcgccgaggc cgccggcatc gccgagggca ccgtcttccg cgcgttcggc
     2821 agcaaggacg aactcgtcca ggcctgcgtc gccgcggtgt tcgactccgc gccggccgtc
     2881 gccgaactgc gcgccatcga cccctcgctg accctcgacg agcggctcac ctcgggggtc
     2941 gccatcctgc tgcggcacgt cgaaagcatc gtgggcctca tctcggtgct ccaccacgcc
     3001 ggttcgccgg ccggtggccc ggtcgcccat gacgcgaagc gacaccagcg accgtcggac
     3061 cctgaggtcg acgccgcctt cctcgacctc atcggtgacg atgccgcggc cctgcgcaaa
     3121 ccggcatccg acgtcgtcgc cctgctccag ctcctcacgc tctcgagcgt gcacccgctc
     3181 atgtcgaccg gccgcctcga cgcggccgac atcgtcgacg tcgtcctcga cggcacccgg
     3241 aagaaggcct gatgctctac cgactcgtgc gcacgcacct caccccgtac gcggcctccc
     3301 tgctggtgct gctcgccctc cagttcgtcg cgacgatcgc gtcgttgtac ctgcccagcc
     3361 tcaacggccg gatcatcgac gagggcgtcg cggtcggcga caccggcttc atcatgcgcg
     3421 cgggcgcgat catgctcgtg gtctccctcg tgcagatcgc ggccacgatc gcggcgaccc
     3481 gcatcggtgc ccagtccgcg gcatccctgg gccgtgacgt ccgcgcccga gtcttccgca
     3541 ccgtcggtgc cttctccgcg caggagctct cccgcttcgg cgcgcccacc ctcatcagcc
     3601 gcagcaccaa cgacgtgacc caggtgcaga ccgtcgtcta catga
//
LOCUS       DAOSOC010000210         3629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_25648_length_3629_cov_9.193621, whole genome shotgun sequence.
ACCESSION   DAOSOC010000210 DAOSOC010000000
VERSION     DAOSOC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25648_length_3629_cov_9.193621"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PL014_11890"
     CDS             complement(<1..947)
                     /locus_tag="PL014_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT57838.1"
                     /translation="MSAPAIEAVDLVKTFGDFTAVDGVSFSVPAGTVFSMLGPNGAGK
                     TTTVRMMTTLSIPTSGTAHVAGFDVAKNPDAVRHRMGLTAQSATVDELLTGRENLRLI
                     GDLYGLERSLVKRSTEELLERFSLTEAGNKVVKDYSGGMRRRLDLAASLIATPPVLFL
                     DEPTTGLDPRSRVELWGVLRDLVRDGTTLFLTTQYLDEADQLADRIVVVDHGRIIAEG
                     TPLELKDQSGKASLVVTVSRAPEVDAAAELVRAYVGEIHVDHDSRRISAPAEGVSALT
                     RIAARLEEAAIELDDIGLQRPSLDDVFLSLTGHRAETET"
     gene            complement(1053..2408)
                     /locus_tag="PL014_11895"
     CDS             complement(1053..2408)
                     /locus_tag="PL014_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOT57839.1"
                     /translation="MTSASARVLCVDVGSTFTKAVLVDTTTGAVLGTASHPTTVSTDV
                     MDGVDAVRADLAGSGEPTEVLACSSAGGGLRLAVVGYEREVTAEAGQRVGLSAGAKVV
                     HVASGAMTGDDVKALRAASPDLVLLVGGTDGGNARVLLHNAERLAKARLSAPVVVAGN
                     TDAADEVAALLTSTGRRFVVADNVLPRIGVVAPESARAAIREAFLTHVIGGKGLSRGR
                     GFAELVQAPTPDAVLRGVEVLAEVVGGDVLVVDVGGATTDVYSVITPQGEDASIQREV
                     VGTLWHARTVEADLGMRWSAQGVVEACARERRALDGATAEYAARVAADTAHLASSPQE
                     WLAEEEIASAAAVLAVRRHGRPGHPSERPRPLAEVTVLVGSGGVLRHAPGGVGDRVLE
                     QVLSDHGGGWRVPVAARAGVDTAYLLFAVGLLADHHPEAAAALAAQVVAPPVATTRHP
                     D"
     gene            complement(2416..3594)
                     /locus_tag="PL014_11900"
     CDS             complement(2416..3594)
                     /locus_tag="PL014_11900"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOT57840.1"
                     /translation="MTFEHLGFSSRAIHAGQEPDPTTGAVVPPIHQVSTYKQDGVGGL
                     RGGYEYSRSANPTRTALEECIAALEGGERGFAFASGLAGEDTVLRALLAPGDHVVVPS
                     DAYGGTYRLFNKVARPWGVEHSIATVADVESVRAAIRPQTRMVWVETPTNPLLGIADI
                     EAIAAVAHEAGALLVVDNTFASPYLQNPIALGADVVVHSTTKYAGGHSDVVGGAVVVA
                     PGATTRDGESVSERIAFHQNSMGAVAGPFDAWLVLRGLKTLAVRMERHCDNAEKVVAF
                     LQGHDGVTAVHYPGLPDHPNHDVAARQMRRFGGMVAFRVKGGEDVAAKVCAETQLWIL
                     GESLGGVESLIEHPGRMTHASVAGTELEVPGDLIRLSVGIEDAEDLIADLGQALDRLT
                     "
BASE COUNT          505 a         1276 c         1355 g          493 t
ORIGIN      
        1 tcggtctcgg tctcggcgcg gtggccggtc aggctgagga agacgtcgtc gaggctgggc
       61 cgctggagcc cgatgtcgtc gagctcgatg gcagcctcct ccaggcgtgc cgcgatgcgg
      121 gtcagcgcgg acaccccctc ggccggggcg gagatgcggc gcgagtcgtg gtcgacgtgg
      181 atctcaccga cgtacgcccg caccagctca gccgcggcgt cgacctcggg ggcccggctc
      241 acggtgacga cgaggctggc cttgccggac tggtccttca gctccagagg ggtgccctcg
      301 gcgatgatcc ggccgtggtc gacgacgacg atgcggtcgg ccagctggtc ggcctcgtcg
      361 aggtactggg tggtgaggaa cagggtcgtc ccgtcgcgca cgaggtcgcg cagcacaccc
      421 cagagctcga cccggctgcg cgggtcgagg ccggtcgtcg gctcgtcgag gaagagcacc
      481 ggaggggtgg cgatgaggct ggcggcgagg tcgagccggc gtcgcatgcc gcccgagtag
      541 tccttgacga ccttgttgcc agcctcggtc agcgagaacc gctcgagcag ctcttcggtc
      601 gagcgcttca cgagcgagcg ctccagcccg tacaggtcgc cgatgaggcg caggttctcg
      661 cgcccggtga gcagttcgtc gaccgtggcc gactgggccg tcaggcccat ccggtgccgc
      721 acggcatccg ggttcttggc gacgtcgaag ccggcgacgt gtgccgtgcc gctggtggga
      781 atcgacagcg tggtcatcat gcgcacggtc gtcgtcttgc ccgcgccatt cgggccgagc
      841 atggagaaaa ccgtgcccgc cggcacggag aagctcacgc cgtcgacggc ggtgaagtca
      901 ccaaaggtct tgacgaggtc aacggcctcg atcgcggggg cgctcaccgg tgacgcaccg
      961 ggcgaagagt ggtggaagtc atgggacggg aggctacgtg agcccaccga cacccgtcat
     1021 ccggttttgc cggacgggcc ccaatagtcc tcctaatccg gatgccgtgt ggtcgccacc
     1081 gggggcgcca cgacctgcgc cgcgagggcc gcggccgcct cggggtggtg gtcggcgagc
     1141 aaccccaccg cgaacagcag gtaggccgtg tcgacgcccg cacgcgctgc cacaggcacc
     1201 cgccaccccc caccgtggtc gctcagcacc tgctccagca cccggtcccc gactcccccg
     1261 ggcgcgtgcc gcagcacccc accggacccg acgagcacgg tgacctccgc cagcggacgg
     1321 ggccgctccg acgggtggcc cggccggccg tggcgccgaa ccgccagcac cgcggcagcg
     1381 ctcgcgatct cctcctcggc cagccactcc tgcggcgagc tcgcgaggtg agcggtgtcg
     1441 gcggccacgc gcgcggcgta ctcggccgtt gcgccatcga gcgcccggcg ttcacgagcg
     1501 caggcctcga cgacaccctg cgccgaccag cgcatgccga ggtcggcctc gacggtgcgg
     1561 gcgtgccaca acgtgccgac gacctcgcgc tggatgctgg cgtcctcgcc ctgcggggtg
     1621 atcaccgagt acacgtcggt cgtcgcaccg ccgacgtcga ccacgaggac gtctcccccg
     1681 acgacctcgg cgagcacctc gacgccgcgc aacacggcat ccggcgtcgg tgcctggacg
     1741 agctcggcga aaccgcgacc acgggacagg cccttgccgc cgatgacgtg ggtgaggaag
     1801 gcctcgcgga tggcggcgcg cgccgactcc ggcgccacga ccccgatgcg cggcaacacg
     1861 ttgtctgcca cgacgaacct ccgccccgtc gaggtgagca gcgccgcgac ctcgtccgcg
     1921 gcatccgtgt tgcccgcgac gacgaccggc gccgagagtc gggccttggc cagccgctcc
     1981 gcgttgtgca gcagcacccg ggcgttgcct ccgtcggtgc cgccgacgag gaggacgaga
     2041 tcgggggatg ccgcccgcag cgccttgacg tcgtcgcccg tcatcgcgcc gctggcgacg
     2101 tggacgacct tggcacccgc ggagagcccg acccgctgcc cggcctcggc ggtcacctcc
     2161 cgctcgtacc ccacgacggc cagccgcagg ccgccccccg cgctggagca ggcgagcacc
     2221 tcggtgggct cgccggaccc cgccaagtcg gcacgtacgg catccacccc gtccatgacg
     2281 tcggtgctga cggtggtcgg gtggctggcg gtgccgagga cggcacccgt cgtggtgtcg
     2341 acgagcaccg ccttggtgaa ggtcgacccg acgtcgacgc acaggacgcg cgcgctggcg
     2401 gaggtcacag cgggctcagg tgaggcggtc gagggcctgg ccgaggtcgg cgatgaggtc
     2461 ctcggcgtcc tcgatgccga cggagagccg gatcaggtca cccggcacct cgagctcggt
     2521 gccggcgacc gaggcgtggg tcatccggcc cgggtgctcg atgagcgact cgacgccacc
     2581 gagggactcg ccgaggatcc acagctgggt ctcggcgcag accttggccg cgacgtcctc
     2641 gccacccttg acgcggaagg cgaccatgcc gccgaagcgg cgcatctggc gtgcagcgac
     2701 gtcgtggttg gggtggtcgg gcaggcccgg gtagtgcacg gcggtgacgc cgtcgtggcc
     2761 ctggaggaac gccacgacct tctcggcgtt gtcgcagtgc cgctccatgc gcacggcgag
     2821 ggtcttcagg ccgcgcagca cgagccacgc gtcgaagggc ccggcgacgg cgcccatcga
     2881 gttctggtgg aaggcgatcc gctcgctgac cgactcaccg tcgcgggtcg tggcgcccgg
     2941 ggcgacgacg acggcgccac cgacgacgtc gctgtggccg ccggcgtact tggtcgtgga
     3001 gtgcacgacg acgtcggcgc ccagggcgat cgggttctgc aggtacgggg aggcgaaggt
     3061 gttgtcgacg acgagcagtg caccggcctc gtgggcgacc gccgcgatgg cctcgatgtc
     3121 ggcgatgccg agcagcgggt tggtcggcgt ctcgacccac accatgcggg tctgcgggcg
     3181 gatggcggcg cgcaccgact cgacgtcggc gacggtcgcg atcgagtgct cgacacccca
     3241 cgggcgagcg accttgttga acaggcggta ggtgccgccg tacgcgtcgg acggcacgac
     3301 gacgtggtcg ccgggggcga ggagtgcgcg cagcacggtg tcctcaccgg cgaggccgct
     3361 ggcgaaggcg aacccgcgct caccaccttc gagggcggcg atgcactcct cgagggcggt
     3421 gcgggtcggg ttggcggagc ggctgtactc gtagccgccg cgcaggccgc cgacgccgtc
     3481 ctgcttgtag gtgctcacct ggtggatggg agggacgacg gccccggtcg tggggtcggg
     3541 ctcctgcccg gcatggatgg cgcgcgagga gaagccgagg tgctcgaagg tcatggcgcc
     3601 cagcgtatgc cgttgcgtgg gcttccccg
//
LOCUS       DAOSOC010000211         3579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_26140_length_3579_cov_8.425653, whole genome shotgun sequence.
ACCESSION   DAOSOC010000211 DAOSOC010000000
VERSION     DAOSOC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26140_length_3579_cov_8.425653"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PL014_11905"
     CDS             complement(<1..838)
                     /locus_tag="PL014_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HOT57841.1"
                     /translation="MTATTLDGKAILETIKGELRARVAALAEQGVVPGLGTVLVGEDP
                     GSQWYVGAKHRDCAEIGIRSIRRDLPSGTTQDEVLEVVRGLNEDPECTGFIVQQPTGL
                     DENAILSAVDPTKDVDGLHPMNLGWLVLGETAPLPCTPVGCIELLRRYDVPIAGAKVV
                     VVGRGLTVGRPLGLILTRRSENATVTLCHTGTRDLAAEVRQADIVVAAAGVPGIITGD
                     MVKPGAAVLDVGVSRIEDVESGKSIVAGDVHPSVWEVAGFVSPNPGGVGPMTRAMLLS
                     NVV"
     gene            complement(851..2272)
                     /locus_tag="PL014_11910"
     CDS             complement(851..2272)
                     /locus_tag="PL014_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOT57842.1"
                     /translation="MTTSSRPLHGLLEADPVVATAGVPLLADALSDQAVRVSRTQWQP
                     PMAGTEADLARVMGDPRRAAANALALERMTSATATLVDVRPASEVVGLERGTFLHAGP
                     PITWERASGPMRGALIGAVLFEGLADTAEDAEQRLATGEFTLEPCHHRGGVGPMAGVV
                     SPSMWLYVLRDEVHDATSWCSLNEGLGKVLRYGAYGPEVIDRLHWMTRVLGPILSQAV
                     RSTGPIDIRSIITQMLQMGDEGHNRNRAGSLMLLRELLPGMITADAASTDVAEAVRFS
                     GANEHFFLNLGMPACKLATMAASGIPGSSVVTVMARNGTDFGIQVSGTGNDWFTGPAN
                     TPEGLFLGTFGPDDANPDIGDSAITETGGIGGFAMAAAPAIVKFVGGDVPFALRATRT
                     MYEITVGEHPAYQVPILGFRGTPTGIDAALVARTGILPQINTGMAGRVAGTGQVGAGL
                     VTPPAECFPAALAALAARVPEGP"
     gene            complement(2269..>3579)
                     /locus_tag="PL014_11915"
     CDS             complement(2269..>3579)
                     /locus_tag="PL014_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HOT57843.1"
                     /translation="IALRTDTPDAVAAGLAAVADALAALRATSDSGGGDDTVAPRTLG
                     SAAARVDATLAVISVPGQHAVTEAFDAITSGLSVMLFSDNVSVEDEVHLKDAAAAADV
                     LVMGPDCGTAHIGGVALGFANVVRPGSVGIVAASGTGAQQVMCLLDAAGVGISHCLGV
                     GGRDLKAAVGGRSTRQALRALAADPATDQVIVVSKPPDAGVLADLEALADELGLQVHW
                     AVLGPGRPDLTASIESFLVAQGVPVPTWPTWAAAGGGDDPLLSRGAALRGLFCGGTLA
                     DEAITVAEGGLGGIHSNIPHDPALALGADLRHDGHVVIDFGDDGLTRGRAHPMIDPTL
                     RQERIAAEALDPTCGVLLLDLVLGHGAHPDPADELADAVRTARATALASGRALPVVVS
                     LTGTDGDPQHRSRCAEVLAAAGADVYLSNAAATRAALSLLGSAS"
BASE COUNT          490 a         1288 c         1329 g          472 t
ORIGIN      
        1 ggacgacgtt ggacagcagc atggcccggg tcatcggccc gacacctccg gggttggggc
       61 tgacgaaccc ggcgacctcc cacacgctcg ggtgcacgtc accggcgacg atcgacttgc
      121 cgctctcgac gtcctcgatg cgcgagaccc cgacgtcgag caccgcagcc cctggcttga
      181 ccatgtctcc ggtgatgatg ccgggcaccc ccgcggccgc gacgacgatg tcggcctggc
      241 gcacctcggc ggccaggtca cgggtcccgg tgtggcacag cgtgaccgtg gcgttctccg
      301 agcggcgggt gaggatgagc ccgaggggtc ggcccacggt gaggccgcgc ccgacgacga
      361 cgaccttggc accggcgatc ggtacgtcgt agcggcgcag cagctcgatg cacccgaccg
      421 gcgtgcacgg cagcggcgcc gtctcgccga gcacgagcca cccgaggttc atcgggtgca
      481 ggccgtcgac gtccttcgtg gggtcgacgg ccgacaggat ggcgttctcg tcgagcccgg
      541 tcggctgctg gacgatgaag ccggtgcact ccgggtcctc gttgagcccg cgcacgacct
      601 cgagcacctc gtcctgggtc gtcccggagg gcaggtcgcg gcggatgctg cggatgccga
      661 tctcggcgca gtcgcggtgc ttggccccga cgtaccactg gctgcccggg tcctcaccga
      721 cgaggacggt tccgagcccg gggacgacgc cctgttcggc aagggcggcg acgcgcgccc
      781 ggagctctcc cttgatcgtc tcgaggatgg ccttgccgtc aagcgtcgtc gcggtcacgg
      841 gccaatcctt tcacggccct tcaggcaccc gtgccgcaag agcggcaagt gccgccggga
      901 aacactccgc aggcggggtc accaacccgg ccccgacctg cccggtgccc gcgacccgtc
      961 cggccatccc cgtgttgatc tggggaagga tgccggtgcg tgccacgagc gcggcgtcga
     1021 tcccggtcgg ggtgccgcgg aacccgagga tcggcacctg gtaggccggg tgctccccca
     1081 ccgtgatctc gtacatcgtg cgcgtcgccc gcagcgcgaa cggcacgtca ccgccgacga
     1141 acttgacgat cgccggggcc gcggccatgg cgaacccgcc gatgccgccg gtctcggtga
     1201 tcgccgagtc gccgatgtcg gggttcgcgt cgtccggccc gaaggtgccg aggaacagcc
     1261 cttccggggt gttcgcgggc ccggtgaacc agtcgttgcc cgtccccgac acctggatgc
     1321 cgaagtcggt gccgttgcgc gccatgacgg tcacgacgct cgacccgggg atcccggagg
     1381 ccgccatcgt cgcgagcttg caggccggca tcccgaggtt gaggaagaag tgctcgttcg
     1441 ccccggagaa ccgcaccgcc tccgcgacgt cggtcgacgc ggcatccgcc gtgatcatcc
     1501 ccgggagcag ttcacgcagg agcatgagcg acccggcgcg gttgcggttg tgcccctcgt
     1561 cgcccatctg gagcatctgg gtgatgatcg accggatgtc gatgggcccg gtcgagcgca
     1621 ccgcctgcga caggatcggc ccgagcaccc gcgtcatcca gtgcagccgg tcgatgacct
     1681 ccggcccgta ggcgccgtag cgcagcacct tgccgagccc ctcgttgagc gagcaccacg
     1741 acgtcgcgtc gtgcacctcg tcgcgcagca cgtacagcca catcgacggg ctgacgacgc
     1801 ccgccatcgg gccgacccca ccgcggtggt ggcacggctc gagggtgaac tcccccgtgg
     1861 cgagacgttg ttcggcgtcc tccgcggtgt ccgccaaccc ttcgaacagc accgccccga
     1921 tgagggcgcc gcgcatcggc cccgacgcgc gctcccacgt gatcggcggg ccggcgtgca
     1981 ggaaggtgcc ccgctccaac cccacgacct cggatgccgg tcgcacgtcg accagggtcg
     2041 ccgtcgccga ggtcatccgc tccagtgcga gcgcgttggc cgctgcccgg cgcgggtcac
     2101 ccatgacgcg ggcgaggtct gcctcggtgc cggccatcgg cggctgccac tgggtgcggc
     2161 tcaccctgac ggcctggtcg ctgagggcgt cggcgagcaa cggcaccccg gcggtggcga
     2221 cgacggggtc agcctcgagc aggccgtgca ggggtcggga ggatgtggtc atgaggcgct
     2281 ccccagcagc gacagggcag cgcgggtggc cgcggcattg gacaggtaca cgtcggctcc
     2341 ggcggcggcc agcacctcgg cacacctcga gcggtgctgc gggtcgccgt cggttccggt
     2401 gagcgacacc acgaccggca gggcgcgccc ggaggcgagg gcggtcgccc gagccgtgcg
     2461 gacggcatcc gcgagctcgt ccgcggggtc ggggtgtgcg ccgtgcccga ggacgaggtc
     2521 gagcagcagc acgccgcagg tggggtcgag cgcctcggcg gcgatccgct cctgacgcag
     2581 ggtcgggtcg atcatcgggt gggcgcggcc gcgggtcagc ccgtcgtcac cgaagtcgat
     2641 gacgacgtgg ccgtcgtggc gcaggtcggc gccgagcgcc agggccgggt cgtgcgggat
     2701 gttgctgtgg atgccgccca gcccaccctc ggcgaccgtg atcgcctcgt cggcgagggt
     2761 gcctccgcag aacaggccac gcagggcggc gccccgggac aggagcgggt cgtcgccacc
     2821 gccggcggca gcccacgttg gccacgtcgg caccggcacc ccctgggcga cgaggaagga
     2881 ctcgatggat gccgtgaggt cggggcggcc cggccccagc accgcccagt gcacctgcag
     2941 ccccagctcg tcggcgaggg cttcgaggtc ggcgaggacc cctgcgtccg gtggcttgga
     3001 gacgacgatg acctggtcgg tcgccgggtc ggcggccagt gcacgcagcg cctgccgggt
     3061 cgagcgccca ccgacggccg ccttgaggtc acggccgccg acgccgaggc agtgcgagat
     3121 gccgacaccg gccgcgtcga gcaggcacat gacctgctgg gcgccggtcc ccgacgcggc
     3181 gacgatgccg accgagccgg ggcgcacgac gttggcgaac ccgagggcga ccccgccgat
     3241 gtgggcggtg ccgcagtcgg gccccatgac gagcacgtca gcggcggcgg cggcatcctt
     3301 gaggtgcacc tcgtcctcga cggacacgtt gtcggagaag agcatcaccg acaggccgga
     3361 ggtgatggcg tcgaaggcct cggtgacggc gtgctgcccc ggcaccgaga tgacggccag
     3421 cgtggcgtcc accctggccg cggccgagcc gagggtgcgc ggggcgaccg tgtcgtcgcc
     3481 accaccggag tcgctcgtcg cccgcaatgc cgcgagcgcg tcggcgacgg ccgcgagacc
     3541 cgccgccacg gcatccgggg tgtcggtgcg cagggcgat
//
LOCUS       DAOSOC010000212         3574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_26198_length_3574_cov_9.473714, whole genome shotgun sequence.
ACCESSION   DAOSOC010000212 DAOSOC010000000
VERSION     DAOSOC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26198_length_3574_cov_9.473714"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PL014_11920"
     CDS             <1..1098
                     /locus_tag="PL014_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT57844.1"
                     /translation="DPGLEEVARSLGRTPWQTFREVTLRQVRPSAAAGGLLVALYTLS
                     DFGAVSILRYDVFTRVIYTAYRSSFDRSTAAVLSLLLVALTVAISVGESRVRRRDVAR
                     IGAGVARQPEPLRLGPLRLPMAGGAGVVVVLALGFPIASLLYWFTTGLSAGLDAGRLG
                     AGIAATAWLSLLGAVACTLGAVPVGILAARHRGRFTTVIEQVTYAGHALPGIVVALAL
                     VFLGVRVVPWAYQEAPLLVLAYVVLFLPTAVGSVRSSVAQSSVRGEEVARSLGATPGE
                     VLRRVTLPLAAPGISAGAALVLLTCMKELPATLLLRPTGTDTLATSLWTQTGVGAYGA
                     AAPYGLALVVLAVLPTIWLMRASAHDRGSES"
     gene            1095..2162
                     /locus_tag="PL014_11925"
     CDS             1095..2162
                     /locus_tag="PL014_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57845.1"
                     /translation="MNTLSIRDLDVTLGDLAVLRGVHLEVPSGSVTAVLGPSGCGKTT
                     LLRAVAGFVRPDRGEIRVGDTVVSGPGRHLAPERRGIALVPQEGALFPHLSVGDNVAF
                     GLPRAQRRRSPRVAEVLDLVGLRGFQDRSPSELSGGQQQRVALARALAPSPSLVLLDE
                     PFSALDAGLRESVRAQVRDALERSATTALVVTHDQDEALSVADSVAILDEGRILMHAS
                     PQDVYRAPTGLGVARFVGQCVELSAEVSAGVARTALGDLVVEAPGAGGEVDAGRGVVM
                     LRPEQLRLLPVDGVAGTAGEVVGGEYFGHDALVRVRVTAGQGSGQESVEVQVRTLGEI
                     PERGAVRVGVSGPARFYPGAR"
     gene            complement(2144..3157)
                     /locus_tag="PL014_11930"
     CDS             complement(2144..3157)
                     /locus_tag="PL014_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT57846.1"
                     /translation="MRPTSLPEVDYGATSVRPTFSSLPPEVQVACGDAVGSAVVSASP
                     PVGSGFTGAFAGLLHLADGRRVFAKAAGPRMPHVVRGLAAEERILPLIAHLRCPSRLA
                     GAAEVSTADGPWRVLVLDAIDGRQPGAPWSSADADAAATACLEVAAVPSGVAAAVATG
                     TLASDLGGDVEALEALEATAGGTRAWPSDLPPLDGKCLADLAALGLRAERALEGESLV
                     HADTRPDNLLIEPAGTARIVDWNHATLGAPWVDLVALWPLMRHHGVGVHRFAGAPALD
                     GVPEEDIDAFLAILVGYLLCDLDAPPPPGCTPALRRHQLLIAGASTALLAQRRGWVQR
                     APG"
     gene            3258..>3574
                     /locus_tag="PL014_11935"
     CDS             3258..>3574
                     /locus_tag="PL014_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT57847.1"
                     /translation="MSPRARTALQPHATPPEAIRNFCIIAHIDHGKSTLADRMLQITG
                     VVDDRAMRAQYLDRMDIERERGITIKSQAVRMPWDLDGAPHVLNMIDTPGHVDFTYEV
                     SRSL"
BASE COUNT          450 a         1311 c         1298 g          515 t
ORIGIN      
        1 gacccgggcc tggaagaggt cgcccgcagc ctgggccgca ccccgtggca gaccttccgc
       61 gaggtcacgc tgcgccaggt gcgcccgtcc gcggctgccg gtggcctgct cgtcgcgctg
      121 tacacgctca gcgacttcgg ggccgtgtcg atcctgcgct acgacgtctt cacccgcgtg
      181 atctacaccg cgtaccgctc gagtttcgac cgctcgacgg ccgccgtgtt gtccctgctg
      241 ctcgtggcgc tgaccgtcgc gatcagcgtg ggggagtcgc gggttcgccg ccgggacgtc
      301 gcgcgcatcg gggccggggt cgcccgccag ccagagccgc tgcgccttgg ccccctgcgc
      361 ctgccgatgg ccggtggggc cggggtcgtc gtcgtgctgg cgctcggttt cccgatcgcg
      421 tcgttgctct actggttcac caccgggctc tcggccgggt tggatgccgg tcgtctgggt
      481 gccgggatcg cggcgaccgc gtggctgtcg ttgctcgggg ccgtggcctg cacgctgggt
      541 gccgtgcccg tcggcatcct cgcggcccgg caccggggcc ggttcacgac cgtcatcgaa
      601 caggtgacgt acgccgggca cgccctgccc ggaatcgtcg tggcgctcgc cctggtcttc
      661 ctcggggtcc gggtggtgcc gtgggcctac caggaggcgc cactgctggt cctggcctac
      721 gtcgtgctct tcctgccgac cgccgtgggc tccgtgcgca gctcggtggc ccagagctcg
      781 gtgcgcggcg aagaggtcgc ccgctcgctc ggagccaccc ccggcgaggt gctgcgccgc
      841 gtcaccctgc cgctggcagc gccaggcatc agcgcagggg cggccctggt cctgctcacc
      901 tgcatgaagg aacttcccgc gaccctgctc ctgcgcccca ccggcaccga caccctggcc
      961 accagtctgt ggacccagac cggtgtggga gcctacggag cagcagcccc ctacggtctg
     1021 gcactcgtcg tgctcgccgt gctcccgacc atctggctga tgcgcgcatc ggcccacgac
     1081 cgaggaagcg agtcatgaac acgctgtcga tccgtgacct cgacgtcacc ctgggtgacc
     1141 tcgccgtgct gcgcggcgtg cacctcgagg tccccagcgg ctccgtgacc gcggtgctcg
     1201 gcccgtcggg ctgtggcaag acgacgttgt tgcgggcggt cgccggattc gtgcgccccg
     1261 accgtggcga gatccgggtg ggtgacaccg tcgtcagcgg cccgggccgc cacctcgcgc
     1321 ccgagcgtcg cggcatcgcg ctggtccctc aggaaggggc cctgttcccg cacctcagcg
     1381 tcggggacaa cgtcgcgttc gggctcccga gggcgcagcg ccgccgctct ccgcgggtcg
     1441 cggaggtgct cgacctcgtg ggcctgcgtg ggttccagga ccgcagcccc agcgagctgt
     1501 cgggcggaca gcagcagcgc gtcgccctgg cgcgggcgct ggcgcccagc ccgtccctgg
     1561 tgttgctcga cgaaccgttc tccgcgttgg acgccggcct gcgcgagtcg gtgcgcgccc
     1621 aggtccgtga cgccctcgag cgctcggcga ccaccgccct cgtcgtcacc cacgaccagg
     1681 acgaagccct ctcggtggcc gactcggtcg cgatcctcga cgagggccgc atcctcatgc
     1741 acgccagccc gcaggacgtc taccgcgccc ccaccggcct cggggtcgcc cgtttcgtcg
     1801 ggcagtgcgt cgagctgtcg gccgaggtca gcgcaggggt ggcccgcacg gccctcggtg
     1861 acctcgtcgt cgaggctccc ggggcgggtg gtgaggtgga tgccggtcgc ggggtcgtca
     1921 tgttgcgtcc cgaacagctt cgcctcctgc cggtggacgg cgtcgcgggg accgcgggtg
     1981 aggtggtggg cggcgagtac ttcggtcacg acgccctggt ccgggtgcgg gtcaccgcag
     2041 ggcaggggag cgggcaggag agcgtcgagg tgcaggtgcg caccctcggg gagatccccg
     2101 aacgcggtgc agtgcgcgtg ggcgtgagcg gcccggcccg gttctacccc ggcgcgcgct
     2161 gaacccagcc ccgacgctgc gccagaaggg cggtgctggc accggcgatg agcagctggt
     2221 ggcggcgcag cgccggcgtg cagcccggtg gtggtggggc atcgaggtcg cacagcaggt
     2281 atcccacgag gatcgccagg aacgcgtcga tgtcctcctc cggcacgcca tcgagggccg
     2341 gagcccctgc gaagcggtgc acgcccacgc cgtggtggcg catgagaggc cacagggcga
     2401 cgaggtcgac ccagggcgcg ccgagggtcg catggttcca gtcgacgatg cgggctgttc
     2461 cggctggttc gatgaggagg ttgtcggggc gcgtgtcggc atgcaccagg gactcgccct
     2521 ccagtgctcg ctcggcgcgc aggcccaggg ccgcgaggtc ggcaaggcac ttcccgtcga
     2581 gagggggaag gtcgctcggc caggcccggg ttccgcccgc cgtcgcctcg agggcctcca
     2641 gcgcctcgac atcgcccccg aggtcggacg ccaacgtgcc ggtcgccacc gccgctgcga
     2701 cccctgacgg gaccgcggcc acctccaggc aggcggtggc cgcggcatca gcgtcggctg
     2761 agctccacgg tgcgccgggt tgccgcccgt cgatcgcatc gagcacgagc acgcgccacg
     2821 gcccatccgc ggtcgacacc tctgctgctc cggcgagccg gctcgggcag cgcaggtgcg
     2881 cgatcagcgg aaggatgcgc tcttctgcag cgagccccct gacgacatgg ggcatccgcg
     2941 gcccagcggc cttggcgaac acccgtcggc cgtccgcgag gtggagcagc ccggcaaagg
     3001 cgccggtgaa ccccgaaccc acgggaggtg aggcgctcac gacggcgctg ccgacggcat
     3061 ccccgcatgc gacctgaacc tcgggcggca gcgaggagaa ggtcggccgc accgacgtgg
     3121 cgccgtagtc gacctcggga agggatgtcg ggcgcacccg tccaccttcg ccgcgggggc
     3181 gcgccggtgg caaccgagat tcgtgggaga ctggggaaca gtgccctgac ccctccaacg
     3241 caaggaccca tccgctcgtg tcaccccgtg cccgcaccgc gctgcagccg cacgccacgc
     3301 cgccggaggc gatccgcaac ttctgcatca tcgcccacat cgaccacggc aagtcgaccc
     3361 tggccgaccg gatgctgcag atcaccggcg tcgtcgacga ccgggcgatg cgcgcccagt
     3421 acctggaccg catggacatc gagcgcgagc gcggcatcac catcaagagc caggcggtgc
     3481 ggatgccgtg ggacctcgac ggggccccgc acgtgctcaa catgatcgac acgcccggtc
     3541 acgtcgactt cacctacgag gtcagccgca gcct
//
LOCUS       DAOSOC010000213         3562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_26314_length_3562_cov_9.952666, whole genome shotgun sequence.
ACCESSION   DAOSOC010000213 DAOSOC010000000
VERSION     DAOSOC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26314_length_3562_cov_9.952666"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1323)
                     /locus_tag="PL014_11940"
     CDS             complement(4..1323)
                     /locus_tag="PL014_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT57848.1"
                     /translation="MARDDRAHVFHSWSAQALIAPLPIAGASGSHFWDYDGKKYLDFS
                     SQLVNVNIGYQHPKLVAAIQEAAARQCTIAPSFADESRSEAARLIAERAPGDLDKVFF
                     TNGGAEATENAMRMARLHTGRHKVLTTYRSYHGATSGSITATGDPRRWANEPGMPGIV
                     HFWGPYPYRSQFHAADEAQECERALQHLRDVIMVEGPSTIAAIMLETIVGTNGILIPP
                     DGYLAGVREICDEHGIVWIADEVMSGFGRCGEWFAVDHWGVTPDLICFAKGVNSGYVP
                     LGGVVISAAIAATFEQRAFPGGLTYSGHPLACASAVASIRIFEEEGILENVRKVSDTV
                     LAPRLAEIAAKHPSVGEVRGLGFFWGLELVRNPETREPLVPFNASGAAAGPMNEFAAA
                     CKAEGLWPFVHFNRTHVVPPCTTTAEEMNEGLDILDRALEVADRFTA"
     assembly_gap    1349..1448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1449..2920)
                     /locus_tag="PL014_11945"
     CDS             complement(<1449..2920)
                     /locus_tag="PL014_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HOT57849.1"
                     /translation="MHQYIGGRRRDASSGETFDVLDPSTNETLETVTLAGHNDVDAAV
                     AAASEAFEQWSRATPAERSTVLLKAAQLLDERAEELAQLESRQAGKPIRLAREFDVPG
                     TVDNTAFFGAAARRLDGMASAEYSGDHTSSIRREPIGVVGSIAPWNYPLQMAAWKVLP
                     AIAAGNTIVLKPAEITPLTSVLFAEAFTEAGAPPGVVNVVTGTGPVAGAHLVGHPDVD
                     MTSFTGSTAVGRQVMATAAATGKRVHLELGGKAPFVVFDDADLEAAIHGAVAASLINT
                     GQDCTAATRAIVHRSLHDEFVRGVADLMAAVRMGPTADEATDLGPLSSAAHQAKVAGM
                     VERARGYATVVTGGRTPEGDLARGCYYEPTLITDVPLDSEIWRDEVFGPVLAVVPFDD
                     DDEAIRLANDTPFGLAASAWSRDTYRTARATREIKAGCVWINDHIPIISEMPHGGYRH
                     SGFGKDMSAYSFDEYTNVKHVMHDVTAVAHKPWHRTIFTLP"
     gene            complement(3030..3551)
                     /locus_tag="PL014_11950"
     CDS             complement(3030..3551)
                     /locus_tag="PL014_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOT57850.1"
                     /translation="MSLDRPVAPNPYDLLPRVASFTVTSTDVTDGAPLKDDQVAHLGN
                     TSPQLSWSGAPEGTQSYVITCFDPDAPTPSGFWHWVLVDVPAGVTELATGAASGELPA
                     GAFHVRHDGGGHGFMGAAPPEGDQVHRYFFVVHAVNAPTLGVDADASPAVVSFNLAFK
                     TLGRAIIHGTYQH"
BASE COUNT          487 a         1128 c         1329 g          518 t
ORIGIN      
        1 ccatcaggcc gtgaaccggt cggccacctc aagggcccgg tcgaggatgt cgagcccctc
       61 gttcatctcc tccgcggtgg tggtgcacgg cggcacgacg tgggtgcggt tgaagtgcac
      121 gaacggccac agaccctcgg ccttgcacgc ggcggcgaac tcgttcatcg ggcctgcggc
      181 agcaccggag gcgttgaacg gcacgagcgg ctcgcgggtc tcggggttgc ggacgagctc
      241 gagcccccag aagaagccca acccgcgcac ctcgccgacg ctggggtgct tggcggcgat
      301 ctcggccagg cggggagcca gcacggtgtc gctgaccttg cggacgttct cgaggatgcc
      361 ctcctcctcg aagatccgga tcgaggccac cgccgaggca cacgccagcg ggtggcccga
      421 gtaggtcagc ccgcccggga aggcccgctg ctcgaacgtc gccgcgatcg cggccgagat
      481 gacgaccccg ccgagcggca cgtagcccga gttcacaccc ttggcgaagc agatgaggtc
      541 gggggtgacc ccccagtggt cgacggcgaa ccactcgccg cagcgcccga acccggacat
      601 gacctcgtcg gcgatccaca cgatcccgtg ctcgtcgcag atctcgcgga ccccggcgag
      661 gtagccgtcc ggcggaatga ggatgccgtt ggtcccgacg atggtctcga gcatgatcgc
      721 cgcgatcgtc gacggaccct cgaccatgat gacgtcgcgc aggtgctgca gggcgcgctc
      781 gcactcctgc gcctcgtcgg cggcgtggaa ctgcgagcgg taggggtagg ggccccagaa
      841 gtggacgatc cccggcatcc ccggctcgtt cgcccagcgg cgggggtcac ccgtcgcggt
      901 gatcgatccc gaggtggccc cgtggtagct gcggtaggtc gtgagcacct tgtggcgacc
      961 ggtgtgcagg cgcgccatgc gcatggcgtt ctcggtggcc tcggccccgc cgttggtgaa
     1021 gaacaccttg tcgaggtcgc ccggggcgcg ctcggcgatc agcctggccg cctcggagcg
     1081 ggactcgtcg gcgaagctcg gggcgatggt gcactgacgg gccgcggcct cctggatcgc
     1141 cgcgacgagc ttggggtgct ggtagccgat gttgacgttg acgagctggc tggagaagtc
     1201 gaggtacttc ttgccgtcgt agtcccagaa gtggctgccc gacgcccccg cgatgggcag
     1261 gggtgcgatg agcgcctgcg ccgaccacga gtggaagacg tgggcgcggt cgtcacgcgc
     1321 cacggcggcg ccccgctcgg cgtcggcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnngg cagggtgaag atcgtgcggt gccagggctt gtgggccacg gcggtgacgt
     1501 cgtgcatgac gtgcttgacg ttggtgtact cgtcgaagga gtaggccgac atgtccttgc
     1561 cgaacccact gtgccggtac ccgccgtgcg gcatctcgct gatgatcggg atgtggtcgt
     1621 tgatccacac gcagccggcc ttgatctcgc gggtggcccg ggcggtgcgg tacgtgtcgc
     1681 gcgaccacgc ggacgcggcc agcccgaacg gggtgtcgtt ggccaggcgg atcgcctcgt
     1741 cgtcgtcgtc gaacggcacg acggccagca ccggcccgaa cacctcgtcg cgccagatct
     1801 cgctgtcgag cggcacgtcg gtgatgaggg tcggctcgta gtagcagcct cgggcgaggt
     1861 cgccctcggg ggtgcggccg ccggtgacga cggtggcgta gccgcgggca cgctcgacca
     1921 tgccggcgac cttggcctgg tgggcggccg agctgagcgg gccgaggtcg gtcgcctcgt
     1981 cggccgtcgg gcccatccgc accgcggcca tgaggtcggc cacaccccgc acgaactcgt
     2041 cgtgcagcga gcggtggacg atggcgcgcg tcgcggcggt gcagtcctga ccggtgttga
     2101 tcagggatgc cgcgacggca ccgtggatgg ccgcctcgag gtcggcgtcg tcgaacacga
     2161 cgaacggcgc cttgccgccg agctcgaggt gcacgcgctt gccggtggca gccgcggtgg
     2221 ccatcacctg acgaccgacg gccgtcgacc cggtgaacga ggtcatgtcg acgtcggggt
     2281 ggccgacgag gtgggcgccc gcgaccggcc ccgtgccggt gacgacgttg acgactccgg
     2341 ggggtgcgcc ggcctcggtg aacgcctcgg cgaacagcac cgacgtcagc ggggtgatct
     2401 cggcgggctt gaggacgatg gtgttgccgg cggcgatcgc gggcaggacc ttccaggcgg
     2461 ccatctgcag cgggtagttc cacggggcga tcgacccgac gacgccgatg ggctcgcgcc
     2521 ggatcgagct cgtgtggtca ccggagtact cggcgctggc catgccgtcg aggcgacggg
     2581 cggcggcacc gaagaacgcg gtgttgtcga cagtgccggg tacgtcgaac tcgcgggcga
     2641 ggcggatcgg cttgcccgcc tggcggctct cgagctgcgc gagctcctcg gcgcgctcgt
     2701 cgagcagctg ggcggccttc aggagcacgg tcgagcgctc ggcgggggtg gcccggctcc
     2761 actgctcgaa ggcctcggat gccgcggcca ccgccgcgtc gacgtcgtta tggcccgcga
     2821 gggtcaccgt ctccagggtc tcgttcgtgc tcgggtcgag gacgtcgaag gtctcgccac
     2881 tggatgcgtc acgccgtcgg ccgccgatgt actggtgcat gacgcgagca tacgcaccgg
     2941 cgcggacata ggaaaagcga acccagaggc gcgtggggct acggaatccg tcgcgaggtg
     3001 agcaacggca tccgatctcg ttcgtgcgat cagtgctggt aggtgccgtg gatgatcgcc
     3061 cgaccgaggg tcttgaaggc gaggttgaac gacacgacgg ccgggctcgc gtcggcgtca
     3121 accccgagcg tcggggcgtt caccgcgtgg acgacgaaga agtagcggtg cacctggtcg
     3181 ccctccggcg gggcggcgcc catgaagccg tggccgccgc catcgtgccg cacgtggaag
     3241 gcgccggccg ggagctcgcc cgaggccgca ccggtggcca gttcggtgac gccggcgggg
     3301 acgtccacga gcacccagtg ccagaagccg gacggggttg gggcgtcggg gtcgaagcac
     3361 gtgatgacat agctctgcgt gccctcgggg gcgccgctcc acgacagctg cggggaggtg
     3421 ttgccgaggt gggcgacctg gtcgtccttc agcggggcgc cgtcggtgac gtcggtgctc
     3481 gtcaccgtga agctggcgac ccggggaagc aggtcgtagg ggttcggggc gacggggcgg
     3541 tcaaggctca tcgggtgctc ct
//
LOCUS       DAOSOC010000214         3555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_26372_length_3555_cov_9.374571, whole genome shotgun sequence.
ACCESSION   DAOSOC010000214 DAOSOC010000000
VERSION     DAOSOC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26372_length_3555_cov_9.374571"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..74)
                     /locus_tag="PL014_11955"
     tRNA            complement(2..74)
                     /locus_tag="PL014_11955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Lys,seq:ttt)
     gene            complement(204..1298)
                     /locus_tag="PL014_11960"
     CDS             complement(204..1298)
                     /locus_tag="PL014_11960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOT57851.1"
                     /translation="MNRFHEGFVLGDRYTLTGRIASGGMGDVWEASDSVLERQVAVKI
                     LRPSLEEEEVFALRFRNEALHTANLAHVNVATVFDYGEEDNLAYLVMELVPGEALSDL
                     VRREGALPPDQVRAILAQAALALGAAHEAGVVHRDVKPANILVMPDGTVKLTDFGIAR
                     AVDGSGHTQTGEVLGTPHYLSPEQALGEAATGASDLYALGIVGHELLTGQRPFDKGTP
                     VATALAQVNDPPPPLPTHVPPGLRSLIEQCLEKKPSDRPASARELGERLGLPEADLPV
                     ATEDAAQVVGTAAAQEQSADEHSASEHSAGEPSPDDPTFTPRHWSGGDPTPTPSSLPP
                     VPKGHVHWAWVPGFSVALLAVLAAFYLVTR"
     gene            complement(1398..2591)
                     /locus_tag="PL014_11965"
     CDS             complement(1398..2591)
                     /locus_tag="PL014_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOT57852.1"
                     /translation="MTQHDTAAAKAHTAPPPDADRKPDSPGDIDRPGWRYTAKASVAE
                     FQRDQCTDLAAALTYYSVLSVFPAILALVSLLGVFGQGEATTQALLEILRDLGQSDIA
                     DQLEKPIAAMVEAQSAGLALAVGLAGALWGASGYVGAFGRTLNRIYQVDEGRPVWKLR
                     PLVLLVTVGLVVMAAIVLVGLVVSGPVAEAIGAAIGFGEQSLALWNLAKWPFLLAVVV
                     LMVAVLYYATPNVQQPKFRWVSVGAAVAIGIWVIASLGFGFYVTNFAKYDSLYGSLGS
                     VIVFLLWLWLTNLALLFGAEIDAELERARQLQAGIRAERTLQLPPRDDSGSLKAATKL
                     EERVAEGRRLRLEAQASGEVEDSAIGYGRGHGHQRRRARSGEAPAHELEPLPSEIARA
                     RDRRP"
     gene            2787..3371
                     /locus_tag="PL014_11970"
     CDS             2787..3371
                     /locus_tag="PL014_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57853.1"
                     /translation="MESVGGTLPGPAEVTEIAHQSAAILVGAGRATHDPEVTGRLVTL
                     VDDLGLSTVADLWSGRPARSLPGTLWRLYALREWVQRSPEQASREYAAGIRFTDVAHA
                     VAGIAEPPRPEELQRVADQILTGVFEGDLAVALERASAFCSVVAAGRAELAHDRDHVD
                     PDGASDLTRTALAMLTTGEDLRACARLWRAGDLH"
BASE COUNT          505 a         1282 c         1282 g          486 t
ORIGIN      
        1 gtgggccccg tgggactcga acccacaacc tacggattaa aagtccgcag ctctgaccat
       61 tgagctagag gcccggagga cgagacggca tccacgcacc gtgcgccacg gcatccgagg
      121 ttcggggccg cacgcgtccg cggacagtat ggcgtagccc ggctcggatc acgccgcccg
      181 gtccggcgcc ggatgccacc ggttcagcgc gtgacgaggt agaacgcggc caggacggcg
      241 agcagggcga cgctgaaccc gggcacccag gcccagtgga cgtggccctt cggcacgggc
      301 ggcaggctcg acggggtggg ggtggggtcg ccgccggacc agtgccgcgg ggtaaaggtc
      361 gggtcgtcgg gtgacggctc cccggccgag tgctcgctgg ccgagtgctc gtcggcgctc
      421 tgctcctgcg ccgcggcggt accgacgacc tgcgcagcgt cctcggtggc aacgggcagg
      481 tccgcctcgg gcagcccgag acgctcaccg agctcacggg cgctcgccgg ccggtcggac
      541 ggcttcttct cgaggcactg ttcgatgagc gaccgcaggc ccgggggaac gtgcgtcggc
      601 agcggcgggg gcgggtcgtt gacctgggcg agtgccgtgg cgaccggcgt ccccttgtcg
      661 aagggccgct gtccggtgag cagctcgtgg cccacgatcc cgagggcgta caggtcggac
      721 gctccggttg ccgcctcgcc gagggcctgc tcgggggaga ggtagtgcgg ggtgccgagc
      781 acctcgccgg tctgggtgtg ccccgacccg tcgacggccc gggcgattcc gaagtccgtg
      841 agcttcacgg tgccgtcggg catgacgagg atgttggcgg gcttgacgtc gcggtggacg
      901 acaccggcct cgtgggccgc cccgagggcc aacgccgcct gcgccaggat cgcgcgcacc
      961 tggtcgggcg gcagagcgcc ctcgcgtcgc acgaggtccg acagggcctc tccgggcacg
     1021 agctccatga cgaggtaggc gaggttgtcc tcctcgccgt agtcgaagac ggtggcgacg
     1081 ttgacgtgcg cgaggttggc ggtgtgcagc gcctcgttgc gaaagcgcag cgcgaagacc
     1141 tcttcctcct ccaggctcgg ccgcaggatc ttcacggcca cctgacgctc gagcacgctg
     1201 tcgctggctt cccagacgtc gcccatcccc ccggacgcga tccggcccgt cagcgtgtag
     1261 cggtcgccga ggacgaatcc ctcgtgaaac cggttcaccg ggggttggtc gcattcacct
     1321 gaccatggta ggtcaccaca gggcccctga ccggcctgcg tacgcgcggg tcacgtgccg
     1381 ggaggccgag gactccctca ggggcgccgg tcccgggccc gcgcgatctc cgacggcaag
     1441 ggctccagct cgtgtgccgg tgcctcaccg gagcgggccc tgcgtcgttg gtgtccatga
     1501 ccccgtccgt acccgatcgc ggagtcctca acctcgcccg acgcctgcgc ctcgaggcgc
     1561 agccgacgcc cctcggcgac gcgctcctcc agcttcgtgg cggccttgag cgagccggag
     1621 tcatcgcgcg gcggcagctg cagcgtgcgc tcggcccgga tacccgcctg cagctgccgt
     1681 gcccgctcca gctcggcgtc gatctcggcc ccgaagagca gtgcgaggtt ggtcagccac
     1741 agccacagga ggaagacgat gacgcttccg agggagccgt agagcgagtc gtacttcgcg
     1801 aagttcgtga cgtagaaccc gaaccccagc gaggcgatca cccagatgcc gatcgcgacg
     1861 gcagccccca ccgagaccca ccggaacttg ggctgctgca cgttcggcgt cgcgtagtac
     1921 aagaccgcga ccatcagcac gacgactgcg aggaggaacg gccacttggc gaggttccac
     1981 aacgccaggc tctgctcgcc gaacccgatc gccgcgccga tcgcctcggc gacgggcccg
     2041 ctgacgacaa ggccgaccag gacgatggcc gccatgacga ccaggcccac cgtcacgagg
     2101 agcacgagcg ggcgcagctt ccagaccggg cggccctcgt cgacctggta gatccggttc
     2161 agcgtgcgac cgaacgcccc gacgtagccc gacgcccccc agagggcacc ggcgagaccg
     2221 acggccagcg ccaggccggc gctctgcgcc tcgaccatgg ccgcgatggg cttttccagc
     2281 tggtcggcga tgtcgctctg gccgaggtcg cgcaggatct cgagcagcgc ctgggtggtt
     2341 gcctccccct gcccgaagac cccgaggagg gagaccagcg ccaggatcgc ggggaagacc
     2401 gagagcaccg agtagtacgt cagggccgcc gccaggtcgg tgcactggtc gcgctggaac
     2461 tcggcgaccg aggccttcgc cgtgtacctc caccccggac gatcgatgtc gcccggggag
     2521 tcgggcttgc ggtccgcgtc gggtgggggc gccgtgtggg ccttcgccgc ggcggtgtcg
     2581 tgctgggtca tgtccgtgct cctaccccgg gtggtcggcc tcgcacacga ggtggtgacc
     2641 tagtcaacct aacccgcgac acggcatccg gatgccgtca tgggcggccc tgctcggatc
     2701 gggaacggtg ctgggatgat gaggcggtga gcaccgacgg cggacgaccc ctcccgcgcc
     2761 ggccccagcc actgggcccc gacgcgatgg agtcggtcgg cggcaccctg cccgggccgg
     2821 ccgaggtcac cgagatcgcg caccagagcg cggcgatcct cgtcggtgcc ggccgcgcca
     2881 cccacgaccc cgaggtgacc gggcgcctgg tcaccctcgt cgacgacctc gggctgtcca
     2941 ccgtcgccga cctgtggagc gggcgacccg cgcgctcgct gcccggcacg ttgtggcgcc
     3001 tgtacgccct gcgcgagtgg gtgcagcgca gccccgagca ggccagccgc gagtatgccg
     3061 ccggcatccg gttcaccgac gtcgcccacg ccgtcgccgg catcgccgag cccccgcgcc
     3121 ccgaggagct ccagcgggtc gccgaccaga tcctcaccgg ggtgttcgag ggcgacctgg
     3181 ccgtcgccct ggaacgggcc tcggcgttct gctccgtcgt cgcggcgggc cgtgccgagc
     3241 tggcgcacga ccgtgaccac gtcgaccccg acggtgccag cgacctcacc cgcacggccc
     3301 tggcgatgct caccaccggg gaggacctgc gggcctgtgc ccggctctgg cgggccggcg
     3361 acctccactg aggtcgtggg gtcgcgggtg ctcgtgagcg gtcgaccttc aacgaagtga
     3421 cgacgtcggg aacacctgga cggcgatgag gcgttaatgt ctcccgtggg tgccggaccg
     3481 cggcagcccc gggtcccaac atcagccgct acgagcggcc acgcgccgtg aggcgctccc
     3541 ggtccggcac ccact
//
LOCUS       DAOSOC010000215         3531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_26610_length_3531_cov_10.287112, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000215 DAOSOC010000000
VERSION     DAOSOC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26610_length_3531_cov_10.287112"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /gene="fdhF"
                     /locus_tag="PL014_11975"
     CDS             <1..1773
                     /gene="fdhF"
                     /locus_tag="PL014_11975"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOT57854.1"
                     /translation="AFGNNNIDTCARVCHSPTGYGLNHAFGTSAGTQDFASVEQADVM
                     LVIGANPTDAHPVFASRMKKRLRAGAKAIVADPRRIDLVRSPHVEAALHLPVRPGTNV
                     AFVNAMCHVIVTEGLWDEPFVRERCEDADAYLAFIAGPEHSPEATEDVTGIPASDLRA
                     AARLFAAGPNSAIYYGLGVTEHSQGSTMVMGMANLAMLTGNLGREGVGVNPLRGQNNV
                     QGSCDMGSFPHELPGYRHVSRPEVREIYESLWGVTLQAEPGLRIPNMFDSAIGGSFKG
                     LYVQGEDIAQSDPNTTHVEAALRAMDLVVVHDLFLNETARFAHVFLPGASFLEKDGTF
                     TNAERRINRVRPVMPSRVGRDEWQVVCEIATAMGYPMSYAAPSHIMDEIAATTPTFAG
                     VSFARLDAEGSMQWPVTEAEPHGTPTMHVGSFVRGLGQLTPTVYVPTTERANRRFPLV
                     LTTGRILSQYNVGAQTRRTPNSTWHPEDVLEIHPSDAELRGVTSGDVVELSSRVGTTR
                     LTAQVSDRMPAGVVYTTFHHPVTGANVVTTENSDWATNCPEYKVTAVQVQVVTQAARA
                     EAGAEAELRSDAGAPVPQPVAGRS"
     gene            1800..2027
                     /locus_tag="PL014_11980"
     CDS             1800..2027
                     /locus_tag="PL014_11980"
                     /inference="COORDINATES: protein motif:HMM:NF022832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HOT57855.1"
                     /translation="MGHDLVRNLEHLPAETATMEIATHIRKFWEPRMRHELLARVRWG
                     DTTLHPLLVAAAGDLVDGEVDRSEIREPSGG"
     gene            complement(2072..2467)
                     /locus_tag="PL014_11985"
     CDS             complement(2072..2467)
                     /locus_tag="PL014_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57856.1"
                     /translation="MAKQLRARGGEPNGHVSRSLTTEMLALADLVVTFEFAQRMRILE
                     SHPGWSHKLFGLHQLADALERTTTARTGFWLVHEALRASSPDSMTWDVADPYGRGATA
                     ARRAADEIDAALSVIVPALTRVPAGGQQR"
     gene            2785..>3531
                     /locus_tag="PL014_11990"
     CDS             2785..>3531
                     /locus_tag="PL014_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOT57857.1"
                     /translation="MRLSHTLAATSAAFDDPNLVSAAGLVPAMALAQRAGLADLADEH
                     LSVPTDKGANAGLKVASLVAGMVAGADSIDDMALLRHGGMGRVFARAYAPSTLGSFLR
                     AFTFGHVRQLDAVASRFLFALTRLVGFAPPAPDCTQGADDTAGVGGGFAFVDVDDTII
                     EVHGHAKQGAGFGYTRVRGLNALIATLTTPAAAPLVVAQRLRKGACGSPRGAQRLVGD
                     AVKQAHRLLGDDRPVLVRMDSAYYGRDAVHA"
BASE COUNT          510 a         1251 c         1255 g          515 t
ORIGIN      
        1 gcgttcggca acaacaacat cgacacctgt gcgcgggtgt gccactcccc caccggctac
       61 gggctcaacc acgccttcgg cacctccgcc ggcacccagg acttcgcctc ggtcgagcag
      121 gccgacgtca tgctcgtcat cggcgccaac cccaccgacg cccacccggt gttcgcgtcg
      181 cggatgaaga agcggctgcg tgcgggcgcc aaggccatcg tcgccgaccc gcgacgcatc
      241 gacctcgtgc gttccccgca cgtcgaggcc gcgctgcacc tgcccgtgcg ccccggcacc
      301 aacgtcgcct tcgtcaacgc gatgtgccac gtcatcgtca ccgaggggct gtgggacgag
      361 cccttcgtgc gcgagcgctg cgaggacgcc gacgcctacc tggccttcat cgcggggccc
      421 gagcactcgc cggaggcgac ggaggacgtg accggcatcc cggcatccga cctgcgtgcc
      481 gcggcgcggc tgttcgccgc cgggccgaac tcggcgatct actacgggct cggcgtcacc
      541 gagcacagcc agggctcgac gatggtcatg ggcatggcca acctcgcgat gctcaccggc
      601 aacctcgggc gcgagggtgt gggcgtgaac ccgctgcgcg ggcagaacaa cgtccagggc
      661 tcgtgcgaca tggggtcctt cccccacgag ctgcccggct accggcacgt gtcgcggccc
      721 gaggtgcgcg agatctacga gtcgctgtgg ggggtcaccc tccaggccga gccggggctg
      781 cggatcccga acatgttcga ctccgcgatc ggcggctcgt tcaaggggct gtacgtgcag
      841 ggcgaggaca tcgcccagag cgaccccaac accacccacg tcgaggccgc gctgcgcgcg
      901 atggatctcg tcgtcgtgca cgacctgttc ctcaacgaga cggcgcggtt cgcgcacgtc
      961 ttcctccccg gcgcctcgtt cctcgagaag gacggcacgt tcaccaacgc cgaacgccgg
     1021 atcaaccggg tgcggccggt catgccgagc cgggtcggca gggacgagtg gcaggtcgtg
     1081 tgcgagatcg cgacggcgat gggctacccc atgtcgtatg ccgcgccgtc gcacatcatg
     1141 gacgagatcg ccgccacgac accgacgttc gcaggtgtct cgttcgcgcg cctcgatgcc
     1201 gaggggtcga tgcagtggcc ggtcacggag gccgaaccgc acggcacgcc gaccatgcac
     1261 gtcgggtcgt tcgtgcgagg cctcgggcag ctgacgccga cggtgtacgt gcccaccacc
     1321 gagcgggcca accggcgctt ccccctggtg ctcacgaccg ggcgcatcct ctcgcagtac
     1381 aacgtcggcg cccagacccg gcgcaccccg aactccacgt ggcaccctga ggacgtcctc
     1441 gagatccatc cctcggatgc cgagctgcgg ggggtgacgt cgggcgacgt cgtcgagctg
     1501 tcgtcgcggg tcgggaccac gcgcctgacg gcgcaggtct ccgaccggat gccggccggc
     1561 gtggtctaca cgacgttcca ccaccccgtc accggggcca acgtcgtgac gactgagaac
     1621 tccgactggg cgaccaactg ccccgagtac aaggtgacgg ccgttcaggt ccaggtcgtg
     1681 acccaggcgg cgcgcgcgga ggccggtgcc gaggcagagc tgcgctcgga tgccggggcg
     1741 ccggtgccgc agcccgtggc gggtcgctcg tgagtggcga cgtgccggcg gtggtgagga
     1801 tggggcacga cctcgtccgc aacctggagc accttccggc tgagacggcc acgatggaga
     1861 tcgcgacgca catccgcaag ttctgggagc cgcgcatgcg tcacgagctg ctcgcccgcg
     1921 tccggtgggg cgacacgacg ctgcacccgc tgctcgtggc cgcggccggc gacctcgtcg
     1981 acggcgaggt cgaccgctcc gagatcaggg agccctccgg aggctgagat cggtctgcgc
     2041 accacgccct gctcaggcca gaccgaccgg ctcaccgctg ctggccgccg gcgggcactc
     2101 gagtcagggc ggggacgatg accgacaggg ccgcgtcgat ctcgtctgcg gccctgcgtg
     2161 cggcggtcgc tcccctgccg tacgggtcgg cgacgtccca cgtcatcgag tccggggagc
     2221 tcgctcggag cgcctcgtgc acgagccaga acccggtcct ggccgtcgtg gtccgttcga
     2281 gcgcgtcggc gagctggtgg agcccgaaga gcttgtgaga ccagcccggg tgcgactcga
     2341 ggatccgcat gcgctgcgcg aactcgaacg tcacgacgag gtccgccagc gcgagcatct
     2401 cggtggtcaa ggaacggctg acgtgtccgt tcggctcgcc gccccgagcc ctcagctgct
     2461 tggccatctc tgggtccatt ccccgcccgg gatgaccggg gatgccggcg ctggccgccg
     2521 tgactgggtg caggggccca cccacccccc ccgaactggt gcgctgcgcg gcgctcggcg
     2581 tacggcgagc gggagatgtt ggcggtacac acgaacaaca gacgcagcgg agccggcgag
     2641 gacgcgggcg tcggggtcac ttagcctcga tccaccgatg aagtcggtcg ggtgagcgtg
     2701 ccgtaacaac gaagtgccct gccgtgctgg gagattggga cttgcgacgg ttccaaaacc
     2761 tcaacccgga agggcacctc tgggatgcga ctctctcaca ctctggctgc gacatcggcg
     2821 gcgttcgacg acccgaatct ggtgtcggcg gcggggctgg tcccggccat ggcgttggcg
     2881 cagcgggccg ggttggccga ccttgctgat gagcatctga gcgtgccgac ggacaagggc
     2941 gcgaacgcgg ggttgaaggt ggcctcgctg gtggcgggga tggtcgccgg cgccgactcc
     3001 atcgatgaca tggcgctgct gcggcacggg gggatgggcc gggtcttcgc ccgggcgtac
     3061 gccccgtcga cgttggggtc gttcctgcgg gcgttcacct tcgggcacgt gcgccagctc
     3121 gacgctgtcg cctcccggtt cctgttcgcc ctgacccgcc tggtcgggtt cgccccacct
     3181 gccccggact gcacgcaggg tgccgacgac accgcgggcg tgggcggtgg gttcgcgttc
     3241 gtcgacgtgg acgacacgat catcgaggtc cacggccacg ccaagcaggg tgccgggttc
     3301 gggtacaccc gggtccgcgg cctgaacgcg ctgatcgcca cgctgaccac gcccgcagcg
     3361 gcgccgctgg tcgtggcgca acggctgcgc aagggtgcct gcgggtcacc gcgcggcgcg
     3421 caacgcctgg tcggtgacgc ggtgaaacag gcccaccgcc tcttgggcga tgatcgcccg
     3481 gtgctggtgc ggatggactc ggcctactac ggccgcgacg ccgtccacgc c
//
LOCUS       DAOSOC010000216         3531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_26611_length_3531_cov_7.063867, whole genome shotgun sequence.
ACCESSION   DAOSOC010000216 DAOSOC010000000
VERSION     DAOSOC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26611_length_3531_cov_7.063867"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1683
                     /locus_tag="PL014_11995"
     CDS             271..1683
                     /locus_tag="PL014_11995"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOT57858.1"
                     /translation="MELIDYWRILRRHWLGVVAIVLATVAIAAAYTLAQPKVYSANAN
                     GFVSTGPADNPALSSVNDQLAKSRATSYVDIATSRATAQAVIEDLGLDASPAGLVGSI
                     SVTQPKDTVLLKITADASTPEAAQQLADAWVRALATQVQKIEDPSGTGAPGVPQVLPV
                     ESAAVPTAPASPNPRLNLMLGAVLGLGLALGYAMLRHTVDRRLRSKEEIEAQFQISVV
                     GGVPVSQVLKKDARGGHGHLAVDAAVSAANGPASEAFRKLRTNLMYMNVDNPPRVVVV
                     TSPLPGDGKSTVAANLAAAIDSSGENVVLIDGDLRRPTVATWFGLVEGVGLTDVLAGR
                     IPVEDALQTPVEHPNLRVLGAGAIPPNPSELLGSQVMRQLLQKLSADALVLIDAPPLL
                     PVTDAALLTANADGALVVVSAGKTLDTQLGSALDHLSDVEAKALGVVLNRVSPRDAGN
                     GYYDQYSGYYTQPGRRRSKK"
     gene            1680..1964
                     /locus_tag="PL014_12000"
     CDS             1680..1964
                     /locus_tag="PL014_12000"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOT57859.1"
                     /translation="MTHTGEAVWRHRGEVAAVDSGDRVAVLDLTSLAVPPRILEGPAA
                     AIWRAVDGERATPEVVQAVAQSFGVEAAEIEADVLAFLASLSSAGLLEAS"
     gene            1964..2884
                     /locus_tag="PL014_12005"
     CDS             1964..2884
                     /locus_tag="PL014_12005"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT57860.1"
                     /translation="MVDTALRLRADEAVPLLHTLVARAAADLGLRVLAIKGPVAALQG
                     LRKPRPSADLDVLVHPRDLESLVAGLVALGWRRGVEGTYASIMPPHSVNLLSDLWPVA
                     MDVHHYFPGFLADAGAVFDVLWAGHELVTLAGVPVPACDPVGQVAVLALHHLRISPDG
                     DSPALRELSSRAGATLSADEVRALVQLAHATDATATLRPFLLALGVPDAEIGPARDAI
                     ALDAWNRRVRAVGYEAWFLKFARTPKRRWPRTLWHALVLTDDEIHVYSNVPPGSGGLL
                     RLRLRRIGRGLAGVPRTVRAMARERRRGSG"
     gene            complement(2889..3425)
                     /locus_tag="PL014_12010"
     CDS             complement(2889..3425)
                     /locus_tag="PL014_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HOT57861.1"
                     /translation="MPRVPITVLFVCTANICRSAYAEAAARHLAGSRTDVVFSSAGTH
                     GYVSQPMDAEMARFLPEGGAHDGFASRRVTRDLIDDADLVLTAEASHRTFLLEEYPHA
                     FRKVLTLGQFAEAAGHTELRGRELITAVGLRQPPARPEHDVRDPYRRGSAANARSAAQ
                     IDELLTIVVPALVDETTR"
BASE COUNT          472 a         1273 c         1290 g          496 t
ORIGIN      
        1 gacggtggtg gtcgccggag cgcctcggcc ggcgaggcac cggtcccagg cggtcgcgat
       61 ggcgtcggcg agaggggtgg cccctccccc gcccaccacg acgtcgaccc gcgtccccaa
      121 caggtccagg gccacgcgcg gccggctcgc ggtgcccggg agtgtcacgg acgcattgta
      181 gactcgtccc gaatcgccgg atgtccgcgc acgagagcgc ccatcggctc gacaaggggc
      241 tactcggctc gacaaggggc tactccattc atggaactca tcgactactg gcggatcctg
      301 cgccggcact ggctgggagt cgtggcgatc gtgctcgcca cggtggcgat cgccgcggcc
      361 tacaccctgg cccagccgaa ggtgtactcg gccaacgcga acgggttcgt ctccacgggc
      421 ccggccgaca accccgcgct gagctcggtc aacgaccagc tcgccaagtc ccgggccacc
      481 tcctacgtcg acatcgccac gagccgggcc actgcccagg cggtcatcga ggacctcggc
      541 ctcgacgcca gcccggccgg cctggtgggc agcatctcgg tgacgcagcc caaggacacc
      601 gtgctgctca agatcaccgc cgacgcgagc acccccgaag cggcccagca gctggctgac
      661 gcctgggttc gggccctggc cacccaggtg cagaagatcg aggacccgag cggcacgggg
      721 gctcccgggg tgccccaggt gctccccgtc gagtccgcgg ccgtgcccac cgcacccgcc
      781 tccccgaacc ccaggctcaa cctcatgctc ggagcggtgc tggggctcgg gttggcgctc
      841 ggctacgcga tgctgcgcca caccgtcgac cgcaggctgc gcagcaagga ggagatcgag
      901 gcccagttcc agatcagcgt ggtgggcggt gtgcccgtct cccaggtgct caagaaggac
      961 gcccgtggtg ggcacgggca cctcgccgtc gacgccgcgg tctcggcagc caacggcccg
     1021 gcatccgagg cgttccgcaa gctacgcacc aacctcatgt acatgaacgt cgacaacccc
     1081 ccgcgcgtcg tggtggtcac cagccccctg cccggcgacg gcaagtcgac ggtggccgcc
     1141 aacctcgccg cggccatcga ctcctcgggc gagaacgtgg tgctcatcga cggcgacctg
     1201 cgccgcccga ccgtggccac ctggttcggc ctggtcgagg gggtcgggct caccgacgtg
     1261 ctggccgggc ggatccccgt cgaggacgcc ctgcagacgc ccgtggagca ccccaacctg
     1321 cgcgtgctcg gagccggggc catcccgccc aacccgagcg agctgctcgg ctcgcaggtg
     1381 atgcgccagc tgctgcagaa gctgtcggcc gacgcgttgg tgctcatcga cgcgccaccc
     1441 ctgctgcccg tcaccgacgc cgcgttgctg acggccaacg ccgacggcgc cctcgtggtg
     1501 gtgtcggcgg gcaagaccct ggacacccag ctcggctccg ccctggacca cctctcggac
     1561 gtcgaggcca aggcgctggg cgtggtcctg aaccgggtct cgccgcgtga cgccggcaac
     1621 ggctactacg accagtacag cggctactac acgcagcccg gccggaggcg cagcaagaag
     1681 tgacccacac gggcgaggcc gtgtggcggc acaggggcga ggtcgcggcg gtcgactcgg
     1741 gcgaccgcgt cgccgtgctc gacctgacca gcctcgccgt cccacctcgg atcctcgagg
     1801 ggccggctgc ggcgatctgg cgggccgtcg acggtgaacg tgcgaccccc gaggtggtgc
     1861 aggccgtggc gcagtcgttc ggggtggagg cggctgagat cgaggcggac gtgctggcgt
     1921 tcctggcgtc gctgtcctcc gccgggttgc tcgaggcctc gtgatggtcg acaccgcgct
     1981 gcggctgcgc gctgacgagg ccgtgcctct gctgcacacc ctggtggctc gggccgccgc
     2041 cgacctgggg ctgcgggtgc tggcgatcaa ggggccggtc gcggccctgc aggggttgcg
     2101 gaagccgcgc ccctctgcgg acctggacgt gctggtgcac cctcgtgacc tcgagtcgtt
     2161 ggtggccggc ctggtcgccc tcggctggcg acgcggggtc gagggcacct acgcgagcat
     2221 catgcccccg cactcggtga acctcctcag cgacctgtgg ccggtcgcca tggacgtcca
     2281 ccactacttc ccgggcttcc tcgccgacgc gggcgcggtg ttcgacgtgc tgtgggcagg
     2341 gcacgagctg gtgacgctgg ccggggtgcc ggtgccggcg tgcgacccgg tcggacaggt
     2401 cgctgtcctg gcgctgcacc acctgcgcat cagccccgac ggagacagcc cggcgctgcg
     2461 ggagctgtct tcgcgcgccg gtgcgacgct ctccgcggac gaggtgcgcg cgctcgtgca
     2521 gctggcccac gcgacggatg cgaccgccac cctgcgaccg ttcctcctgg ccctgggggt
     2581 gccggatgcg gagatcgggc cggcacggga cgccatcgct ctcgacgcat ggaaccggcg
     2641 ggtccgggcg gtcgggtacg aggcgtggtt cctgaagttc gcgcggacgc ccaagcgccg
     2701 atggccacgc accctgtggc acgcgctggt gctcaccgac gacgagatcc acgtgtacag
     2761 caacgtcccg ccgggctcgg gggggctcct gcggctgcgc ctgcgccgaa tcggccgcgg
     2821 cctggccggc gtgccgagga cggttcgggc gatggcccgg gagcggcgac ggggcagcgg
     2881 gtagggcgtc agcgcgtggt ctcgtcgacc agcgccggga ccacgatcgt gaggagctcg
     2941 tcgatctggg cggcgctgcg ggcgttcgcg gcgctgccgc gccggtaggg gtctcggacg
     3001 tcgtgctcgg ggcgtgccgg gggttgccga agccccaccg ccgtgatcag ctcccgcccc
     3061 ctcagctcgg tgtgcccggc cgcctcggcg aactggccca gggtgagcac cttgcggaag
     3121 gcatgcgggt actcctcgag caggaaggtc cggtgcgacg cctccgcggt gaggacgagg
     3181 tccgcgtcgt cgatgaggtc gcgggtgacc ctcctgctcg cgaagccgtc gtgagcgcct
     3241 ccctcgggga ggaagcgcgc catctcggcg tccatcggct gggacacgta gccgtgggtc
     3301 ccggcgctgg agaagacgac gtcggtgcgc gacccggcca ggtggcgggc agcggcctct
     3361 gcgtaggccg agcggcagat gttggccgtg cacacgaaga gcacggtgat ggggaccctg
     3421 ggcacccggg gagggtagcg cgcttccatc tgggatgatg tcgcccatga ccgagcccct
     3481 ttcgccatat cgtgctgcct ccgtgccgga caagccgtcc ctcgacggcc t
//
LOCUS       DAOSOC010000217         3505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_26868_length_3505_cov_9.038551, whole genome shotgun sequence.
ACCESSION   DAOSOC010000217 DAOSOC010000000
VERSION     DAOSOC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26868_length_3505_cov_9.038551"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PL014_12015"
     CDS             <1..891
                     /locus_tag="PL014_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT57862.1"
                     /translation="TVHGVQRVDNATIVYWSLGIPEGSQAEFAASYFGPSTLSFRSKR
                     TGVNQSDVTLTDGAAGLTYRPMEPAGKFRTCVCGPSTTMTDLTPGQAQIIWSAVAPLP
                     AGVATVDVTIAEQVIPNVLVGDGPLLPLAENQEAPFVPGMGWPEPDPELLATARTSQP
                     AAFELTQRVSNVEQSVTTSTGEVALAADVLFAKNSATLTAKGTKTVADAAAQIKVTET
                     GKALTVTGHADSDGSNSYNQSLSEKRAKAVATALTKALGGGYTITAVGKGETEPIASN
                     KTTAGKAKNRRVSITFTEGQ"
     gene            894..1469
                     /locus_tag="PL014_12020"
     CDS             894..1469
                     /locus_tag="PL014_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57863.1"
                     /translation="MAQLPRRPRLTLALLGVVSVVALSSCTGDDPEPTASPTGSASSS
                     ASTAPSTPLRDQSSLVLGAQLPPVAGSSTGDVAGAPATLNVATVVATQGGTVLTFWHT
                     GTEKMQVNAGDLSWEAQPVLVDVGEQKVYEPVTFVDEQGDTRCLCTDAAYIGGVPQPR
                     TIFFPQLPDTVTTVEVRQAGFDKPISVPVTR"
     gene            1521..2588
                     /locus_tag="PL014_12025"
     CDS             1521..2588
                     /locus_tag="PL014_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT57864.1"
                     /translation="MTTIRSSLRSIGAATAVGAVLLVGMPAGSSAAGTSAADEVPILG
                     QAFTTGTVQSGPPAVVTVHGVRRVEGATILYWSLGVPVDSPVDNALSFLGPATSSFYA
                     GNVGPTMGDAALTDVTGAQVYRPLVTTEKFAPCACSPVSSLLKVKPGQASVMWTALAP
                     LPEGVTTVDVTVAEQVVPDVTVEEGLLLPVASEQEPVIVGMGWPEVDMNLVGTSTPQD
                     PESYSLVGRVSDLERAVTTSQGEVSLASDVLFAKNSATLTAKGVATVAKAAAQIKASE
                     AGTALTVTGHADSDDSEDYNQALSERRAKAVAAALTKELGSGYSITAVGKGETQPIAS
                     NATAAGKAKNRRVSITYTQGQ"
     gene            2627..3178
                     /locus_tag="PL014_12030"
     CDS             2627..3178
                     /locus_tag="PL014_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57865.1"
                     /translation="MALASIAVAAIAALAACTDDTPEPSPSGSGSSSATASSAPAFVP
                     SDQSAKVLAATLPAVQGSAKGTVGKQPATLNVAEVRATAGGTLLTFWFSGAQAAPLGD
                     QGEKSWENQPTLVDVAGKKVYEPFTFTHSRGETYCLCTDAAYARGDAQPRTIYYPALP
                     DTVTTVEVRQAGFDKPISVPVTR"
     gene            complement(3252..>3505)
                     /locus_tag="PL014_12035"
     CDS             complement(3252..>3505)
                     /locus_tag="PL014_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57866.1"
                     /translation="LDLRLGPCVLPDAPEPPEPEPTPTPTPSSTATPTPTPTPTPDDV
                     VKKGRCGDVEFDVTFPGSGGPPSFGWGQLKIEADPRLTD"
BASE COUNT          556 a         1273 c         1172 g          504 t
ORIGIN      
        1 acggtgcacg gggtccaacg ggtcgacaac gccacgatcg tctactggtc actcggcatc
       61 cccgaaggtt cgcaggcgga gttcgccgcg tcctacttcg gtccgtccac gctgtcgttc
      121 aggtcgaagc gaacgggtgt caaccagtcg gacgtgacgc tgaccgacgg ggccgctggt
      181 ctcacctacc gcccgatgga accggccggc aagttcagga cctgcgtgtg cggcccgtcc
      241 accaccatga ctgatctcac tccggggcag gcccagatca tctggtccgc cgtggctccc
      301 ctgccagcgg gcgtcgcgac ggtcgacgtc accatcgcgg agcaggtgat cccgaacgtc
      361 ctcgtgggag acggccccct gctgcccttg gcggagaacc aggaggcgcc gttcgtcccc
      421 ggcatggggt ggcccgagcc cgaccccgag ctattggcca cggcgcgcac ctcgcagccc
      481 gcggccttcg agctcaccca gcgcgtgtcc aacgtcgagc agagcgtcac gacctcgacc
      541 ggcgaggtcg ccttggccgc ggacgtgctg ttcgccaaga actccgcgac cctgacggcc
      601 aagggcacga agacggtcgc cgatgccgcc gcgcagatca aggtaaccga gaccggcaag
      661 gccctcaccg tgacgggtca cgcggactcc gacggctcga actcctacaa ccagtcattg
      721 tcggagaagc gggcgaaggc cgtcgcgacg gcgctcacca aggcgctcgg cggtgggtac
      781 accatcacgg ccgtcggcaa gggcgagacc gaaccgatcg cgtcgaacaa gaccactgcg
      841 ggcaaggcca agaaccgccg agtcagcatc accttcaccg aaggacagtg accgtggccc
      901 agcttccccg tcgtccccgt ctcactctgg cgctcctcgg tgtggtgtcc gtcgtggcgc
      961 tgtcgtcctg cacgggggat gatccggagc ccacggcgag cccgaccgga tcagcctcgt
     1021 cgagcgcgtc cacggcgccg tcgacgccgc tgcgcgacca gagttctctc gtgctcggag
     1081 cgcagcttcc cccggtggcc ggcagctcga ccggggacgt ggccggcgcg ccggccaccc
     1141 tcaacgtggc caccgtcgtc gccacccagg gcggcaccgt cctgaccttc tggcacaccg
     1201 gcacggagaa gatgcaggtc aacgccgggg acctctcctg ggaggcccaa cccgtgctgg
     1261 tcgacgtcgg ggagcagaag gtctacgagc ccgtcacgtt cgtggatgag cagggggaca
     1321 cccgctgcct gtgcacggac gcggcgtaca tcggtggagt gccgcaaccg cgcacgatct
     1381 tcttcccaca gctgcccgac acggtcacca ccgtcgaggt ccgtcaggca ggcttcgaca
     1441 agccgatctc ggtacccgtc acgcggtgac cggcgcggcc accgagagtg ttgccgaaac
     1501 catctggggg gaaccacgtc gtgacgacca ttcgctcaag cctgcgctcg atcggggccg
     1561 ccactgcggt gggggccgtg ctcctcgtgg ggatgcccgc cgggtcgagt gccgcgggga
     1621 catccgccgc cgacgaggtc ccgatcctcg gccaggcctt caccacgggg acggtgcagt
     1681 ccggacctcc cgccgtcgtg acggtccacg gcgtccgccg ggtggagggg gccaccatcc
     1741 tgtactggtc cctgggcgtc cccgtcgaca gcccggtgga caacgcgttg tcgttcctcg
     1801 gccccgcgac gtcctcgttc tacgcaggca atgtcgggcc gacgatgggc gacgccgctc
     1861 tcaccgacgt cactggcgcg caggtctacc gcccccttgt caccaccgag aagttcgcac
     1921 cgtgtgcgtg ctcgcccgtg tcgtccctgc tcaaggtcaa gcccggtcag gcctccgtca
     1981 tgtggacggc cctcgcgccg ctccccgagg gcgtcacgac agtcgacgtg accgtcgctg
     2041 aacaggtggt cccggacgtc accgtcgagg agggcctgct cctgcccgtg gccagcgagc
     2101 aggaacccgt catcgtcggc atgggttggc ccgaggtgga catgaacctc gtcggcacgt
     2161 cgacccccca ggacccggag tcctactccc tcgtcgggcg cgtctccgac ctcgagaggg
     2221 ccgtgacgac ctcgcagggt gaggtgtccc tggcctccga cgtgttgttc gcgaagaatt
     2281 ccgcgacgct gaccgccaag ggtgtcgcca cggtggccaa ggccgcggcg cagatcaagg
     2341 ccagtgaggc cggcacggcc ctgaccgtca ccggccacgc cgactccgac gactccgagg
     2401 actacaacca ggcgctttcc gaacggcggg ccaaggcggt ggccgcggca ctgacgaagg
     2461 agttgggctc cgggtactcg atcacggcag tcgggaaggg cgagacccag ccgatcgcct
     2521 cgaacgcgac agctgcgggc aaggcgaaga accgacgcgt cagcatcacc tacacgcagg
     2581 ggcagtgatg aacaccgcta ccgcagccac gagggttcgc tcgcgggtgg ctctggcctc
     2641 gatcgccgtc gccgcgatcg cagccctcgc agcctgcacc gacgacaccc ccgagccctc
     2701 gcccagcggc tccggcagtt cgtcggcgac ggcctcgtcc gcgccggcct tcgttcccag
     2761 tgaccagagc gcgaaggtgc tcgcggcgac cctgcctgcg gtgcagggca gcgccaaggg
     2821 cactgtcggc aagcagcctg ccaccctgaa cgtcgccgag gtgagggcga ccgccggggg
     2881 aaccctgctt accttttggt tctccggcgc gcaggcggcg ccactcggtg accagggtga
     2941 gaagtcctgg gagaaccagc ccaccctcgt cgacgtcgcc gggaagaagg tctacgagcc
     3001 gttcaccttc acccacagcc gcggcgagac ctactgcctg tgcactgatg ccgcctacgc
     3061 ccgcggggac gcgcagccgc gcaccatcta ctacccggcg ctgcccgaca cggtcaccac
     3121 cgtcgaggtt cgccaggccg gcttcgacaa gccgatcagc gtcccggtca cccgctgacc
     3181 cgacagacac ggaatcggcg cagggaggcg acgtcgcctc cctgcgccga ttccgtcgac
     3241 gtcgatggcc cctagtcggt gagacgaggg tcggcctcga tcttcagctg cccccaaccg
     3301 aagctgggtg gcccaccgct gcccgggaag gtgacgtcga actccacatc gccgcaccgg
     3361 cccttcttca ccacgtcgtc aggtgtcgga gtgggcgtcg gcgtgggggt cgccgtcgac
     3421 gacggggtcg gcgtgggagt cggctcgggc tcgggcggct ctggcgcatc cgggaggacg
     3481 cagggcccca gccggaggtc gagct
//
LOCUS       DAOSOC010000218         3501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_26914_length_3501_cov_9.552815, whole genome shotgun sequence.
ACCESSION   DAOSOC010000218 DAOSOC010000000
VERSION     DAOSOC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26914_length_3501_cov_9.552815"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..332
                     /locus_tag="PL014_12040"
                     /pseudo
     CDS             1..332
                     /locus_tag="PL014_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            641..1240
                     /locus_tag="PL014_12045"
     CDS             641..1240
                     /locus_tag="PL014_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57867.1"
                     /translation="MTNPDIRERFARYLAEQSNRGLGSIPLAPTRALAPISRGLMAGA
                     TNLLTKALRALEADDEARARQFVERAAALPYDDHEERHPGVAMAQQELFTEIVDILED
                     CEDHWVAAVADVFAAAQEQARFALRDVLNDILQDFSLTDDEQRRVRHLIRDIPQRAEL
                     CDLDLDLQARASAIFEILEAVIIFRDALDEIHALEDDEE"
     gene            complement(1366..2952)
                     /locus_tag="PL014_12050"
     CDS             complement(1366..2952)
                     /locus_tag="PL014_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT57868.1"
                     /translation="MKRTVGTAGVFGVLLGAALVVAPSSGAAPAQNGCDNRTNNTYQK
                     LLECVRLNGVVEHMEKLQAIADANGGNRADQTSGYQASVDYVVKTLEDAGWSADVVPF
                     TYDAADVILKQLTPVAANYIALDAAGTGEGDVTGSVIPVDINLAGNRANSSGCEAADF
                     AGLDFSGSNDIALLQRGTCSFSVKAQNAQDAGAEAVVLFNQGDPGAGDRFGPLNPTLA
                     PLTLDIPVVGTTFAAGEALAAPGATAQVKVDFYDAESFNVIGELAGKTNDNVVMAGAH
                     LDSVPAGPGINDNGSGSAALLEVAQQMAKSKPQNTVRFAWWGAEELGLIGSTEWVGQR
                     TPEELDQIALYLNFDMIGSPNYYFGVYDANESSFAAPVAVPDGSIDIEKTFESFYTLR
                     GEPYDDSAFSGRSDYAAFIDNGIPSGGLFTGAEERKTAQQQALWGGTTGAQFDPCYHL
                     ACDTIDNYDAHALEVNADAVAYSVFTYAASTEGVNGVRGAKVPGNFSVPAPAGPEHTF
                     AGPLGGADHGHGHDHDRVSE"
     gene            complement(3185..>3501)
                     /locus_tag="PL014_12055"
     CDS             complement(3185..>3501)
                     /locus_tag="PL014_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT57869.1"
                     /translation="VLADELKGIVERWRLELTVRRDSVAWRILPILLAQPAVTSRLVQ
                     ERTGVSQPAADRALRQLGEAGILTSKNEHGRDRKRDVVWRSDEVITALDEFAARARRA
                     RP"
BASE COUNT          591 a         1229 c         1135 g          546 t
ORIGIN      
        1 ccgaccccga agtccagcga cggacccgtg gagtcgatcc gggtgctggc gatggtgcgc
       61 gacaccggat caccggcaaa ccagccttcg ccgcgctcac cggagtcagc ccgatcccgg
      121 cgtccagcgg caagaccaac cggcaccggc tcaaccgcgg cggcgaccgc cgagcgaaca
      181 acgcgatcca caccatcgcc ctggtccgga tgacgatgga cccccgcatc aaggcctaca
      241 tcgcgaagaa gaccgccgag ggcaaactca aacgcgaagc gattcgctgc ctcaaacgcc
      301 acatcgccaa cgaggtcctc gccctgctga ccaacccacc aaccgtcccc gacgtcagcg
      361 acctgcgacc agcacgacaa gcccgtggcc tatccctcca gaccgtcgcc gaccacttcg
      421 gcatctggcc catgcacatc tccaccgtcg aacgcggcaa acgccgcgac gacgacttca
      481 cccgccgcta ccgcgaatgg ctcaacgccg cttgacaacc cataggagca tcacggcccc
      541 gaagttttcc gcagggccac cgagcagtga tcaccgcggc tgctcgacga tgggccagac
      601 caactgccaa cacacgtcac cgagcgggta ccttgcctga gtgaccaacc ccgacatccg
      661 cgagagattc gcccgctacc tggccgaaca gtcgaaccgc ggtctgggca gcatccccct
      721 cgccccgacc agggccctcg caccgatcag ccgcgggctg atggccggcg cgacgaacct
      781 gctcaccaag gcgctgcgag cgctcgaagc cgacgatgaa gcccgcgcac gacagttcgt
      841 cgagcgagcc gccgcgttgc cctacgacga ccacgaggag cgtcaccccg gcgtcgccat
      901 ggctcaacag gagctgttca cagagatcgt cgacatcctt gaggactgcg aggaccactg
      961 ggtcgcggcc gtcgctgacg tcttcgccgc cgcgcaggag caggccaggt tcgccctgcg
     1021 cgacgtcctg aacgacatcc tccaggactt cagcctgacc gacgacgagc agcgtcgcgt
     1081 ccggcacctc attcgcgaca tccctcagcg ggccgagctg tgtgacctcg acctcgacct
     1141 ccaggcccgg gcgtcggcga tcttcgagat ccttgaggcc gtgatcatct tccgagatgc
     1201 tctcgacgag atccacgcac tggaggacga cgaggagtaa cgacgccgga tgccggccac
     1261 acggcatccg gtgtgatctc ccgtcccagc cgctttcgag gcagagcgaa ggggtgggtg
     1321 gacgtcaccg tccacccacc ccttgccgag tcacgtcagg tcagatcact cgctgacgcg
     1381 gtcatggtca tggccgtgac cgtggtcagc gccacccagc gggccggcga acgtgtgctc
     1441 cgggccagcc ggcgccggga cgctgaagtt cccgggcacc ttcgccccac gcacaccgtt
     1501 gacgccctcg gtggaggcgg cgtacgtgaa gaccgagtac gccacggcat ccgcgttgac
     1561 ctccaacgcg tgagcgtcgt agttgtcgat cgtgtcgcag gcgaggtggt agcacgggtc
     1621 gaactgcgcg cccgtggtgc cgccccagag ggcctgctgc tgagccgtct tgcgctcctc
     1681 ggcgccggtg aacaggccac cggaggggat gccgttgtcg atgaacgcgg cgtagtcgga
     1741 gcgaccgctg aacgccgagt cgtcgtaggg ctcgccacgc agggtgtaga acgactcgaa
     1801 ggtcttctcg atgtcgatcg agccgtccgg aaccgcgacc ggcgccgcga agctcgactc
     1861 gttggcgtcg tagaccccga agtagtagtt cggcgagccg atcatgtcga agttgaggta
     1921 gagcgcgatc tggtcgagct cctccggcgt gcgctgaccg acccattcag tcgagccgat
     1981 gaggccgagc tcctcggcac cccaccacgc gaaccgcacg gtgttctggg gcttggactt
     2041 ggccatctgc tgcgcgacct cgagcagggc ggccgaaccg ctgccgttgt cgttgatgcc
     2101 cgggcccgcc ggcacggagt cgaggtgggc accggccatg accacattgt cattggtctt
     2161 gccggcgagc tcaccgatga cgttgaacga ctcggcgtcg tagaagtcga ccttgacctg
     2221 tgccgtggca cccggcgcag cgagcgcctc gccggccgcg aaggtcgtgc cgacgacggg
     2281 gatgtccagc gtcaacggtg cgagggtcgg gttcagcggg ccgaaccggt cgccagcgcc
     2341 cgggtcaccc tggttgaaca gcacgacggc ctcggcgccg gcgtcctggg cgttctgggc
     2401 cttgaccgag aacgagcagg tgccacgctg aagcagcgcg atgtcgttcg agccggagaa
     2461 gtccagaccg gcgaagtcgg cggcctcgca gccgctgctg ttggcccggt tgccggcgag
     2521 gttgatgtcg acggggatca cgctgcccgt cacgtctccc tcgcccgtcc cggcggcgtc
     2581 gagggcgatg tagttcgccg cgaccggggt gagctgcttg aggatgacgt cggccgcgtc
     2641 gtaggtgaac ggcacgacgt cagccgacca accggcatcc tcgagcgtct tgacgacgta
     2701 gtcgacactg gcctggtagc ccgaggtctg gtcggcgcgg ttgccgccgt tggcgtcggc
     2761 gatcgcctgc agcttctcca tgtgctcgac gacaccgttg agccgcacgc actccaacag
     2821 cttctggtag gtgttgttgg tgcggttgtc gcagccgttc tgggccggtg cggcacccga
     2881 ggacggtgcg acgaccaggg ccgcgcccag caggacgccg aacacccctg ccgttcccac
     2941 cgtgcgcttc atgcgcggac ttgtcatcag gactcctatg tcactggtta cgaggagggc
     3001 gtcgtcatgc agacaccctg gccggtgacc gtaactgccg atcggcccga tttggccggt
     3061 acgtgcacac caattgacgt gggctctcac cgggcgtcgt cgtggcactc gccgcaaacc
     3121 ctcggatgcc gtccgcgccg ttcgcgttct cccccaccgt ggtccgtgat gccacccacg
     3181 tcactcaggg ccgggcacga cgggcacgtg cggcgaactc gtccagggcg gtgatcacct
     3241 cgtcggatcg ccagacaacg tcacgcttgc ggtcgcggcc gtgctcgttc ttggaggtga
     3301 ggatgccggc ctcgcccagt tgccgcagtg cacggtcggc tgcgggttgg gagacgccgg
     3361 tgcgctcctg caccagtcgg gaggtcactg ccggctgggc gagcaggatc ggcaagatcc
     3421 tccacgccac cgagtctctg cgcaccgtga gctcgagacg ccagcgttcg acgatgccct
     3481 tcagttcgtc ggccaggact c
//
LOCUS       DAOSOC010000219         3485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_27071_length_3485_cov_13.342274, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000219 DAOSOC010000000
VERSION     DAOSOC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27071_length_3485_cov_13.342274"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="PL014_12060"
     CDS             <1..1395
                     /locus_tag="PL014_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57870.1"
                     /translation="LISRSTNDVTQVQTVVYMMLAMMVSAPIMMVGGVVMAIREDAGL
                     SWLVAVAVPALAIAIGLVIRQMIPHFRAMQTAVDSVNRILREQITGIRVVRAFVREDV
                     ERERFAEANTTYTGTALAVGKLMALAFPIVMVIFNASTVAVLWFGAQRIDTGAMQIGA
                     LTAFMAYLIQILMSVMMATFMAMMIPRATVSAGRIQEVLDTQTSVHAPADPVAIPAGP
                     VRVELRDVTFAYPGAEAPVLHEVSLVAEPGTTTAVVGSTGSGKTTLLGVVPRLHDVTS
                     GQVLVGGVDVRDADPEHLWSHIGLVPQRPYLFTGTVASNLRYGDPDATDDELWEALRV
                     AQAEDFVREMPDGLESPISQGGTNVSGGQRQRLAIARALVSKADIYLFDDAFSALDVA
                     TDARLRAALRPVTRDAAVIVVAQRVSTILHADRIVVLDEGRVVGSGTHEELLETCPTY
                     LEIVQSQQAIEEAA"
     gene            1392..3407
                     /locus_tag="PL014_12065"
     CDS             1392..3407
                     /locus_tag="PL014_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57871.1"
                     /translation="MSEVRNEEEKAAAQRRGPASAHRGGPPHAAIGMPTEKSQNFLPS
                     AKRVLALMAPERLIVWAVVLLALGSVALNAIGPKILGSATDLIFAGVFSRQIPAGVTQ
                     EQAVQALRARGETQIADMLAAMQDIVPGTGIDFGALGRILLIVLAIYVVAALLQWLQG
                     RLLTGAVNRTIFTLRRDVEDKLNRLPLPYVDGQPRGELLSRVTNDIDNVAQSLQQTLS
                     QLLTSLLTVVAMVGMMIWISPLLALIALVTIPVTMVVTAMIGKRSQKHFVQQWKSTGE
                     VNAIVEESFTGHELVKVFGRQAEADRDFAVRNDALHHASFRAQFISGIIMPTMMFIGN
                     LNYVAIAVVGGLRVASGSMSLGDVQAFIQYSRQFTQPLTQVASMANLMQSGVASAERV
                     FAVLDAEEQEPDAVTPARITDPRGRVAFEDVSFSYREDTPLIENLDLVVEPGQTVAIV
                     GPTGAGKTTLVNLVLRFYELNGGRITLDGVDITELTRHDLRSRIGMVLQDTWLFSGTI
                     RENIAYGRPGATDAEVLEAARATYVDRFVHMLPDGYDTVLNAEADNISAGERQLITIA
                     RAFLAAPSLLILDEATSSVDTRTELLVQHAMSALRSDRTSFVIAHRLSTIRDADLILV
                     MESGRIVERGSHAELLAAGGAFAALYAAQFAGAATDLDEESGIPAVV"
BASE COUNT          524 a         1236 c         1184 g          541 t
ORIGIN      
        1 ctcatcagcc gcagcaccaa cgacgtgacc caggtgcaga ccgtcgtcta catgatgctg
       61 gcgatgatgg tgtccgcgcc gatcatgatg gtcggcggcg tcgtcatggc cattcgtgag
      121 gatgccgggt tgtcctggct cgtcgccgtc gccgtcccag cgttggccat cgccatcggg
      181 ctcgtcatcc ggcagatgat cccgcacttc cgggccatgc agacggccgt ggactcggtg
      241 aaccggatcc tgcgcgagca gatcaccggc atccgggtcg tgcgggcgtt cgtgcgtgag
      301 gacgtcgagc gcgagcggtt cgccgaggcg aacaccacct acaccggcac ggcgctggcc
      361 gtcggcaagc tgatggccct ggccttcccg atcgtcatgg tcatcttcaa cgcctccacg
      421 gtcgcggtgc tgtggttcgg ggcgcagcgc atcgacaccg gggccatgca gatcggcgcg
      481 ttgaccgcgt tcatggctta cctcatccag atcctcatgt cggtgatgat ggcgaccttc
      541 atggcgatga tgatcccgcg ggccacggtg tcggcggggc gcatccagga ggtgctcgac
      601 acccagacct cggtgcacgc gccggccgac ccggtggcga tccccgcggg gccggtacgg
      661 gtcgagctgc gcgacgtcac cttcgcctac ccgggcgccg aggcccccgt gctgcacgag
      721 gtctcgctcg tcgccgagcc gggcacgacg accgcggtcg tcgggtccac gggttcgggc
      781 aagacgacgc tgctcggggt cgtgccgcgc ctgcacgacg tgacctcggg gcaggtgctc
      841 gtcggtgggg tcgacgtgcg cgacgccgac cccgaacacc tgtggtcgca catcgggctc
      901 gtcccgcagc ggccctacct gttcaccggc accgtcgcct ccaacctgcg ctacggcgac
      961 ccggacgcga ccgacgacga gctgtgggag gccctgcgcg tcgcccaggc cgaggacttc
     1021 gtgcgggaga tgcccgacgg cctcgagtcc ccgatctcgc agggcggcac caacgtcagc
     1081 ggcggtcagc ggcagcggct cgcgatcgcc cgtgccctgg tgagcaaggc cgacatctac
     1141 ctcttcgacg acgccttctc ggctctggac gtcgcgaccg acgcccggtt gcgcgccgcg
     1201 ctgcgcccgg tcacccgcga cgccgcggtg atcgtcgtgg cgcagcgggt gtccacgatc
     1261 ctgcacgccg accgcatcgt cgtcctcgac gaggggcggg tcgtcggcag cggcacccac
     1321 gaggaactgc tcgagacctg cccgacctac ctcgagatcg tccaatccca gcaagcgatc
     1381 gaggaggcgg catgagtgag gtgcgcaacg aggaggagaa ggcggccgca cagcggcgcg
     1441 gcccggcatc cgcccaccgt ggtgggccgc cgcacgcggc gatcgggatg ccgaccgaga
     1501 agtcgcagaa cttcctcccg tcggccaagc gggtcctcgc gctcatggcg cccgagcggc
     1561 tcatcgtgtg ggccgtcgtg ctgctggcgc tcggcagcgt cgccctcaac gcgatcggcc
     1621 cgaagatcct cggttcggcc accgacctga tcttcgcggg cgtgttcagc cgccagatcc
     1681 cggccggggt cacccaggag caggcggtgc aggcgctgcg ggcccgcggc gagacgcaga
     1741 tcgccgacat gctcgcggcg atgcaggaca tcgtgccggg cacgggcatc gacttcggcg
     1801 ccctcgggcg catcctgctc atcgtgctcg cgatctacgt ggtcgccgcg ctgctccagt
     1861 ggctccaggg gcggctgctc accggagcgg tcaaccgcac gatcttcacg ctgcgtcgcg
     1921 acgtcgagga caagctgaac cggctgcccc tgccctacgt cgacgggcag ccgcgcggtg
     1981 agctgctcag ccgggtcacc aacgacatcg acaacgtcgc gcagagcctg cagcagaccc
     2041 tgagccagct gctcacctca ctgctgaccg tcgtcgccat ggtcggcatg atgatctgga
     2101 tctcgccgct gctcgccctc atcgcgctcg tgacgatccc ggtgacgatg gtcgtcacgg
     2161 cgatgatcgg caagcgcagc cagaagcact tcgtgcagca gtggaagagc accggcgagg
     2221 tcaacgccat cgtggaggag tccttcaccg ggcacgagct cgtcaaggtc ttcggccgcc
     2281 aggccgaggc cgaccgcgac ttcgccgtgc gcaacgacgc cctgcaccac gcgagcttcc
     2341 gggcgcagtt catcagcggc atcatcatgc cgacgatgat gttcatcggg aacctcaact
     2401 acgtggccat cgcggtcgtc ggtgggctgc gggtcgcgtc gggctcgatg agcctgggtg
     2461 acgtgcaggc gttcatccag tactcgcgcc agttcaccca gccgctgacg caggtggcgt
     2521 cgatggccaa cctcatgcag agcggcgtcg cctccgccga gcgggtgttc gcggtgctgg
     2581 atgccgagga gcaggagccg gatgccgtca ccccggcacg catcaccgac cctcgcgggc
     2641 gggtcgcgtt cgaggacgtg tcgttctcgt accgggagga caccccgctc atcgagaacc
     2701 tcgacctcgt cgtcgagccg ggccagacgg tggcgatcgt cggcccgacc ggggcgggca
     2761 agacgacgct ggtcaacctc gtgctgcgct tctacgagct gaacgggggt cggatcaccc
     2821 tcgacggggt cgacatcacc gagctgaccc gacacgacct gcgttcacgc atcggcatgg
     2881 tgcttcagga cacctggctg ttctccggca cgatccgcga gaacatcgcc tacgggcggc
     2941 cgggggcgac cgacgccgag gtgctcgagg ctgcccgggc cacctacgtc gaccggttcg
     3001 tgcacatgct gcccgacggc tacgacaccg tgctcaacgc cgaggccgac aacatcagcg
     3061 ccggtgaacg acagctcatc accatcgccc gggccttcct cgcggcgccg tcgctgctca
     3121 tcctcgacga ggcgacgtcg tcggtggaca cccgcaccga gctgctcgtg cagcacgcga
     3181 tgtctgcgct gcggtcggac cgcacgagct tcgtcatcgc ccaccgcctc tcgacgatca
     3241 gggatgccga cctcatcctc gtcatggagt ccgggcggat cgtcgagcgg ggttcacacg
     3301 ccgaactgct cgccgctggt ggcgcgttcg ccgccttgta cgcagcccag ttcgctggtg
     3361 cggcgaccga tctcgacgag gagagcggca tccccgccgt tgtctgagga gggtcaggcc
     3421 gggtggttct cgcgccacca gtccatggcg accacggcgc ccacagccgg taccgcgggc
     3481 agcag
//
LOCUS       DAOSOC010000220         3476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_27149_length_3476_cov_7.933061, whole genome shotgun sequence.
ACCESSION   DAOSOC010000220 DAOSOC010000000
VERSION     DAOSOC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27149_length_3476_cov_7.933061"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PL014_12070"
     CDS             complement(<1..350)
                     /locus_tag="PL014_12070"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOT57872.1"
                     /translation="MGGRVAIAATGPVSLAAGREVALAGGNAVDVAVAAAMAAMSTEP
                     GIVSLAGGAFISVWPADGDPVVIDGNVEMPGRGLPADRFGGGVDEVSFGYGGGVTLFG
                     GHGSVATPGSVTAC"
     gene            454..1230
                     /locus_tag="PL014_12075"
     CDS             454..1230
                     /locus_tag="PL014_12075"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HOT57873.1"
                     /translation="MTRSPLAIIAGTGFYDLDALENPTQETVDTVWGQARVTEGEWHG
                     IPVVFLTRHGAGHSVPPHLVNYRANIRALADLGVRDVVAVNVTGSIDPTLQPGDLLCL
                     DDFIDFTKQRPVTFFDGSTPEGVVHTDVMTPYHPAIRREILDAAATAGHTIRDGGVYA
                     CFEGPRFETRAEIRLAALAGADVAGMTGVPEVTLAVEAGLRYAAISLVVNPASGVGDA
                     DEPIAMDEINAVLAASSATVLEILDDLVHTRATFPEEPDA"
     assembly_gap    1331..1430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1431..2630
                     /locus_tag="PL014_12080"
     CDS             <1431..2630
                     /locus_tag="PL014_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT57874.1"
                     /translation="RGKLVMPGLVNLHTHLPMTLLRGLAEDVDLDGFLARVWAAEGAV
                     MDTDTVELGATLGALESLRGGCTTQLDMYFHHEATHRGAVAAGSRHVIGPVFFDGPGP
                     DGLTWEQRLDNLRSWPAVLDEIGGPSVPVGAMPHATYTCSPEGFAQVVTVLRELVANS
                     ERDGILTTHVSETAAENAGIRERYDATPTELLARAGWVEPDLPLVIGHGVHLSEGDRT
                     TLAAGGAAVGHCPGSNLKLASGALPWQVLQDSGLRVGLGTDGCSSSNDLDMWQAMRQA
                     ALLARLTSERPDVASAHEVLRAATIEGARALGMGDVIGSVEVGKRADLVLVDLEAPHL
                     TPVHDVAALLVFAAGRGDVTDVLVDGMPVVRDRRSTRLDTGQLLERARVRGEVARAAA
                     AEAAAGR"
     gene            2627..3094
                     /locus_tag="PL014_12085"
     CDS             2627..3094
                     /locus_tag="PL014_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT57875.1"
                     /translation="MTSESADEVIARLGMLPIPEEGAWFAPGPRTHGLNGIIALLTDR
                     PDGFSAMHRLTIDEGWQWMAGAPATLVRLHPDGTGDSTTLGPDSPQALVTKGSWMGAR
                     THGEWTLFSCWCAPAFEPEHFELGERSALLDAFPDHAAEITALTRTHPIGQMR"
     gene            3091..>3476
                     /locus_tag="PL014_12090"
     CDS             3091..>3476
                     /locus_tag="PL014_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT57876.1"
                     /translation="MTALVTGASGGLGRGIAVALAAAGHDIGVHFRSDAVGAAAAVTD
                     VEAVGRRATALHADLAVDDPAELDRACDDLLDACTAALGAPEVVVLNAFPQHVVAWGD
                     LDTAAWDTYHRGGLRPTTSLLHRAAAR"
BASE COUNT          456 a         1339 c         1138 g          443 t
ORIGIN      
        1 tcgcaggccg tgaccgaccc gggcgtggcc accgagccgt ggccgccgaa cagcgtcacg
       61 ccaccgccgt acccgaagct gacctcgtcg accccaccgc cgaaccggtc ggcgggcagc
      121 ccccgcccgg gcatctcgac gttgccgtcg atgacgaccg ggtcgccgtc tgcgggccac
      181 accgagatga acgcgccccc ggccagcgag acgatgcccg gctcggtgct catggccgcc
      241 atggccgccg caaccgcgac gtcgacggcg ttgcccccgg ccagagccac ctcgcgcccc
      301 gcggccagcg acaccggccc ggtcgcggcg atcgccactc gtccacccat gcgcggcatc
      361 ctctcaccgg cggccggatg ccgtctgctc acgtccgcga ccgcgccccc tccgtgtccc
      421 cgctgggctc acggccgggg gtagcgtgcg cgcatgacgc gatcgcccct ggccatcatc
      481 gccggcaccg gcttctacga cctggacgcc ctcgagaacc ccacgcagga gaccgtcgac
      541 accgtctggg gtcaggcgcg ggtcaccgag ggtgagtggc acggcatccc cgtcgtcttc
      601 ctgacgcgcc acggcgccgg ccactcggtg ccgccgcacc tggtcaacta ccgcgcgaac
      661 atccgggcgc tggccgacct cggggtgcgc gacgtcgtcg ccgtcaacgt cacggggtcg
      721 atcgacccga ccctgcaacc gggcgacctg ctctgcctcg acgacttcat cgacttcacc
      781 aagcagcggc cggtgacctt cttcgacggc tccacccccg agggcgtcgt gcacaccgac
      841 gtcatgacgc cctaccaccc cgccatccgc cgcgagatcc tcgacgccgc agccaccgcc
      901 gggcacacga tccgcgacgg tggggtctac gcgtgcttcg aggggccgcg cttcgagacc
      961 cgtgcggaga tccgcctggc cgcgctcgcc ggggccgacg tcgccggcat gacgggtgtg
     1021 cccgaggtga ccctcgccgt ggaggccggg cttcggtatg ccgcgatcag cctcgtcgtg
     1081 aaccccgcgt cgggcgtcgg cgacgcggac gagccgatcg ccatggacga gatcaacgcc
     1141 gtgctggccg cgtcgtcggc cacggtgctg gagatcctcg acgacctcgt ccacacccgc
     1201 gccaccttcc ccgaggagcc cgacgcatga gccgtctcat catcgagaac gccagctacg
     1261 tcgtcaccgt cgacgccacc gacaccgtcc tgcgcgacac caccatcacc atcgacgacg
     1321 gcgtcatcac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcggcaagc
     1441 tcgtgatgcc gggtctggtc aacctgcaca cccacctgcc gatgacgctg ctgcgtggtc
     1501 tcgccgagga cgtcgacctc gacggcttcc tggcccgggt gtgggcggcc gagggcgctg
     1561 tcatggacac cgacaccgtc gagctcggcg ccaccctggg cgccctggag tcactgcgcg
     1621 gcgggtgcac gacccagctc gacatgtact tccaccacga ggcgacccac cgcggggccg
     1681 tggccgcggg cagtcgtcac gtcatcgggc cggtgttctt cgacgggccg gggccggacg
     1741 gcctcacgtg ggagcagcgt ctggacaacc tgcgctcctg gcctgccgtg ctcgacgaga
     1801 tcggtggccc ctcggtgccg gtcggggcga tgccgcacgc cacctacacg tgctcgccgg
     1861 agggtttcgc gcaggtcgtc accgtgctgc gcgagctcgt cgccaacagt gagcgcgacg
     1921 gcatcctcac gacgcacgtg agcgagaccg ccgccgagaa cgccggcatc cgcgagcgct
     1981 acgacgcgac cccgaccgag ctgctggcgc gcgccgggtg ggtcgagccc gacctgccgc
     2041 tcgtcatcgg acacggcgtg cacctcagcg agggcgaccg aacgaccctg gcggccggag
     2101 gtgccgcggt cggtcactgc cccgggtcga acctcaagct cgccagcggc gccctgccgt
     2161 ggcaggtctt gcaggacagc ggcctgcgcg tcgggttggg caccgacggc tgctcgagct
     2221 cgaacgacct cgacatgtgg caggcgatgc gccaggccgc cctgctcgcg cggctgacca
     2281 gcgagcgccc tgacgtcgcg agcgcccacg aggtgctgcg cgcggccacc atcgagggcg
     2341 cccgcgcgct cgggatgggt gacgtgatcg gatcggtcga ggtgggcaag cgggccgacc
     2401 tcgtgctcgt cgacctcgag gcaccgcacc tcaccccggt gcacgacgtc gccgcgctgt
     2461 tggtgttcgc cgccgggcga ggagacgtca ccgacgtgct cgtcgacggg atgccggtcg
     2521 tgcgcgaccg tcggagcacc cgcctggaca cggggcagct tctcgaacgg gcccgggtcc
     2581 gcggggaggt cgcccgggca gcagccgcgg aggcggctgc gggccgatga ccagcgagag
     2641 cgccgacgag gtcatcgccc gcctcggcat gctgcccatc cccgaggagg gtgcctggtt
     2701 cgcgcccggc ccccgcaccc atggcctgaa cggcatcatc gccctgctca ccgaccgccc
     2761 cgacgggttc tcggccatgc accgcctcac catcgacgag ggctggcagt ggatggccgg
     2821 tgcccccgcc accctcgtgc gcctgcaccc cgacggcacc ggcgactcca cgaccctcgg
     2881 cccggactcc ccccaggccc tggtcaccaa gggctcgtgg atgggagccc gcacccacgg
     2941 cgagtggacc ctgttctcct gctggtgcgc ccctgcgttc gagcccgagc acttcgagct
     3001 cggcgagcgc tcggccctgc tcgacgcctt ccccgaccac gccgccgaga tcaccgcgct
     3061 gacccgcacc caccccatcg gtcagatgcg atgacggccc tggtcaccgg cgccagcggc
     3121 gggctcgggc gcggcatcgc cgtcgcgctg gctgccgccg gtcacgacat cggcgtgcac
     3181 ttccggtcgg atgccgtcgg tgcggccgcc gctgtcaccg acgtcgaggc ggtgggtcgc
     3241 cgcgcgacgg ccctgcacgc cgacctcgcc gtcgacgacc ctgccgagct cgaccgggcc
     3301 tgtgacgacc tcctcgacgc ctgcaccgca gcgctcggcg cccccgaggt ggtggtgctg
     3361 aacgccttcc cccagcacgt cgtggcctgg ggtgacctcg acacggcggc ctgggacacc
     3421 taccaccgcg gtgggctgcg ccccacgacc agcctgctcc accgggccgc cgcccg
//
LOCUS       DAOSOC010000221         3461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_27308_length_3461_cov_8.162948, whole genome shotgun sequence.
ACCESSION   DAOSOC010000221 DAOSOC010000000
VERSION     DAOSOC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27308_length_3461_cov_8.162948"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1495)
                     /locus_tag="PL014_12095"
     CDS             complement(14..1495)
                     /locus_tag="PL014_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOT57877.1"
                     /translation="MGAEVSSQSYSREERQRYREKVRQNLDVFEKMLNTSSFEFDRPM
                     TGLEIELNLVDADMQPHFHNAEVLAAIADEDYQTELAQYNIELNVPPRPLPGDSALEL
                     ETELRASLNRAAAKAQEVGSKIVAIGILPTIMPEHYEGEWISRNNRYTALNDSIFVAR
                     GEDIYLDIEGPSGERVATYCDSIAPESACTSVQLHLQVTPSEFAAHWNAAQALCAPQV
                     ALAANSPFFFGKRLHAETRIELFKQATDTRPIELKNQGVRPRVFFGERWITSIFDLFE
                     ENVRYFPTLLAETTDEDPMAKLEAGEAPELAELRLHNGTVYRWNRPIYDIPGGVPHLR
                     VENRVLPAGPTIIDVLANAAFYYGAIRMLAHEDRPVWTKMSFAACEENFNRAARDGID
                     AHLYWPGFGELSADELMLRHLLPLAHEGLRQWGVSDAVRERFLGVIEGRCTTGVNGAV
                     WSTKAVEAFESGGCTRSEALSRMLELYAVHMDSNEPVHTWPLP"
     gene            1589..2779
                     /locus_tag="PL014_12100"
     CDS             1589..2779
                     /locus_tag="PL014_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOT57878.1"
                     /translation="MRLIHTSDWHLGRSFHQVGLLGAQASYLDHLVDVVRREKVDAVL
                     VSGDVYDRAMPAPDTVALLSQALQRLVDAGAQVIISSGNHDSAARLGFGAGLLERTGV
                     HLRTSLADVARPVLLAGAAVYPLPYLEPSLAADALGATERTHAGVLRAAMDAVRADAA
                     ARPGVPVVAMAHAFVSGGVGSESERDISVGGVSAVPPTVFAGAHYAALGHLHGPQQVA
                     PGVRYSGSPVAMSFSEVNHRKVSLLVDLDGRSVSVESVEAPVERPLAVLRGTLEEVLA
                     DPRHRAAESAWCQVTLTDSVRPLGAMDQVRRRFPHTLELRFDPQGLAVPLRPYAARAA
                     SRSDLDVCCDFLEHVRGGRAASEQERELLAQAVEESRLARRARDDEGVAGPAADAART
                     VGAA"
     gene            2776..>3461
                     /locus_tag="PL014_12105"
     CDS             2776..>3461
                     /locus_tag="PL014_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOT57879.1"
                     /translation="MRVHRLEIEAMGPFADPVSIDVDALSADGLFLIHGPTGSGKTSL
                     LDAICFALYADVPGTRSKRGLRSDHAAADAVPRVTLELTAGGRRLRITRSPEFARPKR
                     RGTGTVSVQAMVTLEEHLDGRWQSLSTRNDEVADIVKDVLGMGMTQFSKVVLLPQGEF
                     AAFLRSSPEDRREVLERLFDISAFSDVEAWLAQARRDAREELDTARSTLATGLARVED
                     VLAELGEPLTS"
BASE COUNT          466 a         1224 c         1223 g          548 t
ORIGIN      
        1 ggcatccggt cgttcagggc agcggccagg tgtgcaccgg ctcgttggag tccatgtgca
       61 cggcgtacag ctcgagcatc cggctcagcg cctcgctgcg ggtgcagcca cctgactcga
      121 aggcctccac cgccttggtg gaccacaccg ctccgttgac gccggtggtg cagcgcccct
      181 cgatgacacc gaggaaccgt tcgcgaacgg catccgacac cccccactgg cgaaggccct
      241 cgtgcgccag cggcagcagg tgacgcagca tcagctcgtc ggccgagagc tcaccgaacc
      301 cgggccagta caggtgcgcg tcgatgccgt cacgggcggc ccggttgaag ttctcctcgc
      361 acgccgcgaa gctcatcttg gtccagaccg gacggtcctc gtgggccagc atccggatcg
      421 cgccgtagta gaacgcggcg ttggccagca cgtcgatgat cgtcggcccg gccggcagca
      481 cccggttctc gacccgcagg tgcggcacac caccggggat gtcgtagatc ggccggttcc
      541 agcggtagac ggtgccgttg tgcaggcgca gctcggcgag ctcgggcgcc tcgccggcct
      601 cgagcttggc catcgggtcc tcgtccgtgg tctccgcgag cagcgtcggg aagtagcgga
      661 cgttctcctc gaacaggtcg aagatcgagg tgatccaccg ctcgccgaag aacacccgcg
      721 ggcgcacccc ttggttcttc agctcgatcg ggcgggtgtc ggtggcctgc ttgaacagct
      781 cgatgcgggt ctcggcgtgc aggcgcttgc cgaagaagaa gggcgagttg gccgcgaggg
      841 ccacctgcgg ggcgcacagg gcctgcgccg cgttccagtg ggccgcgaac tccgacggcg
      901 tgacctggag gtggagctgg accgacgtgc aggccgactc gggggcgatg gagtcacagt
      961 acgtggcgac ccgctcgccg gaggggccct cgatgtcgag gtagatgtcc tcgccgcggg
     1021 cgacgaagat cgagtcgttg agggcggtgt agcggttgtt gcggctgatc cactcgccct
     1081 cgtagtgctc gggcatgatc gtcggcagga tgccgatggc gacgatcttc gagccgacct
     1141 cctgggcctt ggccgcagcc cggttcaggc tcgcgcgcag ctcggtctcg agctcgagcg
     1201 cggagtcacc gggcaggggc cgcggcggga cgttgagctc gatgttgtac tgcgcgagct
     1261 cggtctggta gtcctcgtcc gcgatggccg cgagcacctc ggcattgtgg aagtgcggct
     1321 gcatgtcggc gtcgacgagg ttgagctcga tctcgaggcc ggtcatcggc ctgtcgaact
     1381 cgaaggagct cgtgttgagc atcttctcga acacgtcaag gttctggcgc accttctcgc
     1441 ggtagcgctg ccgctcctcg cggctgtaac tctgcgacga aacctctgct cccatggcgt
     1501 cacccaacca cgtcctgagc tcatgcggta cccggaaccc gcgtttcggt cacacggcct
     1561 gtcagaccca cctcctagcg tgtgagacat gcggctgatc cacacctccg actggcacct
     1621 ggggcgttcg ttccaccagg tggggctcct cggcgcccag gcctcctacc tcgaccacct
     1681 ggtcgacgtg gtgcgtcgcg agaaggtcga cgcggtgctc gtcagcggcg acgtctacga
     1741 ccgggcgatg cccgcgcccg acaccgtggc cctgctgtcg caggcgctcc agcggctggt
     1801 cgacgccggc gcccaggtga tcatcagcag cggcaaccac gactcggcgg cccgcctggg
     1861 cttcggcgcc gggctgttgg agcgcaccgg cgtgcacctg cgcacctcgc tcgccgacgt
     1921 cgcccgcccg gtcctgctcg ccggggccgc ggtctacccc ctgccctacc tcgagccctc
     1981 gctggccgcc gacgccctcg gggcgacgga gcgcacccac gccggggtgt tgcgggcagc
     2041 catggatgcc gtgcgggcgg atgccgctgc gcgtccgggg gtgccggtgg tcgccatggc
     2101 gcacgccttc gtctccggcg gggtgggcag cgagtccgag cgggacatct cggtcggtgg
     2161 cgtctccgca gtgccgccca cggtgttcgc cggggcgcac tacgccgccc tggggcacct
     2221 gcacgggccc cagcaggtcg cgcccggggt gcgctacagc ggttcacccg tggcgatgtc
     2281 gttctccgag gtcaaccacc gcaaggtcag cctgctcgtc gacctcgatg gccggtcggt
     2341 gtcggtcgag tccgtcgagg caccggtcga gcggccgctc gccgtgctgc gcggaacgct
     2401 cgaggaggtg ctcgccgacc cccgccaccg cgcggccgag agtgcgtggt gccaggtgac
     2461 cctcaccgac tccgtccggc cgctgggggc gatggaccag gtgcgccgcc gcttccccca
     2521 caccctcgag ctgcgcttcg acccccaagg gctcgccgtg ccgctgcggc cctatgccgc
     2581 ccgcgccgcg agccgctccg acctcgacgt gtgctgcgac ttcctcgagc acgtgcgcgg
     2641 cggtcgtgcg gccagtgagc aggagcgtga actgctcgcc caggcggtcg aggagtcgcg
     2701 actggcgcgg cgggcccgtg acgacgaagg ggtggccggc cccgctgccg acgcggcgcg
     2761 cacggtgggt gcggcatgag ggtgcaccgg ctcgagatcg aggcgatggg gccattcgcc
     2821 gaccccgtgt ccatcgacgt cgacgcgttg tccgcggacg ggctcttcct catccacggt
     2881 ccgaccgggt cgggcaagac cagcctgctc gacgccatct gcttcgcgtt gtacgccgac
     2941 gtaccgggca ctcgctcaaa gcgcgggctg cgctcggacc acgccgctgc ggatgccgtc
     3001 ccgcgcgtca cgctcgagct caccgcgggt ggtcggcggc ttcgcatcac gcgctccccc
     3061 gagtttgccc ggcccaagag gcgcggtacg ggcacggtgt cggtccaggc catggtgacc
     3121 ctcgaggagc acctcgacgg ccggtggcag tccctcagca cccgcaacga cgaagtggcc
     3181 gacatcgtca aggacgtcct cggcatgggg atgacgcagt tctccaaggt ggtgctgctg
     3241 ccccagggcg agttcgccgc cttcctgcgt tcctcccccg aggaccgtcg ggaggtcctg
     3301 gagcgactgt tcgacatcag cgccttcagc gacgtggagg cttggctcgc ccaggcccgt
     3361 cgggacgcca gggaagagct cgacaccgcc cgcagcacct tggccaccgg gctggcccgg
     3421 gtcgaggacg tcctggcgga gctcggcgaa ccgctgacct c
//
LOCUS       DAOSOC010000222         3430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_27644_length_3430_cov_8.947556, whole genome shotgun sequence.
ACCESSION   DAOSOC010000222 DAOSOC010000000
VERSION     DAOSOC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27644_length_3430_cov_8.947556"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..2237
                     /locus_tag="PL014_12110"
     CDS             321..2237
                     /locus_tag="PL014_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HOT57880.1"
                     /translation="MAKSAAATPSGSGGSGGPGSPSPAQVAQQARARTPIEVTGAQSL
                     VLSLEAMDVDTVFGIPGGAILPAYDPLLDSVKVRHILVRHEQGAGHAAEGYAAATGRV
                     GVCMATSGPGATNLVTPLADAHMDSVPIVAITGQVNSAAIGTDAFQEADIRGITMPIT
                     KHNYLVTNADEIPRIIAEAFHIASTGRPGPVLVDISKDALQARTTFSWPPRIDLPGYR
                     PVTRPHGKQIREAARLIAAAERPILYVGGGVIRAEASEELRRFVELSKIPVVTTLMAR
                     GALPDSHPLHLGMPGMHGSVSAVTGLQKSDLIIALGARFDDRVTGQLSTFAPFAKVIH
                     ADIDPAEIGKNRRADVPIVGDCKAVITDLTEAVDSDIDAGRGGDYSEWRERVAGWKES
                     FPVGYTEPEEASLAPQYVIERLSAIVGPEAVYVAGVGQHQMWAAQFVQYERPNAWLNS
                     GGLGTMGYAVPAAMGAQVGEPDRTVWAIDGDGCFQMTNQELATCVINNIPIKVAVINN
                     SSLGMVRQWQTLFYNERYSNTDLHTSVGSRVPDFVKLADAYGCAGFRCERPEDVDDTI
                     RAAMAVNDRPVIVDFVVHRDAMVWPMVAAGVSNDLIQVARDTAPQWDREESEALEQDL
                     DADHDGVPSGSPRN"
     gene            2252..2764
                     /gene="ilvN"
                     /locus_tag="PL014_12115"
     CDS             2252..2764
                     /gene="ilvN"
                     /locus_tag="PL014_12115"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOT57881.1"
                     /translation="MSKHTLSVLVENKPGVLARIAALFSRRGFNIDSLAVGETEYPDV
                     SRMTVVVDVEDLPLEQVTKQLNKLVEVLKVVELEPTSAVQRQVMLIKVRADAATRSQV
                     LEMVQMFRAKVVDVATESLTIEATGTTDKLRALLDVLEPYGVKELVQSGIVAIGRGPR
                     SITDRSLRTA"
     gene            2855..>3430
                     /gene="ilvC"
                     /locus_tag="PL014_12120"
     CDS             2855..>3430
                     /gene="ilvC"
                     /locus_tag="PL014_12120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOT57882.1"
                     /translation="MAEMFYDDDADLAIIQGRKVAVIGYGSQGHAHALNLRDSGVDVR
                     VGLAEGSKSRAKAEAEGLRVLSVAEAVAEADLVVILTPDQVQRTVYANEIQPNLAEGA
                     ALLFGHGFNIRFGYITPEAGSDVLMVAPKGPGHLVRREFVDGRGVPVLLAVEQDASGH
                     AWDLAKSYAKAIGGLRAGGIKTTFTEECETDL"
BASE COUNT          546 a         1301 c         1107 g          476 t
ORIGIN      
        1 ctagcggagg gcgcggctgc ggcgccccgg catccacgac gctcacccac ggcgggtgag
       61 gcacgtcacc ttcccggatc gtgggatcgg ccgtctcgcc ggttgacacg accaccccgg
      121 cgggggacag ttcaggggtg gcccgagtcc tcgtactttc acggcgcgtc tgaccgatca
      181 ggcagacgcg ctccctccgt cgtcccccgt gggactggag ggtttttttg tgcacggacg
      241 acggccgcga gcaagcggca aaccccagag agcggtgaac ggcagtgagc gaaacgaggc
      301 cggcgaaccc ggttgtcccg atggccaaga gcgcggccgc gaccccgagc ggttccgggg
      361 gctccggtgg ccccggcagc ccgagcccgg cgcaggtcgc gcagcaggcc cgcgcccgga
      421 ccccgatcga ggtgaccggc gcgcagagcc tcgtgctctc cctcgaggcg atggacgtcg
      481 acaccgtctt cggcatcccc ggtggggcga tcctgccggc gtacgacccg ctcctggact
      541 cggtcaaggt gcggcacatc ctcgttcggc acgagcaggg cgccggccac gccgccgagg
      601 gctatgccgc cgccaccggg cgggtcgggg tctgcatggc cacgtccggc cccggggcca
      661 ccaacctcgt gaccccgctc gccgatgcgc acatggactc cgtgccgatc gtggcgatca
      721 ccggccaggt caactcggcc gccatcggca ccgacgcctt ccaggaggcc gacatccgcg
      781 gcatcacgat gccgatcacc aagcacaact acctggtgac caatgccgac gagatcccgc
      841 ggatcatcgc cgaggccttc cacatcgcct ccaccggccg ccccggcccg gtgctcgtcg
      901 acatctccaa ggacgcgctc caggcccgca ccaccttctc gtggcctccg cgcatcgacc
      961 tgcccggcta ccgcccggtg acccgccccc acggcaagca gatccgtgag gccgcccgcc
     1021 tcatcgccgc ggccgagcgt cccatcctct acgtgggcgg cggcgtcatc cgcgccgagg
     1081 ccagcgagga actgcgccgc ttcgtcgagc tgagcaagat ccccgtcgtg accaccctga
     1141 tggcgcgtgg ggccctgccc gacagccacc cgctccacct cgggatgccg gggatgcacg
     1201 ggtcggtctc ggccgtcacc ggcctccaga agtccgacct catcatcgcc ctcggcgccc
     1261 ggttcgacga ccgcgtcacc ggccagctgt cgaccttcgc gccgttcgcg aaggtcatcc
     1321 acgccgacat cgaccctgcc gagatcggca agaaccggcg ggccgacgtc ccgatcgtcg
     1381 gcgactgcaa ggcggtgatc accgacctca ccgaggccgt cgactccgac atcgacgcgg
     1441 gccgtggcgg cgactacagc gagtggcgcg agcgggtcgc aggctggaag gagtccttcc
     1501 ccgtcggcta caccgagccc gaggaggctt ctctggcacc gcagtacgtc atcgagcggc
     1561 tcagcgccat cgtcggcccg gaggccgtgt acgtcgccgg cgtcggccag caccagatgt
     1621 gggcggccca gttcgtgcag tacgaacgcc ccaacgcctg gctgaactcc ggtggcctcg
     1681 ggaccatggg ctacgccgtc ccggccgcga tgggcgccca ggtcggtgag cccgaccgca
     1741 ccgtgtgggc catcgacggc gacggctgct tccagatgac gaaccaggag ctcgcgacct
     1801 gcgtcatcaa caacatcccg atcaaggtcg ccgtcatcaa caacagctcg ctcggcatgg
     1861 tgcgccagtg gcagaccctg ttctacaacg agcgttactc caacaccgac ctgcacacct
     1921 cggtgggctc gcgggtgcct gacttcgtca agctcgccga cgcctacggc tgcgccgggt
     1981 tccggtgcga gcgccccgag gacgtcgacg acaccatccg tgccgccatg gccgtcaacg
     2041 accgcccggt catcgtcgac ttcgtcgtcc accgcgacgc gatggtctgg ccgatggtcg
     2101 cagccggggt gagcaacgac ctcatccagg tcgcccgcga caccgcgccg cagtgggacc
     2161 gcgaggagtc cgaggcgctc gagcaggacc tcgatgccga tcacgacgga gtcccctcgg
     2221 gctccccccg gaactgaaag cggtgactgc catgtcgaag cacaccctgt ccgtcctcgt
     2281 cgagaacaag cccggcgtgc tcgcccgcat cgccgcgctg ttctcccgtc ggggcttcaa
     2341 catcgactcc ctcgccgtcg gtgagaccga gtaccccgac gtctcgcgga tgaccgtcgt
     2401 cgtcgacgtc gaggacctcc cgctcgagca ggtgaccaag cagctcaaca agctcgtcga
     2461 ggtgctcaag gtcgtcgagc tcgagccgac gtcggccgtc cagcgccagg tcatgctcat
     2521 caaggtgcgt gccgacgccg ccacccgcag ccaggtgctc gagatggtgc agatgttccg
     2581 ggccaaggtc gtcgacgtcg ccaccgagtc gctcaccatc gaggccacgg gcaccaccga
     2641 caagctgcgt gccctgctcg acgtcctcga gccctacggc gtcaaggagc tcgtgcagtc
     2701 cgggatcgtc gcgatcggtc gcggcccgcg atccatcacc gaccggagcc tgcgcaccgc
     2761 ctgagaccac gccgcacccc acccggggca cggcatacag ttccagtcga acttccaccc
     2821 acagtcacca ccaccacgag gagaggccga gacgatggcc gagatgttct acgacgacga
     2881 cgccgacctg gcgatcatcc agggccgcaa ggtcgcggtc atcgggtacg gcagccaggg
     2941 ccacgcccac gcgctgaacc tgcgcgactc cggggtcgac gtgcgcgtcg ggctcgccga
     3001 gggctcgaag tcccgcgcca aggccgaggc cgagggcctg cgggtgctca gcgtggccga
     3061 ggccgtcgcc gaggccgacc tcgtcgtcat cctgacgccc gaccaggtgc agcgcaccgt
     3121 ctacgcgaac gagatccagc ccaacctcgc cgagggtgcc gcgctgctgt tcggccacgg
     3181 cttcaacatc cgcttcggct acatcacccc cgaggctggt tccgacgtgc tcatggtcgc
     3241 ccccaagggc cccggccacc tcgtgcgccg cgagttcgtc gacggccgcg gtgtgcccgt
     3301 gctgctcgcg gtcgagcagg acgcctccgg ccacgcctgg gacctcgcga agtcctacgc
     3361 caaggccatc ggtggtctgc gcgccggcgg catcaagacg accttcaccg aggagtgcga
     3421 gaccgacctg
//
LOCUS       DAOSOC010000223         3378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_28188_length_3378_cov_8.465543, whole genome shotgun sequence.
ACCESSION   DAOSOC010000223 DAOSOC010000000
VERSION     DAOSOC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28188_length_3378_cov_8.465543"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..1236)
                     /locus_tag="PL014_12125"
     CDS             complement(307..1236)
                     /locus_tag="PL014_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOT57883.1"
                     /translation="MGADSGGTTIRQTLRRLGGVTTWKELRSSHPKRRILRAVRDGEL
                     VRASRGHYVLPTASAHVTAAQSCAGVLSHLSAAIHHGWKVKSPPGHAWVTVPRKRHVR
                     KKQRHGIRPHHADLTAKDVEHGVTTPLRTVIDCARVLPFDEALVVADSALRARAVTRG
                     ELADAVRHLRGPGAVAARRVVKHADGRAANPFESVLRALCIEEGFELTPQVTIAETGL
                     YAVVDLGSEALRLAVEADGFETHGTRGGLRKDCERHTLFAIYAYSSLRFAYEHVMFEP
                     DWVRWALRTWRAVRAGQALTDPPLRSHRQAKGS"
     gene            1516..2334
                     /locus_tag="PL014_12130"
     CDS             1516..2334
                     /locus_tag="PL014_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT57884.1"
                     /translation="MSASPPPSVTAPWAGGPVTPRAHCVLAPNPGPMTLDGTNTWVLL
                     EPGATEAVVVDPGPLDEAHLAAVLAVVADAGARVALTVLTHGHADHAEAVPRFAELTG
                     APVRAVGRGHDDLADGDVVRTGGLELRIVATPGHTSDSISLALPADHALLTGDTVLGR
                     GTTVVAHPDGELDAYLDSLDRLHALTGDGSVTAILPGHGPLVPDAGVMVAYYRTHRAE
                     RLDQVRQALADGAASEIDVVEGVLQRVYADVPSAVWPAARMSIRAQLEYLDRER"
     gene            complement(2407..3084)
                     /locus_tag="PL014_12135"
     CDS             complement(2407..3084)
                     /locus_tag="PL014_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT57885.1"
                     /translation="MDHDVVRRAPLFAALDEAAAESLIANMSPALLERGDVLFHEGDQ
                     GDRLYVIGEGKIKLGRSSSDGRENLLAILGPGEMFGELSLFDPGPRTATATAVAETQV
                     VSMGHDQLKDFLRTHPGVAPTLLSALARRLRRTNDVLADLVFTDVPGRVAKALLDLAD
                     RFGRPVDDGLLVAHDLTQEELAQLVGASRETVNKALADFASRGWLRLEARAVLLTDVE
                     RLKRRAR"
BASE COUNT          448 a         1227 c         1230 g          473 t
ORIGIN      
        1 aggtgcggat gctgccgccc accgtgatct cgctcgagga gctcgcggac ttcgcggatg
       61 ccgcggcgtt cctcgcgcac cgacctcgcg tcgcgcgggt ggtgcccgaa ctcgtggcgg
      121 gggcggacgg catccccatg ttgcgcaccg tcctgcccta gccgcaccgg cgtacctcct
      181 gccccaaacc caacctccgc ccctcctgtc ctagcccggg tctgcacccg gccgggcggc
      241 cctgacgaca ggcaaagggg cctgggaccc cgtcgagccc cagccatgcc gcgaccatgc
      301 gtcccttcac gacccctttg cctgtcggtg ggatcgcagc ggcgggtcgg tcagcgcttg
      361 gccggcccgc accgctcgcc aggtgcggag cgcccagcgc acccagtcgg gctcgaacat
      421 cacgtgctcg taggcgaacc gcagcgacga gtacgcgtag atggcgaaca gggtgtggcg
      481 ctcgcagtcc ttgcgcaggc cgccccgggt gccgtgggtc tcgaagccgt cggcctcgac
      541 ggccagccgc agggcctcgc tgccgagatc gaccacggcg tagagccccg tctcggcgat
      601 cgtcacctgc ggggtgagct cgaacccctc ctctatgcac agcgcgcgca gcaccgactc
      661 gaacgggttg gccgcgcgcc cgtcggcatg cttgacgacc cgccgtgccg caaccgcacc
      721 cggtccgcga aggtgccgca cggcatccgc cagttccccg cgcgtgacgg ctcgggcgcg
      781 aagggcggag tcggccacca cgagcgcctc gtcgaagggc agcacccggg cgcagtcgat
      841 caccgtgcga aggggcgtgg tgaccccgtg ctccacgtcc tttgccgtca ggtccgcatg
      901 gtggggccgg atgccgtggc gctgcttctt gcgcacgtgt cgtttgcgcg gcaccgtgac
      961 ccacgcgtgg ccaggtggtg acttgacctt ccacccatgg tgaatggccg cgctcaggtg
     1021 cgacaacaca ccagcgcagg actgtgcggc ggtcacatgg gccgacgccg tgggcaggac
     1081 gtagtggccg cgggacgccc tcacgagctc cccatccctc acggctcgca ggatgcggcg
     1141 cttggggtgg gacgagcgca gctccttcca cgtcgtgacc ccgccgaggc gccgcagggt
     1201 ctgccggatg gttgtccctc cgctgtctgc ccccatcggc ccatcgtgcc gccaggaggg
     1261 gcgctcagcg ggttgtccac aggcctgatc gcgggctgac gcgaagcggg gtgggcctgc
     1321 cgacaggcaa agggccggcg cagaagcgct ctgacccggg attcctgtgc tcaggcgcga
     1381 gcggcaggtc gctttgcctg tgagtgggcc cgccctcgag tcgcgccgtc gcagggtggt
     1441 caggaccggg tgcgggggca gcgcaggggg tacggcagcg cagggtgtgg ggccggcggg
     1501 gcatactgcg gtggcatgag cgcgtccccg ccgccgtcgg tcaccgcacc ctgggcaggc
     1561 ggccccgtga cgcctcgcgc ccactgcgtg ctcgccccca accccggccc gatgaccctg
     1621 gacggcacga acacgtgggt gctcctcgag cccggagcca ccgaggccgt ggtcgtcgac
     1681 cccgggccac tcgacgaggc gcacctcgcc gccgtgctcg cggtggtcgc ggatgccggg
     1741 gcgcgcgtcg cgctcaccgt cctcacccac gggcacgccg atcacgccga ggccgtgccg
     1801 cggttcgccg agctcaccgg ggcccccgtg cgcgcggtcg gtcgtggcca cgacgacctc
     1861 gccgacggcg acgtcgtgcg caccggaggc cttgaactga ggatcgtggc aaccccgggg
     1921 cacacctcgg actcgatctc gctcgcccta cccgccgacc acgccctgct caccggcgac
     1981 accgtgctcg gtcgaggcac caccgtggtg gcccaccccg acggtgagct cgacgcctac
     2041 ctcgactccc tcgaccggct ccacgccctc accggtgacg gatcggtgac cgcgatcctg
     2101 cccggacacg gcccgctcgt cccggatgcc ggggtgatgg tcgcgtacta ccgcacccac
     2161 cgtgccgagc ggctggacca ggtgcgccaa gcgctcgccg acggcgcggc atccgagatc
     2221 gacgtcgtcg agggcgtgct gcagcgggtc tacgccgacg tgccgagcgc ggtgtggcca
     2281 gctgcgcgga tgtcgattag ggcgcagctg gagtacctcg accgggagcg gtgaggggcg
     2341 gccgtcagcg gtctgcggtg ccggcggtgc cgccggtgcc agcgccagcg ggccgcgacg
     2401 cagggatcag cgggcgcggc gcttcagtcg ctcgacgtcg gtcagcagca cggcgcgcgc
     2461 ctcgaggcgc aaccagcccc gcgacgcgaa gtcggccagt gccttgttca ccgtctcgcg
     2521 ggaggcgccg acgagctgcg cgagctcttc ctgggtgagg tcgtgcgcga cgagcaggcc
     2581 atcgtcgacc gggcggccga agcggtcggc caggtcgagc agcgccttgg cgacgcggcc
     2641 gggcacgtcg gtgaacacga ggtcggcgag cacgtcgttg gtgcggcgca ggcggcgggc
     2701 gagggcggag agcagggtcg gggccacccc ggggtgcgtg cgcaggaagt ccttgagctg
     2761 gtcgtggccc atcgacacga cctgcgtctc ggcgaccgcg gtggccgtgg cggtgcgcgg
     2821 cccgggatcg aacagcgaca gctcgccgaa catctcgccg gggccgagga tggccagcag
     2881 gttctcgcgg ccgtcgctgc tggagcgccc gagcttgatc ttgccctcac cgatgacgta
     2941 gagccggtcg ccctggtcgc cttcgtggaa gagcacgtcg ccacgttcca gcagcgctgg
     3001 gctcatgttg gcgatcagtg actcagccgc cgcctcatca agggcggcga acagcggggc
     3061 tctgcgcacg acgtcgtgat ccacgggcca cagtgtgcca gagtcacccc gaggccctcg
     3121 ccctcatcaa caacgtgggt gaaaatcgtc gtccaggcga cgatcttcac ccacgttcgc
     3181 gggaagggtc gggggagagg gggctcggat gccgttcgcg cacctctgcg ctgacagggt
     3241 cacgtcgtag gctgcgcggg tgtccgtgac cccctccgcc gccgtgcggc gcctgcgcgt
     3301 cggtgaggag tcgccggtgg cgctgacccg gcgggcgcgg cgcacctacc gggcgctgct
     3361 cgagcgctac ccgtatgc
//
LOCUS       DAOSOC010000224         3377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_28205_length_3377_cov_9.288983, whole genome shotgun sequence.
ACCESSION   DAOSOC010000224 DAOSOC010000000
VERSION     DAOSOC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28205_length_3377_cov_9.288983"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PL014_12140"
     CDS             complement(<1..354)
                     /locus_tag="PL014_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57886.1"
                     /translation="MTPDDLLSRFHTQIRLADKDAAPGFVVEHDGPVHRTYPADAGAP
                     GAMVECPQGLGEDPGHWIARQVEFFRGRGQEVEWKTYGYDEPADLGERLVAAGFVAQA
                     PEVVLLGRCTDLVHDV"
     gene            519..1886
                     /locus_tag="PL014_12145"
     CDS             519..1886
                     /locus_tag="PL014_12145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT57887.1"
                     /translation="MDLSTVRDLAAATPATRNRVVDLLRAAAIVVVVLGHWLMAAVVV
                     RDGELEIGHLLELAPWTHPLTWVFQVMPVFFLVGGYANALSWRSARAKNLPYAGWLRA
                     RLRRLLTPVVPLLLVWLLAVVVAHAAGVPTATLRTASQVALVPTWFLAAYVVVCSVAP
                     ATLWVWERWGWSSIVGGLALGCLIDLVSLGTDTLTVGFANYLVVWATVHQVGYAWLDG
                     RLAGTGRRLTLAAVGLVGVVGLVWAGPYPVSMIGLDGAAVNNSYPTRVTLGFLGLLQA
                     GLVLSLEPVLARWLQRPRAWSATVLLNTRIMTLYLWHLTAMVLVIGLSLLLGGPGLGV
                     TPMTGAWWATRPLWWAVLGLVTLGFITVLGRFENPRRDDSPAPPVWLPVLVTVLACGG
                     LGFMAAKGIVGADGVHWWWPVLTVGAVALLRLPVTSRRRATAGSPHGRETGGQASPRR
                     NRRPG"
     gene            complement(1795..2844)
                     /locus_tag="PL014_12150"
     CDS             complement(1795..2844)
                     /locus_tag="PL014_12150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOT57888.1"
                     /translation="MRPAPALSVLDLVPVRADQSSGDAVAAMRSLAKVADENGYRRYW
                     LAEHHNMPSIASTNPPVLAAIVAAATERIRVGSGGVMLPNHAPLVVAEQFALLEAAFP
                     GRVDLGIGRAPGTDQVTAWALRSGAGGVEADAVDRFPDHVDHVLAMMSPAGAALRLGS
                     GEHVLRATPHATSVPMIWLLGSSDFSARLAASRGLPYVFAHHFSGRGTAEALALYREG
                     YQPSPEHPEPRTFLTVNVVVAPTQAQAQRLVQPMVRTMVALRTGQPLMPQESVEEAEA
                     RGVVAAHQPLADEMASRWVVGDPQQAAARVRELAATFDVDEVMVSPVAGSFAGTPVRR
                     SPAREETLRLLADAM"
     gene            complement(2885..>3377)
                     /locus_tag="PL014_12155"
     CDS             complement(2885..>3377)
                     /locus_tag="PL014_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOT57889.1"
                     /translation="PFHVAIENWGHDFNIGSVVRTANAFNAAAFHIVGKRRWNRRGAM
                     VTDRYQHEHHHPDVDDLVQWASGAGTQADGIPIIGIDNLPGSVPLETHDLPRECVLVF
                     GQEGPGLSEPMRAACTAVLHIEQFGSTRSINAGAAAAIAMHAWVRRHVFHQRPLPAGP
                     PSE"
BASE COUNT          410 a         1215 c         1233 g          519 t
ORIGIN      
        1 gacgtcgtgc acgagatcgg tgcaccggcc gagcagcacg acctcggggg cctgcgcgac
       61 gaacccggca gccacgagcc gctccccaag gtcggcgggc tcgtcgtagc cgtaggtctt
      121 ccactcgacc tcctgccccc tgcccctgaa gaactccacc tgccgggcga tccagtgccc
      181 cgggtcctcg cccagaccct gcgggcactc gaccatcgcg cccggcgcac cggcatccgc
      241 ggggtaggtg cggtggaccg gcccgtcgtg ctcgacgacg aaacccggtg ccgcgtcctt
      301 gtccgcgagg cggatctggg tgtggaatcg gctgaggagg tcgtcaggcg tcatggaccc
      361 attgcacgcc atcggatgcc gttcgcgcat ccgcgttttc ccctcgcctc ctcccttgcg
      421 tccggcatct tggctgcccc gacgaccacc ctgccggacg caacgctggg tcgccgtact
      481 cacgcgcggc ccccaccgaa cccgtcaggc tgtgaggcat ggacctgtcc accgtgcgtg
      541 acctcgccgc cgcgaccccc gccacccgca accgggtcgt cgacctgctg cgcgccgcgg
      601 ccatcgtcgt ggtcgtgctc ggccactggc tgatggccgc cgtcgtggtc cgcgacggtg
      661 agctcgagat cggtcacctc ctcgagctcg ccccgtggac ccacccgctc acctgggtgt
      721 tccaggtgat gccggtgttc ttcctcgtcg ggggctacgc caacgccttg tcatggcgct
      781 cggcgcgggc gaagaacctg ccgtatgccg ggtggttgcg ggcgcggctg cggcggctcc
      841 tcacgccggt ggtcccgctc ctgctggtct ggctgctcgc ggtggtcgtg gcccacgcgg
      901 caggggtgcc gaccgcgacg ctgcgcacgg catcccaggt ggccctggtg ccgacgtggt
      961 tcctcgccgc ctacgtcgtc gtctgctcgg tcgcccccgc cacgctctgg gtgtgggagc
     1021 gctggggctg gtccagcatc gtcggcgggt tggcactcgg gtgcctgatc gacctcgtga
     1081 gcctcggcac cgacacgctg accgtgggct tcgcgaacta cctcgtcgtg tgggcgacgg
     1141 tgcaccaggt cgggtacgcc tggctcgacg gtcgcctggc cggcaccggt cgacggctga
     1201 ccctggccgc cgtcgggctc gtcggtgtcg tggggctggt gtgggccggc ccctaccccg
     1261 tgtcgatgat cgggctggac ggggccgccg tgaacaactc ctacccgacg cgggtgacct
     1321 tggggttcct cggcctgctg caggccgggc tcgtgctgag cctcgaaccg gtgctggccc
     1381 gctggttgca gcggccgcgc gcctggtcgg cgacggtgct gctcaacacc cggatcatga
     1441 cgctctacct gtggcacctg acggccatgg tgctcgtcat cgggctctcg ctgctgctcg
     1501 gcggtccggg cctgggcgtc accccgatga ccggggcctg gtgggcgacc cggccgctgt
     1561 ggtgggccgt gctcggcctc gtgaccctcg ggttcatcac cgtgctcggc cggttcgaga
     1621 acccgcggcg cgacgactca cccgctccgc cggtgtggct gccggtgctc gtcaccgtgc
     1681 tcgcctgcgg ggggctcggc ttcatggccg ccaagggcat cgtcggcgcc gacggcgtgc
     1741 actggtggtg gcccgtgctc accgtcgggg cggtggccct gctgcgcctg ccggtcacat
     1801 cgcgtcggcg agcaaccgca gggtctcctc acgggcggga gaccggcgga caggcgtccc
     1861 cgcgaaggaa ccggcgaccg ggctgaccat gacctcgtcc acgtcgaacg tggcggccag
     1921 ctcacgcacg cgcgcagccg cctgctgcgg gtccccgacg acccatcggc tggccatctc
     1981 gtcggccaac ggctggtgcg ccgccacgac cccccgcgcc tccgcctcct cgacgctctc
     2041 ctgcggcatc agtggctgcc cggtgcgcag cgccaccatg gtgcgcacca tcggctgcac
     2101 gagccgctgc gcctgtgcct gcgtgggggc gacgacgacg ttgacggtga ggaaggtccg
     2161 cggctccggg tgctcgggcg agggctggta gccctcgcgg tacagggcga gcgcctcggc
     2221 ggtcccgcgg cccgagaagt ggtgcgcgaa gacgtacggc aacccgcggg acgcagcgag
     2281 ccgggccgag aagtccgagg acccgagcag ccagatcatc ggcaccgagg tggcgtgcgg
     2341 ggtggcgcga agcacgtgct caccactgcc caggcgcagg gcggccccgg cgggcgacat
     2401 catcgcgagc acgtggtcga cgtggtcggg gaagcggtcg acggcatcgg cctcgacgcc
     2461 accggcgccg ctgcgcaggg cccacgccgt gacctggtcg gtgccgggcg cgcggccgat
     2521 gccgaggtcg acgcgccccg ggaaggcggc ctcgagcagg gcgaactgct cggcgacgac
     2581 gaggggcgca tgattcggca gcatcacccc gccggagccg accctgatcc gctcggttgc
     2641 cgcggccacg atggcggcga ggaccggagg gttggtcgat gcgatgctcg gcatgttgtg
     2701 gtgctcggcc aaccagtagc ggcggtagcc gttctcgtcg gcgaccttgg ccaacgaccg
     2761 catcgcggcg acggcatcac cactgctctg gtctgctcgc acgggcacga ggtcgagcac
     2821 ggacagggcg ggcgcgggac gcatggaggg gctcaccccg tgtccagcgg tcaccggacc
     2881 actgctattc cgacggggga cctgcgggca gcggtcgctg gtgaaagacg tgccgacgca
     2941 cccacgcgtg catggcgatc gcggcggccg cgccggcgtt gatcgaccgg gtcgagccga
     3001 actgctcgat gtgcagcacg gcggtacagg ccgcccgcat cggctcgctc agccccgggc
     3061 cctcctgccc gaagacgagc acgcactcgc ggggcaggtc gtgggtctcc agcgggaccg
     3121 agccggggag gttgtcgatg ccgatgatcg ggatgccgtc ggcctgggtc cccgcgccac
     3181 tcgcccactg cacgaggtcg tcgacgtcgg ggtggtggtg ctcgtgctgg tagcggtcgg
     3241 tcaccatcgc cccgcgccgg ttccagcggc gcttgccgac gatgtggaag gccgcagcgt
     3301 tgaacgcgtt cgccgtgcgc acgaccgagc cgatgttgaa gtcgtggccc cagttctcga
     3361 tggcgacgtg gaacggg
//
LOCUS       DAOSOC010000225         3345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_28552_length_3345_cov_9.790578, whole genome shotgun sequence.
ACCESSION   DAOSOC010000225 DAOSOC010000000
VERSION     DAOSOC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28552_length_3345_cov_9.790578"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /gene="groL"
                     /locus_tag="PL014_12160"
     CDS             <1..1426
                     /gene="groL"
                     /locus_tag="PL014_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144865.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT57890.1"
                     /translation="YENLGAQLAKEVATKTNDVAGDGTTTATVLAQAMVHEGLRNVAA
                     GASPSGLKRGMDKAVEAVSDELLRTAREIESKDEIAQVASLSAQDQEIGSTIAEAFDK
                     VGKDGVITVEESSTASTELEFTEGMQFDKGYISPYFVSDPERMEAVLEDAYVLINQGK
                     ISAIADILPVLEKVVKSGKPLLIIAEDVDGEALSTLVVNKIRGTFNVVAVKAPGFGDR
                     RKAMLQDVAVLTGGQVVAEEVGLKLDQVGLETLGQARRVVVTKDTTVVIDGAGAHEDV
                     EGRVNQIKQEIERTDSDWDREKLQERLAKLAGGVCVIKVGAHTEVELKEKKHRIEDAI
                     SATRAAIEEGIVAGGGSALIQAVKVIDGLGLEGDEAVGAAIVRKAAAEPLRWIAENAG
                     MQGYVAVAKVQDLAPGMGLNAATGEYEDLVKAGVIDPVKVTRSALRNAASIASMVLTT
                     DTLVVDKKEEEPAGAGGQGHGHGH"
     gene            1561..2094
                     /locus_tag="PL014_12165"
     CDS             1561..2094
                     /locus_tag="PL014_12165"
                     /inference="COORDINATES: protein motif:HMM:NF025908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT57891.1"
                     /translation="MTTGHVVLGLLSRGQQHGYDLKRQHDELFPAVKEIAFGQVYAAL
                     GRLRDKGFVEEATRQRVDGPERTAYRITDAGRAELTSWLAQVEPLPERTANPVATKVT
                     LLLLAEGAERTVEHLVRERAVRVARMRELTAARQRPGTTFQEVLAADHALAHLDADLR
                     WIESARRRIHDLEGEIA"
     gene            2091..2810
                     /locus_tag="PL014_12170"
     CDS             2091..2810
                     /locus_tag="PL014_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57892.1"
                     /translation="MTSPVLSCRGVTHAYDGPPVLQGVDLDVLEGEIVAVTGPSGSGK
                     STLLLCLAGIIVPTSGSVHLGGVDLRALGERGRATLRRESVGIVFQYGTLVPELTGEE
                     NVALPLLLSGQPRREAIGQAREWLGRLGVDSVGATTAARMSGGERQRVALARALVVRP
                     RVVLADEPTGALDSVASDLVATELVAAARDAGAAVVLVTHDARVAAHADREVHLWDGQ
                     VDADVPLDVPGGTALESEVVS"
     gene            2807..>3345
                     /locus_tag="PL014_12175"
     CDS             2807..>3345
                     /locus_tag="PL014_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57893.1"
                     /translation="MSGTVTWGDALWLARHRTPNDRLRFWGSAVSSAITAAVLCTAIG
                     LLMIGGSTYVPVNVGVVADPGTRGGVALAVALVALPAIHLTGQAWRLGSVEQRQRFRQ
                     LRDAGAGPRELRRVAVAGACVPVFGGAVLGVLVWGAVLVLVNGGLVRGPDREGLGAIV
                     LDASKFTVQQVPNVVTDTP"
BASE COUNT          508 a         1165 c         1199 g          473 t
ORIGIN      
        1 ctacgagaac ctcggcgccc agctcgccaa ggaggtcgcc accaagacca acgacgtcgc
       61 cggtgacggc acgaccaccg cgaccgtcct cgcccaggcg atggtgcacg agggcctgcg
      121 caacgtcgcg gccggtgcgt cgccgtccgg cctcaagcgc ggcatggaca aggccgtcga
      181 ggccgtgtcc gacgagctgc tgcgcacggc ccgcgagatc gagtccaagg acgagatcgc
      241 ccaggtcgcc tcgctctccg cgcaggacca ggagatcggc tccacgatcg ccgaggcgtt
      301 cgacaaggtc ggcaaggacg gagtcatcac cgtcgaggag tcctcgaccg cgagcaccga
      361 gctcgagttc accgagggca tgcagttcga caagggctac atcagcccct acttcgtctc
      421 cgaccccgag cgcatggagg ccgtcctcga ggacgcctac gtgctcatca accagggcaa
      481 gatctcggcg atcgccgaca tcctcccggt gctcgagaag gtcgtgaagt ccggcaagcc
      541 gctgctcatc atcgccgagg acgtcgacgg cgaggccctg tcgacgctcg tcgtcaacaa
      601 gatccgcggc acgttcaacg tcgtggccgt caaggccccc ggcttcggtg accgccgcaa
      661 ggccatgctc caggacgtgg ccgtgctcac cggcggccag gtcgtcgccg aggaggtcgg
      721 cctcaagctc gaccaggtcg gtctcgagac gctgggccag gcccgccgcg tcgtcgtcac
      781 caaggacacc acggtcgtca tcgacggcgc cggtgcccac gaggacgtcg agggtcgggt
      841 caaccagatc aagcaggaga tcgagcgcac cgactccgac tgggaccgcg agaagctgca
      901 ggagcgcctc gccaagctcg ccggtggcgt ctgcgtcatc aaggtcggtg cccacaccga
      961 ggtcgagctc aaggagaaga agcaccgcat cgaggacgcc atctcggcga cccgcgcggc
     1021 catcgaggag ggcatcgtcg ccggtggcgg ctccgccctc atccaggccg tcaaggtcat
     1081 cgacgggctg ggtctcgagg gtgacgaggc cgtcggtgcc gccatcgtgc gcaaggccgc
     1141 ggccgagccg ctgcgctgga tcgccgagaa cgcgggcatg cagggctacg tcgccgtcgc
     1201 caaggtccag gacctcgcgc ccggcatggg cctgaacgcc gccaccggcg agtacgagga
     1261 cctcgtcaag gccggcgtca tcgacccggt caaggtcacc cgctcggcgc tgcgcaacgc
     1321 cgcctcgatc gcctcgatgg tgctcacgac cgacaccctc gtcgtggaca agaaggaaga
     1381 ggagccggct ggtgccggtg gccagggcca cgggcacggc cactgaggct gatcctcccc
     1441 gcgccgcctg acggcgcacc acgcacgaac cggatgccgt ccgctcaccc gagcgggcgg
     1501 catccgtctg ttccggcgtc gggcttgacg agactattca cacagtgaat agtcgagccc
     1561 atgacaacgg ggcacgtggt tctggggctg ctgtcgaggg ggcagcagca cgggtacgac
     1621 ctcaagcgcc agcacgacga gctcttcccg gccgtgaagg agatcgcctt cggccaggtc
     1681 tatgcagcgc tcgggcgcct gcgcgacaag gggttcgtcg aggaggccac gcgccagcgc
     1741 gtcgacggcc ccgagcgcac ggcctaccgg atcacggatg ccggtcgggc cgagttgacg
     1801 tcctggctgg ctcaggtcga gccactgccc gagcgcaccg ccaacccggt ggccaccaag
     1861 gtcacgctgc tgctgctggc cgagggggcg gagcgcaccg tcgagcacct cgtgcgcgag
     1921 cgcgcggtcc gcgtcgcccg gatgcgcgag ctcaccgcgg ccaggcaacg acccggaacc
     1981 accttccagg aggtcctcgc ggccgaccac gccctggcgc acctggacgc cgacctgcgc
     2041 tggatcgagt cggctcgccg gcgcatccac gacctcgagg gggaaatcgc atgacgtcac
     2101 cagtgctcag ctgccgaggt gtcacccacg cctacgacgg gccgcccgtc cttcaggggg
     2161 tcgacctcga cgtcctcgag ggggagatcg tcgccgtgac agggccgtcc ggctcgggca
     2221 agtcgaccct gctgctctgc ctggccggca tcatcgtgcc cacctcgggt tcggtgcacc
     2281 tcggcggcgt cgacctgcgc gccctggggg agcgtggtcg ggcgaccctg cgccgcgagt
     2341 cggtgggcat cgtcttccag tacggcacgc tcgtgcccga gctgaccggc gaggagaacg
     2401 tcgcgttgcc gttgctgctg tccgggcagc ctcggcgcga ggcgatcggg caggctcgcg
     2461 agtggctggg ccggctcggt gtggacagcg tcggcgcgac aacggccgcg cggatgagcg
     2521 ggggtgagag gcagcgggtc gcgctcgccc gggccctggt cgtccgacct cgcgtcgtgc
     2581 tcgccgacga acccaccggg gcgctcgact cggtggcgtc cgacctcgtc gcgaccgagc
     2641 tcgtcgccgc cgcccgggac gccggggcgg ccgtcgtgct cgtcacgcac gacgctcggg
     2701 tggcggcgca cgctgaccgc gaggtccacc tgtgggacgg gcaggtcgac gcggatgtgc
     2761 cactggacgt cccgggcggc acggcgctgg agagcgaggt ggtgtcgtga gcggcaccgt
     2821 cacgtggggt gatgccctct ggctggcccg ccatcgcacc ccgaacgacc ggctgcgttt
     2881 ctgggggagc gccgtgtcgt cggcgatcac cgccgccgtg ctgtgcacgg ccatcggact
     2941 cctgatgatc ggaggcagca cctacgttcc cgtgaacgtc ggggtggtcg cggacccggg
     3001 cactcgtggc ggcgtcgcac tcgctgtggc gctcgtcgct ctcccagcga tccacctcac
     3061 gggtcaggcg tggagactcg ggtcggtcga gcagcgccag cggttccggc agctacgcga
     3121 tgccggggcc gggccacgtg agcttcgcag ggttgccgtc gccggtgctt gtgtcccggt
     3181 gttcgggggt gccgtgctgg gcgtgctcgt gtggggggcg gtgctggtgc tcgtcaacgg
     3241 cggattggtg cgcggcccgg accgagaggg cttgggggcg atcgtcctcg atgcctcgaa
     3301 attcacagtt caacaggtgc cgaacgtcgt aacggataca ccatg
//
LOCUS       DAOSOC010000226         3344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_28562_length_3344_cov_9.363028, whole genome shotgun sequence.
ACCESSION   DAOSOC010000226 DAOSOC010000000
VERSION     DAOSOC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28562_length_3344_cov_9.363028"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /gene="coaA"
                     /locus_tag="PL014_12180"
     CDS             <1..683
                     /gene="coaA"
                     /locus_tag="PL014_12180"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HOT57894.1"
                     /translation="VGVAGSVAVGKSTTARILKELMSRWPQTPRVELVTTDGFLYPNA
                     ELERRGLLQRKGFPESYDRRALMRFIADVKSGMPSVQAPMYSHLTYDVLPGSVSVRRP
                     DVLIVEGLNVLQPPQVRPDGTSTGAISDYFDFSVYVDAPVEEIRTWYVQRFLRLRETA
                     FADPKSYFHRYAALSDDEAVATARRIWEQINEPNLVENVLPTRDRATLVLSKGADHAV
                     TGIRLRKI"
     gene            complement(687..1421)
                     /locus_tag="PL014_12185"
     CDS             complement(687..1421)
                     /locus_tag="PL014_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57895.1"
                     /translation="MARYTARHAPRHAAARPERSGLRRALAATGARPVLASGAVAAVL
                     AGGALAVSVGESQARAEAMTFAVDGLTEQTSTEQLAAEEEDAARVATARTDTNAARSA
                     VAAREAERARLAAAKALAARQAKAKAAARAAERKRIVADAKKDPKAAARLLMPEYGFG
                     QGQWSCLERLWIGESGWKWNAENPSSGAYGIPQSLPARKMAAMGDDYRTNPVTQIRWG
                     LDYIKKAYGTPCNALSKWNSRSPHWY"
     gene            complement(1584..2921)
                     /locus_tag="PL014_12190"
     CDS             complement(1584..2921)
                     /locus_tag="PL014_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT57896.1"
                     /translation="MASSTRPAPSARQGEEPRRTYVLDTSVLLSDPRAITRFKEHEVV
                     LPVVVVTELEAKRHHPELGYFARTSLRMLDDLRISAGRLDAPVPIGDQGGTVRIELNH
                     TDAASLPAGFRLGDNDTRILAVARNLANEGRLVTIVSKDLPMRVKASACGLEAEEYRA
                     ELAVESGWTGMAELEVTVEEMDHLYEYGRLESVAAAEFPCHTGLTVLSPRGSALGRVG
                     PDKQVRLVRGDRDAFGLHGRSAEQRIALDLLMDPDIGIVSLGGRAGTGKSALALCAGI
                     EAVMERRQQRKVVVFRPLYAVGGQELGYLPGDAAEKMNPWGQAVFDTLSAVVSREVVE
                     EILDRGMLEVLPLTHIRGRSLHDAFVIVDEAQSLERNVLLTVLSRIGQNSRVVLTHDV
                     AQRDNLRVGRHDGVVAVVEALKGHPLFAHVTLTRSERSPIAALVTDLLEGIDV"
     gene            complement(3084..>3344)
                     /locus_tag="PL014_12195"
     CDS             complement(3084..>3344)
                     /locus_tag="PL014_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOT57897.1"
                     /translation="AMGTSIEELAEVIDVEHIAEHLYTKGQPDPDLVIRTSGEQRLGG
                     FLLWQSAHSEFYFCEAYWPDFRKVDFLRALRAYGERHRRFGS"
BASE COUNT          475 a         1154 c         1197 g          518 t
ORIGIN      
        1 tcgtcggcgt ggccgggtcg gtggcggtcg gcaagtcgac gacggcccgc atcctcaagg
       61 agctgatgag tcgctggccg cagacgccac gcgtcgagct cgtcaccacc gacggcttcc
      121 tctacccgaa cgccgagctc gagcgtcggg gcctgctgca gcgcaagggc ttcccggagt
      181 cctacgaccg ccgcgcgctc atgaggttca tcgccgacgt gaagtcaggg atgccgtccg
      241 tccaggctcc gatgtactcg cacctcacgt acgacgtcct ccccggcagc gtgagcgtgc
      301 ggcgccccga cgtgctcatc gtcgagggac tcaacgtcct gcagcctcca caggtccgcc
      361 cggacggcac gagcaccggc gcgatctcgg actacttcga cttctcggtg tacgtcgacg
      421 cacccgtcga ggagatccgc acctggtacg tgcaacggtt cctccgcctg cgggagaccg
      481 cgttcgccga ccccaagtcc tacttccacc gctacgccgc tctcagcgac gacgaggcgg
      541 tcgccacggc gcgccggatc tgggagcaga tcaacgagcc gaacctcgtc gagaacgtcc
      601 tgccgacccg ggaccgcgcg acgctcgtgc tgagcaaggg cgccgaccac gcggtcaccg
      661 gcatccggtt gcgcaagatc tgacgatcag taccagtgcg gggagcggct gttccacttg
      721 ctgagggcgt tgcacggggt gccgtacgcc ttcttgatgt agtccagccc ccaccggatc
      781 tgggtcaccg ggttggtgcg gtagtcatcg cccatggccg ccatcttgcg ggccgggagg
      841 gactgcggga tgccgtaggc gccggatgac gggttctcgg cgttccactt ccagccgctc
      901 tcaccgatcc acaggcgctc gaggcaggac cactggccct gaccgaagcc gtactccggc
      961 atgagcagcc gagcagcggc cttcgggtcc ttcttggcgt cggcgacgat gcgcttgcgc
     1021 tcggcagcgc gtgcggccgc cttcgccttg gcctgacgtg cggccagggc cttcgccgct
     1081 gcgaggcgtg ctcgctcggc ctcacgggcc gcgacggccg agcgggccgc gttggtgtcg
     1141 gtgcgcgcag tcgcgacgcg agcggcatcc tcctcctcgg cggcgagctg ctccgtcgag
     1201 gtctgctcgg tgagcccgtc gaccgcgaag gtcatcgcct cggcgcgggc ctggctctcc
     1261 ccgaccgaga ccgcgagggc acctccggcg agcaccgcag ccactgcccc gctcgccagc
     1321 accggtcgcg caccggtcgc tgcgagagcg cggcgcagcc cgctgcgctc ggggcgcgcg
     1381 gcggcatggc gaggcgcgtg acgcgcggtg tagcgagcca tgggcaggca ggggacctct
     1441 cgtgcgggag atgctcaggg ggggctggac gcagccggtg gagcactcac gtcgttaccg
     1501 aatcgagaca caaggttacg gggtcaggcg gcgttgggtg aaatcgcctg ttcagacgtc
     1561 gatgtgctca gacgtcgatg tgctcagacg tcgatgccct cgaggaggtc ggtgacgagc
     1621 gcggcgatgg ggctgcgctc ggaccgcgtg agcgtcacgt gggcgaagag cgggtgaccc
     1681 ttgagcgcct ccaccaccgc gacgaccccg tcgtgacggc ccacccgcag gttgtcgcgc
     1741 tgggcgacgt cgtgggtcag cacgacgcgg gagttctgcc cgatgcgcga gagcacggtg
     1801 agcagcacgt tgcgctccag gctctgggcc tcgtcgacga tgacgaacgc gtcgtgcagg
     1861 gaccggcccc tgatgtgggt caggggcagc acctcgagca tcccgcggtc gaggatctcc
     1921 tcgaccacct cgcgcgacac caccgcggag agggtgtcga agacggcctg cccccacggg
     1981 ttcatcttct cggcggcgtc accgggcagg tagccgagct cctgcccacc caccgcgtac
     2041 agcggccgga agacgacgac cttgcgctgc tggcgccgct ccatgacggc ctcgatcccg
     2101 gcgcacaggg cgagggctga cttgcccgtg ccggcacggc caccgaggct gacgatgccg
     2161 atgtcggggt ccatgagcag gtcgagggcg atgcgttgct cggcgctgcg accgtgcagg
     2221 ccgaacgcgt cacggtcacc cctcaccagg cgcacctgct tgtcgggccc cacgcgcccc
     2281 agcgccgagc cgcggggcga gagcacggtg agcccggtgt ggcaggggaa ctcggccgcg
     2341 gcgacgctct cgagccggcc gtactcgtag aggtggtcca tctcctcgac ggtgacctcg
     2401 agctcggcca tgccggtcca ccccgactcg accgcgagct cggcccggta ctcctccgcc
     2461 tcgaggccgc acgccgaggc cttgacccgc atcggcaggt ccttgctcac gatcgtgacg
     2521 agccgcccct cgttggccag gttgcgcgcc accgcgagga tgcgggtgtc gttgtcgccg
     2581 aggcgaaagc ccgcgggcaa cgacgcggca tccgtgtggt tgagctcgat gcgcacggtg
     2641 ccgccctggt cgccgatcgg cacgggagcg tcgagccgac cggcggagat ccgcaggtcg
     2701 tcgagcatcc gcagggacgt gcgggcgaag tagccgagct cggggtggtg gcgcttggcc
     2761 tcgagttcgg tgacgacgac caccggcagc accacctcgt gctccttgaa gcgggtgatc
     2821 gcgcgcgggt cggacagcag caccgaggtg tcgagcacgt acgtgcgacg cggctcctcc
     2881 ccctggcgtg ctgacggtgc cggtcgggta ctcgaggcca tggtcgctcc tgaggggtgc
     2941 ggggctgcag tgctctcgac gctacgactg cctcccccac gcccccacgc acgcggccgg
     3001 gcgtgttgag gtgaattctg cgtcatgtcg tgacgggtgt ggctggtgtg gctcgtgtga
     3061 cgtttgggga tgccgggggc gtgtcaggaa ccgaacctgc ggtgccgctc gccgtaggcg
     3121 cgcagggcgc gcaggaagtc gaccttgcgg aagtcgggcc agtaggcctc gcagaagtag
     3181 aactcgctgt gcgcgctctg ccagagcagg aacccgccga gccgctgctc ccctgacgtg
     3241 cggatcacga ggtccgggtc gggctgaccc ttggtgtaga ggtgttcggc gatgtgctcg
     3301 acgtcgatga cctcggcgag ctcctcgatg ctcgtcccca tggc
//
LOCUS       DAOSOC010000227         3341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_28600_length_3341_cov_6.519172, whole genome shotgun sequence.
ACCESSION   DAOSOC010000227 DAOSOC010000000
VERSION     DAOSOC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28600_length_3341_cov_6.519172"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="PL014_12200"
     CDS             <1..1229
                     /locus_tag="PL014_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOT57898.1"
                     /translation="KVDGQYHGPVVWVKDASRSVPVVSQLLSDEQRGAFLDGVSADFD
                     AIRARHAAKAGERPLASLEQARANRPVLDWASYRPMRPHLLAQQSADRWSGRPDAHWH
                     RAATQFTRVFEDFPLAELRPYIDWTPFFGAWEIKGRYPDVLNNAATSEQARSLLADAN
                     AMLDRMERERWVHARGVVGIFPANGVGDDIEVYADEARTTVLTTLRGLRQQGQHREGV
                     SNKCLSDFIAPKETGIADHVGAFSVTAGLGLEERLLAFKADLDDYSAILLESLADRLA
                     EAFAERLHEKVRRELWGYAPDEHLSMDDLIHERYAGIRPAPGYPACPEHTEKETLMAL
                     LGGEERTGIRLTESMAMWPGASVSGWYFAHPDSQYFVVGRLGRDQVESYAERKGWDLR
                     TAERWLGPNLGYQPED"
     gene            1289..2134
                     /locus_tag="PL014_12205"
     CDS             1289..2134
                     /locus_tag="PL014_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT57899.1"
                     /translation="MTPALSPSRAADFKQCPLLYRFRTIDKLEGPPSPAAARGTLVHA
                     VLEHLFDLPAEQRTPEAALALVEPRWQALVEERPELATMIDDDEQLTEQAWFAAAGAL
                     VEQWFTLEDPTRLQPAARELYVEAEVEGLTLRGYVDRLDVAPDGAMRVVDYKTGRSPS
                     ELFEAKALFQMKFYALVLWRIHGTIPALLQLVYLGNGEVVRYSPDERDLLAVERNVRA
                     IWDAVANAARTGDWRPRTSKLCDWCDFKDLCPAWGGTPPPLPEGAGLLALDPARSGEA
                     VPADD"
     gene            2401..2559
                     /locus_tag="PL014_12210"
     CDS             2401..2559
                     /locus_tag="PL014_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57900.1"
                     /translation="MRRPGLLRRLLGALGRLVADSPPSDRPPGSNYLPNSNGEGGRVI
                     SSGDGNSS"
     gene            complement(2566..3240)
                     /locus_tag="PL014_12215"
     CDS             complement(2566..3240)
                     /locus_tag="PL014_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT57901.1"
                     /translation="MSLPAAVLWDMDGTLVDTEPYWIAAEHDIVEEHGGTWTEELAHQ
                     LVGQDLLVAATFIRDNSAVDWSPERIVDEMLGRVIGKVREHIPWRPGARELLEALKAE
                     GVPSALVTMSWRSLADAVLGALPEGTFDVVVTGDEVSHGKPHPEPYRAAARLLGVSPQ
                     DCVAIEDSPTGVRSAVAAGVPTLAVPHVVSVPEMAGAVHLDSLAGLTPSRVAELGRQA
                     RASAVA"
BASE COUNT          483 a         1179 c         1194 g          485 t
ORIGIN      
        1 tcaaggtcga cgggcagtac cacgggccgg tggtctgggt gaaggacgcg tcgcgctcgg
       61 tgcccgtggt cagccagctg ctgtccgacg agcagcgggg tgccttcctc gacggcgtga
      121 gcgccgactt cgacgcgatc cgggcccggc acgccgccaa ggccggtgag cggccgctgg
      181 cctccctgga gcaggcccgg gcgaaccggc ccgtgctcga ctgggcctcc taccgtccga
      241 tgcggcccca cctgctggcg cagcagagcg ccgaccggtg gtccggtcgt cccgatgcac
      301 actggcaccg ggccgcgacc cagttcaccc gggtcttcga ggacttcccc ctggccgagc
      361 tgcggcccta cattgactgg acgccgttct tcggggcctg ggagatcaag ggccgctacc
      421 ccgacgtgtt gaacaacgcc gcgacgtcgg agcaggcgcg gtcgctgctg gccgacgcca
      481 acgcgatgct cgaccggatg gagcgcgagc gctgggtcca cgctcgcggg gtggtcggga
      541 tcttcccggc gaacggggtg ggcgacgaca tcgaggtcta tgccgacgag gcacgcacca
      601 ccgtgctcac caccctgcgc gggctgcgtc agcagggtca gcaccgtgag ggggtgtcga
      661 acaagtgcct gtccgacttc atcgccccga aggagaccgg tatcgccgac cacgtcggcg
      721 ccttctccgt gaccgccggg ttggggttgg aggagcgtct cctggcgttc aaggccgacc
      781 tggacgacta ctcggcgatc ctgctcgagt cgttggcaga ccgccttgcc gaggcctttg
      841 ccgagcgcct gcacgagaag gttcgccgtg agctgtgggg gtacgcaccc gacgagcacc
      901 tgtcgatgga cgacctgatc cacgagcgct acgcgggcat ccgaccagcg ccgggatacc
      961 cggcctgccc cgagcacacc gagaaggaga cgctcatggc gctgctcggt ggggaggagc
     1021 gcaccggcat ccggctcacc gagtcgatgg cgatgtggcc gggggcttcg gtgtcgggct
     1081 ggtacttcgc tcaccctgac agccagtact tcgtcgtcgg ccggctcggg cgcgaccagg
     1141 tggagtcgta tgccgagcgc aagggctggg acctgcgcac cgccgagcgc tggctgggcc
     1201 cgaacctggg gtaccagccc gaggactgat ccccgaggct gtggatgccg tggcaccggt
     1261 gtcggtgggt cggcatagcg tgtgccccat gactcccgca ctgtccccga gccgcgccgc
     1321 ggacttcaag cagtgccccc tgctgtaccg cttccgcacg atcgacaagc tggagggtcc
     1381 gccctcccct gctgcggccc gcggcacgtt ggtgcacgcg gtgctcgagc acctcttcga
     1441 ccttcccgcc gagcagcgca cgcccgaggc ggcgttggcg ctggtcgaac cgcggtggca
     1501 ggcgctcgtc gaggagcggc ccgagctcgc gacgatgatc gacgacgacg agcagctcac
     1561 cgagcaggcc tggttcgcgg ccgccggcgc actggtcgag cagtggttca ccctcgagga
     1621 cccgacccgc ctccagcccg ccgcgcgcga gctgtacgtc gaggccgagg tggaggggct
     1681 caccctgcgc gggtacgtcg accggctcga cgtcgccccg gacggggcca tgcgggtcgt
     1741 cgactacaag accggccgat cgcccagcga gctcttcgag gccaaggccc tgttccagat
     1801 gaagttctat gcgctggtgc tgtggcgcat ccacggcacc attccggcgc tgctccagct
     1861 cgtctacctc ggcaacggcg aggtggtgcg ctactcccct gacgagcgcg acctgctcgc
     1921 ggtcgagcgc aacgtgcggg ccatctggga tgccgtggcg aacgccgcgc gcaccggcga
     1981 ctggcgtccg cgcacctcga agctgtgtga ctggtgcgac ttcaaggacc tgtgccccgc
     2041 gtggggcggt actcctcccc cgcttcccga gggcgctggc ctgctcgcgc tcgaccccgc
     2101 gcgttccggt gaggccgttc ctgccgacga ctgacggccg ggctcgcgga gttcgccgct
     2161 ggtagaatta ctcatcggcg ccacagcgcg ccgggggaag gcaggtcgcc atgaggtgct
     2221 cgaagtgccg gatggaagcc agctccacgg ggccgagcgg cgaacggctg tgcgaccgcc
     2281 actacaaccc caccatggcc gctgctccgg ggatggtgca cggcggctga tgccgtcggc
     2341 ggcttcatcg tggccgaggg tgtcgccacc caggtgcacg gttctcacgc tccgacaccc
     2401 gtgaggcgcc cgggcctgct gcggcgcctg ctgggcgcct tggggcggct cgtggccgac
     2461 agcccgccct cggaccgccc gccgggcagc aactacctgc cgaacagcaa cggcgagggc
     2521 ggccgagtca tctccagcgg ggacggcaac tcgtcctgag gctggtcagg caacggccga
     2581 agcgcgagcc tgacgaccga gctcggccac ccggctcggg gtgagcccgg ccagcgagtc
     2641 caggtgcacc gcgcccgcca tctcgggcac ggagacgacg tgcggcacgg ccagcgtcgg
     2701 cacgcccgcg gcgacagctg accgcacccc ggtcggtgag tcctcgatcg ccacgcagtc
     2761 ctgcggcgac acgccgagca ggcgagcagc agcccggtac ggctccgggt gcggcttgcc
     2821 gtgcgacacc tcgtcgccgg tgaccacgac gtcgaaggtg ccctcgggca gggcgcccag
     2881 cacggcatcc gccaacgagc gccacgacat ggtgaccagc gcgctcggga ccccctcggc
     2941 cttgagggcc tccagcagct cacgggcgcc cggccgccac gggatgtgct cgcggacctt
     3001 gccgatgacc cgaccgagca tctcgtcgac gatccgctcg ggagaccagt cgacggcgga
     3061 gttgtcccgg atgaacgtcg ccgcgacgag gaggtcctgg ccgacgagct ggtgggcgag
     3121 ctcctcggtc cacgtcccgc cgtgttcctc gacgatgtcg tgctccgcgg cgatccagta
     3181 gggctcggtg tcgacgaggg ttccgtccat gtcccagagg actgctgcgg ggaggctcat
     3241 gagacatcag tctacggagc cgtagggtgg tgctccgacg acgaggaggc cccggtgatc
     3301 gagctcgacg acctgcccga gctgaaggac gcggtgatga t
//
LOCUS       DAOSOC010000228         3340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_28610_length_3340_cov_8.847793, whole genome shotgun sequence.
ACCESSION   DAOSOC010000228 DAOSOC010000000
VERSION     DAOSOC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28610_length_3340_cov_8.847793"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1303)
                     /locus_tag="PL014_12220"
     CDS             complement(134..1303)
                     /locus_tag="PL014_12220"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOT57902.1"
                     /translation="MRVLIAPDSFGSTLGPVQAATAMAAGWAEGAPHDEVEVLPLSDG
                     GPGFLDVLMRALGGTTIATTVSDPLGREVPAAVLVVERDGRRTAYVEASQASGLHLLG
                     ADERDPSITGTWGVGQLLEAALATGPDKVVVGVGGSGTHDAGAGMLSALGAGPASELA
                     RGGRHLATVSDDVLLGLPGVIDRWRGVDLVLATDDDTPLLGLQGTSAVDAPRKGASPE
                     QAQALEAALGRFTEVVGRALPTPQVDLLSGMPWRLDREPGAGAAGGLGYALLALGARR
                     ASAVGEVLRECGFGVAAARSDLVVTGAGLVDWRQARDSVVAGVAAATMETARPTVLIA
                     GECLLGRRDTMTMGFASSYAVAETPAELEAMVADPVATLAQRTARVARTWSPAAP"
     gene            1335..2528
                     /gene="nadA"
                     /locus_tag="PL014_12225"
     CDS             1335..2528
                     /gene="nadA"
                     /locus_tag="PL014_12225"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOT57903.1"
                     /translation="MTTTTGPAHARLPLLVLGAGRDLASERGVECPGDLPAPSDPDLV
                     ARARAAKAALGDRVFVLGHHYQRDEVIEFADVTGDSFKLAKEAAARPDAEWIVFCGVH
                     FMAESADILTSDAQTVILPDLAAGCSMADMAAIDQVEECWDELVEAGVAEATVPVTYM
                     NSSAAIKAFTGRHGGTICTSSNAKTALTWALEKAGDGGKVLFLPDQHLGRNTYARDLG
                     GSLDDCVVWDPHRPMGGLTPEQLRDATMILWRGHCSVHGRFTVDAVEAARRTIPGVKV
                     IVHPECRNEVVELADEVGSTEKIIKTIAAAPAGTAWAVGTELNLVQRLAAQFPEQQIA
                     FLEKNVCYCSTMNRIDLPHLVWALESLVEGRVVNTIRVDDQVAHDARVALDQMLALPG
                     ETSKD"
     gene            complement(2576..3121)
                     /locus_tag="PL014_12230"
     CDS             complement(2576..3121)
                     /locus_tag="PL014_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57904.1"
                     /translation="MSDTIADRVGRLILDGSDQENVDHLELVARAAEAHEATRDLLHQ
                     SVATARAGGHSWAAIGAVLGLTRQAAQQRFGGAVDKPGGVDQPMDAGGLLGAEVRWLG
                     PVTAFDEMAELALAGRRGWRTVDAGMLKHKVVRTPTQWEHRRVAWTRPASALEQDGWH
                     VAVRAFPWLYLVRDTGIPAED"
     gene            complement(3114..>3340)
                     /locus_tag="PL014_12235"
     CDS             complement(3114..>3340)
                     /locus_tag="PL014_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57905.1"
                     /translation="GIPTLGMSLVLLGLVTGALSRVVESGFFHGLFQGMTIALMALGA
                     YAIGRGVWGSRGDGDEPQGSMWLPSRDRRE"
BASE COUNT          478 a         1233 c         1185 g          444 t
ORIGIN      
        1 ctagtagtgc gacgatcccg gtgagaagtc gaaccttcat gtcgataccc cttcgatttc
       61 gtcgagtggt accccctccc gactggtgat cgtgactcta gggcagccct gaggtcgcag
      121 ccaccggagt aggtcagggg gccgccggtg accaggtgcg ggcgactcgc gccgtgcgct
      181 gcgccaacgt ggccacgggg tcggcaacca tggcctccag ctcggcgggc gtctccgcga
      241 cggcgtagct gctggcaaaa cccatggtca tcgtgtcgcg acgaccgagc aggcactccc
      301 ctgcgatgag aacggtcggc cgggccgtct ccatcgtcgc tgcggcgacc ccggcgacca
      361 ccgagtcgcg ggcctgtcgc cagtcgacga ggccggcacc ggtcacgacc aggtcgctgc
      421 gggccgcggc caccccgaag ccgcactccc gcagcacctc tcccacggca ctggcacgcc
      481 gcgcacccag ggcgagcagg gcgtacccga ggccaccggc cgcaccggcg ccgggttcgc
      541 ggtccaggcg ccacggcatc ccactcagga ggtcgacctg gggggtcggc agtgcgcggc
      601 cgaccacttc ggtgaaccgg cccagggccg cctcgagggc ctgcgcctgt tccgggctcg
      661 cccccttgcg cggagcgtcg acggcgctgg tgccctggag gccgagcagc ggcgtgtcgt
      721 cgtcggtcgc caggacgagg tcgaccccgc gccaccggtc gatgacgccc ggcaggccga
      781 ggaggacgtc gtcggacacc gtggcgaggt ggcggccacc gcgggcgagt tcggatgccg
      841 gtcccgcgcc gagcgctgag agcatccccg ccccggcgtc gtgggttccg ctgccgccca
      901 ccccgacgac gaccttgtcg ggcccggtcg ccagggccgc ctccagcaac tgcccgaccc
      961 cccaggtgcc ggtgatggag gggtcgcgct cgtccgcgcc gagcaggtgc agaccgctgg
     1021 cctgggatgc ctcgacgtat gccgtgcgcc tgccgtcgcg ctcgacgacc agcaccgcgg
     1081 cgggcacctc acggccgagc gggtcgctca cggtcgtcgc gatggtggtg ccaccgaggg
     1141 cccgcatgag cacgtcgagg aaccccgggc caccgtccga gagcggcagc acctcgacct
     1201 cgtcgtgcgg cgcaccctcg gcccagccgg cagccatggc cgtggctgcc tggaccgggc
     1261 ccagggtcga accgaaggag tcgggggcga tcagcacacg cacgggtcga ggttatcgcc
     1321 ctacgatcga cctcgtgacg acgacgaccg gtcctgccca cgcccgactg cccctgctcg
     1381 tgctcggcgc gggccgtgac ctcgcgagcg aacgcggtgt cgagtgcccg ggcgacctcc
     1441 cggccccgtc ggaccccgac ctcgtggcgc gcgcccgggc ggcgaaggcg gccctcgggg
     1501 accgggtctt cgtgctcggt caccactacc agcgggacga ggtcatcgag ttcgccgacg
     1561 tcacgggtga ctcgttcaag ctggccaagg aggcagcggc ccgcccggac gcggagtgga
     1621 tcgtgttctg cggcgtgcac ttcatggccg agtccgccga catcctcacc tccgacgcgc
     1681 agaccgtgat cctgcccgac ctcgccgccg ggtgctcgat ggctgacatg gccgcgatcg
     1741 accaggtcga ggagtgctgg gacgagctcg tcgaggccgg tgtggccgag gcgaccgtgc
     1801 cggtgacgta catgaactcc tccgcggcga tcaaggcgtt cacgggtcgc cacggcggca
     1861 cgatctgcac ctcgtcgaac gcgaagaccg ccctcacctg ggcgctcgag aaggccggcg
     1921 acggcgggaa ggtgctgttc ctgcccgacc agcacctggg ccgcaacacc tacgcccgcg
     1981 acctcggcgg gtcgctggac gactgcgtgg tctgggatcc gcaccgcccc atgggcgggc
     2041 tcacccctga gcagcttcgc gacgcgacga tgatcctctg gcgtggtcac tgctcggtgc
     2101 acgggaggtt caccgtggat gccgtggagg ccgcccgccg cacgatcccc ggcgtcaagg
     2161 tgatcgtgca ccccgagtgc cgcaacgagg tcgtcgagct cgccgacgag gttggctcga
     2221 ccgagaagat catcaagacc atcgcggcag cacccgccgg cacggcgtgg gcggtcggca
     2281 ccgagctcaa cctcgtgcag cgcctggctg cgcagttccc cgagcagcag atcgccttcc
     2341 tcgagaagaa cgtctgctac tgctcgacga tgaaccggat cgacctgccg cacctcgtgt
     2401 gggccctgga gtccctcgtc gagggtcgcg tcgtcaacac catccgggtc gacgaccagg
     2461 tcgcccacga cgctcgggtg gcgctggacc agatgctcgc cctgcccggc gagacctcga
     2521 aggactgacc cgcgacgctc gacgagggcg tcgcggaccg ggcaccgccg cccggtcagt
     2581 cctcggcggg gatgccggtg tcccggacga ggtagagcca cgggaaggcc cgcacggcga
     2641 cgtgccaccc gtcctgctcg agggcggagg ccgggcgggt ccaggccacg cgacggtgct
     2701 cccactgcgt cggggtgcgc acgaccttgt gcttgagcat ccccgcgtcg accgttcgcc
     2761 acccccggcg gccggcgagg gcgagctcgg ccatctcgtc gaaggcggtc accgggccca
     2821 gccagcgcac ctcggccccg agcagaccgc cggcgtccat gggctggtcg acaccgcccg
     2881 gcttgtccac ggcgccgccg aagcgttgct gtgcggcctg gcgcgtcagg ccgaggaccg
     2941 ccccgatcgc cgcccagctg tggccgcccg cgcgggccgt cgcgaccgac tggtggagca
     3001 ggtcgcgggt cgcctcgtga gcctcggcgg cccgagccac gagctcgagg tggtcgacgt
     3061 tctcctggtc gctgccgtcg aggatgagcc gaccgacgcg gtcagcgatc gtgtcactca
     3121 cgacggtcac ggctcggcag ccacatcgac ccctgcggct cgtccccgtc gccgcggctc
     3181 ccccacaccc cacgaccgat cgcgtaggca ccgagcgcca tgagggcaat ggtcatccct
     3241 tggaagagac cgtggaagaa cccggactcg accactcggc tgagagcgcc ggtgacgagg
     3301 ccgaggagca cgaggctcat gccgagggtg gggatgccgg
//
LOCUS       DAOSOC010000229         3330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_28712_length_3330_cov_10.657099, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000229 DAOSOC010000000
VERSION     DAOSOC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28712_length_3330_cov_10.657099"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..371
                     /locus_tag="PL014_12240"
                     /pseudo
     CDS             1..371
                     /locus_tag="PL014_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013603143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family peptidase"
     gene            765..1268
                     /locus_tag="PL014_12245"
     CDS             765..1268
                     /locus_tag="PL014_12245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT57906.1"
                     /translation="MDHVHLSLTGDGSSTIPGCGPGGLPGEVALSGWAAPAKGPITSD
                     FGPRNSPGGIGSTYHRGIDLGGGGCYGPIWAANKGQVVTAGPSSGYGNLIEIDHGGGV
                     HTRYGHMFNDGLMVKIGDQVTAGQQIAKVGTTGNSTGCHLHFEVLMNGQQVDPEAFLA
                     KVNVTIK"
     gene            1280..2608
                     /locus_tag="PL014_12250"
     CDS             1280..2608
                     /locus_tag="PL014_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOT57907.1"
                     /translation="MQNTATVRPDGTGVVTIDGQEPTRITGHSVEDTRRALLGHVIEH
                     ARRTGQHVDVLARDPEAEHRLRVHPDGRVQPLPAQVPPAPATPVPQPTTTATPPVQIQ
                     PAPQPTPNGTASVPTPAPRREASPMTQTEQPIQTRREARERSFLADARMSAPPPTGWQ
                     AFLARLGIKSGPSPEEISKWEDERAISQHWPGPRTIAVLNGKGGAGKTPATALLSAMF
                     ARAGGSGVLAWDNNVTRGTLGWRTESGPHEATVLDLIPRTDELLTPGARAADLAAVVH
                     HQTIDKYDVLRSNPLLLSTEQKLTPDQLDAVHAVAAKYYRLIFMDSGNDEGDALWLRM
                     IDHADQIVVATSTRPDHAEAGRLLLNALADRDEHSARLADQAVVLVSQADREEADASS
                     IARGFDALARAVVTVPYDPAMRQQWLRVDNLAAPTQRAYLRAAAAVAAGL"
     gene            3112..>3330
                     /locus_tag="PL014_12255"
     CDS             3112..>3330
                     /locus_tag="PL014_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57908.1"
                     /translation="MSRVANESDAAGTPLPRARRYYRPCTGPRDVRSTIARSCRGRAW
                     RPANGPTLPATVPVWSSAAHWLETLAAAL"
BASE COUNT          577 a         1280 c         1062 g          411 t
ORIGIN      
        1 accacgagca gctcgtcaac gccgcgcaca tcatgctcgc cgcgcagaag ctcgggctca
       61 gcgcccgaga ccagcagatc ggcgtcatga ccgcgatggg cgagtccagc ctgcgcgtgc
      121 tggaccgagg cgacgccgtg ggacccgaca gccgcgggct gttccagcag cgcgacaacg
      181 gcgcgtgggg ctcctacgag gaccggatga acccgttcat ctcggccacg aacttcttca
      241 aggtcgagat gacgatcgcg ggccgcgaag ccatggagcc cacgctcgtc gccaacgcgg
      301 tccagcgcaa cgctgacccg tactactaca cgccgttctg ggcaccagcc ggtgaagtca
      361 ccaaggccct gggcgggatc aaggagcagg ccggggccac ggccaccagc accgccggaa
      421 cgccgcagtc gtcctacgcg ctcgggcccg tgaagccgca gaccgccacc gtggcgaaca
      481 cggtcgggcc gatgttcggc atcaagaccg ctggcggcta ccgcgatccg caggccgagc
      541 agtacgaccc caacggccac ccggccgggc tcgcgatcga cttcatgacc aacgacattc
      601 ccgatggcaa ggccaccggg gacaagctgg ccaagtacct gcaagaccac gcggccgacc
      661 tcggcgtgaa gtacatcatc tggcagcagc acatctggtc ggtcgagcga gccgacgagg
      721 gctggcggcc gatggccgac cgaggatcgc ccacgcagaa ccacatggat cacgtgcacc
      781 tgtcgctgac cggcgacgga tcgagcacca tccccggatg cgggccgggc gggctgcccg
      841 gcgaggtggc cctgtccggt tgggccgccc cggccaaagg cccgatcacc tcggacttcg
      901 ggccgcgcaa ctcccccggc gggatcgggt cgacctatca ccgaggcatc gacctcggcg
      961 gcggcggctg ctacggcccc atctgggccg ccaacaaggg ccaggtcgtg accgctggcc
     1021 cgtccagcgg ctacggcaac ctgatcgaaa tcgaccacgg cggcggggta cacacccgct
     1081 acggccacat gttcaacgac ggcctcatgg tcaagatcgg ggatcaggtc accgccgggc
     1141 agcagatcgc caaggtcggc acgaccggca acagcaccgg atgccatctg cacttcgagg
     1201 tgctgatgaa cggccagcaa gtagaccccg aggccttcct ggccaaggtc aacgtcacca
     1261 tcaagtgagg gagagaccca tgcagaacac cgcgaccgtc cgcccggatg ggactggcgt
     1321 cgtgaccatc gacggccagg agcccacccg catcaccggg cacagcgtcg aggacacccg
     1381 ccgagcactg ctcgggcacg tcatcgagca cgcccgccgc accgggcagc acgtcgacgt
     1441 cctggcccgc gacccggagg ccgagcaccg actgcgcgtc caccctgacg ggcgcgtgca
     1501 gccgcttccc gcgcaggtgc cgcccgcacc agcgacccct gtgccgcagc cgaccaccac
     1561 cgctacgccg ccggttcaga ttcagccggc cccgcagccc acgcccaacg gcaccgcctc
     1621 ggtgccgaca cccgcgccca ggagagaggc atcccccatg acccagaccg agcagcccat
     1681 ccagacccgc cgagaggccc gtgaacggtc cttcctggcc gacgcgcgca tgagcgcgcc
     1741 gccgcccacg ggctggcagg ccttcctcgc ccgcctcggg atcaagtctg gtccgagccc
     1801 cgaggagatc tccaagtggg aggacgagcg ggccatctcc cagcactggc ccggcccgcg
     1861 cacgatcgcc gttctcaacg gcaagggcgg cgcgggcaag accccggcca ccgccctgct
     1921 cagcgccatg ttcgcccgcg ccggaggctc aggggtgctc gcgtgggaca acaacgtcac
     1981 gcgcggcacc ctcgggtggc gcaccgagag cggcccccac gaggcgaccg tgctcgacct
     2041 catcccgcgc accgacgagc tgctgacccc tggcgcacgg gcggccgacc tggccgccgt
     2101 cgtgcaccac cagacgatcg acaagtacga cgtgctgcgc tcgaacccgc tcctgctctc
     2161 gaccgagcag aagctcaccc cggaccagct cgacgccgtg cacgccgtcg ccgcgaagta
     2221 ctaccggctg atcttcatgg actccggcaa cgacgaaggc gacgccctgt ggctacggat
     2281 gatcgaccac gccgaccaga tcgtcgtggc cacctccacc cggccggacc acgccgaagc
     2341 cggacggctc ctgctcaacg ccctggccga ccgcgacgag cactccgccc gtctcgcgga
     2401 ccaggccgtc gtgctcgtct cccaggccga ccgcgaggaa gccgacgcct cctccatcgc
     2461 ccgcggcttc gacgccctcg cccgcgccgt cgtcaccgtc ccctacgacc cagccatgcg
     2521 ccaacagtgg ctgcgcgtcg acaacctcgc cgcacccacc cagcgcgcct acctgcgcgc
     2581 ggcggccgcc gtggcagcag gcctgtgatc accggtcaca cgggcaacac gccggtgaag
     2641 gccaacttcc ccccgattct gatacgaggg tgtttggatg ggtctatgcc gagagccagc
     2701 aagggtgaca gagacatcat ggtcacgcgc cctgcccgcg cggtaggcga cgaggttcgc
     2761 cgccgagcgg acgccatggg cctatccatc agcgagtaca tcgcgcgcac tctcgcgctc
     2821 gacgtcgggc tccccgacct cgctccacaa gtgcgtctcg acgaggagtt gcctatgacc
     2881 ggctgacccc cgggcatgac gaaggctccc gcgcgaacgg gagcccttgc gtctaggtga
     2941 gaggaccgag cgcgaactcg gtacctctcc gatctgtagc cggttccctt gcccagggag
     3001 gaggcggcgt agccgcccgg cggttttctc atgctcagcg atggaggtcg ctttgtgctg
     3061 cccacgatag cacccgcatg cccggagtcc agtggttcga cgatccggga cgtgtcgcgc
     3121 gtcgccaacg agtcggacgc ggccggcact cccctgccgc gcgcgcgccg gtactaccgg
     3181 ccctgcacgg gcccgcgtga cgtgcgcagc acgatcgcac gctcatgccg cggccgcgcc
     3241 tggcggcccg caaacggccc tacgctgccc gccacggtgc cggtgtggtc cagtgccgcc
     3301 cactggctgg aaacgctcgc agcggccctc
//
LOCUS       DAOSOC010000230         3305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_28992_length_3305_cov_7.975692, whole genome shotgun sequence.
ACCESSION   DAOSOC010000230 DAOSOC010000000
VERSION     DAOSOC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28992_length_3305_cov_7.975692"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            636..2069
                     /locus_tag="PL014_12260"
     CDS             636..2069
                     /locus_tag="PL014_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOT57909.1"
                     /translation="MPPYILEALASSDDPHVAAHAQATLDVDAELREARGIELRPSTA
                     APRRKKRPPRPARGTADAPQRAIHDAERGTTLPGTLVRSEGEADTGDREVREAYDGLG
                     ATWDLWQDTYGRNSLDGRGLPLIATVHFSRDYDNAFWDGTQMVFGDGDGVVFLPFTRS
                     LDVIGHELAHGVTQFTSGLNYQDQSGALNESVSDVFGVLVKQRLLGQSADQADWLIGA
                     DLLGPDVKGVAIRSMAAPGTAYDDPRLGRDPQPGHMRDYVDTQDDNGGVHINSGIPNK
                     AFHVVATTLGGNAWEAAGQVWFDTITGDISADCDFETFAALTVKAATARHGAGSEVVA
                     AVRAGWVAVGLADAVAPPDADPSPSGGGQGSPATPSSPAEPAPKKPTSGTQVNVRRTG
                     GFAGRTVERTVTLGELPKADAKAWRSLLADDHLPALAGRASGGSRMPDSFCYGVRCDA
                     PPIDVELPEPHLSDEVRTLLERTLAAD"
     gene            2128..2616
                     /locus_tag="PL014_12265"
     CDS             2128..2616
                     /locus_tag="PL014_12265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT57910.1"
                     /translation="MSEPDPRTLRRTDFDAVRPVPTRWSDNDMFGHLNNAVYLELFDS
                     VLNAWMQEETGIDEMVAPTLGVVAETSCRYYREVAFPTTLDVGVRAKRIGTTSVVFEF
                     GVFSPGVDEIAAHGLWAQVYVDRETRRPVPIPDAVRTHLEAAVATRAARAATPDTAGS
                     PR"
     gene            2658..3275
                     /locus_tag="PL014_12270"
     CDS             2658..3275
                     /locus_tag="PL014_12270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT57911.1"
                     /translation="MRAWRGPRDDVDGSWQRVAPWHPRAHAVVAVTGRAVITAPDDIS
                     DADLEALGVDGFGQAHDPRVMTRLAGPTGWVDVLDAVLLASGTGSPDPRVVPRPDLID
                     HPRVRHAVPIRSDVRVYGFEGDDESVLTLGVGLGGLAEMSLQVGPNRRGQGLGTVLAA
                     AARGLVPFDEPVVACVSPANVPSLRALLSAGFDPVGSVQVYLPDR"
BASE COUNT          445 a         1308 c         1120 g          432 t
ORIGIN      
        1 cccagcagcg aatggccgcc gcgctgcgcc gctgaggcgc cgtgtacctg cagtgaccta
       61 ttggtcgatc tgggtacggg ccggtcggtg actgagcgga cggcgggtcg gtgattgcgg
      121 ggacggccgg gcggtgaccg cgcgcacggg cggggtgctg acgttcctct ccacggaacg
      181 tgggtggttc caccccccgc accccgcgac tcgggcgtgg ggcgcgaggg tggcaacttc
      241 tcgcgggagt tcttcaccga gcccaaggcc gtcgtcatgc agatcgaccg cggctgaccc
      301 agcccgtacc cacatcgacc aataggtcag tcggggtacg cccccggagt cacggcccgt
      361 acccagatcg accaataggt caccggaggt acgcgccccg accgggccgg cgtacctgga
      421 gtgacctatt ggacgatttc gggacgctcc ttcgggtcgc gggtgtcggt ggctcggggc
      481 aggctggccg tatgccgtcc gctcagcacc cgtcgccgtc ccacccgccg ctgtccgcgc
      541 cccctctggc atcgcctccg accgaccgcc cgttctccgc acccgctcag gcgtcggcac
      601 ggccgcagcc gccgcagccg ccgctgtgca cgatcgtgcc gccgtacatc ctcgaggcgc
      661 tggcctcctc ggacgacccg cacgtcgccg cgcacgccca ggccaccctc gacgtcgacg
      721 ccgagctgcg cgaggcacgc ggcatcgagc tgcgcccctc cactgcggca cccaggcgca
      781 agaagcgacc gccgcgcccg gcacgcggca ccgccgacgc cccgcagcgg gccatccacg
      841 acgccgagcg gggcaccacc ctgcccggca ccctcgtgcg cagcgagggc gaggccgaca
      901 ccggtgaccg cgaggtccgc gaggcctacg acggtctcgg cgccacctgg gacctctggc
      961 aggacaccta cggccgcaac tccctcgacg gccgcggcct gccactcatc gccacggtgc
     1021 acttcagccg cgactacgac aacgccttct gggacggcac ccagatggtc ttcggcgacg
     1081 gtgacggcgt ggtcttcctg cccttcaccc gcagcctcga cgtcatcggc cacgagctcg
     1141 cgcacggcgt cacccagttc acctcgggcc tgaactacca ggaccagtcc ggtgccctca
     1201 acgagtcggt ctccgacgtc ttcggggtgc tcgtcaagca gcgcctcctc ggtcagagcg
     1261 ccgaccaggc cgactggctc atcggcgccg acctgctcgg ccccgacgtc aagggtgtgg
     1321 cgatccgctc gatggccgcc cccggcaccg cctacgacga cccgcgcctg ggacgcgacc
     1381 cgcagccggg ccacatgcgc gactacgtcg acacccagga cgacaacggc ggcgtccaca
     1441 tcaactccgg catcccgaac aaggccttcc acgtcgtcgc gaccacgctc ggcggcaacg
     1501 cctgggaggc cgccggccag gtctggttcg acaccatcac cggcgacatc agcgccgact
     1561 gcgacttcga gaccttcgcc gcgctcacgg tcaaggccgc cacagcccgc cacggggctg
     1621 ggtccgaggt cgtcgccgcc gtgcgcgcgg gctgggtcgc ggtcggtctc gcggatgccg
     1681 tcgccccgcc tgacgcggac ccctcaccgt ccgggggcgg ccagggctcc cccgccacac
     1741 cgtccagccc ggcggagccc gcgccgaaga agccgacctc gggcacgcag gtcaacgtcc
     1801 gccgcaccgg aggattcgcc gggcgcaccg tggagcgcac ggtcaccctc ggtgagctgc
     1861 cgaaggctga cgccaaggcc tggcggtccc tcctggccga tgaccacctg ccggcgctcg
     1921 cgggccgggc ctccggcggc tcccggatgc ccgacagctt ctgctacggc gtgcggtgcg
     1981 acgccccgcc gatcgacgtc gagctgcccg agccgcacct cagcgacgag gtgcgcaccc
     2041 tgctcgagcg caccctggcc gccgactgac gccagcgact gacgcggccg actgaccacc
     2101 ccgaccaccc ggcatacgat cggcgccatg agcgagcccg acccgcgcac cctgcgccgc
     2161 accgacttcg acgccgtgcg ccccgtgccc acccgctggt cggacaacga catgttcggc
     2221 cacctcaaca atgcggtcta cctcgagctg ttcgacagcg tcctcaacgc gtggatgcag
     2281 gaggagaccg gcatcgacga gatggtcgcg cccaccctcg gggtcgtcgc cgagacgagc
     2341 tgccgctact accgcgaggt cgccttcccg acgacgctcg acgtcggggt gcgcgccaag
     2401 cggatcggca ccaccagcgt cgtcttcgag ttcggggtct tctcccccgg ggtcgacgag
     2461 atcgccgcgc acggcctgtg ggcgcaggtc tacgtcgacc gcgagacgcg tcgcccggtt
     2521 cccatcccgg atgccgtccg cacccacctc gaggccgccg tcgccacccg cgccgcccgt
     2581 gctgccaccc ccgacaccgc cgggagcccg cgctgagcac ggacggtgcg ccccggcatc
     2641 cggtcgccga cctgctcgtg cgcgcctggc ggggcccacg cgacgacgtc gacgggtcgt
     2701 ggcagcgcgt cgcgccgtgg cacccccggg cgcacgccgt cgtcgcggtg accgggcgcg
     2761 ccgtgatcac cgcgcccgac gacatcagcg atgccgacct cgaggcgctc ggggtcgacg
     2821 gcttcggcca ggcgcacgac ccgcgcgtga tgacccgcct cgcaggcccg accgggtggg
     2881 tcgacgtgtt ggatgccgtg ctgctcgcct cgggcaccgg gtcgccggac ccgcgcgtcg
     2941 tgccgcgccc ggacctcatc gaccacccgc gggtgcgcca cgcggtgccg atccgcagcg
     3001 acgtgcgggt gtacggcttc gagggtgacg acgagtcggt gctgaccctc ggggtcgggc
     3061 tcggcggcct cgcggaaatg agtcttcagg tggggccgaa ccgccgcggt cagggcctgg
     3121 gcacggtgct ggccgctgcc gccaggggcc tggtgccgtt cgacgagccg gtcgtggcgt
     3181 gcgtgtcacc cgcgaacgtg ccgagcctgc gggcgttgct gagcgccggg ttcgaccccg
     3241 tcggctccgt gcaggtctac ctccccgacc gctgaccgag gccttcgggc cctggcacct
     3301 cgggg
//
LOCUS       DAOSOC010000231         3248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_29661_length_3248_cov_6.940182, whole genome shotgun sequence.
ACCESSION   DAOSOC010000231 DAOSOC010000000
VERSION     DAOSOC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29661_length_3248_cov_6.940182"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1556)
                     /locus_tag="PL014_12275"
     CDS             complement(153..1556)
                     /locus_tag="PL014_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT57912.1"
                     /translation="MTLRRRLVIAVSALTMVLVVAGLGVLLVQRAFLVGRLDAQLAAL
                     AQSPRAILLASARADAGAPAAGLSDIYVGRMAADGTLTTILAPTSDPGLVPALVPGER
                     VTDPQARPTASGEASRVRVVTAPLPNGRAQAVLAVPTTAADAATRRLALTLGLVGLAV
                     AGVVAMLLSWVDRLGLRPIAAMTEAADAIRAGDTARRVPPGPPGTEAARLGDALNAMI
                     DSNAATQERMRRFIADASHELRTPLTTLQGYAALHTGRAGTTATPDAVAPVEGVTSPE
                     VASPEVADAMRRIGEEAARMRRLVDGLLDLARLDDLEALGGLAREPVDLGVLVRDVAA
                     DLRMVAPDRVVTVQAPQSLIVSGDRDRLTQGLVGLTSNAVRHTPAGTAVWLRVVPVPG
                     AVRVDVADAGPGIPPEHVPHVLGRFHRVEAGRSSSRGGSGLGLAIVDAIARAHGGSVS
                     VVSEVGRGSTFTLNLPA"
     gene            complement(1556..2236)
                     /locus_tag="PL014_12280"
     CDS             complement(1556..2236)
                     /locus_tag="PL014_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT57913.1"
                     /translation="MTPRVLVVDDEENIAYLVASALRLAGLEVATAATGEEALAACTP
                     ASPDLVVLDVMLPDLDGFEVLRRLRAKGVTAPVLFLTARTDTADRVRGLTAGGDDYIV
                     KPFALEELVARVRLALRRRGTTPVAPGRHQVHDLVLDEDQHRVWRGGAEVHLTATEFT
                     LLRCLLVNAGRVVTRAQILDHVWQYDFAGESAIIESFISTLRKKVDATTPRLIHTVRG
                     VGYTIREP"
     gene            2518..3060
                     /locus_tag="PL014_12285"
     CDS             2518..3060
                     /locus_tag="PL014_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57914.1"
                     /translation="MSALRTTLVAVGLGAAGLTTTALVATSATAAPAPVAAVEQLAAA
                     NSGTAADAASAGTASADAARTKARGWWAGLTDDQRTCLQDEDITRPVGRLADAERTAL
                     RAKVEAAAKTCDVTLPFAGPRAFWDGLSTEQRDCIRDAGVNRPWGQLTKEQRQAVRAD
                     LRAAAEKCGVALPARSAGRE"
BASE COUNT          455 a         1163 c         1222 g          408 t
ORIGIN      
        1 gtcggcgatg cgagcgaccc gcaggactcg agccaccggc cacctcccac acgattcatc
       61 ccgggcgact ggtcccggct cctgaccgcc ccaccgtatg tgttccggct gacacccgag
      121 gaccttcatc cacaggcggt cggccgggaa ggtcaagcgg gcaggttcag cgtgaaggtg
      181 gacccgcggc caacctcgga cacgaccgac acggacccgc cgtgggcccg ggcgatcgcg
      241 tcgacgatcg ccagccccag cccgctgcca ccacgtgagg acgatcgccc ggcctcgacc
      301 cggtggaagc gcccgagcac gtgcggcacg tgctcgggcg ggatgccggg ccccgcgtcc
      361 gcgacgtcga cccgcaccgc gcccggcacc ggcacgacgc gcagccacac cgcagtcccc
      421 gcgggcgtgt ggcgcacggc gttcgaggtg aggccgacga gcccctgggt cagccggtcg
      481 cggtctccgc tgacgatcag cgactgtggg gcctgcacgg tgacgacccg gtcgggtgcc
      541 accatgcgca ggtcggccgc cacgtcgcgg acgagcaccc ccaggtcgac gggctcgcga
      601 gccagacctc ccagggcctc caggtcgtcg agccgggcca ggtcgagcag cccgtcgacg
      661 agccgacgca tgcgggcggc ctcctccccg atacgacgca tcgcgtcggc cacctcgggg
      721 gaggccacct cgggggaggt caccccctca acgggcgcca cggcatccgg cgtggctgtg
      781 gtgcccgctc ggccggtgtg cagcgctgcg tacccctgca gcgtggtgag cggggtgcgc
      841 agctcgtggg acgcgtcggc gatgaagcgg cgcatccgtt cctgagtggc ggcgttgctg
      901 tcgatcatcg cgttgagggc gtcgccgagc cgcgcggcct cagtgccggg cggccccggc
      961 ggcacccggc gcgcggtgtc gccagcccgg atcgcgtcgg cggcctcggt catcgcggcg
     1021 atgggccgaa gcccgaggcg gtcgacccac gacagcagca tcgcgacgac tcccgccacg
     1081 gcgagcccga cgaggccgag cgtgagcgcg aggcgacggg tcgcggcatc cgcggcggtg
     1141 gtcggcacgg cgagcaccgc ctgggcgcgc ccgttgggca ggggggccgt caccacgcga
     1201 acgcgcgagg cctcgccgga tgccgtgggg cgcgcctgtg ggtccgtgac cctctcgccg
     1261 gggaccagtg cggggacgag gcccgggtcg ctcgttgggg cgaggatcgt cgtgagggtg
     1321 ccgtcggccg ccatccgccc gacgtagatg tccgagagcc cggcggctgg tgcgcccgca
     1381 tccgcccggg ccgacgcgag caggatcgct cgcgggctct gggccagggc ggcgagctgg
     1441 gcgtcgaggc ggccgacgag gaaggcccgt tgcacgagca gtacaccgag gccggcgacg
     1501 acgagcacca tggtcagggc gctgaccgcg atgacgagcc ggcggcgcag cgtcatcacg
     1561 gctcgcggat cgtgtagccg acgccgcgca cggtgtggat gagtctcggg gtggtggcat
     1621 cgaccttctt gcgcagcgtc gagatgaacg actcgatgat cgcgctctcg ccggcgaagt
     1681 cgtactgcca gacgtggtcg aggatctgcg ccctggtgac gacgcgcccg gcgttgacga
     1741 gcaggcagcg caggagggtg aactccgtgg cggtgaggtg cacctcggcg ccgccacgcc
     1801 agacgcggtg ctggtcctcg tcgagcacca ggtcgtgcac ctggtgacgg ccgggcgcga
     1861 ccggggtggt tcctcggcgg cgcagggcca ggcgcacgcg ggcgacgagc tcttcgaggg
     1921 cgaacggctt gacgatgtag tcgtcaccgc cggcggtcag gccgcgcacc cggtcggcgg
     1981 tgtcggtgcg cgcggtgagg aagagcactg gtgccgtcac acccttggcc cgcaggcggc
     2041 gcagcacctc gaagccgtcg aggtcgggga gcatgacgtc gagcacgacg aggtcggggg
     2101 atgccggggt gcaggccgcc agcgcctcct cgccggttgc tgcggtcgcc acctcgagcc
     2161 cggcgagccg cagggccgag gccacgaggt aggcgatgtt ctcctcgtcg tcgacgacga
     2221 gcacccgagg cgtcatgggc cccacgctcc cacctcggac gggtcgcagt ctggaggatt
     2281 gctcgagact tcctgtggat cgctgcgcgt gcggtcccgg gcacgcgtca cctcccggaa
     2341 cgtgggtgaa gatcgtcggc agggagccac tcttcaccca cattcgacgg ggggcgacgg
     2401 cgggtcctca gatgatcgcg aggtttcctt gcagcttcgc gcagtcttcg ggacgaccgt
     2461 cgtgacctgt caccagcccg ggtcggctcg ttggacccca tcgacaagga gcacctcatg
     2521 tccgcactgc gcaccaccct cgtcgccgtc ggcctcggcg ccgcgggcct gacgaccacg
     2581 gccctcgtcg ccacgagcgc gactgccgcc ccggctccgg tcgccgcggt cgaacagctc
     2641 gcggcggcca actcggggac cgccgcagac gcggcatccg caggcacggc atccgcagac
     2701 gcggcccgca cgaaggctcg tggatggtgg gccggtctca ccgatgacca gcgcacctgc
     2761 ctccaggatg aggacatcac ccgcccggtc ggccgcctcg ccgacgccga gcgcacggcc
     2821 ttgcgcgcga aggtcgaggc cgccgcgaag acgtgcgacg tcacgctgcc cttcgccggg
     2881 ccccgggcgt tctgggacgg cctcagcacc gagcagcgcg actgcatcag ggatgccggc
     2941 gtcaaccgcc cgtggggaca gctcaccaag gagcagcgtc aggccgtgcg cgccgacctc
     3001 agggcggccg ctgagaagtg cggggtcgcc ctgccggcac gctcagccgg tcgagagtag
     3061 gcgaatcgcc gtgggcgggt ccagcgcagc ggcgaggtgc ctacccgcga ccggtggacg
     3121 tggtccccga acgcgcggcc aacgccgcgc tccgttcgcg gatgacgtcg tcacatgcgt
     3181 cgaggaagcg agccatgctg gcgccggggg tggtgtcacc gaagtaccgc cgcaccaggg
     3241 ccgcgtgg
//
LOCUS       DAOSOC010000232         3243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_29714_length_3243_cov_8.837202, whole genome shotgun sequence.
ACCESSION   DAOSOC010000232 DAOSOC010000000
VERSION     DAOSOC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29714_length_3243_cov_8.837202"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /gene="lexA"
                     /locus_tag="PL014_12290"
     CDS             complement(<1..701)
                     /gene="lexA"
                     /locus_tag="PL014_12290"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOT57915.1"
                     /translation="MARPRNADIHEMPDRGDGGALTHRQRKVLEVIRNSIDRRGYPPS
                     MREIGDAVGLTSPSSVAHQLSALERKGYLRRDPNRPRAIEVISPDTASDEPGYRGVPD
                     HDDEDVTGINDQRPEATYVPVLGQIAAGVPITADEVVEDVFPLPRQIVGEGSLFLLRV
                     VGDSMIDAAICDGDWVVVRQQPNAENGDIVAAMLDNEATVKTFKRRDGKVWLMPHNPE
                     FSPIDGDHATILGKVT"
     gene            950..1333
                     /locus_tag="PL014_12295"
     CDS             950..1333
                     /locus_tag="PL014_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT57916.1"
                     /translation="MSALAWDHTELGYAGSTPKRPHLVLVQGGARTTADAGGVRLTRR
                     GRLALFLLVLAVVAVTSLTGLRGAGASEPLRAVTVAPGETLSQIAVAELPTLSMDQAI
                     VAIQIANNLSTAQVSAGQQLVIPSS"
     gene            1664..2305
                     /locus_tag="PL014_12300"
     CDS             1664..2305
                     /locus_tag="PL014_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57917.1"
                     /translation="MNRLLKPFVPLVGAAALIAAPVAFAAPAFAAVIEPDDSIPFDPE
                     LLDPTVWEDILAEEEDVDAWCESFGDISTPVDIPEITDAFQLPEDAEPADFGYYLAVL
                     AGFPEEGTEEDPDLELYWEPSVGDEIEHAAGQNLEVILCVAEYEDEVSPSPSPTVSTK
                     PSVKPTVTGPVVETDRVGDSASVPGLGLAAAGAALVAGAGALVLSRRRQGSHR"
     gene            2305..2967
                     /locus_tag="PL014_12305"
     CDS             2305..2967
                     /locus_tag="PL014_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HOT57918.1"
                     /translation="MTSRPLRAVGVALIVLAVALLTWVMQRDGDAVPVAATPAATTPA
                     PSITPSTPSPTPTPTPTPTPTASTSKAPSTEGLRIRIPAIGLDRAMRGEGLSSNGTID
                     PAPGTVMWFKGYERVRPGKVGTAVIAAHVATGKRPDVFADLADVDVGDTIEVVEDGKS
                     VKYRVTRAGAIDKKKVTTDQTVWGANTSRTRLAIITCDDAFGLRGDGHRVANFVVIAD
                     KV"
     gene            complement(2984..>3243)
                     /locus_tag="PL014_12310"
     CDS             complement(2984..>3243)
                     /locus_tag="PL014_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57919.1"
                     /translation="TYRDTGFPRRDPSASRARLVSEAGGGIVVVGDEDVGVWKRVVTA
                     ASVRVAVSGDTPLDTSDQDAIGDAAAAFAAFLERPLDLTFG"
BASE COUNT          470 a         1140 c         1115 g          518 t
ORIGIN      
        1 gtgaccttgc ccaggatcgt cgcgtggtct ccgtcgatgg gtgagaactc cgggttgtgc
       61 ggcatcagcc agaccttgcc gtcgcgccgc ttgaacgtct tgacggtggc ctcgttgtcg
      121 agcatcgcag cgacgatgtc cccgttctcg gcgttgggct gctggcgcac gacgacccag
      181 tcgccgtcgc agatcgcggc gtcgatcatc gagtcgccga cgacccgcag gaggaacagc
      241 gacccctcgc cgacgatctg gcgggggagg gggaagacgt cctcgacgac ctcgtcggcg
      301 gtgatcggca cgccggcggc gatctggccg agaaccggaa cgtaggtggc ctccgggcgc
      361 tggtcgttga tgccggtgac gtcctcgtcg tcgtggtcgg gcacgccgcg gtagccgggc
      421 tcgtcgctcg cggtgtcggg ggagatgacc tcgatcgccc gggggcggtt cgggtcgcgg
      481 cgcaggtagc ccttgcgctc cagcgccgac agctggtggg cgaccgagct gggggaggtc
      541 aggccgacgg cgtcgccgat ctcgcgcatg ctcggcgggt agccgcgccg gtcgatggag
      601 ttgcggatga cctcgaggac cttgcgctgg cggtgggtga gcgccccgcc gtcgccgcgg
      661 tcgggcatct cgtggatgtc ggcgttcctc ggacgtgcca tgtcgacccc ttcggtcacg
      721 gtcatcggcg ctggtgtcag ttccagtctt ttcgcaggtc aggccgactg tcggtgctcg
      781 tcggcaggat ctcctgcatg acgacaccct acgaggctcg tacagagaat tcaaacattt
      841 cttcgaggtg tgtcttgagt ggctcgaaca ttcgtgctac aactcgtaca gacgttctat
      901 cgaacaccgg ttcgacggtg gccccggcca ccgagatgga ggagacatca tgagtgcact
      961 cgcgtgggac cacaccgagc tcgggtacgc cggctcgacc ccgaagcgtc cgcacctcgt
     1021 cctcgtgcag ggtggtgcgc gaaccacggc ggatgccggt ggcgttcgcc tcacgcggcg
     1081 cggtcgcctg gcgctgttcc tcctcgtgct cgcggtcgtg gccgtgacca gcctcaccgg
     1141 gctgcgcggg gcaggggcga gcgagccgct gcgggcggtg acggtcgctc ccggcgagac
     1201 gctgtcgcag atcgcggtgg ccgagctgcc gacgttgtcg atggatcagg ccatcgtcgc
     1261 gatccagatt gccaacaacc tcagcaccgc gcaggtcagc gccggtcagc agctggtgat
     1321 cccgagctcc tgaccctcat cccaccgggt gcccgtgagg ccgccggtgc tccagacctg
     1381 agtccctcga cccggctcag agacgccgaa gggcgtcgcc cccgtgcagg ggtgcggcgc
     1441 ccttcgtgct gctcggctct cacgcagtgg cgttcgaggt gctggacctg gtcgatgtgg
     1501 gcggccggcc cgcccgtggt catcagccga atggggtgtg cgggtcgtgc gctgcgacgc
     1561 tatcgttccg ggtgagcggg ggatcaacgg ctctgtccag tccctgtgtc attcatcagt
     1621 cactggcggg tctgtgctgg cccgttgtcg aaaggtctgc cccatgaacc gcctcctcaa
     1681 gcccttcgtg ccgctcgtcg gggccgctgc cctcatcgcg gcacccgtcg ccttcgcggc
     1741 tccggcgttc gccgccgtga tcgagcccga cgactccatc ccgttcgacc ccgagctgct
     1801 tgacccgacg gtgtgggagg acatcctcgc cgaggaggag gacgtcgacg cgtggtgtga
     1861 gtccttcggc gacatcagca ccccggtcga catccccgag atcaccgacg ccttccagct
     1921 tcctgaggat gccgagcccg cagacttcgg ctactacctc gcggttctcg cgggcttccc
     1981 cgaagagggc acggaggagg accctgacct cgagctctac tgggagccct cggtcggtga
     2041 tgagatcgag cacgccgccg gtcagaacct tgaggtcatc ctctgcgtcg ccgagtacga
     2101 ggacgaggtc agcccgagcc cgtcgccgac cgtcagcacg aagccctcgg tgaagcccac
     2161 cgtcaccggc cccgtggtcg agaccgaccg cgtcggtgac tccgcctccg tgccgggtct
     2221 tgggctggcc gccgccggcg ccgcgctcgt cgccggagca ggcgccctcg tcctcagccg
     2281 ccgccgtcag ggcagccacc gctgatgact tctcgcccac tgcgtgcggt gggcgtggcc
     2341 ctgatcgtcc tcgccgtcgc gctgctcacc tgggtgatgc agcgcgacgg cgatgccgtt
     2401 cccgtcgcgg ccaccccagc cgcgacgacc ccggcgccat cgatcacccc gagcactccc
     2461 agccccacgc caacgcccac gccgacacca accccgacgg catccacgag caaggcgccc
     2521 tcgaccgagg gcttgcgcat ccggatcccg gccatcgggc tggaccgcgc catgcgcggg
     2581 gaggggctga gctcgaacgg cacgatcgac ccagccccgg gcaccgtgat gtggttcaag
     2641 ggctacgagc gggttcgccc cggcaaggtc ggcacggcgg tcatcgccgc gcacgtcgcc
     2701 accgggaagc ggcccgacgt ctttgccgac ctggccgacg tcgacgtcgg cgacaccatc
     2761 gaggtcgtcg aggacggcaa gtcggtgaag taccgcgtga cccgggcggg ggccatcgac
     2821 aagaagaagg tcacgaccga ccagacggtc tggggtgcga acacctcgcg gaccaggctg
     2881 gcgatcatca cctgcgacga cgcgttcgga ctgcgaggtg acggccaccg ggtcgccaac
     2941 ttcgtcgtca tcgctgacaa ggtctgacgc cgcggcgccg acgctagccg aaggtcaggt
     3001 cgagagggcg ctccaggaaa gcagcgaagg ccgcggccgc gtcaccgatc gcgtcctgat
     3061 cgctggtatc gagaggggtg tcgcccgaaa cggccacccg aaccgacgcg gccgtcacca
     3121 cgcgcttcca caccccgacg tcctcatcac ccacgacgac gatgccccca cctgcctccg
     3181 acaccagccg ggcccgcgac gccgacgggt cgcgccgcgg gaagccggtg tcgcggtagg
     3241 tca
//
LOCUS       DAOSOC010000233         3205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_30114_length_3205_cov_6.258095, whole genome shotgun sequence.
ACCESSION   DAOSOC010000233 DAOSOC010000000
VERSION     DAOSOC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30114_length_3205_cov_6.258095"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PL014_12315"
     CDS             complement(<1..1051)
                     /locus_tag="PL014_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT57920.1"
                     /translation="MTIIDEPALETPDHGTPPVWGEPSAHVTIDGITVMVPPGTSVMR
                     AAKIAGIDVPKLCATDSLKAFGSCRLCLVEVEGMRGTPASCTTPCTDGMVVSTRTEQV
                     QQLRRGVMELYLSDHPTDCAGCERGNCEMQGLAHDVGLAEVRYGLGGASHLDEAVDQS
                     NPYFAYDPKACIVCSRCVRACSETQGTFALTVQGRGFDSRITAGGTDFLSSECVSCGA
                     CVQACPTDALTEKSVIQLGMPSRSVITTCAYCGVGCSFKAEVQGSGDATRVVRMVPWK
                     DGGANEGHSCVKGRFAYGYASHRDRQLSPMVRDSIDDEWQVVSWEEAITRVAEGFLAL
                     REKHGVGSIGGISSSR"
     gene            complement(1048..2589)
                     /locus_tag="PL014_12320"
     CDS             complement(1048..2589)
                     /locus_tag="PL014_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT57921.1"
                     /translation="MSTAWVSCDSAAVAAGADAVAEAIAAAGITVRRHGSRGMLWLEP
                     LVELETEGGRVGYGNITEAEVGPLLDGALDDTDRCIGAVDDHPWMARQHRVSFARVGV
                     IEPTDLAAYREHGGLAGLLRAIELTPEAVVAEVTASGLRGRGGAGFPAGIKWETVRTA
                     SADVKFVCCNADEGDSGTFADRMLIEGDPFTLIEGMAIAALAVGATEGFVYLRSEYPL
                     AITTLRRAIEICYAHGILGESVLGSGRRFDLDVRVGAGAYICGEETSMLESLEGKRGE
                     VRAKPPIPALEGLFGRPTIVNNVLTLAAVPMVLADGGEAYAALGTGRSRGTQVLQLAG
                     NVARGGIVEADFGITLDTLVNDFGGGTASGRPVKAVQVGGPLGAYLPPSQFALPMDYE
                     AFAEAGAMVGHGGIVVWDDTLDAAAMARFAMEFCAKESCGKCTPCRVGSIRGVEVIDR
                     VIAAPDDGVRRTQLTLLEDLCTTMTKGSLCAMGGLTPMPVRSAIEHFPQDFGIPDDTT
                     QGARS"
     gene            complement(2586..3071)
                     /locus_tag="PL014_12325"
     CDS             complement(2586..3071)
                     /locus_tag="PL014_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT57922.1"
                     /translation="MRQRGPAPAAAPTPADPARAASVRALAEQFTDLRGPLLPILHAV
                     VAAHGHVHDDDIPVIADVLNLSRADVHGVVTFYHDFRRTPPVAHRVSLCRGEACQARG
                     AQTSYTAATERWSASDDVEVAEVFCLGLCAQGPSGQIDGALHTALTPERIEALTTGWQ
                     A"
BASE COUNT          453 a         1111 c         1203 g          438 t
ORIGIN      
        1 accgcgacga ggagatgccg ccgatcgaac ccacgccgtg cttctcgcgc agggcgagga
       61 agccctcggc gacgcgggtg atggcctcct cccacgacac gacctgccac tcgtcgtcga
      121 tcgagtcacg caccatcggg gacagctgcc ggtcgcggtg cgaggcgtag ccgtaggcga
      181 accggccctt gacgcagctg tgcccctcgt tggcaccgcc gtccttccac ggcaccatcc
      241 gcacgacgcg cgtcgcgtcg ccgctgccct gcacctcggc cttgaaggaa cagccgaccc
      301 cgcagtaggc gcacgtcgtg atcacgctgc gcgacggcat ccccagctgg atcaccgact
      361 tctcggtgag ggcgtcggtc gggcacgcct ggacgcaggc gccgcaggag acgcactccg
      421 aggacaggaa gtcggtgcca ccggcggtga tccgcgagtc gaagccgcgc ccctgcacgg
      481 tgagggcgaa ggtgccctgg gtctcggagc aagcgcgcac acagcgcgag cagacgatgc
      541 aggccttcgg gtcgtaggcg aagtacgggt tggactgatc gaccgcctcg tcgaggtgcg
      601 aggcgccgcc gaggccgtag cgcacctcgg ccaggccgac gtcgtgcgcc agcccctgca
      661 tctcgcagtt gccgcgctcg cagccggcgc agtcggtggg atggtcggac aggtagagct
      721 ccatgacacc gcgacgcagc tgctggacct gctcggtgcg ggtcgacacc accatgccgt
      781 cggtgcacgg ggtggtgcag gacgcgggcg tgccacgcat gccctcgacc tcgacgaggc
      841 acaggcggca ggagccgaag gccttcagcg agtcggtcgc gcacagcttc ggcacgtcga
      901 tgccggcgat cttcgcggcg cgcatcaccg acgttcccgg cggcaccatg accgtgatgc
      961 cgtcgatggt gacgtgcgcg ctcggttccc cccagaccgg tggcgtgccg tggtcgggtg
     1021 tctcgagggc gggctcgtcg atgatcgtca tgaacgcgct ccttgcgtgg tgtcgtccgg
     1081 gatgccgaag tcctgcggga agtgctcgat ggcgctgcgc accggcatgg gggtgaggcc
     1141 acccatggcg cacagcgagc ccttggtcat cgtcgtgcag aggtcctcga ggagggtgag
     1201 ctgggtgcgg cgcacgccgt catcgggtgc ggcgatgacg cggtcgatga cctcgacccc
     1261 gcgaatcgac ccgacgcggc agggcgtgca cttgccgcag gactccttgg cgcagaactc
     1321 catggcgaag cgggccatgg ccgcggcgtc gagggtgtcg tcccacacga cgatgccgcc
     1381 gtgcccgacc atcgccccgg cctccgcgaa cgcctcgtag tccatcggca gggcgaactg
     1441 cgacgggggg aggtaggcgc ccagagggcc gccgacctgc accgccttca cgggacgccc
     1501 ggatgccgtg cccccaccga agtcgttgac cagggtgtcg agcgtgatgc cgaagtcggc
     1561 ctcgacgatg ccgccgcgcg cgacgttgcc ggcgagctgg aggacctgcg tgccgcgcga
     1621 gcgtccggtg ccgagggccg cgtacgcctc gcccccgtcg gcgagcacca tcgggacggc
     1681 cgccagggtg agcacgttgt tgacgatcgt gggccgcccg aagagcccct cgagcgccgg
     1741 gatcgggggc ttggcccgca cctcgccgcg cttgccctcg agggactcga gcatcgaggt
     1801 ctcctcgccg cagatgtagg cgccagcgcc cacccgcacg tcgaggtcga agcggcgccc
     1861 gctgccgagc acggactcac cgaggatgcc gtgggcgtag cagatctcga tggcgcggcg
     1921 cagggtggtg atggcgagcg ggtactcgct gcgcaggtag acgaacccct cggtcgcacc
     1981 gacggccagg gcggcgatgg ccatgccctc gatgagggtg aacgggtcgc cctcgatgag
     2041 catgcggtcg gcgaacgtgc cgctgtcgcc ctcgtcggcg ttgcagcaga cgaacttcac
     2101 gtcggcggat gccgtgcgca ccgtctccca cttgatgccg gccgggaacc cggcgccacc
     2161 gcgcccgcgc agcccggagg ccgtgacctc ggcgacgacc gcctcggggg tgagctcgat
     2221 ggcgcgcagc agcccggcga ggccgccgtg ctcccggtag gcggcgaggt cggtcggctc
     2281 gatgaccccg acccgggcga aggacacccg gtgctggcgg gccatccacg ggtggtcgtc
     2341 gacggccccg atgcaccggt cggtgtcgtc cagggccccg tcgagcagcg gccccacctc
     2401 ggcctcggtg atgttgccgt acccgacccg gccgccctcg gtctcgagct cgacgagcgg
     2461 ctcgagccac agcatcccgc gagagccgtg gcgccgcacc gtgatcccgg ctgccgcgat
     2521 cgcctcggcc acggcgtcgg cgccagcggc gacggccgcg ctgtcgcagg agacccacgc
     2581 ggtgctcatg cctgccaccc cgtggtgagg gcctcgatgc gctcgggggt gagggcggtg
     2641 tgcagcgcgc cgtcgatctg cccggacggg ccctgggcgc acaggcccag acagaacacc
     2701 tcggcgacct cgacgtcgtc ggaggcgctc cagcgctcgg tggctgcggt gtaggacgtc
     2761 tgcgccccgc gggcctggca ggcctcgccg cggcacaggc tgacgcggtg ggcgaccggg
     2821 ggagtgcgcc ggaagtcgtg gtagaaggtc acgaccccgt gcacgtcggc ccgcgagagg
     2881 ttgaggacgt cggcgatgac ggggatgtcg tcgtcgtgga cgtggccgtg cgccgcgacg
     2941 accgcgtgga ggatcgggag cagggggccg cgcaggtcgg tgaactgctc ggcgagggct
     3001 cgcacggatg ccgcccgggc ggggtctgcc ggggtagggg ccgcggccgg ggccggtcct
     3061 cgttggcgca cgtcatcgac tcctcaacgg tgagtgtgat ctgggtcgca actgtacgcg
     3121 cggctcgggc ggagtgtggc gtgcaccccg cggaggaggg gtgcacgcca cactcgtggt
     3181 tcgctcagaa cgacgacatg ggcac
//
LOCUS       DAOSOC010000234         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_30443_length_3177_cov_8.057335, whole genome shotgun sequence.
ACCESSION   DAOSOC010000234 DAOSOC010000000
VERSION     DAOSOC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30443_length_3177_cov_8.057335"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..650)
                     /locus_tag="PL014_12330"
     CDS             complement(75..650)
                     /locus_tag="PL014_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOT57923.1"
                     /translation="MTSTTDNSTTQTSTTQKVAFLVATEGIEQVELTGPWQAVEQSGA
                     TPVLISTQEGTVQAYNHLDKGDVFDVDLTVSSAEVADYAALVLPGGVANPDALRTDSD
                     AVAFVRQFVQSGKPVAAICHAAWTLVEADVVRDRTVTSWPSLQTDLRNAGATWQDQEV
                     VVDDNLITSRNPDDIPAFTRALTAALSSVTA"
     gene            783..1082
                     /locus_tag="PL014_12335"
     CDS             783..1082
                     /locus_tag="PL014_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57924.1"
                     /translation="MALHCAATLVLVPVGTVPDDAAGSGAVVVGALAPGADVVEELQA
                     VADLHRGERVVVPLAPGHLTAVHEHLGRAPGLCDDVVVLEIGDDGWALIRPGTDR"
     gene            complement(1167..1862)
                     /gene="nucS"
                     /locus_tag="PL014_12340"
     CDS             complement(1167..1862)
                     /gene="nucS"
                     /locus_tag="PL014_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HOT57925.1"
                     /translation="MRLVIATCSVDYDGRLTAHLPLATRLLLVKADGSVLVHSDGGSY
                     KPLNWMSPPCAMAEVTPDETEAAEGVTAVWVVQHAKSEDRLRVKIHEVHHDSAHELGV
                     DPGLVKDGVEAHLQKLLAEHITTLGEGWTLTRREYMTPIGPVDILCKDGRGGTVAIEI
                     KRRGDIDGVEQLTRYLELMNRDPHLAPVTGVFAAQVIKPQARTLAEDRGIRCVVLDYD
                     ELKGVDNSEHRLF"
     gene            1930..2370
                     /locus_tag="PL014_12345"
     CDS             1930..2370
                     /locus_tag="PL014_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57926.1"
                     /translation="MRAALLLGFLLVLPGAWFIATGEGLRWFAGGERPRLLRPLLARL
                     DPWIERMDRERRRLLGRRPPEPVPPVLITLELRRLEAEIRKVENGDAPHQAARLRAVL
                     AAYDQVLLQLCERLDVPTDVGMPPLPSQDRLAIEAELVAAGHDW"
     gene            2448..>3177
                     /locus_tag="PL014_12350"
     CDS             2448..>3177
                     /locus_tag="PL014_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOT57927.1"
                     /translation="MTRDFSTVGVIGLGTMGAGIVEVFARNGIDVVAVEVDGPAIEHG
                     KAVLGGSTGRALARGKMTQQEHDALHSRVTFTSNLGDLAPCQLVVEAVPEHLDLKKEL
                     LGKVDAVVSADAVLATNTSSLPVTEIAVATKNPKRVVGMHFFNPAPVQQFVEVIRTVI
                     TADDVFEDVKALAERLGKKPVVVGDKAGFIANALLFGYLNHAVSMFESRYATREDLDA
                     AMRFGCGYPMGPLALLDLIGLDTAY"
BASE COUNT          424 a         1082 c         1160 g          511 t
ORIGIN      
        1 ctcgccgccg acgcagccgc gcccgcccac gcctgaggcg tgggcgggcg ccagcggggc
       61 ggctcggcgg tcgctcaggc ggtcacggac gacagggccg cggtcagcgc gcgggtgaac
      121 gccgggatgt cgtcggggtt gcggctggtg atcaggttgt cgtcgacgac gacctcctgg
      181 tcctgccagg tcgcacccgc gttgcgcagg tcggtctgca ggctcggcca cgaggtgacc
      241 gtgcggtcgc gcacgacgtc ggcctcgacg agcgtccagg ccgcgtggca gatcgcggca
      301 accggcttgc cggactgcac gaactggcgc acgaaggcca cggcatcgga gtcggtgcgc
      361 agggcgtccg ggttggcgac gccgccgggc agcaccagcg ccgcgtagtc ggcgacctcg
      421 gcggaggaca cggtgaggtc gacgtcgaag acgtcaccct tgtcgaggtg gttgtaggcc
      481 tgcaccgtgc cttcctgggt cgagatgaga accggcgtgg cgccggactg ctcgacggcc
      541 tgccaggggc cggtcagctc gacctgctcg atgccctcgg tggcgacgag gaaggcgacc
      601 ttctgggtcg tgctcgtctg ggtggtgctg ttgtcggtcg tgctggtcat ggtggtgtcc
      661 tctcggtccg acgtgttgac ggcgccggtt ggcgccggtg ggacaaccgg agccctcccc
      721 acgtcattcc ccgctgagcc gtgtggccca cgggcgtcga cggtgggggt agcgtgccga
      781 gcatggctct gcactgtgct gcgaccctgg tgctcgtgcc ggtcggcacg gtgccggacg
      841 acgcggccgg tagtggtgcc gtggtggtgg gtgcgctcgc gccgggggcc gacgtcgtcg
      901 aggagctgca ggccgtggcc gacctgcacc ggggtgaacg ggtcgtggtg cctctcgccc
      961 ccggccacct gaccgccgtg cacgagcacc ttggcagagc acccgggctc tgcgacgacg
     1021 tggtggtcct ggagatcggc gacgacggct gggcgctgat ccgcccgggg actgaccggt
     1081 agggcggggc gtacctcggg tgacctgttg gtcgattcag gtacgcgggg cggccgctca
     1141 ctgcggtggg tcggcccggc atccgctcag aagaggcggt gctcgctgtt gtcgacaccc
     1201 ttgagctcgt cgtagtcgag cacgacacag cggatgccgc ggtcctcggc gagcgtgcgc
     1261 gcctgcggct tgatcacctg ggccgcaaag acaccggtca ccggggccag gtgcgggtca
     1321 cggttcatca gctcgaggta gcgggtcagc tgctccaccc cgtcgatgtc gccgcgccgc
     1381 ttgatctcga tggcgaccgt gccgccgcga ccgtccttgc acaggatgtc gaccgggccg
     1441 atgggggtca tgtactcgcg gcgggtgagg gtccagccct cgccgagggt ggtgatgtgc
     1501 tcggcgagca gcttctgcag gtgcgcctcg acgccgtcct tgaccaggcc ggggtcgaca
     1561 ccgagctcgt gggcgctgtc gtggtgcacc tcgtggatct tgaccctcag ccggtcctcc
     1621 gacttcgcgt gctgcacgac ccagacggcc gtgacgccct cggcggcctc ggtctcgtcg
     1681 ggggtgacct cggccatcgc gcacggtggc gacatccagt tcagcggctt gtacgagccc
     1741 ccgtcggagt gcacgagcac cgaaccgtcg gccttgacga gcagcaaccg ggtcgccagc
     1801 ggcaggtggg cggtcagccg cccgtcgtag tcgacgctgc acgtcgcgat caccagtcgc
     1861 acgatcggtc accctaccca ttgcccggtc cgcccttggc agcgcacact tttccttgag
     1921 gtgaggccga tgagggcagc tctgctcctg ggattcctcc tcgtgctgcc cggagcatgg
     1981 ttcatcgcca ccggtgaggg gcttcgctgg ttcgccggtg gggagcgccc gcgactgctg
     2041 cgccccctgc tggcacgact ggacccgtgg atcgagcgga tggaccgcga gcgccgacgg
     2101 ttgctcgggc gtcgaccgcc ggagccggtc ccacccgtgc tcatcaccct cgagctgcga
     2161 cggctcgagg ccgagatccg caaggtcgag aacggggacg caccgcacca ggcagcccgg
     2221 ctgcgggccg tcctcgcggc ctacgaccag gtgctgctcc agctgtgtga gcggttggac
     2281 gtgccgaccg acgtcgggat gccgcccctg ccctcccagg accggctcgc catcgaggcc
     2341 gagctggtcg cggccggtca cgactggtga ctcaccggat cgggggtaag gagcgtcacg
     2401 ctgcgagggg gtcgtgactc cctcgcccct ctgacaggat cgcggacatg acgcgtgact
     2461 tctccactgt tggtgtgatc gggctcggga cgatgggtgc ggggatcgtg gaggtgttcg
     2521 cccgcaacgg gatcgacgtc gtcgcggtcg aggtcgacgg cccggccatc gagcacggca
     2581 aggccgttct cggggggagc accgggcgcg cgctggcccg cggcaagatg acgcagcagg
     2641 agcacgacgc gctgcactcg cgggtcacgt tcacgagcaa cctcggggac ctcgccccct
     2701 gccagctcgt cgtcgaggcc gtgccggagc acttggacct caagaaggaa ctgctcggca
     2761 aggtcgacgc ggtggtctcc gccgacgcgg tcctcgcgac gaacacctcg tccctgccgg
     2821 tcaccgagat cgccgtcgcg acgaagaacc ccaagcgcgt cgtcggcatg cacttcttca
     2881 accccgcccc ggtgcagcag ttcgtcgagg tcatccgcac cgtcatcacc gccgacgacg
     2941 tcttcgagga cgtcaaggcg ctcgcggagc ggctgggcaa gaagccggtc gtcgtcggcg
     3001 acaaggccgg cttcatcgcc aacgcgctgc tcttcggcta cctgaaccac gccgtctcga
     3061 tgttcgagag ccgctacgcc acccgcgagg acctcgacgc cgcgatgcgc ttcggctgcg
     3121 gttacccgat gggcccgctc gcgctgctcg acctcatcgg cctcgacacg gcatacg
//
LOCUS       DAOSOC010000235         3164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_30638_length_3164_cov_8.563847, whole genome shotgun sequence.
ACCESSION   DAOSOC010000235 DAOSOC010000000
VERSION     DAOSOC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30638_length_3164_cov_8.563847"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..827
                     /locus_tag="PL014_12355"
     CDS             114..827
                     /locus_tag="PL014_12355"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOT57928.1"
                     /translation="MSRWHPQAWSITTEAPRPDAELVAELARFPTTQIADGGGPIAVV
                     GPGIGPMVAATEICGPAVTLWTAPGDILYILKSPDLVQPGDVLVIDGGGRLDAAVIGD
                     VIGGRLLANGCAGIVADAAVRDLEGLEALGLPVHARGAHPATGSNVGPGALNVPIQCG
                     GVAVHPGDVVRADRSGVVVVRAGGLAEVVERTRAVGAREDEWQAAFADGAGLDAVFGI
                     DALIAEKAAAAGATGGPEA"
     gene            824..1969
                     /locus_tag="PL014_12360"
     CDS             824..1969
                     /locus_tag="PL014_12360"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOT57929.1"
                     /translation="MTTAAVERTTIGGLPIDVEHTEHPLTAAARAERMREPGFGRVFS
                     EHMLTIRWTRDGGWQDARLTPYGPLVLDPATSALHYGQSVFEGFKVYRGADGSVAAFR
                     PEANARRFQDSSRRLCLPELPVDVFVDAVDLLVAHDHEWVPTAPGHTLYIRPLLFASE
                     PELVVRPSEECLFVLTAFVAADYFPRGLKPVSVWVADHFVRAAPGGTGAAKCAANYAA
                     GLAAQAEAAEHGCDQVVFLDAVERRWIEELGGMNLLFVYGGDVPRIVTPELTGTLLPG
                     ITRDSLLAIARDLGYPVEERRISIDEWEADVRSGAMTEAFACGTAAVLTPVGRVRRGD
                     AEWTIGDGGTGPVTASLRERLLAVQTGAAPDPHGWLRRIEPSGALRP"
     gene            complement(1959..3062)
                     /locus_tag="PL014_12365"
     CDS             complement(1959..3062)
                     /locus_tag="PL014_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57930.1"
                     /translation="MTTEATVVFVTVVALRFLVPLLIPKFPLPAILACLVIDGIDQTI
                     FQTFGFDPPGYQNYDKAMDVYYLAIAFLSSMQNWTHSGAVRISRFLFFYRMIGVMAFE
                     LTGTRALLLIFPNTFEYFFIAYEVIRLRWDPRRFSTRFWILTAAAIWIFVKLPQEYWI
                     HIAQLDFTDTWKEVSWFAPLIITAVLAGLAILWFVVRPRLLAPDWAWRVAADPLPEEI
                     DTAAERDAWVAANVTVWSWWTLEKVVLIGLLSTIYARILPGLETSDLRMFVGIGAYVV
                     VNSAISLLVARRVGSREGLAAAFFARSLTNVGLVLVARLILGGNALDVGATLFFVLLL
                     SLLVTMHDRFAPVGAVRAEHHRADPEPAPAVRA"
BASE COUNT          462 a         1097 c         1178 g          427 t
ORIGIN      
        1 ctgagcgcgt gctgtccgcg gggtggctca acccttgtcg ggcactgcca ccggggcgag
       61 gatgagcccg cccgggcgga taccccgccc gggatcccca tgaggaggtc cgcatgagcc
      121 gttggcaccc ccaggcgtgg tcgatcacca ccgaggcgcc tcgtccagac gccgagctcg
      181 tcgccgagct cgcccgcttt cccacgaccc agatcgccga cggtggcgga cccatcgccg
      241 tggtcggtcc cggcatcggg ccgatggtgg cggcgaccga gatctgcggg cctgcggtca
      301 ccttgtggac ggcaccgggc gacatcctct acatcctgaa gtcacccgac ctcgtgcagc
      361 cgggcgacgt cctcgtcatc gacggcggcg gtcggttgga tgccgcggtc atcggcgacg
      421 tcatcggtgg ccgtctgctg gccaacggct gtgcgggcat cgtcgccgac gccgccgtgc
      481 gtgacctcga gggcctcgag gcgctgggcc tgcccgtcca tgcccgtggc gcccacccgg
      541 ccaccggctc gaacgtcggt cccggggccc tcaacgtgcc gatccagtgc ggcggcgtcg
      601 ccgttcaccc cggggacgtc gtgcgggccg accgcagcgg cgtcgtcgtc gtgcgcgccg
      661 gcgggctggc cgaggtcgtc gagcgcaccc gcgccgtcgg ggcccgcgag gacgagtggc
      721 aggcggcgtt cgcggacggc gccggactcg acgccgtgtt cggcatcgac gccctcatcg
      781 ccgagaaggc ggcagcggcc ggcgccacag gggggccgga agcatgacga ccgcagcggt
      841 ggagcggacc accatcggcg gtctgccgat cgacgtcgag cacacggagc accccctgac
      901 agcggccgcg cgggccgaac gcatgcgcga gcccgggttc gggagggtct tctccgagca
      961 catgctgacg atccgctgga cccgcgacgg cggctggcag gacgctcgtc tcacgcccta
     1021 cgggccactc gtcctggacc cggcgacctc ggcgctgcac tacggccagt cggtgttcga
     1081 ggggttcaag gtctaccgcg gtgccgacgg gtcggtcgcg gccttccggc ccgaggccaa
     1141 cgcccgacgg ttccaggaca gctcgcgccg gctctgcctg cccgagcttc ccgtggacgt
     1201 cttcgtcgac gccgtcgacc tgctcgtcgc ccacgaccac gagtgggtgc cgacggcgcc
     1261 ggggcacacc ctgtacatcc ggccgctgct gttcgccagc gagcccgagc tcgtcgtccg
     1321 gccgtcggag gagtgcctgt tcgtgctcac cgcgttcgtc gcggccgact acttcccgcg
     1381 cgggttgaag ccggtctcgg tgtgggtggc ggaccacttc gtgcgggcgg ctcccggcgg
     1441 caccggcgcg gcgaagtgcg cggcgaacta cgcggccgga ctggccgcgc aggccgaggc
     1501 ggccgagcac gggtgcgacc aggtggtctt cctcgacgcc gtcgagcggc ggtggatcga
     1561 ggagctgggc ggcatgaacc tgctcttcgt ctacggcggg gacgtgccgc gcatcgtcac
     1621 ccccgagctc acgggcaccc tgctgccggg catcacccgc gactccctgc tcgccatcgc
     1681 ccgcgacctc ggctacccgg tggaggagcg gcgcatctcc atcgacgagt gggaggccga
     1741 cgtccggtcc ggggcgatga cggaggcgtt cgcgtgcggc accgcagccg tgctgacgcc
     1801 cgtgggtcgg gtgcgtcgcg gcgacgcgga gtggacgatc ggcgacggag gcaccggccc
     1861 ggtcaccgcc tcgctgcgtg agcggctgct ggccgtgcag accggcgcag cgcccgaccc
     1921 gcacgggtgg ctgcgccgga tcgagccctc cggggcgctc aggccctgac cgcgggtgcc
     1981 ggctccgggt cggcgcggtg gtgttccgca cgcaccgcgc cgaccggtgc gaaccggtcg
     2041 tgcatcgtga cgagcaggga gagcaggagc acgaagaaca gcgtggcccc cacgtccagg
     2101 gcgttgccgc cgaggatgag cctggccacc aggacgagcc cgacgttcgt cagtgaccgg
     2161 gcgaagaatg cggcggcgag gccctcgcgg ctgcccacgc gacgggcgac gagcagcgag
     2221 atcgccgagt tgacgacgac gtaggcgccg atgccgacga acatccgcag gtcggaggtc
     2281 tccaggccgg gcaggatccg ggcgtagatc gtcgacagca ggccgatgag cacgaccttc
     2341 tccagggtcc accacgacca caccgtgacg ttggccgcga cccaggcgtc gcgctcggcg
     2401 gcggtgtcga tctcctccgg cagcgggtcg gcggcgacgc gccaggccca gtccggcgcc
     2461 aggaggcggg gacgcacgac gaaccagagg atggcgagcc cggccagcac ggcggtgatg
     2521 atgagcgggg cgaaccagga cacctccttc caggtgtcgg tgaagtcgag ctgggcgatg
     2581 tggatccagt actcctgcgg cagcttgacg aagatccaga tcgcggcggc ggtgaggatc
     2641 cagaaccgcg tgctgaaccg gcgcgggtcc cagcgcagcc tgatcacctc gtaggcgatg
     2701 aagaagtact cgaacgtgtt ggggaagatg aggagcaggg cccgcgtgcc ggtcagctcg
     2761 aaggccatca cgccgatcat ccggtagaag aacaggaacc gggagatccg cacggccccg
     2821 ctgtgcgtcc agttctgcat cgaggacagg aaggcgatgg cgaggtagta gacgtccatc
     2881 gccttgtcgt agttctggta cccgggcggg tcgaacccga acgtctggaa gatcgtctgg
     2941 tcgataccgt cgatcaccag gcacgcgagg atggccggca gcgggaactt cgggatgagc
     3001 aacggcacga ggaagcgcag cgccaccacg gtgacgaaga cgacggttgc ctcggtggtc
     3061 atgtcgactc ctggtgcgcg gcccgcgggt gagggcgttc gcgtgaacgc tagcggaggg
     3121 cgcggctgcg gcgccccggc atccacgacg ctcacccacg gcgg
//
LOCUS       DAOSOC010000236         3140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_30956_length_3140_cov_9.922853, whole genome shotgun sequence.
ACCESSION   DAOSOC010000236 DAOSOC010000000
VERSION     DAOSOC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30956_length_3140_cov_9.922853"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="PL014_12370"
     CDS             <1..1650
                     /locus_tag="PL014_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016663249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOT57931.1"
                     /translation="FRIAVDLVDEGVITEEEALLRVTGDQLAQLMFPHFDPQGARSLL
                     AKGMSASPGAAVGVAVFDSEAAVRLADAGTPVILVRRETNPDDLHGMVAAAGILTSRG
                     GKTSHAAVVARGMGRTCVCGAETLEVDTRTRQFTTPDGTVVREGDTISIDGSTGEIFA
                     GEVAVRPSPVVRYFEGTMSVEEGGPLVAAVDRLMTVADRHRRMQVRANADNAEDAARA
                     RRFGAQGIGLCRTEHMFLGERRELVERLILAEGPAERQAVFGELLPLQRADFIEIFEA
                     MDGLPVTVRLLDPPLHEFLPDFTELSVKVAVAEATGREHDTDSLLLLAVQRLHEENPM
                     LGLRGVRLGIAIPGLFEMQSQAILEAAAIVAERGGHARPEIMVPLVGAVEELRTIRLE
                     IDEAAHEARVKHAVDLEHLVGTMIELPRAALTAGEIAQAADFFSFGTNDLTQTTWGFS
                     RDDVESSFFSRYMEAGILGSSPFENIDRDGVGRLVRIGTDEGRRTKPGLKVGVCGEHG
                     GDPASVHFFEERRLDYVSCSPFRVPVARLEAARAVLSGPAS"
     gene            complement(1652..2515)
                     /locus_tag="PL014_12375"
     CDS             complement(1652..2515)
                     /locus_tag="PL014_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT57932.1"
                     /translation="MPAATTPQPPSDPGSGPPELERIGDRDLGQWPTRAGQHLVAAIA
                     RVVIVLGRVWRWSLANLALVLTTVIGGAIVLGATVLSAEVYEAVVENDGLAALDEPVL
                     EAAVNSRTPASEQWVTHFTDLGGTVGMPLIAAAIVITLAVRRRSWLPVVLMLIAAAGS
                     VLITVLGKDLTARARPPVIYAVPPLETSPSFPSGHTLNATVVLGLSAYLVMLGRRHLR
                     SRILVASVVVVFVVAMGLSRVWLGHHWLTDVMAGWLVGLAWLAAVITGHRVKVTLDRA
                     ERARPEGRGRG"
     gene            complement(2549..>3140)
                     /locus_tag="PL014_12380"
     CDS             complement(2549..>3140)
                     /locus_tag="PL014_12380"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOT57933.1"
                     /translation="GGDEASASSAVMYAAVSTALCLALWWALPGARSTDDLVRTELIG
                     WRNATFLSFGILVGFGVVAVLPDGDARDVAVAYVDPVLMLIIGLAILPTPIRLLRTMS
                     RELLEMRPAADIEVPARAAVLETCWAAGLPDPVVRMTKTGGRLYVEVDHVVAPGTWDV
                     ADIDVLRRALVESLASERYTVWLNVDLSTDPAWQLS"
BASE COUNT          480 a         1143 c         1096 g          421 t
ORIGIN      
        1 ttccgcatcg cggtcgacct cgtcgacgaa ggcgtcatca ccgaggaaga agccctcctg
       61 cgcgtcaccg gggaccagct cgcgcagctg atgttcccgc acttcgaccc gcagggtgcc
      121 cgctcgctgc tcgcgaaggg catgagcgcg tcccctggcg cggccgtggg ggtcgccgtg
      181 ttcgactcgg aggccgcggt ccgcctggcc gacgccggca ccccggtcat cctcgtgcgc
      241 cgcgagacca accccgacga cctgcacggc atggtcgcgg cggccggcat cctcaccagc
      301 cgtggcggca agacgtccca cgcggcggtc gtagcccgcg gtatgggccg cacctgcgtg
      361 tgcggcgccg agaccctcga ggtcgacacc aggacccggc agttcaccac ccccgacggc
      421 accgtcgtgc gcgagggaga cacgatctcc atcgacggct ccaccggcga gatcttcgcc
      481 ggtgaggtcg ctgttcgccc ctcacccgtc gtgcggtact tcgagggaac catgagcgtc
      541 gaggagggtg gcccgctcgt ggccgccgtc gaccggctca tgacggtcgc ggaccgccac
      601 cgccgcatgc aggtgcgggc caacgccgac aacgccgagg atgccgcccg cgcccgccgt
      661 ttcggcgcac agggcatcgg cctgtgccgc accgagcaca tgttcctcgg tgagcggcgc
      721 gagctcgtcg agcgcctcat cctcgccgag ggcccggccg agcgccaggc cgtcttcggg
      781 gagctgctgc ccctgcagcg agccgacttc atcgagatct tcgaggcgat ggacgggctg
      841 ccggtcacgg tgcgtctgct cgacccgccg ctgcacgagt tcctccccga cttcaccgag
      901 ctgtcggtca aggtcgccgt cgccgaagcc accggccgtg agcacgacac cgactcgctg
      961 ctgctgctcg cggtgcaacg cctgcacgag gagaacccca tgctcgggct gcgtggtgtg
     1021 cgcttgggca tcgcgatccc ggggctgttc gagatgcagt cgcaggccat cctcgaggcg
     1081 gccgcgatcg tcgccgagcg gggcggccat gcgcgacccg agatcatggt cccgctcgtc
     1141 ggtgccgtcg aggagttgcg caccatccgt ctcgagatcg acgaggccgc gcacgaggca
     1201 cgggtcaagc acgccgtcga cctcgaacac ctcgtcggca cgatgatcga actaccccgg
     1261 gccgcgttga cggccgggga gatcgcccag gctgccgact tcttctcctt cggcaccaac
     1321 gacctcaccc agaccacgtg gggcttctcg cgcgacgacg tcgagtccag cttcttctca
     1381 cgctacatgg aggccggcat cctcgggtcg tcgccgttcg agaacatcga ccgtgacggc
     1441 gtcgggcgac tcgtgcgcat cggcaccgac gagggacggc gcaccaagcc ggggctcaag
     1501 gtcggcgtgt gtggcgagca cggcggcgac ccggcctcgg tgcacttctt cgaggagcgc
     1561 cggctcgact acgtctcgtg ctcaccgttc cgcgttccgg tggcgcggct cgaggcggcc
     1621 cgggctgtgc tgtccggccc ggcgtcctga gttaccccct ccccctcccc tccggccggg
     1681 cccgctcggc ccggtcgagg gtcaccttga cccggtggcc ggtgatgacc gcggcgagcc
     1741 aggccaggcc gacgagccag cccgccatga catcggtcag ccagtggtgg cccagccaca
     1801 cccgggacag ccccatggcc acgacgaaca cgacgacgac tgaggcgacg aggatcctgc
     1861 tgcgcaggtg ccggcgcccg agcatcacca ggtaggccga caggccgagc acgacggtgg
     1921 cgttcagggt gtgtcccgac gggaacgacg gcgacgtctc gaggggcggc accgcgtaga
     1981 tcaccggggg tcgcgcccgg gccgtgaggt ccttgcccag cacggtgatg agcaccgacc
     2041 cggctgcggc gatgagcatg aggaccaccg gcaaccagga ccggcgacgc acggcgagag
     2101 tgatgacgat cgccgcggcg atgagcggca tccccaccgt gccgccgagg tcggtgaagt
     2161 gggtgaccca ttgctcactg gcgggggtgc ggctgttcac ggcggcctcg aggaccggct
     2221 cgtcgagcgc ggccagcccg tcgttctcca ccaccgcctc gtagacctcg gccgagagca
     2281 cggtcgcccc gagcacgatg gccccgccga tgaccgtcgt gaggaccagg gcgaggttcg
     2341 ccagactcca gcgccagacg cggccgagca cgatgacgac gcgggcgatc gcggccacga
     2401 ggtgctggcc ggcccgggtg ggccactgcc cgaggtctcg atcaccgatc cgctcgagct
     2461 cggggggccc gctgccgggg tctgacggag gctggggcgt tgtcgcggcg ggcatacggg
     2521 cattgaacca ccgcggaatc aggtgggctc acgacagctg ccaggccgga tcggtcgaca
     2581 ggtcgacgtt gagccacacg gtgtaccgct ccgacgccag gctctcgacg agcgcgcggc
     2641 gcagcacgtc gatgtccgcg acgtcccacg tgcccggcgc gacgacgtgg tcgacctcga
     2701 cgtagaggcg tcccccggtc ttcgtcatcc ggaccaccgg atcgggcagg cccgccgccc
     2761 aacaggtctc gagcacagcg gcccgggcgg ggacctcgat gtcggccgcc ggccgcatct
     2821 ccaacagctc acgcgacatg gtgcgcaaca acctgatcgg ggtcggcaag atggccaggc
     2881 cgatgatcag catcaggacg gggtcaacgt aggccaccgc gacgtcgcgc gcgtcaccgt
     2941 cgggcagcac cgcgacgacg ccgaagccca ccagaatgcc gaaggacaag aaggtggcgt
     3001 ttcgccagcc gatgagctcg gtgcggacca ggtcgtcggt cgacctcgcg ccgggcagcg
     3061 cccaccagag cgccaggcac agcgcggtgg acacggcggc gtacatcacg gccgacgacg
     3121 ccgacgcctc gtcaccgccc
//
LOCUS       DAOSOC010000237         3120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_31252_length_3120_cov_6.464600, whole genome shotgun sequence.
ACCESSION   DAOSOC010000237 DAOSOC010000000
VERSION     DAOSOC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31252_length_3120_cov_6.464600"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PL014_12385"
     CDS             complement(<1..1048)
                     /locus_tag="PL014_12385"
                     /inference="COORDINATES: protein motif:HMM:NF025587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HOT57934.1"
                     /translation="MTLTPHRELTLPTIDLRSAAGAGAGPERADLLEQLRTAAHELGF
                     FYVAGHSIPAEVEADVLAAAQTFFALPETTRREISNLNSPHFRGYTALATEHTAGAAD
                     LREQIDIGPERPALDLGDGDPAYLRLVGPNQWPSEVPQLRDAVLRWLDEAGRVARSVL
                     ALLAESLGEDPTWFDQWFDDEAVTTTKLVRYPAPPAGEATRAATGQGVGSHKDYGWLA
                     LVLQDGQSGLQVRADDGSWLEATPVPGTLVFNVGEALEVATGGYLRANVHRVVAPTTG
                     VDRYSVPFFLGPRLDAVVEPLALPAVLAARARGVEQDPTNPIFAEYGRKALLGWLRRA
                     LRHLLEHGHRPHPGA"
     gene            1575..2615
                     /locus_tag="PL014_12390"
     CDS             1575..2615
                     /locus_tag="PL014_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT57935.1"
                     /translation="MNTHRFTLAAVAVSAAMVMSACGGGGASDTVGDSAAAEAPAGAE
                     GGTVNLFAYAVPKVGYDKLIPAFQASEAGKGVQFQQSYGASGDQSRKVEAGAEADFVN
                     FSVEPDITRLVDAGLVDAAWNANEHRGIPFGSVVTIVVREGNPKGIKDWDDLLESGVE
                     VVTPNPFSSGSAKWNLLAPYAAKSNGGVDQEAGLNYISSLVGDHVKIQPKSGREATEA
                     FLQGTGDVLLSYENEALFIERKGEPVEHVTPPQTFKIENPTAVISKGAGVKAATAFNE
                     FLYTPEAQRLWAEAGFRPVDATVAQEFAADFPQPEKLWTITDLGGWKQVDSTLFKKDV
                     GAIAKIYDAATE"
     gene            2621..>3120
                     /locus_tag="PL014_12395"
     CDS             2621..>3120
                     /locus_tag="PL014_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOT57936.1"
                     /translation="MTATTHRHDDVRALTSPVATTAGAPPAGEGHRPRRVPTRALGVG
                     VVGLWLSVIVLLPLAALTVASLEEGLAGFWDAVTAPAALGALRTTLLVSVVVALVNAV
                     MGTLIAWVLVRDEFPGQRIVNALIDLPFALPTIVASIVLLSLYGPNSPVGVQLNATRW
                     GLVVALA"
BASE COUNT          466 a         1050 c         1137 g          467 t
ORIGIN      
        1 cggcacctgg gtgaggtcgg tgaccgtgtt cgagtagatg ccgtagggct cgacgcagcc
       61 acccgaggag ggccttgcga ccgtactcgg cgaagatcgg gttggtgggg tcctgctcga
      121 cgccgcgggc tcgggcggcg agcacggcgg gcagggcgag gggttcgacg acggcatcca
      181 gccgtgggcc gaggaagaac ggcacggagt accggtccac cccggtggtc ggcgcgacca
      241 cgcggtgcac gttcgcgcgc aggtatccgc cggtggccac ctcgagggct tcgccgacgt
      301 tgaacaccaa ggtgccgggc acgggggttg cctcgagcca gctgccgtca tcggcccgca
      361 cctggaggcc ggactgtccg tcctggagca cgagggcgag ccagccgtag tccttgtggc
      421 tgccgacgcc ctggccggtg gcggcccttg tggcctcgcc tgccggtggt gcggggtagc
      481 gcacgagctt cgtggtcgtc acggcctcgt cgtcgaacca ctggtcgaac cacgtggggt
      541 cctccccgag ggactcggcg agcagtgcca gcacgctgcg cgccacccgc ccggcctcgt
      601 cgagccagcg aagcacggcg tcgcgcagct ggggcacttc gctcggccac tggttcgggc
      661 cgacgaggcg caggtaggcg gggtcgccgt cacccaggtc gagagccggg cgctcggggc
      721 cgatgtcgat ctgctcgcgc aggtcggcgg cgccggcggt gtgctcggtg gccagtgcgg
      781 tgtagccgcg aaagtgtggc gagttgaggt tggagatctc gcggcgggtg gtctctggca
      841 gggcgaagaa ggtctgcgcc gccgcgagga catcggcctc gacctcggcg gggatgctgt
      901 gcccggcgac gtagaagaaa ccgagctcgt gggctgcggt gcgcagctgc tccagcaggt
      961 cggcgcgctc gggaccggcg ccggcgccgg cggcgctgcg gaggtcgatc gtcgggagag
     1021 tgagctcgcg gtgcggggtg agggtcatcg tcgtccttgc ctttcgtgag gtgaggtggg
     1081 cagcagtcgg tggtgccttt gcgcaggggt gtgcgctgcg cgggggagtg ccgggcctgg
     1141 gggagtgccg ggcgcggcgg tgcggacacg ggcacggcgg tgggcacccc gtcggggtgc
     1201 accggcacga gctgacgagt ggtgcccggc cgcggctgca cgctggtggc tacggccagg
     1261 tcgccctgcg gtacccgtgt cgtcgcgggc gcttcgaggg cgtcgcagtc agcggcgaca
     1321 ggcgcgcgga cagcacattc gagcagcctg cagggtgtcg ccgggacggt gacgaaccct
     1381 actgatcgtt tggatgtgca taagagcacg ttatatgaat cgcacccctt cgccaagctg
     1441 cccacattgt gagacataac aaacagtaat tggatccata acagacaact atgcaaactc
     1501 ctacctgtca gataggacat gcgccgccgg gcgccaccgc ccgttccggg cctcacacag
     1561 gagctacaga caccatgaac acacaccgct tcaccctcgc cgccgtcgcc gtcagcgccg
     1621 cgatggtgat gagcgcctgc ggcggtggtg gcgcgagcga caccgtcggt gactcagccg
     1681 ccgccgaggc tccggccggc gcagagggcg gcacggtgaa cctcttcgcg tacgccgtcc
     1741 cgaaggtggg gtacgacaag ctcatccctg ccttccaggc gagcgaggcc ggcaagggcg
     1801 ttcagttcca gcagtcctat ggcgcctcgg gcgatcagtc ccgcaaggtc gaggccggcg
     1861 ccgaggcgga cttcgtgaac ttctccgtcg agcccgacat cacccgactc gtcgatgccg
     1921 ggctggtcga cgcggcgtgg aacgccaacg agcaccgcgg catcccattc ggctccgtcg
     1981 tgaccatcgt cgtgcgcgag ggcaacccca aggggatcaa ggactgggac gacctgctcg
     2041 agagcggcgt cgaggtcgtg acgccgaacc cgttcagctc cggctcggcc aagtggaacc
     2101 tgctggcgcc gtacgccgcc aagagcaacg ggggtgtcga ccaagaggcc ggcctgaact
     2161 acatcagctc gctcgtcggc gaccacgtga agatccagcc caagtccggc cgcgaggcga
     2221 ccgaggcctt cctccagggc accggagacg tcctgctcag ctatgagaac gaggccttgt
     2281 tcatcgagcg caagggtgag ccggtcgagc acgtcacccc gccgcagacc ttcaagatcg
     2341 agaacccgac cgcggtgatc agcaagggcg cgggcgtcaa ggcggcgacg gccttcaacg
     2401 agttcctcta cacgcccgag gcgcagcggc tctgggccga ggcagggttc cgccccgtcg
     2461 acgcgacggt cgcgcaggag ttcgccgccg acttcccgca acccgagaag ctgtggacca
     2521 tcacggacct cggcggctgg aagcaagtcg actcgaccct gttcaagaag gacgtcggcg
     2581 cgatcgcgaa gatctacgac gcggccaccg agtaggcacc atgactgcca ccacccatcg
     2641 ccacgacgac gtccgtgccc tgacgtcgcc cgtcgccacc acggccggtg cccctcccgc
     2701 tggggagggg caccgccccc ggcgcgtccc cacccgtgcc ctcggggtcg gcgtcgtggg
     2761 gctgtggctc agcgtcatcg tgctgctgcc gctcgccgcc ctgacggtcg cctcgctgga
     2821 ggagggtctc gccgggttct gggatgccgt gacggcgccg gccgcgctcg gggcgctgcg
     2881 caccacgctg ctcgtctccg tcgtggtcgc gctggtcaac gccgtcatgg gcacgctcat
     2941 cgcgtgggtc ctggtgcgcg acgagtttcc gggccagcgc atcgtcaacg ccctcatcga
     3001 cctgcccttc gccctgccga cgatcgtcgc gagcatcgtg ctgctctccc tctacgggcc
     3061 gaacagcccg gtcggcgttc agctgaacgc cacccggtgg ggcctggtcg tggcgctggc
//
LOCUS       DAOSOC010000238         3114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_31324_length_3114_cov_8.100033, whole genome shotgun sequence.
ACCESSION   DAOSOC010000238 DAOSOC010000000
VERSION     DAOSOC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31324_length_3114_cov_8.100033"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..2309)
                     /gene="tkt"
                     /locus_tag="PL014_12400"
     CDS             complement(102..2309)
                     /gene="tkt"
                     /locus_tag="PL014_12400"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT57937.1"
                     /translation="MAKKAGWTDLDVRAVDTARLLAADAVQKAGNGHPGTAMSLAPAA
                     YLLYQNVMNHDPSDPAWLGRDRFVLSCGHSSLTQYVQLFLSGYGLELKDLKALRTWGS
                     LTPGHPEVHHTKGVEITTGPLGSGLASAVGMAMAQRRQRGLFDPEAKAGESVFDHHVW
                     VLASDGDLMEGVAAEASSLAGHQQLGNLTVVYDANQISIEDDTDISFSEDVAMRFESY
                     GWEVIDVDWRRSSEPAGTADYTEDIDALLAALTKSRRSRTKPTLVRLHTVIAWPAPTV
                     RGTGKSHGSALGDAEIAATKELLGFDPAKSFEVDRAVLAHAREVKKRGKAAHKEWNTR
                     FTAWRKAQPERAALLARLVDGGLPSGFEKSLPVFDADPKGLATRAASGKVLGALAATL
                     PELWGGSADLAESNNTTMAGEPSFIPASRQTPAWKGGPYGRTLHFGIRENAMGMILNG
                     IALEGLTRPYGGTFLVFSDYMRPAVRLAAIQQLPVTFVWTHDSIGLGEDGPTHQPIEH
                     LASLRAMPGLAVVRPADANETAQAWAEILRRGTPAGLALSRQNLPVIDRTEHAKATGV
                     TKGAYVLVDAASGSPDVILVATGSEVSLALAARLELEKAGVATRVVSMPCREWFEQQG
                     AAYRNKVLPPSVRARVSIEAGVAQGWRDVVGDSGRCVSIEHFGASADAATLFRQFGFT
                     PEAVVKAAKDSLAAARKAGQVPGLTETAPRARKDTGTDVGTPKAASRKSAAGK"
     gene            2585..>3114
                     /locus_tag="PL014_12405"
     CDS             2585..>3114
                     /locus_tag="PL014_12405"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOT57938.1"
                     /translation="MANYVALTKPRIIELLLVTTVPVMFLAERGVPAVWLVVATLVGG
                     TLSAGAANTFNSVYDRDIDALMHRTSNRPMVTGAITTRNAVLFGAVLTVISTLWFALV
                     VNVLSALLSLAAIALYAVGYTILLKRRTSQNIVWGGIAGCMPTLIGWSAVTGTVGWPA
                     VVLFLVIFFWTPPHYWP"
BASE COUNT          410 a         1063 c         1149 g          492 t
ORIGIN      
        1 cgaggttgcc cgtggtcagg cgttcacggg agaggtcgtc cagccagacg gagacgccgt
       61 gggcggcgag gtcggagacg gcggcgctcg tcatgccgac ctcacttccc ggccgcgctc
      121 ttgcggctgg ctgccttggg ggtgccgacg tcggtgccgg tgtccttgcg ggcccggggt
      181 gcggtctcgg tcaggcccgg tacctggccg gccttgcgtg cggccgccag cgagtccttc
      241 gccgccttga ccacggcctc gggggtgaaa ccgaactgac ggaagagggt cgcggcatcc
      301 gccgaggcgc cgaagtgctc gatgctcacg cagcggccgg agtcgccgac cacgtcgcgc
      361 cagccctggg ccaccccggc ctcgatgctc acccgggccc ggaccgaggg tggcagcacc
      421 ttgttgcggt aggcggcgcc ctgctgctcg aaccactcgc ggcagggcat cgagacgacc
      481 cgggtcgcaa ccccggcctt ctccagctcc aggcgcgcag cgagggccag cgacacctcg
      541 gaacccgtgg cgacgaggat gacgtcgggg gagccggagg ccgcgtcgac gaggacgtag
      601 gcgcccttgg tgaccccggt ggccttcgcg tgctcggtgc ggtcgatgac cggcaggttc
      661 tgccgcgaca gggccagacc ggcgggggtg ccgcggcgca ggatctcggc ccacgcctgg
      721 gcggtctcgt tggcgtccgc ggggcggacc acggcgagcc cgggcatggc ccgcagcgag
      781 gcgaggtgtt cgatcggctg gtgggtcggg ccgtcctcgc ccagaccgat cgagtcatgg
      841 gtccacacga aggtcaccgg aagctgctgg atggccgcga gacgcacggc ggggcgcatg
      901 tagtcggaga acacgaggaa ggtgccgccg tacgggcggg tcagcccctc gagggcgatg
      961 ccgttgagga tcatccccat ggcgttctcg cggatgccga agtgcagggt gcggccgtag
     1021 ggcccgccct tccaggccgg cgtctggcgc gaggccggga tgaaggaggg ttcgccggcc
     1081 atcgtcgtgt tgttggactc ggcgaggtcg gccgacccgc cccacagctc ggggagggtt
     1141 gccgccaggg ccccgaggac cttgccggat gccgctcgcg tcgccaaccc cttggggtcg
     1201 gcgtcgaaga cgggcagcga cttctcgaag cccgacggca ggccgccgtc gacgagcctg
     1261 gccagcaggg cggcgcgctc gggctgggcc ttgcgccacg cggtgaaccg ggtgttccac
     1321 tccttgtggg cggccttgcc ccgcttcttg acctcccgcg cgtgggcgag caccgcccgg
     1381 tcgacctcga agctcttcgc cgggtcgaac ccgaggagct ccttggtcgc ggcgatctcg
     1441 gcgtcgccga gggccgagcc gtgggacttg ccggtgccgc ggacggtggg tgccggccag
     1501 gcgatgaccg tgtgcaggcg cacgagcgtg ggcttggtgc gcgagcggcg gctcttggtc
     1561 agcgccgcga gcagggcgtc gatgtcctcg gtgtagtcgg cggtgccagc aggctcggaa
     1621 ctccggcgcc agtcgacgtc gatgacctcc caaccgtagg actcgaaccg catcgcgacg
     1681 tcctcgctga acgagatgtc ggtgtcgtcc tcgatggaga tctggttcgc gtcgtacacg
     1741 acggtgaggt tgccgagctg ctggtggccc gcgagcgacg aggcctcggc agccaccccc
     1801 tccatgaggt caccgtcgga ggcgagaacc cacacgtggt ggtcgaagac gctctcgccg
     1861 gccttggcct ccgggtcgaa cagtccgcgc tggcggcgct gggccatggc catgccgacc
     1921 gccgaggcca gcccggagcc gagagggccg gtggtgatct cgacaccctt ggtgtggtgc
     1981 acctcggggt ggccgggggt cagtgacccc cacgtgcgca gcgccttgag gtccttgagc
     2041 tccaggccgt agccgctcag gaacagctgg acgtactggg tgagcgagga gtggccgcag
     2101 gagagcacga agcggtcacg ccccagccac gcgggatcgg acgggtcgtg gttcatgacg
     2161 ttctggtaga gcaggtacgc cgccggggcc aggctcatcg cggttcccgg gtgtccgttg
     2221 ccagccttct gcacggcatc ggcggccagc agcctggcgg tgtcgacggc ccggacgtcg
     2281 aggtcggtcc accccgcctt cttcgccacg ggggcagcga ggtcgctgct gcgggcgtcg
     2341 gtcggggtcg aggtgctgct ggcgggcttg ctggtcacag gagcttcttt cgcgaagacg
     2401 gtgcacacgg caggtcgagt cgtcggccag cctagtgtct gggcccgccc gcggttctcc
     2461 gtgctgccgg gcggacggct agaatcgccg gacaccctcc cgcacctgct ctaaggacga
     2521 cgtggcgacc attccgacga cgaccgactc cccgtcggtg ccgccgctcg agcgacgctc
     2581 cacggtggcc aactacgtcg cactgaccaa gccgcgcatc atcgagctgc tgctcgtcac
     2641 caccgtgccg gtgatgttcc tggccgagcg gggcgtcccc gcggtgtggc tcgtcgtcgc
     2701 gaccctcgtg ggtggcaccc tcagtgccgg cgcggcgaac accttcaact cggtctacga
     2761 ccgcgacatc gacgccctga tgcaccgcac gagcaaccgg ccgatggtca ccggtgcgat
     2821 cacgacgcgc aacgcggtgc tgttcggcgc cgtcctgacg gtcatctcga cgctctggtt
     2881 cgccctcgtc gtcaacgtcc tgtcggccct gctgtcgctg gcggcgatcg cgctctacgc
     2941 ggtcggttac acgatcctgc tcaagcgtcg tacgtcgcag aacatcgtgt ggggcggcat
     3001 cgccggctgc atgccgaccc tcatcgggtg gtcagccgtc accggtacgg tcggctggcc
     3061 cgcagtggtg ctcttcctcg tgatcttctt ctggaccccg ccgcactact ggcc
//
LOCUS       DAOSOC010000239         3091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_31614_length_3091_cov_6.952240, whole genome shotgun sequence.
ACCESSION   DAOSOC010000239 DAOSOC010000000
VERSION     DAOSOC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31614_length_3091_cov_6.952240"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PL014_12410"
     CDS             complement(<1..218)
                     /locus_tag="PL014_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57939.1"
                     /translation="MGDRTGIQGALHTRWAGSATRAGSGFLVAVAALGVAVAYVVRRQ
                     PQRALGVLGGRGPVLFGARSPERQVAIT"
     gene            389..1351
                     /locus_tag="PL014_12415"
     CDS             389..1351
                     /locus_tag="PL014_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57940.1"
                     /translation="MAAVEVRGLRKVYGEVRAVDGLDLTVDEGEVVALLGPNGAGKTT
                     TVEVLEGYRAPDSGSVEVLGFDPQTGGRAFRERIGIVLQEAGFEDTFTPRELVRLHAG
                     YYPHPRAVAEVLTLTGLDEKADARVRTLSGGQRRRLDLALGIVGDPELLFLDEPTTGF
                     DPSARRRAWELVESLRALGATILLTTHYLDEAEHLADRVVVIDHGRVLAEGTPEALAA
                     AAGAATVISFRMPSGLRVADLPGVGHEARVAGSMVEVRTDTPTADVAELATWASGRGI
                     ELEALALARPSLEDVYLDLVGEAGAVGEAGAVGEAVDADDGATR"
     gene            1348..3030
                     /locus_tag="PL014_12420"
     CDS             1348..3030
                     /locus_tag="PL014_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT57941.1"
                     /translation="MTAVATTPHPRRSAGGSGGSGPAMLLRQTSHELAALSRTPITLI
                     LSIGLPLLFFVLLSALVGNEVVDERGGVRVVQYLAPGMASFGVVMATFSFLAVGLAEA
                     RATGVVKRQAGTPAPRWVLVGGRIGAALVLGLTSTALVLATGVVLYDLQVPSRSVLSI
                     ITTLVFASGCFSALGLALAMALPTMQLTLAVSNGVVIPLAFISDMFMFSGDMPDWLET
                     IGWIFPLKHLTALFADALNPYLTGSGFQLDHLLVIALWGVAGAVVATVLLRRDRDRDA
                     SRATAAGRSRRSRAGSRGAAADARPRRTASPGAVALVSEQVAHTQSILWRDVSAVFFA
                     VAFPVLLVAIIPAVNGGGDVLMSDGAPLATFYAGTMAIYGAAVTAYVNMPQSLAEDRD
                     RGVLKRTLGTPLPAPALLLGRVVGALVVALVTGLAIVVLAAVAYRPPLPRGMPSAVVT
                     LVVATVCFAVVGLAVMTFVRSAQAVVGVTLGTLLPLAFISDVFVVGASFPPVLEAISW
                     FFPLRHAAAAMSQAVGPDVTGSGLALDHLAVLVAWTFAGAVVVALRFGRRVS"
BASE COUNT          361 a         1196 c         1068 g          466 t
ORIGIN      
        1 atggtgatcg ccacctgccg ctccggcgac cgcgcaccga acaggaccgg accccgaccg
       61 ccgagcacac ccaacgcccg ctggggctgc cggcgcacga cgtaggcaac cgcgaccccg
      121 agggccgcca ctgccaccag gaacccggac ccggcccgcg tggcactgcc cgcccaccgg
      181 gtgtggagcg cgccctggat gccggtccga tcgcccattc cggcctcctt cgcgctgctg
      241 atggggagca cgtcgctgtc gccatcatgc ggcacggccc gacgggagtg atcactcaca
      301 accgcgcgcc agggcatccc cttcggtgtt gacttgaggc gggtgccgtc gcgccggcgg
      361 cagccacccc agcgggaagg tcgggatcat ggccgcggtc gaggtcaggg gcctgcgcaa
      421 ggtctacggg gaggtcaggg ccgtagacgg cctcgacctc acggtcgacg agggtgaggt
      481 cgtggccctg ctcggaccca acggtgccgg gaagaccacc acggtcgagg tgctcgaggg
      541 ctaccgggcc ccggactcgg gctcggtcga ggtgctcggg ttcgacccgc agaccggggg
      601 ccgagccttc cgggaacgaa tcgggatcgt gctccaggag gctggcttcg aggacacctt
      661 cacaccgcgc gagctggtgc gcctgcacgc cggctactac ccgcacccgc gcgccgtcgc
      721 cgaggtgctc accctcaccg ggctggacga gaaggccgac gcccgggtgc ggaccctctc
      781 cggcgggcag cggcggcggc tcgaccttgc cctcggcatc gtcggcgacc ccgagctgct
      841 cttcctcgac gagcccacca cgggcttcga cccctcggcc cggcgccggg cctgggagct
      901 cgtcgagagc ctgcgcgcgc tgggtgccac gatcctgctc acgacccact acctcgacga
      961 ggccgagcac ctcgccgacc gcgtcgtggt catcgaccac ggccgcgtcc tggcagaggg
     1021 cacccctgag gcgctcgcgg ccgccgcggg cgccgcgacg gtcatctcct tccggatgcc
     1081 gtcgggcctc cgggtggcgg acctcccggg cgtgggccat gaggcccgcg tcgccggcag
     1141 catggtcgag gtgcgcaccg acacgcccac ggcggacgtc gccgagctgg cgacctgggc
     1201 gtccggccgg gggatcgagc tcgaggcgct cgccctggcc cgcccgagcc tggaggacgt
     1261 ctacctcgac ctcgtcggtg aggccggggc ggtcggtgag gccggggcgg tcggggaagc
     1321 cgtcgacgcg gacgatgggg cgacccggtg accgccgtcg cgaccacccc gcacccgcgc
     1381 cgctccgcgg gtggctcggg gggctccgga ccggcgatgc tgctgcgcca gaccagccac
     1441 gagctcgccg cgttgtcgcg caccccgatc accctgatcc tcagcatcgg gctgccactg
     1501 ctcttcttcg tgctgttgtc cgccctcgtc ggcaacgagg tcgtcgacga acgcggtggc
     1561 gtgcgggtcg tgcagtacct ggcacccggc atggcgtcct tcggcgtggt catggcgacg
     1621 ttctcgttcc tcgccgtcgg gctcgcggag gcccgcgcca ccggagtcgt gaagcgccag
     1681 gcgggcaccc ccgcgccccg gtgggtgctc gtcggcggcc gaatcggagc agcgctcgtg
     1741 ctcgggctca cctcgaccgc gctcgtcctc gccaccgggg tcgtcctcta cgacctgcag
     1801 gtgccgagcc ggtcggtgct ctcgatcatc accaccctcg tcttcgcctc cggctgcttc
     1861 agcgccctcg ggctcgccct ggccatggcc ctgcccacga tgcagctcac cctggccgtg
     1921 agcaacggcg tcgtcatccc gctcgccttc atctcggaca tgttcatgtt cagcggcgac
     1981 atgcccgact ggctcgagac catcggctgg atcttcccgc tcaagcacct caccgccctg
     2041 ttcgccgacg cactcaaccc ctacctcacc ggcagcgggt tccagctaga ccacctcctc
     2101 gtcatcgcgc tgtggggcgt cgccggggcc gtcgtcgcga cggtcctgct gcgccgggac
     2161 cgtgaccgtg acgcctcccg ggcgaccgcc gcgggccgct ccaggcgatc gagggccggg
     2221 tcccgcggcg cggccgccga cgcgagaccc aggcgcacgg catccccggg cgcggttgcc
     2281 ctggtctcgg aacaggtcgc acacacccag tcgatcctgt ggcgtgacgt gtccgccgtg
     2341 ttcttcgccg tcgcgttccc cgtgctgctc gtcgcgatca tccccgccgt caacggcggc
     2401 ggggacgtgc tgatgagcga cggcgcgccg ctcgccacgt tctacgcggg gacgatggcg
     2461 atctacggcg cagcggtcac cgcctacgtc aacatgcccc agagcctggc cgaggaccgt
     2521 gaccgcgggg tgctcaaacg aaccctgggc accccgcttc cggccccggc cctgctgctc
     2581 ggacgcgtcg tcggcgcact cgtcgtcgcc ctcgtcaccg ggctggccat cgtcgtgctc
     2641 gccgccgtcg cctaccggcc accgcttccc cgggggatgc cgtccgccgt cgtgaccctc
     2701 gtcgtggcca cggtgtgctt cgccgtcgtc gggctggccg tgatgacctt cgtgcgctcg
     2761 gcacaggccg tcgtcggggt caccctcggc accctgctcc cgctggcctt catctccgac
     2821 gtgttcgtcg tcggcgccag cttccccccg gtgctcgagg cgatctcctg gttcttcccc
     2881 ctgcggcacg cggcggcggc gatgtcccag gcggtcggcc ctgacgtcac gggctccggg
     2941 ctggcccttg accacctcgc ggtgctcgtg gcctggacgt tcgcgggggc ggtggtcgtg
     3001 gccctgcgct tcgggcgccg ggtgtcctga cacgcccggc gcggcgaacg cagtcgtcac
     3061 gcggcgttca cctcgtcacg ccagactggg c
//
LOCUS       DAOSOC010000240         3084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_31700_length_3084_cov_5.267085, whole genome shotgun sequence.
ACCESSION   DAOSOC010000240 DAOSOC010000000
VERSION     DAOSOC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31700_length_3084_cov_5.267085"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PL014_12425"
     CDS             <1..243
                     /locus_tag="PL014_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT57942.1"
                     /translation="DGVAASNLAGRTLADLVLGRQSAITALPWVGHRSPRWEPEPLRW
                     LGVNAGLRLAQAADREEAATGRPARLGWLLDRLTAH"
     gene            372..1286
                     /locus_tag="PL014_12430"
     CDS             372..1286
                     /locus_tag="PL014_12430"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOT57943.1"
                     /translation="MFPTDPGVPVNPYRATLYQPHELYAGLSENGYDATPDALAYHWS
                     RDGDSRHDAFVNLLRAIHDDSMSDALSEVIGTAAVVGVMGGHALERGTVGYTGAAHLG
                     HVLACAGFLVATGGGPGAMEAANLGAFAASEDAVADAMARLAGIPSFRPGIAPWAQVA
                     LDLRDDLLAARGGVGAVRSLGIPTWFYGHEPPNVFCDGIAKYFSNAIREDGLLARCDA
                     GIVVLPGAAGTVQEVFQVATRLYYGGEDAVLPPLVLVDSQHWTQTIPVWPALQALARG
                     GAMEGRLHLVDSPAEAAALVTSSAATAP"
     gene            complement(1323..1886)
                     /locus_tag="PL014_12435"
     CDS             complement(1323..1886)
                     /locus_tag="PL014_12435"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HOT57944.1"
                     /translation="MSLRDLRLRRGWSQEHLAEASGVSVRTIQRIEGGQPPSPFTVGA
                     LATALDVAPEAVSGASAHPGVDDRPRGDDDAPHVPAPEVSFPDAVRQAARRWGDFEGR
                     ASRSEFWYALLAVLVIVGALAALDERLGAAALTLFLIPLAAVGSRRLRDTAQSPWWLL
                     FVFAPMGFVIPFTLMAMPGKASGTDDS"
     gene            complement(1977..3083)
                     /locus_tag="PL014_12440"
     CDS             complement(1977..3083)
                     /locus_tag="PL014_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57945.1"
                     /translation="MPANLKSADAHPSTHASAPSATAEPVKETPLRKGERFQEIRLPA
                     KYTPKAPEGIGTDDYRCFLVDPGFDTDQLVSGIQVLLDNAPLVHHVVVSKVEPEDVTK
                     AEKIDAQTPGEGWTCFGGAGISGIAGGNLDKADWVGAWAPGGGERVMAEDIGIPLKAG
                     SQLVVQMHYNLLAGTGADRSAVLLRLSEAKGSDKQALQTMLLPAPVELPCRDNRTKGM
                     CSRTLAVADLKKRFAEDPATADLLHLLCGPLKPGPVQSCTRPIREEATIRAVSGHMHL
                     LGRAITVDVNKGTPKAKRVLDIPVWNFDDQGSIPLETPLDVGPGDEITVTCTHDQELR
                     DLLPAFEGTEDRYVAWGEGTTDEMCLGIVLSTPR"
BASE COUNT          409 a         1087 c         1132 g          456 t
ORIGIN      
        1 gacggggtgg cggcgagcaa cctggccggc cgcaccctgg ccgacctcgt gctcgggcgc
       61 cagtcggcga tcaccgccct gccgtgggtc gggcaccgtt cgccgcggtg ggagcccgag
      121 ccgttgcgct ggctcggcgt caacgccggg ctgcggttgg cgcaggccgc tgaccgcgag
      181 gaggcggcga ccgggcggcc ggcgcggctc ggctggctgc tcgaccggct caccgcccac
      241 tgacgctcac accgacccga ggtgggtgct gagctggcag ctccagcgag cacgtcagca
      301 cccgacggcg gttgggtcgg ctacgttcgg gggccgacct cgctcaacac ctgcgccagc
      361 acggggccct ggtgttcccg accgaccccg gtgtgccggt caacccctac cgggccacgc
      421 tctaccaacc gcacgagctc tatgccgggc tgtccgagaa cgggtacgac gccacgcccg
      481 acgccctggc ctaccactgg tcgcgcgacg gcgactcgcg ccacgacgcc ttcgtcaacc
      541 tgctgcgggc catccacgac gactcgatga gcgacgcgct gagcgaggtc atcggcaccg
      601 ccgcggtcgt cggtgtcatg ggtgggcacg cgctcgaacg gggaacggtg ggctacaccg
      661 gggccgccca cctcggccac gtcctggcct gcgccgggtt cctcgtcgcc accgggggtg
      721 ggccgggtgc gatggaggcg gccaacctgg gcgcgttcgc ggcatccgag gacgccgttg
      781 ccgacgccat ggcgaggctg gccggcatcc cgtcgttccg ccccgggatc gcaccgtggg
      841 cccaggtggc gctcgacctg cgcgatgacc tgctcgccgc gcgcggtggc gtgggcgcgg
      901 tgcgcagcct cggcatcccc acgtggttct acggccacga gccgcccaac gtcttctgcg
      961 acggcatcgc gaaatacttc tccaacgcga tccgcgagga cggcctgctc gctcgctgcg
     1021 acgccggcat cgtcgtgctg ccgggggcgg cagggacggt gcaggaggtc ttccaggtcg
     1081 cgacccggct gtactacggc ggcgaggatg ccgtgctgcc gcctctcgtg ctcgtggact
     1141 cccagcactg gacgcagacg atccccgtgt ggccggctct tcaggccctg gcccgcgggg
     1201 gtgcgatgga ggggcggctg cacctcgtcg actcccccgc cgaggctgcg gctctcgtca
     1261 cctcgtcggc cgccacggcc ccgtgacatg caggggacgc ggcatgtcgc ccggtcggca
     1321 tgtcaggagt catccgtgcc ggaggccttt cccggcatcg ccatgagggt gaacgggatg
     1381 acgaagccca tcggggcgaa cacgaacagc agccaccagg ggctctgggc ggtgtcgcgc
     1441 aggcgacggg agcccacggc tgcgagcggg atgaggaaca gcgtgagcgc ggcggcgccg
     1501 aggcgttcgt cgagcgcagc gagcgcaccg acgatcacga gcactgcgag cagcgcgtac
     1561 cagaactccg aacggctggc ccggccctcg aagtctcccc aacggcgggc cgcctgtcgc
     1621 acggcatccg gaaacgacac ttccggggct ggcacgtgcg gtgcgtcgtc gtcgcccctt
     1681 gggcggtcgt cgactcccgg gtgagcgctg gccccggaaa cggcttcggg cgcgacgtcc
     1741 agtgcggtgg cgagggcgcc gacggtgaac ggactcggcg gttggccacc ctcgatccgc
     1801 tggatggtgc gcacgctgac tccactggcc tcggccaggt gctcctggct ccagccgcgg
     1861 cggagccgaa ggtctcgaag actcacggca ccagtgtcac cccacaccgg gcgccggcac
     1921 cacgacacgg agccgccaaa ggcccgccac cgcgaggggg cggcgcgctc gaggcgtcag
     1981 cgcggggtgc tgaggacgat gccgaggcac atctcgtcgg tggtgccttc accccaggcg
     2041 acgtagcggt cctcggtgcc ctcgaacgcg ggaagcaggt cgcgcagctc ctggtcgtgg
     2101 gtgcaggtca ccgtgatctc gtccccgggc ccgacgtcca gcggggtctc caaggggatg
     2161 ctgccctggt cgtcgaagtt ccagacgggg atgtccagga cccgcttggc cttcggtgtc
     2221 cccttgttga cgtcgacggt gatcgcccgc ccgagcaggt gcatgtgccc ggagaccgcg
     2281 cggatcgtcg cctcctcacg gatcgggcgg gtgcagctct gcaccggccc gggcttcaac
     2341 ggcccgcaca gcaggtgcaa caggtcggcg gtggccgggt cctcggcgaa acgcttcttg
     2401 aggtcggcca ccgcgagggt gcggctgcac atccccttgg tgcggttgtc acgacagggc
     2461 agctcgacgg gagcgggcag gagcatcgtc tgcagtgcct gcttgtcgct gcccttggcc
     2521 tccgacagcc gcaggagcac cgcactgcga tcggcgccgg tgcctgcgag gaggttgtag
     2581 tgcatctgga cgacgagctg cgagcctgcc ttgagcggga tgccgatgtc ctcggccatg
     2641 acccgctctc ccccaccggg cgcccaggcg ccgacccagt cggccttgtc gaggttgccg
     2701 ccggcgatgc ccgagatgcc cgcgccaccg aagcaggtcc agccctcgcc cggggtctgt
     2761 gcgtcgatct tctcggcctt ggtgacgtcc tccggctcga ccttggagac aacgacgtgg
     2821 tgcaccagcg gtgcgttgtc gaggaggacc tggatgccgc tgacgagctg gtcggtgtcg
     2881 aagccggggt cgacgaggaa gcagcggtag tcgtcggtgc cgatgccctc gggggccttc
     2941 ggggtgtact tcgccggcag acggatctcc tggaagcgct cgcccttgcg cagaggcgtc
     3001 tccttgaccg gctccgccgt ggcactcggc gcgctggcgt gggtgctcgg gtgcgcgtcg
     3061 gcggacttca ggttcgccgg cagg
//
LOCUS       DAOSOC010000241         3077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_31779_length_3077_cov_9.466248, whole genome shotgun sequence.
ACCESSION   DAOSOC010000241 DAOSOC010000000
VERSION     DAOSOC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31779_length_3077_cov_9.466248"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="PL014_12445"
     CDS             <1..110
                     /locus_tag="PL014_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT57946.1"
                     /translation="VVELYISYLRKKIDAGRDPMIHTMRGVGYVLKATD"
     gene            128..1600
                     /locus_tag="PL014_12450"
     CDS             128..1600
                     /locus_tag="PL014_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT57947.1"
                     /translation="MPSPPSRWTLQAKLLASVLALFSVIMLATGALTLLETQRYLERG
                     LVSDLSTVLSRVDVDPDRDGRTLGQRGRGDGPAGAPGGGDVLVLGLNADGTVATTASG
                     VAINAVVNEDRQDDVLDSNQVRRIAEADVGTQPRRVEIGGDVGPYLLKADTLPGGSLL
                     IVGVSTHPVDELLAKLLALVAGGTVLGLVAVGVGGTVLIRRSLAPLDRVAATARRVSG
                     LKLDSGDVALAERVPAADADPHTEVGQVGLALNTMLDNVESALHARQESETQVRQFVA
                     DASHELRTPLASIRGYAELTRRESEPVPPTVTHAIGRVESEALRMQVLVEDMLLLARL
                     DAGRPLEREPVDLSLLAVNAVSDAHAASPDHVWELDLPEEPVEVPGDQARLHQILANL
                     LANARTHTPAGTRVVTRVRPEGELVRISVTDDGPGVPESVQATVFERFTRGDDARTRG
                     GGSTGLGLSIVAAVGQAHGGRVEMSSRPGDTTFSVLLPAT"
     gene            complement(1666..2157)
                     /locus_tag="PL014_12455"
     CDS             complement(1666..2157)
                     /locus_tag="PL014_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT57948.1"
                     /translation="MPLTIRRARPDELDAVGRLTVDAYVSGGVIESGSPYLSFLADAG
                     ARASDAQVWVAVDDRGIVGTVTYVDPGSPLAEVATQGEAEIRTLAVAPAASGEGIGEA
                     LTRAMIDLARDGGFGSVVLSSSTTMRAAHRLYERLGFVRLPERDWTPGRGIQLIAYEL
                     PLA"
     gene            complement(2217..2627)
                     /locus_tag="PL014_12460"
     CDS             complement(2217..2627)
                     /locus_tag="PL014_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57949.1"
                     /translation="MALVVAGLAILGQLATMVLPMLFFGFFGLFGFTDDFDEGFGSSG
                     VVTIGNGQAKLAADRTVTGPDLTRAVRDLIGPDDPFLPPIENITCDPAPPVSADVSVL
                     CRAGAGEGWVIVRFADATGEFEAVVVPGEDMMMP"
     gene            2858..2950
                     /locus_tag="PL014_12465"
     tRNA            2858..2950
                     /locus_tag="PL014_12465"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2894..2896,aa:Ser,seq:gct)
BASE COUNT          449 a         1109 c         1086 g          433 t
ORIGIN      
        1 acgtcgtcga gctctacatc tcctacctgc gcaagaagat cgacgccggc cgcgacccga
       61 tgatccacac gatgcgcggc gtgggctacg tgctcaaggc caccgactga tccggcgggc
      121 tgaccggatg ccgtcccctc cctcccgctg gacgctgcag gccaagctgc tcgcgtcggt
      181 gctggcgctg ttctccgtca tcatgctggc gaccggcgcg ctgaccctgc tcgagaccca
      241 gcgctacctc gagcggggcc tggtctccga cctgtcgacc gtcctctctc gtgtcgacgt
      301 cgaccccgac cgcgacggcc gcaccctcgg gcaacggggc cggggcgacg gaccggcggg
      361 ggcgcccggc ggtggggacg tgctcgtgct gggactgaac gccgacggca ccgtcgcgac
      421 aacggcatcc ggggtcgcca tcaacgccgt ggtcaacgag gaccgtcagg acgacgtgct
      481 cgacagcaac caggtccgcc gcatcgccga ggcggacgtc ggcacgcagc cacgccgggt
      541 cgagatcggt ggggacgtcg gcccgtacct gctcaaggcc gacacgctgc ccggtgggag
      601 cctcctcatc gtcggggtgt ccacacaccc ggtcgacgag ctgttggcga agctgctcgc
      661 cctcgtggcg ggtgggaccg tgctcgggct cgtcgcggtg ggcgtcggcg gcacggtgct
      721 gatccggcgc agcctcgcgc cgctcgaccg ggttgccgcc accgcgcgac gcgtctccgg
      781 gctcaagctc gactcgggcg acgtggcgct ggccgagcgc gtccccgccg cggatgccga
      841 cccgcacacc gaggtcgggc aggtgggtct cgccctgaac accatgctcg acaacgtcga
      901 gtccgcgctg cacgcgcggc aggagagcga gacgcaggtg cgccagttcg tcgcggacgc
      961 cagccacgaa ctgcgcaccc cgctggcgtc catccgcggg tacgccgaac tcacccgtcg
     1021 cgagagcgag ccggtgccac cgaccgtcac ccacgccatc gggcgggtgg agtccgaggc
     1081 gctgcggatg caggtgctcg tcgaggacat gctgctgctc gccagactgg atgccgggcg
     1141 gcccctcgag cgcgaacccg tcgacctgtc gctgctcgcc gtcaacgcgg tcagtgacgc
     1201 gcacgccgcc tcccccgatc acgtctggga actcgacctg cccgaggagc ccgtcgaggt
     1261 gcccggtgac caggcgcggc tgcaccagat cctcgccaat ctgctcgcca acgcccgcac
     1321 ccacaccccg gcggggactc gcgtggtcac ccgggtgcgc ccggagggtg agctcgtgcg
     1381 gatctcggtg accgacgacg ggcccggcgt gcccgagtcg gtgcaggcca ccgtgttcga
     1441 acggttcacc cgcggggacg acgcccgcac ccggggcggc ggctcgaccg ggctgggcct
     1501 cagcatcgtc gcagcggtcg gccaggccca cggtgggcgc gtcgagatgt cgagccggcc
     1561 gggtgacacg acgttctcgg tgctgctccc cgccacctga gtccttcgtc cgtcgtgagt
     1621 gcggaagtgg ttgccccagc cgccacttcc gcactcacgg cgtgatcagg cgagcggcag
     1681 ctcgtaggcg atgagctgga tgccgcggcc cggggtccag tcgcgctctg gcagtcgcac
     1741 gaacccgagc cgctcgtaca gccggtgcgc cgcccgcatg gtcgtcgacg acgacagcac
     1801 caccgacccg aagccaccat cgcgggcgag gtcgatcatc gcccgggtca gcgcctcccc
     1861 gatgccctca ccgctcgcgg cgggggcgac ggccagggtg cggatctcgg cctcaccctg
     1921 tgtggcgacc tcggccaacg gtgacccggg gtcgacgtag gtgaccgtgc cgacgattcc
     1981 ccgatcgtcc acggcaaccc acacctgggc gtccgaggcc cgcgcaccgg catccgcgag
     2041 gaacgacagg tacggcgacc cggactcgat gacgccaccc gagacgtagg cgtcgacggt
     2101 gagtcgaccc acggcatcca actcgtcggg gcgggcgcgg cggatcgtca ggggcacgca
     2161 gacatcctcg caaacggtcg cgctggcctc ggcagggcca gggcgaccgt ggcggtttac
     2221 ggcatcatca tgtcttcgcc cggaacgacg acggcctcga actcgcccgt cgcgtcggcg
     2281 aacctcacga tgacccagcc ctcgccggcg ccggcccggc acaggacgct cacgtcggcg
     2341 ctgacggggg gcgcagggtc acaggtgatg ttctcgatgg gcggcaggaa cgggtcgtcc
     2401 ggcccgatga gatcacgcac cgctcgggtg aggtcggggc cggtcaccgt gcggtcggct
     2461 gccagcttgg cctggccgtt gccgatcgtg accaccccgg aagacccgaa cccctcgtcg
     2521 aagtcgtcgg tgaagccgaa gaggccgaaa aatccgaaga acagcatcgg cagcaccatc
     2581 gtggcgagct gcccgaggat cgcaagccct gcgaccacga gcgccacgat ggcgatggtg
     2641 cgcgtcgagt cctgcttcgg ggcgggtggt gccgcctgct gatggacagg tgtcgcggtc
     2701 tgctgggtgg caggtggcga tgtcggtcct gctggatccc ccgtgtggct catggctgga
     2761 gtctgacagc aaccgcggcc gtcggcaggt ccaaaacgag accagcaccg ggtcgctttg
     2821 cccgccgggt gcccggccag ttaccctgtg cgacgttgga ggcgtcgcct agtccggtct
     2881 atggcgccgc actgctaatg cggtttgggt ctcaagccca tcgagggttc aaatccctcc
     2941 gcctccgcca caacgacaac gccccgcgag agcggggcgt tgtcgttgtg gcggagtgca
     3001 gagggatgct gggagcgggc gtggtcgggt gagggacgaa cccgcccacc ccgcggctcc
     3061 ctccgcctcc gcggctc
//
LOCUS       DAOSOC010000242         3043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_32271_length_3043_cov_9.568273, whole genome shotgun sequence.
ACCESSION   DAOSOC010000242 DAOSOC010000000
VERSION     DAOSOC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32271_length_3043_cov_9.568273"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PL014_12470"
     CDS             <1..1091
                     /locus_tag="PL014_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HOT57950.1"
                     /translation="RWWSPIINAEHLQMRETAGLVDLSAFVVFDVIGPQALAAVQNII
                     VAQADVKIGRVVYTPVLDETGGFRSDLTVMRLAHDHFRVVTGGAHGMADKKWFSDRMP
                     DGAVVVDLTSSWTTIGIWGPKAREIVSAITDSDMSHQGFPFGTCREIEVGSLHVLASR
                     ISYVGEYGWELYTPMEAGAALWDRLLEVGTPLGMVPVGIGVYGTTGRLEKGYRAYGAE
                     LDSERTLMETGMSRAKWKDQDFVGKDAVIAQAQEAPKAVLCTLTVDDHTSASGVKRYM
                     LGGEPILTRDGGILTDGHGHHPYVTSAGSAPSLGKHLLMAFLPPEQAVVGTELAVSYM
                     EELYPVTVAAADATPLFDPENARIRGLA"
     gene            1088..1861
                     /locus_tag="PL014_12475"
     CDS             1088..1861
                     /locus_tag="PL014_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT57951.1"
                     /translation="MTNILVCVKRAPDPTGEVVLTDDGLRVDGRYAGYTTSAHEESAV
                     ALAVQLTEGSDAMVTVLSVGSQDSIEQIRAGIAVGATDGILVEADADLLGPADVADAI
                     AQAVRAREEAGTTYDLVLLGNDAADTGDFQVGVRLAYLLDRPVAAGIQQVEVSDGRAT
                     LRGEGPDGTEVFEVDLPAVVTVLEGGVAPKYPSVMGRMRAKKAPVETVAWAGSAAGSG
                     RQVLTVPEAPPSQVTVIGEGAAAAPALVSVLRELGVVSR"
     gene            1858..2817
                     /locus_tag="PL014_12480"
     CDS             1858..2817
                     /locus_tag="PL014_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT57952.1"
                     /translation="MTVLVLIEVEGGSASLVSREALTFARGLGAGDLHAVVVGTPAAG
                     VVADCAEAGVVAIHAAQDPALERGYAAAAWAAVVEAAGRAARADLVVAPGTGRGNEVL
                     AHVAARRNLRMAANVVSVESLNPLVVQRQVVGGAALERATVDSRVRLMTMAGHAVEPV
                     AAPSPGAATVTPLAITVDPKDLRAQVVRTEVRETGDATGLTAARVVVGGGRGAGGPEG
                     FGGVEALANRLGGAVGVSRVVTSLGWRPHHEQVGQTGSRIAPDLYIACGISGAIQHWA
                     GCASSKTIIAVNTDPDAPMVTKANYAVIGDMHEVLPAVLDELA"
     gene            complement(2832..>3043)
                     /locus_tag="PL014_12485"
     CDS             complement(2832..>3043)
                     /locus_tag="PL014_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOT57953.1"
                     /translation="AGLEVDHRDHPADAVPHLADLARVLGLVATGSSDYHGTGKLNRI
                     GERTTSPAALEVIEALGTGTPVITP"
BASE COUNT          407 a         1140 c         1066 g          430 t
ORIGIN      
        1 cgcgctggtg gtcgccgatc atcaacgccg agcacctgca gatgcgcgag accgcgggcc
       61 tggtcgacct gtctgccttc gtcgtcttcg acgtcatcgg cccccaggcg ctggccgcgg
      121 tgcagaacat catcgtcgcc caggccgacg tgaagatcgg ccgcgtcgtc tacaccccgg
      181 tcctcgacga gaccggtggc ttccgctccg acctcacggt catgcgcctg gcccacgacc
      241 acttccgcgt ggtcaccggc ggcgctcacg gcatggccga caagaagtgg ttcagtgacc
      301 ggatgccgga cggcgccgtc gtcgtcgacc tcacgtcgag ctggacgacc atcggcatct
      361 ggggccccaa ggcccgcgag atcgtctctg ccatcaccga ctccgacatg tcgcaccagg
      421 gcttcccgtt cggcacgtgc cgcgagatcg aggtcggctc gctgcacgtc ctcgcgtcgc
      481 ggatctccta cgtcggtgag tacggctggg agctctacac cccgatggag gccggcgccg
      541 ccctgtggga tcgactgctc gaggtcggca cgccgctggg catggtcccc gtcggcatcg
      601 gtgtctacgg caccaccggc cgcctcgaga agggctaccg cgcctacggc gccgagctcg
      661 acagcgagcg caccctcatg gagaccggca tgtcccgggc gaagtggaag gaccaggact
      721 tcgtcggcaa ggacgccgtc atcgcccagg cccaggaggc ccccaaggcg gtgctgtgca
      781 ccctgaccgt cgatgaccac acctccgcca gtggcgtgaa gcgctacatg ctcggcggcg
      841 agccgatcct cacccgggac ggcggcatcc tcaccgacgg ccacggccac cacccctacg
      901 tgacgtccgc gggctcggct ccgagcctgg gcaagcacct gctcatggcc ttcctgccac
      961 ccgagcaggc cgtcgtcggc accgagctcg ccgtctccta catggaggag ctctacccgg
     1021 tcacggtggc cgctgccgac gcgacgccgc tgttcgaccc ggagaacgcc cgcatccggg
     1081 ggctggcgtg accaacatcc tcgtctgcgt caagcgcgcg ccggacccca ccggtgaggt
     1141 cgtgctcacc gacgacggcc tgcgcgtcga cggccgctac gccggctaca cgacgagcgc
     1201 ccacgaggag tccgcggtgg cgctcgcggt gcagctcacc gagggcagcg atgccatggt
     1261 gacggtgctg tcggtcgggt cgcaggacag catcgagcag attcgtgcgg gcatcgcggt
     1321 cggtgccacc gacggcatcc tcgtcgaggc cgacgccgac ctgctcggcc ctgccgacgt
     1381 cgccgacgcc atcgcccagg cggtgcgtgc ccgtgaggag gccggaacca cctacgacct
     1441 ggtcctgctc ggcaacgacg ccgccgacac cggcgacttc caggtggggg tccgtctggc
     1501 ctacctgctc gaccgtcctg tcgcggccgg catccagcag gtcgaggtca gcgacggccg
     1561 cgccacgttg cgcggggagg gcccggacgg caccgaggtg ttcgaggtcg acctgcccgc
     1621 ggtggtcacc gtgctcgagg gcggggtcgc gccgaagtac ccgagcgtca tggggcgcat
     1681 gcgcgccaag aaggcccccg tcgagacggt ggcctgggcc gggtccgccg ccggctccgg
     1741 ccgccaggtg ctcacggtgc ccgaggcgcc gccgagccag gtcaccgtca tcggtgaggg
     1801 tgccgcggcc gcgcctgccc tcgtctccgt cctgcgcgaa ctgggagtgg tgtcgcgatg
     1861 actgttctcg tcctgatcga ggtcgaggga gggtctgcct cactggtgtc ccgcgaggcg
     1921 ctgaccttcg cccgtggcct gggtgccggc gacctgcacg cggtcgtcgt cggcacgccc
     1981 gccgcgggtg tcgtcgccga ctgcgccgag gccggtgtcg tcgccatcca cgcggcgcag
     2041 gaccccgcgc tcgagcgggg atacgccgcg gccgcgtggg ccgctgtcgt cgaggcagcc
     2101 gggcgagccg cccgagccga cctcgtcgtc gcccccggca ccggccgcgg caacgaggtg
     2161 ctggcgcacg tcgccgcccg ccgcaacctg cggatggccg ccaacgtcgt ctccgtcgag
     2221 tcgctgaacc cgctcgtggt ccagcgtcag gtcgtcgggg gcgccgccct cgaacgggcc
     2281 accgtcgaca gccgggtccg cctcatgacg atggccggtc acgccgtcga gcccgtcgcc
     2341 gccccctccc ccggtgcggc gaccgtcacg ccgctcgcga tcaccgtcga ccccaaggac
     2401 ctgcgcgccc aggtggtgcg caccgaagtc cgtgagaccg gcgacgccac cggcctcacc
     2461 gccgcgcggg tcgtcgtcgg cggcgggcgt ggcgccggtg ggcccgaggg tttcggcggg
     2521 gtcgaggcct tggccaaccg cctcggcggc gcggtcggcg tctcgcgggt cgtcacgtcc
     2581 ctgggctggc gcccgcacca cgagcaggtc ggccagaccg gttcgcgaat cgcgccggac
     2641 ctctacatcg cctgcggcat ctcgggcgcc atccagcact gggccggctg cgcgtcgagc
     2701 aagacgatca tcgccgtcaa caccgacccc gacgccccga tggtcaccaa ggcgaactac
     2761 gccgtcatcg gtgacatgca cgaggtgctg cccgccgtgc tggacgaact ggcctgacct
     2821 ccgcgcggag gtcagggggt gatgaccggc gttccggtcc cgagggcctc gatgacctcc
     2881 agggcagccg gtgacgtcgt gcgctcgccg atccggttga gcttgccggt gccgtggtag
     2941 tcgctggacc ccgtggcgac caacccgaga acccgcgcga ggtccgcgag gtgcggcacg
     3001 gcatccgcgg ggtggtcgcg gtggtccacc tcgagacccg cca
//
LOCUS       DAOSOC010000243         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_32819_length_3003_cov_10.169946, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000243 DAOSOC010000000
VERSION     DAOSOC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32819_length_3003_cov_10.169946"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PL014_12490"
     CDS             <1..257
                     /locus_tag="PL014_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57954.1"
                     /translation="FVVGHLVGMVLVAIAVVRARVVAWWVGALIAVAQPIHVVSAVIV
                     PNRMLDVVLGWGATTLGYAIVALAVLRTSDDEWDLPPRSR"
     gene            356..1933
                     /gene="guaB"
                     /locus_tag="PL014_12495"
     CDS             356..1933
                     /gene="guaB"
                     /locus_tag="PL014_12495"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT57955.1"
                     /translation="MSLGLDEIGTTHDRDSSSQGSDLPSAAPHSPFAELGLTYDDVLL
                     QPGETDVIPSEVDTTARLTREISIRVPLVSAAMDTVTEARMAIAMARHGGLGVLHRNL
                     SIEDQAYQVDLVKRTQTGMISNPVTIGPDATLEELDDICGRYRVSGLPVIDESQRLLG
                     IVTNRDLRFTPVAEWADTIVRDVMTPMPLITGHVGISHDEATLLLRQHKRERLPIIDE
                     QGRLQGLITVKDFVKSEQFPNASKDADGRLLVGAAVGYFGEAWQRATTLVDAGVDVLV
                     VDTAHGHARLLLEMIERVKRDPATRHVQVIGGNVATRAGAQALIDAGADAVKVGVGPG
                     SICTTRVVAGVGVPQVTAIYQASLACAPAGVPLIGDGGLQYSGDIAKALVAGADTVMI
                     GSLLAGCEESPGELVFINGKQFKAYRGMGSLGAMSSRGKKSYSKDRYFQADVTSDDKI
                     VPEGIEGQVAYRGPVGAVAHQLIGGLHQSMFYVGARTVPELKARGRFIRITPAGLRES
                     HPHDITGIVEAPNYSQG"
     gene            complement(1936..2961)
                     /locus_tag="PL014_12500"
     CDS             complement(1936..2961)
                     /locus_tag="PL014_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOT57956.1"
                     /translation="MQPGPDDAITDIDGVLVGHCTREEPGWLTGVTVVVAPPGTVGGV
                     DVRGGGPGTRETDLLDPRTLVDAVDAVVLAGGSAFGLAAADGVSTGLYAAGRGWPVGP
                     GSHERVPIVPAAILFDLGRGGTWLNHPRPGDGAAAFAAAGAGPVEQGSVGAGTGARTG
                     GLRGGVGTASAVLSSGVTVGALVVVNAVGEPFGPDGRMLAAHLAADLDLPTPDAGLVT
                     SYREARAAAVAALRAGTATTLAVVATDATLSKAGCSKLAGVAHDGFARALSPVHTAFD
                     GDTVFALATGARPGVSGLDEVDLQTAAADCVARAIVRGVLAATSVDRTAEGGLALASY
                     RDAIIGD"
BASE COUNT          427 a         1157 c         1031 g          388 t
ORIGIN      
        1 ccttcgtcgt gggccacctc gtcggcatgg tgctcgtcgc catcgcggtg gtccgggccc
       61 gcgtcgtcgc ctggtgggtg ggcgccctca tcgccgtggc gcagccgatc cacgtggtct
      121 cggccgtcat cgtgccgaac cggatgctcg acgtcgtcct cggttggggc gcgaccacgc
      181 tcgggtacgc catcgtcgcc ctggccgtgc tgcgcacgag cgacgacgag tgggacctgc
      241 cgccccgcag ccgctgaccc cgcaacgtcc ggcagggtgg tcgctcgcgc gaccaccctg
      301 ccggacgttg cgaagcacgg caggggccct gcggatcagc acgtagaatc gggtgatgag
      361 cctcggactc gacgagatcg gcaccaccca cgaccgcgac agcagcagcc agggcagcga
      421 cctgcccagc gcagcgccgc actcaccctt cgccgaactc ggcctcacct atgacgacgt
      481 cctgctccag ccgggcgaga ccgacgtcat cccgagcgag gtcgacacca cggcccggct
      541 gacgcgcgag atctccatcc gcgtcccgct cgtctcggcg gcgatggaca ccgtgaccga
      601 ggcccgcatg gcgatcgcga tggcccgcca cggcggcctc ggggtgctgc accgcaacct
      661 ctcgatcgag gaccaggcct accaggtcga cctcgtcaag cgcacccaga ccggcatgat
      721 ctccaacccg gtgaccatcg gccccgacgc gaccctcgag gagctcgacg acatctgcgg
      781 tcgctaccgc gtctcgggcc tgcccgtcat cgacgagtcg cagcgcctgc tcggcatcgt
      841 caccaaccgc gacctgcgct tcaccccggt cgccgagtgg gccgacacca tcgtgcgtga
      901 cgtcatgacg ccgatgccgc tcatcaccgg tcacgtcggc atcagccacg acgaggccac
      961 cctcctgctg cgccagcaca agcgcgagcg cctgccgatc atcgacgagc aggggcgcct
     1021 gcaggggctc atcaccgtca aggacttcgt gaagtccgag cagttcccga acgccagcaa
     1081 ggacgccgac gggcgcctgc tcgtcggcgc ggccgtcggg tacttcgggg aggcctggca
     1141 gcgcgcgacg accctcgtgg atgccggggt ggacgtcctc gtcgtcgaca ccgcccacgg
     1201 tcacgcccgt ctcctgctcg agatgatcga gcgggtcaag cgtgacccgg cgacccgcca
     1261 cgtgcaggtc atcggcggca acgtcgcgac ccgggcgggg gcacaggcgc tcatcgacgc
     1321 cggtgccgat gccgtcaagg tcggggtcgg ccccgggtcg atctgcacca cgcgcgtcgt
     1381 cgcgggcgtc ggcgtcccgc aggtgaccgc catctaccag gcgtcgctcg cgtgtgcccc
     1441 ggccggtgtg ccgctcatcg gggacggtgg cctgcagtac tccggcgaca tcgccaaggc
     1501 cctcgtggcc ggtgccgaca cggtcatgat cggctcgctg ctggccggct gcgaggagag
     1561 cccgggcgag ctcgtcttca tcaacggcaa gcagttcaag gcctaccgcg gcatgggctc
     1621 gctcggcgcg atgtcgtcac ggggcaagaa gtcgtactcc aaggaccgct acttccaggc
     1681 cgacgtcacc tccgacgaca agatcgtccc cgagggcatc gagggccagg tggcctaccg
     1741 cggccccgtc ggggcggttg cccaccagct catcggcggg ttgcaccagt cgatgttcta
     1801 cgtcggtgcg cgcaccgtgc ccgagctgaa ggcgcgcggc cgcttcatcc gcatcacgcc
     1861 cgctgggctg cgcgagtcgc acccccacga catcaccggc atcgtcgagg cgccgaacta
     1921 ctcccagggc tgacgtcagt cgccgatgat ggcgtcgcgg taggacgcca gggcgagccc
     1981 gccctcggcc gtgcggtcca ccgacgtcgc cgcgagcacc cctcgcacga tggcccgggc
     2041 cacgcagtcg gcggcggcgg tctgcaggtc cacctcgtcg aggccgctca cgcccggccg
     2101 ggcgccggtg gccagggcga acacggtgtc gccgtcgaac gccgtgtgca ccggtgacag
     2161 cgcccgcgcg aacccgtcgt gggcgacgcc ggccagcttg ctgcaccccg ccttggagag
     2221 ggtggcatcg gtcgcgacca cggccagcgt ggtcgccgtg ccggcccgca acgccgcgac
     2281 cgcagcggcg cgtgcctccc ggtacgaggt gaccagcccg gcatccggcg tcggcaggtc
     2341 gagatccgcc gccaggtggg cggccagcat ccgaccatcg ggcccgaacg gttcgccgac
     2401 ggcgttgacg acgacgagtg cgcccaccgt cacccccgag gacaggacgg cggatgccgt
     2461 ccccacgcca ccgcgcagac ctccggttcg cgcgccggta cccgccccca ccgagccctg
     2521 ctcgaccggg cccgcaccgg cagcggcgaa ggcggccgcg ccgtcgcccg gcctcgggtg
     2581 gttcagccac gtcccgccgc gcccgaggtc gaacaggatc gccgcaggca cgatcggcac
     2641 ccgctcgtgg ctgcccgggc ccaccggcca cccgcgaccg gcggcgtaga gccctgtcga
     2701 gacgccgtct gccgcagcga gcccgaacgc gctcccaccg gcgagcacca cggcatccac
     2761 ggcgtcgacc agggtgcggg ggtcgagcag gtcggtctcg cgggtccccg gcccgccgcc
     2821 gcgcacatcg accccgccga cggtgccggg cggagcgacg acgaccgtga cgccggtcag
     2881 ccagcccggc tcctcccggg tgcagtggcc gacgagcacg ccgtcgatgt cggtgatggc
     2941 gtcgtcgggt ccgggctgca cctgccccaa tatgccggat gccggccgcc cgaggtgggc
     3001 ggc
//
LOCUS       DAOSOC010000244         2973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_33239_length_2973_cov_6.154901, whole genome shotgun sequence.
ACCESSION   DAOSOC010000244 DAOSOC010000000
VERSION     DAOSOC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33239_length_2973_cov_6.154901"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            684..>2973
                     /locus_tag="PL014_12505"
     CDS             684..>2973
                     /locus_tag="PL014_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOT57957.1"
                     /translation="MRRAIDQGWTVRATAGPVPEHLASALTGVAAVAASVPGVVHLDL
                     LAAGLIPDPHLDLNESLLTWIGLVDWTYETTLPMTAAELAAAERHEILFDGLDTVATV
                     LLNGQVIAEVANQHRTHRLDVTGILTEGDNLLEVRFRSPVKYANAQSLTYGARPGPYP
                     MPFEAIRKSACSFGWDWGPATFTSGIWRPVHLVSWSGARFDDVLVRADAVGDGGRVEV
                     VARVASTSDAARTVTVSVAGVSGSATTAAGEREVTASVECATVDRWWPTGHGDQPLYD
                     IEVELACDGAVLDRVRRRVGFRTLHWDTTPDAAGTPFQLVVNDRPILVKGVNWIPDDS
                     FPVRVDRARYAQRLTQARAANVNLVRVWGGGIYESDSFYDLCDELGLLTWQDFLFACA
                     AYPEEEPLRSEVEAEARQNVARLAHHASLALLTGNNENLWGYEDWGWKERLDGRTWGA
                     HYYDELLPAVVAEVAPHVPYAPGSPFSPGGQHPNDPSHGTTHLWEQWNSLDWATYRDI
                     APRFVAEFGWQGPPTWSTLTTAISDDPLTPESPGMLAHQKAVDGNAKLVGGLVRHHRV
                     PDDMATWHWAMQLNQANAITCALDWFRSLAPSCSGAVVWQLNDCWPVTSWAAIDGSGR
                     EKPLYFALQNAFAPRVVSVQPRGGRLVASLGNDTDVPWEGSLTCRRLRFDGTVCGSKD
                     VAVSVPARGSVTIDLPAVVADADDPASEVVVVEALGTRGLWFFAEPRDSALAPPAVEV
                     SVAATDAGFDVSVSARTLVRDLT"
BASE COUNT          421 a         1044 c         1048 g          460 t
ORIGIN      
        1 cgacgcgggc gatgatgtcg ctgaggtggt tgcgtacctc gtcaagcggt tcgacggcca
       61 tagagaccag tgtggcgtcc gcggcggtcc gccctgcggc cagaagccag gaggtccgtt
      121 tgggcacaac accttgggcc tgcctgacag gccccgtatc gtcgacggag gagccgagcg
      181 gggcgcacga aagttcctac tgcgttccca gaaggcccgg acgctgtcgc acgacacagg
      241 tgtacataga tggacatcac tgtcgcacgg ctgtcgcacg aggccacttc cgacgcctgg
      301 atgccgcatc gtcgcaggtc aggcaggggt aggaacgaca tcccacactc cttgtaaaca
      361 gggggttgag ggttcgagtc ccttcgccag ctctcgggga tggtcagatg ccgccgggtg
      421 ctgttggggg gtcaagggcg cggcccaccg cgcgcttggc ctcccggctg tgatcggcgg
      481 ggccatcgac gtcatcgagg gtgagccggg tctgctcggg catccgtcgt cactgcacgc
      541 agcgctgggg ctgtctgtgc cgcatggctg gcaccagccg ggggtagcgt cgacaccatg
      601 acgtccgtcc cagacggcat cgctcgctac cgcgcggccg atgacctcga caccggcgcc
      661 tcggtctgat gggcgtgggc gttgtgaggc gggcgatcga ccaggggtgg acggtgcgcg
      721 cgaccgccgg gccggtaccc gagcacctcg cgtccgcgct cacgggcgtt gcagcggtgg
      781 cggccagcgt gcccggcgtc gtccacctcg acctgctggc agccgggctg atccccgacc
      841 cccacctcga cctcaacgag tcgctgctca cctggatcgg tctcgtcgac tggacgtacg
      901 agaccaccct gcccatgact gccgctgagc tcgccgcggc cgagcgccac gagattctct
      961 tcgacgggct cgacaccgtc gccacggtgc tcctgaacgg ccaggtcatc gccgaggtcg
     1021 cgaaccagca ccgcacccac cgactcgacg tcaccggcat cctcaccgag ggtgacaacc
     1081 tgctcgaggt gcgctttcgc agcccggtga agtacgcgaa cgcccagagc ctgacgtacg
     1141 gtgcgcgccc cggtccgtac ccgatgccct tcgaggccat ccgcaagtcg gcgtgcagct
     1201 tcggctggga ctgggggcct gcgacgttca cgagcggcat ctggcggccc gtgcacctgg
     1261 tgtcgtggag cggggcgcgg ttcgacgacg tcctcgtgcg cgcggacgcc gtgggcgacg
     1321 gtggtcgcgt cgaggtcgtc gcccgcgtgg cgtccacttc ggatgccgct cgcacggtga
     1381 ccgtgtccgt tgccggggtg agtggctcgg cgacgaccgc cgcaggcgag agggaggtca
     1441 cggcatccgt cgagtgcgcg accgtcgacc gctggtggcc gaccgggcac ggcgaccagc
     1501 ccctctacga catcgaggtc gaactcgcct gcgacggtgc ggtgctcgac cgcgtcaggc
     1561 gccgggtggg ctttcgcaca ctgcactggg acaccacgcc ggatgccgcg ggcaccccct
     1621 tccagctcgt cgtcaacgac cggccgatcc tcgtcaaggg cgtcaactgg atccccgacg
     1681 actccttccc ggtgcgggtg gaccgagccc gctacgccca gcgcctcacc caggccaggg
     1741 ccgcgaacgt caacctcgtg cgggtctggg gcggcggcat ctacgagtcc gacagcttct
     1801 acgacctgtg tgacgagctg ggcctgctga cctggcagga cttcctcttc gcctgcgcgg
     1861 cctacccgga ggaggagccc ctgcggtccg aggtcgaggc cgaggcccgg cagaacgtgg
     1921 cccggctcgc gcaccacgcc tcgttggcgc tgctcaccgg taacaacgag aacctgtggg
     1981 gctacgagga ctgggggtgg aaggagcggc tcgacggccg cacctggggt gcgcactact
     2041 acgacgagct gctcccggcc gtcgtcgccg aggtggcgcc gcacgtgccg tacgccccgg
     2101 gcagcccgtt cagcccgggc gggcagcacc cgaatgaccc gagccacggc accacccacc
     2161 tgtgggagca gtggaactcc ctggactggg cgacctaccg ggacatcgcc ccgcggttcg
     2221 tcgcggagtt cggctggcag gggccgccga cctggtcgac cctcacgacg gcgatcagcg
     2281 acgaccccct cacccccgag tcgcccggca tgctcgccca ccagaaggcc gtcgacggca
     2341 acgccaagct cgtgggtggg ctcgtgcgcc accaccgggt gcccgacgac atggcgacct
     2401 ggcactgggc gatgcagttg aaccaggcca acgccatcac gtgcgccctc gactggttcc
     2461 gctccttggc accttcgtgt tcgggtgccg tcgtctggca gctcaacgac tgctggccgg
     2521 tgacgtcgtg ggcggccatc gacggcagcg gccgcgagaa gcccctctac ttcgccctgc
     2581 agaacgcgtt cgccccacga gtggtgtcgg tccagccccg gggtggccga ctggtggcct
     2641 cgctcggcaa cgacacggac gtgccgtggg agggctcgct cacgtgtcgg cggttgcggt
     2701 tcgacggcac ggtgtgtggg tcgaaggacg ttgcggtgtc ggtgcccgcc cgtgggtcgg
     2761 tgaccatcga cctccccgcg gtggtcgcgg acgccgacga cccggcatcc gaggtcgtgg
     2821 tcgtcgaggc cctcgggacg cgaggcctct ggttcttcgc cgagcctcgg gacagtgcgc
     2881 tcgctccccc agccgtcgag gtgagcgtgg cggcgacgga tgccgggttt gacgtctccg
     2941 tgtccgcacg gacgctcgtg cgtgacctga ccc
//
LOCUS       DAOSOC010000245         2961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_33425_length_2961_cov_9.777357, whole genome shotgun sequence.
ACCESSION   DAOSOC010000245 DAOSOC010000000
VERSION     DAOSOC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33425_length_2961_cov_9.777357"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PL014_12510"
     CDS             <1..1379
                     /locus_tag="PL014_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOT57958.1"
                     /translation="GEPGERITSRSADIGMDWQATAYPVEFYALGGQGTGIPVRASVS
                     TFGPTLLSKVMGLNATQESSLGLVFHYADTKGLWLDTIPDLRAVVQFLTSPEGKADLK
                     DLGGLSSVTAGVILRELVNFSAQGADVFFGLPEFDTADLLRTAPDGRGLISMLELPGV
                     QDRPQLFSTFLMWLLADLFHDLPEVGDLDKPKLVFFFDEAHLLFNGASKQFLDAIQQT
                     VRLIRSKGVGVFFVTQSPKDVPGDVLAQLGSRVQHALRAFTPDDAKALKAAVRTYPNT
                     DYDLEELLTSLGTGEAVVTVLSDKGVPTPVAWTRMRAPESLMAPSPPALLTEAVTVSP
                     LAAKYSTYLERESASEMLGKAEIARTEAEKLAAQEAENAKVRAAAAAAAEKERLAAEK
                     VAAREAAKGAAEQEKARAAEERRKAAEPGVVDKVVKSSAFKSMLRSAGTVIGREITRS
                     VFGTSRRR"
     gene            complement(1569..1961)
                     /locus_tag="PL014_12515"
     CDS             complement(1569..1961)
                     /locus_tag="PL014_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57959.1"
                     /translation="MITVELARDLIGAGVLWEPAAGDRFVIDAELLTGEVFWISDLTV
                     EVQTYRDQSLLGFNGTTEWALDSVTLDQALWLPREDQLRELLGDRLEVVRRLEDEWEL
                     TYAVAPGYVRTVTDADLECAYAKAILTL"
     gene            complement(2050..2961)
                     /locus_tag="PL014_12520"
     CDS             complement(2050..2961)
                     /locus_tag="PL014_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOT57960.1"
                     /translation="MGFATIVAVIGVGALVAHLGVVDLGAQQVLSRIAFFVASPALLV
                     TTVAQADVSDVLSRGLAATVVAVVVPAGTYMVIARWRWRRSAGEQVIGALSSSYVNAG
                     NLGLPVAAYVLGDAALVAPTLLLQLLVLQPLALAVLDADVRGTRPSVSEVLLRPFTNP
                     LTIGTLVGLLLSITGWRLPPLVADPVELIGAMAVPGMLLAYGISLRLGPGFGGDVPPG
                     ELALTSALKLVVQPLVAYAVAHHALGLSGHALLAVVVCASLPTAQNIFVHATRYDRSS
                     TLARDTILVTTTGAVPVIMLVVWALGG"
BASE COUNT          478 a         1050 c         1032 g          401 t
ORIGIN      
        1 cgggtgaacc gggggagcgg atcacctcac ggtcggccga catcggcatg gactggcagg
       61 cgacggccta cccggtggag ttctacgccc tcggtgggca gggcaccggt atccccgtgc
      121 gggccagcgt ctcgacgttc gggccgaccc tgctctcgaa ggtcatgggc ctgaacgcca
      181 cccaggagtc cagcctgggt ctggtcttcc actacgccga caccaagggg ttgtggctcg
      241 acaccatccc cgacctgcgg gccgtcgtcc agttcctcac cagccccgag ggcaaggccg
      301 acctcaagga cctcggtggc ctgtcgtcgg tcacggccgg ggtgatcctg cgcgaactgg
      361 tgaacttctc cgcccagggc gccgacgtgt tcttcggcct gcccgagttc gacaccgcag
      421 acctgctgcg caccgcacct gacggccgcg gcctgatctc catgctcgag ctgccggggg
      481 tgcaggaccg cccgcagctc ttctcgacct tcctcatgtg gctgctcgcc gacctgttcc
      541 acgacctgcc cgaggtcggg gacctcgaca agccgaagct cgtcttcttc ttcgacgagg
      601 cgcacctgct cttcaacggg gcgagcaagc agttcctcga cgcgatccag cagaccgtac
      661 ggctcatccg gtccaagggg gtcggtgtgt tcttcgtgac ccagtcaccc aaggacgtgc
      721 cgggcgacgt gctggcccag ctcggcagcc gcgtccagca cgccctgcgc gcgttcaccc
      781 cggacgacgc gaaggccttg aaggcggcgg tgcgcacgta cccgaacacc gactacgacc
      841 tcgaggagct cctgacctcg ctcggcaccg gtgaggccgt ggtgaccgtc ctctcggaca
      901 agggagtgcc gacgccggtc gcctggaccc ggatgcgcgc cccggagtcg ctcatggccc
      961 cgtcgccgcc ggcgctgctc accgaggccg tcaccgtgtc gccgctggcc gccaagtact
     1021 cgacctacct cgagcgcgag tcggcctcgg agatgctcgg caaggctgag atcgcccgta
     1081 ccgaggccga gaagctcgct gcgcaggagg ccgagaacgc caaggtgcgc gccgcagccg
     1141 ctgctgcagc cgagaaggag cggctggcgg cagagaaggt cgccgcgcgc gaggccgcca
     1201 agggggctgc tgaacaggag aaggcccgcg ccgccgagga gcgccgcaag gctgctgagc
     1261 cgggcgtggt cgacaaggtc gtgaagtcga gtgcgttcaa gtcgatgctg cgctcggcgg
     1321 gcacggtcat cggccgagag atcacccggt cggtgttcgg gaccagccgc cgccgctgac
     1381 cccgtcatac cccgcggagt cagggctgct tgtcgcggtg ccgctgatcc gctcgtcgca
     1441 ggtccttcct ccggtcgcag atccgtactc cggttgtgcg tccttgtagc agcgcccatc
     1501 cggagcaagg acgcacatcg ggagcaagga tctgcgacga gcagtgcgca acccccgctg
     1561 cggccgggtc agagggtcag gatggccttg gcgtacgcgc actcgaggtc ggcgtcggtg
     1621 acggtgcgca cgtaccccgg ggcgacggca tacgtcagtt cccactcgtc ctcgagccgc
     1681 cgcaccacct cgaggcggtc accgagcagc tcgcgcagct ggtcctcgcg gggtagccac
     1741 agcgcctggt cgagcgtcac cgagtccagc gcccactcgg tggtgccgtt gaagccgagc
     1801 agtgactggt cgcggtaggt ctgcacctcg acggtgaggt cgctgatcca gaacacctca
     1861 ccggtcagca gctcggcatc gatgacgaac cggtcgccgg cggcgggctc ccacaggacg
     1921 ccggcgccga tgaggtcacg cgcgagctcg acggtgatca tggcgccatt gtggaccacg
     1981 accggatgcc ggcccacgag cgatcgtgac ccatgaccgg atgccggcca cccggcatcc
     2041 gctcgcacct cacccgccga gcgcccagac cacgagcatg atcacgggca ccgccccggt
     2101 cgtcgtcacg aggatggtgt cgcgcgccaa ggtgctcgac cggtcgtagc gggtggcgtg
     2161 cacgaagatg ttctgcgccg tcggcaggct cgcgcacacg acgacggcga gcagggcgtg
     2221 acccgacagc ccgagcgcat ggtgcgccac ggcatacgcc acgaggggct ggacgacgag
     2281 cttgagcgcg ctcgtcaggg caagctcacc cggggggacg tcgcccccga accccggtcc
     2341 caggcgcagc gagatgccgt aggcgagcag catgccgggc actgccatgg ccccgatcag
     2401 ctcgaccgga tcggccacga gcggcggcag gcgccagccc gtgatcgaca ggaggagccc
     2461 cacgagggtg ccgatcgtca gggggttcgt gaacggccgc agcaacacct cggagactga
     2521 cggtcgggtg ccgcgcacgt cggcgtcgag gacggccagg gccaggggct ggagcacgag
     2581 cagttgcagc agcagggtcg gcgcgaccag tgcggcgtct ccgagcacgt aggcggccac
     2641 gggcaggccg aggttgccgg cgttgacgta ggagctcgag agggccccga tgacctgctc
     2701 gcccgccgat cggcgccacc gccaccgcgc gatcaccatg taggtgcctg ccggcacgac
     2761 gaccgcgacc accgtcgcgg ccagcccgcg cgacaacacg tcgctgacat ccgcctgggc
     2821 gacggtcgtg acgagcagcg cgggggacgc cacgaagaac gcgatccgac tgagcacctg
     2881 ctgcgccccg aggtcgacga ccccgaggtg ggcgaccagc gcgccgacac cgatgaccgc
     2941 gacgatcgtc gcgaacccca g
//
LOCUS       DAOSOC010000246         2918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_34047_length_2918_cov_11.120154, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000246 DAOSOC010000000
VERSION     DAOSOC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34047_length_2918_cov_11.120154"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PL014_12525"
     CDS             complement(<1..1280)
                     /locus_tag="PL014_12525"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT57961.1"
                     /translation="MTPEQLSAAIRAALQSAVDAGDLAITVPAEVRVERPRNRDHGDW
                     STNIALQLAKGAGVAPRELATALATHLASVTGVQAVDVAGPGFLNITLEAASAGELAR
                     AIVEAGPTFGHGSTESGRVVNLEFISANPTGPLHIGHTRWAALGDAMRRLLVAAGAQV
                     AAEYYINDAGAQMDKFGASVLARAKGQEPPEDGYPGAYVADRAAEVLAQRPDLLELDE
                     ADAAVLARDLGYVAQLAAIQRTLDGFGVHFDVWFSERGLHDGGAVAQAVARLREQGHV
                     FDHDGAVWLRTTDFGDDKDRVLIRADGTPTYFAADAAYYLSKKDRGFDEKIYLLGADH
                     HGYINRLKAIAACAGDDPEHNIEVKIGQLVNIAGERMGKRLGNALYMDDLIAWIGSDA
                     VRYSLARYPADSPLSLDGEEMRRQTNDNPVFYVQ"
     gene            complement(1291..1719)
                     /locus_tag="PL014_12530"
     CDS             complement(1291..1719)
                     /locus_tag="PL014_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT57962.1"
                     /translation="MQSGRGAEHEAPLVLVCDDTEPIRRLLRINLELAGFRVAEATDG
                     HAAMARLIDPEAQRPAVIVLDSQMAPYDGWWAIAAIRSHPRLDPVPVVLLTAAVIAHD
                     APQASDAGFDAFVAKPFDPDELITAVSRLAERGRGTQSRR"
     gene            1793..1864
                     /locus_tag="PL014_12535"
     tRNA            1793..1864
                     /locus_tag="PL014_12535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1826..1828,aa:Arg,seq:ccg)
     gene            complement(1938..>2918)
                     /locus_tag="PL014_12540"
     CDS             complement(1938..>2918)
                     /locus_tag="PL014_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT57963.1"
                     /translation="FVGAEFAAMAARRSRLEPLVAAGNNRARIVLEATEQMGSLLACA
                     QLGITICSVLIGAISEAALHHALVPIVERAGLSPRVTDILALVLALLVVIYLHVVVGE
                     MIPKNLAIAGPERSALVLVPPLQLMTRALRWPIMAMERIAKAIVRMCGVEPKDEVASE
                     FTVEEVAHILQASHEEGLVLSEQFGRLDAAIEFSDKDASDVGVRLADLVTVGLDTTPD
                     DIERLVAKRGFSRYPVAGADGYLIGYLHLKDVLFADEEERLQPVPAKRIRRMANVRRT
                     DEVESVLRTMQQTGSHLARVVADDGEVVGVVFLEDVIEQLVGEVADASQR"
BASE COUNT          437 a          995 c         1052 g          434 t
ORIGIN      
        1 tactgcacgt agaagaccgg gttgtcgtta gtctgccggc gcatctcctc accgtccagc
       61 gacagcgggg agtcggcggg gtagcgcgcg agcgagtagc gcaccgcatc cgatccgatc
      121 cacgcgatga ggtcgtccat gtagagcgca ttgccgagcc gcttgcccat gcgctccccg
      181 gcgatgttca cgagctgacc gatcttgacc tcgatgttgt gctcggggtc gtcgccggcg
      241 caggccgcga tggccttgag tcggttgatg tagccgtggt ggtctgcgcc caggaggtag
      301 atcttctcgt cgaagccgcg gtccttcttg ctcaggtagt acgcggcgtc ggcggcgaag
      361 tacgtgggtg tgccgtcggc ccggatgagg acacggtcct tgtcgtcgcc gaagtcggtc
      421 gtgcgcagcc agaccgcgcc gtcgtggtcg aacacgtggc cctgttcgcg caggcgggcc
      481 acggcctgcg cgaccgcgcc cccgtcgtgc agacctcgct cggagaacca cacgtcgaag
      541 tgcaccccga acccgtcgag ggtgcgctgg atggcggcca gctgagcgac gtaaccgagg
      601 tcgcgagcca gcaccgcggc atccgcctcg tcgagctcga gcaggtcggg ccgctgcgcg
      661 aggacctcgg cggcccggtc ggcgacgtag gcacccgggt agccgtcctc ggggggctcc
      721 tgacccttcg cgcgggccag gaccgaggca ccgaacttgt ccatctgcgc cccggcatcg
      781 ttgatgtagt actcagccgc gacctgggcc ccggccgcga cgagaaggcg gcgcatcgcg
      841 tcaccgaggg ccgcccagcg ggtgtgcccg atgtgcaggg gccccgtcgg gttggccgag
      901 atgaactcca ggttgaccac acggcccgac tcggtgctgc cgtggccgaa cgtcgggccc
      961 gcctcgacga tggcacgcgc cagctcaccg gcgctcgcgg cctcgagagt gatgttgagg
     1021 aagcctgggc ccgcgacgtc gacggcctgc acccccgtca cggacgcgag gtgcgtcgcg
     1081 agcgccgtcg cgagctcgcg cggggcgacg ccggcgccct tggcgagctg aagggcgatg
     1141 ttggtcgacc agtccccgtg atcgcggttg cgcggtcgct cgaccctgac ctcggcgggc
     1201 acggtgatcg cgaggtcacc tgcgtcgacg gcggactgga gggcagcgcg gatcgcggcc
     1261 gagagctgtt cgggggtcac ccgcccgatt ctatcgacgc gactgggtgc ccctgccgcg
     1321 ctccgcgagc cgtgagacgg cggtgatgag ttcgtccggg tcgaagggtt tggcgacgaa
     1381 ggcatcgaaa ccggcgtcgc tcgcctgggg tgcgtcgtgc gcgatgacgg cagccgtgag
     1441 caggacgacc ggcacggggt ccagtcgcgg gtgggaacgg atcgcggcga tcgcccacca
     1501 cccgtcgtag ggcgccatct gggagtccag gacgatgacg gccgggcgct gcgcctccgg
     1561 gtcgatcaga cgcgccatcg cggcatgacc gtcggtggcc tccgcgaccc ggaagccagc
     1621 caactcgagg ttgatgcgga gcagacgacg gatcggctcg gtgtcgtcac acaccagcac
     1681 caacggcgct tcgtgctcgg ctcccctgcc ggattgcatg tggcccaccc taggctggta
     1741 gcctcaccgg gcgctgggta actcctcgga gatccggcgc gggccatcac cggcccccgt
     1801 agctcagggg atagagcacc gccctccgga ggcgggagcg caggttcgaa tcctgccggg
     1861 ggcacgcagt tccgaggcca cgacagtccc tgtcgtggcc tcgccgctgg acgggcccgc
     1921 gcggggcgga gtccgggtca gcgctgactg gcgtccgcga cctcgccgac gagctgctcg
     1981 atgacgtcct cgaggaagac gacaccgacc acttccccgt cgtcggcgac cactcgcgcc
     2041 aggtgtgatc cggtctgctg catcgtccgc aacacgctct cgacctcgtc ggtccggcga
     2101 acgttggcca tccgccggat gcgcttggcc gggaccggct gcagccgctc ctcctcgtcg
     2161 gcgaagagga cgtccttgag gtgcagatag ccgatgaggt acccgtcggc gccggccacc
     2221 gggtagcggg agaagccgcg cttggccaca aggcgctcga tgtcgtccgg ggtcgtgtcc
     2281 agacccacgg tcaccaggtc ggccaggcgg acaccgacgt cggaggcgtc cttgtcggag
     2341 aactcgatcg cggcgtcgag ccggccgaac tgctcggaaa ggaccagccc ctcctcgtgc
     2401 gacgcctgga ggatgtgcgc gacctcctcg acggtgaact cggaggcaac ctcgtccttg
     2461 ggctcgacac cgcacatccg gacgatggcc ttcgcgatgc gctccatcgc catgatcggc
     2521 caccgaagcg cccgcgtcat gagttgcagc ggaggcacga gcaccagggc ggagcgctca
     2581 gggccggcga tggcgaggtt cttcgggatc atttcgccca cgacgacgtg caggtagatg
     2641 acgaccagca gggccaggac gagcgccagg atgtccgtga cgcgcgggct cagccccgct
     2701 cgctcgacga tgggcacgag ggcgtgatgg agcgcggcct cggagatggc accgatgagc
     2761 accgagcaga tcgtgatgcc gagctgggcg caggccagca gcgagcccat ctgctcggtc
     2821 gcctccagga cgatccgcgc ccggttgttg cccgcggcga ccaacggttc gaggcgactg
     2881 cgccgcgctg ccatcgcggc gaactcggcg cccacgaa
//
LOCUS       DAOSOC010000247         2916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_34073_length_2916_cov_9.398113, whole genome shotgun sequence.
ACCESSION   DAOSOC010000247 DAOSOC010000000
VERSION     DAOSOC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34073_length_2916_cov_9.398113"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /gene="ndk"
                     /locus_tag="PL014_12545"
     CDS             complement(<1..416)
                     /gene="ndk"
                     /locus_tag="PL014_12545"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOT57964.1"
                     /translation="MTTERSLVLVKPDGYARGLTGEVLRRIEAKGYRLVALQVVTPDL
                     ERLHAHYAEHEGKPFFEPLVEFMSSGPVTAVVIEGEQCIAGFRALAGATDPTAALPGT
                     IRGDLGRDWGAKVQKNIVHGSDSPESAAREIGIWFP"
     gene            complement(437..850)
                     /locus_tag="PL014_12550"
     CDS             complement(437..850)
                     /locus_tag="PL014_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HOT57965.1"
                     /translation="MTRLPLYGRQGKFTFRLLAITLFGQSIILSFFALVARGNAIAAG
                     RPEEGERLLWIGLGLALLALVASGLMRRPYGITLGWIVQALTWASAVVVTAMIGVALV
                     FTALWLLLLVQGMKVDRIVAEREQSEAGEPAPDAA"
     gene            complement(847..1116)
                     /locus_tag="PL014_12555"
     CDS             complement(847..1116)
                     /locus_tag="PL014_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57966.1"
                     /translation="MSPLLKLLLIVAVAVLVWWVVTSVRASRGSTGTTFTPVDELAPE
                     VQQTIDAALSRGELVAAIKHYRAATGAGLAQSKAAVETRRWKTGA"
     gene            complement(1147..2541)
                     /locus_tag="PL014_12560"
     CDS             complement(1147..2541)
                     /locus_tag="PL014_12560"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOT57967.1"
                     /translation="MSPAPDAVQRDAAHQLDLTRRMREVEASILSRAPEHDLEPSLDR
                     IRAVMELLGDPQRTFPVIHVTGTNGKTSTSRIIEALLRELGLNTGRFTSPHLHSMTER
                     IAVGGQPIDAERFLAAYDEVIPFVEMVDARSIIDGGPAMTYFEVIVAVAYAAFADLPV
                     DVAVMEVGMGGAWDATNVIDAPVSVITPIGLDHQHFLGDTIELIATEKAGIIKPDAIT
                     VMAVQPEPDAVDVLGARAAEVGARLVAEGVDIGVTARDVAFGGQQISVRGLAAEYEDL
                     FLPLHGTHQAQNAAVAIAAVEAFLGGGEQPLDIEVLRAGLATAESPGRLEIVRRSPTV
                     LVDAAHNPAGAEVLARAITESFTFARLVGVIAVMKDKDALGVLQALEPVLDDVVVTRT
                     TSPRAMTPRQLGELAAEVFGEQRVTVVEELPDALDVAAGMADADGGVSGGVLATGSVV
                     TAAEVRMLLGTTAV"
BASE COUNT          434 a         1025 c         1056 g          401 t
ORIGIN      
        1 tcggggaacc agatgccgat ctcgcgcgcg gccgactcgg gcgagtccga gccgtggacg
       61 atgttcttct gcaccttggc gccccagtcg cgcccgaggt caccgcggat cgtgccggga
      121 agggcggccg tggggtcggt ggcaccggcc agggcccgga agccggcgat gcactgctca
      181 ccctcgatga cgacggcggt caccggcccg gaggacatga actcgacgag cggctcgaag
      241 aacggcttgc cctcgtgctc ggcgtagtgg gcgtgcagcc gctcgaggtc gggggtcacg
      301 acctggaggg cgacgaggcg gtaccccttg gcctcgatgc ggcgcagcac ctcaccggtg
      361 aggccgcggg cgtagccgtc gggcttgaca aggacgaggg agcgttcggt ggtcacgcac
      421 ccaccctagt cggccctcag gccgcgtcgg gtgcgggttc gccggcttct gactgctcac
      481 gctcggcgac gatccggtcg accttcatcc cctgcacgag cagcagcagc cacaacgccg
      541 tgaagacgag cgcgaccccg atcatcgccg tgacgaccac ggcactggcc caggtgaggg
      601 cctgcacgat ccagccgagg gtgatgccgt aggggcggcg catcaggccc gatgcgacga
      661 gggcgagcag ggccagcccc agcccgatcc acaggaggcg ctccccctcc tccgggcgcc
      721 cggcggcgat cgcgttgccg cgggcgacca gggcgaagaa cgacaggatg atcgactgtc
      781 cgaacagggt gatcgcgagc agccggaagg tgaacttgcc ctgccggccg tagagcggca
      841 gacgggtcat gcgccggtct tccagcgccg ggtctccacg gcggccttgg actgggccag
      901 cccggccccg gtcgccgccc ggtagtgctt gatcgccgcg accagctcac cgcgcgacag
      961 cgccgcgtcg atcgtctgct gcacctcagg agcgagctcg tcgaccgggg tgaacgtcgt
     1021 cccggtgctg ccgcggctgg cgcgcaccga ggtcacgacc caccagacca gcaccgcgac
     1081 agcgacgatg agcagcagct tgaggagcgg ggacatgtcc ccaagggtaa gcgccccgcc
     1141 gtgcggtcag acggcggtgg tgccgagcag catccgcacc tcggccgcgg tgacgaccga
     1201 cccggtggcg agcactcccc cgctgacgcc gccatcggca tccgccatcc cggccgcgac
     1261 gtccagcgcg tccggcagct cctcgacgac cgtgacccgc tgctccccga acacctcggc
     1321 cgcgagctct ccgagctggc ggggcgtcat cgcccgtggc gaggtcgtgc gggtgacgac
     1381 gacgtcgtcg agcaccggct cgagcgcctg gagcaccccg agggcgtcct tgtccttcat
     1441 cacggcgatg accccgacga ggcgggcgaa ggtgaacgac tcggtgatgg ccctggccag
     1501 cacctcggca cccgcagggt tgtgcgcggc gtcgacgagc accgtcgggc tgcgccgcac
     1561 gatctccagg cgccccgggg actcagcggt cgccaacccc gcccgcagca cctcgatgtc
     1621 gagcggctgc tcacccccgc cgaggaaggc ctcgaccgcc gcgatggcaa ccgccgcgtt
     1681 ctgcgcctga tgggtgccgt gcagcggcag gaacaggtcc tcgtactccg ccgcgagccc
     1741 gcgcaccgag atctgctggc caccgaaggc gacgtcgcgc gcggtgacgc cgatgtcgac
     1801 cccctcggcg acgagccggg caccgacctc ggcggcgcgc gcaccgagca cgtcgacggc
     1861 atccggctcg ggttgaacgg ccatgaccgt gatcgcgtcg ggcttgatga tcccggcctt
     1921 ctcggtcgcg atgagctcga tggtgtcgcc gaggaagtgc tggtggtcca gcccgatggg
     1981 cgtgatgacg ctgaccggcg cgtcgatgac gttcgtcgcg tcccaggcgc cacccatgcc
     2041 gacctccatg accgcgacgt cgaccggcag gtccgcgaac gcggcgtagg cgacggccac
     2101 gatgacctcg aagtacgtca tcgccggacc gccgtcgatg atcgagcggg cgtcgaccat
     2161 ctcgacgaac gggatcacct cgtcgtaggc cgcgaggaac cgctcggcgt cgatgggctg
     2221 cccaccgacc gcgatgcgct cggtcatcga gtgcaggtgg ggcgaggtga agcgcccggt
     2281 gttcaggccg agctcgcgca gcagggcctc gatgatccgc gacgtgctcg tcttgccgtt
     2341 ggtccccgtg acgtggatga cggggaaggt gcgctgcggg tcgcccagga gctccatgac
     2401 ggcgcggatg cggtcgagcg agggctcgag gtcgtgctcg ggtgcccggg agaggatcga
     2461 ggcctcgacc tcgcgcatcc ggcgggtcag gtcgagctgg tgcgcggcat cgcgctggac
     2521 ggcatcgggg gcagggctca ccggcccatt gtcgctgact ccggtgcgca gcggtcacac
     2581 gacggaggtg gcccccaagc gacctctccc gtccagtcgg tgtccggcgt accggccccc
     2641 gtggttctcc acgacgacgg tgtacgtgcc gggctgggcc caaggaacag ggaactttca
     2701 ccgcatgccg gtcagcgcct cacgatccgc aggcgggcgg ggaagcgctc gcccacggtc
     2761 acctcggtga cgtccgcggt atcccggtcg agggcgtcgg ggtccggcgt caccgacagg
     2821 tggcaggcgg tcggcaggcg acggtgcacg cacgcgtagc ggtgccctaa ctgaccagac
     2881 tctcgatgcg gttcaggtca tcctcgtggg tgtcgt
//
LOCUS       DAOSOC010000248         2872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_34777_length_2872_cov_6.365992, whole genome shotgun sequence.
ACCESSION   DAOSOC010000248 DAOSOC010000000
VERSION     DAOSOC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34777_length_2872_cov_6.365992"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..470)
                     /locus_tag="PL014_12565"
     CDS             complement(54..470)
                     /locus_tag="PL014_12565"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOT57968.1"
                     /translation="MRRSTHISRSLAVTALLGAGLVLGGCGSESVDLAGRSFTSTEVR
                     GHDLVEGSKVALSFEDGRISANAGCNTMNGAATWDGDTLQVAGPLAMTMMACDEALMD
                     QDQWLSEFLTSSPALAVDGETLTLGDDTTGITLSEN"
     gene            623..1510
                     /locus_tag="PL014_12570"
     CDS             623..1510
                     /locus_tag="PL014_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010080265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HOT57969.1"
                     /translation="MNTDQLDRIRTARGFIAALDQSGGSTPKALRLYGIDDTAYATEA
                     EMFDLVHAMRTRIITSPVFTGERILAAILFEMTMDRQILGKGAPEYLWQDKHVVPILK
                     VDKGLADETDGVRLMKPMPDLDAVLARAIGHGVFGTKMRSVISWANERGVAAVVRQQF
                     EVGRRILAAGLVPIIEPEIDIASPQKAAAEALLHAALVDEIGALDADQQIMLKLTLPE
                     EDDLYADLVAHPNVLRVAALSGGYTRDEATERLARNHGVIASFSRALTQGLRAQQGQA
                     DFDAALDASIESIYRASIT"
     gene            1529..2305
                     /locus_tag="PL014_12575"
     CDS             1529..2305
                     /locus_tag="PL014_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT57970.1"
                     /translation="MGTAEVPHVVAVLGRGVVDADEPVVTADDLGLTRGDGCFDSARV
                     VTDAGGTGRVVDLDEHLARLASSAEALAIANPSRGEWRGLVAEALASWPTPGEAVLKL
                     VLTRGREWRDAGPTAIVSITHRGPSSPAAASARTITAVTLSRGHPSDAFADAPWLLGG
                     TKTLSYVVNVAAAREARRRGADDVIFTTTDDFALDGPTSGLLVARDGAFLSTPTGGTG
                     ILESITVATILEAARADGFASRYELIPVDDLYRADGVWLV"
     gene            complement(2494..>2872)
                     /locus_tag="PL014_12580"
     CDS             complement(2494..>2872)
                     /locus_tag="PL014_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57971.1"
                     /translation="ARTKPASSSPSRRRKCRPVIAKYSAAQVVGLALVVLMAVIWLAW
                     VIRRRAAFLRRLYVPTCVIGGFAMLALGPQVLGSLTGTQGLFPPEPVRPAGAFGPRPE
                     IVGRQPQVVQDRIGQAGRGKAHG"
BASE COUNT          423 a         1033 c         1019 g          397 t
ORIGIN      
        1 cactttggtg caatctaggg gcgtgtcagc ccctcaatcg tgaagggccg aggctagttc
       61 tcggacagcg tgatgccggt ggtgtcgtca cccagggtca gcgtctcgcc gtcgaccgcg
      121 agtgccggcg aggaggtgag gaactcgctg agccactggt cctggtccat gagtgcctcg
      181 tcgcaggcca tcatcgtcat cgccagcggc ccggcgacct ggagggtgtc gccgtcccag
      241 gtcgcggctc cgttcatggt gttgcacccg gcgttcgcgg agatgcggcc gtcctcgaag
      301 gacagcgcga ccttgctgcc ctcgacgagg tcgtggcccc gcacctcggt ggaggtgaac
      361 gagcgaccgg cgaggtcgac ggactcgctc ccgcacccgc cgagcaccag gccggcgccg
      421 aggagggccg tgacggcgag ggatcgggag atgtgggtgc tgcggcgcat gggtcctcct
      481 ggtcggtggg ccaccaccct atcgccggcc ccagtgatcc cgacacccca cacggttcgg
      541 gtgtgacggg tcgctccccc ggagcccttg acgcggccct gacggcggcg taggacggag
      601 gtgacccgag gaagtggcgc ccgtgaacac cgaccagctc gaccggatcc gcaccgcacg
      661 tggtttcatc gccgcgttgg accagagcgg cggcagcacg ccgaaggccc tgcggctcta
      721 cggcatcgac gacacggcgt acgccaccga agcagagatg ttcgacctcg tccacgcgat
      781 gcgcacccgc atcatcacca gcccggtgtt cacgggtgag cggatcctcg ccgcgatcct
      841 cttcgagatg acgatggacc gccagatcct cggcaagggg gcgccggagt acctgtggca
      901 ggacaagcac gtcgtgccga tcctcaaggt cgacaagggc ctggccgacg agaccgacgg
      961 ggtgcggctg atgaagccga tgcctgacct ggatgccgtg ctcgcccggg ccatcggcca
     1021 cggcgtgttc ggcacgaaga tgcgctcggt catctcgtgg gcgaacgagc gtggcgtcgc
     1081 cgccgtcgtg cgccagcagt tcgaggtcgg ccgacggatc ctggcggccg ggctcgtgcc
     1141 gatcatcgag cccgagatcg acatcgcgag cccgcagaag gcggcggccg aggccctgct
     1201 ccacgcggcc ctggtggacg agatcggtgc actggatgcc gaccagcaga tcatgctcaa
     1261 gctcaccctg cccgaggagg acgacctgta cgccgacctc gtggcgcacc cgaacgtgct
     1321 gcgcgtcgcc gccctctccg gtgggtacac gcgcgacgag gccaccgagc ggctggcccg
     1381 caaccacggc gtcatcgcga gcttctcccg ggccctgacg cagggcctca gggcccagca
     1441 gggccaggcc gacttcgacg ccgcgctcga cgcgtcgatc gagagcatct accgggcgtc
     1501 gatcacgtga cccgtgcggt gggacaccgt gggcacggct gaggtgccgc acgtcgtcgc
     1561 ggtgctcggc cgtggtgtcg tggacgccga cgagccggtg gtcaccgccg acgacctcgg
     1621 cctgacccgc ggcgacgggt gcttcgactc ggcgcgcgtc gtcacggatg ccgggggcac
     1681 cggtcgggtg gtcgacctcg acgagcacct ggcccgactc gcctcctcgg ccgaggcgct
     1741 ggccatcgcc aacccgtcgc gtggtgagtg gcgcggcctg gtcgccgaag ccctggcctc
     1801 gtggcccacg cccggcgagg ccgtgctcaa gctcgtcctg acccgcggcc gcgagtggcg
     1861 cgacgccggg cccacggcga tcgtgagcat cacccaccga ggtccctcgt cgcccgctgc
     1921 cgcgtcggcc aggacgatca cggcggtcac cctgagccgc ggccacccgt cggacgcctt
     1981 cgccgacgcg ccctggctac tcggcgggac caagacgctg tcatacgtcg tcaacgtcgc
     2041 cgcggcccgc gaggccaggc ggcgcggcgc cgacgacgtc atcttcacca cgaccgacga
     2101 tttcgccctg gatggcccga cgtcggggct gcttgtcgcc cgggacggcg ccttcctcag
     2161 cacgccgacg gggggaaccg gcatcctcga gtcgatcacc gtggcgacga tcctcgaggc
     2221 ggcccgggcc gacgggtttg cgtcgcggta cgagctgatc cccgtcgacg acctctaccg
     2281 cgcggacggc gtgtggctcg tgtagtcggg gcgtggcccg gtgctcgtca cctcgctcga
     2341 cggccggagc atccactccg acgagggact ggcagcccgg atcctgcgct acgccctctt
     2401 ctgacccgac ccctccggcc caaccgcggg cccgcgccgg ccccgggtgg ggcaccatgg
     2461 cgaccatgac cgagcagggg gctcacccga cacctacccg tgggccttgc cgcgaccggc
     2521 ctgtcctatg cggtcctgca ccacctgggg ctgccgcccg acgatctcgg gccgggggcc
     2581 aaaggcaccc gctgggcgga ctggctcggg cgggaacagg ccctgcgtgc cggtgagcga
     2641 cccgagcacc tgcggcccga gagccagcat ggcaaagccg ccgatgacgc acgtcgggac
     2701 gtagagcctg cgcaggaagg ccgcccggcg ccggatgacc caggccagcc agatgacggc
     2761 catgagcacg acgagcgcca gaccgacgac ctgggcggcg gagtacttcg cgatcaccgg
     2821 gcggcatttc ctacgtcgag atggcgaaga actggcgggc ttcgtccgag cg
//
LOCUS       DAOSOC010000249         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_35574_length_2822_cov_10.837008, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000249 DAOSOC010000000
VERSION     DAOSOC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35574_length_2822_cov_10.837008"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PL014_12585"
     CDS             complement(<1..358)
                     /locus_tag="PL014_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT57972.1"
                     /translation="MNDDALLAALGERISALAPALIDIRRDLHAHPELSREETRTTQS
                     VADHLAAAGVRVRLLPGTGLLADVGPEDAVHRIALRADMDALPVRERTGLDFASTTPG
                     VCHACGHDVHVTALIGA"
     gene            410..1357
                     /locus_tag="PL014_12590"
     CDS             410..1357
                     /locus_tag="PL014_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT57973.1"
                     /translation="MATSDVDVIRTTGLQKAFGRTQALDGLDLTVAHGEVHGFLGPNG
                     SGKSTAIRVLLGLLRGDGGTASVLGRDPWRDATELHRSIAYVPGDVVLWPTLSGGEVI
                     DLLGRLRGGIDDDRRRRLVARFELDETKKGRAYSKGNRQKVALVAALASDAPLLILDE
                     PTSGLDPLMEEVFQAELAELRAQGRTVLLSSHILSEVERACDRVSIVRSGRIVDSGSL
                     ADLRHLTRTRVHAVLRDAVAPADVAGWAGIHDIDLVSSTASGGSGGGDAGSELSCSVD
                     SAHLGDLVEHLGRRGVLALTCQPPTLEELFLARYREPSP"
     gene            1354..>2822
                     /locus_tag="PL014_12595"
     CDS             1354..>2822
                     /locus_tag="PL014_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide antibiotic transporter"
                     /protein_id="HOT57974.1"
                     /translation="MTTRLAGAPELFRLAWRRDRILVPASVLGLVALSVGSAQATLAL
                     YPDDEAAATGLAGVLTNPSVIAMYGPLASQTADALAVFKTVMMGAFLTAVLGFVVVRR
                     HTRTEEDEGRHELVGSGVVGRWSALAASVALACLSVIAASALSAVGLAALGMDGAGSA
                     AFAVAWLSAGLATVGVTAVAVQLASTSRGAAGLGFGFLAAAYALRAVADSSDAGTLLH
                     ALGWLSPLGWAGRVEAYGANRQWVLLLGLGALVIGVAVGLAVLDRRDLGAGLIPTRSG
                     PSRAGALLASPLGLVTRLARGTIIGWSIGITLGAVAIGSLLGSIADLADDEVIQEFLH
                     QLGGSVGTIEDIFVATEVRFVAVAVAAAGVALLLRLVGAERGGLGEVVLTTPVSRTRW
                     YAAHVVVSLVLVTVLMALFGVVLGLVGSAVSPLGPSVGAVVAASLATAPAVWVVVGVT
                     AFLCGTSPRFAPFAWGVLLVAFVVGELGTTMRLPSWVIDA"
BASE COUNT          312 a          973 c         1085 g          452 t
ORIGIN      
        1 tcgccccgat gagcgccgtg acgtgcacgt cgtgcccgca ggcgtggcac accccaggag
       61 tggtggaggc gaagtcgagg ccggtgcgct cgcgaaccgg caaggcgtcc atgtccgcgc
      121 gcagcgcgat ccggtgcacg gcatcctcgg gcccgacgtc ggcgagcagg ccggttccgg
      181 gcagcaggcg aacgcgcacc ccggcggcgg cgaggtggtc ggccacggac tgcgtcgtgc
      241 gcgtctcctc gcgcgacaac tcggggtggg cgtgcaggtc acgccggatg tcgatgagtg
      301 cgggtgcgag tgcggagatg cgctcgccga gcgcggcgag gagagcatcg tcgttcatag
      361 gaagccgagt gtatccacgt ctgacgcggg cgggcgtagc gtaggggcca tggcgacgtc
      421 agacgtcgat gtcatccgca cgaccggcct gcaaaaggcg ttcggccgca cgcaggcctt
      481 ggacgggctc gacctcaccg tcgcccacgg cgaggtgcac ggcttcctcg gaccgaacgg
      541 gtcgggcaag tcaacggcga tccgggtcct gctcgggctg ctgcgcggcg acggcggcac
      601 ggcctccgtc ctcggtcgcg atccctggcg cgacgcgacc gagttgcacc gctccatcgc
      661 ctacgtgccg ggcgacgtcg tcctgtggcc gaccctcagt ggcggcgagg tcatcgacct
      721 gctcgggagg ctgcgcggcg gcatcgacga cgaccgtcgc cgccgcctcg tggcacgctt
      781 cgagctcgac gagacgaaga aggggcgcgc ctactccaag ggcaaccggc agaaggtggc
      841 gctcgtcgcg gcgctggcca gcgatgcacc gctgctcatc ctcgacgagc cgacgtcggg
      901 gctggacccg ctcatggagg aggtcttcca ggccgagctc gccgagctcc gggcgcaggg
      961 ccgcaccgtg ctgttgtcga gccacatcct cagcgaggtc gaacgggcct gcgaccgggt
     1021 cagcatcgtg cgctcgggcc ggatcgtcga cagcggcagc ctcgccgacc tgcgtcacct
     1081 gacccgcacc cgggtgcacg ccgtgctgcg cgacgcggtg gcgccggcag acgtcgcggg
     1141 gtgggccggc atccacgaca tcgacctcgt gagcagcacc gccagcggcg gttccggcgg
     1201 cggcgatgcc ggtagcgagc tgagctgctc ggtggactcc gcccacctcg gtgacctcgt
     1261 ggagcacctc ggccgccgcg gggtgctggc cctgacctgc cagccgccga cgctcgagga
     1321 gctcttcctc gcgcgctatc gagaaccgtc accatgacca cccggctggc tggcgcgccc
     1381 gagctgttcc ggctcgcgtg gcggcgcgac cgcatcctcg tgcctgccag cgtgctcggg
     1441 ctggtcgccc tctcggtcgg ctcggcgcag gcgacgctcg cgctctaccc cgacgacgag
     1501 gcagcggcga cgggtcttgc cggcgtcctc acgaatcctt cggtgatcgc gatgtacgga
     1561 ccgctggcgt cgcagaccgc ggacgcgctg gccgtgttca agacggtgat gatgggcgcc
     1621 ttcctcacgg ccgtgctcgg cttcgtcgtc gtgcggcggc acacccgcac ggaggaggac
     1681 gagggtcggc acgagctcgt gggctccgga gtcgtcggcc gctggtccgc cctcgcggcg
     1741 tcggtggcgc tggcctgcct gtcggtcatc gcggcgagcg ccctgtcggc ggttggtctg
     1801 gccgcgctcg gcatggacgg cgccgggagc gcggccttcg ccgtcgcgtg gctctcggcc
     1861 gggctggcga ccgtgggggt gacagccgtg gcggtgcagc tggcgtcgac ctcgcgcggc
     1921 gcggccggcc tgggcttcgg tttcctcgct gcggcgtacg cgctgcgcgc cgtcgctgat
     1981 tcgtcggatg ccgggactct ccttcacgct ctcggctggc tgtcgccgtt gggctgggcg
     2041 gggcgcgtgg aggcctacgg ggcgaaccgg cagtgggtgc tgctgctcgg cctcggcgcc
     2101 ctggtgatcg gtgtcgcggt gggcctggcg gtgctggacc ggcgcgacct cggcgccggg
     2161 ctgatcccga cgcgcagcgg cccgtcgagg gctggcgcgc tgctggcctc tcccctgggg
     2221 ctcgtcaccc gcctcgcccg ggggaccatc atcggctgga gcatcggcat caccctgggg
     2281 gccgtggcca tcgggtcgct gctcggctcg atcgcggacc tggccgatga cgaggtcatc
     2341 caggagttcc tgcaccagct cggcggctcg gtgggcacga tcgaggacat cttcgtcgcg
     2401 acggaggtgc ggttcgtcgc cgtggcggtc gcggcagccg gcgtggccct gctgctgcgc
     2461 ctcgtggggg ccgagcgcgg cgggctcggt gaggtcgttc tcaccacccc cgtcagccgc
     2521 acgcgctggt atgccgcaca cgtcgttgtc tcgctcgtgc tggtcaccgt gttgatggcg
     2581 ctgttcgggg tcgtcctcgg gctggtcggg tcggccgtga gccctctggg gccgtcagtt
     2641 ggtgcagtgg tcgcagcgtc gttggcgacg gcgcccgccg tgtgggtggt cgtcggggtg
     2701 acggcgttcc tgtgtggcac atcaccgcgg ttcgccccgt tcgcgtgggg cgtgctgctc
     2761 gtggcgttcg tcgtcggcga gctggggacg acgatgcgcc tgccgtcatg ggtgatcgac
     2821 gc
//
LOCUS       DAOSOC010000250         2719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_37385_length_2719_cov_9.779655, whole genome shotgun sequence.
ACCESSION   DAOSOC010000250 DAOSOC010000000
VERSION     DAOSOC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37385_length_2719_cov_9.779655"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..659
                     /locus_tag="PL014_12600"
     CDS             75..659
                     /locus_tag="PL014_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT57975.1"
                     /translation="MTTLRARFAAFVLRVTGWTAVGEVPRTGILVGAPHTSNWDFVMM
                     LLIMWRGGVSPRVLIKQEIFRGPLGWLLPRLGGIPLDRSNPGAVIRHLVAQARTGEPF
                     LLILAAEGTRSKGEYWKSGFWRIARSTGLPISLAFVDGPTKTSGFGPTFKPTKDVVAD
                     MDLVREFYADKRGIHPERRTEPRLREELPSGEAS"
     gene            complement(656..>2719)
                     /locus_tag="PL014_12605"
     CDS             complement(656..>2719)
                     /locus_tag="PL014_12605"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOT57976.1"
                     /translation="YAQGFARVASCTVPVALADAATNAERIIQQVKACHDDGVAVALF
                     PELSLSGYAIDDLLLQDVLLDAVDEAVVTIAEATAKLLPLVVVGAPVRHGNRLYNCGI
                     VIHRGEILGVSPKSFLPNYREFYEKRHFASGAGHQGEFLVRPHWPGADADGEIPFGPD
                     LLFTADDHPDLAVHVEICEDMWVPVPPSHEAALAGATVLLNLSASPITVGRAEDRHLL
                     ARSASARCLAAYLYAAASDGESTTDLSWDGMTMVYEMGDLLGESDRFPDGPRRTVVDV
                     DLDRLRQERIRQGSFHDNAEAYAADQGGFRVVPFTLRPPSGDLGLRRAVDRFPFVPDD
                     EARLAQDCYEAYNIQVSGLEQRMRAIGGGDPARMPTIVIGVSGGLDSTHALIVAAKAC
                     DRLDLPRTHIIGFTMPGFATSAGTKSNAIHLMESLGITWEELDIRPAATQMLKDLGHP
                     FGRGEEVYDVTFENVQAGLRTDYLFRAANQRGGIVLGTGDLSELALGWCTYGVGDQMS
                     HYGVNTGVPKTLMQHLIRWVVSSQQFEGPVNGTLLEILDQEISPELVPTKEGERIQST
                     EDSVGPYALQDFTLFHVLRRGYRPSKIAFLAEHAWSDAGSGLWPAGFPEGSRPEYDLA
                     TIRRWMRTFHWRFFANQFKRSAIPNGPKVVSGGSLSPRGDWRMPSDAGSGPWLAELEA
                     NVPEA"
BASE COUNT          391 a          929 c          995 g          404 t
ORIGIN      
        1 gtcgccttat gtccggcaga ctggtgcccc aaaccgccaa cctgccggac gttgtgctgg
       61 caccaggagc tgagatgacc acgctgcgcg ctcgattcgc ggccttcgtg ctgcgcgtca
      121 ccggctggac ggccgtcggg gaggtgccgc gaaccggcat cctcgtcggt gccccgcaca
      181 cctccaactg ggacttcgtc atgatgctgc tcatcatgtg gcgcggcggc gtctcccccc
      241 gggtgctcat caagcaggag atcttccgcg gacccctcgg gtggctgctg ccccggctcg
      301 gcggcatccc gctcgaccgc tccaacccgg gcgctgtcat ccgacacctg gtcgcgcagg
      361 cccgcaccgg cgaaccgttc ctgctgatcc tcgccgccga gggcacccgc agcaagggtg
      421 agtactggaa gtcggggttc tggcggatcg cccgctcgac cgggctgccg atctcgctcg
      481 cgttcgtcga cgggccgacc aagacctcag gtttcggccc gaccttcaag cccacgaagg
      541 acgtcgtcgc cgacatggac ctcgtgcgcg agttctacgc cgacaagcgc ggcatccacc
      601 ccgagcggcg caccgagccc cgcctgcgcg aagagctgcc ctccggcgag gcgagctagg
      661 cctcggggac gttggcctcg agctcggcca gccacgggcc ggacccggcg tccgacggca
      721 tccgccagtc tccgcgcggt gacagcgagc cacccgagac gaccttgggc ccgttcggga
      781 tcgccgagcg cttgaactgg ttggcgaaga agcgccagtg gaaggtccgc atccaccgcc
      841 ggatcgtcgc gaggtcgtac tccgggcgcg aaccctcggg gaagccggcc ggccacagcc
      901 cgctcccggc atccgaccac gcgtgctcgg cgaggaaggc gatcttgctc gggcggtagc
      961 cgcgccgcag gacgtggaag agcgtgaagt cctgaagggc gtaggggccg acggagtcct
     1021 cggtcgactg gatgcgctcg ccctccttgg tggggacgag ctcggggctg atctcctggt
     1081 cgaggatctc caggagcgtg ccgttgaccg gcccctcgaa ctgctgcgac gacaccaccc
     1141 agcggatgag gtgctgcatc agcgtcttcg gcaccccggt gttgaccccg tagtgcgaca
     1201 tctggtcgcc gacgccgtac gtgcaccagc cgagtgccag ctcggacagg tcgccggtgc
     1261 cgagcacgat gccgccgcgc tggttggccg cccggaagag gtagtcggtg cgcagcccgg
     1321 cctgcacgtt ctcgaaggtg acgtcgtaga cctcctcgcc gcggccgaac gggtggccga
     1381 ggtccttgag catctgggtc gccgcggggc ggatgtcgag ctcctcccac gtgatgccga
     1441 gcgactccat gaggtggatg gcgttgctct tggtgccggc gctcgtcgcg aagcccggca
     1501 tcgtgaagcc gatgatgtgg gtgcgcggca ggtcgagccg gtcgcacgcc ttggccgcga
     1561 cgatcagcgc gtgggtggag tcgagcccgc ccgagacgcc gatgacgatc gtcggcatcc
     1621 gggccgggtc gccgccgccg atggcgcgca tccgctgctc gagcccggag acctggatgt
     1681 tgtaggcctc gtagcagtcc tgggccaggc gggcttcgtc gtcgggcacg aaggggaagc
     1741 ggtcgacggc gcggcgcagc ccgaggtcgc cgctcggtgg acgcagcgtg aacggcacga
     1801 cgcggaaccc gccctggtcg gccgcgtagg cctcggcgtt gtcgtggaag ctgccctggc
     1861 ggatgcgctc ctggcgcagc cggtcgaggt cgacgtcgac gaccgtgcga cgggggccgt
     1921 cggggaaccg gtcggactcc ccgagcaggt cgcccatctc gtagaccatc gtcatgccgt
     1981 cccacgacag gtcggtcgtc gactccccgt cgctcgcggc ggcgtagagg taggcggcga
     2041 ggcagcgggc gctcgccgaa cgcgccagca ggtggcggtc ctcggcgcgt cccaccgtga
     2101 tgggggaggc cgagaggttg agcagcacgg tcgcgccggc cagggcggct tcgtggctcg
     2161 gtggcaccgg cacccacatg tcctcgcaga tctcgacgtg cacggcgagg tcggggtggt
     2221 cgtcggcagt gaacagcagg tcggggccga aggggatctc gccatcggcg tctgcgccgg
     2281 gccagtgcgg ccgcacgagg aactcgccct ggtgaccggc gcccgaggcg aagtgccgct
     2341 tctcgtagaa ctcgcggtag ttcggcagga acgacttggg ggagaccccg aggatctcgc
     2401 cgcggtggat gacgattccg cagttgtaga gccggttgcc gtggcgcacc ggggccccga
     2461 cgacgaccag cggcagcaac ttggcggtcg cctcggcgat ggtcacgacg gcctcgtcca
     2521 cggcatccag caggacgtcc tgcaggagga ggtcgtcgat ggcgtacccc gacagactga
     2581 gctcggggaa cagggccacc gcgacgccgt cgtcgtggca ggccttgacc tgttggatga
     2641 tccgctcggc gttggtggcc gcgtcggcca gcgccacggg cacggtgcac gaggcgacgc
     2701 gggcgaaccc ctgggcgta
//
LOCUS       DAOSOC010000251         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_37870_length_2693_cov_6.268006, whole genome shotgun sequence.
ACCESSION   DAOSOC010000251 DAOSOC010000000
VERSION     DAOSOC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37870_length_2693_cov_6.268006"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1116
                     /locus_tag="PL014_12610"
     CDS             163..1116
                     /locus_tag="PL014_12610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT57977.1"
                     /translation="MRSFTTDEDPMTRSTLPFLAAASALVLALSACSADAEVAAPSTP
                     AAPSASSSPSPTPSDAAPERLYVALGDSLAAGYQPGGTELRDTAYPALTANRLEAAGA
                     SLEVENLGCSGETTTSLIEGGKCTFDEGSQLEQAEAVLAERKGEVALVTIDIGGNDLL
                     ACVRGGAQIDAACVTKGVATVEKNLPTILERLRTAAGPEVPVLVLGYYNPWVAAQTLD
                     QPVEGVEEAAQGYADLSDAIEAAADGAGATFVSLDEAFATNDTTPTEIGGRTIPENAA
                     RVCTLTNLCTARDIHFSDEGAATVARVLADAATTAGVGAAS"
     gene            1170..1940
                     /locus_tag="PL014_12615"
     CDS             1170..1940
                     /locus_tag="PL014_12615"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT57978.1"
                     /translation="MRIARFTTGEDPAYGLVDGAGEKIAEITGDPLYQRIELTGAVHQ
                     VADVRLLAPVIPRSKVIGIGKNYADHALEMGGEAPSTPLMFLVPNTAVVGPGDPVVMP
                     PQTSEVSYEGELAVVIGRLCKDVEASEALSAVFGYTVANDVTARDLQRTDGQWARAKG
                     FDTFCPLGPWIETDLDPAGQRIVTMLDGDIVQDGATSDMVHDVAALIAFASQAFTLLP
                     GDVILTGTPAGVGLVEAGQRVDVDIDGIGVLSNPFVRH"
     gene            2185..>2693
                     /locus_tag="PL014_12620"
     CDS             2185..>2693
                     /locus_tag="PL014_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOT57979.1"
                     /translation="MSLFNLAFVRQHGGRFVLRIEDTDRTRFRADSEQQVFDTLSWLG
                     LTWDEGPDIGGPFEPYRQSERLDTYRPYVERLLESGHAYHCWCTPGRLAQMREAQARD
                     KKPTGYDRMCHGLTEQERAELPGFSETPVVRMFVPDDVPLVFDDLIRGTVSAPRPDDQ
                     VIFKADGFPT"
BASE COUNT          409 a          990 c          918 g          376 t
ORIGIN      
        1 cgagggtcag cacgcggtca gtcaccgcac cacggtaccc acgcaccggc ggtgcggtgc
       61 tcgctgccgg gctcggtggt gtcgaaggac gggaacccga acgggtccgc tgctcgctga
      121 ggggacaatg gaggccaccg actccgagcc cagcgtcatc cgatgcgttc cttcacgacc
      181 gacgaggacc ccatgacccg ctccaccctg ccgttcctcg ctgcggcgag cgcgctcgtg
      241 ctcgcgctct cggcctgcag cgccgacgcc gaggtggctg ccccgagcac cccggcggcg
      301 ccctccgcgt ccagctcgcc gtcacccact ccgtcggatg ccgcgcccga gcgcctctac
      361 gtcgccctgg gcgactcgct cgccgcgggc taccagccgg gtggaaccga gctgcgcgac
      421 acggcctacc cggcgctgac ggcgaaccgg ttggaggctg cgggtgcgtc tctcgaggtc
      481 gagaacctgg gctgcagcgg cgagacgacg acctcactca tcgagggtgg caagtgcacc
      541 ttcgacgagg gcagccagct cgagcaggcc gaggcggtgc tcgccgagcg caagggtgag
      601 gtggcgctgg tcacgatcga catcggtggc aacgatctcc tcgcgtgcgt gcgcggcggt
      661 gcccagatcg acgcggcctg tgtgaccaag ggggtcgcca ccgtcgagaa gaacctgccg
      721 accatcctcg agcgcctgcg caccgcggcg gggccggagg ttcccgtgct cgtgctcggc
      781 tactacaacc cctgggtcgc ggcccagacg ctggaccagc ccgtcgaggg tgttgaggag
      841 gcggcccagg gctatgccga cctcagcgac gcgatcgagg cggcggccga tggcgccggg
      901 gcgaccttcg tcagtctcga cgaggcgttc gccacgaacg acaccacacc gaccgagatc
      961 ggtgggcgca ccattcccga gaacgccgcc cgggtctgca ccctgaccaa cctctgcacc
     1021 gcccgcgaca tccacttctc ggacgagggt gccgccaccg tggcgcgggt gctcgccgac
     1081 gcggcgacca ccgcgggtgt gggtgccgcg agctgacggg tcaggccctg cacatcgtgt
     1141 gaaccggtga cgggcctagg cttgtgccca tgcgcatcgc gaggttcacg acgggtgagg
     1201 acccggccta cgggctggtc gacggggcag gggagaagat cgccgagatc accggcgacc
     1261 cgctctacca gcgcatcgaa ctgaccggtg ccgtccacca ggtggccgac gtgcgactgc
     1321 tcgcccccgt catcccccgc agcaaggtga tcggcatcgg caagaactac gccgaccacg
     1381 ccctcgagat gggcggggag gccccgtcga cccccctgat gttcctcgtg ccgaacacgg
     1441 ccgtcgtcgg gccgggtgac cccgtcgtga tgcctcccca gacgagcgag gtgagctacg
     1501 agggtgagct ggccgtggtc atcgggcggt tgtgcaagga cgtcgaggcg agcgaggccc
     1561 tctctgcggt cttcggctac accgtcgcca acgacgtgac cgcccgtgac ctccagcgca
     1621 ccgacggcca gtgggcgcgg gccaagggct tcgacacctt ctgccccctc gggccgtgga
     1681 tcgagaccga cctcgacccc gccggccagc ggatcgtcac catgctcgac ggtgacatcg
     1741 tgcaggacgg cgcgacgtcg gacatggtgc acgacgtcgc cgccctgatc gcgttcgctt
     1801 cccaggcctt caccctgttg ccgggagacg tcatcctcac cgggacgccc gccggagtcg
     1861 gcctcgtgga ggcggggcag cgcgtcgacg tcgacatcga cggcatcggg gtgctcagca
     1921 atccgttcgt gcgtcactag gctgtcgccc accatgacag agccgaccta tgaaggccgc
     1981 cacgccggcc cccccgccga tgagacctcg gatgccgccg ggtcaccgag cgacgcacac
     2041 cccgacgggc gccaccgacc cgcagagagc gagcagtccg tgtccgagac ctccgcagca
     2101 gcacccgtcg gcgccctgac cggggccgtg cgccttcggg tcgcaccctc gcccacgggt
     2161 gacccccacg tcggtacggc ctacatgtcg ctgttcaacc tggcgttcgt gcgccagcac
     2221 ggggggcgct tcgtcctgcg catcgaggac accgaccgca cgcggtttcg cgccgactcc
     2281 gagcagcagg tgttcgacac gttgtcgtgg ctcgggctga cctgggacga gggccccgac
     2341 atcggtggcc ccttcgagcc ctatcgccag agcgagcgcc tcgacaccta ccggccctac
     2401 gtcgagcggc tgctcgagtc cggccacgcc taccactgct ggtgcacacc ggggcggttg
     2461 gcgcagatgc gcgaggcgca ggccagggac aagaagccga ccggctatga ccgcatgtgc
     2521 cacgggctca cggagcagga gcgggcagag cttcccggct tctccgagac gcccgtcgtg
     2581 cgcatgttcg tgcccgacga cgtcccgctg gtcttcgacg acctcatccg cggcacggtg
     2641 tcggcaccgc gcccggacga ccaggtcatc ttcaaggccg acggcttccc cac
//
LOCUS       DAOSOC010000252         2643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_38768_length_2643_cov_6.179675, whole genome shotgun sequence.
ACCESSION   DAOSOC010000252 DAOSOC010000000
VERSION     DAOSOC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38768_length_2643_cov_6.179675"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PL014_12625"
     CDS             <1..1052
                     /locus_tag="PL014_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT57980.1"
                     /translation="DSPLYGMFAIRSAVAAIGPHDVTLTTGDTLSADLVVAAIGVRPE
                     ITLAEQAGLAIGERGGILVDDELRTSDPAVLAVGDAVEKTDAVDGTAALIPLANTANL
                     QGRRAADVIAGQAARNRPVLGTAIVGVLGLQIASTGWSEKRLRAAGRDVRAIHTHPAS
                     HATYYPGADTIALKLLVDPHTDEILGAQGVGESGVDKRIDVIATAMAGRLTASALADL
                     ELAYAPAFSSAKDPVNMLGHIAQNLRTGTSRSIQWHEVADAVAAGAVIVDVREASERS
                     GGRLIEGSVHIPVNELRDRLGEIPDGPVIVHCAVGVRGHTAARILTQHGRDVRNLDGG
                     YRTWNAGIRAHVTPA"
     gene            complement(1125..1385)
                     /locus_tag="PL014_12630"
     CDS             complement(1125..1385)
                     /locus_tag="PL014_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOT57981.1"
                     /translation="MELNPDEMQAIVKRLKRAQGQIGGILKMIDDGRECQDIVTQLAA
                     VSKAVDRAGFAVIALGLRQCIVDPDSHEMDVDSMEKLFLSLA"
     gene            complement(1430..1819)
                     /gene="trxA"
                     /locus_tag="PL014_12635"
     CDS             complement(1430..1819)
                     /gene="trxA"
                     /locus_tag="PL014_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT57982.1"
                     /translation="MATVELTADTFEKTITTSDIVLVDWWAAWCGPCRQFAPTYEKSS
                     EKHADVVFGKVDTEAEQALAGAAQITSIPTIMAFREGVLVYRQAGALPPAGLEQVIDG
                     IKALDMDDVRAQMQAQAQQAAAQQSQA"
     gene            1960..>2643
                     /locus_tag="PL014_12640"
     CDS             1960..>2643
                     /locus_tag="PL014_12640"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HOT57983.1"
                     /translation="MSSPLTPSLASALEAVLDGLPADALRRATASLIEAYRSGTPPTA
                     QVLHDTTTAAAYAAYRMPATHAAVTRAVRHTTDLVPGLRVGSVVDIGGGTGAATWAVA
                     QAFPDLTTATVADGSTDALATGRRIARHGPPPVAAATWQRTHLRADTVLPQADLAVVS
                     YLLGELAGPLQEAVVDAAATASPTLLIIEPGTPRGYAAVLAARTRLTTTGWHVLAPCP
                     QDGPCPIADR"
BASE COUNT          388 a          983 c          902 g          370 t
ORIGIN      
        1 tcgacagccc gctctacggg atgttcgcga tccgttcggc ggtcgcggcg atcgggccgc
       61 acgacgtcac cctcaccacc ggcgacaccc tctctgccga cctcgtcgtc gccgccatcg
      121 gtgtgcgtcc ggagatcacc ctcgccgagc aggcggggct cgcgatcggc gagcgcggcg
      181 gcatcctcgt cgatgacgag ctgcgcacga gcgaccccgc cgtcctcgcc gtcggtgacg
      241 cggtcgagaa gacggatgcc gtcgacggca ccgcagcgct catccccctc gccaacaccg
      301 cgaacctcca gggccgtcga gccgctgacg tcatcgcagg tcaggcagcg cgcaaccgac
      361 ccgtgctcgg gaccgccatc gtcggcgtcc tcgggctcca gatcgcgtcg accgggtggt
      421 cggagaagcg gctgcgagcc gcgggacggg acgtgcgcgc catccacacg cacccggcat
      481 cccacgcgac gtactacccg ggcgccgaca ccattgccct gaagttgctc gtggacccgc
      541 acaccgacga gatcctgggt gctcagggcg tcggcgagtc gggagtcgac aagcgcatcg
      601 acgtcatcgc gaccgccatg gccgggcggc tgacggcgag cgccctggcc gatctcgaac
      661 tcgcctacgc gccggcgttc tcgtcggcga aggacccggt caacatgctc gggcacatcg
      721 cgcagaacct gcgcaccggc acgagccggt cgatccagtg gcacgaggtg gcggatgccg
      781 tggccgcggg ggccgtgatc gtggacgtgc gcgaggcctc ggagcgctcg ggtgggcgct
      841 tgatcgaggg gtcggtgcac atcccggtga acgagcttcg ggatcgcctc ggcgagatcc
      901 ccgacggccc ggtcatcgtg cactgcgcgg tcggggtgcg cgggcacacg gcagcgcgga
      961 tcctgaccca gcacggaaga gacgtgcgca acctcgacgg cggctaccgc acctggaacg
     1021 ccggcatccg cgcacacgtg acgcccgcct gacccggcgt accgagatcg accaataggt
     1081 cactcggggt acgggcggtc agcgccccca ccgatccccg aggctcacgc cagcgagagg
     1141 aagagcttct ccatggagtc gacgtccatc tcgtgggagt cggggtcgac gatgcactgg
     1201 cgcaggccga gcgcgatgac ggcgaacccg gcccggtcca cggccttgga gaccgcagcc
     1261 agctgggtga cgatgtcctg gcactcgcgg ccgtcgtcga tcatcttgag gatgccgccg
     1321 atctggccct gggcgcgctt gaggcgcttg acgatcgcct gcatctcgtc ggggttgagc
     1381 tccatgccgg ggccctttct ctcggatcga acggggtcgg gctgctgggt caggcctggg
     1441 actgctgggc cgcggcctgc tgggcctggg cctgcatctg ggcacggacg tcgtccatgt
     1501 ccagggcctt gatgccgtcg atgacctgct cgaggccggc cggcggcagc gccccggcct
     1561 gacggtagac gaggacaccc tcgcggaagg ccatgatcgt ggggatcgag gtgatctggg
     1621 cggcaccggc gagagcctgc tcggcctcgg tgtcgacctt gccgaagacg acgtcggcgt
     1681 gcttctcgct cgacttctcg taggtcggcg cgaactggcg gcacgggccg caccacgccg
     1741 cccaccagtc gacgagcacg atgtcgctcg tcgtgatggt cttctcgaag gtgtcggcgg
     1801 tgagctcgac ggtggccatg gtgaacactc ctgaagtgat ggtcggctgg ggtccagcga
     1861 cgtcaacagc atacccctgt gggtatattc ccgacaccgc agactcagcc agaacgccgc
     1921 aactcttcgc gatcatcccg ctggcggcac aatgtgcgcg tgtcgagccc cctcaccccc
     1981 agcctcgcct cggccctcga ggccgtgctc gacgggcttc cggccgacgc cctgcggcgc
     2041 gccacggcat ccctcatcga ggcctaccgc agcggtaccc cgccgacggc ccaggtcctg
     2101 cacgacacga ccacggccgc cgcctacgcg gcgtaccgga tgccggccac gcacgccgcg
     2161 gtgacgcgcg ccgtgcgcca caccaccgac ctggtgcccg gcctgcgcgt cggctctgtc
     2221 gtcgacatcg gcgggggcac cggggcggcg acctgggccg tggcgcaggc gttcccggac
     2281 ctcaccaccg caacagtcgc cgacgggtca acagatgccc tggcaacggg tcggcggatc
     2341 gcccgccacg gcccgccacc cgtggccgca gccacctggc aacgcaccca cctgcgcgcc
     2401 gacaccgttc tgccacaggc ggatctggcc gtggtcagct acctgctcgg ggaactggcc
     2461 ggccccctcc aggaggcggt cgtcgatgcc gccgcgacgg catccccgac gctcctcatc
     2521 atcgaacccg gcacaccgcg cgggtacgcc gccgtgctcg cagcccgcac ccgcctcacg
     2581 accaccgggt ggcacgtgct cgccccctgc ccgcaggacg ggccctgccc gatcgccgac
     2641 cgc
//
LOCUS       DAOSOC010000253         2624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_39112_length_2624_cov_6.471390, whole genome shotgun sequence.
ACCESSION   DAOSOC010000253 DAOSOC010000000
VERSION     DAOSOC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39112_length_2624_cov_6.471390"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PL014_12645"
     CDS             complement(<1..790)
                     /locus_tag="PL014_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT57984.1"
                     /translation="MIDAAGLRVMKAIADEGSFTAAATSLGYTQPAISQMVRRLEQRT
                     GTVLVERVGRNVRLTEAGQVLARHATPVLAAIDAAEEEVAAIAGLRSGRVRLMAFPSA
                     SATLVPRALSLVKKRFPDISITLTEAEPPESLAALKAGECDLAVAFAYEGTDIGRGEE
                     DLDQFVITNLLDDEVRLAVPEGHSVSAQENVDLSVLADEPWIAGCPRCRGHLLQLTHD
                     AGFRPDVAYETEDYVAVMGLVAEGLGVALIPDLILRTVHHPDVVA"
     gene            969..1880
                     /locus_tag="PL014_12650"
     CDS             969..1880
                     /locus_tag="PL014_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HOT57985.1"
                     /translation="MPDAVAPLTSALSAAVPLTEASLAPLAAMLFDHPGTLVLTGAGM
                     STDSGIPDYRGPDGQRRVTPMQHGEFVGSSAARQRYWARSFIGWQRFSHAEPNDCHHA
                     VAALQARGVLGPVITQNVDGLHQAAGSRDVTELHGTLAEVLCLTCGTRTDRDLLQARM
                     AEQNPGFEALASGEASDGSRVSSQIRPDGDIVLDDALVTAFHLPHCTTCGADTLKPDV
                     VFFGGSVPRERVDACYALTDEAPALLVLGSSLAVMSGLRFVRHAAKRGIPVMAITRGP
                     TRGDDLMTVRVDAPLRPSLRALVDHQA"
     gene            2028..2453
                     /locus_tag="PL014_12655"
     CDS             2028..2453
                     /locus_tag="PL014_12655"
                     /inference="COORDINATES: protein motif:HMM:NF025444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HOT57986.1"
                     /translation="MGATGSGPFENDDALDFLGDLEDLPRDQRPAVVLAALETVLRSS
                     GYIEAPEMCEAVAGAAAVGASVNPAAAQGEPYLPQWLIDEPLPTGDQELVEKSRQVLR
                     RAVRSHANEWWELWEEAGLAGDVTASCRRALAWLGDRDD"
BASE COUNT          384 a          915 c          942 g          383 t
ORIGIN      
        1 gggcgacgac gtcggggtgg tgcaccgtgc gcaggatgag gtcggggatg agcgccaccc
       61 cgagaccctc ggcgaccagg cccatgacgg ccacgtagtc ctcggtctcg taggcgacgt
      121 caggccggaa cccggcgtcg tgcgtcagct ggagcaggtg tccgcgacag cgcgggcagc
      181 cggcgatcca cggttcgtcg gcgagcaccg agaggtcgac gttctcctga gccgacacac
      241 tgtggccctc gggcacggcg aggcgcacct cgtcgtcgag caggttcgtg atgacgaact
      301 ggtcgaggtc ctcctcgccc cggccgatgt cggtgccctc gtaggcaaat gccacggcga
      361 ggtcgcactc gccagccttc agtgcggcca ggctctcggg gggctcggcc tcggtgaggg
      421 tgatgctgat gtcggggaag cgcttcttga ccagcgacag cgcgcgcggc accagggtcg
      481 ccgacgccga ggggaaggcc atgagccgca cccgcccgct gcgcagtccg gcgattgccg
      541 cgacctcttc ctcagccgcg tcgatggccg cgagcaccgg ggtggcgtgg cgggcgagca
      601 cctggccggc ctcggtgagg cggacgttgc ggccgacccg ctcgacgagc acggtgccgg
      661 tgcgctgctc gaggcgccgc accatctgcg agatcgccgg ctgcgtgtag cccagcgagg
      721 tggcggccgc ggtgaagctg ccctcgtcgg cgatggcctt catgacccgg agtcctgcgg
      781 cgtcgatcat gcccgcatcc tataacgccg agttatctcc ggctatgagt tctgcacccc
      841 gcgtgatccg acttcctgcg ctgcagccac ctgactgggt cgtcccgagg tggttggcgt
      901 gcaagaagtc aggggtgcga ggtggctgga gtgtaatcag tcgcgggtgg ggtgggagga
      961 tccacgccat gccggatgcc gtcgcgccac tcacctccgc gctctccgct gcggtaccac
     1021 tcaccgaggc ttcgctggcc ccgttggccg cgatgctgtt cgaccacccg ggcacgctgg
     1081 tgctcaccgg ggccgggatg tcgaccgaca gcggcatccc cgactaccgc ggcccggacg
     1141 gccaacgcag ggtcaccccc atgcagcacg gcgagttcgt cggcagcagc gcggctcggc
     1201 agcgctactg ggcgcgctcg ttcatcggct ggcagcggtt cagccacgcc gagcccaacg
     1261 actgccacca cgcggtggcc gccctccaag cgcgtggtgt gctcgggccg gtcatcaccc
     1321 agaacgtcga cgggctgcac caggccgcgg gcagtcgtga cgtcaccgaa ctgcacggca
     1381 cgctggccga ggtgctgtgc ctgacctgcg gcacccgcac tgaccgcgac ctgctccagg
     1441 cccgcatggc cgagcagaat ccggggttcg aggcgctcgc gtcgggcgag gcgtcggacg
     1501 ggtcgcgggt gagcagccag atccgcccgg acggcgacat cgtgctcgac gacgcgctcg
     1561 tcaccgcgtt ccacctgccg cactgcacca cctgcggcgc cgacaccctc aaacccgatg
     1621 tcgtcttctt cggaggctcg gtcccacgag agcgggtcga cgcctgctac gccctgaccg
     1681 acgaggcgcc ggccctgctc gtgctgggtt cgtcgctggc cgtgatgtcg ggactgcggt
     1741 tcgtccggca cgccgcgaag cgcggcatcc ccgtcatggc catcacccgc ggacccacac
     1801 ggggcgacga cctcatgacg gtgcgcgtgg atgccccact tcggccgagc ctgcgcgccc
     1861 tggtcgatca ccaggcctga cccgtggagc gctccagcgc cggccgggca acggtgcgcc
     1921 gccgtgacga tccgcgccat gacgatccgc cacatgacga tgcgtgagtg acgcgggcaa
     1981 cgccccgccg ccgacccgtc gtgctctggc aggcgtacgc tgaaaccatg ggagccaccg
     2041 gatcaggacc cttcgagaac gacgacgcac tcgacttcct cggtgatctg gaggacctgc
     2101 cgcgcgacca gcgaccggcc gtcgtcctgg ctgcgctcga gaccgtgctc cggtcgtcgg
     2161 ggtacatcga ggcgccggag atgtgtgagg ccgtggcggg tgccgcagca gtgggtgcca
     2221 gcgtgaatcc cgctgcggca cagggggagc cataccttcc gcagtggctc atcgacgagc
     2281 ccctgccgac gggcgatcag gagctcgtgg agaagtcgcg gcaggtgctg cgccgcgcgg
     2341 tgcgctcgca cgccaacgag tggtgggagc tgtgggagga ggccgggctc gccggtgacg
     2401 tcacggcatc ctgccgtcgg gccctggcgt ggctcggcga ccgcgacgac tgatgcacct
     2461 gtcagagcgc gccctgacgg cgcgggccga ggttcgcacc acaaggttca ggtgagcacc
     2521 acaaaggaag tggtgctcac ctgaaggttc cggcatcggg cgagaaagtt cttcccggct
     2581 gcagccgcag gcgcggcgca ggaccgtcag acgcggcgca ggac
//
LOCUS       DAOSOC010000254         2622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_39149_length_2622_cov_15.437865, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000254 DAOSOC010000000
VERSION     DAOSOC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39149_length_2622_cov_15.437865"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PL014_12660"
     CDS             <1..558
                     /locus_tag="PL014_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57987.1"
                     /translation="NVCRSPYLERRLRHELAATGIAVESAGTKALAGLEMDDGTRERL
                     DAAGNDASGFRARQVTPEMVAGADLVLTAAREHRTAVAQLHPAALRRVFTVSDFADLV
                     DGLTVVDPDADPLADLDASWVRQVAAAAARRRGSIVARQSGVDITDPIGGPPSAFGQM
                     ANEVDAALVPIVRALRDLPGSPRST"
     gene            complement(565..1953)
                     /locus_tag="PL014_12665"
     CDS             complement(565..1953)
                     /locus_tag="PL014_12665"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOT57988.1"
                     /translation="MELQDYLKVVRKRWRVIVAVVLVFVGLAAAGSLASEKVYESRTQ
                     FFVSTTGAGDNGALLQGSTFTQQRVKSYAQLLTTPRVLGPVAASVGVTGDVAGQVRAT
                     TPPDTVLIDVAVRDTDPARAQAMGEAIATEFPAAVTELESPAGAEASPVKVTVVRPPT
                     TPDSPVSPRPLRNILLGGVLGLLLGLAAALVRETLDKTVKTQDDVKAVTDTPILGSIA
                     YDPDAAKRPLIVEVDPRSPRAEAFRVLRTNLQFVDAAAHPRTIVVTSSLAGEGKSTMA
                     ANLALTMAQAGSRVCLIEADLRRRSKVLEYMGLEGGVGLTDALIGRTDVIDVIQPYGG
                     TNLWVLGAGPIPPNPSELLGSTAMRSLLGHLSSRFDYVVIDAPPALPVTDAVVISKLV
                     DGAIVVAGSGIVERDQLALTLESLESVNGHVLGIVLNRVPVKHSQQYGGYAYVMPEED
                     STRRERKRVEPA"
     gene            complement(1996..>2622)
                     /locus_tag="PL014_12670"
     CDS             complement(1996..>2622)
                     /locus_tag="PL014_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT57989.1"
                     /translation="AALEDLGWGALDGHDGAAGLIPDRSGPSRAGELLASPLGLVTRL
                     ARGTIIGWSIGITLGAVAIGSLLGSVADLADDEVIQEFLRKLGGSAGTIEDIFVATEV
                     RFVAVAVAVAAAGVALLLRLVGAERVGLGEVVLTTAVSRTRWYAAHVALSLVLVTALM
                     ALFGFVLGVVGSAVTPLAPGQHHSPHSGHAKMTGLRPATIWWMGRLAW"
BASE COUNT          408 a          911 c          949 g          354 t
ORIGIN      
        1 aacgtgtgtc gttcgccata cctggagcgc cggctgcgcc acgaactggc cgccacagga
       61 atcgccgtcg agagtgccgg gaccaaggcc ctcgcaggcc tggagatgga tgacggaacc
      121 cgcgaacggc tcgacgccgc cggcaacgat gccagcggat tccgggcccg ccaagtgacc
      181 cctgaaatgg tggccggcgc cgacctcgtg ctgaccgctg cgcgcgagca ccgtacggcc
      241 gtcgcccagt tgcacccggc ggcactgcgc cgggtcttca ccgtgagcga cttcgcggac
      301 ctcgtcgacg ggctcacggt cgtcgacccg gatgcggacc ccctggccga cctcgatgcg
      361 tcctgggtgc gccaggtcgc ggccgccgca gcgcggcgcc ggggctcgat cgtggcgcgc
      421 cagagcgggg tcgacatcac cgaccccatc ggggggccgc ccagcgcctt tgggcagatg
      481 gcgaacgagg tcgacgccgc gctcgtgccg atcgtgcgtg cgctgcgcga cctgcccggc
      541 tcgccccggt cgacctgacc aggttcaggc gggctcgacc cgcttgcgct cgcgccgggt
      601 cgaatcctcc tcgggcatga cgtaggcgta cccgccgtac tgctggctgt gcttgacggg
      661 aacgcggttg agcacgatgc cgagcacgtg cccgttgacc gactccaggc tctcgagggt
      721 gagggccagc tggtcgcgct cgacgatgcc ggagccggcc acgacgatag cgccgtcgac
      781 gagcttggag atgacgacgg catccgtgac tggcagggcc ggcggggcgt cgatgacgac
      841 gtagtcgaag cgcgagctga gatggcccag caggctccgc atcgcggtcg acccgagcag
      901 ctcggacggg ttggggggga tcggcccggc gccgaggacc cagaggttgg tccctccgta
      961 cggctgaatg acgtcgatga cgtcggtgcg gccgatgaga gcgtcggtga ggccgacgcc
     1021 gccctccagg cccatgtact cgagaacctt ggagcgccgc cgcaggtcgg cctcgatgag
     1081 gcacacccga gacccggcct gcgccatggt gagcgccagg ttcgccgcca tcgtgctctt
     1141 gccctcgccg gcgagcgacg aggtcacgac gatggtgcgg gggtgggccg ctgcgtcgac
     1201 gaactgcagg ttcgtgcgca ggacccggaa ggcctcggcg cgcggtgacc gcgggtcgac
     1261 ctcgacgatg agggggcgct tcgcggcatc agggtcgtag gcgatggaac ccagaatcgg
     1321 ggtgtcggtg acggccttga cgtcgtcctg ggtcttgacg gtcttgtcga gcgtctcgcg
     1381 cacgagggcg gcagccagac ccaggagcag gcccagcacc ccgccgagca ggatgttgcg
     1441 gagcggcctg gggctgaccg ggctgtcggg cgtcgtcggc ggccggacga cggtcacctt
     1501 gaccgggctc gcctccgcac ccgccgggct ctcgagctcc gtgaccgcag cggggaactc
     1561 cgtggcgatg gcctcaccca tggcctgggc gcgagccggg tcggtgtcgc gcacggcgac
     1621 gtctatgagc acggtgtcgg gcggggtcgt ggcgcgcacc tggcccgcga cgtccccggt
     1681 cactccgacg gatgccgcca caggcccgag cacgcggggg gtcgtgagca gctgcgcata
     1741 ggacttcacc cgctgctggg tgaacgtgct gccctggagg agcgctccgt tgtcgccggc
     1801 acccgtggtc gacacgaaga actgggtccg cgactcatag accttctcag aggccagcga
     1861 gcccgctgcg gccaggccga cgaagacgag gacgacggcg acgatgacgc gccatcgctt
     1921 ccgaaccacc ttcaggtagt cctgcagctc cacggttctc ctcgggtcga tcgggtacgg
     1981 ccacgacggt tgaggtcacc acgccaggcg ccccatccac caaatggtag ccgggcgaag
     2041 tccggtcatc tttgcgtgac cactgtgcgg tgagtgatgc tgacccggag cgagcggggt
     2101 cacggccgag ccgacgacgc cgaggacgaa cccgaacagg gccatcaacg cggtgaccag
     2161 gacgagcgag agcgccacgt gagcggcata ccagcgcgtg cgacttaccg cggtagtgag
     2221 cacgacctca ccgagcccga cgcgttcggc ccccacgagg cgcagcagca gggccacgcc
     2281 ggctgccgcg accgcgacgg cgacggcgac gaaccgcacc tccgtcgcga cgaagatgtc
     2341 ctcgatcgtg cccgccgaac cgccgagctt gcgcaggaac tcctggatga cctcgtcatc
     2401 ggccagatcc gcgaccgagc cgagcagtga cccgatggcc acggccccca gggtgatgcc
     2461 gatgctccag ccgatgatgg tcccccgggc gaggcgggtg acgagcccca gaggagaggc
     2521 cagcagctcg ccagccctcg acgggccgct gcgatccggg atcagcccgg cagcaccgtc
     2581 gtgtccgtcg agggcacccc accccaggtc ctcgagcgct gc
//
LOCUS       DAOSOC010000255         2605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_39489_length_2605_cov_7.663137, whole genome shotgun sequence.
ACCESSION   DAOSOC010000255 DAOSOC010000000
VERSION     DAOSOC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39489_length_2605_cov_7.663137"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PL014_12675"
     CDS             <1..716
                     /locus_tag="PL014_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HOT57990.1"
                     /translation="AAYYLSKKDRGFDEKIYLLGADHHGYINRLKAIAACAGDDPEHN
                     IEVKIGQLVSIAGERMGKRLGNAIYMDELLEWIGSDAVRYSLARYPADSPLSLDGEEL
                     RKQTNDNPVFYVQYAHARTANVARLAAEDGVHREGGFDAGLLTDPTEAALLAALGDFP
                     RVVAQAAHLLEPHRVARYLEELAGRYHKWYDTCRVRPMSVDEEVTDLHRSRLWLNDAT
                     RQVLANGLDLLGVSAPERM"
     gene            728..2167
                     /gene="lysA"
                     /locus_tag="PL014_12680"
     CDS             728..2167
                     /gene="lysA"
                     /locus_tag="PL014_12680"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT57991.1"
                     /translation="MRIHEAGVLHAEGYGGPPLYLPIPTDVMELLPQLWPGSVSRDDA
                     GRLTVGGVDVVTLAQEHGTAAYVLDEEDFRSRARDFRDSFSAAFEPLAGADVYYAGKA
                     FLCTAVARWVEEEGLSLDVCTGGELAVAQRVKFPGERIGFHGNNKSVAEIRTALTYGV
                     GRIVVDSFQEIERIAAVAAELGVVAPVMVRVTVGVEAHTHEFIATAHEDQKFGFSLGD
                     GAAFEGARRIVAHPEQLRFLGLHSHIGSQIFDTGGFETAAARLIGLHARIAEELGCNA
                     PEIDLGGGYGIAYTSQHTPLPPELLAEQMSHIVRHEFKRIADVDPDGGLPRVSIEPGR
                     AIVGPAAFTLYEVGTVKPVTVADGQVRTYVSVDGGMSDNPRPALYEADFSATLANRSS
                     SAPPVVVRIVGRHCEAGDIVVFDEWLPGDVAPGDLIAVPGTGAYCRALSSQYNHTPRP
                     PVLAVTSERARVIVRRETIDDLLALDVDE"
     gene            2241..>2605
                     /locus_tag="PL014_12685"
     CDS             2241..>2605
                     /locus_tag="PL014_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOT57992.1"
                     /translation="MSVAARPPLRVALLGCGVVGSAVARLLVENSADLASRIGAPLEL
                     VGIAVRRLGRDRSDVPVDPALFTTDAEGLVARADIVIEVVGGIEPTRDLILSAIHHGA
                     SVVSANKALLAEDGPTLYAA"
BASE COUNT          397 a          952 c          858 g          398 t
ORIGIN      
        1 acgccgcgta ctacctgagc aagaaggacc gcggcttcga cgagaagatc tacctgctcg
       61 gcgctgacca ccacggctac atcaaccggc tcaaggccat cgcggcctgc gccggcgatg
      121 accccgagca caacatcgag gtcaagatcg gccagctcgt ctccatcgcg ggggagcgca
      181 tgggcaagcg gctcggcaac gcgatctaca tggacgagct gctcgagtgg atcggttcgg
      241 atgccgtccg gtactcgctg gcgcgctacc cggcagacag cccgctctcc ctcgacggtg
      301 aggagctgcg caagcagacc aacgacaacc cggtgttcta cgtgcagtac gcgcacgccc
      361 gcacggcgaa cgtggcccgg ttggcggccg aggacggcgt gcaccgtgag ggcggcttcg
      421 acgccggcct gctcaccgac ccgaccgagg ccgccctgct ggccgccctc ggcgacttcc
      481 cgcgggtcgt ggcccaggcc gctcacctgc tcgaaccgca ccgggtcgcc cgctacctcg
      541 aggagctcgc cggtcggtac cacaagtggt acgacacctg ccgggtgcgc ccgatgagcg
      601 tcgacgagga ggtcaccgac ctgcaccgca gccggctgtg gctcaacgac gcgacccgcc
      661 aggtgcttgc caacgggctc gacctgctcg gggtcagcgc ccccgagcgc atgtgagggg
      721 ctgaccgatg cgcatccacg aggctggtgt cctgcacgcc gaggggtacg gcggcccacc
      781 gctctacctg cccattccga ccgacgtcat ggagctgctg ccccagctgt ggcccggcag
      841 cgtcagccgt gacgacgccg gccggctcac ggtgggtggc gtcgacgtcg tgaccctggc
      901 gcaggagcac ggcacggccg cctacgtcct ggacgaggag gacttccgtt cgcgtgcccg
      961 cgacttccgc gacagcttct cggctgcctt cgagccgctc gcgggggccg acgtctacta
     1021 cgccggcaag gccttcctct gcacggcggt cgcccggtgg gtcgaggagg aggggctcag
     1081 tctcgatgtc tgcaccggag gcgagctggc cgtcgcccag cgggtgaagt tccccggcga
     1141 gcggatcggc ttccacggca acaacaagag cgtcgcggag atccgaacgg cactgaccta
     1201 cggggtgggc cgcatcgtcg tcgactcctt ccaggagatc gagcgcatcg cggcggtcgc
     1261 cgccgagctc ggggtggtcg cccccgtcat ggtccgggtc acggtcggcg tcgaggcgca
     1321 cacccacgag ttcatcgcca ccgcgcacga ggaccagaag ttcggcttct cgctgggtga
     1381 cggggcggcc tttgaagggg cccgccggat cgtcgcgcac ccggagcagc tgcgcttcct
     1441 cggtctacac agccacatcg ggtcgcagat cttcgacacc ggcggcttcg agaccgccgc
     1501 tgcccgcctc atcggtctgc atgcccggat cgccgaggag ttgggctgca acgccccgga
     1561 gatcgacctc ggcggcggct atggcattgc ctacaccagt cagcacacgc cgctgcctcc
     1621 tgagttgctc gcggagcaga tgagtcacat cgtgcgccat gagttcaagc ggatcgccga
     1681 tgtcgatccg gacggcgggc tgccgagggt ctcgatcgaa ccagggcgtg cgattgtcgg
     1741 cccggccgcc ttcacgctct acgaggtggg gaccgtcaag ccagtgaccg ttgccgatgg
     1801 ccaagtgcgc acctatgtct cggtcgacgg cgggatgagt gacaacccac ggccggcgct
     1861 ctacgaggcc gacttctccg caaccctcgc caatcgctcc tcgagtgcgc cacccgtggt
     1921 cgtccgcatc gtcggacggc actgcgaagc cggtgacatc gtcgtcttcg acgagtggct
     1981 gcccggtgac gtggcgccgg gggacctcat cgccgtcccc ggcaccggtg cctactgccg
     2041 ggcactgtcg agtcagtaca accacacccc ccgtccaccg gtcctcgccg tcacgtcgga
     2101 acgtgctcgg gtcatcgtgc gtcgtgagac catcgatgac ctgctcgcgc tcgatgtcga
     2161 cgagtagcca caaccgtcca gtcctcccgt ccccactgcc cccgcagcgc cgaacagacc
     2221 gcaccaggag cacagcactc atgtccgtgg ccgcccgccc acccctgcgc gtcgccctcc
     2281 tcggctgcgg cgtcgtcggc tctgccgtcg cccgactgct cgtcgagaac tccgctgacc
     2341 tcgcctcgcg gatcggggcg cccctcgagc tcgtggggat tgccgtccgg cgactggggc
     2401 gggatcgctc ggacgtgccg gtcgaccctg cactcttcac caccgacgct gaaggactgg
     2461 tggcccgagc cgacatcgtc atcgaggtcg tcggcggcat cgagccgacg cgggatctga
     2521 tcctgtccgc catccaccac ggcgcctcgg tcgtgagcgc caacaaggcc ctcctcgccg
     2581 aggacggacc cacgctctat gcggc
//
LOCUS       DAOSOC010000256         2528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_41108_length_2528_cov_8.512333, whole genome shotgun sequence.
ACCESSION   DAOSOC010000256 DAOSOC010000000
VERSION     DAOSOC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41108_length_2528_cov_8.512333"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..855)
                     /gene="pdxY"
                     /locus_tag="PL014_12690"
     CDS             complement(1..855)
                     /gene="pdxY"
                     /locus_tag="PL014_12690"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HOT57993.1"
                     /translation="MTTILSIQSSVAYGHVGNSAAVFPLQRLGVEVWPVHTVHFSNHT
                     GYGAWRGPLMAPEDVREVITGIEERGALPEVDAVLSGYQGGEQIGDVILDAVARTKAA
                     NPNAIYACDPVMGNAASGCFVHPAIPVLLREKVVPQADLITPNQFELGFLTDTEPGDL
                     DSTLESVERARAMGPGTVLVTSVLRPDRPEGTIEMLAVHGDGAWIVQTPQLPMKANGS
                     GDVTAALFTAHLLESGDAGVALGRTVSSVFDLLTRTHESGRRELQLVQSQDEIANPRM
                     QFEVRQLR"
     gene            1292..>2528
                     /locus_tag="PL014_12695"
     CDS             1292..>2528
                     /locus_tag="PL014_12695"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOT57994.1"
                     /translation="MTDRTSGIDPADVEQVRVLAQAARAASRRLALLSRADKDAALRT
                     LADALDAATDEIVAANELDLARGRENDMPQSLQDRLRLDPPRVAAVAQALRDIAALPD
                     PVGEVVRGSTLANGLQIRQIRVPMGVIGMIYEARPNVTVDAAGLGLKSGNAVILRGGS
                     AAAETNRATVAVLRASLTAQGLPADAVGLLEGGHGAVRALMTARGQVDLLIPRGGAGL
                     IQSVVTESTVPVIETGIGNCHVYVDARADLDKALAITVNSKTHRPSVCNAAESLLVHR
                     DVAAEFLPKALAALAGEGVVLHTDADAGAQADVAGVAHDVATDEHFATEFHGLEMSVR
                     VVDDLDQALEHIATYGSGHTEAIVTEDRGAARRFTAEVDAAAVMVNASTRFTDGGEFG
                     FGAEIGISTQKLHARGPMAL"
BASE COUNT          362 a          946 c          875 g          345 t
ORIGIN      
        1 tcagcggagc tgacggacct cgaactgcat ccgcgggttg gcgatctcgt cctggctctg
       61 cacgagctga agctcccgcc ggccggactc gtgcgtgcgc gtgaggaggt cgaacacact
      121 ggacacggtg cgcccgaggg cgaccccggc atccccgctc tcgagcaggt gcgcggtgaa
      181 cagtgcggcc gtgacgtcgc ccgagccgtt ggccttcatc ggcagctgcg gggtctgcac
      241 gatccaggcg ccgtcgccgt ggacggccag catctcgatg gtgccttcgg ggcggtcggg
      301 gcgcagcacg ctcgtgacga gcaccgtgcc cggccccatg gcccgcgccc gctcgaccga
      361 ctccagggtc gagtcgaggt cgccgggctc ggtgtcggtg aggaagccga gctcgaactg
      421 gttgggcgtg atgaggtcgg cctgcggcac gaccttctcg cgcagcagca ccgggatcgc
      481 cgggtgcacg aagcagccgg atgccgcgtt gcccatgacg gggtcgcacg cgtagatcgc
      541 gttcgggttg gcggccttgg tgcgggccac ggcgtcgagg atgacgtccc cgatctgctc
      601 gccaccctgg tagccggaga gcacggcgtc gacctccggc agggcgccgc gctcctcgat
      661 gccggtgatg acctcgcgga cgtcctcggg tgccatgagg gggccgcgcc acgcgccgta
      721 cccggtgtgg ttggagaagt gcaccgtgtg cacgggccac acctcgacac cgaggcgctg
      781 gagcggaaag actgcggctg agttgccgac gtggccgtac gccaccgagg actggatgga
      841 caggatcgtg gtcacccgcc catcctccca gaccgtgggc gtccctcgat cggccgatcg
      901 cgcgatgccg gggggcgggg ccggacgcca agctccgggg ccgattctgc atctgcacct
      961 tcggtgcgtg gcggccccaa cagggaccgc gatgacagac gtccggatgc ttcacacggt
     1021 gagccagccc ggctcacgac gcgatccatc ccaaagttgg ttcgtgcagc ggggtggctc
     1081 acgaggtgat ccatcccaaa gttggttcgt gcagcggggt ggctcgccac gtgaagcatc
     1141 cggactgccc gccgtggggt ctcccccaag ggcacaggat cgcaagcgcc cgaatctgtg
     1201 tcggcgtcga gccctcgagc cctcgagcgc ccgcaccccg agcgcagcca cctcggcgca
     1261 gccgaaccga gcgacgacct acccttgtgt catgactgac cgcacctccg gcatcgaccc
     1321 cgccgacgtc gagcaggtcc gcgtgctcgc gcaggccgcc cgggccgcct cgcgccgcct
     1381 ggccctgctc tcacgcgccg acaaggatgc cgcgctgcgc accctcgccg acgcgctcga
     1441 cgcagccacc gacgagatcg tcgccgccaa cgagctcgac ctcgcccgtg gccgagagaa
     1501 cgacatgccg cagagccttc aggaccgcct gcgcctggac cccccgcggg tcgccgccgt
     1561 ggcccaggcc ctgcgcgaca tcgcggccct gcccgacccc gtcggcgagg tcgtgcgcgg
     1621 ctcgaccctg gccaacggcc tgcagatccg ccagatccgg gtgcccatgg gcgtcatcgg
     1681 catgatctac gaggcccgcc ccaacgtcac cgtcgacgcg gccgggctcg gcctgaagtc
     1741 cggcaacgcc gtcatcctgc gcgggggcag cgccgccgcc gagaccaacc gcgccaccgt
     1801 cgccgtcctg cgggcctcac tcaccgccca ggggctgccc gcggatgccg tcggcctcct
     1861 cgagggtggc cacggcgccg tccgcgccct gatgaccgcc cgcggccagg tcgacctgct
     1921 catccctcgc ggcggggcgg gtctcatcca gagcgtcgtc accgagtcca ccgtgcccgt
     1981 catcgagacc ggcatcggca actgccacgt gtacgtcgac gcccgcgccg acctcgacaa
     2041 ggcgctggcg atcaccgtca actccaagac ccaccgcccg agcgtgtgca acgccgccga
     2101 gtcgctgctc gtgcaccgcg acgtcgccgc cgagttcctg cccaaggcgc tggcggcgct
     2161 cgcgggggag ggcgtcgtcc tccacaccga cgcggatgcc ggtgcgcagg ccgatgtcgc
     2221 gggcgtcgcc cacgacgtgg ccaccgacga gcacttcgcc accgagttcc acgggctcga
     2281 gatgtcggtg cgggtcgtcg acgacctcga ccaggcgctc gagcacatcg ccacctacgg
     2341 ctcgggccac accgaggcca tcgtcaccga ggaccggggg gccgcccgcc ggttcaccgc
     2401 ggaggtcgat gcggccgccg tcatggtcaa cgcgagcacg cggttcaccg acggtggtga
     2461 gttcggcttc ggggccgaga tcggcatctc gacccagaag ctgcacgccc gcgggccgat
     2521 ggcgctgc
//
LOCUS       DAOSOC010000257         2520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_41264_length_2520_cov_6.344422, whole genome shotgun sequence.
ACCESSION   DAOSOC010000257 DAOSOC010000000
VERSION     DAOSOC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41264_length_2520_cov_6.344422"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1543
                     /locus_tag="PL014_12700"
     CDS             83..1543
                     /locus_tag="PL014_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT57995.1"
                     /translation="MNDYVDTPRGYLGHVGSVTVVTHNVLTSGLLPKNMGWSDDGYRD
                     TTGLLPGTNPFWIPSSWSRQDMFTVQESIGKRRLPDYLKAADPTAKTFAVSPKGYAAW
                     AFGGPTADSIITFGSNTSCGDTNRRGPSGVNVPAYIAQPCGRYYVKRSSSLTYDTHQW
                     PASLYPLDGNRYTTGKEDDHLGGDIWATDIALDIMDNENWNGIFLTLPDVDKNAHMWG
                     GVDDDEGAGLLDPDPMTHMKFAAKTADEQVGRVMDKLRADGELDDTLVVLTADHGSVP
                     GRDFLGTQNPELNYGYYNWYYGEAANDAPYLKPQDALKPLTDTDNVGLSYSDSMLRAW
                     LKDTSPAKVSEAASIMAGMPGVSAVWTRAGDRYTRVSPVRYDRMTNPGEKAWFVQHAQ
                     ELVDTEAAAYGPEVIATLVDDTTYSVAGDHGGIQRRSQQIPIVFAGADLSRTDLQAPV
                     RSVDVMPMLLKHMGIAVDPGLDGVGYTLPTRPSRGR"
     gene            complement(1549..2073)
                     /locus_tag="PL014_12705"
     CDS             complement(1549..2073)
                     /locus_tag="PL014_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOT57996.1"
                     /translation="MSAGVAPLVDAVPAALVALTLVGGAYLLHLGVATLRSTRTVGAP
                     NLSPTSDAVSPTPVSPTPVSPTTAGHGFLRRGIAVSALNPKSLLFFLAFLPQFTRQSA
                     PWPMPVQLLVLGVVWVVLAGLVYAAMGFTVQRTLAQRPHLQRGVALVAGVAMILAGVG
                     LLGEQLVHTLTAVA"
     gene            2101..2436
                     /locus_tag="PL014_12710"
     CDS             2101..2436
                     /locus_tag="PL014_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT57997.1"
                     /translation="MRRLEERGVILGYSARIDPAAVGRQFVVNVDFELAGQGKGLLQE
                     FEQALASYDEVVQCRRMLGRPDYQALVAVADLETYERFMTEELMALPNLGRLESRFVM
                     KTVKSDLTV"
BASE COUNT          443 a          874 c          869 g          334 t
ORIGIN      
        1 atcgcccgaa gcctgctcga gaccggcggc ttcagacccc aactcctaca cccccgattg
       61 ggatgagccc gtcaaggccc tgatgaacga ctacgtcgac acgccacgcg gatacctggg
      121 tcacgtcggg tcggtcaccg tcgtcactca caacgtgctg acgtccggcc tgctgcccaa
      181 gaacatgggc tggagcgacg acgggtaccg ggacaccacc gggctccttc caggcaccaa
      241 cccgttctgg atcccgagca gctggtcgcg ccaggacatg ttcaccgtgc aggagtccat
      301 cggcaagcgt cgcctgcccg actacctcaa ggccgcggac cccacggcga agaccttcgc
      361 cgtcagcccc aagggctacg cggcgtgggc gttcggtggg ccgaccgccg actcgatcat
      421 cacgttcggg tcgaacacca gctgcggcga cacgaaccgt cgcggtccgt ccggtgtcaa
      481 cgtgcccgcc tacatcgcgc agccgtgcgg ccggtactac gtcaagcgct cctccagcct
      541 cacctacgac acccaccagt ggccggccag cctctacccg ctcgacggca accggtacac
      601 caccggcaag gaggacgacc acctcggcgg tgacatctgg gccaccgaca tcgcgttgga
      661 catcatggac aacgagaact ggaacggcat cttcctcacg ctgcccgacg tcgacaagaa
      721 cgcacacatg tggggcggag tcgatgacga cgagggtgcc gggctgctcg accccgaccc
      781 gatgacgcac atgaagttcg ccgcgaagac cgcggacgag caggtgggcc gggtcatgga
      841 caagctgcgg gccgacggcg agctcgacga cacgctcgtc gtgctgaccg ccgaccacgg
      901 ctccgttccg gggcgcgact tcctgggtac gcagaacccc gaactgaact acgggtacta
      961 caactggtac tacggcgagg ccgccaacga cgccccgtac ctgaagccgc aggacgcgct
     1021 caagcccctc accgacaccg acaacgtggg cctgagctac agcgactcga tgctgcgggc
     1081 ctggctgaag gacacctccc ccgcgaaggt gagcgaggcg gcatccatca tggcggggat
     1141 gccgggtgtc tcggccgtgt ggacccgtgc cggcgaccgc tacacccggg tctcccccgt
     1201 gcggtacgac cggatgacca accccgggga gaaggcatgg ttcgtccagc acgcgcagga
     1261 gctcgtcgac accgaggccg cggcctacgg gcccgaggtc atcgccaccc tggtcgacga
     1321 cacgacgtac tcggtggccg gtgaccacgg cgggatccag cgtcgcagcc agcagatccc
     1381 gatcgtcttc gccggggccg acctgtcacg caccgacctg caggcgccgg tgcgctcggt
     1441 cgacgtcatg ccgatgctgc tcaagcacat gggcatcgcc gtcgacccgg ggctggacgg
     1501 cgtcggctac accctcccga cccgcccgtc ccggggccgc tgagggcgtc aggcaacggc
     1561 ggtcagtgtg tgcacgagtt gctcaccgag gagcccgaca cccgcgagga tcatcgcgac
     1621 cccggcgacc agggcgacgc cgcgttgcag gtgcggccgc tgggccaggg tgcgctgcac
     1681 cgtgaaaccc atcgccgcgt agaccagacc ggccagcacg acccagacca caccgagcac
     1741 gaggagctgc acgggcatcg gccacggcgc gctctggcgg gtgaactgcg gcaggaacgc
     1801 caggaagaag agcagcgact tggggttgag ggcactgacg gcgatccccc gccgcaggaa
     1861 cccgtgcccc gcggtggtgg ggctcacggg ggtggggctc acgggggtgg ggctcacggc
     1921 atccgacgtg ggtgaaaggt tcggggcgcc cacggtgcgg gtgctgcgca gcgtcgcgac
     1981 gccgaggtgg agcaggtagg caccgcccac gagggtgagg gcgacgaggg cggcgggaac
     2041 ggcatccacg aggggggcga ccccggccga gatgaccgcg gttcgccgtg cctgcggcgg
     2101 gtgcggcgcc tcgaggagcg cggcgtcatc ctcgggtact cagcacgcat cgaccccgcg
     2161 gcggtggggc ggcagttcgt cgtgaacgtc gacttcgagc tggccggaca gggcaagggg
     2221 ttgctccagg agttcgagca ggccctggcc tcgtacgacg aggtcgtgca gtgccggcgc
     2281 atgttagggc gccccgacta ccaggccctc gtcgccgtgg ccgacctcga gacctacgag
     2341 aggttcatga ccgaggagct catggcgctg ccgaacctcg ggcggctcga gtcgcggttc
     2401 gtcatgaaga cggtgaagtc ggacctcacc gtctgaaccc ggatgccggt gcttcccggg
     2461 cggactgtca gcggcgcacg actctgagac aggcgcctgc ctgggcggtc gataatgaga
//
LOCUS       DAOSOC010000258         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_42044_length_2486_cov_10.754833, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000258 DAOSOC010000000
VERSION     DAOSOC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42044_length_2486_cov_10.754833"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PL014_12715"
     CDS             complement(<1..1279)
                     /locus_tag="PL014_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOT57998.1"
                     /translation="MSTPLMPLAEAAETVDYAATAFLILCASLVLLMTIPGLALFYGG
                     MTRTKSVLNMMMMSFGAMGVVGIIYVLYGYSMSFGSENVGGIIANPFEKFGLAGTDTI
                     INPFGYEGYGNIPELAFVGFQLTFAVITVALISGAVADRVKFSTWMVFAALWVTFAYF
                     PIAHMVWGGGLLSGAEGGLAAAVFGVTDGVANVVPIDFAGGTVVHINAGVAGLVLALV
                     VGQRVGFGKVAMRPHNVPLTMIGAGLLWFGWFGFNAGSELAADETAALVWVNTTAATC
                     AAMLGWLVVEKMRDGHATSVGAASGVVAGLVAITPACGALSPLGSIILGVVAGGLAAL
                     AVGLKFKLGYDDSLDVVGVHLVAGLWGTVGAGLLATSTGLFYGGGIDQLVLQVLVALA
                     TLVISGVVTLIIALALKATMGWRVPEDVEIAGID"
     gene            1426..1845
                     /locus_tag="PL014_12720"
     CDS             1426..1845
                     /locus_tag="PL014_12720"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HOT57999.1"
                     /translation="MAPHPLTAAPLPVLVFDGDCAFCTTSAGVITRRLRRSPDDYAVQ
                     PWQHLDLDALGLTPEECDEAVRWVGENGIRDAGHVAIARALRASRWWVRPVGVVLVAP
                     GVNALAARAYRWVAANRHRLPGGTPACSLPSPPPPAA"
     gene            complement(1842..>2486)
                     /gene="ftsY"
                     /locus_tag="PL014_12725"
     CDS             complement(1842..>2486)
                     /gene="ftsY"
                     /locus_tag="PL014_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT58000.1"
                     /translation="MDRSIVSSRVDGRPACVLVVGVNGTGKTTTVGKLARVLVAQDKD
                     VVLGAADTFRAAAADQLQTWGERVGVPTIRSDREGADPAAVAFDAVKAAAEMEADVVL
                     IDTAGRLQNKVGLMDELGKVKRVIEKQSPVDEVLLVLDATTGQNGMRQAEVFSQVVGI
                     TGIVLTKLDGTAKGGIVVAVQRGLGVPVKLVGLGEGPDDLAPFEPEAFVDALLD"
BASE COUNT          400 a          855 c          878 g          353 t
ORIGIN      
        1 cgtcgatgcc tgcgatctcg acgtcctccg gaacgcgcca gcccatcgtc gccttgaggg
       61 ccagggcgat gatcagcgtg acgacacccg agatgacgag cgtggcaagc gcgacgagca
      121 cctgcaggac gagctggtcg ataccgccac cgtagaacag acccgtggag gtcgcgagca
      181 gtcccgcgcc gacggtgccc cagaggccgg cgacgaggtg gacgccgacg acgtcgagcg
      241 agtcgtcgta gccgagcttg aacttcaggc cgacggcgag tgcggccagg ccaccggcca
      301 cgacaccgag gatgatcgag ccgagcgggc tgagggcgcc acaggccggg gtgatcgcga
      361 cgagaccggc gacgacaccc gaggccgcac cgacggaggt ggcgtgaccg tcacgcatct
      421 tctccacgac gagccagccg agcatcgccg cgcacgtcgc ggcggtggtg ttgacccaca
      481 cgagggcggc ggtctcgtca gcggcgagct cggagcccgc gttgaagccg aaccagccga
      541 accacagcag gccggcgccg atcatggtga gcggcacgtt gtgggggcgc atggccacct
      601 tgccgaagcc gacgcgctgg ccgacgacga gcgccaggac cagcccggcg acaccggcgt
      661 tgatgtgcac gaccgtgcca ccggcgaagt cgatcggcac gacgttggcg acgccgtcgg
      721 tcacgccgaa cacggccgcg gccaggcctc cctcggcacc gctgagcagg ccgccgcccc
      781 acaccatgtg ggcgatcggg aagtacgcga aggtgaccca gagggcggcg aagaccatcc
      841 aggtggagaa cttcacccgg tcggcgacgg cgccgctgat gagggcgacg gtgatgaccg
      901 cgaaggtcag ctggaagccg acgaacgcga gctcggggat gttgccgtag ccctcgtagc
      961 cgaaggggtt gatgatcgtg tcggtgccgg cgaggccgaa cttctcgaac gggttcgcga
     1021 tgatcccgcc gacgttctcg ctgccgaagc tcatcgagta gccgtacagg acgtagatga
     1081 tgccgacgac gcccatcgcg ccgaacgaca tcatcatcat gttgaggacg gacttggttc
     1141 gggtcatgcc gccgtagaac agggcgagac ccgggatggt catgaggagg acgagggagg
     1201 cgcagaggat caagaacgcc gttgctgcgt agtcgaccgt ctccgctgcc tccgccaggg
     1261 gcatcagtgg tgtgctcatg ccgtcaacga tcaccccggt ccgttacccc tcactccccc
     1321 ggatgtttcg gggatgttgc atcaggccag tgggtgtaag catccggttt cacaggacca
     1381 gaggtctcgc atcctgagac ctggtgaccg tgggacgata cggccatggc tccacacccg
     1441 ctcaccgccg cacctctgcc ggtcctggtg ttcgacgggg actgcgcgtt ctgcacgacc
     1501 tcggccggcg tcatcacgcg ccggctgcgg cgctcccccg atgactacgc cgtgcagccg
     1561 tggcagcacc tcgatctcga cgccctgggc ctgacgcccg aggagtgcga cgaggcggtg
     1621 cgctgggtcg gtgagaacgg catccgggat gccggtcacg tcgccatcgc gcgggcgctg
     1681 cgcgcgtcac gctggtgggt gcgcccggtc ggtgtggtgc tggtggcgcc gggggtcaac
     1741 gccctcgcgg cccgtgccta ccggtgggtc gcggcgaacc ggcaccgcct gcccggcggc
     1801 acccccgcct gctccctccc gtcgcccccg cctccggcgg cctagtccaa cagggcgtcg
     1861 acgaacgcct cgggctcgaa cggcgccagg tcgtcgggac cctcgccgag cccgacgagc
     1921 ttgaccggca cacccagacc gcgctggacg gcgacgacga tgccgccctt ggcggtcccg
     1981 tcgagcttgg tgagcacgat gccggtgatg ccgaccacct gcgagaagac ctcggcctgg
     2041 cgcatcccgt tctggcctgt tgtggcgtcg aggacgagca ggacctcgtc gaccgggctc
     2101 tgcttctcga tgacccgctt gaccttgccg agctcgtcca tgaggccgac cttgttctgg
     2161 aggcgcccgg cggtgtcgat gagcacgacg tcggcctcca tctcggcagc ggccttgacg
     2221 gcgtcgaagg ccaccgcggc ggggtcggcg ccctcgcggt cggagcggat cgtcggcacc
     2281 ccgacgcgct cgccccaggt ctggagctgg tcggcggctg ctgcacggaa ggtgtccgcg
     2341 gcaccgagga cgacgtcctt gtcctgcgcg acgaggacac gggcgagctt gcccacggtc
     2401 gtggtcttgc cggtgccatt gaccccgacc acgagcacgc acgccggtcg gccgtcgacc
     2461 cgcgacgaca cgatcgagcg gtccat
//
LOCUS       DAOSOC010000259         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_43558_length_2420_cov_11.019027, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000259 DAOSOC010000000
VERSION     DAOSOC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43558_length_2420_cov_11.019027"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PL014_12730"
     CDS             <1..1080
                     /locus_tag="PL014_12730"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT58001.1"
                     /translation="VIGRALGGDSATAAVISTVIVMIALLPARSLLLRVLGRRLHPRR
                     ERGVEAVRSLVTAVHAGDDQPERLEQVLRDGLRDPGLRVGYQRPGSTTFVDVDQSPVP
                     VQGSTPITLAGNVVGVIRPGDGGAGIPDEVAREAGLLAESVRLRGELSEALAAVEASR
                     RRLLSAGYEERRRLEMDLHDGAQQRLVSLGMRLRVAQHRAAGGDGPDLDALVDGAVDE
                     LSLAVAELRQIAHGLRPSCLDDGLAPALESLTRSSALPLEVTYSADDLPDHVSLTAYY
                     VASEGVANAVKHAAASRVAVDVRQEESVVHVAVRDDGRGGARLTPGSGLAMLSDRVNA
                     LGGRLTVSSEPRGGTSIEAVIPCAS"
     gene            1068..1715
                     /locus_tag="PL014_12735"
     CDS             1068..1715
                     /locus_tag="PL014_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT58002.1"
                     /translation="MRIVIGEDSALFRAGLRSLLTEVGHDVVADAPDAPSALAAVLEH
                     GPDVAILDVRMPPDHADDGARVAARVRLERPETGVLLLSQHVESRLSVDLVAGGHFGY
                     LLKDRVLDVTQFLDALDRVADGGSALDPEVVARLMVGGSPGLASLTPREHEVLALMAE
                     GLTNAGIAARVVIAERTVETHVARILGKLGLAEATTQDHRRVLAVLRYLEGHRPG"
     gene            1879..2169
                     /locus_tag="PL014_12740"
     CDS             1879..2169
                     /locus_tag="PL014_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOT58003.1"
                     /translation="MSETTRLPGPVADLWDWQLEGSCRRVGPDVFFHPEGERGTKRRI
                     RAGAAKEVCFGCPVIDACRSHALAVREPYGVWGGLTEDERESFYARGRHIAS"
     gene            2327..>2420
                     /locus_tag="PL014_12745"
     CDS             2327..>2420
                     /locus_tag="PL014_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOT58004.1"
                     /translation="MSAPIDVPIRDETIRLGQLLKLAGVVDDGAM"
BASE COUNT          330 a          870 c          863 g          357 t
ORIGIN      
        1 gtgatcggac gcgccctcgg cggtgactcg gcaaccgctg ccgtgatctc gacggtcatc
       61 gtcatgatcg cgctcctccc ggcccggtcc ttgctgttgc gggtgctcgg ccgccgcctg
      121 cacccgcggc gcgagcgagg cgtcgaggcg gtccgttcgc tcgtgacggc cgtgcacgcg
      181 ggcgacgacc agcccgagcg gctcgagcag gtgctgcgcg acgggctgcg cgaccccggg
      241 ctgcgggtcg ggtaccagcg gccgggcagc acgaccttcg tcgacgtcga ccagagcccc
      301 gttccggtac agggcagcac cccgatcacg ttggcgggca acgtcgtcgg ggtgatccgc
      361 cccggggacg ggggtgccgg catccccgac gaggtcgcgc gtgaggccgg cctgctggcc
      421 gagtcggtgc gccttcgtgg agagctctcc gaggccctgg ccgcggtcga ggccagccgt
      481 cggcgcctgc tcagcgccgg gtacgaggag cgccgccggc tcgagatgga cctgcacgac
      541 ggcgcccagc agcggctggt ctccctcggc atgcgcctac gggtggccca gcaccgggca
      601 gccggaggtg acggcccgga cctcgacgcc ctggtcgacg gggcggtcga cgagttgtcc
      661 ctcgccgtgg ccgagctgcg ccagatcgcc cacgggctgc gcccgagctg tctcgacgac
      721 gggttggctc cggccctgga gagcctgacc cgctccagcg ccctgcccct cgaggtcacc
      781 tactcggccg acgacctgcc cgaccacgtg agcctcaccg cctactacgt cgcgagcgaa
      841 ggcgtcgcca acgccgtcaa gcacgccgcg gcatcccgcg tcgccgtcga cgtgcggcag
      901 gaggagtccg tcgtccacgt cgccgtgcgc gacgacgggc gcggtggcgc ccgcctcacg
      961 cccggttccg ggctcgccat gctgagcgac cgcgtgaacg ccctgggcgg tcggctgacc
     1021 gtgtcctccg agccgcgcgg cggaacctcg atcgaggcgg tcatcccttg cgcatcgtga
     1081 tcggcgagga ctcggcgctc tttcgtgccg ggttgcggtc gctgctcacc gaggtgggcc
     1141 acgacgtcgt cgccgacgcc cccgacgccc cgagcgcgct ggccgcggtg ctcgagcacg
     1201 gccccgatgt cgcgatcctc gatgtccgga tgccgcccga ccacgccgac gacggcgccc
     1261 gggtcgcggc ccgggtccgg ctcgagcgac cggagaccgg ggtgctgctg ctctcgcagc
     1321 acgtggagtc acgactgtcg gtcgacctcg tggccggtgg ccacttcggc tacctgctca
     1381 aggaccgggt tctcgatgtc acgcagttcc tcgacgccct cgaccgggtc gccgacggcg
     1441 gttcggcgct cgacccggag gtggtggctc ggctcatggt cggcggcagc cctgggctgg
     1501 cctcgctcac cccgcgcgag cacgaggtgc tggccctgat ggccgagggg ttgacgaacg
     1561 cggggatcgc cgcccgggtg gtcatcgccg agcgcaccgt cgagacccac gtcgctcgga
     1621 tcctcggcaa gctgggcctg gcggaggcga ccacgcagga ccatcgccgg gtgctcgcgg
     1681 tgctgcggta cctcgagggg caccgacctg gttgagagct gaaaaacgtt ccgtcgcagg
     1741 ctagttgacg tctgaatcac atagctgcag tttttgacca actccttacc tttgacatac
     1801 ccgacctttt ccaaaccact cctagcgtgg tgcaccggcc actcagggaa gtggtcgaca
     1861 ccacgtgagg aggtaggcat gtcggagacc accaggcttc cggggcccgt tgccgacctg
     1921 tgggactggc agctcgaggg gtcctgccgg cgagtcgggc ccgacgtgtt cttccacccc
     1981 gagggcgagc gcggcaccaa gcgccgcatc cgggcaggcg cagccaaaga ggtgtgcttc
     2041 ggctgtccgg tgatcgatgc ctgccgcagc cacgcactcg ccgtgcgcga gccgtacggg
     2101 gtgtggggcg gcctcacgga agacgagcgc gagtccttct atgcacgggg ccgtcacatc
     2161 gcgtcctgac gtcaccttcg tggccaggac tccaccagac cgggggcgag aagcagtccg
     2221 ggccgcaggg gccgcggggg ccgcaacgac agatcacacc tgtcgagacg agaacccacc
     2281 tgcggcccgg acaacgtcgc acggcacgtc gccgtagcct tgatgcgtga gtgctcccat
     2341 cgacgtcccc atccgtgacg agacaatccg cctcgggcag ctgctcaagc tggcgggcgt
     2401 cgtcgatgac ggcgccatgg
//
LOCUS       DAOSOC010000260         2341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_45612_length_2341_cov_6.137358, whole genome shotgun sequence.
ACCESSION   DAOSOC010000260 DAOSOC010000000
VERSION     DAOSOC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45612_length_2341_cov_6.137358"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..1654)
                     /locus_tag="PL014_12750"
     CDS             complement(281..1654)
                     /locus_tag="PL014_12750"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOT58005.1"
                     /translation="MSIDTTDLSSDPVADRVVRVQVGIDAAVVANHQVTIRETTRDGQ
                     VQLSRFHASPTIAGLTRLSERLAAHPHVMAVAEPTSMTWLGLHVALQEAGCDLALVGT
                     RHAARLRGAISGKNKSDVIDADVLAMAGDVFTLSPLRPPAPPQLALRRAVVRRGKLVI
                     DGNRSRRRLISLARWAFPDVWQEFAGSWPTAVAVLTRWPDLTALASARRSSVTNVVAA
                     HTRGVPDVDARAERIRAAAARTTAFWDGHLDLEALAWETSEHLRDIALAAEQIERATD
                     QAHHYWAALYGDDPLLLSVPGMGPMTAPTIRAFFADASAFPTAKAAAAYVGLTPSTWS
                     SGTVVQPSRAITKEGPAVLRLACYQAAGGARRVDPQLAEYYRHLMVERGHCHTQATVA
                     VARKLVERTWTVLTRGQPFEYRDLDGTPLTRAAAKDAARALAVPKTVRARARARSAAT
                     HRSKLTK"
BASE COUNT          385 a          827 c          786 g          343 t
ORIGIN      
        1 caacgccacg atcacacctg cggcggctgc ttgacataca taggagattc aacggccgcc
       61 tggaagccct acgccgcaac gccctcggat tccgcaacct cacccactac cgaatccgct
      121 cactgctgca ttgcggcaac ctcgcccacc agatcgacgc actctaaatc cggaagagat
      181 tccctaacgg ttgtcaagcc gtagggggta ggtcgttggt ggcatgacga gatacccgga
      241 ccaccgctgc cctggtggcc gggtcggggc gttccttgta ctacttggtc agcttggagc
      301 ggtgcgtggc cgcgctgcgc gctcgagctc gggcccggac ggtcttgggg acggccaggg
      361 cccgggccgc gtctttggct gctgctcggg tcaggggagt gccgtcgagg tcgcggtatt
      421 cgaagggttg gcctcgggtc aggacggtcc aggtccgctc gacgagcttg cgggcgacgg
      481 cgaccgtggc ctgggtgtgg cagtgtccac gctcgaccat gaggtggcgg tagtactccg
      541 cgagctgggg atcgacccgg cgggccccgc cagcggcctg atagcacgcc aaccgcagca
      601 ccgctggacc ttccttcgtg attgctcggg agggctggac cacggtcccg gacgaccacg
      661 tcgacggggt cagcccgacg taggccgcag cggccttggc ggtggggaac gcggacgcgt
      721 cggcgaagaa cgcccggatc gttggcgctg tcatcgggcc catccccggc accgacagca
      781 gcagcgggtc gtctccgtac agggccgccc agtagtggtg ggcctggtcg gtggcccgtt
      841 cgatctgctc cgccgcgagg gcgatgtcgc gcaggtgctc gctggtctcc caggccaaag
      901 cctccaggtc gaggtgcccg tcccagaagg cggtggtgcg ggcggcggcg gcccggatcc
      961 tctcggcgcg cgcgtcgacg tcgggcacgc cgcgagtgtg agcggcgacg acgttggtca
     1021 cgcttgagcg tcgagccgag gccaacgcgg tcaggtcggg ccaccgagtc aggacggcga
     1081 ccgcggtcgg ccaggacccc gcgaactcct gccacacatc gggaaacgcc caccgggcca
     1141 acgagatcag ccgtcggcgg gaacggttgc cgtcgatgac gagcttgccc cgacggacga
     1201 ccgcacgacg cagtgccagc tgcggcgggg cgggtgggcg cagcggggac aaggtgaaca
     1261 catcgccggc catggcaagg acgtcggcgt cgatgacgtc ggacttgttc ttgcccgaga
     1321 tcgctccccg caggcgggca gcatgccgcg tcccgaccaa ggccaagtcg cacccggcct
     1381 cctgcaaggc cacgtgcagg cccagccagg tcatcgaggt cggctccgcg accgccatga
     1441 cgtgcgggtg ggctgccaac cgttcactca ggcgggtcaa cccggcgata gtcggtgagg
     1501 cgtggaaccg cgacagctgg acctgtccgt cgcgggtggt ctcgcggatc gtgacctggt
     1561 gattggcgac cacggcggcg tcgatcccga cttgcacgcg cacgacccga tcagcgacag
     1621 ggtcgctgct gaggtcggta gtgtcgatgg acacgagggt tcctccattc gttgcgaacg
     1681 gacgactccc ggcccgtact ctcaccggcc agtgaccacg cgggacgggg ttcgtcatga
     1741 cgggtgggag aaccctcgtc cagcccgcca gcacccttgc tccagatcag ctcggcaccg
     1801 gatcgacctg cacgtggagg cactcccgga cctccgccac ccagccaccg cagcagccgc
     1861 cacggtaggc aaacccacgc ttgtgcgtga tccggcgcct tcaggacgac gggcaccgcg
     1921 ccgcgggcca ccgacctgcc accaattccc tgaaccgggt tcgacctcga ccgggagagc
     1981 cgaccccggg cccaccgatc tctttcacga gccacaacgc tcacccggaa accggcacga
     2041 cccgaggcca tctcccacgg ccaccgtagc gaccaccacc tgtcgccagg aggcccttca
     2101 agtcatacaa gcccctttat tcgagcgcgc tcccccaaaa ccatcgaaga ctcgagcctc
     2161 cgacgctggg catgacagcc gccgagaagt cgcgacggcg tgaagcgttc gatgcccgcg
     2221 agccctaccg tttcgtgccc catgccaagc gcgacgctat ccgcgggacg cagcgcttcc
     2281 gtggcccagc ggtcgacggg caccccttca aggtccgctg ccccaacacg cccgcctcca
     2341 t
//
LOCUS       DAOSOC010000261         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_46041_length_2325_cov_9.207930, whole genome shotgun sequence.
ACCESSION   DAOSOC010000261 DAOSOC010000000
VERSION     DAOSOC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46041_length_2325_cov_9.207930"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PL014_12755"
     CDS             <1..327
                     /locus_tag="PL014_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOT58006.1"
                     /translation="ESEQQFAISRIRAIEHGRSVAHVSTVGVSGFIAPDGSVSGKTGL
                     FTAEQEIGVPVVRDELSPSDRLGPVPEWLAAALLLALVVGSVRSRRSVRVLAPSIRPR
                     EDHTVV"
     gene            320..1105
                     /locus_tag="PL014_12760"
     CDS             320..1105
                     /locus_tag="PL014_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOT58007.1"
                     /translation="MSETTRPPIQRVAVLIPTYNERDTLPVIVARLREVVPDADLVVL
                     DDNSPDGTGAVADALAAADPRVQVLHRAGKEGLGAAYLAGFEWALERGYDAIVEIDAD
                     GSHRPEHLPALLAAAADADAVIGSRWVPGGAVVNWPLHRKALSLGGNIYVKVLLGMPV
                     NDATAGFRVYRADALRVMGLHDVASQGYCFQTDLTWRAVRSGLRVVEVPITFVEREVG
                     DSKMSRAVMTESLRRITGWGVRHRATQVRDLVGGARQPRWHSL"
     gene            1102..1641
                     /locus_tag="PL014_12765"
     CDS             1102..1641
                     /locus_tag="PL014_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HOT58008.1"
                     /translation="MSRPGATPYAARGRRRPRVVRWVFLALLIIPIVEIAAIIAVGRV
                     IGGWPTLALLVLESMLGAWIVKREGARTWAALQEALTTGQMPSRQLADAALVLIGGTL
                     LLTPGFVTDIVGFFFILPLTRPITRAWLQAVVAGRLLGPLGEWPGGPGPSATPRSGRP
                     DVVEGEVVQGRIVDDESTP"
     gene            complement(1743..2105)
                     /locus_tag="PL014_12770"
     CDS             complement(1743..2105)
                     /locus_tag="PL014_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HOT58009.1"
                     /translation="MAERSLRGSRLGALSMETDHNVVPSERNITTYLCPNGHSIELPF
                     SVEADVPHTWECRCGEDAKLQGGGPPPELKPVKHVRTHWDMLLERRSIPELEELLEER
                     LTLLRESRGERPRKRKSA"
BASE COUNT          299 a          817 c          828 g          381 t
ORIGIN      
        1 gagtccgagc agcagttcgc gatctcgcgc atccgcgcga tcgagcacgg ccggtcggtg
       61 gcccacgtgt ccaccgtggg ggtctccggg ttcatcgccc ccgacgggtc ggtgtcggga
      121 aagaccgggc tcttcacggc ggagcaggag atcggggtgc cggtggtgcg cgacgagctc
      181 tcaccctcgg atcggctggg cccggtgccg gaatggctcg ccgctgccct gctgctcgcg
      241 ctcgtcgtgg ggagcgtgcg cagccgccgg agcgttaggg ttctcgcacc gtcgatccga
      301 ccccgcgagg accacaccgt tgtctgagac cacccgtccg ccgatccagc gtgtcgctgt
      361 cctgatcccg acgtacaacg agcgcgacac cctgcccgtc atcgtggccc gcctgcgcga
      421 ggtggtgccc gacgcggatc tggtcgtgct cgatgacaac tcgcccgacg gcaccggggc
      481 ggtggccgac gccctcgcag cggccgaccc gcgcgtccag gtcctgcacc gggccggcaa
      541 ggagggcctc ggagcggcct acctcgctgg cttcgagtgg gccctggagc gcggctacga
      601 cgccatcgtg gagatcgacg cggacggctc acaccggccc gagcacctgc cggccctgct
      661 cgcggcggca gccgacgcgg atgccgtgat cggctcacgc tgggtgcccg gtggcgccgt
      721 cgtcaactgg ccccttcacc gcaaggcgct ctcgctcggc ggcaacatct acgtcaaggt
      781 gctgctcggc atgcccgtca acgacgcgac ggccggcttt cgggtctacc gggccgacgc
      841 cctgcgggtc atggggctgc acgacgtcgc ctcgcagggc tactgcttcc agaccgacct
      901 gacctggcgc gcggtgcgca gcgggctgcg cgtcgtcgag gtccccatca cgttcgtcga
      961 gcgtgaggtc ggggactcca agatgagtcg tgccgtcatg accgagtcgc tgcggcggat
     1021 caccgggtgg ggagtacgcc accgcgccac tcaggtgcgc gacctcgtgg gcggggcgcg
     1081 ccagccgagg tggcactcac tgtgagcagg ccgggcgcga caccgtacgc cgcccgcggt
     1141 cgacggcgcc cccgggtggt gcgctgggtc ttcctcgccc tgctgatcat ccccatcgtc
     1201 gagatcgccg cgatcatcgc cgtgggccgc gtcatcggcg gctggcccac gctggcgctc
     1261 ctcgtcctcg agtcgatgct cggagcgtgg atcgtcaagc gtgagggcgc acgcacctgg
     1321 gcagcccttc aggaggccct gaccacggga cagatgccca gtcgacagct cgccgatgcc
     1381 gcgctcgtgc tgatcggcgg caccctgctg ctcacaccgg gtttcgtcac ggacatcgtg
     1441 ggcttcttct tcatcctgcc gctgacccgc ccgatcaccc gggcctggtt gcaggcggtc
     1501 gtcgccgggc ggctgctcgg accgttgggg gagtggcccg gtggtcctgg cccgtcagcg
     1561 acgccccgga gtggtcgccc ggacgtcgtc gagggggagg tcgttcaggg gcgcatcgtc
     1621 gacgacgaga gcaccccctg aacctggccc acgcggctct ctgcgaggca ccccacacgc
     1681 gaggatgccg ggcaccccgc tgggggtgcc cggcatccgg gtcgtgagct ctgccgctgc
     1741 ggtcaggcgg acttgcgctt gcgagggcgc tcaccgcgcg actcgcgcag cagggtcagg
     1801 cgctcctcga ggagctcctc gagctcgggg atggagcgcc gctcgagcag catgtcccag
     1861 tgcgtacgca cgtgcttgac gggcttgagc tccggcggcg ggccaccgcc ctggagcttt
     1921 gcgtcttccc cgcagcggca ctcccaggtg tgcggcacat cggcctcgac cgagaacgga
     1981 agctcgatgg agtgcccgtt ggggcacagg taggtggtga tgttgcgctc gctggggacg
     2041 acgttgtggt cggtctccat gctcagggcg ccgagacggc tgccgcgcag gctacgttct
     2101 gccatgggta actcccgagt ctgtggtgtc ccgatcggca gttcgccccg accgggtcta
     2161 gcgcatccaa cgcccggata ccggtcgttg ttccgccgtg agcaggtgct gtcgaaggtt
     2221 cgacgtgacc gcactcacag tctcgcgtga tccttcggct gaacggacgc accgtgcccg
     2281 acaggtgggt acgggtgctc aattcccgtg ttgtacgttc ccccg
//
LOCUS       DAOSOC010000262         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_46925_length_2293_cov_12.172029, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000262 DAOSOC010000000
VERSION     DAOSOC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46925_length_2293_cov_12.172029"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..>2293)
                     /gene="acnA"
                     /locus_tag="PL014_12775"
     CDS             complement(234..>2293)
                     /gene="acnA"
                     /locus_tag="PL014_12775"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOT58010.1"
                     /translation="MVNGLGVLGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGAARA
                     GVTATDVVLTITEQLRQHGVVGKFVEFYGEGVAALPLANRATIGNMSPEFGSTCAIFP
                     IDDITLDYLRLTGRDEEQVALVEAYAKEQGMWLDPAAEPRFSEKLELDLSTVVPSIAG
                     PKRPQDRIALSDSKQQFALDVKNYGVDGELRSVPVQAGGEDTGRGGTSLFDLKDGAVV
                     IASITSCTNTSNPSVMMAAALLAKNAVERGLTVKPWVKTSMAPGSKVVTGYYEAAGLW
                     PYLDKLGFNLVGYGCATCIGNSGPLPAYVSSAIQAEDLSVAAVLSGNRNFEGRINPDV
                     KMNYLASPPLCIAYALAGTMDFDFDTDPLGQDEAGNDVFLADIWPAPQEVEAVIASSI
                     TRDLFTADYADVFAGDERWQSLPTPEGDTFAWDPASTYVRKPTYFDGMQMEPAPVTDI
                     TGARVLAKLGDSVTTDHISPAGSIKADSPAGKYLADNGVDRKDFNSYGSRRGNHEVMI
                     RGTFANIRLKNLLLDGVEGGFTRNFLAGGEQTTIYDASAAYQEAGIPLVVLAGKEYGS
                     GSSRDWAAKGTALLGVKAVIAESYERIHRSNLIGMGVLPLQYAKGTNAESLGLTGTET
                     ISVSGVTALNDGGSPRTLTVTVTREDGSQTSFDAVLRIDTPGEADYYRNGGILQYVLR
                     SLV"
BASE COUNT          346 a          735 c          844 g          368 t
ORIGIN      
        1 cccggtcggg tgatcggctg ttcaccgagg gtgagccgat ggtgccgatg acgttgctgc
       61 gctccaaggc tctgcccacc ggcgtgctgg ccctggcgta tgccccggtg cgcgccgaag
      121 cctaggcaac ccgacgccgg gccctcccgg catccgccgt cccccacaag cactcgtggc
      181 cgcactctca tcgagtgcgg ccacgagtgg ttcctgccag aggcaggctg cgctcagacg
      241 agcgagcgca gaacgtactg gaggatgccg ccgttgcggt agtagtcggc ctcacccggg
      301 gtgtcgatgc gcaggaccgc gtcgaagctg gtctgcgagc cgtcctcgcg agtcacggtc
      361 accgtgaggg tgcgcgggct gccgccgtcg ttgagggcgg tgaccccgga gaccgagatg
      421 gtctcggtgc ccgtgaggcc cagcgactcg gcgttggtgc ccttggcgta ctgcaggggc
      481 agcacaccca tgccgatgag gttggagcgg tggatgcgct cgtaggactc ggcgatgacc
      541 gccttgaccc cgagcagggc cgtgcccttg gccgcccagt cacgcgagga ccccgagccg
      601 tactccttgc cggcgaggac gacgagcggg atgccggcct cctggtaggc ggcggacgcg
      661 tcgtagatcg tcgtctgctc gccacccgcg aggaagttgc gggtgaagcc accctcgacg
      721 ccgtcgagga ggaggttctt caggcggatg ttcgcgaacg tcccgcggat catcacctcg
      781 tggttgccgc gacgggagcc gtaggagttg aagtccttgc ggtcgacccc gttgtcagcc
      841 aggtacttgc ctgccgggct gtcggccttg atggaaccgg ccgggctgat gtggtcggtc
      901 gtgaccgagt cgccgagctt ggcgagcacg cgggcaccag tgatgtcggt gaccggcgcc
      961 ggctccatct gcatgccgtc gaagtacgtg ggcttgcgca cgtaggtcga ggccgggtcc
     1021 caggcgaagg tgtcaccctc gggggtcggc agcgactgcc agcgctcgtc accggcgaag
     1081 acgtcggcgt agtcggccgt gaacaggtca cgggtgatcg acgacgcgat gacggcctcg
     1141 acctcctgcg gcgcgggcca gatgtcggcg aggaagacgt cgttgccggc ctcgtcctga
     1201 ccgagcgggt cggtgtcgaa gtcgaagtcc atcgtgccgg ccagcgcgta ggcgatgcac
     1261 agcggcggcg acgcgaggta gttcatcttg acgtcggggt tgatgcggcc ctcgaagttg
     1321 cggttgccag agagcaccgc agccaccgac aggtcctcgg cctggatcgc cgacgagacg
     1381 tacgccggca gcgggccgga gttgccgatg cacgtggcgc agccgtagcc gacgaggttg
     1441 aagccgagct tgtcgaggta gggccacagg cccgcagcct cgtagtagcc ggtgacgacc
     1501 ttgctaccgg gcgccatcga ggtcttgacc cagggcttga cggtcaggcc ccgctcgacg
     1561 gcgttcttcg cgagcagcgc cgcagccatc atcaccgacg ggttcgaggt gttggtgcac
     1621 gaggtgatgg aggcgatgac gacggcgccg tccttgaggt cgaacaacga ggtgccacct
     1681 cgcccggtgt cctcgccccc ggcctggacc ggcaccgagc gcagttcacc gtcgacgccg
     1741 tagttcttca cgtcgagcgc gaactgctgc ttggaatcgc tgagggcgat gcggtcctgg
     1801 ggtcgcttcg ggccggcgat ggagggaacg accgtcgaca ggtcgagctc gagcttctcg
     1861 gagaaccgcg gctcggccgc cgggtcgagc cacatgccct gctccttggc gtaggcctcg
     1921 acgagagcga cctgctcctc gtcgcggccg gtgaggcgca ggtagtccag ggtgatgtcg
     1981 tcgatgggga agatggcgca ggtcgagccg aactcggggc tcatgttgcc gatcgtcgcg
     2041 cggttggcga gcggcagcgc ggcgactccc tcaccgtaga actcgacgaa cttcccgacg
     2101 acaccgtgct ggcgaagctg ctcggtgatg gtgagcacga cgtcggtggc ggtgacgccc
     2161 gcacgggcgg cgccggtgag cttgaagccg acgacgcgcg ggatgagcat cgagacgggc
     2221 tggccgagca tggccgcttc ggcctcgatg ccgccgacgc cccagccgag cacaccgagg
     2281 ccgttgacca tcg
//
LOCUS       DAOSOC010000263         2287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_47090_length_2287_cov_8.350806, whole genome shotgun sequence.
ACCESSION   DAOSOC010000263 DAOSOC010000000
VERSION     DAOSOC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47090_length_2287_cov_8.350806"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="PL014_12780"
     CDS             <1..1226
                     /locus_tag="PL014_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT58011.1"
                     /translation="INTHFHADFVSGHLELARETGAWIGYGEAAVADFPVRHLADGER
                     ISLGEVTLEVMATPGHTPESISVLVFEHADDEVAYGVLTGDALFIGDVGRPDLLASVG
                     VTADELGVMLYDSIQRKLMGLPDAVRVFPAHGAGSACGKNLSTETQSTIGEQRAFNYA
                     CQPMSQEEFVAVVTEGQPAAPAYFLYNATLNKQERDVRDLDAAVPALTADQVEAALAA
                     GAVVHDARDVQEFASAHLRGSINVPADGRMAETVGMVFSPEQQVVVIAPEGVEQEVAT
                     RFSRIGFDHVVGYVADPEAYFLAHEDDVTRASRLSVAEADAAFARDDVQVVDIRNAGE
                     LESGGVAGATHIPLAELARRSGELDPSRPVVAYCAGGWRSSVAASLLRSKGFADVSDI
                     LGGYGAWERAHATAS"
     gene            1416..>2287
                     /locus_tag="PL014_12785"
     CDS             1416..>2287
                     /locus_tag="PL014_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT58012.1"
                     /translation="MRALTYSAFGATPVVTTLPDPVAPPGGAVVRVTASGLCRSDWHG
                     WMGHDSDIATFPHTPGHEFAGVVEAVGEGVDAGWLGRAVTAPFVLACGECPVCDASAG
                     QVCPNQRQPGFTDPGSFAELVVVRAATTNLVALPDGLDPAMAAGLGCRVATAYRAVAD
                     RARVVGGESVVVIGAGGVGLAAVAVARSRGARVCAVDVSAASLDRALDQGADEVVDAS
                     IGRDKVIAAVTHWAGGGVDVSVDALGSPEACRTGILSLGRRGRHVQVGLLPPAAGRAD
                     IPMERVIGWELDVLG"
BASE COUNT          286 a          875 c          804 g          322 t
ORIGIN      
        1 tcatcaacac ccacttccac gccgacttcg tgtccggcca cctcgagctg gcccgtgaga
       61 ccggcgcctg gatcgggtac ggcgaggccg ccgtcgccga cttccccgtg cgccacctcg
      121 ccgacggcga gcgcatctcg ctcggcgagg tcaccctcga ggtcatggcc acccccggcc
      181 acacccccga gtcgatctcc gtcctcgtct tcgagcacgc cgacgacgag gtggcctacg
      241 gcgtcctcac cggcgatgcc ctcttcatcg gcgacgtcgg ccgccccgac ctgctcgcct
      301 ccgtcggggt gactgccgac gagctcggcg tcatgctcta cgactcgatc cagcgcaagc
      361 tcatggggtt gccggatgcc gtgcgcgtct tcccggccca cggcgccggt tcagcgtgcg
      421 gcaagaacct gtcgaccgag acccagtcga ccatcggtga gcagcgcgcc ttcaactatg
      481 cctgccagcc gatgtcgcag gaggagttcg tcgccgtcgt caccgagggc cagcccgccg
      541 caccggccta cttcctctac aacgcgaccc tgaacaagca ggagcgcgac gtgcgtgacc
      601 tcgacgccgc ggtgccggcc ctcacggccg accaggtgga ggccgcgctg gccgccggtg
      661 cggtcgtgca cgatgcccgc gacgtccagg agttcgcctc ggcccacctg cgcgggtcca
      721 tcaacgtgcc cgcggatggt cggatggccg agacggtcgg catggtgttc tcgcccgagc
      781 agcaggtcgt cgtcatcgcc cccgagggcg tggagcagga ggtcgcgacc cggttctcgc
      841 ggatcggctt cgaccacgtc gtcggctacg tggccgaccc cgaggcctac ttcctggccc
      901 acgaggacga cgtcacgcgg gccagccggc tctccgtggc cgaggccgac gccgccttcg
      961 cccgcgacga cgtgcaggtc gtcgacatcc gcaacgccgg cgagctcgag tccggtgggg
     1021 tggccggggc gacccacatc ccgctggccg aactggcccg ccgctccggt gagctcgacc
     1081 cgagccgccc ggtcgtcgcc tactgcgccg gcggatggcg cagcagcgtc gccgcgagcc
     1141 tgctgcgctc gaagggcttc gccgacgtct ccgacatcct cggcggctac ggcgcgtggg
     1201 agcgcgcgca cgccacagca tcctgacgcc aagccccacc ggggcctgac tccgccgaga
     1261 gtccggtgga tctgacaccg gtcgggggct cccccagaga atcgttgcgg cggtgaagga
     1321 attcgttcac cgccgcaacg gctcccccca gcaccctacg gtgggcgcct cgcccccgcg
     1381 gtcgccgacc cccccacggg tggcagggtt gacccatgcg agccctgaca tactctgcgt
     1441 tcggcgccac ccccgtcgtg accaccctcc ccgacccggt ggcacccccc ggcggcgcgg
     1501 tcgtgcgcgt caccgcgagc ggcctgtgcc gcagcgactg gcacggctgg atgggccacg
     1561 acagcgacat cgccacgttc ccccacaccc cgggccacga gttcgccgga gtcgtcgagg
     1621 ccgtcggcga gggggtggat gccgggtggc tgggtcgcgc cgtcaccgca cccttcgtcc
     1681 tcgcgtgcgg cgagtgcccc gtgtgcgacg cgagcgccgg tcaggtgtgc ccgaaccagc
     1741 gccagccggg cttcaccgac cccggctcgt tcgccgagct cgtcgtcgtg cgcgcggcga
     1801 cgaccaacct cgtggccctg cccgacggcc tcgacccggc gatggcggcc ggcctgggct
     1861 gccgcgtcgc gacggcctac cgggccgtgg ccgaccgagc ccgggtggtc ggcggtgaga
     1921 gcgtcgtcgt catcggtgcc ggcggggtgg ggttggccgc ggtggccgtg gcacgcagcc
     1981 gcggcgcgcg ggtgtgcgcc gtcgacgtct ccgcggcatc cctggaccgg gcactcgacc
     2041 agggcgccga cgaggtggtc gacgcctcga tcggccgtga caaggtgatc gccgccgtca
     2101 cgcactgggc gggcgggggt gtcgacgtga gcgtcgacgc cctgggatcc cccgaggcct
     2161 gccgcaccgg catcctgtcg ttggggcgcc ggggcaggca cgtccaggtg gggctgctgc
     2221 cgcctgccgc gggtcgcgcc gacatcccga tggagcgggt catcgggtgg gagctcgacg
     2281 tgctcgg
//
LOCUS       DAOSOC010000264         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_54793_length_2046_cov_8.433451, whole genome shotgun sequence.
ACCESSION   DAOSOC010000264 DAOSOC010000000
VERSION     DAOSOC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54793_length_2046_cov_8.433451"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1084
                     /locus_tag="PL014_12790"
     CDS             95..1084
                     /locus_tag="PL014_12790"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HOT58013.1"
                     /translation="MSFVAVPFRRPFSVRDPHEPGRAATPLELFFDLIFVVAIASNVV
                     QLHHGITEGHLDAVVGYTLAWFAIWWAWMGYTWYASAYDNDDVVFRLLGASCSSGQFS
                     PRPWPSTSCTPARRSTPSLASMESEGTEAAEGTSAQVLEQARAAIDAGATWRARDLLA
                     AHVEADRDPAVLTMLGEVYTSMGDLPRAGAIWFAAGAKGDEVDAAVSAWRAQSRDDFT
                     LMWWSLPASVRVEPMPRRIEALRERARAAGGDVDAEVSRTASDVVGPRPDGPAGEPAA
                     APSSELTVHPAPDAEEEEGKFDASWFVAWVLAVLFVVFAVIGFVTALGWVVPG"
     gene            1304..1579
                     /locus_tag="PL014_12795"
     CDS             1304..1579
                     /locus_tag="PL014_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT58014.1"
                     /translation="MAGSIPGTLVLDESGCVRLRSADRLVSVLWPDGWTATLDGQEVV
                     ITQPSGEHSLRTGEPVDLGGGFISEEGYEAQRCASGVPWQVVGVAFE"
     gene            1666..>2046
                     /locus_tag="PL014_12800"
     CDS             1666..>2046
                     /locus_tag="PL014_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT58015.1"
                     /translation="MSPDIEPSDARAARRQQARENAEYHRLAAERAAQAEARATAPMV
                     AAFAQAMRAAGIEPTRLRAMPYSGKGNLRTDLDGWYVRRDRRNAVGVDGRWYVLVAAP
                     SLRGRLVGIHVEPSDAPMQVGAGGR"
BASE COUNT          258 a          680 c          788 g          320 t
ORIGIN      
        1 gcttaccagt gtgctaccga ccggtaacta aagcaagggg gatgccgtga cacggagtga
       61 gtggcgtacg tcacgcccgc cccccatcct ccgggtgagt ttcgtcgccg tcccgttccg
      121 ccgccccttc tccgtccggg acccccacga gccgggacgg gccgccaccc ccctcgagct
      181 gttcttcgac ctcatcttcg tcgtggccat cgcgagcaac gtcgtccagc tgcaccacgg
      241 catcaccgag ggtcacctcg acgcggtggt cgggtacacg ctcgcgtggt tcgcgatctg
      301 gtgggcctgg atgggctaca cgtggtacgc ctcggcctac gacaacgacg acgtcgtctt
      361 ccggttgttg ggagcgtcct gctcgtcggg gcagttctcg ccgcggccgt ggccgagcac
      421 gtcctgcact cccgcgaggc ggtcaacgcc tagtctggcg agcatggaga gcgaggggac
      481 cgaggcagcc gagggcacgt ccgcgcaggt gctcgaacag gcgcgcgccg ccatcgacgc
      541 aggtgccacc tggcgggccc gcgacctgct cgccgcccac gtcgaggccg accgcgaccc
      601 cgcggtgctg acgatgctcg gtgaggtcta cacctccatg ggggacctgc cgcgggccgg
      661 tgccatctgg ttcgcggccg gcgccaaggg ggacgaggtg gatgccgcgg tgtcggcctg
      721 gcgggcacag tcccgtgacg acttcacgct catgtggtgg tcgctccccg cgtcggttcg
      781 ggtcgagccg atgccgcgcc ggatcgaggc gctgcgcgag cgggcccgcg cagcgggtgg
      841 tgacgtcgac gcggaggtgt cgcggacggc atccgacgtt gttggtcctc gccccgacgg
      901 cccggccggg gagcctgcgg ccgcgccgag ctccgagctg acggttcacc ccgcccctga
      961 cgccgaggag gaggagggga agttcgacgc ctcgtggttc gtcgcgtggg tgctcgcggt
     1021 gctgttcgtg gtgttcgccg tgatcgggtt cgtgaccgcg cttggctggg tcgtgccggg
     1081 ctgaggcctc atgtgcgact ctggtggcgt gagggcgggt ctctttggca cgaaaggcac
     1141 tgcgatcggg gtcaatgcga tcgcagtgat cgtcgtcatg gcagcggggg cctgctcggg
     1201 ggactcggct ccgcgaacgc cgaccgaccg gcccaccgcc agcccgcaag cgacgtcgaa
     1261 ggagccgctc gcgcggtcca ggtggcggcc cgggcagagc gcgatggccg gctcgatccc
     1321 gggcacgctc gtgctggacg agagcggctg cgtccggctg cggtcggctg accggctggt
     1381 gtccgtcctc tggccggatg ggtggaccgc gacgctggat ggacaggagg tcgtgatcac
     1441 ccagccgtcc ggcgagcaca gcctgcgcac gggggagccc gtggaccttg gtggcggctt
     1501 catcagcgag gaaggctacg aggcgcagcg ctgtgcttcg ggggttcctt ggcaggtcgt
     1561 cggggtggca ttcgagtagt cagcgggtgc gctgatttcc ggtcgcggtg ttcggcggtg
     1621 cgggtctgcc cggcggtggc gcgtcggggc tgggatgatc ggcgggtgag cccggacatc
     1681 gagccgtcag acgccagagc tgcccgtcgc cagcaggcgc gcgagaacgc cgagtaccac
     1741 cgtctcgctg ccgagcgggc agcgcaggcc gaggcgcgtg ccacggcacc gatggtggcg
     1801 gccttcgccc aggcgatgcg ggccgccggc atcgagccca cccgcttgcg ggcgatgccg
     1861 tacagcggca aaggcaacct gcgcacggac ctcgacgggt ggtacgtgcg gcgtgatcgg
     1921 cgcaacgccg tgggggtgga cggccgctgg tacgtgctcg tcgcggcgcc cagcctgcgc
     1981 ggccggctgg tcggcatcca cgtcgaaccg agtgacgccc cgatgcaggt cggtgccggt
     2041 gggcgg
//
LOCUS       DAOSOC010000265         2022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_55708_length_2022_cov_12.503813, whole genome shotgun
            sequence.
ACCESSION   DAOSOC010000265 DAOSOC010000000
VERSION     DAOSOC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55708_length_2022_cov_12.503813"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..621)
                     /locus_tag="PL014_12805"
     CDS             complement(61..621)
                     /locus_tag="PL014_12805"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOT58016.1"
                     /translation="MTGRLATTGLLGAAAVAFALGPVAVPNYSLVSQSISETAAQATP
                     GAWVARSGFALLGMAVLSARSHAQVGRPSAMAYSAFALGLFGVGVWSHEPYLADLPYD
                     ATEASLHSLVAAGMGLAIVTAEVLAAWHTRRKGPLLLASAYLLLPAAMNLHPAFAGVY
                     QRLMFAILIASLLVRTWRPAAAQPVQ"
     gene            complement(618..1253)
                     /locus_tag="PL014_12810"
     CDS             complement(618..1253)
                     /locus_tag="PL014_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT58017.1"
                     /translation="MTTTMTSVPATSGLLRIRRLAAGAALGALGALLVGGALMAVPRR
                     GLDGNALVGGLVLLGPAALAAVSLLDMFTVPGLHQTLHRHGPVLVAVATGCALAGDLL
                     GVIGRLTQLAAVVSQGQQRPDVQTLVLDLLQATFNAGGFVLVAISFTAFGVLFIRNRS
                     RRLGAVAVAAGVLTGLGQLPGLQVLFYLANVFFLAWYITLGVTFRRTEASS"
     gene            1376..1945
                     /locus_tag="PL014_12815"
     CDS             1376..1945
                     /locus_tag="PL014_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT58018.1"
                     /translation="MRQRLVDACWQGLLDASAGGPGVTVATICAEADCTPPTLYHHFA
                     TLDELKLTTCRQAFQEWADSLAKVIGREPDPAVRLHLRGRAYVTWGADHPAAYRALFL
                     MPQAENADPSPGPGFDPLVADLSLVLDRDPADPAVTTAAFAHWSAVHGLTSLVIASPS
                     LTAPRWEPVLTYLSRALLLGAGDLPPASP"
BASE COUNT          331 a          712 c          716 g          263 t
ORIGIN      
        1 gatggccacc agcccaccgt aggtgcgagc gcgccgcccg tgcaccccga tttcgcggcg
       61 ctactgcacc ggctgggcgg cggccgggcg ccaggtgcgc acgagcaggc tggcgatgag
      121 gatggcgaac atcagtcgct ggtagacccc ggcaaacgcc gggtggaggt tcatcgcggc
      181 cgggagcagg agataggcgc tcgcgagcag gagtggaccc ttgcggcggg tgtgccaggc
      241 cgcgagaacc tctgccgtga cgatcgccag ccccatgccg gcggcgacga ggctgtgcag
      301 gctcgcctcg gtggcgtcgt agggcaggtc ggccaggtag ggctcgtgcg accacacccc
      361 gacgccgaac aggcccaggg cgaacgcgga gtaggccatc gcgctgggcc tccccacctg
      421 agcatgcgag cgggccgaca ggaccgccat tccgaggagc gcgaagccgc tgcgggcgac
      481 ccaggctccc ggcgtcgcct gtgctgccgt ctcgctgatg ctctggctga ccagggagta
      541 gttcggcacc gcgacgggac cgagcgcgaa ggcgaccgct gccgccccga gcagcccggt
      601 cgtcgcaagc cggcccgtca tgacgacgcc tccgtccggc ggaaggtgac gcccagcgtg
      661 atgtaccacg caaggaagaa cacgttggcc aggtagaaga gcacctggag acccggaagc
      721 tgccccaacc cggtcaggac gcctgcggcg accgccacgg ctccgagacg ccgtgacctg
      781 tttctgatga acaggacgcc gaaggccgtg aaggagatcg ccaccaggac gaagccaccc
      841 gcgttgaagg tggcctgcag cagatccagc acgagggtct ggacgtccgg ccgttgctga
      901 ccctgactca ccacggcggc gagttgggtc agccgaccga tcacaccgag caggtcaccg
      961 gcgagcgcac acccggtcgc caccgcaacg aggactgggc cgtgccgatg cagggtctga
     1021 tgcaggcccg gcacggtgaa catgtcgagc aggctgaccg ccgcgagggc cgcggggccg
     1081 agcagcacca gacctcccac cagggcgttg ccgtccaggc ctcgacgtgg gactgccatg
     1141 agcgcgccgc cgacgagcag cgccccgagg gcaccgagcg ccgcgcctgc ggccaggcga
     1201 cggattctga ggaggcccga cgtggcgggc acggatgtca tcgtggtcgt catgtaaata
     1261 tagtagcgct atatatttgg cccggcagaa tggggtagga gccaagaacc gatcggaggg
     1321 gcgagtgccg gagcgacgca ggcgtgggcg cccgacgacc gacgcgggct tcggcatgcg
     1381 ccaacgcctc gtcgacgcct gctggcaagg actcctggat gcctcggcgg gcggaccggg
     1441 ggtcacggtg gcgaccatct gcgcggaagc ggactgcacg cccccgaccc tctatcacca
     1501 cttcgccacc ctcgatgagc tcaagctgac gacctgccgt caggccttcc aggagtgggc
     1561 ggattcactg gcgaaggtga tcgggcgtga gccggatccc gcggttcggc tgcacctgcg
     1621 tgggcgggcc tatgtcacgt ggggagccga ccaccccgcc gcctatcgag cgctcttcct
     1681 catgccccag gccgagaacg ctgacccctc ccccggaccg gggttcgatc cgctcgttgc
     1741 ggacctctcg ctcgtgctgg atcgagaccc ggccgatcca gccgtcacca ccgcggcctt
     1801 cgcccactgg agcgcggtcc acgggctcac gagcctcgtc atcgccagcc cgagcctcac
     1861 cgcgccccgc tgggagccgg tgctcaccta cctgtcccgg gccctgctcc tcggggccgg
     1921 ggatctcccg ccggcctcgc cgtaaaccgc gcccggccgg gcttagtcct cgtcgtcgag
     1981 ggggcagccc acgacgatga tgctgcgggc ctggacggtg ac
//
LOCUS       DAOSOC010000266         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_57220_length_1982_cov_8.441619, whole genome shotgun sequence.
ACCESSION   DAOSOC010000266 DAOSOC010000000
VERSION     DAOSOC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57220_length_1982_cov_8.441619"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PL014_12820"
     CDS             <1..639
                     /locus_tag="PL014_12820"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOT58019.1"
                     /translation="PDLDAAGHELLLSVDRHGLAGLPTSASTANVSAVDSDGTACTIT
                     VSSGYGAGITVPGTGLLLNNALGEPELNRLGLHKMAPGIRLASNMAPTTGRTRDGRVL
                     AIGSPGADRITTALMQVLGQGCLHGAELAHAIEAPRLHVSFDADGTPVVDHESDDAIA
                     DAVRGLGLRGTDHGRHQMFFGGVGAAFRHADGVVEAAGDPRREAAVEVVARA"
     gene            complement(644..>1982)
                     /locus_tag="PL014_12825"
     CDS             complement(644..>1982)
                     /locus_tag="PL014_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT58020.1"
                     /translation="GQHREVLRLAIPAFLALVAEPLFLLTDAAIVGRLGVEPLAGLGV
                     ASAILLTAANIFVFLAYGTTAVVARQLGAGDRPGAIAGGIDGTWLAITLGTATAAAVA
                     TAAEPLCRVFGASEGALEQAVIYLRISALGIPAMLVVLATTGVLRGLQDTTTPLIAAV
                     AGFGLNIVLNLWFVHGLGWGIAGSAWGTVIAQTVMAIGLVTVLIRYARRDCVPLRAHP
                     GRVLIAARGGIPLLVRTLALRGVLLVTTWVAASMGDVPLAAYQVTATIWTFFAFALDA
                     LAIAAQAITGKSLGAGDREKAREATALMLRWGVLGGVVLGALLLLTRPFLAPLFTPDA
                     EVQAAIAAGLLVVAIGQPLSGLVFVIDGVLIGAGDGVWLAKAMLVTLLAYLPIVAAAH
                     AVGGRVVDGTNAGDATAVTWLWVAFTAFMAVRAALMWLRVRTGHWMVLGATRP"
BASE COUNT          309 a          671 c          776 g          226 t
ORIGIN      
        1 cctgacctcg acgcggcggg gcacgaactg ctgctgtccg tcgaccggca cgggttggcc
       61 ggcctgccca cgagcgccag cacggcgaac gtctcggcgg tcgactccga cggcaccgcc
      121 tgcacgatca ccgtgtccag cgggtacggc gcggggatca ccgtccccgg caccgggctg
      181 ctcctcaaca acgcgctcgg cgagccagaa ctcaaccggc tcggactgca caagatggct
      241 cccggcatcc gactcgcctc caacatggcc ccgacgaccg ggcgaacccg cgacggccgg
      301 gtgctcgcga tcggatcacc cggcgccgac cggatcacga ccgcgctcat gcaggtgctg
      361 gggcagggat gcctgcacgg cgcggagctc gcccacgcga tcgaggcgcc gcggctgcac
      421 gtcagcttcg acgccgacgg cacccccgtg gtcgaccacg agagcgacga cgcgattgcg
      481 gatgccgtgc gcgggctggg attgcgcggc accgaccacg gtcgtcacca gatgttcttc
      541 gggggtgtcg gggcggcgtt ccgccatgcc gatggggtgg tggaggctgc cggggatccg
      601 cgccgcgagg ctgcggtcga ggtcgtcgca cgggcctgac gggtcagggg cgggtggcgc
      661 cgaggaccat ccagtggccg gtgcgaaccc gcagccacat gagggcggcg cgcacggcca
      721 tgaaggcggt gaacgccacc cacagccagg tcaccgcagt ggcgtcgccg gcgttggtgc
      781 catcgacgac ccggccgccg accgcgtggg cggcggccac gatcgggagg taggccagga
      841 gggtcacgag catggccttg gccagccaga ccccgtcgcc cgcgccgatg agcaccccgt
      901 cgatgacgaa cacgagcccg gagagtggtt gtccgatggc cacgacgagg agcccggcgg
      961 cgatggcggc ctgcacctcg gcatccggcg tgaacagcgg agcgaggaag ggtcgggtga
     1021 gcaggagcag ggcaccgagg accacgccgc ccagcactcc ccagcgcagc atcagcgcgg
     1081 tcgcctcgcg ggccttctcg cggtcgccgg cgcccaggga cttgccggtg atggcctgtg
     1141 ccgcgatggc gagggcgtcg agggcgaagg cgaagaaggt ccagatcgtc gctgtcacct
     1201 ggtaggccgc gaggggcacg tcccccatgc tcgcggccac ccacgtggtg acgagcagca
     1261 cgccgcgcag cgccaacgtg cgcacgagca gggggatgcc gccgcgcgcc gcgatgagga
     1321 cgcggccggg gtgggcccgc aggggcacgc agtcgcgccg tgcgtagcgg atgagcaccg
     1381 tgaccagtcc gatggccatc acggtctggg cgatgaccgt gccccaggcg gagccggcga
     1441 tgccccagcc gaggccgtgc acgaaccaca ggttgagcac gatgttgagg ccgaagcctg
     1501 cgacggcggc gatgagtggg gtcgtggtgt cctggaggcc gcgcagcacg ccggtcgtcg
     1561 ccaggacgac gagcatggcc gggatgccga gggcggagat gcgcaggtag atcactgcct
     1621 gctcgagggc gccctcggag gcgccgaaga cccggcagag cggttcggcg gctgtcgcca
     1681 cggctgcggc tgttgccgtg cccagggtga tggcgagcca ggtgccgtcg atgcccccgg
     1741 cgatggcacc gggacgatcg cccgcgccga gctggcgggc gacgacggcg gtggtgccgt
     1801 aggcgaggaa gacgaagatg ttggcggcgg tcagcaggat ggcgctggcg acgccgagac
     1861 cggccagtgg ctcgacgccg aggcgcccga cgatggcggc gtcggtgagc aggaacagcg
     1921 gctcggcgac cagggcgagg aaggccggga tggccaggcg cagcacctcg cggtgctgcc
     1981 cg
//
LOCUS       DAOSOC010000267         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_59495_length_1929_cov_8.639274, whole genome shotgun sequence.
ACCESSION   DAOSOC010000267 DAOSOC010000000
VERSION     DAOSOC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59495_length_1929_cov_8.639274"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PL014_12830"
     CDS             <1..1182
                     /locus_tag="PL014_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT58021.1"
                     /translation="PPWFTRDRAVWRTVAVAVVAVAALAWVVTRLGPLDVTSNLMDPS
                     RGGLLVVLALGLAAGVSTCMAMVGGLVLGFSAAHAAALAESGRSDLPFRVRMRPQLAF
                     NTGRVVGFGVLGALLGAIGSTMSPPTRVLAVLVLGVAVVMFLLGVRLTGISPRVAAWS
                     PRLPGGLARALGIDTATQRSYSHTRTALIGAATFLLPCGFTQAVQLYALSTASPVTAG
                     VIMATFALGTAPGLLALGSVPELTTGARQAMVLRVIGVVVLAFGLLNVSSGLNLLGLG
                     GLGGGSAAAGARTASANVTVTDGIQTVRMTQNPDGYEPADTVLYADLPTRWVIEGTSP
                     FDCSAFVRVPDLDLRVDLAMGPNTVELPPLDTGEVPFTCVMGMYSGTLTAIDQPSGGA
                     G"
     gene            1319..>1929
                     /locus_tag="PL014_12835"
     CDS             1319..>1929
                     /locus_tag="PL014_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT58022.1"
                     /translation="MITVSSLTKTYGGFTAVDDVSFTAQTGRVTGFLGPNGAGKSTTM
                     RIMVGLTPASTGTALIDGRRFVDLPNPGAEVGVLLDASAQHAGRTGREILTIAQRTMG
                     LPKARVQDMLELVSLTDEEADRRVRNYSLGMRQRLGIGTALLGDPHVLILDEPANGLD
                     PAGIRWMRDLLRGYADRGGTVLLSSHLLHEIEVIADDLVVIGNG"
BASE COUNT          237 a          757 c          637 g          298 t
ORIGIN      
        1 cccccgtggt tcacccgtga ccgggcggtg tggcgcacgg tcgccgtcgc cgtcgtcgcg
       61 gtggccgcgc tcgcctgggt ggtcacccgc ctcggcccgc tcgacgtcac ctcgaacctc
      121 atggacccct cgcgtggcgg cctgctcgtc gtgctcgccc tggggctggc cgccggggtg
      181 tcgacctgca tggccatggt cggcggcctg gtgctcggat tctccgcggc gcacgcggca
      241 gcgctcgccg agtcgggccg cagcgacctg ccgttccgcg tccggatgcg accccagctc
      301 gcgttcaaca ccggccgggt cgtcgggttc ggcgtgctcg gcgccctgct cggcgcgatc
      361 ggctcgacga tgagcccgcc cacgcgcgtg ctggccgtgc tcgtcctcgg ggtcgccgtc
      421 gtgatgttcc tgctcggggt ccgcctcacc ggcatctccc cccgggtggc ggcctggtcg
      481 ccgcggcttc ccggggggct tgctcgtgcc ctgggcatcg acacggcgac gcagcgctcc
      541 tacagccaca cccgcacggc actcatcggc gctgcgacgt tcctcctgcc gtgcggcttc
      601 acccaggccg tccagctcta cgcgctgtcg accgcgtccc cggtgacggc cggggtcatc
      661 atggcgacct tcgcgctcgg caccgctccg ggcctgctcg cgctcggctc ggtgcccgag
      721 ctgaccaccg gcgcacgcca ggccatggtg ctgcgggtga tcggtgtcgt cgtcctcgcg
      781 ttcggcctgc tcaacgtctc cagcgggctc aacctccttg gcctcggagg gctgggaggt
      841 gggtcggcag cggccggcgc gcggacggca tccgccaatg tcacggtgac agacggcatc
      901 cagacggtgc ggatgaccca gaacccggat ggctacgagc cggcggacac cgtgctctac
      961 gccgacctac ccacccgctg ggtcatcgag ggcacgtccc cgttcgactg cagtgccttc
     1021 gtgcgcgttc ccgacctcga cctccgagtc gacctcgcca tgggcccgaa caccgtcgag
     1081 ctgccacccc tggacaccgg tgaggtgccg ttcacctgcg tcatggggat gtactcaggc
     1141 accctcaccg ccatcgacca gcccagcggc ggagccggct gaccccacga cccaggtgcg
     1201 gtggtgggcg acccatcacg gtcggcgcgg tacctgaggg aactcagggt cggctcaggg
     1261 tcggtctcag gtgtgacccc gatgaccgcg ggccaccgtg accggcaggc tttcccccat
     1321 gatcactgtc agctcgctca ccaagaccta cggcggcttc acggccgtcg acgacgtctc
     1381 cttcaccgcg cagaccggcc gcgtcacggg cttcctcggc ccgaacggcg ccggcaagtc
     1441 gacgaccatg cggatcatgg tcggcctcac cccggcctcg accgggaccg ccctcatcga
     1501 cggccgccgg ttcgtcgacc tgcccaaccc cggtgccgag gtgggggtcc tcctcgacgc
     1561 ctccgcccag cacgccgggc gcaccggccg agagatcctc accatcgccc agcgcaccat
     1621 ggggctgccc aaggcccgcg tgcaggacat gctcgagctc gtcagcctca ccgacgagga
     1681 ggccgaccgg cgggtgcgca actactcgct cggcatgcgc cagcgcctcg gcatcggcac
     1741 cgcgctgctc ggcgacccac acgtcctgat cctcgacgag cccgccaacg gcctggaccc
     1801 ggccggcatc cgctggatgc gcgacctcct gcgcggctac gccgaccgtg gcggcacggt
     1861 cctgctgtcc tcgcacctgc tgcacgagat cgaggtcatc gccgacgacc tcgtcgtcat
     1921 cggcaacgg
//
LOCUS       DAOSOC010000268         1854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Ornithinibacter sp. isolate AMR_MDS_2355
            NODE_62912_length_1854_cov_7.011673, whole genome shotgun sequence.
ACCESSION   DAOSOC010000268 DAOSOC010000000
VERSION     DAOSOC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864422
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ornithinibacter sp. (wastewater metagenome)
  ORGANISM  Ornithinibacter sp.
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Micrococcales;
            Intrasporangiaceae; Ornithinibacter.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:56:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,570
            CDSs (total)                      :: 2,537
            Genes (coding)                    :: 2,526
            CDSs (with protein)               :: 2,526
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Ornithinibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62912_length_1854_cov_7.011673"
                     /isolate="AMR_MDS_2355"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2862748"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      <1..54
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            567..884
                     /locus_tag="PL014_12840"
     CDS             567..884
                     /locus_tag="PL014_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOT58023.1"
                     /translation="MNIARGLLGIPYVYGGSTPSGFDCSGFTQYVFGKAGISIPRTAS
                     AQQRAATRVSNPKPGDLVFFGSPAWHVGIYTGDGMMIDSPKPGKSTSERPVFSGVSGY
                     GRF"
     gene            complement(968..1408)
                     /locus_tag="PL014_12845"
     CDS             complement(968..1408)
                     /locus_tag="PL014_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOT58024.1"
                     /translation="MDFTTELEATGGKTTGFVVPDAVVDALGAGRRPKVAVTVNGHTW
                     RTSIAPMGGRYLVGASAAVRAAAGIAAGETHTVTVEVDDTPRTVEVPDELAAALAASP
                     TAAAAWAALSYSQQRRHADPLAAAKAPETRARRVAATMAALTGP"
     gene            complement(1494..>1854)
                     /locus_tag="PL014_12850"
     CDS             complement(1494..>1854)
                     /locus_tag="PL014_12850"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOT58025.1"
                     /translation="VTVNGRAVLAFESSLTWDINRVEGASVMSGGLFNTTFTGRGMIA
                     VVTHGTPVVLQVDEPTHVDMQAAVLWSAGLQSSIRKTAKLGAVIGRGSGEAYQLALQG
                     HGIVVVQASEGHPIPQQ"
BASE COUNT          253 a          698 c          641 g          262 t
ORIGIN      
        1 cgggcaacct cagcccgaac ccgacagctc accccgtagg cactgagagg ctcactgtgc
       61 tcgacactgc cacttcgggg cgccaccgcg gcgccgcccg gtacaacccc tggtccgaac
      121 tctcgaccat cgtcaggagc gctggccaga acggcgtcaa ggccacggcc gtcctcgctg
      181 cctccggcgg actggtcgcg accttcgccc tcccggccca ggccgagccg cgtgccgacg
      241 ccgccgcagc tgctgaggcc gccgcagccc ccgccgcagt cgcagccccg gtggcgctgg
      301 caccgtcggt cgtcgcgccg gccgtcgcag ccccggtggc caccgaggtc gtgggtgtcg
      361 tcggcgtcac cgccgtcgcc aagcccaagc ccaagccgcg ccccgtcgtg gtgcaggcgc
      421 aggcggatgc cgacgaggtc gccactcgcc gcagcaacac ctccgcctcc cgcagcaccc
      481 ccgcccgctc gaccacgagc accaagacgg cctcgaccgc gaagacggcg acgtccagca
      541 acgtcccgca gtccgccagt ggcgtggtga acatcgcccg cggcctgctc ggcatcccct
      601 acgtctacgg cggctcgacc ccttccgggt tcgactgctc cgggttcacc cagtacgtct
      661 tcggcaaggc agggatctcc atcccgcgca cggcctcggc ccagcagcgt gcggccacca
      721 gggtcagcaa ccccaagccc ggtgacctcg tgttcttcgg ttccccggcc tggcacgtgg
      781 gcatctacac cggcgacggc atgatgatcg actcgcccaa gcctggcaag tccacctccg
      841 agcgtccggt gttctccggc gtcagcgggt acggccgctt ctgatccgcc accgacctca
      901 gatcgaggtg ccaccccgcg ggaagccgtg gagtggcacc tcgatcggca tctccggggt
      961 ggacggctca ggggccggtg agcgcggcca tcgtcgcggc aacccggcgc gcccgggtct
     1021 cgggggcctt ggcggcggcc aggggatccg cgtgccttcg ctgctggctg taggacaggg
     1081 cggcccatgc ggctgccgcg gtcggggatg ccgcgagcgc tgcggcgagc tcgtcgggca
     1141 cctccaccgt gcgcggtgtg tcgtcgacct caaccgtcac ggtgtgcgtc tcacccgcgg
     1201 cgatgcccgc tgcggcgcgt accgccgcgc tggcacccac gaggtagcgc ccacccatgg
     1261 gagcgatgct cgtgcgccag gtgtggccgt tgacggtcac ggcgaccttg ggacgacgcc
     1321 cggcacccaa ggcgtcgacg acggcatccg gaacgacgaa cccggtggtc ttgccgccgg
     1381 tcgcctcgag ctcggtggtg aagtccatga ctggagtatg cgcccccggc agagggcgtc
     1441 ggcggttcct ggttcccggt cgggtgcggt gaggggctga ccccgggcgc cggctactgc
     1501 tgggggatcg ggtggccctc ggaggcctgg acgacgacga tgccgtgacc ctgcagcgca
     1561 agctggtagg cctcgcccga gccgcggccg atgacggctc cgagcttggc ggtcttgcgg
     1621 atcgaggact ggagaccggc cgaccacagc accgcggcct gcatgtcgac gtgggtcggc
     1681 tcgtcgacct ggagcaccac cggcgtgccg tgggtgacca cggcgatcat cccgcggccg
     1741 gtgaaggtgg tgttgaacag gccgccgctc atcaccgagg caccctcgac gcggttgatg
     1801 tcccacgtca ggctgctctc gaacgcgagg accgctcggc cgttgaccgt gacg
//
